LOCUS       DAHHZJ010000001        52116 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.001, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000001 DAHHZJ010000000
VERSION     DAHHZJ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.001"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            302..1213
                     /locus_tag="NBL02_000001"
     CDS             302..1213
                     /locus_tag="NBL02_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCD2570720.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(1591..2508)
                     /locus_tag="NBL02_000002"
     CDS             complement(1591..2508)
                     /locus_tag="NBL02_000002"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HCD2570721.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            2710..3147
                     /gene="zapA"
                     /locus_tag="NBL02_000003"
     CDS             2710..3147
                     /gene="zapA"
                     /locus_tag="NBL02_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCD2570722.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            3205..3753
                     /locus_tag="NBL02_000004"
     CDS             3205..3753
                     /locus_tag="NBL02_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HCD2570723.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            3868..6228
                     /locus_tag="NBL02_000005"
     CDS             3868..6228
                     /locus_tag="NBL02_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HCD2570724.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            6315..6629
                     /gene="trxA"
                     /locus_tag="NBL02_000006"
     CDS             6315..6629
                     /gene="trxA"
                     /locus_tag="NBL02_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HCD2570725.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            6811..8589
                     /gene="uvrC"
                     /locus_tag="NBL02_000007"
     CDS             6811..8589
                     /gene="uvrC"
                     /locus_tag="NBL02_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCD2570726.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            8734..9633
                     /locus_tag="NBL02_000008"
     CDS             8734..9633
                     /locus_tag="NBL02_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2570727.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            9626..10888
                     /locus_tag="NBL02_000009"
     CDS             9626..10888
                     /locus_tag="NBL02_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2570728.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            11049..12947
                     /locus_tag="NBL02_000010"
     CDS             11049..12947
                     /locus_tag="NBL02_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HCD2570729.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(13277..13996)
                     /locus_tag="NBL02_000011"
     CDS             complement(13277..13996)
                     /locus_tag="NBL02_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HCD2570730.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            14333..14665
                     /locus_tag="NBL02_000012"
     CDS             14333..14665
                     /locus_tag="NBL02_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2570731.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            14652..16655
                     /locus_tag="NBL02_000013"
     CDS             14652..16655
                     /locus_tag="NBL02_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HCD2570732.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            16674..17144
                     /locus_tag="NBL02_000014"
     CDS             16674..17144
                     /locus_tag="NBL02_000014"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HCD2570733.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            17165..17749
                     /locus_tag="NBL02_000015"
     CDS             17165..17749
                     /locus_tag="NBL02_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HCD2570734.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            17764..18750
                     /locus_tag="NBL02_000016"
     CDS             17764..18750
                     /locus_tag="NBL02_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HCD2570735.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            18740..19051
                     /locus_tag="NBL02_000017"
     CDS             18740..19051
                     /locus_tag="NBL02_000017"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HCD2570736.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            19073..20854
                     /locus_tag="NBL02_000018"
     CDS             19073..20854
                     /locus_tag="NBL02_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HCD2570737.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            20847..22223
                     /locus_tag="NBL02_000019"
     CDS             20847..22223
                     /locus_tag="NBL02_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HCD2570738.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            22230..22865
                     /locus_tag="NBL02_000020"
     CDS             22230..22865
                     /locus_tag="NBL02_000020"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HCD2570739.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            22895..24250
                     /locus_tag="NBL02_000021"
     CDS             22895..24250
                     /locus_tag="NBL02_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HCD2570740.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(24442..25836)
                     /locus_tag="NBL02_000022"
     CDS             complement(24442..25836)
                     /locus_tag="NBL02_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCD2570741.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            25928..26899
                     /locus_tag="NBL02_000023"
     CDS             25928..26899
                     /locus_tag="NBL02_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HCD2570742.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            26896..27678
                     /locus_tag="NBL02_000024"
     CDS             26896..27678
                     /locus_tag="NBL02_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HCD2570743.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(27847..28218)
                     /locus_tag="NBL02_000025"
     CDS             complement(27847..28218)
                     /locus_tag="NBL02_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCD2570744.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            28352..28795
                     /locus_tag="NBL02_000026"
     CDS             28352..28795
                     /locus_tag="NBL02_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HCD2570745.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(28838..29647)
                     /gene="yidA"
                     /locus_tag="NBL02_000027"
     CDS             complement(28838..29647)
                     /gene="yidA"
                     /locus_tag="NBL02_000027"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCD2570746.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(29666..31033)
                     /locus_tag="NBL02_000028"
     CDS             complement(29666..31033)
                     /locus_tag="NBL02_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCD2570747.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(31017..31847)
                     /locus_tag="NBL02_000029"
     CDS             complement(31017..31847)
                     /locus_tag="NBL02_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HCD2570748.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            32010..32447
                     /locus_tag="NBL02_000030"
     CDS             32010..32447
                     /locus_tag="NBL02_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HCD2570749.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            32523..33143
                     /locus_tag="NBL02_000031"
     CDS             32523..33143
                     /locus_tag="NBL02_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HCD2570750.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            33332..33838
                     /locus_tag="NBL02_000032"
     CDS             33332..33838
                     /locus_tag="NBL02_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HCD2570751.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            33855..34754
                     /locus_tag="NBL02_000033"
     CDS             33855..34754
                     /locus_tag="NBL02_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HCD2570752.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            34841..35725
                     /locus_tag="NBL02_000034"
     CDS             34841..35725
                     /locus_tag="NBL02_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCD2570753.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(35917..35988)
                     /locus_tag="NBL02_000035"
     tRNA            complement(35917..35988)
                     /locus_tag="NBL02_000035"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35953..35955),aa:Arg,seq:ccg)
     gene            36048..37382
                     /gene="rpoN"
                     /locus_tag="NBL02_000036"
     CDS             36048..37382
                     /gene="rpoN"
                     /locus_tag="NBL02_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCD2570754.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            37531..38622
                     /locus_tag="NBL02_000037"
     CDS             37531..38622
                     /locus_tag="NBL02_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HCD2570755.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            38663..39664
                     /gene="gap"
                     /locus_tag="NBL02_000038"
     CDS             38663..39664
                     /gene="gap"
                     /locus_tag="NBL02_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCD2570756.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            39774..40964
                     /locus_tag="NBL02_000039"
     CDS             39774..40964
                     /locus_tag="NBL02_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCD2570757.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            41068..41823
                     /locus_tag="NBL02_000040"
     CDS             41068..41823
                     /locus_tag="NBL02_000040"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCD2570758.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            41939..43237
                     /gene="eno"
                     /locus_tag="NBL02_000041"
     CDS             41939..43237
                     /gene="eno"
                     /locus_tag="NBL02_000041"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCD2570759.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            43637..44776
                     /locus_tag="NBL02_000042"
     CDS             43637..44776
                     /locus_tag="NBL02_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HCD2570760.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(44843..45418)
                     /locus_tag="NBL02_000043"
     CDS             complement(44843..45418)
                     /locus_tag="NBL02_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCD2570761.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(45423..46397)
                     /locus_tag="NBL02_000044"
     CDS             complement(45423..46397)
                     /locus_tag="NBL02_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HCD2570762.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(46421..46465)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            46680..47648
                     /locus_tag="NBL02_000045"
     CDS             46680..47648
                     /locus_tag="NBL02_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD2570763.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            47761..48039
                     /locus_tag="NBL02_000046"
     CDS             47761..48039
                     /locus_tag="NBL02_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCD2570764.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            48157..48393
                     /gene="secG"
                     /locus_tag="NBL02_000047"
     CDS             48157..48393
                     /gene="secG"
                     /locus_tag="NBL02_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCD2570765.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            48496..49251
                     /locus_tag="NBL02_000048"
     CDS             48496..49251
                     /locus_tag="NBL02_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCD2570766.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            49254..51611
                     /gene="rnr"
                     /locus_tag="NBL02_000049"
     CDS             49254..51611
                     /gene="rnr"
                     /locus_tag="NBL02_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCD2570767.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWATELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            51633..52097
                     /gene="smpB"
                     /locus_tag="NBL02_000050"
     CDS             51633..52097
                     /gene="smpB"
                     /locus_tag="NBL02_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCD2570768.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
BASE COUNT        17324 a         9121 c        11123 g        14548 t
ORIGIN      
        1 atttgttatt ctctcatgat tatcagggta tatttttatt tattatgtcg gtgttccagg
       61 aagtgtttta atttaataac gaagaaatta attgtttgta gaatgaagga aactatttat
      121 tgggtagatg aatcaccacg aatagtcaaa aggagatttc aggatatgcc aaaaatgaaa
      181 aaaatcattt tagcagtaat gttattttct acggttatat cttttacagc cccttttttg
      241 cttacttaag ctgattttaa gttttggccc ttagtctgga tctacgaggt gagagtcaag
      301 aatgaaaaaa ataattggag cttttgtatt tgcaatcatt atcattatta tctgttttca
      361 aatagatgtt tttggtcaaa aaaagatagt tggacaaacg gtcagcagta cgaacagtac
      421 ggttacaaag acaaaggaat catttgatga tacagaacaa gagacattaa tcaaaatcgg
      481 aaaaaatgac cgtacactat atacagatga ctcgctaact accccacttg caactataaa
      541 cggcggagag ctggcggagt ttctttctga aacaaaaaat gcttatcaga tcaagacaaa
      601 tgatggctat actggatatt tagccctcga agatggaacc aaagtcacaa agaatataca
      661 aacaaaaccg gaagagctat ctgatgcagt gattgtatta gatcctgggc atggcggaaa
      721 tgatactggg gcattaagca atgatgaaca aacagaagaa aaagatataa cattaagtac
      781 agccataaaa gtaaaaaagg cgctagagga tgctggagca acggtttatt tgacccatac
      841 tacagatgaa ttagtacagc ttggagatat atgcgactac agtgaagaaa aaaaggcaga
      901 tgtctttatc agtttacacg cagactctac ggaatatgcg aatgaagcaa ctggaatcac
      961 gacatattat tactatggac aagaggaaac attagcacag acaattgctg actccttcac
     1021 tgatctgcct ctggattcaa gaggaatctc cactgggaac tatcaagttc ttcgagaaaa
     1081 tcttcaacct tctattttaa tcgagatggg ttatatgaat aatgatagtg atttaaagga
     1141 attagtaaca gacagttatc agcagcagat tgctgcaagt ttgacacaag cattgaccac
     1201 ttattttcag taataaataa gcccagccaa acaaaaatag ctcctcatat ttttcgtttg
     1261 gccgggctta ttatcgcaga tgcaagcttt taaacaccgt ttaaggaaga ggtcgtgagc
     1321 ctgccgttca actctgagtc acaaggagca atttcaaaaa acagcttctc atgtttgatg
     1381 actattgtga cttgtgaccg aggagttggc atgagaacac cgtttaagaa aaaggttgtg
     1441 aaatcaacgt tttgttctga gtattagaga aacaaacgga aaaatagttc ctcatatttt
     1501 gccgtttgtt gagctaatac cgaggatcag atgattgaac accgtttatt cggaacaagg
     1561 gactgtgaca tgacttttgt cacagttcca ttattttttt agtagcttca aagctttttc
     1621 tgtattggca aaatgaagtt tggcatattt ttcaagcata ggcatgccgc ctttctccaa
     1681 tagttttgcc gctacttggt cagatttcgt tccagctcct gacggaaggg tgattccagc
     1741 ttcagctgat gctttgtcta actcttccaa aaagctggca cgactgatga tagaagcagc
     1801 agcaacagct aaatgatatt gttctccttt agtaacgaag tacagttttt cttgtaattg
     1861 atttttttct aaacgcgcat attttttaaa atttgcttcc ggcgtaaact gatcgatcaa
     1921 aatcgcttct ggtttttctg gctcgatttt ttttagcagc aaatggatcg cttgattgtg
     1981 caaagcgact ttcatccgaa cggcattgta ttttggttgg atctcattgt atttttctgg
     2041 agataagaca agcagtttat aggggatggt tttttttaag atgtcagcaa gctggatgat
     2101 ttggctatct tttaactctt ttgaatctct aacaccaagt ttacgtaatt ctgcaagctg
     2161 atcacgtttc acataagcag cacatactgt tagcggaccg aaatagctgc catttccgac
     2221 ctcatcacta cctaaaacgg ataattgact gatatttttt ggcaaagaag aggctgtttt
     2281 tgctggcttg gcttgtttat cctgtgcctc cccccattta tcagcttcta ttgctgcatt
     2341 cgtaccttga aacatcactt ttccggatgt ataagcggta atggtcgtcc cgtctttttt
     2401 tgcagcaaag ctagtataag gaatctgttt atttaacaaa taagagcgat agtatgcttt
     2461 catttgacta agttggtcgt ttgttgcttt gataatgacg ttgctcataa aattctcctc
     2521 gtttttatac ataaaactat accgcaactc acgacttttc gttttctaag agattgcgag
     2581 acatagcatt tttttaaagt ttatcatata atagttaagt atgtgaaaaa tatttgtgga
     2641 gaacggtgaa agaaccaact tcatggtaca ctaaataaaa ctttcatcaa ataggaggaa
     2701 atgattcgta tggcgcacga aaaaacacgc tacaaagcgg tgattgccaa ccaaacatat
     2761 actattatcg gacgggaaac caagcatcat atggatatcg taacgaagct gatcaatgaa
     2821 cagttagcag aattgaaaca attatcacca caaatggata atgaacaagc agcaatcttg
     2881 atggctgtca atgcgctttc ggatcaattg aagaaacaag aaagaatatt ggagctggaa
     2941 gaagaaacag cagaattaaa gaaaaaaatg atcaaattta cagagcttga aaatcgtgtg
     3001 aaacgcattg aagcaatcga gaacgaagct cgggaagtat tgaaagaaaa tggtcaagca
     3061 gatcatacca ttaaaaatca tgtggaagca caacagattt taaacgagaa acgaaaagga
     3121 caaattaaac aaaaatcttc taattaagat ccttttatca aaataagaaa aatttaaaaa
     3181 agttagaaga gaaaggaacg tagcatgctg agtttgctga ttttattttt attgctaatt
     3241 gcgttttttt ctggagcacg tcgcgggttt gctttgcaaa tcatattcgc gattggatat
     3301 gtattgtcat ttattgctgc ccagcatttt tacaaaccat tagcaagtca tttggaatta
     3361 tacattccat acccagctgt caccccaaca tcaaagttag cattttttga tcaggtattt
     3421 gcctttcacc tagatgaagc tttttatgca ggaactgctt ttctgattat tttgctaatt
     3481 ggctggctgc ttacccgatt tgtgggggtt tttgtccatg gattgacgta tgtgccgatc
     3541 ttgagacagg tagactggat tgcaggaggc atcctgtcac taatcatggc ttacgttact
     3601 atatttttga ttttgcagtt acttgccttt gttccattag atgtggtcca aaatcaattc
     3661 ggcgagcata gtttcgctcg ttttatcgtt gagagaacgc catttcttac caacaagatg
     3721 tatgagcttt gggtaacgaa tattttgaat taaggaaaat agtgctggaa aatatttcgt
     3781 tttttgtaca gagtagttta tttaagaagg ctgcgtagcg tatgtccggc acagctcttt
     3841 tttcatagtg aaagataagg tgataaaatg aataaccgaa tccttgaaac attggaattt
     3901 gaaaaagtca aacagatggt cagacaattt gtagtgactg cacaaggaaa agaagaactg
     3961 gcagaattgg taccagtgag tgagaaacaa acgatcacaa actggcttca agaaactgag
     4021 gatggattga aagtgcaacg tctgcgaggc gggatcccta tacctaaaat tgaaaatatc
     4081 cgtccccata tgaaacggat tgagatcgga gcagacttga atggagtgga gcttgcacaa
     4141 gtttccagag tattgtctac gacaagcgaa ttgaatcgtt tcattgatga tctttctgac
     4201 agtgaaatcg aatttgcacg tttgtatatg tgggcagatc agttagtcac gatccctgtt
     4261 ttgagccgcc gtttaaagga agcaatcgat gaagacggac gcgtgactga tgacgcttct
     4321 cctgagttaa agagcataag acagaacatt cggcgaagtg aacaagctgt tcgtgaacag
     4381 ttagatggga tcgtccgtgg gaaaaatgca aagtatttaa gtgatgcgat catcaccatg
     4441 cgaaatgacc gctacgttat cccagtcaaa caagaatacc gcggtgtatt tggcggtgta
     4501 gtccacgatc aaagtgcttc aggtcagact ttatttattg aaccaaaaca agtggtggat
     4561 ttgaataatc gtttgcgtca ataccaaatc gctgaacgaa atgagattca acgtatttta
     4621 agtgaacttt ctgctgaact tgttccccat cgtcaagaaa tcatccacaa tacttatgtg
     4681 attggtaaaa tggatctgat gaatgcaaaa gcacgatttg gtaaagaagt aaaagccatc
     4741 gtgccgggaa tcagtgaaga caatcatgtc gtcttgaaac aagcaagaca tccactgatc
     4801 gatcaggaaa aagtagtttc taatgacatc acaatcggaa aagactatca agccatcgtc
     4861 attacaggac ctaacacagg tgggaaaacg atcacgttga agactttagg tctgttacaa
     4921 ctgatggggc aagcaggatt accgattcct gcaggtgaag aaagtcaaat cggtattttt
     4981 gaggaagtat ttgcggatat aggagacgaa cagtcgattg agcaaagtct atcgaccttt
     5041 tcttctcata tgacgaacac tgtagatatt ttatcaaaag tcaatgaaaa aagtctggta
     5101 ttatttgacg agctcggtgc aggaacagat ccgcaagaag gtgctgctct agcaatcgca
     5161 atcttagatg atcttggtaa aaagtctgct tatgtgatgg caacaacgca ttatcctgaa
     5221 ttgaaagttt atggatataa tcgggcaaat acgattaatg ccagcatgga gtttgatgtg
     5281 gatacattaa gtccaacgta tcgtttgctg attggcgttc ctggacgaag caatgcgttt
     5341 gaaatctcta gccgattagg tcttgataca gaagttattg atgaagcgaa gcagttgatg
     5401 aatgatgaga gccaagattt aaacgaaatg atcacagatt tagagaatcg ccggaaaatg
     5461 gcagagaccg aatatctgga aatgcgtcat tttgtttctg aggcacaaga acttcacgat
     5521 gatttgaaag aagcttacag ctatttcttt gaagaacgtg aaaaagaaat ggaaaaagca
     5581 aagaaaaaag caaatgaagt cgtgtcagag gcggaagaaa aagcagaaaa aatcattgct
     5641 gatattcgta aaatgcaaca gcagattggg caaggaaatg tgaaagagca tcagttgatc
     5701 gatgcaaaga cccaattagc aaatctacat caagaagaaa ctttgaagaa aaataaagta
     5761 ttgaagaagg cgaaagaaca aaaaacgttg aaaccgggag acgaagtgtt ggttacgact
     5821 tacggccaaa gagggacgct tttacgtaaa aacgggaatc agtggcaagt agaaatcggt
     5881 atattaaaaa tgaatgtgtc cgaagacgag ctgacgcctg ttgctcctca aaaagagccg
     5941 acacaacgag ttatccatgc tgtacgatct gaatctagta gtcacgtgcc gaatcaattg
     6001 gaccttcgag gaaaacgata cgaagaagcg ctaagtgagg tagatcaata tttggattct
     6061 gccattctag ctggttatcc tcaagtgact attgtccacg gaaaaggaac aggcgcactt
     6121 cgcaagggaa ttactgatta tcttaaaaac catcggagcg tcaaaagctt tgagtttgca
     6181 ccggcaaacc aaggcggaaa tggtgcgaca attgttaaat ttaagtaaag cttactaaag
     6241 gtaagcagat ggttcgctat ccaagtatag aaatgctata ctagtcatag attttaaaaa
     6301 tggaggtctt tgatatgtca caagtaatta cagataagac atttaatgaa gaaacagata
     6361 aaggacttgt attgattgat ttttgggcaa cttggtgtgg tccatgtcgt atgcaagcac
     6421 cgattttgga tcagttggaa caagaatatg atgaagaaga attcagaatt gctaaaatgg
     6481 atgtggatga aaatccagaa actcctcaac aattcggtat catgagcatt ccaacattaa
     6541 tgttgaaaaa agatggtcaa gtagtagaaa aagctgttgg tgttcattca aaagaacaac
     6601 tacgtcaaat gatcgatcaa tatttataaa aacttacagc aaaaataagc tgagggtggg
     6661 actgaatcca gttccgtcct cggttttttt gcggttgagt taaggttatt caaattaata
     6721 ttttttgagt ataattacgt tgtcaggctg gattgttttt ttgacagaca tgctataata
     6781 actttaattg aatatgaagg ggagtaccag atgaacgaac gaatcaaaaa caaattagct
     6841 ttacttcctg atcagcccgg ctgttacttg atgaaagata aaaatggaac gatcatttat
     6901 gtcggcaaag ccaaaatatt aaaaaatcgt gtccgctcat attttactgg tagccataat
     6961 acgaaaacgg aacggcttgt tagtgaaatc gaagactttg aatacatcgt tacagaatcg
     7021 aatatcgaag cattgctctt agagatcaat ctgataaaga aaaatgatcc taaatacaac
     7081 attatgttaa aggatgataa aacctatcct ttccttaaga taacgaatga aaaatatccc
     7141 cgtttggtga ttactcgtaa ggtactaaaa gacaaagcac tgtattttgg accatatccc
     7201 gatgtcggtg cggcgaatga gacaaagaag atcctcgatc gattatttcc tctgagaaaa
     7261 tgcaaaccta gtcagacgaa ggagccttgt ctttactatc atttgggaca atgcctgtgt
     7321 ccttattatt tcgacgtaga tccgaaagtc tatacgaaaa tcgttgatga agtgaaaaac
     7381 tttttaaacg gaggacacga aaaaatcgag tcagagatcc atcagaaaat ggaaaaagct
     7441 gctgaaaaca tggagtttga aaaagctgca gaataccgag accagatccg tgccattgag
     7501 acaatcatga ctcgccaaaa aatgacaaat actgatttac tggatcgaga tgtatttggt
     7561 tatgcgattg ataaaggatg gatgtgtgtc caagtgtttt tcgtaagaca aggaaagcta
     7621 attgaacgag atgtttcaat gtttcctttc tataacgaag cagaagaaga tttcttgact
     7681 tacctcggtc agttttatca ggaaaatgag catttcattc ctaaagaagt cttgattcct
     7741 gatacgatcg acaagcccag tgtagaagct ttgttagcta cgaaagtgct gcaaccaaaa
     7801 cgaggagaaa agaaaaaatt agttgaattg gcgagtaaga atgcctcagt tgctttgaat
     7861 gaacggtttg atctgattgc cagaaaacag gaacggacga tcggagcagt tgaacgttta
     7921 ggaaacgcga tgaatatccc gaccccaata cgtatcgagt cttttgataa ctctaatatc
     7981 atggggacga atcctgtggc tgcgatggtc gtattcattg atggaaaacc ggcaagaaaa
     8041 gaatatcgga aatataaaat aaaaacagtc aacggaccgg atgattatgc atcaatgaga
     8101 gaagtcatct atcgcagata ttcgcgagtg attcgcgaag ggttgccttt acctgatttg
     8161 atcttgatcg atggaggaaa aggacaagta gatgtggcaa aagatgtctt agaaaatcaa
     8221 ttaggaatcg atattcctat tgctggcatg gtaaaaaacg ataaacataa aacaaatgag
     8281 ttgctattcg gtacagattt ggctgtagta ccgcttgagc gaaattcacc agagtttttc
     8341 ttactccagc gaatccaaga tgaagtccat cgattcgcaa tcactttcca tcggcagcta
     8401 cgcagtaaaa acagttttgc ttcccgatta gatgaaattg aagggttagg accaaaacga
     8461 aaaaaaatgc tgcttaaaga gttcaaatcg ttgaaaaata tcacagcagc cacgacagaa
     8521 gaattacaag aaatcggact gccgaaaaat gtcgcacaaa acgtatttga taaattacat
     8581 caggaatgag gaaagggcac tttacaaaga aagtaaagtg tcctatcatt ttatgtacaa
     8641 caaaaagagc gtttacgttt tgttggtaaa taataaatcg cttacgtctg gtgtctaagg
     8701 atatatgtga tttcttgaag aaggagtgaa gaaatgttag aagtcaagga tcttgttaaa
     8761 acttttggga actacacagc tgtcgatcat gtctctttcc aaatccccga tggaaaaatc
     8821 atggggctaa ttggccaaaa cggggctggg aagacaacga cttttcgttt gatcttggat
     8881 tttttgacac aagaccaagg agaagtgtta tggaatggcc atcatcttgg ggagaaagat
     8941 tatgatgtca tcggttatct tccggaggaa cggggacttt atccgaaagt aacgattcaa
     9001 gaccaactgc tttattttgc agaacttaga gggaaatcaa gaaaagagat tgaaccaaaa
     9061 atcgactttt ggatggaaaa atttcaggta aaagggaaaa agacggataa agtaaaatct
     9121 ttgtctaaag gaaatcagca aaaagtccag ctgatcgcca cgttaatcca tgaacctaaa
     9181 ttgatcattt tggatgagcc attcagcggg ttagatccgg taaatgctga attgctaaaa
     9241 gatggaatat tggaattgaa aaaacatggt tcatgtgtca ttttttctag ccacaatatg
     9301 gataatgtag aaaaaatctg cgatcatttg atcatgttgc gaaacggaaa aatggttttg
     9361 aatggaaaag tacatgagat tcgtgaatca tttggccgta cgaagttatt cttggaatct
     9421 ggtttaagcc aacaggaagt agaagaaatc gaaggcgtga aaaaagtgat ccttcatgaa
     9481 gacaatacgt tagagattac cttagaagat cctgaagctg ggaaagaaat ctttactcgt
     9541 gcgactcagt tcggctacat tcctatgttt aaccagcaac cgccaacttt ggaagagatt
     9601 ttcaaattga aagcaggtga aatcgatgcg taagttttgg attattacca aagatgtcta
     9661 tttaaaaaat gttaagtcga tttctttctt gatcatgata ttagtccctt ttatcatgct
     9721 gggtatcatt tatgtagcag gaaactttgc tcaacaaaat agtgatgtca ataaaatcgg
     9781 cgtattggct gataatgagc aaatcagtca gcaaatgagc caagtcaaaa cagatgattt
     9841 tgagttcaaa gcagttgatt ctgaagaaaa agcacaaaaa gagctttcgg aagaaaaaat
     9901 cgatgcgtat ttagtagtct caactgagaa tgataagata aatgggatgc tttatagcga
     9961 aaattcatta ggacaaagta cacaactact gatacaacaa caactgactg gcttgcaatc
    10021 gatgcttcga gcgagtcaat taggcatttc accagaagaa gtagctagct tgagccaaca
    10081 agcaagtttg actcgacaaa aagtcaattt tgattcaaaa ggaaaaatga cagtcggaga
    10141 agataactcg acagtccaat acgtcgtaag ttatgtagca acgattgtct tgttcattat
    10201 tattttgacg tatgcgcaga tcattgcaca agagatcgct tctgaaaaag gcacgcggat
    10261 catggaagtg atcctgtcca gtacgactgc acaaaagcat ttttacggaa aattaacagg
    10321 tgttttactt gttgcattaa cgcaaatggc attatacggt attatttttg gtataggata
    10381 cagtcaattc aaagatatgg aaatggtcaa aaatatatta gacggtatct cattggataa
    10441 cttgattgga ccattcttgt ggtattctct tctattcatg atctttggca ttttgatttt
    10501 ttctgtttta gcagctttat gtggctcttt ggtcaacaaa gcagaagata cagcaaaagc
    10561 aattttgcca gtgacttatc tatctctagg cggatacatg cttggattga tactaggagc
    10621 ttcagatcca aacaatatcg ttattcggat cacttcgtat attccattct tatcttcata
    10681 tattatgccg attcgcttag caaatgagac agtcggtatg ggtggagcat tgatttcctt
    10741 gttggtactt gtagccgtaa cattcttatt gatgatcttg tctgcaaaca tgtacaaatc
    10801 gaatgtactc gtttatagcg aaggcggatt atggagttca ttgaaacaat cgatttcaat
    10861 tatgaggaat gaacgaaaaa aagcataaaa caatcctaaa atacagaagg aagctggaca
    10921 tctattgttc ccagcttctt tttgtataga cctattttat ttattttact atattggata
    10981 tatattcatt gaaaatagtg tataacaact ttataatatt gagtatacca atttttggag
    11041 gcgttttaat ggaccgttat ttagatttac tttcagaaaa attcccctca acggaggatg
    11101 tcatcacgga aatcatcaat ctcgaagcaa ttttgcagct tccaaaagga acagagttat
    11161 ttttgagtga tatacatggc gaatttcctg cttttgatca tattctgcgt atcggttcag
    11221 gaaatcttaa agaaaagatc aaagaccttt tttctgaaca aatgacggaa acggatatca
    11281 gccaattcac tatttttacg gcttatccag aatatgcatt aacgactgat tggtacaaag
    11341 aacaagataa atcgcagctg atccatcatt tgatcgatct tttacggttt acgacagtca
    11401 aatatacccg gtcaaaagtt cgaaaaggat tgccgaaaga atatagctat atcattgagg
    11461 aactcttgta tatcgatgac cgtatcaatg gaaaaaaaga ctatgtaaaa aaaatcattg
    11521 aacaacttgt tttgatgaag gaagaagaaa aatttttaac gaaacttgca cagacgatcc
    11581 aaaaatctgt catcgatcat ttgcatatcg ttggagatat ttttgaccga ggaactcaag
    11641 cagccaaagt gatggatcga atcatggatt tttcttctgt agatatccaa tggggaaatc
    11701 atgatatttt gtggattggt gcgtattgcg gctctgaggc atgtttactg acacttttac
    11761 ggattgcagc acgctataat tatttatttg aattagagaa agaatatggg ttgaatttac
    11821 gtccattgtt ttcttttgct catcaaactt atcagaagaa tccagtattt gcgcctaaaa
    11881 acagtaaaaa tctatccgaa agagagcaag aagaattaga aaaaatccat caggcacttt
    11941 ctatccttca attcaaatca gaacatcagt tattggatcg tcgacctgaa tttaaaatgg
    12001 acgatcgtaa attgctggaa aaaatcgact atgaagcatc aacgattgat ttagaaggtc
    12061 agctctatga gttgaaagga acttgttttc aaacgatcca gccagatgat ccaaaaaaat
    12121 tcttggaaga ggaacaaaaa gtgattgata gtttgatgta ttcattccag cactctttga
    12181 gaatgcaaaa acatatgacg tttttgatcg aaaaaggggg aatgtatctc acgaacaatc
    12241 agcatgtgct gtttcatggc tgtatacctg tagacgagaa gggacagctc ttagcatttg
    12301 aattagacca atcttatcgc ggaaaagaac tgctgggctt ttttgaaaaa cagataactg
    12361 aaagcgcaaa agatctgact gcaaaagaag atttagcaac cgatttgatt tggtatgcgt
    12421 ggagtgggcc attttcgcct ttattcggca agagtaaaat ggcgactttt gaacgttatt
    12481 ttctcacaga gcctcatacg cacgtcgaga aaagcaatcc ttattatcac ttgcgagatg
    12541 aagagtggtt cagtgaaaaa attttagcag aattcgacac aaaagaagaa gggtcagcaa
    12601 tcattaatgg gcacacgcca gtaaaagtaa aaaaagggga gtcaccgatc aaagcaaatg
    12661 gaaaagtatt tgtcattgac ggaggtttat ccaaagctta tcaaaaaacg acaggaattg
    12721 ccggttattc actattatgc aattcttatg gtttccaaat cgtgacacat tatccgtttt
    12781 tgcctattga acaacaattt gcaaaaaaat ccgaccaaac aaatgtgaaa aaagtaatcg
    12841 aacagcaact accacgcaaa ctcaatcgtg atacaacaaa aggtatggaa ctgcagcagc
    12901 agattaccca gctcaaaagg ctgttggact atcgaaagga cgattgagaa gaattccagt
    12961 gaacattgtt ccatagactg gagactgaga caaaagtcgt gtcgcagtct ctttttccgg
    13021 ataaacggtg ttcttctgcc aactcctcgg tcacaagtca cactattcaa caaacatgaa
    13081 aagctgtttt ttgaaattgc tccttgtgac tcagagttga acggcaggct cacgacctct
    13141 tggtataaac ggtgttcaaa agccagtttc tcggtattaa gccaaattta tgcaaaaatg
    13201 tgaaaagcca ttttcacaaa atttttctta atactcgaaa ctaaacagct ttttcacaac
    13261 cccctcagaa agtctattaa aattcattca caaagaaatc gaataagcgc tgttcagatg
    13321 gatgtacacg gtgggtaagt tccggatgcc attgaacacc gaggatttta tgcatatcat
    13381 ctttgctttg aaccgcttca acgagtccat cgttagaaaa agcgatggct tttaataaag
    13441 gtgaaagatc tttgactgcc tgatgatggt aagaattcac aaaatatgga tcggggagaa
    13501 ggttgcttaa caaactgtct gaaacaatct tgacttcgtg agtcgtaaat tgcggggcag
    13561 tgggctgttg ttcatgtttt actgaccact ttggatataa tgaaagatct tgataaagtg
    13621 taccttccaa aacgacattc aatagttgga gtccccggca gactgcaaaa attggttttc
    13681 tttgtttcaa tgcttccaag atcagtgcct gttcaaattg gtctcgttga atatttgttt
    13741 ccgttagctt tggatgagga tcttccatgt aaagttgtgg actgacatct tgaccacctg
    13801 caagaataag tttgtcgatt tgtccaatca gttgaggagc tgaatctgga gacatgactg
    13861 gcagaataag aggcaatcct tgagcatcta aaactgcatc cacgaatcct tgcggtgtat
    13921 agctcacttg atttccttgg aaagtatctg ttgctcgaat cagttgattt ccggcaatgc
    13981 cgatgattgg tttcatttgt attcccccta aagtagactt attgtaacaa ggatttaaaa
    14041 aaaattaaac tatgtaagac ttgcccctat acttatctgt tcttgctgtg ttactatctg
    14101 taaagagaaa tttcgtgtga aattcatgac gatagttgaa aatattgctt aatttgttat
    14161 aataaaaagt aagcagaaat aaaaaggttg ggcgtaagct tcaaggatct agttcttcaa
    14221 ggggtgagaa ggacatttta ttgaaggggc ctgcctatac taagttatct ttagaggtac
    14281 atactttcaa tgaaaaaaga aagcatgtac ctctttttta aatcgaaagg aagtgaagca
    14341 agtgttagaa gctttggaac aaatccgaaa agcagaagaa gaaaatgaaa agcgtgctca
    14401 aacactgcgg cagcagctga agatctatgc aacagaaaaa gagcaggaat tggaaacgat
    14461 ccaaacaaac tatcgacaac aactaaaaca gttggtggaa gaaaaagaac aatttgaaat
    14521 acaagaatta gaaaaagaaa aaatgaaatt gaaagcagaa gcagacaagg tgcaagcaac
    14581 tttgaaagat caatatcaaa agcatcagag tcaagcgatc ggtgcgatca ttgaaagggt
    14641 gaaagaaact tatggccgtc actaagatgg aaaaagtgac cctcatttca gataaaagaa
    14701 atcaggagag gattttacaa gcagtacaag gaatccaaaa cgttgaaatc cgggatctgt
    14761 atcaaaatag aacaaacaac cagtgggtgg agaagtattt tccagatacc acgattattg
    14821 ataaagaagc aaaattaagc gctttaggta atcggttgaa agacatccaa gaagctatcc
    14881 aattcataca acaccacggc gatagccgtc aaaagaaact ccatttgaaa cgtcaagaac
    14941 tttccttaca agatttggaa gccaattatt ccgaagaaaa tttcataaat aaattgaaag
    15001 aagtccttga tttaaaagaa aagtgggaac aactaactga agaacgtgat cgtttagaag
    15061 aagaggaaga atggcttctt cattggcaac agctggatat tgctccaagt atgtatgcaa
    15121 gtaagacgac catttttgag atggggactg tgagtgcagc aaattttgaa agtttcaagg
    15181 aagaactgag taagcttgat gacgtttata tggaagaaat agattatacg ccaaaacaag
    15241 tttatctttc gtatgttgct ttaaaaaaat cagttgctgt tatagaagaa attgctggga
    15301 aatacgggtt caataaagaa acgtatcctt atgaacaaaa tcctaaagaa aaactgcaag
    15361 agaccaagca gcaattatcc attgttcacg agcagcaaaa gaagataacg acagcgttag
    15421 gtcagtgcag tggctatatc caagaatttg aatgggtaga agaagtcaca cttgccgttg
    15481 cagaacgaga aaaaataaaa gaacggttta tccatgcgac ttatctgatc gttttgcaag
    15541 gttgggtaga tgcagaagaa aaacagaatt tgctgcatgt gttaaacgaa accgtatcag
    15601 aagaagatat atacgtcagt ttcgaaacac caaactcaga agagattcag caggaagtcc
    15661 ctactaaatt gaaaaaccat ccgcttgtcg aaccttttga attgctgact gaaatgtaca
    15721 gtctgccaaa atatgaagaa gtagatccaa caccttggat gacaccattt tatcttgtct
    15781 tcttcgggat gatggtcgca gatgttggtt atggtttgct gatgctcatt gggacaatac
    15841 tagctcaaaa actacttgtt ctaccacgcg ggatgaaacg tttcgtaaaa ttctttgaga
    15901 ttctctcgat accatcgatc atctggggat tgatctacag ttcatttttc ggacagtcat
    15961 taccaaaaga actgtttggc atccgcttgc ctttcccaat cctatctacg acggatgacg
    16021 tcaatacgat tttgatttta tccgtgattt tcggtctgat acagattttg gttggattat
    16081 ttgtggcagc aaaggaaaat atcaggagaa aagattactt gagtgctgtc agtgatggat
    16141 ttgcctggca agggatccta attgggatcg ttattgcgtt ggttggtgcc atgttgataa
    16201 aaaatacagc atttgtttac cttggcgctg ggctatcgat cattgctgcc ttatgtatct
    16261 tgatcgttcc gattatccaa acgccgtcta aagtcaaagg tgctgccaaa ggtgcgtata
    16321 atctttacgg actgaccggc tatatcggtg atttagtcag ttatacacgt ttgatggcat
    16381 tgggtatttc aggcggaagt attggagcgg cattcaatat gctagttgct ttcatgccgc
    16441 cagctgctcg attcagtgta gggatccttt tgatcatcgc tttacacgca ctgaatatgt
    16501 ttttgacact gctaagtgcc tatgtccatg gcgcacgatt gcagtatgtc gaattttttg
    16561 ggaagtttta tacaggaggc ggacgtgcct ttgatccgct gaaaacggcg gaaaaatacg
    16621 tcaacatcaa tcacaagaaa aaaaacaata aataattgga ggatttttat ataatgatgg
    16681 attacttaat tagtcaaaat ggtggaatgg tatttgcggt attagcgatg gcaacagcga
    16741 cgattttttc agggatcggt tcagctaaag gtgttggaat gacaggtgag gcagcagcag
    16801 cattgacaac aagtcaaccc gaaaaattcg ggcaggctct gattctgcaa ttgcttccag
    16861 gcacgcaagg tttgtatgga ttcgttattg cctttttgat ttttatcaac ttaggaaatg
    16921 acatgtcagt tgtacaagga ctgaactttc taggtgcttc tctgccgatt gcttttacag
    16981 gcttgttttc aggtattgcc caaggaaaag tcgcagcggc aggtattcaa attttagcga
    17041 aaaaacctga acatgctaca aaaggaatca tttttgctgc gatggttgaa acatacgcga
    17101 ttttaggttt cgttatttcc ttcctattag tgttgaatgc ctaagcataa aggaggaaaa
    17161 gcagatgaac gccattgaaa aaatcatctc tcagatgaac gaagcagcag agcaggaaag
    17221 agcggcgtta gaacaagaag agcgaatgaa gatcgatcaa aactttgaac aaaaacgtac
    17281 gcaagttgaa acagaacatc aaaaacaaaa agaaaaacaa atcgaattac tcgaaaaaaa
    17341 ataccgtcag ctaaggaatc gccagcaagt agaagtacga caagaaaatt tgaatgcgaa
    17401 gcaggaattt ttaagacgat tgtttgcaga tgcggtaact gaaatggaaa attgggatga
    17461 atctgaacaa atccagttta tcaaaaatgc attgtattca ttacctttga caggaaaagt
    17521 agcattcatt gctggagaaa aatcagcagc ctatctttct cagacgttgt tggatgaatg
    17581 gaacaatgaa cttcccttta tgatggtttt aagtgatgaa actgttgctg atcaagcggg
    17641 ttttctgata aacgatcaag gagttcagta taattttctt ttcagcagcc ttgtacaaga
    17701 tatccaagga acgatgagtt ttgagattgc caatcaatta ttcgaataag gagggatcag
    17761 tgtatggaat accacgaact aaatcccttg attcgcggaa gagagcttga acttttgtcc
    17821 aatgagacat ttgaacggat gattcaaaca aattctgtcg aagcacttgg tgaaatattg
    17881 aaatccacga tttacagtcc atatatttat gacggatatg aatatgactt cgaacagaat
    17941 cttttaaagg agcagagccg tctatttcgc tggttaaagg aatcagcacc ggagcctgaa
    18001 atcgtttgga tctatacgat gcgttatact ttccataatc tgaaggtttt gacaaaagct
    18061 gagatgacaa atcaaaatct ggaccatttg tatatcgacg atggatttta ttcattagaa
    18121 accttgaagg atgccattca tacgcaagtt tcaaatgaac ttcctgaaag cattatggac
    18181 agtatacgag aagtacgtga ttattttgaa gagtctacca ttcttcaggg aattgacgtg
    18241 atttatgatc gacagttttt gacagagcag cgacggcttg ccgataagct aggatatcaa
    18301 gaattgctga cagaagtcat tgcttttatt gacttgacca atatcacaac gatggctcga
    18361 ggaatattgc agaaaagaac atatgggttc atgacgacgg tactttccag ctcgggaagt
    18421 atcgaaaaag aagtattttt gaactttgta gataaagata tggaagatta cacgaatttt
    18481 ctattagaga cagattatgc tgattttctg cgccctgtca tccgtgaggg aaatattgat
    18541 ctcgtaaagc ttgaacagtt gaaagatgat tatctaagtt cattgtatca agaagcacaa
    18601 acccaggctt tcggaccact gccactacta gcctttttga acgcaaaaga ggtagaaagc
    18661 aaaaaccttc gtttactggt tatcggcaaa agaagccatt tcgaatcaga aacaatcaga
    18721 aagcgggtga gacaagtcta tgacgcataa gatcggtgta gttggcgaca aagattctgt
    18781 tttgccattc aaattgtttg gctttgatgt tcgttatggt acaaccaagc aaaccgttcg
    18841 tcaggcaatt gaagaaatgg caaagaatga gtatggcgtg atttatgtca cagagcaatg
    18901 tgcagcattg agcccagaaa cgatcgaacg ttacaaggaa caaatgatac ctgctatcgt
    18961 attgattccc aatcatcaag gatcattagg gatagggctg gaagaaattc aaaaaaacgt
    19021 agaaaaagca gtaggtcaaa atatcttata gtatgaaaag gagatagaaa ttttgcaaat
    19081 tggaaaaatc ataaaagtct ccggacctct tgtaatggct gagaacatgt ctgatgccag
    19141 tatccaagac atgtgtctag tgggagatct aggagttatc ggagaaatta tcgagatgcg
    19201 tggcgatgtg gcgtctattc aagtatatga agagacttct ggaattggtc caggcgaacc
    19261 ggttcgctct actggtgaag ctttatctgt tgaattagga ccagggatca tttcacaaat
    19321 gtttgacgga atccaacggc cattagatac ctttatggaa atcacacaaa gtaatttctt
    19381 agggcgcgga gttcaattgc ccgcattgga ccatgagaaa aaatggtggt tcgaaccaac
    19441 agtagaagcc ggagaaacgg tgagtgctgg agatgttatc gggatcgtag aagaaacaaa
    19501 agttataaaa cataaaatca tggtgccaaa tgggataaaa ggaacgatca aaacaatcga
    19561 aaaaggttca ttcactattg atgaaacagt atgtgtcatt gaaaccgaaa atggtgacaa
    19621 aaatttgacg atgcttcaaa aatggccagt aagacgtagt cggccaatca aagaaaaatt
    19681 aaacccggat gcacccatga tcactggaca gcgagtgatt gatacattct tcccagtgac
    19741 aaaaggagga gcagcagctg tacctggtcc ttttggtgca gggaaaacag tcgttcagca
    19801 tcaaattgca aaatgggccg atgtggacat ggttgtctat gtgggatgtg gcgagcgtgg
    19861 aaatgagatg acggatgttt taaatgaatt ccctgaattg attgacccta atacaggaga
    19921 atcgttgatg gaacgtacgg tattgattgc taatacatca aacatgccag tagcagctcg
    19981 agaagcttct atctatacgg gaatcacaat tgcggaatat ttccgtgaca tgggttatga
    20041 cgttgctatc atggcagact ccacttcacg atgggcagaa gcacttcgtg agatgagcgg
    20101 acgtttggaa gaaatgcctg gtgatgaagg ttatccagct tatcttggtt cacggcttgc
    20161 ggaatactat gaacgatctg gaagagtcat tgcattagga tcagaaggtc gcgaaggaag
    20221 tataaccgca atcagtgctg tatccccgtc tggaggggat atttcggaac cagtcacaca
    20281 aaatacattg cgggtcgtaa aagtattctg gggtcttgat tccagtttag cccaaaaaag
    20341 acattttcct tcgatcgatt ggatacaaag ctattctctt tattcaacag aagtagggaa
    20401 gtatatggat catatccttc aagctgactg ggcatccatg gtcaccgagg gcatgcgtat
    20461 tttgcaggaa gaacagcagc tgaatgaaat cgtgcgtttg gtagggattg attccctttc
    20521 agataatgac cgtctgacat tagaagtggc aaaatccatt cgtgaagatt atttgcagca
    20581 aaatgctttt gatgatgtcg atacattcac ttcaagagag aaacaattca aaatgttgaa
    20641 gatgatcttg acctttggtg aagaaggacg ccgtgcgttg tcgttaggtg cttatttaag
    20701 tgagatcatg gacggtaccg tatcgattcg tgaaaggatc ggacgaagca aatatatccc
    20761 ggaaacagaa ttagaaaaac tagatcaaat tattggtgaa atcaagcaaa cgatgcaaac
    20821 gatcgtttca gaaggaggca tgagcaatga ttaaagaata tcgtacgatc ggtgaagtcg
    20881 tcggtccatt gatggcggtt gaaaaagttg ctggtgtcaa atatgaagaa ttaatcgaag
    20941 tccgtatgca aaatggcgag attcgtcgtg gacaagtatt ggaagtccaa gaagataaag
    21001 caatggtcca aatattcgaa ggaacaagtg gtatcaattt acgtgactct tctgtacgat
    21061 ttttaggcca tcctttagaa ctaggtgtat cggaagacat gatcggtcga gtattcgatg
    21121 gtttgggacg accgaaagat aacggaccag agatcctgcc tgaaaagcat ttggatatta
    21181 acggagaagt aatcaatcca gtagctcggg attatccaga tgagtttatc caaacaggta
    21241 tctctgcaat cgatcatttg aatacattag ttcgtgggca aaaattacca gtattttctg
    21301 gctctggtct tcctcataaa gaattagctg ctcagatcgc gcgacaagca acggtactcg
    21361 attcatcaga tgatttcgcc gtagtctttg ctgctattgg aatcactttt gaagaagctg
    21421 aatttttcat ggaagatttt cgtcaaacgg gcgcaatcga ccgttctgtc atgtttatga
    21481 atttagcaaa cgaccctgca atcgaacgta tcgctactcc tcgtatggct ttgactgctg
    21541 cagaatatct tgcttatgaa aagggaatgc atgttttggt tatcatgaca gacatgacca
    21601 actatgcaga ggcattgcga gagatttctg cagcacggcg agaagttcca ggaagaagag
    21661 gatatcctgg gtatctttac acgaacttag ctactttatt cgaacgagca ggaagaatcc
    21721 gcggactaaa aggatcggtt actcaaattc cgattctaac aatgccagaa gacgataaaa
    21781 ctcatccaat ccctgacttg actggttata ttacagaagg acagatcatc ttgactagag
    21841 agctttacaa gagtgggatt cagccaccaa ttgatgtact cccgtctctt tcgcggttaa
    21901 aagataaggg aactggaccc ggaaagacaa gagaagacca tgcagctacg atgaatcagc
    21961 tttttgcagc ctatgcacaa ggaaaacagg caaaagaatt agccgtagtt ttaggtgaat
    22021 ctgctttatc tgatgtagac aagatctatg cgaagtttgc tgagcggttt gaaaaagaat
    22081 atgtcaatca aggattctac acgaaccgtt cgattactga aacacttgat ctagggtggg
    22141 aactgctatc gatgttgcca agaaccgaat taaaacgaat caaagacgac atgctggaca
    22201 aatatctgcc agaagggaag tgagttctga tggcacgatt gaatgtgaat ccgacccgca
    22261 tggaattgac tcgattgaaa aaacagttga caactgccac aagagggcat aaattattaa
    22321 aagataagca agatgaactg atgcgtcaat tcatcctgtt gattcgaaag aacaaccagc
    22381 tgcgtcaaga aatggaagag tctatgacag aagcaatgtc tgattttgta ttggcaaatg
    22441 cttcgatcac ggaacctttc atcgaagaat tatttgtttt gccggcggaa aacgttgatt
    22501 tgtctatcgt agacaaaaat atcatgagtg taaaagtacc tgtgatgaat tttgaatatg
    22561 atgaacagct gacagacaca cctttggaat acgggtattt gaattccagt gccgaactgg
    22621 atcggtcaat cgatcggttt actaccctgc ttccaaaact tttggaactg acagaaatcg
    22681 aaaaaacttg tcaactgatg gcgagtgaaa tcgaaaagac acgtcggcga gtcaatgcat
    22741 tagaatatat gacgatccct caattagaag aaacgatcta ttatattcgt atgaagctgg
    22801 aagaaaacga gcgcgcagaa gtaacaaggt tgatcaaagt aaaaaatatg ggtaccactg
    22861 actaagtggt atcttttttt aaaagaggaa aacgatgatt aaacaaagca aacaaataaa
    22921 gaaacgtttg ccggctgttc agcttctcgc attaggattt tttctcctga ttttagttgg
    22981 cggcggccta cttaccctac cattttttag taatagtggg catgggacaa attttatcga
    23041 tgcgcttttc actgcaactt ctgctgtctg tgtaacggga ttgacaacat tgaatacagc
    23101 agagcattgg aacagtgctg gtcaattttt gatcatgctt ttgattgaga tcggcggtct
    23161 tggttttatg atggttccga ttctatgctt tgcattgata aagaagaaag tcagtctcag
    23221 cacacgtatt atattaaaag aagcgctgaa tctagaagaa gtatcaggtg tgatgaagct
    23281 catgctttac atcctaaaat tggcctttgt gatccaattg attggtacgg ttgctttatc
    23341 ttttgtattt gtgcctgaat ttggctggtt gaaaggtatc tggtacagtt tgttccatgc
    23401 aatctctagt ttttgtaatg cagggtttga tttgttagga gacagtttag ccaatcacca
    23461 gcaaaatgtt tatttgatca tggtggtatc tgctttgatc atttctggtg ggttaggctt
    23521 catcgtatgg cgtgatttac tggaatatca tcagaaaaag aagatgacga ttcattcaaa
    23581 aatcgcatta accgttacag gtaccctttt attaggcgga ttcgttgtgt tctttttcac
    23641 agagcgcaat ggggcaacgc tagtggaagg gaatatgttt caacgattgg caaacacctt
    23701 ttttatgagt gtgacaccta gaacggctgg atattattca gttgattacc tccatatgag
    23761 ccatgcaggt ctgattttaa cgatgttttt gatgtacatc ggcggaacat ctgggtcgac
    23821 ggccggcgga ttgaaaacaa ctacgttagg tgtgttgctg atccagatgc gttcgatgtt
    23881 gaaaggacgt acgagagcgg aagcatttgg ccgtaccatc cgacaaggcg cagtgttgcg
    23941 ggcacttact ttattttttg ttacgttgac gttgtgtgta gtcgccatca tgattttatc
    24001 ggtgactgaa acgataccta aaacttctgg catcgaatat attgcttttg aggtcttctc
    24061 tgcgtttggt acagtaggtc ttacgatggg attgacgcca gacttgactg caatagggaa
    24121 attgatcatt atagcgctta tgtacattgg acgagttggt gtattgactg tggtcttctc
    24181 tctgatgaca aaagcacaaa gaacacaatc aaaatacaaa tatccagaag aaagtgtcat
    24241 gattggataa tttcatcttc tgacagaaac ctagtgattt agtaaaataa gaaaataaaa
    24301 taatttttct tgcttcttgg atactttaag tgcaaaatgt tcgtttattt tttgaggcac
    24361 tacccgttgt tgaacctcgg gtagtgcctc ttttttattg aaaggaatca ataagatttg
    24421 attcctttat ttatcaatag gttataattc atattttttt attttttctt ttacgatact
    24481 tagtccagaa gctaaaattt caaggttatt agaggatagg gaaattcgaa cgaatgcatc
    24541 tggttgcgca cgtgtagtaa agcgtctgga atgataaagt cggataccgt tagcaagaaa
    24601 gtcattctca attatcttag ggtcaatctc aggatcaact ggaagaattc gatagtaagg
    24661 gacacgattc gttggttttt ctactttgaa aagagaatca aaaagctgat tggctctttt
    24721 tgtaagactt aatttagttt ctaagatcct atttgcttca tttgaatgga ggatttgagt
    24781 gatgacttcc acatctaatc cagaagtttt cacatttata ttaaaaattg cttgttctat
    24841 cttgctttgg acatgtactg gaactacaag ataagcaact cgcaatccag aacaaataaa
    24901 ttttgtcatt ccagctaagt agattgtttg gttaggtaat aaattttgga aaggaggaat
    24961 gatcgtatgt tgatagtaag ttgttaaaaa tgaatggata tcatcttcaa taacccatag
    25021 accttctttt tcaatcactt cggctaattg ctcccgtctt ttgtgagaga tttgaaaccc
    25081 tataggatta ttacaagaag gcataagaaa gacaccatga atcttctttt ttctacattc
    25141 ctgaatcaat atatcaggtc gcattccttg ctcgtcaaaa tcaattggaa cgatctctaa
    25201 atgatataac tgagccaatt caatgaaatt agcgtatgta tatcggtctg ttgctattct
    25261 atcacctgga gagaaaagtg cagaaagtgt gatagccaat ccattttgaa caccggaaac
    25321 aatagataga gattctaagg acggaatgac tccttgagtt tttaaccaat ttgctgcagt
    25381 agtcaattga aaatttgtgc ctttgggatc ttggtaatta agcaaaccaa caagatttgg
    25441 gttattacta gttgattgaa taaacgggac aacgaattta ttacattctt caaaagaact
    25501 aaccagacca aaatcaatga tatgttcggt tacttcattg gtagagatgg tgatggactc
    25561 aaagacattg ggagaaataa atgtgccact tccaatattc gtatacagca aacctttctc
    25621 aattccatat ttataagcct tgccaacggt agtaaaattt aaatctaaat aatcagctag
    25681 ctctctttgg gagggtaact gagtgttttt ctgtaattta ttctttaaaa tatcttgttc
    25741 taattgttga atcaacgatc gataataagg acgcttcaat ttttgtttgt ctggtcgcca
    25801 agttaacggg taattgtcat aagaattaat aggcattttc tccactcctt aaaattgtat
    25861 accatacaat tttaccattg attgtattga ttttagaagt taatcttttg tgaggaggga
    25921 attgtctatg aaaattatta attttgagga agtagtagct tcttttagtt ttgaagaagc
    25981 gattcaagta atgaaaaaat gtttttttga ttatgaaagt ggtaaaatca gccaaaatcc
    26041 tcgattggtg gaaattttac ctgacggaaa aggcaaaaat atgtttgcga tgatgcctgc
    26101 ttatttgggg gagaacagat attttggcac aaaagtcatt accgcttttc cggataatca
    26161 tcagtatcat ttaccttctc atttaggaga gattttcttg tttgatgctc aaaacggact
    26221 acccaaagca ttgataaacg caaatgcagt cacctggatt cgaacagctg ctgtatctgc
    26281 gctggcaaca gattacttag caaatccgca tgcagaaaca cttgctttaa ttggagctgg
    26341 tcaacaagca ttgtcgcatt tgactgcgat ttctaccatt cgtcccatca ctacagtgtt
    26401 tgtttacgat attttagaag aaagacgaaa tacttttatc gctaaagcta aaaaacagta
    26461 tcctaaaata acgtttataa atgcaatatc agtagaagat gcagtacgtc cagcggaaat
    26521 catttgtacg ttgacctcta gtaaagaggc atttcttcaa aaagagtggg taagggaaga
    26581 cgcacatatc aatgctgtgg gcacatttac tcctgataca agagagataa gtacatcgtt
    26641 gatggaaact tctcaaatct atgtagatga ttactcagct gccttaacag aaagcgggga
    26701 cctgcttata gccatggcag aaggagattt agaggaatct tgtattgttg gtacattagg
    26761 cgaattagtt tctgagaaaa aaacatttta taaaggagaa tcgaggccga cgattttcga
    26821 cgcagttggt ctagctattg aagatctttg ttgtgcagaa tatatttata acaaattaga
    26881 gaaaggggct ttaaaatgaa cttagattat tacgtagttg atgcatttgc agatgaggtg
    26941 ttcaaaggaa atccagcagc ggtttatgtg ctagaagaat ggctgccaga aggtacgatg
    27001 caaaaaattg ccattgaaaa caatttatct gaaacagcat ttacagtgaa gaaaaaccaa
    27061 gaatttgagc ttagatggtt tacgccagat cgagaaatcg atctttgtgg tcacgcgaca
    27121 ttagcaacag catttgtttt atttaactat tacaaaatac ctgatgaaac gattaaattt
    27181 tctactcaaa gcggtaatct atttgtgaca agaattaaag attattatta catggatttt
    27241 cctagtatta tgccgaaaaa agtgccgata ttgtctgaat atgaagaagc gattggggct
    27301 aagattaaag aagcctactt agcacgtgat ttatttttcg tattagaaga tgaaaaaacg
    27361 gtggctaaat tgcagccaga ttttacagct atcaaaaatt ttgaattagg aataggagtc
    27421 atcgtgacag cagaaagtgt gcaaaaagac tttgtttcta gaaccttctt tccaaaatta
    27481 acaatcaatg aagatccagt atgtggatca gcacattcta atcttattcc ttattgggca
    27541 gaaagaatga ataaaacaaa attgtcagct tatcaaatgt catcaagggg cggggatcta
    27601 gaatgtgaat taaaagaaga tcgtgtgata ataggaggaa aagcagtttt gttttctaaa
    27661 ggtacctcgt ttatttgatt tctttcaaat gaattaaaaa taaattggtg gagagtggca
    27721 accttatagc tacaatggtt gttaaaaaaa gacgcaaact ttttcaattt aatgaaaagt
    27781 ttgcgtctca ttttaattat tggtgtttgt tttttattca atcaagagta aaatgcccca
    27841 tactaactat cctcggatga cttcgatttt ataaccatca gggtcaatga caaagtagta
    27901 agaaggcgca gtgccaggta atccttttaa gtcagtaatc tcaagaccag cagctttatg
    27961 ttgctcgtgt aatttttcta gatcatcagc tgctatagcg atatgaccat agccgtctcc
    28021 tagatcatac gcaccatgat catagttgta agtcaactct aattcatagt catccccagg
    28081 aagtgtcaaa taaacaagtg taaatttgtt ttctggaaaa tcacggcggc ggctttcttc
    28141 aaaaccaaat gccttttgat aaaattcgat tgatgcttca agatctttca ctcgaacaca
    28201 tgtgtgagcc attttcatag attcatctct ccttttcctt ttactcaatc ataacaaaaa
    28261 gaagtaagga aatagttgat ttgtgcttgt tcatgagaaa tttctcatgt aaaatggata
    28321 gaaaataaaa ctgttaaggt ggaaactatt catggataaa ccaatttttg gtgaaaaaaa
    28381 gcaatttgct gattatcaaa cccgttatgc agcatatatt gtcatagaaa gagatcagca
    28441 gattgcagta atcgaggctc caaatggtgc tttttttctt cctggcgggg aaattgaagg
    28501 agaagagacg aaagaagaag cgattgtccg tgagctgata gaagaaatgg gaattgtggc
    28561 agaaatttct tcttatctgg gagaagctga tgagtatttt tactcgaatt atcgtgcaac
    28621 ttattattat aatccaggat atttctttgt tgctgattct tggaagcgta taggagaacc
    28681 aactgaaaag accaataaaa tttggtgggt gagccctcaa gaagcgcttg tgaaattgaa
    28741 acgtggtagc caccgatggg cagtcgagag atggatcgaa aaaaataaaa actgaaagaa
    28801 agcgagtaga tcagctttct ctcagttttt ttatttatta cagtacatat tttttgattg
    28861 cttcagctac accgtgctcg tcgtttgatg aagtgatgac atttgctgct tctttgacaa
    28921 gagaaacggc atttcccatt gctactccta gaccagcata ttcaatcatg gataagtcgt
    28981 tctcgttatc tccgatagcc atgatttcat cttgatcaat accaaggtgc tgtgcgagat
    29041 tcgcaactgc cgcaccttta cttgcttctt tatttagtac ttcgaagtaa tagggcgcac
    29101 ttttgaccgt tgtgtattta tcacggaaag tttgcggtaa acgcgcaatt gctgcatcta
    29161 aaatttctgg ttcgtcgatc atcatcattt tgacgatgtt catgtctggt gtcatttctt
    29221 caggtgttcg atattttagc ggcatcttga ctaaacttgc ttcgtggatc gtataagcgc
    29281 tgatatctcg attaggcgtg tagatcgctt tgtcatcgat cgaatggaga tgactgccga
    29341 cacggcgtgc cattacttcg atctctagga aatcttcatg tgtcagtccg tgacgggcga
    29401 tgattttacc tgatttcgtt tgttgtacga gtgccccgtt ataagtaata acgtaatcat
    29461 tatcttgaaa aaggttcaac tcgtttaact gttcctggac acctggtaag ggacggccag
    29521 tgcataaaac gatcgaaaca ccttttgcaa cagcagcggc aatcgcttct tttacttctt
    29581 ttgttacttc tcttttctca tttaataaag taccatcgat gtcgatagcg actagtttga
    29641 tagacataat ttcctccaat atattttaat ttatttcaat caatgcgcca ttacggatat
    29701 ggctggcaaa ttcttgataa gtttcgtcaa ataaatcata atgatcacgc aaggacggat
    29761 caaccatttc ttttggaaag aaaaatcgct catctccttg ttcttgtccc gctaatgcag
    29821 caacaagctg actaacctgt gataattcta tcaaagttcc gtcatttcgc aaaatctcga
    29881 tttgtgtgcg atgtctgcct tgttctgggc gataaaaatc ataaggtaga tcgtagctag
    29941 aattcacagc agtgtaataa acaggattat atccgacctt ttctactaac aaagtgagtt
    30001 cttggactaa ttttgaatcc cggttgccag aaaaagtagc agatttcagt ggtttacgat
    30061 tcaaaaaacg tttagctaaa tcatttaaaa taggatcagc agattcagac cattgagtaa
    30121 agtaagtgcc caagacccca tcatcaagtt tgaggtattc ttctaaagta aagcttccgt
    30181 caagaaatgg cagaagtaat tgagaatgga gttcaaaact gctggcatct tcttgataca
    30241 actcgtgtgc gcgatgaagc agatgctcca aaattacttc cataccacgg gaaacaggat
    30301 ggaagtacac ttgcacatac atttgatatc ggctaacgat ataatcttcc actgcatgca
    30361 tgccactcat ggaaaaagcg atcccgcctt cataaggacg aatcacacgt aatattcggg
    30421 taagatcaaa tgttccatat tctgttccag tgaaataagc atcccgaagc aaatagtcca
    30481 ttcgatctgc atcgatctga ctagaaatca tttggacaac ttgtgggttg gggtaagttt
    30541 tttggatcac actagctacc ttttcaggaa aaccttcttc tactcggtta agaatctgat
    30601 acacttctgt ttctggagaa gtgatgatct ctacagtgat ggcttcatga ttcgtatgga
    30661 agatatgttc aaatgtatga gagtaaggac catgaccgac atcatgcaac agtgccgcac
    30721 ataaagtcac cagccgttcc tgatcatccc agcctccgtt gcctattttt tcttttgaaa
    30781 aatttcgaga aaagatatcg cagattcgtc ttgaaatctc ataaacgcct aatgaatgag
    30841 taaaacggct atgttctgct ccgtgaaaag tgaaagaaga agtgcctaat tgtttaatgc
    30901 gtcgtagacg ttggacttct ttcgaattga tcaaatctaa gattacttga tgctggacat
    30961 ggatatagtt atggactgga tcacgaaaaa ctttttccat aggcagcatt tgatatttat
    31021 aaggggttgt cataatgtag ctcctttatg ttattacgtt gttccattct ctggatttgg
    31081 ttgttccatt gctccagctg gatttctttt tttagccagt cggtagaatc ctgtaaatca
    31141 cttgcagaat acagcttttc aaaagctttt gcaaatgctt ctttgacttc ttttacaaaa
    31201 aggggttggt caagcagttc ttcaagtgtt gccattgtag caggatccac tgcaggatag
    31261 ccatttgtac caaagtcttc ttttaaagca gattgataaa atcgcttcat gacttgccca
    31321 cgtttttcct gattaccgtt gacacttaag tacatcataa cagaaatccc atttttgatc
    31381 cgacgctgag cgataccggc aaattttttg ccatggatgc ttaaatcata agttccagga
    31441 caataagaat gggtgatttc gaatgcttgt atcggtgcct ttggaaaagc gtatttagtc
    31501 aataggagca tttgctcgta agcgtcatct atggttattt tttttgtgtc actttggggg
    31561 aaaatcaaag aaatattcaa aattccttca tctgatacga tccctaatcc acctgaattt
    31621 ctgatcactg gagcgtaacc tgcatcttcc agtgtttgga tcccgtcact tagaaaagga
    31681 gttcgaacat ccttcatacc taagatgact gtgcagtcat attgccagaa atgtaaaaag
    31741 atttgctggt gttctttaga atattccgtt aatatatctg ttaatgcaaa aggggaaaag
    31801 tcgttttttt gataaatgtg ctgatctagt cgaatcgcag ggttcattca accggctcct
    31861 ttgtataaat tcatattttc tacaatatta tactctaaaa tcaaagagtt gttttggaaa
    31921 aaaggttttc tgtaaaaatg gacaagttgg ttgacaaaac aaaagaaact aggaacaatg
    31981 tagctatgag aagtaaagga gaatgaataa tggaattatc acaaggaaca ccgatttcaa
    32041 tcaaattgcg tacaaaagta aaacagcaag gggaagtgca agatttttat tttgatttga
    32101 aagggcaaat ggtgacaatt ggcgatacgc tgtacatacg ttataaagag attcaggagg
    32161 agacgggcga tgagattcct gtaacgatca aattagttcc agatggtcat gtacagctga
    32221 tccgtgcagg agaaatgcgg atgcgtttga aatttggcta taaagaacgg ttagaaacat
    32281 cctatcgaac accatacggt atgatccaga ttgctacttt tacgaaagaa ctgcatgtta
    32341 gtttgaaaga ccgtcccaca gcagggaagg ttcgaatcga ttatgatctg ttcatgggac
    32401 cggaaaaaat cggcgaatat tatttgacct tggattttac agcgtaaata taaaaaaaaa
    32461 gtttgatttt ttgtgtctga ttttgtatga taaggagtag actgtgaaag gacgtgtcct
    32521 gattggaaat tagtgtattt gaaggtgcaa acaaaagcga attatcaatg atcgaagtag
    32581 cacacgcgat cttagaacaa cgtggcgatg tcatggattt ttctgattta gctaaccaga
    32641 tccaaaacta tcttgggaaa tcagacagtg aaattcgtga ttcactcgca caattttata
    32701 ctgacttgaa tattgacggc agctttatct cattagggga taatcgttgg ggcttgcgtt
    32761 catggtatgc tatcgattcg atcgatgaag aagtaaacca tggaatggac gaagaggatg
    32821 aagatactcc acgtcgccgt aaacgcaaga aagtcaacgc ctttatcaat gatgatgaag
    32881 acgcgatcga ttacaatgac gatgacccag aagatacaga tctgacagaa gaagatgacg
    32941 atgatctgtt tgatgacgat gatgatgaag atgaagaaat cgctgcttat aattctgact
    33001 tgcaagaaat cggagcagat gacccgacag atgatgaaga agacttgcca ggaaatatcg
    33061 aagaagattt aagtatcata gacgaagacg atgatgatga agattatgaa gaggaagaat
    33121 tttctgactc tgaaaaagaa taaacattat tttcttacga gaggttgtga cagagacgat
    33181 cagcctatca gaataaggag gtgtgacacg atttttgtca caccttcttt tttgtatata
    33241 aaaatcccaa gtaaatacat aaaaaataaa aaaattgaaa cttttagcgc accttgtacg
    33301 tcatattaag tgaggcagaa aggagtggag aatgattttt actggatata agaaaaaacg
    33361 gcagatcgaa aaataccgtc acctgctgaa tggactcatc gaaactgatt atcaaaaaat
    33421 gttccgtatc acgatgaatt acgtacatga taaagaagag gccttagatg tgatgcaaga
    33481 tagttttcat aaagccctga actattttga aaaagaaaaa gagatcgagc attttagtgc
    33541 atggttttat cggatattga tccgcacggc attagatagt tggcgcagac aaaaaaggac
    33601 gcctgtggac ttatttgaaa ttcaagatat cccccaacag ttacctttag aaacttccgt
    33661 tgcagagctc catatgattt tagataaaat agacagtccg gaaaaagaga ttctgatcct
    33721 ctactttttt gaaggatttc gtttgaaaga aattgcgact atcttggata tgaatgaaaa
    33781 cacagttaaa acgaaaatgt accgctcatt gaatgcttta cgtcaaatcc tagaatgaag
    33841 gagagaagag tgccatgtat tcccaaaaag aaaatttaaa aaaactaaag aaaacttata
    33901 aagaacaagc aattcccaaa gggcttaaag aggagatccg ccagcgtttt attatcgaag
    33961 agcaatcatt tataaaagca aagcggagaa agagacgtct ccaagggttc ggagtatctt
    34021 tggctatcgt aatgattaca acaagtagcc ttctttttaa tagtcaagtt cgaagctttg
    34081 cagaagattt acccatctta ggatcagtca ttgaattgat cttaggcgaa cgctttactg
    34141 atcgatctga aaaaatcgat attcaggtac cgaagatcaa tacacaaaat gagaaagaaa
    34201 ataaaacaat tcatggatta aatcaaaaat actttcgcga aggacaagca gattttgaaa
    34261 aagctgaaaa agaatatgga aattttgaaa cggatcatta ccaagttttg ggagattatc
    34321 agaaaatcgt agacgataac cggtttctcg tgattgaaag gcagatcacc caaacagcag
    34381 cagatagcca tgttgaaaaa agatacgaca caatcgacaa gaaaaacagt gtccagctct
    34441 cacttccact tttattcaaa gatgacacct atctatctgt tttgacaaaa gaagtcaaac
    34501 ggcagatggc cgaacaagtc aaggaagatc catctaagta ctattggact gagcaagata
    34561 ttcaagaagg gacgatagaa aaaccaacac ttgttacgcc tacacgaagt ttttatttga
    34621 acaaagagca tcaattagtt ttgacttttt ctcaatacga aattgctcct ggttatatgg
    34681 gtacaccaga atttgtcatt cctaagtccg tgacaaaaac gattttggca tcggaagatt
    34741 atttggatca ttagacgaga agtagttgcg actagcagtt tcaattttca agtaagtaca
    34801 agtaaataat aagaagagaa aaagagaaag aagagatata gtgaaaagaa attatttagt
    34861 tatcagtatt attgctttat ccgtgctttt gtttttcgaa attacttcgg tggcctcctt
    34921 gatcaccgaa gtaaaaggac aaacacactc ttcggagcat tcgtcacaat ctttcaaaca
    34981 atcagaacaa tcaaccatta aaagccaatc aactgaaaca gctaacgtca aagaagccat
    35041 tcaagaatca gcggaaaagg tacaaagtca gtcatcacaa tcaacacaag acagtacaaa
    35101 aacgagccga gatccggctg tcaaaagcgt cgcaatcagc tttgatgatg gaccaggagc
    35161 gaccacaaca ccgcagcttc tgcgaatctt aaaagaaaaa aacgtgcatg ccactttttt
    35221 cgtcttagga gaaaatacgg cgcagcatcc tgaaatcgtg aagcagacag ctgaagcagg
    35281 gcacgagatt ggcaatcaca catacgatca tcagaatctg gccactcttt ctgcccagtc
    35341 gatgacggag gaagtgacga aagcagacac tgagatcaaa aaagtcactg gcaaaacccc
    35401 cacttttgtt cgtccgccct atggttctgt cacgaatgta ggagcaacga tcatccagcg
    35461 accgatcatc gaatggtctg ttgattcaga agattggaag acaagaaatc ctgatttgat
    35521 tttgcagaag atccaagcaa cagtatacga tggggcaatt attttattcc atgatatcta
    35581 tcctgaaacg attcgtgccg ttcctcaagt gatcgattat ctgaaagagc aagggtatcg
    35641 catcacgaca gttggggatt tattagggca tccaacggct gtcgagaact attatggcag
    35701 aaacgatcat cggccagttc agtaagtgag acaagtatct ttaataaata acacatttta
    35761 ataagtagtt gaacacataa ctaccttact tgcaaaggct taacaatatc tatacaggta
    35821 caacaaaaaa ctgtccacca attttctttg gcggacagtt ttcacttaaa aataaagaag
    35881 caactattcg taaattatgc taaaatagca gtagaacgcg tcctgaggga atcgaacccc
    35941 cgtctcaaga accggaatct tacgtgatat ccactacact aaggacgcaa gcactgggat
    36001 aattgtaact aaacagataa aaaattacaa ggggattgga caaaaaaatg aaatttgagc
    36061 aaaatttttc acaaaaacag cagcagacac agaaactagc catgacgcaa cagttgcaac
    36121 agtccatcca agttttacaa tataacagtg aggagctgtt ggcattcgtt gagaatcaag
    36181 caatggaaaa tccattggtg gaagttgttg agccggaatg gcagccagat tacataaaag
    36241 cttcttcatc ttcttatgaa ggagaagaga caaattacct aaatcagatt cctgatacaa
    36301 aagggtcttt atttgattca ttgatcgaac aagtccattt gaattaccga gatacttttt
    36361 tgaggaaaat cgttctttat cttgtagaat acatcgattt gaacggattt ttgacgatta
    36421 gtttagaaga agccggaaaa gagattggag ctacgcctat ccaaatgctc gatgcgttga
    36481 ctctgattca gcaattagaa ccagctggag taggtgcacg tagtttgcaa gaatgtctca
    36541 tgcttcaaac agaacgagat gattatgcgc cggaattagc ttatatcgtg ttggaagaat
    36601 gttttgagga actggtggag cgaaaatgga aagaaatcgc acaacgtttt tctgttgatc
    36661 tacatgctgt tcagcagata ttcgactatt tacaaacgtt gagtccaagc cctggccgga
    36721 ttttcgaccg tagcagcgaa ttgtacatta ttcctgatgt ccgggtatta gtggacgacg
    36781 ataaaaatgt acaagttatc tcaaaccgaa aaaatcagcc aaatatacgc tttcaagaga
    36841 gttacttcaa acaaatgtca caacaagcag ataaagaaac tgaaaattat ttaaaagaac
    36901 ggaaacagga attcgaatgg ttgaagaaaa cgatcctaca acgtggggat acaattttgc
    36961 gggttgctca agtcattgtt tcccggcaaa aagctttctt tatcgacaag gaacgtccaa
    37021 tcaagcctct tacgttaaag gaagttgcaa ctgagataga tgtgcatgaa tccacggtca
    37081 gtcgtgcagt caacgggaaa tatttggaaa cagacttcgg tgtatttgaa ttaaaaaaat
    37141 ttttcactac acgaattcct acgaatagta cagaacagac agaagacctt tcagcagata
    37201 cagcgaagaa aaagctgcag gaattagtcg atcaagaaga caaaaacaag ccgttgtctg
    37261 accagaaatt ggttgaattg ctgaaaaaag atgagatagc gatctcaaga cgaacagtag
    37321 caaaatatcg tgatttatta ggaatcccga gttcatcaaa aagaaaacgt tatgataact
    37381 aatcagtcaa aagactagaa aaagtatttc actttttcta gtctttttag ttgaaaaacg
    37441 agagcaaaac tgttagactt acagtcgtga ggttgagatt tccctttatt gatgtgaaat
    37501 tcgaatagtt aatttctttt tttatcgtat atgggtcgtt ttaagtctac attggacgta
    37561 aagtgtccaa ctaaggagat cgctatgctg gatgaaataa aaatgattga atctgttgca
    37621 ccagatatta tcgaagtgtt gcaagaacgc tataaaattt tacgaaatat ctattggatg
    37681 cagccgattg gacgtcgaag tttatcggaa agtatgggga tcacagaacg agtattgcga
    37741 acagaaacag atcttttgaa aaatctgaat ttgatcgaca cctcaaaaag cggtatgacg
    37801 ctgacaacta agggcgaaga agtttatcaa agccttgaaa acttcatgga tcagctttta
    37861 ggtacgcatc aaaccgaaca acagttggca gaatattttg gtaaccaacg ctgtatcgtt
    37921 gtttcaggaa acagcgaaga gcagaccaaa gtggctgatg cattcggcga agcgctgagc
    37981 gaagccctag accgactact tccagaaggc gagaatatca tcgctgtgat gggaggaact
    38041 actatggctg tggtagccga acaattgtct aatctggaaa ataagaaacg gcataacctg
    38101 tttgtccctg caagaggcgg gattggtgaa gcgataactg tacaggcaaa ttctgtcagt
    38161 gcaagaatgg ctgctaaagc aaacggtaac caccgtgcac tttatgttcc ggaacaatta
    38221 agtctggcaa cttacaattc tttactcaat gagccatcta tccaagaagt cttaaacttg
    38281 attagtgaag cgaattgtgt tattcatagt attgggcgtg cattacacat ggcagcacgt
    38341 cgtaagatga ctgaaaaaga attagtcatg ctgaaacaag caaatgctgt tgcggaatct
    38401 tttggctact ttttcaatga aaaaggtgaa gtcgtttata aggtccctag aatcggcata
    38461 gaattgcaag atttggaaaa agtcccgatc atcatggcaa tcgcgggcgg aaaatcaaaa
    38521 gccaaagcaa ttcgagctta tatgaaaaat gcaccaaaac aaacgtggct catcactgat
    38581 gaagctgctg caaatgagat tttaaaaggg gtaacccttt aaaataaata tttttttgat
    38641 tttcataagg aggaaatctt aaatgacagt taaagtaggt attaatggtt ttggacgtat
    38701 tggacgtcta gcattccgtc gtatccaaga tgtagatgga atcgaagtag tagcaatcaa
    38761 cgacttgaca gatgctaaaa tgttggcaca cttgttaaaa tacgacacaa ctcaaggacg
    38821 tttcaacgga acagttgaag ttcatgaagg ttcattcaac gtaaacggaa aagaagttaa
    38881 agtattagct aaccgcaacc cagaagaatt accatggggc gaactaggcg tagatatcgt
    38941 tctagaatgt actggtttct tcacttctaa atcagcagct gaaaaacatt taacagctgg
    39001 tgctaaacgc gttgttatct ctgctcctgg cggaaacgat gttccaacaa tcgtttataa
    39061 cacaaaccac gaaacattaa ctggtaaaga aacagttatc tcaggtgctt catgtactac
    39121 taactgttta gctcctatgg ctaaaacatt gaacgacaaa tttggtgttg ttgaaggatt
    39181 aatgacaact atccacgctt acacaggtga ccaaatgact ctagacggac ctcatcctaa
    39241 aggtgacttc cgccgtgcac gcgctgctgc tgcaaacatc gttcctaact caacaggtgc
    39301 tgctaaagct atcggtttag taattcctga attgaacggt aaattagacg gagctgctca
    39361 acgtgttcct gtaccaacag gttcattaac agaattagta acagttcttg aaaaagaagt
    39421 aactgttgac gaaatcaatg cagcaatgaa agaagcttca aacgaatctt acggatacaa
    39481 cacagacgaa atcgtttctt cagatatcgt tggtatgact tacggttcat tatttgatgc
    39541 tacacaaact aaagtaatga cagttggcga caaacaatta gttaaaactg ttgcttggta
    39601 cgacaacgaa atgtcataca ctgcacaatt agtacgtact ttagaatact tcgctaactt
    39661 ataagataga caaacgtaca agtgtaaatt atagacatgt acatttaaaa gcggggaagc
    39721 aatcgcgctt cttccgcttt ttttgataat aagtttattc aggaggaaat aaaatggcta
    39781 agaaaacagt gaaagatatc gatttaaaag acaaaaaagt tcttgtccgt gttgacttca
    39841 acgttccttt gaaagacggc gtgatcactg acgacactcg tatcaaagca gcattgccaa
    39901 caatcaacta cgttctagaa caaggcggaa aagcaatcct tttctctcac cttggacgtg
    39961 taaaaacaga agaagataaa gaaggaaaat ctttagctcc agttgcaaaa cgtctaggcg
    40021 aattattagg taaagaagta acattcgtac ctgaaactcg cggagaacaa ttagaagaag
    40081 caatccgcaa catgaatgac ggcgacgttg tcgtattcga aaatacacga tttgaagata
    40141 ttgacggtaa aaaagaaagc aaaaatgata cagaactagg taaatactgg gcttcattag
    40201 gcgatgtatt tgttaacgat gctttcggta cagctcaccg tgcacatgct tctaacgtag
    40261 gtatcgcttc aactggtatc ccaacagttg ctggattctt gatggaaaaa gaaatcaagt
    40321 tcatcggtga agcagtagaa gaaccaaaac gaccaatgat tgcaatcctt ggtggcgcaa
    40381 aagtttctga taaaatcggc gtaatcgaaa acttgattcc taaagcagat aaaatcttga
    40441 tcggtggcgg aatgacttac acattctacg aagcaaaagg aatcaagatc ggtaactctc
    40501 tagtagaagc tgataaagtg gaactagcaa aagaattaat cgaaaaagca ggcgacaaac
    40561 ttgtcttgcc aatcgacaat gtctgtgcac cagaattttc aaatgatgtt gaaacacaag
    40621 taatcgaagg agacattcct gatggtttga tggctttaga catcggccca gcatcagtaa
    40681 aattatttgc tgatacatta caaggtgcaa aaacagttgt ttggaacgga cctatgggtg
    40741 tatttgaaat gagtaacttt gctaaaggta caatcggtgt ttgtgaagca atcgctaact
    40801 tagaaggcgc aacaacaatc atcggtggcg gagactcagc cgcagcagca gaacaattag
    40861 gatttgctga caaattcaca cacatctcaa caggtggcgg cgcaagcttg gaattgcttg
    40921 aaggtaaaga attaccagga ctagcagcaa tcaacgataa ataaacgacc aagtttgctc
    40981 aattctgacg agtaatcgat caaaaggtga agggagtttt catgactccc tagccatttt
    41041 tcaataaatt aaggagtgtg ttcctcaatg cgtaaaccta ttatcgcagg taactggaaa
    41101 atgaacaaaa ctgcatcaga agcaaaagaa tttgctgaag cagtaaaaaa caaaatccct
    41161 tcaaatgaca aagttgattc agtcatcgga tctcctgcat tattcttaca agaattaatg
    41221 acatcagctg aaggtacaga actaaaaatc tcagcacaaa actgctactg ggaaaactca
    41281 ggtgcgttca ctggtgaaac ttcaccagca gctcttgctg atctaggtgt tcattatgtg
    41341 atcatcggac attcagaacg tcgtgaatat ttccatgaaa cagacgaaga catcaataaa
    41401 aaagcgaaag ctatctttgc aaatggtatg acaccaatcc tatgctgtgg cgaatcttta
    41461 gaaacctatg aagcaggcaa aactgctgaa tggatcgaag gacaaattac agctggttta
    41521 gctgatcttt cagctgaaca agtgtcaaac atggttattg cttatgaacc aatctgggca
    41581 atcggtactg gtaaatcagc tgacgcacaa atcgctgacg aaatttgtgg agttgtacgt
    41641 caaacagttg aaaaacttta tggaaaagaa gtatcagaag cagttcgtat ccaatacggc
    41701 ggttctgtaa aaccagaaaa tattgctgaa tacatggcaa aagaaaatgt tgacggtgct
    41761 ttagtcggtg gcgcaagctt gcaagcagat tcattcttgt cattgttaga cgctgtaaaa
    41821 taattgttga aaatttgcga aaaatgcaag aaatgtttgc actaatgagc aaaattaact
    41881 aaaatcgtat atgagggaaa tcccttagga aaaaacaaac tcaaaggaga gacaaaacat
    41941 gtcaattatt actgatgttt acgctcgcga gatcttagac tcacgtggta acccaacaat
    42001 cgaagtagaa gtatacacag aaagcggagc ttttggccgc ggaatggttc cttcaggtgc
    42061 ttcaactggt gaatacgaag cagttgaatt acgtgacgga gacaaagctc gttatggcgg
    42121 taaaggtgta actaaagctg tcgacaacgt aaacaacatc attgctgaag caatcattgg
    42181 ctacgatgtt cgtgaccaaa tggctatcga caaagctatg atcgctttag acggaactcc
    42241 taacaaaggt aaattaggtg cgaacgctat tttaggtgtt tctatcgctg ttgcacgtgc
    42301 agctgctgac tacctagaag tacctttata ccactaccta ggcggattca acacaaaagt
    42361 attgccaaca ccaatgatga acatcatcaa cggcggatca catgctgata actcaatcga
    42421 cttccaagaa ttcatgatca tgcctgttgg cgctccaaca ttcaaagaag ctctacgtat
    42481 gggtgctgaa gtattccacg cattagcttc aatcttgaaa gctcgcggat tagcaacttc
    42541 tgttggtgac gaaggtggat ttgctccaaa ccttggttca aacgaagaag gttttgaagt
    42601 aatcatcgaa gcaatcgaaa aagctggcta tgtacctggt aaagacgttg ttcttgctat
    42661 ggatgctgct tcttcagaat tctacgacaa agaaaaaggt gtttacgtac tagctgattc
    42721 aggcgaaggc gaaaaaacaa ctgacgaaat gatcaaattc tacgaagaat tagtttctaa
    42781 atatccaatc atctcaatcg aagacggatt agacgaaaac gactgggacg gattcaagaa
    42841 attaactgac gtattaggcg acaaagttca attagttggt gacgacttgt tcgtaacaaa
    42901 cactcaaaaa ttatctgaag gtatcgaaaa aggaattgct aactcaatcc ttatcaaagt
    42961 gaaccaaatc ggtacattaa cagaaacttt cgaagctatc gaaatggcta aagaagctgg
    43021 ctacacagca gttgtatctc accgttctgg tgaaacagaa gattcaacaa tctctgatat
    43081 cgctgttgca acaaacgctg gccaaatcaa aactggttct ctatcacgta ctgaccgtat
    43141 tgctaaatac aaccaattac tacgtatcga agaccaactt ggtgaagttg cagaatacaa
    43201 aggtttgaaa tctttctaca acttaaaaaa caaataattc ttttctaatg aagatacatg
    43261 atcgactatt gaaagatagt cgagtatgat cacttagtta agtaatcgaa ctctctactt
    43321 gctgaaatat cagcaggtag agagtttttt ttgcaactag cttctgtata tgatgtttac
    43381 aaagaatggt acaagaaaag atcatccttg aaaaaggaaa gctaacaata actagcatca
    43441 tataagagct tttaaggata gaacagtaga ataggaatct atgagcaagt cctctcgtat
    43501 aaataaaata ggagggaagt agcagcatat ttttcataaa gtggctctct ttcttcattt
    43561 ttactttggt tgctcctatt tgaaaattcg agcacactca aatacaataa aaagaaatgg
    43621 caagggagag gattagatga aaaaagaaga aaaaatcgcg attttacaag agatcatccg
    43681 aattaaatca gtgaatggaa acgaaggtga agtagcagcc tatttgaata agcttttggc
    43741 aaagcatgac ataacaggag agattgtttc ttacagagat ggtagagata atttgattgc
    43801 tcgttaccaa aagggccaat caggaaaagt gttaggccta tctgggcata tggatgtcgt
    43861 ggcggctggt gatgaatcgt cttggaccta tgcacccttt gcagcagaaa tccatgggaa
    43921 ccgtttgtac ggacgtggtg ctacagatat gaagagtggc ttggcagcaa tggtgattgc
    43981 catgatcgaa ctgaaagagt caggaaaacc atttaacgga acagtcaaat tattagccac
    44041 tgtcggagaa gaagtgggtg aactaggtgg ggaacagctc acgaaagcag gctatgtaga
    44101 tgacttagat gccttgatca tcggagagcc aactaattac agtttgatgt atacgcacat
    44161 gggatcaatc aactatacag tcacctcgca cggaaaagaa gcgcatagct ctatgccaga
    44221 ccaaggttat aatgcaatca atcacctaaa tgaattcatt acgaaagcaa atgctgaaat
    44281 gaatcacctc gctgaaacaa tcgagaatcc tgtcctagga aaaacaatcc ataatgttac
    44341 attgatcagt ggcgggaatc aagtcaacag catcccaagc catgcgcagc tccaagggaa
    44401 cattcgatcg attccagaat acccaaatga taaaatcatt gcattgctgc aatcaatcgt
    44461 caatgaattg aatcaagaga cagactatca tttggaattg atgatcgatt acaacaaaat
    44521 acctgtcaaa gctgatccag attctccttt gattcattgc attcagcaac aattcagcca
    44581 gccattacct ttagttggag cggcagcaac aacggatgca gccgaattta cgaaagctaa
    44641 ccattccttc gactttgtcg tctttgggcc tggtgtcgtt actttgcctc atcaagtaga
    44701 tgagtatgtt gaaatcgata attatttgga tatgattgaa aaatatcaag gaattatttt
    44761 atcctactta gcgtaagatt tttttaataa atgaaaaaac agagccagat tagcattatt
    44821 ttgctaatcc agctctgttt attcagccta tttttctttt tgaatatatc aagcgttgtg
    44881 gcaacatagt gatgactaat gaaacaacga cgatagacaa caaacgatca aggtagtctg
    44941 tacctgcctg taccaagatc acacttgttg tctgacttaa acctaaccca tgtaatagct
    45001 gaacgatcat actggatcct gaggaggtga tcccgccaaa taactgaacg gtaatgatcg
    45061 aagaaacgat cgttccaggt accgacaaaa tcaaagcgag taataacagt ttcaagctgt
    45121 ttttatcttt gccgagatat cttggcaaaa tcaatccagc aagcaagcct gtgagaattt
    45181 ggattggcga ataatacaga gaaaaaatat ctgtagtcac tccacttaaa ataccgctgg
    45241 acaatcctgt cagcattcca ggtacaggcc caagcaatgc accagcaaaa atcgttccga
    45301 tagaatcgag atagattggc aaacgaagcc ataaagcaat cgttcctcca atataattga
    45361 tcccaataca caaagccatc actgtaagta cgcgagttgc cattttgttt ttattcattt
    45421 tgttagccca tctttaacct ttccaaatct ttcataatcg tttcttcttg tgcatttaaa
    45481 agaacggtta aaaactttcg gaaaaaaaga cgagtatcta cttttgtcat aattttactg
    45541 ttagcaggtt tctgccaaaa ttcataacga tcaaccaatg tttgtccacg acttattcct
    45601 tgagtttcca cagcagtgaa gcttttgaaa ccttcacaga tggccccctc gataaaataa
    45661 gcaacagcta acggatcatt gatgacacat cctaaaattc tttcttgttt ccagtggaag
    45721 tcaaaataaa aacgagtgat cgcttttaga tactctccct cttcaggatt catttgacag
    45781 cagtattcga ggatagtcgg tgtcaagacg atttctcttg tcacatctaa cccaaccatt
    45841 tctatcgttt gtttgagatt ctcgaataca taagaagctg cttcaggatc acaccagtaa
    45901 ttgtattcag caactggcga acagttacca tggcttttgt aagttccacc catcgagaca
    45961 aaacgatcca tatgtttccc aatatttggg ttgactttca aagcaaaagc gatattggtc
    46021 aaaggaccca aagcaatgac tgatgtattt gtcttttctt tgaaataatc agccaaaaaa
    46081 tcaacggcat ggagaggttc tgcctgtttt ctcaagatca tcgggaaatt tgtttcccct
    46141 aaaccatcca taccatgagt gtcttgtgca ctgatgaaag gttgcttcaa aggtttttcc
    46201 attccttgat aaacaggaat gtctaaacga tcacagcgtt ccaggcattt cagcacattc
    46261 tgcgtaccta tatctgcagg gacattccca caaactacgg taatagcaac tacttcgata
    46321 tcaggggatt tgacagctaa tgataaagct aaagtgtcgt cgatccctgg atcgcaatca
    46381 ataattactt ttcgcattat ttccaatctc ctttctttag agatacttag ttttttattc
    46441 tgggaagttt ccgaaccagt ccaagacgag cgtcttgatt ataatgcacg aaattagtat
    46501 actggctttt attgcgaatg taaatgctct cttacaagta aatctattga ggagaacttt
    46561 aagtaaatga tacaatgaaa aagaaatgga atttacgtgt gccgaaaata aagaatccta
    46621 tggatcggta cattggataa tagttagatt atagtttggc agaaagcggc gggtgtacaa
    46681 tggttatttt ttatttcatt cttattctga taattatagg gatcagttac cattttcttt
    46741 cttattttta tgcaaagaaa ttagtaagag cgggagtaca gagaggatat gactggtatg
    46801 aagacactgg tcatcgtttg atggaccctg ctgcagtcac gtcagtagaa aaaaaacgac
    46861 aaaaattaga agaagagtgt gaaagctttt gggagcaggg aacacccaaa acaatcaaaa
    46921 gttatgacgg gttgaaacta gctggtcaga tgttcatcca tccaaataag cagaataaat
    46981 gggtcatttg cgtacatgat taccgaagca cagggaaaag agacatgtct catataggta
    47041 aaagatatgc agagaaagga ttcaacgttc ttgtacctga tttacgtgcg cacggagaaa
    47101 gtgaaggaga aatcatcgga atgggatggc tagatcggct agatttaatt gcctggatcc
    47161 aactcatctt agatgaacag ccgaatgcgt ccattatttt acacggcgga tcaatgggag
    47221 cttctacgat catgatggca agtggtgaaa aacttccatc agcggtcaaa ggatttattt
    47281 tagacagcgg atatgtttct gtttatgcag aattccgata tatgttaagt aaaatcactg
    47341 tctttcctaa aaaaatggtc atgagatatg cgaatcatta tgcccagaaa tacgccggtt
    47401 actccttaaa gcaagcatca gctacgagac agcttggcag caaccatctg cctttattgg
    47461 tcattcatgg tgaaagagat cattttgtcc ctacagaagc agcgtacacg attcaaaatg
    47521 ctacggcagg ggataaagca ctgctgcttg ttccagaagc agaacacttg gaagcatcga
    47581 tgaaagatcc gaaaacctat tggacagtga ttttttcatt tattgaacaa agaataacga
    47641 tgaattaatt ttctcttctt ttttgtaagt aattcgagga taaataattg tttatagatc
    47701 aggagactgc taaaataagg taggaataaa aaaatatcag ttatttgagg agtgcggcat
    47761 atggaaatca aagaagaaaa aaatcgtctt gctttattca atgatgaaca acaagagatc
    47821 ggagaaatga catggtcaga tgcgggccca gacatcatga tcattgacca tacatttgta
    47881 gatccagcat atcgtggtca aaagttagct gaaaagctag tgtatactgg agtagaatta
    47941 gcaagacgag acgggaaaaa gatcattcct ttatgccctt atgcaaaaaa agagtttgaa
    48001 aagaaaccag aatatcaaga cgtcttacgt cagtcataag aataacagct gaaagttttg
    48061 aactttcagc tgttattcca tttatataga gttgtcacgg acttttatct atgataaagt
    48121 taaagagacg atatcttttg ggaaggaggc tgcgatatgt ataatttaat tttgggaatc
    48181 gtgatcgttt tatcgattgt tatggtcatt gcgattatga tgcagccaag caagcaaaat
    48241 agtgcagcga gtgcatttac cggcggtgcg gatcaactat tcggcaaaca aaaagctcgc
    48301 ggctttgaag ctgtcatgca gcgatcaact gcagttatgg gagctatctg gatgatctta
    48361 ttatttgtcc tagcatattt atcttcgaag tagttgaata agcctctcgc aaaacggcgg
    48421 gcggcttttt ttattgcaaa gagaacgctt aagaagaagt tatgaaagaa actatggtaa
    48481 aatgaggcga aagaggtgag acgagtgaga caattaccaa aaccccttta tgcaaaacat
    48541 ggaaagcgag cggttttgct gcttcacgct tactcaggaa gtccaaacga tgttcgaatg
    48601 ctcgcacgat ttttggaaaa agcagattat acagtttatg cgccgctttt taaaggacac
    48661 ggaacaatgg agccatatga tattttgcaa gaaaaagcag aaagctggtg ggcagacact
    48721 aaaaaagcca tccatttttt acagtctgag cagttttctg atattgccgt attaggatta
    48781 tcaatgggcg gcatctttgc tgtacgagca ctagaagaag aatcagtgat tggcggcggt
    48841 tttttctgtt cgcctttgtc tcctgtaaaa acaaacgtgc ctgaaaactt tgaaaaatac
    48901 gtacgtcaag tgctgaagac agcaggtaaa tcagagaaag aaatcaatga aaaagcagtt
    48961 gcttaccgct cgttagctga acaacaatta atggatatac aagatcaggc agcaatcgta
    49021 gaaagtaggt tgtcagatat ccaacagccg atatttttag ctcaagctgg gaaagatgaa
    49081 atgatcgacc ctaatggcgt atttgaaact gcacgcaagt tgagccgtca gcgagtgaca
    49141 cttcgatggt atcctgaaag tggtcatgtg attacagttg gtacagcaag acgcgaatta
    49201 gaaaaagacg tcttggaatt tttagaaaaa ttaccttgga atgaggaata aatatgacaa
    49261 aagaaacgat aaaagagaaa attctctttt tcatggaaag cagtcgcaaa cgcagttttt
    49321 ctatggaaga aatcgctgaa ggtttagggt tccaaaaaag cgatgatttt aaattactag
    49381 tacaaacgat tgctcaaatg gaaagagaac agagtatagt tttcaataaa aaaggaaaag
    49441 tgaaacttcc tttaaaacca atcttgatcg aaggaacatt tcgagcaaat gaacgaggat
    49501 ttggatttgt aacgattgat cccgaagaag atgacatcta tatcccaaaa gaagcgactg
    49561 gttttgcaat ggatggagac accgtagcaa tcgatatcat gaagcgagca aatacagata
    49621 tggatcgcgg agcagagggg aaagtagtcg agattcgaga ccgggcgact actcagttag
    49681 caggggaatt cgttgcttat tcagaagaag aaatacaaga aacggatctt tacggcgttg
    49741 ttatcccgaa agatagaaag ctcaaccaat ttaaagtata tgcagcagca gagggagttc
    49801 gtccagttga cggaagtatt gtgctgatcg aactgaccca ttaccctgaa aaagggtatg
    49861 cgactagttt agaagggata atcaagcaag tgattggtca taagaatgac cctggcatgg
    49921 atatcctctc aattgtcgtg gctaatggta tccctacgaa atttcctgat gatgtattgt
    49981 ctgaagcaga tgaagttcca gatcaaatca gcgaaaagga tataactgga cgtagagatt
    50041 taagaaatca gctcattgtt acgattgatg gagaagatgc aaaagatcta gatgatgcag
    50101 ttacggttaa aaaattagat aacggtaatt attttcttgg agtacacatt gcagatgttt
    50161 cttactatgt gacacaaggc agtcaattgg acagagaagc atatgaaaga gggacaagtg
    50221 tatatttgac tgatcgagtg attccgatga tcccgcaacg tttatcgaat ggtatctgtt
    50281 cactgaatcc ccatgttcca aggctgacca tgagttgcga aatggagatt gatccaaatg
    50341 gacaagtagt ctctcatgat attttcccaa gtgtgatcca aacaacagaa cggatgacct
    50401 acacagctgt caatcaaatc ttagaagatc aagacgaaga aacgatgcaa cgctatgctc
    50461 agctcgtacc tatgttccaa gaaatgggtg aattgcatcg aatccttgaa acgatgcgaa
    50521 tcaaacgtgg cgcgatttct tttgaggatc gcgaagcaaa aatccttgtt gatgctgaag
    50581 gacacccaaa tgatatccag ctaagaagca gaggaattgg tgaacgcttg atcgaatctt
    50641 tcatgcttgc ggccaatgaa acggtagctg aacattataa taaattaaat ctgccgttta
    50701 tttaccgaat ccatgaacag ccaaaagaag aaaaaatgca gcgcttcttt gattttgctt
    50761 cagctttggg cattctggtc cgaggaacga aaggaacgat cacaccaaaa gatttacagc
    50821 atgtgatcga aggagtagaa gacaaaccag aagctgcagt tatcaatact atgttgctaa
    50881 gaagcatgca acaagcacgc tattcagaag acaattttgg gcattatggc ttagcggcag
    50941 attactacac acactttaca tctcctattc gacgctatcc cgatctaatc gtccatcgtc
    51001 tgatccgttc ttacagtgaa gataaaagtg aaagcaatca aagttcgtgg gctacggaac
    51061 ttcctgaaat tgcagatcat agttctaaga tggaacgtcg tgcagtagaa acagaacgtg
    51121 aagtagattc catgaagaaa gcagaattca tgatggacaa agtgggggaa gaattcgatg
    51181 ggatcatcag ctctgttgtg aaattcggcc tgtttatcga actgccgaat acagtagagg
    51241 gattgatcca tatcaatgaa ttgaaacagg actacttcca ttttgttgaa aatcatttag
    51301 ctcttgtagg tgaaagaact ggtctgactt ttaaaatcgg acagaaagtg cgagtaaaag
    51361 tagtcaaagc tgatccagat gaacgagcag tagattttga acttgtttcc gcagaagagg
    51421 ttacagcgtt ggaacggcca aaaacacgaa atggaaaatc aggaaaacgc gaaggaaaac
    51481 aagcaggaaa acgcccagaa agaaatggca agaagaacaa acgatatagc gatagtaagc
    51541 aaacgttcgg taagaaaaaa gggaagaagc cattttacaa aggtgttaaa aagaagaaaa
    51601 agaaaaaata aaatgagttg gaggaaacag ctatgccaaa aggcgaggga aaattaattg
    51661 cacaaaacaa gaaagctcgc catgattatt cgatcatcga cacgatggaa gcagggatgg
    51721 tcttgcaagg aaccgagatc aagtcgatac gaaacagccg gatcaatcta aaagatggat
    51781 ttattcgcgt ccgcaacgga gaagctttct tgcataatgt tcatatcagt ccttatgaac
    51841 aaggaaatat ttttaatcat gatccgttgc gcacgagaaa gttattatta cacaaaaaac
    51901 aaatcatccg gcttgaaaat gaattgaaaa atactggaat cactgttgtt cctttaaaag
    51961 tctatattcg taacggctat gccaaggtat tgattggtct ggcgaaaggg aaaaaatctt
    52021 atgataaacg ggaagatttg aaacgaaaag atatcgatcg acaaattgat cgaacattaa
    52081 aaaatttctc tagataattt cattatcctt tttcgc
//
LOCUS       DAHHZJ010000002        50578 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.002, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000002 DAHHZJ010000000
VERSION     DAHHZJ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.002"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..377
                     /locus_tag="NBL02_000051"
     CDS             <1..377
                     /locus_tag="NBL02_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HCD2570769.1"
                     /translation="KDAISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEI
                     VPQNVSKGVVFDRLESIYNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKA
                     DYVTVSNDKDGVSQAFERILTI"
     gene            658..1878
                     /locus_tag="NBL02_000052"
     CDS             658..1878
                     /locus_tag="NBL02_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HCD2570770.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            1875..4595
                     /locus_tag="NBL02_000053"
     CDS             1875..4595
                     /locus_tag="NBL02_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCD2570771.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            4579..5523
                     /locus_tag="NBL02_000054"
     CDS             4579..5523
                     /locus_tag="NBL02_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCD2570772.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(5592..6602)
                     /locus_tag="NBL02_000055"
     CDS             complement(5592..6602)
                     /locus_tag="NBL02_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCD2570773.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(6782..7126)
                     /locus_tag="NBL02_000056"
     CDS             complement(6782..7126)
                     /locus_tag="NBL02_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCD2570774.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7119..7553)
                     /locus_tag="NBL02_000057"
     CDS             complement(7119..7553)
                     /locus_tag="NBL02_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HCD2570775.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            7691..8428
                     /locus_tag="NBL02_000058"
     CDS             7691..8428
                     /locus_tag="NBL02_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2570776.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            8421..9632
                     /locus_tag="NBL02_000059"
     CDS             8421..9632
                     /locus_tag="NBL02_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2570777.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            9836..10618
                     /locus_tag="NBL02_000060"
     CDS             9836..10618
                     /locus_tag="NBL02_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HCD2570778.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            10688..11350
                     /gene="trmB"
                     /locus_tag="NBL02_000061"
     CDS             10688..11350
                     /gene="trmB"
                     /locus_tag="NBL02_000061"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCD2570779.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(11448..13202)
                     /gene="ade"
                     /locus_tag="NBL02_000062"
     CDS             complement(11448..13202)
                     /gene="ade"
                     /locus_tag="NBL02_000062"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCD2570780.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            13334..14263
                     /gene="tadA"
                     /locus_tag="NBL02_000063"
     CDS             13334..14263
                     /gene="tadA"
                     /locus_tag="NBL02_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HCD2570781.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14220..15269
                     /locus_tag="NBL02_000064"
     CDS             14220..15269
                     /locus_tag="NBL02_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HCD2570782.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            15266..15544
                     /locus_tag="NBL02_000065"
     CDS             15266..15544
                     /locus_tag="NBL02_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCD2570783.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            15519..15989
                     /locus_tag="NBL02_000066"
     CDS             15519..15989
                     /locus_tag="NBL02_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HCD2570784.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16006..16308
                     /locus_tag="NBL02_000067"
     CDS             16006..16308
                     /locus_tag="NBL02_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2570785.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            16325..16696
                     /locus_tag="NBL02_000068"
     CDS             16325..16696
                     /locus_tag="NBL02_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HCD2570786.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            16668..17027
                     /locus_tag="NBL02_000069"
     CDS             16668..17027
                     /locus_tag="NBL02_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2570787.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17199..18206
                     /locus_tag="NBL02_000070"
     CDS             17199..18206
                     /locus_tag="NBL02_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCD2570788.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18231..19415
                     /locus_tag="NBL02_000071"
     CDS             18231..19415
                     /locus_tag="NBL02_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCD2570789.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(19852..21435)
                     /locus_tag="NBL02_000072"
     CDS             complement(19852..21435)
                     /locus_tag="NBL02_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCD2570790.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22013..22156)
                     /locus_tag="NBL02_000073"
     CDS             complement(22013..22156)
                     /locus_tag="NBL02_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCD2570791.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(22378..22857)
                     /locus_tag="NBL02_000074"
     CDS             complement(22378..22857)
                     /locus_tag="NBL02_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCD2570792.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(22930..23214)
                     /locus_tag="NBL02_000075"
     CDS             complement(22930..23214)
                     /locus_tag="NBL02_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2570793.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(23292..23486)
                     /gene="ssrS"
                     /locus_tag="NBL02_000076"
     ncRNA           complement(23292..23486)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="NBL02_000076"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(23558..24844)
                     /locus_tag="NBL02_000077"
     CDS             complement(23558..24844)
                     /locus_tag="NBL02_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HCD2570794.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            24982..25653
                     /locus_tag="NBL02_000078"
     CDS             24982..25653
                     /locus_tag="NBL02_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HCD2570795.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            25619..26107
                     /locus_tag="NBL02_000079"
     CDS             25619..26107
                     /locus_tag="NBL02_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HCD2570796.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26120..27067
                     /gene="prmA"
                     /locus_tag="NBL02_000080"
     CDS             26120..27067
                     /gene="prmA"
                     /locus_tag="NBL02_000080"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCD2570797.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27068..27832
                     /locus_tag="NBL02_000081"
     CDS             27068..27832
                     /locus_tag="NBL02_000081"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCD2570798.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28065..30278
                     /locus_tag="NBL02_000082"
     CDS             28065..30278
                     /locus_tag="NBL02_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HCD2570799.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            30289..30735
                     /locus_tag="NBL02_000083"
     CDS             30289..30735
                     /locus_tag="NBL02_000083"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCD2570800.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            30906..32165
                     /locus_tag="NBL02_000084"
     CDS             30906..32165
                     /locus_tag="NBL02_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HCD2570801.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(32254..33201)
                     /locus_tag="NBL02_000085"
     CDS             complement(32254..33201)
                     /locus_tag="NBL02_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCD2570802.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            33595..34887
                     /locus_tag="NBL02_000086"
     CDS             33595..34887
                     /locus_tag="NBL02_000086"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCD2570803.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            34902..36671
                     /gene="aspS"
                     /locus_tag="NBL02_000087"
     CDS             34902..36671
                     /gene="aspS"
                     /locus_tag="NBL02_000087"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCD2570804.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            36780..37655
                     /locus_tag="NBL02_000088"
     CDS             36780..37655
                     /locus_tag="NBL02_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCD2570805.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            37921..39804
                     /locus_tag="NBL02_000089"
     CDS             37921..39804
                     /locus_tag="NBL02_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCD2570806.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(39879..40163)
                     /locus_tag="NBL02_000090"
     CDS             complement(39879..40163)
                     /locus_tag="NBL02_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCD2570807.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40153..40887)
                     /locus_tag="NBL02_000091"
     CDS             complement(40153..40887)
                     /locus_tag="NBL02_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HCD2570808.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41016..41639
                     /locus_tag="NBL02_000092"
     CDS             41016..41639
                     /locus_tag="NBL02_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HCD2570809.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            41831..42952
                     /locus_tag="NBL02_000093"
     CDS             41831..42952
                     /locus_tag="NBL02_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HCD2570810.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            42959..46087
                     /locus_tag="NBL02_000094"
     CDS             42959..46087
                     /locus_tag="NBL02_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HCD2570811.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46106..49300
                     /locus_tag="NBL02_000095"
     CDS             46106..49300
                     /locus_tag="NBL02_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HCD2570812.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(49446..50153)
                     /locus_tag="NBL02_000096"
     CDS             complement(49446..50153)
                     /locus_tag="NBL02_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCD2570813.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16524 a         8873 c        10525 g        14656 t
ORIGIN      
        1 aaaaggatgc tatcagcatt tacaaatttt ttatttttat tcctaatcgt caattgaaag
       61 ctaatatatt taaccatttg aaaaagatgc taaatgtgtc tgttactgaa tcggcacctg
      121 taaatattga aatcgtacct caaaatgtat ctaaaggtgt ggtgtttgat cgtttggaat
      181 caatctacaa tttaaagaaa cctttgagaa tcgcgatcgg agatagttta aatgacttat
      241 ctatgtttga aagtgcagat attagttttg caatgggaaa tagtcatcaa gaaatcatga
      301 aaaaagctga ttatgtcact gtttcaaatg ataaagacgg agtatctcaa gcatttgaaa
      361 gaattcttac catataaact aattaaggta gtatagaaca tagaattcta aatacggaaa
      421 aatggagcat aaattccagt acaaatagta cttcttcagt gtcaaacgaa gcgaatataa
      481 catagatatt taggattgtg acaaaaatca tgtcacaatc tttttttcga ataaacttat
      541 tttttgaagt cggccattta tcgtaatttc tttgcataat acgaacaaat tttcggttat
      601 tgatgaaaaa aaacattgaa aatggtatga taaaagaaaa aaaggaggaa atttttaatg
      661 gtgaaattta tccatgcggc agatttacat atggatcggt catttgaagg actgacaact
      721 cttgataaaa ctgtacagga aaaactattg aagacgaatc tgaccgtttt atcaaatatc
      781 attgaacagg caataataaa taacgttgat tttgttttac ttgcaggaga taattttcat
      841 caaaatcgtc cttcattaaa aatacagaaa catttttcag aacagatgaa acggttagaa
      901 aagaaccata tacctgtatt tataatattc ggaaatcatg atttctatca aaaagagcgg
      961 tattggttct cctttcctaa aaatgtccat ttatttacat ccgaaacggt agagacaaaa
     1021 aaaataacga taaaaagtgg agaaactgtc tctctcagtg gatttagtta tcgtcaaccg
     1081 tggattcaaa aagacaaagt gatggaattt ccttctagag aactcacaga ttaccatatt
     1141 gggttgtacc acggggaacc tggcgtctct caaaaaggaa actatgctcc tttccaaccc
     1201 tcaaagatgc aagaaaaggg atacgattat tgggctttag ggcatatcca tgtgccaacg
     1261 gtcttaaatg aaaagcaaac aattgtttat ccaggtgcgc ctcaaggtca tactaaaaag
     1321 gaacaagctt ctacatctat tctactagtt gaattaagta aaggaagttg ccaaatacat
     1381 ccgattaaag tagcagaggt ctattggaag acaaaggaaa tatcattgag gaaagcaaga
     1441 acgacaaaag aggtcattgt tcacattcgt cagcagcttt caaaagtaga ggatggattt
     1501 agtttaattg agataaaatt aaaagattat gaccatctca atacagatgt tttggaacgg
     1561 atacaatcag gagagctatt agactatttg ttagaggaat tctcagatag gataaatgat
     1621 ttctttattt ggcgtatttc tctcatagag aatacttttt atacgaagac cccacttgct
     1681 gcttctgcta aattgatgga acagttattt caatactatc agacacctca agatttccaa
     1741 caaattttaa atgaagtata tagtcatcaa gaagcggcta gaatattgag cgaactgccg
     1801 gaatatcaag aagaaacact tgaaaaagcc aagcagctgc ttaatcaaga ttttttattt
     1861 gaggaggatc aggaatgaag attttaagga tcgagattgc tgcttttgga aaatggcgtc
     1921 aaaaaagttt tgacttttat tcaggcaatc agctgatcta tgggggaaac gaagcaggaa
     1981 aatcaacgat ttatcaattt attcaagcaa tattatttgg tttcccttca aaaggccgta
     2041 aaaaaaagga ttatacacca aaagacggct cagcctatgg cggcaaaata tggcttaaac
     2101 atcctgtata tggtgaattt gctgtcgagc gctacaagca gcaaaatcgc ggaaaatcga
     2161 aggtgtggct gggggatcaa gtgggaagtg atgagttact agaaaaactg atcgcacctt
     2221 tgacgaaaga gttattccag caggtattta ctttccagca agagcagttg atggaactag
     2281 atcatttgag agaaaaagag ctacatgatg cgctgatttc tttaggcatc acgggcagtt
     2341 ctcaagtatt ccaaaagcgg caagaatatt atcagaaagc acaacaatta ttcaagaaaa
     2401 aaggccaaaa attactaatc aatcaaaaat taaatgaatg gaaagagctg caagaaaaaa
     2461 ttcatcaaaa acaagaacag gaagcttatt ttggcaagtt gatccaacaa gaacaggaat
     2521 acgagtcgca acagcagcag ttattggcac aattaaaaga ggcgaacaaa caattttcat
     2581 tagccagaca gcaagaactt aatttttctt tatatgaaga atggcaatct ctcaaaaaaa
     2641 acagcactac aggtcttcct gatgagcaac ttcttttaga tctggagtct ttctccaaac
     2701 ggtatcagca aacaaatgag cgtttggaag aattagaagc aacactggaa aagcatagcg
     2761 ggatggatca gcagtcagcc agatattatt tttatttaga gcaggagcag gaactaaaag
     2821 atctccagca acttcaattt acagcggaac aattaaacga agaatggcaa agattacaaa
     2881 ttgagactag agaaacaaat tcagaattga agattttgga acaaaggtgg cattggcaga
     2941 aaaatccgtt accggaagaa tttttatatg aagaagaatg gaagcatctt ctggctgaag
     3001 aaagaaggat tgctgatcta gaaagccaga tttctgtgcg tctccaaata gctaaagaac
     3061 agcaagagat gattgaagca gaagtgaatg acttggaaga gacctatcca gcattattac
     3121 aaaatcagtc agagatagca ccagtctctt catctaaacc gtatatgctg atggctggcg
     3181 gagcaataat cattgcaggt atatttgtac caatgccctt gaaagtgatc cttttactaa
     3241 ttggtgcagg catgattggc tatttcttct acaaaaggca accaaaaatt aaaactaaaa
     3301 atgatttctc tgagataaaa ggaatgtggc aagaaaaact aggacaactt gatctagcct
     3361 tagctcaatt agctgaagca gaagaaaaac aaaagagaac tggagaagaa aaacagattc
     3421 ttcagcaaga agtatatcgt tttgcggatc atcggcactt aggaaagatg gatacgctca
     3481 aactcatgaa agaatatgga caggaagtca tagattatca gaaattatat ggcgtatttg
     3541 agcggaaaca taaaagagaa caggaaatac aagaaaaact acgacaaatc gaccaaaaat
     3601 ttgagtttgt taaagaatgg ttaccgatac aagataagac aatttcagaa aaaatggtat
     3661 tgctttcccg ttttatacaa gagatggaac agatgaaatt tgccagaagc tatcagcaaa
     3721 atactttatt gaagcaagaa atcaatcaat tgaaaaagaa acaaaaagta tggatacaag
     3781 aatacagcga acaattgact caagcaggct tatcttatcc ttcggaagta cctgtctttt
     3841 tacaaaaagc caaacaagcc gaacagcaga tgaaaagata caatgaatta tcggaaatgc
     3901 ttactccact atttcctgaa gagctgacaa aagagaaatt atctgtaaga ctgcgccaac
     3961 tgaaagaaaa gcaatcccaa ctccagcaag aagaaaaaga agcaagtgaa aatcggcaac
     4021 gactccaact tcaagtagaa gagttgcaaa gagacggcac attggacgaa ctttatcaac
     4081 aagctagccg gcaaaaagca aaattacagg aattactgga ggaatgggga gcacttgaaa
     4141 tcgccgggac attcttaggt gatctttcaa cagagatgag tgaaagacaa ttaccccagt
     4201 tgttaagagc agcaggtgat tatttaggta ttttaacaaa aggaaattat cagaagatat
     4261 tactgaaaaa tgatacactg actttaacag acggatatac agaatttccc atctatgagt
     4321 tatctacagg tacgaaagat cagctgatta tggcgatgag atttgcttac ctctcattag
     4381 aagctaaccg ttcgatatgc ccgatcatca ttgatgatgg ctggcttcat tatgatcatc
     4441 agcgaaagta tcaattagct gaacttttag ctaaatttgg agaaaagtat caaataattt
     4501 gtttttcttc tgataaagaa atggtaagct attaccatga gatgaaccaa atcgtaaaac
     4561 aattagaagg agcaaataat gaaaaaaatt cgtgatttag taatcgatga gcagtttgaa
     4621 caatttgtcc tgatcaaaag tgcagatgta agaatcgcta aaaacggaaa aaaatttatt
     4681 gcttttactt tccaagatac ttcaggtact atcgatggta aatactggga tgcttcggag
     4741 gaggaaatca ataaatttac agccggcaca gtcgtttttc taaacggtaa aagagaggtt
     4801 tatcaaggaa accctcaagt aaagattctt catatgcgct taacaagggt agatgagcca
     4861 ggagagccta gtttatacat ggaaagagcc cctttgaaga aagaagaaat ggaagaagaa
     4921 atcaatcaat tgttgtttga gatcaccaat gcgcattgga atcgtattgt tcgctatcta
     4981 cttaataaat atcaaaaaca attttttgag tttccggcag caaaaagaaa ccatcatgca
     5041 tttgccagtg gattagctta tcatactttg acaatgcttc acttagggaa atcaatcgcc
     5101 gatgaatatc ctgagctgaa tcgagcattg ttgtatgctg gtatcatttt gcatgattta
     5161 ggaaaggtga ttgaactttc cggtcctatg tcgacagaat atacgcttgc aggaaatttg
     5221 atcggtcatc tggtattgat tgacgaagaa ataactaaag cctgtcttgc gttgaaaatc
     5281 agcgaagctg acgaagatgt tcttgtatta cgtcatatgg ttttagcaca ccatggctta
     5341 ttagaatatg gctcacctgt acgtccaaga ataatggaag cagaaatatt acatcaaatc
     5401 gacaatatcg atgcttcgat gcagatgatg ttgacttcga ttcgtcaaac agaaccagga
     5461 aaatacaccg atcggatttt cggtatggac aaccgaagtt tctatgtacc aaaagattta
     5521 tagcaaaaat ttattttttt cattcaaaac aaaaaaagcg agacataagt cttcgctttt
     5581 tttgttttct tttattttga tgaatcagtt gtttcttcag ttgaagattc ttttgttgaa
     5641 tcagttgatt ttgtgtcaga actctttgtt gaagctgtag tttcacttga gtctttcgta
     5701 cttgatgaag ttgttgtgaa tgcactcaac acattttcaa aggcatcatc tttgattttt
     5761 acatttgcat cttttaattc ttcaccgatt actttagttg tgaatgcatt gtcacttagt
     5821 ttagtttcag tagcgatatc ttttagttga tctttgtatt tatccatgtc attgccttta
     5881 ttttggtttt tcaccatttt gacaacgtag tattctgtcg cataagaagt aggatttgtt
     5941 gtagtaataa catcggagat ctcaccgtct ttcaatttga aagctgcttc ttttacttct
     6001 gcaggaatag ttgttgtcgt tgagtcgaat ttaactttac cgccatcttc ttttgtctct
     6061 gtatcagttg atttgtcttt ggctaattta gagaaatcat ctccatcatc cgctgatttt
     6121 ttcacatctt ttgcttcatc ttcacttgat aatttgatga tttgtgcttc tacttctgga
     6181 tggaaagatt tccaagcagt tttcaaatcg tcatctgtaa tatctacgtg agctttcaag
     6241 ccagcttcaa aagctaaatt gttacgaata tattctttat aagtatcttt cgtatatcca
     6301 gcagcttcta attgtgattc aaaagtatca cctaatgatt tcgcttgttt gtcgtattct
     6361 gcatccactt gcttatcggt tactttatcg ccatatttgt tattgaatac tttataaatg
     6421 atcatccgtt gtactaagga ttggttcgat gattcaagtt ttgcttcatc atagaaatca
     6481 gatacagtga tcgttccgcc tttcatcgtt gcgatatctt tattagtatc gcctgaacaa
     6541 gctgctagag ttaaaacagc caacgcacta gtagctgcca aaataattga tttttttttc
     6601 attaaaattt gcactcctgt aattagttat tatttttata tattaactat accatatccc
     6661 atttccttta aaaacgttgt cgtggtagtt tcaaacaaaa ttcaaaattt catcacattc
     6721 ctaggaaata cgcaaacttt tctcacttat atagttaaat ctgttgagaa aataatcatt
     6781 attatatttg ttcaggatac tctggcagat cagcttttag tttttctact tgttcattga
     6841 tttgtgtgat acgtggttcg gcttggaatt taaaatcttc gatatctttt ttgattgctt
     6901 cttggaaagc aggaatcaca gattcagctg tttcttttgt ttcgatcaat gcgcttttaa
     6961 aatgattcag gctgttattt aaatcatttg tcagagttgt tgcttcttcc aattcttcga
     7021 tcagtttctt tcttgtttca ttcccgctcc tgggcgccaa taacagacct ccgacagtgc
     7081 cagcagcagc tccaaataac agtcctttga aaaagttttt aatcatcgca ggacacctgc
     7141 tttacgattg tttcttgaat agcttttagt tcttccgatc cataactatc ttgatgattg
     7201 acaaagtgaa tagaaaaatc atcctctttg ctgtagcgag ggactaaatg gatatgtgaa
     7261 tgaaaaacag attgataggc tagttcacgg ttgttattca agatattaag acctttcatt
     7321 tctggaaaag ctttttccaa tgcttgagca atcttaggca cacgcgaaaa aacctctcct
     7381 gcaagttcgg gatcatattc aaaaatatct gctacatgac gtttaggcac aactaatgtg
     7441 tgtcctttcg ttgcttgaga gatgtctaaa aaagcataga ccttgtcgtc ttcatatatt
     7501 ttatagcttg ggatttcttg attgatgatt ttgcaaaaaa tacagttttc catataaaac
     7561 atcctttcta taaagaataa atacttcagc ttaactttac cataagttaa agctattgta
     7621 aggaaaaaaa caaaataccg tcattttctt cgtggtataa taaataaaaa taaatggcgg
     7681 aggaaaaaga atgagtttaa ttatcgatca tgtcacaggg gggtacggac atctccctgt
     7741 gttaaaagat atcagtttcg atgtacagcc tggagagata gtagggttga ttggactgaa
     7801 cggagcaggg aaaagtacga ccatcaagca cattatcggt ctgctggaag cttcaaaagg
     7861 aacgatagaa atcaatcatc tatccttaaa gaaaaatacg gaagaatatc gcaaacaaat
     7921 tgggtatatt ccagagactc cttctttata tgaagaattg acattgagag aacatatcga
     7981 agtcactgcg atggcctacg atatacctat ggatatagca atgaaaagag cagaaaagtt
     8041 gttggagact tttcggttat ctaatcgtct tgactggttc ccagctaatt tttcaaaggg
     8101 aatgaaacag aaagtgatga tcttatgcgc ctttttgatc gagccttctt tatatatcat
     8161 cgacgaacca tttttaggct tggatccact agctatcaat gcattgcttg aattgatggt
     8221 agaaatgaaa aaagaaggag ccggtatttt gatgtctacg catattttag ctactgcaga
     8281 aaaatattgt gatcgttttg tggtcttaca taacggagag atacgagcag acggttctct
     8341 tgagatgttg agaaaagaat ttcagctgcc ggattcttct ttggacgaaa tctatattgc
     8401 tttaacaaag gagcaggcac atgagtagtt tttttcaaaa acgtttagca agacatcaaa
     8461 agaaaatgat gaaatatatg cgttatgtat tgaacgatca ttttgtttta gtttgtcttt
     8521 tcttgattgg cggtgtggga ttatattatt ctaattggtt gaaaaccctg cagccaccgt
     8581 ttgcaattgg agggctcata atctctgtat tttggatgct ctgtttattc gtgggtaaga
     8641 tggctacttt tgcagaggca gcagatattg tttttttact tcctaaagaa aaagaaatgc
     8701 aaacctattt ggaacgagct tttcgctatt cttgcatttt tccgttgatc tttttgatat
     8761 tggcatgcgg attggccatg ccattagtgg tggttagtac ggagcaatcc ttttcgatgt
     8821 ttttcgtcta tctgatcata ttatggagtt tgaaattcag tcatctgcaa atcaagagag
     8881 cagaattgtt tcgactagat caacaagtta ttcgttattg gaaattaggc tggttttttt
     8941 cttctctgat ctttttgttt ctatcatttt attggctaat atgggcagga atagcaggtg
     9001 cattagtaca ggcaagtcta tattactggt ttttgtggaa gcaacaagaa aaaggattgg
     9061 attgggagaa aatggtgcat gtagaagaac ataggttgca tcagatctat caatttatca
     9121 atttattcac agatgttcct gaaattacgg caaagatcaa aagaagacgc tatttggatc
     9181 ctatattgac tcatattaaa agagaatcaa aaaacactta tctttacttg tttgcaagaa
     9241 gattggcaag aggttctgat tttagtggtc tttatttccg attagtaatt ataggtgggt
     9301 tattgattgc cggagtagtc gacttttatt ttagtctgat tatcggtttc ttatttttat
     9361 atttgatcgg tttccaactg ttacctttag cgaaccagtt ccgctatatg gttctcgttc
     9421 agctttatcc aatccataag aatcagaaga agcaggcgat tcagtatcta ctcacatggg
     9481 tattaattat cgcgtcggtt atttttggtt tgattgctgc agtcgtatta gatgggcagc
     9541 agcgatgggt tccgctagcg gcttatttga ttgttactat gctctttgtt aaactatatg
     9601 taccaactcg tttgaagaag atgtcagact gaaaacaaag ctgtgtaggg taaaaatgag
     9661 aatgaaaaat agagcgttga cagtaagaat gctgttcgtt ctattttttt tgagaaaagc
     9721 tggttttttg aatggcaatt aaatgaaaaa gaacgtatat gtgcttgacg tgtagtgttc
     9781 aggcgactag aataataagt actgcaaaac aaagagtaag aggacgggag aatgcatgat
     9841 ggagtttcag ttagaccaag agtggcggtt acgtcccatc aaaggcgaca caggaaaaac
     9901 atacataggc atgcgtgatg aggacaaagt ctttattaaa cgaaacacga ctccgatgtt
     9961 ggctgctctt tcaagggaag gaattacacc aaaacttgtt tggacaaaac gaacggggaa
    10021 tggcgatact ttgacagcac aagagtggct ggaaggtcgc ttgctgactc ctaatgagat
    10081 tggtcaaaga aacgacgtga ttgatgtttt gtaccattta catcattcaa attctcttaa
    10141 aaccatgctg acccgtatcg gtggtcgtgt catgacgccg gagaagatgc tgcaaaaata
    10201 tgaaaaagaa ttaccggaag aactccagaa aaatcaattt ctgcaaattg tccatcgttt
    10261 tcttcgtgag cagatcccgt caatagataa ggaaaactta tcagtagtac acggagatgt
    10321 aaaccatcgt aattggttag tatgtaaaaa ttatttatat ctagtcgatt gggattcaat
    10381 catgtttgca gaccctgcgg tggatattgc aacgatttta ggaaattatg tgccactgtc
    10441 tagctggaat cagtggctga tcagttatgg catacgcccg actgacgaaa tgcttgaaaa
    10501 atttcattgg tatgcggcca tgaatttgct ccaagaaatc accagatatt gcaaacgtga
    10561 agatcatcga cggatgaacg aagaaatttt acaattaaaa cgaattttca gtggctgagg
    10621 tttggcccaa actctgcgga gatttggcca gaccttttta attagaggaa aggaatggag
    10681 ataaataatg cgtgtccgaa atcgtccggg agcagcggaa atgttagcgg ctcacccgaa
    10741 ttttgtaatt agtgatccaa cattgtggaa gggaaaatgg aatgaattgt ttgaaaacga
    10801 tcatccaatc cacattgaga ttggtatggg gaaaggccag ttcatcactg gaatggcaaa
    10861 agcccatcct gaaatcaatt atattggagt agaaatgcag gtcagtgttg tttcgattgc
    10921 actagacaag ttgatcgaac aaccgcttcc taacctgaaa cttctacatg tggatggatc
    10981 agcgttgaca gagtatttcg ctgacagcga agtagatcaa atctatctga atttttccga
    11041 tccatggcca aaaaaacgtc atgaaaaacg gcgattgaca tataagactt ttcttgctgt
    11101 agatgaacag attcttcgtc caaatggaga aatccatttc aaaacagata atcaaggatt
    11161 gtttgagtat tctctggcaa gtttttctca atatggcatg atcctcaaac aggtatggtt
    11221 agatttacat caaagtcaat ttgaaggcaa tattatgacg gagtatgaag aaaaattttc
    11281 ttcaaaaggt caacgtattt atcgagtaga agctcaattt caagataaga ccaaaaagaa
    11341 aagtctgtga aactaacttt ctgctttcat aaaaacatta atatccataa ataaatctca
    11401 gaaaatgaac attacagatg taatgtattt tctgggattt atttgattta aaatgtattt
    11461 atttcatcgg tttttccaca aggtggaatc aattggaagc gagaaaatga aaataatcct
    11521 ttgtcggtga tcttcaattc gggtatgaca gataatgtta agaaagaaag tgtcaaaaaa
    11581 ggatcaaagg ttttagctgc tcctagttga tgtgctttag ttattaattc attcaactgt
    11641 tcatagacga tcgaaaaagg ttcctgtgtc atgattccgc ctataggaag agaaacacaa
    11701 gctatttcct gctgtttgtt gacaacggtc atcccgccac ctttttgaat caaaagatta
    11761 gcagcataca gcatatcctc gtcgtttgtc ccaacgacaa ctagattatg cgagtcatgg
    11821 gcaatcgttg tcgctatcgc tccttgtttc agctgaaatc ctttcactat gcctaatcca
    11881 atgtttcccg tttgatggtg acgctcgatc accgcaatct ttaattggtc tttttcgatt
    11941 gaaggaatga atcgttcgcc ttgtcggtcc actttttcta ccaaatcttt agtcactaga
    12001 ctattcggga taatttcgat cacatgggct tgtgaagaag aaagaagcag ctctaacgaa
    12061 tgtggattta atggttgagc gtttagtttt ggcagacaac acgtatatga atttgaatga
    12121 tcagcaaata aatggtcttg gagttgccca gattgaacaa ctaatgttcc cttatagaaa
    12181 acttcatgaa ttggcagctt ggtaagatcg tctgttaaga agaaatcagc ttgataccca
    12241 ggggcaatcg cacctagtgt tttcaaatca aaacattctg ctgcattcaa tgttgccatc
    12301 tgaatggctt gtaatggatc gattccaagt gcaatagctt gacggacgat atgatcgatt
    12361 gatccttctt ttactaaatc attgatcagt ttatcatcgg tgacaaagaa gcatcttctt
    12421 gaattttcag gtgtgatcgc cggtagtagc gatgccagat cttttgcaac ggtcccttct
    12481 cgaaccatta aatacatacc aagatccaaa cgttctttag cttcttttgc agttgtagct
    12541 tcatgatccg tccgaatacc tgctgcagga taaacattta actcttccat gccgatacca
    12601 gctgcatgcc cgtcaatttt tttcttatgc tggtgcatca attggatctt ttctaggatg
    12661 tctgtttcat tatttgctac tgcttgatag ttcatgactt cagctaagcc taaaacttcg
    12721 ggtttttgta aaaaaggggc taattttttt gcatttaaat ttgcaccatt ggtctcaaaa
    12781 ggggttacag gtacacaaga aggtagcata aaaaagatat tcatgagtgt ttgtcttgca
    12841 tcttctatca taaactcaag acctgtactc ccagcaatgt ttcctatttc atggggatct
    12901 gtcactacag tagtaactcc atgaagcaag gatacttttg aaaattcgct tggtgataaa
    12961 agtgagcttt cgatatgcac atggccatcg atgaatcctg gcactagata ttgcccatta
    13021 gcattatgta cgctttttcc ttcgtattca ccgattccta cgatcattcc ttcgcagatt
    13081 gctacatctt tttccatgat ttctttcgta aagacgttga cgatcttagc atgcttgatc
    13141 actaggtcag caggcttttt tttacttgct acttcgatat atttccttaa tgttgtttcc
    13201 aatggagtgc ctcccaagat aaatttttat aagtataaca taaattcggc ttttgccaat
    13261 atttttttgt aaattcattt tatttgaaac aaaaagatca aaaagacgta tgttttttgt
    13321 ggaggggaga ataatggaaa taaaacgatt atcggatcaa atgataagtg aaagttttgc
    13381 caaacatgcc caagacgtat atatattgcc agcttcagaa gtattttaca tctattttcg
    13441 ctatggaaaa aaacgaacat tctggaagaa agtctcttcg gcagatggac agcaactgat
    13501 ctcaagattt aagtatttgg gacagatgga tatcggagaa aaaagaaaag ctcagttagg
    13561 tgcatttact tattctttag aaaaacagga agttcgtctg cggttgtctt ctgtaggaga
    13621 ttaccttcaa cgagagagtt tagtgattcg gctattatac ggtacttttg aaacacccca
    13681 tcgttgcttt agttctcgag attttgcaac tattttacag aaaaccgtca gacgtggatt
    13741 ataccttttc agcggaccag tcggatcagg caagacttct ctaatgtatc ggttggcttt
    13801 ggaagaaaaa ttgcaagtga ttactatcga agatcctgta gagatagaag aaccgttttt
    13861 tctgcagcta cagatcaatc cgaaaatagc acagacttat gatacacttt tgaaactcgc
    13921 attacggcat cgtccagact tgctaatcat tggcgagata agagatgaat caactgcaca
    13981 tgctgcggta agagcagcac taacagggca ccgggttttt gcaaccgtac atgcacgcaa
    14041 tcttgaaggt acattggtca gaatccatga actgacaaag aaaaaagaag agctatctga
    14101 atgtttagct ggtgtcattt atcaagaact tttggaagat gccaatcacc atccttctgt
    14161 tttatggagt tatcaattta tggaggaaga atcagtaaaa aaatcatggg aggaaagtta
    14221 tgaagaagct caggaaaata tttggcagtc agaactattc taaaggatta acaagaagcc
    14281 agcaaaacca gtttgcacaa accatgggag aactgctttt atctggtttt agtctgcaag
    14341 aatcccttta tttattactc acgatccgtt cgtttccacc tgttattttg gcaaatatcc
    14401 aacatcagct aaaggaaggg caagcgttta gtgaggtact ttcgcaaata ggatatggag
    14461 cagagcaact ggtacagatc gagttagctg aacgtcatgg gaatctgccg acaactttgt
    14521 tggaaattgc caaacagttt cggcttgttg aaggatttcg caatgatttg aaaaagctgc
    14581 ttgcatatcc gatgctattg ctcacctttt tgctaggtat cttgtttagt atgcggttgt
    14641 tcgttttgcc tcagcttatt ggttcaggaa tggtcatgca agagcattgg ggtattcagc
    14701 tgatacaagt ctttcattgg tatgttctac tattcatcct agcggttact ctattggctt
    14761 tactagtaag gtatcgtctg atgaagttgt ctcctgttga tcagagtgaa tggataagca
    14821 gacaattatt gattggaccg atatattcct tgtatcaatc ggcttatttg tcactagaga
    14881 ttggaaagct ttttcatgaa ggtttcgaat taaggcaaat catagtctgc ttaaaggaaa
    14941 cgaaacaaga aagtctgatt cagcatcttg cctctaagtt gattcaagga ttggaggcag
    15001 gtatgtcatt ggctgagcag ttccagaatt atcgttttct atcagaagaa ttcagtaaaa
    15061 ttgttttaca gggagaggca aagggaagtt taggtaaaga actgattttt tacagcgaat
    15121 ggacaagaaa acagcttttc aaacgtcttc atcactggat gcattttatt cagccaatca
    15181 tttttttggg cgtagcggct ttgattttat tagtttatgc cgctgtttta ttacctgttt
    15241 acggaagcat agaggaggtt ttgccatgaa aaagagaaaa atatcttatt atagcggttt
    15301 tacattgatc gaaatgctaa tcgtcttact gatcatcagt gtattagtgt tgttgttcgt
    15361 ccctaatttg tcgcgttata ggaatcatgt agaccaagaa agtcgtgaag cgatcatcca
    15421 gttggtagac acacaaaaag agctctatgc gcttcaaaat aatggaagag ttcctactgt
    15481 ggaggaattg ttgaatgaag gctatatcaa acgagaacat gcagaaatct atcagcggcc
    15541 ttgaaggtaa ggttttagca tcaggatata cgttgtttga atcactctta ctgattttat
    15601 tacttacaac gatcctttct tttccagttc tcgcattttc tgcatggaag aatgaattgg
    15661 cagtctatca atttttcggc caatttgaaa aacgaatcta ttctacgcaa aaaatcgcta
    15721 ttgtgaatca gatccaaaca ggcttttatt ggaatagtga agagaatcaa atcatttttc
    15781 atgttccgaa tccagataaa ataaattggg aagtattaga tattcctgat gagattacct
    15841 tgaaacgcca tgcttctatt acttttgctg ctagaaccgg gaatgaaagt tcactaaaag
    15901 cttatcaatt ttattgggaa gaaaaagagc ggacgattac gtatcaattc cagataggga
    15961 gtggtcgcta tacaaaaaga attgaataaa acaggatttg ttttgttgga gtcccttaca
    16021 gcgcttatga tcgcttctgt ctgtgtctac ttagtgagct ttggccagct gtttttgctt
    16081 caggcagaaa agaaaagtgg ggaggaactg ctatttattc gaatgattta tgaagatatc
    16141 aagacgcagc ggatgtatca caaaagacca gagcgaaggg aagaaatgat cccatacgat
    16201 atccaaattg tcgataataa tggagtaatc gaaaaagtgt ttgtcaatat ggagaaagaa
    16261 gcgatcagta ttgaaaaaac aagtaattcc agcatttacg ctgattgaat gtctaattgg
    16321 actgatgata ttgagcagtc tattgctcac attcaatctt ttgattcagc aaaccatcca
    16381 agtaagtgaa tttttgcagc gtaaagatca aaaagagtgg ctaatatttt tagcacaatt
    16441 agaagaagaa ctcaagagca gtcacaatgt ctctgtgaaa aatcagcaac tgcactacac
    16501 gatcgaggat aaacagtata tcatcgaaag atatcaggca atgatacgta aaagaagaac
    16561 ttccggcgga catcaaccta tgttaacaag tatcactgag cttcaattat tggaaaaaga
    16621 acacacaatt tctttttatg tacactttga aaatggagaa gatggatatg ccatatggac
    16681 aaaaaacgat cagtagaaaa tgttataagg gctctatttt attttctgtc ttgcttttat
    16741 ttcttttatg tacgttttta ttgatggcag cattagaaag ctaccgtttg tcaatggatc
    16801 ttaatatacg aatcaaaaat tattatatag caaaaataat gaagcagatg gctttatcgg
    16861 aacagtctac gcttgccgaa agttcggaag gagtttttta ctacacaact ggatctgtaa
    16921 catatcagtg ggtacaacag tccctattct tagaggtgga agtctcaccg tttatctttc
    16981 ggttcacaga agaagtaaaa aatgatacag atactagtac agaatgatgg aatgttcgtg
    17041 aaaacgccct cttttctgtt ataacactgt ttttaataaa aaaatgtttg ccgtttcaca
    17101 aataaaaaac agtgaaaacg attgaaattt tgtcaattgt tcgatagaat taaacaggtg
    17161 aaaaaattgt tataatatat ctagaaatga ggtgcaccat gttaccagaa aaaatggaac
    17221 aagggtttaa tcaaaacctg gaagctattc agcttttaca gaatgcattg ggaacttcct
    17281 ttttagaagc ctatgtcgaa aatgcagaaa atttaataga tgattatcaa gtacgtgtgg
    17341 tggatggagt gccgacaaaa gagacaacgc aacggatcac agctctttat gaagagttga
    17401 agaaactttc tttcgagcca gaagaatggc gcagactgtc tcaattactt ttattgaagg
    17461 gcagccagac agaacatttg caggcgaacc atcaactcac tcctgatagt atcggttttt
    17521 tatttgtttt tcttattgaa caactataca caaataaaaa agcaccggtt aagattctgg
    17581 atattgcggc aggaatgggc aacttgttat taacagttct tttaaaccta tcgaatgcgg
    17641 ggtaccaaac agaaggaatt ggtgtggata ttgatgatac attgttggct gtcgcagctt
    17701 ctacaagtga tttgacacaa gcaaatgttc aatactttca tcaagacggg ttgcaggaat
    17761 tattaataga tcctgtcgat tttgcaatca gcgatttgcc aatcggttat tatccgaatg
    17821 acgaaaaagc aaaagagttt ttaacaagta ccgaagaagg ccatagttat gcacatcatt
    17881 tattgctaga gcaatcaatg aaatatgtga agccggatgg ttttggtttg tttttgatgc
    17941 cgagtggatt tttagaaacg gatcaaagtg aagaaatcaa aaaatggttt aaagaagaag
    18001 gttatcttca aggaatgatt caattgccag atgaattgtt ccgtaataaa caatcgcaaa
    18061 aaagtatttt gatcttacag aaaaaaggac cacaagcaca acaagtaaaa gaagtcttgc
    18121 ttgtgaaact tgcttcatta aaagaaccag agaaggtgac ggaatttttc aacgagttta
    18181 aaaactggaa atcatctgct ctgtaatcat tcaacgagaa ggagagtcat atgtctaaaa
    18241 caattgcaat caatgctggg agttcaagtt tgaaatggca attatatcaa atgccagaag
    18301 aaaccgtagt tgcgaaaggg atcgtcgaac gtatcggttt acaagattct atttttacca
    18361 tcaagtacgg cgataatcaa aaattcgaac aaatcatgga tatcgaaaac cacgaagtag
    18421 cagtaaaaat gctgttagat cagttgattg aactgcaaat tttaagctct tatgatgaaa
    18481 tcaccggagt agggcaccgt gttgttcacg gaggagaaac ttatggggat tcagtagtga
    18541 tcgatgagaa agtaatggac cgcattgctg aattagcaga atttgctcct ttgcataatc
    18601 ctgcaaactt aatggggatc aaagcgttta aaaagatttt acctgacatt ttgagtgtgg
    18661 ctgtattcga tacatctttc cactcaacaa tgccaaagca aaattattta tatagcttac
    18721 cggtgtctta ttatgaagat ttcaaagtaa gaaaatatgg cttccatggc acaagtcatc
    18781 gttatgtgtc ggaacgtgcg gcaaaattat tgaacaaacc gatcgaagaa ctaaaaatca
    18841 tcacttgcca cttaggaaat ggtgtatcga tcacagcagt cgatggaggt aaatccatgg
    18901 atacttctat gggattcaca ccattagctg gggtgacaat gggtacacgt tcaggtgata
    18961 tcgatccagc tgttctccca tatttaatgg aaaaattaga gattgatatt gatgaaatga
    19021 tcaatgtttt gaacaagaaa tctggtctgc taggcctaac aggattgtct agtgatatgc
    19081 gtgatcttga aaagaactat gaaaaagaac atattcgttt agcttatgac attttcgtgg
    19141 accgcatccg aaaatacatc ggcggttatg tgacaacgat gaatggtgtt gatgcaattg
    19201 tctttactgc tggtatcggc gagaatgacg gacatgtacg tagtgaaatc atcaaaggta
    19261 tgtcttggtt tggttgtgaa atcgatccag cgttgaatga attgcgtggt gaagaagtag
    19321 atatctctac aaaagaatca aaagtaagag tattagtgat cccaacagat gaggaactaa
    19381 tgattgcccg tgatgtggag cgtctgcgcg gctaagccaa agcattcgtt aggataaaag
    19441 atgaaacgca tgcaagaggg aattcttgca tgtgtttttt ttgcggagaa gaccgcaaaa
    19501 tggtaattta acttcaattt cccttctttt ttacaggatc ttcttaggga attttgcttg
    19561 gaaaagtggt caaaagggaa aagactggca ttttatttat tgattttcag gaaaatccca
    19621 atattttatt ttcattcatc tttataaggg acattacata tgtaaaaaga aaaaacattt
    19681 ttagatcagc ctcgtgggct gtcgacttta ttcttcgctt tatgtttagc tagaaaaccg
    19741 atccgaaaca ttcattgatc gaataataaa aacttgattt tccacctgta ctttgcctga
    19801 atcgtgaaag cacaggtatg aaaatcaagt tttttgttgc gaaaaaacgt cctagtcaat
    19861 cagttggccg agcggctggc ggctgatctg ctcctgatca ataacgagtg cttcttggaa
    19921 gtgttgctgc aaaaagtagc ggtcatggct gactgctagg agcgttcctt gatagtcttg
    19981 cagcatttct tcaatttctt ctcgagcata gctgtcgagg tgattggttg gttcatccaa
    20041 aatcaggacg ttgatttttt ctaaaaagag tttcagcaac gccaaacgga ttttttcacc
    20101 accggataaa tctttgatcc gtttacccac atcgctttga taaaagccac ttttggctaa
    20161 ttgccgtcga gcgacttctt cgtttccagt caatgtgatg gcgtaagcca gcaaacggct
    20221 atcgggatcg gcaaactgca gctgctgcgg caaataaccg atttgcaagc tactacctaa
    20281 tttgatggtg ccaccgtaag gcttttcctc accgagtaat aatttgagta aggttgattt
    20341 tcctgcacca ttgtcgccga tgatggctaa atgatctcca cgatagactg caaaactgct
    20401 gtctttgaat agtacttgat cagcgtactg ctggtgcaag ttttgaatga tgaaaacttc
    20461 tttgccagat tttccaccat tagacaagct ttgcagccga tttttcgggg gaggtggcgg
    20521 tttgacgagt gtcagtttcg ccaggcggcg ttccagttct ttggcttttt tgaagaagga
    20581 ctcattgtct ccttcatgtg cccactgacg aaaacgacga atcatgactt tcaaccgctg
    20641 gacttctttt tgctgcagtt cgtaattttt tgtcagctca gccaagcgag cttctttcaa
    20701 gactcgaaaa cggctgtagt ttccgggata ctcaatcaat gcaccttctt ccagttccca
    20761 tgtttttgtg gtgacttgat cgagaaattg ccgatcatga gaaacgacca gaaccgattt
    20821 ttttgtattc tgtaaatagt tttccaacca gcgaatactg ttaagatcta aatgattggt
    20881 cggttcatcg agtaaaagta aatcggcatc ggctgctaaa attttggcta aagcgacttt
    20941 aactcgttcg cctccgctaa gttctgttaa ccgtgcttcc tgacggtgtg gaatccctaa
    21001 gccttgcaag atcgctgcaa tccggtcttc taatacgtaa ccgcctagtt cttcaaagcg
    21061 atgctgcagc tcaccgtaac gggccagaca cttctctaag tttgtatctg gttctgtcat
    21121 caatgcttcg tagcgactca tttgctgttt gaggtcctgt atctcagcgg cagaatggag
    21181 caagtagtgg ttgacggtcg cttcggaaac tgtaagttct tgttcgaccg tcccgatctt
    21241 taagcctttt ttgcgactga taacaccgct gtcgacacct tctctgccgg tcaagatttg
    21301 taaaaaggtt gatttgccag ttccgttgat accgactaag gcaatttttt caccagcttc
    21361 aatttgacag gataaatcct caaataatgg attcccgccg attgtttttt taatagaaat
    21421 tgcttgtatg atcatgatct ctctccttga gaccactcaa aaaggccatg gttatccctg
    21481 gcctgtgtta tttaaactca gattatcatg agaaaaatac cttgagatgg ccattgattt
    21541 tacgtgaatt caactgtttt ggacaaactt gtcccgtcaa cagcaactgt aaaaccaatt
    21601 gcatggctaa atgcgatatg gtatcgttgg taattttcat tacttgttat tttagtcatg
    21661 cgcatcatag tattttccct ccttcgtgta gttgtctttt ccagtgtacc tgaaagtgtc
    21721 agaaagttca actagcgctg acatttactt aatatttatc gatgtgtaat tctgtcagag
    21781 agcttttttt atctgactga gggtaatgtc tcgtggacca catcattgta ttctgagcta
    21841 aaccagcgtt caggcgaaat catatggtgt ttgataaaaa actttgtcta catgacttcc
    21901 ttttgccatt tgcttaatac cgttgcatta aacagctctt ctctttggaa atctcaccaa
    21961 catcgcgttc aggagtatcg ttgtggttgc aatggcaagc ggtagccttt catcaattct
    22021 tgaatgattt accgaatgta gcacttgcct tctcgcggta cccgaacccg aaataaaatt
    22081 ttagtttggc gataaaatcg gtatccgcca tcgatacttc atattttttc gtatcagatt
    22141 catcggaaac ttccacattg acgaaccaat aagctttggc atgcttaggt cgttttaaac
    22201 cacaccaggg cgactaatcg ccctggtgtt cacttttttt attttacttg aactcattga
    22261 ctgttttccc tttattttaa acattataaa gaccgatgag tgcttgccgc gtcttttgtt
    22321 tttatcacgg ttttttgtgc aaaaaaacct gccgatagtt ggcaggtcgt ctacgtgtta
    22381 agctttggtg tggtctttag ctggcaattg gttttctaaa tcagttcgga caacaagcac
    22441 atcgcaagct gcgtttctaa taacatattc agaaacagaa ccaataaata aacgttctac
    22501 agcattcaat cctgtggctc ctaacataat caaatctact tgattatctt ctgggatttc
    22561 tctggcaata atttgttttg gtgagccata ttctacgaca gaggttacat tattcaagcc
    22621 agctttcttc gcattgcttt cataatctgc caatgtttgc tttgccattt cagtagcttg
    22681 ttctgcaagc attccatcaa aggatgaaac ggtttggaaa gaacgtgtgt caatgacatg
    22741 tgctaataga agttctccgt tatttcggat agctacattg accgcctttt taaaagctaa
    22801 ttcagcttca tcagaaccat ctacggcaac catgattttt ttgtattgtt gtaacataag
    22861 cattcactcc tcaagtcaat cgtattgtac tcttttattg tagaacactt tagggaaaaa
    22921 gagaattatt tactgctttg gttcagcaat tttttttgca aatcggatac tgaaggtggc
    22981 agaaacaata attacgatca gtaaaaagac caaggcaata aagcgttgat tgaaaaaagc
    23041 tgttaaaata cccattacgc ctaaaacagc atatgcagca ccgtaaaatt gtaggaatcc
    23101 ttgattttta tcatttttgt tgatcaatac aaaaaagata tcctgtttat taaataaata
    23161 atagctaacg aataataata ctaaagtggc gataatcatt aaaatcctaa tcatgaacaa
    23221 atcctccaaa ataagcaaca aatagaaaaa gcaatttgcc aagagcaaat tgctttattc
    23281 tttaaaagat ggtaatctcc gttaatgccg ttgtttgtcc tgtagtattg atcccgcgaa
    23341 ttcaagcagg tgggtcccat ggtcgtagct tcgaactacc aaatgataag gcatgttacc
    23401 agcagtgaca caagacgggt cccaagatat caataaaaat gttcggtcaa cacacaaaga
    23461 ggacaattcg aacatcacag atttcccacc tctatagtag catatgaatt taaaaagagc
    23521 aatgagagag ctcgcagaaa cagaaccgtt ttcttgtcta gttttttgga ggatgctttt
    23581 tccactcttt tatacggtaa tactgttgac ccagagcctg ttcatatttc cctgtatctt
    23641 ttggctgata atactgagca ttcttcagtt tatctggtaa atattgctga ttgacccaag
    23701 cctgatcaaa atgatgagga tactgataac ccacgccacg gttgagtgat ttcgcacctt
    23761 tgtaatggct atctctcaaa tgatcaggaa catctccagc tttgccctct cgaatatcag
    23821 caagtgctgc atctaatgcc atgtaggcag aattggattt tggcgacaag caaagatcga
    23881 cgacgacgtc tgctagtggg atacgtgcct caggcaatcc caatttttca gcagctaata
    23941 cagcgttcac tgtacgagcc gcagcagcag gattacctaa acctatatct tcataaccga
    24001 taaccatcag acgccggcaa atacttgcca gatcacctgc ctcaactaaa cgggcgagat
    24061 aatgaagtgc agcatccaca tcacttccac gaatcgattt ttgaaaagcc gaaataacgt
    24121 cataatgagc atctccattt ttgtcatggg taagagcttt tctttggaca cattcttcaa
    24181 tgatcgacag tgtaagatga atccttcctt ctttatctga aggagtcgat agtgtagcca
    24241 gttccagccc attcaatgca ctccgcaaat cgccgttcgt tgcacgcgaa aggtgaagaa
    24301 gagcatcttc gtctaactgg attgcttgtt gtccaaggcc gcgttcttta tcccttaaag
    24361 catgttcgac tgccaactgg atgtcttgtt ctgttaatgg tttgacttca aagatctgtg
    24421 tacggctgcg gatcgctgga ttgatggtaa tataaggatt ctcagtagtc gctccgatca
    24481 agatgattcg gccactttct aaatgaggaa gcagaaaatc ctgttttgtt ttatctaatc
    24541 ggtgtacttc atccaataag aggatcactg taccactcat ttttgcttct tccgctacaa
    24601 cttgtagatc cttttttgaa tctgtagcgg cgtttagcat gcgaaatgca taattagtag
    24661 agccggcgat cgcacttgcg atactcgttt ttccagtacc aggaggtcca tatagaatca
    24721 tggaagagag catgcgagca tcgaccatgc ggcgtatgat ttttccttcc ccgactagat
    24781 gctgctgacc aacgacttca tcgatcgttc gaggtctcat gcgataagcc aatggctgtt
    24841 gcataggatt ttcctccttc ttgtttttac gtcccttaat tatatcatat ttagcaagat
    24901 gcctagaaag aacagccttc tgttgttttg ttttcatagc ttatccaact aatattgtgt
    24961 atgataaagt aggtgagaaa aatgacggat aaacaaaaga tagatgaatt tttttggaat
    25021 aaaacgacgc cagaagtggc acgggattta ttggggatgt atttggagca tgacacacca
    25081 gaaggaagat tagctggata tattgtagac gcggaagcat acctaggtcc agaagacgag
    25141 gccgcccata gctatggttt aagaagaaca ccgcgtgtgc gggcaatgta tgaaaaacca
    25201 ggtacgatct acctttacac gatgcatact catcggattt tgaacatcat tactcagcca
    25261 gaagggattc ctcaaggagt aatgatacgt gcaatcgaac ccgccgcaat gattgatcaa
    25321 atgagtaaaa atcgtggcgg aaaaactggt ccagatatca gcaacggtcc gggaaaatta
    25381 gtggaagcat tagcaattcc tcaagaactt tatggtcagt ctatagcaga tagttcgtta
    25441 cgcttagttt ttgagaaaaa aaagacacca aaaaagatca atgctttgcc tagaatcggt
    25501 atcccaaaca aaggtgtatg gacagaaaag ccgttgcgtt ttgttgtgtc gggaaatcct
    25561 tatatctctt tacaaagaaa aaaccaaatc gagaaagatt ggggttggag gaaagaaaat
    25621 gaaaaagaag gatctattaa catatttaga tgaaataatt gaaaaacaag tgacagacta
    25681 tgatgtagct ttagattggg atacaaaaaa tcatacgatc gagatcgttg ttcgcttgtt
    25741 tgcagaaaat aaagcacaat ttgagattga tgatgcagaa gggatcgttt cgcaagaacc
    25801 aattatcgaa tttgaagacg gcgtacttct ttttaatcct cagaaatctg tttttgatga
    25861 acgcaattat ctagcagtaa tcccttatga aggaaaaaaa gggttggcaa aatcagtagc
    25921 tgattcttta gtggaatatc taaatgaagt attagctcaa gggcaaagcg atttgcttga
    25981 ttttttggat gaagacgatg acgaagctgt attcgagctt cactgggata atcaacaact
    26041 agcagaatta gtagaagtaa aacagcaaaa tgaagcagat acttatttac cgtacccaag
    26101 ttattaagga gtgacagata tgaaatggaa tgaagtaaaa gtagaaactg aaagtgaagc
    26161 agtcgaagca gttgctaata ttttgatgga ggcaggggcg agtggtgtcg cgattgaaga
    26221 ttctttagat gttgaacatt ttcagtcgga tcaatatggt gagattttag ataaaaatga
    26281 ttttacatcg ctgaaagaag gcgcactagt catggcttat tttccggaaa cgactttctt
    26341 accggaaatc ttgcctttta tcaaagatcg tatcacacaa ttacctgagt ttggtctagc
    26401 tattggaaaa aataaagtaa cagttagtga agtagaggaa agcaactggg caacagcctg
    26461 gaagaaatac tatcatccgg tgcgtattac gcgaatgctg acggttgcgc caagctggga
    26521 aaactaccag acagaagatc ctctagaaaa aattatcaca ctagatcctg gtatggcatt
    26581 tggcacaggc actcatccca cgacttcctt gacattgaca gcactagaaa cagtcttgcg
    26641 tggtggtgaa acattgctag atgtgggaac tgggtcgggc gtgctaagta ttgcagcaaa
    26701 atatcttggt gcaaaagatg tctatgcctt tgatttagac gaagtagccg ttcgttcggc
    26761 gaaagaaaat atggatatga atgaaatagc caaagatgtc catgtatcag ctaacgatct
    26821 tttgacagga atcgagattg aaagtgatgt aatcgttgcg aacattttag cagatatcat
    26881 cttattgatg atcccagatg cttggcgttt actgaagcaa acaggcacgc tgatcgtttc
    26941 aggaatcatt gaagagaaga agcaaatggt tctggatgcc atgttagaac aagggtttat
    27001 cgtggatcaa attttccagc aaaaagactg gtatgcaatt attttgaaaa aaccagaggt
    27061 ggaatagatg cagcggtatt ttttaaaaga atcatatgaa tcaaaagact attttgtttt
    27121 agaaggggaa aactatcacc acattgtacg ggttatgagg atggaacccg aggatgaagt
    27181 ttttttggca ttcaatgatc aaatagccat tgttgcagaa atcacggaga ttactgaaga
    27241 ggctgtttat ctgaaagaaa ttaaaaaaga agaaaatcaa aaggaactgc caagttcgat
    27301 taccatagcg agtggctatc ctaaagggga aaagctggat tggatcgttc agaaatccac
    27361 tgaattagga gcgcaagctt ttcttggttt cccggcatca gcctctgtcg ttaaatggga
    27421 tgcaaaaaaa cgtatgaaac gacaacaacg gcttgaaaag attgctcaag aggcagcgga
    27481 acaatcccat cggcagatcg tgccatcagt agagttattg gaaacgaaac aagatttatt
    27541 tgagcggcta gatgattatg acgtggtctt ggttgcttat gaggaatcag caaagcaagg
    27601 cgaaaaatca aatgcagcca aaattcttag tgaaacaaaa ccagatagtc gaatcttagc
    27661 tgtctttgga ccagaaggtg gattatcccg cgatgaagtc gattattttg tagaacacaa
    27721 agggatactt tgtggattag gaccaagaat ccttcgtaca gaaacagcac ctctttattt
    27781 attaagtgct gctagctatc aatgggaatt aatgaatcct tctttcagct aacgttcttc
    27841 attaataaaa gagtatcgac agaaagaatt gaaaaaatag tcaacaattt gtataataga
    27901 aacaattatt tatacaatgg atacgaaaaa tcgtttgaaa aagcttttaa aaagcgaaca
    27961 tttccgtaaa aaaacgtata actgcacgga ttttgggaca attatcaaac agatatttgt
    28021 cccgattccc gttttaccga aaaaagaaag ggggatcgat tcctgtgaca aaagaagaaa
    28081 ttttaacggg acccggtgtc ataaagctcg ttagtcaata catgggtccc gaacatgtag
    28141 catttgttga gaaagcatgc gaatacgcga ctgctgcaca tgacggacag tttagaaaat
    28201 caggcgaacc ctacattatt catcctatcc aagtcgcagg tatattagca gatttaaaaa
    28261 tggatcccca tacagtggct acaggcttct tacatgatgt tgttgaagat acagaaatca
    28321 ctttagaaga tctgagagaa gaatttggcg atgacgttgc tatgttagtg gacggcgtaa
    28381 ccaaattagg gaaaataaaa tataaatccc acgaagagca gctagcagaa aaccaccgaa
    28441 agatgctgct agcaatggct caagatttac gagttatcat ggtcaaatta gctgatagac
    28501 tacataacat gcgtacgttg aagcacctgc gagaagataa gcaaagaagg atcgctcagg
    28561 aaactttaga aatctatgcg cctcttgcac atcgtctagg gatcagccgg atcaaatggg
    28621 aattagaaga tacagcactt cgttatctaa atccaaaaca gtattaccgt atcgtccact
    28681 tgatgcagac gaagagagaa gaacgtgaaa aatacgtaag tggtactgtt gaagatattc
    28741 gaatagcgac agaagagctg gggatttttg cagaaatcta tggacggcca aaacacattt
    28801 attcaatcta tcgtaaaatg aaagatcaga aaaagcagtt caacgaaatt tatgacttgc
    28861 tagctattcg agtgatcgta gattcaatca aggattgtta tgctgtatta ggagcaatcc
    28921 acacaaaatg gaaaccaatg cccggcagat ttaaggatta tattgccatg ccaaaagcta
    28981 atatgtacca atctttgcat actaccgtga tcggtccggc aggaaatccg gtagaaattc
    29041 aaatccgaac acaggaaatg catgaaatcg ctgaattcgg ggttgctgca cactgggcct
    29101 ataaagaagg aaaaaatgaa aaagtagaac cagatggtat gacgaaacaa ttaagctggt
    29161 tccatgagat actcgaactt caagacgaaa gctatgatgc ttctgaattt atggaaggcg
    29221 taaaaggaga tatctttagt gataaagtct acgtcttcac accaaaagga gacgttactg
    29281 agttaccaaa aggatccgga ccattggact ttgcatacag tatccataca gatatcggta
    29341 acaaaaccac tggtgcaaaa gtaaatggca aaatggtgca gcttgattac aaattgaaaa
    29401 acggagatat cattgagatc atgacttctc caaattcatt tggcccaagt cgcgactggt
    29461 tgaaattagt tgctactagc aaagcaagaa ataagatcaa acgtttcttc aaagcccaag
    29521 atcgagaaga aaatgtgatc aaaggccacg aatccgtggt caaatgtatt acagatctag
    29581 gatttacgcc taaagatatt ttgacgaaga acaaactgca agaagcactc gatcgtttta
    29641 attatcaaac agaagatgat ctctatgcag ctgtagggta tggagaagtt agccccttga
    29701 cgatggccaa tcgtctgact gaaaaagaac gtaaagaaca aaaaatcgag cagcaaaagc
    29761 aagaagcaga agaaatcatg aatcagccga aaaaagaacc tgacaaaatg aaagtacgtc
    29821 atgaaggtgg cgttgtcatt caaggggtag aaaacttact aattcgtatc agtcgctgct
    29881 gtaatccgat tcctggtgat gatatcgttg gttatatcac taaaggcaga gggatatcca
    29941 ttcatcgtcg agattgtccg aatgttcagc ctgacaaacc aaatgtagca gaacgtttga
    30001 ttgaagtcga atgggaagat acatcgaata cacgaaaaga gtatgatgca gatttggaaa
    30061 tttacggcta taatcgttca ggcttattga atgatgtact tcaaacagtc aatgcgctaa
    30121 cgaaaaatct caacagcgtt gaagcacgga cgaataaaga taaaatggcg acgatccatt
    30181 tgacggttgg tatccagaat ttatcccacc taaagagtat cgtggataaa atcaaagcag
    30241 tacctgatgt ctacagtgta cgccggacga atggatagga ggacagatat gagagcagtt
    30301 gttcaacggg taagtaaagc tgctgtgtca gtcgatcagc aagaaattgg aaaaattggc
    30361 tacggcctac ttgttttatt aggcattcat gaaaccgata cgcaaaaaga tgtggactat
    30421 ctggtaaaaa aaattgcaca aatgcgtatt tttgaagatg aacaaggaaa aatgaatatt
    30481 agtgtagaag atgtgggagg acaaatttta agtatctctc aattcacact gtttgcggat
    30541 acgaaaaaag gaaatcgacc aagctttgtg gaagccgcac gaccagaaac agctattcct
    30601 ttatacgaat cattcaatga aggaatcaga agtcgcggca tcacagtcgc taccggacaa
    30661 ttcggagcag atatgtcggt ttctctcgtc aatgatggac ctgtaacgat cattattgat
    30721 tctaaaaaca aataaagtaa tctggttttc actagtaaaa actatgaaac acgtcgctta
    30781 agttagactg ataaactcta acttaagtgg cttttttgtc tgcaaaaagc gcttcttttg
    30841 tcagcagtcg gaaaaaaagt taagcttaaa ttatacaagt tattttttat aggaggttgc
    30901 tgaagatgaa agaagtacca gtagtagata tcaaaatcac agatttaaaa aaattgtatc
    30961 aaaaaggata caacatgttg gaggagttac gccatgaagc ggacgctccc gtcgtcaaag
    31021 caaagatttt caataaagaa gctatcacga tctacggatc atcagcagca aaagtatttt
    31081 atgatccaag gaatttcaaa cgaaaaggtg ctatgccaaa attagtattg aagacacttt
    31141 ttggtcaagg tggagtacag actttggatg gagctgctca tcaccatcgg aaaaatattt
    31201 ttatggatct aatgacaccg gaaagaatgg aagattatca tcgtatctta gataagaatc
    31261 tgacacaagc acttgaagca cagcatggtc aatttgaact atttgattta agtaagatgg
    31321 ttttctttac atctatttgc gagtgggcag gtatcaattt atcagcgatt tctaaagatg
    31381 aggtcgaaaa attagcagaa tatcaaatct ctatgatcag cggaacgttt acttctccga
    31441 ttgatcacat aaaaggtgtc gagaatcgca aaaaatcaga aaaatgggcg caaggcttga
    31501 ttgaagaagc tcgacaaaat cctgtggctg gaaaagaaaa tgtggcatta tatgcttttg
    31561 ccaatgccac agatttagat ggtcagcttt tacctctgga agtggcagct gttgaattat
    31621 tgaacatcat ccgtccaacg gttgctctaa ctgtgtgggc ggcattgatg ggacatgcac
    31681 ttttcagtag accagatttg tatcagcaat taaaaaatga cttttcgacg ttacaagatc
    31741 cgtttattca agaaatgaga cgctattatc ctttcttccc aatgctacct gcgatttcgc
    31801 tgaaagaagt agaagtagat ggttatcgta taccagaagg cagttgggtt attttagatt
    31861 tatatggaac agaccatgat gaacgaacag ttgaagcgcc agattccttt atgatcaaac
    31921 ggtatgtagg gaaagcaaaa gatatttcct ataaagaaga atatgagatg attgcacaag
    31981 gcgggggaaa ttttcgacaa atgcatcgct gtgcaggaga atggatcact cttcatagtc
    32041 tacgtgtgtt ttccgatcag ctagtaaata agtttgagtt ctctgtacct gaacaagatt
    32101 ggacgattcc attcaatcag tttccgactt atccgaatag ccgagcgcta ctttacaaga
    32161 attagattag cataaactta tcgaaaaaaa ggaagatcaa acagtgctga tcttcctttt
    32221 ttcatattac tataaattga taaggtcaat tgatcaaaat ccacgaaatg cttcatcaat
    32281 ggtttgataa tcttcattgg ataaatgcaa atctaaagct tggacattgt cagatacttg
    32341 ctctggacgc ttagctccag gtataactac agacactcgt ggattcttca tgtaccaagc
    32401 taaaacaagt tgcgcaggag tggcttggta tcttttagca atggggcgaa gtacatcgac
    32461 agcggttaaa atttctctga aacgttctcc ttggaaatct ggatttcttt ttcgaggatc
    32521 tccttcacca aattgttttt cctcaccaag ttcatatttt cctgtcagca aaccggaagc
    32581 aagaggaaaa tagggaacaa aactgatttt gtttttttca agataaggga ataattcttt
    32641 ttcagcctgc cgatggatca aactgtattt atcttctact acatcgacat atccatctgc
    32701 attcgcttct ttcaattgtt ctaacgtgaa gtttgacaca ccgactgctc ggatttttcc
    32761 tgcttctttt aattcgtgca atgtagcaac tgattcattt tttggtgtgc tttcatccgg
    32821 aaagtggata taaaaaatgt cgatgtaatc agtttgcaga cgttttagtg catcttccac
    32881 cgattgtttg agaaactcag gagaattatc aaatgttcgc cctttatttg gtacatgagc
    32941 agcttttgta gcgatgacga cacgtgagcg atcatattct ttttccttca gtacctcgcc
    33001 aatcagttct tcggaacggc cgtttccgta agcataagcg gtatcgatca aattgatccc
    33061 gctattcaaa gctgtacgaa ctacttgttt tcctgtctca tcttccaaac cgtcaaaaag
    33121 attatgtcct ccaacggcat ttgctcctaa tccaagctgt tctgcgtaga cttgtgaatg
    33181 tccgattctt atttctgcca tagctgattc ctcctttttg tctagtctaa aacaaaatac
    33241 caaaaagctg aactaaaacg tttttgtgag gggaaatagt cagatatctc ttgactttga
    33301 gactggtttt tagtaggtta taagtaatat cgaatgtcaa tgaaagagcg agtaagtttt
    33361 tgaaacttta caagagagga tcatcgaggc tgaaaatgat ctaaagtttc attagcctaa
    33421 agacactctg gagatggacg agtgaaaagc cgtcccgcgt acgagcgtta ttcgtaagag
    33481 gaaagagaaa tctttcaaaa ttaggtggta ccgtgtatga atttcattca cccttgttca
    33541 tgatgaacaa gggtgatttt tttatttaca tagctaagaa agggagagaa aactatgagt
    33601 ttccaaagac caaaaggaac aaatgatatt ttgccaggcg aatcagaaaa atggcagttt
    33661 gtcgaagaaa cagcacgttt attattcaga gattatcagt ataatgaaat gcgtacaccg
    33721 atttttgaac actatgaagt gatatcccga agtgtagggg atacaacgga tatcgtttca
    33781 aaagagatgt atgattttta tgacaaaggg gaacgccatg tgacgcttcg accagaaggt
    33841 acagcaccaa tcgttcgttc ttttgttgag cataaattgt tcggcccaga gtatgcgaaa
    33901 ccgttcaaaa ctttttatat gggaccaatg tttcgctacg aacgccctca aaaaggacgg
    33961 ttacgtcaat tccatcaaat cggagtagaa gcatttggat cagaaaaccc tgcaactgat
    34021 gtggagagca tggtgatggc acttgatttc ttcaaacagt tggggatcac gcaaatccgg
    34081 ctagtcatca attcattagg tgataaagaa acgcgtcaag cctatcgtca agcattgatc
    34141 gactatcttt taccttttga atcagagctg agtgaagact ctaaacgccg ccttcatgaa
    34201 aatccattac gcgtattgga cagtaaagac aaacgagatc aaaaatttgt ggcagaagct
    34261 ccatctatct tagattatct aagcgagcct gctcaaaaac attttgatac cgttgtttcc
    34321 atgctggatg cattggctgt tccttatgaa atcgacagta atatggttcg cggactagat
    34381 tattacacac atacgatttt tgaaatcatg agtgacgcac caaaaatggg ggcacaagca
    34441 acgatttgcg caggcggacg ttatgacaat ctagtggaag aactaggtgg tccttcaaca
    34501 cctggcttcg gatttgcaat gggtatcgag cgattactct tgactatgga ggcagaggaa
    34561 gtagttatcc ctgcatttaa tgagttggat gcttacgtgg tcgcactcgg agacgaaaca
    34621 aatatcgaag cattgaaagt ggtccaagcg attcgaaact ttggtttttc agctgaccgc
    34681 gacttcatga accgtaaagc aaaagcgcaa tttaagacag cagataaagc gaatgccaaa
    34741 ttagtattgg tgatcggtga agatgagctt gctagtcaaa cgattaatgt caaagcaatg
    34801 gcaagccgaa aagaaaaaag ttttccatta gaagagatat atgaacattt tgatgaagtc
    34861 tatgatgaaa tgactttatt cgagtaggag gaaaaaaata aatggcacaa agaacgatct
    34921 attgcggatt agtttccgaa caacaattag aacaaacagt tacattaaaa ggatgggtac
    34981 aaaaaagacg agaccttggg ggagtaatct ttatcgacct acgtgatcgt gaaggaatcg
    35041 ttcaaatcgt ctttaatcct gaaatcaaca aagaagcatg ggaaatcgcg gataaatgtc
    35101 gcagtgaata cgtgatcgaa gtgactgggg aagttagaag acgcggagaa aatgcagtaa
    35161 atcctaaaat ggccactggt gaattcgaag tgattgcaag tgaaatcgtg attttgaatc
    35221 aagcaaaaac cccgccattt caaattgaag atgatcaagc aatcagtgac gaaatacgtt
    35281 tgaaatatcg ttatttggat ctgcgtcgtc caaaaatgac taaaaacatc aaattaagaa
    35341 atgcgacaac aaaagcgatt cgtcattatt tagatgataa tgactttctc gatatcgaaa
    35401 caccttacct agggaaatct acaccagaag gcgcaagaga ctacctagtt ccttcacgtg
    35461 tccatgctgg acatttctat gcgttgccgc aatctcctca attgtttaaa caattgctga
    35521 tgaatgctgg attcgaccgt tattatcaaa tcgttcgttg tttccgcgat gaagatttac
    35581 gtggagaccg tcagcctgaa tttacgcaag tagatattga aacaacgttc ttaacagcag
    35641 aagaaatcca aacttacaca gagggattga ttgcgaaggt aatgaaagaa gttcgcggta
    35701 tcgaagtcac attgccattt cctcgtatga cctatgatga agcaatggct cgctacggca
    35761 gcgacaaacc ggatacacgt tttgatatgg aattgatcga tctaagcgat acagtcaaag
    35821 aagtagaatt caaagtcttc caaatggctc ttgaaaacgg cggtgtcgtg aaagcattga
    35881 atgctaaagg cgctgcagat cgctactcac gaaaagacat ggatcagtta ggtcaatatg
    35941 taggacaatt cggagctaag gggcttgcat ggctgaaagt agaagaagac ggattgaaag
    36001 gtccaattgc taaattcatg ggagaagcaa cagaagcaat cattaaagca acagatgcaa
    36061 aaccaggtga cttgctgatg tttggcgcag ataaaagtga aatcgtcgct gctgcattag
    36121 gtgctatccg gacacgttta ggaaaagaat taggactaat tgatgaatcg aaattcaatt
    36181 tcttatgggt aactgattgg ccacaatttg aattttcaga agaagaaggt cgatatgtct
    36241 ctgctcacca tccattcacg atgccaaaag aagaagatat cccgttattg gctgaagacc
    36301 ctgcaaaagt ctatgcagaa gcttatgata tcgtcttgaa cggttatgaa ttaggcggag
    36361 gttctatccg tattcatact cgtgagcttc aagaaaaagt actagaaact ctaggcttta
    36421 caaaagaaca aatggaagaa caatttggtt tcttgttaac agctttagat tacggattcc
    36481 ctccacatgg cggtattgca ttaggattgg accgttttgc aatgttatta gccggagaag
    36541 ataatattcg tgaagtcatt gcctttccta aaaacggaaa agcagcagat gtgatgagtg
    36601 atgcaccaag tatggtttcg cctttacaat tgttcgaatt gaatattgat gtaacgaata
    36661 tagaagaata gccgataaaa aggtaaaaag gatttgtcga atacgcttct tttgacaacc
    36721 ttgatttaat tgaaaaacac tttataatga agccataaca tataaagcgg ggcttttcca
    36781 tgaaaaaaac attcgaaaat tgggcttatc atgattacac gaccaagctt tcggtatcta
    36841 tcgtttatgc gttgatcgca tcagtcgcat tgaatttctt ttatcagcca ggacatattt
    36901 attcaagtgg tattactggt ctagctcaga ttttgacgac tttgtcaaaa tcactgatag
    36961 gattcgaagt accggtatca acaactttgt atttactgaa tatacctttg tttgtattgg
    37021 cgtggttaaa aatcgggaag aagtttacaa ttttcacttt tatcacagtc actttgacct
    37081 ccctttgtat ccatatttta ccggaaacag tcctatctac cgatccaatc atctgtgcgt
    37141 tgtttggggg ggctgtgatg ggactaggta ttgggctagc gttgagaaac ggcctatcat
    37201 cgggcggtct agatatcgtg agtatcacga ttcgtaaaaa aacaggaaag tcgattggtt
    37261 ctatttcgat ctacttcaat gcattgatcg tttttgttgc cggaattcta tttggctggc
    37321 aatatatgtt ttacagtacg ttgtctattt tcgtcagtgg aaaagtaact gatgtcgttt
    37381 atactaaaca aaagaagatg caggtaatga tcgtaacgaa acatccagaa aaagtagtaa
    37441 atgaaatcca aaactgtatg cgtagaggga tcacgatcat caatgaagca gaaggtgctt
    37501 ataagcatga taagcagaca gtattgatga cgatcgttac acgatacgaa ctgcctctac
    37561 ttagagaagc aatgaaagct tctgatccac aagcttttgt aagtattgca gaaaacgtcc
    37621 agatacttgg tcgtttctat gaggatggca tttagatcga gtttgacaaa aattcatgaa
    37681 atactgctgt agacagacgt tcgtttgtct gcagtttttt tctaccatag tagaatcagc
    37741 aaagaaaaac tgctttttta tttgaaattt tcttaaagtt gaaaaaaagc atgtttattt
    37801 ttttaaattt atgggatgat agaaaaaaat agtagagaaa ggacacgatt ctacttgcta
    37861 ttttcactta atacccgtat attacgaatg aaaaaagttt atgaaaagtt ggaatattga
    37921 atggaaaaac aaaaaaggtt aaaaaacagc cgctacatca ctgggtttga tgggctccgt
    37981 tcattagctg tggtgggtgt tatcttctat caccttctgc caactagtat gaaaggcggc
    38041 tatttgggcg ttccgatttt tttcgtcgta tctggttatt tgatcacgga tctgctgcgg
    38101 caggaatggg aacaaaatgg aaaaatcaat atttggcaat tttatgtcag aagaatgaag
    38161 agactctatc cggcacttgt ttttatgctt gtgacagctt cggcttatat cactttgttt
    38221 cagcgagggc tgttaaacaa tcttcgagga gttgtggtca gcagcttact gtatgtcaac
    38281 aactggtggc aaatcaataa cggtttatct tatttcgatc gatttgctaa tgagtcaccg
    38341 tttacgcata tctggtcttt agcagtagaa ggtcaaaact atttggtttg gccgatcttg
    38401 tttgtattgt tgatggtatt tgttcgtaaa agaaaatgga tcttttatac tgtgttaggt
    38461 acatccttcc tctccgccat cttgatgatg atcttgtata caccaggagg cgatccaacc
    38521 cgtgtttatt acggtaccga cactcgtctg ttttctatat ggttgggaag tgcgcttgcg
    38581 tttgtttggc caagtacgcg tttaaagaaa aacatcccga aacaagcgaa aaatattctg
    38641 aatatcgctg gaggaatctc gcttttggca ctgattcttt gcttcttctt tttagatgat
    38701 cattacaatt tcatctatta cggaggaatg ctcctagtga gtctgttatg cgtagtgcta
    38761 gttgcagtga cagcacaccc cggagcaagt ttgaatcgat ggctgacaaa tccagtattt
    38821 acttggattg gcaaacgaag ctatggcatt tatctgtatc agtttccagt tatgattttt
    38881 tatgaagcaa aggtgactga tattgccgat catgttttgt tgcatacttt agtggaaatc
    38941 acattgattc taatcatcag tgaactttct tatcgatatg tggagcgtcc gctagcacgt
    39001 tttgattatg gtcgtacatt ccaagtgatg aaaggatggt tcagcaaacc gatcatcagt
    39061 aagagcaaac catgggttgt tccaggcaca ttacttgtag caattgcact cgttggtttc
    39121 gttacggcac cgaaaaacgc tgtaacagcc gatcaaaaac aactgcaggc aaaaatcgct
    39181 gaaagtaaaa aattagcaga agaaaccaga aacaattcag aaaacaccac tagttcggtt
    39241 gaccaagcga tccttgataa gtacgagttg tccaaggcac aagggaataa ggcacaaaaa
    39301 ttagaactaa cggcatttgg tgattcagtt atgttggatg ctgcaacgga tctacaagaa
    39361 ctttatccaa aagtagtggt agatggagat gtcggacgac aattatatgc cagtgtaccg
    39421 tacattgaaa aattaaagga acaagatttg ttgaaagaca ctgttctgat tggactagga
    39481 acgaatggct cattctcgga agctcagttt gatagtgtga tggatgcaat cggtgatcga
    39541 aaagtatact gggtcaatgt ccgagtaccg acgaagcgct ggcaaaatga cgtgaatgcg
    39601 atgcttgaac agatgacgaa aaaatacgac aatatgacgc tgattgattg gtacagctac
    39661 agtaatgacc atagtgactg gttttatgat gatcaagtcc atccaaatcc tgaagggatg
    39721 acacattaca ttcacttagt cagcgaagcc ttgctaggag gaagttcaag tacgaacagc
    39781 agtgagacaa acgtgacaga atgatagcta caaaaaactt caggcaaaaa catttgtttt
    39841 tgcctgaagt ttttttatat ttaccgtgtt tttaacattc atatagattc aggaagcaaa
    39901 acggttgagt gtgtctgtaa ataacgttct ttttgttttc gggtgagttt tttgaatgca
    39961 gcttctgctt ttgttgcttc gcttcttgaa gaaaatactt ctgcatggat catcttaaca
    40021 ggtcttcttt tttgaagacg agtatattta gcaccagtac cattgttatg ttcctctaat
    40081 cgtctagaaa gatcggttgt atatccacca taaaatgtct gatctttaca gaataatacg
    40141 taaaaataat gttcatttgc catagagcat ctcacgaatc tctgcagtgt attctccatc
    40201 catattgtaa gtgatcaatg gtggagcgac tttgaatccg tctaatttgc cttctttgat
    40261 tccttcaact aacaaaatat ttgcctcttt tccaggtttt gggtaaacaa attggatcct
    40321 tttaggtgca attcgatagt cttccatgac ctgaatgatt tctaagaacc gatcaggacg
    40381 atggaccata gtaaagcgtc catttgtttt tagtagctta cttgaaatat cgatcacttc
    40441 ttctaacgtc gtatgaatct catgacgggc gattgccagg tgtttattag ggttcgtttt
    40501 atttgttggc agccctttga aataaggtgg attacagacg actaagtcac aagaattatg
    40561 tcgaacgaca gataagctgt ctttcagatc gatggtgtgg acagtcattt gttcatttaa
    40621 atggttaagc gcgatactac gtcttgccat gtctgctaga cgaggttgaa gttcgatagc
    40681 gtcgatagcc gcctgggttc gtttacttaa aaaaagtcca actgcgccat ttcctgcaca
    40741 aaggtcaaca atcttccctt ttttaggtac acgggaaaag tgagcaagca gcacagcatc
    40801 taaagaaaaa gaaaacactt tggaactttg gataatcttg atatcttctg cgtataattg
    40861 gtcgattcgt tcgtcttcgt gaagcatgga ttccctccaa aatgaaaaaa atagtcgtct
    40921 gtattatact atttttatta aaaagaacca aggtggcttg tcaaacctct attttttatg
    40981 catactatga ccataagaag ggaaaggaag agactatgtt ttatcgattt atgcgcgggg
    41041 tagttcgagt actattagcc ttagttaatg gaaatgcccg ctatgagaat aaagaagttt
    41101 taccgcaaga tgagaattac atactagttg ctccccatcg tacttggtgg gacccattat
    41161 atctggcggt agctgcagca cccaaggaat tcagctttat ggcaaaagaa gaactgttca
    41221 aaaatccagt tctgcgattt atattaactc gatccaatgc ttttccagtt aaaagagaaa
    41281 atccaggacc aagcgtcatt aaaacacctg taaagatttt gaaatcgaca aatttgagtt
    41341 tgatcatgtt tcccagtggg acaagacatg ctacagaact taaaggcgga atggcattga
    41401 tttccagaat gtctaaagcg aaaatcgtgc cagctgtata tcaaggtccg ttgacacttg
    41461 gggatctgtt caagcgtcag agagtgacag tgcgtttcgg cgaaccaatc gacttatcag
    41521 atatcaagaa aacggataaa gaaggtatgg cggagatcga acgacgaaca caagcagctt
    41581 ttgacaaatt ggacaaagaa gtgaaccctg atttcaaata tgaagtcaaa aaaaaataaa
    41641 atgataagtt tatgcgtggg cggtcttttg aatctactgt cctgttcagt tcagactttt
    41701 tcaatgaaaa cgagggagag acgtcaagtc tgccctcttt ttgcgttcca atatgtaaaa
    41761 tagaagacag gtgtcatgta atggatcaaa gtgttataat aattagaagg acattttttg
    41821 aggtgattga atgcgttttt tacatacagc cgattggcat atcggaaaaa aactacaagg
    41881 ttatgacttg attgaagacc agcgtcatgt actatcttct atactagata tcgcgaaaga
    41941 agaaaaagta gaggcaatcg tgattgccgg agacttatat gataggagtg tcccagctgt
    42001 tgatgccgta gaattataca atgaattgat ggtcgattgg aatttaaagg aaaagatccc
    42061 gatttttgct atatcaggaa atcacgatag cagtacgaga ctttcagcgg gtacgccatg
    42121 gttcagttat tcgaactatt ttttgcgaac acagctagca gaagcatttg aaccagtcga
    42181 gtttaaaaac gtacaatttt tctttcttcc ttactttgaa ccaattgcag cgagattata
    42241 ttttgaagat gacaatatcc gaacgatcaa gcaagcggta gccaaggtga tagataaaat
    42301 gcaggagcta tttgattcag aaaaaaaaca agtattgatt agccattttt ttgtaatggg
    42361 aagtgtaaaa tctgattcgg aaacgaaaat cgaagtaggc gggttggatt cgatccctgg
    42421 tgaactactt acaccgtttg attatgtagc actaggacat ttgcataatc aagctgcttt
    42481 gagacaggat aatgctcgct atagcggttc cccattgaaa ttctctttat ctgagatcaa
    42541 ccaaaaaaaa ggcgtatgga tcgttgacct gacaacaggc ctggagttga attttaggga
    42601 gatcgaaccc ctgcatgata ttgaagaaat aacagcgagt ttcaaagaat tgctggatcc
    42661 tgccttttat cagtccgtca atcgggagaa ttacatctat atctatctca ctgatagaac
    42721 gatcatcccg aatatgatga accagcttcg gcaaatatat cctcgtattt tggggattga
    42781 acgggcaaac ggtcgagcag aacaaacaaa gcgcaaacga aaagtactag aaatgaaaga
    42841 tcctcaaaaa ttagcagaag cattttttag tgaaatgacg gaagagcagc taactgcaaa
    42901 ccaagaaaaa tggctcggag aaacgttgca agaaatcatg gaaatggaat gatcaagaat
    42961 gaaaccgaaa aaattacaac ttaaaaattt tggtcctttt atcaatgaga ccgtagattt
    43021 ttcacgtttg acagaagcac cgcttttttt gatcagcggg aagacaggag ctggcaaaac
    43081 gacgattttt gatgggatca ccttcgcact ttttggtgaa acttccggaa gacttcgctc
    43141 tggaaaagag atgcgttctt tgtttgctac accagacgaa gaaacaagcg tgactttttc
    43201 ttttgaacat cagcagatga gctatgaaat cgaacgaaaa ccagaacaag ttttagcaaa
    43261 acgaaaagga aacggaacaa aaaaacaggc agccaaagta acgttgacca tttttgatgg
    43321 gaaaggaaaa gaaataaaac aaatcacaaa acgtacagaa gcagatcagt tgatcagaga
    43381 actgatgaat ctcgatgcga aacagttttc tcaaatcgtt ttactacccc aaggagaatt
    43441 ccgtaatttt ttggtttcat caagcagtga taaagagact gttttacgtc atttattcgg
    43501 tactcaattt ttccaacagt tcaatgagcg attaaaagaa aaagcaaaac aacagcaaca
    43561 aaaattggat catttagagc aagagctgca tcttctgcaa aaaaggtttg ttttagtcaa
    43621 agaagaggaa ccagcagcag ggtttgaagc cgttttagcc caatggacaa accatcaggc
    43681 aatactcgaa aaacaaatca cggaagaaaa agaagctcta acggaacttc aggaaaaaca
    43741 aaagcaacta gaaaattcct actatcgttt tgaaagcttg aaaaaaagtt atgaagaaaa
    43801 aacgatatta ataaacaagc aaaaggaatt agctgctcaa aaagacgaaa tagaggaaaa
    43861 gaaacgatgg attgggtatt atgaatttac gcaacgatta gtcgaaccaa tccagcgcct
    43921 acaagaaata aaagttgaaa aaatagaact gtcacaaaaa agtagtgagc aaacaaagaa
    43981 actagcgatg actacgcaga attatgaaga gtggcaaaaa aatgaagcaa accgcgtacg
    44041 ccgacaaaca aaaatcaaag aagctgctca aaaactgcaa aacaaacaga tgatggtgcc
    44101 aattgtcgaa tccttacgta gtaaaaaaca agacgcagaa caattaacaa tttcattgga
    44161 taaagaagaa ttgaatgccg agaagctgta tcaaaatctc gctatctatc aggaaagaac
    44221 tcgaattctt caagaaaatt tactggatca ggaaaaactg caagaagatc gtcttgcttt
    44281 tggacagtta aaatatctag atgaaaagca gcaagaaata gaaaaacaaa aagcaaaaga
    44341 cactgggtcg ctcgcacaga taagagaaga actcaaaaaa gcaactgaag aaataacaaa
    44401 aaatagtcag ctattgaaag aacagacaga acaagcagaa cagctgaaaa gccaatgggc
    44461 aaagcagcaa attgctcgtc ttcagctttt tttgcgtcca ggggagccat gtcttgtttg
    44521 tggatcaacg gaacacccca tacaacaatt aaaacacgaa cagccaaata aggaagatat
    44581 cttaaaagta gaacaacaac tggcagaagc tgagtctgct gtagaaagaa ctcagcaaat
    44641 cattgccaaa agtgaagcac atgaaaataa tcttcaacaa caattatcag atttagaaca
    44701 aaaaatcatg gcaacgaata tacaattggc tgaacaagaa gaacacttgc aacaggcttt
    44761 gaaaaaggca tttcctgaga taacagaaca aaaaacaaaa acaactcttg tagtaatcga
    44821 acaatcttta gatagacgaa aaagcaagat ggccaatgca agaatagagc tggaagaaat
    44881 aagacaacaa gcagaaaagc tcgaacaaca attagctgtc tacaaacagc agcagacaga
    44941 aaaaaataat cagctccagc aactgcaagg agaaatcaaa agtttagaac aacaattgca
    45001 agatacggat agtaactgtt tagaagagga gatacgacga cttgaaaaac agttagaaga
    45061 agactcgaat caactagctg ttgagcagaa agcaggagtg caattgttgc aagatcttca
    45121 tacgcttcgg acacagcttg atcttcaaca tgaacaaata gaaaaaatag atgaaaaaat
    45181 agaaaaggaa caagaaagaa tagctaaaaa gttagaaagc cagtcgtatt tttcaacaat
    45241 tgaagaaatc tgtgcatttg cacaacagca agcacaatat aaagaaaacc aacgtacagt
    45301 tacttcttat catgaagagc aattagtgac agctgaccgg ctgcgtcaat tagaaaatat
    45361 ccagctaact gagacattcc cagatacaga agagatcagc caacagttag aagaaataag
    45421 acgtatgatc gaaataaagc aagaaaagat atacggttta caagaacaaa aaattagtaa
    45481 tcaaaaaatc tacgatgaat gtctttcgat ttatcacaca agccagacga aattagatga
    45541 gcttacgcaa ttacagcagc tttcgcaaac attcaacgga gaaaatccta agaagactag
    45601 tttggaacgg tatgtgcttc aggtttatct tcaagaagtg ttacaagtag caaatgatca
    45661 tctccaacgt ttaacgaaaa atcgttatca atttgagcta gcggatacca ttggcagtta
    45721 tcgagggaaa accggattag aaatcaatat ctatgatgat caggcaggta tgtctcgaag
    45781 tgcgcatacc ctgtctggcg gtgagagttt tattgcagca ctttctttag cattagctct
    45841 tgccgatgtg atccagacac aagctggtgg tgtagcaatc gatgcattgt ttattgatga
    45901 aggatttggg tcattagatg aagaagcttt ggaaatggca atggaagctt tagaaacgat
    45961 cgaaagtgaa ggaagaatga tcggcatcat tagtcatgta agagaattga aagaacgagt
    46021 attacaacaa ataagaattg atacagaagg tagcggacaa agcaaaatac gctatcaact
    46081 tggataaaaa gggagattgg taactatgaa atggagtatt ccagaaaaag tcattgcacg
    46141 tggacgtcaa tacttgcaag aaggccgagt gttatcggtt gtccctgacc aagaagaaaa
    46201 cgtgtggcat gcagaagtct tgggcagtga attgtatatg gttgatttgg atggtacagc
    46261 aaaagagata gattactgcc aatgtcccta ttgggaggaa cataaatatt gtaagcatac
    46321 agttgctgtg gaactttatt tacggtcgaa aaatctatct cgtatcatga aaaaagacca
    46381 gccaatgcta gaaccggtaa aagcaacaag tgagggagaa atcattgcaa aaggtttttc
    46441 tcgtctaaaa acacaaaaca atcctcaagc ggtgcaacct ttgattattg aataccaagt
    46501 agaaacgatt gagactaatc agtatcaccc agagctttct attttggcac tttacttgcg
    46561 tatcggttca ttggcatcac cgaaaaaaac atatatcgta aaaaacatct atgatttttt
    46621 acaagcttac acagaaaaag aaacttacac tgttaataaa cagtaccagt ttcgtctaga
    46681 taaacgggcg tttcaaaaag aagatcaaaa aatactcacg catcttgcag gaagtgctca
    46741 gacgcatcaa ttattaggta cgaacggctt acaagtcaaa ggaaaactag ataaaagata
    46801 tctgcttctc ccagtagaac aagcccgctt cctgttagag cagatgaatc agactacacg
    46861 tttgcatctg cagataaatg aacaaaacga tcaaggaata caattttccg atcaaaacaa
    46921 acctttatca ttcaaagtgg aaaaatttgg agaagattat ttgctgcaag cattgaatga
    46981 ttttgatttt ttctttatgc attatcagtg gggagttatc caagggacat tttattctct
    47041 gacgaaatat caacaaacgg tttatcaaac ttggaaacaa ttgattcgcc gattggagaa
    47101 accagaagta gtgttcaaga aaaaagaatt accgtctttg tttaaagaga ttatccctct
    47161 gctgtcagag atcggcgacg tagctattgc ccctgcagta gaagaggaag tagcagatat
    47221 ccccgtcgaa tttattttct ttttccgaaa agcaaaagga aaaatccaag ctcgagtcga
    47281 tttcgtctat ggtgaagtca tctattcgac tgacccgaag catgaggtct cgacagaaca
    47341 ggcttttcag gtgttacgtg acacagtaga agagaaacga gtacttgatc tattcagaat
    47401 gtatcgctac cagaaaaatg aaactggtta tgaacgtcca ttgccaagcg gggaagaact
    47461 atatgccttt ttccgcacag aactatctgt cttccgtcag tttggtgaag tcagacttgg
    47521 aagaaaattg cgagagttgt atttagatgc gcataaacat caaccgaaaa tcgaagtaac
    47581 cgagaccggt tcatggctcg atattcgatt cgatgtgaca gggatagagg agcaagaaat
    47641 cgatcatgtc ctgcaaagct tgctgagaaa tgatgctttt tatacattag aaaatggtca
    47701 ggttctttct tttgattcag aagaatttca acaaacaagc caagtgttgc aacaattacg
    47761 ggaatcgata cgtacagaag aaggaacgat ccatgtacct aaaaatcaag gattgatcat
    47821 tcaaaatcaa ttggaaaaaa gcaatgctac ttttagtgaa tcgttccaaa cgatggtaca
    47881 agatctcatt catccagaaa gataccaggc acagcttccc aaaggattga atgcaacgat
    47941 gagagattat caaaagcaag gttttcgatg gttgaagatg ttaggtcatt atcagtttgg
    48001 cggtatttta gccgatgaaa tgggacttgg gaaaacacta cagacaattg cctttttact
    48061 ttcggaaaaa gaagaacgga aaagcttctc tgctttgatc gtagcgcctg cgagcttaat
    48121 ttacaactgg caggcagagg tgagaaaatt cgccccttct ttaagtatcc aagtaatcaa
    48181 tgggaataaa aaagaacgtg aagaattact ggctaaagac acagatatcc gagtgacttc
    48241 ttatgccagc ttaagacagg atctagctaa ttatcagtcg caaaaaattg attatttgat
    48301 tcttgatgag gcacaaatgg tcaaaaatag cagtacgaaa actgcacaag cattgagaga
    48361 attagctgta cctcagcggt ttgcactgag tgggacacca atcgagaata atttagaaga
    48421 attgtggtca ttgtttgcaa cgattatgcc aggatttttc ccgactaaaa caaaattcag
    48481 ggaacttgcc gcagaagaaa ttgcgcaaat gattcgaccg tttattttac ggcgagataa
    48541 acaaactgtg ttgaaagact tgccggaaaa aacagagatg aatctatatt ctgcacttac
    48601 agaagaacaa aaaactgttt atttagctta cttgcgtcaa atgcgtgaag aaatcacatc
    48661 aatggattct gaagcattca agaaaaaccg gatcgggata cttgctggtc tgacaagact
    48721 gcgacaaatt tgctgcgatc ctcgtctgtt tattgaagat taccaaggcg gttccggcaa
    48781 gctcgaacaa gtaaaagatc tactggtagc tgccaaagag aacaaacgaa gagttttgct
    48841 gttttctcaa tttacaggga tgctgactat cttgcaagaa gagttagccg agctagggat
    48901 ctcgacattt tatttaagag gcagtacgaa accacaagat cggttaagta tggtagacgc
    48961 atttaatgct ggcgaaaaag atgtttttct gatttcctta aaggctgggg gaactggatt
    49021 gaatctcacg ggagcagata cggtcatctt gtatgacctt tggtggaatc ctgccattga
    49081 agaacaagct gctggacgtg cacatcggat cggacaaaaa aatgtggtag aagtgtggcg
    49141 tatgatctct gaaggaacga ttgaggaacg catggattca ctccagcaag aaaaacgcga
    49201 actgttccaa aaagtgatcc aaggtaatga agaacagttg acgaaactga ctgaagaaga
    49261 catccgcatg attttaagtg taggagaaat cgaggagtaa gagactcgaa aaagcgttca
    49321 gtaatgggta agaaagcctt tctaaagaag cgattgttta tttgatttaa aaaatagcaa
    49381 ggaggctggg attccatttt atcccagctt ccttgctatc atttttttat ttctattttt
    49441 aaaggttata caaagattgt cttcattgtc tctaacagct gatcaaaaga agaaatctcg
    49501 atatcgcaaa ttggagtctg ttcaattttg cgttctcgat ggttgaacca aagcgcttgc
    49561 cagttagcac tttttgcacc tagaacatca ttgtcgtaat tatcgccaac gtataaggtc
    49621 tcttctggaa gcatatgaaa ttctttctca gccagctgaa aaatctcttt ttctggtttt
    49681 tggaaacctg ttgcttgaga aatgagcata ttgcgggaag gaatccaatt gttcaattga
    49741 agttgcttca gtttttttgt ctgatggtca gtcggcccat ttgtgataat tcctaatggg
    49801 acattctttt ccttcaagaa atcgagggtt tttttgacct cctcatgcaa gcagatatta
    49861 tcaagttctt cctcgtaaat tttttgaaat aagaggccat cttcttccgt aatatgaggg
    49921 tagtctaaat cttttagtga ttggctgatc cgatgtgctc gcatatattc tagtgtccaa
    49981 tctcctgcca ttacttttgg gaagttttca tcactatgat ggcggaaccg gatatatagt
    50041 ggatgcatat ctgcatctga aacaagcgga acgactcttt tgaccgcgtt tcgaaatggc
    50101 tgctgctgat catacatcgt atcatcaaca tcaaacacaa ctgatttaat caaaatttta
    50161 cacgcctttc ggttttcatt gattttttca caaatccttg actattgtaa cagaaaaaat
    50221 tgtctaaaaa agctgataaa taaaggtttt tcttactata tttctatttc cttgacagaa
    50281 taaggttttt atatttcagt ttttttcact aaaaatcgtg aaacacgtca ataagtttga
    50341 tgaacaaatt aatcattatg gtagaagtcg attgatttat tttaatgcaa tctctgtgga
    50401 attttcgaaa gatttatttt tttaatacag tctcaatata actattcaaa cataaaataa
    50461 cgttatttga tttatttgtt tatagtaaat tttatttagt aatcattgct ttaaaatact
    50521 acgcttagag agtgtaaaat attttgtgta aatgaaaaaa tccatacaaa aaaggaag
//
LOCUS       DAHHZJ010000003        49456 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.003, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000003 DAHHZJ010000000
VERSION     DAHHZJ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.003"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(266..1993)
                     /locus_tag="NBL02_000097"
     CDS             complement(266..1993)
                     /locus_tag="NBL02_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HCD2570814.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(1986..3179)
                     /locus_tag="NBL02_000098"
     CDS             complement(1986..3179)
                     /locus_tag="NBL02_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HCD2570815.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3366..4010)
                     /locus_tag="NBL02_000099"
     CDS             complement(3366..4010)
                     /locus_tag="NBL02_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCD2570816.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4104..4238)
                     /locus_tag="NBL02_000100"
     CDS             complement(4104..4238)
                     /locus_tag="NBL02_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HCD2570817.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4240..6372)
                     /gene="feoB"
                     /locus_tag="NBL02_000101"
     CDS             complement(4240..6372)
                     /gene="feoB"
                     /locus_tag="NBL02_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HCD2570818.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6369..6842)
                     /locus_tag="NBL02_000102"
     CDS             complement(6369..6842)
                     /locus_tag="NBL02_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HCD2570819.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7243..7467
                     /gene="nrdH"
                     /locus_tag="NBL02_000103"
     CDS             7243..7467
                     /gene="nrdH"
                     /locus_tag="NBL02_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCD2570820.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7626..9785
                     /gene="nrdE"
                     /locus_tag="NBL02_000104"
     CDS             7626..9785
                     /gene="nrdE"
                     /locus_tag="NBL02_000104"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCD2570821.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            9835..10800
                     /gene="nrdF"
                     /locus_tag="NBL02_000105"
     CDS             9835..10800
                     /gene="nrdF"
                     /locus_tag="NBL02_000105"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCD2570822.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(10889..12028)
                     /locus_tag="NBL02_000106"
     CDS             complement(10889..12028)
                     /locus_tag="NBL02_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCD2570823.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12337..13050)
                     /locus_tag="NBL02_000107"
     CDS             complement(12337..13050)
                     /locus_tag="NBL02_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HCD2570824.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13304..14005)
                     /gene="nagB"
                     /locus_tag="NBL02_000108"
     CDS             complement(13304..14005)
                     /gene="nagB"
                     /locus_tag="NBL02_000108"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCD2570825.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14239..15771
                     /locus_tag="NBL02_000109"
     CDS             14239..15771
                     /locus_tag="NBL02_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCD2570826.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(15962..17164)
                     /locus_tag="NBL02_000110"
     CDS             complement(15962..17164)
                     /locus_tag="NBL02_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD2570827.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17246..17935)
                     /locus_tag="NBL02_000111"
     CDS             complement(17246..17935)
                     /locus_tag="NBL02_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCD2570828.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18041..18409)
                     /gene="rplL"
                     /locus_tag="NBL02_000112"
     CDS             complement(18041..18409)
                     /gene="rplL"
                     /locus_tag="NBL02_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCD2570829.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(18462..18965)
                     /locus_tag="NBL02_000113"
     CDS             complement(18462..18965)
                     /locus_tag="NBL02_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCD2570830.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19169..19296)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(19375..20064)
                     /gene="rplA"
                     /locus_tag="NBL02_000114"
     CDS             complement(19375..20064)
                     /gene="rplA"
                     /locus_tag="NBL02_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCD2570831.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20168..20590)
                     /gene="rplK"
                     /locus_tag="NBL02_000115"
     CDS             complement(20168..20590)
                     /gene="rplK"
                     /locus_tag="NBL02_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCD2570832.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            20933..21601
                     /gene="sdaAB"
                     /locus_tag="NBL02_000116"
     CDS             20933..21601
                     /gene="sdaAB"
                     /locus_tag="NBL02_000116"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HCD2570833.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            21614..22486
                     /gene="sdaAA"
                     /locus_tag="NBL02_000117"
     CDS             21614..22486
                     /gene="sdaAA"
                     /locus_tag="NBL02_000117"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HCD2570834.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(22577..23596)
                     /locus_tag="NBL02_000118"
     CDS             complement(22577..23596)
                     /locus_tag="NBL02_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HCD2570835.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(23769..24674)
                     /locus_tag="NBL02_000119"
     CDS             complement(23769..24674)
                     /locus_tag="NBL02_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCD2570836.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(24733..25278)
                     /gene="nusG"
                     /locus_tag="NBL02_000120"
     CDS             complement(24733..25278)
                     /gene="nusG"
                     /locus_tag="NBL02_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCD2570837.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25379..25549)
                     /gene="secE"
                     /locus_tag="NBL02_000121"
     CDS             complement(25379..25549)
                     /gene="secE"
                     /locus_tag="NBL02_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCD2570838.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(25573..25725)
                     /gene="rpmG"
                     /locus_tag="NBL02_000122"
     CDS             complement(25573..25725)
                     /gene="rpmG"
                     /locus_tag="NBL02_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCD2570839.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            25887..26531
                     /locus_tag="NBL02_000123"
     CDS             25887..26531
                     /locus_tag="NBL02_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCD2570840.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(26587..27090)
                     /locus_tag="NBL02_000124"
     CDS             complement(26587..27090)
                     /locus_tag="NBL02_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HCD2570841.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27103..27149)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            27687..28652
                     /locus_tag="NBL02_000125"
     CDS             27687..28652
                     /locus_tag="NBL02_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HCD2570842.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(28751..29923)
                     /locus_tag="NBL02_000126"
     CDS             complement(28751..29923)
                     /locus_tag="NBL02_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2570843.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(29910..31055)
                     /locus_tag="NBL02_000127"
     CDS             complement(29910..31055)
                     /locus_tag="NBL02_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2570844.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(31069..31993)
                     /locus_tag="NBL02_000128"
                     /pseudo
     CDS             complement(31069..31993)
                     /locus_tag="NBL02_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            32110..33189
                     /locus_tag="NBL02_000129"
     CDS             32110..33189
                     /locus_tag="NBL02_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCD2570845.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            33221..33814
                     /locus_tag="NBL02_000130"
     CDS             33221..33814
                     /locus_tag="NBL02_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD2570846.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(33924..34151)
                     /locus_tag="NBL02_000131"
     CDS             complement(33924..34151)
                     /locus_tag="NBL02_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCD2570847.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(34254..36842)
                     /locus_tag="NBL02_000132"
     CDS             complement(34254..36842)
                     /locus_tag="NBL02_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HCD2570848.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(36862..37521)
                     /locus_tag="NBL02_000133"
     CDS             complement(36862..37521)
                     /locus_tag="NBL02_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCD2570849.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(37570..38073)
                     /gene="trmL"
                     /locus_tag="NBL02_000134"
     CDS             complement(37570..38073)
                     /gene="trmL"
                     /locus_tag="NBL02_000134"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCD2570850.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(38294..39160)
                     /locus_tag="NBL02_000135"
     CDS             complement(38294..39160)
                     /locus_tag="NBL02_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCD2570851.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            39269..39910
                     /locus_tag="NBL02_000136"
     CDS             39269..39910
                     /locus_tag="NBL02_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCD2570852.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(39972..41333)
                     /gene="mgtE"
                     /locus_tag="NBL02_000137"
     CDS             complement(39972..41333)
                     /gene="mgtE"
                     /locus_tag="NBL02_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HCD2570853.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(41351..42250)
                     /locus_tag="NBL02_000138"
     CDS             complement(41351..42250)
                     /locus_tag="NBL02_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCD2570854.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(42252..43049)
                     /locus_tag="NBL02_000139"
     CDS             complement(42252..43049)
                     /locus_tag="NBL02_000139"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HCD2570855.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(43027..43731)
                     /locus_tag="NBL02_000140"
     CDS             complement(43027..43731)
                     /locus_tag="NBL02_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HCD2570856.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            43862..44437
                     /locus_tag="NBL02_000141"
     CDS             43862..44437
                     /locus_tag="NBL02_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HCD2570857.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            44555..45232
                     /locus_tag="NBL02_000142"
     CDS             44555..45232
                     /locus_tag="NBL02_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HCD2570858.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(45311..47119)
                     /gene="pepF"
                     /locus_tag="NBL02_000143"
     CDS             complement(45311..47119)
                     /gene="pepF"
                     /locus_tag="NBL02_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HCD2570859.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSDAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(47295..48293)
                     /locus_tag="NBL02_000144"
     CDS             complement(47295..48293)
                     /locus_tag="NBL02_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HCD2570860.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(48519..49172)
                     /locus_tag="NBL02_000145"
     CDS             complement(48519..49172)
                     /locus_tag="NBL02_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HCD2570861.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        14782 a        10392 c         8720 g        15562 t
ORIGIN      
        1 taaactatgt atattcttta atattttttg agaatgcact tcgtacataa taaactttaa
       61 tcacattagt ctttcatacc tacgatcgat ctctttgact cgcacccata aattcataat
      121 agtttgtcgg cgtctttgga cgtttcgaag atgagctacg cattctcttt ttttatagac
      181 aaaaaagata gagacgaaca tggagcttcc aaatttggat atttgatcca attttggaag
      241 ctccatttat ttcgtgtctg tctttttatt ttttccagct ttcgactaat tcttggttct
      301 ctttgatcca gtcttttgct gcttgctgtg gatcttttcc attgttgatt tccaacataa
      361 ctttttccat atctttttct gaccaatgga aattgtctaa tactttgtaa gcttctggct
      421 ggtcttcttt caatcctttt cgaaccatcg tatggatctg ctcttcgctt cccattccgt
      481 tttcaggatc ttctaagtat ttcagatcat atttggcaaa catccaatga ggtgtccatc
      541 ctgtaactac aattggttcg tgttttttga tcgcttgacc tagtgcaacg gtcattgctc
      601 ctgaagaaga tgtctctact ttccagtctt tcaagttgtc atatttttgg atcgtgtttt
      661 cagcagccgt aactacacca gctcctggct cgattcctat gattttctta cctgcctgat
      721 cggttaactc atcgatcgaa tttacatcca tgtaggctgg cactgctaaa ccaacttttg
      781 ctcctgtcaa attggcacct aggtcttcga cttgatcttt atattgtgca tattgtgaac
      841 catgagtttt aggtagccaa gctgaaacca tggcatctga ctcgccattt gaaacagatt
      901 tccacatgat tgaattatct aatggcgtca tcgttacatc atagcccatt tgtttcaata
      961 cttcaccgac tacatttgtt gaagcaactt ctgtatccca ttcaacataa gataagttga
     1021 tacgttttgt ttctttggca gatgaaaaac tccctatacc aatcgcacct aagatcacta
     1081 ctactacagc accaatgatc agtccttgac gttttttact tttctgtgtt gttgctcctg
     1141 cagctttttt attgtttggc tggttcaaat gctgtgtaaa acggtcaatg atgattgcta
     1201 agataactaa tgcaaccccg ttgacaaatc cattcccgac ttgtgctctt tgcaatgctg
     1261 ataatacgcc gcgtcctaag ccgggtgcac cgatcataga ggcaataacg accattgaaa
     1321 gtgacagcat tgttgtttga ttgatccctg ccatgatcgt acttttagct aaaggcaatt
     1381 ctagtttgaa tagtttttga cgacttgtgc tcccgaatga atcagaagct tctactaatt
     1441 ctttaggaac ttgtcgaatc cctaaattcg taaaacgaac ggttggtggc agagcaaaga
     1501 tgactgaggc aaatacccct ggaaccatcc cgataccgaa aaatgcaact gctggaatca
     1561 aataaacgaa tcctggcatt gtctgcataa agtcaaggat cggtttgata atactttggg
     1621 ctttactgct ttttgccatc aagatcccta atggtacccc aatgatgatc gagatcacac
     1681 tagataaaag aacaagtgtg acagtattca tcaaatcatt ccatagacct tggttataaa
     1741 taaaccatag accgattaat gtgaacaacg taagtcctgg acgtttctta gaaataaaga
     1801 aagctgctac cgtcaataac aagataaaca aaggagctgg aatcactaac agtaaatttg
     1861 taatccccga catcactgcc tgtccaatcg tttgtaaaaa tgaaaatagg ccagaaaaag
     1921 ttgtcgtaaa ccattctgtg atattttcta cccagtttgc cactggtaat tgatagttca
     1981 atagattatt catgttcact cacctcgctt gtttctgcta atgcttcaat cacactacct
     2041 cggatgacga caccttttaa ttttccatcc tctacaactg caagtggagt cggtgagtca
     2101 taaataatat tgaaaatatc acttaccacc atgtctttcc caatcaccgt cacattttca
     2161 tctaggaagt cgatcaatgg acgtttttgc ttcctagctt ctaacgcttg atctgcagtg
     2221 atgatccctt tcaggtgtct ctttctgtca acagccatca gcatgctgac ttcttcatta
     2281 cgcatccttg tcaatgcaac gtttggtcca tcagattcga tattcgtcgt caatgcaggt
     2341 accatgatgt tttgagccgt aagcactttg gaacgatcga cttcttccac aaattcacgt
     2401 acataatcat ttgctggatt ggtcaatatt tcttcacctg ttccgatctg catgatttcg
     2461 ccgtctttca tcaaggcgat tctgtcccca atccgtaaag cttcattcaa atcatgggta
     2521 atgaaaataa tcgttttttg aacattggct tgcaggtcca gtaattcgtc ttgcatttct
     2581 cgccggatca gcggatcaag agccgagaac gcttcgtcca tcagcaagat ctcaggatca
     2641 tttgctaaag ctctggctag gccaacccgt tgttgcatcc caccagataa ctgactagga
     2701 tattggtctt taaatgaaag caaactagaa ttttctaatg ctttttctgc tttttcttgt
     2761 cgttcttctt taggtacacc acggacttct aacccgtatt ctgtattttc caagatcgtt
     2821 cgatgtggaa acagaccgaa gttttggaaa accatattga ttttatgacg acggacttct
     2881 cgcaattctt ctttgcttaa cttcgataca tcttctccat caatatagat actgcccgaa
     2941 gttggctcaa tcagacgatt caatagacgg atcaatgtgg atttcccact cccggaaagc
     3001 cccatgatca cgaagatttc tccttctttt acgtcaaaat tcacgtcata cacaccaacc
     3061 gttgcaccgg ttttctttaa aatctccgtt ttcggctggt gatcattcat catatctaaa
     3121 gcggcttgtg tctttttacc aaatatcttt gttaaatgtt ttacttctac ttttaccaaa
     3181 caaattctcc ttttttatgt aaattcattt ttctttctga aaaaagtgac cacactatgt
     3241 taacatcatg tagtcacttt gtcaaaaatg aaagaaattt gcttgtccaa actgaaaaaa
     3301 acttggctaa atctttgtaa ataaaaggtt tagccaagtt aatatttttt taatatttca
     3361 cacaatcatt ctgcaagctg atgctccggg taaaaaaagt ttgtcatacg ctgtagtgta
     3421 tattcatttc caataaaatg aacgatgtcc cctgccgtaa atttagcata ggggccaggt
     3481 gaaacaagca gttcttcatt atgagaaatt gcgataactg tggcgccagt cgcctgccag
     3541 atattcaact cgctaattga acggtccaga tgcattgctt tttctgataa agataattca
     3601 aacggaagta aaggattcat cttatgtatt cgcttcgttt gattgaccag ctgatccaat
     3661 aattgagaaa aatgattcag ctcatcttgt tgcttctcca cacttgccat gatttctgct
     3721 tttgtatctt ggatggattg tacatcttga tattgcaata ggaaatcttt tgctttttcc
     3781 ttcgaagcaa caaatgcccc acttccatgc cgtacttcca tgattccaag atctaccagc
     3841 acattgatag ccttgcgggt agtctcagga gaagcattgt acgtactggc aagtgttgag
     3901 cgggcatgga tcttctcacc cactttatat cggttttcaa cgatacgttc ggctaaatca
     3961 acagcaatct gctggtagcg cgggcggacg atttttttct ttccagacat tttctttcct
     4021 ctttcttttt ttactttccc tcatcttact atgctctagc aaaataagca aaaaagggga
     4081 gaatagcctc ttgcaacgac tgatcatcgc ttgatctgtt ccttttttac tgggcaagct
     4141 gtttgacagt catcacaatt cttaccggat tttaaacgag tatagacaat tgcgcctgca
     4201 cttccaaaga ttaacacact taataagaat gtcgccatcc tattcctctc cttctaatga
     4261 caatatagta taaaactggt tcttggattt tggctgacgg aacaaaacat aaacgagtcc
     4321 tatcatcagt aaaatagcaa gatacgtccc taacgctacc tgtccaccct ccaataagtg
     4381 tcccaactga taaatcacaa aactaactaa ataagctaaa ccgcattgat agcctatggc
     4441 gatccaagtc catttcttgg cattcatttc ccgatggata gccccaattg ccgcaaaaca
     4501 aggcgcacaa agtaaattga aggctaacaa tgaataagct gctgcgggag tatatgtgtg
     4561 ttgtagcact tgccagattt ctctgccatt ttcggatact tccgcatgac caaacaaaat
     4621 accaaatgtg ccaataatat tttctttagc gaccaatcct gtaatcgctg cgactgttcc
     4681 ttgccagttt ccccaaccaa gcggctgaaa aacaactgcc aggattcgcc cgaaaaaggc
     4741 taagatactt tggtcttcag aaacaggttg agcataaaaa ttataagaag agctgaacca
     4801 gatcacgata ctagaaacaa agataatcgt tcctgctttt ttaacaaaag acttgctgcg
     4861 gtcataggtt tgtcttagga ccgctgacca ttgaggaaaa tgatatgcgg gcaattccat
     4921 gataaaagga gctgtatctc cagcaaacaa acgtgttttt ttcaagctga ttccagagaa
     4981 aataattgca gtcataccaa gaaaataggc agaaggagct acccaagatt gatgaggaaa
     5041 aaaggcccct gctactaagg caataatcgg caactttgca gaacaaggca tgaaggttgt
     5101 gaccatgatc gtcaaacgac gatcttgctc gttttcaatc gtgcgactgg ccatgacgcc
     5161 tggcactcca catcctgtag caatcagcat aggaatgaag gattttcctg aaagaccgaa
     5221 tttcctgaaa agtcgatcca tgacaaaagc gatcctcgcc atatatccgc agtcttccag
     5281 aaagctcaag cataaaaaca atataatcaa ttgcggtaga aatcctaaca ctgctcccac
     5341 gcctgcgatg atgccgttca agatcagctc ctgcatccat cccgctactt gccattcagc
     5401 taacaatctg ccaacatgtt ctggtaacca tgtaccaaac aatgtgtcat tgatccaatc
     5461 tgtccccatt gttcctactg tttgaatagc cagataatag atcagccaca tcacaaatgc
     5521 gaagatcggc aaggctaacc aacgattcgt tgcaatctga tcgattttgt cactcatcga
     5581 taattggaaa gaatcatttg acacaacaca gagtgtacat agttgggtaa taaattcata
     5641 acgttcattg accagaatcg tctcactatc gtcgtccaat agtttctctg ttaactggat
     5701 cagttgctcg atctcttttt gctgagaaac tgctatctcg taatcctctc ttaccttctg
     5761 atctcgttca aaatatttca ttgcatacca gcgtttcttg ccgctttgtt ctcctagtac
     5821 atcttcaatt tcatgtaaag ctgcttctaa acgtggatca tatgtacagg gaacaggaat
     5881 agaaggccgc ttttttgcag actcctgaat cttttcgatt agtttttcga ttccttgctt
     5941 tttcaatacg ctgattggta aaactggtac accaagacca taggaaagct tttctagatt
     6001 gatttttttt ccttcttttt tgatgacatc catcatattc agaccaatga tcataggaat
     6061 gtctaaatcc aataactgcg tagttaaata aagatttcgt tctaaattgg tagcatcaat
     6121 aatgttgatg atcagttcag gctgttcctg aaccaaataa tctcttgcaa tcacttcttc
     6181 tggtgtataa ggagacaaag aatagatgcc gggtaggtcc tgaatatgaa tgttcggatt
     6241 ttttttcaac gatccttctt ttttttctac tgttacgccc ggccaattcc ctacatattg
     6301 attcgaacca gtcaagagat tgaacaagct agttttcccg ctgtttggat ttccgactaa
     6361 cgcgatattc attctttttc ctccctttgt atcaagatta gttcggcctc tgactttcgt
     6421 aacgtcagtt cataccctcg cactgtcagt tcgattggat ctcccagagg ggctagtttt
     6481 cggataacta tttttacatt tcgcgtcaat cccatatcca ttaaccgtcg cttcactgcg
     6541 ccttgtccgt ggattgctac gacgatccca gtctctcctg cttttaattg atcaagagaa
     6601 gtccagcttt cagaggaagc agtcttttta ttgacaaaga tttgacctag tatttcccga
     6661 cttactgcca ctcgatgact ttttaccagt aaaatggcat tgttgcctga taaattgacg
     6721 acgacgattt tacttccggc aacgatcccg atattttgca aatggcgttt tgttttttct
     6781 tctgtttcga ttcgttcgac ttgataaacg aaattctgct ttgcctgcgc taaggtctgc
     6841 atattgattc ctctcttgtg aaataaatca tgtattaaaa tcattcatta tatgttctaa
     6901 ttttcttacg tgttacgcaa ataaatgaga atcattctca catagaatac tctcggtaat
     6961 tgagaatgtc aattatactt gtgatattat gagcgaatct ttagtattga tagataaaaa
     7021 tgagaaaaaa atatttcttc atagttttcc acaatttttt ctctattaaa tttgctattt
     7081 tcccacacga tacttgtttt cagctctttt ttcaaaaaca caaaacttag tattccacag
     7141 tttccacaac acaatatgta gtgtttgttg tttgactttt ttactaagag ttttatacta
     7201 tctattatag ataataagaa tgtgttagag gaggcactca ccatgaacgt aaaaatcttt
     7261 tctaaaaata attgtatgca atgtaagatg gtcaaacggt ttttaacaga aaatcaaatt
     7321 gcttttgaag aagtcaatat cgatgaacag cctgaagcag tagactggtt gaaagcacaa
     7381 ggattccaaa gtgttcctgt cattacttct gatgcagaaa cagttatcgg attccgtcct
     7441 gatcagttgc gtaaacttgc cagctaacat gactaaaaga atcaaaacgt gatacatgga
     7501 tttgaagggc ttgagacagc accagttttt gtcgcaaact tccaaatcct tttttaaagc
     7561 ttaaaaacaa tgcaatgatt tgcaatcaag catcatcaaa tacgaaatga aggttgggcg
     7621 tacgtatgag tcttaaaaat ttacaagatg tcagttattt caagctgaat aatgaaatca
     7681 atcggccagt caatggtcaa atccctttga ataaggataa agaagcttta gttgcctttt
     7741 ttgaggaaaa tgtcaaacca aatacgatga cttttctatc tattatggaa aaaatcaatt
     7801 acttgatcga tcaagaatat atagaagcag aattcattca attgtattca ccatcattta
     7861 ttgaaaagct atatcaattt ttggacgagc aacatttttc tttcaaatct tttatggctg
     7921 cttacaaatt ttattctcaa tatgcgttga aaaccaacga cggttccgct tatctggaaa
     7981 cttatgaaga tcgagtagcc ttcaatgcgc tttactttgc gaacggggat gaagaattag
     8041 cacttgctct tgcagacgaa atgatccatc aacgatatca gccagctaca ccttctttct
     8101 tgaatgctgg acgtaaacgt cggggcgaat tggtgtcttg tttcttgact caagtcactg
     8161 acgatatgaa ctctatcggg cgctcaatca actctgcttt gcaattgtcc cgtattggtg
     8221 gcggcgtagg gattacttta tcaaatcttc gtgaagccgg cgctcctatc aaaggatatg
     8281 agggtgctgc aagtggtgtc gttccagtca tgaaattatt cgaagatagt ttcagctatt
     8341 ctaatcaatt aggtcagcgt caaggagctg gcgtggttta tttgaacgtt ttccatccag
     8401 acattattgc tttcctttca gcaaaaaaag aaaatgcaga tgaaaagatt cgcgtaaaaa
     8461 ctctttctct tggcgtgatc gtacctgata aattttatga attgacacgt aaaaacgaag
     8521 atatgtattt atttagtcca tatagtgtcg aaaaagaata tggtgtccca ttctcttatg
     8581 taaatattac agctgagtat gatcatctag ttgcaaatcc aaacattcgc aaacaaaaaa
     8641 tcaaagcgcg tgatcttgaa aatgaaatct caaaattaca acaagaatct ggctatcctt
     8701 acgtaatcaa tattgataca gcgaaccgcg agaatccaat cgatggcaag atcatcatga
     8761 gtaatttatg ttcagagatt ttacaagttc aaacgccttc tgtgattaac ggcaaacagg
     8821 aatatgaagt attaggaacg gatatctcat gtaacttagg ctcgacaaat atcgtgaatt
     8881 tgatgcaaag ccctaacttt ggtcaatctg tgcgtacgat gactcgtgca ttgacattta
     8941 ttacagatgc atctgatatc gatgtcgtac caccgatcca aaacggaaat aagctgaatc
     9001 atacgattgg attaggtgcc atgggcttac acacttatct tgcaaaagaa caaatggaat
     9061 atggctcaga ggattcatta gactttacaa atatttactt catgctgttg aattattgga
     9121 cacttgttga aagcaacaat atcgctcgtg aaagaaaaca agttttccat aattttgaaa
     9181 aatcagctta tgctgacggc acttatttcg aaaaatacgt aactggagaa ttccagccaa
     9241 aatcagaaaa agtcaaagca ttgtttgaag gcatttttat cccaactgct gaagattgga
     9301 atgctttgaa acaagcagta atgaaagatg gactgtatca tcaaaatcgc ttggcagtag
     9361 caccaaatgg ctcaatttca tatatcaacg atacgagtgc aagtattcat cctatcactc
     9421 gcatgatcga agaacgccaa gagaagaaaa tcggtaaaat ttattaccca gcgccttatc
     9481 tatccaatga gacgattccg tattacactt ctgcttatga tatggacatg cgtaaagtaa
     9541 tcgatgttta tgcaatggca caacaacacg tcgatcaagg catgagtttg actttattca
     9601 tgcgttctga aatcccagaa ggcatttatg aatggaagac aacaacgaag caaacgacac
     9661 gtgatttgaa tattttaaga cattacgctt tccacaaagg aatcaaatcc atttattacg
     9721 tacgtacctt tactgatgat gcagaagaaa tcggaagcaa tcaatgtgaa agctgcgtga
     9781 tctaatatca tagatagatt cgcttattca tattttaatt aaaggagcat tctcatgtca
     9841 gaaacatatt atgaagcaat aaactggaat gaaattgaag atatcattga taaatccact
     9901 tgggagaagc tgacagagca attttggcta gatacacgta tcccactttc aaatgaccta
     9961 gatgattggc gtacattgtc tcaattggaa aaagatacag taggacatgt atttggcggt
    10021 ttgacactgc tagatactgt ccaatctgaa agtggcatgg atcagttgcg taaagatgtc
    10081 cgcactcccc atgaagaagc cgttttgaac aatattcaat tcatggaatc cgttcatgcc
    10141 aaaagttatt cttctatttt tagtacactg aatacgaaaa aagaaatcga agaaattttt
    10201 gactggacaa atacgaatcc ttatctgcaa aaaaaagctg agcgtatcaa tgaaatctac
    10261 aaacacggta caccgctaga aaagaaaatt gccagcgtct ttttagaaac attcttgttc
    10321 tattcaggtt tttatactcc tctttattat ttaggaaaca acaaattggc taacgtagca
    10381 gagatcatca aactaatcat ccgagatgaa tctgtacatg gaacctacat cggttacaaa
    10441 ttccaattag gattcaacga gttacctgaa gcagaacaag aaacgttaaa agactggatg
    10501 tacaatcttc tttatgagct ttacgaaaac gaagaacgtt atacagaaga attatacgat
    10561 ccaatcggct ggacagaaga agtcaagaca ttcctacgct ataatgccaa caaagcattg
    10621 atgaacctag ggatggatcc actctttcca gatactgcca atgatgtcaa cccaatcgta
    10681 atgaacggga tttcaacagg cacaagcaac catgacttct tctcacaagt cggaaacggc
    10741 tatttgttag gtcatgtaga agcgatgaaa gacgacgatt atttgattgg attggaataa
    10801 ggatttattt taattcaaaa atttttcgaa aaataaaaag agtttagcgt acagatattt
    10861 taaatgtaca ctgaactctt ttttatgctt attcacttgt ttcttccagc acttttggac
    10921 tgtctaactt ttccgaaaca tgttccattc ttctcaataa atagattttc cggatattta
    10981 atattatcgt cttaatcact gcatagacag gtatgcaaat cagcatgcct aatattcctg
    11041 cgacacttcc tgctccaatc aataccaaga tgatcgtcaa aggatggata ttcattgatt
    11101 tcccaaataa caacggcttg atcaaatttc catctaattg ttggatcact aaaatcgaca
    11161 acgccatata caaagcttga aatggagaga taaacaaacc gacaatgatt gctggtaccg
    11221 ctccaataaa agggccgaca tacgggataa tattcgtgat tccgcaaatg accgcaagca
    11281 acaagctata aggctgatcg aatattctca ttgccaaaaa gctcattaca ccaataatca
    11341 aagcatctaa gatcgtactg ctgatatagg cagataatgt ttcgtttaat tcttttaccg
    11401 tttggcgtaa ctctttacga atcgctttcg gaaagaagtg agacaacgca tcaataaatt
    11461 tgtgcccatc cttgaacata aatagcaaaa taaatggaac ggtaaataac aaaataaaaa
    11521 attgcatcat aaatgaaatg atttttgaga cacttgcaga taaactgact aatatgatat
    11581 tgaacaaatt actaagagat aaatttgctg atgctaattg ttcttccaaa ttgaaatttt
    11641 taaactcttc ccgctgcgcc aattcattta accatttgct tgtttcatct gcatacattg
    11701 gcaagctctg ggtcaattga attgtctgtt ccattaactg aggcactaca ttcatgacag
    11761 ttaaaacaac taaaatgatt acgaccaaaa acgtcaataa aaaaccgaac actctcggga
    11821 tccgatgctt ttcgagaaaa acaacgattg gatcgaacat ataatagata aaaccagcaa
    11881 caagcaatgg taaaaaaact gccgaaagca ttttcaatat tggactaaaa atattcggca
    11941 tttgtaaaat aaagtacacg ccgatgatcg tgagtactag ctccactgtc cagaaaaata
    12001 attttgattg tttaaaacgt gattgcatac atctcctcca aggtcctttg atacttctat
    12061 tctatcattt aacaaagagt tacgcgctga gattttcatt tgcttaaaaa aaacaaaaaa
    12121 aataaacata ttttaatgag tagacgagta cttaaacaaa aacacggttt ggagtagtga
    12181 caaaaattat actgcagtcc ctttttccaa ataaacggtg ttcaaaagct tgcatcttcg
    12241 taataagccc agccaaacga aaaatataag gaactatttt tatttggctg ggcttattac
    12301 ttgatgtagg acatctattt tacaacctca gatcatttat tgcgcaatac tgactaaatg
    12361 caactttgtg atatttttgt gtacggacaa ctcctcgatc acatcggcat aagtgagccc
    12421 ttttggaaga tcaatcgtat agatattttt gtacactcgg tagtcatcaa cgaattttac
    12481 gtcaaaattc acatcttcga ttacgatatt tttctcttca aaataatcgg taagaaaatc
    12541 tttggtttct tttctatgga taaattggat ttctaacttt ttagtcgtag gtacatggat
    12601 cacgcgtttc actactgtga gtgcaatagc aatcccgata aaactgatga tcgcaatgga
    12661 atagtaaccc atgccgatgg cgatccctaa tccagcaatt gcccataaag aagcggcagt
    12721 cgtaagtccc gtcaccgatt gtttcgtgac gatgatcgtt cccgcgccta agaaaccgac
    12781 accactcacc acttgagcaa tcagccttgc ttcatctgaa cgaatcgttc cagcaagctg
    12841 tggatactct aaagcatctc ttaatgcacc actagcgatc tcaacttgga tcaatgcaat
    12901 gatacatgct cccatacaaa ctaaaatatg cgtacgcata cctgctggac gatttttata
    12961 ttgtcgttca aaaccgattg ctccgccaaa tagcatagcg atccctaaac gtaaaataat
    13021 ctctggaatt gtcaatacca tatcattcat ctaaacaccc tccagtccaa tatatcatgt
    13081 ctgaaaggag cagacaaaaa aagtgcaatt tttacctctt acgaaaaaag gactgtgaca
    13141 aaatttttgt catagtcctt tttatgaata aacggtgttc aatcagctga ccctcggtat
    13201 tactcaacaa acggcaaaat atggggagct atttttcctt tgtcttctct aatactcagg
    13261 tcaaaacgct gatttcacaa cctctttagc ctactattga aaattacaac ttacttgctg
    13321 cttcttcatc gatgatcaca acaacattgt cgtgattttg caatacactt gctggcaaat
    13381 gattcgttac aggtccttca atcatccctt tgattgcatc agctttgctt tcgccaaatg
    13441 ccatcaagac gatttcttta cttttcataa ttgaaccgat tcccattgaa acagcttttg
    13501 ttggtacatc ttctgcttta tcgaagaatc gtttgtttgc attgattgtt gattctgtta
    13561 agtcaactac tgaagtcgtt ccgtcaaatg aagcaccagg ttcattgaaa ccgatatgtc
    13621 cattttgccc gattcctaag atttggatat cgattggatg tgcatcgatg atttgatcat
    13681 actttttgca tgctgcttcc agatcttctg cttttccatc tggtacaaac gtttctttga
    13741 atggtttttt atcaaataat tggacatcca tgaaatgacg gtagctttgt tcatctgaac
    13801 cgcctagtcc aacgtactca tctaagttta cagaagtcat ttcactgaaa tccaaatcgc
    13861 ttgaagtcat ttctttatat aaagtaactg gtgtgcttcc tgttgccaat cctaatactt
    13921 ttgcaccttt gttcatacct tctttgatga tttcaaatgc tttttttccg ccttcttcag
    13981 cgttttttac acgaatgatt tccatagtga ttgctccttt atattttggt atagcccaat
    14041 tatagcattc tttttttctt tttacaataa ttagtataga ccaaattgca tttttttcat
    14101 tttcttgaca ataaatagga atccgtgtta ggtttcataa gatggttcaa tcaaacaatc
    14161 catgttgcat gaaaaccaag caagtgattc actgtggacg ctttatgtct gcagtttttt
    14221 tatgaaaaag gagttattat gagtatactg acattaaaca acattaccca gcaatttggc
    14281 gacaaaatcc tctatgaagg tgtcgatctg caattaaatg ctggcgaaca tcttgggctg
    14341 attggacaaa acggagccgg taaaagtaca ttgattaaaa tcatcactgg tgagatctta
    14401 ccagatgacg gacagatcca ttggcaaaaa aatattcaca aaggttacct cgatcaatat
    14461 gtggaagtcg atgaaacatt aacaatcgaa gaatttctaa aaactgcgta cgcaaaagaa
    14521 tacgaaaagg aagaaaaaat tgcttttctt taccaaacat atagcgagac cttcgatgat
    14581 tccttgctgg aaaaagctgg gaaactccaa acggaattag accaaggagt gttttatcaa
    14641 atcgacacct tagttgctga gatgagcagc gggttgggga tcgatgtctt aggtttagat
    14701 tcactgttga aaaatctaag tggtggacaa cgttcaaagg tgattttagc aaaactactt
    14761 ttagaaaacc cagatgttct gatcttagat gaaccaacca atcacttaga tgatcagcat
    14821 atccaatggc tgacgcagtt tcttcaggat tttgacggtg cgtatatcgt gatttcccat
    14881 gaccaagaat ttcttgatca gatcaccacc catatcgcag acatcgagtt tggaaagatt
    14941 actaagtata ctggccatct aaaacaagca ttgaaacaaa aggaacaaaa tcgcgaaagc
    15001 tatttgcgcc aatatcatgc tcaacaaaaa caaatcgaaa agaccgaagc ttatattcga
    15061 aaatacaaag ccggttcccg atcaacgatg gcaaaaagcc gacaaaaaca attagataaa
    15121 atcgaacggc tgacgcctcc agcttcagca agtaaagctg tctttgattt tccattctcg
    15181 cctattgtca cgacattagc agtggaaaca actgatttag tcatcggata tgatcgaccg
    15241 ctacttgcac ccatcaattt gaccatccgc tttggtgaaa atattgccat tcgaggcttc
    15301 aacgggattg ggaaatctac tttattaaaa acactgatcg gtgagattga aaaaataagt
    15361 ggcgatttcc acttcccaga taacacaaaa atcaattact tttcacaaga tctgtactgg
    15421 gaaaatccat tagaaacacc actacaatat ttaagcagtc aatttccaaa agcgactatc
    15481 aaagaattac gtcgccaact agctaaagca gggttagtta atcaattagc aagtgaacct
    15541 ctcactacat taagtggtgg ggaacaaact aaggtgaaac ttgcacagtt gacaatgaac
    15601 caaggtaatt tactgattct cgatgaaccg acgaaccata tcgatcaaga aacgaaagaa
    15661 agcttacaag acgggataca gaaatttcca ggaacagtaa tcatcgtctc tcatgaacaa
    15721 gaattttatc aagatcttgt gcaacgagtc attgagattg aaccaaaata aacgtttgat
    15781 tgaatagttg aaaaaaagga gctgtgacaa aagttttgtc acaactcctt cttctgaata
    15841 aatggtggcc aattagctga ccttcggtat tagctcaaaa aacggcaaaa tatgaggagc
    15901 tatttttatt tggctattct tattacttga tgcagaacag cttttcacaa cctcttttcg
    15961 tctatttttt attttcgaga tttctctttc ctttaaaaac aactgtttga aagaagaacg
    16021 cacatccttg aaaaacaagc ccgctaaccg ttacacctaa ccagccatac tgctgccaca
    16081 ttgtcgtccc tagcaatgaa ccagccgcac ctccaataaa gtacataaac ataaatacag
    16141 tattatttcg gttgcttgct gcttcaccta gcccttgtac tcgtgtttga ttggcgactt
    16201 gaccaaattg attcccgacg tctaataaaa taatagctaa aagcaaaatg gccaaatggc
    16261 ttccgccaac caataaagca ataaagctta ttgtttgcat aacaagtccc atatacacga
    16321 tttttctttc agaataagaa tctgccagtc gtcccacgat aggtgcagca actgctccag
    16381 acaaaccaaa aatcgctaat atccctgctt cgaatgttcc ccaattgtag gcgggactgc
    16441 tgatataaaa aatcaacgta gaccaaaaga tcgaaaatgt tccaaacatg aaaaatccac
    16501 tgactgccgc ttctcttaat agacgttgag aagcaactaa atgcggcaaa cttttaagtg
    16561 aatcaaggta tgaaacagct gtactacttt gctggacaga acgcggcatt ttccagtaaa
    16621 gcagaagtgt aagaattccg actgcacata aagcaaataa atagatcatc cgccaagaaa
    16681 aaagactggc aacaatacca gataccgtcc gagaaagcaa aatccccacg agtaaaccgc
    16741 taagcaatgt acccatgacc ttccctcttg cttttggtcc cgctagtacc gcaccgtaag
    16801 gaatgatgat ttgcggcacg atcgaaagta atcctattaa aaaagaagcg catgcaaata
    16861 acgtaaacga aggcgcaaaa aaagcggcca acagagagac ggcagataac gctgccattc
    16921 gaatgatcaa ttttggacga tccaccacgt cccctaaagg aactaaaaac agtaacccta
    16981 acgcatagcc taattgggtc aacatcgtca atatcccgac tgcacttgtg ctaacagata
    17041 aactagctgc tacttttgtt ccaatcggct ggatgtaata catattggca acaacaacgc
    17101 cacaagtgat tgataatagt aaaataagcg aagttgttaa aggttttttt tcattactct
    17161 tcattgattt cccccatttc ctatcttagt ttacaactta tggaaaagat tggaaataga
    17221 aaaatacaac tattcgccac acttattatt tgtgcttttg ttttccttgt tttttcttgc
    17281 gatctttata aatgaagaca actaacccta ctagcacagt aacacttgca accaacgtga
    17341 ctgcagtttt gttatatccg tgaaacagag cttctccacc acacgcatac aacacagacg
    17401 aagggatgac tccaatcacg atcgacaata tgatttgctg tagaggcaat ttcatcgcat
    17461 ctgcagcaaa attgaccata gaagatggaa taaccggtat catatagcct aacattacac
    17521 ctatttttgg atgtttcatt tgtcggattg cttgcaccca atgattcgtt tcggttttct
    17581 tatctaaaaa ttttaagcga tgcataagga aaattgcagt gatgttacca agagcattac
    17641 ccaaaatgtt gatgatcgca cctgtcagtg gtccaaagct caaaccagca atgactccaa
    17701 ctactgaagt aggaatacct ggaacagcac acatcaccgc tgtcaatacg atcaagagta
    17761 tcgatgcaaa agtcccatgc gaacgaatat gtgcgagtag ctctgcctta ctcgctctcg
    17821 gatttaaaaa taattggatg tctgtctgat attctaaata caaccgatag cccactacca
    17881 gtatcagaat gattcccaac gagatcaata tcaatcggat tattttcttt ttcatatttc
    17941 ctcctgaaaa aaatctatat tcattaaata atagtttaac ataaaaacaa aaaagcaccg
    18001 aaatccaaca taggatctca gtgcttttaa atagtaaaaa ttatttaaca gttacagatg
    18061 cgccaacttc ttcaagttta gctttcaatt cttcagcttc ttctttagat acgccttctt
    18121 taactggtgc aggagcgcca tcaactacag ctttagcttc tttcaagcct aaaccagttg
    18181 cttcacgaac tgctttgata actttaactt tttggtctcc agctgcagtt aattctacag
    18241 tgaattctgt ttgttcttca gcagcagcag cgccaccagc agcagctaca gctacaggag
    18301 cagcagcaga tacgccaaat tcttcttcaa tagctttaac taggtcgtta agttctagga
    18361 tagttgcttc ttttaactca gcaacgatgt tttcaatgtt caatgccatt ataatttcct
    18421 ccgtttaatt gttctttttt atttaatgct ttatgcggca attaagctgc atctccatct
    18481 tctttgtctg ccactgcttt gacagcgtaa gccacgttgc ggactggcgc ttgtagtaca
    18541 gatagcaaca tagatagaag tccttcgcga tttggcaatt ttgccaatgc tgtgatttct
    18601 tccaatgaag agactttacc ttcgatgata ccgcctttga tttctaatgc ttttgcttct
    18661 ttagcaaatt cgtccatgat tttcgctggt gcgactacat cttcgttact gaatgcaaca
    18721 gctgtaggtc cagtgaatac ttcgtccatg ccttcaaggc cagctttttt agctgcacgt
    18781 gaaagaatag aatttttgat aactttcatt tctacgttag catcacgaag ttgtttacgt
    18841 aaacgtgtta cttcatcaac tgttaatccg cggtagtcaa cgactactac tgatgcagct
    18901 gctgtaaatt tttcgcttac ttcatcaacg attgctgctt tttttgcgat aattgcttca
    18961 ctcatttttg agtttcacct cctgaatttt gatggaagag ttttttgcac tgaaaaggca
    19021 agtcactttt atagttactt tgctcaacca gtattattca tcacctattc acttcgttca
    19081 tagtgattct actcgctttg ctcgaaagga aactcctttc gctttgctca accagtattg
    19141 ttcatcatct cttcacttcg ttcatagtga ttcacaataa aaaactctat gccaccgcag
    19201 acatagaggg actattgaat atattcaata aacgtcctcg gtaggaaatt aaggcttgat
    19261 gccacctact gtcttcggta caagttaatt attaacctca actagcttag catgctaaac
    19321 tagttgagtc aagaattatt tttcagttgg atcaatcaac attctaatat ttaattagaa
    19381 agaagcttgg tcaacatgga taccaggtcc aaatgttgtt gtgatcgtta catttttcat
    19441 gtattgacct ttagctgctg atggtttagc ttttagcaat acatcatgga ttgctgcaaa
    19501 gttttcaact aatttttcat cgtcaaatga aacttttccg attggtacat ggatatttcc
    19561 agctttgtcc acacggtaag ttactttacc agctttcact tcgtttactg cttttgttac
    19621 gtccattgta actgtaccag ttttagggtt tggcattaag ccttttggtc ctaatactcg
    19681 tcctagacga cctacagtag ccatcatatc aggtgttgca acaactacat caaagtcaaa
    19741 ccatccgttt tggatttttt gaaccatgtc gtcgtcacct acgaaatctg caccagctgc
    19801 ttcagcttct tttgctttgt ctcctttagc aaaaactaaa acagtttgtg ttttaccagt
    19861 tccgtttggt aatacaacag caccacggat ttgttggtct gcttttttag ggtctacgtt
    19921 caaacggtat gcaacttcaa ctgttgcatc aaattttgca atgtttgttt cttttgctaa
    19981 agctactgct tcggcaacag gataaacttt acttgaatca acttttttta atgcatcttg
    20041 catttttttg ctctttttag ccattttggt tcctccttga ttgtggttat aacggttgta
    20101 cctcccacgt gttctatatc tttcaatata gagccaactg agaagctgct gtcttagggt
    20161 gtatagatta ttctacagtg atccccatgc ttcgtgcagt tccttcaacc atgcgcattg
    20221 ctgcttcaac gttagctgcg tttaggtctt ccatttttaa ttcagcgatt tctcttactt
    20281 gatcgctaga aactttcgca actttatttt tgtttggttc gcctgaacct ttttcgattt
    20341 tagctgcttt tttcaataga acagcagctg gcggtgtttt tgtaacgaat gtgaatgaac
    20401 ggtcttcgta tacagaaata acaaccggaa tgatcaaacc tgcttgatca gctgtacgag
    20461 cgttgaattc ttttgtgaat cccatgatat tgatacccgc ttgacctagt gcaggaccta
    20521 ctggtggagc tggagttgct ttacctgcag gaatttgcaa tttaacgatt ttttctactt
    20581 tctttgccac gagacatacc tccttaagtc cgtgatgtgg ttaattggag atgcatattt
    20641 ctcctcccac gttttatctg atctgaaaaa atccagacaa ataatgtgca aaacaatgca
    20701 cactggtaca gtatagcaaa tttatttgtt ttttcaagat ttttttctgc tatttttaca
    20761 ttttgcttga ataaacgcta aatttattct aaaaaaacaa caatttttcg ttctcatttc
    20821 ttcttttatg ttacacttgc gctagcatgg ttcaacagat gaaagcaaac aataacacgg
    20881 ttttttagac ccttgtacaa agattcctac ataacaaaat gaggtggaga aaatgaaaga
    20941 tttaaggtat agaagtgtct ttgatattat tggacctgtg atgattggtc caagtagctc
    21001 tcacactgcc ggagctgcta gaatcgggaa aatcatacgt agtatatttg gcgaacagcc
    21061 tgattctgta gatatttatc tatatgaatc atttgcaaaa acctatcggg ggcacggaac
    21121 agatattgca ttagttggcg gcctgttagg gatggaaccc gatgatgaac gtttagctga
    21181 ttctttgaaa ttggcatatg aagccggtat ggaagtcttg tttgttccta agagtgaaaa
    21241 agccgaacat ccaaactctg taaagatgct tctaaaaaaa ggaacacgaa agctttcagt
    21301 cactggcatt tcgatcggcg gtggcaatat ccagatttct gaattgaatg gattcaaaat
    21361 ttctttaagc atgggtactc ctaccttgat cgttgtccac caagatgttc caggtatgat
    21421 tgctaaagtg actagtatac tttctgagac caatacgaat atcggcacta tgacagtgac
    21481 acgggaatca aaaggtgaaa atgccattat gataattgaa gtagatgatc ctcaagtaga
    21541 agaaacggta aaaagactca aacagctgcc aaatatcgac agcgtaaatt atttcgatta
    21601 aggagctaaa aaaatgtttt tatcgattga agaattagta gatcaagcaa aagcttatcc
    21661 aagtgtagca gaattgatga ttgcagttga aatggaaatg agtggccgtt cccgtagtca
    21721 catcatcgag atcatggaaa aaaacctctc agtaatggaa caatcaatca acgagggaaa
    21781 agaaggtgtt acctccgtca caggaattac cggtggtgat gcaaaaaaat tagatgatta
    21841 tttaaatgag gcagactttt taagtggaga aacaatctta aaagctgtac gcaatgctgt
    21901 agctgttaat gaagtcaatg caaaaatggg gttgatttgt gctacaccga ctgccggaag
    21961 cgcgggtgtt gttcctggtg tgctgatggc tgtccgagat cggcttgatt tgacacatga
    22021 acagcaattg gaattcttat ttaccgctgg cgctttcggg ttagtcatcg caaacaacgc
    22081 ttctatcagt ggtgcagaag ggggatgtca agcagaagtt ggttctgcaa gtgcaatggc
    22141 agcagcagct ttggtttgtg caaaaaatgg gactcctcat caagcagcac aagcagtagc
    22201 cattacattg aaaaatatga tgggactgat ttgcgatccc gttgcaggct tagttgaagt
    22261 cccgtgtgtc aaacgtaatg ctttaggctc ttctcaagct tttatctctg ctgatatggc
    22321 attagcgggt atcgaaagtg tcattccgcc ggatgaagtc gttgctgcaa tgtaccaagt
    22381 cggacgtcaa atgccgtcga ttttcaaaga aacagcagaa ggtggcttag ctgttacgcc
    22441 aactgctaaa cgacttgcgg aagagatttt aaataagagg gcgtgataaa gctccttaat
    22501 ttcgagtatt aagagattag ttatcttcat aacaaaaaac gaagagagga ctaattgccc
    22561 ttctcttcgt tttttattaa tccatcaatt tcgtattttt tacttcatcg atcgtttttg
    22621 ttcctgcaag ttgcatcgtg atcgacaatt ctttgttcag gtgttcaaaa acagatgtta
    22681 ctccttctgc cccacctaga ttcaacccat aaatcactgg tcggccaatc gcaactaagt
    22741 cagcaccact tgccagagct ttgaagacat gctctcctcg acggacacca gagtcgaaaa
    22801 tcactggtac acgtttgttg actacttcgg caatttttgg caatacttca aaggacgcag
    22861 gacctccatc tagttgacgt ccaccatgat tggataccca gataccatct gcccctgcag
    22921 agatagcgat tactgcatct tctggcgatt gtattccttt tacgataact ggaagatggg
    22981 tgatttcttt gattgttttg atgtcatctg gtgtcaagcc ttgttttgca gctgcataaa
    23041 tttctgcgat tccttttcct tcgccattac cacttgcact ttgttcgcta tacgcagcta
    23101 agttaggcat tggtaatgga aattggaatt gattgatgac gtcttcttca cggtaaccac
    23161 cgagcgttga atctgccgtt aaaatgatag ctttagcccc tgcttctact gctttgttca
    23221 aaatgaactc attgaatctg tcgtctttac tcatatataa ttggaaaaac tgcggtgcgc
    23281 ctggcgctgc atcggctgcg tctttgattg tcgtatttgc ataagtgctg atggaaaaga
    23341 ttgatccagc agcagctact cctttagctg tatcagcttc ccctttttca tgtgccaatc
    23401 cttgggccgc tgatggcgct tggataatcg gtgtatctaa ttcaattccg aaaacagatg
    23461 tatgcaaatc ggctgagtcg attccgcgta atatccgtgg catgattttc tttgtattaa
    23521 aagaagtcgt attttctttc atcgtccact catcttcaga accaccgcga atatagccaa
    23581 aagctcctgc ttccatgcgc tcttttacac gaccttctag ttaggcaatg tttacaatat
    23641 cgattggatg ttcttcatta cttgtatgat aagtcatagt agatccttct ttcatcttct
    23701 tgattacata taaatcatac taaacttaca aaaagtaagc aaacatttac tctcagatac
    23761 ttttatcgct attctttcca aataaatcga cttacaagct gatctacttc ttggttttca
    23821 tgaagcatac tatgagaggc attttcacct gtcactactt tttcctctat ttcatttccg
    23881 cgttgcttca acaacgcata gaccgctaat gcactgttta acggtactgt tccatcactc
    23941 aagtctgttt catctagttg accagcaatc aagaaaaatc gagtggttat aggaatatta
    24001 ccgatcattt gctggtaatc ttgatagcgg ttagaaacga cagcaggccc ctttgctaac
    24061 tcatctgtca gagactgcgc actatcatcg acaaaatcat taaaaggcgc gccaattgct
    24121 gttaatgtct gaacttgtgg caatgacgta tcctgtccat acgttcctaa atagcgtagt
    24181 gcactaactc ctcccatgga atgccccact atgttgactt tatctatgcc gtagttttct
    24241 ttcagataca aaagacaagt tttgatccat tctgcttgat tccactcgtt atttttatta
    24301 tcttcaaaca atacttggac catcggattg tctgcttttc cagacaaatc tcctgttgct
    24361 tgtatttgtc catctgttcc tactgttaaa accaattctt ttactgcttg ctgatttttt
    24421 tccaaacgcc gcaacatccc tttaaaggaa ttttctgtcc cgctgtagcc gtggaaaaaa
    24481 agtgttggaa tagtggtttt ttctatcact ttactggatt ctgccgtttt ttctttatca
    24541 gaaacttcag tagtatttat cttcgtcgtc tggcaaccac ttaacgcaaa caaactgata
    24601 aggactaaag ctgctgcagt tgcacgtttg cataaatttt tgattggttc tgtcatacac
    24661 ctcttccttt ccatatagaa aaaagaaaga cttgaaaggc caatcatatg atcgacattt
    24721 caagtctctt tattaatcga ttttatctac ttgatcgaaa tcaagttctg tactggtttc
    24781 acgtccaaac atatcgatgt tgactttcaa tttttctttt tctccgtcaa cttctgttac
    24841 ttgtccttct aatccagcaa atgcgccttc gatgattttc acgacttcgc caacttctac
    24901 ttctagatca ttttgtcgca ggctcatacc gatagaacgc aagatatgat tgacttcttc
    24961 tggaagtaat ggtgctggtt tgcttccggc tccgtgtgaa ccaacgaatc ctgtcacacc
    25021 aggtgtgttg cggacaacat accatgaagc gtctgtcatg atcatttcaa caagtacgta
    25081 tcctgggaat gttttatgaa cgatttcttt ttctttccca tttttgactt cagtttcttt
    25141 ttcttcagga acaacgacac ggaaaataaa gtcggccatt ttcatacttt gcgcacgaga
    25201 ttcgatattt gcttttactt tgttttcata tcctgaatat gtatgcaaga cataccagtt
    25261 tttttcaaaa gattccattg aaggctcctc atttcattgt aaaataaaaa aacctcagta
    25321 cacccgaagt ttttctttcg cttcattata gcactaaaag tacgttgtgg ctagcgagtt
    25381 attttaaaat ccagccaaat aagctttgga tggctgtatc cattacgtaa aacatcaatc
    25441 cgaaaataat agaagtttcg atgactacta acgtatcttt acggagttgt tttctgcttg
    25501 gccaagttac ttgtttgatt tcatctttta cgctttttag aaatttcatg agacaacctc
    25561 ccttgctact gtttatttcg tttctttatg taatgtatat tgattacaat atttacaaaa
    25621 cttattgatt tccaaacgtt cgccgctttt tccttcactg actgattttg tgtagttgcg
    25681 agacccacaa acggaacaag cgagtgctgc ttttttcttt gccatttact tctgccccct
    25741 tgctgagaat cagacttata cttcataaca gtatcacgta actttttact tgtcaatttg
    25801 tgaaataaac tatctattgt tttggataaa attatgatat gattaatttg aaattaagct
    25861 aaaatgagac aattggaggt atagcgatgc tattaaaaac acttgtatat aagaaaaaag
    25921 atctgacaac cgttacagag actagtacat tagaagaagc attaaaaatt ttagaaactt
    25981 ccggataccg ttgtgtgccg atcttagatt cttcaggtaa tattttccgt ggaaacattt
    26041 acaaaatgca tatctatcgt cataaagcaa atggcggtga tatgacgctt cctgtcactc
    26101 atcttttgaa aaacgctaca aagttcattt acctggatac ttctttcttc aaagtattct
    26161 ttacgatcaa agagcttcct tatattgctg tccttgacag tgaaaatcat ttttatggta
    26221 ttttgacaca tagtacgttg cttaatatgc tctctcaatc ttggagcgta gaacaaggaa
    26281 gctatgtact gacaattgct tccacaggaa agcaaggaga tttggctgct attgcaaaaa
    26341 tcatttcaaa acacagcagc attgccagct gtattactct tgatatcgaa aaagacgaat
    26401 acattcgacg tacattgatc acgctaccgg ctaacactga agcgaaagta tgtgaaacga
    26461 tcgtcaacca cttagagaaa aagaattata aagtggtaga aattgaaaat ttgcaaaacc
    26521 aagaagcata atcaaaaagc gaaaaaagaa aacagctaat ctgctttctt ttttcgcttt
    26581 ttataattag acacgctttt caaaatcaag cgcgcgatct aaataataca tcaatggtgc
    26641 tgaaacagcc atgacgataa actcacttaa tgcaagcgtg ccataggttg tccaaaatgc
    26701 ttggccacct gttggaacta acatataagc aatcagtccc atgctgatgg taaagaagat
    26761 cgtgttcaat actaaacgca tctttacatt tttcactttg ttttgcaata aagctgtcag
    26821 gcctaaagaa agcaatgttt ggccgccacc aaaaaccaca tccatgatac caaaaccaaa
    26881 gaaggcatta tagacgatga ccccgcctaa aactccccaa agcaatttac ggttaaagac
    26941 aactaaatga ttcaaacttt ctgaaatacg aaactggatc ggccctgagg atacaggcgc
    27001 aactaaaata gttaacgcta gataaagagc catgatgatg ccatttacaa cgattacgga
    27061 taaccgttgt ctgcttttcg ttttttgcat tttctctcct cttttctcct agttttttta
    27121 acgcgggatg gttgatgaac cgcggttatg tgaaacaaac aataattata acagactttt
    27181 catttatttc aatccgtctt atctgctttt aaaaaagcaa aagcttaaac aatgtaaacg
    27241 tttgctaaaa atcgtgggat atggtataaa gtaagtgtaa ggggtcataa gaattcccag
    27301 taaagcagac gaagtaggat ctgcaagaac agaattgttg tgaaacttaa ataaataaaa
    27361 aaggatgtgg gaatttgagc aacctcttac tagagaacgg tctagcaagc agctgaagat
    27421 ctaagtggac atgccgaacc ttggaacttc tccttacgta agtaatggat catgcgcttg
    27481 ctaggctttt ttgtgttttt cggtcttcgg acctttttca ttttcctaga cttctgctat
    27541 aataaccaag aattaatttt gtaacagatt tttttgaaaa aacaagtttc tttcattcaa
    27601 aaaatagaaa aagcttgcta attattggtt aaccagcaaa caggagtatt aaaatatctt
    27661 gttttattga atagcaggag gaaatcatga aattccatta tcatatattg atcaatccat
    27721 cggcaggcag cggaaatggc tataaagtag cagaaagaat tttaccggta ttaaaaaata
    27781 agcatattga ctatacactt cattatagtg aatacaaagg acacgatgct gagattgctg
    27841 aaactttggc aaaagaaaca ttgattcctt ggatagaaga agaacagaaa acaatcgata
    27901 ctttccccct tttgatcatc gtaggaggtg acggcacctt acatcaggta cttgatactt
    27961 tctatcagat ggaagtagaa ttcccagttg cttatattcc agcaggttct ggagatgatt
    28021 ttgctcgagg ggcaggcctt ccaaaaaatc ccaaaaaagg acttcagctg attttagcag
    28081 ctcaatcacc agaaaaagtc cacataatgg cttatgaaga aaaaataagt gaaaaaaaag
    28141 gaatagctgt taataatttc ggtataggat tagatgcagc tatcgtccat gcgacaaatc
    28201 attcttccac aaaaaagcga ttgaacaaat acaacttagg atcattttcg tatttattct
    28261 ctattctccg tgctttattc actcaaaaag gatttccaat tctggtagat gcaggaggaa
    28321 aacaattaag tttttcaaac gcatttttat gtacagcaac gaagcatcct ttttttggcg
    28381 gaggaatcgc gattgttcca actgctgatg cgtctaaacc agtcattgat tttgtcgtgg
    28441 tcgaacgaat caatttcttt aaaatcttgt ggctcattct tttacttata cagaaaaaac
    28501 aattgaaatc gaaatatttc catcactaca caacaaacag attacgtatc gtttcaacaa
    28561 caccgcaaca tggtcaagaa gatggagaag aaatggaaaa acgacctttt gatatcacaa
    28621 taaccacaac taaacagttg ttttggtgtt aataaaagag gttttgtcag aagctacatc
    28681 tggaacaccg tttattcgga aaaaaggact gtgacacgac ttttgtcaca gtcctttttt
    28741 tactatgcag ttaattcttt gcccaattgg gttgccatcg atagtttctt tctttaccca
    28801 aaataaattg gatcattgaa aacaccgcag caaaagcaac taagacacaa ccattgaacc
    28861 aaaaagattc tgcaaatgtt tgattgtaac tgagtgtttc actgatcaag ttattatttt
    28921 gaacaaacca atatgttggt gtgaatgccg ctattttgct aaccatttct ggcaagtatt
    28981 ctgctggtac aaacacccca ccgataaagc aactgcctgt gataaaaata tctttgatcc
    29041 caccgctggt ttctgagttt tttaccaatg aagtgataca agccgaaaag ctaatgatcg
    29101 gcaataaaaa caagaaagta tttaaaatat gataccatac cgttatatcc cacgaggtgt
    29161 ggatcactaa cagaaccatt agaagtaaga agccactgaa caacagcagc gagtaactaa
    29221 tcaaactaaa ggttactttg cgattcaagt ttcgtttggt aatgggcgca ctctggtttc
    29281 gtttactgat ctctttgcga ttaaatgcta gattgatgac gctgaaaccg ctaaaaatgg
    29341 atagaaacaa cccataagac aagaaattaa aggttgtgcc cgtcaacagc tttttcatcc
    29401 gctgactata tcccgcactt agctcaactt ctccagtaac cgagagtgcc atttgtgtct
    29461 gttccatagc tgcatcgatt gttttatctt cttcttgaaa cagaatcagt gtttgcaaaa
    29521 aggtgttcac ttgctgtgta acaactgttt tagaaaaggc atctgggcga ctctgtatct
    29581 tgatcgtcgg catctttcct acttcgactt gttcggtaaa gtctgcaggc agatgtaaga
    29641 taatgtttac acgattaaaa taaagagcat catcgatttc tttttgtgag gttccatcca
    29701 atgtaacgac tgtttccttg tcattcaaat atttacgaag gccatcgact acaggcgact
    29761 cttcttcact aatcaaggcg attttcgcat cttgcagaga ggtattttca acatcaatat
    29821 tttgcgcaaa aaagaatgtg gcaacaacag tgataattac acctatcagt aaattcgatt
    29881 tatttgcttt aagcaatgtc caaatcgttt tataaagcgt cataacgcac ccctctttcc
    29941 atccaagcat ttcctaacgc aaaaaggaaa ataaatccga tcaaccatag aagattggta
    30001 taaaaactag ccaaatcagt gtagaaataa agtttataca aactttcgct gatcaagttg
    30061 acgagattta attgaccaac aatcggaata tgaagatcaa tccaatactt cacttgttcc
    30121 gaccccatca taccagcaaa aaagctcatc gcaatcgtca ctgaaatcaa gatgctatcc
    30181 ttttgcacca gattcatttt tggtatcaaa ttaccaacca atgtacctaa caaaatcgca
    30241 cacaacgacc ccagagcggt aacaatcatc aaccattgcc aacgttcacc aaagtctact
    30301 tgatagataa aacgaaagac agctaacata atataaatct ctgtcaaaac aatcgtaaag
    30361 cttgctgcca aattagcgat tgaaactaac gattgctttc taggaatcaa ggacaagcga
    30421 ataccatttg acgattgatt tgcttgctga tcctgtacat tgcggacccc ccacatcgtc
    30481 ccgtagagaa tcgccattcc aatcagagtg aagaaataaa aacttttgaa actcatatta
    30541 cgacctgcat gaacttcttg aaaagatact tcttgttcaa aaacagtcat gatgggcgcg
    30601 agatcaacgt tctccacttc tgccacttta ctcgaaatta taccaatacg ctgtaaaaat
    30661 tgatccatga cgtttttcat caacgtctga ctcattcctg cttgacccac atggagcgaa
    30721 acagatgaac catcaatgag ataaaaacca gccaaatcat ctgcatttag cttttcattc
    30781 gcttctactt ccgatagttc ctgtacttca aataatggtt tatcattgac ttcgacttgt
    30841 tctaagactg tggtaaaatt ttccgaaagc gctgtttgat ccgctttgat tacaattcca
    30901 gtattaatcg tttcgagatt gccaccttga tcaatctcgc caaaggccgc cataaacatc
    30961 aatcctaaaa gaattggaaa ggccaatgtc caaaacaaca tggatttatt tcgcagcagt
    31021 atttttgtgc gatatttata aaaatgccaa aacattattt cccctcctct agtctctcaa
    31081 atccttgcca gtaatctcta aaaagacatc attcaaggac gcttctttgg ttgctatttt
    31141 tccgtaactg atcttttctt cttgtaagat tgtaagaatc ggcaacaata ctgtatttag
    31201 ttcagtagtt gttacttgga gctgaccttt tttataagcg attccatcag aagctattgt
    31261 tcgctgacaa cacgttttct gggagtaatg ggatgtcgat cgtcaaacga tccgccgtcc
    31321 gtaccatatc ttttagagat tctttggttc ctttagcaat cacctgtcct tgatccatga
    31381 tcacgatctg gtcgcaaaga atttctactt cttccatgta atgtgttgta tagacaatgg
    31441 ttgctccttg tcgattcagc tccttgatac tttctaaaat tttattccga ctttgggggt
    31501 ctacagctac tgtcggctca tctaaaaaaa ttaaccgcgg cttgtgggca atcccacatg
    31561 cgatgttcaa ccgacgtttc aagccaccac taagttctcc tggacgaaac gaaacatatt
    31621 tttctaatcc cactaattga atcacctctt caatcaaggc tttccttttc tctttttcgg
    31681 aaacatataa accgcaaaaa taatcgatat tttcttgcac acttagttcg tcaaagaccg
    31741 caacgtcttg aggaacgacc ccaatcattg ccttgacagc ataattttca ggagtcattg
    31801 ttgtcccgaa caatttaatg gttcctttgt cataatttaa caaagaaagc atacagttga
    31861 tggcagtcga tttcccactg ccgtttggtc ctaacagacc taagacttct ccttcttgta
    31921 cttgcagatc aaaatgattc aatgctacaa aatctccata gcgtttgact aatttatcaa
    31981 tttcgataat catcatgaaa aactccttcc aaatttcttg ttgatctgac tataataaag
    32041 tgcaagaaaa gaaactagtg acgattggct aaaccacaca tgacatttgt cacaatgaag
    32101 gagtctgcta tgcataaatc tcggctactc aacgaaacta gctttctact ggttatctca
    32161 ttgcttttgc tggtaagtca aggattcgtt gctatgcaag ttatctattt attgatcgcc
    32221 gttttattca ctggtgtcag ttatttactg cctacaaagc caaattggat tttccaagtt
    32281 attctatttc ttttatgtgc ctttttacct gatcttgtcc tgtttctgcc agcgacgatg
    32341 cgagtgatcg cccctgaaga aagccacctt gattacttgt ttttgttagg ttttttgttg
    32401 atttcgacgg ttggtaatca tccccccatc aaggtcgcct ttgtcgtaat tttgctgttt
    32461 ttttcctttt acttacgaaa aaaagatcaa cgaacgaccg atcttgagaa aaagtacatt
    32521 gtctccaaag atactcattg ggaaaaacaa caagagttat cggaacaaaa taaagcattg
    32581 gccctgtcac aagaaacaca attagccttg caaattgtgg aagaacggaa ccgaattgcc
    32641 cgagacatcc atgacaatgt cggtcatctg ctgtcaagtg cgattttaca aattggtgcg
    32701 cttgaaatga tcaatcaaga accaaaacta aaagaaccat tagaaaattt aagtgccacc
    32761 gttcacactg ggatggatcg gattcgtcaa agtgtccatg acctccatga gacatcgatc
    32821 tcttttcaac actccttaga gtttctgatc gctgattttc cttcttctgt tacgattgaa
    32881 ggaaacttgt ttgaaggatt gaatgaaacg cagcaaaact gcttcttaat ggtcatcaaa
    32941 gaagccttaa ccaacagtat caaacatagt catgccaaaa ccgttgtttt acattttcga
    33001 acgttgccgg ccttttatcg cttgcaaatc acgaatgatg gcaccagccc acaaaaaaat
    33061 tctgctcccg gtattggttt gaaaacgatg cgtcaacgtg ttcaagaaca aaagggacaa
    33121 gtccatattt tccaagaaaa ccagcaattt cagttaacga tcattttacc aagggaggga
    33181 caccaatgat aacagtagtc attattgaga tgatccattt gtgacgaaat cgttacaaac
    33241 cattttagaa agcacgaaag agatccgtgt gttaggcatt ggtcattgtg ccaaggatgc
    33301 gctagtctta tacgaaaccc atcgtcccga tgttttgctg acggacatcc gcatgcctaa
    33361 acaaacgggc atcgatgctg ccaaagaaat tttggctaaa tttcccaaag ccatcctttt
    33421 gcttttgacg acattcaaag atgaagaata catccgcgaa gcattttcca tcggtgtcaa
    33481 agggtattta atcaaacaga acttgcaagc gattattcct tctgtcaaat ccgcttataa
    33541 tggacaggcc gtatttggta atgaaatcat cgagactttc actcaaatga tgaagaataa
    33601 accgacgatt gatagtcatg cttatgcttc ttttagtgaa cgtgagcttg caatcatcaa
    33661 agaagtagct gccgggaaaa acaataaaga aattgcagat gccttgtact tgagtgacgg
    33721 cactgttcgc aattacatca gccaattact agaaaagcta gatttacggg atcgcacgca
    33781 attggctatc tatttttatc agcatttgca ataagtctta tctaacacaa aaaaacctga
    33841 atgatgctcc ttcgcatcat tcaggttttt ttcacatctt ctattcaatt gtcccgcaaa
    33901 ccatttcctc acgattgaaa gaatcaagga ttcgcagggc tgccaggatt cgttggattg
    33961 ttcggcgtgt taggcggaaa aataggcgcc ttgtctaacg ttttttctag ttttgattct
    34021 tcacgatcgt ctcgttctac atcaggactt tcattcaatt tgaattcttt ttccatctca
    34081 tctaactttt taaagtcatt gccagattct tttgctaatt tcgctaattt atcttcggtt
    34141 tctttagcca taaagtaacg cccccctttt gatttcagct tagcatatca cactgaaaaa
    34201 acaaaagaaa acgctaacca acgatcattt ttagctattt ctcatcttta aaatcaagca
    34261 ttttttgccg gtataaaatc gtagggagaa ctgttttcca taccaatcat cggatctact
    34321 aactggttat cgatcacttc tgctgtaaga acgaaatctt tagaaacagc aggttctacc
    34381 tcttctttct tttcttctcc aacaaatgaa gaaaacagat ctggttccac tgtttccatt
    34441 acttggtttt tcgtttcatt cgtcccactg tcatcttcta caggtagctt ttcttttaca
    34501 acccgtggct cttttgtata gttcccatct tcaaatggat catttccgac caaagaatct
    34561 tcataagctg ctagtttggt ccggagagtg atattcattt tatcattatc catgatcctt
    34621 gatttcaacg tagtcatccg gcttgctgat tcattcgcta cacaagtgac ataggcttgc
    34681 tcttccccta acaaaatcaa cagttcctta ctgcgagtca ctgccgtgta gagcaaatta
    34741 cgctgaagca tccgctgata ttgatggacc atgggcaaaa taaccatctt gaattcactg
    34801 ccttgtgatt tatggattga gcaacaataa gaaagagtga ttttattcca ttcgtttctt
    34861 ttataagtga cctcatttgc atcaaactgg ataactaatt catccacttt atcttcagaa
    34921 tcttttgcca agatgatacc cgtgatttct cccatatctc cattgaatac attcaattcg
    34981 ggagtattga ctaattggag gactttgtct cctatccgat aaacggtgtc gttccaatgt
    35041 acttcctttt ttccaggttt ccctggattg aatatctcct gcatcatttt atttaacgca
    35101 tcgatccctg caggtccccg atacattggt gctaatactt ggatatcttg tgcacggaat
    35161 cccttatttt tcgctttttc cacaatttta gagatcaagg gttcaatttg ataagcatca
    35221 cttcggaaat aggaacgatc tttttgattc tttgtgaaat cttttggcag acgtccttct
    35281 ttgatctcat gggcaagagg aataatacta gaaccatcac cttgccgata aatctgattg
    35341 agttccattt tgggaatttg ttcgatttcc aaaagatcat ggagtacttg accaggaccg
    35401 acagagggaa gctgatcttt gtctcctact aagataactt gcatattcgt cggaatcgct
    35461 ttgaataatg tgttagccag ccatgtatcg accatagaca tctcatcaac aatcaaaagt
    35521 ccgccttcta attctttcgt actgactgaa ggtgtttttt cccgtccatt cagccccaga
    35581 agacgatgga tcgtactggc aggcagacct gtcgtttcgt tcattcgttt agctgctcgg
    35641 ccagttggtg ctgcaagtag aataggaaaa atttcttggg tatattttga aggatccaat
    35701 tccagtccgt tcaactctgc aaaaagattg acgattccgt tgatcacagt agttttcccc
    35761 gtacctggtc ctcctgttaa aataaataga ggggaacgga ttgcttcagc gatagcttgt
    35821 tcttgcgaag gtccatactg tatgccgaat attttttcga tacgccgaat attcttctgc
    35881 acttcttttt caggataatg gatttctttt ttacgactga gaaggcgctg gatcgaatta
    35941 ccgatccccc attcagaaaa gtacaaactg ttttcgtaaa gattagtctc ttcttgttgt
    36001 atctttcctt cttcaacaag ttggataatt acagtagcaa cctgatcagg ctggatttca
    36061 attgggcgac ttgtttctaa aatacggatc gtttgctgaa gcaatgtctc cgccggtacg
    36121 tatgtatctc ctgtttgaac tgcatgctga aaaatctgat gcaagatagc tgctcgaatt
    36181 cttctcggtg aatctgctgc aataccgatt tgttcggcta gattatctgc acgtttgaac
    36241 ccgattcctt ctatgtcttc gactagctgg tacggatttt cttcgatgat atccaaagac
    36301 tcgttcttat aggcctggta gatactgaaa gctaactgac taccgaaccc gtaacgattc
    36361 agtcccatga tcacttgatc catcccgtgg tttagccgaa tcgtctcagc aagcatttct
    36421 ctttttggct tcgtcaaacc ggagatttgt tttaataccg aaggatcagc aatgattttt
    36481 tcgatagcat cttctcccag caattctaca attttttctg ctgttttctt cccgattcct
    36541 gggaattttt cgctggaaag atacgctacg atcccatttg cagttgttgg cttttcttgc
    36601 tcatatcttt cgagtttaag ctgccttccg tatttaggat gatccactaa ttctccataa
    36661 aaacgataag tctcattttc ttgaatatcg ccgaaacttc cggtgactac aatctctttt
    36721 tcacggtaat cagctgtggt ttcactgatg tgtaccaaaa gcactttgta aaaattacta
    36781 ggattttgaa aaaaaatcgc ggcaactgta ccaacgaaat aaggcatttc ttctggctgc
    36841 atcggaagcc gcctccttct tctatagtaa aggttctggt gctgtttcag ttcctaaaaa
    36901 atctgtttgg ttccatactt tcagttcata tggcaataat ctgcaaggct cttcagtctc
    36961 taaaatcgtc aaactactat tgaacaatcc acccatttca cgcaactcac ttagctcttt
    37021 tcctgtcatc cattgaatgg ctgcagttaa agaagctcca tgtcctacga ataaaagtgg
    37081 gccatcttta tgttgggcaa ccgcatctgc tacaacagtt tcgatacgtg tgattgccga
    37141 ttcaatcgtt tctccgtgaa aaggagaagg atcatacaga tctaaatgat tacgcaaatt
    37201 cggcagatta tccggatatt tctggtacat ttcatcgatc gactgacctt ctaaagtacc
    37261 cagccctagt tctcttagct tatctgtata aaaaatctct accggacgag caagctcttt
    37321 gttgatacct tctgctgtat cacgtgctcg ctttgctgta ctggaataaa ttgctttaaa
    37381 aggaacatat tttacctttt tcccaaatgc tttgatttct tcgtaacttt gtggcaaaag
    37441 cggagaatcg ccattgctcc cttgaaaacg tctttcctga ttccattgtg tcttcccatg
    37501 tcgggtaaaa tatagttcca tacgcagcaa actcctgtct ttcttttatt ttactctata
    37561 ataaactgtc taatccagct tgtcattttc atagtgatgc gtcaattcta aattagggaa
    37621 tccttgctgg cgaagtgctt catacactat cattgcagct gtattcgata aattcaatga
    37681 gcggacatgc tcatcattca tagggatgcg taaacacttt tcttcgtttt cacgcataaa
    37741 tgtttctggc aaaccggttg tttcttttcc aaataaaaag aagtgctctt ctccatcagc
    37801 aaaattttcg tcactatata ctttatgggc aaattttgag atcaaatgta gtttacgatt
    37861 acctaaaaat gttaaaaaag cttctaaatt actgtggtaa gtaatattta catcattcca
    37921 ataatccaac cctgcccgtt ttagatgttt gtcatcagtt gaaaaaccta gtggctcgat
    37981 taaatgaagc ggagtattcg tggctgcaca cgtacgggca atatttccag tgtttgcagg
    38041 gattaatggt tcaaataaaa cgatatgatt catcaggatc attcctctca tatttatgtt
    38101 cgtttctatt ttctgacaat ttgatttttg cataaaaaaa aacgtatcaa ctcggtgtca
    38161 gtcactgcga gtcgttacgt tagtgaagct cttctcacta acttttatca gaacaggaac
    38221 ctgataaaaa ccaatgaaaa aacaacttgt tgctaatata acaccctcaa cttttttttg
    38281 caacctctac tcattaaaaa ctgctagaaa aacgttttct cttagttcct tttaataatt
    38341 ctatgtcaat tgtaatcttc ttcaaaggat tcttatataa gtctgctttg attcgctctt
    38401 ctacctgcca ttctaaaggt gacatggcca aaagatcttt ttgattttcc tctggtatct
    38461 caaattcata ggttacacgc tgacgttcag ttaatttcat tgcttcagca aacttggcta
    38521 ctacacgttc attggaataa ctctgttttt caggttgatt cggataaaaa gcctgacgta
    38581 gttcctttaa ataattttct tgtggcacca ccttcaacac ttctccatct tctgagagta
    38641 cacgttggaa ttcttggtaa tgagaaggcg aaaagatatt taaaaccgcg ttgaatgttt
    38701 gatcagcaaa cggcaaattg gtcaaatcag ctacacacca aaatgcttcg gcagattgtt
    38761 ctgtagctgc gtagactcct tcttttgaaa tatcaaatcc aacagcattt tgaatctttt
    38821 tcaattttga aagttcattc aagaagctac cttctccgca gccaacatct aatagcgaga
    38881 cttcttcagg taaatgctgc gcaatagtct caataacggg ctgatacatg ccagattgga
    38941 tcattctacg ccttggaacg aacattcctt gatcgtattc tgttttaacc gcatgggata
    39001 aaaaataaag tgttcctttt ttggaaaggt caaaacggtg attatttttg catatcaagc
    39061 cattatcaga cttttttact tcttcttggc aaaccggaca gcggaacagc tgatgatttt
    39121 ctgccaaaaa ctggcgtcct cggtcaattt tttttaacat agttccccct catttctgtt
    39181 ttatcttacc gttagatgct cactttggca actgctaaaa atatgataca atgcctagaa
    39241 gaaagtgaaa gataaaggag aagattagat gatcaaagaa ttttgcgctg aaaactacac
    39301 atctattcct ctagcaattg ctagaggagc cgatcggatc gaattgtgtg acaacttagc
    39361 ggtcggtggc acaaccccga gtactggtgt gatcgaagaa acgttaagtt atgctgggga
    39421 aaaagatatc cccgtaatga cgattattcg tcctagaggg ggcgacttcg tctataacga
    39481 tatcgaattg aaaatcatgc atactgattt gattgaagca aagaaattag ggacggatgg
    39541 cgtagtattt ggttgtctta ctccttcagg ctggctagac gaagaggcct tagaactatt
    39601 gatcgaaaca gcagaagggt tgcagatcac cttccatatg gcttttgaca gtattcctga
    39661 agatcggcaa ttcgaagcaa tcgattggct ggtagaacat ggtgttgacc gtatcttgac
    39721 tcatggcggt cctgctgaca aaactattgt agaaaatttc ccacggctta aagaattgat
    39781 tgcctataca gatggccgct taatcatttt gcctggtggt ggtgtcactg cagagaatgc
    39841 agcacatgta gctaaagaat tgaacgtaag cgaagtacac ggaaccaaaa tcgtggatct
    39901 tcagttataa tcttataaac aaaaaaacat ttgcaacagg cataatgctt attgcaaatg
    39961 tttttgtttt tttacataaa atagcccata aataaagaag caatattgaa ataaatcagt
    40021 acactcgtca aatcgctcaa agtcgtgata aagggaccgc ttgctactgc tggatcaaat
    40081 cctagacgat ccattagcat cgggattaaa ctacctgcaa gattcgccac tgtgattgca
    40141 aatagcatag ccatccctat gacaaacccc aagataaaat tatgtttcca aaaacctacg
    40201 attagaaaaa tcgtgatccc cgtaacagct cctgtgacaa cgccggtcaa gacttcagcc
    40261 aaaacaagtc gaacaaatcc atgatctttc tcatcagaaa tcgcaagtcg acgaacagcc
    40321 acagctagcg actgggttcc agcattaccg gcagttcctg taatcagcga aatgaagact
    40381 gctaaaatac tcgcttcact taccaagtct tcatagtgac tgattaaaga agcggtcgac
    40441 atacctaaaa acaacagagt gatcagccaa ggcaaacgtc gagacgctgc ctttactgga
    40501 ttttcattaa tttcttccac attgaccccg gcaagtcctg aataatcgct agcagcttca
    40561 tcatcaataa cgtcgatgat atcatcgact gtgacgattc ctaacaaatg atcatcataa
    40621 tcggtaacag gaagagctaa gaagtcataa tctctaaatg tttgtgccac atcttcttgg
    40681 tcgtcgccta catgaactga aagcactcgt tcgctcatca gatcagaaat cattgcgtcg
    40741 tcatcattca cgattaggtc gcgcagagaa ataaccccta ctagctgacc ttcttgatta
    40801 acgacataga tgtaatagat tgtttcagct acatctgctt cgttcttcag cacgtacatc
    40861 gcagagcgaa cagtttgatt ggctacgatc gaaacaaatt cagttgtcat gatcgcgcca
    40921 gccgtttcat cctcgtaatg gagaagttcc ttgatttcac tggcatcgtc tgccgacatc
    40981 aaacttaaat atttagcaat ttgcttttta tccagcgtat taagcaagtc aacagcatta
    41041 tctgtataca tctcagaaag catatcagct gcatagctag gacgcatttc atctaggtat
    41101 tctttcatat gttcgttgtc ttcttcaatc acatcgaaca tatccgctaa ttctttaggc
    41161 gacagatagg aatagatcag ctgcctgttc tcttctgtaa gagattggta gaattgcccc
    41221 tgctcataga tatgcatctc taaaaaactg tttctgaatg cctgaatatt attttccctt
    41281 aaatcttgag tcaactgggc aaatctttcc tctaattctt gattttcatc caaaccaatc
    41341 tcacctcgcc ttattcttca aaccgtctca caactgcctc catgtctgcg gctaaaggct
    41401 gcttgaactc cagcaactct tctgtaaatg gatggtaaaa acgcagatca taacaatgaa
    41461 gggcttgtct ttttattccc agatccattc gcccgccata catatcatca ccgagcaggg
    41521 cacagccaat tgcggaaaaa tgaactctta tttgatgtgt ccttcctgta tgcagctgga
    41581 tatcaactaa cgcgatttct gttagtcgct tctctatcca gtactcagtc tgtgccgaac
    41641 gtccttcatc agagaccatt cgcttcaata gcgaagaata atctcgggcg atcggcaaat
    41701 cgatatgtcc atgtgccgat aaatctgaaa gccttccact gactaaggcc tgatattttt
    41761 tcacaaactt cttttcacga agttgcatat ctaattttgc gtgcgcaaat ccatgctttg
    41821 caaaaagcat caaaccagat gtatcccgat ctagtcgcgt caccacatgg attacttggt
    41881 tttcgtaacc ttgtttttta taataagcct ttacacgatt agccattgtc ccatttggat
    41941 ggtattgggc aggaatagac gaaacgcctg ctggtttatt tactaccaga acgtgttcat
    42001 cttcatacac gatatcgatc ggtgtttcat ccaaaagtac ggtttcatgt tctccttcag
    42061 caggaatcac aatggttacc ttatcatcct tagctagaga aaacaagaca ttttctactt
    42121 gatcattaac tttgatttga ccgccttgaa attttatttt cgctaacaga ccctttgaga
    42181 tccctttttc ttttaaaaaa tattttactt gctgagaatg atctttctca taatcccatg
    42241 taaactgcat atcaatcctc actaataaag gcatctttca cacgatgcca aaaatgcata
    42301 tgtcgataag aagcaaaatg gatccgttct tctgctattc gataataaat tgagcggata
    42361 tttgcttttg cgacattcaa ttgatcaacc gttacaagat aatcttctgt attttccaat
    42421 tttatttcta gccattcatc atgggcaatc acgatcggag aacccaaagt acgaaacact
    42481 cgattattca gtgaagcaat ctctgccaac tgaaaggcat tgatactagg atggatcaca
    42541 gcaccgccga cacttttatt atatgctgtc gaaccagtag gcgtagaaat cgtcaatcca
    42601 tctcctctaa aactttcaaa cagctcgttt ttgatgtata catcggctac catcgtacga
    42661 ttggcccgtt taatcgttga ttcattcagt gctaaaaaat gctggtccgg tgtttcatcc
    42721 aaataactga tccgaacatc cagcaaggga taactaacac tttgttcccg gttcgttcgt
    42781 aagctgtcta ctagttcttc cagctcgtaa tcccgccaat ccgtgtaaaa ccctaaatga
    42841 cctgtatgca cgcccaagaa acgcacatca ttcagacaat gactgtagtg atggaacgcc
    42901 gacaataatg tgccgtctcc gccgactgag atgaccaatt ctggctcatg atcatctcgt
    42961 tgaatccctg cttgttctaa aagtgcgttc aaacgttctg tcacttctat agtctttgct
    43021 tctttattat gaacgatcgc tactttcatc ccctgcctcc tctctttcgc tttctttctt
    43081 caaaagcgtt cgatagtaga catcatctga ttttcctctt ccatgggaga ataaccgctg
    43141 tgcttcttgg atttcttcac ggatactgga catctcttca tccaactgat aggcagcttc
    43201 tgccgcacgc tgaagtcgtt cactcatttc ttctggaaaa acgccttgat atttataatt
    43261 cagcgaatgc tcaatcgttg cccagaaatt catcgcaaga gtccgaatct ggatttccgc
    43321 caaaattttc ttctcaccgc tgatcagctg tacaggatac tcgatcacca gatgataaga
    43381 acggtagcca cttgcttttt tatttgtgat gtaatctcgt tcttggagaa ctttcatgtc
    43441 ttttctttta cggatgatct caacaacttg atggatatct tctacaaact gacacatgat
    43501 ccttaacccg gcaatatcag acatttcttc ttctaaacgt cccattggaa tatgccgtaa
    43561 ttgtgatttt gttacaatac tatcgactgg cttgactctc ccagtgacaa attcaatcgg
    43621 gacatgtcga ttttgttcac ggaactgttt gcggatcccc cgcagtttca ctttcaattc
    43681 tgatacagtt tgttcatatg gtgctaaaaa tgcttcccaa tctcgttcca tagctgatac
    43741 ctctacttca ttaacattca ttgtttattc taccatatat gcaaagtttt ttcgtactct
    43801 tgatttatgc attgaccaaa aaaagaaaat taggcaaaat agacaaaaag gagtcgatca
    43861 aatgacacaa atgcttgaaa tcgaatataa atctatgctg acaaagcaag agtacgagat
    43921 attcatcgct cactatcaat taacagataa agatttccgt gtacagacta atatttattt
    43981 cgataccgtt gatgaacagc tgaagaaaca aaattgtggc ctacggatcc gtttcgttgg
    44041 tcatcaagct gaatatacat taaaaacacc tgctgaaaaa gggcgtctcg aaacaacgga
    44101 tacctttata acagaagaag cagaagcatt tatcttcgaa aaacgtctgc ctcgttcagg
    44161 cgcagttttt caaaaacttt ctgatttgaa tatcgatcct gcttcattaa tccaaaccgg
    44221 aaagcttaca acaaaacggg ctgaattccc tattgaagaa ggacttttag ctattgatga
    44281 aagctgggga gacaatctac acgattttga actggaacta gaggtagggg atgcatctgt
    44341 gggaaaaatt gcttttataa attttttaaa acgtttttct ttaccttatc gtcctgctaa
    44401 gaacaagatc caacgtatgc ttgaagctaa aaattagaaa cctttcattt tcacaaggca
    44461 cttgagttca tttttttact tttttctgtt aagttatagg atgtgaaaga atctagtcac
    44521 tagttgtata gaatctattt tgggggagat tcatatgatc gaaatctatt tatttgttaa
    44581 tcctttaggg gaatactgtt tcgaaatgga acaacagtta ttacaattta tcgaagaaga
    44641 gtacgggaca ccatcaaaag aaaagatgca atttcgcttt cttcctttgg tcaatttgca
    44701 gacaattggg gatgtcatgc aacaaaaagg aatttctcag aacaatcttg aggaacgtaa
    44761 tcacttattc tcaatgactt attctgcagc attggactgc aaagcggcgc agtttcaagg
    44821 aaagaaaaaa ggccgccgtt ttttgatcaa attgcaagaa gcagtcggct gtaatgacat
    44881 accatattct caagaacttg ctgaatccat atttgaagaa atcggcggcg atttgaatat
    44941 gtttaaagaa gatcgtcagt ctgacttcgt taaagaagta ttcttttctg atcaaaaaat
    45001 tgctcgggaa atgggcatca caaaacaccc ttctgcagtc gtttacaatt atgcctgcga
    45061 ccgtgatttt ggtgttttag tagaagtaaa aggtacaagt agcctttgga gaatcacaga
    45121 gctgtgtaaa acaacagata actcatatca agtttttaat ggtgaacatt tacatacaaa
    45181 tgattctcag ttccattcaa gaactagcca tttacacttg ctttctaatt aaaaaaatac
    45241 gtcgactccc ttacaagaga gccgacgtat ttttttcgtt tctattcgag aaaatcaaaa
    45301 atagaaagcc ttataaacga tcgatcaatt cttctaattc atttagacgt tgttcaaaca
    45361 tcgacattgc atcttcaata tatgcagcct gtgtcatatc aactcctgct tttttcatta
    45421 cttccacagg ataatcgctg ttacctgctt ttaggtaagc caagtagttc tctagtgcct
    45481 ctggttcctg gtttagtatt ttcttcgcca gtgctgaagc agcagaaaag ccagtcgagt
    45541 attggaaaac atagtaatta taataaaaat gcggtatccg tgaccattcg aatttaattt
    45601 ctgggtcttc ttcgactgct ggaccataat attttgcatt caatttacca taactatcac
    45661 ttaggtattc actagtcaat ggcacacctt tttcatcttc cgtatgcatg aaatgctcga
    45721 attcagcgaa ttgtgtctgg cggaaaactg ttcctttaaa cccatccaag taatggttga
    45781 gtacataagc ccgtacacga gggtcttttt ctgtttccaa taaatactcc gttaggatat
    45841 tttcattcgt tgtcgaagca atttctgcta aaaagatgga gtagtcgccg tacacataag
    45901 gctggttcga acgagtgaaa taactatgaa cactatgtcc catttcgtgg acaagcgtaa
    45961 atagctgatc cagcgtatca tgccaattca ataaaatata tggatttgtg tcatagcttc
    46021 ccgaagaata agcatcgctt cgtttccctt tattttcgac aacatcgatc caacgttcag
    46081 agaatgcttt ttctacgatg gccatgtatt cttcacccat tggtttcagt gcttctaaag
    46141 ctttttcttt tgcttcttcg tacgtaaagg taattggagc ttcccctaag accggtgtat
    46201 aaagatcata catgtgcagt ttttccactt ctaataaacg tttccgtaat tccatgtatc
    46261 gatgtaacaa aggcaaatgc ttgtttacca cgtctaccaa agtatcgtat acactttcag
    46321 gaatatgatt attgctcaaa gacgcttctc tggcagagct gtaattacgg actttcgctt
    46381 taaaattatg tccttttata tgtgtgccta aagtagaagc aaatgtattt ctaaattgtt
    46441 cgtaaacact gtacaatccc ttaaatgctg cttcgcgcac ccttcgatcc gtgctttcta
    46501 gcaactgacc atacacgcca tgagataatt ggactatttc accattttct ccttcaatcg
    46561 ttggaaaaac tagatctgca ttattcaaaa cagcaaatgt atcacttgaa gcatcaaaga
    46621 tttcacctgc tccagcaaga agagattcct gctcagcaga taagacatgg gctcgattat
    46681 ctactatttg ctggatataa tggcgataga cttccaattt tggttcttct ttgaaatatt
    46741 gccaaatctg gtcatctgac aattgcaata tttctggttc aaaccaggaa acggcttcac
    46801 taactttcga aaacagactg cttgctcttg cataaagcgc ttggtaatct gtatttccag
    46861 tgtcttgatc gtttttaagg tgcgcatata cataaatgac ttcagtttgg cgataaaccc
    46921 tcaatacgaa ttcaatcgca tttaaaaatt gagaagcgcc ttgatcaagt gttcctttgt
    46981 gtttttcaga ttgttttaac tcttctgata attccaaata tttctcatca aactcttggt
    47041 cgcttgaaaa gatcttggtc aagtcccaag ttaaattttc aggcaattct tctcgttttg
    47101 gcaactgctt tacttccatg aaatccactc cttttcttcc cttagctcta tctatcaaaa
    47161 tagatcatag tctaacatcc acatgtcaca gcatctaact tttctactca ttcatgtgtc
    47221 tgcttccatc aattttagca taactaaatc ttgttttaca tagaaaaccg attcctagcc
    47281 tatttttata cttactattt tttcaaagaa gacaagtgta tgcattcgtt aaaaaactgc
    47341 tgatagacca tctcttcatc aatcatagga aaaagccatt cccttttatg ttcttttatt
    47401 tggcaaagcc atgagttgaa atcaggaact ttctggtttt ccaaggcttc ttcgtacaac
    47461 attcgataaa gaaaaacctg ttcttgtaaa taaaagaaaa accgactttt ttgatagatc
    47521 cacgatgata gatgcaaaag atgtttatga cgcaagtagc attgttcttg tatggatact
    47581 gtattctttt gcttgtgaat aagttggcgt gtcagccatt tcttcccatc ttcactcact
    47641 ttttctattt ttttcatttg gttcatttta atttcgttac tagtgaatat ttctttaagg
    47701 tccctactag aaaaagtcca gcagctactg caaaaggaaa tatgccctga tacagtctct
    47761 gtaatatgat gatacaaata aagcttttgt ttcttgagat cagctttcca gcaatgcact
    47821 cctctttttc tattatagta gcagcaactt ttttctatca atgagaattg cgattgtcca
    47881 agaaaagaat taccaagtat ccaccatact tggtaactgt tattcaaata attttgggtt
    47941 ctttcaataa atctctggtg agacagtcta gaacattgga tttctattgc tagattcgcc
    48001 aacaaaatat ccggcctttg ccgcaacctc ggcaaatagg cttccaaaaa tactggttct
    48061 actgactgtc ccagccaatc catgaagcat tcctttaact gtaagtgctc ttttgattct
    48121 ctttcactaa agaccgaaca gttatctgat ttttgatggg caaaatgagc acattttttc
    48181 tttccctttt tcaaaatcac tctttttcga cagccacgac aataatattt ctttcctttt
    48241 tctgcatgtt tagcaaaaca gcacttatta tattcattca ttgcaagcag catttttctc
    48301 cctctgaact tatttacgtc taagaattta tttacacgaa aaaagagggc gtgaaaaagc
    48361 tattctgcat caagtaataa gaacagctaa acaaaaatag ttcccaatat ttttcgtttg
    48421 gttgggcttg ttaccgcaga tgcaagcttt tgaacacatt tatacgaaaa aaagactgcg
    48481 acacaatttt tgtcacagtc tcttactata atgctgattt atacaaaata aaatttcgtt
    48541 cgctcgatag cattacgcgc catcagacat tgtccatatt cattcaatgt ttctggtgta
    48601 cgatcgctct tttcagcaaa ctccaaaatt cgagcaatct gattctctac atccgcttgg
    48661 ttgagatttt ccatccagaa gtgaacgact aggtaatagc gctcttcgaa taaatacaaa
    48721 tcagtccaag cagactctag ttcgacatct gttgcaagtt ggatcacttg ttcaaaatct
    48781 tcaaaagcaa aagttgcttg tctttcctgt tcttctaggg cgcttccaga taattcttcc
    48841 tccggatcgc tgccaataat ctgctgacga agaaagtcac tcacatcttc tgaatcaaca
    48901 gtagaatctt ctgattgaga aatttcttca tccggaagat ttttactgat aaacagttct
    48961 aatccgtctc cttttggcaa aacttggaaa gtgacagctt cgcttcctcg gaactcctct
    49021 tcgatatcaa cttcttccaa aatactataa aagaaatttt cgatttcttt gtgattgcca
    49081 agtaaatcca aaaatgaaac gccccgttcc tctaaatctt catgaccaat tagtacacgg
    49141 atcgtatttt cattaatatg ttccatttcc attttgctac acctcacttt gccacaatgt
    49201 ttaatataga ttcattgtag cgaaatcagc ttaaatgtaa agaaaaaaaa tctcgctgtt
    49261 aagcgaattt aataaaacca gcggattttt caacctttca agctatctta tttaactata
    49321 acatttttgg cagacgaata ctatatagcc gacttttcag ctaattttcc ctctctattt
    49381 ttcgtgcaat catcatcttc tactaccttt ttatacatct aatcaagaat actttttgta
    49441 aaaacaaaaa aacttg
//
LOCUS       DAHHZJ010000004        47813 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.004, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000004 DAHHZJ010000000
VERSION     DAHHZJ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.004"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(38..1189)
                     /locus_tag="NBL02_000146"
     CDS             complement(38..1189)
                     /locus_tag="NBL02_000146"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCD2570862.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="NBL02_000147"
     CDS             complement(1202..1639)
                     /locus_tag="NBL02_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCD2570863.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="NBL02_000148"
     CDS             complement(1654..3420)
                     /locus_tag="NBL02_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCD2570864.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="NBL02_000149"
     CDS             3684..4361
                     /locus_tag="NBL02_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2570865.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4364..5491
                     /locus_tag="NBL02_000150"
     CDS             4364..5491
                     /locus_tag="NBL02_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2570866.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5691..6131)
                     /locus_tag="NBL02_000151"
     CDS             complement(5691..6131)
                     /locus_tag="NBL02_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCD2570867.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6142..8175)
                     /locus_tag="NBL02_000152"
     CDS             complement(6142..8175)
                     /locus_tag="NBL02_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCD2570868.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8177..9880)
                     /locus_tag="NBL02_000153"
     CDS             complement(8177..9880)
                     /locus_tag="NBL02_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCD2570869.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9974..10924)
                     /gene="manA"
                     /locus_tag="NBL02_000154"
     CDS             complement(9974..10924)
                     /gene="manA"
                     /locus_tag="NBL02_000154"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HCD2570870.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10963..11988)
                     /locus_tag="NBL02_000155"
     CDS             complement(10963..11988)
                     /locus_tag="NBL02_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HCD2570871.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12197..13066)
                     /locus_tag="NBL02_000156"
     CDS             complement(12197..13066)
                     /locus_tag="NBL02_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HCD2570872.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13525..15417)
                     /locus_tag="NBL02_000157"
     CDS             complement(13525..15417)
                     /locus_tag="NBL02_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2570873.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15414..17141)
                     /locus_tag="NBL02_000158"
     CDS             complement(15414..17141)
                     /locus_tag="NBL02_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2570874.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17157..17807)
                     /locus_tag="NBL02_000159"
     CDS             complement(17157..17807)
                     /locus_tag="NBL02_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HCD2570875.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18011..18307
                     /locus_tag="NBL02_000160"
     CDS             18011..18307
                     /locus_tag="NBL02_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCD2570876.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18479..19099
                     /locus_tag="NBL02_000161"
     CDS             18479..19099
                     /locus_tag="NBL02_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCD2570877.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19191..20357)
                     /locus_tag="NBL02_000162"
     CDS             complement(19191..20357)
                     /locus_tag="NBL02_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCD2570878.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20369..20722)
                     /locus_tag="NBL02_000163"
     CDS             complement(20369..20722)
                     /locus_tag="NBL02_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCD2570879.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20850..21872
                     /locus_tag="NBL02_000164"
     CDS             20850..21872
                     /locus_tag="NBL02_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HCD2570880.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            21891..22052
                     /locus_tag="NBL02_000165"
     CDS             21891..22052
                     /locus_tag="NBL02_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2570881.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(22311..22745)
                     /locus_tag="NBL02_000166"
     CDS             complement(22311..22745)
                     /locus_tag="NBL02_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2570882.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22855..24714)
                     /locus_tag="NBL02_000167"
     CDS             complement(22855..24714)
                     /locus_tag="NBL02_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCD2570883.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(24832..25992)
                     /locus_tag="NBL02_000168"
     CDS             complement(24832..25992)
                     /locus_tag="NBL02_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HCD2570884.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26299..27723)
                     /gene="araA"
                     /locus_tag="NBL02_000169"
     CDS             complement(26299..27723)
                     /gene="araA"
                     /locus_tag="NBL02_000169"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCD2570885.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27739..28437)
                     /locus_tag="NBL02_000170"
     CDS             complement(27739..28437)
                     /locus_tag="NBL02_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCD2570886.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28394..30055)
                     /locus_tag="NBL02_000171"
     CDS             complement(28394..30055)
                     /locus_tag="NBL02_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HCD2570887.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30273..31673
                     /locus_tag="NBL02_000172"
     CDS             30273..31673
                     /locus_tag="NBL02_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HCD2570888.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31925..33004)
                     /locus_tag="NBL02_000173"
     CDS             complement(31925..33004)
                     /locus_tag="NBL02_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCD2570889.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33090..34049)
                     /locus_tag="NBL02_000174"
     CDS             complement(33090..34049)
                     /locus_tag="NBL02_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HCD2570890.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34061..35572)
                     /locus_tag="NBL02_000175"
     CDS             complement(34061..35572)
                     /locus_tag="NBL02_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HCD2570891.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35594..36157)
                     /locus_tag="NBL02_000176"
     CDS             complement(35594..36157)
                     /locus_tag="NBL02_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HCD2570892.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36163..37026)
                     /locus_tag="NBL02_000177"
     CDS             complement(36163..37026)
                     /locus_tag="NBL02_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCD2570893.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37040..37936)
                     /locus_tag="NBL02_000178"
     CDS             complement(37040..37936)
                     /locus_tag="NBL02_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCD2570894.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38111..39400)
                     /locus_tag="NBL02_000179"
     CDS             complement(38111..39400)
                     /locus_tag="NBL02_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCD2570895.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39372..40889)
                     /locus_tag="NBL02_000180"
     CDS             complement(39372..40889)
                     /locus_tag="NBL02_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HCD2570896.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            41087..41977
                     /locus_tag="NBL02_000181"
     CDS             41087..41977
                     /locus_tag="NBL02_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HCD2570897.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="NBL02_000182"
     CDS             complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="NBL02_000182"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCD2570898.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(42886..44358)
                     /locus_tag="NBL02_000183"
     CDS             complement(42886..44358)
                     /locus_tag="NBL02_000183"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCD2570899.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(44995..45357)
                     /locus_tag="NBL02_000184"
     CDS             complement(44995..45357)
                     /locus_tag="NBL02_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HCD2570900.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            45580..46332
                     /locus_tag="NBL02_000185"
     CDS             45580..46332
                     /locus_tag="NBL02_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCD2570901.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(46472..47395)
                     /locus_tag="NBL02_000186"
     CDS             complement(46472..47395)
                     /locus_tag="NBL02_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCD2570902.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(47410..47682)
                     /locus_tag="NBL02_000187"
     CDS             complement(47410..47682)
                     /locus_tag="NBL02_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2570903.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        13915 a         9942 c         8254 g        15702 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgca ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagccaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt tttttgatca taaacttgct tatacagaat tcgatgaact
     4501 gaatcataac tatgaagaga tgcaacgttt gattgaaacg ttgaaagaag atgagaatga
     4561 gaaagaattt gtggaaagtt tagagaaaag caacaaactg atcgaagcaa tcttacatgc
     4621 aaaaaacaca gggaatgtcc agcaaatggt cgaagcaact tatcattatg aaaaagatat
     4681 actggatcga ctgatttctg gtcaaagaca aggaattcca atcattgaac aacaaaaacg
     4741 tgtcgagttg ctccgttata tgaaagaaca tcagatccaa agatattcta tttttgactt
     4801 gccagcgcat ctccctttag caaattacta cgaaaatatt ttttcaggaa tgatttctag
     4861 ttttttgatt ttatgtataa ccgccttgtt tctttcttcc atcatctctt atgaaaaaag
     4921 aaagcaggta atctctctag tcaatctatt accggatagt atggtaaaga aacatagcat
     4981 ccgttttacc atttattatg gtgcagctat gctaagtctc gtgatgccat ttttgatcgt
     5041 ctctatacta gtaataatca aaaatggttt gggagacttc cgttatcctg taggaacgat
     5101 cattggtcag gagatacgaa tccttccgat gtatgagtat cttttccaga gctttctgtt
     5161 cctgttgttg tgggtgctct ttttaagtac aatcagtttt cttctatctg ctttatttga
     5221 acattctcta gtcaatcttt taggtacact tctttgtctt ttcttagcag agtatcgctt
     5281 gttttcttcg attggatgga tagaatcaat cagtcattac ttgccaactt cttatgttga
     5341 ttttcaaaat gtgatcatag gaggagatat tttctcgcca ctagcttcag aacaggttac
     5401 tttcatgaat ggtatcttga cacttggtat atggagtatc gttttattat tcataggtat
     5461 gggcactatc tatataaaaa agagctatta aaacacaaaa ataatagggg cggtgacaaa
     5521 aaccttgtca ccgtccccta tttcaaataa acggttttct agaataaata gttttcaaaa
     5581 gcttgcatcc gcagtaataa gaacctccca acaaaaatag ctcctcttat ttttatcttg
     5641 gttgaagtta ttacttgatg caaggcagct ttttttactg tgaacattgg ttatatatgt
     5701 ccatgttcaa aaatcatttg gacttcttca atggttttcg catcactgag cgccattact
     5761 tggtcgatgt ctgaacagaa aaaggctaaa tcttggagca ccgctaactg atgttctttt
     5821 tttaaagcca ctacaaataa gagtgtcgcg atttttggtt cctcttttgt tccaaagtcc
     5881 acaccatcag gcacttggac taaatagatt ccttcttcta ctacttcttc atctttgtca
     5941 gtcccatgtg gcaaggctac gaaattcccg atatagacac tcgctttttc ttgtctttcg
     6001 atcattgatt ggatatatgc cggtttgatt cccccttgtt gggtcatcca ctggccgatc
     6061 tcggtcaacg cttctttcca attttgatag gattgatcga gcaatatttt ttctgctggt
     6121 agctgtatca tcttttcacc ttcatttctt tatttcttca atcaggattt tcgctaacaa
     6181 ctgataaacg atttctttat ttcctgagcg gaagatctcg gtgttcaagt cattcatgat
     6241 gattgctcca ctgatctttc ctaaaagaat cccgtctttt tcttggattg gcgaaggcgc
     6301 cagcataact aacatacggg acaaatcgac tggctgtcta tccatccctt caatggtcaa
     6361 cggatgtttc aaattgaaaa tacaaaagat cggtttagaa atcaatgagc tggatgtatg
     6421 gaataacccc atatttgtat gaggaatacc gataggtgct tgttcatatc gcttaagcag
     6481 ccggcgatga accacctctt catccgttac tagtccttca ggaaaataag gaaataagct
     6541 gcgaatcgtt tcggaaatcg tctcaggatt atccagttct tggataaaaa aacgaccgag
     6601 taattgattg atttgatcca tgaaatgcat tgtttcctca taagtatctt ctatcatcca
     6661 ctcgttattg ctgttatacg taattttttg cctttttttc ttctcaatat gcgaaaaagc
     6721 ttcttttagc tgcttgactt catcttctag cagtagcggg gagactaaaa tatattttcc
     6781 accgtatcct ggtaaaatag atgtagaaat aacaacatca tattcttctt gtaaatcgat
     6841 tttttttaaa tcagcaatcc gatagaaatg aatttgttgg atgaagggaa aatacctttt
     6901 caatcgtagt tccaacatgc tagtagaagc taatccactt ggcgaaaatc ctgcaatact
     6961 gatattgctt tctttcggac ttcgttccaa tgaattcgca aaatgaagga ccatataagc
     7021 gatctcttct tcggataatt ttttttcttg aaaaatccgc tctactgctt tacggattgc
     7081 attcgcaact tcctcgtatt gaaccatgat tttttctaaa atcggattgg tcagtgtctc
     7141 ttcttgtaaa acggctcttg aaaacacacc agataaatga gtcaacagca ttttgtacaa
     7201 agtttcatct tcataaaaat ggacattcgt ctcttcactt acttcttgaa tcagttgttt
     7261 tactaaataa gcaagctgtg tatcgaacgt ctcttcaaag aaattctgat caaatgagtt
     7321 cgtgaagtca tttaataatc ctgcaaaaaa gacgatctca tttactggat aaagttgttt
     7381 tgtctcagaa gcgatccgtg aaaacaatcg tttagctgtc tgtagtaatt ctttgtttgc
     7441 cagactatta tatgtctcat ttgtcagtaa atgattttta gtcacacgct ccatcgtcaa
     7501 tgtcagcatc aaaaccaaat gttcaagctt tctatcactt agattggtct ttttttggat
     7561 caattcttcc ccgattactt tgcgtgcaag agatagatgt tgcggatcaa cgaatcgaaa
     7621 aaaataattc agtgatgcat cgaaagaaat actatgaaaa agttcatatt cattgatttc
     7681 taattctaat acattcgctg tcagaagccg gcggtatttt tccggtcctt cgatctcgta
     7741 tccctggttc cgaatgattt ccaaaggcag ttggcgaata ctttcttcca actgtttgat
     7801 atctgcataa aaagtggtat tactgatgcg atatgattcc atgaaatgag caagactcaa
     7861 cggctctgtc gtcagtaaca actcggctaa aatcgcgtgt tgtcgtttag cagtggataa
     7921 gtcattactt ttcccatctg tattttcttt taattgctct aatacttctt ctgatgcctc
     7981 aatgacaaat gagcctctag ctgctttttt caaagaagca tttaccgatt tcaatgagac
     8041 ttcaagatta tctaattcgc ggtaaatagt ccgtttactg accttcaata aattcatcat
     8101 tttttggata gatacaggtg acgggctact gagaaattct gctaataatt gtttttctct
     8161 gggcgataaa tacatcttat atttctccct tcacgagttt ttcgtattca ttcgtagcta
     8221 cccaatgttc aactagaact agccgaggga tcttttcttc tagctgttca gttacagcta
     8281 gttctctttg tgcgatataa atcgtttctt cttggatttt gattttttct attgattctt
     8341 ttgagataac aaactgtttt ttcgcttttt ttgctaattg acttaaaatc gtcatgccca
     8401 tagtttgtga gccgcgtctg ttgtttttat acaaaaatac aatattagca ggcgactttt
     8461 tttgttctgt gatatctgct atctcttttt tttcttcccg ctttggaaat tggatcgctt
     8521 cttttattcg tgacagcaag atatctgttt ccaaaaagtg atccagtaca tagatttgac
     8581 tttgaggtac tttttctctt gcaagacccg ctagttcatg ttgcacgata accagctgat
     8641 ttacttggtt attcaattca tagatagagc tgtatccgac agaaagatcg attttttctg
     8701 ctgctaattt ctttttcagc agggcggctc ccatagcact tgatcccata cctgagtcgc
     8761 aagcaaaaat gacggaatga atgatagatg gttccaagat ttctcccctt tctttgaccg
     8821 ttttgttctc tttttcttta tttggtaatt tcaatagtat cgctgcacaa atgaaggtca
     8881 caaatgtact gattgctatc cctatactta ccccagctag ttgtggataa ggcgcattgg
     8941 caaaaatcac gagtagtgac ccaggcgata caggtgcagc taatcccgta tctaaccatt
     9001 gaaatatcaa cgtgccgctt gccccaccag ccattacagc taaaaataga tacggtcgca
     9061 gcaagacaaa tggaaaatag atttcgtgaa tcccgccaat cgcatggatc atcaatgctc
     9121 cactcgcatt tactttttcc tgtttttctg caaaacaaag gatagaaagt aagatcccta
     9181 aaccaggtcc aggatttgcc tccatcaaaa ataaggaaga gtacccgact gtttgtgaga
     9241 attcgattcc taaaggtgtc aatacaccat gattgattgc attattgaaa aatagtactt
     9301 ttagaggttc taagatcaga ctgacaagtg gtaacagatt cattgtgact agccaagaca
     9361 caacggaggc gagcaacaag tgaacactat ttaaaactgg ttcaataaaa acgattccaa
     9421 ttatacaaat aacgctacca gtcagtccta ctagtaggtt gctgacaagc atttcatatc
     9481 cagccttgaa tttcacgtgg aatctagaaa caaattgttc atatacgatg ccggcaagcg
     9541 gcccgataat catcgctcct acgatttgtg gagcattgga acgagcgatc aaccctaaag
     9601 aagcaattgc accggcgact gccccttttt gtccccctac cgatttcccg cctgaataac
     9661 tgatcaagat gggtaaaagc agctggatca tccaatggca tacctcttct aatgtggtgt
     9721 gcaaatcgcc agacacacct tttgttgcta atgacaagat tccccaagcg ataaacagac
     9781 ttagatttgg catgatcatt tcatttaagt gatgtccagc ttttctcagt tgtttcatag
     9841 acacgcgact gctgttgttt gctttccatt tgtattccat ctttttcacc atacttctat
     9901 ttattgatgg acggatttta tgtagaaaaa gcagcaaacg aaaaaccgtc tgctgctttc
     9961 agctatctgg gaactatttg cctggttcag tcgcaatgat tgctagttca ccttggaccg
    10021 cccactcaga aacgccattt ggaataatac agcttgttcc tttttctaat ggatatgttt
    10081 ttccgtcaac gacaaggtct cctgctcctt cgattaccgt catcaatgta taaggggctt
    10141 gttttttgaa agatgtgatt cctttaatat cccactcata tacattgaaa aactcagttt
    10201 ctaaataagt gacgatcgat gaattccctt ttcggacttc tttgatttgt aattctggtg
    10261 ttttcgcagg gacagttgtt acatcgatcg attgttggat atgcagctct cttgtttgtc
    10321 cttgcgcatc ttttctgtcg tagtcataca cgcggtaagt cgtgtcagaa ctttgctgtg
    10381 tttctaaaat catgattcct ttaccaatag catggatcgt tccgcttggt acataaaaga
    10441 aatcaccttt tttaactggt actttcttca aaaggtcatc ccagcgccca tctttgatca
    10501 tctcagctag ttcttctctt gtctttgcat gatgaccata gatgatttct gcaccaggtt
    10561 cagcatcgat gatataccag cattcagttt ttcctaattc accttcatgt ttcatcccat
    10621 aggcatcatc tggatgtact tgtacagata gatcatcttc tgcgtctaag atcttgatca
    10681 ataatggaaa cactggttct gaaggatggc caaacaattc cttgtgttct gaccatagtt
    10741 catctaatct tttccctttg aattcgccat tttctactac actgacacca tgaggatgcg
    10801 cactgatcgc ccaatcttct ccgattttat cgttagggat atcaaaacca aagactgaac
    10861 gtaaacgact tcctccccaa attttttctt gaaaaactgg ttttaaaaac ataggttcct
    10921 gcataattca aactcctctc atttttcata gctattatag cattatttcg gtaataccgc
    10981 tttctctttc aaaaagtttt caacaatttc atctcgacat atgagatact ccgcacgatg
    11041 atcgacagga tgtacgcgat ttgacaaaaa tatcaacgct tcttgtgttt ttatatcaat
    11101 caagaaaaac gtacctgtat aaccagtatg aaaaagaatc gcatgaccgt cttctacatc
    11161 cttttttaag tcccatccta aagaacgata gccatttttt ttcggtgttt gatcttgcag
    11221 taaagaatca atcgtctctt cttctaaaaa acgaccatta cggatttttc ccttgttcaa
    11281 atacatttgg acaaaagtaa tgctgtcttt caaatcaata aagagacccg cgttacctgc
    11341 atgttcagcc aaaactcttg ctttcggatc atgagtttgt ccacgcaaaa cctcacccga
    11401 aggcagttgt tctgtgggaa cgattttttt ggaaggaaaa ggaagaaata agctgttcat
    11461 cattcctaat ggttccaata cttgttcttg aaagatttcc actgcagttt cttggtaaat
    11521 ttcctctagc atcaacccaa gaagaatcgt accggcatct gtatatttta cttcttttcc
    11581 tagttgctca cctggttcta gcttcaaata agcggcgatc aattcttcct gagatagctt
    11641 atctctgttt ggtatccatg ttttgatatc agaagtatgt gtcagtaaat gacgcaaagt
    11701 gattgtctga ttttcaaatt caggaagata tttatgaagc ggttgatcga gttctatctt
    11761 tccatcttct aatagacgta aaactaatga agtggttgca acgactttcg tcagtgaggc
    11821 tgcatcaaac aacagatctt ctgtcatcag ctgcttttca ggaacgactt gggcaaagcc
    11881 ggctgtaaat gtttctactt gattttcttt tatgaaggcg actgctacac ctggataaaa
    11941 tccttcttcc atctttttca aaataagctg tttcgtgtgt ggatacatct ttttctcctg
    12001 cttttctatc agattcttac agtccaagct taaagcttaa tctatgagga atcaaccttt
    12061 tttacacaaa aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt
    12121 tctatctcta gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc
    12181 gttaagaaag aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct
    12241 cactgacgaa ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt
    12301 tgtctaagaa gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt
    12361 cagtatcgat catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa
    12421 ttgcgcctcc gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga
    12481 tgccctcttc ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt
    12541 ttgttaagtt agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac
    12601 tgaatttttc ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc
    12661 tttggtcgtt cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga
    12721 gcaaaatccc catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac
    12781 ggatacgaca aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt
    12841 tcagcttttt gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca
    12901 tttcacttcc agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat
    12961 caaagccaac aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg
    13021 ctacactttt taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc
    13081 tctacgtcaa gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt
    13141 tgttaaaaaa aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa
    13201 aactcttata aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    13261 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
    13321 tgaacaccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagatac
    13381 aaaacggaaa aatagcttct catattttgc cgttttttga gctaataccg aaggacagct
    13441 gattgaacac cgtttattcg gaaaaaggga ctgtgacacg acttttgtca cagcccctaa
    13501 aaaaaataat aaatttgtac tattctatga agcttcttct tcagaaaact gactgttata
    13561 aagatctgca tagaatccgt ctttttccat caatgtatcg tggtttcctg tttctacgat
    13621 cgaaccatga ttcatcacaa taatgttgtc tgcatcgcgt attgtagaca agcggtgagc
    13681 aacaacgaaa cttgtccggt tttccaatag gcggttcatc gctttttgaa tcagtatttc
    13741 tgttcgtgta tctacactag atgttgcttc atcaaggatc aggacatctg gatttgctaa
    13801 gaatgcccgc gcaattgtga ttagctgacg ctgcccttgc gaaatattgc ttgcatcctc
    13861 gtttaataca gtgttatatc cttctggcaa tttacgaaca aaatcgtcta cgtgagcagc
    13921 tttagcagcc tcaatgattt gttcgtctgt tgcatcgtcg ttcccatatt tgatattgtc
    13981 atagatactt cctgtaaaca gccaagtatc ttgcagtacc attgaaaact gtgcgcgcaa
    14041 ttcatcacgt gaaagatcac gggtatctac accatcgtaa cgaatactac ctccagaaac
    14101 atcgtagaac cgttcaagaa ggttgatcag tgtcgtcttc cctgcaccag tcggacctac
    14161 aatagcgacc atctcaccag gtttgacatc caagttaaaa tcagtcatta gcaagttatc
    14221 tggtgtgtag ccgaattgaa cattttcaaa acgaactttg tatggtgtat ctttttcttc
    14281 tggaaggcca gatttttcat tggacatctc ttcttcatcc aacacttcaa atacccgttc
    14341 ggctgaagca actgttgatt ggatagtgtt catcaaactt gcaagttgag tgattggttg
    14401 agagaactgt gttgtatatt gcaagaacgc ttgaacatcc cctaatgtca tgttcccatt
    14461 tgcaactttt actccgccta gtacagcgac gaacacatag cctagatttt taacgaaatt
    14521 catcaacggc atgatgattg ccgaaataaa ttgagctttc caaccagcgg catacagatt
    14581 ctgattttct ttttcaaata cttcctggtc tttttcagta tgattgaatg ttttaacaat
    14641 ggtatgacca ccatatgttt cttctacttg gttattcaat aaacctaaac ttttttgctg
    14701 atctgcgaaa aacacttgtg atttcggcgc tacgatcatc acgatgatca agcttaaagg
    14761 gatcatcaat aacgcgacta acgtcaattg ccagctgatt gaaagcatca tcacgatgac
    14821 cccaatcaat gtgactgcac tagttacaaa ttgagtcaaa ttttgctgca gcgtgcttgc
    14881 gatgttatcc atatcattga ttgctcgaga catgatatcc ccatttgtat gtgtatcata
    14941 ataggaaatc ggtacacgat tcattttggc ttccaaatca tgacgtaatt cataaactgt
    15001 tcgttgggaa acacgtgtca taataaattg ctgtaaaaag ttgaatacag cagaaatcaa
    15061 atacatcacg attactgtcg caatgatctg cgcgatttta tcaaagtcta tcggaaattt
    15121 tgttacttgt tggccttgtt gcatctgttg agcgccagcc attacacctt tgaatatttc
    15181 tgtggttgct ttccccaaga cctttggtgt ttggatttgg aagatcacgg ctgctattgc
    15241 tagtacaaat acagcgatga tcgcaaccat acgtttagac atatagccaa acaatcggcg
    15301 taccgttccc cagaaatttt taggcttttc accttttaca ccgagatttc tgccaggtcc
    15361 accatgtcct ggaccgcctg ctggtccatg agagggacgt gtgtttgttt tactcatgct
    15421 aagtcctcct ctcttagttg tgaatgaacg atttcttgat aagtttcgtt tgttgctaat
    15481 agttcttcat gtgttccttt gccaaccagt tttccttcat ctagcacaag gatcatatct
    15541 gcatccatta ctgtacttac tcgttgagca actaaaacgg tgatactttc tttcatattc
    15601 gttttcaatg cttggcgaag attggcatcc gttttgaaat ccaacgctga gaaggaatca
    15661 tcaaacacat agatatctgc tgttttgact aatgcccgtg caattgccag acgctgtctt
    15721 tgtccaccag agaagtttcc tcctccttgt tccacatggc tatctaagcc atcttccatc
    15781 tctgaaacaa aatctttagc ttgtgcgatt tctaatgctt tccaaatcat ttcatctgtt
    15841 gcatccggct taccatactg catattgtct cggatcgttc cagagaacag aactgctttt
    15901 tgaggaacaa agcccattaa ttctcgcaaa ttatattgag tcatgtcact aatgtccgtt
    15961 ccatttattt ggatcgaacc agattcgatg tcatataatc gagggatcaa attgactaag
    16021 gtcgttttac ctgaaccagt cccccctatg attgcgacga tctcgcccga cttggcttgg
    16081 aaatcaatat cttccaatgc aagtttttct gctccatgat atcgataatt gacatgattg
    16141 aaggccaatg ttgcgttttt tccagcaaaa gaaactgttt taggattttc agggtcatgg
    16201 ataccgattt tttcatccaa cacttcatta atacggtcag ctgaagcttg tgcacgaggc
    16261 accatgataa aaatagcgga aagcatcata aagctgatca agatctgcat cgcataagtc
    16321 ataaatgcaa tcagattacc gacttccaaa gtcatatctg ctatgtagtg tccaccaaac
    16381 caagtgattg ctacgtttgt accgctcatg atcaatgtca tcattggcat catcaatgcg
    16441 acgatcgtat taactttgat tgccgtattt gtataatctt tattcgctaa atcaaaacga
    16501 ttttcttcaa agcgtgtctt atcgaatgct cgaatcactc tgacccctgt caatccttct
    16561 cgaaatacca ggtttaaacg gtcagttttc ttttgcatac ttttgaataa tggaacagca
    16621 aagtacatga ttccgccgat caaaataatc attactggga tgacaactaa gaagattttt
    16681 gtcaactgat gatctttttg ataagcaagt atgctcgcgc caatcaacgt gattggtgca
    16741 ttgatcatca tacgtaaaaa catttgtgta accatttgga tttgagtcac atcattggtc
    16801 gttcttgtga tcaaagaggc cgttccatat ttatcaaacg catcttttgt cagactctct
    16861 gattttttat agacatccgt acgcaattgc ttacccagtt tctgtgattc acgagtagca
    16921 aaaaatgtat ttgcgatagc agctaagata ctgatcaaag aaaagccgat catcacaaaa
    16981 cctgtatgcc aaatataatc gacatctcct tgcgctaccc ctttatcaat aatattggag
    17041 gtaagtgtcg gcaaatataa atctgccagt acttggatca ccatgaaaac tgcagctgca
    17101 attgctgaag tcaaggagat ccgttttacg atttttagca tctagttccc ttctttctat
    17161 ttatatactc cgtttttcag ccaagaaagt ttcaaattga attcactgat tgcttgctct
    17221 actgagtaat cttcagatgt tgaaaataag cgatacgaat gcgcaattgt tgtaaataca
    17281 gtattcatca ctaattggat cagtaattcc aattcatgat cattttttac tttcagcttc
    17341 gtccgatcga tcaattcaag caactctttt gcatcttgtt tgtgttcttt atgtggatcg
    17401 tgaggcgttt tttggcgact cgtatgagga gcgacttttc ttgaggcacg gtaatccatg
    17461 ttcataaaca agttacgata aaaagaagca ttctcgcctt ccaaaatctc ataaatcatt
    17521 ttagaaaaat aggattcgaa tcctttgaat agatcaccat cggattcttt cattagttgt
    17581 aacatatctt tagagctgtc acgtctcatt gtctcaaagt aataaaaata aagatcctct
    17641 ttatcttcaa aatactgata gaaactgcct ctagggatat ccgctaattt gacgatatta
    17701 gcaatcgaag cttcttttaa gggtactcta gaaaactcga ttctcgcagc ctcgattagt
    17761 cttttttgtt tttctttagg caaatggaaa aatgtctgtg tcggcatatg gatcctcctt
    17821 tctttcaaga tgacacattg tcatatgacg ctgtgtcatt ataacacctc ctacctgttt
    17881 tgcaagacat ttgcatagtt tttttcatta tcatccgata cattaaaaaa gtgataagag
    17941 gaatccttcc atttgaatta atgagagttg cccattaaaa tagccacata ctaaacaaag
    18001 gatgagataa atggcccaat caattagtgt ccaagacttc aataaattgc ctctcgatgc
    18061 tgacagcatc gttttagata caagaaatac cttagattac acaaaaaacc acttaagcca
    18121 ctcgctttct atccccgctg ctatcttgcc aagacaatta aaaaatctag atccaaagaa
    18181 actttattat attctaagct atacaggcag gcgatcagaa gcattagccg atcaacttac
    18241 tgctaatggt tttcgtgctg tttatatcat tggtgggatg gaagctcttc agtcgagtgt
    18301 tgcttgaata ttgcttgtta gattcaaaag aggctgtgac aaaaaatcgt gtcacagcct
    18361 cttttatacc ctgtcttttt ttagttattt attcttcaac cttatcaata tgacttgata
    18421 gccttttctt tttctcatag ttataacaat catagcaata aaaggagaag gtaagcatat
    18481 ggcatctgtt ttagtagtca aaggtcatcc cctaactgca gaagaatcgc gcacagttaa
    18541 ggcattgacg tcttttttaa caagttataa agaaaatcat ccagatgatg aagtgactgt
    18601 tttggaactt tatcgcgatg atattccgga aatcgatgaa gaattattat caggttggga
    18661 agcattacgt gctggtgcag aattcacttc cttatcagaa agtcaacaac aaaaaattgc
    18721 tcgtttcaat gaattgactg atcagttcct tgctgctgat aaaatcgtta tcgcaaacgc
    18781 cttatggaat ctaaatatcc caacaaaatt aaaagcttgg ttcgataccg tcaatgtagc
    18841 tggaaaaacg ttccgctata cagaaaacgg gccagaagga ttagtgacag gtaaaaaagc
    18901 actgcacatc caatctaatg gcggtgtata caacggacaa gactttgcct ctcaatatgt
    18961 aaaaggtatt ttaaactttg taggcatcga tcaagtagat cagctattca tcgaaggaat
    19021 cgattatgac cctgaccgtg cagatgagtt gatgcaaaat gcattagata aagctgctgc
    19081 tttaggtaaa tcattttaaa aattgagata aatgcataca aaagagacag aggaaaaaat
    19141 cttcaaagca gcatttgtac cttggatatt ttcccatctg tctctttttg ttattctttc
    19201 tgctttttca acggattttc ttcataaaga gaagagattg ttgtgtacca ttcaaaaaca
    19261 tgagtaatca ctactttagc tgctgcataa acaggaatcc ccaagatcac accgatgatt
    19321 ccgaacaatt tcccagaagt cagcagaact aataaaatcg ttactggatg gattgctaac
    19381 tgattcccta gtactaatgg agagatcaag cgtccttcta ttgtctgctc tactgcaaaa
    19441 acgatcagta ctttgatgat catgaatgga cctgctacaa tggctaaaaa gatagccgga
    19501 atcatcgcta aaaatgatcc taagtaaggg atcaggttca aaaaacccgc aataatcccc
    19561 aacgtgacag catactctaa accaatgacc gcaaaaccaa tcataaacat gattgccaca
    19621 gcaaaagcca cagttagttg tcctcttata taagaagaaa cttgactatt catttcactc
    19681 agcacattca acgtcggttt ttgcatgcgt gtcggtaaaa atttgacaaa ataaggtgct
    19741 aattgtttac cgtctttcaa tagataaaat aagataaacg gcatcgtcag cagagccact
    19801 aaaatagtcg ctaccgcgcc gacaaaactt ccaagactct gaactgtact cttagatata
    19861 tcttggatca tatcgcctgc cgaattcatg aacttatcac ctgctgtttc caactgttcg
    19921 cgaaattggt tgaacagcgg gtcccgcaaa atctcggtta atttattatc aatcgtatct
    19981 acatattgag ggaagttact gatgaagctc actgtttgtt cctgaatctt agggatgatc
    20041 actacgctcc cccatgcgat tagtgcaaca accaaaacaa acagcccaat gatactgtaa
    20101 agccttggta cttttcgctt ttcaaagtaa tcaactaccg gattcatcaa gtaatacaag
    20161 attcctgcta aaatgatagg cagacctacg attgctaaaa attgccagat tggtgcaaac
    20221 aaatatgaaa cttttgtaaa taaaaagacg atcagcaata ttaacaatac aacaagtaag
    20281 gctgtgacta cttgattatt taaaaaccag cgccaaaacc acgataccgg tctgttcttc
    20341 ttattttttc cttccataat tcctcctgtt agttataaaa aatttggctt cccaaaccaa
    20401 tagcaggcag tctatgcgga tgaagataga ttccatttgc aatttgtcct tcttctggac
    20461 aagcatcaaa aatcaatgat acgtgagcta ctgtctctaa attcccattg gccatcggac
    20521 cgacataatc gatcgtatat acctgatcat caaaagcaat tgttccgcct gctttcaaag
    20581 aaaaaggttc gtcttttgat acttcttgaa tcaccgaata ttttcgtaag gcagctgttg
    20641 ctgttttatc gaacaaaatc agcatgggtt cttccttgtt tattgcttct gtcccaattt
    20701 ctgtaatctt tgcctgaatc attgcactct ctcctttaat tgctttcaaa aacagtataa
    20761 cacattctca ttctttagcc gtgtggaata attatgtgat agaataaaaa cgaaaactat
    20821 ttcattacaa ttaggaggaa tcagctcata tgatcgaaaa gataatttta ggaacctata
    20881 cgcgtcgcga aagtaaagga atctatacga tcgaccttga tacagaaaaa gaagaattag
    20941 ccaacctttc attagctgtt gaagaaaata gccctaccta tgtagcaaaa agtaaagctg
    21001 gcaatcttta cacagttact agtgtggatg gattaggtgg tgccggtgcg tacgataagg
    21061 attttcattt tttgaatgcg gtaacagagt ctggcgctcc tttgtgttat gtagcggttg
    21121 atgaagcacg tcaattacta tacggggcga actatcataa aggtgaagta aatgtctacc
    21181 atatattagg agatggcgaa ctgaaagcag cagattcaat attccatcaa gaagcgacag
    21241 gtcctcataa aaaccaagat catgcgcatg tccactatac ggatctgact cctgaccaac
    21301 gattagtcgt atgtgatctt ggtacagatc gtgtgtatac ttatgatgtg tccgaaaacg
    21361 gcaaattgaa tcttgtaact accttcactg cagagccagg aacaggccct cgccatcttg
    21421 tctttcatcc aaatggacaa ttcgcttatt tatttggtga attagacagc acagtccgcg
    21481 tgctttctta taataaagaa acaggtgctt ttgaagaatt acagaaaata tcgaccttgc
    21541 cagaagaatt cgacggtgaa aatggagggg cagctatccg catttcaaat gatggcaagt
    21601 tcctttatgc ttctaaccga ggacataact cgattgctgt attcgcagtt tttgaagatg
    21661 gttcccacat tcaaaacaag caaatcatct caacagaagg ggattttccc cgcgatttcg
    21721 cactagatcc aagcaataat tttgtcgtgt gtgccaatca gaacaccgat aatctgacat
    21781 tatatcgccg tcatcctgaa acaggattac tggaattact gaaaaaagat gtctatgtac
    21841 cagaatgtgt ctgtgtttat tttgatcgtt agtaagaaaa ggagaatgaa atgaatcagc
    21901 aagacgtatt agacagacta cgggaagaac taaatattcc tttctttgat gggaaaatcg
    21961 aagataaaga gtatagcgaa gaggaatatc agaaactcaa agccgattta cagaactact
    22021 ttgaacaata tgtacagaat gtagaaaact aatcagcttt ccatactatc tgaaaagaac
    22081 atcaggacaa gacttttccg tctatcctga tgttcttttc tacaaataaa aaaccgccga
    22141 gatggttaga ctcgacggaa aaggagtatt tactcgattt tgagtaagta caaggttgtt
    22201 gttggttata ggtatgtcat tatcataaag gattttgatc actctatcat aaaggtaata
    22261 ttacatttat attagataac tctagttttt attgttatca aagatttctt ttattttttc
    22321 aacaacaaat tagtcatttt ttggatggca gctgtcaatt ggtctgtcgc ttggtcttgt
    22381 tttgcttcag atgtattgaa tagatcttca tctacccctg attcaagatc gatactttgg
    22441 atcatttctt cacagcctgc tacatatgaa agataagctt tttcaaaatt tttatgaatg
    22501 cccattgctt ttgctggtgg acgcaaagta ctgactttct taagcattgc ccgatatttt
    22561 tttgttcctt cttcaaataa caggatgatc tcttcaagag tcgatttgtc cagttcactt
    22621 gtttttcctt catcaattgc gtttctgatc acttcatatt tcggatgcat tccttctccg
    22681 attgcttctg tttccttgac gatttgatcg atggactgca cataatagcc aatattcggt
    22741 aacatgttcg ttttcctact ttctccattg ttgttattat ttattctact tgtttttatc
    22801 ctcaaatgac aagtacttga aaaaatttaa tggtttctat acagctcgcc attcttattc
    22861 gtgctcaaag aaaccatctg ctaaactata ttgtttccct gcgacaatgt catattggat
    22921 caattgatat tcgtcgtaaa tctcttgttg cgagcgattg aattgcgctt ctttatacca
    22981 ctgtccgttt tgatagtaca gttctcgttc aaacgccgga agttcctgtt ctaatgataa
    23041 aagcagctga taaaagccat ttgttgtctg attcgtctgt tccattagat tcgctgcaaa
    23101 ataaaacgga ctggtgattg catcgtgagt cgtttgcttt tctaattctc ctttactgtc
    23161 gaacataaga aattgtgttt catgcaatgt ttgtttgtca ttttttgctt ggatcgtatc
    23221 tgaatagatc cctggaagat gatcccccca aaaaacaact aaagtccttc tagaaagatt
    23281 cttcaattct tccgtgaact gcttcagtgc cgtgctgcta taggaaatat cttgcagata
    23341 attctctaag tcaagtgtcc cacttccatc tgaagttttt gcaggatagt ctagattatc
    23401 atactttcca tcgtagggca tatgtgtctg catcgtgact aaatgaatga actgaggtgt
    23461 tttttcttcc ttgagaagtg tcattacttc tttgtaagct gactcatcag aaatatatgg
    23521 attattctct atcgtatccg tataggtcat cgtacgctcg ctgatgaact ggtcaaatcc
    23581 taatgtctga taaacatcct ctcttttata catcgaagta ttgtatggat ggatcgctgt
    23641 cgtctggtat gattgtgcgt ttaacgctga aacgatagaa ggaagctgat ccatttttgg
    23701 tacaagcatc gtgtaagggg tggtgagttg cgcattaaat aacgccatag aaaaaccagt
    23761 caatgcttca aattcgatat ttgctgttcc accgccatag ttttgagaaa gcatattgcc
    23821 actatatgtt tgatcagcta cttcataata atctgccaat ggctcaccag atacttctac
    23881 gccgtttaat cgactaggat ctgagaaact ttcactcatc acgaacacga tgttcggcga
    23941 ctcttcctct acagttttat ttttttcatc ggctagcttc tggtattttt cagtgatttc
    24001 tttgatcttt tctttcgaat atccttcagg ttcatccatt ggttctacct tcagattgta
    24061 caaaaaccca ccgataaaac cagtgttata gtagttcatt ttctggctgt aaggtatcca
    24121 tagtgccgtt ttattatatg cttttcgcaa caaattatcg ggattgttga aattactgat
    24181 ataccctagc agtccaattg ccacaagcag tgtcgttaat cggatcaatt ggaatcgcct
    24241 tccttttttc aggcttcgaa acatgtacca gcaaaacaat cctatcacgg ttcctgcggc
    24301 agctaagatc actgtaaaca gcatagtgcc ggtcatatct ttcagcaatc cgatttcagt
    24361 gatcatcttc aaatcgtctg gataaatcgg ttcttgccga taactcattt tcatataatt
    24421 tgcaaaacct aatacaccga tggctacgac atacgtaagg gaacctaaag ggacagatcc
    24481 tgctagtgca atcagaaaaa taagaataat cagcaagacg agacaggcta agaagaattt
    24541 ttccgtatgc caagaaaaag caaatttcaa cgcaaggtct acggatagat cattttgaca
    24601 ccactgcaaa aataaattac taaaaacaac aagaaaaaag atactgatga ttgctgctgt
    24661 tccttttaaa atctttttct gtttggtcga aagtctgctt atatatcttt tcaaatgctt
    24721 gctccttcat ttctatcaat aaaagaatgt tgcttctctt attttagttg tacgaaattc
    24781 gaaagtaaag aacggaaaga agaattaatg aaatccaaat attctctttc tttatggcct
    24841 ttctggtttc tttaataagc ggacctttcg tactccagaa attttttcta gctgttgttt
    24901 cacttgattg agctgttcaa aatcagtact gtctatgtca atcaatatgt agacatgctc
    24961 attcttatgg ttacgcgata tatctgcgat cgctagctcg ttctcattca gcagagaaaa
    25021 tatctctgct aatatattgg ttttcttttg ataaaatata gtgaatcgaa acggcgcttg
    25081 gaaaaacatt cgtgcggatg gataattgac cgcttctcta accgtaccga acaaaagata
    25141 atcttttaag gcttcaactg ccaaacgtcc gctgtctgcc aaagcttcct gagtcgatcc
    25201 ccctagatga ggcaacataa gtatacggtc attttctaag aactcttctt ctggaaaatc
    25261 agtcaagtat ttagccagat gttgtttttc caatgcttgc ataacagcat atttatcaac
    25321 aatttcactt cttcctacat tgataagtac agcatctttt ttcatttttt ctatattcgc
    25381 ctgatcgatc aatccttttg tatcttctgt caacggcagc atgactacga tgtaatccga
    25441 ggtagaaaga actgtttcta aatctgcttg ccgaacataa tccaactggg catcgcggat
    25501 agaataaccc aagacatcca tccctaagct atagcaactt agcgctactt ttgtgcctat
    25561 cgctccaaga ccaagaagac cgatggtttt gccttgcaat tctcgtccca cataagcact
    25621 tcttttgttt tctgcctgtt ctaaaatatt aggacctgtt agtgtttgta ccatacgact
    25681 tgcttcgatt atcggacggg aagataagag caaacagcac aaaacaagct cttttactgc
    25741 attagcattt acacctggtg tattcatgac gatcgtcccg ttttcagaag ctttttcaac
    25801 gttgatcgtg ttcaccccaa caccagctct tgaaattgct agcaaatctg gtgtaagcca
    25861 ttcatctttc actttcgtac tacgaataac tattccttcg gctggcaaat catctattat
    25921 tttgaaatat tccccttgta acttttctat ctcttgttct ggaatagcat caatcaaatg
    25981 aacgtgatac ataaaacccc ctccttacta acaagtatct cgaaatttct gcaattagac
    26041 aactgtttac catttgactt cctagaagca aaaagagcag ttagagcaga gtaggctgag
    26101 tgataaaaat aaggctatga caagttactg ccataagcct tattttcgag agcaaaatat
    26161 tgagggtgag atatcttgat aaatggtgtt ccgttagctg acattgataa taagactaaa
    26221 tttcaaaaaa gttgaaaagc aaatttcgtc aatctcttct tatttcttaa aactaagtgg
    26281 ctttttcaca acctcttttt atctaataac cactgtttcc aactcaacta attttgccca
    26341 cgtttcaatt tgatctgttg ttagatttag cgagacaacg gtatgatgac cgccacctgc
    26401 ctgaatccat gaatggacac cttcatggaa attcggttta gttttccata acacacgtgc
    26461 aacaggtaaa ttaggtgctt cagtcgttgg ttcaaaggct tctacttcat tgatcagcag
    26521 tttataatgc gtaccgaaat ctgccatcga tacgactacc ccttctcctg ctttcccatc
    26581 aaatacaaga cgcgccggat cttctctatc tcccattgat aaaggagaaa caacgattct
    26641 tggtttcgtc gcagcaagcg tcggatctac ttccatcata tgggattcca agattgcttc
    26701 ttggccacta gctagctcgt aagtataatc ttccatgaat cctgtatctt tccctcgagc
    26761 catgattttc agcaaccgat caatcgctgc agttttccag tcgccttccc cagcaaaacc
    26821 gtatccttct gccatcaaac gttgtacagc tagtccaggt agttgtttca ttccatacag
    26881 gtcttcaaaa ttagtcgtaa aagcgttata gcctccttct tctaagaaac ggcgaatacc
    26941 gatttcctgc tgagcttgta cttttacatg ttcctcccat gtcttttctt cataatcacc
    27001 atagtcgaag gtatacaatt ccttgtactc ttcaaataat tgatcgactt cttcgtctga
    27061 tacgcgatcg attacttgta caagatctcc gataccaaaa taatcaacag tccaaccaaa
    27121 ttgaatctgt gcttccactt tgtctccttc tgtgacaccc acgtttctca tgttgtcacc
    27181 aaaacgagcc acttttattc caaaactttc attataggct actgcaacat ccatccaatc
    27241 agcaatctct ttttggatct caggtcgttt ccagtagcca acgacgattt tattttgttt
    27301 atttaatcga gcattgatga acccatattc gcggtcacca tgagcggatt gatttaaatt
    27361 catgaaatcc atatcgatcg ttttccaagg aatgctttcg ttatattgcg tagctaaatg
    27421 aagcaatggt ttttgtagaa gctttgtccc gcggatccac atttttgctg gtgagaaagt
    27481 atgcatccaa gtgatcacac ctgctacttc ttctcgataa ttgacctctt tcatgatttt
    27541 tgtgatcgta tcagcagtaa ctgctaattc ttgaaggcgg attggataag gcaattggcc
    27601 attttcattc agttcatcga ccatttcttg tgcatgtttc tttacttctc gcagtgcttc
    27661 ttccccatat aaatgctgtg atcctacaac aaaccaaaat tctttttctc caatattcaa
    27721 cataattagc cactcctttt atttgttttg tccgtaatac gcattttttc cgtgttttcg
    27781 taaataatgt ttatctaaaa tccgttgagg cagctcttct gaaaatgaat tgagctgtct
    27841 ggtaaataaa ttcatcttgc atacttcatc tagtacgact gcgttcatta ccgcactttg
    27901 cgcatctttc ccccaagtaa atggaccgtg tccgtgtaaa agtacccctg gaattgctaa
    27961 gggatcaatt ttgcgttctt tgaatgtttc tatgataact tttcctgttt catattcgta
    28021 cccgctgtcg atttcctgtt gtgtcaaaaa tcgagcacaa ggtaccgatc cataaaaagt
    28081 atctgcatgc gtcgtcccca tcgcaggaac atccaatcct gcttgtgccc agatcgtcgc
    28141 ccatgtcgag tgagtatgga cgattcctcc gatttgagga aatgaacgat ataaaacagc
    28201 atgagtaggc gtatccgaag agggattgag tttaccttca atgatatgat tgtccaagtc
    28261 cacaacgacc atgtcgtctg ctgtcatctc ttcataactg actccgctag gtttgataac
    28321 gaaatatcgt gtttcaggat caaaagcgct cacatttccc catgtatatt tgacgagtcc
    28381 gtgtttaggc aagtcaagat ttgcttggta tacttcttct ttcagttgtt ccagcatttt
    28441 ctatcactcc tttttcttgc aattgatgaa tcgctgtttt ttcaatgtcg atcccctcac
    28501 ggtaacgtct cataaatatc tgatagcctg ctatttcttc tgctgaaggt gtgatggtga
    28561 ttccttggtc ttgtccaaac acgtcttctg ccaagaagtt ttctaaagac ttcccatgtc
    28621 gatccatcat ataggctgct aataacgcga ttccccacgc tccaccttct ccggctgtgt
    28681 ccatcactgt aactggtgcc tccatagcag atgctagaat ccgctgtcca acttctggtg
    28741 ttttaaaaat ccccccatgc cccactaaac gatcaatttg gacattctca gatttcaata
    28801 tatccattcc gattctcatt gcaccaaaag cactggctaa atggatccgc atgaaattcg
    28861 ctaaatcaaa acggctacct ggtgtacgta caaataatgg ccttccttca tttactcctg
    28921 taatgttttc accggaaaaa taaccataag aaagtaatcc cccgcaatcc gtatcgcctt
    28981 ctagcgcttt gttgaataat accgtgaata atttatctct gcttatctcc atacccaaag
    29041 aattggtaaa ttcttcaaaa atactgaccc aagcattgat atccgaagag cagttattcg
    29101 tatgaaccat gcctactagt tctcctgaag gtgtagtgac catgtcgatt tccggatgga
    29161 cattttctag ttctttttct aatacgatca tagcgaaggc ggaagtacca gccgaaatgt
    29221 tccctgtccg tttggctaca ctgttcgtag cgaccatacc ggttcctgca tccccttctg
    29281 gcggacaaac cgggattcct ggtctcaaat ttcccgtagg gtcgatcaga taagcccctg
    29341 attcgcttaa cttgccagct tcttctcctg ctactagaac tttaggcaaa atgtgatcca
    29401 agtcaatcgc tacgtgcgct ttttcgatta actgaccaaa aatatttttc atttcttgat
    29461 ggtaggtttt cgttgaagga tcgataggaa acatgcctga tgcatctcct acgcctagta
    29521 ctttctcacc agtaagctgc caatggacat aacctgcaag cgttgtcaaa aaattaattt
    29581 ctttcacatg ttcttcttta ttcaaaatag attgatacaa atgagcgata ctccatctct
    29641 gcggaatatt gtattgaaat ttttccgtta atattcgttc tgcttcttca gtgattgtat
    29701 ttcgccaagt acgaaatggt actagtaact gttccttgct atcaaatgct agataaccgt
    29761 gcatcattgc actgaaccca atcgacccga ttgtcgtaag attctcgcca tagatttcga
    29821 atacttctgc tgccattttt cggtaactga cgcgcagtcc tttccaaata tcttctaatg
    29881 aataggtcca gataccattt tctagtttgt tttcccactc ataactacca gcggcaatag
    29941 gagtaaaaga tgaatcaatc aatacagctt tgatcctagt tgatccaaac tcaatcccta
    30001 aagatgtacg accatctatg acatctaaca tactttgcgc atttacttcc ttcataatga
    30061 tttaactccc tccttctcgt caaacctgat caaaccgtct ttttagtagc gcttacaatg
    30121 attattgtat acttttgtac gtacatgtca agcggttatt tacaagaata caataaaaaa
    30181 cacttgtttt gtacgtacat aagttataaa atgaaattgc ttacaaaatc tgatcaaccg
    30241 ccttctcgaa attctagaag gagagaatac ttatgaaaaa agaaaaaaaa gtttcctcat
    30301 cgtttattta tttttttggc gcatttggcg gcattctatt tggatatgat attggtgtta
    30361 tgaccggcgc cttacctttt ttacagcatg attggggttt ggcaggtaaa gcttctttga
    30421 taggatggat cacgtcttcg gtcatgttag gtgcgatcct tggcggctct ttatctggtc
    30481 ttctttctga taagcttgga cgacgaaaaa tgattcttct ttctgcactt atttttatgg
    30541 ctggttctgt cctttctgca tctgcccctc acaatggttc ttattttctg attgcagcaa
    30601 ggattcttct aggtttagct gttggcgctg catctgccct agttcctgct tatatgtctg
    30661 aaatggctcc tgctagacta aggggaagat tgtcagggat caatcaagtg atgattgctt
    30721 ctggcatgct cctttcctat gttgcagatt atttgctaaa agtgttgcct gaaacgatgg
    30781 cttggcgcgt gatgttaggg ctcgctgctg tccccgcgct gattttattt ttcggtgttt
    30841 tagctctacc agagtcaccg cgttttctta tgcaatctgg ccgcttggaa gaggcaaaaa
    30901 gagtattgaa ctatattcgg acaccaaaag aagctgaaca ggaattcgaa caaatccagc
    30961 tgaatgtcaa acaagaaaag acaaccggaa cttcttggca tactttgttt ttagaaaaat
    31021 atcgttcctt agtcattgcc ggtattggtg tagcagtatt ccagcaattt caaggagcta
    31081 atgctatttt ttactatatt ccgctgatcg tagaaaaagc gactgggcat gcagcgagcg
    31141 atgcattgat gtggccgatc atccaaggaa tcattttagt tgctggttct ttgctctttt
    31201 tggttattgc agacaagttc aatcggcgaa ctttattgaa aataggcggt agcgtgatgg
    31261 gactttcatt catcctacca gctgttttag gaactgtact ggatgctcat acaaattcgt
    31321 tgttgattct cttgttttta tgtatttatg tcgcatttta ttcttgcaca tgggctccac
    31381 tgacatgggt aatcataggc gaaatattcc cactagctgt ccgcgggcga gcatctggcc
    31441 tagcctcttc ttttaattgg attggttctt tcctagttgg cttgcttttt cctgtgatga
    31501 ctgcttctat gtctcaagaa atcgttttcg gcatttttgg gatcatctgc tttttagggg
    31561 ttttatttat tcaagagatc gtaccagaaa ctcgtgggaa atctttagag gaaatcgaac
    31621 aaagtgcatc taaaaaaact tatcctaaac gtatttctat ccatcattct taaagaggtt
    31681 gtgacagaag tggtcagctt caagaaataa gaaggaaccg cttagtttcc agtcacaagg
    31741 aacaatttca aaaaacagct tctcatgttt gatgaatatt gtgacttgtg accagttggt
    31801 aggagaacac cgtttagtgg agaggtggtg aaagaaacgt tcagccttga gcgatgacaa
    31861 aagctctcac aaacattgcg acgagtaacc gtaggagcaa taagaggcgg taaaaacccg
    31921 tctcttattt ttcgttttct ttcactgttt cccgttcaac tagtacaggt tcatagagaa
    31981 tatttggctg tgcctgtcct ttttcaatcg ttgtgacgat ccaccgtgcg gcatcttgtc
    32041 ccaacttttc ttttggatgc gtcaatgttg tcaatttttt attccctaaa tggcttaaag
    32101 aagaatcgtc tgttcctacg atcgataggt catctggaat atgatagcca ttttctgtca
    32161 acttatctag cattaaacta gctatttgat cgttatagca aatgatgcca tctaaagctt
    32221 gttcttcttt caaccgttgt actgtttttt cagcgattgt ttctcgtgtt tccgtggtga
    32281 aagtaatgat atcttccgtc aaaaaacgca cattatactg ttcacacgcg ctgataaaac
    32341 ctttcatcct gtattttcct tgaagatcgt cgatttttgt gatgaacaac agacgctgat
    32401 gtcctttttt tatcaaatat tctgctgcca taaaaccgac tttcacatca tctaaacaaa
    32461 tataagaggc atttaactct tcataagcag cattgatcat caccatcgga atgccttgtt
    32521 ctctcaaagc aacataaagt gctaagtttg ggttatactg attacttttc gtaggctcaa
    32581 cgatcaaacc atctacccca taagcaatca ttttcttcaa acactctttc tccaattcgt
    32641 gatcgttatt cgtactggct aaaagcaatg aatacccaga ttctcgtaat tcattttcga
    32701 ttcctcggat aatcgacgga aaaatataat cagagagata tgtggtgatg acaccgatcg
    32761 tttttgttct gttcaatggt gttttttctt tcttatagct gtcatctaca tatgtgcccg
    32821 accctttttc tttacgcaga tagccctcat ttaccaacac agcgattgct tgccgaatcg
    32881 tatgtctgct gacgtcatat atttcttgca gcttgaattc tgagggaatg gttgtattta
    32941 cttcgtattc ctcacgaagg atcttttgtt ttatctcttc tgcaatatct tgatattttg
    33001 ccatttgcct tgcccctttt tatttgtacg tacatatatc ataacgagtc tgcaaggttt
    33061 attcaagcaa aaatcaaggt ttcttgtgct cataaggcat acggaacagg ttcaccaaat
    33121 tctggaaatc cttgctcatt ccacgtaaag cattgcgcgt tcgcgtgccg attaggattg
    33181 tctaacggat cccctttttg gttcttctct ggtcgagcat gatagatcaa aatatcaaag
    33241 cacccgtctt ctgaaaccgt aaacgagttg tgaccaggac caaattgctt atttttttca
    33301 gaagaaacga atactggctc cgataattta tgccatgatt cttttaataa aagattcttg
    33361 tgctcatcag cccacagcag gcccatggca tagttttcat ctgttgcact accagaatac
    33421 gtaatgaata ttttcccatt gcgagtaagg actgctggac cttcatttac tgaaaaaccc
    33481 cgtttttccc attcatactc aggtatagaa aggaggatct gcttcccctt taacgtccaa
    33541 ggattatcca tttcggatag ataaagatta gaattcccag taatttcagg atcttgttgg
    33601 gcccacacat aatatagctt tccttgagaa gagaaagttg ttccgtctaa actgaaagtt
    33661 tcccttttcg tttttatttg tcccttttct atccaatctg tattcattgg atctatatgc
    33721 tgattttcaa gtacgaacat gcggtgatga tgagattcat cacgtatagt tttatgattg
    33781 cttgcagcaa agtaaatata ccattttcca ttgatataat ggacttcagg tgcccagatc
    33841 aattcactca tcggtccttc ttcatgagcc tgccatacaa tcaggcttgc agcatgttct
    33901 aattcattca gtgactttgc ccttcttaat tcaatcactt ggtagcctgg aaccgatcct
    33961 gtaaaataat aataaccatc tgtatgttta tatacccaag gatctgcccg ttgccgaacg
    34021 ataggattat tataaagtgt cttcatcata acaaatttcc tcacattttc tttattctaa
    34081 ttacattcca agagtatttt tctatcccta catttaattc gtcatgtttt tctattgaaa
    34141 aatagtttat ttcaggtttt attttttctg gttcttcacg tgtatttttg tccaacagat
    34201 cgctactggt taatgtgtag tgctctcctt gatctagcaa attgaaatca tgttcaatcg
    34261 ttatcttttg ttgttcattt gtgcgattga tcaagaaaat agtcagctga ttgccgttat
    34321 ctacgatcac actgtccaca taaggaacat tttttatctt ttcacaatca taaacagggc
    34381 aaatgatttg ttgtttcaat acttgacctt tagcatattt cgataagtgt tgcatcacat
    34441 aataagttgg attcctccat gcttgtcctt tagcatctgt cagtattaaa ggtattgtgt
    34501 ttactaacaa tgattgacaa ctaatcttta cgcgatctgc gtgtcgtaaa atagccagcc
    34561 ctaatgaacc aaataataat gtatcttcca tagtaaagta acaccaatcg atgggactac
    34621 cgacttcaaa atccctatgt cttttttccg gttgggcatg tacattccat tcatcaaacg
    34681 ctagatacat tgttttctca cttctcaaat atccttttac ataatcacaa gtagcaacga
    34741 tttcttcaat ttgacgatcg aatcgataag cttttgcaag gtatgtcgct gtatcatcac
    34801 gctcattttt aggaggttta gtcaagtctt cttcatcata cttatcaata tagttatgca
    34861 aagctaaata atctacattc tcataagctt cttctaatgc gatacgatcc cattcagggt
    34921 acgatggtaa acgtggcgta gagcttccaa ctaagattgt ttcaattgaa tcatccacta
    34981 acttcatgac ttttgaggct tcatttgcta acctaccata ttcataagca ctcttagccc
    35041 ccacttgcca tggaccgtct agctcattcc ctaaacacca taatttaacg ccaaaaggct
    35101 ttttttgccc attttttatt cttaaatcac tccagaaggt tccttttgga aaattacaat
    35161 attctactaa attttttgct tcatcaattc cttttgttcc taaattcacc gtgaatatcg
    35221 gtttcgcttg tacatgactg atccatttaa aaaattcgtg gagtccaaaa gaatttggtt
    35281 ctatacctcg ccatgctaaa tcaatttttt ttggtcgttg ttctttccta cctacagtat
    35341 ccacccaatc ataaccagat gtaaagtttc ctccaggata gcgaatgaca cctaattcta
    35401 attcctttac ataagagagg acatcttgtc taaacccgtc accatcagct gtttcatggc
    35461 ccggttcata aatccctgta tagacgacac ttcccatatg ctcaacaaat gagccaaaca
    35521 gccgagaact gatctctcct accgtaaaat cctctctgat ttttactttc acttcttcgt
    35581 ccaacagctt tccctacctt tcatttccta tagtcaagat aatagataca agtttccata
    35641 cgccaccagc aaagataaaa ataaaaggag ggaaaagaaa tatgagcagt acaaatccta
    35701 ataacaatac gcaacaaaga atatttctca aaaaatcaaa aaatactttc catgcacttt
    35761 gttcaaaaat ttgtaaaaat gtgatatctt tgttttcaga aatcacatat atccaggata
    35821 aaagaaacat ggaataaata agtaaaacta catatccact aattgaaagt acgattgtct
    35881 gttcttctaa taattgatta acaagaaaat aaataatgat cgttaacaga ctaaggaaca
    35941 agttcttacc aaaatgttgc tttattcctt gaaagaaaaa ataaatgata tttcctgatc
    36001 cctcaccttt ccattgcgcc attacataaa aaacagcaca tgtagcaggc acgatagtaa
    36061 taacaggcaa acacacgatt acccagagaa tacctaataa gatgtaatca gaaaccacaa
    36121 tcaactgtcc ataaaattta gtctcaacta ttttcatctt cctcactctt tcattccaga
    36181 catagctgtt ccttgaacaa aatatttctg tgcaaaaata taaaccacta gtaatggcag
    36241 cattgaaata accgttccag ccattacagg tccataaaaa gaaatgttct gccctgtaaa
    36301 taaagctaac cctgctggta aagttctcat ttttgaacta cttgtcaata tcaatggata
    36361 aagcaagtca ttccaactat tcatcagcgt gaaaatccct aatgataaaa gagtaggttt
    36421 gctcaatggc aacatgattt tcaaaaaaat attaaactca cttaatccat caatgcgtgc
    36481 tgcctcttcc aaagaatctg gtaaagtaat aaaaaaggaa cgcatcatga agataccaaa
    36541 tgcagttgtc atacgaggaa tgatcaaacc tgcataagta tcaagcatgc caagcaaatt
    36601 gacttcaata aatagaggaa tcatgaagac ttggaatggg atcatcattg tcagcaaaac
    36661 gaaatagaac aaaactgatt ttcctttgaa acgcatacga gcaaatgcat accccgcaag
    36721 agaatcaaag aatacagaag tgatcactgt ccctccagca aaaatcactg tatttttgat
    36781 gtaatctaat aaagggatcg ttgtccaaac cttaatatag ttatctaggg tatagctatc
    36841 tgcaaatatt ctaggcggaa atgagataat gtctttttcg tgtttaaaag aagaaataat
    36901 cagccacaaa aaaggaaacg caataaccaa caatataact agcatactaa tcgttcgaat
    36961 gattttttta ccactaactg cttttcgact attcacagta actgtaatcg agctttctgg
    37021 tgccatacag gtttccctcc tatttcattt gtttttcttt ccgtgacatg tacacattag
    37081 tggagatagc tatagttgca acaatgataa acaaaaccga agacagtgct gatgcatagc
    37141 ccaattcata aggtgctgta aatcctcgac tgtaaatata ttgaacagct gtttctgtct
    37201 taaattgtgg gccaccagcg gttgcaacat atacttggtc aaacacttgc atcgaaccaa
    37261 tcagattcgt taaaatacaa aaacctaagg agggaacgat ctgaggtagc gtaatgttaa
    37321 aaaactgctg tcgatttgtt gcaccatcca tttgagcaga ttcatacaaa gagggagaga
    37381 ttccctgaat agcagtcaag agaatcgtca tagtaacacc aaaattcttc caaacagtca
    37441 tgatggctac cgtcggcata gcaagggttg gatcgcgaaa aaattgaggg atcggcaagc
    37501 cgacttgtga aaacaagtaa ggaatcgccc ctacattcgg atcaagcaac atagaaaaaa
    37561 taatcgcaat tgacgtcaat gaacaaataa ctggaatgta aaatgtcgaa cgacaaaaac
    37621 gattgaatct ggtattttta gataatgcta ctgctgctaa taaaccaacg ataatttgtg
    37681 ttggcgtttc gagtacggtg aaatataaag aatgaatcaa cgaattaact gctcgttcat
    37741 cactaaatat acgtgtgaaa ttttcaaaac ttacaaaatt tcttgatgta aagaaaatat
    37801 tcaaatctgt aaaactaata gcaaatgttc ctaaaagcgg tatgacagta aataataata
    37861 aaactaaaaa agatggagtt aaaaacaaat acgcagcacg atcaggacga ttccaatatt
    37921 tggtgaatcc gttcatatag tccacctcat ctttcatttt gataaaatat acaatcttgg
    37981 tttaaacgaa tctgaataag atatccgaac aatcgctgtg atttttgcaa taacacctat
    38041 ctgcaaatac taggtcgatc attcagatat ctgtatcttg ccaacagtct atcctgtata
    38101 aagttctgta ctatttttgc agtaattgat tgatggcatc atctgcatcg ttcatgagtg
    38161 tctcaggatc ttctcctgct aataattttt caatcagtgg attgatgata tcgttattga
    38221 tcgcagagat ttgggtaaaa cctggtaaat atggctctgc atagtccatt tgtttcgata
    38281 attcagatac aataggatca ttttttactt cttcatcttt tgctactgaa tccaagtagg
    38341 caggaaaacc attttcgata ctccattttt taccaatttc agttgtattc caatatttca
    38401 aaaactcata gacagcttct tttttattat tatctgtact tgccggaatg gcatatccta
    38461 cgccatctaa aatagctgtt tttttgtctg aatcactccc ttgtggtaag gtagtcacat
    38521 tataatcaat ctcgtttgct ttcaatccgt tatttaacca tggcccattg atttcaatgg
    38581 ctagttgacc agcattcatt aaattgtcca tttcggcgcc agaaatcgat tctggaccag
    38641 tcttgtctac atggatcatt ttttggatcg cttttaacgt tttaaggttc tcaggagacg
    38701 caaattcaga cttagtataa tcatcgttca ctaatttacc gccatttgct aacatccagt
    38761 tatataacgg tgcagttcca tccttattga agacaaaacc tgatacattt ttactactat
    38821 cagttaattt tggtgccatt tctgtcaatt cttcccaagt agtaggtggt tgttcagtat
    38881 ctaatccagc ttgtttaaac aaatcattat tccaatacat atacatgcct tgtacctgca
    38941 tggggacaaa atactgttta ccttctactt cacctaattc aatagccgtt tctttaaaat
    39001 cagatttatc tactccatca tattcccaga aatcgtctaa tggttgtacc gctccgtttt
    39061 ctacatactg tgcaaaatct ggataattta atgcaataaa gtcaggtgct tttttagaag
    39121 aaatcgctgt aggaagtttt tcattcaaat tggcccaagt catgatatcc atttcaattt
    39181 tgatgttttt atcatttgtt ttattaaatt cattaacgat ttcttgcaat atttcaccat
    39241 cagatgcagt aaacccattc caaaaagtca aagtcacttc tttatcagaa tcagattctg
    39301 ctgaacttcc gccaccgcaa gctcctaaaa tacctgttgc agctaccaac aaagagccaa
    39361 ttaaccattt tttacctttc attttttgtt cctccttcat ctgataaacg acatctgacc
    39421 acattccaag atagcggttt caataaacaa ttgattttcc catcttcagc ttttacattt
    39481 gttgaatgac ttggttttac ttgttcactt ttaggaagat tagttgcttt tgtatcaaat
    39541 ccactcattt cagaaaaatc aatcactccc tctaatgata attcagacaa gtgaacagtt
    39601 agattcattt cttcttctcc acggttaact gcaaatatga caagttcttt gtcatttatt
    39661 acggctatac tttctaaata cggcacttct tcaaaatctt ccgtcgcata gtttgctgaa
    39721 cgaatttgtg ggcttaacac ctctccttgt ccatacacag cagcttgcat aaatggataa
    39781 tagatcgtct gtctccaagc ttctccgttt gtttctgtcc tgattggtgc aataacgttt
    39841 accaattgag caagacaagc aatctttact ctatctgcat gtttcaataa agtgattaat
    39901 agacagccta atagtaatgc atcttcaaaa ttatagttat cttctagtaa aggaggagca
    39961 atctgccaag gcgctgcttg ctcatcttgt tccgctgaat gataccaaat attccattca
    40021 tcaaaggaca ggttgatctg cttcttacta tgtttcttcg ccttgactgt atcgcacatc
    40081 gctgtcactt cagaaatgaa ccgatccatg tctaaggaac gtgcaagata atgagaaaga
    40141 tcattttttt ggttgccata atattgatgc agagacaaat aatctatttg atcgtacgct
    40201 tgatcaagaa ctgttaactc ccaatcacca aatgtaggca tttgccgatt cgaacttccg
    40261 cataaaacca cctcaattga atcatctacg agtttcatcg ccttagccgt ttcctctgct
    40321 aaccgtccat attcttccgc tgttttatgt ccaatctgcc aaggaccgtc catttcattc
    40381 cctaaacacc atgtcttaat tccaaaaggg ctctcttgac cattcgaacg ccttaaatca
    40441 ctccaataag ttcctttagg aaaattacaa tattcgacta aattacgagc tgcatctact
    40501 ccacgagtac ccagattgac agccatattg atttcggccc ccacatgctg acaccagtta
    40561 taaaattcat gaatcccaac ttcattcgtt tcaattgtcc gccaagctaa atccaacctt
    40621 ctaggtctat ttgctatggg cccaatactg tcttcccaac gaaatcctga cacaaaattt
    40681 ccaccaggat atctaatcat gggaatgttc aattctttta ccaagtgaat gacgtcttgg
    40741 cgaaatccac ttttattagc tgatggatga tctggctgat aaatcccttc atatactgct
    40801 cgtcccatat gttcaataaa tgaactgtac aaccgcggat caactttgcc tacacgcttg
    40861 acactactaa tcaacattgt ggcttccata actgacctcc tttaatttat acatgaataa
    40921 tacacctcgt tgaaagcgtt gtcaataata taaatgttaa acttacttat tttttatcga
    40981 ttttaattaa ccaaaatatt aataatgatt aaaacaaata atttaatata acttttatta
    41041 acaaatatgt taatatataa gaaaaacctt ttaagaggtg atatatatgc aactacaaat
    41101 aaacgaagaa tccttaccgg tatatgaagc attagctagt aaaacaagga tcaagatcat
    41161 ccagctactt tccaaaaaaa agatgaatgt caaagatttg gcaaaagaac ttggagtgag
    41221 cagtgctatc acgacgatgc acgtgaaaaa gctagaggaa gcaaacatca tcaaaacaga
    41281 aaaagtagga caacaaaaaa tttcgagcct tcgtgtcgat aaaatcgata ttagttttcc
    41341 agaaaaaata ttcaatgctt ttgatacgaa agaaacatcg atacctattg gtcactatac
    41401 aaattatgca attgaaccaa catgcggttt agctacgatc catgatttta tcgggaaagt
    41461 ggatgaacca cgctatttta tggatcctcg aaggatggat gcccgcattc tctggtttac
    41521 cagcggattt gtcgagtatc aggcacctaa ttttttaaat acagaggata cattggagat
    41581 gttggaagtt tctgtggaaa tttcttctga atttcctttt tcaaatgaca attggccttc
    41641 agatatcact ttcagcctaa atggtgtgga gctaggaaca tggacgagcc ctggagattt
    41701 tgctgatatc cgcggtaaat atacgcctga ttggtatcca gataatctta atcaatacgg
    41761 cttgctgaag acgatccgaa ttaccaaaca cttgacgaat atgaatggag aacccttgtc
    41821 aaacatcacg atcaacgaca tccctaaaga gcaagacact tggcatttgc gaatcgaagt
    41881 gaaagacgat gccaaacatg tggggggctg tactttattt ggtaaaggat tcggtaatta
    41941 cgatcaggat atcaaattaa aagtttacta cagctgatat ttttcttttc tattttataa
    42001 aacaaaaaca gccaatccct atgaagggac ggctgttttt gctttattgt tatttccaga
    42061 aatcatccaa aacggaaatt ggcaaatgtc gtttatgttg tgtcttgttc caccatgctt
    42121 cgattttttc ttgtgcttga gcgggaactt cttttccttc tagataatca tcaatttcat
    42181 tatatgtgac acctagtgcg acttcatctg caatcattgg tttcccatct tcaagatctg
    42241 cagtcggaat ttttgtgtat aatttttccg gtgcattcaa tgcttgaagc aattgtttcc
    42301 cttgtcgttt gtccaaacgg aataatggca aaatgtcggc tccaccatca ccaaatttcg
    42361 tgaagaatcc agtaatgttt tctgcagcat gatctgtccc taatacggct cctgctcttt
    42421 ctccagctac ggcatattgc gtaatcatac gttgacgtgc tttgatattt cctttgttaa
    42481 aatcgctgat tcccacacct gcttcgctta atacttgtac ttgtgcatca acagctggtt
    42541 tgatatttac tcgcagacta acatcgggct ggataaagtc aagagcagct tttgcatctt
    42601 cttcatctgc ttgttcacca taaggaagac gaacagcgat aaattgatag ttttcatctt
    42661 ttgtttcttc acgcatttct tccatcgtta gttgcgccaa acggcctgct aaagtagaat
    42721 cttggccgcc gctgatacct agtacaaaag ttttaagaaa cggatatttt ttcaaatatg
    42781 ctttcataaa gtcaatactt ttacgtattt cttcttttgg atcaattgtt ggtttgacac
    42841 ctaattcatt gataatttct gtctgcaggc tcatttattc tcctcctatg cttctgtatg
    42901 aagttctgct acgctttttt tcattcgttc catgatagcc attttatggt tccagcattc
    42961 agtagacaga tcgactgggt atttttgcgg attcaagtct cgtttatact cttcccatag
    43021 tgaatctaga ttttcttttg tatattcttt gatttcgttt agtgcaggta attcgtaaac
    43081 tctttttcct tcgataaaga tatcttgtaa aactgggcga gctgtaaagt ctcgaacagt
    43141 tttattgata aacgtatgca ctggatggaa catgaagata gcttcttctt ctcgagggtc
    43201 ttcatcccat aatgtcacgt agtctccttc agatttccca tcaaaatttc gagtgatgcg
    43261 ccacacttgt tttttccctg gagtagttac tttttctgca ttgcttgata acttgatcgt
    43321 atccatcatt tttccttcat catcttcgat cgatactagc ttgaatactg caccaagggc
    43381 tggctgatca taggctgtga tcaattttgt cccaacaccc catacatcaa ttttagcttt
    43441 ttgcatcttt aaattcaaaa tcgttgcttc atccaagtca ttcgaagcat agatttttgc
    43501 ttccgtaaat ccggctgcat ctaattgttc ccgaacacgc ttagatatat aggccatatc
    43561 tccactgtcg atgcgtacgc cttggaagtt gattttgtca cctaattctt tcgccacgcg
    43621 aatcgcactt ggcacacctg atttcaacgt atcataagtg tcgactaaga acacgcagtc
    43681 tttatgggtt ttagcgtagg ccataaatgc ttcataatca tttccataag attggactaa
    43741 cgaatgagca tgggtcccgc tagctggtat cccaaatatc ttcccagcac gtacattgct
    43801 tgttgcatct gctccgccga tatatgccgc tctagttccc cagactgctg catccagttc
    43861 ttgtgcccgt ctagttccaa attccaatag tggatcttct ccgatgacag atttgatacg
    43921 agctgctttt gtcgcgatca acgtttggaa attgaccata ttcaacagtg ctgtttctac
    43981 taattggcag tgcgctaaag gcccttccac ttggatcaat ggttcatttg caaaaactag
    44041 ttctccttca cgagcagaac gaactgttgc tttaaattca aaattttgta aatactccaa
    44101 aaaaccttca ggatagactt ctaatccacg caagtaagca atatcggaat cattgaacgt
    44161 cagattctct aaataattga ctaaccgttc caatcctgca aaaaccgcgt atccgtggtt
    44221 aaaaggcatt tctcgaaagt aacattcaaa aacagcatgt aaatcagctc gacctaattc
    44281 ccagtatgtc tgcatcatat tgatttgata tagatccgta tgcagcgtca aactgtcgtc
    44341 tgggtaagta ttattcatct tgtattactc ctcttggcaa gttttcattt cctacccatt
    44401 ataacaaaaa aaattaatag gaaaagaaat cttccatttt ttctgaaaaa gcacatgttt
    44461 tacggatatt tcttgctaaa aatgaactcc atctgctaaa actatccttt cttattggta
    44521 tttaacctaa acgagaaaga atacttattt tattcaatgt atccgagaca cgaagaaatt
    44581 agcgttttct atttttctta cttgataaaa taagtagatt ttcctactac aatcgtagcc
    44641 gttaagatac cgatgattga acaaatataa ccaaatgtct ctacaacata atcaatggca
    44701 aaagtttttg aagcaataat acgtctttcc ttttttgctt cttatcccca aagaaacgga
    44761 tataacttat tatgtattga atttttttgg aaaggcgaat tatgacaaga tataccttgc
    44821 gttgaaaaaa ctcgcggaaa aaatatcaca cgtttttaaa aagatacaaa gtaagaaagc
    44881 agctgtcaat agaatggaaa caaaaatggc gagacatcaa tcagtctcgc cttgtttttt
    44941 attgtataca ttagatatgg agcaggttac cataaagatg attattagtc gttattaaat
    45001 agaatccttc gtattttttt gttctatcac tacaatatct ttatttggac gtttgaattg
    45061 tattccggca cgttctagct cctgtttcac tcctacatgg actttgacat cttctcgtgt
    45121 catttttaaa acgtgatgaa tcgcatagtc gatttcttgc cttgtgattt ttttcttcgt
    45181 attgatcatc acgacttgct ttttatcata tgtgtctacc aatagcagtt gtgttcgatc
    45241 taacgaaaat aatttgtaat aattaatagc cgtattccca aaaaagaatt ccactacttt
    45301 aggataaaat gtcttaatat ttaaattact attcacaggt gtttcgatta gcttcatctc
    45361 attcctcttt tctatatagt cgatttagat ccaatcttaa gttatgttac catctttcct
    45421 agcattaagg aatcggttac aattgaacct ttaaagacaa aaggatttac cttttatcac
    45481 ttaatagtat aacataattt catgagaaaa aaatacaatt ttttcttgac catctcgtta
    45541 cgtaaaggat tatgcttaca tttgtcagga ggaaaaataa tggagtacac aataaagaaa
    45601 atgtcagaga tatcaggagt cagtccgaga accttgcgct tttatgatga gattggactg
    45661 ctaaaaccag ctaggatcaa ttcttcaggt tatcggattt atggcaaaaa agaagtcgat
    45721 cgtctgcaac acatcttatt ttatcgaaca atggcattca agttggatga tatccaagag
    45781 gtcttagaca atccatcttt tgaccaccag aaagctttaa tcaagcatca gcaaatgttg
    45841 ctggaaaaac gggcacaaat cgataccctc ttgacaactg tccaacagac acttgatatg
    45901 tacgaaggag gaagaaaaat gagtgacaaa gaaaaatttg aagggtttaa acaacaaaag
    45961 ttgaaagaaa atgaagaatc ctatggacaa gagattcgac aaaaatacgg aaaaaaaaag
    46021 gttacagcat ccaatgaaaa atggatgaat atggataaag aaacgtatga agaaatgcaa
    46081 actgttgaaa agcagatgtt atttgatctc actctttact taaaagaacc agaagatgaa
    46141 agcttagcag accgtatctt ccaagctcat aagaagtggt tatcttacag ctggcctgaa
    46201 tatcagccaa aagcgcataa aggactagga atgatgtacg aagccgatga acggttcact
    46261 gcatattatg acgagcgaag tggtcatggt gcagcaaaag ctttaaatga aattatccaa
    46321 aaaaaggcgt aatcttttac atgatccaac aaaaaagtac acgtaaaatg acctaataac
    46381 aaaacagcag acacccaagt tagattttta tgctaacttt tggtgtctgc tccattcttt
    46441 cacaagtttt ttcatcctac tttaaatata ttcatcgttg aataaacgta taaggtattt
    46501 tcactttatc ctttttttct gtaagaaaca attgaaatgc catttctccg caagcttcca
    46561 gatggtggtc gatggttggg aaaccaagaa gctcgctgat cagcaaatta tcgcgtccaa
    46621 tcaccactgg tgtcttatct tgcagatagc tttgcaagat gcctgctgca accatatccg
    46681 aatttgtaaa gacaccatca actccaagct ctttaaaaaa gaagcctgct tgttttcctt
    46741 tctctgcatc tatacaatcc caaaaaacaa gtgattcatc aaaagatgga aaaagttgat
    46801 gacatagctg caaagtgatt tttgagttat aacttagcct gccacttcgc cccaacgtga
    46861 tgcccaattg ctttatacct tgctgcttca aataagtaag accttctgta attgattgtt
    46921 ctcgatcaat aaagacacag cttgcctgtt ttcccttgat ttcttcacaa aaaactagag
    46981 gtccatattt ttgatatgcc aataaatcat ctagtgtatt ggcacgagac gtaatgatca
    47041 atccatcgaa agctttagct gcaaattctt caagatacct tcgctctttt tctttctgat
    47101 agcccgtcgg taaaagtgtc actttgtaat cataatcaaa cgctgcttca atgatcccac
    47161 tgatcaactg atcaaaatac ggatgattgg tataaggcag aatcactcca atgcttttcg
    47221 tttttccata acttaactgt ctggcatttg cattagggat ataatcgact tctttcatga
    47281 ccgcaagtat ttctttcctt ttttcctcat ctacatatgg atgtccattg attacacgtg
    47341 aaatagtaga aactgaataa cctgtgagct ttgcgatatc tcgaatgttt gccactgctg
    47401 cttcgccacc tatacaaaaa gattttcttg taaatagtct accacagctg gaccgcattc
    47461 aaaaaactga tacatttttc cttcatgcga aaaactgatc cgctgcccgc cccaatatat
    47521 atcttttgtc aaattttcga tttcattcat tggtatttct tttactatat tcctattcaa
    47581 cccttgaaaa ctgtacaacg ttaatacgga tcccttgaac tcgacaaacg cataaaaata
    47641 attgttcgtt gtgccatctg ttatctctac ataagtattc attgcaagcc ctccttttga
    47701 tactttgagt atagtgtatg aaacgcgtct catagcaagg agtttgtttt gccaaagaaa
    47761 agttttattc ctctttcatt attaccaaag tctcttattt taaattgaat aca
//
LOCUS       DAHHZJ010000005        47460 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.005, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000005 DAHHZJ010000000
VERSION     DAHHZJ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.005"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(45..779)
                     /locus_tag="NBL02_000188"
     CDS             complement(45..779)
                     /locus_tag="NBL02_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HCD2570904.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1106..1516
                     /locus_tag="NBL02_000189"
     CDS             1106..1516
                     /locus_tag="NBL02_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCD2570905.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1509..2210
                     /locus_tag="NBL02_000190"
     CDS             1509..2210
                     /locus_tag="NBL02_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HCD2570906.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2210..3796
                     /locus_tag="NBL02_000191"
     CDS             2210..3796
                     /locus_tag="NBL02_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD2570907.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3815..4036
                     /locus_tag="NBL02_000192"
     CDS             3815..4036
                     /locus_tag="NBL02_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HCD2570908.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4033..4794
                     /locus_tag="NBL02_000193"
     CDS             4033..4794
                     /locus_tag="NBL02_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HCD2570909.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5174..5683
                     /locus_tag="NBL02_000194"
     CDS             5174..5683
                     /locus_tag="NBL02_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HCD2570910.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5758..6297)
                     /locus_tag="NBL02_000195"
     CDS             complement(5758..6297)
                     /locus_tag="NBL02_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HCD2570911.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6437..7048)
                     /gene="rpsD"
                     /locus_tag="NBL02_000196"
     CDS             complement(6437..7048)
                     /gene="rpsD"
                     /locus_tag="NBL02_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCD2570912.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7310..8161
                     /locus_tag="NBL02_000197"
     CDS             7310..8161
                     /locus_tag="NBL02_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCD2570913.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8189..8824
                     /locus_tag="NBL02_000198"
     CDS             8189..8824
                     /locus_tag="NBL02_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HCD2570914.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8844..9617
                     /locus_tag="NBL02_000199"
     CDS             8844..9617
                     /locus_tag="NBL02_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCD2570915.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10025..10822
                     /locus_tag="NBL02_000200"
     CDS             10025..10822
                     /locus_tag="NBL02_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HCD2570916.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10800..11213
                     /locus_tag="NBL02_000201"
     CDS             10800..11213
                     /locus_tag="NBL02_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCD2570917.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11197..13842
                     /gene="mutS"
                     /locus_tag="NBL02_000202"
     CDS             11197..13842
                     /gene="mutS"
                     /locus_tag="NBL02_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCD2570918.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13860..15968
                     /gene="mutL"
                     /locus_tag="NBL02_000203"
     CDS             13860..15968
                     /gene="mutL"
                     /locus_tag="NBL02_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCD2570919.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15990..16550
                     /gene="maf"
                     /locus_tag="NBL02_000204"
     CDS             15990..16550
                     /gene="maf"
                     /locus_tag="NBL02_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HCD2570920.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16635..18623)
                     /gene="treP"
                     /locus_tag="NBL02_000205"
     CDS             complement(16635..18623)
                     /gene="treP"
                     /locus_tag="NBL02_000205"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HCD2570921.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18846..19559
                     /gene="treR"
                     /locus_tag="NBL02_000206"
     CDS             18846..19559
                     /gene="treR"
                     /locus_tag="NBL02_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HCD2570922.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19636..20082
                     /gene="msrB"
                     /locus_tag="NBL02_000207"
     CDS             19636..20082
                     /gene="msrB"
                     /locus_tag="NBL02_000207"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCD2570923.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20252..20854
                     /gene="ruvA"
                     /locus_tag="NBL02_000208"
     CDS             20252..20854
                     /gene="ruvA"
                     /locus_tag="NBL02_000208"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCD2570924.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20867..21868
                     /gene="ruvB"
                     /locus_tag="NBL02_000209"
     CDS             20867..21868
                     /gene="ruvB"
                     /locus_tag="NBL02_000209"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCD2570925.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21897..22475
                     /locus_tag="NBL02_000210"
     CDS             21897..22475
                     /locus_tag="NBL02_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCD2570926.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22527..23009
                     /locus_tag="NBL02_000211"
     CDS             22527..23009
                     /locus_tag="NBL02_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HCD2570927.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23126..23500
                     /locus_tag="NBL02_000212"
     CDS             23126..23500
                     /locus_tag="NBL02_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2570928.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(24178..25530)
                     /gene="brnQ"
                     /locus_tag="NBL02_000213"
     CDS             complement(24178..25530)
                     /gene="brnQ"
                     /locus_tag="NBL02_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCD2570929.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
     gene            25677..26267
                     /locus_tag="NBL02_000214"
     CDS             25677..26267
                     /locus_tag="NBL02_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HCD2570930.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            complement(26395..27744)
                     /locus_tag="NBL02_000215"
     CDS             complement(26395..27744)
                     /locus_tag="NBL02_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD2570931.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(27912..28652)
                     /locus_tag="NBL02_000216"
     CDS             complement(27912..28652)
                     /locus_tag="NBL02_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCD2570932.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            complement(28665..30257)
                     /locus_tag="NBL02_000217"
     CDS             complement(28665..30257)
                     /locus_tag="NBL02_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCD2570933.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            complement(30825..31121)
                     /locus_tag="NBL02_000218"
     CDS             complement(30825..31121)
                     /locus_tag="NBL02_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2570934.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            31297..32022
                     /locus_tag="NBL02_000219"
     CDS             31297..32022
                     /locus_tag="NBL02_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCD2570935.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            32015..32857
                     /locus_tag="NBL02_000220"
     CDS             32015..32857
                     /locus_tag="NBL02_000220"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HCD2570936.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            32860..33381
                     /locus_tag="NBL02_000221"
     CDS             32860..33381
                     /locus_tag="NBL02_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HCD2570937.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(33634..34197)
                     /locus_tag="NBL02_000222"
     CDS             complement(33634..34197)
                     /locus_tag="NBL02_000222"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCD2570938.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            34448..34717
                     /gene="rpsO"
                     /locus_tag="NBL02_000223"
     CDS             34448..34717
                     /gene="rpsO"
                     /locus_tag="NBL02_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCD2570939.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            34905..37031
                     /gene="pnp"
                     /locus_tag="NBL02_000224"
     CDS             34905..37031
                     /gene="pnp"
                     /locus_tag="NBL02_000224"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCD2570940.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            37541..37807
                     /locus_tag="NBL02_000225"
     CDS             37541..37807
                     /locus_tag="NBL02_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCD2570941.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            37984..39942
                     /locus_tag="NBL02_000226"
     CDS             37984..39942
                     /locus_tag="NBL02_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HCD2570942.1"
                     /translation="MTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            40296..41597
                     /locus_tag="NBL02_000227"
     CDS             40296..41597
                     /locus_tag="NBL02_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCD2570943.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(41727..43097)
                     /locus_tag="NBL02_000228"
     CDS             complement(41727..43097)
                     /locus_tag="NBL02_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HCD2570944.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVNPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(43279..43602)
                     /locus_tag="NBL02_000229"
     CDS             complement(43279..43602)
                     /locus_tag="NBL02_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HCD2570945.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            complement(43927..44535)
                     /locus_tag="NBL02_000230"
     CDS             complement(43927..44535)
                     /locus_tag="NBL02_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HCD2570946.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            44865..45581
                     /locus_tag="NBL02_000231"
     CDS             44865..45581
                     /locus_tag="NBL02_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HCD2570947.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            45594..46289
                     /locus_tag="NBL02_000232"
     CDS             45594..46289
                     /locus_tag="NBL02_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HCD2570948.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            46373..47002
                     /gene="udk"
                     /locus_tag="NBL02_000233"
     CDS             46373..47002
                     /gene="udk"
                     /locus_tag="NBL02_000233"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCD2570949.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
BASE COUNT        15383 a         8787 c         9878 g        13412 t
ORIGIN      
        1 ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc cataaataat
       61 aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac tggtacccat
      121 gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg tacaagacgt
      181 ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag tgaagcaact
      241 aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc ttggaagaat
      301 ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa gaattcattg
      361 acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc taacacatgg
      421 tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat caattgtcca
      481 atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca agggaataga
      541 ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct gattccacca
      601 aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag catccagtca
      661 ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt acctagaacg
      721 attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga gtaatccata
      781 aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg aaaagcatcg
      841 gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat aaaaaaatga
      901 attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt taaaagttct
      961 ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt tttctcaaaa
     1021 attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta gtaaaatgaa
     1081 agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca ttattatttt
     1141 cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc ctggaagtat
     1201 cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc ctgaaaaaat
     1261 cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc ctgctggtgt
     1321 aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac tgggagcagt
     1381 cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag gtgtagaagc
     1441 tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg aaatggagga
     1501 gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg actttaggag
     1561 tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg aatcctctcg
     1621 tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc tataaagact
     1681 ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg gcacttgcca
     1741 tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta atggctgcga
     1801 ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac ttcttctctc
     1861 ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct atttctgtcg
     1921 atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt gtctctacag
     1981 gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc gaaagtccag
     2041 ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca aaagcaatcg
     2101 agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac ggaatattga
     2161 ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga tgcgaaaacc
     2221 actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga aagacgtgta
     2281 tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga ttctcctcct
     2341 agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag ttgttcagct
     2401 ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc agcagtttag
     2461 cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact tttatgatac
     2521 gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta cgctgaaatt
     2581 ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat ttgcgcaaaa
     2641 gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac agcaactgca
     2701 aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag aagaaatcat
     2761 tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct ggccatttga
     2821 aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt tttttaatgt
     2881 tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct tttttttaag
     2941 aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga tttctgacaa
     3001 tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt attttcaagt
     3061 agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat attatagtga
     3121 tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac cgccctttac
     3181 agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg ggctgccagc
     3241 agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt tcgcctatct
     3301 ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta atcatttagt
     3361 tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta gacacaaaca
     3421 tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc tgactgtgtt
     3481 taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt taatgacaga
     3541 tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt tccgtaatga
     3601 gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc ttctgatttc
     3661 gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg tgactgaaga
     3721 actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc aaaaacaaaa
     3781 actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa ttttaccaga
     3841 attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac tttatgttgt
     3901 tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat tgatcaaaac
     3961 aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag cagatgattg
     4021 ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt atttatcaca
     4081 ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca agatgctttt
     4141 gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg atttcctgaa
     4201 acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca agcggtatcg
     4261 aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg gatattggat
     4321 gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta tgagacgaat
     4381 gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg ccaaaaaagc
     4441 ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg tgggatcgct
     4501 tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct ggattacgca
     4561 ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc aatgaatgcg
     4621 gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac accagtcaag
     4681 cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc tcctcaagca
     4741 agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa ataaccttgt
     4801 gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg atgataaaaa
     4861 aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa ttctttttaa
     4921 taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa acgatattca
     4981 aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag ctatttttat
     5041 ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc gacacttcag
     5101 ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt atcttgcaaa
     5161 ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga caacgacaga
     5221 tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg ttttgtctgt
     5281 ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc tgtccttgta
     5341 taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata ttgcttctcc
     5401 tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat tgcttatcaa
     5461 ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga cagcatgcgt
     5521 atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc aactaccttt
     5581 tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg tcggtgggat
     5641 aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg tttaatttaa
     5701 aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct tttttgatta
     5761 atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat aagctgctcc
     5821 agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa atgcagttgt
     5881 aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg cagcaagaga
     5941 gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt cgatcatcca
     6001 accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg gcccaaataa
     6061 aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc caagtaaaag
     6121 ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg ttaatccaat
     6181 agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct gagaaaggac
     6241 agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc ggttcataac
     6301 gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa acaaagtgat
     6361 tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc ggtctttttc
     6421 aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga gcttcgtcga
     6481 tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc aatttttcag
     6541 cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct tttactgtta
     6601 cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg tggtatgaag
     6661 ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat tgacgtgctt
     6721 gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt tgttctagta
     6781 agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt acgaacaagt
     6841 tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt tcagttaatt
     6901 gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct ggtgcgtaag
     6961 ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga cgagaaattt
     7021 tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat aagtttttgg
     7081 agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat cttcaccttt
     7141 gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat gagttattat
     7201 ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca cgaatgtcaa
     7261 gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa tgagagggta
     7321 tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc cggtatcacc
     7381 attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg aaaacagctt
     7441 ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag gaacggctgt
     7501 ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa agattttata
     7561 cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg aattaggaac
     7621 atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt taagtaaggc
     7681 agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat tatgtggatt
     7741 tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta tgttagaatc
     7801 aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga tgtttgcgaa
     7861 tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg tcggaaaagt
     7921 gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac acgtgatagc
     7981 gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg atggaatgac
     8041 agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata ttggtggcgg
     8101 tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct atgtggacta
     8161 aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa aaaatgagga
     8221 aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg gagtacctaa
     8281 gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt tatctatttc
     8341 tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt atcagtatgt
     8401 agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact tagtcgtatt
     8461 gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga gaaaagtata
     8521 cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat tgtttgaaaa
     8581 aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt ataaacagca
     8641 aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa aggaattctt
     8701 tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg ccgattgggt
     8761 aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa gtaagtattt
     8821 ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat attgatggaa
     8881 cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg gaaaaattac
     8941 gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat gcgcaagaga
     9001 tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc ggttttttag
     9061 accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg ttcgttcaag
     9121 aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc aagcgttctt
     9181 cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct gtcttgcctg
     9241 aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca tttttcgatc
     9301 aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc tggcatgaga
     9361 acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg cacttagcag
     9421 aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa aacgaccgtg
     9481 aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca gaagtacaag
     9541 cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag gcattgaaag
     9601 agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt aataagccca
     9661 gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc gcagatgcaa
     9721 gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc aagaaataag
     9781 aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat ttctgaagaa
     9841 gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg tcatagtccc
     9901 atttttttat tttatttttc aaaactaaga aataccagga aagagttcct tttgtttgac
     9961 cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag aagaggaatt
    10021 tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag agatgattac
    10081 tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg taaatggtga
    10141 gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt tgcaagacgg
    10201 cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct ttgagtttgt
    10261 aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc ctggacaagg
    10321 gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc aggctcgttc
    10381 ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag aagaagccag
    10441 aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg aaaaacaagc
    10501 aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata cacacgttca
    10561 gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg ttgggatgac
    10621 aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa agtttctgac
    10681 tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa gcggctgtat
    10741 cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc agatcaacga
    10801 tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag ccggaaattc
    10861 aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc attcgctaca
    10921 aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga gaaaaaatca
    10981 aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca gcagaaaaag
    11041 aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg cttgttgtag
    11101 cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca actatgatac
    11161 aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac taaaaatact
    11221 cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt tttgttctat
    11281 cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc acagcttttg
    11341 gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat gtgcggtgta
    11401 cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta taaagtagcg
    11461 atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg cgaagtgatc
    11521 cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa agacaacaat
    11581 tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc cgatttaagt
    11641 acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa tgaagcctct
    11701 gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct tcgtgaacag
    11761 ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga aaacgcggaa
    11821 tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac cggcaaacta
    11881 ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa agcagtagaa
    11941 taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct agaattaagc
    12001 caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga tgaaacaaaa
    12061 acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat ccaggcaaaa
    12121 caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt tgaacgtatc
    12181 gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg tcgagtagct
    12241 ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga acaagtgcca
    12301 atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct gctttcagaa
    12361 atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga tgatccgccg
    12421 ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct cgatatgtac
    12481 cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa agagcgtcaa
    12541 gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta ttatatagaa
    12601 attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa acaaacttta
    12661 gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca aatcctcgaa
    12721 gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag agaagaagtc
    12781 aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt agatgtgcta
    12841 caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact tgtcagtgac
    12901 aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt attaggacat
    12961 caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt gctgattaca
    13021 ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac tgttctgatg
    13081 gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt tgatcggatt
    13141 tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt tatggtagag
    13201 atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat tttgtttgac
    13261 gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc aatcatcgaa
    13321 tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca tgaactgacg
    13381 gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt tgaaaaagac
    13441 ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag ctacggcata
    13501 catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc agatatcctg
    13561 catacgctag aaaatggcga taacggacac catactgttc cagctgaagt agaagagaaa
    13621 actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa tcaagaccat
    13681 atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga attatccgaa
    13741 aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat gacgccaatg
    13801 gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg cggatatcga
    13861 tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg ggagaagtag
    13921 tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac gccggcagta
    13981 ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg attgataacg
    14041 gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg acaagtaaga
    14101 tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg gaagctttgc
    14161 caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa gaaaaacaag
    14221 gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca ccactgcgtc
    14281 aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc ttgaaatacg
    14341 tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga ctggcattga
    14401 gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg gcaactgctg
    14461 gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc gcaaaaaaaa
    14521 tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt tcattgcctg
    14581 aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt tttatcaaga
    14641 acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg gtcggtcgtt
    14701 tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta aatgttcatc
    14761 cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta attagtaacg
    14821 ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat aatctacggt
    14881 ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat ttgtcctacg
    14941 cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga gagacaggta
    15001 cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat aagtctgtgg
    15061 aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca gaaaaaacag
    15121 cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa acagtggaaa
    15181 aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat gaagaacaag
    15241 cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca ctccatcctg
    15301 aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag aaattagaaa
    15361 cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg cacggaacgt
    15421 atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct gcccaagagc
    15481 gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat ctgcaagaat
    15541 tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta aaagagcaaa
    15601 aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac agctttattg
    15661 tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt cgggagatga
    15721 tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa gcaacagcaa
    15781 tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa gcacaagcac
    15841 gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc catggccgtc
    15901 cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga atccaagacc
    15961 cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc caatcaccaa
    16021 gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct gctgatatcg
    16081 atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg gctgaagaaa
    16141 aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt gccagtgaca
    16201 cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct gaggctgaat
    16261 tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt gttttaaaaa
    16321 caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat cctttgacag
    16381 acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca ggggcatacg
    16441 gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat tacagtatcg
    16501 taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa acaaaaaagc
    16561 ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt gaaaagatta
    16621 cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc cgggcgaaca
    16681 agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa agcaacaata
    16741 atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc aatcagggtc
    16801 tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc ctttagttca
    16861 acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag aataccaatg
    16921 gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata gactttgccg
    16981 tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac tgggtcattt
    17041 acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc ttttgttgca
    17101 actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt tgcgccattt
    17161 gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat cttattcaaa
    17221 attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc aatgatcatt
    17281 gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg caaaccgcct
    17341 actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc taatccagag
    17401 ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa catagcaggt
    17461 tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc ttctttttta
    17521 ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat atttgataaa
    17581 gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat atcgatggca
    17641 ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc gaatacggca
    17701 ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga gatccagctt
    17761 ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt tggtaacaat
    17821 gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg gaagaagatt
    17881 tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc ttgataacca
    17941 atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc ggctgtggaa
    18001 gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa aacgattcct
    18061 aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac tggaagaaaa
    18121 tggaaaattg cttcaccggg taaccacaaa aaggcattga caccattcca aaacttcgag
    18181 tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac atttctgaat
    18241 ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat ttctgctaaa
    18301 acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt tccttgttct
    18361 ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt agaaacatcg
    18421 tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac gctaggtata
    18481 tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg catacgagtc
    18541 gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa cagcttctcg
    18601 gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg tctatgcaag
    18661 ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt attaaaaagt
    18721 acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa aaataggaac
    18781 attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg agtgagggaa
    18841 aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt ttagcgaaag
    18901 aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt tatggtgttt
    18961 cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat attcaaaaaa
    19021 aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct atttctggat
    19081 tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg atcatccata
    19141 cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg gagatcggtg
    19201 cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt atattagaca
    19261 ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca caaggatcga
    19321 tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa aaggagatca
    19381 cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat gacacttatg
    19441 cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag tattctgagt
    19501 cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga aaagtgtaaa
    19561 tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga taggatcaca
    19621 aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact gaaagaaaag
    19681 ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc atttagcggg
    19741 aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg tgaaccgtta
    19801 ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag caaaccaata
    19861 acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg aatagaagtt
    19921 cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc agtagatcaa
    19981 ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata tgaaaagatg
    20041 gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata cacaaagagc
    20101 ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg attgctattt
    20161 gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca ataaagggaa
    20221 cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag gtgtggttac
    20281 ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc agatcgcact
    20341 aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt atgtccatca
    20401 ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag aaaagcaact
    20461 gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg caatcatggc
    20521 aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca cttatttgac
    20581 gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc taaaaggaaa
    20641 attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa acacaaaaga
    20701 cgctacagct ctatctgaag caatggaagc tctctcggct cttggttaca gtgatcgaga
    20761 aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg atgagtatct
    20821 acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga cagatgaata
    20881 cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc ttcgaccaca
    20941 gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga tttacatcga
    21001 agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc ctcctggatt
    21061 aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata ttcgtacgac
    21121 gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg aactggaagc
    21181 aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag aagagatgct
    21241 ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc caaccgcaca
    21301 tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac gggcaggtat
    21361 gctttctgca cctttacgag atcgatttgg catcatttct catatggaat attatgacga
    21421 acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag agatcattga
    21481 agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg ctaatcgctt
    21541 gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg atcgtcctat
    21601 agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt atgttgacca
    21661 gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt taagtacgct
    21721 ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac cgtatctgat
    21781 ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt atgcctatga
    21841 acattttggt tacgactatc atatatgatt tttgctttta gattggagga aaatgaatga
    21901 gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg tgccaaacgc
    21961 aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc aatcgacaaa
    22021 cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat tgtgaggatg
    22081 cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag caagcattga
    22141 aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg ctttctgacc
    22201 cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc aagatttttg
    22261 aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat gctcgttttt
    22321 tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat gaagaaacac
    22381 cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta tattcttatc
    22441 gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg ttggaaaagc
    22501 aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt ttgggaaata
    22561 tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta gaaggaaggg
    22621 aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga aagacaccgc
    22681 accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat atggttgctc
    22741 ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg gatcaagaaa
    22801 atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc catttgtttt
    22861 taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat accaataact
    22921 ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat atttggattg
    22981 atgaaaataa aataacaaac aatttgtaaa gttaacgctt tctttttaag gcgatgttag
    23041 agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa gtaacacttt
    23101 cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa tgaagaagct
    23161 tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa atgacactga
    23221 agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc aaaaagctgc
    23281 tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta gcaagtatga
    23341 aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga tgatccgcct
    23401 acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa aaataacaat
    23461 cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag atagaaaagc
    23521 acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc tttaagctga
    23581 tatcagtaaa acggaacgtt ttaggatgag tatcacagca tgacagcatt gggttgatta
    23641 gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct agccggggag
    23701 ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt tggtatgctt
    23761 atataatagt cactagttgt gaatattttg tgacgaaagt tttcgaaagt tgttaagttt
    23821 ttttgcgaaa actattttta tatcatatat atgtctaaaa tctatattat aaaagaaatg
    23881 ttgtttgatc agcttctctt cggtattagt tcaaaacaaa acggcaaagt atatggagct
    23941 actttgccgt tttgtttctc taatactcag gtcagtttat agacgctcaa taaacggtgt
    24001 tcaaaagcca gtttctcggt attaagtcaa atttatgcaa aaatgtgaga agccattttc
    24061 aaaaaatatt tcttaatact cgaaactgag cggctttttc acgacctctc aataaacagt
    24121 gttcaagaga cagcctatcg gcaataaggc tgaacgtctc tttcacaacc tcttttatca
    24181 atctgctact tcttctttct tagcgaaact ccaaatcagt ccgataacaa aaccgataac
    24241 tgctggtacg atccacccca tccccaattt gaaaaacggc aagtagcttt ccgcaacata
    24301 taaaagattt tgaacaatag gtgtttgttt gatagtttct gggcaagcgt tcaacccatc
    24361 aaaaatcgaa gcaatcaatg taaaataagt agtcattcga taaacagccg tacgctgctt
    24421 aaacaaagga ccaactaaca ctagtaaaat cagtgtcatt gctaatggat agatgaacat
    24481 caaaacaggc aacgaaaatt gaatgatatt cgtcaagcct acgtttgccg ctaaacaagg
    24541 aaggatacta gcaagtgcaa caaagaatgc gtaatttctt tgcggaaaca aatctgtgaa
    24601 tgtttctgca aaagctgtaa tcaaaccaat accggtcttc aagcaagcta atataacgat
    24661 aaatgctaat aagatactgc cataagtacc taaataatga tcagcaatct gagccagcgt
    24721 gatcccgcca ttttcactta gagcaaagct acctaagctc atagcaccta aataagcgag
    24781 caaagtataa atgattccca tcaatataat gctgatcgca ccagatttga tcgtgtcttt
    24841 agcgatttca gcaggttttg ttacacccat gttttggatt gttgtgacaa tgatgatccc
    24901 aaatgctaaa gcagcaagcg catctaatgt gttatatcct tgagtaaacc ctgtgaaaaa
    24961 agcatgctct tgataatttg gctgaatagg cgcttgattt acagaaccta gtggattcaa
    25021 aaaagcaagg agaagcaaag cacctaataa aaccaaaaat gcaggattca ggaactttcc
    25081 aacataatct aatatctttg tcggttttct tgaaaaccac caagccagca caaaaaatac
    25141 aatactgaaa attgctagat atagcgtctg tctgctagga tcgataaatg gtgctaaacc
    25201 aatctcgaaa gatgtggttg caaggcgagg taaagcaaaa aacggtccga taactaagta
    25261 aagcaagatg gtaaaaacca tcgcatatgt ttgatttatc cgttttgcta attcatatac
    25321 acctgagctt ttcgatacac caatggcaat cacccctaag aagggaagac cgataccggt
    25381 aaccaagaaa cctagattcg cccaaaaggt agcagcgcct gcttcttgac ccaaatgaac
    25441 gggaaagata agatttccag caccaaaaaa tagaccgaaa agcatggagc ctataaaaag
    25501 atagtcttta aatgataatt tttttgtcat acataactcc tttacggttt ttttaaaagt
    25561 aacaaaattt cgaaaaatat gcaacaattt tcagaaaatt tcctatataa aacgacggtt
    25621 atttaataac gtaagtgata aaataaaagc gctgaatcat ctgaggagga atcatcgtga
    25681 caaagtcgag attagaagca tttactgatg gagtggtagc cattgttttg agagttcttg
    25741 tattagagat caagatccct aatacatcta gctttcattc tttatgggtc atccgaaata
    25801 cattattagc ttataccatc agctttatat ttgttgcggt gatctgggtg aaccatcatc
    25861 gtatatttca gatggcagaa cgaattaatt accgagtggt ctggtcgaat atctttttgc
    25921 tcttttggct gactttatgt ccttctgtta ctagttgggt cgggcgtaac cctgaagctt
    25981 tttggcctga gatggcttat gtcttagtat acacgatgtg gagtttttct tttggtatct
    26041 tgatgaggca aatcatgaaa gcaaataaac cagattccca tgtcgtgaga gttttgtcaa
    26101 aggaccgtcg aagtcttatc tccatgatga ttaacttatg cttgattgcc ggtgtctttt
    26161 ttgtaccgtt gatcgggtta ttcggacgtt tttttgtatc tggtatttgg attttttcct
    26221 ataaaaaagc agatgcctat tatcagcggt tgtttccggg gaaatagaaa agaggctggg
    26281 acagaagctt ttaactccaa gaaataagcc gaaatttcac aaaaatttaa gaagcaattt
    26341 tcgtaaattc cttcttattt ctcgaagctg accacttctg tcacaactac tttcttactt
    26401 cttacgttca tatttcatat gatagattag taatagaagc aagaaccaca caggtgttag
    26461 taataaagcg attctcgtat cttttcctag gaacaagacg acacacacaa aggccatgta
    26521 aagaaatacc agataattac taaatgggta caacggcatt ttaaatgaat gagctttttt
    26581 gccgaggata cttttacgat atttcatgtg cgtgtagaca agcatactcc agataaacaa
    26641 gaaacaagta gtggcaatac tgcttactag tgtgaatact tcactaggca tcacgtaatt
    26701 caagataaca gaaatgaaaa tgacgatagt agaaaatgtc aaagcattcc ctggcacatg
    26761 atgtgttgtt aactttttga attttttagg ggcactgcct tcttgcgcta aagaacgaag
    26821 catacgtcct gtactatata ttgcactgtt gcaggcagaa gctgctgcac ttaatacgac
    26881 taaattgatc aaggaagcag caatcgtgat cccaatttcc gaaaatactt ctacaaatgg
    26941 actgttatct ggattcaagc tgttccatgg atagattgcc ataatgacaa aaagagatcc
    27001 taagtaaaat aagatgatcc tgataggaat attattgata gcttccggca acactttttc
    27061 aggattttct gtttctccag cgactaatcc aactaattct atcccagcaa aagcaaaggt
    27121 taccatttgg aaagctaaga taaatccttt tgcaccagtt ggaaagaatc cgccatgaga
    27181 ccatagattc gtgatactgg ctgtgccaat atctgtcgta tagtgggtga ggatcatata
    27241 agctcctacg aggataaagg caatgatcgc tacaactttg atcaatgcaa accaaaactc
    27301 taactcacca aacaatgaga cagccaccat attcaagcaa agaagaagac ctaatgcaat
    27361 cagttcagga agccactgag gaacaccagg cgcccagtag cgtacataca ttccgatggc
    27421 tgttaaatcg gccattgcaa tcgataccca gcagaaccaa tacgtccagc cagtgatgaa
    27481 agccgctttt ttgccaagat attctgcaac aaaatccaga aaagaatggt tattggtgtt
    27541 tgataacaga agttcgccta acgcacgcat aatgaaaaaa cagatgcatc cagtaataag
    27601 ataggcgagc aatatcgaag ggccagctag ctggatagac tttccggaac ctaaaaacaa
    27661 ccccgttcca atcgctccgc caatagaaat cagttggaca tgtctatttt tcagtccacg
    27721 ctgcagttct tgctcttcat tcatttttaa atcatcctaa ctaaaaaagt ttaattactt
    27781 ccatattaaa agagttttct gaaaaacaca actaagattc caaaattttc agaaatttac
    27841 cttttatgag taattcataa aaaagaactg cggtaaacac cgcagctctt tgtcccaatc
    27901 agctattgat cctaagcatt taaaatacgt gaaaggaatt cttttgtacg ttcttctttc
    27961 ggatggacaa agatatcttc tggtgtacct tcttctgcaa tcacaccttt atccataaaa
    28021 atgacacggt cagatacatc ttttgcaaat tccatctcat gcgtcacgat aagcattgtc
    28081 aagccagtat gtgctaagtc tttgatcgta ttaagcactt cgccaaccat ttcagggtct
    28141 aacgccgaag taggttcgtc gaatagcaat acatctggat tcattgataa tgcccgagca
    28201 attgcgacac gctgtttctg tccaccagat aattgtgctg gtttggcttc gatataacga
    28261 tccataccaa ctttttccag attttcgatt gctacttttt tggcagtttc tttatctcgt
    28321 ttcaggacag ttgtttgacc agacatacag ttttccaaga cattcatatt gttgaacagg
    28381 ttgaaggatt ggaaaaccat acctagattc gttcgatatt taggtaaatt atatttagga
    28441 gctaatacat tttctccatt ataaatgatc tcgccgcctg ttggtttttc cagtaaattg
    28501 atacagcgca agaaagtaga tttaccagaa ccggaagaac cgatgatcgt cacaacttca
    28561 cctttattga ctgttacgct gatatctttc aatacttcat tttcaccgaa gctttttctt
    28621 agatgtttga tttcaataat tttttcattc atgattcttc ctccttattc ctgcaaattt
    28681 gaattgtcga tttcttcaag cttggtataa gctgaaggac catcgatttt cttttctacc
    28741 gcccgcagga ttctggtgat cgtaaaggtc atgactaagt agatgatccc gacgatcgta
    28801 aatgtttgga agaattggaa attcgcaccg gaagcagagt ttccttggaa gaacaaatca
    28861 gctacgccga ttacacttaa aactgctgta tctttgatat tgataacaaa ctcatttcct
    28921 gttgctggca agatattccg taaaacttgt ggaataacaa cttttctcat cgtttggcca
    28981 tgggtcatac caatggcttg cgcagcttcg aactgccctt catctactgc aaaaatcccc
    29041 ccacgaacga tctctgtcat gtaagctcca gtattgagag agacaataaa caaagcggca
    29101 atcgttcggt tcaatgaaat gccaaaagcg agtgccagac cgtagaagat gaccattgct
    29161 tgtaccatca tcggtgttcc gcggaatact tcgacataga tagaaagaag tgcatttccg
    29221 attttttgga agaagcgagt cagtgcattt cctgattcag gtaccgaacg gaagacacca
    29281 atcagtaatc caagtgtagt accgacaact gtcccgatca atgcgatgaa caaggtaagt
    29341 ccagcacctc gtaagaacat attgccgtat tgtttccaaa tcgtagtgaa atcagaaatg
    29401 atgccaggtt tttcttcact atcagttgca gcaggctgat ctttgactgc ttggtccata
    29461 attttggttc gttcatcgtt agagatgcca gaaagaatct ggtttacttt ttggatatcc
    29521 gggtcgcctt tacgcatacc aacagaaatc tgaacatctt ccgggtttgt ctggaagcca
    29581 tctgcaccgc tgaattcaag catttccaag tctttgttca cactagttgc cgttacacct
    29641 tccggacgtt cgctgacata accatcgatc attccagaag ctaaagctgt tctcattgct
    29701 gagaaattat ccattgcttg ttgtttctgc acatctggta tctgatcgat cacagtatag
    29761 tggaatgtat ttaactgagc agtaatcttt gctccagaaa gatctgctaa actttttgct
    29821 tttgcatatt tgctatcttt ttgaactact actactaatt gtgattcgta ataaggatcg
    29881 gtaaaatcaa tttcttttcg tcgttcagca gtagggctca ttccggcgat gatcgcgtcg
    29941 atcttgcctg attgaagggc aggagccaga ccatcccatt tcgtttggac gatgactaat
    30001 tttttcccta aaccatctgc tactttttta gcgatttgaa catcataacc gccggcgtaa
    30061 ctattttggc cttgaattgg caaggcatta ttctcatctg tttgttgaga ccagttgaag
    30121 ggtgcatacc cagcctccat acctacgcga aactcatcgc tcggtgtttt ttcatctgca
    30181 tttgccagag atcccacact gaatagtgag aagacaaaag caaatagtac ggcaactgaa
    30241 accatatttt ttttcatttt tttctccctt tctgattata tgatacacga gcggtctaga
    30301 tgaaatgcgg taaagcttta tctgaaaaaa taaaaagcga ccgatttata gatataaaaa
    30361 ggccgctaaa attccataag gtgataaacc tctcacaaaa catagcgcaa cttagcatca
    30421 ctgctaagac agtccgcaag ctcttaactt gcgtcccaac atacactcca agcaaaaaag
    30481 tgcacatttc ggcgatcctc cctattcctg cttctccatg tttgcttcac tccacagggt
    30541 taatgattct cgcgacctct acctcattga ttgaggcatc tcgtattcag ttatttatac
    30601 aaaaaaagtt tacgatactc gagaggcttt gtcaatctat aactgctgtt ttttgaaaaa
    30661 atactgataa atatagaaat ctttactttt accgaaaaaa caatcaaaat gtgaatcatc
    30721 tatccattgc aaacgatagt ataattatac acaaaaatgt atgtaataca ataaaaaaag
    30781 aactagaaca gaaaatttcc tgtgtccagt tccatctcga tcatttaagc tgccgctttc
    30841 tttggctcac gtcctaggat cgcttgcaaa agaacaacga cgccataaat ggctaatatt
    30901 agataaatca aacttgagta ttgggtgatt ccccagcccc atgttcgata tacggctaat
    30961 gtaatggcta aaacaattgc ggcaatcacg ttgaataaag caaaagccca aagatttcca
    31021 ttttctttgc gtacaagata aaaaacagag aaataaacgc ttaacgctaa ccaggcgata
    31081 caagccagaa taacgcccca ataaataaag aatggtaaca tatcgattca cctcactttt
    31141 atacagcagt attgtaacac gttttcattc ttttgtcagt gaacattgtg ataaattgtt
    31201 caaatgccca taacactttt ttattcccat ttttctgact tttcaacaat aaatttgttc
    31261 gagtatgggc tataatggaa agtgaggtga caaaacatga aacaattaac agtagacgaa
    31321 gcagcaaaag agatattagc ttatcagcaa attacatttt tgaccggtgc aggggtatcc
    31381 acaccttctg gagtgccaga ctatcgatct ttgtcaggaa tctaccacgg gatggagcag
    31441 ccggaatact tgctgagtca tcaagcaatg gtgaatgaac cggataaatt ctatcaattt
    31501 gtcaaaaaga tttatcatcc tgaagcaaaa cccaatatca ttcatttgga aatggcacat
    31561 ctagcgaaag aaaaaaatgt ttgggttgtt tcccaaaaca tcgacggttt gcacgcaaag
    31621 gctaaaagcc cgcaactcgt caattttcat ggaagtttgt atcaatgtca ttgtcgaaaa
    31681 tgtcaacaaa cggtagactg gaaagaatat cttcacagcg acaaacatca agcatgcgga
    31741 ggacaaatac gcccagacat cgtgttatat ggtgaagggt ttcaagatgg agtcatggag
    31801 caggcggctt atgccgtcag ccaagcagag ctgatcgtca ttgttggaac gagttttcaa
    31861 gttcatccgt tttgtgatct ggtacagttt cgccagccgt cagccaaaat cttagtaatc
    31921 aatcaaacgc ctgtttattt agcggagcct tatacgttta ttcaagaaga cggagcggtt
    31981 gttttcaaaa aaatacaaga attaggagca gattatgact gaattcaaaa ccgaaaaaga
    32041 aaaaatgatt gcaggcgagc tttattttgc caatgatcct gagctggttg ccgaccgtat
    32101 gtttgcaaga agtcaaagcc aaatcatcaa ccaagcagaa tcagcggaac tgcgatccca
    32161 attgttgaaa gagacttttg gccgtacagg gaaaaaaatc tatatggagc cagttatcaa
    32221 ttttgattac ggatataata tatttgttgg ggagaatttt tatgcaaatt tcaattgtac
    32281 atttttagat gtcagcacga ttgaaatcgg tgataactgc atgttcgctc cgaatgtcca
    32341 gctttacacg gcgacgcatc cactacatcc agtgaaacgt aacagcggtc tggagtacgc
    32401 aaaacccatt aaaatcggta ataatgtttg gttaggcgga ggagttatcg tcacgccggg
    32461 tgtcactcta ggggacaacg tagtagtcgg cgcaggttcg gttgtgacca aatcttttcc
    32521 cgacaatgta gtgattgcag gaaatcctgc tcgtatcatc aagacagtag aggaagaact
    32581 aacagaagag tctcttgaaa cattacgtca taagatcgat atgatcgatc gagagttgat
    32641 tgctttatta gaaaaaagaa tggatacggt aactaaaata ggacaagtga aaaaagaaga
    32701 aaaccaagca gtttatgatg caaaaagaga gcagcaagta ttggataaag ttgttagtct
    32761 gctgaaaaat aagggatata aggaaacaat aacagatacg tatgttgatt tgatgaaaca
    32821 ctcgcgcgaa taccaaaaca aaatgaagga ggaataagtg tgatcgaagg agagacacgg
    32881 cgaaaacaaa tcatgcaaac cttggcaaat acagaaaaac cagtgagtgc tagcaagttt
    32941 gcgaaaactt ttggagtcag ccgtcagatc attgtaggcg atattgcttt actacgagct
    33001 gcaggtaaaa gtatcgtggc aacagcaaga ggttatatcc tcgaaacaga agaaaccaat
    33061 actgggcaca tcagtaaaat cgctgtccag catggaaaag atcaaacaga agaagaactg
    33121 agactaatcg ttgaaaatgg tggtgaaatc attgatgtaa ttgtggaaca tcccctttac
    33181 ggtgagttag caggaacttt gcacatcaaa acgccacaag atatcgattc ctttatgaga
    33241 cgttatcaaa aaagtaatgc tactttgtta tccgaattaa caggcggcat ccatctgcat
    33301 acgatccgct acccagaaaa gcatatgctg aaaaacatca aacaaaaact agctgaagca
    33361 ggaatcctct acgaaggata acatcgtagc tgctactttt tatcctagaa actattctat
    33421 catctctgag ataaacaccc atatggaaga aataagaaaa aataagaaaa gatgcgtatc
    33481 caatacacag aattttttgg aattgcgatt tctagatgtc tatctaaatt tcgattagtg
    33541 gagaaacata aaaaagggaa gtgcagcttt tgagcactcc ccttttttat aaagctttct
    33601 tgtagcatag tatcgaagga acctgccaat tctctactcg atcaccaata cgccttcttt
    33661 tagtgcaaat ggattttgat cgttgatgtg gtcatagaac atcacaccgt tgatatggtc
    33721 gatttcatgt tgaacgacga tcgattcata attcttcaaa cgaatcttgt gtttttcacc
    33781 attcatatca taataagaca cagtgatttt ggcatggcga acaacatatc ctggtacttc
    33841 tcgatcaaca gataaacagc cttcaccttc ccctaaacaa gcatcttgaa cagaatggct
    33901 caaaatttta ggattataca taacagtact tagagaaggt ccatctgcct ctgggtcagg
    33961 acttggtacg tgaacagcaa tgattctttt agaaatatct aattgaggag cagctaagcc
    34021 aacaccgcct cgtaagtgta gttcttcagc tttgattgga tcttggctgt tttttaagaa
    34081 ctcaagcatc tcttttccta aagagatatc ttcttcgctt aatggaagag aaacttcttt
    34141 tgcaacttcg cgtaaggtcg gatttccttc acgaatgata tcatccattg taatcatgga
    34201 atatgtaccc ctttcttaaa agactgtgtt tcaagtctat tttcgcatta tagtttacca
    34261 caaaaatgga aaagtagtag taagattgtt tgaaaactac cttttcttaa tgattaacta
    34321 cttgattttc aacccggttt caggtaaagt atagctgtgt gctctaggca catgacctgc
    34381 cacttggtta agcgaatcac caacgtttta ctcagtcgca ggggaataat cgaaagaggt
    34441 gaaagaaatg gcaatttcaa aagaacgcaa gaatgaaatc atcaacgaat acgctcgcca
    34501 tgaaggggat actggttctc cagaagtaca aatcgctgta ttaactgaag aaatcaacca
    34561 cttgaatgaa catgcacgcg tgcacaaaaa agaccatcat tcataccgcg gcctaatgaa
    34621 aaaagttggt caccgccgta acttgttagc ttacttacgt aaaactgacg ttcaacgtta
    34681 ccgcgaattg atcaaacgtt taggcttgcg tcgttaattt tagtcaaaaa gagtgggatt
    34741 cacatgaatc tcactttttt tactcgtata tcaattaagt aaaattccgt gatcaagcta
    34801 gatggactcc tactaaccaa atgaaagctg ctcaaaagac ggctttcggc agttttcatt
    34861 tgtttagtag cgtcttgctt tcacgagaag gaaaaggaga agttatgtca gaaaaacaag
    34921 tattcaaaac gacatggggc ggccgtccct tacaaatcga agtcggtcag ctagcaaaac
    34981 aagcaaacgg agccgtttta gtccgttatg gagatacagt agtcttaagt gcagctgtgg
    35041 cctctaaaga agcaaaagat acagactttt tcccattaac gatcaattat gaagaaaaaa
    35101 tgtatgcagc agggaaaatc cctggaggtt tcatcaaacg agaaggccgt ccaagtacgg
    35161 aagcaacatt gactgcccgt ttgatcgacc gtccaattcg cccgatgttt gcagaaggct
    35221 tccgtaatga agtccaagta acgaacatcg tgatgagtgt agaaacagac tgctcgccag
    35281 ctatggcagc aatgctaggt tcttctttag cactatcaat ctcagatatc ccatttgacg
    35341 gcccaatcgc aggcgtagaa gttggtcgtg taaatggcga gtacgtcttg aatccaacag
    35401 tagaacaagc agaacaaaca gatatcgaat tgactgttgc cggaacgaaa caagccatca
    35461 atatggttga aagcggcgcg aaagaagttt cagaagaaga catgcttggt gcattgctgt
    35521 ttggttttga cgctatcaaa gagttagttg ctttccaaga agaaatcgtt caagcagtag
    35581 ggaaagaaaa aatggaagtg actttactgc aagttgatga agtattgaaa aaagaaatct
    35641 ttgatgcttc ttacgctaca atgaaagcag cagtaatgac tgaagaaaaa cttgctcgcg
    35701 aagataatat cgagcaggtg aagatcgata tccgcgaagc ttatgctgag aaatttgcag
    35761 gtcatgaaga tgaagatcac ttgttgaaag aagttaaaca aatcacagaa gatcttgaaa
    35821 aagatgtcgt acgtgaattg atcaccatcg ataagatccg tcctgacggg cgtaaattgg
    35881 atgaaatccg tccattgtca tcagaggtca gcttattgcc acgtgtacat ggatcaggtt
    35941 tgttcacacg aggacaaaca caagctttat cagcttgtac actcgcacct ctaggagagc
    36001 accaaatcat tgatggttta ggggtggaag tcagcaaacg attcatccac cactacaatt
    36061 tccctcaatt ttctgttggt tctacaggac gtgcaggctc tccagggcgt cgggaaatcg
    36121 gacatggcgc attaggtgaa cgtgcgttag cacaagtaat cccttcagaa gaagaatttc
    36181 cttatacgat ccgtttagta gcagaagtat tggaatcaaa tggttcttct tcccaagcaa
    36241 gtatctgtgc tggcacatta gcattgatgg atgcaggtgt accaatcaaa gcgccagtcg
    36301 ccggtattgc gatgggactt gtttctgatg gtgaaaacta tacgatccta actgatatcc
    36361 aagggctgga agatcatctt ggcgatatgg actttaaagt tgccggtaca aaagatggta
    36421 tcactgcttt acagatggat atcaagattc aaggaatcac agaacaaatc ctaactgaag
    36481 cattaactca agcaaaacaa gcacgtatgg aaatcttaga agaattgact tcaacgattg
    36541 ctgctccacg tgaagaatta agccaatatg caccaaaaat cgaaatgatc cagatcgaac
    36601 ctgctaaaat caaagatgtt atcggtaaag gcggcgatac gatcaacgga atcatcgatg
    36661 aaactggtgt gaaaatcgat atcgatcaag atggaaaagt aagtattgct tcagctgatg
    36721 cagaaatgat caaaaaagct atcaagatca tcgaagattt gaccaaagaa gtcaaagttg
    36781 gtgaagttta tctagggaaa gttgtccgta tcgaaaaatt cggtgcattc gtcaacttga
    36841 tcaaaggaaa agatggactt gtccatattt cccaattagc aaacgatcgt gtcaataaag
    36901 tcgaagatgt tgttaaacta ggtgatgaga tccttgtgaa agtaactgaa atcgataaac
    36961 aaggacgcgt aaatctgtca agaaaagcaa tgttgaacga agatggttca acgaaaaatg
    37021 aagaaaaata atttaattca taggaataat gtacttgtga tcgtgaaggt gacaggggga
    37081 caataattat tcgaaaaaag gggactgtga caaaagccgt gtcacagtcc ttttttttcg
    37141 aataaacggt gttcaaaagc ttgcatctgc ggtaataagt ccagccaagt taaaaacatt
    37201 aggagctgtt tttatttggc tgttcttatt acttgatgca gaacagcttt tttacaacct
    37261 ctccaataaa cgatgttcaa tcagctgtcc ctcgatatta gctcaaaaaa cggcaaaata
    37321 tgaggaacta tttttccgtt tgtttctcta atactcaggt caaaccgctg atttcacaac
    37381 ctctgattca cggtgttcca gaagcagctt ctacggaaat aagctgaaca cttttgttac
    37441 aacttctttt tttctggagt ttcttggcgc atatttttgg agtccttctg aataaagcgt
    37501 aaaataaaag tgagaaatat ggataaagga gggaaaggag atgctgaatt ttttatggtc
    37561 gttatttatc ggcggtatat taggtttgat agctggcgca attttaggaa aggaagtccc
    37621 gggaggcgta tctggaaata tcattatcgg ttttttaggt agttggctcg gcgaattttt
    37681 actaggacca ctgggaccgg aactcggcgg tttttatttg ataccagcat tattgggtgc
    37741 aattttttgc cttgctcttt attcatttgt ggcaagacgt atcagagaac gtccaaagaa
    37801 agaataattt tattcaaata agttgacact tcagtgtgtt acttggataa ttagaagcaa
    37861 tttaggaaag cgggtgtcat ggataagaat atcatctatc attcaagctt acctaaagga
    37921 tatccatata acaagaaatg agggaaaaac gtggcaacaa cgcacaatac caaaaagata
    37981 tcaatgactg gcctgcttgt agcgatggga gtcgtctacg gagacatcgg gacaagtccg
    38041 ctttacgtta tgaaatcgat tgttgaagat aatggcggat tgtcaggaat caatcctgat
    38101 tttgttatcg gatcagtctc attgattttt tggacattga ctctactgac aacaatcaaa
    38161 tatgtcgtga ttgcattgaa tgcagataac catggtgaag gtggaatttt ctcactctac
    38221 acacttgtcc gaaaaggtgg gaaatatttg atcattcctg caatgatcgg aggagctgcc
    38281 ttactagcag atggggtatt gacccctgct gtgacggtaa cgactgcgat tgaaggcttg
    38341 cgaggaattc cgcagttttt tgaccggttc ggtaataacc agaacgtgat cgtcattatc
    38401 actttagtta ttatttttgt tttattcatg gtccaacgct ttggtacaga tattgtcgga
    38461 aaagcattcg gaccaatcat gttcgcttgg tttacttttt taggcgtaat gggattaatg
    38521 aacttcagtc aagactggac agttatccgt gcactgaatc cttattatgc tatccagctg
    38581 ctttttagtg cagataacaa attaggcttg ttcattcttg gaaatatttt cctggctacg
    38641 acaggtgcag aggcgcttta ttccgatctt ggacatgtcg gcaaacgaaa tattcgtctg
    38701 agctggcctt acgtcaaaat ttgtttggtt atcaattatt ttgggcaggc tgcgtggttg
    38761 ttgaatgttt atcaaaaccc tgaagcacag caaatcaaga acctgaatcc attcttccaa
    38821 atgatgcctc aaagttggat cgtgatcggc gttgcttttg caacagtcgc tgcagtcatt
    38881 gcttcgcaag cattgattac aggttcgttt acgctagttt cagaagcaat caagttgaaa
    38941 ctgctgcctc ggttaaagat catgtatcct gggaacagta tcgggcaaat gtatatccca
    39001 gcagtcaatc tgattttatg gatcgcctgt tctctagtgg ttgtcacatt ccgaacgtct
    39061 catcatatgg aagctgctta cggtctatcg atcactgtga cgatgttgat gacaacagca
    39121 ctgctttact tttatcttat ccaaaatggg cattctcgtt ggcttgcata tttagttacc
    39181 tttttcttcg gtgcgataga agtcgttttc tttatctcaa gtgttgtcaa attcttccga
    39241 ggcggttttg tagccgttct gattggtttg atcatcttag tcgtgatgtt tatttgggaa
    39301 caagggaaca ttattcgcga atcgtcaacc gaggatgtgt ctttgagaga ttatatcccg
    39361 cagcttagag aattacgtga tgatacttct ttaccaatct atcagacaaa tgtggtcttc
    39421 ttggttcctg atatggctga agataaagtt ggccgacagt tcatctattc gattttagat
    39481 aaacggccta aaagagcacg agtctattgg tttgttcatg tggaagtgac ggatgagcca
    39541 tatacgaaag aatatcaagt agatatgatg gacactgatt ttattgtcca agtgaatctg
    39601 ttcttaggtt tcagggtcca acaggaaatc aacgtatatg ttcgccaagt catccatgat
    39661 ttgatgaaac aaggccgttt gccaaaacag ccacaaagat actctctgac accagggcga
    39721 gaagttggcg atttccaatt tgtcctgatt gatgaagtag tttccaatgt gacaactcta
    39781 ggaaaatggc aacgtcaaat catgcaggca aaactggcaa tcaaaaaaat cgctacctcg
    39841 ccagaaacat ggtttgggct agagtacagc gaagtaaaac atgaaactgt tccgcttatc
    39901 attggaaata ctcggaaaac atggttgaaa gagcggttgt gaaagtagcg ctttgacctg
    39961 aactaatgtc gaatgtcagc tacttgaaca ccgtttatca gaggtcatga aaaagctgta
    40021 ctgcaacaag cagtaagaac gagcagataa aaacagcttc tcatgttttt catctgtttg
    40081 ggcttaatgc cgtagatgca agcttttgaa taccgttttt atcagaggtc atgaaaaagc
    40141 tgtttagcaa caagtaacgc aatgattttg aagtgataag gagatgtggc tgaaaaatgc
    40201 cgcatctcct tttttattcc taaatatgaa tacgcattat tttcaatctt tttgtagaaa
    40261 aagggtacaa tagaaaaaga gaaaggcggg agaatatggt tatcaatttc attattttat
    40321 ttttgatgat tgcaattact tcattttttg tagcaagtga atttgcttta gtgaaaatca
    40381 gacgatcaag attggaacaa ctagaaaagg aaaatgtaaa gaacgccaag ctagctcttc
    40441 atgttacaca ccatttagat aactatttat cagctagtca attaggaatt acgctaaccg
    40501 gtttgatcat cgggtgggtc ggtgaaggat ctgtcgcggc tttactagaa ccagtgatcg
    40561 gtaaactacc ttttagttcg gctattagca gtacaatctc tgtggcgctg ggatttattt
    40621 tagttacgta tatcgatgtg gttattggcg aactgcttcc taagagctat agtatcgtca
    40681 atacggagaa ggtcgtcttg ttcgtagtta aaccacttca ttatttttat aaagtaatgt
    40741 ttccttttat ttgggtattg aatcattcag ctgcagggct agggaagcta ttaggtgtgc
    40801 gtctcgtatc tgaaggagaa gagacattgt ctcaagaaga gctgacatta gtcgcattga
    40861 attcttatga aaaaggagag ttgaccaaag aagaatatta ttatttggaa aacgtgtttg
    40921 aatttgatga taccttggca aaagatatac aagtagaccg tacgtccatg caagtattcg
    40981 aagcttcaga aaccgtcaaa gaagcgataa aagcttcgat tgaacaagga catactcgct
    41041 atccggttat cttagaatca aaagacaatg ttttaggtta tgtcaccttg ccagatttga
    41101 tcaagcaatc ttataaagat gaccagctga cagtagaaca gttgattgaa gagccgatcg
    41161 taacaactga aacgatccct ataaaaaaat tattgacgat catgcgaaaa aagggtaagc
    41221 atattgctat cttaaaagat gaatacggag ggacaagcgg cttagtgacg attgaagata
    41281 ttttagaaga aatcgtcggg gaaatccgag atgagacaga tttggacgaa gcattgatag
    41341 cggaacagtc agatggctct tatatcatct ccggcaaact aacattagat gatttccagc
    41401 gttattttca tgtagagatt ccagagtttg aagaaacgaa ttttactaca cttgcgggct
    41461 ttgcttctag ccgatataaa gaaataaaag caggaacaat cattgagatt gcatcattcc
    41521 gtttcacggt attagaatac cagcatgcac atatcgatta tttcaaagta gagtccacgg
    41581 aaagaaaaac agaataaaag aaaaaaggct gtgacatttt ttgtcacagc ctttttgtta
    41641 tctaaaaaac ctgttttcgg taaacgaaga agatattttc aataaaagtt ttcattttta
    41701 gccgcaacga tttcttttaa tcaaggctat cttgcttctg catgttctaa tgtgaaatgt
    41761 tcctcttcac ccatgtgttt caattgtttc atcgaacgca tataagagta aagcaacacg
    41821 gcaacagaac cgatccaagc taaaggagaa gctgctgctg ctcctgggta tccccacatt
    41881 cctgtaagaa cgatagctgc aaaagaacgc ataagcaatt ccatcatacc ggcgatcgta
    41941 gggatcttgc tttgtccaag cccttgaagt gtgtagcgta aaatgaacag gactgctaag
    42001 atccagtaaa gagacccatt gatattgaag taggtttgtg ctaattcaaa tacacgtgtt
    42061 tctgatgggt taacaaacaa ttgaacaaag aaatgaccga agaaaatgac gatagcacct
    42121 gctaagagac taaatccgac actcatcagt aaagtttgac gtaccccttg caagattcgg
    42181 ccatattctt tcgcaccata gttttgtgca gtgaaagttg ccattgcaat accaaatgac
    42241 atcatcggct gatttgcaaa ttggtcgata cgaccagcag cggcttgagc agctacgaca
    42301 tctgtcccta gactatttag tgctgcttgt aacacgatag caccaacagc gatgatcgaa
    42361 gattgaaacg ccattggcaa agcagcattt aggtgtacag caaactcttc tttttcaaat
    42421 ttgaaatctt ttttgcgtaa ctgtaacaaa ggcatttttt tcttgatata cactagacac
    42481 agtacgctgg aagaaacttg tgcgattact gtagcaatag ctgcaccttc cacacccatg
    42541 ccaaaataga taataaagat caaatcaagt acgacgttga tgatgaccgc aatgatcaga
    42601 aagaatagtg gtgtgcggct gtcaccaagt gcgcgaatga cattagaaag caaattgaaa
    42661 ctgactgatg caaagatccc taataaaata attgaaataa atgtttgtgc ttgatcaagt
    42721 atttcaggag gagtttgcat caattgaagc atcggacgaa taaacaggag acttaggaca
    42781 gttaaaatca cagtgacgac aagactaatg acaacactag cagcaaaact tttcttcaga
    42841 ccccgataat cttttgcacc gtaacgttgt gcggtaataa tggctaaacc agctgttaaa
    42901 ccttgggcaa agccaatgat caaaaatgtc aggcttccag ttgcaccaac cgctgccagc
    42961 gcatttttac ctagcgtctg acccacgatg atcatatcga tcatattgta gaactgctga
    43021 aaaacatttc cgatcagtaa aggaacagtg aacatcaaaa tcagtttagc ggggcttcct
    43081 tttgttaaat cgcgcatctt ttaaactcct tatatcttta aaatttagtt agtagtatat
    43141 agcgtttatc ctgagaatac caattagaaa tcaatgataa tttatgaaac tccataaaaa
    43201 gaagccaaga attgagtcaa agaatcaaca acttggcctg gtctgtaaaa taagggacat
    43261 ccacgtttat ctatcacctt aatattgtct aatctcactt ggtaaaagga tcgtttctct
    43321 cgtaccgtca tcataggcgt atacttgagc aggaaattgg ttcgaatagg caaacggcaa
    43381 atcaatatcc atttccacat tgcttcctgc ttgagaaaaa tgcatcgtca cttttccacg
    43441 attttcgatc aaatcaaagg ttaatatatt ttctaatgga atgactccct taagatctag
    43501 gtcaataata aaccatatac tgtcaatcaa ttcatctggc aaacttgata cgacaccaac
    43561 tgaagcgtaa cggttgcggc tgctatcaaa actttcaaac atctgtttaa cctccttcga
    43621 atcaacgaat gttttatttt tataattaat cctaggataa actagaaata cttacttcaa
    43681 cgagggttca ttctaataaa gaatcaggaa aaatgcaaca ataattgaat actttttttc
    43741 aaagtataaa tttttataaa ggtacgataa aagtatgcga ggaaaagctt ggtaaattag
    43801 gatttttcgt ttgtaaagcc atttacaaaa gtggaagccc catatgaaaa taaacgatta
    43861 gaaataaaaa aggcgtgtta aaagctgctg acccgccgct tctaacacca cctgattgtt
    43921 tcagtattat tcttctgttg aaccgatcac ttctggttcg ccaccttcac ctgtgctttc
    43981 ttcttcttca ctttgagcga ctacagcagc gatttgctct tcactgtcgg tcacgaattt
    44041 gtaatcgtcg ttttttggaa gatcggctac tgtaatagaa tcacctaatt ccaagtttgt
    44101 cacgtcaact tcaattcgtt caggcaattt gtctggtgta gcagatacga ctactgtata
    44161 caagttttga gctaacactc caccagattt agcacccact gattcgccaa caagaacgac
    44221 ttctgtttcg acttctgttt cttccgacat gtttacagat agaaattctg catgttcgaa
    44281 atgattggta aaagtgttcg tttggatctt gtgaaccaat gtattgactt tttgtccatc
    44341 gatgtccatc gtgatgactg tgttcgctcc atgttcacgc aacaatttgc ttagtgtcaa
    44401 accatcgaat gagatagggg tactttctac tttataccca taaacaacag ctggaacttt
    44461 cccttcgtga cgaagtttgt ttcttaatga acggggacgt acttctcttt tacttacttc
    44521 taatgatact gacataacca aaattcctcc ttaaaatgtg actgtctgct ttggtaacgg
    44581 acagatttta tttaagtgaa ttctaaaggc taatctcgtg cacatcatcg aaggcagggc
    44641 gcttaaaaaa gcgcaaaagg agagatgcgc gcatctcccc ttagaaagtc aggattcaac
    44701 ttttctccac caggtcatta cgctgtgtat gagaaatcca attcacttta ctatttaacc
    44761 gtaacgtgaa ctgttaatta agtcaaggaa taactatgat gatgtatttg caagcaagaa
    44821 tattcaaggt ataattaatt agtagtaata agaaaggaaa cagcatgcgt ttagataaat
    44881 taatagaaga aaaattagaa acgacacgca aggaaatgaa gcggctgttt gctttgaagc
    44941 aagtgatgat agatggcaca gtcgaatttc gacaaaatcg aaatgtagat agtttactgc
    45001 atcaaatcga agtagcaggt gaacacttag aaacaaatga gtcctattat cttctcaata
    45061 aacctgaaaa agctgttaca gctgtatcag atccagaaaa gacgacagta atcgatctga
    45121 ttgcaagtgc cgatcgtttc caaccattat atccagtggg aagactagat agagatacaa
    45181 caggactatt gctgatcact tcgaatggac aattgggata tgaaatgcta cttccagaaa
    45241 aaaaggtttc aaagacttat caagtagttg tgaacgaaag ggtgacaccg gcagacgtaa
    45301 cagcatttga aaatggaatc gtattcgctg atgggtatca atgtaagccg gctgatttaa
    45361 aaatactcaa agcagacaag caccaaagta cggttttatt gacgatccaa gaaggaaaat
    45421 tccatcaagt caaaaagatg ttcttagccc aaggaaagaa agtgaccgca ttgaaacggc
    45481 taagcatggg accattggaa ttagatgaac agctaggagt aggcgagtat cgatcgttga
    45541 cattagcaga gatgaaaaag ttaagtattt attttaaata ggaggtcaaa aagatgaaat
    45601 ataaaacttt attgtttgat gtagatgata cattgttaga ttttcaatta actgaagcca
    45661 atgctttaag tgctttgttt gcagaacaag gaatcacatt gacaccagaa atcgaaacca
    45721 gctataaggc tttgaatcat catttatggc aggagtttga aaaaggcaga ctatcacgag
    45781 aagaaataat aggcagtcgt tttgggttac ttttccagca atttgggaaa acagtagata
    45841 gtgatcaaat ggaaaaacgc taccgccact acttgaacca aggtcatgat cttgttccag
    45901 gaagtctaga aatcctgaaa aaggtccatc cgcatgctga tctttatatt gtgacgaacg
    45961 gtgtgtcgca cacgcaacat cggcgattaa cagatgcagg aatgcttgat tacttcaagg
    46021 atatctttgt ctcagaagct gtcggagcgc aaaagccgat gaaagaattt tttgatcatg
    46081 tcttttccca cattcctaat tttgataaaa agcaaactgt gatcattggt gattctttga
    46141 cttctgatat caaaggagga aatatggcag ggatcgatac gatttggttc aatagaaata
    46201 aattaccaga aatcgaagaa atccagccta cttatcgaat cgattcatta gaggaacttt
    46261 attcattatt ggaaattgaa aaggattaat tttcagaaaa atatagcatt ttcgtgtttt
    46321 ttcgctataa tagattagaa tgaaaaaacg gatacgaaag aggttttagt caatgacaaa
    46381 aagcaagcca attatcattg gtgtgacggg tggatccgga agcggaaaga caagtgtcag
    46441 ccgtgcgatt ttcaacaact tcccggacca ttccatcatg atgcttgaac aagattctta
    46501 ttacaaggat cagagccatt tgagttttga agaacgattg aacacaaatt acgatcaccc
    46561 atttgccttt gataatgatc tgctgattca gcatgtaggc gacttactga actataaagc
    46621 cattgaaaaa cccgtgtatg actatgtagc gcatacaaga agtcaggcaa cgatcattca
    46681 ggaaccaaaa gaagtgatca ttctagaagg aatcctgatt ttagaagacg aacgtctgcg
    46741 tgacttgatg gatatcaaag tatacgtaga tacagatgat gacattcgta tcattcgtcg
    46801 tatcaaacgg gatatggaag aacggggacg cacattggac tccgtgatcg aacaatattt
    46861 gacagttgtt aaaccgatgt atcatcaatt tatcgaacca acgaagcgct atgcggatat
    46921 cattgttcca gaaggcggag aaaaccacgt tgctattgat ttgattacaa caaaagtagc
    46981 tagtttctta aaccataaat agtgcgcagc gaaacagttg aacggactgc gaccgtttga
    47041 aaagcaagga ttctgctttt tgaacagtca ccagccaact gtttttttat ttataacaag
    47101 aggttgtgaa aacagcatct tgacctaagt attaaaaaga agaaacggaa aatagctgtt
    47161 tatattttac cgttttttga gctaataccg aaggtcagct gattgagcac cgtgaagtag
    47221 aggttgtaaa aaagctgtct ttgcatcaag cgataagagc atccaaataa aacagctcct
    47281 cacgtttttc gttgggttgg gcttattatc gtagatgcaa gcttttgaac accgtttaac
    47341 gagaggttgt aacagaagtg gtcagatgca ataaataaga agaaacttac gaaaattatt
    47401 tctcgaattt gtgtgagatt tcgacttatt tttttgaaga agctcttttg tcgcaccccc
//
LOCUS       DAHHZJ010000006        47393 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.006, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000006 DAHHZJ010000000
VERSION     DAHHZJ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.006"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(87..1238)
                     /gene="fucO"
                     /locus_tag="NBL02_000234"
     CDS             complement(87..1238)
                     /gene="fucO"
                     /locus_tag="NBL02_000234"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCD2570950.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(1320..1631)
                     /gene="rhaM"
                     /locus_tag="NBL02_000235"
     CDS             complement(1320..1631)
                     /gene="rhaM"
                     /locus_tag="NBL02_000235"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCD2570951.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(1633..2478)
                     /gene="rhaD"
                     /locus_tag="NBL02_000236"
     CDS             complement(1633..2478)
                     /gene="rhaD"
                     /locus_tag="NBL02_000236"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HCD2570952.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(2492..3748)
                     /gene="rhaA"
                     /locus_tag="NBL02_000237"
     CDS             complement(2492..3748)
                     /gene="rhaA"
                     /locus_tag="NBL02_000237"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCD2570953.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(3745..5205)
                     /gene="rhaB"
                     /locus_tag="NBL02_000238"
     CDS             complement(3745..5205)
                     /gene="rhaB"
                     /locus_tag="NBL02_000238"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCD2570954.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            5358..6227
                     /locus_tag="NBL02_000239"
     CDS             5358..6227
                     /locus_tag="NBL02_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HCD2570955.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(6353..7252)
                     /locus_tag="NBL02_000240"
     CDS             complement(6353..7252)
                     /locus_tag="NBL02_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD2570956.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(7417..8241)
                     /locus_tag="NBL02_000241"
     CDS             complement(7417..8241)
                     /locus_tag="NBL02_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCD2570957.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(8252..9547)
                     /locus_tag="NBL02_000242"
     CDS             complement(8252..9547)
                     /locus_tag="NBL02_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HCD2570958.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(9563..10042)
                     /locus_tag="NBL02_000243"
     CDS             complement(9563..10042)
                     /locus_tag="NBL02_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCD2570959.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(10048..11076)
                     /locus_tag="NBL02_000244"
     CDS             complement(10048..11076)
                     /locus_tag="NBL02_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HCD2570960.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(11093..12199)
                     /locus_tag="NBL02_000245"
     CDS             complement(11093..12199)
                     /locus_tag="NBL02_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HCD2570961.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(12428..15025)
                     /gene="adhE"
                     /locus_tag="NBL02_000246"
                     /gene_synonym="adhC"
     CDS             complement(12428..15025)
                     /gene="adhE"
                     /locus_tag="NBL02_000246"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCD2570962.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(15330..15710)
                     /gene="yajC"
                     /locus_tag="NBL02_000247"
     CDS             complement(15330..15710)
                     /gene="yajC"
                     /locus_tag="NBL02_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCD2570963.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(15807..16952)
                     /gene="tgt"
                     /locus_tag="NBL02_000248"
     CDS             complement(15807..16952)
                     /gene="tgt"
                     /locus_tag="NBL02_000248"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCD2570964.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            17170..17868
                     /locus_tag="NBL02_000249"
     CDS             17170..17868
                     /locus_tag="NBL02_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HCD2570965.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(18036..20675)
                     /locus_tag="NBL02_000250"
     CDS             complement(18036..20675)
                     /locus_tag="NBL02_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCD2570966.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(21294..22325)
                     /gene="queA"
                     /locus_tag="NBL02_000251"
     CDS             complement(21294..22325)
                     /gene="queA"
                     /locus_tag="NBL02_000251"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCD2570967.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            22518..23693
                     /locus_tag="NBL02_000252"
     CDS             22518..23693
                     /locus_tag="NBL02_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HCD2570968.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            23697..24338
                     /locus_tag="NBL02_000253"
     CDS             23697..24338
                     /locus_tag="NBL02_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2570969.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            24335..25252
                     /locus_tag="NBL02_000254"
     CDS             24335..25252
                     /locus_tag="NBL02_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HCD2570970.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            25249..25920
                     /locus_tag="NBL02_000255"
     CDS             25249..25920
                     /locus_tag="NBL02_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2570971.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(26461..26949)
                     /locus_tag="NBL02_000256"
     CDS             complement(26461..26949)
                     /locus_tag="NBL02_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HCD2570972.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(27150..27416)
                     /locus_tag="NBL02_000257"
     CDS             complement(27150..27416)
                     /locus_tag="NBL02_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCD2570973.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(27416..27679)
                     /locus_tag="NBL02_000258"
     CDS             complement(27416..27679)
                     /locus_tag="NBL02_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCD2570974.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            28155..28340
                     /locus_tag="NBL02_000259"
     CDS             28155..28340
                     /locus_tag="NBL02_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCD2570975.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(28482..30305)
                     /locus_tag="NBL02_000260"
     CDS             complement(28482..30305)
                     /locus_tag="NBL02_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCD2570976.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(30903..33608)
                     /locus_tag="NBL02_000261"
     CDS             complement(30903..33608)
                     /locus_tag="NBL02_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HCD2570977.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(33914..34861)
                     /locus_tag="NBL02_000262"
     CDS             complement(33914..34861)
                     /locus_tag="NBL02_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HCD2570978.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLEASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            35051..35320
                     /gene="rpsN"
                     /locus_tag="NBL02_000263"
     CDS             35051..35320
                     /gene="rpsN"
                     /locus_tag="NBL02_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCD2570979.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(35496..35858)
                     /locus_tag="NBL02_000264"
     CDS             complement(35496..35858)
                     /locus_tag="NBL02_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCD2570980.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(35869..36990)
                     /locus_tag="NBL02_000265"
     CDS             complement(35869..36990)
                     /locus_tag="NBL02_000265"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCD2570981.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(37006..37356)
                     /locus_tag="NBL02_000266"
     CDS             complement(37006..37356)
                     /locus_tag="NBL02_000266"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCD2570982.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(37486..38997)
                     /locus_tag="NBL02_000267"
     CDS             complement(37486..38997)
                     /locus_tag="NBL02_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCD2570983.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(39319..40671)
                     /locus_tag="NBL02_000268"
     CDS             complement(39319..40671)
                     /locus_tag="NBL02_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCD2570984.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(40824..41900)
                     /locus_tag="NBL02_000269"
     CDS             complement(40824..41900)
                     /locus_tag="NBL02_000269"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCD2570985.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            42096..43841
                     /locus_tag="NBL02_000270"
     CDS             42096..43841
                     /locus_tag="NBL02_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HCD2570986.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(44121..44558)
                     /locus_tag="NBL02_000271"
     CDS             complement(44121..44558)
                     /locus_tag="NBL02_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCD2570987.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(44542..45915)
                     /locus_tag="NBL02_000272"
     CDS             complement(44542..45915)
                     /locus_tag="NBL02_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2570988.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(46237..46725)
                     /locus_tag="NBL02_000273"
     CDS             complement(46237..46725)
                     /locus_tag="NBL02_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD2570989.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(46747..47349)
                     /locus_tag="NBL02_000274"
     CDS             complement(46747..47349)
                     /locus_tag="NBL02_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCD2570990.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        13476 a        10309 c         8544 g        15064 t
ORIGIN      
        1 aaataccatc aatttaaaag agatatccag atataaacta ccatttctct ttacaggcaa
       61 tttaatgttg aagatccgct tcgtttttac atcaaacttt gatataaagc aatgatttct
      121 tctactgtcg tttctcttgg atttcctggt gtgcatacat ctcgcaaagc atcttctgca
      181 attgctggaa tatctgcttc ttttacacct aattcagaaa tggtttctgg gattccaacc
      241 gctttgctca accgatcgat ctcaccacaa gccgcttctc ttacatcttc taaagacatt
      301 tcagctgctc ctttaatccc tagaacaaga gcgatctcac ggtacttttc tccagtatat
      361 tctttgttat atttcatgac agttggcaaa agtgctgcac acgctacgcc atgaggaatg
      421 ttgtaccatg cacttaacgg gtgagccatc ccgtgtacca gtcctaaacc gacatttgag
      481 aagcccattc ccgcaatata ctgtcctaat gccatttttt ctcgcccatt ttgatcgcca
      541 tctacagaag cttccaaaga atggccgatg atttcgattg cttttaaatg cagcatatca
      601 gtcatttccc aagcgccttt tgtgataaac ccttcaatag catgcgtcat agcatccata
      661 cctgttgatg cagccaattt tttcggcatt cccatcatca tatcgctgtc gatgaacgcg
      721 acgattggga tatcatgagg atcaacacaa acaaattttc ggtgatttac ttcatctgtg
      781 atgacgtagt tgatcgttac ttctgctgct gttccagaag tcgttggtat agctaatata
      841 ggtaaacatg gatttttcgt atcagcaaca ccttctaaac taatgacgtc tgaaaactct
      901 ggattcgtca aaataatccc aatggctttt gccgtatcga ttggcgaacc tccaccgatt
      961 gcgatcaaac agtctgcctc tgcctcttta acaaaagcca ctccatcttt tacattttgg
     1021 atcgttggat taggtacgat cccgtcgtat aaagcataag cgattgctgc tttatctaga
     1081 agttctgtta cttttgttgc tataccgtgc tcgatcaatc ctttgtcagt aatgacagct
     1141 gctttgccga atcctcgctg ctgaaattca gggacgatcg actcaatcgc tccttttcca
     1201 aaatacgatg tttcatttaa aatcattcga ttactcattt tctattcctc ctaatttgta
     1261 ttatttttca caataaaaac gaaagaaccc caagcaaccg gttgcccagt tgcttgttgc
     1321 tacagttcaa agactggcat caattcccaa ctgactggag aggcatctgg atttgtctcc
     1381 atgatatctt ccatgtactt ccaccatttt tgattgatag atgtatttgc cattttttcc
     1441 cataactttt catcttcgat cactaagtaa ccaaataaca aacttgtact cggatcaaga
     1501 tagatcgaat agtcgatgac cccgtgttca cgaagtgctg tacgcatttc tggccaaagt
     1561 tcatcatgtc tttttttgta ttcttcttgt ttttctgggt aaactttcat gcaaaaagct
     1621 tttttcacca tgtcagacac ctgctttcgt atgaacttcc tccaaataac ctgcttttgg
     1681 agtgacacca aacgcatctg ctaaatgcca caggttttcg tccgtgattg tttgcaagat
     1741 tggtccttgc gctttgacgt acgtatagac ttctgctgcc ttttctgctg tctcgatcaa
     1801 accaaagact tcatccatgt cttttcctga cccgtagatt ccatgttgtg gccataaaac
     1861 caatcgagtt tctttcattt tttcagctgt tgcttcccca atctcatttg ttcccggaac
     1921 catccacgga atgatggaaa ttccttctgg aaaaacaact aagcattccg tacacatttg
     1981 ccatagcgta cgactaaagg aacgttcgtc taattcatgc gtaaaactca tcgctaatag
     2041 atgactagca tggcaatgca taacgatccg attatctggg tctacttcta aacgggcgat
     2101 atgactcatg aaatgacttg gcaactcgct tgtaggaagg cattcgtttt ccagccccca
     2161 caaaagttcc agtgttttgc catcttcagc aactcgaacg attccaagat tctcggcagg
     2221 ttcgctcgct acatttttaa aatatcttcc tgaaccagtt acaataaaat agcatcctgc
     2281 taaacggctg gcatcaaaaa tcatcgagat gcggcggatc actttttcag gatccaagaa
     2341 cggtgtgatc tcttcttctt ttagtaaata agaaatattc ccgccatttc tttcatccca
     2401 tcccaagcga taaagattcg acgtcgtttc gatcatttct ttcacataag gcgcttgtaa
     2461 aatatctatt tttttcaata tagccacact cctattttct taattccaac acgtcttttt
     2521 gatattcctg gacttcttcc agccaatcca aaccaactgg aacttgattt ttgtaacaga
     2581 aataattcca tacatcggca tacgggaaat ctttcaattc ttccgtataa gccattcttg
     2641 aagtaaagtc aaaagccagc tccatttctt tcaatcgctc aactggttcc agcatagctt
     2701 tcagcagtgc tttttgtgta tttctcgtac cgatgaccca agcagcgata cggttgatcg
     2761 ttgcatcaaa gaaatccaga ccgatatttg ttttttccaa caattcgtta cggactaatt
     2821 ctttagcgat ttcctgcaat tcatcatcca tcaaaaccac atggtctgaa tcccaacgca
     2881 ctggacggga aacatgcaac atgattcctt tgctgaataa agctaatgaa gataatttat
     2941 tggaaatcac ttctgttgga tgaaaatgtc cagcatctag acaaacaagt ttgtcacgag
     3001 tcaacccgta acccatataa aattcatggg aaccgactgt ataagcttca gcgcctaaac
     3061 caaatagttt gctttctact gcatcttgta cataccgttc atctaactct tctgtaaaaa
     3121 tttcatcgag tgattccatt aaacgttccc gtggggtaaa acgatcaatc ggattatctt
     3181 tcatcccatc aggtacccaa aagttattga tacttgtttg cccagtttct ttcccaaagt
     3241 actccgcgat tttgcggctt cttttcccgt gttcgatcca gtattcacga acttctttat
     3301 ctggatgagc caatgtaaag ccgtctttca tcattggatg cgaaaagaaa gtcggattga
     3361 agtcaagacc gatttcttct ttttttgccc actctaccca tttttcaaaa tgtttcggct
     3421 caatttcatt tagatcgaca gattcttctg tatccaaata gatagcatga aggttcaatt
     3481 tgtgtttgcc cggtatcaac gaataagcct tctccagatc ttgtctaagc tcttcaggtg
     3541 ttcgtgcggc tcctggaaaa ttccctgtcg ccatgattcc accggtcaat tcgccatccg
     3601 gtgtcaagaa acctttgaca tcatctcctt gccagcaatg catagaaatt ttgatgtcct
     3661 gtatcttttc tagtacagca tctgtatcta caccgatagt tgcatatttt tcttttgctt
     3721 gttcgtaatt tctttcaatc gtgttcatac gattactcct ttgattttat tcacgtacca
     3781 ttccatcgtg ttttgcttat tttcatctgg gtaaaaagtt tcacatggaa acgactgttt
     3841 gattacattt cttgcttgtt ctatattcga aaactgacct gtactgagca tttgcatggc
     3901 aacattcccg attgctgtgg cttcactagg tcctgcttct acaatgattc cggcataatc
     3961 tgctgtcaat tgattcagta gacgattatt cgatccgccc cctacgatgt gcagcttttc
     4021 caatttcgtt ccagttaagt gttctacttt gatcagctct tgtgcgtaac aaagagcaag
     4081 attatcatac acacaacgag cgatagctcc cgatgagaca gggactggct gctctgtttc
     4141 aaaacagtat tgctgaatct cttctatcat atttggggga ttcaaaaatc gagggtcatt
     4201 gatttccatc acttgctgca gcggtttttc ttttgcagca agctctgcca attccgcata
     4261 actgtactcg tactcttgca ttcgggcaac ttcctggatc aaccacatcc ccatgatgtt
     4321 tttcaaaaaa cgcaccgtgt tatgggcacc ccattcattc gtataatttt cagaaaaagc
     4381 attgcgagaa acattggcga tctttgtttc aatccctaaa agcgaccatg tcccactgct
     4441 gatataaccc caacgttctc cttgacctgg cgttcctaat acagctgacg ccgtatcatg
     4501 acttgctact gtgataaagg tcacatctgg caaatcatat tttgaaaatt gttctttttg
     4561 cagatctcct agtatcgttc ccggctctgt caatggtgga aataattcct tcgagacacc
     4621 gattttttca agaagttcta tttcccattc ttttgtccct gcattcagca gctgcatagt
     4681 agatgcatta gttttctccg tcaccatgtt accagtaaag acatacccta aataatcagg
     4741 tatcagtaac aatttatgtg cttttgccaa attttctttt tcttcagcaa acaattggaa
     4801 caaggtattg aaaggctgga tctgaatccc tgtctgctca tacaacttat ctagagaata
     4861 gatttcagaa aactgttcta ctgctccatt ggtacgcttg tcccgataag caatcggatc
     4921 atttatccga tccccttgtt catcaatcag acagtaatcg actgcccatg tatcgatccc
     4981 cgcaaaaagg cgatcaatcc ctgtctgctt taatttttca aatccaatca aaatctcttt
     5041 gataagataa tcgatcttcc agcgatcgtg accgtctttg ttccgaaagc cgtttttgaa
     5101 ccggtgtact tcttccaaga tcaaagaacc ttttgcatcc ctcgtacttt tcattagtcg
     5161 tccgcttgat gcaccgatat cgatggcaac aaatgttgga ttcatttctc cacctctttt
     5221 actttattgt tgattagcac tttattaata aagcgttatc atttattgac aatgctatca
     5281 taatacagaa tcttatattt tgcttcacac tattttggta tataattgca ttatcttgac
     5341 aagttgggag tgtttacatg gattactggg aaaaagccaa ccactcatgg tcggaagact
     5401 caaaaagagt gatcttgacg cctacacaaa gaagcaggaa tttatttttt tatatccaag
     5461 aaatcggtta cttcaaagca ccaatccctt actatactga acgagcacat ttaccttctt
     5521 acttgataaa gtttactttg tctggcaaag gttcgcttac atataatgat aatcagcaac
     5581 agctaaaagc tggagactta ttttttattg actgccaaga atatcagcgt tatcaaacga
     5641 tcagcgaaga accatgggaa atggattgga tccatttgaa tggtcagaac gtccaatcat
     5701 tttatcagga gttcacaaaa gacggccata acaccttcca ttcaacagga gatccatcag
     5761 aaaacaagat ccaccatttg atcacagaat tgatccacat gcaacaaggc acgaatgccc
     5821 gtaccgattt ccagtcctct gtagctatcc acgagttgct gaatgaactg attttgcaaa
     5881 agtatcagct agatttcgaa gaagaggata ttcccgccta cgtttacaaa ctgaagcaat
     5941 acttagacga acatttcaaa gaaacgatta cacttgatca attagaacaa caattcttat
     6001 tgaataaata tcagctgagc aaagattttt ccaaatttat cggttctcca ccaatcgatt
     6061 atttgatcag caaaaaactt tcttacgcta aagaccttct tcggtataca gaatttacgg
     6121 ttcgaatgat ttccgaagaa atagggattg agaattttgc ttactttagc cgcctgttca
     6181 aagcaaaaac aggactatcg ccgagagact ttcgtaaatt cagctgattc atataatatg
     6241 tacgtcattt tttcaaaata ttttgaaata tatagataaa aataaaataa ccgcagtttc
     6301 tgcttctatg taaacaacag cagctactgc ggttattttt tagtgatcat tctcattgtt
     6361 catcttcaaa atgttcataa tgaaccaact gtttctgtgt atcacgtttc aaaaattctt
     6421 taggcgttat gccgaactct ctcttgaatg ctttcagaaa atgcggataa gaagaaaaac
     6481 cacttttact atatacagca ctagagctgc ggtattctcg caacaagtgc ttggcataga
     6541 tcagcttttt cttcattaca tactgatgaa acgtaaatcc agtgtacttt ttaaattctc
     6601 gtgtgatatg gtatttgctg acaaaaaact gcttttccat ttcctctaaa gaaagcggat
     6661 gagataaatt ttttgagata tattgtaaca tttgttccac acgctcatta ggaactacag
     6721 cattctgttc ttgcgcactc tcttcttttt gtacaacttg attgagaaaa atcaagtatt
     6781 ctaccagagc ttgttcatat tttatgtctt caccgtaaat ttttttatca ggaggatggt
     6841 cgataaatga taatttttgc ttcaatactt cctgttctat ttgcaataca cgactcctct
     6901 tctctcccat tggttgaaaa caagccgata gattagacca tggcgtggaa cgactttcta
     6961 gaaaagaagg ggtgacaaag atatagacac gctcgaatac atctgaagat tggcggacga
     7021 tacgatgcaa atcattggaa tggatgagca gcacacttcc tgctttgatc gtaaattgtc
     7081 gtccgttcaa ataaaaagtc gcttctccct ttaatgtagc atggatctca tagaaatcat
     7141 gatgatgata gatcgtttta ttttggctga cgacatcttt gacatgatag atttcatact
     7201 gatcagaaac catcgtattg acttgttcac cgctgatttt cttttggagc atgtgccgca
     7261 cctccttttg ttcacttatg cacaatttta ttataccacg ttttttgtaa tcaaaaagag
     7321 ctgcttttat atactcttat aaaaaagaag agacgtggca tcttctagcc ccgccccctc
     7381 cattctaaat attagttaac caacgtcttc tttacattat tcaagaagac ttcttccata
     7441 acgaatccca tcatcttttg tttcttcaag aaccacatac aggtacggac gattcttcat
     7501 gatgatttct ttgatttttg tgtactcgat cacaccgtct cccaaattta ccggcacgat
     7561 tttttcctgt tcgacacgaa aatctttcaa atgaaccgca catatctttt caccaaaacg
     7621 ttcaaaagct tcttctacca attggacttg ttcttgataa tcactacttg tgatcaaatt
     7681 ggtcggatct aaaatgattc ctagatagtc cgaatctact gcttggatca gctgctctac
     7741 ccttgctaaa gaatacaccg gatgattcaa acctggttct atccccacca tcatttggta
     7801 tttttctcca gcttttacca agcgacggat aacggacaca gcttcagcaa atgcttcatc
     7861 tgtgaaattt tcttccgtat attggatttc cggcaaaaca catccagttt ccgaagcaac
     7921 catcgacgca ccaaaggaag aagcatagcg aagatactgc tcgaactttg tcagcaactc
     7981 ttgtcgtatc gttaaatctg gatgaatcat attgatatag caacttaaaa ttcctacggt
     8041 cacttcttgc ttttctaaaa ctcgtttgac ataatttccc atcccagaat tgatctcatt
     8101 tgcgccagaa ggtatttcag gaaacgaaag acctaaggcg agctgcagtg tatgaatccc
     8161 ttgttctttt gttttttctg caagatcttc tatcgtttgg acttcggaaa gatcatgtcc
     8221 tctaattgta atgttcaaca taactattct cctaactcag catattgggt agccacatga
     8281 cgatttgcgg gaagtaagta atcaaaaaca agacgactaa gataacccca taaaaaggaa
     8341 gaagcggttt cagcattgcc tctgctttta cgcctcctac acttgcaccg acatatagtc
     8401 ctgtacctac tggcggcgtg atggagccta cacacagatt catgattgaa aacaacccgt
     8461 aatgtacagg atcaacccct acacttgtga cgattggtaa aaatattggt gtaaaaatca
     8521 agatagcagg cgccacatcc ataaacatac ccaccagtaa taacacaaga ttgactaata
     8581 acagaataat aaatttatta tccgttacgc ccataatcaa cgaactaatt gcctgaggaa
     8641 taccggtaaa cgccatggcg aaagacatca tggaagaagt agccaataag aacatgatcg
     8701 tccctgacat tgctactgat tgaaacaaca tattaggtat atcttttagt tttaccgttt
     8761 tatagtaaaa catagcaatc aacagggagt aaatgacagc tatcgctgaa gcttctactg
     8821 ctgtaaatat ccccgtcaag ataccaccga taatgatgac aatcaataga aggctaggaa
     8881 tcgcctcttt tattattttt ccagcatgag ccatctgtcc tttttcgaca acagggtatt
     8941 tattcttttt agcaacgatg aatgctacta gcatgattcc tagcgcccac aaagcgccta
     9001 cgagataacc tcccataaac aaagcagaga tcgaagcacc accagcaaca agtgaataca
     9061 tgataaaagc agtacttgga ggaatgacca tacctgtcgg agctgacgcg atattgaccg
     9121 ctgtcgcaaa cttccggtca tatccttctt ccacttgctg agggatcaac actccgccaa
     9181 tcgcggtaga agcggcaatg gccgagctag agatggaacc aaataaagca ttccctaaca
     9241 cattcgtatg agccaaagcc cccggcacgc gcccgacgaa caacatcgca aaatccacta
     9301 gtttcttagc tatccctcca ttattcatga taatgcccga gagaacgaaa aacggaatag
     9361 cgatcagcga aaaactattg atacttccca ccattttttg agctgaacta aaagcagaaa
     9421 catcaaacgg tataactaac aataaagtca tcatcgatga gaatgcaata cttatagcaa
     9481 tcggcatccc taatacaagc aacacaacaa atacaaaaaa taacactaat ccggcttcta
     9541 ttgccatttt attcctccat ttttatattt tttcttgaat tgctggcttg ttgcttaatg
     9601 tatcgattag attgatcagg ctatagataa tgatgagtac ccccgataca ggtactgaca
     9661 gataaacata tcccatagga atacttaaag aaggcgagat ctgccccatt gtcaatgtga
     9721 cagcattcca tccgccatac accatgatca tcgcagcaaa taccagaaat aaactttgaa
     9781 caacaacttc taaaatttta ctttttttgc cagttaattt ttcacttaaa accgttacgg
     9841 ctaaatgtgc tttttgtccc acaacatacg cggaagacaa catggcaaac cagacaagac
     9901 cgaatcgaac aagctcttcc gtgaccgtat ttggatttcc taaaaccgtt ctggaaaata
     9961 cttgccataa aacaacagcg accatcccaa caagcaaaac agctcccagg atctctaata
    10021 ccttgtttaa aatcattttc atcacagcta cgctccttgc tcttggatct tttgataaag
    10081 gtctttgtag gtaggatcgt ttttgaatat atcatgaagt ggggcaactg cctctcgaaa
    10141 cggtgtatta tctacatcat tgaattggac tccatgttct tcttgtgcta aagctttttc
    10201 ttcttcaatt gcttttgcaa aagcttcaat ttcaaaaacc gttgattctt tcgctgcttc
    10261 ttcaatggct ttttcctgct catcagttag ctgttctttg atggcatcat tcatgatgat
    10321 gatatctggc acacgtgtat gctcatcata ggaatagtat ttagccacac caccatgtcc
    10381 agctgtatac aagacaaatt cattgttttc tgatccgttg atcaaattgg attgaagaga
    10441 cgtgtacact tcatcacttc ccataggtac gggcgaaccg cctaaaagct ctaccatttt
    10501 gatagcggtt tcgctttgca tcacacggat cttctttccc ttcaaatctt ccggcgtatg
    10561 gatcggaccg tctaccatat aaaagctgcg ctgaccagaa tcataatagg tcaatccggt
    10621 aaatcctaaa tttttggtcg attggtagat tggctccatt atttcttcat tatccatcac
    10681 cttaaagaaa tgttcttcat cattgaatag ataaggaact gaaaaaatcg aataatcttt
    10741 agagaaactt tccaacgctg aggcacttac ttttgtgaaa tctactgctc ctgactgtgt
    10801 caattcgatc agttcacgtt ctccacccag ttgtccatct gggaaatact ggatctgtac
    10861 ggaaccatct gttttttctt ttactaaacg cccaaattca ttcattgcat caattactgg
    10921 ctggctgttg cttgcataag caaagcgcaa ggtaactatc tcatttacat ctgcatctgc
    10981 gacagttgcc acactagaat tatttccgca gccgcttaga cctagcagac ctaggctaag
    11041 cgaacttgct gcaaacagca gtgatgtttt tttcatatgg gtttcctttc ttttaacaat
    11101 aagagacgag caattctgtc aaacaaagca ccgttaacga ctgtccataa ggcattgctg
    11161 tgattccgat attccgataa aaatctaaac tgtcccccat tcctgtcccg attgaaacgt
    11221 tttgaacttc tcctttttca tcgatttgtt caagcaatcc ttggatagca cgataagcga
    11281 cttctttata ttcttgtggt aaataacgtt tatggaccgc tttcaaaata ccgtaagcaa
    11341 atcctgcagt tgcagaagat tctacgtaag aagaaggatc atccaacaac gtatgccata
    11401 aaccgctttc agactgataa gtttttaatg cacgcacttg tgcttcaagt gtttcgatca
    11461 ataatttgcg caatgcatct tctttcgata attctaagat ttcaatgatc tctggaatag
    11521 caatcgtcaa ccagcagttt ccacgagccc aaaatgcttc ggcatagtta tgatttcctt
    11581 caaacgtcca gccgtgatac cacaatcctg ttcgtttatc catcaagtat ttgatatgga
    11641 tcataaactg gtaacgagct tcttctaaat attctgggcg attcaacagt ttcccgattt
    11701 ttgccaaagg aagcactgtc atcatcaatg tatcgtccca caactgattc ttattttccg
    11761 gtccatatgt ggcatgctgt aacccgtctt catttgtccg aggcatgtca ttcatgaccc
    11821 attctgccca ttgttctaaa taaggcaaat agcgtgaatc tttcgtgtct tcatacaagt
    11881 aagccatagt caatagcggt gccattgtat tgacgttttt gggcggagcg ccttcttcca
    11941 tacgatcctc aaaccattca ttgaccactt gataagcgcg aggatttttt gtcatctggt
    12001 aatttttata aataccatat agaccgacac cttgtggcca attccacact gtccagcttt
    12061 tgtcatctac tttcagtcca tcaaagtcca acaaaaattc accattatcg tctttgatcg
    12121 ttgtcaggtt ttcaacgagc aggtcaattt tttctttcac taattcttta ggtacaaaaa
    12181 tctcttcttt aataatcatc tttacgccac ctttttaaac gctttcattt tatatcctta
    12241 atatagcagt aataccgtcc actataaata tcgctttttg aacaaaaact gtctaaaatt
    12301 gacctaaatg ttaaataaca aaagtaactt tcagttactt ggaaattaaa aaaagaactt
    12361 ggagaaacaa cgttttccaa gttctttttc atctattttc ttatcttttt tctacgcttt
    12421 cttttgctta tccgttgtaa gcatcaatga tgatctgttt caattcagag atcaatggtt
    12481 ctttcggatt agctgtcgta cattggtctt cgtaagctaa ttcagccata cggtctacgg
    12541 ttgtatctag tgtttcttgt gtgactcctt gagcttttag actcatgttg atgccgacag
    12601 attttcctaa ttcataaaca gccgttgcta aagcttcgac taattcctct gttgtttcgc
    12661 ctttcagacc taagaatttt gcgatatctg cgtaatctgt atctgcacgg aaatagtcat
    12721 atttagggaa catcgcatgt ttttgtggat cttttgcatt atagcggata atatgcggta
    12781 aaagaatcgc atttgtccgt ccatgaggga tcccgtattc cccgccgatt ttatgtgcga
    12841 ctgagtgaca aatacctagg aatgcgttag caaacgccat accagccatt gtagaagcgt
    12901 tgtgcatttt ttcacgtgat tccatatcag gtgtttttac tgatttttct aaatagtcaa
    12961 agactaactt gattgcttgt aggctcaatc cacgtgtata atctgaagcc atgactgaaa
    13021 cataggattc gattgcatgt gttaacacat ccatcccggt atctgctgtg acagatgctg
    13081 gaactgacat gacgaattga ggatcgacga ttgcgacatc tggcgtcaat gcataatctg
    13141 ctaacggata tttcacatgt gtttcactgt cagtgatgac cgcaaatggc gttacttcag
    13201 aaccagtccc tgatgttgtc gggatacata cgaattgtgt tttttctggt ttgtcgatct
    13261 tatatgtccg tttacggata tccaagaatt tttgttttgc accaaagaaa gatgtgtctg
    13321 ggtgttcata gaacatccac attcccttag ctgcgtccat tgcagaaccg cctccaagag
    13381 cgattactgt atctggcttg aagtctgcca tcagtttcgt tcctgcgtat acggtatttg
    13441 tcgatgggtt cggttcaaca tctgagaaga cttcgatctt cacatcattt ttacgtcgtg
    13501 ccaatacttc acgaactgta tcgcagtaac cgaattggac cataccaggg tcacaaacga
    13561 tcatgacacg ttcaacgttt tccattttct ctaagtacaa tagagagttc ttttcaaaga
    13621 agatttttgg cggtaatttg aaccattgca tgttatttct ccgtttcgct acagttttga
    13681 cattgattaa gtttacagca gagacgttac gagaaacaga gttcttacca taagaaccgc
    13741 atcccaaagt caatgacgga atcatttcgt tatagatatc accgatgccg ccttctgcag
    13801 atggtgtatt cactaaaata cgacaagctt tcattcgaag accgaatttg acatgcaact
    13861 cttcgttttc tgaatggata actgctgtat gtcctaatcc tccaagattc aacatacctt
    13921 cacacaaatc aaacgcatgg tccgtgttgt tagctttgat catggcaaga actggagaaa
    13981 gtttttctct tgatagcgga taatctgggc ctacgccttc caattcagca atcagcattt
    14041 tcgttccttt tggtacactg attcctgcca attttgcgat ttccatcgca gaatgtccaa
    14101 cgattgatgg attgaccgca tattttcctt cgttcatcac tgcatcttct aatttttgca
    14161 attcatctgg ttttacgata taaacttggt gtgcttggaa ttctgctttt actgctgcat
    14221 agatttcttt atccacgatc acagcttgtt cagaagcaca aatcattcca ttatcaaatg
    14281 ttttcgaaac gatcaaatcg ttcactgcac gtttaatttt tgctgttttt tcaatataag
    14341 ctggtacgtt acctggacct acacctaaag ctggttttcc tgtagagtaa gcagacttga
    14401 ccattccagc tccacctgtc gctaagacga tcgcgatacc tggatggttc attagcatag
    14461 aagtcgcttc gatcgaagga tgttcgatcc attggataca attttcagga gctccggcag
    14521 cgatagcagc atcacgtacc actcgtgctg cttctgcaga agatttttgg gcacttggat
    14581 ggaaagcaaa aacgattgga tttcttgttt tcaacgcgat cattgatttg aaaatcgttg
    14641 tggaagtcgg attcgttgtt ggagttactc cgcaaaccac accgactggt tcagcaattt
    14701 caatcaaccc tttttggaca tcttcattga tcacaccaac tgttttatca tgtttgatat
    14761 tatgccaaat atattcggac gcatacatat ttttgattgc tttatcttcg taaacaccgc
    14821 ggcctgtttc ctctacagcc atcttcgcta atggcatatg ttgatccaaa gctgccattg
    14881 ccatttcatg cacgatatgg tctacttttt cttgatcaaa gctttccatt tctttcaatg
    14941 ctgcatttgc tttttctgcc aaatcatcaa tcattgcttg tacgtcaatt gtcttcgttt
    15001 tttctttttc tattacttta gccatcatta tcctcctaat gggtcatggt tatttgttaa
    15061 ataattcaca tgtatatcat acaatattta tttttctatg taaacacttt tttgtgattt
    15121 ttttcacagt tatttttaaa tataaaattg ctttatttac aggtgtttgt atagaaaaat
    15181 atataaagtt atttattatt atttatatga cacacccgat tattcaatat tgagcaatat
    15241 atcccatgct cttatctctt tcttttttga ataaaaaatg aggtaggaca tctttctgcc
    15301 ctacctcgtc tttaaataag ctttgatact tatttttctt ccttctcttc tgtctcttcc
    15361 agcgtttctg ttgtttcagt ttttacttca actgtctcag ctggttcttc tgttgtttca
    15421 acagtagtcg ttgcatcatt tgttacgaca gtccctggtt tgaccgtttt gattgctgca
    15481 cgatcgtact caaggaagat cccttcgcag tctaaaacaa ctgtccgttt gtcattgtcg
    15541 atttctgaaa caactccatg taatccgccg atcgttacga ctgaatcacc tggtttcatg
    15601 ctatctaaca agttttgacg ttcttgctgt tgtttctttt gcgaacgtga cataaaaaac
    15661 cacattgcta atagcgcgat aaacatcaca agcattggta atgttcccat ttttttcacc
    15721 tcactaattc tgtatctaca aaaaacactt tagcagaaaa atgctgataa cactagtttt
    15781 ttacttaatt tttaaaagat tttcctttag aagctttttg cgttttcttt attgaacccg
    15841 tattcttcaa aaaaggcttc tctaaattca agcaaattat catccatgat tgcttgacgt
    15901 acttgtttca ttagattcaa caagaagtat aaattgtggt aagaagtcaa acgaatccca
    15961 aatgtttcat cacatttgat caaatggcgg atataggcac gtgtatagtt tttacatgta
    16021 taacaatcac atttctcatc aattggacgg aaatcatggg catatttcgc atttttcact
    16081 actagacggc cttgagaagt catacatgtg ccatttcgag cgattcgagt aggcaagaca
    16141 cagtcaaaca tgtctactcc acggataaca ccatcgatca atgaatcagc agcaccgaca
    16201 cccatcaagt aacgtggttt gttttcgggg atcaacggtg tagtgaattc taatacacga
    16261 ttcatttctg atttaggttc cccgacagat aacccgccga ttgagtaacc agggaaatcc
    16321 atcccgacta gatctttcgc gctttgacga cgaagatctt caaaccctgc tccttggatg
    16381 attccaaata atccttgacg gtctggatta gcatgagctt tcaatcctcg ttctgcccat
    16441 cgagacgtac gttctactga ttttttcacg tagtcgtaac tttcatcgaa tggtggacat
    16501 tcgtcaaaac tcatcatgat atcagagccc aatttatttt ggatgttgat tgctttttct
    16561 ggtgataaaa acatacgcga accgttcaag tgatttttga aatgcacgcc ttcttcttcg
    16621 atattccgca tatcacttaa tgaaaaaact tgaaagccgc ctgaatctgt taaaatcggt
    16681 tgatcccagt tcataaattt atgcagtccg ccagcttctt ctactaagtc ttcaccaggg
    16741 cgtagccata aatgatatgt attacttaaa ataacgccag cacccatttc tttcaactct
    16801 tctggggaca ttgtttttac tgtagctaat gttcctactg gcatgaacat tggtgtgggg
    16861 aaagttccgt gtggcgtgat caattcccct aaacgcgcgc cagtatgttt ttctttcttg
    16921 attaaacgat agcgaatggc aggttgcgtc attcatttcc ctactttcta tataaaatat
    16981 cttgacaact cccaccatca taatcgtttc aagtgctttt tgcaagttcc ctcggcttcc
    17041 ttctacttta tttaatattc ttttttttat ccttttttta tgaataacaa aaaaaagaaa
    17101 aacattatga cagaaatatt ttttgataca tggtatattg gtgttattaa agaaaaggag
    17161 ggaaaaatca tgcaatcttc tgcagaatta aaacgccaag caaaagagag tctaaaagga
    17221 cgttggggac aagctgtttt actaaatcta atccctacat tgattggtat tgctactatt
    17281 cttcttatta tgattcctat tatcttgttt gccaccacca tatatgatgg atcagcgagc
    17341 gtctcagaag cgacaacttc aggaggcggt agttcaggag gcggtggcgg aattatttcc
    17401 agcatcatta gcgctttgtt catgagcggt atttcttgga cttatcttga tattttacgt
    17461 ggacaaaaag atcagatcga accattcaaa gatgcatttc gtggattcaa aggtttgttt
    17521 atcggcggag tgatcagctt agctgtgctg atctcgatct ttacaacatt atggggtttg
    17581 ctattagtta ttcctggaat cattaaaagc tattcttatt ctcaaagcta ttttatttac
    17641 tacgatatcg tccaagaaac aggacaaaca cctaaagtac tggatacgat cactgcaagt
    17701 cgaaaattga tggatggtta taaaggacaa cttttctggt tagatctttc atttatcggc
    17761 tggcatatct tagcattagc aactgctggt attggttatc tatggctcag cccatacatt
    17821 tctgcaacaa aagcagcatt ttatgaacaa ttgccaaaag atatttaaaa gagagtgtga
    17881 cttgtgaccg agggggtggc aggagaacac cgtttatcga gaggcagtaa aagaaacatt
    17941 cagcccatta gcaaaaagga ggtgtgacaa cattcatgtc acacctcctt tttgtatagt
    18001 tccagtaaaa agaaaatcaa cttagaaaca gttaatcatt cctcttcttc tgcttccggt
    18061 aatcgatgga acttgatcag ttcattcacc gcgattggta cgatagataa aatccctacc
    18121 aagaaccagt gattcaaact gatgtgtact aggccgaata gttcttgcgt aaatggcagc
    18181 aaagcaacag ccgttgtaat agctaatgcc agtaacgcca tttctaccaa agctttattg
    18241 gtcgctaaat tgactttgaa gatcgattgg ctgctgcgta cattgaacac atgcagaatc
    18301 gatgaataag ctaagatcaa gaatgcaaca gtttgaccga catgctgact tgcgtctacc
    18361 catgggctga tagtatcgac ataactacct aaataataac caaacaaggt tacgatggtg
    18421 aagacccctg catttatggc aattttttgc aataaacctc gagcaaagat cccttcattt
    18481 ttcggaatgg gtggctgatc catgatgccg tgttccgctg gctctcttcc taaagcaaat
    18541 cctggaagtc catcagcaac tacattgaca aacagtagtt gcacagctgt aaatggtgct
    18601 ccccatccta acagcatcgc aatcaaaaca ataaagatct ccgaaatatt acagcttagc
    18661 aaaaagttga tagctttacg aatattctga taaaccgaac gtccttgact gacagcgtct
    18721 acgatcgtcg caaagttgtc atccgtcaaa atcatatctg cggcactttg cgccacatct
    18781 gttcctgtga tccccatggc acacccaacg ttacttgctt tcaacgctgg tgcatcattc
    18841 acaccatccc cagtcatcgc tacgactgca cccgtacgtt gccaagattt gacgatccgg
    18901 atcttgtctt ctggcgttac ccgagcatag acagagagag ctttgacacg cgcatctaat
    18961 tcttcgtctg ataattgatg cagttctgat cctgaaagtg cttctgacgg atcattcaag
    19021 atacctaatt ctttcgctat ggcactggca gtcaccacat gatctccagt gatcatgact
    19081 gttttgatgc cagccttttt cgctcgcttg attgctcctt tactttctgg acgtggtgga
    19141 tcgatcattc cgatcagacc aatcaaacgt aagtcttttt ccagtgcttc agaagaaata
    19201 tcttgcggtt cttcgtcata agtagcataa ccaacagcaa tcactctcag tgcccgttta
    19261 ccaaagcgat cattaacgat cgccgcttgt tctacatcgc ctgaactaaa gcgcggagca
    19321 agaacatcga atgctccttt ggtgattgag atgtattttt cgcccatttg gtgaactgtt
    19381 gtcatcattt ttcgttcaga atcaaaagga agctcgttta ctcgtggata tttttcttct
    19441 aattctgcct ttgtatgata attctcttca acagccctta cgatggctac ttctgttgga
    19501 ttacctttga taacaaggtc gtcatcttct tgctcgaccg tcacatctgt acatagtgca
    19561 gccattttca atatttccat cgcgctgttc gtcattgaat cttcgatatt cgttacttca
    19621 tcttctttgc tccatacccg gcggacgcgc atttcatttt gtgtcagcgt tcctgttttg
    19681 tctgagcaga taacattagc ggttcctagc gtttcaactg ctggcaactg tcggatgatc
    19741 gcatgtttcc tcgccatctt ctgcacaccg tatgcaagtg tcaacgtaac aataacagtc
    19801 aaggtttccg gtacagctgc aacagccaag gaaatcgaag tcatgaacat ctcaaggagt
    19861 ggttctcctt gcagttcacc aatcacaaat actaaagccg cagccgctaa agcgatcata
    19921 ctgattcgtt tgcctagctg attcaatcgg acctgtaaag gtgttttttg catagtattt
    19981 tcgttcaaaa gatccgcgat cttacccatt tctgtagcca tgccaatggc agtgacgatc
    20041 gctttcccac gacctgcagc aaccgtacat cctttaaata ccatattaag ttggtcacct
    20101 aatgactcat cttcttcagc aataaatgct gcatcttttt caactgcttc actttctcca
    20161 gtcaacgccg attcttctac acgtaattgt gatgcttcga tcaaacgggc atcggcagaa
    20221 atagcatcac cggactctaa aacaagaata tcacccacta ccagttgctc tgactcaaca
    20281 gtggtcactt tgccgtctcg tatgacagtt gccgtttgtt tattcatatc ttgcaaggat
    20341 tccagtgctt tttctgcgtt tccttcttgg acaatcgcca ataccgagtt gatcacaacg
    20401 atagcgatga tcagcagtcc ttcaaataaa tccccatgtt ctgtagcaaa tgcggtataa
    20461 aaggaaatca ccgctgctac aagaaggatg atcgttgtaa aatcggacaa gcttcgaaag
    20521 aattttttga tcatcgattc tttcggcgcc tcatcgaatt tattcaatcc ctgctttttt
    20581 cgccgttcat ccacttcttg tgaagacaac ccagtatctg gatctgcttc tgttaccttc
    20641 tttacttctt ctattgtctg cttgtattct tccatcctat tcctccttta aaatcttcgt
    20701 acaaaaaaag gtacttattt tactataaca tccttttttt atttacttca aataaagggc
    20761 ttatcgatta tttgttaaga aagcgtaaaa tttaaagtgc ttatatttgc aaacgctacc
    20821 aaaggatgct attatataga tgtaaggaag gaggatacca tcaaatagac agaagaattt
    20881 ctcgatagaa cagtgtaagc aaacaaagtt gttagaagta caggtttcac ccagacatgc
    20941 gtgtagagta caaacctata aatagtgaat gcgtatggat gtttggatca cgaagtatcc
    21001 gaaacaagaa gtaatgcgat acgtaacaat tgaataacgt aaacgtctga aaattgaata
    21061 ggaacaagag atgtttcaaa tgttgaagat gtaacgaaaa caaaattttt gtttaacatg
    21121 aagaaattca acaccgtgct ttgcatgtat gatggaaagt tacaatcatt tctgtttatt
    21181 tgaatccttc cgaggtacaa gaaagccgag cttatcgaga gctcggcttt ttctttgtca
    21241 taaaatcttt gtaaagcttt tttgttattc aaaactagcg gcttttcata accttatttc
    21301 acgaacatcg catctccaaa actaaagaaa cgatagcgct cttcaatcgc atgctggtaa
    21361 gcagataaag tcaattctct tcctgcaaag gcactaacta acatgactaa agtcgatttt
    21421 ggcaaatgga aattagttga gaaagcttct accactttga attcatagcc aggcgtaatg
    21481 aaaatatcag tccatccgct gtctgctttg atttctccat taaatttcgt tccaatcgtt
    21541 tctaatgttc gaattgaagt tgttccgaca gccacgattt tcccgccatt tttacgtact
    21601 tggttcaatc gctcagccgc ttcttccgtc aaacggtaga attcactatg catatggtgt
    21661 tcttcaattt tatctacgct gacaggtcgg aatgtaccta accctacatg tagtgtcaga
    21721 tagactaatt caacaccctt tgcttcgatc tcatcaagta attctttcgt aaaatgcaat
    21781 ccagctgttg gcgctgcagc agaaccattt tctttcgcat agacagtttg atagcgatct
    21841 gggtcttcca gacgttcttt gatatatgga ggcaatggca tttctcccaa tgattcaaga
    21901 atctctagaa aaattccttc atactcaaag tcaatgatcc gtccgccgtg ttccaattct
    21961 tctttcacta ctgcttgcaa tctgccatca ccaaaagaga ttcttgtccc aactttggca
    22021 cgtttcgcag gtttgatcaa tgtctcccac gtatctcctt gtgtattcgt tagaagaagg
    22081 acttctaaat gcgcaccagt atctggtttt tctccatata aacgagcagg cagaactcgt
    22141 gtattattca taactagcgc atcaccaggc tgtaactctt caatgatttc atggaaatgt
    22201 ttatcttcca tctctccagt tttccgatcc aaaacaagta aacgagaatg atcgcggtct
    22261 tttaatggtg tttgtgcgat taattcttcc ggcaagtcaa aatcaaaatc ttctgttgtt
    22321 aacatatcgt cactcacttt ctatcatgtg cttcatcatt ctatcatgtt tttcgttctg
    22381 ttggcaaaat cagcactttt tcacttctat tttttgtttt cctaaataaa aaaactaccc
    22441 tatttgtatt ttttcattgt tttaggcata ctagtaatag atagttcttc attgaaaata
    22501 gaaaaagagg tggcaccatg attgaatttc aaaatgtgtc aaaaatttat aaaggtggaa
    22561 aagtcgctgt agaggacgtc aatctttcat ttgaaaaggg tgagttcatt tgttttatcg
    22621 gtacgagcgg aagcggaaaa acgacgacga tgcggatgat caaccgaatg actgatccaa
    22681 cacaagggaa aattttgatt gatggaaaat ccatccaaga aattgatcca gtggaattgc
    22741 gacgtcagat cggctatgtt atccaaagta ttggattgat gcctcacatg acgatccgtg
    22801 aaaatatcac tcttgtccaa aaattgttga aagtcagtca agaagaacgg aataaaacag
    22861 ctgaaaaaat gattgatctt gtggaattgc cccgtgagat gttagatcgc tacccacatg
    22921 aattatctgg tggtcagcag cagcgtattg gagtcgttcg tgcccttgca gcgaatcaag
    22981 atatcatctt gatggacgag ccattcggtg cgcttgatcc aatcactcgt gattccttgc
    23041 aagaccttgt caaagattta caagagcgtt tgggaaaaac gatcgtattt gtaacccatg
    23101 acatggatga agcgattaaa ctagcaagcc gtatcgccat catgagtgaa ggccgtgtga
    23161 tccaatttga tacaccagaa aatattttac gtcatcctgc aaacgatttc gtagaagagt
    23221 tgattggaga agatcgccta ctacaagcca agccaaatgc gacaagagtc ggcgaagtta
    23281 tgctaaatac agcaatcacg attacaccgg aaaaatcatt acaagaagca atcaaactga
    23341 tgagggaaaa acgtgtagat acgttgttag ttaccgataa ttcgaatgta ttaaaaggat
    23401 ttatcgatgt agaaacgttg aataaaaaac gaggaaaagt atccagtgtc ggcgacattt
    23461 tgaataaaca tgttttttac gtaaaagaat ccgcctatct tcgtgatacc ttgcaacgca
    23521 ttttaaaacg cgggctgaaa tacgtgccgg tcgttgatga acagaacaaa gtcgtaggta
    23581 tcctgactcg tgcatcatta gtggatatcg tctacgatgt gatttgggga gaagatcccg
    23641 caactactga tgttgcagag tcagctactc ctgaagaatc taaggaggtc tgacctatgc
    23701 agcagttttt tcatcagcac ggttcagaaa ttctctcaaa aggttttgaa catctttata
    23761 tttcaggact tgctttgatc attggtatca tcattgctgt accgcttggt gtcttactga
    23821 cacgttttcc aaaagctgcc acgattgtga ttggcttaac cagtgccttg cagacaatcc
    23881 cttctcttgc tttattggca ttgatgatcc cgctatttgg cgtaggtaaa ttacctgcga
    23941 ttatcgcact ctttatttat tcattattgc cgattttacg taatacgtac attgggatga
    24001 aaaatgtcga ttggaattat cgggatgtgg caaaaggcat ggggatgact gagttccaat
    24061 ccattttttc cgttgaattg cccattgcta tgccaacgat catggctggt attcgtttag
    24121 ctgccgttta cgtgattgca tgggctacac ttgcctctta tatcggcgct ggtggtttag
    24181 gcgatttgat ttttagtgga ctgaataatt accaaccaga tctcattttg gcaggtacga
    24241 tccctgtaac aattttagca ctattggctg attggcttct aggtctcttg gaacaccggc
    24301 tgacaccgat cgcacttaga gaggagaatg aagaatgaaa cgtatactaa aaatgaccat
    24361 ggttcttttc tctcttctta tacttgcgag ttgttccttc cctggtcttg ccagcaatac
    24421 agatgaagac accatttcga tcacaggtgg aatcacttca gaagcacaga tcttagcaag
    24481 tctagttgcc ggaatggttg aacattatac agataaaaat accgcaatca tcaacaatct
    24541 ggctactaca actatcaacc atcaagcgat gatgaacgga gatgcttcaa tttcagcggc
    24601 tcgctacaca ggaacagatt taacgactac attgaacctt cctcctgaaa aagatccgaa
    24661 aaaagcgttt gctaccgtga aagatgaatt tgagaaaaga tacggtcaaa cgtggttccc
    24721 gtcctatagt tttgaaaata cgtatgtctt tcttgttcga aaagacaccg cacaaaaata
    24781 ccatctatct aaagtcagcg atctgaaaaa tgtagcggat gaattagtag ctggtgtaga
    24841 tacttcttgg atcaatcgta aaggagacgg ttatgacggc ttccaagaaa cttatggctt
    24901 ctccttcaat tcgatattac ctatgcagat tggacttgtt tatgacgcag tcgaagcagg
    24961 aaaaatggat attgttcttg gctactctac ggatggacgg atcgccagtt atgatctagt
    25021 catgttggaa gatgatcgtc aattcttccc accatatgat gctgctccag ttgtagacaa
    25081 caagatatta gctgatacgc ctcatttaaa agaagcctta aataagttag gtcataccat
    25141 cagtacggaa aaaatgcagg aattgaacta cgaagcagat aataatctag tggaaccatc
    25201 agtagtagca caacgtttct tacaagaaaa ccactatttt gaagggaagt gaagcaattg
    25261 gataaaatga atgtctttca acaatttttc tattatttcc aggaaaatgg ctcctatatc
    25321 tttgcacaat ttatccgtca cttcctgatt tctatctatg gtgtcctctt tgcagcagtt
    25381 gtcgggattc cggttgggat catgatttct aggagacgta aattagcaaa ctgggtgatt
    25441 cgtttggcga atatcatcca gacgatccct tctctagcca tgatctccat tttaatcatt
    25501 ggattaggcc tgggagtcaa tgtcgtgatt gttactgtat ttttgtattc cctcttaccg
    25561 atcatcaaga atacgtatac cgggatgatc caagtagaca aaaacatcct tgatgtcggc
    25621 aaaggcatgg ggatgactgc tagacaacga ctattcatgg tagagttacc actctctgtt
    25681 tctgttatca tggcaggcat aagaaacgcg ttagttgttg ccatcgggat aaccgcgatc
    25741 ggtgcattcg tcggtgctgg tggtcttgga gatatcatta tccgaggaac caatgcaaca
    25801 gagggcacat cgattatttt agcaggtgca ttgccaactg cattaatggc gatcatcacg
    25861 gactggcttt taggtatttt ggaaagacgg ctagatccag ccagccgtac atcacgctga
    25921 tgttattgta aaaaacaaaa gcttctgcga ccatttgttg gtcgcaaagg cttttttagt
    25981 tctgtcattt ttaaggcaat aatccactct gattcttccc tccttatggg caaagagttg
    26041 cttttcttag ctatttatcc attccgtata ctccaacttc atactgccaa cgggttcata
    26101 tctgcatcca ccgactaagt ttttttctta tccgctaagg cattttgcca atccattttc
    26161 ccatcactcg tcatggaatt cataccacat gatctctaaa tactcgtctg cttgttcttt
    26221 gaattgggca tcaattctta catcctttgg cgtggctttc atttaagcct tttccatcag
    26281 tcctcgattg ttcgatgcct tcagctgcat catcaaaact ggtgactgcc gtatcggtaa
    26341 cttcgatcat cttttataca atcgggaact tgccatcctc atccttggct aaaaacatat
    26401 ctagccattc cagcttctcg ccatgggagg tttgcggttc ctaaaaccgg tacacgattc
    26461 ttatggactg catccgtcgc aactagactt ggcgatacga tgatccttca ccagaagagc
    26521 acccccgtaa accaattgat cgatatattg ccaataagaa aagacattgg cgtaaaagat
    26581 atcgatccct cgtgatgcat tgccgttgat actgctattc atggccgaaa gcgccacgac
    26641 tttaacttct tccgtttgag tgacattcat gttcggcaat gtcgctttgg atacccgctc
    26701 tgctaaagga acgacactgg tattgtattt cgtatctgta tcttttccaa aagaccatgc
    26761 caacgaatcc tctgggaacc aataagacga ctccggttga ttgtccattg cttttttcaa
    26821 catacttttg gctgtttctc gataaacgac atttccaatt gtcttcgcct cactttggcg
    26881 ataatcgatg gtcgttgcca ctgccaacac taacaacgat gctccaattt tactgatttt
    26941 ccttgtcaca cttcttcctc cttgttttac ataaacccgt caacattcca ttcctaaact
    27001 caaacaatga cccttcaaag gtggagatat aggaacaaat taaagaaatc atgcaataaa
    27061 tccaagaagg ttggttttag aacaaaaaaa gagtctcctt tttggggaga ctctttgtta
    27121 actatctaaa aaggttggct cttctcactt cactcataat gtgtccaggc agaataaatt
    27181 tctacaactt tattttcctc atcaactttg taaaccactc gatgctgtct attcagcctg
    27241 cgtgagtatc tgccttcatg tgtaggtctc agcttttcaa aagactgcgt gggcatatat
    27301 ggatcttcct tcaatgtctg aatcacttct tcaaattgtt tctttaaatt cgtttgcttg
    27361 atttttctta aatccacttt cgcagagttt ttgattgcga ctgtatagtt actcatcaaa
    27421 gagcttccca atcaatatca tcgatattcg taaagctact gtcgtctttt tcgcggtctc
    27481 gtactttatc taaagtacct gtttgctcaa gaaaaagggt ttcttgaatc gctctccagt
    27541 cttccagccc aatcaataca gcattgtttc cactacgatc actaataatc tcgatttcag
    27601 tatgattttc atttacctct ttcaataact gataaaaatt tttacgagca gtgctaggat
    27661 tcatcgttgt attagccatt gtctctcctc ctttttctga tataattata gcgtacttga
    27721 ttaagtacgt caaaaaaata caataaagca cttaaattca gcaaactttt cctctttttt
    27781 acatagagaa aaagacccct ttttagggag gatctttgtc cacgctcact tcttactcct
    27841 ttacgactag cggtagccaa acgtaaagta attggtaaca acgtatcatc tttagcaaag
    27901 gtcgaatcca aagcctgtga gtctatgcaa caacaccaat acacgaaggt tcctcgttgc
    27961 agtgtgactt gattgatatc ttgaataacc tgaggatacg cacgaaagat ttgtaacggt
    28021 tattcaaaac tgattttggc cgtcaagtct tctactgcct caaaaatgat tgcctctttc
    28081 gtctcctgtc attcatgttc actatcgatt tgatacttgc ttagaccgtt gggtttgtga
    28141 atcaccaatt aaaaatggca ctgagttatg gtaacaaaaa atggtcactt gacgaggctg
    28201 agattcctac ttatgaagac acatggcact taagagtcga ggtaaaggaa gaagctgaaa
    28261 atataggagg ctgtacatta tttggcaaag gattcggaaa ttatgatcaa gacatcaaaa
    28321 tcaaaatgtt ttacagctaa ataggaagaa taataacatt tcatacgctg ttcccatctt
    28381 taatggctta tagagggcac ctttagctga gaataagaca aaacagaccc tctagcttca
    28441 attaagagct agagggtctg tttttgattt taaaacttgg tttatttctt caaatggtag
    28501 ctataggtgc tgacaacgat attttcacgc caagacaatc gcaatcttaa ccgcaaactt
    28561 gtttggttat ggagaatatt ctgccagcgt ctgtttggta caaattgagg aatcaacaca
    28621 gtagttgtat agttttgttt cgctgcattt ttacttacga tatctacata acggataatt
    28681 ggattttcaa tcgagcgata agaagagtgg acgattgaaa aacggacatc tgggaaatgc
    28741 tttttaaatt ctgcttcgat ttctttttct ttttcgacat cttcgtctag cgaaacatgc
    28801 atcgcaacga cataatcacc gatcgaacgt gcgtaattca acgcaccgat atttacccgt
    28861 gtcacattcc cgactaatac aagaacggta ttcccgtcat aatcatgtag ttttgcatca
    28921 ttttctaaac gaagctgttc tgctactttt ttataatgat catggatctt ataaaaaatg
    28981 aacataacga ttggcataat gatgaagaaa ggccaaatgt cccctaaccg atagacaaac
    29041 aaaatcgcaa tgattgcata tgagatgaac gcacccgtaa tattggcaat cgatttgctt
    29101 aaccatcttt ttccttcttt tttccatttg acgaccatcc ctgtttgaga taaagcaaat
    29161 ggaatgaaca cgccgataga gtataacgga atcaagcgtt ctgttgagcc atggaaaata
    29221 aacagcaata cgatcgaacc tagcgctagt gtgataatcc cattggaata gcctagtcga
    29281 tccccacgat cttgatacat gtgaggcatg aatttatctt ttgccagatt gtatgcaaga
    29341 actggaaaag cagaaaaacc ggtgttagca gcaactgcta aaatcagcgc tgtagcaaat
    29401 tgaaggacgt aatacaaaat accatgtcca aagacagctt taccaatttg agacaaaaca
    29461 gtgacttctt tttcaggaac gatcccatac caatagttga taaatgtgat ccccacgaag
    29521 aagaaaccta agatcaatgc catcatcgtc aatgtagcag cggcattttt tgctcttggc
    29581 ttcttaaaga atggtacagc attactaatc gcctccaccc cagttaaaga ggaagaacca
    29641 gaagaaaatg ctcgtaaaat caaagcgata gaaacacccg gcacagccgc tcctggaagt
    29701 gcagtagctt gcaatggcac agcacctgtc acgatattat ataaaccaac aacgatcagt
    29761 aacgagatca caaggataaa tgtgtacacc ggaaataata ggaacgatgc agattccctc
    29821 agcccacgaa gattcagcat catcagtagc aaaacgattg tcacagaaat agcgacttga
    29881 tgaccgtaga gtgctggcac cgctgatgtg atcgcttcag cacctgctga gacagatact
    29941 gctactgtca acatgtagtc gatcaataaa gagccaccgg aaatcaagcc agcattcttt
    30001 ccaagattct cactgctgac gacatatgct cccccgccat gaggataagc atggatgatt
    30061 tgacgatatg atagcgttaa tgaaatcaat aaaataataa caaaagcggc aatgggtagc
    30121 gaataccaga tggctgcagc agacaaagcg actaaaacga caacgatttg ttccgtacca
    30181 tatgcaatag atgataacgc atcagacgac aataatgcca aagcagcaaa tcgggttaat
    30241 ttgtgctcat cattttcagc agattttaaa ggcttaccta ctaacaacct ttttaagtaa
    30301 tccaatttct aaactccttt acacacaatt ttacttcaag ttttatagtc gaaaatttat
    30361 agtaaacaag atgttatagt acgctcactg accttaaaag tcaataaact ttaagaaaat
    30421 aattggaaaa aaacagaaaa atatcaagcg ctttttcaaa cgctttaata cagcaaaaat
    30481 ctaacatttt tttatgggct ttttctttga attctataat aaaaggatag aacttgactt
    30541 agaaaatcaa gttctatcct ttttgatatc ttaacttttg gaagagagat gtaaggctgc
    30601 tttctaccca tcattcttac tactcgaagc atagcgcagt tgtcacaacc tcttgataaa
    30661 cggtgtttaa aagcttgcat ctgcggtaat aagcccaacc aaattaaaaa cataagaagc
    30721 tgtttttatt tggtcgttct tattacttga tgcagagcag cttttttaca acctcttggt
    30781 acacggtgtt caatcagctg accctcggta ttagctcaaa aaaacggcaa aatatgagga
    30841 actatttttc catttttttc tctaatactc aggtcaaaac actgatttca caacctcttt
    30901 ttttaatgaa aaatatggct ctcatgcgcc gcttctttga atttcgtcat tttttcttcg
    30961 atgaacggat agattgccgt tcctacgatt ccaaagacaa caaacaaacc gatcatgatc
    31021 cagattgctt tcgtagcatt tggccaataa attcctcctg cagattcccg taacaagttt
    31081 accgcatgag taaatggcaa gaacggattg atcatttgga agaatttccc tgagacttgg
    31141 atcggatagt tcccgcctcc gccagagatc gacagtacta agatgataat agcgatccct
    31201 ttaccgacat tatcgaataa tgcagccaag acatagacga tcatcatgaa cgctaaggcg
    31261 atcaagacag caaatgacac gctgtagacc ggattttgga catctactcc tagtaagaaa
    31321 atattcccta atgtaacgat caagctttgc gcaatcgcca ttgtcaaaaa tgtcagcata
    31381 cgagcaacga attgttctcg tttcgtaaaa ttgtttcggt ctttctttcc taaatagtat
    31441 tctgtcgtag caacacttga aagaagaaca gcgccgaccc acagacatag tgcagtataa
    31501 aacggggtgc tagctgaacc gttatttgcg attggataaa gcgcattggt ttgtagctca
    31561 acaggttgtg taaagaagtc actttctttt gttgcgtcta gtttcaataa tttgatgact
    31621 tcgcctagat cgacttcttg ttcgcctttt cggatcgcgt ctgctgcttt ttggatacca
    31681 gctttgatat ttggccagtc attgttgatc aagtccgcag cagcgttcac agctgtttcg
    31741 acttctggca ttttttcatt tactgttgtc aatgtaccgg tgatttcatt ttttagtgct
    31801 ggccaatcat tttggataaa atcagcggcc aatcctaatt tttgctgaat gatcggtaat
    31861 tcattgttgt ataggtctgc cccttcattg atgccattga cgatggtatc catatttcca
    31921 ttcagcattg tattggcatc atggatttct tgtccaattg caggcatttc tttttgatat
    31981 ttttctaaaa ttgccactgc attcgttaca gttgctttcg ttgagctgag aagcgaatca
    32041 aagtcgatct ggtccgcttt gctcagtgcg ccttgtgcag tcccaatcgt atcgatgact
    32101 tggtctagta gagaagtgac agtggaactg atttgacctg tattgatacc agcaatgatg
    32161 ccattgatat tattaacaac ggcatcaatt tgatctagca attcatcaat agccttcgtg
    32221 tcttcatttt gaacagctgt gttcaaagca gataggcggt tttgcagatt gcttagcaag
    32281 ggttttaatt gatttagctg atcaataatt ggctgtaatt tcgtatttcc agcacttgtt
    32341 tgcaatttag tcaggaactc gaccagttta tcgattgcat tttgttgttg agatattccg
    32401 tcaataaagc cgttcaatac attgctgatg tttttctttt catcatcagt cagcttacca
    32461 tcttcaatag ctgttcttac agtatctatt aacgaacctg tcgttgttgc cacttgtcca
    32521 attgacttca atgtaacatc tacactgttt gtgatgcttg gtatcgcatc tttcagctga
    32581 gaggcacctg attttgtttg gtttgctaga tcacttgcct gattttctaa atttttcaca
    32641 tctggcaaga tatcttgtac ttgttggatg atcgtcaagc cttgttttgc ttcatcgatt
    32701 ccttggttca ttgtctcttg gatcgaagca aaatcgccgt cgatctctgc tagttgctta
    32761 ccggcatttt ggatctctgg gattttttct tgcaaatcaa ggatgatttt tgcttgatct
    32821 ttcaactcag gcattttgtc attcaatgca acgacttttt cacccatctc atctacttta
    32881 gggatgtaat ccacgaattc atttgcttta ttcagttttt ccttgatttc aggtagttga
    32941 ctttgtaatt cgaggacttg ttttgtgtag ccgtcgatcg tgtccaaatt ttcatctgtt
    33001 gataaaatca tatctttaac tttattgata cttactaggt tcgtatcgat gtcgtaacct
    33061 atctcattga atactttcaa taaagtcgaa ctggctgttt tgataaattc atttgtgatt
    33121 tgcgattgga tcgaacttgc tcctttatcg gtgattttcg gtgcaatcgc attgattttt
    33181 tcatttaccg tatattcgat ttttggtttc ttgatatcac cactagtaaa actcagcaga
    33241 tcttctgaga aatcctttgg caagtaaata cctgcgtaat actttccgga acggactccg
    33301 tcttctaatt gttcttttga atcaacgaat tgccatccta attgtttatt tttatgaagc
    33361 gattcgatga cttgctcccc aatggccact tctttacctt gaaactctgc tggcttgtct
    33421 gcactgtaaa cagcaattgg cagctctcca gtatttccat atggatccca taatgctttg
    33481 atgttaaacc acgcatatag cgaagggatg atcatgatcg cgatgatcag aagtgttgca
    33541 atcgggtttt tgaaaatccg tttccagtca agcaaatata atcgaagcgt atttttgatt
    33601 gactgcatag ttctccccct tcttcttttt tcagctgaat tactttacca tgtatttcat
    33661 acttttttca atcggctttt ttcaaaaaag tcgaattttt ggacatatca cggcaattgt
    33721 ctcattgtaa tctcttacat attgagaaat aattaaggat tacagccttt actttatcat
    33781 gaaataaaag ggaaagataa ttttatgcca aagtttttat ctattatacg tatacgcaaa
    33841 aaagctaaaa cattttttgt ctttgcccct acaaaaatgt ttcagctttt ttcgtttttt
    33901 attcctgtat ttcttatttt tcgaatgaca ggatgatttt cccagcagga ggatgtcctt
    33961 caactagctg atgtgcttgg atgacatgct taagtgaagc agacagattc attgaaataa
    34021 aaacatgaaa ggctcctttt tggtaggctt tgattatgcc agcaaaggct tctgcatcta
    34081 gatattcctt tcttggaaca aacgattcat aaaagccttc ctttttttgc cgcaaatcta
    34141 gatctgggag gtcatttaat gcaacatacc tgccgcctgg tttcatgatc tggatgccgg
    34201 tttctccttt gatacttcct ttcgtcgcat cgattacaag gtctgcctga tcagcaaatt
    34261 gcagacctgg atttgtttta tcataagcag caaatgctga agcttctaat ttttttacct
    34321 tttcctcatt tttactggat gcactggtta agattcggat tcccttttca tggagaagct
    34381 ggatcagact tgacccgacc gcacctgatg ctcctagtat cattacagta tctgtcggct
    34441 ggatttccgt caaatggttg attagattat aagccgtaat tcctggcgtg accattccgg
    34501 ctgcttcttc ccaactcatt ttatctggaa tttttgctac ttttgctgat ggcaacacga
    34561 cttcttctcc gtaagtcccg ccgactgcat ggacagctac tcgatcacct acatgaacag
    34621 ttgtgacatc tgaaccaatc tctgtcacaa taccagcgcc atcattacct ggtacataag
    34681 ggaatttcaa tgttcgtatc tctttcattt ctcctaaacg caaggcaaca tcataaggat
    34741 tcacactgaa tgcttttatt tctacacgaa catgtccaga accgacttct ctgggatgag
    34801 catcgatttc ttcaaaaaca tcttgtgctt ctccgtaacc gttaattgca aatcttcgca
    34861 tggtcaccat ctcccttttt ctttaagttt acgtttatgc ctttattttg acaaaaaaaa
    34921 tgtcttttcg ttttggcgca tgattcaaaa caaaatagtt gtcttttttc gcgtaacgtg
    34981 ttaaattatt tatctaaaag cgtaaagatt acgatttacg ctaataaaaa ataattggag
    35041 gaatttgttg atggctaaaa aatcaaagat tgccaaagca aaaaaacagc aagaaacgat
    35101 tgaacgctat gctgctttac gccaggaatt aaaagcgcaa aaagattacg aaggcttgcg
    35161 taaacttcct ttagatgctc gtccagaacg attgaaaaat cgtgatttaa tcgatggacg
    35221 ccctcgcgga tacatgcgta aattcgggat gtcccgtata aaattccgtg aattagcaca
    35281 taaaggtttg attccaggag taaaaaaagc cagctggtaa tccaagtctg gctgtaatgt
    35341 aaaggaggag ggcgcatgga ccactccttg gatcatcaaa aaagatacgt tcgcttcttc
    35401 tagcagaaat gctggtacta gaagcagacg tatctttttt tacgcacagc tggtcctttt
    35461 ttatatgtaa aggctgcatg gttacttcgc tatatttatt tagtaacagg agtcagtgct
    35521 tcgataccca cactaatttt caatgctcgg tcaacttctg ccataatggg tcgatccaaa
    35581 tggcagacct tttctttcaa tcttgattta tcgatcgttc tgatctgctc caacaggata
    35641 acggagtctc tttctattcc tgttccagta gctttgatcc caatgtgagt cggaagctta
    35701 ggtttagcca tttttgccgt tatggccgca acaatgatcg tcgggctaaa atggttacct
    35761 agattatttt gaataaccaa aactgggcgt gttccgcctt gctccgatcc cacgactgga
    35821 gaaagatcag caaaataaat atctcctctt tttaccatag gatcctcctt attcttgata
    35881 ttctcttggt atccgctgtc ctaatccgca agccacttca tagtggatcg tacctaattg
    35941 atctgctata tcctgcatag tgatttcttt gtctgcattt ttgccgataa gcgtcacttt
    36001 tgttccgaca ggaaattctt gaggcaatcg gatcatcagt tgatccatac atacacgccc
    36061 aatcgtttca cagtagttcc cttctaccaa tagagaaaaa ccctgcatct ttcgtggcca
    36121 gccatctgca taaccgatcg gtatcgttcc gatccactct gcttcaggag tgatatatgt
    36181 ttctccatag ccgattcctt cacctgctgg gagttttttg acttgaatca attcagaaac
    36241 cagctcaagt gctggctgca atggataaac ttccggtagt gcgtgcccag agggattaag
    36301 tccgtacatt gctactccat aacgaatcat attgccgatt gtttcatcat gccataaagc
    36361 cgttgcacta ttgctgacat gtacatatcg aggaagttcg ggaagttttt tcagcacttc
    36421 tataaagcgt tctttttgca gattccaata tgtatcatct gcctgatctg ctgtagaaaa
    36481 atgggtaaat aaaccttccc attctaatac acgactttct ttgatgaatg cagctgcctc
    36541 ttttacttct tcaggtgtac aaaatccgat acgacccatc ccagtgtctg cttttagatg
    36601 aatcgaaaga ggcgtctcta gcttactttt ctctaaaaca gcagctgctg cttgcaacca
    36661 ttcccgtgta cctgcagtta ctgataaatc atgagcaaca gctacagtca gtgcgtcgta
    36721 aggaaccata ttcaagataa ggatgggctg agtaaatcca gcttctctca actcgactcc
    36781 ttcatctaaa tttgaaacac aaaagcccga agctccacct gcaactgctg cctcagctgt
    36841 ttcaatagct ccatgaccgt atccgttcgc ttttactact gcaaacaatt cttttccttg
    36901 cggaagtctt gcaacttcat ttgctacatt ttcagttata gcttttttat gtatcactgc
    36961 tttcgttggg cggtgccaag cgacaaccat cgtttttcct tctttctagt cttccaaaat
    37021 aatttgtgca aaagctgttt catttgtatg agtaatactg acccatacat tgccttcatg
    37081 aggtgaacgc gtcacaacag gctgaccatt ttcatttttt aaaatctcga catcttgaaa
    37141 ggtaactttt ccgatcccag ttccccaagc tttagagaaa gcctctttgc aagcgtaacg
    37201 cccgcctaaa aattctactt ggcgatgaaa aggcaaggac tggaatagtt tcatttcatc
    37261 agaggtcaag attcgtgcaa tgaattttgg tttttcttcg atcagccgag tgatcctagg
    37321 aagttcgact gcatcgatcc caattccttt tatcatatgc ttttctcttt tcaataaatt
    37381 tagaacactc tcattctacc aaattataac cattttgcca caaataagtc cacaaataac
    37441 aaaaatgaaa gtttctctta aaaaagagaa actttcattt tatgtttaat cgttatttga
    37501 acggattaca aatccgcgtt ttttatcatt gtgacgttta ccgccatctt tttggctgtt
    37561 attatttttg ctataaccgc cttttgattt gtttccacga taattaccgc cgtcacgacg
    37621 attacggttg tttccgccac cacgtttgcc atttttattg tatccttttt tctgcattgg
    37681 caatgggcgt tctggtgtga tcttcactgg cactgcatca gctggatctt ttgctgtcgt
    37741 ttttagcaaa agtgcaacta aatcttgtgc agaatattct tctaataatt tatctgctgt
    37801 ttgcaggtat ttatctagac cattttctgc aagttttgtt tcgatttgtt caactgcagc
    37861 acctaattga cctttgaatg cttctttttc ggttggtgga cgtaaagtcg tcatccgttt
    37921 tttcgtcaag ttttcaatca catgcaagta gtccatttca tttggtgtaa cgaaagtcac
    37981 agacataccg ccttttccgg cacggcctgt acgtccgata cggtgaacgt agctttctgg
    38041 atcttgaggg atatcatagt tgtacacatg agtgacacct gaaatatcta atccacgtgc
    38101 agcaacgtct gtcgctacaa ggatatctaa atgaccgctc ttaaatgaac gcaatacgct
    38161 cattcgtttt tgttgagaca aatcgccatg gattccttct gcacggtaac cgcgtgcttc
    38221 taatccgcgt gccaattcgt ctacacggcg ttttgtacgt ccgaagacga ttgttaattc
    38281 aggtgtttga acatcaaaca agcgagtcat gatatcgaat ttttcgtatt cttttgcgcg
    38341 gacatagtat tgatcgatca agtcagctgt catttctttc gctttgatct tcacatgatg
    38401 tggatttttc atgaatttaa cgccaatatt cttgatagct ggcggcatcg ttgctgagaa
    38461 aagtaatgtt tgtctttgat caggaacttg tgaaatgatt ttttcgatat cttccaagaa
    38521 gcccatattc aacatttcat ctgcttcatc caaaactaat gtttgaacgg ttccaagttt
    38581 caatgtgtgg cggttaatgt ggtcaagcat acgtcctggt gtacccacaa cgatatgtgg
    38641 acgatctttc aagccacgga tctgacggcc aatatctgct ccgccgtaaa cggcttgtac
    38701 acgtactttt ttgtcgcgcc ctaaacgata taattcttct tgtgtttgga tagctaattc
    38761 acgtgttggt gcgataacta aaccttgtaa ttcgtgacga tctggatcga ttttttctaa
    38821 cattggtaat ccaaatgcag ctgttttccc tgtacctgtc tgtgcttgtc caataacgtc
    38881 ttttccagct aatgctagtg gaatcgtttc tgcttggata ggcgttgctt cttcaaaacc
    38941 agcgcgttcg actgatttca acaattctgg tgataattct agttctttaa atttcaaatg
    39001 aatcctccta tatggttgtt aactgaggat gtagcttttt tgctaccccg tcgatttgtt
    39061 cggtcaaagt gtattgtttc tttttgacag tggccacgca cgtttttatc cattcttagc
    39121 ccctgtggca ccaccgaaaa atcactttgc gaagtttagg cattagaaaa gccaaacggg
    39181 tcgcgttcag ctttatcatt attatgacat ctggcttcaa caagttgttt ctatggcaaa
    39241 accagtccaa tttctcttca tctttcggca ataggttatt ctaacatagt ttttcgtttt
    39301 ctgcaagttt acagattttt atttcatttg ctgaagttgt tctaccactt caaataagcc
    39361 catgctgttg ctgcctttta gcataatact atctgttgct tcaagatttt gtttgatagc
    39421 atcaatcaat ttattctttt ctttttcaaa atagaacaca ggtagttggg ggtatttttg
    39481 gttcaattct tcttttagtg catgcatttg ttccccataa aggaaaatca tggcgaattt
    39541 ctcaggatcg atatgttcac tcatttgcgc atgcatattt aaagactctt tccctaattc
    39601 cagcatatct gctaacacag cgattctgcg tccttttgtt ggcagatttt taaaagtatc
    39661 taataccagt cccatggctg ttggatttgc attgtagaca tcgctcaaaa tagctgcacc
    39721 atttcctgcc gttagccatt cggttctgtt ttgagtgata gaaacatggg cgagcccttg
    39781 gcgtatctca ggtatggaca gtccaaagaa gcgcccgatc gtatacgcaa tcaaagcatt
    39841 cgttacatta taacttccag gcagcgggat cgtaaatgtt tctccttcta ctacaaaatc
    39901 ggtttgtgct tttccttttg ctgtcacttc cgcatgaaca tcgccatttc caataccgaa
    39961 tgttgtgact gtttgtacta atgttgccgt taaaggtgtc agcaacggtt catctgcggg
    40021 cacaacgagt aatccgtctg atacaagtcc gtcagtgatt tccattttgg ctttagcgat
    40081 tccttctcgc gacccaagat tttctatgtg tgcttcaccg atcattgtga ttgctgcagc
    40141 ttttggttga gccataagtg aaagttccgt gatttcattg gcgtgatcca tgcccatttc
    40201 caaaacgatc ttttctgtat cttggggcat gtgcaaaatc gtgtaaggaa gaccaatatt
    40261 attgttgtaa tttccttgcg tcttatatgt tttgtatttt tcagacaaga cagacgcggt
    40321 tagatctttc gtggtggttt taccattgct tcctgtaatt gcaacgactt ctgcgcctac
    40381 tttttccaag taataggttg ccaattgctg catggctttc aaaggatctg ctacaggaat
    40441 aactggaaaa ttttttggag cttgttcaac aggcaagctc caaaatgaag cagatgctcc
    40501 gtttgtgatc gcttgctgga taaattcatg tccatctcgc gtaccagcaa gaggaacgaa
    40561 taaacttcct tcggttatct tccggctgtc aaattcgaca ttggtaatct ttgtcagtgc
    40621 agagcattcg ctgcccacgg cttgtgcaat ttcttgtatt gttagattca ttgagatctc
    40681 tcctaaaaaa ggttgtgaaa aaagcgtttg gcctaaacgg cgggttcgca tgactctcac
    40741 ttccaagtaa atttctctat tcgtatttag aagctagaat gaacgcggtt acgcaatcac
    40801 tccagcctct tctttatttc tctttattca tttttttcaa aaaaagattg acgctgatgg
    40861 tatcgattca ttcctaactg gatcagttct tcaatcaaat caccgtattt caagcccata
    40921 ttttcccata agagtgggta catactgaac tccgtaaatc ctggcataga gtttaattca
    40981 ttcaggaata attcattttt atttgtcaaa aagaaatcgc accggctcaa tccgcttcca
    41041 cctaacatcg tgtaagctaa cttcgcgtac tcttgcgctt tttgataaac ttcttctggc
    41101 acttcggctg gaatctgcat ttcgatttta ttattgatat attttgcttc ataatcatag
    41161 aatgctacgt cttttacgac ttcaccaggc aaagtcgtcc gaacgtcttc atttcctaat
    41221 acagcaactt cgatttcgcg cgcttcaatt ccttgttcaa cgattgctcg agaatcatac
    41281 tgataggctg ttgctaaagc attttgcagc tcttctcggt tttctgcttt tgtaatgccg
    41341 acactagaac ccatattcgc cggtttgaca aacatcggat aaagcaaaga accttcacat
    41401 tgatcaaata cttttttagg attttctttc cattgattct taagtactgg tacataagga
    41461 acttgcggca caccagcagc ttgtaaaata tacttggtca tgattttatc catggcacat
    41521 gcactggtca atacgcctgc gccgacataa ggcatattca atgtctctaa gaagccttgg
    41581 atcgttccat cttccccgtt tggcccatgt aaaactggaa aaacgatggc tccttcttct
    41641 ttgatttcgc ccggattgat cacttttcct gtaaagcctt cctctgtctg tccacttggg
    41701 tcccatgaaa gatgcaagac atctttgctg gcaggttttt ctgttagtaa tggacctttg
    41761 acccattgtc cttcttttgt aataaatacg agttgaactt ggtaataatt ataataaatg
    41821 gcatttaaaa ctgaaaatgc ggaaagaatg gacacttcat gctctgcgct gcgtccgcca
    41881 tatagtaaag taatcttcaa aagattgtcc tcctgtattc taaatcgttc agtgatttac
    41941 tctcatattt tagcacaaaa caaaaataaa cgaataactt atttataaaa aaacgagcca
    42001 gcttctaaaa gaacgagcgt catttatttt ttgtcagcta gattatttgt cccgtttttt
    42061 ataaaaagtt atgataaaga aaaggaggga tgatgatggc gcaaacaaat ccacgcagcc
    42121 gcttggcaga aatcattcga gtgttcatca attacaatgt agtgccaaac tttgtacagc
    42181 aaaaaaatcc cgaacaggta aaaaaagcct ttgaagaact aggccccacc ttcatcaaga
    42241 taggacagat gctttctgtt cgagaagatt tgctttcttc tgccttcact caaacattca
    42301 aaacattaca agatagtgta cctagcgaca cattttcaac ggtcaaaaag acgatcgaaa
    42361 cagaactttc tctttcatta tcagatatat tcgatgattt ttcaaaatcg ccttttgcct
    42421 ctgcttctat ggggcaggct catcgtgcca agctgaagaa cggtgacgcg gtagtcgtta
    42481 agatccaaca ccctaacatc gccgaagaga tccgacttga tcttcaacta tttgaacgag
    42541 cgatcccttt gatcaaatac attcctgaaa ctagcgtagt tgatctaaaa ggggtactcc
    42601 aagaagtaaa acgttccttg atcaatgaaa tggacttctt aaaagaaagt caaaatggcg
    42661 aacaattcta tcaaaagaac aatggctgga aagagatccg cagccccaag atttatgacg
    42721 ctttttgttc gaaaaaagtg atcgtgatgg aagaaatgtc tggtaaaaac ttgaatcatt
    42781 taatgaacgc cgaaaacaaa actgaaactt ttatcactgg aattcaaaac aagcagctga
    42841 aacaagaagt tgcaaagctt ttagttgaga actttatgaa acaggtgttt gatgatggtt
    42901 ttttccatgc tgatcctcat ccagggaatc tgctattcca tgtactaaca aaagaagaac
    42961 agacacaagc tagccgaaaa actgaaacag tccatgaaaa agagttcggt tcctttgctt
    43021 ttcgagcctc cacttctgca gaagaccctg ttgcgccata taccatcaac tacatcgatt
    43081 ttggtatgat ggggcatctt tctgcaggac ttaggcaaaa actcacacaa gccgttttag
    43141 cgctgtatac gaaagatgct tatcgaatcg aaaaagcggt ccttcgactt tgtcagcaag
    43201 aaggctcgtt tgatgaaagt cggttccacc aagaactgac aagttttctt gaacagtatt
    43261 atgattctcc tattgatgaa atcaacttgc aagaagtatt cacacatgtc gtgacgattt
    43321 gtcatcaaaa taatctgcag ttcgatcgtg atatcacgct tttgctgaaa gcatttggga
    43381 cattagaagg ggtgatccgc gtcctcgatc ctgaagtttc cctcatggaa gtcgcttcgc
    43441 cttttgctca acattatttt ctcactcatc tggatgtaga agatacattg aaacaaagcg
    43501 gattagactt attggaaggg atgaaggctg cgcctaagat ccctcaacag ctccaccatc
    43561 tattagaaat gtggacttcg ggacaaggaa aagtcaattt ggaattgaaa aagcaagaca
    43621 aattattgag caggatcgaa agtatgatca atcggcttgt tttcggtatg attttagctg
    43681 cattgatcgt agggtcctcc ctgcttgtcc aagcagcacc tgtagaaaac gcagaggtcg
    43741 tttccttatt agggatattt acgtatgcta ttgccgcatt cgtcattatt tttttggcaa
    43801 tcgatgcatt gatccaaatg tataagaaga ggaaaaagta gaaaaaggtt gtgacagaaa
    43861 gaatcagctt caagaaataa gaaggggttt tacataaatt gtttctcaaa tttttgtgaa
    43921 aattcggctt atttttcgaa gaggctactt ctgaaacacc gagaataaaa aatgtgaaaa
    43981 agctgttctg cacccagtaa taagaacaat caaaaaggga acatggaaca catttatcct
    44041 gtttccctgt tccctttttg atgtattcga gcataaataa aataccttcg ttacctacta
    44101 tcttctgtac ttggtaaacc ttagtcaatt gcctttttta acgcttgacg aaaagacata
    44161 tgatctttct ccgtaaaggg ttttggaccc tttgtcctta tgccagcttt tcttaacttt
    44221 ccactatgat aacgttgttc tagaagcgtg ccgatattcg cttcgtcaaa ttcttttcca
    44281 gagtgatgga acactcgcac acccttcggc aatacaagtg aagctaaacc ataatcttgc
    44341 gtgatcaaaa agtcattctt cttaaccata ccgacgattt tataatccgc actatcagct
    44401 ccgcggtcta cgtagacgat ttggacatga ttagcgtatt ctttggtcgt ataatggtcc
    44461 acactggtca cgataaccac gtcaagacca taggattctc ctaattttat gacatcttct
    44521 tttactggag aaccatctcc atcaatgaat agcctcattc tttttcgcct tcttccataa
    44581 atgataaaca gttactaatc cgctcagtaa aagaccaaca ttgatccatt ttgccaagat
    44641 aagcagttgt acactttcta tcggcactga ttcatggttc agcgtgatca ataatagatg
    44701 gattggctct cccatttctt ttgctaaaac aaacaaaagc gtcatcaata aaaactggtg
    44761 gatcacactc cctttatttt gagtagtata cttgacaacg atccggcgat ccattggaat
    44821 agctgtatca gtttgataat attccttatt tagccagctg cctgttacta cgtgccagaa
    44881 actaagcaga aagacaccta tcgcaaaacc atttggaatc aagaggacca ataaagaaga
    44941 actcagtcca agcaattgca tagccaatgg agaccatgtt aaacgtttca aaatccatgg
    45001 tccgactatt tttgctgcaa tcaagcctgc tgcataaggc aaatacataa aaatcgaaag
    45061 tttttctaat ccgtacagta tgccaacata aaaactgcta aataaaaaaa ggaaattccc
    45121 tagcattcca tttagaaaag taaacagatt tagccagctt ggcaacttcc attccttctt
    45181 ctggtgtcct aaaacaaaca cgaacaagat gaacagcagc aagaaaccat aaatagcaag
    45241 atcaaaaagc ctttcatcga ataacaatcg ggcatttctc aaaagaaaca ataagccaaa
    45301 gaatatcaaa aataaaacaa gctctcgcgt agctaccaga tttcttttga tgtctttgaa
    45361 atccagttcg taacgcggat aatgactaac cgtatggtag gccatgacgt aaaaaagtgt
    45421 ataaatagta aagcttgctg caatctgagt cggtgcaggc aactcaatcg cttgatacag
    45481 gagaaatagc cagatagctg caaataagta ctttttccca tccatattga taaaaccttg
    45541 cgtcttttcg tgaaaattga ccgtggtatt agcaggtaca agccatgctg cactcagccc
    45601 tagcaaaaca cttgaaaaat aaaataaagg aaaataaaat acgcctaata ttccaagcaa
    45661 tgaaccagca ccaccaatca gcagagcgat catcagcaac gtgtaactat ctagaccaaa
    45721 tcgtagactg ttgactaaaa atagtccagt catccgaaag gcataaaaca gcgtaaatgg
    45781 caaaatcgtc agccaattat gcgagtctgt tagtcccaaa aataaaagat aaggaataaa
    45841 aataaccgtg ttacttaaga taaagggcgt tgtttgtgta aaattacgcc caaatcgttt
    45901 gagtttttcg aacatgacaa tggctcctta aacagtctta taactaatct gctcttcgat
    45961 tttagcatta aaggggaaat aaaccaattt tacagaatat attcttgtaa aaaaaaatac
    46021 agaaagaagc ataagcttct ttctgcattt ttacggtact tgattactca agcagacaag
    46081 cccttctcca caaaagtctt gcccgacacc cctgcatccg gcaggtgata aagatagaaa
    46141 tcaattatcc tgtgcctcca aaatcaatta ggagctctgt aaaccagagt ccttctcgac
    46201 ccgtcgcata aatttacttt aacacagttc ctatgtttat gcgagagctt ttttcatttt
    46261 ataatcgacc atttgaaatc cttgaccgat atctgaaata agttcttctg taatctcaaa
    46321 tccttcatgc ttataaacct cgattgcttg atgattcccc tgattcactc gcaaaaagat
    46381 ctctctttcc tttgtttgcg cagcaagttc ttcataccag cgaaaaactg aagaagtcaa
    46441 tccttgacca cgtactttat ttagcaaata taatttactt aaataaattt ttccatttat
    46501 ctcttcataa gcagtgtaac cgacagtctt ttcttcgctt tttaataaaa agtaggacac
    46561 accttctgtc agttcttttt gaatctgttt ttttgactga taagttttca gcatgtaatc
    46621 cacttgtttt tgaccaatga tcggcacaaa aacttccggc catacttctt gtatgactgc
    46681 catcagctca tccagttcat tttctgttac ttttattttt tccatacacc cacgtccttt
    46741 tttacattat aaatgacctt gcgcttgatg gaataaaaag agcatgatca tgctatacac
    46801 acccaccgac cacggtttca agaaaatcac aatctgcata aattccctaa aagataattt
    46861 cgtcaaacca gctactagac agatcaagtc gtctggcgca aagggaagca gcaaggtaac
    46921 aactagtact tttttaatat ctttcgtttt tcttgtcagc aattcatcaa atttctgata
    46981 cttttttggc gataaaataa gctggacaaa ggctacgcca tagtaacgga caagcaaaaa
    47041 tccgattgct tccccaatca ctaggccaat acaactatat aaaagcccaa atccattgcc
    47101 aaacatcagc atgcccgcta ctgaggagat ccctcccggc aaaataggaa tgatcacttg
    47161 gatcacttgc aacagaacaa atatcatcac tgcatacttg ccaaattgct ggataaacgc
    47221 ttgcaaagac gtgctcgatc gaaaaatccc atgatgataa gcataaagaa tcaacacaaa
    47281 gaaaaaaaac agaccaataa tgggcatgat ctgaagacat ttttgtaaaa ctgatccttt
    47341 tttagacatc tatattcccc acatcttttt tcatttatta aaactgcctc ctt
//
LOCUS       DAHHZJ010000007        44383 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.007, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000007 DAHHZJ010000000
VERSION     DAHHZJ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.007"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(330..2711)
                     /locus_tag="NBL02_000275"
     CDS             complement(330..2711)
                     /locus_tag="NBL02_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HCD2570991.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2701..3348)
                     /gene="recU"
                     /locus_tag="NBL02_000276"
     CDS             complement(2701..3348)
                     /gene="recU"
                     /locus_tag="NBL02_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HCD2570992.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3412..3951
                     /locus_tag="NBL02_000277"
     CDS             3412..3951
                     /locus_tag="NBL02_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HCD2570993.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4041..4460
                     /gene="gpsB"
                     /locus_tag="NBL02_000278"
     CDS             4041..4460
                     /gene="gpsB"
                     /locus_tag="NBL02_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HCD2570994.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4482..4849
                     /gene="rnpB"
                     /locus_tag="NBL02_000279"
     ncRNA           4482..4849
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="NBL02_000279"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5058..6224
                     /locus_tag="NBL02_000280"
     CDS             5058..6224
                     /locus_tag="NBL02_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HCD2570995.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6270..7766
                     /locus_tag="NBL02_000281"
     CDS             6270..7766
                     /locus_tag="NBL02_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HCD2570996.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7847..8530
                     /locus_tag="NBL02_000282"
     CDS             7847..8530
                     /locus_tag="NBL02_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCD2570997.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9175..10365
                     /locus_tag="NBL02_000283"
     CDS             9175..10365
                     /locus_tag="NBL02_000283"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCD2570998.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10467..11420
                     /locus_tag="NBL02_000284"
     CDS             10467..11420
                     /locus_tag="NBL02_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCD2570999.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSGNVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11489..12838
                     /locus_tag="NBL02_000285"
     CDS             11489..12838
                     /locus_tag="NBL02_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD2571000.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(13021..13344)
                     /locus_tag="NBL02_000286"
     CDS             complement(13021..13344)
                     /locus_tag="NBL02_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571001.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13473..14636
                     /locus_tag="NBL02_000287"
     CDS             13473..14636
                     /locus_tag="NBL02_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HCD2571002.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14706..16055
                     /locus_tag="NBL02_000288"
     CDS             14706..16055
                     /locus_tag="NBL02_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCD2571003.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(16100..16531)
                     /locus_tag="NBL02_000289"
     CDS             complement(16100..16531)
                     /locus_tag="NBL02_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD2571004.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16919..19561
                     /gene="alaS"
                     /locus_tag="NBL02_000290"
     CDS             16919..19561
                     /gene="alaS"
                     /locus_tag="NBL02_000290"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCD2571005.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKEHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19761..20357
                     /locus_tag="NBL02_000291"
     CDS             19761..20357
                     /locus_tag="NBL02_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCD2571006.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20479..21627
                     /locus_tag="NBL02_000292"
     CDS             20479..21627
                     /locus_tag="NBL02_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCD2571007.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21665..22078)
                     /locus_tag="NBL02_000293"
     CDS             complement(21665..22078)
                     /locus_tag="NBL02_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HCD2571008.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            22157..22588
                     /locus_tag="NBL02_000294"
     CDS             22157..22588
                     /locus_tag="NBL02_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HCD2571009.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22687..22920)
                     /locus_tag="NBL02_000295"
     CDS             complement(22687..22920)
                     /locus_tag="NBL02_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HCD2571010.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            23103..24773
                     /locus_tag="NBL02_000296"
     CDS             23103..24773
                     /locus_tag="NBL02_000296"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HCD2571011.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            24920..25306
                     /locus_tag="NBL02_000297"
     CDS             24920..25306
                     /locus_tag="NBL02_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571012.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            25293..25991
                     /locus_tag="NBL02_000298"
     CDS             25293..25991
                     /locus_tag="NBL02_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCD2571013.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            25996..26460
                     /locus_tag="NBL02_000299"
     CDS             25996..26460
                     /locus_tag="NBL02_000299"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCD2571014.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26457..27368
                     /locus_tag="NBL02_000300"
     CDS             26457..27368
                     /locus_tag="NBL02_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCD2571015.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27603..28142
                     /gene="pyrR"
                     /locus_tag="NBL02_000301"
     CDS             27603..28142
                     /gene="pyrR"
                     /locus_tag="NBL02_000301"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HCD2571016.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            28156..29439
                     /locus_tag="NBL02_000302"
     CDS             28156..29439
                     /locus_tag="NBL02_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCD2571017.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29452..30378
                     /locus_tag="NBL02_000303"
     CDS             29452..30378
                     /locus_tag="NBL02_000303"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HCD2571018.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30397..31677
                     /locus_tag="NBL02_000304"
     CDS             30397..31677
                     /locus_tag="NBL02_000304"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCD2571019.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31682..32758
                     /gene="carA"
                     /locus_tag="NBL02_000305"
     CDS             31682..32758
                     /gene="carA"
                     /locus_tag="NBL02_000305"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCD2571020.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            32763..35948
                     /gene="carB"
                     /locus_tag="NBL02_000306"
     CDS             32763..35948
                     /gene="carB"
                     /locus_tag="NBL02_000306"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCD2571021.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            36002..36778
                     /locus_tag="NBL02_000307"
     CDS             36002..36778
                     /locus_tag="NBL02_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HCD2571022.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            36775..37707
                     /locus_tag="NBL02_000308"
     CDS             36775..37707
                     /locus_tag="NBL02_000308"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HCD2571023.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            37704..38420
                     /gene="pyrF"
                     /locus_tag="NBL02_000309"
     CDS             37704..38420
                     /gene="pyrF"
                     /locus_tag="NBL02_000309"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCD2571024.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38427..39056
                     /locus_tag="NBL02_000310"
     CDS             38427..39056
                     /locus_tag="NBL02_000310"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCD2571025.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            39044..39358
                     /locus_tag="NBL02_000311"
     CDS             39044..39358
                     /locus_tag="NBL02_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571026.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            39396..40037
                     /locus_tag="NBL02_000312"
     CDS             39396..40037
                     /locus_tag="NBL02_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCD2571027.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            40080..40772
                     /locus_tag="NBL02_000313"
     CDS             40080..40772
                     /locus_tag="NBL02_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HCD2571028.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            40769..41671
                     /locus_tag="NBL02_000314"
     CDS             40769..41671
                     /locus_tag="NBL02_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCD2571029.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(41829..43535)
                     /gene="efbA"
                     /locus_tag="NBL02_000315"
     CDS             complement(41829..43535)
                     /gene="efbA"
                     /locus_tag="NBL02_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HCD2571030.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            43725..44348
                     /locus_tag="NBL02_000316"
     CDS             43725..44348
                     /locus_tag="NBL02_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571031.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
BASE COUNT        14244 a         7805 c         9638 g        12696 t
ORIGIN      
        1 gaagccttct ttcaaaaaat tattcaaaac catatattgt attttttatt tgataataac
       61 aatgggatta ttaagaagtg gcactaaata aaagactagt gtgtatttag ccgatttaat
      121 tataaacaag taaatggata aagtatatgt aatgataaaa gaaaaatgga ttttctaatt
      181 tttaagctaa atgttagatc acacatgtat atgctggata gtcttataaa aaatcatgta
      241 atacagcttg gtacatgatt tttatttgta gctgtttttt acatagacac aaaaaagaaa
      301 ccacgcttgt agacgtggtt tcttttttac tatctgtttc cagaagctga ggtgctgctg
      361 gatggtgtat ggtcatttgc agcatccgct ccgctagttg ctggctcact ggaagaagca
      421 ggaggagcag atgaagacgg tgtcgagctt tcttcggatg aagaagaagg aaccgttgtt
      481 gaagacgctg gctcgcttgt ttctgctgtc gtgctttctt ttgaggactc agcagttgat
      541 tcgctttgac tagtagagct agagctttga gtcgttgtct ctgttgttga gcttccagga
      601 gtctggacat agctggaaga tggaatagtt gttgatggtg taattgcatt cgatctagct
      661 gtatattgat ctttgtagta cagttcaccg cccacacgaa ttaatccgct tggcatctcc
      721 cagtctgtat ttgtcacgtt agctgaaaca tactgcataa gctcgcgata aacatcagag
      781 gctacgtgtg acgattctga agtgacaggt gtcatttttt tattgtaacc tgtccacacg
      841 gaaatcgaat aatttggcgt atagcccgca aataggatat ctggatacac cccagaagaa
      901 ataccaagtt ttgcatactc gtcatctgtg taattggatg ttccggtctt accggcttga
      961 tataacccag cgatttgcgc attcgtacca gttccttcag tgatcgtatc ttttaaaatg
     1021 tctgtgatca tataagctgt ttctggagac atggctgttt ttccatctgg ctcataagtt
     1081 tcttctgttc cgtcttggaa gacgatttta ttaacatatt gaggtttgta ataagtccct
     1141 ccattggcga aagccgcata ggctgctgcc atttttaagg aagaagctcc gtattttgtc
     1201 ccatcttgtt cttccgtatt actagagatc gcattcgatt gatggatcgt actgtattcg
     1261 atcccaagat tttttaagaa agaagccact ttatctgaac cgacttcatt gaataatttg
     1321 actgctggaa cattacggga taagtaaagt gcttggcgta aagtaatggt gcccatatat
     1381 tgattatccc agtttcctac aggagtcgaa gtcccttcat agttatacgg tgcgtcagta
     1441 atagtttttc cagtagaata tttcaaatat tcaaaagctg gtccgtaatc tgtgacaggt
     1501 ttcatcgtag aaccaaaatc acgggaagta ttcactgcta agttgttgcc taaagttacg
     1561 tcttctgcga tgtgacgtcc gccaatctgg gcttttactt tcccggtatt ggtgtcgata
     1621 agggtggagg caacttgcat ctcgtcgtct gggtaagaga catactgatc cgtgttgacg
     1681 atatcatata gtttcttttg ggcgtccaga tccaaattcg tataaatctc taaaccgtct
     1741 gtgtagacat tcttatctgt tttttcttgt acttcattga tgacttcttt gacataatta
     1801 tcgacgatct tagtattgtc atcggattga gttagttctt gcagaccatc cgttaccggt
     1861 acattaacag cttgatcata ttctgtttga gagatttttt cgttttgaag catcgtatac
     1921 aaaacagtat ctcgtctttt cttcgcctga tctggataaa cataaggatc ataggcggaa
     1981 ggggcttgag gcatcccagc aagcaatgct gtttgcggta aagaaagttc tgataatttc
     2041 ttaccaaagt acatctcaga agctgtctcc atcccgtaca agccgtttga catatacact
     2101 ttatttacgt agtaggtcaa gatctcttgt ttcgattttt tttgttccaa tcttacagcc
     2161 atccacgctt cttgggcctt gcgtttcaga gtctgatcct cggcactggt ggagaagaag
     2221 gaaagtttga tcaattgctg tgtcaaagta cttccgcctt gcagtccgcc agaggtaaag
     2281 ttagagaggg cagaaccgat gatccgaata gggtcaaccc cgatgtgttt ataaaaacgg
     2341 cgatcttcta ccgacacgat cgcatcttct aatgttttcg gtaattcatt cgcagaaatt
     2401 ttttcacgct tttctgcgcc taaatcttcg aatagttcgc catcttgtgt atatagtttg
     2461 gaagatacgg tagcgtctaa ttttttatcc gtcaattcgg gcgcatcttt tgcgtaatac
     2521 cagaaaagtc ctacacctgc taaaaagagg atacagaaaa atgagagtat tcctaaaagg
     2581 atcttgataa ataatccttt tttatgtgtt ccagaggact ttccagaacc tttgccagaa
     2641 ttcttcttca cactcttttt aggagtaggc tgtttctgtc gtctagagct tctagtttgt
     2701 tcatttgcca ttcttgcttt ctccttcaat agataatgtt gataaataga agtcaaccgc
     2761 gtccaaataa ggaatccgcg gagaaatacc agttttgatc tctattccta gttcttcgat
     2821 caaagataaa ggtagtgatt tgcgtccttt ttccttttgg gcgtcccagt aagcaatcaa
     2881 atgaacacta ttcaaataaa aacagcgctt caaagacgaa aaccataaca agacaaagca
     2941 gaccccattt tgttcgaggc atgctttcat atgttcgatt tgatgggcat ggaagttttt
     3001 aaagggaaaa gatgttttgt tcttggtttc ttttgcttca aagtccaagt aaaagcctcg
     3061 atagacgcca ttatagtctg tagttgaggc ttgggtaaag taggcttcct tgatcacagc
     3121 agcacttctc ttaggatagt ctactttcac gatttggata ggggtaggct ttttatggat
     3181 aaccgccaac ccgcgggaaa gataatattt attactttca ttgattgctt cttcaaagcg
     3241 catcccccga ttgccgaatt cagtgatctg atttcttttt tcttgaattt tttcgacggg
     3301 agaggcattc ttataataag gttggccatt tggatagcgc aataccatct ttaatcacac
     3361 tcctaggcca cattatagca aaaaagcgga tagaaagaaa aggaatctga gatggataag
     3421 tcaaaagtaa tctatgtgtc tgggtatcga agtttcgaac tcggtgtttt taaagaaaat
     3481 gatcctaaaa tccaagttgt taaaaatgtt ttaaaaaaag aactccatca gttagcagaa
     3541 gaagggctgg aatgggtact tgtcagtggg aatttgggcg tcgaactatg ggctgtgcaa
     3601 atagtagcgg aattgaaaga agaatatccg gaactatctc ttggtatttt gtatcctttt
     3661 tctgattttg gtaataactg gaatgaaggg aaccaagaaa aaaaacaatt agcagaacaa
     3721 ttagcagatt atgtcgaagc tgttagccat caaccctacc aatcacctag tcagctaaag
     3781 aatcacactc gttttatact tgagcataca gtgggctgtc tgctgatcta tgatgaagaa
     3841 ttccctggaa aaacgaagta tttttttgaa gatgcacaga attaccaagc agatcatccc
     3901 tacgaaattc ggctgatcag tatggacgat cttcaaaatt ttagtgaata atcgatattg
     3961 tgtttgattt atcaactttt ttcctataca atgaaaaagg tagaaatgtt attacgtaaa
     4021 tgaaaactag aggtgtaaat atggcgaatt tggtttatag tcctaaagac attttacaac
     4081 aagaattcaa aacaaaaatg cgcggctatg atcccgttga agttgatgaa ttcttagaca
     4141 atatcatcaa agattatgaa acttacagca aagaactcct tgctttacaa gaagaaaacg
     4201 atcgattgag tgcaaaagta gctcaattat ctaaaactca aggagcagct caaacacgcg
     4261 tgcaacaaac agaggcacca aagagcgcag cagtgaccaa ttttgatatc ttaaaacgct
     4321 tgtcaaatct tgaacgtgaa gtatttggta aaaagcttga tcaacaagca agtgcagtca
     4381 aaccagcaca acctaatcca aataactaca caaatgcaga cacaagctta gatgataatg
     4441 aaaaaacacg tcaattttaa tttttgatta atgaatttgc ggttttcggg taatcgcggt
     4501 ctgacttgat caggctgagg aaagtccatg ctcgcacagg ctgtgatgcc tgtagtgttc
     4561 gtgcttagcg aaaccataag ctaaggtact ctttggagta acggcaggaa aacaagctaa
     4621 ggcttcggct atgcttaagt atccttgaaa gtgccacagt gacgaagcgg ctggggaaac
     4681 ccatccggtg gaacgcggta aacccctcga gcgagcaacc caaacaatgg taggggcgct
     4741 ttttgtctgg aagaagaaca ggacaaaaag gcagtataac tgcagataga tggttaccgc
     4801 tttgtttttt ctccctggaa aagcaaagta cagaacatgg cttatagaaa accgcaaaca
     4861 tcagggaatt aggtcgtgac aaaagtaatg cgttataaga aacaagaaaa acatttgaaa
     4921 gctatttttc aatacttcag atgaatcgtt cttattacaa aacaaacttt tgtatcaccg
     4981 gttattcgga atttgagtaa aagtgtgggg taggactgat gtcccacctc ctttttttat
     5041 aaataaggag attgtgtatg gaaaaaaaca ggaagttcaa cctcgtcgca actgcggcaa
     5101 gcgggctaga agcactggtt ggtaaagaat taagagatct tggaatcgaa tgccaagttg
     5161 agaacggacg tgcacgtttt gaaggaacaa tggaaacgat cgctacagct aatttatggt
     5221 tacgtacagc agatcgagta aaaatcgtgg ttggggaatt cgatgcgtat acgtttgatg
     5281 aattgtttga aaacgtaaaa gcattgcctt gggaagattt tcttccacta gatgcagctt
     5341 ttcctgtagc tggaaaatcg atcaagtcga aactatatag cacacctgat tgccaagcga
     5401 tcacaaaaaa agcaatcgtt gaacgtctcc gtacctatta tcaccgtcca gcttctgtac
     5461 cactgacgga aactggcgca cattttcagt tggaagtagc tttgctgaaa gaccatgtga
     5521 tggtaactct ggatacgaca ggaccaagtt tattcaaacg tggttatcgc ttagaaaaag
     5581 gtggagcacc attgaaagaa aacatggcag cagcacttat tatgttgaca aattggcgaa
     5641 aagaccgtcc attttatgat cctgtatgtg gttcaggtac gctttgtatt gaagcagcct
     5701 taataggcca taatatcgca ccaggattca atcgtgagtt tgcttgcgaa tcttgggatt
     5761 ggttcagtca agaaatcatg gaaaatgttc gatcagcagc cgaagaaaaa gcagattatg
     5821 atattgaatt agatattact ggttcagata tcaatggacg gatgattgaa attgccaaag
     5881 ccaatgcaga agaaatcgga ttaggctctt cgattacctt taagcaacaa gcagtcaaag
     5941 acttcaaaac ggataaagaa tacggggtca tcgtggctaa tccgccttac ggagaacgtc
     6001 taggagaaga agaaactgtt cggaaattat ataaagaaat gggcgatgta ttccgtccat
     6061 taaaaacatg gagtaaatat atcttgacta gcgacttagc gtttgaggaa ttttacggac
     6121 aaaaagcaac caaaaaaaga aaattgtaca atggtgcatt gcgtacggat ctattccagt
     6181 attggggaac tcgtccgcct agaaaaaaag agaaagacga ataatcttac taattgattc
     6241 attcaattgg atgtttggag gaatagggaa tgaaagaaaa agagttttta caagaagtaa
     6301 aagaaatcaa tcttctacaa caagctttag gaatcctcga ttgggataca caaacaggta
     6361 tgcctgaaaa agctagtgca taccgaagtg aagtagacag ctatctttac agcatttatt
     6421 tttcaaaaaa aatcggtcca aaaatcaaag aagccattcg ctatttttct gaacatccgg
     6481 aagaattgtc ggaagttgga actgccgttt ttgccaaagt aaaagaagag tatgatttgg
     6541 aacatcgcgt accggaagat cttatgagaa aatatacagc tgccgtttct tcagcacacg
     6601 gacaatggca aaaagctaga gaaacacaac aattttctga ttttcaacac gcattgaatc
     6661 aaaatattga attaacaaaa cagttgatac cttactggag aaaagatgaa aagacgaatt
     6721 atgatgtttt gctgaatcaa tacgaacccg ggatgactgt cgaaatcctt gataaggtat
     6781 ttgatcagct taaagaagga attctttcga tccgccgtac gctccaagaa aaagggactg
     6841 aacctgaaac agacttttta aatcggagaa tgacgaaaaa acaacagaag agatttgtag
     6901 tgaaagtaat cgaacaactt ggttatgatt ttagtcgtgg tcgtttagat gacactgtcc
     6961 acccatttat gactggaatt aatccgaatg atgcacgaat cacgacacga tggaacgaaa
     7021 cagatttcaa aatggctgtt tttggagtga tccacgaggc tggtcatggt atgtatgaac
     7081 aagacatcga tgataaatat gcgtatacac cggtttatga aggtacatca atgggtatcc
     7141 atgaatcaca atcgttgttt aacgagatca ttattggaag tagtcgcagc ttctggaaaa
     7201 agcaatatcc tttctttcaa gaatgtgcag aaggaacatt cgatgatatt ccttttgaca
     7261 aattttatcg ttcattgaac catacaaaat caagtttggt tcgtatcgag gcagacagtt
     7321 tgacttaccc tctccatatc atcattcgtt atgaaatcga aaaaatgatt ttcaatgaag
     7381 gggtagatgt tgctgaacta cctgaaatat ggaatcaaaa atatgaagag tatctgggaa
     7441 tacgaccaac aaatgatgtg gaaggcatcc tacaagacgt tcattggtca ggtgcaagct
     7501 ttggttattt tccctcttat gcattagggt acatgtatgc ggcacaatta cttcattctt
     7561 tgcagcaaga aatagatgtg gaaaatgttc tagcgtcaga cgattattct ccaatcaaaa
     7621 actggatgtc agagcatatc catcaatatg gtgcatctag aaaaccaaat caattaatca
     7681 tggatgcaac acatgaaccg cttaatccgc aatacctgat cgactatatg aaagacctgt
     7741 attttaatgt atatcaggta aaataaaata caatttacaa agatagctga aattcatcta
     7801 atctatagat gttttcagct atttacttaa gaaagaagtg gaacgaatgg ataaattaca
     7861 agtgatcgct gattatagta tggaccaatt agcgcatgat caaacaggac atgggagcga
     7921 tcataccaaa agagttgtca agttagctga aaaaattttg gagacggaac cgcaggcaga
     7981 ccgctttgtg acgcttgcag cagcttacct acatgatacg attgatgata aagtcgttaa
     8041 aaatgagaac gaggcaaagc aacagcttcg tgtattcctt cgctctttgc caatagtaga
     8101 ggaacagatc tcgatgattt tttccattat cgaaaatatg tccttttcta aaaatctctc
     8161 tgaagcggcc gaactatctc tggaaggtaa gatcgttcaa gacgctgatc gtatcgaagc
     8221 attaggagca atcggcattt tgcgtactgc ttatttcggc ggtggacatg ggcacccgat
     8281 ttttgatcct gaactgtatc cgcaaacatt caaggacaaa gaaaattacc gaaaaggcac
     8341 gacagtgatc aatcattttt atgaaaaact gttcttgctg tctgataaaa tgaatactga
     8401 ttacggatat gaagaagcca aacgaagaga aaccttcatg cgtgaattct tagaagaatt
     8461 ttttctggaa tggtctattg acgatgaaaa ttttactcca gacaattgga tcaaaaataa
     8521 caaaaaatga tggaggttgt gacagaagtg gtcagctcca agatatttcg gatcattttc
     8581 cgtaaaagtt acttctggaa cactgtttac caagaggtcg tgagcctgcc gttcagctcc
     8641 gagtcacaag gaacaatttc aaaaaatagc ttctcgtgtt tgatgaatat tgtaacttgt
     8701 gattcggaat tggcaggaga acaccgttta ttcggaagaa taggactgtg acaagacttt
     8761 tgtcacagtc cttttttcga attcaacaag taaaactttt aagaaatatt cagagagtaa
     8821 aattaaaaat tgctgataaa cgaagatgga aattttatgt atttttattg gtctatgcaa
     8881 taaataaatt aaatattcag aatacaattg attttaacga aagggcttct atcaatggtt
     8941 gtgttgtatt tatctgccca ggtagagtaa aatagatcat tatctaaaag gtaattgttc
     9001 atactatata agtcatttga aagtaaaaag atactgtaaa taaaaaatgt ggttaaataa
     9061 ttctcataat cagtgtaagc gttatcaaaa atatagcata cttgctttgt agatgaatga
     9121 cacgatatat gtttggtatg ttctgcaaga cagatgaata tggaggtgga gacaatgtct
     9181 tccaaagaac taacaatgta tttagagaaa gaattggatc aactgaaaga aaaggggtta
     9241 tacaatacga tcgacatact agaaagtgaa aatggtgctt gtatcatcgt agacggaaaa
     9301 aagatgatca atctagcatc caacaattat cttggatttg ccaatcgcga agaattgaaa
     9361 aaagcatgta ttgaagcaac cgaaacttat ggtgtaggag caggagcagt ccgaacgatc
     9421 aatggttcgc tgaaaatcca tcagcaatta gaagaaaaaa tcgcagaatt caaagggaca
     9481 gaagcagcaa tcgcctttca atctggattc aactgcaata tgggcgctat ctctgcagta
     9541 atgacaaaag aagatgctat cttatcggat gaattaaacc atgcatctat tattgacggt
     9601 tgccgcttat ccggagcaaa aatcatccga attaaacatc aagatatgaa agaccttgag
     9661 aaaaaagcaa aggaagcgat agaaagtaag aaatataaaa aaatcatgta tatcacagat
     9721 ggtgttttct ccatggatgg agacattgca cgattgccgg aaattattcc gattgttgaa
     9781 aaatatggac tgatcaccta cgtcgatgat gcgcacggct caggcgtaac tggaaaaggt
     9841 gcaggaaccg tcaagcattt tggattaagc gacaaaatcg atatgcaaat gggcacgtta
     9901 tcgaaagcta taggtgttgt tgggggttat gtagctggat cgaaaacact aatcgactgg
     9961 ttgaaagcta gaagtcggcc attcctattt tctacttcgc taactccagg tgcagctgca
    10021 gctgcattgg cttcgattac tttgatgcaa gaacatccgg aacttgtcga aaaagtttgg
    10081 gaaaatgcta attattttaa agaagaattg aaaaaagtag gctataacat cggtatgtct
    10141 gaaacaccta ttactccagt cattttggga gacgaaaaag tgactcagac tttctctaag
    10201 aagctgatcg aacatgggat ttatgctaaa ccaatcgttt atccaactgt gcctcttggg
    10261 acaggcagga tcagaaatat gccgactgcg gaacatacga gggaaatgct tgatgaagca
    10321 gtagcagttt atcaaaaaat aggaaaagaa atggaaatta tttaactaaa tttagttttt
    10381 tcgtctattt aaaattagag aacatgttca cgcatatatt gttaatcatc tgaagatagt
    10441 agtaaaaaag atcagggagg atcatcatga aaaaagtatt ggtgacagga tgtttaggac
    10501 aaattggttc agagttgatc acacggctac gtcaagaact aggcagtgga aatgtcattg
    10561 cgacagatat acgcatgcct gaagtcattg agaaaaacga tatctttgag atcctagatg
    10621 taatggatta cgaaaaaatg cgcagcatag cagaacaata tcaagtggac acattgattc
    10681 atttggctgc tcttttgtca gctgttgcag aagcaaaacc acaattagcc tggaatttga
    10741 acgtgactgg gctggtcaat gcattggaag tcgctagaga attccagctg aaatttttta
    10801 cgccaagttc aatcggcgcg tttggtccaa atactccaaa agatcatacc ccacaagata
    10861 ctcttcagcg tcctacaacc atgtatgggg taacgaaggt agctggagaa ctactatgtg
    10921 attattatta tgtgaaatat ggcgtagata ctcgtggtgt ccgtttccct ggattgattt
    10981 cttatgcaac cttgccagga ggcggtacga cagactacgc agtcgatatt tattattcag
    11041 caatcaaaga aggaaagtac actagtttca ttgatcaagg tacatcaatg gatatgatgt
    11101 atatgccaga tgcgatcgat gccattataa aactcatgaa tgcagatcct gatcgattgg
    11161 ttcatcgaaa tgccttcaat atatcggcga tgtcttttga cccggaagag atcaaagcat
    11221 ctattcaaaa aatcatacca gattttgaaa tggattatga tgtggatcct gtacgtcagt
    11281 caatagcaga atcttggcca aattcactag atgtttcttg tgcgcaagag gaatggggct
    11341 tttcgccgaa atacgactta gatgtgatga caaaggatat gttggaaaaa ctgaaagaaa
    11401 aattggtaga gacggtataa aacaagaacc acatccaaaa agttttttat acttttggga
    11461 ttggctatgg tatgaaaggg gacaagggat ggaagaatat caagccacgc cagtaaaaga
    11521 agtaaaaata gaaaatgaat taaaacgtac gatgggattt tttactgctt tatcgactgt
    11581 tatgggcaca gtgatcggtg caggggtatt ttttaaagcc gcaagcgtag cagaagttac
    11641 aggatccgcc agtttgcata tgttttcttg gtttctcggt ggtatgattt ctgtctgtgc
    11701 aggactaaca ggagctgaac tggcagctgc tattccagaa acaggaggga tgattaaata
    11761 tatcgaacgg atttacggaa atacagcagc ttttttattg ggctgggcgc aagtcgtgat
    11821 ttattttcca gcaaatgtag cagccttgtc gatcattttc ggtacacagt tcgtcaatct
    11881 atttggtctt tcccagtcaa tgattgttcc cgtagctgtt acagctgctg tatcgatcct
    11941 gttgatcaat tttttaggtt ctaaagctgg aggggctttt cagtcgatta cgcttgtctg
    12001 caagcttatt cctttgtttg tgatcgtcat tttcggtttg ttccgacaag aaggagtaga
    12061 cttccaactg tttccgatcc aagcaggaga aaatctttca tttttctcag ccttgggagc
    12121 gggcttgcta gcaacgatgt tcgcatacga cggttggata cacgtaggaa atatttcggg
    12181 agaattgaaa aagccggcga aagatttgcc aaaagcaatt tctttaggaa tcatcggaat
    12241 catgatcgtt tatttattag taaatgcggt gttcttaaga accgcatcta tcgacggagt
    12301 cgcaggaaac agcaatgcgg caagtgacgt tgcaaaaatg atttttggcg gcttcggtgg
    12361 aagactggtc acagttggta tcttgatttc tgtttatgga accatcaatg gctataccct
    12421 tactggaatg cgtctccctt atgttatggc aaaagaaaac aacctgcctt ttagcaagct
    12481 ctttgctaaa cttcatgata aaactaaagt tcctgtagcc gcaggtatct tagagctagt
    12541 catcgctatt ggcatgatga tgatcggtgg ttttgataca ctaacggata tgctgatctt
    12601 tgtcatttgg attttttata cgatggtctt tgttggtgtc attcttcttc gtaaaaaaga
    12661 acctgatttg tttcgaccat acaaagttcc aatgtatccg tttattccac tagtagcaat
    12721 cattggaggg acttttatcg tatcaagcac gcttatcact cagacctttt tagcttcgat
    12781 ggggatagta ttgacacttg ctggtatccc gatttatctc tatttaaaac gtagatagct
    12841 aaaacattcg aattttatta gaactgcact tctctgccaa acaaaatatt ttatttaaat
    12901 gaggagtacg tggaagaaat gtcaaaagta ctcgttaatt ttgatcacgt aaatgtcgtt
    12961 ttgtctacga tgaaatagca aagtaaaaga cagaaatcgg gctcaaccga tttctgtctt
    13021 ttactttgat ttgcgtaact gaactattcg gcgtacgccg cgataaatat ttaataaaac
    13081 caagcaacca gaaatgaaaa tgggaagcca aatcagatag acagggtagc caagcacttt
    13141 agacaaagca tgtggagtca ttttatccat ttcaagaagt gcttgctgcc aagtctgaag
    13201 attgaagact agcatgaaac ccgaaataat caggagaata aatcctaaat aatgaattgt
    13261 agttcttttt cttacataag cgatcgtagc ataaaggaac agtaaaaaaa taggtacgcc
    13321 aaaaacaaag tacatattca gcatctcgac accccctttg tgtttatctt actttacttt
    13381 gaaagaaatg agaaataata gcctcgttac ttgcttgaaa cagcaaagga ctgctaagat
    13441 aagagagata caaagaatca aggaggagtc atatggcaag ttatatcgaa ccgattcgtt
    13501 ttgcgctgat tgtttttcca tttttagcct tagcgatctc atctgttttt tttatttatg
    13561 aatatcgaaa gtatggaaca tttctagtaa caagggcagt tattttgtat tctttcgttt
    13621 tttatttatt gtgtgcgtat tttttggtga tcttaccact tccggcaaag gagacggttg
    13681 cccaaatgac agggccaaag atggagctgc atttatttgc ttcatggcaa catttcagag
    13741 acaacacggt attggtcttg tctgaaccta gtacctacct gccggcaatg aagcagagtg
    13801 tatttttgga accagctttt aatgttttat tactcatacc atttggcatt tatttaagat
    13861 attatttccg gctttctttt atgaaaacga tgattgctag tttctgcctt tctctatttt
    13921 ttgaattgac gcagctaagt ggactttatt ttatctaccc aagaccatac cgcttatttg
    13981 atgtgaatga tttagtgaca aatacattgg gtggaatcat tggttttgtc gttacgccgt
    14041 tatttacatt tatgcttcct acaagaagac gaatggatga actttcttat gaaaaaggaa
    14101 agactgtctc attgactcga cgcttggtcg cttatttgat cgactggaca tttttatctc
    14161 ttattaattt ggtagcggcg attttcttta aaattgcgtt cgattcaggg gcagttactt
    14221 ctcatatctg gtggctttta ttagaagtag gggtatactt tatgattgta ccgtttataa
    14281 ccaatggatg gacacttggc aaaaaaattg tgcgtatccg tttggtggaa gaagaccgcg
    14341 aacgaatttc tttaaaagca ttgatgatcc gctacgggta tctgtacttt cttttttatg
    14401 gtttaagctg gttggggcag ttgcttcagc aaatcatgaa ttcgacaacg aatacggtgt
    14461 tgcagatgag tgcagtattc atttctctcg gtgtcttgtt actacaaggt atttttattt
    14521 taaatatctt gctatcattt ttgagaaaaa aacggattct gttttatgaa aaagctagtc
    14581 acacatatac tgttagcacg attggccaaa caaaggatga tgattctcga aattagctga
    14641 gcaaaaacgc tttgtctgtg ttataatgcc aaagtcgtga taatttaaag agaagaggtg
    14701 cacgaatgaa taattttgat atattccgct tcaaaccgtt tatcaatgaa gcgttaaccg
    14761 caaagggctt caaacagccc acagaggtcc aggaacgttt gattccttta attgcaaaag
    14821 gaaaaaatat cattgggcaa tctcaaactg gtacaggaaa aacacatacg tttttgttgc
    14881 ctttgtttga aaaaatcaat gcagaaaaag aggaagtaca agttgttatt acagctccaa
    14941 gtagggaact agctgcacag atctatgaag cagctacaca aatcgctaaa ttttctacac
    15001 cggagatccg tgtctcgaat tttgtcggcg gtacagacaa acagcgtcaa ttaaatcgtc
    15061 tgcatcacca acagccacag gttgtagtag gcacgcctgg tagaatatta gacatgatga
    15121 atgaacaagc gctgaagatt cacaccgcat ttgcatttgt tgtcgatgaa gcggatatga
    15181 cattggatat gggtttttta aatgaagtag accaaattgc aagtcgatta ccagaaaagc
    15241 tgcagttttt agttttttct gccactattc cagaaaaact tcgtccattc ttacgaaaat
    15301 acatggaaaa tccaatcatt gaagaaatca aaccaaaagc agttatttct gaagacattg
    15361 aaaattggct gatttcaaca aaaggtaaag atgagaatca aatgatttat caactgctga
    15421 caattggtca tccttattta gcaatcgtat ttgccaatac aaaacagcat gtggatgaaa
    15481 ttgccgatta cttgaaaaac caaggcttga aagtagcaaa gattcatgga gacatcaccc
    15541 caagagagag aaaacgagtg atgcgacaag tacagaatct ggagtatcaa tatgttgtag
    15601 cgactgattt agctgctaga gggatcgata tcgaaggtgt ctctcatgtt attaatgcag
    15661 aaattccaga agatctagac ttctttatcc atcgtgtcgg acgtactgga cgtaatggct
    15721 taaaaggaac ggccattacg ttatattcgc caaatgatga gcaagccatt gatgcgatag
    15781 aaaaattggg cgtctcattt gaaccaaaag aaatcaaaaa cggggaaatc gtcaaaacat
    15841 acgatcgtaa tagacgtgcc aaacgagaaa aatcaaaaga tgtattagat cccacgctga
    15901 ttggtcttgt gaagaagaaa aagaaaaaga tcaagcctgg ttataagaaa aaaatcgatt
    15961 gggcaattgc agaaaaaaat aaaaaagaac gcaaaatcga acgcagacag caaagccgta
    16021 ctgctcgtaa aaataagaaa aattcacata aataaaaaac aagaggttgt gacaaaaggc
    16081 gtgtcacaat ctcttgtttt taagctagac tttttttcat tccgatacaa agtacttcat
    16141 cttcaataaa aggttctgtg caagtatgat acccttgttt ttcataaaaa tctacagccg
    16201 tcatttcagc tactacataa ctagtctgac aaccttctga aaccgcccgt ttttcagcag
    16261 cttcaagcaa ctgaccgcct aacccttttc ctcggtattc ttttgctacg caaaatcgtt
    16321 ccggcttgaa gcaagtaggt gttttctttt cataacgcaa agtagctact ggtctttttt
    16381 gatcaagaaa tagataatag gtttgttgcg ggatatcttt tccgtcgaat tctagttctg
    16441 caggaatccc ttgttctaat acaaaaactt gatatctcaa aaagcagcta gctgcgcgta
    16501 tccattcatc cgctccaact atttttttca tcatattcct ccttgtcatg aatagcagag
    16561 atgtattgat tcctttattc ttatcgtacc aaaaatcgtg gagtatgacc aaaaggaaat
    16621 gaaaataaaa attgagatat tgacagtgat caatagaatg cgtatactaa ataagtacga
    16681 aaaggatatg atttgttttt taagactcac agaaaattct ttcttggctg aaaaaagaat
    16741 agtcactaat caaattgctc cctttttcta ttttggtaga aataccgacg cagctcagcg
    16801 ttatcgagtt taagaggtaa tgattacaca tcattgcaat caaggtggta ccgcgagttt
    16861 tcgtccttgg ctgtgatggt gtttttttgt atttaattta tagaaagcag gtaatgacat
    16921 gaaacaatta agtagtgcac aagttcgaca aatgtttctt gatttcttcc aatctaaaga
    16981 acatacgatt gaacctagtg cgtctttgat ccccgtgaac gatccaacct tattgtggat
    17041 caactctggt gttgcaacat tgaaaaaata ttttgatggt tcagttgtgc cagaaaatcc
    17101 aagaatcaca aatgcccaaa aatctattcg aacaaatgat atcgaaaatg tcggtaaaac
    17161 agcacggcac catacgatgt ttgagatgct gggtaatttt tcgattggtg actatttcaa
    17221 aaatgaagcc atccactggg catgggaatt tttgactgat ccaaaatgga tggcattcga
    17281 tccagaaaaa ctttatgtaa cagtttatcc aaaagataca gaagcaaaac gcatctggca
    17341 tgaagaagtt ggcctgccac tagatcacat tattgatgta gaagacaact tctgggatat
    17401 cggtgcaggc ccatgtggtc cggatacgga aattttctat gaccgtggag aagcttataa
    17461 cgatttagca gaagacgatc ctgaaaacta tccaggagga gaaaatgaac gttatttaga
    17521 aatctggaac ttagtatttt cagaattcaa ccataagcct gataacacat ttgaaccact
    17581 tcctcataaa aacattgata cggggatggg attggaacgc atggtctcaa tcgttcaaga
    17641 tgcgtcaacg aactttgaga cagacttgtt tatgcctatt atccacgccg tagaaaagct
    17701 aagcaaacaa gtaaaatatg gtgaaaaccc agtaacagat atttcttaca aagtaatcgc
    17761 agatcatatt cgcgcccttt catttgctat tggcgacggt gcattgccat cgaatgaagg
    17821 gagagggtat gtcttacgcc gtttgcttcg tcgtgctgtc atgcatggga aaaaattagg
    17881 tattgaacaa gcattcttat atcgtttagt ccctgttgtt ggggaaatca tggtaagtta
    17941 ttatcctgaa gtacttcaac aaaaagaatt tattgaaaaa gttatccgta cagaagaaga
    18001 acgtttcaat gaaacaatca atgaaggttt ggaaatctta aatcaagtga tccaagaagt
    18061 aaaaaacgaa aacgggacga ctctagcagg aaaagacatc ttcaaactat acgacacata
    18121 tggcttcccg gtagaattaa cggaagaggt ggcagaagat gaaggactga aagttgatca
    18181 tgctggtttt gaaaaagaaa tggatgcaca acgcgaacgt gctcgctctg cccgcagtac
    18241 tgaaaaatca atgggggtac aatctgcttt attgacagat atcaaagtag aaagtaaatt
    18301 cattggatac acagaacaag aatcagatag tcaattattg gtcatcgtta aaggtgagtc
    18361 tcttttgaat gaagtcgcag aaggtgaagc tcaaatgatc tttgatcaaa cacctttcta
    18421 tgcagaaatg ggtgggcaag tagcggatca aggatggatc atgaatgaag taggcgaaat
    18481 cgtagcagtt gttacagatg ttcaaaaagc gcctaatggt caatttatgc acaccgtaga
    18541 agtaaaagca ccaatgagtg aaggacaaac ctatcatcta ttgatcgatc aatcgatgcg
    18601 caatcggatc atcaaaaacc atacagcaac ccatctactg cacaaggcat tgaaagaagt
    18661 acttggcgag catgcaaatc aagctggttc tttagtcgct cctggacatt tacgattcga
    18721 ctttacccac tttggtcaaa tcacttcaga tgaattagct gaaatggaaa gaatcgtcaa
    18781 cgaaaaaatc tgggaagcta tcccagttga aacaattgaa acagatatcg atacagccaa
    18841 aaacatgggt gcaatggctt tgtttggtga aaaatacgga catgacgttc gagtagtaaa
    18901 tattggcggc tggtcaatcg aactgtgcgg aggcacacat gtagcaaata cagaaaacat
    18961 cggaatcttc aaaatcgttt ctgaatcagg aattggtgca ggcgtgcgac gaatcgaggc
    19021 tgtaacaagt aaagaagcgt acgaactact tgaagaagaa gaaaaacaac taaaacaagt
    19081 agcaacaatt gttaaatcac ctcaattgaa agaagttgtt tcaaaagcgg aacaattgca
    19141 acagcaagta agagagctac aaaaggaaaa tgaacaatta gctagcaagt tagcgaatca
    19201 acaagctggt gatgtattca aagatccaga aacgatcaat ggaaaaacaa tcattgcagc
    19261 acaagtaaaa gtgaaagata tgaatcagct gcgtcaatta gctgaccaat ggaaacaaaa
    19321 aaatatttca gatattcttg ttttagcaaa cgaacaagaa ggaaaagtca gtctgctagc
    19381 ggcaatgacg aaagaatcaa atgaagctgg actaaaagct ggcgacttga ttaaagcaat
    19441 cgcaccgaaa gtaggcggtg gcggtggtgg acgtccagac atggcacaag ctggcgggaa
    19501 aaatcctgct ggtattccag atgcacttaa agctgcgaaa gaatgggttg cgcaacaata
    19561 gggcgtttac gtttgacaga aaaacaaaag ttcaaacatt gaacgaaaca tgattatggg
    19621 ggcgaagaca aacaagtctt gccctcatac ttttttcaat tggatggcgc atctaatcaa
    19681 atggtcggat tcggatggtc tttagttgac gaaagtgaca gaaattttta cgattagtat
    19741 aaataaaaag gggtgaaaaa atgaatagta gaaataagac ctttcgacta gtcttacgtg
    19801 cgatcttgtt agcaatcatc atcgtacaag cgatggttcc ttggctgggg tttattccat
    19861 taggattcat tagtttaaca attatccata tcacagtaat tattgctgca gtagtattag
    19921 gcccaaaaga tggcatggtg attggcttgt tttggggaat cgctacaatt gttcgtgcct
    19981 atgccatgcc aacaacgcca tttgatacat tagtgttcac gaatccgatt atctcggtag
    20041 ttcccagagt acttgttggc ttagtagcag gcttggtttt ccactggatt tatcaaaaaa
    20101 ataaatcaat aacgatcagc tcagcctttg caggggtact gggttcattg gtcaatacag
    20161 tcttagtgtt aggattcatg ggattatttt atacaggtgc aacggcagat gcctatggtg
    20221 tagactctgc tcttttattt aaaaccttag caggggttgc agcaatcaat gggataccag
    20281 aagcaatagg tgcgggaatc ataacaccat tactagcaaa agcgttattt gcagcaacac
    20341 cgttgaaacc agaataatga ccatctttta atagtaatag gacgcacgat agatagaaaa
    20401 aggaagagag gagaggaaaa caatcgtcct ctccatcttt ttacctgagc aacaagcaaa
    20461 ctagaagggg taaaaaactt ggctaagaaa caagaagaaa aaaagaaaca aataccgcaa
    20521 cagcggacaa aaaaaggagt actagcgggt atcttaggat tggttacgaa tctgatcttg
    20581 tttgtctcta agctaatgat cgggcttttt tcaggaagtg tctccattat ggctgatgca
    20641 gtgaacagtc tatcagatac tgcttcatcc atattgactt tgatcggatt taaaattgct
    20701 gcaaagccag cagatcagga acatccttat ggacacgaac gcttcgaata tatcagtggg
    20761 ttatttgttt cgatcatcat tacttatgta ggatttcaat ttttagagtc ttctgctgaa
    20821 aaaatcatcc atccagaaaa tgtctcttta acacccatcg tcttttttgt cctaatcatt
    20881 tccatctttt tgaaatttgc acaaggcaaa atgtatcagg ttattgcgca atccattgat
    20941 tctgaaacat tgagagctac agcaacagat agttataatg atgtctttac tacactggct
    21001 gtattgcttt cagcagcaat cgaatgggta actggatggc gtattgatgg atatataggt
    21061 tttgctttag cattatatat cctttatagt ggattgacga tgcttcgaaa ttttgtatac
    21121 gaactattag gaagtcgccc aacgccagca gagattgaag aaatggaaga gcatttaaat
    21181 gcttgcgatg atatacttgg ctatcatgat ttattggtgc ataactatgg acccaataaa
    21241 cgctttgcat cagtccacat cgaagtagat gaatcaatga atttgaatgt ggctcataag
    21301 attattgatc tgatagaaaa agactttaaa aagtctttgg atgtggaact tgtctgtcac
    21361 ttggatccaa tgcctgttga taacgaaaaa tactataata tcttgcggca gttaaagcaa
    21421 attcttcttg aaacaggtac agggcttaat attcatgatt ttagagtaat tcatcaagga
    21481 aaaacgctgc aatttgatgt agtggtccct gagaaattcc agtttgttga tacagaacta
    21541 gaaagaatct tacgtagtca aatagaagaa aaaatcggag attaccaatt ggaaattacg
    21601 tttgatcaca attatctttt atattaaatg agaaactata aaaagactcc ttaaagtttt
    21661 taatttaaag agtctttttt tcttttaatt gtttatggag agcctgacgt gcaagattat
    21721 ctgctccttt atttttgttt tcgggcaacc attgcaggat caataaagaa aattcggata
    21781 gaagttcttg gattctttga aggtagggaa ggaagagctc gttcttcgtt gcgtttttgt
    21841 cgaccgtaga aactagtgtt ttgctgtctg aaaaacagaa aatcgtttca ttctgtaagt
    21901 cgtttttctt tagaaattct aaagttttta aaaagacttc gaactctgct tgatggtttg
    21961 tccctataga aagcgggata gatagctgtt cttgtttacc ttgatagaca atcaagattc
    22021 cacctccact aggaccaggg ttgccttttg tagaggcatc aatgtatact tttaacataa
    22081 aaagagattc tcctttttgt tcaaactaat gttatggtaa gattatatca ttactgaatc
    22141 ggtgagaggt tagagaatga aaaaaaatat tcgatggcag cctgaactgg cacatacgat
    22201 catctattgg tcattgacct tgatcattct gttttttagt ctgattcttt ctttagaaaa
    22261 cacaggtcct tattggaaaa gcaattttgt tttaggtatt tttcttgttt ttctatttct
    22321 aggtttgcgg cgttctttta ttttaaaatc aaactctgtg attatacgtt atgctgcttt
    22381 ttggaaagac cgagaaatat ctgtcggaca gattcgatct ttgacgccaa ctaatactgg
    22441 ggtgcaaatg atgttgcaag atgagaaagc cccatctgtt ttcatgatgc gaaaaaaaga
    22501 tcaagtagtc ctttttgatc attttgaaaa aaacggaccc aaatcagaat caattcctta
    22561 tccttatacg aaaaaaagaa aaagctaatt ttaaaaatca gaacctaaaa aaacgaattt
    22621 ttacaggaaa agaaaagagt gagacgtacg gttcatcccg tttagcgtct cactctattt
    22681 gtttatctat tcatgcatac tagtatcggt agtctgaacg ttgcctttct tgacatgggc
    22741 agcttgtgtt cctcgattcc cttcgatgat ctcaaattcg accacttgat tttcttccaa
    22801 agttttgaat ccctcttctt caatcgcagt aaaatgtaca aaaatttctt ctgtgtcatc
    22861 ataactgata aagccgtatc cttttttgtt gtcaaaccat tttacaattc ctgtggtcaa
    22921 agcactcacg acctttctaa agtctgatca tagccttcat tataaagagt atttataagt
    22981 acgtcaaata aattaaaaac ctgtaatttt atgttttttt tctgaataaa aaaaggtata
    23041 attggcatat cttaacaaag aagatatttt gttgaataaa gttcgtgaaa aggggtaatc
    23101 aaatgaagaa tgatctgcaa atttcacaag aaacagagct agaaccgatt gttgaagtgg
    23161 ctagtaaatt agatcttaag gaagacgatt tggaactgta tgggaaatat aaggcgaaaa
    23221 tcaatttttc tggaatcaat cgatcaaagg ataacgctga ggggcatttg atcttagtta
    23281 cttccatcaa tccaacacca gctggcgaag gaaagtcaac gatcactgta gggttaggag
    23341 acgcgctcaa taaaatcaat aaaaaatctg ttattgcatt gcgagaaccc tcacttggtc
    23401 ctgtgatggg aatcaaaggt ggtgctgcag gcggaggata tgcacaggta ttgcctatgg
    23461 aagatatcaa cttgcatttt actggtgaca tgcatgctat cacaacagct aataatgcat
    23521 tatctgctct tttagataat catattcatc aaggaaatga attgaacatc gatgcccgcc
    23581 gagtgatttg gaaacgggta gtcgatctca atgatcgtga actgcgtaaa gtaattgttg
    23641 gcttgggtgg gccaatccaa ggtgttccaa gagaagatgg gtttgatatc acagttgcca
    23701 gcgaaatcat ggctatcttg tgtcttgcgg cagaccttga agatttaaag gctcgtcttg
    23761 caaggatcgt tgtcggctat acatttgacc gtcagcctgt gactgctgga gacttgaaag
    23821 cagaaggcgc tctggcatta ctgctgaaag atgcaatcaa gccgaatctt gtacaaacaa
    23881 tttacggaac accagctttt gtccacgggg gaccgtttgc caatatcgct catggctgca
    23941 atagtatttt agctaccaag acagcattga gattagcaga ttacgttgtc acagaggcag
    24001 gatttggtgc agatcttggc ggagagaaat ttttagatat caaagtacct aacttgaaaa
    24061 aagcgccgga tgctgtggtg attgtcgcca ctgtccgtgc attgaaaatg catggcggaa
    24121 tgaaaaaaga tgaacttaaa aatgagaatc ttgatgcttt gaaagtaggt tttgcaaatt
    24181 taaagcgcca tatacgtaat atggaacaat atcagctacc tgtgatcgtc gcaatcaatg
    24241 aattcgtaac agatacagac aatgaactca cactgttgga gcacttgtgt gaaaatcagg
    24301 ggattcttgc caagagagcg agtgtatggg cagatggagc agaaggcggc gttgatttag
    24361 caaaagctgt agtacgattg attgatagaa aagaagctaa ttacaagcca ttataccgcc
    24421 tagaagaaac gatacaagaa aagacagaaa caatcgtcaa gaaaatttac ggaggaaatg
    24481 gtgttgtttt cagcaaaaaa gcgcaaaaac aaatagaaga gtttacgaag aatggatggg
    24541 acaaactgcc gatttgtatg gcaaaaacac agtattcatt ttctgatgat ccttcactgc
    24601 taggtgcacc ggaagatttc accatcacga tccgcgaaat catcccaaaa ttaggggcag
    24661 gatttttagt agccttgact ggagatgtta tgacgatgcc tggtttgcct aaaaaacctg
    24721 ctgcattgaa tatggacgtg acaaatgacg gagaagtgtt gggattgttt taagcttgat
    24781 tataatctac catcgaagga gctgtatttt gactacagct tcttttttta gacgataggg
    24841 aattaatttt gtttacacta ggatcattct ttttctttat tgtattttga gctatactaa
    24901 agcataatag gagggtatga tgaaaacgac atttataaga aaaaccggtt tttacggtat
    24961 gggtagctcg attcagctaa gaatcaacaa tgaaaaacgc agtgttctca gtcatgaaca
    25021 aaagataacg cttgatctgg aagtgccctt tacgatgcag gtcacctttt attggctaaa
    25081 aagccccgtt tatacagtga aagaagcaaa gcctgcttat gtgatcacaa tgaattactt
    25141 gattcttcaa atctatccgt ttttatttct agcaactggg ttgggggccg ttttcctaca
    25201 aaatttattt ttcagttttt ttgcagttat tgtgttgatt gcgttctttt tctatataaa
    25261 aaaccgggca tatttgatca aggaggaaaa ttatggcaaa ctctgatctt tttttaatca
    25321 gtttcaatcg aatcgaaaaa tggatgagag acgagatggg aaatgcacga aatatgggat
    25381 ttaccgaact tgtcagacgc ctagctcaaa gaaaacaatt aatgatcaga aaatacgaag
    25441 atgatctttt acagcttgct cagttaagga atgcgatcgt ccatgatcgg atcgcagtcg
    25501 attttgtcat tgcagagcca aatgattgga cagtcaagcg aatccagctg atcgaacaag
    25561 aactgattca gcctgaaacc gtgctgcctc gatttgcaaa acgtgtaaca ggttttgaac
    25621 aagatcttcc gttacttgaa ctgttaaaga tagtagcaga aaaaaggtat tcacaatttc
    25681 cgttatacaa taaaggaaaa tttgtagcat tgattacttt acgtaatatc gggttttggc
    25741 tagcaaaaga aagccaaaaa ggtccggttg atttaacaaa taaacgagcg ttggatttga
    25801 tcatccaaaa cggaaagtac acaaattatc attttgttcc agctaaaaca catatttacg
    25861 aggttgaagc gatgtttcgt gagcaaggga ctttggaagc cgtacttatc actaaagatg
    25921 ggaatcccga tggtaatctg ctcgggatcg tacgacctcg tgatatttat aaacaaatag
    25981 aaaaggattg attttttgtt aattatttat tggatattca gcgctttgct agtaggagtg
    26041 gatcagttgg tcaaatggtg gatcactgat aatctagcgc ttggagagac gaaaaatctt
    26101 ataccaggta ttttgtcact gaatcatatt cgaaacactg gagccgcatg gagtatgcta
    26161 gaagggaaga tgtggttctt tactatagtg acactcattg cagtcgttgt gattctcaca
    26221 ttgatgatta aaaatcgtga aaaaggtaat cgctggttca tgattggatt gagtttgatt
    26281 ctagctggtg cgattggcaa ttttatcgac cgtattcgtc ttggctatgt agtagatatg
    26341 ttccagacag attttatgag ctttccgatt tttaacgtag cagatgtgac acttgtatgt
    26401 ggagtgattt gtatgttaat ttatattatt ttagatgaaa aggatcaaag aaaaaaatga
    26461 cagaacttaa aacgacgatt caccatgaat ctggtaggat cgataaagta ttgactaatt
    26521 tattcaccga ttacagtcgt tcgcaaattc agctgtggct gaaaaatgga gcggtttctg
    26581 tcaatggaca gccagtcaaa gcaaattaca aagtaaaaca aggagacgag attactgtct
    26641 tgatccccga accagaaaca ttagatgtgg tcccagaaaa tatcccgttg acgatcgttt
    26701 acgaagatga agatgtagcg gtgataaaca aacctcaagg aatggtcgtt catccttcag
    26761 caggtcatac gcatggcaca atggtcaatg cgctgatgta ccatatcaag gatctttcta
    26821 cgatcaacgg cgttattcgt ccaggtattg ttcaccgaat cgacaaagat acttccggac
    26881 tgctaatggt tgccaaaaat gaccaggcac acgaatcatt agctcagcaa ttaaaagata
    26941 agacctcttt gcgcaaatat gttgctttag tccatggaga gattcctcac gaaaaaggaa
    27001 ccatcaatgc gccaatcggc cgttcgaagg aaaaccggaa aatgcaggcg ataagagaag
    27061 atgggaagtc ggctgtcaca cattttactg tattggaacg tttcgaagga tttactctgg
    27121 ttgaattaaa actagaaacc ggaagaacac accagatccg tgtgcatatg aagtatatcg
    27181 gttatccgtt agctggagat ccagtatacg gaccgaagaa aactttgaaa ggcaatggtc
    27241 agtttctcca tgcgcaaatg ttaggtttta cgcacccgac tacaaacgaa aagatgatct
    27301 ttgaagcacc tcttccagaa gtttttgaaa aaactttaga aaaattaaga aaaaacattg
    27361 cattttaatt tagataacgg tatagtaatt aacaacgaaa gcataaaact taatagaatt
    27421 cctttaagtt tagtcccgtg aggctaagaa gggtgggagc aaggatacgt atggtgcgcc
    27481 ataagtagat tgctcattga catagtatag ctatcagtgt atcagtatac gctcagtcta
    27541 aacattcctc ctgtcctctt agggcaggag tttttttatg caaaaaagta gggggaatga
    27601 aaatgcaagc aaaagaagta gtagatcagg taaccatgaa gcgagcattg actcgaatta
    27661 cttatgagat tatcgagaga aaccatagta tccaagatat cgtattggta ggtatcaaaa
    27721 cacgaggaat ctacattgca tcaagaattg ccgaacgcct gaagcagcta gaagatatcg
    27781 atattcctgt aggagaactg gatatcactt tataccgaga tgacaaaaaa gaaaatccag
    27841 aagaaccgga acttcattct tctgacatcc cagtttcctt agaaggaaaa gaagttattt
    27901 tgattgatga tgttttgtat acaggacgaa cgattcgagc agcaatggac gcagtaatgg
    27961 attttggcag accaagaaaa atttcattag cagtcctggt cgatcgtggg catcgagaat
    28021 tgccaatccg tgcagattat gttgggaaga acattccaac atctcgggcg gaagagattt
    28081 tagtagagat gcaagagctt gatggacaag atcgcatcat gattttgaaa gaggaagatt
    28141 agaggagaga gaaacatgtc ggagaaaaaa ggatttcata atgatgacgt ggtgttagat
    28201 atccacgatc gcccaacccc aggacattgg gtaggattaa gtttacaaca cttgttcacg
    28261 atgttcggag caaccgtgtt agtccccatc ttagtaggta tcgatccagg tattgcgcta
    28321 gtcagttcag gactaggtac attggtgtac ttatttacta ctaaaggaaa gatacctgcg
    28381 tacttaggaa gcagcttcgc tttcattgca gcgatgcagc ttttgatgaa aacagatggt
    28441 tatccagcga ttgcacaagg agcagtcaca actggattag tctatttgat tgttgcgctg
    28501 atcatcaaaa agatcggttc agcatggctg gataaaatct tgcctccaat cgtcgtcggt
    28561 ccagtcatca tggttatcgg gttgggactt gcggcaaatg cagcaacaaa tgctatgtat
    28621 aatcagggca cgtatgattt caagtatatt gctgtagcgc tgatcacatt aggattaaca
    28681 attttcttca acatgtggct aagaggtttt ctcggattga ttccgatttt actaggtatt
    28741 attagcggat acatcgtagc cttgctgttt ggaatcgtgg atactaagcc aatcgtagac
    28801 gctgcctggt ttgctgttcc taactttgag attccctttg tacaatatga accaaagcta
    28861 tatcttggag caatcacaac aatggcaccc atcgcttttg tgacgatgac agagcatatc
    28921 ggccatctta tggtattgaa taaactaact aaacgtaatt tctttgaaga accgggtctt
    28981 cataagacgt tgacaggtga tggattagca caaatcgtgg cagcctttgt cggaggtcct
    29041 cctgtaacaa gttacggtga aaacattggt gttctggcta tcacacgtgt ccatagcgtg
    29101 tttgtgatcg gtggagctgc agtatttgca gtcattttag gattcgtagg aaaattaagc
    29161 gctttgattt taagtatccc tggaccagtc atctcaggaa tcagttttat cttatttgga
    29221 gtaattgccg caagtggaat gaagatttta atcgagaaca aaatcgattt tgataaaaag
    29281 aaaaatctgt tgattgcttc agttatctta gtagtaggaa ttggcggact cgtctttgaa
    29341 gtaggcacat ttacattgtc ggcaatggca ttagcaactg tacttggcat cattttaaat
    29401 cttatcttac cagaaacttc acgaagcgaa gaaaactaag gaggaaacat tatgatcatc
    29461 aaatcagaac gtatcagctt aaagcattta ttgaccgttg aggcacttac tgatcaagaa
    29521 gttatgggct tgatccgcag agggcaggaa ttcaagcaag gtgctaattg gactccgcaa
    29581 aagcgccagt actttgctac aaacctcttt ttcgaaaata gtactcgcac acataaaagc
    29641 ttcgatgtag cggagaaaaa actagggcta gaagtgattg aatttgaagc aagcacaagc
    29701 tctgtccaaa aaggggaaac actatacgat acagtactga cgatgtcagc attaggcgta
    29761 gacgtagcgg ttatccgtca tggtgatgag aactattatg acgaactgat acaaagtaaa
    29821 acaatccagt gttcgattat caatggagga gacggaagcg gacagcatcc aacccaatgc
    29881 ttgcttgatt tgatgaccat ttatgaagaa tttggtcatt ttgaaggtct gaaagttgcc
    29941 atcgtaggtg atatcactca ctctcgcgta gctaaatcca atatgcagat gttaaaacgt
    30001 ctaggcgcac aagtattctt ctcaggtcct agagaatggt acgacgatga attcgaagtg
    30061 tacggacatt atatgccatt ggatgaacta ctagatcaag ttgatgtcat gatgatgctt
    30121 cgggtgcaac acgaacgcca tgatggaaaa gaaagtttct caaaagaagg ctaccatcag
    30181 gaacatgggt taacagttga gcgtgcgaag aagttgcaaa aacatgcaat cattatgcac
    30241 ccagcaccag ttaaccgtga tgtagaatta gctgattcat tagtggaagg attacagtcg
    30301 cggatcgttc agcaaatgag caatggagta ttcgtcagaa tggcaatctt agaagcagtg
    30361 ctaaatggaa aagcgtaatt ataggaggat gaaaaaatga aaacactcat taaaaacggt
    30421 cagatcaata cacgaaaaaa tatgacgaca ccagcagaaa tttggataga agatgggcga
    30481 atcaaagcaa tcggtacagg attctcagaa gccgaatttg atgaagtgtt tgatgcaaag
    30541 ggacaattga tcacacctgg tttagtggac gtccatgttc atttgagaga gccaggattt
    30601 acatacaagg agacgatcga agctgggact cgctcagctg ctcgtggcgg tttcactact
    30661 gtatgcgcga tgccgaattt aaatcctgta cctgatacgg cagaaaagct gagacaagta
    30721 tatgacatca ttagaaaaga tgcagttgtg aaggtattgc agtatgcgcc aatcacagaa
    30781 aatctgcgaa gtgagaaatt agttgatcaa gaagcactga ttgaagaagg tgcttttgct
    30841 ttcacaaatg acggtgtagg tgtccaaaca gctgggacga tgtatctggc tatgaaagaa
    30901 gctgcaaaaa acaataaagc cctagtagcc catacagaag atgaatcttt attatttggc
    30961 ggcgttatgc acgctggcaa gaaggcagaa gaacttggtc ttcccgggat cttaagcgta
    31021 accgagtctt ctcaaattgc tagagatctg ttgctagctg aagcaactgg tgttcattac
    31081 catgtttgcc atgtttctac taaagaaagt gtccgtgtaa tcagagacgc aaaaaaagca
    31141 ggaatccacg taacagcaga agtttcaccc catcatctta ttttgatcga cgaagatatt
    31201 cccgaagatt tcggcttttg gaaaatgaac ccgccgctaa gaggacggga ggacagagaa
    31261 gctttgatcg aaggcttgct tgacggtacg atcgattgca tcgctacaga ccatgccccc
    31321 catggattag aagaaaaaag ccaaagtttc atgaaatcac catttggtat cgtaggaagc
    31381 gaaacagcgt ttcaactgat ctatactcat tttgtagaaa ctggtcgatt cacgttggaa
    31441 caagtgatca attggttggc agttaaaccg gctgagattt ttggtttgaa tgcaggaacg
    31501 ctaacggttg gtgcccccgc tgatgtagca gtttttgata ttacacaaac ttgcacgatc
    31561 gataaagaag actttttatc aaaaggggag aatacacctt ttatcggctg gaaagtaaaa
    31621 ggcgaaacac aaatgacttt tgtcaacgga aaacttgtgt ggcagaaggg agaataaaag
    31681 tatggaacgt ttgcttatat tagaagatgg aacagttttc gaaggaaagg cttttggtgc
    31741 agatgctagt gtcttcggag aaatcgtttt tacaacaagt atgactggtt atcaagaaac
    31801 tatcactgat caaagtttta acggtcaaat cattacattt acctatccta tggtgggtaa
    31861 ttatggggta aatagagatg attatgaatc gatttctcca acatgcaaag gagtagtagt
    31921 aaaagaacat gcacgtgtag cttcaaactg gcgaaaccaa atgacgctag attcattttt
    31981 gaaacaaaaa ggaattcctg gaatatcagg tatcgatacg cgcgccctta ctcgcaaaat
    32041 ccgtgaagtc ggaacgatga aagctagtat agttgatgct ggagattcct tcgaacatgc
    32101 atttgatcag ctgaaagcaa ccgttctagc aaccaatcaa gtgcagcaag tctcaactaa
    32161 caaaccttat ccaagtccag gaacaggtcg gaaagtcatt gtcattgact tcggcttgaa
    32221 acatagtatt ttacgagaat tgagtaaaag aaactgtaac ctcacagtac ttccctataa
    32281 cacagatgcc gagacgattt tagcgctatc accagacgga gtcatgctga cgaatggacc
    32341 tggtgatcca aaagatgtgc cagaagcaat cgaaatgatc aaaaaaatcc aaggaaaagt
    32401 accgattttt ggtatttgtc tcggtcacca gttatttgct ttagccaacg gtgcagatac
    32461 ctataaaatg aaattcggac atcgaggatt aaatcacccg gtacgagaag tagcgaccgg
    32521 aagaattgat tttacttctc aaaatcatgg ctatgcagtc gatgaagcca cgattgatcc
    32581 agaaaaactc atcgtaactc atgtagaagt aaatgacgga acagtagaag gagtcaaaca
    32641 cagagattat cccgctttca gtgtgcagtt tcatccagat gctgcccctg gtccgcatga
    32701 tgcactacat ttatttgatg aatttatgga aatgatggac gcaggaaagg agcagtaaac
    32761 agatgccgaa acgtacagat atcaagaaaa ttatggtgat tggatctggt ccgatcatca
    32821 ttggtcaagc cgcagaattt gactacgcag gaacacaagc ctgccttgcc ttaaaagaag
    32881 aaggatatga agtggtttta gtaaattcta atcctgcgac gatcatgacg gataaagaag
    32941 tggcagatca agtatatatc gaaccgatca cacttgaatt tgtatcaagg attcttcgaa
    33001 aagagcgtcc agatgctctc cttccaactt taggcggaca gacaggcctc aatatggcaa
    33061 tggagctggc agcatcaggt attttagaag aattgaacat agaacttcta ggaacaaagc
    33121 tttctgcgat cgatcaagca gaagatcgtg atctattcaa acagttgatg aaagaattga
    33181 atcagccaat ccccgagtct gaaatcgtca atacagtaga agaagctgta gattttgcaa
    33241 ataaaatcgg ctatcctgtc atcgttcgtc cagcctttac tttaggcgga actggtggag
    33301 gtatgtgtga aaatgaagaa gaattacgta tcgtatcaga aaatggatta agtctttccc
    33361 ccgctaccca atgtttgatt gaacgaagta ttgcaggttt taaagaaatt gaatatgaag
    33421 tgatgagaga ttcggctgat aatgcaatcg ttgtctgcaa tatggaaaat tttgacccgg
    33481 tgggtatcca tacaggtgat tctatcgtat ttgcccctag tcaaacattg tctgaccatg
    33541 aatatcaaat gcttcgtgat gcttctcttt cgattattcg agcacttaag atcgagggag
    33601 gctgtaatgt ccaactcgct cttgatccac atagttttaa ttattatgtg attgaagtca
    33661 atccgcgtgt ttctcgttca tctgcgttag caagtaaggc gactggctat ccgatcgcta
    33721 agctagctgc taaaatagcg cttggattaa cattagacga aatgaaaaat cctgttacag
    33781 gaacgacttt tgcagaattt gaacctgcat tagattatgt tgtagctaaa attcctcgct
    33841 ggccgtttga taagtttgaa aacggagaac ggcttttagg cacgcagatg aaagcaactg
    33901 gtgaagtgat ggctatcggt cggaatatcg aagagtcttt attgaaagct gtgcgttctt
    33961 tagaaatcgg tacacaccat atcgaacttc ctgagttggc agacatcacg gacgatgacc
    34021 taatccaaaa agtggttcga gcacaagatg atcgcctgtt ttatgtagct gaagctatta
    34081 gaagagggta caccatagaa gaattagcgg atttgacaaa aatcgattta ttctttttag
    34141 ataaactgtt gcatatcgta gaaatcgaac agcagttgaa acagcagccg ttggatgaca
    34201 agatattgaa aacagcgaaa caaaacggtt ttacagacaa aaaagtggcg gagctgtggg
    34261 aaataaacga agaagccgtt cgccagatga gaaaagaaaa tgatttgatt cctgtatata
    34321 agatggttga cacttgtgca gcggaattcg aatcaaaaac accttatttt tatagttcct
    34381 atgaatatga aaatgaaagt tcacgatcag aaaagccctc aattctagta ctgggatctg
    34441 gtcctatccg tatcggacaa ggggttgaat ttgactatgc gactgtgcat tctgtcaaag
    34501 caatccagca ggcaggatac gaagcaatca tcatgaacag caatcctgaa acagtttcga
    34561 ctgacttttc tatttcggat aaattatatt ttgagccatt gaccttcgaa gatgtgatga
    34621 atgtgatcga gctagaacag ccgataggag taatcgttca atttggtggt cagacagcaa
    34681 tcaacttagc cgaacctttg actgctgctg gtgtaaaaat cttagggaca acgatcgaag
    34741 acttggatcg tgcggaaaat cgtgatttat tcgagcaagc gctgaaagag ttggatattc
    34801 ctcaaccttt aggggatact gctacaagta aagaagaagc cgtggttatc gcttcgagaa
    34861 ttggttatcc agttcttgtt cgtccaagct atgtattagg cggacgtgcg atggaaatcg
    34921 ttgaaaacca gcgagattta gaagattaca tggaacatgc tgtgaaagca tctccagaac
    34981 atccagtact agtggaccgt tacttgcttg gtggtgaatg cgaagtggat gcgatttgtg
    35041 acggtgaaac agtactcatc ccagggatca tggagcatat tgagcgtgct ggtgtccatt
    35101 caggggattc aatggctgtg tatccacctc aaacattatc tgaatcgatc aagaaaacga
    35161 tcgaagatta cacgatccgc ttagcattag gtttgaattg tgtcgggatg atgaatatcc
    35221 agtttgtcat ccatgaaaat gaggtttatg tgatcgaagt caatcctcga gcaagtcgga
    35281 ctgttccatt cttaagtaag gtgacaggaa ttccaatggc acaaattgcg acgaaagcta
    35341 ttttgggaga gaaactaaag acacttggtt atgaaaacgg actttatcca gaaacaaaag
    35401 aagttcatgt taaagcacct gtcttttctt ttacgaaatt gcagaaagtt gatacttact
    35461 tagggcctga aatgaaatcg actggagaag taatgggatc tgacagacaa ttagacaaag
    35521 ctttgtacaa ggcattcgaa gcttctggat tacacttgcc tgaatatgga gccgtattat
    35581 ttacgattgc ggacgaaaca aaagaagaag cactagaatt agctaaacgt tttacagaaa
    35641 ttggctatag tctgatagga acgaaacata cagctgctta tttcgaaaaa aatggtctgg
    35701 tcgttgaaac agtagcgaaa atttctgagg aagcagcaga aaaaaatgta gtcgacttaa
    35761 taagagatgg taaaacacaa gtggttgtca atacgattga taaagatcgg ggaaatgctt
    35821 ctaaagacgg tttcatcatc cgaagagaag cagttgaaca tggtattcca ttgtttactt
    35881 ctttagacac agcagatgcg atcgttcgag tgatggaatc ccgtgctttt tcaactaacc
    35941 ctatttaacg gcgagcacat gttaagacaa aaagacgaaa aaccgttgca ggaggggaca
    36001 gatgaaacaa gaaatgatgg aaatcgttca gcaaaaacag ctggcaccaa agatttttga
    36061 aatgacattg aaaggtgatc tagtaaaaga aatgcagcaa ccaggacagt tcttgcatat
    36121 tctcgtgccg cgtgcagact tgctattacg ccgaccgatt agcttgaatc atatcgacca
    36181 cgaaacaaat acttgccgta tcatttatcg ggtggaaggt gaggggacta gagtcttctc
    36241 tactctttct gccggagacc atttagatgt catgggtcct ttaggcaatg gttttgattt
    36301 gtccaatctt catacaggag acgaagtatt tataatcggt ggaggaatcg gtgtccctcc
    36361 tctatatgag ttatcaaaac agttagtcga aaaaggaatt aagccaacgc attttttagg
    36421 ttttgccaca catgaagtcg tttattatga aaaagaattt aatcgtctag gagaaacaag
    36481 aatcgctact gacgatggaa catacggtat gcacggaaat gtcggtaatt tattgttagg
    36541 aacgaagacg gaacctacag ctgtttttgc atgtggttca aacgggttat tgaagacagt
    36601 ggaacagctg ttttcttcgc atcaaaatgt ccagttatca ttagaatccc gcatggcatg
    36661 tgggataggg gcttgctatg cttgcgtttg tcatacgccg gaagatgaaa caggaacaaa
    36721 aagtgtgaaa gtttgtgacg aaggaccgat ttttaaaata ggagaggtaa ttatatgaat
    36781 gagaatcctt tagcggtcaa acttcctggc ctagaattaa aaaatcctat catgccggca
    36841 agcggctgtt tcggttttgg caaagaatac ggaaaatact atgatttgaa tcagcttgga
    36901 tcgatcatgg tcaaggcaac gactccaaat gcccgctttg gcaatcccac gccaagagtg
    36961 gcggaaacac catctggcat gttgaatgca attggtttgc aaaatcctgg tctagacgtt
    37021 gttatgcata aacttcttcc agaattagag gagtacaatg agttacccat catcgcaaat
    37081 gttgctggtg cttgtgaaga agactatgta gaagtttgta gcaaaattgg cgaagcacca
    37141 aatgtgaaag caatcgagct gaacatttct tgcccaaatg tcaaacatgg cggaattgct
    37201 tttggaacag attctgatgt tgcttttcaa ttaacacaag cagtaaagaa agtttcttct
    37261 gttcctgtct atgtcaaatt gtccccaaat gtaacagaca tcgttccaat cgctcaggca
    37321 atcgaagctg gaggggcaga tgggtttacg atgatcaata ctcttcttgg tatgcggatt
    37381 gaccttaaga caagaaaacc gattttagcc aatcaaacag gtggactctc cggaccagca
    37441 atcaagcctg tcgctattcg tttgattcac caagtagcaa gtatatccag ccttccaatc
    37501 attgggatgg gcggcgtgca gacagtggat gatgttcttg agatgtttat ggctggagca
    37561 agtgctgtag ctatagggac cgcgaatttc accgatccgt atatctgtcc taaactaatt
    37621 aaagagctcc ctgtgcgaat gagcgaacta ggcatcgaat cgctggaaag attaatcaaa
    37681 gaagtgagag aggaacgtga acgatgaatc aactagacca acgaccaatt attgcattag
    37741 atttttcaac taggcaagag gtagaagact ttcttcgatt tttcccgaaa gaagaaaaat
    37801 tgtttgtgaa aatcggaatg gaattgttct accaagaagg acctgaaatc gtacgttatc
    37861 taaaagaggc tggtcataat gtatttttag atctgaaact tcacgatatt ccgaatacgg
    37921 tcgaaaaagc aatgcgggga ttggcaaagc ttggtgttga tctcacctgt gttcatgccg
    37981 ctggcggtat tcgtatgatg gaggcggcaa tgcgaggatt agaagagggg actccagaag
    38041 gtggaaaacg tccgctactt ctagctatca ctcagcttac ttctacaagt gaagaagaaa
    38101 tgcatgccga tcaactaatc gaagtttctt tagaaaaaag tgtgatccat tatgctagct
    38161 gtgccaaaaa agcaggatta gatggtgtgg tttcttctgc ttgggaagta gaagcgatca
    38221 aagaaatggc aggagacgaa tttgtttgct taactccggg tataagacca gaaggaacag
    38281 tcgcaggaga tcagacaaga gtagtcacac cttctcaggc aaaaaagatc ggctcaactt
    38341 ttatcgttat aggtcgacca atcactcagt caactaatcc ttataaagca tatcaaacga
    38401 ttcaaacaga atggagctaa cctaaaatga atgttgaaca aagtatcgca aaagacttat
    38461 tagaaattga agcagtattt ttaaatccaa gtaatccttt tacatgggca agtggaatca
    38521 aaagcccaat ttactgtgat aaccgaatca cgatgagcta tccaaaagtc cgaaaagaga
    38581 tcgcaaaagg attagcaagc aaaatcaaag aagcttaccc tgaagtacaa gtgattgctg
    38641 gaactgcaac agcaggaatc cctcatgccg catgggtagc ggaaatactg gatttgccaa
    38701 tggtctatat tcgaagtaag gcgaaagatc atgggaaagg taaccaaatc gaaggacgta
    38761 tatttgaagg tcagaagatg gttgtgatcg aggatttgat ttctacaggc ggaagtgtct
    38821 tggaagcagc tgaagcagca aaaagagaag gtgcggatat ccttggtgtt gcggctatct
    38881 ttacgtatga gttgccaaaa ggaaaagcaa atttcgaaaa agccgaaatc cctcttatga
    38941 cgttgaccaa ttattctgta ttgattgaag cagctttaga agatcgttat atcgatgagc
    39001 aagaattaac tctgctaaaa gaatggaaaa aagatcctga aaattggcag atttaagcgc
    39061 gcttgatgaa gatagagaaa tccgatttgt gtctccagaa tcatcaattg gaatagcaaa
    39121 aatccaaaaa ctgattgcga gaagcatctt ttcatcaggt atgaaaagaa acttctatcg
    39181 tgatcagttt ttgatttttt taaaattcat ttttctaaga ctggttagca gaaagaagag
    39241 aataatctct tctctttttt ctattatata tagattttct ctattttttg gtaagggagc
    39301 tgtttcaaaa atggtaaaga gcggaaatgt tgcgaatatc agttatttaa tgatatgata
    39361 ttcggaaaaa gaaatctcaa agggaggtaa cttttatggg gttgaatgaa tttattttag
    39421 gattgaacaa tttagagacg atcacaaggg cacctggatt ttttaaatat acagagcata
    39481 cagttgcagc acattcgtat cgtgtagcat ctattgcgca agttctagga gacattgaag
    39541 aagcgaatgg acaaccgatc gattggaaac tgctctatga aaaatcattg aatcatgact
    39601 acacagagcg ttttattggt gatatcaaaa cgccggtgaa atacgcaagc aaagagttaa
    39661 gagggatgct gcaaacagtt gaagaaaaga tgacggacga attcatcaac caagagattc
    39721 cagctgagtt ccaagaagtt tatcgccgtc ggttgttcga aggaaaagat gaaaccgtag
    39781 aaggagaact attagccatt tcagataagg ttgaccttct ttatgaatct tttgatgaaa
    39841 tcatcaaaaa taacccggaa aaagtttata aggagatgtt cattgaggca gtgactacga
    39901 ttaaaagctt tgaacatcgg ccttctgttc agtatttctt taaagagata tttccggaac
    39961 tattgaacca ggaattttat ggaaaagagg cgtttttaga aaccgtctca acgattgtaa
    40021 agaaagaaga aaaataaaaa tgatatgaga tgcaggggga agtatagttg aaaaagaata
    40081 tggatgtcga atggggctat tctggagaca aaggtccgga aaattgggcg agtttatgtg
    40141 attggtttgc gagaggtgcg gaatttccat tgcagtcgcc tattcatttg accaaggggg
    40201 aagaaaccaa aatagaagga gacacgcttt cctttcatta tcaaaagcaa cggtttactg
    40261 acaaggaatt caaaaacacg attcatttag taccttttga ccaactcagt tatgtagagt
    40321 tcaagaaaga acgctattat ttgacagata ttcatttcca cttgccttct gaacatatca
    40381 tcaacggaaa acaggaagat attgaatttc attttgttca tatgaacagc aaaaaagaga
    40441 atctagttgt gggtgtcatg taccagctaa tggtacagga aggttggctg tataatcaag
    40501 aaaatgggga aagttggaac ttagaaaaac atgagcattg ggtaaatccg gatgtgttct
    40561 ttccggagca aaaaagccat ttccattata ttggctcatt gacaactccg cctaccagcg
    40621 ggccaatcca atggtttgta atggatcatg taggaaaact gaaccaagag tttcttctcc
    40681 ccttcgatgg tcagtatgct cgaccgaaca accgaccaat ccagcccttg aatggaagag
    40741 aaatttatta tttcgaggag tttgagcaat gaatattcag cagatgaaat acgtggtcgc
    40801 tgtagcaaac aatggtagtt tcagagaagc ggcaaagaaa atgtttgtct cccagccaag
    40861 tctttcagct ggtatcaaag aattagaaac cgagttaggg atcacgctgt ttactcgaac
    40921 gaatcgaggg gcatatttaa cagaagaagg gcaagacttc ttaaatcgtg ccgagaaaat
    40981 attggtccag cttgatacat tagaaactca ctatctaacg agtgaaaaga gagacaattt
    41041 ttcgatcgct gcccagcatt atgatttctt aggtcctttg accgcacagc tactcgagga
    41101 attcaaagac gagatcaaac aatttcggat catggagacg acaacagcga gagtgatcga
    41161 agaagtcaaa gagcagcaca gtgagatcgg cattatttat atgaatgagc ataatcaagc
    41221 tggtatcaga agatatttac aacaaggaga gctaagtggc cgcagcattg gaaaattcaa
    41281 aacgcatatt tttttaaggc aaggacatcc attagctgaa cacgcagaga tcaccaaaga
    41341 agaattgtta tcttatcctc aagttcgttt tacacaggat ggaaataatt tcccgtattt
    41401 ttatgaagac cttatcgaga tccctgatca agaaacagtg atttatacga gtgatcgagg
    41461 tactttgatg aatatcgttc ttgaaactga tgcttacgct tcaggatctg gtattgtgat
    41521 tggagggatt cgcgagcact tgcggttaat ccctttggca gaaggcgaac taaatgaatt
    41581 ttatatcatt tattcggcga aacgtccgct cagtgaaatc gctcaaagat ttatcaaaga
    41641 gcttactcgg ttactggacc agcagagcta agaaaagcag atgagaacgc tgtttattga
    41701 acaccgttga tcaaaaaaga ggggttgtga cagaattttg tcacaactcc tctttttgta
    41761 gcaaacaatt tataactaat tgctgttgtg ggaaagtacg caaatatgat tctagaaaca
    41821 ctcattgatt acctgttttg tttcatagct gtaatttttt cttcttctgg tgtcacgata
    41881 atcgtctttt ggttttcata aataacataa ccaggcttgg cgccatttgg ttttcggata
    41941 tgctttacct gtacaagatc aaccggcact tgggcagagt atcggtattt tgaaaaatag
    42001 gcagctaact cggcagcttc tgtaattgtt tcatctgagg gcttgtcact tttaatgatg
    42061 acgtgcgaac cgggtatatt ttttgcatga agccaatagt cggttttttt agctgtcttc
    42121 attgtcagct ggtcattttg aagattgttt ttaccaacaa gaatgagcgt tccgtcagaa
    42181 gaaaagtatt gatcaggctg agatggtttc ttacgttttt gtttttttaa gttcttcttt
    42241 ttcaggtatc cttctgaagt caattcttct ttaattgctt cgatatccat tgggccggca
    42301 atttccagtt gagataagac agattcaaga tattggattt catctttggc ttcttggatt
    42361 tgttcgccaa tcagcttaac agcgttcttc agcttttggt atcgatggaa atatttctgt
    42421 gcattttgat ttggtgtcaa tgccggatct agtgctattt tgattggccg atcttcttca
    42481 taataattag gtaaaacaac ttcttttgcg ccgcgtggta cttgtgtcag aaaagtagta
    42541 agtagttcgc catttctacg gtaattctca gcgttttcgg attctttgag tgtttgttca
    42601 cgttttttca atttattttt atttcgtttt agttcatttt cgatttttcg aatcagttct
    42661 ccgccttgtt gttttgcccg atctttctct gctttttcgt gataatagct atctaataat
    42721 tgactgagtg ttggataagc tgttacagca gatgcctgtt cggacaatac ctgataagca
    42781 atcggtgtga aaaaatcttt attttccgtt tcgtaaaaag taggaatagg ctgatggctg
    42841 atggctgaga aaaattcgtc ccatacaacc atcttttcat taggacgttc cagtaatctt
    42901 ttggtcagct cttgagcgat atcgaaaccg attccctgaa actgacgttg gattgctttt
    42961 ggatctagtt cagtttggga aagccgctga aagatttttt ctttctcgga gctaaatggg
    43021 ttcaagacct cttgttttgg cggagcaaca tattctacac caggaagcaa agaacgataa
    43081 gtgttttgag agcttccaat gtgtttgatc gcatccagta tttttccagt ttctcgatta
    43141 actaagacga tcgtactatg acgtcccatt aattcaacga tcaatatgat attttgtaga
    43201 tcacccaact cgtttcgttt agcaaagtgg aaatggatca ctcgatcatt ttcgatttgt
    43261 tcgatgtctt ctaaaatagc gccatccaaa tattttcgaa gcatcatcac gaagttaggc
    43321 ggattatcag gattctgata atcgatttgg gtaatctgta cacgagcata acttgggtgc
    43381 gcagataata ataatcgatg gtttttgccg cgtgaacgaa tcaccagcac gatttcattt
    43441 tcataaggct gatggatttt cgagatccgg ccagatagta acgtttcacg caattcgttg
    43501 accatcgcgt gggtaaatac accatcaaag gacatggttc attcctcatt tcttttcata
    43561 agtatattca ttataacgaa aaaaagaaga aaaaccaatt tggccgaata gtaaagcaaa
    43621 agtctagaaa aatcttggac taattacata aaaaacagtg tttctatcgt tgaaatccaa
    43681 atttttaggt ataataaaaa ataggagaaa aagtagaaaa gaaggtgttt gttatttggg
    43741 aagtagttaa atcgagagta caagaaatga atgaacattt ctcagtgtca ttcgcagaaa
    43801 atccaagtaa gtattttcga cgtcttgttt ttctgctcat cttatcattg ctgttaacag
    43861 ttgcaatgtt tttattgccg gaaaaaatcc gagaagagca agtaaatagt ctcgtttatt
    43921 cgtccatacc aaccagcaaa ttgaaacctt ttgattacag caaagctgat cagaaaatca
    43981 aacaaagccg ggcgatatcg gtgatgttca gcaaaccgaa cgggaaaacc tatcaggaca
    44041 tgcttgatgt ttttaatgat cctgagctta tggaagaatt gaatcgaccg atcttttatt
    44101 atccgatcgt ttatgatgta catgaactgg aacaaaaata taacatacga acggatgaag
    44161 tgacatttat tttctttgat ggaggaaaag aagcaaatcg gataactgcg ggtaagggag
    44221 gaataactga ttttgataag gagttgatcc ctgaactgaa tcgacttccg ttagccaata
    44281 tcaaacaatt agaagaagaa ttggatagaa cgggagtaac ttcccaaaat tccgagattg
    44341 ctaattgaga cattggcgcg atgaaacggt gggtctatat agc
//
LOCUS       DAHHZJ010000008        38360 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.008, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000008 DAHHZJ010000000
VERSION     DAHHZJ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.008"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            146..793
                     /locus_tag="NBL02_000317"
     CDS             146..793
                     /locus_tag="NBL02_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HCD2571032.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            808..2334
                     /locus_tag="NBL02_000318"
     CDS             808..2334
                     /locus_tag="NBL02_000318"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCD2571033.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(2383..5181)
                     /gene="ileS"
                     /locus_tag="NBL02_000319"
     CDS             complement(2383..5181)
                     /gene="ileS"
                     /locus_tag="NBL02_000319"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCD2571034.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(5443..6150)
                     /locus_tag="NBL02_000320"
     CDS             complement(5443..6150)
                     /locus_tag="NBL02_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HCD2571035.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            complement(6171..6953)
                     /locus_tag="NBL02_000321"
     CDS             complement(6171..6953)
                     /locus_tag="NBL02_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HCD2571036.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            complement(7002..7289)
                     /locus_tag="NBL02_000322"
     CDS             complement(7002..7289)
                     /locus_tag="NBL02_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HCD2571037.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            complement(7304..7903)
                     /locus_tag="NBL02_000323"
     CDS             complement(7304..7903)
                     /locus_tag="NBL02_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HCD2571038.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            complement(7917..8594)
                     /locus_tag="NBL02_000324"
     CDS             complement(7917..8594)
                     /locus_tag="NBL02_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HCD2571039.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            complement(8611..9852)
                     /gene="ftsZ"
                     /locus_tag="NBL02_000325"
     CDS             complement(8611..9852)
                     /gene="ftsZ"
                     /locus_tag="NBL02_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCD2571040.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            complement(9875..11200)
                     /gene="ftsA"
                     /locus_tag="NBL02_000326"
     CDS             complement(9875..11200)
                     /gene="ftsA"
                     /locus_tag="NBL02_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCD2571041.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            complement(11356..12576)
                     /locus_tag="NBL02_000327"
     CDS             complement(11356..12576)
                     /locus_tag="NBL02_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HCD2571042.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            complement(12591..13679)
                     /gene="murG"
                     /locus_tag="NBL02_000328"
     CDS             complement(12591..13679)
                     /gene="murG"
                     /locus_tag="NBL02_000328"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCD2571043.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            complement(13705..15066)
                     /locus_tag="NBL02_000329"
     CDS             complement(13705..15066)
                     /locus_tag="NBL02_000329"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCD2571044.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVSECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            complement(15080..16042)
                     /locus_tag="NBL02_000330"
     CDS             complement(15080..16042)
                     /locus_tag="NBL02_000330"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCD2571045.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            complement(16068..18254)
                     /locus_tag="NBL02_000331"
     CDS             complement(16068..18254)
                     /locus_tag="NBL02_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCD2571046.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPSED
                     PDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYRNL
                     EADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVTGW
                     SKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            complement(18255..18656)
                     /gene="ftsL"
                     /locus_tag="NBL02_000332"
     CDS             complement(18255..18656)
                     /gene="ftsL"
                     /locus_tag="NBL02_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCD2571047.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            complement(18661..19620)
                     /gene="rsmH"
                     /locus_tag="NBL02_000333"
     CDS             complement(18661..19620)
                     /gene="rsmH"
                     /locus_tag="NBL02_000333"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCD2571048.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            complement(19641..20072)
                     /gene="mraZ"
                     /locus_tag="NBL02_000334"
     CDS             complement(19641..20072)
                     /gene="mraZ"
                     /locus_tag="NBL02_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCD2571049.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            complement(20242..20610)
                     /locus_tag="NBL02_000335"
     CDS             complement(20242..20610)
                     /locus_tag="NBL02_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HCD2571050.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            20781..21737
                     /locus_tag="NBL02_000336"
     CDS             20781..21737
                     /locus_tag="NBL02_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCD2571051.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            21810..21926
                     /locus_tag="NBL02_000337"
     CDS             21810..21926
                     /locus_tag="NBL02_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HCD2571052.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(21981..23663)
                     /gene="recN"
                     /locus_tag="NBL02_000338"
     CDS             complement(21981..23663)
                     /gene="recN"
                     /locus_tag="NBL02_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCD2571053.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(23681..24130)
                     /locus_tag="NBL02_000339"
     CDS             complement(23681..24130)
                     /locus_tag="NBL02_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HCD2571054.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(24237..25055)
                     /locus_tag="NBL02_000340"
     CDS             complement(24237..25055)
                     /locus_tag="NBL02_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HCD2571055.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(25064..25954)
                     /locus_tag="NBL02_000341"
     CDS             complement(25064..25954)
                     /locus_tag="NBL02_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCD2571056.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(25956..26180)
                     /locus_tag="NBL02_000342"
     CDS             complement(25956..26180)
                     /locus_tag="NBL02_000342"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCD2571057.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(26185..27522)
                     /locus_tag="NBL02_000343"
     CDS             complement(26185..27522)
                     /locus_tag="NBL02_000343"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCD2571058.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(27523..28377)
                     /locus_tag="NBL02_000344"
     CDS             complement(27523..28377)
                     /locus_tag="NBL02_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HCD2571059.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(28474..28923)
                     /gene="nusB"
                     /locus_tag="NBL02_000345"
     CDS             complement(28474..28923)
                     /gene="nusB"
                     /locus_tag="NBL02_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCD2571060.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(28916..29341)
                     /locus_tag="NBL02_000346"
     CDS             complement(28916..29341)
                     /locus_tag="NBL02_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCD2571061.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(29587..30651)
                     /locus_tag="NBL02_000347"
     CDS             complement(29587..30651)
                     /locus_tag="NBL02_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCD2571062.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(30823..31068)
                     /locus_tag="NBL02_000348"
     CDS             complement(30823..31068)
                     /locus_tag="NBL02_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCD2571063.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(31207..31581)
                     /locus_tag="NBL02_000349"
     CDS             complement(31207..31581)
                     /locus_tag="NBL02_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HCD2571064.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            complement(31664..32578)
                     /locus_tag="NBL02_000350"
     CDS             complement(31664..32578)
                     /locus_tag="NBL02_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCD2571065.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            complement(32599..33402)
                     /locus_tag="NBL02_000351"
     CDS             complement(32599..33402)
                     /locus_tag="NBL02_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCD2571066.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            complement(33435..34424)
                     /gene="manX"
                     /locus_tag="NBL02_000352"
     CDS             complement(33435..34424)
                     /gene="manX"
                     /locus_tag="NBL02_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCD2571067.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            complement(34527..35018)
                     /locus_tag="NBL02_000353"
     CDS             complement(34527..35018)
                     /locus_tag="NBL02_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCD2571068.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            complement(35210..38017)
                     /locus_tag="NBL02_000354"
     CDS             complement(35210..38017)
                     /locus_tag="NBL02_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCD2571069.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
BASE COUNT        10718 a         8334 c         6498 g        12810 t
ORIGIN      
        1 agaatgagtc ttctctatca tctttcgttt tttgttgtaa aaacgtctaa aaatcctttg
       61 ttttttattt agaaaaatag tcaattaacg ttttttttgt tacacttact aatgatggtt
      121 ttttaccatg aaaggatgat cgaatatgac accaaatcga gaagattatt tgaaaatcat
      181 tttagaacta ggtggagata caacgaaagt caacaataaa caaatcgtat ctagtctaga
      241 tgtctcagct gcatctgtca gtgaaatgat ttctaaactt gtgaaagaag agcttgtaga
      301 acattcccct tatcaaggtg tacaattgac tgcaagcgga ctgcaaaaag ctagcagtct
      361 gattcgcaaa catcgcttat gggaagtttt tttagttgaa catttagact atagctggaa
      421 tgaagtccat gatgatgctg aagtattgga acatgtgact tcggaacatc ttgcagatca
      481 tttagaaaac tatttgaatc atcctgaaca ttgtcctcat ggaggatcga ttcctaaaaa
      541 agaggaagtt gtccatgaag aacgccgaca aacattggag tcctatcctg tcggtacaaa
      601 agtgcgtatc gcacgagtgc ttgatgaaaa agaattgttg gattacttag tcactattga
      661 cctgaatatc aacgaagaat atgagatcgt tgacatcgca gcttatgaag gaccgatcac
      721 tatccaaaat gatagaaaga aaatccccgt cagctttaag gcggccagca cgatttttgt
      781 cgataaaata taaaggagtg tatccacatg tctaatgaaa aaaatgtatt gtttacgata
      841 ttcggcggta caggtgacct agcccagcgt aaattgtacc cgtctttgtt ccgcctttac
      901 agaaaaggaa atttaggcga gcattttgct gtaatcggaa ccgctcgccg tccctggagc
      961 gatgagcact accgtgaagt tgtcaaagaa acaatccgtg cattgaatcc ttcagatgaa
     1021 gaagcaacta cctttgcaag ccatttttac tatcaatccc atgatgtaaa tgattcaacg
     1081 cattatcaga cattaaaaga attgtctgac cgattagatg gacactatca attaaacggt
     1141 aaccgtgtct attatttggc tatggcacct caattttttg gaacgatcgt ttctcatttg
     1201 aaatcacaaa atattatgac aaaaaatggc ttcaatcgtt tgatcataga aaaaccgttc
     1261 ggttctgatt accagtctgc ttttgaattg aaccagcaaa tcagagaagt attcccagaa
     1321 caagatattt tcaggattga tcattattta ggaaaagaaa tgatccaaaa catctcagct
     1381 atccgttttg ccaataatat ttttgaggct caatggaaca atcgctatat cgacaatatc
     1441 caaattactt ttggcgaaaa cctcggtgta gaagatcgcg ggggctacta cgatcatagt
     1501 ggcgcactta aagacatggt gcaaaatcat attttacagg ttgtagcatt acttgcaatg
     1561 gaaccgccag tagcattttc cgaaaaagaa atccgtacag aaaagatcaa agcattgaaa
     1621 gctatccgca tttataacga agaagaagtg ttagaaaact tcgttcgcgg gcaatatgct
     1681 caaggtgaat tggatggtgt acaattcaag ggctatcgcg aagaagacaa agtggatgct
     1741 caatcagaga ctgaaacatt cgtcgcaggc aaattcacta tcgataactt ccgttggtca
     1801 ggtgttcctt tctatgtacg tacaggcaaa cgtttgactg aaaaagggac tcggatcaac
     1861 atcgtattca aacaagttcc tgttaatgta ttcaaaacat ctgtcgatga accatgtgac
     1921 gatacgacac ttccacctaa tgttctaacg atctatattc agccaactga aggattttct
     1981 ttatccttga atgggaaaga agtagggcaa ggatttgaaa cggaaccaat caaattggaa
     2041 tttagaaata gtgcagaaat ggttgaaaat agcccagaag cttacgaaaa attactatta
     2101 gatgcgttga atggtgatgg aacgaacttc tctcattggg aagaagtcgc tcaatcttgg
     2161 catatcgtgg atgtcattcg aaaagcttgg gaccaaacac aaccggattt tcccaactac
     2221 aaagctggat caatgggacc taaagcagct ttcgaactac ttgaaaaaga cggattcgaa
     2281 tggatctggc aaccagataa ctggtatcgt gaacgtggaa aattaaacga ttgataacaa
     2341 aagggggtgt gacaaagaac atgtcacacc cccttttccc ttttactttt cttcgaatcc
     2401 ttcagctaca gcttcaggat agaagtcttc aacgattttt gcacaacgac cgcatagatg
     2461 aggaagtttt tcatctactc ctacatcttt acgaacttgg cgacagcgat cgcaagtttc
     2521 cccgtctgct ttttccactg taatagcaac atcatcaaat accatagcgt tatctggcac
     2581 ttgttcatct gctgccataa cttcaaagta gtctggtgaa acgatcaata attgtgcaat
     2641 atctgcatcc aaagctttta acatttcacg aacttgttgt tttggataaa cagttagttt
     2701 tgcttccaaa gatttaccaa tcaatttgct gtttcttgct tcttccaatg cttttaagac
     2761 attatcacgg aagtccatga aagcagtcca ggtatccatc aattcttctt ggtttgcaaa
     2821 ctcttcataa ccaggaaact cgcttaactg aacgtactcc tcttcttctt tcaggaatga
     2881 ccagatttct tctgctgtat gcggaatgat aggagtcaat aattttgtca aagctacagc
     2941 tgtttgatag aaaacagttt gcatgcaacg acgttgataa tcatcttctg cttcaatata
     3001 aactacatct ttagcaaaat ccaggtagaa tgacgataga tcaaccgtca agaagttcat
     3061 aactgtacgg taaatatgca tgaagttata tttgtcgtac ccttcttctc ggatttcttt
     3121 gattacttgg ttcaatcgaa cggtcatata tttgtctaca gaacgtaaat cattgtacga
     3181 aacagtatct tttttaggat cgaaatcttc tgtattcgct aataagaaac gcatcgtatt
     3241 tctgatttta cgatatactt ctgccacttg acttaagata tccatagaaa cacgaacatc
     3301 tgcttcataa tctacactag atacccataa gcgaaggata tctgcaccaa attgtttgat
     3361 cactttttct ggtgcaatga tgtttcctaa agatttactc atcttacggc cttcaccatc
     3421 taaagcaaat ccttgagaaa taactgattt gtatggtgct acaccattga tagccacact
     3481 agtcgtgata cttgagttga accagccacg atattggtct gatccttcta aatacatatc
     3541 tgctgggaat gataattctg gtcgctgacg taaaacagct tcatgagaag aaccagaatc
     3601 aaaccagaca tccatgatat ctttttcttt tgtaaattcg ccattaggag aacctgggtg
     3661 ggtaaatcct tcaggaagaa gttctttcgc ttctttttca aaccagataa ttgatccatg
     3721 ttcagcaaat aattttgcca catgttcgat tgtttctggt gtgatgattg cttctccatt
     3781 ttcagcatag aaaatcggaa gtggtactcc ccatgcacgc tgacgggaaa ttacccaatc
     3841 gccgcggtca cggatcatat tatacaaacg tgttttaccc catgggataa cccactctac
     3901 tttttctact tcatccaaaa tattctgacg gaatttatcg atagatgcaa accattgtgg
     3961 agttgcccga tagatcacag gctttttcgt ccgccaatcg tgaggataac tatgagtaaa
     4021 gaaatctagc ttcagcaaag ctcctttttc ttttaataga tcagtgatca taggatttgc
     4081 tttgtcataa aagatacctt caaatccagg tgcttcttct gtaaacaatc cttttgagtc
     4141 tacaggtgac actacagata gtttatattt gctaccagcg atatagtcat cttccccatg
     4201 tcctggagca gtatggacta atccagtacc agcgtctaaa gtaacatgat cgcctaaaat
     4261 cacaagtgat tcacgttcat agaatggatg acttgcagtc atatattcca tttcttgtcc
     4321 agcaaattct tttagaactg ttacattttc ccaaccgatt tctgattgga ctgtttctag
     4381 caaatctttc gctacgacaa acttacggcc atcggcttcc accaatacat acgtaaattt
     4441 tggatttaca gcaatacctt ggtttgctgg caatgtccat ggtgttgtcg tccaaatgac
     4501 aaaagctgta tcttcatcaa gaatatcttt gccgtctttg acattgaaag ctacaaaaat
     4561 agatggagat tttacatctt tatattcaat ttcagcttcc gctaaagaag actcacttga
     4621 cggagaccag taaattggtt tcaatccttt gtaaatatag cttttttcag ccattttacc
     4681 aaatacgcgt acttgtgcag cttcataaga aggatctaat gtaacataag gatgttccca
     4741 atctcctgca actcctaggc gtttgaaatc tgctcgttgc ttatctactt gggaaagtgc
     4801 atattcacga catttttcca agtattctgc catggacatt tctttacgtt tcacgccttt
     4861 attagccaat acttgttcaa tcggcaatcc atgtgtatcc catcctggaa catatggtgc
     4921 acgatagcca gacattgatt ttgaacgaat gataatgtct ttactgattt tattcaatga
     4981 atgtcctaaa tggatattac cattggcata tggaggtcca tcatgaagaa caaagctagg
     5041 ttttccttcg ttcaactctt gtcttttttg gtagattcga ttttcttccc attctttttg
     5101 ccactcgcct tctcggtttg gcaaattacc acgcataggg aattttgttt ttcctaattg
     5161 caaggtttct ttcattttca ttcaaccata cactccttta tttgttttcc ggacacaaaa
     5221 aagcatgttc agccgcaagg gacgaacatg ccgtggtacc acccaaattt agttgcatct
     5281 atctagtgca aactctctgt gaccaataac gaggtcagcc gctgcttctt actctaattt
     5341 cggaaaagca ggtctcaagt agaggatctt tcaccatgca gtgtcttacc gaacttccac
     5401 tctcatcggc tcgctttaag aataacaaag tgcttttctg ttctactcgt tttcattttc
     5461 accaggcacg atcgagatat caatagcctg tgtatcaaat gccgtcatag gtgcttctat
     5521 gactcctgtg tctgagttta catcagtatc attttcattg tcaagctgtt ttgctaaaac
     5581 ttctttgatc acttcatggc tgtcagtcac gtagctggag aatggttgta aaatttcatc
     5641 ccattccgga cttttcactt gttctaattg agactcaagc atcaagctga tacgttgatg
     5701 gaatactcgt gtttttttct taagatcttc agtttctgtc gcaagctgtc ttgctttttc
     5761 tgttgcatct gtcaagattt ttcttgcttt ttcttctgca tcagttgtta attgatgtgc
     5821 acgtttttca gcgttcgcaa ctaactcatc tgctttgttt tgtgcagaag taacgatcac
     5881 ttcggattcc ttgcttgcac ttgtttttac tttatcagcc gtatcttgag ctacgatgat
     5941 tgattggttc aaagcatctt ttaattcatt gaagtactca agtttttctt cagaatgttt
     6001 gactgctttt tctaattctc gatttctttg agtcaattcc tcatagtcac gaacaactaa
     6061 atctaagaaa tcatctactt cgtcttgatt atatccccgc atttttgtcg ggaatgtctt
     6121 attttggata tctaatggag ttaaagccat gagtaaacca cctttttatt ttatttacgt
     6181 aatacaccga acagtaccct gtacttttct tttttcgtct tcccctcaag ttcttggatc
     6241 tggactctgc cataacctct gactgaaata atatctaata gttcaagtac aaaatcaggt
     6301 cttgtatttt ctgtccagtt cacttttact ttaccagctt cgatcagctg cttggaccgc
     6361 tgtctggaaa tattataaat ctcagagatc acactgtcta gtcgcatgga actaactgtt
     6421 cctctttctt gtgtccagct atctttcggc ataatcattt ctgtgtattt gattggttcc
     6481 aagcgtacag caacctttcc aatctttgtt acttgtgcag caacaaaatg atccacctca
     6541 tttgcaataa atacttgcca gcgatcaccg tccgagatga tatcgccaaa gtaagagcgt
     6601 ttgatccccg ttcccactaa agttccaagg attttcccat gacttaggct ggcaaatttg
     6661 ctaggataat ggatttcata caatgctgtt tcaaaatctt cctctacagg ttcgtaataa
     6721 tcggggaaaa tcaaacatct ttttctttct gcctgttcat atccgccata aaacatgaaa
     6781 cgcaaatcac tgctttgtcg aatcagtgtt tctaaaatat aggattgtct tgggtcaaga
     6841 aattcggtca gatagggtgc atattgtcct tgaacctgtt caatccaatc gcctaccgaa
     6901 tcgataaatg tacgttcatc tgcccgaaag tgctggtata catttgcatc caaaattttc
     6961 ccctcctatc tattctgaga ataaagattt tgttgcttca atcaataaac taaatggaca
     7021 gttatccata taacgatctg tgctaaacct tgtacggcta agttcaagat caggattgcc
     7081 cctaaaattg tgaaatccaa gggtccaatc ctcaaaggca gacgatcgaa caaactgata
     7141 tacggttcgc agatcttagc taataatcgt cctagccctg attgataccc gcctggaaac
     7201 caagagagca atgcatatac catcaagata atgctataga aataggctgc ccgttgaagt
     7261 aaatgaagta atagtgcaat agtataaatc gtagttcctc ctcttacaga tcaaacaaat
     7321 tcgcatctgc aaaagattga gcggttgaac tgtcaatctc catcccagtc ggtgtacaaa
     7381 gaaaaatctc atctccaaca cgtttgatat ctccatctag tgcataaact gttcctgtta
     7441 gaaaatcaac gattcttctc gcttgttttt cttctactaa atgaaaattc actaaggttg
     7501 cttctccagc aatgattcgt ttagcaatcg ccatagcttc agaataagct cttggttcca
     7561 ttacagtgat ttttccagca agatttgctt taggttgtgt agaggttctt gaagctgttc
     7621 tttctttcgc tacagttttt gtaggtgatt gatgtagttc aattactttt ttcttttcag
     7681 ccggctgaat actttcatgc gttacaggac gtgaagtcgc cgttttttgt ttttcatgag
     7741 tattcgcagc agatacagaa tgagttgatt gattctgtgt tgtatttttt ttagttggct
     7801 gtacagcctt ttttgttgtt tcaggataat cttggtattc gtactcttcc tcatcagcta
     7861 atccgaaaaa attagcaatt gctcctctgt ttaaaagcgg catttttgtt cctcctttat
     7921 tcgtctttga atagcgctgt tcctacacgg ataaatgttg caccttcttc aatcgcaatt
     7981 tgaaagtctt gactcatccc catacttaat tcactacatg gtgcatgggc ataacctttt
     8041 gcttgaacaa gatcacgcaa ttctttcagc gtaccaaaca ctttatggat ttgctcatca
     8101 gaagcttctt taggcgccat agtcatcaac ccaacaacct tgatttttgt gcaggcagct
     8161 aaagattcca caaacggtaa aagctcatct ggtgaaattc catgttttga ttcctcacca
     8221 gaaacattta cttcaataaa acaggataat tctttctcta accgcttctg gatttcttct
     8281 gctaatctta aactgtccaa cgcatgaaag taatcaattt catttacaat caattttacc
     8341 tttctgcgct gtaaattacc aattaaatgc cactttattt caggaaaatc aaataaatct
     8401 tgtttctttt ctaataattt atctactcgg ttttctgcac aattcataac gccagctcta
     8461 accaattctt cagcttgagc ggattcaaca gattttgtta cagctactaa tgtaacttct
     8521 ttcggatcac gatgaacaag agcacacgaa tcctgaattt cttgcttgat tttctgcaag
     8581 ttgtcagcaa tcatgtgctc ttcctctcct ttatttttta cgacggaaaa acggtggcgt
     8641 atttaattca tcatcgccat gagattttgt ttcttcgcgg ttgaatgtat caaattcttt
     8701 tttttcgata ttgtcaaaat tggaatcatc aactctagga cgaacatttt gttcacgtct
     8761 gatatcccaa tcgccaaaac tgttttcttc ttctgaagaa gttggtttag cttgatccat
     8821 atctaataca gtcttttgag caggtgattg catttgtgct tgtctagctg gtcggctaga
     8881 cttgcgttcc ttttttgatt catcgatacc tgttgcgatg accgtcacac ggatttcatc
     8941 gcccatttct tcattgatag atgtacctag aatgatattt acatcgccag ttgctgcatt
     9001 tgctacgata tctgaagcat cttgtgcttc gaacaatgtc atatccaaac cgcctgtgat
     9061 gttaagaagt acttgttcag ctccatcaat agatgtttca agcaatggag aggagattgc
     9121 ttttttcgtt gcttctacta cgcgatcttc tccactagcc acaccgatcc ccatcaatgc
     9181 tgtaccttgg ttttccatca ctgttttcac gtcagcaaag tcaaggttaa cgtaacctgg
     9241 cgctgtgatc aaatcagaga taccttgtac accttgtcgt aaaacattat ctgcttcacg
     9301 gaaagcttca agcatcggtg tctttttatc tacaacttct aataaacggt tatttgagat
     9361 aattaataat gtatccacat tttctttcaa acgagcgata ccttctgcag caaaacggcc
     9421 acgtttcggt ccttcaaaag tgaatggacg tgtcacgaca ccaacagtca atgcgcccaa
     9481 ttctttcgcg atacctgcaa cgattggtgc agcacctgta ccggttcctc cacccattcc
     9541 tgcagtgatg aaaatcatat ctgcaccgtc taaagcttcg cgtaaggatt gttcactttc
     9601 ttctgccgct ttttgtccaa cttctggctg agaaccagca cctaaaccac gagtatattt
     9661 ggggcctaat tggataactg tttcagcttt tgaatttttt aatgcttgca cgtctgtgtt
     9721 ggctgtaatg aattcaacac ctttaacgtt ttcttcaatc attcggttta cagcatttcc
     9781 accgccacca ccgactccga tgactttgat tactgcgccg tcattgatat tattatcaat
     9841 tgagaattcc atatcttatt cctcccagga tgaattaatc aaaaatattt gtaaagaaac
     9901 ccttgatttt attttttaca ttttctccgg actcttttgg ttccggctca tcgtagattg
     9961 tttcttcagg tgcttcatag ctctcatatg tgtttgttgt ttcttgttca actaccattt
    10021 gacgagctgc tgaggtttca cccgtaacag caatttttgc caattgatac acttcactca
    10081 agtttgctga ataatccacg atacttatta catttgtaaa taccgggttt ctcagcccca
    10141 tgtgatttgg aacatacaat tttacattta cgccgaagat ttcttgtgcc aagtccacaa
    10201 caccaggaag actagcagct ccgcctgtca atacgacgcc acctggcaac tctaatgctt
    10261 cgatttggtc caatgcatct tttgctttgt tgaaaatctg ttccattcga gctgaaataa
    10321 tttctgacaa gtagcgttca tcgactttca ctggttctga ctgaccaatc acatctactg
    10381 gaaattcttc atctggtgat gtccgttctg ggtatgcatc cccgtagttt attttcaacg
    10441 cttctgcatt gttaaatgac gtatttaata caatagagat atctttagtg acaaattcgc
    10501 caccttcttg gtcaagattg gtaaacttca attgcttatc atgcataaca gccgttgttg
    10561 tttggccgcc acccatatca atgacgatcg taccaaaatc tttttctccg tcagaaagaa
    10621 ttgattcagt cagcgctaaa ggagtaatca ccatttcgtt gacgattaat ccggcatttt
    10681 ccacgcattt acgtatgtta tggatgatcg ttttaggtcc tgtaaacaac agaccgtaca
    10741 tttctaatct tacgccgatc attccacgag gatctttgat tccctcgaac ccatcgacag
    10801 taaaatcttg tggcaatatt gacacgattt gacgttcagg aggtattgaa cgaacaaggg
    10861 cagctgaagc cacatttctg acatcttcat cggtgatttc ctttgaatct ccgttaactg
    10921 caatcattcc ctggcaattt tctacttcaa gcatgtttgc tggcaaaccg acacttacgc
    10981 ctttgatctg gatacctgct ttttcttctg cttgtcgtac tgctcgttgt atcgcttgga
    11041 ccgttttatc gatatcaacg ataatgcctc ggttaatccc ttctgatttt gcgtttccta
    11101 ctccaataat attcatttgg ctgtcgatat attcagccac gacaacttta acagatgtcg
    11161 ttccaatatc aaggcctaca tacattcctg tttttgccat gaatgggatt ccctcctact
    11221 tactattcca tatacaagcg aacaactagc gtccgtttta aaatatactt attaatagat
    11281 atattcactg cttccaattc taccataaat tactggtttt tagaaagtac attcttactt
    11341 tttaaagttg ttttatcaat tttcatttaa acttgacgga gttgtacttg gattatcttc
    11401 tatttgactt gtttcacttg aatcatttga atcagatgat ccgtttgtgc tagacgatcc
    11461 aggtgtttgt gctttttgcg tttctgcctc ttcacttgat tcgtttgttt gatccgcatt
    11521 ttcttcactt tcagttgttt cttccgattg gcccgattct tccgtttctt gagtgctacc
    11581 gtaaggatgc gagaagatac caacttccat gtcgatcaca cctttttcat ccatatcttt
    11641 ggctacttgc gaataatatt tcatttggtt cgcaagatta ttgatattta caataacttg
    11701 attgccgtca ttcatattca attggatcag atttttattg cttgctcttg gcgtgtactt
    11761 gatttcagaa attgcttttt gtaattctga tgataactga ttgtattgct ttgttaattc
    11821 tttgattttt gcagagtctt tgaactcttc caagataggg agattttttg taggatttgc
    11881 tactttttca tctaatactg ttccattttc aatcactggg tagtattgat tgttttttgc
    11941 gatcaaagca atctctttat attctgttac atccaaatcg aattcattca tccctttgaa
    12001 atgtatattt gctgtttcga ttcttggctg tgctttttta aggcgatcga tataagtaga
    12061 tcgatgaaaa tactgtcccc aaacattttc tcctaatgac aattttgtat cagagatgat
    12121 ttcttggctg ttgactgtct tattgcctga tactgcaact gcttgtaatt tgctataagg
    12181 agaaacataa tagatcaaaa aaatcagcgg tatacccaat acagtgatga tcaaagctaa
    12241 ccgacgaaat agtactttgt gccgttgact ttttaaattg ggcagccggt tcaaaaaaga
    12301 accgttatat ggtccaccgt ttttcttttg cttgatctct tcaaaaaatt cgattttttt
    12361 ctcggactgg ctttcttctt gctgttcctc ttgaaccgtt ttttcttctt cagaatctga
    12421 aaggggtgta tctaatgtta tgtcttcttt ttttggctct tggtttttca tctcttgttc
    12481 tgccagatat tttcggttag ccttttgcca aggagtcaaa ttgttttcat cagaagacgg
    12541 atgagacaat ttttttggtt cttttttctt actcactaag accaccgcct ttattttttg
    12601 attatttgtt ttactaagtc ataaagacgt tttgaagcat cagggattcc ttgttcttta
    12661 gaagctcggc acatttgctt ttgcaattct tcatcaccca tgatttcgtt gactgtctga
    12721 cttagtgatt cacctgttag ttcattatct gcaatcattt ttgctgctcc tgcgtgaaca
    12781 agactcattg catttttagt ctgatgatca ttcgtaacat atgggctggg aaccaacact
    12841 gccggtaacc ccagagctgt gaattctgcg atcgaagttg caccagcacg ccccaccagc
    12901 agatcactgg aagccatgac ctctgccata tttttgatat aaggctggac actaatattt
    12961 tgtatttcag gatttatttt atttaggatc tcttcataat atctatctcc tgaagcatac
    13021 aaaacttggt aagaagaagg aatatattca cttaaaaaaa ctgatactgc ttgattgatt
    13081 ttaagtgctc cttgactacc gccaaaaatc aagactgttt tcttttctgg atccaaatca
    13141 aatgttttca aaatatctga ttttttagta tctgctactt cttgtgctcg aggattacct
    13201 acaagaactg ctttgttttc aggaaaaaag gaagccgcat ctttaaatga aagagcgatc
    13261 cgatccacat atcggcttaa aaatttattg gtaacacctg gtacactgtt ttgttcatgg
    13321 ataactgttg gaatacccat tttggctgca gcgtacacta ccgctccgga aacatatcct
    13381 ccagtaccga taacgatatc tggttgaaaa tcactgagga ttttttttgc ttgacggata
    13441 ctcttaagaa acaatcggac cgtcttgatg ttatgcatgg aaatttttcg tttaaatcct
    13501 tgaatctcca aggtatggaa aggcattcct gtatcaggga cgattttgtt ttctagacct
    13561 cgttttgctc ccacatacat gaattcagca ttcggttctt gagtctttac ataattgaca
    13621 aaggcaagag ctggataaat atgtccccct gttcctccgc ctgtaaccaa aattttcatt
    13681 ttttatcgct cgcttctact ttttctattt taatcggttc acagcttcca tgaatctgtt
    13741 tcctctagtt tcaaaatttg ggtattgatc ccagctggca ttcgctggtg ataataaaac
    13801 tacatcacca tctgtactgt tttctaatgc taaaggaact gcttgttcta cagaatcggc
    13861 tgttactatt gtctcgatgc ctgctttttt tccagcatcc atcaactttt cttttgtttg
    13921 gccaaatacg accattgctt tgattccttt taatgaagga attaattcat caaatgtatt
    13981 tcctctgtct aatccgccag ccaataacac gaccttagac gtttcaaatc cacttagagc
    14041 cattttagtc gctaaaatat tggttgcttt tgaatcatta taaaattttc tgccttggat
    14101 ttctcctaca tattgcgtac gatgtggtac gccatggaaa aaatgcaacg tttctcgtat
    14161 agcttcattt gatataccat acaattttgc tacagaaata gcagctagtg cattttctac
    14221 attatgctta cctggtacgc caagttcaga tatttccatt attttttctt ttttatagta
    14281 tattgagtaa tcgtcagcat acacaccgtc ttccaacact tcttttgtcg agaaaggcag
    14341 tacgcgtgct tttgttcttt gcgctaattc ttgtagttct gactgattcc agttaaggat
    14401 cagataatct ttttctgtca tgtttttttg aaggttccat ttagctttga catattcttt
    14461 tcttgtacca tgataatcaa tatgtgcttc atagatattg gtaatcaccg ctatatgagg
    14521 gcggaaatct gtgattccca tgagttggaa gctagacaat tccatcacaa ttttatcttc
    14581 tgattttgct tcttgcgcta caccacttgc tggataccca atattacccg ctaaacgtgc
    14641 aattccttga tctgctcctg cattcagtag tagtcctgtc atcgttgttg tcgttgtttt
    14701 tccgttcgtt cctgtaatcc caataattgg gcattccgat acttcgtaag ctagctcaac
    14761 ttccgtaatt accggaatac ccatttcttg ggcttttgct actaaaggat gactgtaggg
    14821 aatccctgga tttttgacca tcaatgaaaa ttcttcatcc aacaattcga ttggatgact
    14881 acctgttatt acttttatcc ctaaagaaag cagctcttgt gcttccggat tttcgtcaaa
    14941 aggttttcca tcattaacgg tgacaagtgc gcccaattca tgtaataatt tggccgcact
    15001 tactccgctt tttgcgagtc ctaagacaag cactttctta ttttcatatg ttgatatttt
    15061 tttcatttcc cgtcatctcc taccaaacga tccataatgt gatcaacgaa gtcacaatgc
    15121 tgatcaacca aaaaactgta tcaatcttcc actccgacca tccgcacatt tcaaagtgat
    15181 gatgaattgg agacatttta aaaattcgtt ttcctgtaag cttaaaggaa gtaacttgaa
    15241 gcatcacact tgctgtttcc ataacataga tcaaaccaat caaaagcaat gtccattctt
    15301 ggtttagcat tatagaaatt gccgctaaaa gtccgcccaa cgctaatgaa ccgacatctc
    15361 ccataaaaat cttggcaggt tttctgttgt agacgaagaa acccagtaaa cctcctaaaa
    15421 cacttaagca aatgattaat acatcatact gatgctgatg ccaagcaatt agaccgtaag
    15481 ctgcaaaaga gatacttcct aagccagcaa ctaatccatc gatgccatct gtcagattta
    15541 ctgcatttga aaatccaaca agccagaaga gagcgaaaat accataaatg attcctaaag
    15601 gtagatctag tccgaagaaa ttcaatacac ctggataacc ttcagaacgg taaacaagat
    15661 aaaagatgat tccccctaga atctgtccaa tcaatttctg cttcgagttc agtcccatgt
    15721 ttcttttctt gaatattttt ataaaatcat caagaaagcc gattactccg tataacgcta
    15781 atacaaaaag caagatgaaa agagtaggag ttagctgttt ttgccaagct ccaacccaaa
    15841 ttcctgtcaa aatagagccg atcagaaaca ctaagccgcc cattgtagga gttccggctt
    15901 tgctattgtg ccactttggt ccctcctcac gaatcgcctg accttgcttt ttcatttgaa
    15961 aataaccaat aaatagcggc attgcggcaa ttgtcattgc acaactgctg acaatgggaa
    16021 tcaatgcttg tgtccattcc atgctctttt ctctcctatt tctcttctta ttcttctaaa
    16081 gtaaaactaa gtttgtcttc agtgattttt tcatatggag caagttcttg tttgacacaa
    16141 taaccatctc catcaaaact aacttcaata ccgaggattt tccccagctt catgatatct
    16201 gcttttgacc atcctgttac gtcaggcatc agcttatcac caccagtata taggatcagt
    16261 ttttctccag agatcaactg atcaccgttg gcagtagatt gtttttgtac ttttttacca
    16321 ttaccgatca ctacaggttg cagtccactt ttttgagcat cagctgctgc aacgtccgct
    16381 tcaagattac gataatcggc tacggatact ttttctgttt ttgtttctgt atctgaatct
    16441 tcttcagttt ctttaaaatc cattgcccgt ttcatcaatg gatttgcgat tttagccaat
    16501 gccattcgat catatgtctt agggtgtttc attgttagat acaagacata gtctggatct
    16561 tcagaaggaa ccatctccac aatcgaatac aggtaggctg tatctcctgt ttgatatccc
    16621 cctgtatcag aagcgatttg ggctgttccg gtttttgccg atacattgta tccaggtaca
    16681 acaccataag cactaccgta gttttcgctt tcgacaacat ctctcatata ttcacggact
    16741 ttttctgttg tttcttttga aaaaggttgc cctagaactt ctgtttgagt tgttcgctct
    16801 tctccagttt ggggatccac cactttgctg atatatcgag gttgaatcat tgtaccgtta
    16861 ttcgcaatag aagtgaaagc tttcatcatt tggaaattcg tcacacctac cgcttgtcca
    16921 taagcactca ttgctcggtc gacaatattt aatgtaggta aagcaccgtt tacttcatca
    16981 tctaatccgg aatgagtact ttgtccaaat cccagttttt gtaaatagtt ataccatcgt
    17041 ccgcctaatc gctgttccag tatgaccatc ccgacgttac ttgaccatga aagagcttga
    17101 cgcatggtca gcacaccttt ttcaccaaag tcatggtcat tgatcgtagc atcttctact
    17161 tggatttttc cagattggaa ggtttcattc tcgttgaatt ctccttcttc gatagcagct
    17221 gcagtagtga aaactttcat ggttgatcct ggttcataac tatcttgtac caagaaatta
    17281 cgccagatag catcttttcc agtcaatcct tccattgttt caggattgaa agtaggtcgt
    17341 tgtcccattg ccaagatttc tccggttttt gctttcatca ggactgctgt caattcttct
    17401 ggctgatatt cttcatttac ttgatccatc agtgtttcta aataactttg tagtctgctg
    17461 tctaaagtcg tgtagatatc ttgtccgtcg acagcttttt cttcttctgc cactgtacct
    17521 ggcagaggat tttgaaaatt atctttttga taaataatct taccatcttt accgctcaag
    17581 atatcattgt aagcagactc caaacctagt ttaccaacta agccattctc atctttgtct
    17641 ggaactgcgt atccaataaa atgagaagaa aagacgccat ttggatacat ccgagcctga
    17701 tggtcgacaa aataaagacc agcgacacct tcttttttca tgtccgcttc gatgttttgc
    17761 ttggtttctt gggagatatt tttcccatag ctaccaaatt caacttgata cagctctttt
    17821 tttgcgccgt cctctaaaat attcagtgca tcttttttgt ctatagaaag attcttatgc
    17881 aggatttctg ctattttatc aaaattcttt tcttcaacat acagtttttt atccccagag
    17941 gtatatgttt ttgagagtat cgctttaatt gaataagagg aggcatcttc tgcgagcgcc
    18001 acaccgttac gatcataaat cgttcctctt tttgctttta ctacttcact tccttggtac
    18061 aactgttttg ttttttcagc taatgaggta ccagcaacat gacctccaat aacgatatac
    18121 gagaaacgga cggcaaataa aaagaacaat ccaatactcg tagcaaataa aatgatacct
    18181 acttttttgc gattgttcat cggatttaga tttttctttt ccataaaccg ccgaatttta
    18241 tttttaagac tcatttactt cactttcctt aaattgtcgt cattaataga caatcctttt
    18301 tcttctgcga tttgtttaat ccgttcagta cgagaaagtt cgtttttttc ttgttctaaa
    18361 ctcgtttttt cttgcttttt atcattgatt tctgtttgta ttgacgtgat ttctttttca
    18421 gctccgctga tatttgtgcg cagcataact gttaaaacac ataaagcaac aatagcaatc
    18481 gccacgcttg ccacagcaat tttttccaaa gcagaaatct gctttaatcg ttttgccggt
    18541 gattgtggaa gaatgatttc ttgattcgaa gaagaatcag gcatttgttg ttccggctga
    18601 tccaccaatt caggtaaatc atattcgtaa tcttgcattt tctttaattc tgccatcatt
    18661 ctactcctct ttcattctta ttttttctgc aatacgtaac tttgcactgc gtgatcgatt
    18721 gttttctgtt agctcttcct ctgaaggaag gattggttta cgagtaatga ctttcagttc
    18781 tggttggaat tcgtcaggta tgacaggaag tcccggtggt agatcttgta ccgtactata
    18841 ttctttaaac atacttttga caattctgtc ttccaatgaa tggaatgtga ttacgctgat
    18901 tcttccattg actttcaata accggattgc ctgttctaaa gaggcctctt cggcaccgag
    18961 ttcgtcattt actgcaatcc gcacagcttg aaaaattctt tttgcaggat gcccgccttt
    19021 tcttcttgcg ggagctggga tgacttgttt aatgatttcg acaagctcac cagttgtttg
    19081 gattggtttt gtttttcgta ctcgttcgat ttcacgagca atttgtttgg aaaatttctc
    19141 ctcgccatag cggaagaaaa tctttactaa ttcatggtag gaatattcat taattacgtg
    19201 ataagcggat aaaggagctg actgatccat gcgcatatcc aacggtgcat cttgatgata
    19261 actaaatcca cgttctgctt catctagctg aggagaggaa acccctagat catacaaaat
    19321 gccgtctacc ttatctacat gctcactcaa gcgttcttct aattctctga agttcgcttt
    19381 gataaatgtg accatacctt gttcgacata agaagctaaa cgcagcttgg cgtgatcgat
    19441 tgctttttga tcttggtcaa acgcatacag atgcccttta gaacttagtt ggcttaataa
    19501 atattcacta tgtccggcac cgccgagtgt acagtctaca taaacgccat ccggtttgat
    19561 gttcaatcca tctaccgttt ctttcaacat gactgtatag tgttgaaatg tttcagtcat
    19621 tagtcagcct cttcctattc ttatagtccg aaatcaatca tactttcagc aatttcgtca
    19681 aagttctctt ctgctgctgc agaaaaatct tgccatcgtg tctcatccca aatttcaatt
    19741 cggttagata caccgatgac tacgcaacct tttgtcaaag ctgcataatt tcttaacgtg
    19801 ctgggtatat tgattcgtcc ttgtttatca agttcgcatt cagtggcagc cgaatagaag
    19861 aatcgcacaa aagtacgtgc gtctttttta gccaacggca tttcgttcag tttagcttcc
    19921 agattttccc attcgctcat cggatagccg aaaagacagc catccaatcc gcgtgtcaga
    19981 acaaactttt cgccaagttc ttcacgaagt tttgagggca cgattaaacg tccttttgca
    20041 tcaatattat gttggaattc acccatcaac attgaaagcc ccccattcct ccactaacta
    20101 aaattagtct accacaatcc cccactttct accacctttt ctgggaaatc cgggaacaac
    20161 tttttttatt cataaacatt tttttaaaaa tcaagctgca aaaagccttt taaaagactt
    20221 tttacagctt ttctattttt ttcataggta agaaagtatg ttcacaatga tcaagacagc
    20281 gtacaagacg attgtcaaca gaaaaaccaa acgccaagtc atcttgaaat aacgcggata
    20341 aataatttct tcataataat atgcttggaa aacagcaata caaattccca ttagcaaaac
    20401 agaaataaag aaaaacggta agatagaaat tgtccctgta tttctagaca atgcatgcat
    20461 accgccccac aaaaaaggaa tcgccaaatc aggggctttt attttaaaac gacgatttaa
    20521 cgaaaataat tttacaagaa attggcaacc aaacaaaacg attaccgggt aaatatacca
    20581 aaacaagata agtggtgtga atgtgcccat gaacgtaaac tcctttttta tcgttgcttc
    20641 aagtatactg tatatacttt acacaactgt ttcattttat ttaaatttat gcagttatat
    20701 tctatatctt acaaacacat tccatgtatt ccaattccct atccgttata ataaaagaaa
    20761 caggcaacag gaggaaaatc atgatcaaat attttacatt agataatgag aaactgcgta
    20821 ataccggaaa ggaaactgac caaacgatct ggtattgtgt agaacgtcct aatcaagaag
    20881 agattcatca gctgacacat caatttgaaa ttccgtcaga ttacatcact tccatattgg
    20941 acgatgcaga aaattcccgt acagaagaat tcaatcaatc aaagttgaca aagccagcgt
    21001 tattattgtt acaatatcct tttgctaaaa caagtcctag cggttatttt caagtggata
    21061 catacccgct ctctattatt ttgacgccca aaaagaagct tatcacaatt acgaatcatg
    21121 aaccactgtt tttaaagaaa gtctttagcc agacctttcc aaaaagtgat atttcgccta
    21181 atctcaatat cttctttcaa ttcatttggc aggtcacact ctcttacaac agttatttag
    21241 ctacgcttca atctcaatgc gatactttgg aagccaaact tcaagtatcc actgaaaaca
    21301 aacaacttta tcaaatcatg gatatccaaa aaagcctcgt ttatttcaga gaagcgactt
    21361 ctgctaattt agatgcattg aaagaactag cgcaaaataa acttttccaa gaaaatcatg
    21421 ctttgaaaaa tcatttacgt gatgtattgg tggaaacaga gcaggcaatg actacttctc
    21481 gcattcaatt aaaactagta gaacaaatga atcagacttt ttcagcaatc gtatcgaata
    21541 atctgaataa tattatgaaa attttaactt ctttaacgat tattttaact ataccgacaa
    21601 tcgtcggcag tatctatgga atgaacatta agttacctat tgctgaaagg gatgatgcct
    21661 ttatatggct agttctatta acattattta tttgtatcgt aactacctat tatttaaaaa
    21721 aagggaagtt tttataaaaa agccctcttg tggaaatgga aaaaagattg tacacttgaa
    21781 tcaactaatt aatgacgagg ttgaaaacaa tgcgtgaaaa aaaacaaaca agtacttttg
    21841 ctaaagtaac aaaatttgtc atttggacaa tgctgatttt aacgattgga tctgttgtat
    21901 tgactgctgt aatgagtgtc atgtaaaaat aaaatatagc tatcagctgc tgaaaaggac
    21961 ctgaaccact gcgaggtggc tcaggtcctt tttgcgagtt tcaatagttc ttttgcatgt
    22021 tcgagtgtga gttttgtaat ctcacttcca gccagcatac gtgcaatttc ttcttctctt
    22081 tcttttgttt tcaactctga aacagaagtt tgcgtacgtc catcaatcac tgctttcaca
    22141 atataatatt gatagtcggc gactgcagca acttgaggca gatgagtgat gcacaaaact
    22201 tgagagtatt tcgatatttg agaaatttta tctgcgatgg cctgtgcaac tcttccgctc
    22261 acacctgtat ccacttcatc aaaaataata cttgtcacac cttgctcaga agaaaaaata
    22321 gttttcaaag caagcaacat ccgagacagt tcgccaccag aagcgacttt caccaaaggc
    22381 ttcagtggct ctcctggatt ggtcgtaatg taaaattcga tttggtcaaa tccatcatga
    22441 ttcaattgtc tattttcttc ttttaagaaa cgtacctcaa actcagtgtt ttccatatac
    22501 agacttttca attcatgcag aatggatttt tccaattctt ttgcgatttt tttacgcgct
    22561 tcatgaagat ctgcggcttg ttgctgaagt agaagctctt tttgctctag ttcctctttc
    22621 attttgtcca attgaccttc tgaaaaatca gaagaagcta gctcttcggt gatttcgtca
    22681 tagtaagcca atattgactc aatggattcg ccgtatttac gtttcaactg tcggattaat
    22741 tctaaacgtt gcgtaacttc ttctaaacga ctctcatcta actccaataa atcaatctga
    22801 cgactcatat cgccgattgc atcttgcagc aaatagtaag cactttggat attttcataa
    22861 attgcttcat actccacatc tagatgggcg atcccttgca gttctgaaac ggccaagcca
    22921 acaccatcta cactgttttg ttcattattc gtcagtgctc cgtaacctgc agctaatcca
    22981 tcagcaattt tttgataatt gctaagtttt tctctttctt cttttaattt ctcttcttct
    23041 ccgacttgca aatcagcagc agctatttct tcttgctgga aatggagcat atctattctc
    23101 tgaacatagg atttttcatt ttgctgcact tttcgtaccc ttttttccag ctcacgataa
    23161 gagatatatt cttgctgata actctttttc ttctgttgga aagctacatc tccaaaacga
    23221 tccaataaag caagatgtga ttcaggctgg agcagttcct gatgttcgtt ttgtccttgg
    23281 atatctacta aataactgcc gattcttctc agattagcta atgtgataat atgaccattc
    23341 actcgacaaa cattttttcc agttaaggac atatcccgtc gaacaatcag attgtcctca
    23401 tctgtttcga tccctaattc aaccatcagt tcagagaatc cttcttgttt tggtagttca
    23461 aacaaccctt caagcacaca tttttctgct ccttgacgta tataatcgct tgaacctctt
    23521 cctcctgcta acagacctaa agcatcgata ataatggatt tacccgcacc agtctctccg
    23581 gtcagcgcgg tcattccttc gtgaaaagaa agccgcaatt ccggaataat cgcaaagtta
    23641 gtaatactga tttcttgtag catcacattc gctcctcaac ttaaaggtaa tgcaggaagt
    23701 ctttcttcaa taaaactgcc tggtcttcag ttcgtgtaat catcaaaaca ttatcgtcat
    23761 cattgataat cgaaaataat tctttcttgt aacgtttatc aatcaaattt gctgctgccg
    23821 aagcatttcc cggcaatgtt ttcaaaataa caaatttttc catttggtca actgccacga
    23881 aagcgtcttt taatagtttt tccaatttag tatttacatc ttcgtttgtt tcagctggta
    23941 tgctataacg gtagccgcca tctgaagccg gtaccttgat cagtttcagt tcctttatat
    24001 ctctggaaat cgtagcttga gtaacagata ttcctttgct tttcaggatt tcaacaaatt
    24061 cttcctgctt tcggatatct tgctcactca acaatcgagt aatcaaacga tgccgatctt
    24121 tttttctcat tacgtcttcg ctccttagca tattcttctt aatcaatgat agcttatttc
    24181 cttggtataa taaagaagaa tccgcatact tctcacttga ttttcacagt tacaatttat
    24241 ttatggtcaa actgttcgtg tgcatttgaa accacttctg ccatattaat agctgaattg
    24301 attgttccgc tttcgggtac tttcctcaag tgagccaaga actcgatatt tccttctccc
    24361 cccgtgatcg gagaataact taattccagc acatcatatc caccttgtgc cgcaaatgac
    24421 aaaatgtctt ctaaaacttt ttgatgggtc tgagggtcac gaacaattcc gtttttccct
    24481 acagcttctc tgcctgcttc gaattggggt ttgattaaag caatcacttc tccctcttct
    24541 tttaaaatag catgtaatgg aggcagcatc agttttagtg aaataaaaga aacatcgata
    24601 acagcaactt ctggaattcc taaggtaaag tcttcgggtt ttgcataacg aaaattcact
    24661 ctttccatta cgacgactct aggatcttgt cttattttcc aagctagctg attgtagcca
    24721 acgtctaaag cataactcat ttttgcgcca ttttgcaaag ctgcatcagt aaatccgcca
    24781 gtggatgcac caatatctaa caatgttttt ccttcaacag atagatcaaa tgctttgagt
    24841 gctttttcta attttaatcc ccctcgagaa acataaggaa gttttttacc tttgactctt
    24901 aattcactac tcactggaat tttttctccc ggtttatcaa accgctcgtt tttttcgttg
    24961 taaatcaaac cagccatcac tgaacgtttg gcttgttctc ttgtttcaaa taatccttgt
    25021 ttgaccgcta atacatctac gcgttctttt tccattcttt ccctcatttc tccacatcaa
    25081 attgcttgat catctggcgc atcagcccgc catcaaaaga aaaaacgtcc tcttctaatt
    25141 tgtctatgat cttgttggcg gataacagct caatctccaa cgcctcacgt gtcttttcta
    25201 ggcctaataa acgcgggtaa gtattttttt ccatacgttc atctttacca gctgttttcc
    25261 ctaatgtttt cgttgtacca atcacatcga gtagatcatc tcttatctga aaagctaaac
    25321 ctaaatgttc ggctaatcgt tgaagtagca ataagatttc ttcaggttgc ttcgctaata
    25381 tgccaccagc cagcaaggca taacgaatca aatgccctgt ctttctttca tgaataaatg
    25441 ctaattcttc taaagaaagc tttttcgatt cgccctcgat atctgcagct tgcccagcaa
    25501 ccataccttg tgatcccgca caaacagcta attgctgaag cagcagaagc ttaggagaat
    25561 tacctaagtg tgccatactg attagctgga atgctcctgt aagcaaacca tctccggcca
    25621 atatagctaa tgcttcgccg aatactttat gattggtagg ttttcctcta cgaagatcat
    25681 cattgtccat cgcaggcaaa tcatcgtgga tcaaggaata cgtatgcacc atttctaaag
    25741 cagctgctac ttgatagacg ggaacatcaa taggctcatc aaaggaagca acggccgtta
    25801 aaagtaataa gggacgtatt cttttcccgc ctgctttcac tgaataactc atcgcatcat
    25861 agagtcctct atccgttgta tgctcatcta aaaaagacaa gatttctttt tctacagcag
    25921 gtagatgaat agctgaaaag tcagttaatc tcatctcagc tttcctcgtt tccgtcaaaa
    25981 tcaacttctt catttgattc tgtcatcatt ttcgtcaagg tctcttctgc tttagctaat
    26041 gtatcttggc aatgtttgct taattccatt cctcgtttaa acgaatctag agctgattct
    26101 aatggaacgt ctccttgttc taactgcatg acaatttttt ctaattcttg cagtgactct
    26161 tcaaaagttg gattttccat atttttattc tcctttgtct atctcttgta cttgtccttt
    26221 aacgattcca tcttgataat ggatcgtcag caaatcccct tgatgaatct cggtgacact
    26281 ttttactact tgatcattta acgtcgtata gctgtagcct cttcccatga ttttcaatgg
    26341 actcaacaaa tcaagagatt gaacagcttg agcaaaacgc tgctgctgtt gtttcatata
    26401 ttgttggatt ttctcttcta agcgtttttc tagataagca gtttcttggc gagctgcttt
    26461 aactcgataa agcggagcca cttgttccaa ttggtggctt aatgcgatcg tttgtttttc
    26521 tttcgcatga taaatttgct gtgtagcttg atataatcgc tgtttcaact ggtctaattt
    26581 gatggtctga ccttcataca atctttcagg ctgccgaaat acataggaac gtcgagcacg
    26641 ctcgaaccgt tcttgtttgc gctggatctg gcgtacaaac gcctgctgca atctagcttg
    26701 ccgttcttga atgcgcatca actcttctga caaaacagga acagctaatt ctgcagctgc
    26761 tgttggagtg gcagcccgaa catctgctac taaatcagcg atcgtggtat ctgtttcatg
    26821 tcctacagat gaaatcaccg gcgtattagc ttgataaatc gcccgagcaa cccgctcttc
    26881 attaaacggc cataaatcct caatcgaacc acccccacga ccgatgatca ttgtatcgaa
    26941 attgcccatc tcttctactc tctggatatt cctgacaaca ttgtccgctg cttggtttcc
    27001 ttgtacaact gttgggaaca aaaccaattg gacaattgga tatcttcttt tcaccgtcgt
    27061 gatgatatcg cggataaccg ctccgcttgg actggtaagg accgcgattc gctttgggaa
    27121 acgtggcagc atttttttag gcccttgaaa aaggccttcc ttcatcagct tttcttttcg
    27181 ttcttccaat tcttgatata aagcacctat tccatcaggc tccatgtgct ctacatagat
    27241 ttgataagaa cctcccgctt catataatga aatacgccca acgacaagga ctttcattcc
    27301 ttcttttggc tggaatcgta atttttggaa tgctcctttg aacataatag cagatatttt
    27361 tgcatggtca tcttttaagc tgaaatattg atgggcattg gcacgtaaac ggaaattcga
    27421 tatttcgcct gttaaatata cgcgttctaa ataaggatcg gcatcaaatt ttcttttcaa
    27481 atacttcgtt aatgtagtga cagttaaata ttcttgtgcc atctattcac ctgctccaaa
    27541 agcttcggca gattttacgg tttgatacat cagcatcgtg atagtcattg gccctacacc
    27601 ctttggcaca ggagtgatcc agcctgcaat aggctccact tcatcaaatt tcacatcacc
    27661 gatcagctta ccgttagcat cacgattcat ccctacatct atcactacag cacctggttt
    27721 tacgaattct tttgtgacaa aatgtccacg tcctattgct acgactaaaa tatctgcttc
    27781 ttgtgccagt tcagccagat tttcggtttt agaatgagca acagtaacag ttgcatctgc
    27841 catcaagagt agctgagcca tcggtttacc gacgatattg cttcgtccga taactactgc
    27901 tcgtttaccc gccggatcga tagaataagc ttcaaacatt ttcatgatgc cgtatggagt
    27961 acaaggaatc ttgtccggtt tacccgcaag gagtctgcct aaattcatcg gatgaaaacc
    28021 gtctacgtct ttttcagggc ggatagctaa caggatcttt tcttcatcta tatgctttgg
    28081 caatggtaat tggaccaata taccatgaaa actaggatct tgattgtatt tttcaatttc
    28141 tgcaagtagt tcgtcttcag aaatcgtctc aggataacgc tctaccaatg aatggattcc
    28201 gatttctttt gctacacgtt ctttatttct tacatagatt tggctggctg gattttcccc
    28261 taccaataaa acgactaatc cgggatggat accattatct ttcaattttt ggattttttc
    28321 agcaatctct gcctgcattt tttcagctaa ttcttttccg ttaatcaatt ctgccatcta
    28381 accactccca gatatttttt gtgttttcat tctaccatag aattttcccg atttataaca
    28441 ggtttatgat gattcataga aaaaagctag gacttacgcc ccagcttcta attctttcaa
    28501 tacattggat aaaacaccgt tgacgaattt acgtgaacgg tcatcactaa atgtttttgc
    28561 caactcgatt gcttcattta aagcaacagc cgctggtaca tctgtcacat aagtcatttc
    28621 aaaaatcgcg atacgcaaga tcaccaaatc catttttgag atccgattga tgtgccaatt
    28681 gttttttaaa tgtttttcaa tcactttatc caattcatct ttttttgcac agacaccgcc
    28741 aactagcgta tcaagataaa caggaacaaa ctctgattca tcttcgttga tcatgtcgcg
    28801 gtggtctaat tcgattgcgt tgaaaatcgc atcttctttt gttaggtctg cattgaagtc
    28861 taatggaaat aaagcctgca atgccatttc ccgaatctcg tgtcttgata attctttatt
    28921 cattctcttc ctcttcgtct tcaaaaagtt cttcgattgt tggttgttct aatttttcag
    28981 gaacaacagc tactacatgg atattgactt cagataaatc gatatccgtc atgaaaagga
    29041 cttgctggct gacccgttcc tgcatttcca ttgctacttt aggaactgaa acaccatatt
    29101 ctagataaca gtaaagatct actttcaagc catcttcctc gtttgctaag taaacacctt
    29161 ttccgtgagc tgcacgtcct aataattcag tgacattgct ggcaaaagtt ccgcgcattc
    29221 cgtatacgcc ttctacttta gaagctgcaa tcccgataat tacttcgatg acttcaggag
    29281 cgatcacgat ctctcctaaa tcttggtttg cgtttaaaat caaatttttt tcatctgcca
    29341 tttatttatt cctccctttg cctaagcacg agatacatcc gaattctcta tgatgcggca
    29401 tagtgatcac gtggtaagaa aactgccgcc gatcagctgc aaactgctat gaattggaga
    29461 aaacacccaa ctttattatc tcttacagct taaaagttct tctatagtgt atcattaaat
    29521 cgagtgattt tctataacaa agtctgtgaa cactgatttt tacgcttact tcttctattg
    29581 atgaaactat aagatgatca attcttttgg tgaacgagtc aacacacgat ttccatcttc
    29641 tgtgatcaac aagtcatcct caatacgtac tccaccgatt cctggtaagt agatccctgg
    29701 ttcatcagtg atcacatttc ctggtacaaa ttgcttttct gcacgagaag aaacgtttgg
    29761 tccttcgtgg atctctaaac cgatcccatg tcctgtagaa tgtccaaatg cttccccgta
    29821 accatgtttt gaaatataat ctctagcaac tgcatctaat tggacaccag tcattcctgg
    29881 ttttgcagca tcaataactg ctaattgagc atctaagact atttgataaa tttcttttaa
    29941 tttttcccca ggatcaccaa tagcgaatgt acgtgtcata tcggaaacgt acccttcgta
    30001 atagcaacca aagtccaatg tgatcaagtc accttgttcg atgatttttt tgcttgctac
    30061 accatgcggc atagccgaac gcaatccgct agcaacgatc gtatcaaaag aaacacctga
    30121 agcgccaaga gaacgcatat aaaagtctag ttgatttgcg acttcaatct cagtcatgcc
    30181 cggttggatc attttcagga tgtgatcata tgccatatcc gcaatatgac atgctttttc
    30241 gataatagcg atttcttctt catctttcac ttctctgagt tcttcgatca tctgtgaaac
    30301 aggtactaat tccgcatcga tgatttcttc taaaaccgaa tattctaaaa atgagactgt
    30361 tgtttcctca aaagctaatg agtgcagttc ttctttgcgt actaagtttg ctacctcatc
    30421 aaaaataggt ccgacatttt taataatttc aaacccttga gcctgcgctg ccgcctgctc
    30481 ggtataacga aaatcagtaa taaaaaatgc tttttccaaa gtaataacag ctaatcctgt
    30541 cgtacctgta aagttcgtta agtaacgaag attgtacgga ctggtaacca aaaatgaatc
    30601 caaattttct tcttgcattt ttttgcgcaa tttttctaca cgtagcatca taatgaaact
    30661 ccctctttct ttgcacgctt ttttccatta tagcaaactt ttcttcgtac acgacagtta
    30721 aataaaaatt tccggaaaac ttctttttat tttaaataaa taaagccaaa aaggagtcac
    30781 agtctcgctg caactccttt tagtttacta ttttgtaaaa acttatgatc gttttccaaa
    30841 gaaaaatgag acaactgcta ccaaaatcac tgcgcctaaa atagatggaa taagtgccat
    30901 acctgcagct tttggtcccc attcgccgaa aatcgcttgt ccaatacttg aaccgactag
    30961 tccagcaatg atattcgtta cacagcccat cgaatttcct ttactggtga tcgcacctgc
    31021 aattgctccg atcacggctc caacaatcaa tgtccataac caactcataa tataacctcc
    31081 ttctttgcta atcaatttta gcatatggga tttaaagggg acaaataatt ggttttgcta
    31141 tttcatattt tcttctgcaa agtaaaaaaa taaagaaaac ctcctcttag aagttttctt
    31201 tattttttac ttgcgtttaa acatactacg aataccacgt ttcactacat caaagaaact
    31261 caatgattgt accattctat cttcatctac atattctctg atcgcacgaa gcactttttt
    31321 aggttctttg gaagaaaatg tgtatgtacc attttttttc gtttgaatgg catatcgagg
    31381 aatccatttt ccctttagca tgactgatgc tagtacataa tccacttctt cccatggaat
    31441 ttggatatat ttgcgtctat ctcgcgcatg gtaaaactca aaaccattat cacctatcat
    31501 gatttgcccg taatctgtca gacccgtaaa cgaagtagca tcaattacca agtctacttt
    31561 tgtattgatt gattgaacca ttttctccct ccagatattt gtttatgaac agtatactat
    31621 ataaaaaaag ctcgggacaa cgcccgagct atatttttat ggattataaa agtccgatga
    31681 cgtgtccaac aacaccgact acgaataaac caaggatgat aacgattgga gaaacctttt
    31741 tcttaagcaa ccacatacat aacaatgtta atcctaatgc agctaaacca ggaatcaatt
    31801 ggtctaagtt gtcttgtaat gttgttacct tttcaggaga aagggctaag ccattattaa
    31861 cttgttcaaa tgcactcttg attccttcac cacctgaagg caatttatcc cattcgatgt
    31921 aagcaccttt atctaatttt acagttgata caactggtaa gaatttgatt gatacccatc
    31981 tttgtacaag ggcagctaat acgaacatcc ctaaaattga agcacctttt gttacgtctt
    32041 gtaacaatcc gccagaaagg tcatcagtaa ttttagaacc agctttgtag ccaaattctt
    32101 gtgtatacca catgaatgcc caacgaataa tgttccaacc taagaagaag atgattggtc
    32161 caaggatatt tcctcccata gctaatgaag cacctagtgc acctagcatt ggacgtactg
    32221 taaaccagaa cactgggtca ccaacaccag ctaaaggacc catcatacct actttaaccc
    32281 cttgaatcgc tacatcatca acaggcgcgc cattcgcacg ttcttcttcc agagccaatg
    32341 taacccctaa aattggagaa gcaatgtatg ggtgtgtatt aaagaattcc aagtgacgtt
    32401 ttaatgctgc agaacgttct tcttttgttt tgtataattt tttgattgct ggaatcatag
    32461 aaaatgccca gccaccgttt tgcatacgtt cgtagttcca tgaaccttga atgaaggtag
    32521 agcgccacgc aacagctaaa cgatcttttt ttgataattt gattttttct tctgccattt
    32581 tattttcctc cttcaagatt aatagtcatt caaaatgtcg cctaaagggt caccagagtt
    32641 gcttccgcct ccattatttg atgaaccacc cattttagaa aggttcaagt aaataagagc
    32701 taatgcaaca cctaaagcac ctagagcaat caatgttaat tgagaaattg cagctacaac
    32761 gaaaccaata acgaagaatg gccatacttc ttttgtagcc atcatgttga ttaccaaagc
    32821 gtaacctaca gctacgacca ttcccccacc gatagccata ccatctgtta accatgctgg
    32881 cattgattca aggaatgatt gcacagtttg tgctgggata aatagcaacg ctgctgctgg
    32941 gaccgcgata cgaacccctt gtaaacatac agcaatgatg tgccacattt caaccttacg
    33001 gatgtttcct tcttcagcag ccgcatccat caaatgaacg attggaacag cgattgtacg
    33061 tacgatcatt gttaagaaca accctgcaac agccaaagga acggcaatcg cgatcgcaga
    33121 aggaacacct ttaacacctt gtccacctaa aactaaaata attgctgatg ctactgatgc
    33181 taatgcagca tctggtgcta cagcggcacc gatatttgcc caacctaaag cgatcatctg
    33241 aagcgtaccg ccaagtacga taccagcttc gaggttaccg gtaactaaac cgatcaatgt
    33301 acatgccact agtggttgat gaaattgaaa ttcatcaaga ataccttcca taccagctag
    33361 aaaggcaact aatacgacta aaattattga aataatagac atgataagcc tcctataaat
    33421 tttaatatta tttattacgc ttttgctaat tcagcttttg cttttttcaa aatatcatcc
    33481 atgttatcgc gtgaatcatt aggtacttta cgtacatcga atttcacacc tttttgttca
    33541 agcttctcaa acgcttcaac gtcttcttgt cccatagaca atactttact tacaacgact
    33601 ttaccgaccg aatgcgccat tgacccaaca tttaattctt tgatgtctac gccaccttca
    33661 acagctgtca atacgtcttc aggattttca aataaaagca atgctttggt atttccaaaa
    33721 cgagggtctt ttgctacttc gatcattttg ctaataggaa taacatttgc ttttacacct
    33781 ggaggagcag cttgttcgat tagtctctta cgcagatcat cctttgatac tgcatctgaa
    33841 acaacgataa tacggtttgg ttgcactgct tttgtccaag ctgtcgcaac ttgtccatgt
    33901 aacagacgtg agtcaacacg tgctaataca tatttgattt ttccgtctcc gaccacagtt
    33961 ccttctggaa gtgctccttt aggttgtgcg tcttcaactg ctgctttcgg tgcttctgcc
    34021 ggttctaatt cttccggttt tacttttaca ccgtcttttg ctgtttcaat gatgtgtgct
    34081 gcaatttcat gagcagattc cattgaaaag cgtgatgcgt aggcttcaat caacattggt
    34141 aaattcaaac cgctgacgat tgcccattta tctttgtgct cttcgaataa tgtgtttgat
    34201 tgattgaacg gtgttccgcc ccataaatca actaagaata acacttcgtc ttgattatca
    34261 aaagaagcaa ccgcttcttc caatttggct ctcaagtcat ctggaccttc acttggtttc
    34321 aaaatcacgg ctttgacgtt ttcttgttcg ccaaagatca ttgaaccgga ttgtaagatt
    34381 ccttcagcaa attgtccatg acttgctagg ataatcccta ccatatttat acctcctatt
    34441 tacttttgtt gtaacaaatt caaaaatatt aataattgct aaccattaca acagaattat
    34501 ttgacctttt ccaacaacca ttcttgctac acctttttga ttttcgctag tagttccatc
    34561 aaaaggattt tcctgtcagc aggtactttt cgaatctcta actccacttc ttgactgttc
    34621 aagtaacgaa aggaatcaat atcttgttga tttactgaaa caaaattggt aatcattgat
    34681 ttgccttcag taaaactcat accgccgata ttcaacgact tcaactgtac accgcagcgg
    34741 acaaccgctt caacatcttg gggattggtg aataaaagca tcacttttgc tcgtgataat
    34801 aatccactat gatagatttg aatcattttc tgcacggtta ttacatttgc tgtgatcccc
    34861 ggcggtgcag cttcttttag tagaaacttt ctcaatttat cttgagcgac tacatcgctg
    34921 atcacaatga tacgttcaac acctgtttgc ttcgaccaaa ctgttgcgac ttgcccgtgg
    34981 attaaacgat catctatacg tgccaatcga atatccatct gcaaaactcc ttcttcctca
    35041 ctacttcaca gaaaccccta tcatttctgt gtgaacaacc acctcctagc acacactact
    35101 ataagcaaaa agcgtgccaa ctttttttac gatacactaa ttgaatttaa aggattctta
    35161 accaatacac aaaaacgata cactataaaa gtgtatcgtt tttgtgtatt catggcacag
    35221 tgtattcttt tttatctaac tgattatgga ctagacgtag cagataatac atttcttcta
    35281 tcggcacttt tacttttatc tgatcttcca acagaacaac tgaggctgcc aattggctat
    35341 aaagaggatc attcgttgta tttgtcaatt cgttttcttc cacagtcaat ggttcattcc
    35401 gaataatacg ttcgatcatt cctgctgtgt gcatcactag gttgattttg aatccatatt
    35461 cttcatcgcc gattcccgtc gtctgagaga tttgtgtaga aaattgccac attggatcga
    35521 ttaacttact accattaata aagatgaagt tatcagagat aaactcaaca cacgtatttt
    35581 tagctgactc ttcatctagg gagatgaatt cagtttcctc caattctgat tccatcagca
    35641 actgatccaa aatgacttcg atattttgat caataaaacg ttctaaagga ataaatgggg
    35701 cttggatatt caaatcagta atcccagtag tagctatgat ctgatattct ttttggataa
    35761 ctggcagttc ttctttcact tccacaatcg acaaagctag aacttctaat tctgtttctt
    35821 gacgcttgtt taccgctcgt tcgatcaatt ctttgattct ttgtgctgta ccttcgccag
    35881 atgcgcagat agccaaaaca gcttttttct tatgaatagg ttttgcttcc tgaacagatt
    35941 ctacagaaga tatttctgcg taacctcgaa aatttttcaa tgattcatgc aacatatcta
    36001 gatctgtccc taatactgag acctttcgta cagtttccaa aactagagaa gttgttacca
    36061 tatcaacagt acgtactgga attccagtgt ctctttgaat ttgcgaatta aacgacgcaa
    36121 gggaccccat atctactaag agtatgaccc cgcttccttc atctacttct tgtacattgc
    36181 gttcaattcg gtccaatgct gttttcggat ccatatccaa aggcatgtct actgctcgta
    36241 cgttctctgc atctaacaac tgttggacaa cttggaccat actgcttgct gtgctgtttc
    36301 cgtgagctgc aacgactaca ccgattctgc cgcttgcttg atttgcccgt aacgagacga
    36361 gtagaacggt caagtaatag tcttcacttt ctggaatctc cacttgaaaa tacgtttcga
    36421 tataactacg cacttctttc gcaacagcat attcatctgg aaaatcaaaa gccatctcac
    36481 ggatgttatc attcgtatgc cgttcttctc ctaagttgat ttttttcaaa aaagaactga
    36541 tatgcaagct catagcataa ataaagtttt gctgaaactc atatggtaaa gatcttttga
    36601 ccattacata aatctgattg gtcacttcta tgattttcgg atctacaaat tctgctaatt
    36661 tgctgtctgc attaaaagaa aaaccatggt ctttatagaa agacttcaga tggatgttga
    36721 tatctgtaga aatgaaatga ttgattgctt cttgatccaa accatccgac ttcagtaaag
    36781 ctgctttatc tccgatgata tcatatagat tataaggtaa ctcataggag tccgattgga
    36841 ttttcatgat cgtatcattt ggtgctacag ttatctttgg ctccaatatt ttagaaagct
    36901 ctgccattgc actgcggttg cttgctaatt gaatcaatcc gctacgaatc ccttctgtca
    36961 aatcatcgat tgtgatcgag atttcgggtg attgcatatg atttagaaat cctcgtgcac
    37021 atactaactg gatatttgac ttcaattgtc ctatatttcc ataggtcaca ctgccgatca
    37081 atgcttttgc tacatcttct gtcaaagaaa tctttcgctg cgtgcggttt gcttcgtggg
    37141 caatcatgat ttttactaaa tcgactttct catttgcagg acgtttttca aaagccggaa
    37201 gtttgatatt gattgggata cgccgaacaa aagtttctaa tagagaagaa cctgggtctt
    37261 ctgtggtcgc acctacgatc cgtacatttg cttcatggga tttcgttgtt tccccaagac
    37321 gactgtaagt tccatgatcc ataaagtaaa agatcatttc ttgaccttca ggcggcaacc
    37381 ggtgaatctc atccaaaaac agcataccgc catccgcttg atcgataatc cccatttttt
    37441 cttcatctgc gcctgtaaaa gcaccttttg cgtacccaaa taaatgactc atcaaaagct
    37501 cagggttatt ggcataatca gcacaattga aaacaatcag ttccgtttcc tcatctatta
    37561 catgatgcgc ttttgcaaaa tgatacatcg cgtgtgcaaa ataagtcttt cctgatccgg
    37621 taggtcctgt aatcaaacaa tttaatcctc ttggcggata caaaatcgca gcttttgcct
    37681 gttccactga atttttcatg ctgccgttag ctccaatgat ttttgcaaag atatccttag
    37741 tatctatttg gagtgtattt ttttcttcag agaccggcgt ttctttgtaa ctgactacat
    37801 gttttgtaac tggctttacg tgcgtaacga gtgtttgata tatgtaacgt actggtcgtc
    37861 cgtctgtctt tatcagtttt ccttcgcgca ctagctgatt cagatcttta ctgctatttg
    37921 ttcgctgaat ccctaacact tcagcaattt cctgtgttgt tactccttga tcatattctg
    37981 cctttgttaa atgagcggtt ttttctttga catacatata aattcggtca atgcgttttg
    38041 ccaatggtat cgctcctttt ctatagacta ttgtacttag gcttcaaaac ataatactat
    38101 gtatttattt ctgtttcgtc aactatttac ctgattttgt tttgttgata acacgcggat
    38161 agatagaaat gttcaattta aaagaacgaa aaatcccgat acgattttta taatggaaac
    38221 aagaaaaaga acactgttta ccatataatt atttcactga tagcaaacaa tatagcttcc
    38281 atcaattgaa aacgatagtt atcaaaacat cctgattcca aattggcaaa aaaatattaa
    38341 gtgtaaaaag acaaagtaaa
//
LOCUS       DAHHZJ010000009        35753 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.009, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000009 DAHHZJ010000000
VERSION     DAHHZJ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.009"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(282..1451)
                     /locus_tag="NBL02_000355"
     CDS             complement(282..1451)
                     /locus_tag="NBL02_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCD2571070.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
     gene            1816..2004
                     /locus_tag="NBL02_000356"
                     /pseudo
     CDS             1816..2004
                     /locus_tag="NBL02_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604486.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            2050..3015
                     /locus_tag="NBL02_000357"
     CDS             2050..3015
                     /locus_tag="NBL02_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD2571071.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            3037..3249
                     /locus_tag="NBL02_000358"
     CDS             3037..3249
                     /locus_tag="NBL02_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HCD2571072.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            3324..3800
                     /locus_tag="NBL02_000359"
     CDS             3324..3800
                     /locus_tag="NBL02_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HCD2571073.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            3797..5605
                     /locus_tag="NBL02_000360"
     CDS             3797..5605
                     /locus_tag="NBL02_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HCD2571074.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            5682..5897
                     /locus_tag="NBL02_000361"
     CDS             5682..5897
                     /locus_tag="NBL02_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family protein"
                     /protein_id="HCD2571075.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            5989..6852
                     /locus_tag="NBL02_000362"
     CDS             5989..6852
                     /locus_tag="NBL02_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571076.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            6889..7425
                     /locus_tag="NBL02_000363"
     CDS             6889..7425
                     /locus_tag="NBL02_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HCD2571077.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            7439..7861
                     /locus_tag="NBL02_000364"
     CDS             7439..7861
                     /locus_tag="NBL02_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HCD2571078.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            7791..10190
                     /locus_tag="NBL02_000365"
     CDS             7791..10190
                     /locus_tag="NBL02_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HCD2571079.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            10222..12213
                     /locus_tag="NBL02_000366"
     CDS             10222..12213
                     /locus_tag="NBL02_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCD2571080.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            12236..12487
                     /locus_tag="NBL02_000367"
     CDS             12236..12487
                     /locus_tag="NBL02_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HCD2571081.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            12477..13706
                     /locus_tag="NBL02_000368"
     CDS             12477..13706
                     /locus_tag="NBL02_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HCD2571082.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            13703..15784
                     /locus_tag="NBL02_000369"
     CDS             13703..15784
                     /locus_tag="NBL02_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HCD2571083.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            15933..19853
                     /locus_tag="NBL02_000370"
     CDS             15933..19853
                     /locus_tag="NBL02_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HCD2571084.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            19854..20798
                     /locus_tag="NBL02_000371"
     CDS             19854..20798
                     /locus_tag="NBL02_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571085.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            20802..21005
                     /locus_tag="NBL02_000372"
     CDS             20802..21005
                     /locus_tag="NBL02_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HCD2571086.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(21305..21541)
                     /locus_tag="NBL02_000373"
     CDS             complement(21305..21541)
                     /locus_tag="NBL02_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD2571087.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(21724..22110)
                     /locus_tag="NBL02_000374"
     CDS             complement(21724..22110)
                     /locus_tag="NBL02_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HCD2571088.1"
                     /translation="MDARAMSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECI
                     CVQSISYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAA
                     CSKRMRRRKEAERQRKRYWKPTHLEL"
     gene            complement(22092..23420)
                     /locus_tag="NBL02_000375"
     CDS             complement(22092..23420)
                     /locus_tag="NBL02_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HCD2571089.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(23381..23710)
                     /gene="mobC"
                     /locus_tag="NBL02_000376"
     CDS             complement(23381..23710)
                     /gene="mobC"
                     /locus_tag="NBL02_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HCD2571090.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(23968..24339)
                     /locus_tag="NBL02_000377"
     CDS             complement(23968..24339)
                     /locus_tag="NBL02_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD2571091.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            24484..24669
                     /locus_tag="NBL02_000378"
     CDS             24484..24669
                     /locus_tag="NBL02_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCD2571092.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            24827..24994
                     /locus_tag="NBL02_000379"
     CDS             24827..24994
                     /locus_tag="NBL02_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571093.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            25612..26271
                     /gene="vanR"
                     /locus_tag="NBL02_000380"
     CDS             25612..26271
                     /gene="vanR"
                     /locus_tag="NBL02_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HCD2571094.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            26274..27617
                     /gene="vanS"
                     /locus_tag="NBL02_000381"
     CDS             26274..27617
                     /gene="vanS"
                     /locus_tag="NBL02_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HCD2571095.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RSDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            27788..28597
                     /gene="vanY"
                     /locus_tag="NBL02_000382"
     CDS             27788..28597
                     /gene="vanY"
                     /locus_tag="NBL02_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HCD2571096.1"
                     /translation="MEKKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGW
                     RLASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIP
                     AQYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKI
                     AEYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSY
                     RFGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            28615..29442
                     /gene="vanW"
                     /locus_tag="NBL02_000383"
     CDS             28615..29442
                     /gene="vanW"
                     /locus_tag="NBL02_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HCD2571097.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYPIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            29439..30410
                     /locus_tag="NBL02_000384"
     CDS             29439..30410
                     /locus_tag="NBL02_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HCD2571098.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            30403..31431
                     /gene="vanB"
                     /locus_tag="NBL02_000385"
     CDS             30403..31431
                     /gene="vanB"
                     /locus_tag="NBL02_000385"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HCD2571099.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            31437..32045
                     /locus_tag="NBL02_000386"
     CDS             31437..32045
                     /locus_tag="NBL02_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HCD2571100.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDKLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(32111..32284)
                     /locus_tag="NBL02_000387"
     CDS             complement(32111..32284)
                     /locus_tag="NBL02_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571101.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
     gene            32633..33064
                     /locus_tag="NBL02_000388"
     CDS             32633..33064
                     /locus_tag="NBL02_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCD2571102.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
     gene            33071..33304
                     /locus_tag="NBL02_000389"
     CDS             33071..33304
                     /locus_tag="NBL02_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD2571103.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            33728..33928
                     /locus_tag="NBL02_000390"
     CDS             33728..33928
                     /locus_tag="NBL02_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCD2571104.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            34012..35205
                     /locus_tag="NBL02_000391"
     CDS             34012..35205
                     /locus_tag="NBL02_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCD2571105.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            35509..>35753
                     /locus_tag="NBL02_000392"
     CDS             35509..>35753
                     /locus_tag="NBL02_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCD2571106.1"
                     /translation="MEVIIMNVGFIGAGKVGCSFGKYFQEHKIQVTGFYSKSEDSSLA
                     ASNFTSSKQYLNLRELVDENDTIFITTPDGQIQEVWQ"
BASE COUNT         9221 a         8956 c         9533 g         8043 t
ORIGIN      
        1 ttattatcca tggaagtaaa gacttttttc ttattgaaag tgaacagaaa ataattgata
       61 caagaaatca ggatttgaac acgctaataa aaatattgtt agtaaaacta tacgaggtag
      121 tcttttcgga tgcagagatt gtttaaatat ttgatagaat agaaacttaa aaagtttaat
      181 aaaactcaat tatataagta gtagatgaat atgatggtta tttagattat ggtagcgtca
      241 agaattatat gtaaatggaa aatccattct gttaattaca gttaaatcat cgactctaat
      301 gtatcctgaa tttctttaaa tcccttgtga attctaccta aaaatttttg gttatattga
      361 ttaaattgag aaatgaagaa tctctctaga gattcttcat tagggaattg ttcttttctt
      421 cgagtatatc gttttagctg cttattaaag ccttcaatca ggttcgtcga ataaattgtt
      481 cgtctgatgg cgtgagggaa attataaaag gttaataggg caggattcaa gagtaaattg
      541 acgactcttg gatactgctt tttccatttc ctagtcataa agtcgatgtg atccaatgcc
      601 tcttcttttg aattcgcttg gtaaaccaat ttgaaatcct cacaaatttc ttttcgacct
      661 cgaacacgaa ctttatgagc gatatttcta gatacatgca cacaacattg ttgaaattgg
      721 gcattcggat acacactatg gatactttct tcaatacagc ttaatccgtc tgtcactact
      781 aataaaactt cttctaaccc acgttttcta agatcttgaa gaacttcttt ccaaatataa
      841 gcagactcag ttggggcaat agtaaaccca agaacttctt tggttccatc caaacgaata
      901 ccaatcgtaa tatacactgc ttccttcgaa acagtctgtc gcttcacagg gatatgcgtc
      961 gcatccataa atataacgga ataattggct tctaacgttc tttctttaaa agctaaaaca
     1021 tcttcagcca ccaatttact catgttggag attgtttgtg gcgtatagtg gtgaccatac
     1081 attttttcaa ttaatttgga gatttcagcc atagtaatgc ctttttgaaa taattggata
     1141 atggtcgttt ctaatgaatc attggttcgc ttatatgctg gtagtgtttg ttgagaaaat
     1201 tcgccattcc gatctctagg aatagttaaa ttcaactcgc catattctgt tttaaatgta
     1261 cgtgaataat tcccgttacg ggaatttcct gaattgaatc catttcgatc atatttttcg
     1321 taatctaaaa aagcagttag ctctgcttgt agaagtgaat taatcgctcg ctctaaatga
     1381 tcctgaaata attcatctaa atccccttta ttgagtagtg tttgcataat ttctatagta
     1441 aatttagtca tgagaaagtc ctcctataaa atttctgtgt ggtaacttta attttacaga
     1501 atggactttt ttttctaccc taaatttcta tttacacaaa atattttacg ctatctttta
     1561 tatcaaaatt ttaggttatc gctgaaagcc ccggaaatac gggcatttcg caggatatga
     1621 gaactcaatt tactaccaat ttactacttt cggattgagc cgcatatatg ctgaaccata
     1681 ctgctaatac ccgccacccc aaatacggca catcataaat tgaatacgac accgtaaacg
     1741 cggcgttcct ctttcccgtc atgggagaac aggacgccgc ttttttatgc ccaaatagag
     1801 aaaggaggcg acgccatgct atttacttcc ggccctgacc gggcgtttga acggctgatg
     1861 caggaaaagc ccggtttcga tcactacgac ggcggcgggg cggacgcgcc ggaggattgc
     1921 ggcacctgcc gattttaccg cccggattgg aaatatcaat tctgccagtt tgcagagtgt
     1981 ccctatcagc cgggcaagct gaccgactat gcagcggtca aattcacagt gaaaggagcg
     2041 aacaacgcta tggcagtttt ccgtgtggaa aagaacaaag gctatacggt gatgtcaaat
     2101 caccacctgc ggaataagaa cctgacctta aaggccaagg gcctgctgtc gcagatgttg
     2161 tccttgccgg aggattggga ctatacccta aaaggactgt cctatatcaa ccgggaaagc
     2221 atcgacgcga tcaggacagc ggtatgggag cttgaaaaag ctggatatat ccggcgtgag
     2281 cagggccgtg acgcaaaagg caaaatggcc gatatggtct ataccatcta cgaacagccg
     2341 gtattggaaa acccggtatt ggaaaatcca acatcggata acccggtgtt ggaaaatccg
     2401 acatcggata atccggcgtc ggaaaatcca acgcaattaa ataaagaagt acaaagaact
     2461 gacttatcaa aaaaagaaaa aataattaca gatggacaaa gtacccattc cattcctatc
     2521 ccatccccga cccctgcccc tatggagggc gaggcagcgg ctacgccgcc ggaacggaaa
     2581 aggacaggaa aggatgaggc tgtacggatt tatcgggaaa tcattttgga gaacatcgag
     2641 tatgcctatc tcattcagga caggagcatt gaccgtgaac agcttgacga aatcgtagac
     2701 ctgatgctgg aaaccgtctg caccgcccga aagacaatcc gtgtcgccgg ggacgactac
     2761 cctgccgagc tggtgaaatc caagtacatg aagctgaccg gcgaacatat ccgctttgtc
     2821 cttgactgtc tgcgggagaa cacaacgaaa gtccgcaata tcaaacaata cctgcgggct
     2881 atgctgttca acgcccccag caccatttcc aactactaca cggcccttgt cgcgcacgac
     2941 atggcacagc ccgattgggg aaagcctaaa tccggcctgc cggactattc ctgcggcgag
     3001 ggcgaaagcc tgtaacgaga aaaggaggac gaccctatga agcagggcgc attgattttt
     3061 gacgagcgaa cagaccgtta cgacatccgc tttgatctgg ccgactatta cggcggcctg
     3121 cattgcgggg agtgtttcga cgtgatgatc ggcggcaaat ggaggcccac ccgcattgaa
     3181 atggccgaaa actggtatct tgtcggcatc cgggccgacg acctttccgg cctgcgggtg
     3241 cggatttgac ctatatctac ctgcggcgtc tggtggttcc gccttagacg ccgcttttga
     3301 ctttctgaca aggagggatg cgattgcagg aagaagttac tcaaaagacc attgccctgt
     3361 ccatgaaaac gggcaagctc acggctcaag tgctgcaagc ggcgctgaaa aagtacctgc
     3421 aacaccgggc caagggcaaa aacacgctgc accacggcca gcagagccta aagcagctta
     3481 aaaagcatgg ggctgatctc tccaacattg agatcaccga ggccaacatt ggagcgttca
     3541 agccatgcgc caagaaatac ggcgtggatt ttaccttacg aaaagataag accacccagc
     3601 cgccccacta cgttgtgatt ttcaaggcca aggatgcaga taaccttgat ctggcgtttc
     3661 gggagtttac cgccaagacg ctttccaagg agcagcggcc ctctatccgc aaggttttgt
     3721 ccgctgtgaa gcaaaaggcg gcggcccaga ccaagcagcg ggccaaggag aaaatcaagg
     3781 aaagggggct ggaactgtga agcctgacgt aaagaaattg ctgatcctga acctcccgta
     3841 tctgattttt gtgtacctgt ttgcgaaatg cggggaggcg taccggctgg ccgctggtgc
     3901 tgacctgtcg gggaagctcc tgcattttgc ggatggtttt gcggcggctt ttgccaaccc
     3961 gctgccaagc ctgcacctgt ttgacctgtg tatcggtatc gccggtgccg tgatcgtgcg
     4021 gctggtggtg tactgcaaga gcaagaacgt gaagaaatac cgcaaaggca tggagtatgg
     4081 cagcgcccgc tgggggacgg ccaaagacat agcgccttac atcgacccca agtttgaaaa
     4141 taatatcctg ctcacccaaa cggaacggct taccatgacc ggcaggccca aagaccccaa
     4201 gacagcgcgg aataaaaacg tgctggtgat cggtggcagc ggttcaggga aaacgagatt
     4261 tttcgtgaaa cccaacctga tgcaatgctt tccgaccacg gactatccga cttcgtttgt
     4321 ggtgacagat ccgaaaggca ctttggttct ggaaaccggc aggatgttcc agcaggcggg
     4381 ctaccgcatt aaaatcctga ataccattaa cttttccaag tccatgaagt acaacccctt
     4441 tgtctacatc cattcggaaa aggacgtgct aaagctggta aataccctga tttccaacac
     4501 caagggcgag ggggaaaagt cagcggagga tttttgggtg aagtcggaac ggttgtttta
     4561 ttcggccctg atcggctata tctggtacga ggccccagcg gaggaaatga acttctcaac
     4621 gctgctggaa atgataaacg ccagtgaagc ccgcgaggac gacccggagt ttcaaagccc
     4681 cgtcgatctc atgtttgagc gtttggagca gaaagacccc gaccattttg cggtgcgcca
     4741 gtacaagaaa ttcctgctgt ccgcaggcaa gacccgttct tccatcctga tttcatgcgg
     4801 tgccagactt gcgccctttg acatccggga ggtacgcgag ctgatggagg acgacgaaat
     4861 ggagctggac accatcgggg acgaaaagac ggtgctgtat ctcattatga gcgatacgga
     4921 cacgaccttt aatttcattc tggctatgct ccaaagccag ctaatcaacc tgctttgtga
     4981 ccgggctgat gatgtgtacg gcgggcggct gcctgtccat gtacgcctga ttttggatga
     5041 atttgccaac attggccaaa tacccaattt cgataaactc attgccacca ttcgaagccg
     5101 tgaaatatcg gcctctatta ttttgcagag ccagtcgcag ctaaaagcga tttacaagga
     5161 cgccgccgag atcatttcgg ataactgcga tagtgtgctt ttccttagtg ggcgcggcaa
     5221 aaatgccaag gagatttccg atgcgctggg gaaagaaacg attgacagtt tcaacaccag
     5281 cgagaaccgg ggaagccaaa cgtcccacgg attgaactat caaaaattag gaaaggagtt
     5341 gatgtcccaa gacgaaattg ccgtgatgga cggcggcaag tgtatcttgc agctacgggg
     5401 tgtgcgcccg tttttatcgg agaaattcga tattaccaag catccccggt acaaatacct
     5461 tgccgacgcc gacaagaaga atacctttga cgtggacaga tttttgacta tcacgcgccg
     5521 gaaacggcag caagtggtaa cgcaggatga agtattcgac ctgtatgaaa tcgacctgtc
     5581 ggacgaggac gccgccgctg aataaacagc ggcttttttc attccaacaa caaaatcaaa
     5641 aaatcattat gagccggaaa ccggcagaaa gaggtaaatt tatggcattt tttaattccg
     5701 cagttactac tttgcagact atcgtgatcg gactgggcgg cgctctgtgc gtatggggcg
     5761 gcatcaatct gttggaggga tacgggcagg acaatccggg ggcaaaatcg cagggtgtca
     5821 agcagcttgt cgctggcggc ggcgtggccc tgatcggtgt cactctggtt cccctgctgt
     5881 ccgggctgct gggctgatcc tggcctttgt ccctgtccaa acccttaact aatcgcctcc
     5941 gcgtcctccc ggccagaccg ggagggcgcg ggaaaggagg tgctacccgt gattagtgaa
     6001 atcattgaag aatggataaa aggcatcctc atggacggta tcacgggcaa cctgtccggg
     6061 ctgttcgata cggtaaatgc caaagtcgga gaaatcgccg ccgacgtggg acgaaccccg
     6121 caggcatgga acagcggtat tttcaatatg ctgcacagcc tgtcggaaac cgtgatcgtc
     6181 cccatagccg gggccatact cgcccttgtc atgtgctatg agcttatcca gatgataacg
     6241 gaaaagaaca atatgcacga ctttgacagc tccatgttct tccgctggat attcaaatcc
     6301 gcgtttgcaa tcctgatcgt aacgaacaca tggaacatcg tcatgggcgt attcgacgcc
     6361 acccaaagcg tcgtcaatca aagcgccggt gtgattatcg ggcagacatc cattgatttt
     6421 aacaccctga taccagacct tgaaagccaa ttggaggcta tgagcgtggg cgctcttttg
     6481 gggctgtggt tccaaaccct tgttgtgggc ctcaccatga atatcctttc catctgcatt
     6541 ttccttgtga cctatggccg catggtggaa atctatgttg tcacgtcgct ggggcctatc
     6601 ccccttgcca caatgggcaa cgcggaatgg cgcggcatgg gccaagggta tctgaaatcc
     6661 ctgctggcgc tgggcttcca agccttttta atcatggttg tggtgggtat ctacgcggtg
     6721 ctgatacaga atatcggttc cgcttccgac atatccggcg caatctgggg ctgtatgggc
     6781 tatacggtgc tgctgtgctt ctgcctgttc aagaccggca gtatatcaaa ggccgtattc
     6841 ggggcgcatt gatcccaaaa tccaagcata cacaggagga acttgtttat ttcaacgtac
     6901 aacatcattt acgctgaccc gccgtggcgg tatgactgca aaaacgtaca gggcgcagcg
     6961 gaaaatcact attccaccat gagcattgac gagctgtgcg ccctgccggt ggaacggctg
     7021 gcgtccaaag actgcctgct gtttttgtgg gccacattcc ccatgctgcc ggaagctctg
     7081 cggttaatca aagcgtgggg cttttcattc aagaccgtgg cttttgtatg gctgaaacag
     7141 aaccgtaaaa gccttacatg gttttacggg ctgggccgct ggacaagggg aaacgcggaa
     7201 atctgcctgc tggcaacaca cggcaagccg aagcgtcgtt ctgcctctgt ccatcagttt
     7261 attatcagcc cgattgaaca acacagcaaa aagccggatg tgacccgtga aaaaatcgta
     7321 gagctggcgg gcgatttgcc ccgcgtggag ctgtttgcca gacagaaaac ccccggctgg
     7381 gacgtgtggg ggaacgaggt ggaatgtgac cttatcctga cgtgaaagga ggtgccaaat
     7441 ggcttatgta accgtcccca aggacttaac ccatgtgaaa agcaaggttg tgtttgggct
     7501 taccaaacgg caactgatct gtttttccgg ggcgctggtg atcggcgtcc ccttatattt
     7561 tctcacccgt gactatctta ccaacagcgc ggcggccctg ctcatggtat ttgccatgct
     7621 gccgggcttt ttgcttgcca tgtacgagcg tcacggccag ccccttgaag tggtgattat
     7681 gcagatcatc cagtgctgct tcatccagcc aaaggagcgt ccctatcaga ccgacaacac
     7741 ctacaccgcc cttgtgcggc aataccagat ggaacaggag gtaaaggcca ttgtccaaaa
     7801 aggaaatccc aagagaaacc gaaaaaacaa agctgacccg cgcccagcga aaagaaattg
     7861 acgcggtaat ccgtaaatat aagggcgacg gcaggcccca cacggcgcag cagagcatcc
     7921 cctacgaggt catgtacccg gacggcgtgt gccgggtatc gcccggcgtc ttttccaagt
     7981 gcattgagtt tgcggacatc agctatcagc ttgcccagcc ggacacgcag acggctatct
     8041 ttgaaaaact gtgcgacctg tataactatg tggacgcctc cattcatatc cagttttctt
     8101 tcctcaatcg gaaagttgac ccggtgcagt acgccaagag ctttgagatc gcgccgcagg
     8161 gggacgactt cgacgacatc cgggccgagt acaccggcat tctgcaaaag cagcttgcaa
     8221 acggcaacaa cggcatggta aagaccaaat acctgacatt caccatcgag gccgaaagcg
     8281 tgaaagcggc gcgagccaga ttgaagcgca tcggctttga cctgttaggc tatttcaaga
     8341 gcatgggggc cgtagcccat gtgatggacg gctgggaacg gctgaacctg ctgcatggcg
     8401 tgtaccaccc ggacggcgag atattcaatt ttgactggaa gtggctggcc ccgtccggcc
     8461 tctccaccaa ggattttatt gccccgtcct ccctgtgctt cggcaatgca aagacattcg
     8521 gcatgggcgg caaatacggc gcggtaagtt tcctgcaaat cctgtccccg gagctgtcgg
     8581 acgatatgct ggccgacttc ctcaatacgg aaagcggtgt tttagtcaat ctccatgtgc
     8641 aggccattga acagaccaag gcgatcaaga ccatcaagcg caaaattacc gaccttgacg
     8701 ctatgaagat tgcggagcag aaaaaggccg tccgcagcgg ttacgatatg gacattcttc
     8761 ccagcgacct tgccacctac ggcgaggacg ccaagaagct gctgacaaag ttgcagaccc
     8821 gcaatgaacg gctgttccag ttgacgttcc ttgtcctcaa cgtggccgac accaagcaaa
     8881 agctgaataa cgacgtgttc caagccgcag gcgtggccca aaagcataac tgtccccttg
     8941 tccggctgga ctaccagcag gaacagggcc ttgcaagcag cctgccgctg ggcgtcaatc
     9001 agatcaagat acagcgcagc cttacgactt ccagcgtggc cgtgttcgtc ccctttgtga
     9061 cgcaggagct ttttcagggc ggcgcggcca tgtattacgg catcaacgcc aagtcccgca
     9121 acatgattat gctggacagg aagcaggccc gttgccccaa tgccttgaag ctcggcaccc
     9181 ccggcagcgg caaatccatg agctgtaaat ccgagatcgt cagtgtgttc ttaacgacgc
     9241 ctgacgatat ttttatttca gacccggagg ccgaatatta cccccttgtc aaacggctcc
     9301 acggacaggt aatccggctg tcgcctacca gcaaggactt tgtgaacccg ctggacatca
     9361 acctgaatta ttcggaggac gataacccgc tggccctgaa atccgacttt gtgctgtcgt
     9421 tctgtgagct tgtcatgggc ggcaagaacg ggctggaagc cattgaaaag accgtgatag
     9481 accgggctgt gcgcgtgatc taccggccct atctggcaga cccccgcccg gagaatatgc
     9541 cgatcctgtc cgacctccac aaagcgttgt tagatcagca tgtcccggag gctgaccggg
     9601 tggcgcaggc ccttgacctg tatgtgtccg gctccctgaa cgtatttaac caccgtacca
     9661 acgtggacat cggcaaccgg cttgtgtcct ttgacattaa ggagctgggc aagcagctca
     9721 aaaagctggg gatgctcatt gtccaagacc agatatgggg gcgcgtcacg gccaaccgaa
     9781 gccaaggaaa ggccacatgg tattttgccg acgagttcca tttgcttttg aaagaggaac
     9841 agacggcggc ctattctgcg gagatttgga agcggtttag gaaatggggc ggcattccaa
     9901 caggtgccac ccagaacgtc aaagacctgc tttcctcccc ggaaattgaa aatatccttg
     9961 aaaacagcga cttcattacg ctgctcaatc aggcgtccgg ggatagaaaa atccttgcgg
    10021 aacgcttgaa tttgtccacc gaacagcaga agtacatcga caattccgaa cccggcgagg
    10081 gccttttgat ctttgaaaac gtggtgctgc cctttaccaa ccccatcccg cacaacaccc
    10141 agctttacaa gattatgacg acccgcttaa acgaggtggc gggagtatga cccggcgacg
    10201 ccacaaagga ggtgaaccac catgaaagag ctgaaagcca aggcgaaagt gacgcaggcc
    10261 atgacccgcg acgggctgac gctggaaaat caggccaccg gcgaggtgga gaacatttcc
    10321 agccgggagg ccgaacagga tttgtccgcg ccggggccgg gcggcaccgc cgataagctg
    10381 ctggaacggg ccgacgccat ccatgacggc cataaggcca agaaagccgc gaagcaggcc
    10441 ggggctgtgg ccgccgaggg ggacgctgcc ctgcaccggc catccgcccg gctccaactg
    10501 tcggaggaag aacgggccga cccggattta cagccctata tccgaaaatc ggacaaacgg
    10561 gccgataagc tggataaggc ccgcgccgcc atccccaaga agcgcgtccc ggtcaaggaa
    10621 accgtctttg acgaggcaag cggcaaagca aagaccgtcc tgcactttga caaacaggac
    10681 aaggggcctc cctccctgaa acccaatccg gcaggccgtc ctgtttccga ggtttttcta
    10741 ttcggccacg ggaaaatcca tgaagtcgag catgaaaacg tgggtgtgga gggcggccat
    10801 aagggggagg aacttgccga gcggcaggcc ggaaaagcca tccgcagcac catccggcac
    10861 cacaggctga aaccctactg ggacgcggaa aaggccgagc gcaggctatt gtctgccaac
    10921 gcggagtatt tctaccagaa atctttgcgg gataacccgg agatcgcaca ggcggccagc
    10981 aaccccattt cccggttctg gcagaaacag cagatcaagc gccagtacgc caaggccgcc
    11041 cggacagcgg gccagaccgc aggcgcggcg gcacagggcg cggccaagac agccggggcc
    11101 tccacgaaag cggccaaggc cgcagccaaa gaaagtgaac gggcggcctc ctttgtggcc
    11161 cgccactgga aaggggcgct ggccgtcctt gtgttggggc ttttgatgct gcttatcatc
    11221 ggcggcttac agtcctgcac cgccatgttc ggcagcgccg gaagcggcat cacggcgtcc
    11281 tcttactttt ccgaggatag cgatatgttg gccgccgagg acgcctacgc ggagctggaa
    11341 gccgagctgc aagactatct ggacaactac gaggccaccc atgactatga cgaataccat
    11401 tttgaccttg acgagatcag ccatgacccc tatgtgctga tttccattct gtcggcgctc
    11461 catgacggga tatttaccat cggggaggta cagggtgatt tgtccatgct ctttgacaag
    11521 caatatatcc tgacagaaaa cgtcgtggta gaaacccgtt accgcaccga aaccgataca
    11581 tggacggacg cggagggcaa cacccataca gacacctacc aagtgccgta tgactattac
    11641 atctgctacg tcacgctgga aaacttcgac ctgtcccatg tgccagtcta tatcatggac
    11701 gaggaaacgc taagtttcta ttccctgtat atgtccacgc taggaaaccg gcctgatctg
    11761 ttttccggca gccagtaccc caacgcctcc accctgacgc cgccgaccta ctacgacgta
    11821 cccccggagg ctttggagga tgccaccttt gccgccatgc tcaaagaggc ggaaaaatat
    11881 atcggttatc cctatgtgtg gggcggcagc tctcccagca ccagctttga ttgcagcggc
    11941 ttcatttcgt gggtaatcaa tcatagtgga tggaacgtgg gacggctggg ggcgcagggc
    12001 ctatataaca tctgcacccc tgtttccagt tcgcaggcca agcccggcga ccttgtattt
    12061 ttcaagggaa cctacgacac ccccggcgtg tcccacgtcg gcctgtatgt gggcaacaat
    12121 gtgatgctgc attgcgggaa cccgatttcc tacacgaacc ttacttcaaa ctactggcaa
    12181 tcccattttt acagttacgg gcgtttacct taacgccaga aaggagcttt caattatggc
    12241 aaccaaactt gaccgcatcg aaaaggacat ccagaaaacc aagtctaaga tcgcggagtt
    12301 tcagaaacag cttcgggagc tggaaacgca gaaaaccgaa caggagaatt tgcagatcat
    12361 ccagcttgtg cgcggcatga acatgaagcc ggaggaattt gccgcgttct tacgcagcgg
    12421 cgcaatgcag gccgcccctg ccgcaacccc ttaccatgaa caggaggaca atgcccatga
    12481 agaataagac cagacggatt tcccgtattc tccccgcact tctggccgtg atgctctgcg
    12541 tcacggcctt tcccgttacc gcccttgcag gcggcaatga cccggcaccg gccccgctgc
    12601 cggaggctac aaccgaaccc gctacgggcg gtttggagcc tgaaacagac gaaaccggca
    12661 ccgccacgga cgggacagaa accgaggccg aaaaagatac cggccctctg accggcaagg
    12721 acatcaccga cctgttttcc gcgctgttcg gttccaaggt gagcattgcc gccacggacg
    12781 acggcatcca gatcaccacc ggcaccgaca aaaaagaacc tgaacagacc ggcaccgtca
    12841 ccaccaacgg cggcaggctg aatgtccgca ccggcgcggg gcttgacaag accgcgttca
    12901 cccagcttcc caatgggacg acggttgagg tagtcggcac agacggcgac tggattaaaa
    12961 ttctcttgcc ggagcgcatc ggctatgtcc attccgacta tatgaccgtc agcgaaaagg
    13021 aagtagcggc taccggcgag ggcggttttt ccctctccat tgacccggag gaaattgctt
    13081 ctctgctgga actgttcaat ggcggcgggg ccagcgccgc cctgtccccg gacgggaacc
    13141 tgtcactgat tgacgacatc ggcagcgccg cgaaaaatgg caagcagttt atcaccgtgg
    13201 aaaccaaaaa cggcaatgtg ttttatctga ttattgaccg ggacgacgag ggaaaagaaa
    13261 ctgtccattt cttaaatcag gtggatgaag ccgatctgct gacgctgatg gaggacgggg
    13321 aaaccgtcac ccccgctgct gtctgctcct gtaccacgaa atgcaaggcc ggggctgtga
    13381 acacgaattg cccggtatgt aaaaccaacc tgaccgagtg cagcggcccg gagccgcagg
    13441 aaccccagcc ggaggaaccc gaagccccgg aggaagaacc caagggcggc gcgggcggcc
    13501 tgatcgtgtt cctgctggtg gcgctggcgg gcggcggtgc ggccctttac tatttcaagt
    13561 tcaaaaagcc caaggctgaa accaccggca gcgacgattt gggcgagtat gattttgggg
    13621 aggacgagga tttggaggac gaggaacccg aaaccgagtt tgacccggaa gctgattttg
    13681 tgagtgaaca ggaggacgag gaatgacata taaacttgta atcgcggaaa aacccagcgt
    13741 ggcccgcagc attgcgggcg tgatcggggc cacggagaaa cacgacggct atttggaggg
    13801 gaacggctat ctggtgagct ggtgcatcgg acatctggtg tcctttgccg acgccggacg
    13861 ctatgacgag cgtttcaaaa aatggcgcta tgaggatttg cccattctcc cggaaacatg
    13921 gcagtacatc gtccccgacg acaagaaaca gcagtttgac gttctctgtt cgctgatgca
    13981 gcgccccgac gtgaccggcc ttgtgtgcgc caccgacgcc gggcgcgagg gggaattgat
    14041 tttccgtttc gtctaccaga tggcgggatg ccaaaagccc tttgagcgcc tttggatttc
    14101 ctctatggag gacagtgcta ttaaggacgg ctttgcccac ctgaaaccgg gcggcgacta
    14161 tgatccgctg tatcaatcgg ccctgtgccg ggccaaggcc gactggctga tcggcattaa
    14221 cgccacccgg cttttttccg tcctgtacca caagaacctg actgtgggcc gcgtccagac
    14281 gccgacctta aaaatgctgg tggacaggga cgccaagatt accgatttca agaaagagaa
    14341 atatcacatc gtccatatca cggcgggcgg cgcggacgcg gtaagcagcc gcttttccga
    14401 cgctgccgag gcaaacaccg tcaaggcggc ttgtgcaaaa gcgcaggccg tttgcgtttc
    14461 cgtgacgcgg gagaaaaaga cggaacagcc gcccaagctc tatgacctta ccaccttgca
    14521 gcgggaggct aaccggcttt tcggctatac cgccaagcag acccttgact atgcccaggc
    14581 actgtatcta aagaagctgc tgacctatcc gagaacggat agtcaatatc ttaccgacga
    14641 tatgctgccc gccgtggaaa gccttgtgtc cggcctgtgg gagctggtgc cgtttgccaa
    14701 gggcctgaac ctttccccgc agtttgaccg catcctgaac agcaaaaagg tgtccgacca
    14761 ccacgccatt atccccaccg ctgaatttgt gaagcagggc tttggcactc tggccgagag
    14821 tgaaaaaaag ctgctgtccc tgatctgctg caagctctta tgtgccgtgg ccgggccgca
    14881 tatctatgag gccgtcaccg ccacatttac ctgtgccggg caggagttta ccgccaaggg
    14941 caagaccgtc ctttcccccg gctggaaaga gattgaacgc cggttccgtt cttccttaaa
    15001 gaccgacgcc gacgaggacg ccgaggcggt gcgggagctg ccggagcttt gcgagggcca
    15061 gacctttgca gacgtggccg cctctgtcac cgagcatttc accacgccgc ccaagcccta
    15121 caccgaagat acgcttttgt ccgctatgga acgggccggg gccgaggata tgccggagga
    15181 tgccgagcgc aagggattag gcacaccggc cacccgcgcc gccattctgg aaaagctggt
    15241 gcagatgggc tttgtccaga gaaaaggcaa gcagctcatt cccaccaagg acggcatcaa
    15301 tctggctgtg gtgctgccgg aggcgcttac ttctccccag cttactgccg aatgggaaaa
    15361 ccggctgacc gagatcgcca aggggcaggc cgatcctgac gaatttatgg acggcatcga
    15421 gacgcaggcg cgggagctgg tgcagaccta ttcctgtatc agtgaggaaa agcagaaatt
    15481 gtttcaggcc gagcgtgttc ctatcgggac gtgtccccgc tgtggtgagg ctgtctatga
    15541 gggcaagaaa aactactact gcggaaaccg ggcctgccag ttcgtcatgt ggaagaatga
    15601 ccgatttttt gaggaacgca agaaagcgtt caccccgaag atcgccgctg cgctgctcaa
    15661 aaccggcaaa gtcaaggtaa agggcctcta ttccgtaaag accggcaaaa cctacgacgg
    15721 gacggtgctt ttggctgata ccgggggcaa gtacgtcaac taccgcattg aacacaggag
    15781 ctaaaggaca gtaacacagc aagcataggg agtgtggcta cactcccggt aaaatccccc
    15841 ggccgcgtcc ctgccgggcc ggggattttt gcttgttggg gaagtcccca accccctatt
    15901 ttttgaaaca gaacagagaa aggagtgacc gcgtgaacga agtattttct atccaagtcc
    15961 ataaccggca gtttttggaa cagggcaaac aaggcgtctg gctggatttg cccaccacaa
    16021 ccgaaacgct gcaagccgcc ctgcgggatg ttggcattac tgccgacaat ccgcaggatt
    16081 tttttatcaa cgggtattcc tgcccggagg acagacacct tgccattccc tatgacatgg
    16141 tgctggccgc cgacgtggac gagctgaatt tccttgccgc ccggctgggc cagcttgacg
    16201 ccgccgagat cgcagagctg aacgccgccc tgcaaaatcc ccgtggcggc tttgagaata
    16261 tcggccagat catcgactac accgaaaatg tggattacta tgtgcatctt cccgacgtta
    16321 ggggggatgg acagttgggc gactactacc tgaaccgttc cggcatggtg gatatgccgg
    16381 aggaatggaa agccgggatt gatacggcgc agtttggacg ccacattgcc cagcaggaac
    16441 aaggcgtttt tacccagtac ggctatctgg tgagaagcgg cgacgaatgg cagcgcgtcc
    16501 atgagggcca gccggtgccg gaggaatacc gggtgatgca ctacccccag ccggaagctg
    16561 accgggagga accgcaggcc cagcagccgg aggcatccga aagccccgac aaagcgccgc
    16621 ccccggttat cccgatcatg ctggacggca tgaacagcaa agaacgcatg aaagtgatta
    16681 ctgaccgtct ggaaaccggc gtacaggagc tttttgaaag tagccgctat atggcttact
    16741 tatccgctat gtccaagatg cacgattaca gtctgaacaa tacgctgctg atcgccatgc
    16801 agggcggttc gattgtggcg ggctataaca ggtggcagga tgaatttgac cgccatgtga
    16861 agcggggcga gaaaggcatt aagatactgg ccccggcccc gttcaaggtt aagcagcaga
    16921 tggagaaaat cgacccggcc acccagcagc ccgtgatcgg cgcggacggc aagcccgtga
    16981 ccgaagaaca ggaagtgacg gttccggcct ttaaggtggt gacagtcttt gacatcagcc
    17041 agaccgaggg caaggatttt cccatgccct atatcagtga acttaccggc gacgtgcagc
    17101 agtatcagga tttctttgcc gcgctggaac ggacatcacc ctatcccata agcattgagc
    17161 cgatttcaga cggggccaag ggccgctgct tctacgcaaa acagcgcatt gccatcaacg
    17221 agggcatgag cgaattgcag accctaaaga ccgccatcca tgagattgcc catgccaagc
    17281 tccacgccat tgaccgggag gcccccgccg aggaacaggc ggcccggcca gaccgcagca
    17341 cccgcgaggt acaggccgaa agcgtggcct ataccgtctg ccagtattac gggctggaca
    17401 cgtcggacta ttcttttggc tatgtggccg attggagcag cggcagggag ttatccgagc
    17461 tgaaagcctc ccttgaaacc atccgggcca cggccagcga attgataacc gagatcgacg
    17521 gccattttgc ccagcttcag caggagcgcg aggcccggca ggaacaggag gccgtcacgg
    17581 ttgacgttac cccggaggca gcggccccgg cgcaggccga ggaagaacag gccccggccc
    17641 ttgatccggc agccgagccg cttgtgactg tgctatggag cgaaagcccc cagctaaagg
    17701 acggccagca aatgccgttg catgaggccg acgcgctgtt tgcccgtctg gatgcagcat
    17761 atccggcaga ggcaggctat gacaaaaccg cgttccgcat tgactgtatc tttcagggcg
    17821 agccggacag ctacgagggg cggcaggatt ttggagatag ggacggttcc ctgatcgaac
    17881 atatccagag ctaccaccaa ttttacgcgc aggacgaaaa ctggaaaaag catgtaatcg
    17941 ctacaaccgg catagagggc tgggagcagg acaaggccca tcgggatatg ctgttaggcg
    18001 agtttgtccc ctatatgaag ctgcattgca gccttgccca aatggaacag acggcgcagg
    18061 gccgcttaca gtccggggaa accctgacgc cggaggaaac ggcctatttg ggggcgcttc
    18121 tctcttatgt gcaggagtgc cgccccatgc tcaatcaggg ccagtatcag ttgccggagc
    18181 cgccgaaact ggctgacttc gaccagagct tgcaggacta caaagcacag gtacaggccg
    18241 agatcgcgca ggaggccgcc gcagccggta tgaccgtgga ggaatacgcg gcggctggct
    18301 atgaagcacc ccaacaggac accttttcca tctaccagct tcgaggcggg gaggaaaccc
    18361 gcgactatcg gtttgagcca tacgagcgtt tacaggccgc agggctggcc gtggacagtg
    18421 ccaactatga ccttgtttat acggcaccgc tggataaaag cacgtctttg gaggatattt
    18481 accgcacatt caacatcgac cacccggcag attttagggg acacagcctt tccgtttccg
    18541 acgtggtggt gctgcaccag aatggacagg acaccgccca ctatgtagac agcttcggct
    18601 atatggaagt gccggagttt ttgcgggaaa atcccctgcg taccgccgaa ctgtctacgg
    18661 agcagaacga aaacatgatt gacggcatta tcaacaatac gccgcccgaa ccgcagccgg
    18721 aggaacccga acagctatct tttgaggatg tggcagttgc ttccggccag acacaggagg
    18781 aacccccgga ggctgcgccc ccgcaaacgg ctgtccgcta ttatcccatc aatgagggag
    18841 ccgcccgccg cgccaaggaa gccatgagct tttctgacta ccggcccggc agcgccacgg
    18901 cggaataccg gcactatgtc gatcaggccg tggaaattgc ggagcgccag aaaaagcgtg
    18961 tggagccaga gtatcacgaa aagatagaca gcctccttga cacctacgcc cgcaagctgg
    19021 ccgagaacat gaataagggc tatgagatta ccgcccgcgt cccctctgtc ctgattgccg
    19081 ggccgtccaa tttcccggta cgggcgaaag aaaagcagaa cgccgccagt gaccgcaaca
    19141 tggaggaatt tcagtacatt cagggcttgc ttgacaaaat ccgcagcacc ggcatgggtg
    19201 gtatcagcgc cgacgatccg caggccgttt ccaagctgga aaagaaactg gaaaagctgg
    19261 aagcgtcgca ggagttgatg aaggccgtca atgcctacta ccgcaagcat gggacgctgg
    19321 acggatgccc ccatctgacc gaaagaggca ttgaaaacct gaaagcggat atggcgtcgg
    19381 gctggcacta tgagaaaaaa ccgttccagt cctggcagct ctccaacaat aacgccgaga
    19441 tacgccggtt aaagggccgc attgaggaat tgacccgcca aaaggaggcg gcctatgtgg
    19501 gatgggagtt tgacggcggc actgtggaga tcaaccgggt ggccaaccgc ctgcaaatct
    19561 tctttgaggg gaagccggat gctgctgtac gcgacgagct gaaaagcaac ggtttccgct
    19621 ggtcgcccaa ggccggggca tggcagcggc agttaaacga caccacaatc cgcgtcgctg
    19681 accgtatcaa gtgcatccag cccctttccg gcgaaaagcc cagcgcctta cagcttgcgg
    19741 cccgcaggga gaaagaaaag ccctctatcc gggcgcagct caacgcggcc aagaccgaac
    19801 aggccaagca gccaccggcg cgggaaaaat caaagggatt ggaggtagag taaatgcaga
    19861 agtttgaaaa tgtggacgtt ctggccgcgc tggaacagat catgcggcag aacaccgcgt
    19921 tttatcaaag tgactttggc attgacaaag acattatcag gcgggatgcc gccagcgatc
    19981 aggccgcaga caagaccctg ctctggatgt cccggccctc cgggacgtac tgtttccggg
    20041 aacgtgatgt tttcctgaaa gacacccgac agtacaacac atggaaattt tacggggagc
    20101 agacccgtga caagatactg gcctacgcgg tggagctgac cggcacacag ggcggcacta
    20161 tccggggcaa tctctatgag ctggactatc cgcagcattt ccggcacgtc attgaaaccg
    20221 cccagccggt gagtgtgaac cggctctttt atgagcatgg cacccgtgac atcccggaga
    20281 gccagcgttt tgacggaagc cctgaccggg tattgggaaa ctttttgtac tatgaggccc
    20341 agccccatga cccggctgtg ctgcaggagg cgctacggac ggagcagcac agccgggggc
    20401 gggccgtccc cggcgatttc aaagtccacg tcaccgcttt gcatgacagc cggattgaaa
    20461 ccgaggcccg gcgtattgtg gacagcctga aagatctgtc cgcgcctaac agccccaata
    20521 aaacgcattt catggtggag atctccccgc actttgcggc gattgcttcc accaaggata
    20581 atgaccggct ctttgccatg ctgccctata aatccctgtg ttttaccggg atgaaaggca
    20641 ggcatggcgt gtatgccgtg attaaccggg acgagcgccg ggatgtgagt attcgcaggc
    20701 cccggccctc tatccgcaag cagctatccg acagcaaaca ggccgccagc ccaaaaaagg
    20761 cggcggccag aaccaaaaag aatgaattgg aggtataagg aatgatgcac tttaccgtgg
    20821 aggaagaaaa cctgatctgt atgtaccaca atgcagaccg gcgcagaacg ataaacaggc
    20881 tcaccgccgt catgccggtt atggatgggg atatgcgggc gcaggcccag cagacccttt
    20941 ccaagctgga acgcatgacg gatgccgact atgacggcca gaagttccac ttcacagacg
    21001 aataacgaac agcagacgcc gggcgcggcg gccatgtggt cgcttgcgtc cggcgtcttt
    21061 tctttttgcc tattcctacg catttagaac ccggtattgt cggggatagg atgaagtatt
    21121 cgcttgcccc tgcaaacatg cgggagaagc cttgcagcgg gcggttttca atcccttaaa
    21181 tgcgtaggtt gcagctataa ttagacccct gaaacagata tagccgcgca gcgggtgtat
    21241 ctgtttcagg ggtgtgcagg tgatagccgc aaagcggcgt aagagggtgc agccctcttg
    21301 ccgtctatct ctgttccagc gggaagctcg gcaatgcttc cagcagtttc agggaaatgc
    21361 gctgccgtat atcttcgtca atgactttct tctttttgcc gtttgcctgt ctgatctcca
    21421 ccgtggaaag ctcgttgatg tagtcagcat aatgggccaa cactttttcc gtggcccacc
    21481 tctcaccggc aacggcggct ttgatcgttt catagtccag cagcttttct tgtccgtcca
    21541 tcctgatacc cccgtcagac tgattttttc tatgatacca tattcggaag catggaacca
    21601 gagaaaacgg cgcttttctg ctggtaaatt ctacgcattt agaacagcac ttcggcgggg
    21661 ataggataaa acacccacat accccgctga ccatgccgga aaagccttga aatataaggg
    21721 cttttacagc tctaagtgcg taggtttcca gtatcttttc cgctggcgct cggcctcttt
    21781 gcggcggcgc atccgtttgg agcaggccgc gcagtatttg gcccggttag acttcggcgt
    21841 gaacgtggcc ccgcactcac agcagcgttt cacttcgtcc cggtctttgg tgatctcggc
    21901 atacagggcc gcatccagcg ggaggacagc ggccttaaac cagcggcaca gaagcgaata
    21961 ggaaatgctc tgtacgcaaa tacattcttc gccgtcgtcc agcaacaggc agttgccgtc
    22021 acaatagttg cagcactcat gcgtcagctt ccgggccttg cgatattgag ggtaattcat
    22081 tcgtggaata ctcatagctc ttgcgtccat tcctttcccg gcgtcatacg caaaatgccg
    22141 tccacattct gcttgatgat gccgtattcc tgcatctgct ttttcgcttc gccgtacttc
    22201 tcatacagcc gttccttttc ctcggccaat cgcttgtact cggctttcag ggcgtacaga
    22261 tcggggagct ttttcacacc ctgtcccttt agggcctttg ccgcagcttc atacaaaatc
    22321 aacgtgcttt catgctcacg ccggaatttt gccttatccg ctgcattgcg gtattccata
    22381 gccacgggcc agagctgctt gtaggtagaa atattcttta tcagcaccgc catatccgca
    22441 agccggtgtt ccatagcttt gagggtagcg gcggcctcgt cgctggcggc ggtgatctcg
    22501 gccactttgc tttccaaatc cgggtaggtg tctatcccat gttcagtcag gaaattcaaa
    22561 gtctttgcgg cctgcttcaa attgtggagc ttcgcccacc tctcatagcc tgccgactgc
    22621 tgggccttaa tgttgttttc cagatcaatc cgcaaagaaa tccccttgcg ctctttgggc
    22681 gctctggtga taacgcggcc tttgatacgc tctttgatgg cttcctcggt gtaggcttcc
    22741 cccaatgtct tgcagcgggt aaagcgttcc tgtccgggag cgcggaaaga aataaacttt
    22801 cccggcttga tctcatagcc ctgcgcctgc atgagccgca ggaaatcatc aaaatctttt
    22861 gcctgcggga tagcggcgtc aatggctgct ttgagcttcg ctttccagct cgtccctttc
    22921 cgctgggcgt cccactcggc atagctcttg cccttactct ggccgggctt caccacggac
    22981 aatccgtttt ccttgcacag cctgtcgctg gtgcgccgga tataggcata gctctgtttg
    23041 ttggaaatgt acttgcgctg atttaccata tccacggcac agaaaataat gtgattgtgg
    23101 acgtggccct tatcaatatg ggtagtcagc acatagggat atttcccttg cagcacttcc
    23161 tcggcaagct gcttcccgat ctcatgggcc tgttccggcg tggcttcgcc gggggcaaag
    23221 gattgtatca ggtggtggcc cagattattg cccttttggt atgcctgcgc cagcagcatt
    23281 tcaaattcaa tgtccgctgt ttcataagag caggcaaagg aggccaccag cagcttgccg
    23341 tcagttttgt ccgggttctc gatgtagtca agggcttttt tcagcgttga cttgatagga
    23401 tgtatctttg taactgccat atctgcgcca gcgccccctt tatatcctcg atgtcctgtg
    23461 cgtacaccgg ccccataccg ttcagcctct ttgcaatctg attgacgttg acgccgatct
    23521 tctgtatctc ggcggtctgc gcccggatgt cgctggtgtc catgttgacg atatagccgt
    23581 caatgagcat cttccgggcg taggctgaaa agttcttcgt atgcagcagc gccattttct
    23641 gttcaatcat agcccgttcc tccggcgtca cagggacgcg cagcacgatt tttcgctttc
    23701 tgtttgccat gcttcaccgt cctttccatt caggggtttg gggatttccc caacaagcaa
    23761 aacggacgcg gcccggcagg gacggggcag gccgtttttc cgggagtggc tacactccct
    23821 atgcttgcta cgttactgtt tctccactaa caagacgttt ccaaagcccc aaactacccg
    23881 caaaatagcc ctctggaaag aaaaaagtgc aaaaaaatag aagctacgct gtgcagctcc
    23941 cgctaaaagt aaaacagagg atgccagtta cacatcctcc gtttcattct tcttcggctt
    24001 ttcaattagt atggcgctgt caatcgcgcc ctcgatgatc ggcaggtatt cttcggggca
    24061 gagtttcagc ttgtggctga ccctttgccg cagttcgctt tcctcccgca tgatctccgg
    24121 gttaaagtag cgttccacag gaagcccgca ggctttaata agctgtatga caaccggcag
    24181 actgggaatg gccccgtcgt tttcgatgtt ggcaagatag cgccagtcaa tatttatttt
    24241 ctctgccatt gctttgcgcg aaacgcgctt tgcttgccgg gccgctttca catccgcgcc
    24301 gaaagtttca aagccgggac aatcttcaat attcgccata tcgcacctcc cacttacatt
    24361 gtatatttca tactctgccc gtggaatgtt ggtatatgca cctttcttaa agtttatatt
    24421 tcacattttg tttcttttat tcttcgagca aaagaattat aatgtaccct gtggaggtgt
    24481 actatggact acatgacgtt gaaagaggca ggcgagaaat ggggtgtgcc cccacgatgg
    24541 ataaactact attgtgctgc gggacgcatc cccggcgctg taaagatggc gacgatctgg
    24601 ctcattccca aaactgcgga aaagccgatt gacaggcgta caaagaaagg aaaggcggtt
    24661 actgaatagt ataatgaaac cggcgcattt tggatatggt tttgaagaga gagaaagcat
    24721 ggtattttgt cacggaaaaa ctataagatg atagatggca ggctgctcca gaccgataaa
    24781 aagtattctc atctgaaaca gaaagaaaag atttacacat ggatgtatga agaagcgaaa
    24841 ctttatcatg acagaaatgg aaaatgcccg aaacaaagac gaggaagctg ctgctgtatc
    24901 agccgtatat ggtagaattg aaaaggccgg catctggatt ctttatggtg aagtctccag
    24961 acattggata actgctggtc taatgcgcct ttgagtatgg ggtgattgat tttaataccg
    25021 gggaggtacg gctttttgag gaaaatattc tgggatttac atttggcgat tattacattt
    25081 cctgtaactg caaggacacg gatgatgaag atatgaggaa tatagacttg ctgattttct
    25141 actgtcttga acggtattag gactgaatct gctgcgcgga gtgcggcaag agcgaccgga
    25201 gggagtacac acaggccctg cctgtgtgaa aagccatacc gcaaggtgca ttcgcaaaaa
    25261 agaggccgcg cattttgctg aggcttgggg tgtccccaac aagcaaaacg agaacgccgc
    25321 aggcgggcaa gtggtggatc agaatatccg taaaggatat tctgatccat tgcttgccag
    25381 tattatcggg atgttttgac aaccgctgta atcgaaacat cccaaacatt aagaaggtgt
    25441 taattattat tatcttgatt taattcgttt aaaccaataa tatagataca tacaccatga
    25501 aaagaagcga gtatcccaat gacggatgat taaaatccga aaagaaatcg ctttgaaact
    25561 acagggaaac tacagactgt tatgttatct tcttaaaatg gagggatttt tatgtcgata
    25621 cgaattctac ttgtcgagga tgatgatcat atctgtaata cagtaagggc gtttctggct
    25681 gaggcaggat atcaggtgga tgcctgcaca gatggaaatg aggcatacac caagttttac
    25741 gaaaacactt atcaactggt tattcttgat attatgctgc ccggtatgaa cgggcatgaa
    25801 cttttgcgtg aatttcgtgc gaaaaatgat actcccattc tgatgatgac agccctgtcg
    25861 gatgacgaaa accaaatccg ggcgtttgat gcagaggcag acgactatgt aacaaagcca
    25921 ttcaagatgc agattttact aaagcgggtg gaagccctgc tgcggcgcag cggtgcgctg
    25981 gcaaaggaaa tccgtgtcgg caggctgaca cttctgccgg aggatttcac ggtactttgt
    26041 gacggtacag agctgcccct gacacgaaaa gaatttgaaa tccttttgct gctggtgcag
    26101 aacaaaggca gaaccttaac gcatgaaatc attttgtcac gtatatgggg atatgacttt
    26161 gagggtgatg gcagcacagt ccacactcat atcaaaaatt tgcgggccaa gctgccggaa
    26221 aatatcatca aaaccattcg cggtgtagga taccgattgg aggaatcata ataatggaaa
    26281 gaaaagggat tttcattaag gttttttcct atacgatcat tgtcctgcta ctgcttgtcg
    26341 gtgtaacagc aacactgttt gcacagcaat ttgtgtctta tttcagagtg atggaattac
    26401 agcaaacagt aaaatcctat cagccattgg tggaactgat tcagaatagc gataggcttg
    26461 atattcaaga ggtggcaggg ctgtttcact acaataacca atcctttgag ttttatattg
    26521 aagataaaga gggaagcgta ctctatgcca caccaaatgc caatacatca aatagtttta
    26581 gatccgactt tctttatgtg gtacatagag atgataatat ttcgattgtt gctcaaagca
    26641 aggcaggtgt gggattgctt tatcaagggc tgacaattcg gggaattgtt atgattgcga
    26701 taatggttgt attcagcctt ttatgcgcgt atatctttgc gcggcaaatg acaacgccga
    26761 tcaaagcctt agcggacagt gcgaataaaa tggcaaacct gaaagatgta ccgccgccgc
    26821 tggagcgaaa ggatgagctt ggcgcactgg ctcatgatat gcattccatg tatgtcaggc
    26881 tgaaagaaac catcgcaagg ctggaggatg aaatcgcaag ggaacatgag ttggaggaaa
    26941 cacagcgata tttctttgcg gcagcttctc atgagctaaa aacgcccatc gcggctacaa
    27001 gcgttctgtt ggagggaatg cttgaaaata tcggtgacta caaagatcat tctaagtatc
    27061 tgcgcgaatg catcaaaatg atggataggc agggcaaaat catttccgaa atactggagc
    27121 ttgtcagcct gaatgatggg agaatcgtac ccatagctga accgttggac atagggcgca
    27181 cggttgccga gttgctgccc gattttcaaa ccttggcaga ggcaaacaac cagcggttcg
    27241 tcacagatat tccagccggg caaattgtcc tgtccgatcc gaggctgctc caaaaggcac
    27301 tatccaatgt catattgaat gcagttcaga acacgccgca gggaggcgag gtacggatat
    27361 ggagtgagcc tggtgctgaa aaatgccgcc tttttgtttt gaacatgggc gttcacattg
    27421 atgatactgc gcttccaagg ctgttcaccc cattctatcg cattgatcag gcgcgaagca
    27481 gaaaaagtgg gcgaagcggt ttaggacttg ccatcgtaca aaaaacgctg gatgccatga
    27541 gcctccagta tgcgctggaa aacacctcgg atggcgtttt gttctggctg gatttaccgc
    27601 tcacatcaac attgtaaata tttaaaactt aaatgatttt gaccgacagg tataaccctg
    27661 ccggtctttt tttgctgcta caggaaaact acagactggc tacagggaaa gtacagatgc
    27721 gcttgccata ataacaatcg taccagccac aaatcgtagt tttattgcaa aggaggcatt
    27781 cgatcaaatg gaaaaaaaaa gcaactatca ttccaatgcg gatcatcaca aacggcatat
    27841 gaaacaatcc gtggaaaagc gggcttttct atgtgcgttc attatttcgt tcacagtctg
    27901 cacgctgttt ttggggtgga gactggcttc cgtattggag gcaacacaga taccgcccat
    27961 ccctgcaact catacaggca gcagcactga cgtagtggag aatttggagg aaaacgctct
    28021 tgccaccgcc aaagaacagg gagatgaaca ggaatggagc ctgattttag tgaacaggca
    28081 gaaccccatc cccgcacagt acgatgtgga gcttgagcaa ctatcaaatg gtgagcggat
    28141 agatattcgg atttctccct atcttcaaga tttgtttgat gccgcaagaa ctgatggagt
    28201 ttacccgatt gtcgcatccg gataccgaac aacagaaaaa cagcaagaaa ttatggatga
    28261 aaaaattgcc gaatataagg cgaaaggcta cacctctgca caggctaaag cggaagcaga
    28321 aacttgggtg gccgtgccgg gaacgagcga gcatcagctt ggtcttgctg tggatatcaa
    28381 tgcggacgga attcattcaa caggcaacga ggtttataga tggctggatg aaaacagcta
    28441 tcgttttggt tttattcgtc gctacccacc agacaagaca gagataaccg gtgtgagcaa
    28501 cgagccgtgg cattaccgat atgttggcat cgaagctgcc acagagatgt acaaccaagg
    28561 ggtttgcctt gaggaatatt taaaaccaga aaaatgagaa aaggatgtta tgttatggac
    28621 agaaaaagat tgacacagcg tttcccgttc ctgcttccaa tgagacgggc gcagagaaaa
    28681 atgtgctttt atgcgggaat gagatttgac ggctgtcgct atgcgcagac gataggggaa
    28741 aaatcgcttt cccatttgct ctttgaaacg gattgcgcat tatacaacca caataccgga
    28801 tttgacatga tataccagga aaacaaggtg ttcaacttaa agctggcagc aaagacctta
    28861 aacggcttat tgataagacc gggggaaacc ttttctttct ggtggctggt acgccatgcg
    28921 gacaaagata ccccctataa agacggcctt acggtgacca atggtaaact caccaccatg
    28981 tcgggcggcg gtatgtgcca gatgagcaat ttactatttt ggatgttcct gcatacgcca
    29041 ttgacaatta tccagcgcag aggtcacgaa gtaaaggagt ttccagagcc aaacagtgac
    29101 gagattaaag gggtggatgc aaccatttca gagggctgga ttgatttaaa agtgcgaaac
    29161 gataccgact gcacctaccc aatatgggtg accttagatg atgagaaaat catcggtcag
    29221 gtgtccgccg acaaagagcc ccaagcatta tacaaaatta caaatggcag tatccagtat
    29281 gtccgtgaaa gtggcgggat ttatgaatat gcccaggtta aacggatgca agttgcctta
    29341 ggtaccgggg aaataataga ttgcaagctg ctttatacaa acaaatgcaa aatctgttat
    29401 cccctcccgg aaagtgtgga tattcaggag gagaaccaat gagaaaaagt gtgggcatta
    29461 ctgtttttgg atgcgaacag gatgaggcaa atgctttccg cgctttatcg ccggattttc
    29521 atattatccc tacgctgatt agcgatgcga tatcggcaga caacgcaaaa ttggccgctg
    29581 gcaatcaatg cgttagcgta ggccataagt ccgaggtttc cgaggcgaca attcttgcgc
    29641 tgagaaaggt cggggtaaaa tacatttcta cccgcagcat cggctgcgat cacattgata
    29701 cgactgccgc cgagagaatg ggaatctcgg ttggcacggt tgcgtattcg ccggacagcg
    29761 ttgcggatta tgctttgatg ctgatgctga tggccatacg gggtgcaaaa cccaccatgc
    29821 acgccgtggc gcaacaagat ttcagattgg atcgtatccg ggggaaagaa ctgggggata
    29881 tgactgtggg agttattgga accggccata tcgggcaagc ggtcgtcaaa aggctgcggg
    29941 gatttggatg ccatgtgctg gcctatgata acagccgaaa aatggatgca gattatgtcc
    30001 agcttgatga gcttctaaaa aacagcgata ttgttacgct ccatgtgccg ctttgtgcgg
    30061 atacccgcca tctgatcggt cagaagcaaa ttggagagat gaagcaaggc gcatttttaa
    30121 tcaacaccgg gcgcggagcg cttgtcgata ccggggcgct ggtggaggcg ttggaaagcg
    30181 gaaagctggg tggtgcggca ctggatgtgt tggagggcga ggatcagttt gtttataccg
    30241 attgctcgca gaaagtgctt gaccatccct ttttgtcgca gcttttaagg atgccgaatg
    30301 tgatcatcac accccatacg gcgtactaca ctgagcgtgt gctgcaggat actacagaaa
    30361 aaacaatcag gaattgtctc aattttgaaa ggagtttaca gcatgaatag aataaaagtc
    30421 gcaatcatct tcggcggttg ctcggaggaa catgatgtgt cggtaaaatc cgcaatagaa
    30481 attgctgcga acattgatac ggaaaaattc gatccgcact acatcggaat tacaaaaaac
    30541 ggtgtatgga agctatgcaa gaagccatgt acggaatggg aagccgacag tctccccgcc
    30601 atactctccc cggataggaa aacgcatggg ctgcttgtca tgaaagaaag cgaatacgaa
    30661 acacggcgta ttgatgtggc tttcccggtt ttgcatggca aatgcgggga ggatggtgcg
    30721 atacaggggc tgtttgtatt gtctggtatc ccctatgtgg gctgtgatat tcaaagctcc
    30781 gcagcttgca tggacaaatc actggcctac attcttacaa aaaatgcggg catcgccgtt
    30841 cccgaatttc aaatgattga taaaggtgac aagccggagg cgggtgcgct tacctaccct
    30901 gtctttgtga agccggcacg gtcaggttcg tcctttggcg taaccaaagt aaacggtacg
    30961 gaagaactta acgctgcgat agaagcggca ggacaatatg atggaaaaat cttaattgag
    31021 caagcgattt cgggctgtga ggtcgggtgt gcggtcatgg ggaacgagga tgatttgatt
    31081 gtcggcgaag tggatcaaat ccggctgagc cacggtatct tccgcatcca tcaggaaaac
    31141 gagccggaaa aaggctcaga aaatgcgatg attacagttc ccgcagacat tccggtcgag
    31201 gaacgaaatc gggtgcagga aacggcaaag aaagtatatc gggtgcttgg atgcagaggg
    31261 cttgcccgtg ttgatctttt tttgcaggag gatggcggca tcgttctaaa tgaggtcaat
    31321 accatgcccg gttttacatc gtacagccgc tacccacgta tggtggccgc cgcaggaatc
    31381 acgcttcctg cactgattga cagcctgatt acattggcgt taaagaggtg ataagtatgg
    31441 aaaatggttt tttgttttta gatgaaatgt tgcatggcgt ccgttgggat gccaagtacg
    31501 ccacatggga taacttcaca ggaaaaccgg tggatgggta tgaggtgaat cgcatcatcg
    31561 gcacaaaggc cgtggcgttt gctctgcgcg aggcacaaat ccatgcggct gcgcttggct
    31621 atggcttgct tttatgggat ggatatcggc caagaactgc ggtggactgc ttcctgcgtt
    31681 gggcagcgca accggaggac aagctcacaa aagaaaaatt ttaccccaat atagagcgag
    31741 ccgagttgat tacaaagggt tatgtggcct cacaatccag ccatagccgt ggaagcgcaa
    31801 ttgatcttac gctctaccac ctggatacag gggaacttgt ttcaatggga agtaacttcg
    31861 attttatgga cgaacggtcg catcatacag caaaagggat aggggatgca gaggcacaaa
    31921 atcgaagatg cttgcgtaaa atcatggaaa gcagcggatt tcagtcttat cgctttgaat
    31981 ggtggcacta taagttgatt gatgagccat accccgatac ctattttaat tttgccgttt
    32041 cataatgaaa gtattggttt tgtatcagat tttggatttg ataaaatcta tgctggtcat
    32101 ggacaatctt ttactggaaa gtggttaaag catcttttgt aatacattgc aaattatagt
    32161 attcaatctg cccagcggca gaaaagaaaa aaaccgctgt tgggcagacc tttcccacgc
    32221 ccattttgta ttttgctggc ggcggttttg agaaatcaag gccgccgttc ttttctggca
    32281 gcaaaaggtg tggcggccca taggaggatg ccgctatgct gttttgctat tgccatgctc
    32341 taatccactt ccaccactca aaaaaagcct tgcccctcca cagggctatt ccggttatga
    32401 gtatgtacta taccatgtaa atcagcagca tagcaaagca ttttctcgcc cggtaggttt
    32461 gcgacctgcc gggcgatttt tgtcgtcttt tgcggcccgg ctgtccccaa aaaaattttt
    32521 tgagaaattt tcaaaaagga ggtatttagc gggtagctgg cggccttgtc aaagtcttgt
    32581 tagcggaaag ggagagaacc acctaagtcc cccacagaaa gggggtgaga agatggatgc
    32641 tatcccccgc gactatgaag cgcggtgcat gttcgatgct ttttgcaaga cggtactgcg
    32701 taacgaggcc aaaagctact tggctgaaat gaagcgccgc cgtgaccgtg aagtgtcact
    32761 aagctctttg tcacaggcag atttggacaa gctctgtaca gtagatcact accccagcga
    32821 cagctttacg ttttcgtcgc atggctatga cctacatatc aacaatgagc ttgttgccga
    32881 agcctttgcc gcgctgccca gcatggagca gagtatttta atcctgcact gtgttctgga
    32941 tatggcagat ggtgagatcg gcggccttgt gggaatgtcc cggagtgccg ttcagcggca
    33001 caggacaaat accttatcgg agctgcggaa acagctaaag gcgttgatgc cgaagggagg
    33061 ttagacgcgg atgaagaacc cgaactacaa gggcattgag cttttgcccc tttcggtgat
    33121 cgaggccgcc cgcgctgggg atgccggagc cgtagagcgc gttttgcagt attacagcgg
    33181 ttacatgaac aagctgtgta cccggacgct ctatgacgaa aacggctatc cgcatgtgtg
    33241 tctggacgag gccatgaagc gccgtctgga aatcaagctc atccacgcta tcgtcacgaa
    33301 ctgacgaacc ggccccggca gcatgaaacg gcttaccctt tccccgtttc ggtgttttcc
    33361 cggtggcctg atcgcacctt gataaatatg cccgcaagat aacggcggcg catcataggg
    33421 catacggaca cattctgtct gtgccgagcc ggacggctgg tgcgccatga tacttttccc
    33481 tacgggaaaa gcggagcgag gaacaccacg ccgcaatgtg agcgtggcgg cttgcgggcg
    33541 acgacgtaca ggcaggacta tgatactccc ttatagccac agtccgagcg ttaaaagcgt
    33601 cgcaggcaat gggtagggcc gcgtgagaac cacgcggggg tgaaagtccc gtggagctgt
    33661 gccagcagcc gtccgtctat gcccctaaat cacacaaacc tttgaatttg tgaaaggggg
    33721 ctgcattatg aaccatgatg tgcctatttg ggaaaaatac acgctgacga ttgaggaagc
    33781 gtctaaatat ttccgtatcg gagaaaacaa attgcgtaag ctggccgagg aaaatccggc
    33841 ctctggctgg gtcattatga atggcaaccg catccagatc aaacgcaagc agtttgagaa
    33901 aataattgac actttggaca caatctaata gtgaaaaagg gccgcagata tgttataata
    33961 atttataagc atatatgcgg ctctttccgt catggaaagg agcatgacga gatgtcagaa
    34021 aaaagacgag acagcaaaaa tcgcgttttg cggtctggag agagccagag aaaagacgga
    34081 cgatatgctt acaaatatgt tgataccttt ggaaaaccgc agtttgttta ctcatggaag
    34141 ttagtgccta cggataagac cccggcaggt aaacgggagg atatttcact ccgggaaaag
    34201 gagaaagaaa tccagaaaga ccttgacgac gggatagaca ccatcggcaa gaaaatgacc
    34261 gtctgccagc tctacgccaa gcagatcagg caccggggca atgtgaagca caacaccaaa
    34321 ctgggccggg aacggctgat gcgtattctg gaacaagatc ggctggggag ctgcccgatt
    34381 gacagtgtga agctgtccga cgcaaaggaa tgggcgcttc ggatgaaaga aaagggcctt
    34441 tcttataaga ccatcaataa cgacaagcgt tccctgaaag cggcttttta caccgccata
    34501 caggacgatt gcatccgaaa aaacccgttt gacttccagc ttagtgacgt tctggacgac
    34561 gatacggaac caaaggtgcc tcttacacca gcacaggaag aaagttttct gtcctttatt
    34621 caaggtgata aggtttacca aaagcattat gacgcaatcg ttattctgct ggggacaggg
    34681 cttcgcattt cggaactgtg cggactgacg gacaaagatt tggattttga aaaccgggta
    34741 atcatcgtga gccaccagct tttgagaaat accggcgtcg gttactatat tgacgaaccc
    34801 aagacccaaa gcggcgtcag aaaaatcccc atgaatgagg aagtgtatca ggcgttccag
    34861 cgagtgataa aaaaccgcaa aggtgcaaag ccgttcatta ttgacgggta tgccaatttc
    34921 ctgtttctca aacagaacgg ctatcccatg acggcggtag attatggcgg gatgtttggc
    34981 aggcttgtga agaagtacaa caagagccat gaagaagcgt tgccgaaaac gacgacgccc
    35041 catgctatgc gtcatacatt ctgcacccgg ttagccaatg cgggaatgaa ccccaaagcc
    35101 ttgcagtaca ttatgggaca ttccaatatc accatgacgc tgaactatta tgcacacgcc
    35161 acattcgatt ccgcaagggc agaaatggaa cggctggcag cctgacacaa ggccggtttt
    35221 actactccat ttactacttt tgagggggaa atggtgagag gtaataagaa catttgtaag
    35281 tatctaacaa tatcaaaaat gccggaaaag cccggaaaca cgggctatac cgacattcaa
    35341 gaacttctaa aaagataatc taaaatccca tataatttta ttttaaaata tgggatatta
    35401 tgataaatta actatggatg atatttgaaa aaaacatact gtatatttga tttaattttt
    35461 atttttaagc tcatatcgta agaatgggac ttgtagtctc acttctttat ggaggtgata
    35521 attatgaacg ttggttttat tggagccggc aaggtaggtt gttcattcgg gaagtatttc
    35581 caggaacata aaatacaagt aacaggattt tatagtaaaa gcgaagactc gtcattagca
    35641 gcttcgaatt ttacatctag taaacaatat ttaaacttaa gagaattagt agatgaaaat
    35701 gatactattt tcattacgac tcctgatggt caaattcagg aggtatggca aga
//
LOCUS       DAHHZJ010000010        35545 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.010, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000010 DAHHZJ010000000
VERSION     DAHHZJ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.010"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            48..929
                     /locus_tag="NBL02_000393"
     CDS             48..929
                     /locus_tag="NBL02_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2571107.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            942..2552
                     /locus_tag="NBL02_000394"
     CDS             942..2552
                     /locus_tag="NBL02_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HCD2571108.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2629..3288)
                     /locus_tag="NBL02_000395"
     CDS             complement(2629..3288)
                     /locus_tag="NBL02_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCD2571109.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3571..4236)
                     /locus_tag="NBL02_000396"
     CDS             complement(3571..4236)
                     /locus_tag="NBL02_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HCD2571110.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4383..4988)
                     /gene="dhaL"
                     /locus_tag="NBL02_000397"
     CDS             complement(4383..4988)
                     /gene="dhaL"
                     /locus_tag="NBL02_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HCD2571111.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(5016..6026)
                     /gene="dhaK"
                     /locus_tag="NBL02_000398"
     CDS             complement(5016..6026)
                     /gene="dhaK"
                     /locus_tag="NBL02_000398"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCD2571112.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(6001..6447)
                     /gene="dhaM"
                     /locus_tag="NBL02_000399"
     CDS             complement(6001..6447)
                     /gene="dhaM"
                     /locus_tag="NBL02_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HCD2571113.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6550..6870
                     /locus_tag="NBL02_000400"
     CDS             6550..6870
                     /locus_tag="NBL02_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCD2571114.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(6982..7851)
                     /locus_tag="NBL02_000401"
     CDS             complement(6982..7851)
                     /locus_tag="NBL02_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCD2571115.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(7947..9251)
                     /locus_tag="NBL02_000402"
     CDS             complement(7947..9251)
                     /locus_tag="NBL02_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCD2571116.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(9248..9757)
                     /locus_tag="NBL02_000403"
     CDS             complement(9248..9757)
                     /locus_tag="NBL02_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571117.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(9932..12067)
                     /locus_tag="NBL02_000404"
     CDS             complement(9932..12067)
                     /locus_tag="NBL02_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCD2571118.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12150..13805)
                     /locus_tag="NBL02_000405"
     CDS             complement(12150..13805)
                     /locus_tag="NBL02_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCD2571119.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13807..14121)
                     /locus_tag="NBL02_000406"
     CDS             complement(13807..14121)
                     /locus_tag="NBL02_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCD2571120.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14291..14551
                     /locus_tag="NBL02_000407"
     CDS             14291..14551
                     /locus_tag="NBL02_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HCD2571121.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14565..14879
                     /locus_tag="NBL02_000408"
     CDS             14565..14879
                     /locus_tag="NBL02_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HCD2571122.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15609..16163)
                     /locus_tag="NBL02_000409"
     CDS             complement(15609..16163)
                     /locus_tag="NBL02_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCD2571123.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16289..18218)
                     /locus_tag="NBL02_000410"
                     /pseudo
     CDS             complement(16289..18218)
                     /locus_tag="NBL02_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(18524..19240)
                     /locus_tag="NBL02_000411"
     CDS             complement(18524..19240)
                     /locus_tag="NBL02_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HCD2571124.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19241..21064)
                     /gene="glpO"
                     /locus_tag="NBL02_000412"
     CDS             complement(19241..21064)
                     /gene="glpO"
                     /locus_tag="NBL02_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HCD2571125.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21078..22574)
                     /gene="glpK"
                     /locus_tag="NBL02_000413"
     CDS             complement(21078..22574)
                     /gene="glpK"
                     /locus_tag="NBL02_000413"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCD2571126.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22710..24170)
                     /locus_tag="NBL02_000414"
     CDS             complement(22710..24170)
                     /locus_tag="NBL02_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD2571127.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24160..25455)
                     /locus_tag="NBL02_000415"
     CDS             complement(24160..25455)
                     /locus_tag="NBL02_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCD2571128.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25787..26464)
                     /locus_tag="NBL02_000416"
     CDS             complement(25787..26464)
                     /locus_tag="NBL02_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HCD2571129.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26494..27585)
                     /locus_tag="NBL02_000417"
     CDS             complement(26494..27585)
                     /locus_tag="NBL02_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HCD2571130.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(27686..28120)
                     /locus_tag="NBL02_000418"
     CDS             complement(27686..28120)
                     /locus_tag="NBL02_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCD2571131.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28704..30161)
                     /gene="ascB"
                     /locus_tag="NBL02_000419"
     CDS             complement(28704..30161)
                     /gene="ascB"
                     /locus_tag="NBL02_000419"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCD2571132.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30158..32053)
                     /locus_tag="NBL02_000420"
     CDS             complement(30158..32053)
                     /locus_tag="NBL02_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCD2571133.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32111..33886)
                     /locus_tag="NBL02_000421"
     CDS             complement(32111..33886)
                     /locus_tag="NBL02_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCD2571134.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(33936..35432)
                     /locus_tag="NBL02_000422"
     CDS             complement(33936..35432)
                     /locus_tag="NBL02_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCD2571135.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
BASE COUNT         9923 a         7574 c         6154 g        11894 t
ORIGIN      
        1 tttaatcttg tttagtatat atttttagaa aaaggagtgg ggtttttatg gcagaaatcg
       61 tcaaagtcac acatttgaaa aagagctttg gtcgtttcga agctttaaaa gatgtctctt
      121 tcaccgtaca ttcaggtgaa gtcgttggat ttatcggacc aaatggttct ggaaaatcca
      181 cgacgattcg gacactttta gggattataa aaaaagacgg cggaacggca acgatttttg
      241 ggcaagatgt atgggaagat agtttatcta tccatgaacg tctttcttat gttccaggcg
      301 atatctcttt gtggggaaat ttgactggag gagcgatcat cgatctcttc atgaagcttc
      361 atggaaaagg gagtgctcaa aaaaaagatg aactgattca acgtttctct cttgatccta
      421 agaaaaaagc aaaaaactat tcaaaaggaa atcgccaaaa agtgggcttg attgctgctt
      481 tggctgttga ttcggatctc tatttatttg atgagcctac ttctggttta gatcctttga
      541 tggaaacgat tttccaagaa gaagttgaaa aattgaaaga acagggcaaa tccatcctac
      601 tttcttctca tattctcagt gaagtcgagc gactagcgga taaagtcgtg atcatacgcg
      661 aaggaacact cgatgaagaa ggaacactcg atgaattgcg tcatttgact cgttcgacca
      721 tcactataga aacagaagca gagaatagtc acttagcaca attgaacggt gtccatgact
      781 ttgtgcaaaa aggtcacctc gctcaatttg ctgctgataa cgaagctttc gatgtgattt
      841 tagctgaagc gtccaaatta ggtgtgaaaa aatttgaatc tattccaccg actcttgagg
      901 atttattcat gagacattat gaaggatgag gaggaagcga aatgactgat aaattttcac
      961 gctggtccac ccttttttct caaaacctta gccgtgactg gaaaaagatc ctgatatggc
     1021 tagtcggtgt cactgctttt tctggtggtt ttgtccctgc gtttgaagaa ttgacttccg
     1081 gacaaggatt agctggatta tacgaaacct tgcaaaaccc ggcaatgatt gcaatggtcg
     1141 gaccaactcc agcttcgaat gctgctgagt atactttagg agcgatgtat gcaaatgaaa
     1201 tgttgctttt ttgcagtttg tttgctgctg tgatctctgt tttacacgtg gtgagccaaa
     1261 cgagaaaaaa agaagaactt ggtctgattg aacttattcg ttcctttcga gtcggacgtc
     1321 aagcaaactc tttagctgtc atcgctgaaa gtattctgat caatttgctt ttagcaatat
     1381 cgatcagttg tacgatgatt tttttccaag cggattcgat cactgcagaa ggtgcttggc
     1441 tttttgggct atctgtaggt agtatgggga ttttggggag tgccgttgca ctgtttgctg
     1501 cacaactgat gccaagttca gccagttcga atggtctgtc tttagcgttt ttaggtggac
     1561 tttaccttct acgagcaata acagatgtag ctgattcctc cctttcgatg tggaatcctt
     1621 taggctggtc ttatttgacc tatccattta caactaacaa ctggattcct ttgctttatt
     1681 tgtttttgtt cagtctaatc ttgataatca gttcatttgt tttagaggaa catcgagatt
     1741 tgaacaccgg ctatatcccc gaacgtgaag gaaaaccgta tgctaaacgt tctcttttat
     1801 ccttgcgtgg tttattattc tatctaaata aagggatgac aatcagttgg cttatcactt
     1861 ttggtttgat gggggcggca tacggctcta tctacgggaa tatgcaacag tttttagaaa
     1921 gtaacgactt aatgaaacag atgtttatcc aatctggcgt ctcaattgaa aaaaacttta
     1981 caagcacgat catggtcgtc ttgatttgtc tagctgctat tttaccaatt gctagcatca
     2041 atcgtttatt tacagaagaa acccacggac gtatgaatca gctgttcgca acaaaaattt
     2101 ctaggaacca actttattgg accaatatgt tactagctat tctatttgct atattggcaa
     2161 tcctgattag ttctggtgca ctagggcttt ctgctttggc ttcgatgaag gatgcgaaag
     2221 acatggatct attgatgttt cttaaagccg ggttcaattt cctgcctgcc gttttagtgt
     2281 ttatcggaat tagtggcttt ctattcggat ggcttcctcg aatcggtaaa cttgcttata
     2341 tctacttagc ttactccttt gcactcaatt actttggcgg aattttggat ttaccagaat
     2401 ggttttccaa aacagctgtc cctagctggg ttccacggat gccaaacgat tcttttgatc
     2461 tttccgtgtt ccttacgtta tcatgtatca gccttgcact gttcgtcctt ggttctattg
     2521 gttataggcg aagagactta ctagaaggat aataaaaaag catgtacaat ttcatgctag
     2581 cctccaaatc tgggtcaaaa atccaacatt tggaggtcat ttttttattt acaagtcttc
     2641 taactgctct ttatcaatga tccagacttc tcgattcttt ctttttacat aaccattctc
     2701 ctctaataat ttaaattttc tagataaagt ttcgggggtc gtaccgataa atgcagcaag
     2761 ttctttcatt ttcattggca ggaaaaagtg atccttccct gtcgctttag ctaagtcaag
     2821 cagatagaca gcgactcgtt cttctacttt ttccatcatc aaaaaagttg tctgctgctg
     2881 tacttttgtt aatttctcaa cgttgattgc taaaagtttc agactaaggg cgggataatg
     2941 cagtaagagt tccgagaaat ctttttgtct caatatgcaa atttccgttt tttctagtgc
     3001 ttcgccaaat aaagactcat ttggaacgcc tagcaactgg ctctctccct catatccgcc
     3061 cggctctatt actcgtaaaa gctgttcctt tccgctagca gataattgat acactttcat
     3121 actgccggag gccacgatca ctaattgagg ttctccattc ggcgcaagaa tctgttctcc
     3181 tttttccaca tagtgatgtt tgacaagtgc gtgtactttt tcttgatctt gctgtactaa
     3241 gtgattgaac aaaggaacaa atgaaacaca taaatgatca tgactcataa tctctcctcc
     3301 ttctaaacag ctatttctaa taaagaaaca taacttccct tattccaact gataattttc
     3361 ttttatcata gcataaacgt caagcgaacc agataccagc aaatagagtg gatttctttt
     3421 tgtcccttgc tttattttcc ttcttttgag accttaagta attgattcct atcaattttt
     3481 taaatattta ctttttgaat aatcaaaaac aaaccttcac tcgcaagccg agagtaaggt
     3541 ttgtttttcg cattgcttat ttcgtgttgt ctattgaaat ttttccgcac ctagcttttg
     3601 atatagtggt gctagttctt gtaaggtttc agcggttatt tgctcaacgt gttcgatttc
     3661 ttgcggatag aagaatagtt tacccatttg aaaggttcgg tctctcatcg cttttttcaa
     3721 ctcttcttgt ttctttgtat cggacaaata gtcagacaaa gctaagtggt cgtcaagttc
     3781 atgttctggt tgcggccata tgtaataatc cttgatatcg actgttttgc tccactcttt
     3841 cagtatcgca agcaactggt tatacgctgc aatgctacca tatcgttctt cgcttttata
     3901 atgttggtac atcaaataga tttggtattg tttttgattc aacaaaactg ctagacaggc
     3961 attctcgtct tgacgagatt cgcttcggta cgcgctccaa aaatgactgc gtaaattcca
     4021 gccattcgtc cagctttcga ttttaggttt gctcatgccg tacatatcag gcaaaagtgc
     4081 agctgtctgg agctgaatct ctttccattt ttgccagtgt tctttatatt ctttcttgat
     4141 ttgttcaatc ttatctgttg gatggctctc tttgagctgt ttgaatgcgt agattggtcg
     4201 atcaaacaac tgataatctg attttccaag ttccattgtt tcgcctcttt ccgttatctt
     4261 ttgtattcta cctgaatttt gcgatatgga caaatagagg ttgtgaaaaa gctgttctgc
     4321 atcaagtcat aagaacagcc aaacaaaaaa ctgcgatgag catcaccgca gttttattga
     4381 acttaaggtt gattgatcac atctaaaagc gttgaaaata aaatggcact agaagcagct
     4441 cctgggtcga tatgtccgat tgcacgctct cctagatagg aagctcgtcc ttttttagct
     4501 accaagactt tggtgtgttc ttttgcctgg tcaatgattt cttttgttaa attccctgcc
     4561 ttcaatgcct ctgtgactgg cagccaaaca tctaacatcg ttttatcgtc agcttctgcc
     4621 tttcctcggc tttgaatacc cgctgttccc tgctcgatca cttcgcctaa tttttcttga
     4681 gaatcaatag aatcgacctc ttttgtcgct ttcatcatgt tcatgaatgc agatccgtat
     4741 aacggaccag aagccccacc gactttcgat atcatcgcca tagagaatac tttgaatgta
     4801 tcgctgattg tttcaggatg atcggcttga aaagcttctt cataagctga aacccctctt
     4861 cccatgttat ttccatggtc tccatcgcca atcggggtat ctagatcact gagatattgt
     4921 ttgttctgat tgattgcctc tgcaaattgc gtgagccatt cttggatttc ttgaacggtt
     4981 agtttcatat tatcaccctt ttatcttgag attttttacc atgaaatcgt ctgtacattg
     5041 ctttctaaag aatctttcca ttgtgtctcc gtcaagtcga tcagtgttag agaaagacct
     5101 tgcatatcta gtgaggtcat aaaattaccg acttttcgga aaactacatt gacgcctctt
     5161 tcttccaaca gagctaaaac atcattcatg aacacgaact gttccatcaa aggcgtcccg
     5221 cccattccgt tgactagtac acctgcttct tttggtaaat tcgagtaatc atctaaaatc
     5281 ttagtaacaa gttcctttgc cagtacttta gaaggctgca tcttttctcg acgatagcct
     5341 ggctcaccgt ggataccaac accatactcg atctcatctt ctcctagttc gaatcctggt
     5401 ttccctactt ctggtacagt tgctgctcga agcgctagac cgatcgtctt agttgcctgt
     5461 acgacctttt ctcccagttc tttcaactca gctagggaag caccgtttct tgctgcatcg
     5521 cccacgattt tgtgtacaag gacagtacct gcaactccgc gttttccagc agtatacgta
     5581 ctattttcaa cggcgatatc atcatctacg atcacacttt ctacttcaat atcgtccata
     5641 gcagctaatt cctttgccat atcgaagttc aatgcatccc ctgtataatt cttaacgatc
     5701 agcaaaactc cttttccact atcggcttcc ttaatagccg tctgaatctg atctggagtt
     5761 ggagaagtaa acacatcacc aagcaccgct gcacttaaca tcccttcccc tacaaacccg
     5821 gcatgggatg gttcatgtcc gcttcctcca ccagaaacca aaccaacttg ttccgctttc
     5881 tgattcttgg ctacaacacg ggaagattct acacgatgaa caagttctgg atagcttttt
     5941 actaatcctt ccagcatttc ttcgacaata ttgcttggat cattcatgat ttttttcaat
     6001 ttattcccat cctttctttc cggcattttg tgcttctgtt aagatttgct gagtatcatc
     6061 tgttataccg gcagtgatgg ctgctgcaaa agctccttcc actaaaggcg catccaccag
     6121 atgataacgt tgctgctgct cttcttccaa catgtctttt gccagttccg agttcaatac
     6181 cgcgctaccg atatccgcaa aaacaaaata actagctgca tcgcttgatt cattgactgc
     6241 ttctacgatt ttcatcgggt ctgatccgat ctcgccattt attccgccga gagaaaaaat
     6301 ttgcacctct tcggattttt gcatctgctc aatcatttct ttaataccat ctgttacttt
     6361 ttgactatga gaaataagca aaatactttt tttcacaaga atcttccttt cttttacata
     6421 gttgctaacg attggtttcg ctttcattaa tgaacaattc aagtatataa taaatttcta
     6481 attttgggaa aatatgtgta gtgcttcttg aagtttgtta tactttaatt aaaagagagg
     6541 agtgatcgaa tgattacaac aacaacgaac agtgtagagg gaaaagccgt agtagcttat
     6601 aaagggatcg tttttggtga ggtcattact gggatccatg catttaaaga cttggaggcg
     6661 gggttgcgaa atatctttgg cggccgctca aaaagctatg aaaacgaact aatgggtgca
     6721 agagaagaag cacttgccga aatggccgaa cgtgcaaaag cattaggagc tgatgcgatc
     6781 attggcgtaa aaatggacta tgaagtatta ggcagcgaca atggcatgct gatggtcaca
     6841 tgtagtggaa cagctgtctc cacgagataa gtaggggaca tctgttttaa taactaaaga
     6901 gagatgggac atgtagtggc actcaagaat tagatttttc agtctaactt cgaggtactc
     6961 taccttccca tcctctcttt tttatttctt tttgcggatg attccgtaag ctgctgcgac
     7021 taacacaccc gcccctgcta aggaataact gacagaagct gttttttcat ttgtttttgg
     7081 caaagtctga cttgttgatt tggatggctc tgtcgtttgc tgaactgttt ctgtcgtgct
     7141 tccagaggtc actgattttt ctcctgccga atgcaaaata tcttttattc gttggaaact
     7201 agctgctaag tcagcttttt ttgcttcaaa atcattagca actgtttgtg cttcgctgat
     7261 ctttccgtgg attgcttgac tggccgctgt gatatccgct tgagtttgac ttagctttcc
     7321 agaagctgtt tgagcaacct gatttaattg ctgattgaat tcttttgctt gttggatctt
     7381 cccgtcaaga tctgtttgga cagcttccgc ttttgctttc gcttcggcaa gctttttctc
     7441 aagttgtgca cggtcatttc ccactttatt tttaagatca gctaaatctt gatcgattgc
     7501 tgcttggtct tctttcaatt tgttttttgc agcttctaat gcttcttcga gttcttttct
     7561 tttattttcc aagtcttgca aatgatcgat ttggtcagaa gaggtagaac tatttattga
     7621 tgtatcttct gaagatgaaa tcgtttctcc tgaattatct gtcggtacat tggaagtcat
     7681 ttctgtttcc gaggcaccgt tttcagcacc agaagagctt atggttgttg aaggttctgt
     7741 ttgagcaggt tcagaagttt ccgcaaaagc tgtctgtccc aataagaaaa tactggaaac
     7801 agcaattggc cagattaacg gcttcattct ttttttcatc acttttccca tccttactac
     7861 tttttaacta atttcttatt ataagcaaaa atgtttggaa gaaccataaa cacaacaggc
     7921 tgtttgtcta aaaaacggtg catcttttat ttttttgtac gttccgggct tttccatgtc
     7981 ccgcttccat aacctaaaat cgttttgact gctggaatga aagtcgtcac gatcgttatc
     8041 gcgttcacta tccagaacaa aagcatatat agtggtgcga aaagaaggta tttcaatttt
     8101 cttcgtcgat catctacaat cagcgaagct agcaactgga aaaatcctgc gaccatttca
     8161 aagcagatga acacaaaact gaatgtaaaa gtaatataaa tctgttcgta gtttccttga
     8221 tatacataat gcccgattaa tccagcaaaa agaactacgg aaatacaaaa gaatattgac
     8281 cagacaatac tcaatgtttg atcaatgaag atgatcgttc ttccaatatg tttaaaagga
     8341 tggagcatca cttttttgaa attcgtcagc cagacttctg ttccaccttt tgcccaacgt
     8401 tttctgtgtc gatatagcat cttcaacgtc acaggtactt ccatgaaaaa gataatctca
     8461 ggagcaaaaa cagataacca gtcatttaac tgatgatccc aagcaatact gatgtcttct
     8521 gtcgctcgat cttgtctgaa tccgcccaca tcgatcaagg cttcttttcg atacatcgta
     8581 tttgctccac tgtacgcata aagaccaccg aaaacagctg actgtgttcg tttaatgatg
     8641 cccacgatac ttgagaattc aaccgtttga gattttgcaa tcaatttggt acgattttgg
     8701 acatccatat ttgcagtgac cgcagcaata tgtcttgcac cagggcgaat gaaataattc
     8761 acatacctaa tcagtgcatc tggctccggt accgtgtctg catcattgct taaaatcaat
     8821 ttcccttttg caaaggcaag tccgatatta aacgcgtggg cttttccttt atttttttcg
     8881 atccgaacca cacgaagatt agcgtatttt ttcatcagtc tagctaatat ttctggtgtt
     8941 tggtccgttg aaccatcgtc tgtcactaat acctcatagt tgtgataatt caatttcgtc
     9001 atcaaatact cgattgtata ttcgatcaca atttcttcat tatgggctgg caccatgatc
     9061 gtgatgaacg gttcgacact caagggaaca tctacccatt ctttttgctt atgtttgtat
     9121 aaaaacacat aacaccagac accaataaac catacaaatc cgcctaaaat cggataagaa
     9181 acgagaaaaa aatacataat agacaaacaa gtatctaaga ataaattacc tgtatgggtg
     9241 attgagatca tttcagttcc accccatttt ctttatacaa atctgcaatg aatgtcgtat
     9301 ctagattttt ctcttcggag acagaataaa aacggaccgt ttctctctct tcttttgttc
     9361 caaaacgttc cgtatacact tcttcaagta attcttgtct ttttttcaat ttttcatctt
     9421 catattcaaa agatttattt acttttttgt atagatttcg attgttccac ctagtcaata
     9481 caaatgaggt aatcaggaaa ctgaaaaaaa tcaatacaaa aaagccgatt aagaaataca
     9541 gcattttctt ttctgataaa aaagcgtaca aggaaaaatc cttggctctt tctggtctcg
     9601 ttaatgggaa aaagacccat ataaaaggaa gaacaacgat catccatcct aaaagagcca
     9661 ctaacgtttg ccaaatcttt aaccagtaat taccttttga aaaatactta tctttcacca
     9721 aaattgatgc aacttcttct cttgttactt ttttcatatc ctcacctcac gtttttttgt
     9781 tacgacgtag gagtttacca tacatttcat ttcttttcac ataaagtgtt cctaaatttt
     9841 tcctaagaaa aagaacaaaa catttctcat cagcaaatga aaaatatttt gttcttttta
     9901 tcagttgttc tttttatttt ttatctctta attatgttgc tctttccaat acgacaatgc
     9961 ttttcattcc tctagatatc gccgtgtaca tttgtctgcg tcttctttgc tcatctccat
    10021 cgttttcaaa tggatataag attacatgat caaattccag tccttttgct agactcaaag
    10081 gaatgatttg gcagttttct aatccatatg aatttttttt aatcgattct tgtagcgttt
    10141 gggtttcctc ttctgtttgg gtgataactg ccgctttttc tccttttaga gaaaccaaaa
    10201 tctcagctag tattttcaac aattcttcag aggatcgaaa agagcggtat tcgggttctt
    10261 ttccttgcgg tcggatcgat acgatgtcga tcttctcttg atccacagca taggttttga
    10321 aaagctctgt gattgctccg ctagaacggt aactatttct aagatcatac cgatggatcg
    10381 gcagatggta gtcgtcgaaa caacgcataa tatcagcaaa agtagtggaa gaattaaaga
    10441 ttgcctgatt ctcgtcacct actaaagtaa agtgagtttt tggaaataat tcgatcaata
    10501 gacctaattg tgccagggta taatcttgta cttcatcaat caatagatag cgaaatgctg
    10561 gtacatttac cttttcaaca agtaaatgac gaatagctaa tataatgact gcttcatcaa
    10621 cagtacgtgt gggttgccaa gcataagctg agccatatcg tttttcataa attctttcaa
    10681 acaactgatg ctcatccacc catgccattt cttcgatttg tctggttatt tttcgatatt
    10741 ttttttgtaa aagtttcctt gcataagcta caatactttg ttccgaatca tcagaaatca
    10801 atttaccgaa taattccaac tgcctatctt ctgtcaacga gagtacctga ttctgcacag
    10861 caggactttt tgcttgtttc aaaagatgat tcttccattc gctcatcagt gcctttttcg
    10921 ttgcttgaag cctatcgatc aatttaggat gagcaggaac tttctcatat attttttcga
    10981 tcgttttttt agaaaggact gtttttccat ttttagtaag tccacggata aaattcggat
    11041 gatctaaaaa caaaggatct gattgtttta gattatcgat aaactttttg ctacgcaaac
    11101 gttctgtctg ctccgataca ttttctcccg tgatacgttt gaaataagcc tcttcacctt
    11161 ctatttcctc tgctgaaagc tgtgaaacca gctgtatgat ggttagattc agtggatttc
    11221 tttcacctaa tgatggtagg acattcgaaa tatagtcgat aaaccgatga ttaggtgata
    11281 gaatcagcaa gtcatcggat gtcatcgtat ctcgatataa atagagcaga tacgcaatac
    11341 gctgcatgat agcagaggtt ttaccacttc ctgctacgcc atttaccaag atattttgac
    11401 ttttcacatc ccgaatgatt gtgttttgtt ccgattggat cgtagcagtg atagccttca
    11461 tttcattggt ctcatcttgg cctaagactt ctaataatac gtcatcttga atagctacag
    11521 acgtatcaaa aaacttgacc aaacgatctc tttccaaaag aaattgtcgt ctttctttaa
    11581 taaccgtatc gattttattt tgacgaaccg tataagaaga tggaccaatc atgttattgt
    11641 aaaaaagttc ggcaattggt gaacgccaat cataaacaag attttcatct gcatcatctg
    11701 tgaaatttgc tgtaccgata taaaaggatt cttttggctc attatccaga aagtctacaa
    11761 cgacttttcc aaagtaggga cttttcaata aacgttcgat ctttgctagc tgattttctg
    11821 cgtttttctg tttgatattc atctgatcga tttctctatt tttcatttca atcatcgaaa
    11881 aagtatccag gttatctaag taactgtcaa aattcaatcg aagctcgcca gccatttctt
    11941 tcaacgactg gtttccttct atttttgccg tatccatcga ttcccgcagt tcgttttttt
    12001 tatcaattag cttttgatac acgttcttca gatatgcttc ttctaactgt ttttgtgtgt
    12061 tgaccataca cattctcctc tttttttaaa gtggtggaac cgattagttt acctctttta
    12121 agagtcgaat gcaagctttt taacgagtat catttgttag gatatatcag ttcttcaggt
    12181 cggacagttt gatataaaga aaatgcacca tcaaggtttt ttacgtggta gccagactgc
    12241 ttcaagattc tttccgctag atagctacgt aatccgctat gacagctgac gatatattct
    12301 tgttggctgt ctaattcatt taatcggtcc cgaagttcat tcaaagggat atgtttggct
    12361 tccttgaatt tgcccttttc caattcttct gctgttcgca catctaataa aatttttcct
    12421 tttgctagtt ctgtcggcaa ttcatgccat tgtacggttt cactaagccc ttctgcaatg
    12481 ttcattgctg catatcctag catgtttact ggatctttgg ctgagccaaa tggcggtgca
    12541 tacgtcaatt ctaattccgg taaatcgaag atcgtcaaat gccctttgat tgctgttgct
    12601 aaaatatcga tccgtttatc cacacctttt gccccaacac cttgtgcacc ataaatctcg
    12661 ccagtctctg gatggaagat caacttcagt aaaatatctg ttgcttctgg ataatagctt
    12721 gcatgatctt ttcctgatac atgaatgact tcaaaagaca gttcgttttc tttggctgtt
    12781 ctttcactta aaccggtgga tgcggcagtc attccaaacg ttcgtataat tgctgttcca
    12841 ataccgcctt gatttcttcg agaaattccg gaaatcgtgt ccgcaacttg tctaccttga
    12901 cgattggctg gtgaagcaag agaaataagt gcatcttttc cagtgatttg ctgttttaca
    12961 acgatagcat cccccaccgc ataaatatca ggaatattcg tttgataacg ttcgtctact
    13021 aggatacccc cgcgtatgcc taattcgatt ccagcggtca ctgctagctg gttatcagga
    13081 cgaacaccaa tggataaaat aactaaatca gcagggatgg tacgcccatc atccagtcgc
    13141 aatcgtttcc cgtggtcttc aaatccagcg acagcatgag aagtaatcac ttctactcct
    13201 tgatgagaca attctttttc tataaaagct gccatttctt catccagtgt tggaagaata
    13261 tgtggtgctt gttcgatcac catgacttct aaacctcttc tttttaggtt ttctgccatt
    13321 tccaatccga tgaatcctgc accaacgatc acggcacgct ttggctgttt ttctaatgca
    13381 tgcatcactt tatcgatatc tggtacattt ctaatagaaa aaacattgtc ggcttctgtt
    13441 atccctggaa tcgatggaac aactggtttt gcaccaggag ataaaaccaa tgcatcgtac
    13501 tgttcaatgt gtttttgacc ctcatgtacg acttctaccg ttttgttttc gggaaagatg
    13561 gctgtcactt catgatttgg acgcacatct aaacaaaaac gagcggataa tgattctggc
    13621 gtttggacaa gaagattttc ccgttcactg atttctcccg aaagataata aggcaagccg
    13681 caatttgcaa aagaaacata cggtcctttt tcaaaaacaa cgatttctgc atcttccatt
    13741 aatcggcgaa gccgtgtagc agcagacatt ccacctgcta cacctccgac aataacgatt
    13801 ttcatcttat ttccctccta ctgttcgatc aaaccattga ttcatgccac cacgtacatt
    13861 cactacatca taaccagatt tctttaattc tttagctgct tgtttactcc tcatccctga
    13921 ttgacaaatc acatagacgg tcttttcttt gtctccatca tatctgttta tactttgtaa
    13981 tggaacattt ttagctcctt ttatatgccc gccccggtat tctgcaggtg ttctaacatc
    14041 aagtaacgct ggattttcca aaagcttttc ctttaattct gggactgtta tcgattttat
    14101 tgattgaaat agcaaattca ttttctgcac ctcatttaca tttttataaa tacctcatgg
    14161 ggtatattaa acaggagctt tattaatatg tcaaccaaaa tgactcttca tttttttcac
    14221 tactttttat atttgcataa aataccgata aggggtatta tctaattata aaaatagagg
    14281 agaatgtgtt atggagattg ataataaaaa tatcctgaac cgtttgaaac gtacagaggg
    14341 acaaattcgt ggtatccaaa aaatgatcga agacgaaaaa gaatgtatgg atgtcatcac
    14401 tcaattaagc gctgttcgtt ctagtattga tcgcgtaatg ggcatgatcg ttgcagataa
    14461 tctgaaaaat tgctttgaga aacctgatag taatccagaa gaacaagcgg ctaaactaga
    14521 acaagcgatc aagatgatta ttaaaaagta aaggaatgat ttcaatgaaa gtcgttttcc
    14581 atattgatga attagaaaaa tggtcagaaa cagggaaaaa cgtaaaaaac ctgataaaag
    14641 cttctccaga aacgacgatt gtcgtatctg taaatggcat cgcgattacc ggttatctag
    14701 actctgccaa tgcagaattc ttagatttgc aaggagtcgt ttttcacgct tgcgccaatg
    14761 ctatgcgtgc taatcatatt tcagaaagtt ctttacctga acaagtaatc gttgtaccag
    14821 caggtgtcct tgatttagtt gaactgcaat cccaaggtta tgcttatatc aagccttagt
    14881 cagtagaggt tgcgacagaa gtgatcagct tcaagaaatt tcggcttatt tttcgaagaa
    14941 gctacttctg gaaaaccgtg aatcaaaggt tgtgaaaacg ctgtcctgca tcaagtaata
    15001 agaacagcca aacaaaaata gcttctcata tttttcgttt ggttgggctt attaccgcag
    15061 atgcaagctt ttgaacaccg tttaaggaag aggttgtgaa atcagcggtt tgacctaagt
    15121 attagagaaa aaaacgggaa aatagctcct catattttgc cgttttttga gctaataccg
    15181 aaggtcagct gattgaacac cgtttaagga agagattgtg aaaaagtcgc ttagtttcga
    15241 gtattaagaa aaattttgtg aaaatggatt tctacatttt tgcataaatt tggcttaata
    15301 ccgagaaact ggcttttgaa taccgtttaa ggaagaggtt gtaaaatcag cggtttgacc
    15361 tgagtattag agaaacaaac ggaaaaatag ctgctcatat tttgccgttt tttgagctaa
    15421 taccgaaggt cagctgattg aacaccgttt accaagaggt tgtgagccta ccatttaact
    15481 ccgaatcaca agaaataatt tcaaaaacaa cttctcctgt ttttggttcg gttggcttat
    15541 tatcgagaag tagctactgg gagtctattt atcaaaaaaa ggaatgcgac ataggtcaca
    15601 cccctttttt attttctttg aaacatcttt cggtactcga cagcatacaa gacaaaagat
    15661 tgtaaaagac cgtaagtgat gacggcaaaa atgactctca cccacagcgt tggtaattct
    15721 gggaaaagca gcaaaaggat catgccaaca tagatgacag cagtcgctac tttgccatac
    15781 cacttcgctc cacttaattt gatattttta gatagcccga ttaatccagc tacaaacata
    15841 aagccttctt ttatcaagaa tagaccaaac aacagccaaa gccacggtcg ataccatgcc
    15901 atagacaaaa tcagaaaaag ttgtgtcaat ttatctgcaa acggatcaag caccttccct
    15961 aagtctgtta cctgattata tctccgagca atgaccccgt ctagataatc agacaaacct
    16021 gagacaacaa tgaccgctcc tgctatataa aatgactgtc tcagcacaaa ataaagataa
    16081 atcggcagca aaaaaatacg gaatatcgat aaaagattcg gaatcgtttg tatttcttta
    16141 cgccagtctg tgcttaattt cattttatcg ccctacctcc tcagcactct tttcttttta
    16201 ttttagctta cacggggaat aaaaccaatg atttcactgt ttttattttt ctttacagac
    16261 actgatatta tgagctaatt tgagataatc acttgctgtt ttcatcgttt actcttcttt
    16321 cttcctgtcg tttgcgcatt tcttttacta gtactttatg agcttttttt gatcctcgct
    16381 ctaggtcacg ctgccgttta cgtgttttat aatcgataaa cagactgtct aaatcgtaac
    16441 ctgtcgggta cagatcttct gctgaacgca ccagacgaac acgctgacga ggcacgtgga
    16501 ttattttttt gtctaaatat acatcaatcg tatcttcacc tatatcttca taaactaacg
    16561 cttctttttg gtattcattg acaaatatcc gatcaccttt tgcaaagagc tgacgttctt
    16621 ttttattctc cttcctattt gtaactgctg tcttaggaag aaattctttt ttctgtgtcc
    16681 gataggcttt ttccgccaag taacgctctg cttgatggat caatttcatc gacatatcca
    16741 ttttatgagc aatccacaaa gcttggcttt ctcctgtttc ttccaacccg caactgatat
    16801 ttaggacgaa gtgcttcccg atcgaaagcc atagcagccg gcacaaaatc ttcatgatct
    16861 ccggcgaatt tcttgatttc tccataatgg gtcgtggcaa tgatcaatgc cccttgctca
    16921 tacatagatt ccatgattgc gatcgctagt gcagcccctt catttggctc tgttccactg
    16981 ccaatctcat ctaaaagtac caacgtattc ctttttatct ttttcaaaat agcagcgatg
    17041 ttttgcatat gaccagaaaa tgtactcaac gcattttcta aattttggtg gtcaccgaca
    17101 tcgacgaaaa tctcatcaaa gacagcaagc tctgtccctt cttttgccgg aacttgcata
    17161 ccgaacatcg ccatcaaagt caataaaccg actgtcttca acacgactgt tttgcctccg
    17221 gcatttgctc cggtgataat caagcctcga tagtctttac cgatttcaac atccaatggt
    17281 accgcatgct caggtaaaaa tggatgtctg ccttgcttga taatgatatg ttccgattta
    17341 ttgattctag gtgtgcttcc attgatttct cggctaaatt ttgcccgagc aaagatgata
    17401 tctaacaccg tgatcgtttc gatcaataaa tcgatcactt cttcgttttc tgcgatagca
    17461 ccagtcaatg ccgccaggac ttgatattct tcactgattt cttcagcttt taacaactga
    17521 tagtgttcat tcaatttaga aatcacagtt ggttcaataa aaaccgtcgt tcttttattt
    17581 gattcatcga taatcgttcc atctatttta tttttatagc tggctttgat aggaatcgta
    17641 tagtattcgc ctttttgaac aatcattgct tcttggatca tttctttgtt tttcggatga
    17701 cgcaagaatt tcagtaattt tgactggatt tctttttcga ttagttgtaa ttgtttgcgt
    17761 actttacgca aattacgtga cgcgtcgtca ctgactttat tattttttat tttctggtca
    17821 atcaactctt ctacatttat caaatcagga agatgtttgc tataagcaaa caacaagggt
    17881 gtctggtatt gattcttatc aaaaaacttt gtgatcatcc gactgcttct aagaaaatcc
    17941 gcatattcga tcaaatcggt tggctgcaga atcaaccctt ttttgacttg ttctgtcagt
    18001 gcatcgatcc tcggcaaccc cataaatgga acatgctgac tgctttctaa gatcaagcgt
    18061 gcttctcttg tttcttcttg acgatccaag acagtttgta ggtttgtcga gaccgtcata
    18121 tcgctgattc gttttttgct atagtttcca attgctcttg cttggatttc ctttttgatc
    18181 cgatcaaatt gtaattgact aatcatttcg ttgtccatat tctctccttt tcttgaagag
    18241 atacctgtcc caaaataaat aagggcctag agataaataa aaggatagaa cacagcttaa
    18301 aaaagtcatg tcctatcctt attagaatac aaagatattg ttttcctgac aggtaccgtt
    18361 tcataagcac accaaataaa ctttgtttat tggctattcg acttactgat tagataccag
    18421 ctacactcac aaaacatacc ccattcttct aggtcttatt tattttccac ttaactatag
    18481 caagacacat tttggttgtc aaactttagt atgttttata gatttacacc attaatgaat
    18541 agacacctac cgcgataatt gctccgatca ttggacctgc gattggtacc catgaatatg
    18601 cccaatctga agttccttta ttagctattg gcaataccgc atgtgctaat cgaggaccaa
    18661 agtcccgagc agggttgatt gcatatcctg ttggccctcc taatgaaaga ccgatggcta
    18721 agatcaatcc acctacagcg aatacgttcg ttccaggtgc aaattcgttt tgtccaaaag
    18781 ctagcaaccc aagaactaat acaaatgtac caattaattc tgtgatcaca tttgctggat
    18841 aattccgtat agcaggacct gttgcaaacg ttcctaaaat cgcgctttca tctttcgttg
    18901 cattccaatg tggcaaataa gaaagccaaa caagaatggc ccctgcaaaa gctcctaaaa
    18961 cttgcgcaat gatgaaagga acgacaagat tccaactaat tgcccctgtc atcgccatag
    19021 ctagagagac tgcaggattc aaatgagctg ggcccatgaa tccagaaata tagacagcca
    19081 ttgttacagc gagtccccaa ccaaatgcga tgacaatcca gccagaagcc tgtgctttac
    19141 ttttgtttaa attcacagcc gcacacacac catctccaag caaaactagg atcagtgtcc
    19201 caataaactc ccccatgatt tgagtcatat cactgttcat ttatttctcc ccctttagtt
    19261 cccgcagatc tgattctgca atcaatgctt ctaaacgttt agtctgttgc gccttctcct
    19321 cttctgtcca ttcaaaatat ccagcaattg catcgatcac aggttgcttg atctcatcaa
    19381 gctgatctcg ttcaaacaac agatgatttg tacgacgaat gagataatcg cctggggcca
    19441 aaaccatttc ttcttctaaa ccataacgta accgagctga ctcagcgaga ctcaaaccag
    19501 gataaggtgc catttctttt gctaattcaa agatacgtcg ggcattagtc ccgtaaaaat
    19561 cagcgatata agtagcatct tcttcctcta aaccggctgc tactccttct tttgctaatt
    19621 ctgtcactgt ttcttctaat ttagttggat cgaagtttcc acctgaaatc tgatattttt
    19681 tagagtcgat ttctttcgtc tctattccgt attcttcttt taataattgg cgtatcaatc
    19741 gcaaagctcc ttctgccatc ttacggtaat cggtgatttt cccaccagat aaagtcagca
    19801 aaccatctgg ttctctttct aaagaactac ctctggaaat cgtagaaggt gctttttcat
    19861 cacggctgtt ttccaaatgg ttcaacacgt cttctacttc agcacgagaa actttgtttt
    19921 ccttatattc acttactgta tcgacaactt tattgaagct cttgtctgaa atcgatccat
    19981 tatctccacc attgtaatca gaaccagaat taccaatcaa cagtggacga agtcctgccc
    20041 agcttgcttc gatatcattc aatgtgatat ttgcttctgg atagcgatga ttgatcacat
    20101 ccaatagata atctacatct tcttgtgtga ctttcggatc cgtaaaatct ccttggtaat
    20161 ccgtatctgt cgtaccaaag taagtcttgt tttctcttgg aatagcaaaa accatccgcc
    20221 catcttgctt tcctgtatcg aagtatgtgg gttgcggtac aggcagtttt ttcgcatcta
    20281 caactaaatg gatcccttta gttggacgca ttttaggaga gactggacgc gtaaaattca
    20341 agtttcgtac tttatctacc caaggaccac tcgtattgat cactaatttt gctttgattt
    20401 cgatcacttc atctgtcagc aggtcacgag ctttgacgcc aacgatttga tcgccatcat
    20461 ataaaaagcc aaccgctttc attttactta ctagataagc cccatcttcg gcagcctttt
    20521 tgatattatc aatcactaaa cgggcatcat tgttgcggaa atctagatag acacccgcgc
    20581 cttttagccc ttcttttttc aaaaacggtt ctctttccaa tacttcttct ggtgtcagcg
    20641 tataattctc atatttggtt cctgtcacat ttgccagttt gtcataaagg tccattgcta
    20701 ctttgaccga gaacatattg aaggttgttg ctccttcgcc ctcatagatt ggcaaaagca
    20761 ttggatctgg ttttgggatg tgtggggcaa tactttgtac gactgcacgt tcaccaactg
    20821 tgtcagctac tacttccaca tcaaatgtct tcagataacg aataccgcca tgaacaagtt
    20881 tggtcgagcg agaggaagtc ccttctgcaa aatcctgcat ttcgatcaat cctgttttga
    20941 ttcctgatgc tgctgactgg actgccacac cggcaccagt gattccgcca ccgatgatca
    21001 acagatccaa ttcttcttgc tgcatttttt gaatgctatc ttgtcttgtc ttgtttgaaa
    21061 acatatggat ttcctcctta ttcaaatgct tgggtagctt ttactgcttt tttccaaccg
    21121 ttgtaaagtt tttctcggcg ctcttcctcc atttgaactt caaatagctt gccagcttca
    21181 taaaattcgc ggatctcgtc tgtatctttc caataaccga cagctaatcc tgctaaaaat
    21241 gctgccccta gcgccgttgt ttccaagttt tctgctcgtt ggatcggtac attcaagata
    21301 tctgtttgga attgcatcag atattcattg tttgctgcac cgccatctac tttcaataca
    21361 ggaatcttga ttccagtgtc atcttgcatc gtatcgatga tatctctcac ttgataagca
    21421 atcgactgta aagtagcttt aatgatatct tcttttgtcg taccacgtgt caagccaaac
    21481 atagcccctc gtgctgcctg atcccaataa ggagcaccta gcccaacgaa tgcaggaaca
    21541 acataaacct catcttcgct tgtagagcgt ttcgctgcgt cttctgaatc gctagctttt
    21601 tgcaacattt gaagtccatc tctcagccat tgaatcgaag aaccagccac aaaaatactt
    21661 ccttctaaag catagtaaac ctttccattt atgccgtaac caattgtcgt caataaatta
    21721 tttttggata attgcggttt ttcaccggta ttcatgacaa taaatgaacc tgtaccataa
    21781 gtattcttga ccatgcctgg ttcgaatgcc atttgtccga ataatgcggc ttgctggtct
    21841 cctgccatcc ctgaaattgg tgcttcgctt ccataaaaat gataaccttg cgtataacca
    21901 taaacttctg agttagaagt gacttttggt aacatcactc gaggaatatt tagtaaatct
    21961 aaaatctcct gatcccaatc tagctgatgg atattgaaca acatcgtccg actggcattc
    22021 gagtaatcag taacatgtgc ttgtccatcg gtcagtttcc atactaacca agaatcgatc
    22081 gttccaaaca tcaattcgcc tttttcagct cgttcttgcg cgccttctac atgatcaaga
    22141 atccagcgta tcttcgttgc tgagaaatag gcatcgatca ctaaccctgt tttttcgtgg
    22201 aaaaattctt gatagccttc attttttaag ttatctgcaa ttccagctga ctgtctggat
    22261 tgccaaacaa ctgcatgata gattggacgt cctgtatctt tttcccatat tactgttgtt
    22321 tcacgctggt tcgttatgcc aatcccagca atctgatttg gtttgatccc tgattcgatg
    22381 aacgctccgg caataactga ctgtacagaa ttccagattt catttgcatc atgttccacc
    22441 caactttctt gcgggaaata ttgcgtgaac tctttttgag aactgccgat gtgtcttcct
    22501 ttcttgtcaa agataattgc tcgtgagcta gttgtacctt ggtcaatcgc catgatatac
    22561 gttgattctg tcattgtgat tccctccatt atatgcaaac gctttcttta actactctta
    22621 tcatactact gttttaaaaa aagaaactgt caaaatttta ttcttttttt ctttttttga
    22681 cagtttttta ttatgaagct attttttaat caattctttt cttttttcag cgtctctatt
    22741 cttcttttca atcgaataat gtcgtaaggt gacgcaaatt ctgacagata atattcacac
    22801 tgttttttgt ttctctggca aaatgtgatc accaaatcat attctttacc ttcttcataa
    22861 cttccaatct caataccgcc gattcccctc agttctctga tcagatattg ttgaaaagca
    22921 atacgataaa tcggcagact ttctaaatcg attccaatca ctactggttt tgcaatataa
    22981 aaatcgatca tcaaaagaac attggcatac tggatcatca ggtaactaag ctcactatct
    23041 tcagctcttt tcctcggcaa ttgctgcacc gctgtttcct gtaatttcgc cagtaactgt
    23101 gttaaggaat gcccgagcat tttctgctgc tgttccaaaa gccgatcacg ctcatagact
    23161 tcgatctttc caacaaagaa aaaccattca ttgttgatct gggaaatttg gtagcctact
    23221 gccttttctt ctttgatttt caaacgacga ggacgataat gccataacat cgtttcccga
    23281 atgtaagtat ctaaaacagc ggttggtaac tttttgctcc gtgtcaaatc atactgataa
    23341 aatatttctt cgtctactat cgaaaaagaa acaaaaaagc tgtaaaacat cattgtttcg
    23401 tatacattca gttctgctgc tgttcgacta agatgcatca cgatgattgg atcgattgct
    23461 tgataaagcc ggtccgactg atagagtaat ttcttttctt tcaaagttgc gtacgtactt
    23521 ttttcattca atagtctttt tttcgtaatc ccgagccagc aagcaatctg tgcttctgct
    23581 gatgccgtaa acgttgtttc taatacacgg tccaatccta gaatgaacgg tcttttttct
    23641 ggtgtatttt gcaaaaaaag cggtcgctga tcttccggga tatattggaa tagttcataa
    23701 taaaaatatc gtatctgtag ctcttctccg tataattgac cgtttttgat ttgtaatcca
    23761 aattcctcta agagctggtt caattctttg atctttcgaa aaacagaaga ctcactgata
    23821 ttaaacgctg tcgctaatcg aacgatggat acttgctgat gttctaataa atagacgagc
    23881 atcttaaact tcaagctttc ctgcaataaa aacaatagaa ttttttccaa acttacatct
    23941 tcgtccaaca caagcagcgc ctgatactca tttcgctgta cttctacttt tttttccatc
    24001 attcggccaa tcaattcaat atcttccaaa tattgatcaa acgaggcttt tgacagttgg
    24061 acatgctctc tcaaaccatt gatagaggct gttccccctt gcagaacaag gtttcgcaaa
    24121 atcaggatct gtaactgctc ttttctttct aataatgctt caatcttcat aggcagtatt
    24181 cgccaccttt gaccacacca tatctggcac acggtattct ggctggaaaa atagctcatg
    24241 ttcttctaac tgttcaaacg tccatccttc ttctactgca attgtcagtt ggtcaagaag
    24301 gtggaacatc tcttttttgc tttggagctg tgctccaaga atcttttttt cagaatcaaa
    24361 tagaaatttg agtttaactt tgctttgatt tattgctgta agcttgtagt ctacgtatcc
    24421 tgtttcaatt ttttggggat aaaaaatgct ctcttcttct gtcaaaccta cgcttcctaa
    24481 gaaatagcca aaccactgat tgccgactgg acgataggtt ccctgatcct ttgtctgtga
    24541 gccactaatt gtcttgctga catttcgtgc tgtacggaca gcgttattta ctagagaaac
    24601 atacattttt tcatttgttg gacgaaaagt tacttggatc gcatcgccaa tcgcataaac
    24661 gtctttttga gacgtttgca agtagtcatc tacccagatc gttccatcat cattcaatgt
    24721 taattgttct tcccacatct gattgtcggg acgagaatga ttagccagca aaacaagatc
    24781 atagttctcc tttctttttt ccgtctctac aatagcggtt tcattttcgg tcaaagacac
    24841 aacttgttct tctaaaaaaa gtgaaagccc ttgatttttc atttccttta ctaacggttc
    24901 tgtcatttca ggatctagat aacgaaaaag aattgacttt cggctttcgt aaagatggat
    24961 ttgttttttt cttgatgcca acccttcagc tatttcgatt cctgcttgtc ctgcaccaat
    25021 aattgcaatt ttctttgcat tcatgatctt ttgctgaaga gccgttacgt tttgtatctt
    25081 tttaactggg tgaataagcg ttgattctgc agaaatattt cgaaactttt gatttgaacc
    25141 agtcgctaga atcaaacgat caaaatccaa tttttttcct cctgctaaaa cgactttttt
    25201 ttctttcaat cctattactg tcttttctgt gtaaacatgg attcgatagc ggtcagtcag
    25261 ttcttctttc gtgatccaag aaagttgttt ttcactggta acctttcctt ttaatatcaa
    25321 attgaacccg ctgggaatcc atcctaactt cgattgcttt tcaatcaaat atatttcact
    25381 tgttggcatc aattggcgaa gataaatagc cgcatgaatc cctgcaaacg acccgccgac
    25441 aattacaatg ttcatctgca ttcctctttc ttataaggtg ttctactgct aacttctcaa
    25501 tcagaaaaca cagtattcat aactcgcttt caaccttatg atacatagcg atgcagcaga
    25561 atcaaccctc tccatcatac cgtgttctag gtctgcttct caataatgta ctagtggacc
    25621 taattgaaga gtatcatttc tgaagagatg tttcaaaaaa tgtactctta taaaagaaaa
    25681 aaactcgcct tttacgtacc tctgtccaaa taggaaacag aaaagaacga aaagacgagt
    25741 tttagcgtgt gtaatgtaat agttagaata gacacatcat ttatttttaa ttctttaatg
    25801 cttcaacaac tgcttcgcct aattgatgag cagattgagg attttgccct gtaagaagcc
    25861 gcccatcaat ttgaaccact tctgaaaaag ctgctccaga ctgatagttt gctccttttt
    25921 ctttcaaagc atcttctgct aagaatggta cttcattcgt tgtaccattt gcttcctctt
    25981 cttcattact gaacccagtc actgtacgtc cagctaaaat agattttcca gactcatctg
    26041 taactggcaa caatcctaca acaccgtgac aaacagctga gaccagccct tcatttcgat
    26101 agatttgtga agcgactttc ccaagatcta ttgcttctgg aaaatcccac attgttccgt
    26161 ggccaccagc ataataaacc gctgcataat catttgcatc cacatcatca ggacgtaagg
    26221 tatttcccaa ggcccgattt ctgaaatctt catcttcata ataggcccaa tcgatttcat
    26281 ccatattcaa aacactttgc ggttctaaag gtacatatcc gcctttagga ctgacaaaat
    26341 ctgcatcgat cccatttttt actaatacat cataaaaatg agtcagttca cttaaccaca
    26401 aacctgtagg acgctcgatc ttttcgtact tttctacatt tgttaatacg atcaatactt
    26461 tcattttccc atcctctttc tataaaaatt gcgttattga taacgtgctt tcaagaaatt
    26521 atataatggg cttggcaaat aaagctcttt atatgcttct tcaaaaggtt tcgttacaaa
    26581 ttcatcctca cggttttcca ataatttctc tgcccagtca ggatcgacga tcatttgctg
    26641 tccaattgct gcaagttctg catatttcaa tacctgctct acatcttcgc gattgcggat
    26701 cccaccgaca ccaactaaag ggatctttcc ttgaatcgtg ttatggacca cttcaagaat
    26761 cgatttatcg ttgtatttat cagaacgagc tattcgatca tacatattta atgaaacatg
    26821 cagataatct agttttgact cacgtaatcg ttcaagaagc cagatcgtgt cttcaaaacg
    26881 gatacctggt gtttcatatt cttctggtga gaaacgatac cctacgatga atggtttttt
    26941 agcatattgt tcaaccgctg agaaaaccgt tgttaataat tcttcaataa aacgataacg
    27001 attttccaag ctaccgcccc attgatcctc tcgtctattc gaatgcggag agaaaaattg
    27061 ttggatcaaa tacgtatttg ctccatgcaa ctcgattcca tcaaatcctg attcaatcgc
    27121 tctacgtgtg gctttgccaa atgcttggat tgtttcaacg atttcttctt cactcattgc
    27181 tcgtggtgtt tccgcatcag gacgttttgc tgcaactgca cttgcagata caggctgttc
    27241 tccagataaa gttgctcgac ttgtcattcg tccaccgtgg aaaatctgaa taatcgcttt
    27301 tgcgccttct ccttggattg ctttcgctag ctcacttaat cgaggaatca tttcatcatg
    27361 agcgatgctg agttcacctg gccagccttt tccgatatct tggacatttg cagcaccagt
    27421 aataacagca ccaagacctt tagaacgttg tttataataa gtgatctcat cttcggtgat
    27481 cactccattg aaaaagctga gttgtgtcgt cataggcgac agcatcaatc ggttcttcaa
    27541 ttgaactcct gaaggtaaag taattgattg atcaaattgt gacattgtca ttcctcccat
    27601 aattcgctct gattaaccta tcgagaatca tctatcatta aaccactcga ttagttgtac
    27661 atgcaactaa tttgcttaaa aaattttatt ttttttgtaa cttatctgtt gcatcattca
    27721 ttgtctgggc taattcttta cttgcttcat ttcccaaaac tgctttgtat gactgatgaa
    27781 gttcttccca gctaccatcg acttccttag caaattggcg tccttgttct gtaagataaa
    27841 ctaatgacaa gcgaccttcg ctaactgttt tgacgtatcc tttatattct aatttttcaa
    27901 caaaacgagc aatcgttgat ggggcggaat agagcatttg tgcaatatct ttttgtggta
    27961 ttccatcttt ttcttttaat atcaataaaa taaaaccata agttggtgcc agacctgtcc
    28021 ttctaaacgc ttcatcagct aatttattca acatgcgatc tagctttttc acgctaaaga
    28081 aaagacattc atctaacgct ttttcaattg gcttttccat ttcatcccct ccattggcgt
    28141 acatatcata gtatgatttt tattttttgt caaaaaacac ggaataatgt ccgcctcctc
    28201 tgtctagatt tgaaaaaaag aagtagccat cttcaaagaa tttcggcttg ttttcgacaa
    28261 aactccttct ggaacaccgt gaatcagagg ttgtgaaaaa gtcgtttagt tttgagtatg
    28321 aagaaaaatt ttgtgaaaat ggctttccac atttttgcat aaatttggct taataccgaa
    28381 aaactggctt ttgaacaccg tttatatcag aggttgtgaa aaagccgtcc tgcatcaagt
    28441 aataagaaca gccaaacaaa aatagttcct catattttgt cgttttttga gctaatgccg
    28501 atggtcagct gattgtacac cgtttattga gaggtcgtga gcctgccgtt gaactccgag
    28561 tcacaaggag caatttcaaa aaacagcttc tcatgtttta tgactatggt gacttgtgat
    28621 cgaggagttg gcaggagaat actgtttatt cgtaaagaag gggctgtgac aagacttttg
    28681 tcacagtccc ttatttcctt ttattatagg atttcgccat ttgattggat gacttgtctg
    28741 taccaatcaa atgatttttt ctttaaccgt ttctttgtgc cattcccttc gtcatcttga
    28801 tcaacataga tatatccata acgttttgac atttgtgaag tagaagcgct gacgatatca
    28861 atgcagcccc acgacgtata accaaataaa tctactccat ctttgatagc ttccttcatc
    28921 tgctcaatat gctgcttcaa atagtcaatg cggtaatcgt cttgaacggt tccatcttct
    28981 tctactttgt ctatagctcc gagtccgttt tcagctatga acagtggttt ttgataccta
    29041 tcatataatt gattcaaagc aattctcaaa cccttaggat caatctgcca gccccattca
    29101 cttgatggaa gatacggatt tttaattcct ccagttatta agtttccttg gaccccttcc
    29161 caattttctg gatgagcact gactgcacta gacatgtagt agctaaaaga aacaaaatca
    29221 acagggtagg ctgctaagat ttcttcatca cctacttcta tttttaagtt gacacctttt
    29281 tcttcaaaca ttcgttgcgt atacgctgga taatatcctc ttgcttgaac atcactaaag
    29341 aacaaagaag ttctttgctg ctcaattgtt ttttgaatat catccggatc acaagaatac
    29401 ggaacaagaa gctgatagct gatcatacaa ccgacttgtg aacctttgat aatttcatga
    29461 caagcttttg tagctaaagc acttgctata aactgatggt gtgcaccttg gtatttcgct
    29521 tgttccaaat ggggatggtt ttcttcaatt actcctacgc taacaaaagg gtgatgtttt
    29581 acacagttga tctcattgaa agtcatccaa tattttactt tatttttata gcgtttgaat
    29641 actgtttcag cgaaacgaac gtaaaactga ataagctttc gattaggcca tccaccatat
    29701 tctgtaacca aatataacgg catttcataa tgggaaagag aaacgactgg ctcaatccca
    29761 tattttagac attcgtcaaa cacttgatca taaaaaaata atcccgcttc attgggtgtt
    29821 tcttcatcac catgaggaaa aattcttgtc catgcgatag acatccgata gcatttaaaa
    29881 cccatttcag caaataaacg gatgtcttct ttaaagtgat gatagaaatc atttccgtgt
    29941 cttttaggat aataatactt gctgttggga tcctgcgccg cttttacttg ctgatgcgtc
    30001 atcatatgcc attgatctgt ccatttttct ttaggtaaat cgctgtcgaa tgtatacaca
    30061 tcagacacag ataatccttt ttcatctaca tcccatgcac cttcacattg attggccgct
    30121 gtcgctccgc cccataaaaa tccttctgga aattttttca tatcgatccc cctaaacttg
    30181 ctagttttct tgtttgatta tttttcttat gactagtaat gatatttttg aatctactac
    30241 tatttgtaac aataaccatt gtggtcggat catatccctc tttgattaat tcttgcaaat
    30301 ctacttccat caataaatcg cctttacgta ctttttttcc atcagatact gcactttgaa
    30361 agtatttgcc atttaaattc actgtatcaa taccgatatg gatcagtatc tctgctcctt
    30421 cttcactgac taccccaatt gcatgtttcg ttggaaaaac tgttgaaact tccccgttta
    30481 cagaactgta gatttttccg tccgttggaa tgatcgcaaa cccttctcct aaagatccac
    30541 tagcaaatgt tggatcgttt acttcagata aatcaattat ttcacctgct gcaggagtac
    30601 ttaacactat atcctttgag gattcttctt cgatttcatc cttgtagccg aaaagaaaag
    30661 ttagcataca ggaactgatc atcgctaaga aataactagc aactagccaa acaaatcctt
    30721 caccaatata aatcgggaaa gacaaaatac ttcttgtagc aactgccaaa gcaccggctc
    30781 ctgcggcacc agctactcca ccaaatatag ctccacatat acaagcaatg taaaatggtc
    30841 gtttatattt aagagtcaca ccataaacag caggttcagt gattccaaat aatgctgtga
    30901 taaatgcaga taaagataaa ctttttactt tcttattttt cgttttcaaa aataccccaa
    30961 gtactgctcc tgcttgcgca gctacggcag tcatacatgc tggacctagc gtatcacgac
    31021 cgtagaaaga aagattgttg gtcattactg gtacaagtcc ccaatgtaac ccaaaaatca
    31081 ctaacacttg ccaaaatccg ccgataacag caccagataa aacagggcta aagttataaa
    31141 cagctaaata tccagctgca atcagctgac ttgcataatc tgcaattgga cctacaacta
    31201 ataaaataat tgggaccatc acgatgatac aaatgaatgg tgtcaataaa tttcgaatac
    31261 tttcgtgcaa gcttttgcga atcttaggct caatataaga aagaacccat acagctaaaa
    31321 ttgctggaat aaccgtactt gtataccttg ctagaatcac gggcatccct agaaatcgca
    31381 aggttatgct atcactatat gcactgacaa tcgtaggata aacgagagct gctgcaataa
    31441 ccatagcgat gaaacgatct gtattgaact tttttgcagc agtatatgcc aaaaatatag
    31501 gcaagaaata gaaaaatgca tcagctgctg cattcaaaat gatataagtt cctgattcag
    31561 tagttaataa attcaaactc gtacataagg cgagtactcc tttgatcaaa ccacttcctg
    31621 ccaagacgcc taaagtcggg gcaaaaacag cggcgatcat gtctataaaa gagtttaaaa
    31681 tgcctttttt ctcagttcct ctctttccac tagatgcatc atcttctagt ttcatttgtt
    31741 tggataatac gtcatacact tcatttactg catttcctac tacgacttga aacattcctc
    31801 cagcttcgac tacagtgatc acaccttcta gttgctctac cttctcctta tctgccttct
    31861 tattatcttt tagggtaaag cgtaaccttg ttgcacaatg gtaaacattg ctgacatttt
    31921 cttcgcctcc gacaaatcgg acaatatctt tcgctaattg tgaatagtcc atttttactt
    31981 cccccttgtt aattaatgcc gtagcaatcc ttcttacaag ataagtatag ttcttagaaa
    32041 acggtttctc cattcaatta gtaccccttc taatagcgaa cgaaactgta caatagtgtg
    32101 tatggtttac ttagccgagc aaatcactca ctgcttgaaa gaaaacaagt atatcgtttc
    32161 tcttatagtc aaaggagaca aaaaaaacac gcttgatctt cctttgctga tgtataagtg
    32221 gtatctcaag ctgatactct gtgatttttg tttctacttg gcttttgata agacagcaca
    32281 acaaagtctc ggcatttaac ggaagcataa ctaggtcaga actaggagag attagttgct
    32341 gaatggaatc tatcttttta tggattttta tttgattttc ttctttgtag taatgatcaa
    32401 gcagttttgt tttctttttt tcttcaacta tggtcagctt ttcacgcaaa atctgataaa
    32461 tctcccaaga ttcaaactca tctagaacac taggtagata aatcaaatca tcccactcaa
    32521 aaataagctc ttgctcagta gtcaaaattg catcatattc cgttattttg tttgattgat
    32581 ttttgaaaag atacactggt tctatccggc attctttgat ccacatgttc atactttgtt
    32641 gaatctgttt ttttagtgta ttcaaaatac taattggctg gttactaaca atcagtatct
    32701 ccccttgttt tcgaaaagga ttcaacgctt cggcaagata caaaacaaaa taagcgcttt
    32761 cagctaaatt gggacgtaat tgaaaatgta ttgggaagaa acgccgaatt aaataaattt
    32821 ctaaaggata acgggagatg atcttttgta caaaatgatt catggtatta tgtccggcat
    32881 ccatcctcgt tttcatttgt ttgatatggg ttagcaaaaa ttctgtttca tggaacaata
    32941 acgaaacagg aagatctaaa aaacgaataa tcgtttgctc aaatgctctt agattcttct
    33001 ctatttcttt gtttttttca tcattaatca tcaaataatt taactctaat agtcgatttt
    33061 caattgcttg tttttcttga ttggaaaaag aatagggtag ttctgatgat aacttttttg
    33121 tgagggtctc taccatcgga ataaataatg tatacctgtt tgttttttct aaaagttctt
    33181 ctcttcgaag aatggtcaaa acgagatacc gagaaaatcg tatgaagtcc tctcctgaaa
    33241 caatataatt atatgatata agaacctctt ttaatgtctt ttggacagtc ggcatgatat
    33301 tggaaaaaat tgtactgatt tgttgaggaa accttgcgtg ttggatcacc gtttctgttc
    33361 caacaagaga taagaaaatt ctttttgaat tttcttcagc aatgattttt agtccttttg
    33421 tcgacgaaac ttccagttct aatttaggat tgcgctcgat ccaacgcaaa atcgttttta
    33481 tttgtaatga tacggctgtt tttgaaagaa agaaaatgtc tgctatcttt tgcatactga
    33541 catacccttt ttgaaacaat aagtacgtca caatagttga agctcggaaa tccatgctgg
    33601 aatagatttt atgtttcgtc acctcttgta tgatttgctg tttagtttca gattcaattg
    33661 attctaaaaa ataacctgaa cgtttatttg actggataat gattttcttc tcgtagatag
    33721 caagcatatt tttgatttca gtttggatcg ttttcttgtt ggtacctaaa gcttcagcta
    33781 attcttcgct tgttatccaa tgattcttat ttcttaatac tacaagaatt tggagttgtc
    33841 gtttatttaa gtccatttgc ttacacctcc tctttgttat aaacatacag gaaaaacctc
    33901 tcaaatgaca atagtcactt gcagaggttt aaaaatcatt tgaagaaaga tctatattca
    33961 aggtttttac cattagactg gataatttgt ttataatatt caaaactatc ttttttcaat
    34021 ctcctaaagc ttccgctccc atcattttgt ctatccacat aaataaagcc atatcgcttc
    34081 ctcatttccc ctgttcctgc cgatacgata tcgattggtc cccaccataa gtaaccaatg
    34141 atttggcaac catcttttat tgcctcagca atttcctgaa gatgtatttt tagatactca
    34201 caacgttttg gatcgtggat tcgattcaat tcatctggtg cttcatctag tccaatgcca
    34261 ttttcaacta tgaataatgg taattcataa cggtctgtca attcattcat gacataacga
    34321 aaccctagag gatcaattgg ccaactccaa ggttcgggag atttttcctt caaaaaggga
    34381 ttatcgaaac catttgctcc gccagtatct acgcgcattt ctgcttcttt tttatagaca
    34441 gcgctacgat aataactaaa tgcaatataa tctgccgtgt tagcttgcaa gcactgttcg
    34501 tctccatcta acattactgg tgcacagtct tcttccttcc atgtcctgta aatataagct
    34561 ggataatgcc ctcggcacat gacatcggag aaaaaccatg aattgcgttt aaattgcagt
    34621 gaaccaaata cattctctgg atcacagtca tatgcataag tcgctaatgc agaaaagcta
    34681 cacataatac ccatcttagc atttggatcg attttcttta ataattgagt agcacgggcg
    34741 ttcgctagaa acatatgatg acaggcttga taaatcactt tttttgttag tgtagcaatg
    34801 ggttcaagtt tatctctagc agggaatgta accaatccag cagctgcata aggcatacga
    34861 aaagcattat tgatctcatt aaaggtcatc caatatttca ctttcccttg gtagcgttca
    34921 aagacggtct gcgcataaaa ttcaaaaaag tcgattaatt ttcgattttg ccacccccca
    34981 tactctccta ccaatgctaa gggggtttca taatgactaa gagtaattac tggctcgata
    35041 tcatatcgat ttaatgtatt gattagtctg tcataaaata cgagaccagc ctcatttggc
    35101 ttctgttcgt ctcctcttgg aaatattcgt gcccatgaga tgcttgttcg aaaagctttt
    35161 aaacctaact ccttcagtaa acgaatatcc tcttcaaatc gatgataaaa atcaatcgct
    35221 tcatgagaaa gataatggag tgattcgtct aaagcaatct cccaaatctt tttttcttca
    35281 ttccactgaa tactaggatg ctggttcatg atcccgataa gcgtatctgt cacattaggt
    35341 aatttgccgt cttccaacca tgcaccttca cattgattag cagctactgc tccgccccat
    35401 aaaaaatttt ctggaaatat ttcctgatcc ataactatct ctcctcgcta tcaacaattt
    35461 cttacttgaa gtatagctcg attcatcaaa acaagatatt ctaactattt cggcagaaga
    35521 gaagaaaaaa attgaatgct actct
//
LOCUS       DAHHZJ010000011        34920 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.011, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000011 DAHHZJ010000000
VERSION     DAHHZJ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.011"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            595..753
                     /locus_tag="NBL02_000423"
     CDS             595..753
                     /locus_tag="NBL02_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD2571136.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            825..2051
                     /locus_tag="NBL02_000424"
     CDS             825..2051
                     /locus_tag="NBL02_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCD2571137.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(2139..2531)
                     /gene="rpsI"
                     /locus_tag="NBL02_000425"
     CDS             complement(2139..2531)
                     /gene="rpsI"
                     /locus_tag="NBL02_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCD2571138.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(2547..2990)
                     /gene="rplM"
                     /locus_tag="NBL02_000426"
     CDS             complement(2547..2990)
                     /gene="rplM"
                     /locus_tag="NBL02_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCD2571139.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(3509..3976)
                     /locus_tag="NBL02_000427"
     CDS             complement(3509..3976)
                     /locus_tag="NBL02_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HCD2571140.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(4096..5634)
                     /locus_tag="NBL02_000428"
     CDS             complement(4096..5634)
                     /locus_tag="NBL02_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCD2571141.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            5714..6400
                     /locus_tag="NBL02_000429"
     CDS             5714..6400
                     /locus_tag="NBL02_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCD2571142.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(6601..7128)
                     /gene="msrA"
                     /locus_tag="NBL02_000430"
     CDS             complement(6601..7128)
                     /gene="msrA"
                     /locus_tag="NBL02_000430"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCD2571143.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(7212..7889)
                     /gene="pgmB"
                     /locus_tag="NBL02_000431"
     CDS             complement(7212..7889)
                     /gene="pgmB"
                     /locus_tag="NBL02_000431"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCD2571144.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(7886..9016)
                     /locus_tag="NBL02_000432"
     CDS             complement(7886..9016)
                     /locus_tag="NBL02_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCD2571145.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(9016..11199)
                     /locus_tag="NBL02_000433"
     CDS             complement(9016..11199)
                     /locus_tag="NBL02_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HCD2571146.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(11196..12221)
                     /locus_tag="NBL02_000434"
     CDS             complement(11196..12221)
                     /locus_tag="NBL02_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCD2571147.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(12266..13093)
                     /locus_tag="NBL02_000435"
     CDS             complement(12266..13093)
                     /locus_tag="NBL02_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCD2571148.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(13090..14157)
                     /locus_tag="NBL02_000436"
     CDS             complement(13090..14157)
                     /locus_tag="NBL02_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCD2571149.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(14183..15019)
                     /locus_tag="NBL02_000437"
     CDS             complement(14183..15019)
                     /locus_tag="NBL02_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCD2571150.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(15016..15885)
                     /locus_tag="NBL02_000438"
     CDS             complement(15016..15885)
                     /locus_tag="NBL02_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCD2571151.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(15955..17238)
                     /locus_tag="NBL02_000439"
     CDS             complement(15955..17238)
                     /locus_tag="NBL02_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HCD2571152.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(17252..18937)
                     /locus_tag="NBL02_000440"
     CDS             complement(17252..18937)
                     /locus_tag="NBL02_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HCD2571153.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            19212..20237
                     /locus_tag="NBL02_000441"
     CDS             19212..20237
                     /locus_tag="NBL02_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCD2571154.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(20832..24506)
                     /gene="rpoC"
                     /locus_tag="NBL02_000442"
     CDS             complement(20832..24506)
                     /gene="rpoC"
                     /locus_tag="NBL02_000442"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCD2571155.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(24564..28181)
                     /gene="rpoB"
                     /locus_tag="NBL02_000443"
     CDS             complement(24564..28181)
                     /gene="rpoB"
                     /locus_tag="NBL02_000443"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCD2571156.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            28909..29877
                     /locus_tag="NBL02_000444"
     CDS             28909..29877
                     /locus_tag="NBL02_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCD2571157.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            29895..30800
                     /locus_tag="NBL02_000445"
     CDS             29895..30800
                     /locus_tag="NBL02_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2571158.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            30797..31603
                     /locus_tag="NBL02_000446"
     CDS             30797..31603
                     /locus_tag="NBL02_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD2571159.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            31739..32728
                     /locus_tag="NBL02_000447"
     CDS             31739..32728
                     /locus_tag="NBL02_000447"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HCD2571160.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(33045..33569)
                     /locus_tag="NBL02_000448"
     CDS             complement(33045..33569)
                     /locus_tag="NBL02_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HCD2571161.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            33856..>34920
                     /gene="scm"
                     /locus_tag="NBL02_000449"
     CDS             33856..>34920
                     /gene="scm"
                     /locus_tag="NBL02_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HCD2571162.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT        10022 a         7368 c         6526 g        11004 t
ORIGIN      
        1 tgttgctgaa gattagaatg acaaaactat ttgaaactac gcgtatagta agtagctttc
       61 aaaatatata aaaaaggagc gttctcaaat gagaacatgc tcctaaaact actttattgg
      121 cgtatggaaa aattatactt gaagtggata gcgggttgaa taagttgata agcatcaaaa
      181 catttataaa aattattcaa gactatgtct gttccatata caaatatgaa aatcagtaaa
      241 ctccatttaa cttttttcgt agtgtgtctt tttatacatg tttatcgtta ttaatagtga
      301 ataggaaaaa aactattttt gatcattgac aattaaatac aatttttgaa taacgatccc
      361 tgctattcga gctagaaact caagtacgcc atgacctgat tagcgacaaa tacttagaga
      421 tttctttttg ttctgggaaa acaaaatgcc atgacaatag cagcaaataa tgatacttcc
      481 gtaaggctcg ccgtaaactg gggagaggtg aaattcctat gtgcttaccc aaagcaattc
      541 aaaaatttca taactactat tttaggagat gattttatag aatccagagt ctacctgaat
      601 aaaaaacaaa cctgtgcatt tctaggaatc gataataata ctctagataa gtggattcgt
      661 atatataatt tcccagtaat aagagttggt ggtgtagttc gctttgacgt agaagatatt
      721 aaagacttta tgaatacatt taaaataact tgaaattttc ttctcgccgc atatactaaa
      781 attagtgaaa tatactgcct tacttttttt aaggagtgag gattttgcct acaaagcttt
      841 caaatgggaa atataaaact aatctgagat atccaaaaag gtttaaagaa ataacaggaa
      901 ttgcatcaga aaagtatcaa aaaacatttc caaataggca attggcgatt aaagccgaaa
      961 atgatatgaa gaagaaaata gaaaaagttt tgagagagga gaatgcaaac tcattagaat
     1021 taaaaggaaa aataacgttt aaaaaatttt acgaatctaa atggcttcct agatatgaat
     1081 taggtcaaac aattagatca aacaggcctc ctagtgatat aaccattagt aatacaaaag
     1141 atatatttcg gttgcatatt ttacctatgt ttggtgaata cgcaatgaat tatctaaata
     1201 tcaatactga gataatatca gatgaattaa ccaaaaaatc gaaagaatat gcaaatataa
     1261 aaatcataaa aggatatgtt cgttccatgt tcgatattgc agaaatacta aattatatcg
     1321 aatttaatcg aactactaaa attattcaaa gtatcactgc acctaaaaaa aatgctttag
     1381 aagaaaaaag aatacaagaa ggtaagcaag cactatcatc aaaagaactt actaattgga
     1441 tagaagcagt aaatgatgat tttaataatc atttacttac ttttcatgat tacacacttt
     1501 ttatgattac cctgtatctt ggtgatagaa aaagtgagac ttacgctttg caatggaaat
     1561 atattgattt tgaaaaacaa acggtccgtc ttaaacatac tttagataaa tatcaaagaa
     1621 aaaagtttac aaaaggtcga aaagataccg tcattcaagt accagaagtt gtaatgacat
     1681 tgctaagtga atggaaatcg gtacaagctg atcaattgtt gaaattaaaa attaagcaaa
     1741 cgttagatca gtatctcttt acttatacta aaccatctgg agaagtaaat tgtcctgttc
     1801 atgccgatta cctaaactat cgaatcaatt caattaaaag aaggcatcct gacttagttc
     1861 atttatctcc acacaaactc cgtcatactt atgcaactat agctagacaa ggtggagcag
     1921 atatgaatca aatttcaaat gctttaacac actcagatat ttctacaaca aagatatatg
     1981 ttaacacacc agatatagtt gataaggctg tttttgaagc ttttcagaga ggattgaata
     2041 agtgtgattg atatgtgttc ttaaatgtgt tcttaaaaac aaaagaagca ctttccaaat
     2101 aattgaaaag tgccagaaac gttgatatat aaaggatatt aacgttttga gaattgtgaa
     2161 gctttacgtg ctttcttaag acctggtttt ttacgttcga ccatacgtgc gtcacgagta
     2221 agtaagccag cacgtttaag agctaaacgg aagtcaggat caacttctag caatgcacga
     2281 gcgataccat gacggatagc tcctgattgt ccagcgtatc ctccgccgtt tacgttaaca
     2341 aatacgtcat atgcgccttt tgtttcagta acagcgaaag gttgattgat tacttcacgc
     2401 aagtcagcat gtggaatgta ttcttcaaca tcttttttat ttacagtaat tttgccagtt
     2461 cctggtacta gacgtacgcg agcaactgcg tttttacgac ggcctgtgcc gatatattga
     2521 acttgtgcca atgaaattcc ctcctattag attaagtttg tgatgtctaa aacttctggt
     2581 tgttgtgctg catgtggatg ttctgctcca ccgtatacgt tcaattttgt aaattgtttg
     2641 cggcctaaag tgtttttagg aagcatacct tttacagaag tttcgatcaa acgacgagaa
     2701 tttttagcac gcaattcgcc agcagtgatt gatttcaatc ctcctgggta gttgctgtga
     2761 cggtaataaa ttttgtcagt cgctttttta cctgttaatt tcactttgtc tgcattgatg
     2821 acgatgacga aatcgccagt atccaaatga ggtgtgaaag ttggtttatt tttaccacgt
     2881 agtacagatg caacaactgt tgaaagacgt cccaatggaa catcagttgc gtctactaca
     2941 taccatttac gttctacttc gcctggtttg gccatatatg ttgtacgcac gttattttcc
     3001 tccaatttaa gttctgatgt ttgacataca cttcgagttt ccggggctct ttgtggggca
     3061 aacaatacca tttaacatat tacccgctaa tctagtcaat gtcaacgttt ttctgcttga
     3121 ttgaaaaatt tatatgaaac tttacttctt ttctttcttt tatataaaag ggtcgtttcc
     3181 tgtcaaaatg aaaaacaaaa agaggctgtg acaaaagtct tgtcacaacc ccttattttg
     3241 aataaacggt gttctcctgc caactcctcg gtcacaagtc acaatatcca taaaacataa
     3301 gaagctgttt tttaaaattg tttcttgtga ctcggagctg aacggcaggc ttacaacctc
     3361 ttggtaaacg gtgttcaatc agctgaccct cggtattagc tcaaaaaacg gcaaaatatg
     3421 aggaactatt ttgacgtttg tttctctaat actcaggtca aaacgctgat ttcacaacct
     3481 ctttttcggt caagttcaac aacagttttt acgcgtcgat ctctggcgct ttgcccaaat
     3541 gagcttggat catccagata cgtttttctg tggctgtttt gaattcgata aagatatctt
     3601 gtgttggatc gtcgccttct tctccagaaa cttcgattcc tttttggtaa agatctgcta
     3661 gataacgata gcctgcaaca agtgtttcca aacgttctgg aatagtacga tcccagcggc
     3721 caatttcatc agggattttt gtgttatctg caaattcacg caatgtagaa taaggtgaac
     3781 cgtctaatgt gatcaagcgt tcggatactt catctaactg agcgttcaaa tcatccatga
     3841 actcatccat cattgggtgc aatgtaagga aacctggtcc tctcatgtac caatgtgttt
     3901 ggtgaacaac aacagacatt tggcttagat ctgcaactaa ttgatttaat acttctttgg
     3961 tttgttggaa tttcatcttt cttgcctcct aacattttct tacacttaaa ataatagtag
     4021 actttcaatg aaaagtacaa caaaatcatt tacctgagaa cgattttcag ttcgcttttt
     4081 tatccgtaaa gccaatcatt tttcagaatc agctaattct tctcgcagtt gagcaaattg
     4141 ctgataagtc tgtgctaaat gcttttgtgt cgaaacaaat aatggataga ttttctgata
     4201 ttttttatga tcagtttctt gtactccaac ttgctctttc aattctatca attcctgtgc
     4261 ttcactgata ctttggatct ttccgatgct tctccagcct aaaatcacag ctcctaatgc
     4321 ggatgcttct gtgacagtag ggaaaacaag cgggtaaccc agtacatcag ccatcatctg
     4381 acgaaaaatc ggagattgag caaacccgcc tgtagcacgg atctctgtga gctgattttc
     4441 ctggcaaact ccttttaaaa tccgataaag gttgagacag atcccttcta gaatcgcctt
     4501 catcatttct ttttctgtat gttgccgctg taaaccgata aaactgccaa acgcatctgc
     4561 gtcccaaagc ggtgctcgct caccaagtaa atagggatga aaaatcagac cattggcacc
     4621 cgctggcaca aaagcgatag ccgaaagcag tgcatcatat ggattttctc ctttttcttt
     4681 agcagattga acgacttcct gtaaaaatgt atgccctgcc caatcaaaga tgccagcacc
     4741 attgctagtc gctcctccca ctacccaatg tttctgatcc agaacataac aaaacgtctc
     4801 catttctgga tggaactgag gctgagtagt aataaaacga agtgctccgc tcgttcctac
     4861 ggtcaaagcg gcgatcgtat caccaaaagc acctaaccct agattagaaa gcggcccgtc
     4921 tgcccctccc aaaacaaatg atatatcatt tggcaaacct agttctgctg ctgtttttga
     4981 tggtagacct gttacttgat aggtggaagg aaagacttgc ggcaattgct ctattcgaat
     5041 gttcaagtag tcaagcactt cttctgccca ttggaattca tggatatcaa aatatccagt
     5101 tcctgaagct gttccgtagt cgctgatcag ttcgccaaac aatctataaa agatatagtc
     5161 tttgataccg acaaaataag cagttttttt gaataattca ggttggctgt tttgcagcca
     5221 cttgattttg ttcaatggtg tcattggatg aatcggcgtc ccagtctgcc gataaaattc
     5281 tttgccaagc acggtatatt tcagtttttc tgcttcgtcc gctgctcggt tatctgccca
     5341 agtgatcatt cgagataacg gtgttttctc ttcatccaac aatatcaggc catgcatcgc
     5401 agaggagaag gagacaaatt gaatgattgt gtctgaaggc aactgtttaa ttacttcttt
     5461 tataacggtg cagacacctt gaaagatcaa ttcctgatct tgttctgcca tcccttgtgt
     5521 ttcttggatc aaagggtagc gaaaataagc ggatgctgct tcttgggctt catctgtgaa
     5581 agcaactgct ttggtttgtg tggttccgat atctaatccg attgaaacgt tcattttttc
     5641 acctcaaatt ttatgtatgt acaaaaattt agtttaagct tgcgtatctt ggttttgaaa
     5701 agggggagat ggcgtgttac tttattttat cattggttgc tgtatcggat catttttatg
     5761 tttaaccgcc cagcggattc cgttaggtca ctctattatt tatcctagat ctcattgtgt
     5821 caaatgctgt cgttcgcttt cttggtatga attgattccg attctctcga tcatcgtcca
     5881 acgatttcgc tgccgctatt gcaggtgtcg gttgcccttc tactatttgt tggcagaagc
     5941 cttatgtggt ggtctttttg cctggttctt tactttttct tccgcaagaa atttttcgac
     6001 gttcatatgg atgctttcgg ctttgctatt ttcgttgatg gatttatttt acttcatggt
     6061 ccattctcac acgctattct gttcttgggc aattttatgg attttttggt tgcaggccgg
     6121 cgtcttccaa tggcaaagtg tgctgttaac gtttatcgta ggcggtgtgt gccttcgcta
     6181 tggtcaatcc tatctcggag ctggcgatac tctgttgatg ctttcttgga gtggtggctt
     6241 gtctctagaa gagttgcttc agatcttgtt tcttgctagt ctgtttggaa tatccgtttt
     6301 ttctgtttac tttatctgtc ataaaaaaaa gttggaaaag ctgcctttca ttcctttttt
     6361 gagcagtgcg ttgctgattg tccttcatct taggtattga aaaaaggttg tgacaaacga
     6421 cttgtcacaa cctctgatat atagtgttct cttaccaaat ccttgtgact tggagctagg
     6481 cgacaggttc acaacctctc cactaaatgg tgttcaatca gctgaccctc agtattagct
     6541 caaaatgctg gttttacaac ctctgatata cggtgttcca taagtagctt tttcggcttc
     6601 ttatttcttg acgccccaat gtcgacgaag gaaacctgca cgtccagaac cttctcgata
     6661 aagattgtaa tggagcttgt ttttcttgta ataatcctga tggtaatctt ctgcaggata
     6721 aaattcgctt cttggttcga tttttgtcac gattggctta tcgaatcgcc cgctgtcttg
     6781 caatgcttgt tttgaacgct ctgcaatatt tcgttcttct tcatcattat agtagatcac
     6841 tggacgataa gaatcaccgc gatcagcgaa ctgtcctaat gcgtcagtcg gatcggtttg
     6901 ctgccaatag atttccacca actgatcata agaaataact tctggatcat attcgatctc
     6961 tactgcttct gtatgccctg ttgtgcctgt tgttacttgt tcataagttg gatttggtac
     7021 atgcccgcca gtataaccgg atgtcacaga aatgatccct ggctgctctt caaatggttt
     7081 gaccatacac caaaagcatc cgcctgcgaa aactgctttt tttgtcatat aaaatccccc
     7141 ttttttctca tcataccttg atttccaaaa ggaggataac gttttgcttg tgacagaaaa
     7201 aacggaaact attatatacg tgatacggct tctgataaat gccaaaacga acgtacttga
     7261 tcaaaaacgg ttaaatcagg aatcacatat tcagcagaag gaagatttat gttttcacct
     7321 attcccaata catgcatgtt accagccaaa gctgcttgac aaccagcttc tgaatcttca
     7381 atgaccaagc atgcatttgg cggtacattc aattgctgtg ctcctttgag aaaaacttct
     7441 ggatcaggtt ttgcttttga tacttgcgtg ccgtcaataa ggacatcaaa ataacttgtc
     7501 aaattcgttt tttctaaaat gaggcgtgca tttttgcttg ctgatcccaa tccgatcttg
     7561 atgtttgttt ttttcaagta ctcaagaaca gctattgcac caggcaaaac ggatgtttca
     7621 tccatttttt gaatcgcttg aagatataga ttgttttttt gctcagcaaa ggctgatttc
     7681 tctttttctg agaatttctg ttccattttt ccaaatgaca gcaatcgctc tagtgactcg
     7741 gttctggata ttcctttcag ttgctcgttt tctttctcag tgaatggaat ggacagctgg
     7801 ttagctaaaa ccagccacgc ttcgtaatga taacgagctg tgtctacaag aacgccatct
     7861 aaatcgaata gtgctgcttt gattttcatc gattacctcc ttgaaaatga acgacttgaa
     7921 gtaaagatgc gatgttttta gctgtgcgat gaagattatc agtggcttgc tgtaaagctt
     7981 cttgtaaagg cacagctgca ggaaggatcg gaaaaacggc cgaaatgcca acatctgctg
     8041 ctttttcgct tcctttacca acacttccgc aaatagctat cacaggaacg ccctctggta
     8101 ctcttctagc aatcccaact ggcgcttttc cttttagact ttgtccatct agacgtcctt
     8161 cgcctacgat tacgaggtct gcttgctgta cccgtttatc gaactccagt aaatccaata
     8221 cgtagtcgat gccagagact attttccctg aagcaaatgc taacagtcct gcacccattc
     8281 caccaccagc actactgccc gatgcctcga ttacttgagg ggcaaatctc atataaaaat
     8341 catgcatggc ttggtcagcc actgctaatt cattttcttt caagcctttt tgtggtccga
     8401 aaacaaaaga agcgccttct tttccgcata aaagattagt aacatcagta atgacagtga
     8461 ttttggcttt aatcagctca ataggcacgt tcgttttatc aactgtagca attttaccta
     8521 gattttctcc cagtgcttct actttttgat tatcttgatc gtaaaaagca taacctagac
     8581 cttctgccat gccgatgccg ccatcgttgg tactacttcc gccaacgccg atgatgatgt
     8641 gtcgaacacc ttgttgcaac aattgtcgta tcagttctcc tactcctcgc gtggataacc
     8701 taagtggctg ttgttggttt agtgggacat ctttcaatcc gacgatttct gccatttcaa
     8761 aaatcgcagt ttgaccgtct gaagcgtact ggatttcttt gtttttgcca aatgcattag
     8821 taacgactgc tgtttgccgc ttcaacctta aactgtctgc taatgatgaa accgttcctt
     8881 ctcctccatc gcctataggt agcagatcat attcagcctg aacaaaaact tcttgaaaac
     8941 ctgattgaat ggcttgtgct gcctctaagg ctgtcagact ttctttgaaa gaatctggcg
     9001 caatcagaat acgcattatt ttcccctccg ttccaagatc agttcttttt ccagctggta
     9061 ttggtctcca ttgacaaaaa taggtaatgt ttctttcgtc tcttttttta ttactatttg
     9121 tttttttgtc aaaacaaata gcaacctgtg atttttccaa ataaaaggaa aagaaatttt
     9181 ttcccatgct gaaggaagtc ttggagaaat agaaagagtt ccattcgtta ctgtaatacc
     9241 tgaaaaacca aatataactg ctagccatag tgctcctaat gaagcagcat gaattccgtc
     9301 atctgatgaa tgcggatctg gtcctaaatc aatcaggcac gcttcttgga ataaatgata
     9361 cgcccattcc atttcttgga tacggcaagc ctctatggcg tgaatcgctt tactcaacga
     9421 tgagtcgtgg attgttttgt tttcatagta atgcaaattc ttagaaacga ttggttgaga
     9481 aaaatactca ggtaataagt ataacagcat gacaagatct gcttgcttgc tgacttggag
     9541 ttcatttact tcgctacggg cataatcaag taaaatagac tggctgccag gacgttcttt
     9601 gtaagtggtc aaatcaataa ctggcttact gagatacgtg tcatcttgcg gaatcagctg
     9661 ttctttgtta ggcactggca aatataactt atctaaaaaa ttttgacatt ctttcaaaaa
     9721 aagcggatcg ccagcttgat atttaacagc taaggccaca ttataatgcg ccagatagtt
     9781 cgtatatgca ttattgtcga tatattctgt gtattcatct ggaccaatga ctctgtgtat
     9841 ctctaaatga tcatcgactt ttacagctct gctcagccaa aaagctgctg tttcatgaat
     9901 gagcgttttt ccctttgttt ctaaaaacga ttgatcctct gttgcttgga catactcttg
     9961 aacggcataa gcaaggtctg cactgatatg atgttctgcg gcagctgaag caacttcttg
    10021 tcttgttccg gttttgatat tcaatgcagc aaatttgggt gtctcttcct gaccagtgtg
    10081 tgcgctttcc catgggaaaa gtgcaccttg atagtgataa tctcttgctt tttgccgtgc
    10141 gccttctaat cgatcataac ggtacagcag caaattcttt gctacttcag gaaactgata
    10201 taaaaagaaa ggcaggataa aaatttccgt atcccagaaa acatgccctt tataaccttc
    10261 gccagttagt cccttcgccc ctacactaac acttggatca ttgcggggag tcataatctg
    10321 taaatggtaa gcagcgaagt ccatcgctaa ctgatcgaaa gaagaatcgc cttggatcgt
    10381 tatccgatgc tgatgccaaa aatctgtcca ataagacatc gactgctcta aaatttctgt
    10441 ataactaacg cttttctggg cttgggcaat acttttctta aacgggtctg tttgctgaag
    10501 agaggtagag agcacgatga tcttctccag cgtaaaggac gtattttttc tgatcttttc
    10561 tgaaaaggaa gcggtgatcc ggcgattttt tgcttggaat acacctgctt tattgaattt
    10621 agctgcaatc ccaatatcaa ttttactttc tgttgtctgg taggatgcac tgatcaaatc
    10681 gtcaccaaat actcgcagtt ctttttcaac caactgctgt gtaccaaaat tggtttgctg
    10741 tccatcgata cccgttttca catctacttg gcagtcttcg gtcaacggct tgatgaccac
    10801 tttcagggct gcctgatgga gattattttg atcagcaaca cgacaagtaa tgacttccac
    10861 tttttttcct tgagcagttt caatgagcgc atagcgttta agctctccca tttctaaatc
    10921 tagtgcccgc tcgtatgcaa gaactttcat cgtctcaaga gaaaaatgtt caccgtcgat
    10981 ttttatttcc catctgatga catctgggag attcactaac tcggctgatt ctgctcctaa
    11041 aggtcggtta tatacaccag ctgcaaacat tccccttact tgattccgat agtcttcctc
    11101 ctggcttcct ctgataccaa aaaatccatt tccaatggtc atctgactcg catacttatt
    11161 gatatactct aaatcaaact gtttttcttc tactttcata aggtaataac ttctcctgtt
    11221 tctgccgaac gatataaatt ttccaccaga ttctggatga catatccttg ccagccatct
    11281 gcaatcatct gagtctggtc accgagcgcc cgattcacaa aatgagccat acttagttca
    11341 tgtgggtcat taactggcgt ggtcttttgt aaggaaacca gctttccatc tacatcttcg
    11401 aatagttcgc ctggatacag attagcacct gcctgatcgc cacaaaaagc cacattcagt
    11461 ttgtcttctc tgatattcaa agcaaacgaa gtattcagtt ggatgatctt gccgttttct
    11521 aattccagtg tgccaaaaat tgcatcttct actgtgaatt tgcttggatc ccatttcccg
    11581 aatttccctg aatttttttt cgtaccaatt ttttggaagc tgtacgcccg aacgcttttt
    11641 actttaggaa atccagtaat atacatcgct gtatcaagca tgtgaatgcc ataatcgatc
    11701 aaaggcccgc ctccttgaag ttctttgtcg ataaacgttc cccagcctgg aacaccagac
    11761 cgacgaagcg cattcgcttc tacgtaataa atatccccga gaacatcaag atgattcttg
    11821 atcattgttg cttcgtcaga aaaacgatgc tggaatccat aagccaaaat ctgcccgttt
    11881 ttatcggctg tctctgccat ctttcttgct tgatcagctg tcatagctgg tggtttctcg
    11941 cacatcactg aagcattagc ttcgagggca gccaatacat gttcgaagtg aaagcgattg
    12001 ggtgtacaga cactcacgat atcgggagaa atctcattca gcatttgtgt aactgatgta
    12061 tacgcatgag aaaatcctgt acgtcggcaa aagtcttctg ctcgctggct gactggatca
    12121 accacagcgg ccaattctaa gacatctgat cttttttgat aataagcagc atgtactttt
    12181 tctgctactt gtccagcgcc tatgattgct actttcttca tttatttctc cctcctttgc
    12241 ttatttactt attgttattc tttctctatt ctttctctaa atcatttaaa tgacgcgctg
    12301 tttccagaca gtttttcaag tattgcaatg attcctgata aattttcact tcattttctc
    12361 ctcgcaaacg acattcatat accatataac cttcatactc attttgaagt agcgtttgaa
    12421 aatggcgagt aaaatcaatt gcgccagatc ctggttggta gcgatgatta tctgctaaat
    12481 ggatatgacc cacatactct ttttgcgtag tcaatgcttt tgaaatatca tcttcttcaa
    12541 tattcatatg ataaaaatca gcgatgatct gcacatgttt caattgatta gagtcaatgt
    12601 aatgacgcgc atccgctaac aaattgatca tatggtcttg gtatcggttc aaaggctcca
    12661 aataaataac cgtacctgtt tcttcggcta ctttatctaa ataacgaagt gattcgctga
    12721 ctgctttata gtctccttct ttgcttcggg gtgaaaccat cggcggtaat cgataagtga
    12781 acattcccca agctgctggg ataacgattc ctttgcctcc aacctcgctt agtgcacgca
    12841 aaatctcagc aatttgacgt acaccattaa gacgccgttc ttcgataaag tcaccgatcc
    12901 acccatcgta gccgttacaa gctgtcacta ctgggatccc tgtttctttg attgcctgtt
    12961 tgactgtgtc gatattttct attaacaact taccgtcgat ttcgtattct tcaaatccac
    13021 agtctttgat aaatctaaat ttttctaaaa tgtctcttgg gaaaaatgct tgatcttgcg
    13081 taccgaattt cattgttctc ctcctcggac ttgaacgccc attttgatac tttcttctgg
    13141 gtgctggtct acataacgca tgtaagcttc tgcgctttct gcaaatggca caactggatc
    13201 aatgatttct tcacaatcca agtaaccgtt catcaataat tcccaacatg tgtcctcgat
    13261 tcgtttacgg ttccatcttg ggtaatctgg gtttggttca ctagatgctc gagcaaagac
    13321 gatttttgca ttattgaaat gtgcttcgcg accaagatta aacccagctg gaaaaggttt
    13381 agcaaaggca acataagaaa tgatcccgcc atagccgatc cctcttaaag aagcttgtaa
    13441 tgcggcagca ctgccactcg tttcaataac tgaatctact ccacgtttat ctgtcaattc
    13501 ctttagacga aaaccgacat ctgctttcat aggatctagc gtgtaatcgg caccgtattt
    13561 ttctgctaat tctctacgct gcgctatggg atcgatccca acgatggtag tagcacctgc
    13621 ttttttagct aattgaatcg caatttgccc gatagcgcct aaaccgaaga ttgctacagc
    13681 atcacctgct cgaacatgtg cttcccggat accacctaaa gcgaattgag caggatcgta
    13741 acatactgca tttttccatt gatccaaccg atcaagttta cgtagtttat aattatcaac
    13801 accgtttgta atcagcgttt cacgaatggg tccataagtg cagactaact ctcctttttg
    13861 atactctgtc accaaactgc ccgtttcttc gatcgtaccg acaatcatat ttcctaattg
    13921 aaattcgcca aataccacac cgcgttggct tccttcttct cttggcaaga ataggcgcca
    13981 ttcttctgaa aattcttcat cgataaatgg acttaatcct ctaaagtcga caatttctgt
    14041 tcctggtttt ggagtagcga aaattactcg gatacgtact tcgttctttt cgatctcacg
    14101 gtcttcgtaa tctttgaatc cggctattct tggttctaat gcaactaatt ttttcatctt
    14161 ttctttcctc tttcttttct ttttatcctt ttacgccgcc ttcgactaaa tcacctttaa
    14221 taaatcgttc agataacgca tacataatga tgactggtaa agcggtcacc aatgaagcgg
    14281 ccatcattcg tccccaaatg tagtctggtg tactgaataa tgtgttcaaa ccgattggta
    14341 aagtaaattt ttcggaggtc gacaggaaaa tcgaagcaaa taaataatca ttccacgaaa
    14401 ccataaagca ataaacgaag acagaaatga ttccagaaat agccaatggt ataatgatcc
    14461 gaataatgat ctggatccgg ctaagaccat cgatcattgc tgcttcttca atatcagtcg
    14521 gaatcgtgtc aaaataactt tttagcatga atatcgcagt aggaagtgtt tgaacgacca
    14581 tcgtgataat cagtgctgta ttggtatcat acaaacctag accggaaatg attttaaata
    14641 atggaacaac gatcaaaata ccggaaaaca tataaacggt gtaaaagcta gcattgattt
    14701 tcgagcgagc tttgaagcgt agttttgaca aagcgtatgc cccgctgatc ccaatcacta
    14761 ctgcagttac ggaggctatc aaggagatca ctaaactatt tttgaaatac atgatgaatg
    14821 gaaaaatctc agcattgaaa atatcctgat aatgttcaaa agtccattct ttcggaaaaa
    14881 tagtttgtgt cgttgcaatt gcttcttgtg aatccttgaa tgatgtcatc agcatgatca
    14941 aaaatggaaa caaattgaca gctaatagca acaaaatcgt cgcgtaaaat aagattttcc
    15001 tgctcttttt atttttcatt tcaagtccac cttctttcgt gagagcaaga cgaccgctgc
    15061 aataatgatg aacaatagaa tcgatatagc tgctgctttt ccaagatcat tgaacgcaaa
    15121 ggctgtctga tacaaataaa cgcctaagat attgacttta ttcgtcagta gatagacatc
    15181 tgtaaacata tagaacatcc agatagatcg taaaacaatc acagttgcta gtactggttt
    15241 gattgccggc agtgtcacga tcttgaactt ctgccaagca tttgcaccgt ccatttcagc
    15301 tgcttcataa agcgttttgt cgactgtttg caaaatagcc aaaaatgaaa taaacgcata
    15361 cggatagtat ctccagatag caaacaaaac gaccataaag aagctagcaa ctggctggtc
    15421 gaaccacagc ggtgcttcgt tgaaaagctg caacctatct actaacagat cattgacgat
    15481 tccataacta ttattgaaca tatatttcca agcaaaaatc aaagaaatag atggcgtaac
    15541 ataacttaat aaaatcagtg aacgcaccgt tttcctaaat ctgaacttgc ggttaagcaa
    15601 aatagctacg gctaatccta aagctgtact gcctgctaca actaagaatg tgtaagcaac
    15661 tgttaaacct aaagaacgat aaaattcact atcacttaaa atttcaagat aattgcttaa
    15721 tccgataaaa acggcgtctg agttaggatt cagcggcata tcgaaaaaac tgatctgaat
    15781 attggaaagc attggaatca gcactaaaac agctaataaa agcatgcttg gtgctaaaag
    15841 tcccatggct aattttaagt cagaatgttt ccttggacga ttcattgaaa cctccccctg
    15901 ataatctgcc aatagctatc tacttttttc gaacaccatt ggcagaactt cttgttattt
    15961 cgtatttttt tgtgcgtcag ctaaagcttg atcgacatct tcgtttccga cagttacttg
    16021 gttcactgct tttccgatca cacctgagct tgtgatatct cccattttcg tgaaattctt
    16081 ttccccgact agtccaaaga cttgtacttc atcaaaagca ctagcaatct cagatggcag
    16141 ctcgccaaaa gacttgatga cttcattttc tttgtaacca tccagttcga ctacttgttt
    16201 attcactggt tgagcaccac caggagacat caagacccac ttttccatgt ttttcggttg
    16261 agataagaaa gctgtgaatg cttcagcagc ctctttttgt ttctcgtcta gaccagaaga
    16321 aatcgtcaat ccagatactg tgccatagac agcttgatct ttttgtgttg ggatagcaaa
    16381 accgatattt tctgagttcc ccgcttcata aacagaaggc aaaatgtaag ttgaatagat
    16441 tgccattggt gctgttccat tcatgaaagc atctttgatt tctgtcacat cattagagcc
    16501 tggcatcgtg tattgggata agttttgata aaacgacaat gcttctctca tcttttccgt
    16561 atcgatcgtt acctcgcctt catcatccaa aacatttgct ccatttgaca aagcaaattg
    16621 agagaacgct tgctcactca ttgttccttc agcagtaggc atagcaattc catattgttt
    16681 attctcttta tctgtgaaat gttgagcaat ttttaaaata tcatcccaat tttctggttc
    16741 tgaaaaacca gcttctgata atttttcttt gtcataccaa ataccttgaa cccagccgct
    16801 aattggtgca gcaatatagc tttttccatc ttctgaacga acaaggtttt tggctccgtc
    16861 ataataatta tcttctccag cttcctcgat aacgttttgg actgccgtct gatcaatcag
    16921 ttcatcttta tccatcactt tagcaaaatc ctgactgact tccattactt ctggtaattt
    16981 accggaacga gctaacgtaa cgatttttgt attgaacgct gattcttcta ccggcacttg
    17041 cttgattttg atgtctggat tttctttttc aaaatcagca accagttcat tgatcacact
    17101 taatcgttcc tgttcgacag aagagtgcat gaattgaatc gttgtctgtt gtgattcgtt
    17161 agagctttga ctttcaccac aagcagtaag aagaaatcca gtagctagca gacaggtagt
    17221 aacaataatt tttttcattt ttttccacct cttattttat tattttcagc cacatatatt
    17281 catacggctg gaatatcaac tttcgttctt gactgcaatg gataatttgt ccagaaaaca
    17341 ggtctaggta atctcctttt ggtaaaagat aacctgctga ttgattggaa aaattatgga
    17401 taactaagac tttctcttcc ttcgcttcgc gaataaaact gataaaagaa tctgctgagt
    17461 ccagtaagta aatcaaacta ttcggatgga aggctttttc tttttttcga atagatagca
    17521 tctgggtcaa ttcaccatat actgctgttc ttaatggatc tgtctgcaac tctttctcaa
    17581 tatctgctaa caaaaatttt tgccgattga tttttcgatt ctctcctgtt ttttctacgc
    17641 ctttgtaatc atttcgactt cccagcacac tttgaatata aattgccggc acgcctggta
    17701 acgttaccaa aaatgaatga gcagctaaaa agcgtttgct tctccattcc gtcgtatctg
    17761 ttggagaact taacgcatct agatacgtag cgtttatctc atatggactg gtcgttccat
    17821 ctgggttttt cttgtagttt actcgggtac cctctttctt cagctgttcc actaattcaa
    17881 ggatcgtttt ttccggcaga atacctcgta ccggattcaa tccgataccg tcatgggagg
    17941 ctaaaaaatt aaaaaaagtt gtttttttcg atgcgaaatc gatggttttt aaccattctt
    18001 tcaacttact tgtattaccc gaacgaatcg cttgaagggt caaaggtggt agcgaaaact
    18061 gatagaccat ctgtgcttca ttctcgccat tcccaaaata actgatatta tctttgtgtg
    18121 gcacatttgt ctcagtgatc aataccgttc ctggagctgc ttcatcagcg attgcacgga
    18181 ataattggat gattttatgc gtttctggca ggtgtataca agaagttcct agttctttcc
    18241 ataaaaatcc cacagcgtcc aagcgaataa atgaagcgcc tttttctaag taaaacaaaa
    18301 gaatatcgat cattttcaat aaaacttctg gatttttaaa attcaagtct acttgatctt
    18361 cgctaaaagt cgtccatacg tgtttttctt tcccgttttt gtcaatgaac gttgtaagta
    18421 acgggctggt ccttggtcta gtcacgctgc ttgtatctgt atccagtggc aaatcaataa
    18481 aaaaatcttt gtacgtatca ttcaagttta aatattcctg aaaccaagta ctttcggcag
    18541 acatatgatt gcagacaaag tcgaacatta accgtgtttc gcttctcatt tcttctatat
    18601 cttcccatgt gcccaaacgg tcgttgattg cctgataatt tacaacggag aaaccatcat
    18661 cagatgtata tgggaaaaat ggcaagaaat gtacaactct aaatgtatct gccaaatact
    18721 tttgataaaa tttcttaaat gtacgcaaag ttggttgctg ctcttcaaaa aattgatccc
    18781 cataagtaat caagaaaata tcttcttcgt cccaatgcag tttgcctgcc tggcctttct
    18841 tctgctgata aacactgact ttttgttcaa tcatagaggc ggttttctca gacaaatctg
    18901 agtcgcgata aatcgcttgt aaaagttttg tatacatcgg attccccttt cttttttggg
    18961 agcgttccca acgatttgat catattatgg aaaacgctta ctgtcaatag cattttttta
    19021 aataattttg atatacacaa aaatacaaat aagaaagctt atgtatcaat aattaatagc
    19081 ttcttaaaaa aatatggtct ctcatgatca tgcaagctat tcctaaattt tcaaaatcat
    19141 agacaaaaaa gaatgtctat tgtactatgg aattattcta aaacgctccc aaaaaggagg
    19201 tgcatataaa attgaaacct actatttatg acattgcgaa aaaagctaat gtctcaaaat
    19261 caactgtctc cagagtatta aataaccagc caaatatctc tgataaagca aaagcaagtg
    19321 tacttcaagc aatcaaagaa ttgaattatg aaccgagcaa aattgcaaga ggactgtcag
    19381 gaaatggttt tgacgcgatt ctagcattat ctcatcgtac ttctcatacg acctatggca
    19441 atcctttttt ttcagatgtt attcaatcca tttctgccgt ttctgaacaa aataacttcg
    19501 acttgattct acaaacggca aaaacaccag aagaagagct agaaaaatgc gcgaaaaaga
    19561 tccaagaaaa aatgatccga ggaattatta ttttaagctc tacgatggat gaaacacttc
    19621 tttttgaatt ggatcaatac aatattccta ttgtggtaat cggaaaagtc ggaaacgact
    19681 acacgaacat ctactcagtg gacacggata atttccagga tagttacgat ttggtggaaa
    19741 cactgatcaa gaatggacac cgtcatatcg gctgtctcca tgctccgaca gaagtttatg
    19801 tatccaatga tcgtctcaat ggttaccgtc aatgcttatt tgatcaccac ctagatatcc
    19861 gtaatgaatg gattatagac ggcggctttc gcttagaaga tagcatggca gcaatgaatg
    19921 atttaatgaa gctacgagaa agaccaactg cgattttttc taccgatgca ttaaaatcat
    19981 tgagtattta caaagtggca gaacaatctg gaaagcagat acctgatgat ctttcgatcg
    20041 caggattcag tgatccctta ttgtcctccc tattttcgcc acagttgact cggatagaca
    20101 ttccgacaaa tcagttagga atcaaagcca cagaactttt attccaacta atccaccacc
    20161 aataccctga agaaaaaaga atcattcttt caaccgagat tactctcaca gattcgatcc
    20221 aacaacaaga aatgtagaac agaattataa aagaacagaa acactgagga taagtcagtt
    20281 actctgttct tttttcaatc actcatccct cgttaccccc tcccggtatt ttagcgaatt
    20341 tttcttattt tccatctgtt ttagatagct gtggataact ataatgtttg tgttaaacta
    20401 ttttttccac agggaagaaa aaactatttt taaccacgtt gtgtataagt ttttatagtt
    20461 cgttttttta aacacattta cccttatttc aatccatgtt atttggggat acttatctct
    20521 aaaacataaa tagaataaca gacatgtcta ctggttttgt tttattcgag gaattattca
    20581 ataatagcta gcaaaatatt aaaaataacc gctctatctt cccatcaatt tattttgcat
    20641 tcaaaaaatg atctatcttt acttttgttg attgacttct cttggaagat gatcgttccg
    20701 ataccttaat tggatacgaa ccgatgttgt taagatcctt tcccactttt cttctagtta
    20761 gacaaattat tttagacaaa ccaaaaaaga ggttgtgaca ataatttgtc acaacctctt
    20821 ttttaaaata tctattttgt ttgattgttc attgcgtctt ctgcagccat ttgcgcttca
    20881 atatctgaaa tactgtagac attttcgctt gctacaccga cttcttttgg ttccatgtca
    20941 cgataacgtt gcataccagt accagctggg atgatcttac ctataataac attttctttc
    21001 aaaccaagaa gaggatcttt cttaccgcgg atcgcagcat ccgtcaagac acgagttgtt
    21061 tcttggaatg atgcagcaga taagaaacta tttgtttcta atgatgcttt tgtgatacct
    21121 agtaagacag gtcgagaagt tgcaggaacg cctcctgaga ctagcgtatt gtagttttga
    21181 tctttaaagt ctgcgatgtc taataatgta cctggtaaga tatctgtatc acctggatcc
    21241 attacacgaa ctttacgcag catttgacga accattactt cgatatgctt gtcgccgatt
    21301 tctacccctt gcatacggta aacacgttgt acttcacgaa gcaagtagtt ttcaacagat
    21361 aatacatcgc gaacttgcaa caattgtttt ggatcgattg atccttctgt caatggtgca
    21421 ccacggtgga tcatatcgcc ttctgctact ttcatacgtg ctgtataagg aacagtatac
    21481 gtacgtgtat ctgtttttcc tttgattgtt acttctttgg tacgtgttgc aggatcttct
    21541 gagatatcga tgacatcccc tgttacttct gtgatcactg cttgcccttt cggattacgt
    21601 gcttcaaaga tttcttggac acgaggaaga ccttgagtga tatcgtctcc ggcaacccca
    21661 cctgtatgga atgtacgcat cgtcaactga gtaccaggtt caccgattga ttgagcggcg
    21721 atcgtaccaa ctgcttctcc gacttcaacg tcagagccag ttgcaaggtt gcgtccgtaa
    21781 caatgtttgc agacaccatg ttttgtatta catgtgaaga cagaacggat tgtcactgtt
    21841 tcgacacctg catcaacgat ttgttttgcg atgtcttcgg aaatcaattg gtttcctgca
    21901 atgatcaatt catttgtttc aggatgacgt acttctttac gggtgtaacg tcccactaaa
    21961 cgatcttcta atgattcgat gatttcattt ccctcgcgga tggcttggat ttcaagacct
    22021 cggtcagtac cgcaatcttc ttcacggatg attacatctt gggccacgtc aaccagacga
    22081 cgagtcaagt aacctgaatc ggcagtcttc aaggctgtat cggtcatccc tttacgagcc
    22141 ccgtgagttg agataaacat ttccaagacg gatagtcctt cacggaagtt cgaaatgatt
    22201 ggcaattcca tgattcgacc attaggagcg gccatcaaac cacgcattcc ggcaagctgt
    22261 gtaaagttgg aaatgttacc acgagctcca gaatcagaca tcatgaagat tggatttttc
    22321 gcttccaaac cttccatcag tttttgttgg atgctgtctt ttgcagcatt ccaaacagca
    22381 atgacacgtt cataacgttc gtcatctgtg atcagaccac gacggaattg ttttgtaata
    22441 gtttcaactt gtttatgggc attttcaatg atttcttgtt tttcattcag tacactgatg
    22501 tcagcgatcc ctactgtgat accggcatgt gtcgaatgtt tgtaccctaa gtctttcata
    22561 cggtcaagca tcttagaagt ttctgttact ttgaatcgtt tgaagacttc tgcgatgatg
    22621 tttcctaagt ttttcttctt gaacggtaat actaattctt gctctttgat atgtgcagga
    22681 atatcagtac cagcttcaac aaagtatttg tccggtgttt ggacggtcaa gttgaagtct
    22741 gttggttcat tcagataagg gaattcagga ggcatgatct cgttgaagat gatcttacca
    22801 actgttgtga tcatcgtacg ttctttttgc cattcagtaa atggtttttc acctaatgag
    22861 ttagggttca caccgatacg tgaatgtaaa tgaacatatc cgttgcgcca tgcgataacg
    22921 gcttcgtttt gatctctgaa gaccattcct tcgccttcgc gtccttcttc ttccattgtc
    22981 aagtagtagt tacctagaac catatcttgg gaaggagtaa caactggttt accatcttta
    23041 gggttcaaga tgttttgagc agctaacatc aacatacgtg cttctgcttg tgcttcttca
    23101 ttcaatggaa cgtggacagc catttggtct ccatcgaaat cggcattata agcttcacat
    23161 acaagtggat gcaaacggat tgcgcgtcct tgtactaaaa ctggttcgaa tgcttggata
    23221 ccaagtcggt gaagcgtagg tgcccggttc agcaatactg ggtgttcctt gattacgtct
    23281 tctaaaacat cccatacttc gtcttcttga cgttcgattt tacgtttcgc atttttgatg
    23341 tttgaagcta gttcgcgttg aacaagctca cgcatcacga atggtttgaa taattcaatg
    23401 gccatttctt ttggaagtcc acattggtac atcttcaaga aaggtccaac aacgataacg
    23461 gaacgacctg agtagtcgac acgtttacct agcaagtttt gacggaaacg accttgtttc
    23521 cctttcaaca tatgagaaag agatttcaat gggcggttac ctggtccagt aactggacgt
    23581 ccgcgacgac cgttatcgat caatgcatct actgcttctt gcagcatacg tttttcattt
    23641 tgaacgatga ttccaggtgc attcaagtct aacaaacgtt tcaaacggtt attacggttg
    23701 attacacgac ggtaaagatc attcaagtca cttgttgcaa aacgtccacc ttctaattga
    23761 accattgggc gtaaatctgg tggaataaca gggataacgt ccataaccat ccagcttggt
    23821 tgatttcctg aagcgcggaa tgcttctaaa atatccaaac ggcggattgc acgtgtacgt
    23881 ttttgtcctt gtgcagattt caattcttct ttcaattcgg ctgcttctgc atctagatca
    23941 acattatcaa gtaattgttt aatcgcttct gcacccatag cagcttggaa ttcttgtcca
    24001 tattgttcac gtttttcgcg atattcgcgt tctgtcaaca attgtttttt ctctaaattc
    24061 gtatttcctg gttcgatgac tacatatgaa gcaaaataaa tgatttcttc caaagcgcgt
    24121 gggctcatgt ctaatacaag acccatacgt gaaggaatcc ctttaaagta ccagatatgt
    24181 gaaacaggag ctgctaattc aatatgtccc atgcgttcac ggcgaacttt cgaacgagta
    24241 acttccactc cgcatcgatc acaaacgatt cctttataac ggatacgttt gtattttccg
    24301 caggcacatt cccagtcttt tgtaggacca aaaatccgct cacagaacaa cccttcgcgt
    24361 tcaggtttta aggtacgata gttaatcgtc tcaggttttt ttacttcacc gtaagaccag
    24421 cttctgattt tttcgggaga agccaaacct atttgcatac tttcgaattt atttacatcg
    24481 atcaaaaggg gttccctcca tttcatttaa tggcaaaccg caagacggct gatcttcgac
    24541 cgccttgcag ctcatagacc ggattagtct aaattatctt ctgcggtttc aaagttttgg
    24601 atgacatcgt ctttttcatc ttccacttgt tcagcagctt tcttttctaa ttctttggca
    24661 gtttgctgtt cggcaaattt tgtcaaagca tcaacagtga tcaaatcgtc gtcttcatcg
    24721 tccatgtcgc gaagttcgat ctctgtttct tcgatatcca agacacgcat gtccagacct
    24781 agtgattgca attctttcac caatacgcgg aatgattccg gtacacctgg ttttggaatt
    24841 ggttcaccct tgacgattgc ttcgtatgtt ttcacacgtc cgactacgtc atctgatttg
    24901 tatgtcaaga tttcttgtaa agtataagcc gcaccgtaag cttccagtgc ccatacttcc
    24961 atttcaccaa aacgttgtcc accaaattga gctttacctc ccaatggttg ttgtgtaacc
    25021 aatgagtaag gtccgattga acgtgcatgc aatttgtcat cgaccatgtg agcaagtttg
    25081 atcatgtaca tcacaccgac agacacacgt ccatcaaatg gttcacctgt acgtccgtcg
    25141 tacagaacag ttttcgcatc gctggccata ccggcttcac gaactgtttc ccatacgtct
    25201 tcatcacttg caccatcaaa tactggcgtt gctacgtgga tacctaactg acgtgcagcc
    25261 atacccaagt gtaattccaa tacttgcccg atgttcatac gtgaaggcac ccctaaagga
    25321 ttcagcatga tatcgattgg tgttccatca ggtaggaatg gcatatcttc ttccggcata
    25381 atacgggata caaccccttt atttccgtga cgaccggcca ttttatcccc ttcatggatt
    25441 ttacgttttt gaacgatata aacacgaact aacatgttta cgcctggtga caattcatct
    25501 ccagcttcac gagtaaagat tttcacatcg tgaacgatac cgccgccgcc atgtggtaca
    25561 cgtaatgatg tatcgcggac ttcacgagct ttttcgccaa agatcgcatg caatagacgt
    25621 tcttctgcag ataattctgt tacccctttt ggcgtgactt tccctacaag caaatcgcca
    25681 tcttggactt ctgcaccaat acggatgata cccatttcgt ctaaatcttt taatgcgtct
    25741 tccccgacat tagggatttc gcgtgtgatt tcttcaggtc ctaattttgt atcgcgggct
    25801 tctgattcat attcttcaat atggatagaa gtgtatacgt catcttttac caaacgacgg
    25861 ctcatgatga tcgcatcttc atagttgtac ccttcccaag tcatgaaacc aactaaaacg
    25921 ttttggccta gagccatttc gccttgttcc atagatggtc cgtctgccag agtatcccct
    25981 ttttcgactt tctcacccaa atgaacgatt ggacgttggt tgtagcttgt tccggagttt
    26041 gaacgacgga attttgttac atcgtattta tctaatgcac cgttgtcgcg gcgtacgcgg
    26101 atttcagaag catcaacgta ttcaacgaca ccgtcatgtt tacataacaa agcagctcct
    26161 gagtcatgcg ctgatttgta ttccatacca gtaccaaccc atggtgcttg aggattgatc
    26221 aacggcactg cttgacgctg catgttggca cccatcaagg cacggttgga gtcatcgttt
    26281 tccaagaaag gaatacatgc tgtcgcaacg gcaactactt gtttaggtga tacgtccatg
    26341 tagtctactt tatcaataga tacttctaag ttttcacttt gagcacgagc cataacaacg
    26401 tcttgagcaa atgttccatc ttcattcaat gggctgtttg cttgggctac gatgtaatgg
    26461 tcttcgatat cagctgtcaa gtagtcgatt tggtcagtaa cacgacctgt ttcacggtct
    26521 acacgacgat aaggtgtttc gataaatccg aatttattta ctttcgcata actggacaaa
    26581 ctattgatca acccgatgtt tgggccttca ggagtttcga tcggacacat acggccatag
    26641 tgggagtagt gaacgtcacg aacttcataa ccggcgcggt cacgagtcaa tccgccaggt
    26701 cctaaggctg aaagacgacg tttgtgtgtc aattcgccaa gagggtttgt ttgatccatg
    26761 aactgtgaca actgagaaga accaaagaat tctttgatac ttgccacaac tggacggata
    26821 ttgatcaatt gttgaggtgt caatgtttca gtatcttgga tcgacattct ttcacgaacc
    26881 acacgttcca tacgtgctag cccgatacgg aattggtttt gtaaaagttc accgactgaa
    26941 cggatacgac gatttcctaa gtggtcgata tcatcgacat tgccgatacc ttccatcaag
    27001 ttcaagaaat agctcattga agcaatgatg tcggctggac gaaccgtttt tacagctgct
    27061 tctggatagc cgtttccgat cacgtttact tcacgttcag ggtctcttgg tgaaaatact
    27121 ttgatgactt ggacagtcat tggatctgtg accacaccgt cttctgatgg atagtaagtc
    27181 actgaattta ggccattatc aatgaaagga gctagtgttt ccatgacttg gtgagtcaga
    27241 actgttcctt tttcaacgat gatctcgcct gtttctggat caactaatgt ttcagctaat
    27301 gtcagattca atagacgtgt tttcaaatct aattttttgt ttactttgta acgaccaacg
    27361 tttgctaagt cgtaacgttt tggatcgaag aaacgagcat tcagcaagtt acgtgagctg
    27421 tctgctgttt ttggttcgcc tggacgcaaa cgttcgtaaa cgtctttcaa accttcttct
    27481 gttcttgagt cgcttgcgtt tttatgcaag tctttttcga ctgtattgcg taatgtttcg
    27541 ctgtcgccga agatttcaaa gatcgtatca tcggaaccaa atcctaacgc acgaactaat
    27601 acggttaaag gaatcttacg tgtacggtcg atacgtacat aagagatgtc ttttgcatca
    27661 gtttccattt ctagccaagc tccacggtta gggataacag tagaaccgaa tccttctttt
    27721 ccgtttttgt cgacttttcc atggaaatag acacctggtg aacggactaa ctgagaaacg
    27781 attactcgct ctgccccgtt gatgatgaat gttccttgtt ctgtcattaa tgggaaatca
    27841 ccgaagaata cttcttgtgc tttgatttcg ccagtctcgc ggtttgtcaa acgcaatgtt
    27901 acatgtaatg gtgcagagta gtttgcatca tgcgcacgag cttctgctac tgtatacttt
    27961 ggttctttca attcataatc tacaaattcc aaagatagat tgccattgaa gtcatcgatt
    28021 ggcaaaatgt cttcaaacat ttctctaagt ccctcatcta agaaccattg gtaagagtct
    28081 gtttgaattt cgatcaaatt tggtaattcc aacacttcac tgattcttgc gaaacttcta
    28141 cgttcgcgat gttttccgta ttttactacg tgtccagcca agctcttcac ccctctaaaa
    28201 tgtcattcaa aaagactacc cgatcaattg ttacaataat atttcaaata ttatttttag
    28261 gtaacaacag acattttttg cggcttttag gcaaaaaaaa accaaaaata gaacattctc
    28321 ttgaaacctt acttcgtgag cttctacttt tgtttcctta tattaaagcc gaaacggaca
    28381 atatttcgtt tctgcctttt cttcatcaga tagtttttgc aacttcatat actacactta
    28441 ttttctataa aagtcaacac ttcctggcgt ttcttctgct ttttcgcaac attttcttaa
    28501 tgttttcaat tttatgtttt caattatctg acaattctta attgacagac ctttttatct
    28561 cggttatact ttagaaaatt cattttgtgg aggaacttct atgcgaacca acatcaacca
    28621 aatcgcacca acacaactga ataactttta ctttagctac tttagatagg tttgtattca
    28681 tcatgatgga tacaaacgtt aaacgacagt ttgtatctat gatggctaaa gaactttgta
    28741 ttgcagcaaa aagccacgta gacaaaatgc taagtggctg agcagtgaaa atacaaagta
    28801 aaagtatcaa gcattctact ttttcaattt attttccgac ttttcaatcc atttggacat
    28861 tttgtctgaa tggatttttt gtttcccaaa attaaggagg aaaaagaaat gaaaaaaaat
    28921 ataatcatcg gattaggcgc tgccctgcta ctagctttag gaggttgcgc acaaaaagac
    28981 aattccgcag aggcatcaga tggacaaaaa acaattggcg tattgcaact tgtacaacat
    29041 aactcgttag atgctgccta taaaggattc aaagaaggat tagcagaaga agggtataaa
    29101 gaaggcgata atctaacaat cgattatcaa aatgcccaaa acaatcaaga caacctgaaa
    29161 agtatgagtg agaaactcgt caaagaagat cctgatttgc tacttggaat cgccactcca
    29221 gcagcacaat ctttattaaa tgaaacaacc actatcccga tgactgtcac agcggtcaca
    29281 gacttggaac aagcccattt agtcacatcc aatgaaaaac ctggcggcaa tgtgactggc
    29341 acgacagata tggtgccaat cgaaaaacaa atcggcttac tgttaaatat cgtgccagac
    29401 gccaaaacaa tcggcatcat gtataacaac ggtgaagcaa actctaagat ccaagctgat
    29461 ttggcagaaa aagaattgaa aaaagcgggc gtcaaagtca aagtactgac agcaaattcc
    29521 acaaatgatg tccaacaagt gacgaaaagt ctagctaaag atgtagatgg aatctatatt
    29581 cctactgata acacgttcgc aagtgcggca agtgttgtcg gtgaagtcgc aaaagaaaca
    29641 aaaacacctg tcgttgctgg cgcagttgat caagtcaaag acggcggtct agcaacagta
    29701 ggaatcgact acgaaagctt gggtaaacaa acagggatca tggcagcaaa aatcttagac
    29761 ggcaagaatc caagtgaact tcctgtcgaa acagcgaatg atttggaact gtatgtcaat
    29821 aaagacatgg ccaaagcatt aggcatcaat ccagacagca tcaaagaagc tgaataaaac
    29881 acaggaggat tctcatgaca aatttgctct ctcttttctt atcatctgca tcgcaagggg
    29941 tcttatgggc cctacttgcg attggtgtat acgtcacttt ccgtatcttg gacattgccg
    30001 acttgactgc tgaaggaagc tttcctttag gtgcagggat cgcggcagta agtattacaa
    30061 atggctatca tccattgaca gcttgtcttc taggcttttt agggggtgcg gcagcagggc
    30121 ttgtttccgg actgcttcac acaaaattga aaattcccgc ccttcttgca gggatcatca
    30181 ccatgactgg tttatattct gtcacatccc gtgtgatggg tgcgccgaac atctctttat
    30241 tgggtcagcc aacgatcttt cattgggtcg agtcattcgg cataacaaaa gacaacgctg
    30301 tactgatcgt cggtctgctt tttgctttga tcgttgtatg tttacttgtt ttgttcttac
    30361 gtacagaaac aggacttgct attcgtgcaa ctggcgataa tctggcaatg agcgaagcaa
    30421 acgggatcaa cacagataat atgaagatca tcggttatat gatttctaat ggatgcattg
    30481 ctctttctgg ctccttgctt gcgcaaaaca acggctttgc tgatttaaat tctggtattg
    30541 gaacgatcgt aattggactt gcttctatta tcatcgcaga agttttgtta cgtaaccagc
    30601 cactatttct ccgtctcttg actgtcgttt taggtgcggt catttatcgt tttattttag
    30661 cgttagtctt cgaactgaat gttgaaccaa gtgattcgaa acttgcttct gctttcgtgc
    30721 tagtgatctg tctttctttc ccgcagatcc agcaaaaatt ccatttacct caaaaaaaga
    30781 aaggcggtag tgtcaaatga atgacgtctt aacattggaa aatctccatc atacctttga
    30841 aaaaggaacg atcaacgaaa atcatgtcct gaaaggcatt aatctttcct tagatcccgg
    30901 cgacttcgtg acgatcatcg gtggaaatgg ggctggtaaa tctactttgc tgaacagcat
    30961 cgccggtaca ttttctgtcg atcagggtca aattttattg aatggaaaaa atatcacgaa
    31021 aaaatctgtt gtcgaacgtt cgaaatccat cagtcgcgta ttccaagatc ctaaactagg
    31081 gacagccgtt cgtttaacgg tagaagaaaa tcttgctctt gctatgaaac gaggaaaaaa
    31141 acgcggcttc ttccgtggtg tcaaaccaca agaccgaaca tttttcaaag accagctggt
    31201 tcggctaaat ttggggttgg aaaatcgttt atcctcagaa atcggtttgc tgtctggagg
    31261 acaacgtcaa gctattacgt tattgatggc cacattacag cgtccagaac tgatcttatt
    31321 agatgaacac actgcagcgc ttgaccctgc tacttccaaa acggtgatgg aattgacaga
    31381 aaagctgatc actgagcaga atctcactgc ttttatggtc actcatgaca tggaagacgc
    31441 gatccgctat gggagtcgac tgatcatgct gcatcaagga aggatcgtcg tagatatcca
    31501 gcaagaggaa aaacagcagc tcacagtgcc ccaattatta gcgttgttca aacaaaacag
    31561 cggcacggca ttaaaagacg atcaagtact tttatcttca taatcagaaa gaggatggga
    31621 caaaaaatga acgcccaccc tcttcttttt tctctttcct tctttcgttt ttgctttatg
    31681 tatcaaccgt tgtttcttcg aaaactattt tgttctctcc ttcttcctga tagaatggat
    31741 gggaaaggag cttgatgcca tgacaacgaa agacaaggta cttcaattat tgaaaaagac
    31801 accagatttt ctttccggag aaaagttggc acagcaactg gaagtttccc ggacaagtat
    31861 ttggaaagcc atcaaagaat tggaaaaggc aggctatcgt tttgagcacg ggcctacagg
    31921 gtatcgttac ctgccctctg acgtactaga tgcaaaagaa atccagcaag agctacggtc
    31981 gtttattcct gatttgaaag tacaaacgat gttgacttct gaatctacga tgaaagatgc
    32041 caaactggca gcagctcaag gagattctgt ttcgaagtta tttgttgctg atacacaaga
    32101 acaagctcgt ggacgttttg gacggccttt tttcgctgaa cctggacgag gaatctacat
    32161 gagtctcttg ctccatccag acaaacggtt tgatgaattg cctcaatata cgatcatcgc
    32221 agccagtgcc tgtgtgaaag ccattgaatc tgtaaccggt cgttctgctt cgatcaaatg
    32281 ggtcaatgat atctacatgg atggaaaaaa agtctgtggg attttatctg aagcagtcag
    32341 tgatatggaa agcggcagga tcagtcatat catcattggg atcgggctta atttttctgt
    32401 tcctcaaaat cattttccag ctgatctcca agaaaaagcc atctctgtct ttcccgacgg
    32461 acaagctagt gtcacaagga atcagctaat cagcaaaatc tggcaagaat tgttcactta
    32521 tctttctcac ccagaaaaag cgttggactt ctataaagaa aaatcattcg tattgggaaa
    32581 aaccgtgtcg ttcaatcaaa aaggaacgac ttatgtcggt cttgcaaaag aaatcggtga
    32641 tcatggcgaa ctgatcgtcc aaacaaatga acagaagatg agtttgtctt ctggcgaaat
    32701 cagtctcaca tctatccgta cgcaataatt atcagatttc acaaaaggac acctgatctc
    32761 atacagtgtc cttttttctg aattctcttc aaaagactct ccttattcta tcagctgaca
    32821 gtttctctat cctctagaaa aaggtattct aaaaatatct ataaagcatg gtacactttc
    32881 tttaaatgaa tcaatgcaaa aaacaagaga gtcatcagtt tacacgatca aaagagatag
    32941 caggccgctt cctttttcaa tcttatagat agaccatggg taacgataaa aacaaaaaag
    33001 aacttgcgac ttattttgcc gtaagttctt ttttagctag tgccttaatc tcttaatccc
    33061 aacaaacgtc tcgcatagat cacctgacca gagtgcatag cagcatcatc aataatagaa
    33121 acgagacgca ttcctcgtgt gactggtggt gtccagttct cgtcaattac ttcatctagc
    33181 tcggaaggtt ccagccttct taagtaagaa atagcaaaat cagcagctcc tcgcaagtat
    33241 cctagcagat cttctatttg attagcttgg atctgctttg cttgttccag actatgcttc
    33301 caatcttgtt cgttatctgc tatcgtcatc ggaaaccggc tttcccagtc ttgactatgc
    33361 caaattggag aagttcctgc taaatcagca atctggaaat ctaattctcg agcggtatgc
    33421 caagccagcc aagtcattga tttgatctgc ggggctctat cttccactgg aaaacgattg
    33481 gcctcctcaa tagagacgcc ttcaaacaaa cgttcaaacc gatcgtatgc ccgatccaca
    33541 ctctcgcata gcatatctaa ataatccatt tattttcctc ctttttattt atcctactta
    33601 ctttttccta attctaggat acctacttat atgttactac acacccttat tttttacact
    33661 ttaacagtca ccatcatcaa tagtttggag attgaaagaa aaagatagtg tcgaactatc
    33721 aattgaacaa gaagcaacaa tatgaacgtt tcattaatat tctttaaata aacaatgatt
    33781 tttcaaacga acagtttaag actaggagaa aaagagtata ataggaaaat gtttgaaagg
    33841 aaaggaaggg gaaaaatgaa aaaaagggca agtttactag tcctagttgc tttgttgttc
    33901 aatatctttt cgtatggtgt ggccgtttca gcggaaacat acattcctaa agaccctggg
    33961 aatatttata atgatctaac tggtaactat ggcttgttag gaattgctag tcaattccac
    34021 gtatttgcta aaaataagac aactgtgaat gcacatacag acggaaatat agcaacacat
    34081 gaacttgatg caaaaaataa ttttggtaca gagcttcatg aaggtttatt gaaccaagaa
    34141 ataaattata tacaaaaaac tgattctttc gctaattctt caggcatacc tacaggtagc
    34201 acaagaatga ataaatttgt cgttggcagt aacgtggatg tagcacttca agatggtcat
    34261 cctacagtca atggatccag gcttgatcac ttaactccag atgattttta taaagatcgt
    34321 gctgggcatg aatatattga tttcgagcag gaatttgcta aattgaatgt agcagcaaat
    34381 gatcttgcaa cgatcactcc agctaaaaca tatactgctg cagattttcc agacatgaac
    34441 aatcggacaa tcgatttaac tgatttatca gataccgggt ttttattggt aaatatcgat
    34501 gcagaagtgt taacgatgaa tacgccactt caaatcatta atccaaatga tcaagtagtt
    34561 gtatttaatg tcatcaacag ttcttctgca ttgaacgtac agtcaccaat caaatacaat
    34621 gatcgttcaa atcatgaaac agaagatttt tcggatgcga atatctcttg gaattttgga
    34681 aatgagatga ctgatttgac aatcagtgct ccttttcaag gaacaatcct tgcgcctaat
    34741 gctactatcc gcattactca aaaccaagac ggaacgatta taggaacaaa tgtgatttta
    34801 gatgcagcta ccaatcgttg ggatcctaac gaaatattta tcagcgacaa tggtacagat
    34861 acgacagaca caagcgacag tacagataca tctaccacaa gtgatagctc agattcatca
//
LOCUS       DAHHZJ010000012        34888 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.012, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000012 DAHHZJ010000000
VERSION     DAHHZJ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.012"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="NBL02_000450"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="NBL02_000450"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCD2571163.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="NBL02_000451"
     CDS             complement(937..1518)
                     /locus_tag="NBL02_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCD2571164.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="NBL02_000452"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="NBL02_000452"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCD2571165.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="NBL02_000453"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="NBL02_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCD2571166.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="NBL02_000454"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="NBL02_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCD2571167.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="NBL02_000455"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="NBL02_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCD2571168.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="NBL02_000456"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="NBL02_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCD2571169.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7567..7875
                     /gene="rpsJ"
                     /locus_tag="NBL02_000457"
     CDS             7567..7875
                     /gene="rpsJ"
                     /locus_tag="NBL02_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCD2571170.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7906..8535
                     /gene="rplC"
                     /locus_tag="NBL02_000458"
     CDS             7906..8535
                     /gene="rplC"
                     /locus_tag="NBL02_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCD2571171.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8563..9186
                     /gene="rplD"
                     /locus_tag="NBL02_000459"
     CDS             8563..9186
                     /gene="rplD"
                     /locus_tag="NBL02_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCD2571172.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9186..9476
                     /gene="rplW"
                     /locus_tag="NBL02_000460"
     CDS             9186..9476
                     /gene="rplW"
                     /locus_tag="NBL02_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCD2571173.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9515..10348
                     /gene="rplB"
                     /locus_tag="NBL02_000461"
     CDS             9515..10348
                     /gene="rplB"
                     /locus_tag="NBL02_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCD2571174.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNRAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10391..10669
                     /gene="rpsS"
                     /locus_tag="NBL02_000462"
     CDS             10391..10669
                     /gene="rpsS"
                     /locus_tag="NBL02_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCD2571175.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10692..11039
                     /gene="rplV"
                     /locus_tag="NBL02_000463"
     CDS             10692..11039
                     /gene="rplV"
                     /locus_tag="NBL02_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCD2571176.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11053..11709
                     /gene="rpsC"
                     /locus_tag="NBL02_000464"
     CDS             11053..11709
                     /gene="rpsC"
                     /locus_tag="NBL02_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCD2571177.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11712..12146
                     /gene="rplP"
                     /locus_tag="NBL02_000465"
     CDS             11712..12146
                     /gene="rplP"
                     /locus_tag="NBL02_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCD2571178.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12136..12324
                     /gene="rpmC"
                     /locus_tag="NBL02_000466"
     CDS             12136..12324
                     /gene="rpmC"
                     /locus_tag="NBL02_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCD2571179.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12349..12615
                     /gene="rpsQ"
                     /locus_tag="NBL02_000467"
     CDS             12349..12615
                     /gene="rpsQ"
                     /locus_tag="NBL02_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCD2571180.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12673..13041
                     /gene="rplN"
                     /locus_tag="NBL02_000468"
     CDS             12673..13041
                     /gene="rplN"
                     /locus_tag="NBL02_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCD2571181.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13078..13386
                     /gene="rplX"
                     /locus_tag="NBL02_000469"
     CDS             13078..13386
                     /gene="rplX"
                     /locus_tag="NBL02_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCD2571182.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13414..13953
                     /gene="rplE"
                     /locus_tag="NBL02_000470"
     CDS             13414..13953
                     /gene="rplE"
                     /locus_tag="NBL02_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCD2571183.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13972..14157
                     /locus_tag="NBL02_000471"
     CDS             13972..14157
                     /locus_tag="NBL02_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HCD2571184.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14193..14591
                     /gene="rpsH"
                     /locus_tag="NBL02_000472"
     CDS             14193..14591
                     /gene="rpsH"
                     /locus_tag="NBL02_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCD2571185.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14623..15159
                     /gene="rplF"
                     /locus_tag="NBL02_000473"
     CDS             14623..15159
                     /gene="rplF"
                     /locus_tag="NBL02_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCD2571186.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15320..15676
                     /gene="rplR"
                     /locus_tag="NBL02_000474"
     CDS             15320..15676
                     /gene="rplR"
                     /locus_tag="NBL02_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCD2571187.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15697..16197
                     /gene="rpsE"
                     /locus_tag="NBL02_000475"
     CDS             15697..16197
                     /gene="rpsE"
                     /locus_tag="NBL02_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCD2571188.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16212..16391
                     /gene="rpmD"
                     /locus_tag="NBL02_000476"
     CDS             16212..16391
                     /gene="rpmD"
                     /locus_tag="NBL02_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCD2571189.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16435..16875
                     /gene="rplO"
                     /locus_tag="NBL02_000477"
     CDS             16435..16875
                     /gene="rplO"
                     /locus_tag="NBL02_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCD2571190.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16875..18170
                     /gene="secY"
                     /locus_tag="NBL02_000478"
     CDS             16875..18170
                     /gene="secY"
                     /locus_tag="NBL02_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCD2571191.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18232..18879
                     /locus_tag="NBL02_000479"
     CDS             18232..18879
                     /locus_tag="NBL02_000479"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCD2571192.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19070..19288
                     /gene="infA"
                     /locus_tag="NBL02_000480"
     CDS             19070..19288
                     /gene="infA"
                     /locus_tag="NBL02_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCD2571193.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19321..19437
                     /gene="rpmJ"
                     /locus_tag="NBL02_000481"
     CDS             19321..19437
                     /gene="rpmJ"
                     /locus_tag="NBL02_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCD2571194.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19455..19820
                     /gene="rpsM"
                     /locus_tag="NBL02_000482"
     CDS             19455..19820
                     /gene="rpsM"
                     /locus_tag="NBL02_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCD2571195.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19848..20237
                     /gene="rpsK"
                     /locus_tag="NBL02_000483"
     CDS             19848..20237
                     /gene="rpsK"
                     /locus_tag="NBL02_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCD2571196.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20317..21255
                     /locus_tag="NBL02_000484"
     CDS             20317..21255
                     /locus_tag="NBL02_000484"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCD2571197.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21283..21663
                     /gene="rplQ"
                     /locus_tag="NBL02_000485"
     CDS             21283..21663
                     /gene="rplQ"
                     /locus_tag="NBL02_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCD2571198.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21935..22774
                     /locus_tag="NBL02_000486"
     CDS             21935..22774
                     /locus_tag="NBL02_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCD2571199.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22750..23619
                     /locus_tag="NBL02_000487"
     CDS             22750..23619
                     /locus_tag="NBL02_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCD2571200.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23609..24406
                     /locus_tag="NBL02_000488"
     CDS             23609..24406
                     /locus_tag="NBL02_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCD2571201.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24609..25355
                     /gene="truA"
                     /locus_tag="NBL02_000489"
     CDS             24609..25355
                     /gene="truA"
                     /locus_tag="NBL02_000489"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCD2571202.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(25810..28479)
                     /locus_tag="NBL02_000490"
     CDS             complement(25810..28479)
                     /locus_tag="NBL02_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HCD2571203.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(28717..28905)
                     /locus_tag="NBL02_000491"
     CDS             complement(28717..28905)
                     /locus_tag="NBL02_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCD2571204.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            29175..29537
                     /locus_tag="NBL02_000492"
     CDS             29175..29537
                     /locus_tag="NBL02_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCD2571205.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            29589..31271
                     /locus_tag="NBL02_000493"
     CDS             29589..31271
                     /locus_tag="NBL02_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HCD2571206.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            31388..33424
                     /gene="recG"
                     /locus_tag="NBL02_000494"
     CDS             31388..33424
                     /gene="recG"
                     /locus_tag="NBL02_000494"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCD2571207.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            33473..34474
                     /gene="plsX"
                     /locus_tag="NBL02_000495"
     CDS             33473..34474
                     /gene="plsX"
                     /locus_tag="NBL02_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCD2571208.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            34596..34838
                     /gene="acpP"
                     /locus_tag="NBL02_000496"
     CDS             34596..34838
                     /gene="acpP"
                     /locus_tag="NBL02_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCD2571209.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT        11388 a         6406 c         7511 g         9583 t
ORIGIN      
        1 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
     1861 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttgaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
     7321 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
     7381 gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc
     7441 agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga
     7501 acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg
     7561 aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta
     7621 gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt
     7681 ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac tcataaatac
     7741 aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac
     7801 ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc
     7861 gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc
     7921 ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta
     7981 actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc
     8041 tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg
     8101 aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat
     8161 gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga
     8221 gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc
     8281 cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca
     8341 atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt
     8401 ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt
     8461 atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct
     8521 gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta
     8581 ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt
     8641 gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa
     8701 ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt
     8761 caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt
     8821 ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt
     8881 cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc
     8941 gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta
     9001 tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca
     9061 gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt
     9121 gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt
     9181 gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg
     9241 ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa
     9301 aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac
     9361 gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga
     9421 tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac
     9481 taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac
     9541 ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc
     9601 tgaaaaatca ttgttacaac cattgaaaaa ccgtgctgga cgtaacaaca acggccgcat
     9661 cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg
     9721 taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc
     9781 taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg
     9841 actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc
     9901 tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg
     9961 taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg
    10021 caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg
    10081 tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg
    10141 acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga
    10201 tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc
    10261 ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact
    10321 tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag
    10381 gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa
    10441 aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg
    10501 ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca
    10561 tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac
    10621 aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag
    10681 gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca
    10741 cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg
    10801 caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga
    10861 tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa
    10921 gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat
    10981 cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag
    11041 gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc
    11101 gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta
    11161 agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc
    11221 gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt
    11281 ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa
    11341 agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt
    11401 gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc
    11461 atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta
    11521 aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact
    11581 ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga
    11641 gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga
    11701 gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa
    11761 tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg
    11821 ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt
    11881 acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca
    11941 aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag
    12001 taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag
    12061 cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa
    12121 tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt
    12181 gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt
    12241 caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact
    12301 gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga
    12361 agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt
    12421 accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct
    12481 aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt
    12541 atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa
    12601 gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg
    12661 aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca
    12721 cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt
    12781 gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac
    12841 gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat
    12901 attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca
    12961 cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca
    13021 ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg
    13081 tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc
    13141 gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt
    13201 aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg
    13261 ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc
    13321 tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat
    13381 aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt
    13441 aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct
    13501 aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac
    13561 ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa
    13621 gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt
    13681 acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca
    13741 cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact
    13801 ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta
    13861 cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg
    13921 ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa
    13981 aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt
    14041 tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc
    14101 ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa
    14161 gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt
    14221 ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat
    14281 caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg
    14341 aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg
    14401 gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca
    14461 aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg
    14521 aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt
    14581 acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat
    14641 aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt
    14701 aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa
    14761 ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga
    14821 acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg
    14881 ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac
    14941 gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca
    15001 gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca
    15061 aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc
    15121 gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag
    15181 aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta
    15241 cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa
    15301 aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag
    15361 acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt
    15421 tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc
    15481 aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc
    15541 tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtttttga
    15601 ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa
    15661 tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat
    15721 ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga
    15781 cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtaggattt
    15841 ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag
    15901 aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct
    15961 tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt
    16021 ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta
    16081 ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa
    16141 cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga
    16201 ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc
    16261 aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta
    16321 agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg
    16381 aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa
    16441 cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt
    16501 acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca
    16561 ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca
    16621 aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg
    16681 aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg
    16741 aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact
    16801 gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca
    16861 atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat
    16921 cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg
    16981 taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt
    17041 tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac
    17101 catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg
    17161 ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc
    17221 tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg
    17281 ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc
    17341 taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg
    17401 gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca
    17461 gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta
    17521 tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc
    17581 aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa
    17641 gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt
    17701 cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa
    17761 actggtacaa agtgatgatg gagatcttta actataatac agcacctggt gctacgattt
    17821 atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga
    17881 aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg
    17941 ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc
    18001 ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa
    18061 tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac
    18121 agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt
    18181 tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc
    18241 attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct
    18301 tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca
    18361 gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta
    18421 acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta
    18481 gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg
    18541 aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt
    18601 ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct
    18661 acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat
    18721 gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc
    18781 ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat
    18841 gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact
    18901 tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac
    18961 ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc
    19021 gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga
    19081 agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt
    19141 tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta
    19201 catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg
    19261 tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc
    19321 atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa
    19381 ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg
    19441 gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag
    19501 tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg
    19561 atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta
    19621 tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga
    19681 acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc
    19741 ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta
    19801 tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa
    19861 aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt
    19921 ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt
    19981 catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa
    20041 tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa
    20101 cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt
    20161 tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa
    20221 aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac
    20281 aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga
    20341 atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga
    20401 gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt
    20461 gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt
    20521 gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat
    20581 ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc
    20641 gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc
    20701 tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa
    20761 gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac
    20821 acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat
    20881 tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg
    20941 agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa
    21001 gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa
    21061 atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga
    21121 atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac
    21181 ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta
    21241 cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta
    21301 ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc
    21361 aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa
    21421 atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta
    21481 cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta
    21541 caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc
    21601 attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc
    21661 taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga
    21721 agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa
    21781 gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga
    21841 aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg
    21901 ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa
    21961 gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac
    22021 aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact
    22081 tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca
    22141 aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatgg tttttcaaaa
    22201 tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa
    22261 tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag
    22321 aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt
    22381 tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag
    22441 tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga
    22501 aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg
    22561 gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc
    22621 tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag
    22681 cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt
    22741 ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca
    22801 tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca
    22861 ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt
    22921 aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat
    22981 aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag
    23041 ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc
    23101 gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc
    23161 tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct
    23221 ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat
    23281 ccccagggca gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc
    23341 actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat
    23401 gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt
    23461 gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta
    23521 atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct
    23581 atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat
    23641 gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta
    23701 ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt
    23761 cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg
    23821 cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg
    23881 agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct
    23941 ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac
    24001 accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg
    24061 gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt
    24121 gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga
    24181 aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag
    24241 cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg
    24301 ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat
    24361 tgcttttgta ttattaactg tgctactgat ctttttaaga ggataaacgt tcagtgggcc
    24421 gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcaagcag tgtatgacaa
    24481 gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa
    24541 agaacgaaaa aattttatag aaaaatcatt attccagata accttttagg aacaaaggag
    24601 agaaaaacat ggtacgttac aaagcaatca ttgcgtacga cggaacaaat tttaatggat
    24661 tccaaaaaca gccaaatggg cgtactgttc aagaagaagt agaaaaaaca ttacaaaaaa
    24721 tggcaaatgg aaaagaaatc acggtctttg gttcgggaag gacagatgcc ggagtacatg
    24781 cgatcgggca agtaatccat tttgattatc cagaggagcg tccgttagaa cgaatgcgtt
    24841 tcgcgctaga tacccagtca ccagaggata ttgctgtaag acaagtagaa attgtttcag
    24901 aagacttcca cgcaagatat ttggtcaaag aaaagacgta tcaatttcga gtggatatag
    24961 ggaaaccacg cagtcctttt cgacgccatt atgcaagtta ttttccttac ccacttgatt
    25021 tggaaaaaat tcagcgggcg cttcctgatt tattagggac tcacgatttc acctcttttt
    25081 gtgcatcagg aagctcgata gaggataaaa tacgtacgat ctacgaggct aagatggagg
    25141 taaatgaaac aaaagacgaa ttgttgttta catttagggg aaatggcttc ttgtataaga
    25201 tgatacggat cttagtagga acgcttttga aaattggcaa tggccgattg cctgaagata
    25261 gtatcccaga tattattgcg aaaaaagatc gtaatgcagc aggacctaca gcacaccctg
    25321 aaggacttta tttgtatgaa gtgaaatatg aataagaggt tatgatagaa gtggtcagct
    25381 tcaagaaata agaaggtatt tacgaaaatt acttctcaaa attttgcaaa atttcggctt
    25441 attttcgaag aagctacttc tggaacaccg tttaaggaag aggtcgtgaa atcagcgttt
    25501 tgacctgagt attagagaaa caaacgggaa aatagctcct catattttgc cgtttgttga
    25561 gctaataccg aaggtcagct gattgaacac cgtttaagaa agaggtcgtg agcctgtcat
    25621 ttaactctga gtcacaaaga gcaatttcaa aaaacagctt ctcatctttg atgaatatcg
    25681 tgacttgtgg acgaggcgtt gataagtgaa tcatcttttt gttacctatt ttttctgtaa
    25741 ccaaaaaacg atggaacgtt gttttgattg cccttggtgc ggacagtctg aacaggttcc
    25801 atcgtttctt tattttctac gtcttcttct gtgtgtgttt cgtggttgtt cttctattgc
    25861 tggatcaatc gtgccataaa aggggcgatt gcggtcaagg taccagacat aaagaataaa
    25921 caatgcaata cataggacaa aaaggattgc acctatcgta aatccttcag gcaagtaact
    25981 gaaagtgaca gtatgtttac ctgctggaac ttttatcatc aagaatgcgt ctttgaaatt
    26041 gctgaccttg actggtttcc catcaatttt agctttccat cctttgtcat aaggaatagt
    26101 tgtcaccaac atggaatctt cagcggtatt tacagtccca gaagcttttc tgccagtggc
    26161 ttccatttcg acaccttttt ctttgatctg attcattgcg gcttgatagg ctacaacatc
    26221 tagaccaacg actttaggct ccatgaaact tatttctttc gtcccataaa aacttgcagt
    26281 aaatgtcact gttgtctctt gaggataata gccaagatca tagtattgac cagaaatgtt
    26341 gatttgcgtt tttcgcgatg taccattgac agtgatcgtc gcactagaac tttctaattg
    26401 cgcaaaattg gttgggaaca aacttaagta agcttgtgta tttgcaggca catggacttc
    26461 ccaagtgagc tcttttggct ggttatctgc tgtttctgag taagtgacgc cagtcgttgt
    26521 ctttgtgatc gtcgtattca actgattctt caaagtaggt gtatagaatg taaaatactg
    26581 ttcatttaga ccagacaatg tattgatcaa gtttgtctga ctggtcaaat tatctgtcac
    26641 tggctgctca acatcattaa cggacaacgg tgcagtaaat gctagtggca atgcattgac
    26701 attctgatac agtgtatagt cgccgctttg accagcttgg cggaagccat atttactgaa
    26761 cgtgctttcg tttttagaaa tgttatattt gattcctgta aacccatcca tcagcaaagt
    26821 attattgtta taacggatat tcaaatttgt ccctcttgaa cggaaaccta atttatccag
    26881 gtaagaggaa gaattacgat ttcttataga agaaaacaaa ctgataccac tgtaaccata
    26941 gttgatcgaa tcgtttgcag atactggatc cagattttcc aagcggtaaa acgagtcgga
    27001 atcttttttt gtttcattta caagagcttt caagtcagga taaggatcag tatataaact
    27061 gcgggaagca taattccaat catcaagaat gccatggatc atactgttcg tattgaccag
    27121 tgcttcagca gacatgatca ataataacaa tatactcaga tgattgaatg aaagtttttt
    27181 atgctgataa aatcccacag caaaagcata aatccctaag aaaaccactg tcaatacaaa
    27241 ggaagtcatc gtcacatagg tgtagcttcc ttcaggtttg atgccccatg ccaccgcaaa
    27301 gagagcagct acgaccaaaa tgattcctaa caacagccca cgatcgtctt gttctatctt
    27361 ttcccatcct tttcctgcaa gcaggagcac taagaaagag aacagatagg cataacggaa
    27421 taaaaacata ttcggtgcat gcataccatg ccagaacaaa ttcaatggaa caagataaaa
    27481 acttgcgata agtatggcga aaagactgcc gtacaaaaat ttatttttca acggtacttt
    27541 ccgactggca aaataaaaca gacagaggac taatggtagc agacctgcgt aaacgaacgg
    27601 aatcgatccg tattttgtcg tgtcataaac accaatcata tttttcatga ttagatccag
    27661 ataagaagtc gcttctgttt tcaacgtagt gatctgactc agtgcctcgc catttgaccg
    27721 aagatccagc actgctggca atacaatgat cattgaagcg ccgcctgcca gtaaagaagt
    27781 gataccataa ggaacaatcg tttttttata ttctgaccag tttgtcaata gacgggcgat
    27841 aaaatataag aaagaaaata cgccaatcat aaaacccata taaaaactgg ataaaaacaa
    27901 gagtaaataa ctgataaata aaagtttcgg ccgtttaaga tccataacac gatgaatccc
    27961 caaaatcact aatggaagat aaaaaaatgc atccagccac atgatgatct ccgaatgtgc
    28021 agtgataaat gagatcaatg cgtaactgac gctaagtgca acatgccccc agcgaggaat
    28081 cttgaacgta cgtgcaagaa accaaaaagc caatccggca cagccgacct taagcaaagt
    28141 cagaacatac agtgcatctg gcatcatctg gtttggaaaa aagaacacca gtggcgtgaa
    28201 gatcccgcct agatagtaag aaattaaaga taaataattc aatcctagag aagcattcca
    28261 tgtataaaaa atactttgct ttccaagaag aacatttcgg aagctggcat gaaaattaga
    28321 aaactgtgaa aaggcatcac ttgccaaaat acttctgctg cttccaggat agattccgat
    28381 acttagataa acaagtgcta aaatcaaaaa cggaatcagg aaactagctg ctgcgtagcg
    28441 tccgttttct ctaaagaatg actttaaatt ttttttcata tttattttcc accaaatttc
    28501 tatgaagttg tattcagttt atcatatcct tgtttttctc tgcttaaaaa gagtctagtt
    28561 ttcacaaaaa aataaaattt agaataaata acttcataat ttgttcgtat aacaaaaata
    28621 aacgcaaaaa aagggtagga atcaaaagtc ccacccttaa aaatttttat taaataaaaa
    28681 accgttcact atctgaacgg tccaagtatc attcaattaa acgcgttcaa cttttcctga
    28741 tttcaaagca cgagttgaca cccaaacttt tttaggtttt ccgtcgacca taacgcgaac
    28801 tttttgtaag ttaggtttaa cagtacgttt tgttgagttc atagcgtgtg aacggttgtt
    28861 tccacttttt gtcttgcgac ctgtaaagta acaaactttt gccatggtaa tttcctcctt
    28921 tgccttgatt tggagcaata gaataattca gctcatacca gactaacata tcatatagaa
    28981 tgggaatttg caagtacatt ttcatagata caattgacaa ttgagaagaa taattgtaaa
    29041 ttaaacaagg ttatacctga aattttgcaa ggtttttgag tactttgggt aactgccttt
    29101 tatttcaagt tgcgctatga taaaatatga tagaagaaaa atgggagttt tttacccgtt
    29161 aaggaggctc attcatggct gtaaaaattc aaacgtcagc tggaaccatt gaaattacca
    29221 atgatgtaat tgcaacagtt gttggtggcg ccgcgacaga tgtgttcggt gttgtaggaa
    29281 tggccagcaa aaatcaaatt aaagataata ttactgagat tttgcgtaaa gaaaattact
    29341 caagaggagt agttgtccgc caagaagata atggtattgc tgtagatgtt tatacgatta
    29401 tcagctatgg tacgaaaatc tctgaagttt cccgcaacgt ccaagaaaaa gtgaaataca
    29461 atctagaggc attattaggc gtaactgcca attcagttaa tgtctttgta caaggtgttc
    29521 gtgtactgcc agactaggca ggagactggc tggtaactca gcagtcgatc tgaaggagga
    29581 ttatctaggt gagtgtaaca gaaataagtg caagccagtt ccaggaaatg gtccaggcag
    29641 gtgcgaatcg tctgcaagta aatgcagaat acgtcaattc attaaatgta ttccctgtgc
    29701 cagatggcga tacgggaaca aatatgaatc tatctatgac tagtggagct aaggcagtag
    29761 tagattcaac ctcagaaaaa gtaggagaat tagcagcaat gctttcaaaa ggattgctga
    29821 tgggtgcgcg cggaaattcc ggcgttattt tgtctcaatt gtttcgtgga ttttctaagc
    29881 aaattccaga tgttgtctca ttaaatgctt ctgatcttgc tgcagccttc actcatggag
    29941 ttgaaactgc gtataaagca gtaatgaaac cagtagaagg aacgattttg acagtagcta
    30001 gagaagcagc gaaagctgga gaaaagaaag caaaaagtac tgatgatgtc gttgaagtaa
    30061 tgacagctgt tgtaaaagga gcaaagcgtg ctttagctaa aactccagac ttgcttccag
    30121 tcctaaaaga agtcggtgtc gtagacagtg gagggcaagg actgctgttt gtttatgagg
    30181 gctttttaag tgcattgaat ggagaattcc aagcggatga aacatatgaa ccttctccag
    30241 cagaaatgga tgatatggtc aatgcagaac atcatcgcag tgtccaaggt caaatggcga
    30301 cagaagatat ccattttggt tactgtacag aaatcatggt aaaaatcgga gaaggaccta
    30361 cagtcgatag tacattcgat tacgaagaat tccgtaacta tttagatggt ataggagatt
    30421 ctttacttgt ggtcaatgat gacgagatca tcaaagtcca cgtgcataca gaaaacccag
    30481 gagaagtctt gaattatgga caaaaattcg gttcattagt caaaatcaaa gtcgacaaca
    30541 tgcgcttgca gcatgagacg attttggaac acgatgaaga agtgaacgag tttgcgaaaa
    30601 aagaaatacc acgtatcccg tatggtatcg tagcgatcgc tgctggtaaa ggcgtccaag
    30661 agctgtttga aagtttaggc gctaattatg ttatcagtgg tggacaaaca atgaatccaa
    30721 gtacggaaga cattgttaaa gcgattcaag aagtaaacgc agataaagtg attgtcttgc
    30781 caaacaataa gaacatcttc atggcagctg atcaagcagc agaagtagca gatatcccag
    30841 tagcagttgt tccttcaaga acgatttccc aaggattgac agctatgctt tcattcaatg
    30901 atcaagcttc tttagaagaa aacaaagaaa cgatgacaga aatgttgtct agcgttgtga
    30961 gcggtcaagt cactcacgcg atcagagata cctcaattga cggtgtagag atcaaagaag
    31021 gagacttttt gggcatgatc gatggaaaaa tcctgatctc taacccagat atcacagcaa
    31081 cagcgttaag tacactagaa caaatgatca atgaggacac agagatcgta acgatcttgg
    31141 caggtgatga aggaacagtt gaacaagcaa atgaatttgc tgagaaacta acagaaaaac
    31201 atcctgattt ggaaatcgag atccaccaag gagatcagcc tgtctatcct tatctatttt
    31261 ctgctgaata atttctgtgt cagaatatct ttctaacaca gtattcattc ataaaaggcc
    31321 ttcggttagt gatcaggtat gatcgctgac caagtccttt ttttgttaaa ggagggagga
    31381 aaaatttatg cgttctttag atgatccaat cactatgctg gcaggagtcg gagaaaaaag
    31441 agcagctagt cttgccagct tgggcatcca aacgattgaa gacttactga cttattatcc
    31501 ttttcgctac gaagatattc aggaaagaaa tctaaacgag atccaggatc aagaaaaggt
    31561 tactttgaaa ggaatcgtag tctcgcctcc tgtgatgaat cgatttggtt ataaaaaaag
    31621 ccgattgcaa tttcgtatga tgcaagaaca tgatgtgttc aacgtttctt tttttaatca
    31681 accttatcta aaagaaaaag tcattttatc ggaggaaatc gccgtatacg gtaaatggga
    31741 tgcaaagcgt aaagccttga acgggatgaa gattttaggt tctcaaacaa tggatgactt
    31801 ttcgccgata tatcacgtca acaaatcaat tcgtcaaacg acattagttg atctaatcaa
    31861 aaaagggttt caagaatacg gcacattgat tgaagaaaat ctgccaaaca accttgtgga
    31921 gaaatatcga ctgcttgacc gagcttctgc tgtaagaagt atgcattttc caaaagacca
    31981 cgaagaaaac catcaagcca aacgacgtgt cgtttttgaa gagttctttt tatttcaaat
    32041 gcgtcttcaa ggattgaaaa aagcagaaaa agcagaaacg aatggtttgg agatccttta
    32101 tgatgtccaa aaattgaaac aatttactca aaagctacct tttgaactga caaatgcaca
    32161 aaaacgtgta acgaatgaaa tctgtcggga tcttcgcagt ccgcagcata tgcagcgact
    32221 tttgcaggga gatgtaggta gcggaaaaac agtggttgcc gcgattgcgt tatacgctac
    32281 cgttacagca ggtttccaag gtgcgttgat ggtgcctaca gaaatcttgg cacaacaaca
    32341 tatggaaagc ttgcaacagc tgtttgatcc caatgaagta accatcgcat tgattacggg
    32401 gtcaacgaaa gcaaaagaac gacgtgagct ccttgaaaaa ctagagcaag gggaaattaa
    32461 cgtcgtgatc ggcacacatg cattgatcca agatggagtc gaatttcaga atttaggtct
    32521 agttattacg gatgagcagc accgttttgg cgtgaaccag cggaaagtac ttcgtgaaaa
    32581 aggctggcga ccagatgtct tatttatgac agcgactccg attcctagaa cattagctat
    32641 tacagcatat ggtgagatgg atgtatctat cattgatgaa cttccagctg gacgtatccc
    32701 gatcgaaaca cgctgggtaa gaacacctca attggacagt gtactggatt ggacgaaaaa
    32761 agaactggca agaggacatc aaatgtatgt catctgtccg ctgattgaag aatcagaagc
    32821 attagatgta aaaaatgcag tggaaatcta tgaaaaactt cgtgaactat ttgcaccaaa
    32881 atatgaagtt ggcttgttac atggtaaaat gaagaatcag gaaaaagaag taatcatgga
    32941 agccttcaaa gacaaccaga tgcagatatt agtttcgact actgtaattg aagtaggggt
    33001 aaacgtgccg aacgcgaccg tcatgctgat catggatgcg gatcgatttg gattggcaca
    33061 gctgcaccaa cttcgtggac gtgttggacg cggaagtgat gcttcctact gtatcttagt
    33121 tgctaatcct aaaaatgaac taggtgtgga acggatgaaa atcatgacag agaccaccaa
    33181 tggttttgta ctaagcgaaa aagatctgga attacgtggt cctggtgaag tatttggctt
    33241 ccgtcaatct ggtctgcctc aatttgcgat tgctgacttg gtgacggatg gaaatgtttt
    33301 ggaggtagcc agagaagagg ctgcagccct ttggcaaata aaggattggc agatgcttcc
    33361 tgagtatcag cctttagcgc aaagcctcca aatagaaaat caggataatc gttatttcga
    33421 ttagcagact gtgctatact ttatactgat gacggaaaaa ggagtggaaa caatgaaaat
    33481 tgccgtagat gcaatgggcg gagatcacgc acctcaagca attgtagaag gcgtggcttt
    33541 agcgcagcaa gattttccag aagtagaatt cttactttac ggaaaagaag cagaaatcaa
    33601 gaaatactta acaaatgaaa aaaacatcac gattatccat acagacgaaa aaatcaatag
    33661 tgatgatgag ccagtaaaag caattcgacg aaaaaagact gcatcaatgg tccttgctgc
    33721 acaagcagtg aaaaacggtg aagcagatgc tgtattttca gcagggaata caggcgcgct
    33781 actagcagca gggttgttca tcgtaggacg tatcaaaaat atcgaacgcc ctggcttgat
    33841 gtctaccttg ccagtagtag ggaaagaagg tgccggtttc gatatgctgg atcttggcgc
    33901 taacgcagaa aacaaaccag aacatttgtt gcaatacggt attttaggtt ctttttatgc
    33961 acgtaaagtc cgaggtatcg gacgtcctcg tgtcgcactc ttaaacaacg gaacagaagc
    34021 aacaaaaggt agtgaagtga cgaaaaaagc ctatgaactc cttcagaatg aaacaagctt
    34081 gaattttatt ggaaacgtag aagctcgtga gctactcaat ggtgtggctg atgtagtggt
    34141 cacagatgga tttacaggca acgctgtttt gaaatctatt gaaggaacag caatgaatat
    34201 gatgaatcta ttaaaatctg ctattctaaa tgaagggatc aaaggaaaaa tgggcgctat
    34261 gcttttaaaa gacggcttac gttctttgaa agcagaaatg gattattcca aacatggcgg
    34321 tgccgttttg tttggcttga aagcaccagt cattaaaact catggagcaa caggccccga
    34381 tgctgttcgc tatacgatcc gccaaatcca cacaatgctt gagacagatg tagttggaca
    34441 attagtcgaa caatttgaaa aaagcgaaga atagtatttg gatatctgaa gcgatccgtt
    34501 ccattgaaaa aaaggtggac attcctgaaa aatgccagta aaattagcca tggtgtaggt
    34561 aattgacaag tgtatgattc ttggaggtga aaaagttgac tcgcgaagaa gtatttaaca
    34621 aagtagcgaa aattatttca aatcattttg agatagatac cgataaagtg acggatgaat
    34681 tgaacatcaa agatgattta aaagctgatt caattagtat tatggaattc gttttagagc
    34741 ttgaagatga attcggtacg gaaatttcag atgaagacgc tgaacaaatt gaaacagtcg
    34801 gtggcgcagt agattatatc agcagccatc ttaaataatt tcaaataaaa gcaaagaagg
    34861 ataaaaagct gcatgcagct ttttatcc
//
LOCUS       DAHHZJ010000013        34085 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.013, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000013 DAHHZJ010000000
VERSION     DAHHZJ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.013"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..127
                     /locus_tag="NBL02_000497"
     CDS             <1..127
                     /locus_tag="NBL02_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2969 family protein"
                     /protein_id="HCD2571210.1"
                     /translation="GEILAYGPKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            215..295
                     /locus_tag="NBL02_000498"
     tRNA            215..295
                     /locus_tag="NBL02_000498"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:249..251,aa:Tyr,seq:gta)
     gene            302..373
                     /locus_tag="NBL02_000499"
     tRNA            302..373
                     /locus_tag="NBL02_000499"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:334..336,aa:Gln,seq:ttg)
     gene            498..668
                     /locus_tag="NBL02_000500"
     CDS             498..668
                     /locus_tag="NBL02_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571211.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            888..1595
                     /locus_tag="NBL02_000501"
     CDS             888..1595
                     /locus_tag="NBL02_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCD2571212.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(1655..2299)
                     /locus_tag="NBL02_000502"
     CDS             complement(1655..2299)
                     /locus_tag="NBL02_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HCD2571213.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            2426..2713
                     /locus_tag="NBL02_000503"
     CDS             2426..2713
                     /locus_tag="NBL02_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571214.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            2821..3141
                     /locus_tag="NBL02_000504"
     CDS             2821..3141
                     /locus_tag="NBL02_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCD2571215.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            3222..4535
                     /locus_tag="NBL02_000505"
     CDS             3222..4535
                     /locus_tag="NBL02_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCD2571216.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATIQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            4532..5251
                     /locus_tag="NBL02_000506"
     CDS             4532..5251
                     /locus_tag="NBL02_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HCD2571217.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            5379..5930
                     /gene="raiA"
                     /locus_tag="NBL02_000507"
     CDS             5379..5930
                     /gene="raiA"
                     /locus_tag="NBL02_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCD2571218.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            6152..8686
                     /gene="secA"
                     /locus_tag="NBL02_000508"
     CDS             6152..8686
                     /gene="secA"
                     /locus_tag="NBL02_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCD2571219.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            8812..9913
                     /gene="prfB"
                     /locus_tag="NBL02_000509"
     CDS             join(8812..8883,8885..9913)
                     /gene="prfB"
                     /locus_tag="NBL02_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCD2571220.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            10018..10704
                     /gene="ftsE"
                     /locus_tag="NBL02_000510"
     CDS             10018..10704
                     /gene="ftsE"
                     /locus_tag="NBL02_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCD2571221.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            10688..11581
                     /locus_tag="NBL02_000511"
     CDS             10688..11581
                     /locus_tag="NBL02_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2571222.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            11733..13181
                     /gene="cls"
                     /locus_tag="NBL02_000512"
     CDS             11733..13181
                     /gene="cls"
                     /locus_tag="NBL02_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCD2571223.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            13518..14375
                     /locus_tag="NBL02_000513"
     CDS             13518..14375
                     /locus_tag="NBL02_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCD2571224.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            14490..15410
                     /gene="pstC"
                     /locus_tag="NBL02_000514"
     CDS             14490..15410
                     /gene="pstC"
                     /locus_tag="NBL02_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HCD2571225.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            15410..16294
                     /gene="pstA"
                     /locus_tag="NBL02_000515"
     CDS             15410..16294
                     /gene="pstA"
                     /locus_tag="NBL02_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCD2571226.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            16309..17115
                     /gene="pstB"
                     /locus_tag="NBL02_000516"
     CDS             16309..17115
                     /gene="pstB"
                     /locus_tag="NBL02_000516"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HCD2571227.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            17131..17889
                     /locus_tag="NBL02_000517"
     CDS             17131..17889
                     /locus_tag="NBL02_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HCD2571228.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            17902..18579
                     /gene="phoU"
                     /locus_tag="NBL02_000518"
     CDS             17902..18579
                     /gene="phoU"
                     /locus_tag="NBL02_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCD2571229.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            18810..19847
                     /locus_tag="NBL02_000519"
     CDS             18810..19847
                     /locus_tag="NBL02_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCD2571230.1"
                     /translation="MKNAKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKE
                     DHQLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKND
                     GWLFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILF
                     VVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLT
                     RPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIG
                     SMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISV
                     LFFLTVRRLKR"
     gene            19844..20611
                     /locus_tag="NBL02_000520"
     CDS             19844..20611
                     /locus_tag="NBL02_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCD2571231.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            20711..22279
                     /gene="liaX"
                     /locus_tag="NBL02_000521"
     CDS             20711..22279
                     /gene="liaX"
                     /locus_tag="NBL02_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HCD2571232.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            22576..22896
                     /locus_tag="NBL02_000522"
     CDS             22576..22896
                     /locus_tag="NBL02_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HCD2571233.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            22896..23246
                     /locus_tag="NBL02_000523"
     CDS             22896..23246
                     /locus_tag="NBL02_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCD2571234.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            23413..24351
                     /locus_tag="NBL02_000524"
     CDS             23413..24351
                     /locus_tag="NBL02_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HCD2571235.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            24366..25196
                     /locus_tag="NBL02_000525"
     CDS             24366..25196
                     /locus_tag="NBL02_000525"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCD2571236.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            25240..26265
                     /locus_tag="NBL02_000526"
     CDS             25240..26265
                     /locus_tag="NBL02_000526"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCD2571237.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHHASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            26280..27218
                     /gene="galU"
                     /locus_tag="NBL02_000527"
     CDS             26280..27218
                     /gene="galU"
                     /locus_tag="NBL02_000527"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCD2571238.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            27343..27783
                     /locus_tag="NBL02_000528"
     CDS             27343..27783
                     /locus_tag="NBL02_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HCD2571239.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            27786..28388
                     /locus_tag="NBL02_000529"
     CDS             27786..28388
                     /locus_tag="NBL02_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCD2571240.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(28451..29554)
                     /locus_tag="NBL02_000530"
     CDS             complement(28451..29554)
                     /locus_tag="NBL02_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCD2571241.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            29764..30765
                     /gene="ccpA"
                     /locus_tag="NBL02_000531"
     CDS             29764..30765
                     /gene="ccpA"
                     /locus_tag="NBL02_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HCD2571242.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            31120..31515
                     /locus_tag="NBL02_000532"
     CDS             31120..31515
                     /locus_tag="NBL02_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571243.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            31515..31766
                     /locus_tag="NBL02_000533"
     CDS             31515..31766
                     /locus_tag="NBL02_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571244.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            31809..32204
                     /locus_tag="NBL02_000534"
     CDS             31809..32204
                     /locus_tag="NBL02_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571245.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            32201..33457
                     /locus_tag="NBL02_000535"
     CDS             32201..33457
                     /locus_tag="NBL02_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HCD2571246.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGQLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            33469..33792
                     /locus_tag="NBL02_000536"
     CDS             33469..33792
                     /locus_tag="NBL02_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571247.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            33785..34051
                     /locus_tag="NBL02_000537"
     CDS             33785..34051
                     /locus_tag="NBL02_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571248.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT        11424 a         5861 c         7333 g         9467 t
ORIGIN      
        1 tggtgagatc ttagcctatg ggccaaaaga atatgaaatt ttctttggtg aagaagattt
       61 tggaaaagaa aaaagtcttg aaaatgcgat agaaacagtg attcgacact ggaatctgca
      121 cgaataaaaa aatatcaaaa aaactttcaa taaagggttg caaaaatctg gaaaattagg
      181 tattataact aagtcaggtt gatacaaacc tttcggagga gtagcgaagt ggctaaacgc
      241 gacggactgt aaatccgttc cttagggttc agtggttcga atccactctc ctccatttca
      301 ttggggtata gccaagcggt aaggcaaggg actttgactc cctcatgcgt tggttcgaat
      361 ccagctaccc cagtatacca caatcgtaag ctctcgttag ttcgagagct cttttttttg
      421 ctttgctacc ttgttccttg aagattctgc tgatttaaga tagagttaag ttaactacaa
      481 gaattggagg gatccacatg aaatggaaag aaaaagtaga cgaagcagct gaaaagttat
      541 atgatttggt caaatcagaa aaatacaata ttgaagtgaa tattccgaaa aaaggcggaa
      601 aagcggtcca ggtaaaatca aaacgcccaa cgaatcatac aaaaaaatgg atggctaaaa
      661 accgctaata ataacaaaag atccataagt cggcattttc atcgatttta gggtcttttg
      721 ttattttcta ggttgaacat atctaaaaaa ttgtttctga caataggagt aggaagaacg
      781 atgtgtgtct aaatcctctg ttgatggata gaaaccttta tctttttcag ttgtgaatca
      841 ggtatatacc agttataata gaagaaacga aaggacgggt gtacgttatg tcgaatcaat
      901 tacctgtata tatacagatt catgaccaat taaaaaatga aatcgaacaa ggtatatgga
      961 aagtcggaga ccgtcttcct tcagaaagag aattagcagt gaaattcaat gtcagccgta
     1021 tgacgttgag acaagcaatt caaaacttgg cagatgaagg aatcttagaa agaaagatcg
     1081 gttcgggtac atatgttgcg cgagaaaaag tacaagaaaa aatgtctggt gcaacaagtt
     1141 ttacagaaat tatggaatcg caaaatcgag taccgtctag tcggacaatc tcttatttct
     1201 tgacttcgcc aagctcaagt gaaatggaaa aactaaactt gagtaaagat gacacgatct
     1261 tgcgaatgga acgtattcgt ttcgcagatg atgtccctat ttgttttgaa gttgccagtg
     1321 tgccacaaaa gctgatcgat ggctacagca aagcagagat tactgaatcg ttgtatcgca
     1381 cgctagaaga aaaaggcggg aaaaagatcg gtccagcgaa tcaaacgatt actgctgtgt
     1441 tggcttctga gaaaattgct gactatttag aggtaaaacg aggagatgca gttcttcgtt
     1501 tgcgccagat ttcttatttt gaagacggta cgccgtttga gtatgtgcgt acgcaatacg
     1561 taggaaatcg ttttgagttt tatttggaaa aataaaaaac aggtgatccc tgattcctta
     1621 cttagaaata aaagagaatc agaatcacct gtttttatat atgatcagtt accagttgct
     1681 cgtgataaga aacaggaccc tcttcaaatt ggacttgtcc gttcagcagc tcaatgatgg
     1741 attctttaaa gtggtcagca cttttttcat ctacagcaca gatgacgcta acttgatcag
     1801 tatattggat ttcttttata aaaaaatgtt ggagttcaag ataattctgt aattttccta
     1861 gttgcggata cgtgatcatc acagagatct cctgttgcaa ggtaccttct acgattccaa
     1921 tgtcagacaa tgcttgagag acagcatgag tgtatgctcg tattaatccg cctgtgccga
     1981 gtttcgttcc tccaaaatat cgagtgacta ctgcagctac attgatcagt tgattttttt
     2041 tgaggacttc caacatcggt acgccagccg ttccacttgg ttctccatca tcgctgcttc
     2101 gctggatttc atttttttct ccaatgatga aggcgctgca gttatggttc gctttccaat
     2161 gttctttttt tatttcttga atgaaacttt ttgcttggtc ctctgattct attcgtctaa
     2221 gagaacagat aaagcgtgat tttttgatct caatctcact ttgtccattt tcttttattg
     2281 tacgatagcg ctcgatcatc ttgtcacctc tttttttcat tttctttatt cagtataaat
     2341 aaagttctag gttgtttcaa cactgtaacg gttgcgcaat cgctgtagta tgcgataata
     2401 aagacaaagg taggtggatc tggggatgca ggaacgaatg aaaaagtacg atgcaattac
     2461 gcattatttg aagaacaatg gcggctctca agtaacgttg acctttaccc aatttgacga
     2521 actgctgttt cccagtaacg gacttcccaa aacagcaagg gaaagtacag attggtgggc
     2581 aaatgattat aaacaccctg aaaaaggtgc ctatggctgg ataaatgcag gctatgaagt
     2641 agttgtcatc aatttggaca aagaatatgt cgtgttcaat aaattagtga aatctagttg
     2701 gctatttgat taaataaacc gcgtttagaa aacgttgaca aatttctttc tttttatata
     2761 atagagtaac agacgcgaag aaaacgtttg cgtagtttgt tttaaaaagg agagaaaagt
     2821 atggctaaaa aaacaatcat gcttgtatgt tcggctggta tgagcacaag tttactagta
     2881 acaaaaatgc aaaaagctgc tgaagcaaaa ggaatcgact cggatatttt cgctgtgtcc
     2941 gcttctgacg cagataacaa tcttgcaaat aaagatgtag atgtactgtt attaggacct
     3001 caagttcgct ttatgaaagc tgattttgaa aaacgtctgg aaccaaaagg cattccttta
     3061 gacgtcatca atatggctga ttatggtatg atgaatggcg aaaaagtgtt agatcaagca
     3121 atcacgctca taggtgaata aatagaacag tataactatc gaacaagcga tccaaaaaat
     3181 agatcgcttg ttttttcgta tattttttta gggcgaataa tatgaatcat ttttggggta
     3241 gaaggcttct gacgagagat attaaagcaa tagactgtga ggaggtaaat ttaccaaatt
     3301 atgagaagat cgcagcagtg gaacgtttgg gcaactggat tctttgtcat cgctgtggaa
     3361 tgaggacgtc cgtcattgat ggacagcttg cagattacgg ttatttttgt acacattgtc
     3421 tatatttagg gcgatgcgac actcaacaag atttatattt gtttgatcag cctgaagcaa
     3481 aacctcgaga agtcgtcttt ttttggacag gacagcttac tgaaaaacaa acggaaatcg
     3541 caaagagaat actttgccat tcagaaaaaa gacatcatct tatttgggct gtaacaggtg
     3601 cagggaaaac agagatgctt tatcctattt tagtggaaac tttaaaagct ggaggaagag
     3661 tagcgatttg tacaccgcga gtcgatgttt gtaatgaatt gtttttacgc tatcgttatg
     3721 tttttccaga agaaaaaatc acgttgctcc atgggaatac agcaacggct tataccttta
     3781 cttcttttgt tatctgtacg attcatcagc tataccgttt ttatcggtcc tttgatttaa
     3841 tagtgatcga tgagatcgat gcgtttcctt ataacgggga tgcaggatta gctcatgctg
     3901 tgcaaacagc gattaaacca gacggacgat tcatctactt aagtgccaca cctgataaaa
     3961 agttactaaa ggaaatcaag cagacgtttg acatccacaa acttgctttg cgctttcatc
     4021 ggcgtttact tcctgaaccc gtcttggttt tcatgaataa ttggcgacaa aaatgcaaga
     4081 acaagaagaa gataaaatct ttggtgcgga tcattcatca attaattaaa gaaaaccata
     4141 tccttttatt ttgtccaagt atcacgatga tgcatcgttt gaaagccaca attcaagagg
     4201 ctcttccgag atatgagatc acagatgttt cttcaagaga caatcagcgg gcggaaaaag
     4261 tccagaaaat gcgagaagca aaatatgaca tactgttaac cacgatgatc cttgaacgtg
     4321 gagtcacatt tgaaagaata tccgttatcg ttttaggagc ggatcatccg gtttttacta
     4381 aatcttcttt ggtacaaatc gcaggaagag ccgatcgtaa agggccttat acgaacagtc
     4441 aagtatactt tttctatgaa gaaaaaactg cagctattcg aaaagcttgt caggaaatca
     4501 aagaaatgaa tgaagaaggg aagaagatgc tgtgaggtgc agttgctgca gtaaagatat
     4561 cgtccaaaat ttaacattca gcgagattat tggtgtttcg aaatacagat tacctcagac
     4621 ttgttcagtt tgtcgcgcac aatttcattt attggaaaca agaggctgtt ctggctgtca
     4681 aaaaccaatc catttcaatc tagagagtac gaaaaagaaa gtaaagtcta ttcccgttta
     4741 ctgcagtgac tgtcaagaat ggaagaagaa atatccgacc tatgattttc agcaccgtgc
     4801 cttttttgtg tacgatgaaa atatgaaggc ctggctcaat caatacaagt ttatgggaga
     4861 tatccgacta gcaggcacct ttgcatataa atggacgcag ataaaaaaag aatacccgac
     4921 atacatctat tgtccaatac ctttatctga aaaaaggcga acagaaagag gattcaatca
     4981 agtaagcgag atgttgaaag cagcaagaat tccttttcag atgctactca aaagagagaa
     5041 gcatctgctg gctcaagcaa aaaacacgcg aaaggaaaga ttggagatgc ctcaaccttt
     5101 tgaacttgca gtcacctttt ctaagataca aaataaaaaa atattgatta ttgatgatgt
     5161 ctatacaaca ggacagactc ttttccacgc agcagattgt ttgcaggtcg cacagccgaa
     5221 aatcatccga accatttctt tagcaagatg aattagatgt ggaaatttga tagcgattta
     5281 atttgcaaat aacctagagt tcgatataat agaattaaga agagggaaag agtggtcctt
     5341 ctccttcttg attgtggtag acgaaagggg taatttttat gtttagatat aatgtacgcg
     5401 gcgaaaatat cgaagtaact gaagcgattc gcgactacgt tgagaaaaaa gtaggcaaac
     5461 tagaacgcta tttcagtgac gcacccgatg caacggcgca tgtgaactta aaagtttata
     5521 cagaaaaaac agcaaaagtg gaagtaacga tcccattacc ttatcttgta ttacgtgccg
     5581 aagaaacttc tccagattta tacgcaagta tcgatttggt tgttgacaaa ttggaacgcc
     5641 aaatccgcaa attcaaaaca aaaattaatc gtaaatcccg tgaaacgggc atgaatactg
     5701 cagatgctac tgctttattc aatgaagagg acgaaaacga aaatgattct gaattagata
     5761 tcgtacgtac gaaacgcctt tcattgaaac caatggatag tgaagaagca gtcttacaaa
     5821 tgaacatgct gggacataac ttctttattt ttgaagatgc agaaacaaat ggtacaagta
     5881 tcgtttaccg ccgtaaagat ggaaaatacg gactgattga aacagactga tcagccttac
     5941 ttaaataaca acgaaaactt gcagcagcta ctgacagctg ccgcaagttt ttttgagttt
     6001 cctcctcttt tagatgaaat atgaataaat tatgaagttc ttactttaat acgcttaaga
     6061 tatttcaatt atctataact aatgataaaa tgaaaagtaa agtctacata gtctggataa
     6121 tacacagaag gaaaaggaga ttacacaacg catggccaat ttgattcgtt cacttatcga
     6181 aaacgataag aaagaactaa aacgtttaga tagcattgca aaaaaagttg aagcatacgc
     6241 tgaccaaatg gcagcattaa ctgatgaaca attacaggca aaaactgctg aattcaaggg
     6301 cagataccaa aaaggagaaa cattagatca attacttccg gaagcttttg cagtcgtccg
     6361 agaagcagca aaacgtgtat tggggttata tccacaccac gtacaattga tgggtggaat
     6421 cgtgcttcac gatggaaata tccctgaaat gcgtactggt gaaggtaaaa ccttgaccgc
     6481 aacgatgcca gtatatctaa atgctctgac tggcgaaggg gtacacgtag ttaccgtcaa
     6541 tgaatattta gcaacccgtg actcgacgga aatgggcgaa ctttataatt tcttaggatt
     6601 aacagtgggg ttgaatatca actcaaaatc ttcggatgaa aaacgcgagg cttataattg
     6661 tgatatcact tatagtacga ataatgaatt aggatttgac tacttgagag acaatatggt
     6721 cgtttaccgc catcaaatgg tacaacgtcc gctgaattat gccattgtcg atgaagttga
     6781 ctcaatcttg attgatgaag cgagaacgcc gctgatcatt tcagggcaag cagaaaaatc
     6841 cacagcttta tatacaagag cagacaactt cgtgaaacgt ttgaaagaag aagaagatta
     6901 taaaatcgat gtacaatcaa aaacaatcag tttaaccgaa gcagggatcg aaaaagcaga
     6961 agaaacattc ggtttggata acttatacga cattgaaaat actgcattga cccatcactt
     7021 ggatcaagca ttgagagcca actatatcat gcttttagat atcgattatg ttgtccaaga
     7081 cggaaaagta ctgattgttg accagttcac tggtcggatc atggatggac gccgttattc
     7141 agatggactt caccaagcaa tcgaagctaa agaaggcgta gagatcgaag acgaaacgaa
     7201 aaccatggcg acgatcactt tccagaacta tttccgtatg tacaagaaac ttgcgggaat
     7261 gactggtaca gctaaaacag aagaagaaga attcagagaa atttataaca tccaagtata
     7321 ccagattccg acaaatcgtc cggttatccg tgatgatcgt ccagatttgc tttacccaac
     7381 attacaaagc aagttccacg ctgtcgtgca agatatcaaa gatcgctacc gcaaaggtca
     7441 acctgtgtta gtcggaactg ttgcggttga aacatctgag ttattatcag atatgctgaa
     7501 tcaagaacgt attcctcatg aagtgctgaa tgcgaaaaat cactttaaag aagcagagat
     7561 catcatgaat gctggtcaaa aaggtgctgt gacgattgct accaacatgg ctggtcgggg
     7621 aacagatatc aagttaggac ttggggtaag agaacttggt ggtctagcag ttatcgggac
     7681 agaacgacat gaatctcgtc gtatcgataa ccagcttcga ggacgtgcag gacgtcaagg
     7741 agaccctggt atgtcacagt tctacttgtc attggaagat gatttgatga aacgattcgg
     7801 ttcagaacga atcaaatctt tcttagatcg gatgaagatc ggtgaggaag atgctgttat
     7861 ccaaagcaag atgctgacga aacaagtaga atctgcacaa aaacgtgtag aaggaaataa
     7921 ctatgacaca cgtaaaaacg tcttacaata cgatgatgtg atgcgtgaac aacgtgaagt
     7981 aatctataaa cagcgacaag aagtaatcat ggaagaaaaa agcttatcca agccactaat
     8041 gaacatggtc aaacggacaa tcagtcgtgt agtagatgcg catacacaat tagagaaaga
     8101 aaattggaat ttggaaagta tcatcgattt tgccggcagt actcttgtcc atgaagatag
     8161 tattactctt gcagatatcg aaggaaaaac agctcaggag atcaaagagt atctaaacga
     8221 acgagcacaa gaagtattta ataccaaagc ggctcaacta aatggtccag agcaactgtt
     8281 ggaatttgaa aaagtcgtga tcttgcgtgt tgtggactct aaatggacag accatattga
     8341 tgcaatggat caattacgtc agtcaatcgg cttacgtgct tatggacaga acaatccatt
     8401 agtcgaatat caaacagaag gctataagat gtttgaagat atggtaggcg ccatcgagta
     8461 tgaagtcact cgaatcttta tgaaagctga gatccgtcaa aacgtacagc gtgaacaggt
     8521 ttctcaagga acagcttctc actcagaaga cggagatgcc aaagaaaata gtaatactag
     8581 cgcgaaaaaa cagccaatcc atgtgaacaa ggtcggaaga aatgatcctt gtccttgtgg
     8641 aagcgggaaa aaatataaga actgtcatgg aaaaaatgca aactaatttc tgacagcagg
     8701 attggtgaga caaattcttt ttttaagaat tcgtctcacc ttctttctgt tatttgctga
     8761 ttctgttaga ctagatataa tgaaaaaagt atagtgaaag aaggaaaaaa catggaaata
     8821 tccgaaattc gaaatctcct gacagaaatg caggaaaagg tcacaagctt caggaggtct
     8881 ctttgactta gaccagctgg aagaagacat tgcggaagca gaagcaaaaa tggctgaacc
     8941 tggtttttgg gatgatagcg aagctgctca aaaagtgatc agtgaaaaca atgcaaataa
     9001 agaaacttat gatcaattcc attctcttgc tgaagaactg gaagaaatcg aattgatggt
     9061 ggagatgttg caggaagaac ctgatgcagc catgcaagaa gaagcagaaa cacgtatcaa
     9121 tgcgctagat gaaaaaatga agacatatga attatctatg ttgttagatg gtccttatga
     9181 tcgaaacaac gccatcgttg aattgcatcc aggggctggc ggaacggagt cccaagattg
     9241 gggcagtatg cttttacgta tgtatacacg ctgggcagag cagcatgggt atcaagtaga
     9301 gacactagat tatcaagcag gtgatgaagc aggaatcaag agtgtgaccc tactgatcaa
     9361 aggacacaac gcatttggtt atctgaaatc agaaaaaggt gttcatcgcc ttgtacgtat
     9421 ctcgcctttt gactcagcga aacgacgtca tacttctttt tgttcggtag atgtgatgcc
     9481 agagcttgat gaaacgatcg atatagaaat caatccagat gatttgaaaa tcgatacgta
     9541 ccgagcaagt ggtgcaggtg gtcagcacat caataaaaca gaatctgctg tacggatcac
     9601 tcacttgcct acaggaacag tagtagctag tcaggcacaa cgttcacaat tgaaaaaccg
     9661 tgagcaagcg atggggatgt taaaagctaa gctttatcaa ttagaagtgg aaaagaaaga
     9721 acaagaagca gctgcacttc gaggagaaca aaaagaaatc ggctggggtt cacagatccg
     9781 ttcttatgtt ttccatccat attctatggt aaaagaccat cggacaaacg tagagacagg
     9841 aaatgtccaa gcagttatgg acggtgacct tgatttattt attgacggct atttaaaaat
     9901 gcatctacaa taagaaccca ttgttaaatt gaaataaaat tgtaagattt tgcaagcatg
     9961 ttgaaacatt gccatgctat aatagctaaa gactgaaaaa gatatggaga gaacgccatg
    10021 attgaaatga aggatgtaat gaagaagtac tcaaatggta cgaccgctat ccgcaatctc
    10081 tcaattgtca tcgaacaagg agagtttgtc tatgtggttg gtccgtctgg agccggtaaa
    10141 tctacattca tcaaattgat gtatcgagaa gaaaaagctt caaaaggaac tttgaacgtc
    10201 gcaggtcatg atctaatagc aatcaaaaac cgcgaagttc cttatctacg cagagaaatc
    10261 ggcgtggtgt tccaagacta taaattatta ccaagaaaaa cagtatatga aaatgttgcg
    10321 tacgctatgc aagttatcgg cagaaaacct cgggatatca aaaaacgagt aatggaagtg
    10381 ctggaccttg ttggtttgaa acacaaagtt cgagtctttc ctagcgaatt atccggtggg
    10441 gaacagcaac gtgtctctat tgctcgggca atagtgaata cgcctaaagt acttatcgcg
    10501 gatgaaccga caggaaatct ggatccagaa aactcatggg aaatcatgaa attattagat
    10561 cggatcaatg cccaaggaac tactgtcgtg atggctacac ataacagtac gatcgtaaat
    10621 acgattcggc accgagtaat tgctatcgaa aacggccgca ttattcgaga tcaagcggaa
    10681 ggggaatatg gatacgatga ttagaacctt tttccgccat ttattagaaa gtatcaagag
    10741 tttgaaacgt aacggctgga tgacagttgc ttcagtcagt gcggtaacga tcacattgac
    10801 tttagtcggt gtattcatgg cagttatcat gaacgcaact aaattagctc aagatataga
    10861 aggaaacgtt gatgtctctg tattcgtaga tatcggtaca aaagacgaag agatgaaaaa
    10921 gctagaaaaa gaattgaatg acttagatca tgttaaaaaa gttgaatttt caagcaaaga
    10981 acaggaattg aaaaaaatcc aaaacgaaat gggtgattcc tggaaattgt ttgaaggtga
    11041 caataacccg ttatatgatg tgtatgtagt aagtgctact gatccttctt atacaaaaca
    11101 agtagcagaa gaagcagcag atttgaagaa tgtttctcgt tctgattacg gtggagcttc
    11161 ctcagatcgg attttccaaa tcgctggtgc agttcggaca tggggattag ttgcagcggc
    11221 attgctgctg ttcgtagcga tgtttctcat ttcgaacacg attcggatca cgattttgtc
    11281 tagacagcgt gaaattcaaa tcatgcgttt agtcggtgca aaaaatggat atattcgctg
    11341 gccgtttttc cttgaaggtg gctggatcgg tctgatcggt gcgattcttc cgattttgat
    11401 catcacattt ggttatgcat ggtttttcca attgatcaat ccatcattat tacgttccca
    11461 ttattcattg attcatccac aaaatatcgt ttggaaaatc aatcttttaa tggtcggcat
    11521 tggtgtgatc attggttcac ttggctcgat catttctatg agacgtttct tgaaattcta
    11581 ataccacaat ataaataaaa tgagaaagtt ggacagtttc gttccagctt tcttttttta
    11641 ttggctagga gaatagaagg aagcggcttt tttcccttta ctttaaaaaa caacttgggt
    11701 attcttaagt tggtagaaga agaggtgaag ccgtgaaaat tatcgttgac aattttttta
    11761 cgatcctttt gattatgaat attttgcttt cctttatcat cgtttttcga gaaagaaagg
    11821 aaactgctca aacatgggca tgggtgctcg ttttgatgtt cattccagtt gtaggattta
    11881 ttttatatat ttttttagga agagggattt ccaaagagaa aatctttgat ttgaagatcc
    11941 aagggaaaat cggtaagaac ctggaaattg aagaagacaa acaggcattg atgcgagggc
    12001 tttatcctca tccgcctact ggacaagtag atgtgaaaca attgatctat atgctcacgg
    12061 tatttgaaag tacgctctat acgactaaaa atgaaatcac tttatttaca gatggtcgtg
    12121 aaaaattcga tgcgctgatt gaagatatca agcaggcaga agaccatatc cacttccagt
    12181 attatattta tcgcagtgat gcattaggtg aagaagttcg agatgcattg acagaggcgg
    12241 caagacgagg ggtaaaagtc agagtccttc ttgatgcatg gggttccaca caagtttctt
    12301 ccagcttttt tcagaatttg aaaaaagcag gcggagaagt cgcatttttc tttcctctat
    12361 ttgttccgta tattaatcct cggatcaatt atcgcaatca tcgtaaaatc gtagttattg
    12421 atgggaaaat cggatataca ggtgggttca atgttggaaa tgaatatcta ggattggtaa
    12481 agaaatttgg ttattggcga gacaatcatt tgcgtattta cggagaagcg gtgtatacct
    12541 tgcaaaaccg ttttttgatg gactggaatt ctcagcatac aaaagaagta ggctattctc
    12601 ccaaattttt tccgtccatt cactctactg gtgatatagc tgttcaaatc gtgacaagcg
    12661 gaccagatac cgaacatgaa caaatcaaaa tgacttattt gaaaatgatt tcgttagcca
    12721 aacgggagat attgatccaa acgccttatt acataccgga tggatcgatt catgaagcac
    12781 tgaagttggc tcttctttca ggcgtaaaag ttcatatcca aatacctaac aaaccagatc
    12841 atcttctcgt ttactgggca acgtattctt tcgctgcaga gctaatcgag tatggagcaa
    12901 ggatcgaaac atatgagaat ggatttattc atgcaaagac gatgattatc gatggtggca
    12961 ttgtttcagt tggctcagca aatatcgatg ttcgttcatt tcgcttggac tttgaggtca
    13021 atacactggt ttatgatgaa cgaatggctt cacgtgtaag aaaagctttt tttgaagact
    13081 cgaaaatttc tacgcatctc acaaaagaaa tgtatgaaaa ccgaggaata atcatcaaga
    13141 tgaaagaagg attagcaaga ttgatttcgc cgctccttta aaacataaaa aggacatgag
    13201 aaccatcaac agatggagat ctcgtgtcct tttctaacgg ggccatttct gagagtacgt
    13261 cagaaatgga gaaagcgtct cgcatgagcg aaattaactt tggggtatct cttgtttttt
    13321 ggggaaagca agaaataagc taatatatgc tattaaaaga gacacatttc aaagccagta
    13381 tagcatagaa acttttaatg agatagtttt ttttacaatt atgagaattt attttgttat
    13441 ttaatgattt tgattcgttt tcatcaagta atgaaaatga taaaataaac atactttagg
    13501 attaaaggag atttctaatg aaaaaactta aattattctt accaataata ggattagcta
    13561 ttcttttatc aggctgttca aagtggattg atcgaggaga atccattacg gctgttggct
    13621 cttctgcttt acagccttta gtagaaacag ttgctgaaac ttaccagaca gaaaatcctg
    13681 gaaaattcat caatgtacaa gggggcggaa gcggaactgg tttgtctcaa gtacagtcag
    13741 gagcagtcga catcggaaac tctgatttgt ttgcggaaga aaaatcgggg atcgatgcct
    13801 cagcattagt cgatcatcga gtagcagttg ttgggatcac tcctattgtg aacaaggata
    13861 ttggcgtaaa agacatcagt atggaaaacc tgaaaaaact atttacaggc gagatcaaaa
    13921 actggagtga agtcggtgga aaagatcagc cagtagttat attgaaccgc gcttcaggaa
    13981 gcggaacccg tagcacgttt gaaaaatggg tattggacgg agaaacagct gttcaggcac
    14041 aagaacaaga ttcaagcgga atggtgcgcc agatcgttgc cgacacaccc ggtgcaatca
    14101 gctatgttgc tttttcttat gtttccgatg atgtagatac gttgaatgtt aacggtgtag
    14161 aaccgacaga tgaaaatgtg acaacgaaca aatggacgat ttgggcatat gagcatatgt
    14221 acacaagaga tcatccaaaa aaactgacaa aagaattttt agactatatc ttatctgatg
    14281 aagtacaaga taacatcgtt ggagaactag gttatatccc tgtttctcat atgaaagtcg
    14341 aacgtgattg gcaaggaaat atcgttaaag agtaatcttt acacaaaatt tacaaactta
    14401 gctaaacttt tacatgttat taaaaccaaa ttgatgttca atttgtacac tgagtgagta
    14461 tgttaaacct gattaagggg gagtcatctg tggaagatgt gaaaaaagta ttattaacaa
    14521 aatcaaaacg ttcaaaaatg gaacagcgtg gaaaattcat cagtttttta tgtatcgcgc
    14581 tgatcgtgtt ggttgtatta tctatttttt attttgtagc tagtaaagga ttggccacct
    14641 tttttactga taagatcaat gtatttgatt ttctgtttgg tacagattgg aatccaagcc
    14701 aaatcggagc aaatgggaaa ccaatggtag gggcattgcc tatgatcaca ggttcgttta
    14761 ttgtgacctt tttatctgct atcgttgcca cgccatttgc cattggtgca gcagtattca
    14821 tgacggagat ctctcctaaa aacggaacga agatcttaca gccggtcatc gagctgcttg
    14881 taggtattcc ttctgtcgtt tatggtttta tcggtttatc agttatcgtg ccttttgtca
    14941 gaagtatttt tggcgggact gggtttggta tcttagctgg tacatttgtg ttgttcgtta
    15001 tgatcttgcc gactgtcaca acgatgactg ttgatgcatt aaaggcagtg ccacggcact
    15061 atcgggaagc ttcattagcg cttggtgcca caagatggca aacaatctac aaagtcgtgc
    15121 tacgagcagc cgtgcccggg attttaacag cagtcgtttt tggtatggct cgtgcgtttg
    15181 gtgaagcctt agctatccaa atggtcatcg gtaatgcagc attgatgcca acaggtctta
    15241 ctactcctgc ttctacattg acgtccatct taacaatggg aatcggaaat acaatcatgg
    15301 gaacagttga aaataatgtg ttatggtcat tagcgctgat cttgctattg atgtcattat
    15361 tctttaatat cgtgatccgt atgattggga agaaaggaga gctgaaataa tgaacgcaaa
    15421 acgctcagat aaaattgcta caggtgtttt atatatagtt tcggggatca tcgtattgat
    15481 tttagcagct ttactacttt atatcttagt tcggggaata ccgcacattt catggtcctt
    15541 cttgaccgaa ccttccaaag cctaccaaga aggcggtgga atcgggatcc agctatttaa
    15601 ctcgatttat ttgcttttga tcacgatgat catcagtttt cctatttctt taggtgcggg
    15661 tatctatctt tcagaatatg ctggaaaaaa ctggctaaca gatgtgattc gcacgtcgat
    15721 tgaaatcttg agctcgttgc cttctgtcgt tgtcggttta ttcggtttct tggttttcgt
    15781 tattcaattc cagtatggtt tctcgattat ttctggtgca ttggctttga ctgtattcaa
    15841 cttgcctctt ttgactagaa cagttgagga gtctttacaa gcggtccatt acacacaacg
    15901 agaagcagga ttggccttag gtttgtcacg ctgggaaaca gtgataaaag tggtggttcc
    15961 agaagcgaca ccagggattt tgactggtgt gatcctaagt tctggaagaa tttttggtga
    16021 agcagctgca ttgatctata cggctggaca aagtgcacct gcgttagatt ttacgaactg
    16081 gaatccatta agtgtttcaa gcccgatcag cattttccgt caagctgaaa cattagctgt
    16141 ccatatttgg aagatcaata cagaaggcac gatgcctgat ggtgtttctg tttcggctgg
    16201 cgcttctgcc gtgttgatta ttgctgtttt actgttcaac ttcggcgctc gtgcgattgg
    16261 caaacgatta taccgaaaaa tgacttcagc ctaaggagaa gaagcaaaat gaaaaaatat
    16321 aactggaatg aaacaaatgt catcaccatg ccaaaagaga aagaagtagc actatatacg
    16381 gatgatctac atgtctggta tggcgacaat gaagcaatca aaggagtgga tctggaattt
    16441 gaaaaaaata agatcactgc actgattggt ccttccggct gcggaaaatc tacgtatctt
    16501 cgttcgttga atcgaatgaa tgatgggatc gctaatacga aaatcactgg gaaaatcatg
    16561 tatcaaggcg tagatattaa tgcgcctcaa gtggatgttt atgagatgcg caaacggatt
    16621 ggcatggttt ttcaacgtcc taatccgttc agcaagtcga tttatgacaa tattacattt
    16681 gctttgaaac agcacggcga aaaaaacaaa aagaaattag atgaaatcgt tgaaacaagc
    16741 ttgaaacaag ctgccttgtg ggaccaagta aaagacagct tggacaaaag tgccttggct
    16801 ttatctggcg ggcaacagca acgtttatgt attgctcgtg cgatcgcgat gaaacctgat
    16861 atcttgctgc tagacgagcc ggcaagtgct ttggacccta tttcgacagg aacggttgaa
    16921 gaaacacttg taaacttgaa agaagactat acgatcatca tcgtgacgca taacatgcaa
    16981 caagcagctc gtatcagcga ctatactgcc tttttctatt taggaaaagt aatggaatat
    17041 gacaaaacta acaagatatt taccagacca aaaattcagg caaccgaaga ttatgtgtcc
    17101 ggtcattttg gttaagacgg aggcctcaac atgacaaaag agattatttc atctaaagat
    17161 cttcatctgt attatggaaa gaaagaagca ctaaaaggga tcgatctaag cttcaatcaa
    17221 ggggaaatca ctgcgatgat cggtccttct ggttgcggaa aatccactta tctgcgttct
    17281 ttgaaccgga tgaatgacct gattccgaat gtaacgatca ccggaagtgt tttatataaa
    17341 gacaaagaca tctatggtcc aaaaacagat gttgttgaac taagaaaaga aatcggcatg
    17401 gtattccagc agccaaaccc atttcctttt tctatctatg aaaatgtcat ctatggattg
    17461 aaattaaaag gggaaaaaga taaaaaagtc cttgatcaag tagttgaaga aagcctgaag
    17521 gcagcgtctg tttgggatga tgtgaaagac aaattgcata aaagtgcttt atctttatcc
    17581 ggtgggcagc agcagcgtgt atgtatcgca cgtgttttgg cagttaatcc ggaaatcatc
    17641 ttattggatg aaccaacaag tgcgcttgat cctgtttcta ctgggaaaat cgaaagtatg
    17701 ctgttagaat taagagagcg gtatacgatg atcatcgtga cccataatat gtctcaagct
    17761 tcacgtattt cagataagac ggcattcttt ttagatggtc acttgatcga atacaatgat
    17821 acgaagaaga tattcatgaa tccagaaaaa caagagacag aagattatat ttcaggtcga
    17881 tttggataag gaggaaacgg aatgctacgt acacaatttg aagaagaatt gctgaatctc
    17941 cataatcagt tctacgaaat ggggatgatg gtgagcagcg ccgtgcacaa gtcagttcgt
    18001 gcatacatca aacacgataa aatcttagcc caagaagtta tcgaaaacga tataaatatc
    18061 aataacatgg aaacacgctt ggagaagaaa agttttgaaa tgatcgcatt gcaacagcca
    18121 gttacaactg atttgcgtat gatcattacg gtcatgaaag ctagctcgga tttagaacgt
    18181 atggccgatc atgcggtgtc tgttgctaaa tccacgattc gcgtaaaagg gcagacgaga
    18241 ataccggaaa tcgaaaaaga gatctccgat atgtccgatt atgtgaaaaa aatggtggac
    18301 aacgtcctag ttgcttatgt caaaacagac gaaaaagacg cacgtacgat cgccaatatg
    18361 gatcaaagag tcaatgacta tttcaatcga atctataatg caacaatcaa aaatatgcag
    18421 gaaaatcctg agacagtcat cagtggaaca gactacttga atgttgccag ttatctagaa
    18481 cggatcggag actatgtgac aaacatctgt gaatggatcg tctacttggc tacagggaaa
    18541 atcactgagt taaatacgaa ccacaatgag attttctaaa ctgaagctgt gattatgccg
    18601 tttgtttagg aaaagaggtt gtgaaatcag cggttgtgaa aaagctgttc tgcatcaagt
    18661 aataagaaca gtcaaacaaa aaatagctcc tcatattttt cgtttggctg gacttattac
    18721 cgcagatgca agcttttgaa caccgtttat tcgggaaagg gtttgtgaca aaacatgtca
    18781 caaacccttt tcatcatttc atagaaagga taaagaatgc aaaaaaaatt agcatcatca
    18841 ctatattttt tatttgtgtg cttacttcat gggttattca gcatcaagcc gctttatatg
    18901 aagtacctgt catcgagata gaaaaagtac gaataaaaga ggatcatcaa ttgattacag
    18961 gaaaactgat gaacacggat caaaaaggag aatcgattta tttatcggtt ccttatcaaa
    19021 aaaatcaaat agaaacagca aatataaaag cgagacaaat gtatctagtc caaatgcatg
    19081 gagatgaatg gcagctagta gcaaaaaaaa atgatggctg gcttttcttc ttcacaagta
    19141 tcttcattgg cgccatgttg cttattggtg gaaaacaggg tctatttgct ttaggaagtc
    19201 tcatcatcaa tatcgtttta cttttacttt tgttgctcat ctttactaaa acagaaggga
    19261 tttctctatt aaatatatct atcctgttcg tcgttatagg gatgattatt agcatattgg
    19321 ctttagatgg atggaataga agcagtataa tcaaaatttc agctgcagtt acgagtgttt
    19381 ttttagcatt ttttatttgt cttttgacaa tgaatcattg gaaagaccaa ggactgcgct
    19441 atgaggaact gaattatctg acacgaccgt atcgttctgt ttttctttct agcgtactga
    19501 ttggaacagc tggaggaaca ctcgataccg ttatcacagt cattgcgaca ttagaagaat
    19561 tgactaagcg acaaccagaa ataacagcga aacagttatg gctttcagga aggcaagtgg
    19621 gtcgagatgt gattggctcg atgaccaata tccttttgtt cgtctatatc agtgggtcag
    19681 ttccatcgct tcttttatat ttaggaaatc aatggtcgtt caaagaaacg atagaacagc
    19741 atttatcatt ggaattttta cgcgtcattg ctggcagctt aggaatagta ttatcgatcc
    19801 ctatctcggt cctctttttt ctgactgtaa ggaggctgaa acgatgaatg tacttattat
    19861 acttgttggg attttagcaa tcagcgtgct acttattggc ggtacgcaag gtatgtatat
    19921 tcttttaggc ttattcatca atttagggat ctttttcctg ttgttattcg gttatcatca
    19981 gaagtggccg atactggcat tgtcggtcat aggattttta ttgattgctg tggttatctt
    20041 attttttatc aacggttata atctaaaaat gcgtgcagct tttgtgagta tactgatctt
    20101 tttattttgt ttcctactat tgcttcctat cactgatttt ttagcaatcc aaggttttac
    20161 atcggtagaa ctagaagaat tatctggctt agataagact ttagcgattg attttagatt
    20221 acttacgaga tcgttgcttc tgattagtct tagtggtgct gttttagatg cgagtgtagc
    20281 aatttctagt gggacattcg aagtatatca ggcaaatccg caattatctt tttaccaatt
    20341 gcgccattct agtttcgcta ttgctaaaaa aatattggcg tcaacagtaa tgacacttct
    20401 ttttgcattc atgggaagtt ctttagcgtt gatcctctgg tttattgatt tagacattcc
    20461 ttttcagcaa gtgatcaatg aaaaatcatt tgttctagaa tatacgatgg ctattcttac
    20521 cacattgtcc gcactacttg ttcttccttt aacctgcgtg actacagcat atctctttac
    20581 aaaaaagaaa gaacaactca agatggagta aaagttacaa cttaagtcct atgacatttt
    20641 tactgaatca gtgcataata tggatgtaag gaaaagcttg caaacaattt tgcttaagga
    20701 ggcaatttcc atgaaagaaa gagaacgtat tttagattta gtgaaaaaag gaatcctttc
    20761 gactgaagaa ggcttagatt tattagaaag catggcaact gaaaaagacg aaaagcaaat
    20821 caaaaaagaa gcagacaagg taactgctag tcataaagaa aaagatcagg caagtcaatt
    20881 gatcgataaa cttgaaaacg gcgaagaaga aatttctgag ccagttgatc caaaggaaag
    20941 agaaagacaa gatcaagaaa acttagaaaa gatcttagat gaactagcaa ctgaagcgaa
    21001 taaaacttcg gcgcgtctag atgaagtaaa tgctcaattc gctgacaata aggcagcacg
    21061 caatgagaaa caggaagcat tgatgcagct gaatacaaaa gaagaattag gcgagttgac
    21121 agaagaagaa ttagcacagc gtcagacgtt agaagcagaa atcaaagaat tagaagctag
    21181 tggcgataca ctgttagaag aacaattgaa attagaagca gaattaaaag atatccgtaa
    21241 gaatcaatgg tctgagaaaa aagaaacgtt tactgataag tttgaattgc cagatgattg
    21301 gaaagatcaa gcaacagata cactgaatca agtaggcgaa aaaatgtcag aagctggatc
    21361 acagttaggc aaattcttga agaaaacatt ccaaactgtt tcagaaacag tgaatgacaa
    21421 catggaatgg aaagatgtca gcttacgtgt ccctgggatt gcgacaacga aatttgagca
    21481 tgaattttac tatgaagctc ctgcagcaag tatccttgat atcaaggcag caaacggaaa
    21541 cgtgacgtta aaaacgtggg acagcaacga tgtgaaagtt gaagctaaga tcaaacttta
    21601 cggaaaaatg ggtgctgaac catttgaagc attctctgaa cggagccaga ttgaggttaa
    21661 tgaagatcat atctccttcc aaattccaaa taaacgtgtg cgtgcagacc ttgttttcta
    21721 cttacctaaa cgagtatacg accacgcagc tattaaactt ctaaatggca acatcatgat
    21781 tgaaacattg gaagctaaag atatttatac aaaatctacg aatggtaaca tcatcgtgga
    21841 ccaattaact gcaacgatgc tggaagtaga aggtgtaaac ggcaatatcg atattcgaaa
    21901 cgggaacatc ttagattcta ttatcgaaac agtaaatggt acagtgacat ttggtgcgac
    21961 agcagaaaac ctttctgtgt ctttggtaaa tggtgatgta cgtcttacga ttaaagaaga
    22021 taatttgaaa aaagtagaag caagttcagt gaacggaaat gtcaaagtcg ctttaccaga
    22081 tggcatcggt ttggaaggac atgctaaaac aagtcttgga agtatcaaca gccgtctgtc
    22141 caattacgaa gtagttcgtg agaaaaaaga acggacaaac caaatgttgc aattccgtcg
    22201 tgtttcagat ggtgaaatcg cgcaagtgca actttcaacg acaacaggaa gtatctattt
    22261 aaaagataca gataaataaa gaggttgtga aagagatgtt cagccttgag caataagaag
    22321 cgattttgta aaatagcttc ccctatttta caaaattgtg ccttattgcc gatagtctat
    22381 ctcttgaaca ccgtttacca agagattatg aacctgccaa ttatcgccga gtcacaagga
    22441 ataatttcag aaaaagctcg tcacgtttta tgaatatggt aatttgtgac cgagaatttt
    22501 gcagaaagac accgattaag aagaaggttg tcacaacgat tttcttcatt taaaatagta
    22561 gaacagaggt gagacatgat gaaaaaaaag ttgacaaaat ctaataaaaa tgttgttttg
    22621 acagggactt tagcaggaat aggagaatac atcgggattg atccgactgt tatccgtgtg
    22681 ctttatgtat tcctgagctt agttgctttt ggaagtccaa ttattttata cattttatta
    22741 gctttgatta tcccatctgc gcgttctgca cgaacaaact atggacataa caatgcatat
    22801 tatgaaaaaa acaattatcg tgaacaaact cgtgaacaaa caaaaaaacg caaagaagcg
    22861 gaaaaaatcg atgatgacga atggagtgac ttttaatgtc gtactttcaa cgtattttag
    22921 ttaatacgtt aacattcata tcattggctg ttctttttcc tgcgatggtc catgtgagca
    22981 gtatttggac ggccgttctt gccagcttcg tattgtctgt gttgaatatg tttgtcaaac
    23041 cgattttgac gatactgtcc ctgcccttta cattagtcac attgggattg tttacgtttg
    23101 ttatcaatgc agcgattctt aaaatgacct ccttttttgt gggagcagaa cattttagtt
    23161 tttcttcatt cggtgcagcg ttgttagtag ctatcttgat gtcgcttgtc aatgtaattg
    23221 tcagcgaaca taacagtaga aaataaaaga aagctgtgcc gaactatcgg cgcagttttt
    23281 ttatttagaa tcaggaatgc gttttctatt acattggtaa ttaaataaaa acgttttttt
    23341 taattggtag tacttttggc tagacaatga taaaatgata gagaacactc taatagaaat
    23401 gaggtagcat gaatgtcaga agttgtaaaa gttagccaat tagtgaaaaa attatctttg
    23461 gaaatcgtga caggcgacga gaaaagctta gaccgtgtca tcgtaactgg ggacatctca
    23521 cgcccaggac tagagttgac tggttatttt aattattatt cccataatcg tttacagcta
    23581 tttggcagta aagaaatcag ttttgcggaa cggatgatcc cagaagagcg tttgatcatc
    23641 atgagaagat tatgtagtga agatatgccc gcatttatta tttcaagagg attaccagct
    23701 ccagaagaaa tgatccaggc agcaaatgaa aatgggatag ctgtactgcg ttctcccatc
    23761 tcaacttctc gtttattagg agaattatcc agttatttag atggtcgcct tgcccctcga
    23821 acaagtgtcc acggtgtttt agtggatgtt tacggattag gtgtactgat tcaaggtgac
    23881 agtgggatag gaaaaagtga aacagcgttg gagttgatca aacgcggcca tcgattgatc
    23941 gcagacgatc gagtagatgt ttatcagcaa gatgaattaa cggtgatcgg agaaccgcct
    24001 aaaatcctgg aacatctgat tgaaatccga ggaatcggga tcattgatgt aatgaatctt
    24061 tttggtgcga gtgcggtacg tggctctatg caagtccagt tggctgttta tctggaagca
    24121 tgggcaaaag ataagaaata cgaccgtctg ggctctgaag acacgaccgt tgaaatcgcg
    24181 gaagtgggca ttccacaaat aaaaatccct gtcaaaacag gacgaaacgt tgcaatcatc
    24241 attgaagtag ccgcgatgaa tttccgtgca cgtacaatgg gctttgacgc aacgaaaaca
    24301 tttgaagaaa gattaagtcg cttgattgaa gaaaactcag gcaacgacta gcaaggagtg
    24361 acaatatgtt agcacaaatc aatcgagttg cttttgagct tgtagggatt cctatctact
    24421 ggtatgcatt gatcatcgta tcaggtatca ttatcgctat gtggcttagc agcagagagg
    24481 ctgttcgggt aggattaaaa gcagatgatg tgacagattt tatgttgtgg ggattgccta
    24541 tctccattat tggcgctcgc ttatattatg ttttatttga tcttccccag tatatagctg
    24601 atccaatcca aatattcaat atccgttcag gaggcttagc tatttatggc ggactgattg
    24661 caggcgcgat cacgctgtat ttttttacta aatatcattt tatttctttg tggacttttt
    24721 tagatatcgc tgcaccaagt gtattattag cacaggcaat cggtcgttgg ggaaatttca
    24781 tgaaccacga agcatatgga ccagacacga cgcgaagctt cttagaaagt ctgcatttac
    24841 cgcaattcat cattgataat atgtatatcg aagggatcta tcgtcaacca acatttcttt
    24901 atgaatcact ttggagtttg attggattta ttttgctttt gtttttacga aagaaaccac
    24961 accttttgaa aaaaggcgaa gtccttttat gttatctaat gtggtatagt ttcgggcgtt
    25021 tctttattga aggattacgt acagacagtc tttacttttt aggtgtgatc cgtatttctc
    25081 aattattatc tgctgtgtta tttgtcggta cattgattct atttatttgg cgcagaaaaa
    25141 aaggaaattt accggattat gaccgctcat taggaaaaaa tcaacaaatt atctgatttt
    25201 tagagcgtta aaaatgaaaa aaatttcagg agtggtaaaa tgaattatag agtagcggtt
    25261 ttaggaccag gctcttgggg tactgctttg gcacaagtcc tcgccgaaaa cggacatgac
    25321 gttcggattt ggggacatca tgcttcacaa gtagaagaaa tcaacaccca acacacgaat
    25381 caacgttatt taccagatca ttatctaccc gattccatcc aagcattcac caatatggaa
    25441 gatgcagtaa aagacgcaga tgcggtgcta ttcgtcattc caacgaaagc aatacgttct
    25501 gttgcaaaag aactagtgcc taaattgaat acaaagccag tgatcatcca tgcaagtaaa
    25561 ggattagaac agggaacaca taaaaggatc tctgacatct tgttagagga aatcccagca
    25621 gaaaaaagaa aagctgttgt tgttctttct ggaccaagcc atgcagaaga agtagcaaaa
    25681 catgacatca ctacgatcac agctgcaagt acagatgcaa aagcagcaaa atatgtacaa
    25741 gagctgttca tgaataatta ttttcggatc tataccaatc ctgatgtgat cggtgtagag
    25801 atgggggcag ctttgaaaaa tattattgct attggtgctg gcgctattca cggcttaggg
    25861 tttggcgata atgcaaaggc agcaatcatg acgcgcggat tagctgagat cagccgttta
    25921 ggtgtagcaa tgggtgccaa cccgctgacc tttatcggat taagcggaat aggcgatcta
    25981 gtcgttactt gcacaagtgt tcattctagg aactggcgcg caggcaactt gttaggacaa
    26041 gggcagcccc tagaagaggt gctagccaat atgggaatgg tcgttgaagg tgtgaatact
    26101 acccaagcag cgatggaact agcccaaatg atggaaatcg aaatgcccat cactcaaaca
    26161 atctatgaag tgctgtatga acaaaaagaa atcaaaacag ccgcaaaaga aatcatgtta
    26221 cgtgatggaa aaaaagaaaa tgaattctca ttaacaattt tttgaaggag cgaatgtata
    26281 tgaaagttaa aaaagcagtt attccagcag caggattagg aacaagattt ttaccagcaa
    26341 caaaagctat ggcaaaggaa atgttgccaa ttgttgataa accaacaatc cagttcatcg
    26401 ttgaagaagc gttgaaatca ggtatcgaag acatcttgat cgttacagga aaagctaaac
    26461 gaccaatcga agatcacttt gattcaaata ttgaattaga aacaaaccta agtgaaaaag
    26521 gaaagaccga attattgaaa ttagtagaag aaacgacaga tgtcaacttg catttcatcc
    26581 gtcaatctca tccaaagggt ttaggacatg ccgttcttca agcaagagcc tttattggaa
    26641 atgaaccatt tgtcgttatg ctaggcgatg atataatgga agacgaagta ccattgacac
    26701 agcaattgat cgatgactat gagcgtacac acgcttctac gattgccgtt atgcaagttc
    26761 ctcacgatga aacttcaaaa tacggaatca tcaacccagg ggaagtactg gaagatgggt
    26821 tatacaatgt caaaaacttt gttgaaaaac cagatccaag caaagcacca agtgatttag
    26881 ctattattgg gcgttactta ttgactccag aaatctttga tgtattatca gaacaacaac
    26941 caggtgctgg aaatgagatc caactaactg acgcaatcga tacgttgaat aaaacacaac
    27001 gcgtatttgc ccgtaaattc acaggtaaac gttatgacgt tggtgacaaa tttggtttta
    27061 tgaaaacaag tattgaatat ggtctgacac atccagaagt aggggcacct ttgcgtgaat
    27121 acatcatgtc tttaggtaaa acattagaag cagaaggaag caagatcaat cataatccaa
    27181 aaggaataac agaaatcaaa gaagaaaaga aaaaataatc tttttttcta acgagcttca
    27241 gctgctaatt gaataaaaag tagttgaagc tcgtttttat ttgtttaaaa aacacgaaaa
    27301 tgctatcctt atagaaaaga gagacaagaa gggagtttga tcatgtcagg aggagaaatt
    27361 gcaggattga tcgcagcaat cgcctttgcg atacttgtcg ttttcatcgt tcgcgtattg
    27421 ttgcaagttt ccaaaacttt agaaaaagta gaccagactg tcgcagaagc aaatacgaca
    27481 attgagatcg tcacacaaga cgtagatctt ttatctagac aagtagaagg cttgctagtc
    27541 aaaagtaatg agctgctctt ggatatcaat ggaaaagtcg caacgatcga tccattgttc
    27601 acagcagttg ctgatttaag tgaaagtgta tcggaactta atcattctag taaaaacatc
    27661 gtgacgaaag tcggcggaat cggaaaaacg acagctaaag cgactgttgc cggaaaagtc
    27721 ggtggaacag cgatgaaatt ttttaaaaac aaaaaacata ctgaaacgac aggaggaaaa
    27781 taagaatggc taaaaaaggt ggatttttat taggtgcagt agttggaggt acagccgcag
    27841 caatcgccgc attattgtta gcacccaaat ctggtaaaga acttcgggat gaattggctg
    27901 ctcaagcaga tgatttgatg gacatggctt cagattacac agatatggca aaagaaaaat
    27961 catctgagtt aacaggtatt gcaaaagaaa aagcgcaagg tctttctcaa caagcagggg
    28021 atctagcagg caatgttaag cagaaggtaa gtgaatcagc agaggaagca ggaagtgaat
    28081 cagatgatat cttgttaaac ctcaaagaac aatcagctga tttatctaat cgtttcaaaa
    28141 aaacagcaga agatctgaaa aagccagcag acgaattagg tagtattgct aaagatactt
    28201 cagaagatat catgatcgat gtaaaagatg ctgcagataa agcaggagag acggtcaatg
    28261 aaggaatgtc agaagcaaaa ccagttgcag atgacacaaa agaaatgttg aaaatggcaa
    28321 aagaagatgc aaaagaagct aaaaaagaaa tgaaagatac aatgacagat tcgactacca
    28381 atcactaaga ttactcattt ctagaataaa aaaggtgaga gcggaccagc tctcaccttt
    28441 tttattttca ttactctaaa acaagtaatt cttttggtgt agtcgtgaat ggcacacaac
    28501 catcacttgt cacatacaca caatcttcga tacgtacccc tactttttca ggaatataga
    28561 ttcctggttc aagagagaag cacatccctt cttcaatcac taagtcgttt ccttctacaa
    28621 gagaagggaa ttcgtggacg gttgttccga ttccgtgacc taaacggtga ttgaaatatt
    28681 cgccgtagcc atagctgttg atcacgtttc gtgcgatttg atcaagttca cctgctgtca
    28741 cacctgggcg aactgcctct tgtgcggcaa gctgtgcttc aagagtgatg ttatagattt
    28801 tttcttggaa ctcagttggt ttttgataag caactgtacg agtagcatca ctgcaatagc
    28861 cattccaaat aacgccaaga tcgaataaca ctagttcatt tggtgaaacc ttcgtgcttc
    28921 ctggtgtacc atgagggctt gcagcatttg ctcctgcaag gactaatgtg tcgaaagaca
    28981 tttgtgaaac accttgtttt tttaattggt attcgatttc tgcgataatt gcttgttcag
    29041 ttacgccttc ttttactgtt ttgaagccaa tttcaaaggc aacatcggcc catttaccag
    29101 cttcgatcag tttgtctttt tcttctaccg ttttgatcag ttgcagacgc tggataatag
    29161 aagttagatc ggtgtaagaa tctgtacctg tcaagatatc agagatagca tcgaagcgat
    29221 cgacagtcag agcattcttt tcaataccta cattaccagg ttgcccatag cgtgcgcgaa
    29281 gctcttttcc gatgatctcc caaggatttt cgctgtctaa gtagccacgt acatcgtacg
    29341 cccagctgct gttttgagca tcttcaactt ccagagcagg agtgaaaagg aaaggatctt
    29401 gttccagagg gataaaaaga gctagtacac gctcgtgagg atcactttca tatccggaaa
    29461 agtaagcgat gtggccaggg tcgctgatat aagccaattc tacttggtgt tgtgccatcc
    29521 attgacgtag ttcattgatt tttacgttat tcatttggca acctactttc taaattaatt
    29581 ttgtattcaa taattgtacc actgattgat taaaagacca acgttcttaa aactgaaaaa
    29641 agtaaaaatt ttcatgaaaa cagagataaa acggttgcaa ttgttgagat tatttgatat
    29701 cctattttag aaatgtaaga cgtattttca gaaaacatat aaaatatgaa taggagaaca
    29761 caaatggaaa agcaaacaat cactatctat gatgtagcga gagaggcaaa tgtttcgatg
    29821 gctactgttt cacgtgtcgt aaatggcaat ccaaacgtca aaccagcaac aagaaaaaaa
    29881 gtcttggaag ttatcgatcg cttggattat cgtccaaatg cagtcgcacg tggcttggca
    29941 agtaagaaaa caacaacagt tggggtaatc attcctgatg tcagcaatat gttctttgcc
    30001 tctttagcac gtggtatcga cgatgtggca acaatgtaca aatacaatat catcttagca
    30061 aactcagatg gcaacgatca aaaagaagtc aatgtattga ataatttatt ggctaagcaa
    30121 gtggacggcg tgatctttat gggtcaccat atcacagacg aaatccgtgg agaattttca
    30181 cgttcaaaaa cgcctgttgt actagctggt tccattgatc cagatgaaca agtaggtagc
    30241 gtaaacattg attatgtgtc agcaacaaaa gatgcagtaa ataaattagc aaaaagcggc
    30301 aataaaaaaa tcgcttttgt cagcggtgcg ctgattgatc ctatcaatgg tcaaaaccgt
    30361 ctaaaaggct ataaagaagc attaagcgaa aacaatctac catatagcga aggattgatt
    30421 tttgaggcac aatacaactt taaagatggt ctgtctctag ttgaccggat ccataatagc
    30481 ggtgcaacag ctgtctatgt ttcagacgat gaattagcca ttggtatctt ggatggttta
    30541 ttagaccgcg gcgtaaaagt accagaagaa ttcgaaatca tcacaagcaa caattcttta
    30601 ctgacagaag tagctcgtcc tcgtttgaca agtatcacac aaccattata cgatttagga
    30661 gcggtatcca tgcgcctatt gacgaaaatg atgaataaag aagaagtcga agaaaaaacg
    30721 attatcttgc cttatggaat cgaagaaaaa ggatcaacta aataataaaa gatcaatcat
    30781 ttaaaggttg ttccaaaagt ggtcaacttc tggaacaacg tttatacgag aaagggggtt
    30841 atgacaaaat tttgtcataa cccctttttt tttgtgactt taaaaaaaga gaaaatggcg
    30901 gaaacaaacc ttacgtccat cgtatacgac acaatctgtg cgtagatgct acattttgtt
    30961 cactactatc aaaattactt taaaatgaaa acgtgataaa aaagaaagta gaaaagtaga
    31021 taagtaactc taaaaaatcc aaaaaaatcc aggctcgatt gcgtacttat cttaaaggag
    31081 aaacaatcca tcatatataa cccgaaagga gacttctatt tgcctaagaa aagacgaaag
    31141 accatagtca tcattttgac cattcttact ataggaatcg gagggaaatt ttacatggac
    31201 aaaagagaaa tgcaaagaca gaaagattta ctcgcagtag aaaaacagtc cgtcaaagtt
    31261 ctgaaaaata cctttgcgga tatcaaggaa gtgaagattg aaaaatctgc tcgaaatgaa
    31321 atgacgggtt cataccggat agttattcta atgactaata aacaagatca atcgatttat
    31381 ttttcttata gtttttggaa agaaagaaat gagataggaa gttacggaat agttgatgaa
    31441 aaaaagcaaa aagaaggaaa tacgctcaat aaagttaaag taacttactc taatggaaat
    31501 gaggaaagta tttaatgatt actgacaaac aatatagtga attaagtgat gctgtttact
    31561 ggctagatcc taaacataga gactatgtac ctgaaatgca agaaaattta agttttaaaa
    31621 ttaacggcac tatgtataaa atcctaaaaa tcaaaaactc cttcgacggc atgcaggcga
    31681 tggctgtagc gccaatcgtc catagcaaat tggagaaaaa tttcaaaaat aaaaaaattc
    31741 ctgcaaactt tcgcgtactt aaataatgaa ggtgtcaata cttacataaa aagaaaggag
    31801 gcacgttctt gtcaaggaaa ataagaaaac tcagcttagc cattttatta atcgtcatat
    31861 tagggatcgg agggaaagtc tatatggaca aaagagaagc acaaaggcag aaagatttac
    31921 tcgcagtaga aaaacagtca gtcaaagttc tgaaaaatac ctttgcggat atcagggaag
    31981 tgaaggtaga agaatttaaa aaaaaccctg ttaccggaag ttatggtgca cttgtgacaa
    32041 tgacaaataa tgaagggaaa tctgtttatt ttgattatag tttttggaag gagagaaatg
    32101 aacttggtgg ttttggcatt gagaacagag cggtacaaaa gacaggagtc actataaaaa
    32161 gagtaaaagt aattttttct aacggacagg aggagttagt atgacaataa acgataagca
    32221 ttacaatgat attagtgaga aggtttattg gctagatccc aaatatcccc gctacaatga
    32281 gggatacaaa aagaactctg ttaaagaatt tgctggtatg gaatttcaaa tcctacaaat
    32341 caaagactca ttggacggca tgcaggcgat gtctgtagcg ccaatcaaga acggtaaagt
    32401 ggatctttct caagtagtga ttgcttttgc aggaacgaac ttaagtgatt ggaaagattt
    32461 ggaaacggat gcaagaagta tcatgaatct ctacgggaat agaccaggag caagtttttc
    32521 tgaaaaagca acaacaaaca agattttatc cgttagtgga ttgaaaacag cggggcaaat
    32581 caattcagcg attgcatttg ctaatgaagt aaaaaaggaa tatggagaag ctgctatttc
    32641 tactaccggc cattcattag gtggattctt agcattatat gtagcagcag agaaacaatg
    32701 gaaaaatgtc ggcttcaacg ggccggaccc gtatgatctc ttatcaccag aagcaaaaaa
    32761 atgggtggaa gaaaatcccg gtatgctcac caattaccga aatcacgccg attttatcgg
    32821 taattttggt ggtaatggca caggtgctga agttcggatc agtatggaaa cagcattctt
    32881 gaatctgctt gcttcccaca gtttaacctc ttggaagttt gatgaaaatg gaaagctgat
    32941 tattcctgaa aatgaaaaca atgttaaagc tcgaaaacaa cagaaagaaa accaagcgac
    33001 ccagttgttt tcctatcagt tgcaagaact gaacaacata cgtaaaaaac tacgattaag
    33061 tggcggccag ctgacagtta atgaacaaat ttacttagat gacacacaag ctaggttagt
    33121 agtggaaaag ttatcggcaa aaatgaaaag cgctatggaa gaagttatcc gcatcaatca
    33181 aagaggaatt tttgagctag agaaaaaatg gcaagaaggc ttgcggcaag cacgtgttgt
    33241 agccccttcg ttgactgaac aggaaatcat agaagcatta gctagtgtgg gcgccacaaa
    33301 agaaaactta gtcgattctg cgaaagaagt ctatctgcta cgaatcaata aggcaagacg
    33361 tatgggggag gcctttgata cattggcgaa ggagatccaa gacaaaatca atgaaatcgt
    33421 ggcacgagat cgagaattag cacagcaatt caattaaagg agaagtagat gaaactagaa
    33481 gaattggaaa gagattatcg aagtgaacga agacaattag aagctcaaga agatgaattg
    33541 atccacttta agaatcaagg attacgattg atggagcaag aggagcagcg actgcatcat
    33601 gtattaaaga acttagcttt agataatgag cctctgtttc aagcaagacg aagttattcg
    33661 aattatcagg agcattatca acaagcccta caagtagaac gacgaaaact ctatcagcag
    33721 caagatgaac tagaacgttc ctatcgtcaa aaaatcagac aaataactat ggagaaatca
    33781 tcttatgaat gaatttttaa tgcaattact aaaagaatta gagaaaaatc tacatcgatc
    33841 aaatgtacgt tcagttggta tggggatatc tcagcaaatt tgggcagaaa aacgattgac
    33901 agatcaaaac taccaaatga ctgcagaaga agctgctcaa gaattttcta accgacatgt
    33961 acacttgaaa ggatcagtaa aagggcaatt tgcagaaaaa ctaactgaat tattccaaag
    34021 acaagaaaag aaattaaaac agtttcgtta gtaaagttat atagaaaaat tttgtgaaaa
    34081 tggtt
//
LOCUS       DAHHZJ010000014        30881 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.014, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000014 DAHHZJ010000000
VERSION     DAHHZJ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.014"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(175..1461)
                     /locus_tag="NBL02_000538"
     CDS             complement(175..1461)
                     /locus_tag="NBL02_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCD2571249.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(1454..2722)
                     /locus_tag="NBL02_000539"
     CDS             complement(1454..2722)
                     /locus_tag="NBL02_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCD2571250.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            2974..3435
                     /gene="mscL"
                     /locus_tag="NBL02_000540"
     CDS             2974..3435
                     /gene="mscL"
                     /locus_tag="NBL02_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCD2571251.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(3494..3940)
                     /locus_tag="NBL02_000541"
     CDS             complement(3494..3940)
                     /locus_tag="NBL02_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD2571252.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(4033..4278)
                     /locus_tag="NBL02_000542"
     CDS             complement(4033..4278)
                     /locus_tag="NBL02_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HCD2571253.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(4393..4971)
                     /locus_tag="NBL02_000543"
     CDS             complement(4393..4971)
                     /locus_tag="NBL02_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCD2571254.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(5048..5578)
                     /locus_tag="NBL02_000544"
     CDS             complement(5048..5578)
                     /locus_tag="NBL02_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HCD2571255.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(5582..6442)
                     /gene="rlmB"
                     /locus_tag="NBL02_000545"
     CDS             complement(5582..6442)
                     /gene="rlmB"
                     /locus_tag="NBL02_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCD2571256.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(6435..6830)
                     /locus_tag="NBL02_000546"
     CDS             complement(6435..6830)
                     /locus_tag="NBL02_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HCD2571257.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(6827..8239)
                     /locus_tag="NBL02_000547"
     CDS             complement(6827..8239)
                     /locus_tag="NBL02_000547"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCD2571258.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(8236..8772)
                     /gene="cysE"
                     /locus_tag="NBL02_000548"
     CDS             complement(8236..8772)
                     /gene="cysE"
                     /locus_tag="NBL02_000548"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCD2571259.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(8858..9097)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(9094..10554)
                     /locus_tag="NBL02_000549"
     CDS             complement(9094..10554)
                     /locus_tag="NBL02_000549"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCD2571260.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPKMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(10724..11881)
                     /locus_tag="NBL02_000550"
     CDS             complement(10724..11881)
                     /locus_tag="NBL02_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HCD2571261.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(11915..13285)
                     /gene="radA"
                     /locus_tag="NBL02_000551"
     CDS             complement(11915..13285)
                     /gene="radA"
                     /locus_tag="NBL02_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCD2571262.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(13348..13875)
                     /locus_tag="NBL02_000552"
     CDS             complement(13348..13875)
                     /locus_tag="NBL02_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCD2571263.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            14105..14911
                     /gene="proB"
                     /locus_tag="NBL02_000553"
     CDS             14105..14911
                     /gene="proB"
                     /locus_tag="NBL02_000553"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCD2571264.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            14908..16155
                     /locus_tag="NBL02_000554"
     CDS             14908..16155
                     /locus_tag="NBL02_000554"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCD2571265.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            16231..16575
                     /locus_tag="NBL02_000555"
     CDS             16231..16575
                     /locus_tag="NBL02_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571266.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(16692..17018)
                     /locus_tag="NBL02_000556"
     CDS             complement(16692..17018)
                     /locus_tag="NBL02_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571267.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(17633..18835)
                     /locus_tag="NBL02_000557"
     CDS             complement(17633..18835)
                     /locus_tag="NBL02_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD2571268.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            18952..19467
                     /locus_tag="NBL02_000558"
     CDS             18952..19467
                     /locus_tag="NBL02_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCD2571269.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(19666..20112)
                     /locus_tag="NBL02_000559"
     CDS             complement(19666..20112)
                     /locus_tag="NBL02_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HCD2571270.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(20159..21637)
                     /gene="msr(C)"
                     /locus_tag="NBL02_000560"
     CDS             complement(20159..21637)
                     /gene="msr(C)"
                     /locus_tag="NBL02_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HCD2571271.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(21991..22620)
                     /locus_tag="NBL02_000561"
     CDS             complement(21991..22620)
                     /locus_tag="NBL02_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCD2571272.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(22604..23068)
                     /locus_tag="NBL02_000562"
     CDS             complement(22604..23068)
                     /locus_tag="NBL02_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCD2571273.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(23555..24823)
                     /locus_tag="NBL02_000563"
     CDS             complement(23555..24823)
                     /locus_tag="NBL02_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HCD2571274.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(25054..25791)
                     /locus_tag="NBL02_000564"
     CDS             complement(25054..25791)
                     /locus_tag="NBL02_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCD2571275.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(25858..26426)
                     /locus_tag="NBL02_000565"
                     /pseudo
     CDS             complement(25858..26426)
                     /locus_tag="NBL02_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
     gene            26554..27426
                     /locus_tag="NBL02_000566"
     CDS             26554..27426
                     /locus_tag="NBL02_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCD2571276.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            27768..28640
                     /locus_tag="NBL02_000567"
     CDS             27768..28640
                     /locus_tag="NBL02_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCD2571277.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            28637..30595
                     /locus_tag="NBL02_000568"
     CDS             28637..30595
                     /locus_tag="NBL02_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCD2571278.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
BASE COUNT         8796 a         6485 c         5648 g         9952 t
ORIGIN      
        1 attacttcgt ttgattttat tttaatataa gttgattaaa agagagcatg atttgtttta
       61 ttttctcact aatttttcca acacaactaa gaataccata aaagctgatt ttggaggagg
      121 ttcctactac aagtctaaaa cgaaatttaa gacttttcct cataaaatac tggtttattt
      181 tggtctcatg taaaactcgc tgaaggcctc ttcacttata aatgcttccc cagctgccag
      241 tacgtctttc atttggatcg aatcgatgat ttcaggaaga tcaaataaag ttcgatcgcc
      301 aaataaactt tgcgtaaatt gattggcaat atattcgatc gaatttaatg attggaaata
      361 ttgtccaagc attttcttct tcagcaaatc gagatttgtt tctgacactt ccggatcatc
      421 agcgaatcca agaatgattt ctttgacttt ttctgctgct ttctctggtt cgtccgtatc
      481 tccgctgaag tccgcaaagt gaaattcacg atccaagctg aattcaaaac caaagctgtc
      541 atcgataatt ccttgattgt acatcgctag atagtttcga gaagtattgc ctaaaatcat
      601 ttggaacaac agattgattg ctgttttata gcgtaacagt tctgttcctt cttgcggcaa
      661 tgtgtctaat cctttaatac caagtacaaa tttatctcgt gtaatggctg cttccaatgc
      721 actttgtttg atgatttctt ttgtgttttc agggaaataa cgtacaattt cttgctttgg
      781 agggaaattt tttgcttctt ggttttcccg aataagtttc atcaatttct caggctccat
      841 cttaccaact acaaataaaa ccatattgct tggctgataa aacgtccgat agcacgtata
      901 caagtcttga gctgtgattt tgtcgatact ttcaaccgtg cccgcaatat cgatatgcaa
      961 tgggtgtgta ggatacaagt tatttagtat gccaaaaaac atccgccagt tagggtcgtc
     1021 ttcatacatt tggatttcct gaccgatgat tcctttttcc ttgtttacag tttcttctgt
     1081 gaagtaaggt gcttgtacaa aatcgatcag agtcgtcaga ttcttttcta cctgatccgt
     1141 cgtagaaaac agatagctgg ttttcgtaaa acttgtgaaa gcattggcgg aagctccttg
     1201 tttcccaaat aattgaaaga catcaccatc ttctttttca aacaacttat gttctaaaaa
     1261 atgggcaatt ccatctggaa ctttcacctt ttctttttca ccatagggaa taaattcgtt
     1321 atcgatcgaa ccataattcg tactgaacaa accatatgtc ttgtgatagt catttttagg
     1381 caacaaatac acggtcaaac cgtttggcag tacttcatga taaagtgttt cattgatttg
     1441 ctcatattct gttttattca tttgcatttc ctcctgctaa aaagaagatt gcctgcaatt
     1501 cgatttgctc agctacttgt tgtatctctt tgatcgttac gctctccata cgtttcaacc
     1561 attcttcatc agataatttt gtttccggca gccagctgtc caaaaaagaa gcttcgattg
     1621 ccgcttgcgg agagtctaga gaaagcagat attggtttct cagcattgct tttgtctgag
     1681 acagttccaa ctctgttatt tcaccatttc gcaaactttc taattgctcg tgaatcagat
     1741 gaagtacttg atttcggttc ttttcatcga taccggtttg gacactcatg aatcctcgga
     1801 acgtatccac gctactagag gcataataag ctagactttc tttctctctg acattcatga
     1861 aaagtttaga gtgaggaaaa ccgccaaata aaccgttaaa gaccattagc gcaaaacgtt
     1921 ctggttcatc gtaatacaca tttgtctgat aagctaggtt cagctttgct tgaacgattg
     1981 gctcacgtac ttgtcgttcc ttcacgatat tcacttgagg ttgggtataa aacagatctg
     2041 gataaactgt ttcccgcgac tcaaatggta attgcccaac tgcttcagct gctttgtccg
     2101 gatcaacatc gccgacaaca aaaatgtcga tttgatcatt tgccatcatt tcttgatagt
     2161 aggacgctag cgaactagca gtgatcttgt ccaaatcttc gactacgccc aaccctggga
     2221 tcttttgttc cgggctgttt tcaaaataaa gttcttgcaa ggcataggaa gcaaaagttt
     2281 gtttatcttc cttcaaactt tccaaatata gacgcagatt gttcttttcc aagtcaaacg
     2341 tcaactgatc aaactgatga tttttaatat ttggataaaa taaaacctct tttaaaaaat
     2401 ctaccgcttg agaaaaaagg tttggatcat tgacatattc accattcaca aaactgatcc
     2461 ctgcgtttag ccaatgtaaa tttccttttt ttctaactga taatccaaag cttgcgccat
     2521 aaagctctgc aagttgcgca cttaactttg tctgatccga ataattcaaa ctatttgttt
     2581 ccaacatgct gctgagaagt gaacgtttcg tgatgacttc ttgttgcaga cgcgcagtaa
     2641 aacggataaa aatccggatc gttttgtatt gttttgttgg taagacatga agattcacgc
     2701 cttctgccaa ttgatacacc atatttatat gctccttttc acagttgatt gcccgcttac
     2761 tcttttaatg ggtcaacctg ccttttttac ttattcctct gcttgaaatt tataagttca
     2821 ggcaattcgc cgttttttct aaatgatgtt acactttata gtatagcata gatgtttttt
     2881 atcaaaagaa acagacggct aaaagcgtat attgtttttt tattttttat ttttcgatat
     2941 actaaagaca aataaaagaa gggagggtca ttcatgatca aagaattcaa agagttcatc
     3001 atgcgtggag atgtcttgga tcttgctgtc ggcgttgtta ttggtagtgc gtttacaagc
     3061 atcgtaaacc aagtggtgca aggcttgatc acaccactaa taggttggtt tgtcgcaatg
     3121 atcacaggta cttctgattt agaaggagca ctttcaattc ttgactggtc gccgacaaaa
     3181 ggcgtcacat ttgcgtttgg taacgttatt agtgctatca ttacatttct gatcactggt
     3241 tttgtcttat tcttagttgt caaagcggca aaccgtgcga agaatttacg ctccaaagaa
     3301 gagcaagtgg aagtggaaga aactcctact gcagaagatt atcttagtga catacgcgat
     3361 cttcttgcca aacaagtggc agaagacaat acacatccag tagagcaaac aaatctttcc
     3421 gatacttcta aataataaag aatctaaaaa aaagaaagcc gattttcagt gtcggctttc
     3481 tttttttaga ttcttaactt gctacttgtt cctttttcat gtcgatatgt ggaatatcat
     3541 cttccaaata cacatctgag attgccttga atccaaacga ctcataaaaa gcttgtaggt
     3601 attcttgtgc gccgatcacg attggttttt ctggaaaatt tttctggatc atgttaatcg
     3661 tttcttgaac aatctttctt cctaaaccat ctttacggtt gtcctgatga acaaggacac
     3721 gtccaaaatg gatactctcc gcttcttcat atactcgcgt gtaggcaagg atcttcccat
     3781 cagtatcttg aaaaaacgta tgccaagctt ctccatcaat gtcatcaact tcttgataag
     3841 gacactgttg ttccacaaca aaaactgcga ttcgttcttt gacgatcgcg taaaactctg
     3901 ctgttgacaa ttcttcaaat ttctttacct gatattccac taagcaaacg ccccctgctt
     3961 ttttattctt agcataccga ttcgcacaca aaaaagcaaa cgaaccttac gaatctcagg
     4021 ctcgtttgct ttttaaactg cttcagttaa aaattcgatc tcaactgtcc gagtaagtac
     4081 atctgaatag ctgtaagata ctcgttcaaa agaattttct tcaggatcca aatcaacaac
     4141 gaatactgaa ggataggttt ctgttaaaac gccgcggcgt tctgtttgac gctttcttcc
     4201 tgtctgagca accaacatga ttgggctgcc gatacgacct tctaaatctt tcttgattga
     4261 agctaaagtt gttggcatac agttcacctc tactatcaac tataccacat atttttaaaa
     4321 ttttcaagtt taccataaaa aaaaaagaaa tgcaataaaa ataaataaga agctcattaa
     4381 gatttttcta aattattcct cattataaaa tttcaagtgt ttttttattt tgagttttac
     4441 tctcccgtaa gcgctcatta cttgcttgtg atccattgct aaacattcag ccatgtctac
     4501 aaaatcaacg ccttgcatat aataatgaaa aattttagct tctatttttg aaaactggat
     4561 ttcgcaattt tcgattgctt cttttaataa aagatgctga attgctaaag gttcttgcaa
     4621 tgtgatctct gctaaaaagt ctggtccttc tgtctctatt ttttggtcga ttgagacttc
     4681 atctttttga tcacgacgct tcagcgcgtt ctgcttcctc aacagagaat agatttgatt
     4741 gcttataacg cttttataat aacttccaaa gctaacacct ttgctttttt cgtaagttct
     4801 caatgcctga tataaagcga tccttgcttc ttgttcccaa tcctcagaaa cataatttct
     4861 taaataatag gtgtttttca aacgtagtaa aagtggaaaa tatttttgat acagggcagc
     4921 aaatgcttct tcgtcaccat tacgtgcagc gcgcaataac tcctcagaca taaaataatt
     4981 cctccataaa ttcgtttatg gaggaatctt atcatcaatg attcatcgaa ctgattagaa
     5041 cgatatgtta tttatttttt ctcattaaat cgtccaattt ttgggacaat ttttttaact
     5101 gctcttcatt ccatggagaa ttacgtcgga aatcaataaa atgcatatct ttattttctt
     5161 gggctattgt tttttccgtt ttttttatcg tcttatataa ttcataagca gaagttcgta
     5221 atgctccttg ggaaaagatc atccactgtt ctgctaaatc gcttgtcgcc actgtcactt
     5281 gtgtcaaacg gtcattcagc tgaccagcga ttctctcgat ataactgtca gccgtttcgt
     5341 cttctttcgt aaaaacaaca tccaactggt atttcttata attttgttgg atgcccggaa
     5401 ctagttgggc atcgaagaca acgatgactt ccattccttc atatttcgca taatttgata
     5461 aacgatgtaa aagagcttct cttgcatccg ccattttgtc ctgcttttta agctggacaa
     5521 gttccggcca cgaaccaatc atgttgtagc cgtctacaat caacagctgt tttttcatgg
     5581 tctactcccc ctgacgttgg gaaaaagcct gatacataag caaaccagca gcaacactgg
     5641 catttaagct ctggacgtgc ccggtcatcg ggatcgtcag caattcatcc atttcttttt
     5701 tcaatcctgg actcattcct ttgccttcat taccaatgac taaagcaatt gatcctttgg
     5761 cattccattt ttggaagttt gtgccggtca tatctgtgcc aaatacccaa aatcctgttt
     5821 ctttgagctg ttgcacagct tgtaccaaat tcgtcacgcg tgcaacaggg acatgttcga
     5881 ctgccccagt ggaagtcttt gttacgatag gtgtgattcc aactgctcgg tgttttggta
     5941 tgatcacacc atcgacgcca cttgcgtctg ctgtccgtaa aatcgaccca aaattgtgtg
     6001 gatcttcgat gctatccaaa atcaaaaaga aggggtcttc ttttttctct tttgttcgtt
     6061 caatcagttg gtcaagggat aaatattcat aaggtgtaat cgcaagaacg actccttgat
     6121 gcacgccgtg atcgctcaaa gtttctaatt tttgtttagg aacccatttg actggcacag
     6181 cttgttcacg tgcgatttct ttcagttctt ctactttttc tcctcgtgaa tcttcttgaa
     6241 gaaagagttt attccctcgc ccttgttgta gcgcttctac tgcggcatgg tgcccaaaga
     6301 cgaaattatc ttctaatgct tcttcatttt gatcagaaac tgactgattt cgtccagttt
     6361 gctttttatt tgaatatcta ttttgttttt tgtcttcaaa tccacgtttc gattgatgtt
     6421 tgtttgttct ttctttaccc atttttttca ccaactttct gaatacacca gcggatcaat
     6481 tcttccatcc gctcggtttg ttttgtcaaa tgcagatagc ccatgagcgc ttcgaaccct
     6541 gttgcgaccc gatacgtcgt aacatcagca ttttttgcgc tcgtatggct tttagcattt
     6601 cttcctctgc gataataaag ttcttcttct tctgtcaaaa gatgttcttc cagcatttgc
     6661 tgaatcaaaa aggcttgtgc attcgcagaa acataattcg ttgccgcacg atgcagctta
     6721 ttgggttttg tttctccttg atcaatcagg tggtcacgaa catagatttc gtaaatcgca
     6781 tctccaacat aagccaaggc taaaccgttc aattgtgtgt aatctctcat gcttctcttc
     6841 tccatcgggt tccttgcggt gtatcttcta ataagatccc tttttctttc aacaaatcac
     6901 gaatttcgtc gcttcggaca aaatcacgat tgcttcttgc ttgatttcgt tcttcgatca
     6961 attgttcaat ctcttcatct aataactctt ctgctgtaaa taaaataccg aagatcgaaa
     7021 gccattgcga gaataaagct tctccttgtg ccaagacagc ggcagaaact ttcggctggt
     7081 ctagataagt gttcagccac ttagcaagct catagaccac tgtgatccca tttgctgcat
     7141 taaaatcatc gtccatttct tcgatgaatc gctgttctaa attttctaac aaagcgattt
     7201 tatccgcatc atctgccaaa ttctctgagg cagtttcttg gcggaatttc aaattgtcaa
     7261 acgtattctt cagtcgttgt aaattggtgg ctgcttcttt catcgtcgtt tcactgtagc
     7321 ggatcggacg acggtattgt gtggtcgcca tgaagaaacg caagacttgt ggatcaacgt
     7381 ttttgatcat ttcatgtaca gtgatgaaat tccctagaga tttactcatt ttttcatcgt
     7441 cttcgccaat cgttacatag ccattgtgca tccagtaatt agcgaatttt ttccctgttt
     7501 tcgcttcact ttgtgcgatt tcattttcat gatgaggaaa ttctaaatct tgcccgccac
     7561 catggatatc gatcgtttct cctaaatgtt ttgttgccat aacggaacat tcaatatgcc
     7621 aaccgggacg tcctttgccc catggtgaat cccaagaaat ctcgccaggt tttgcttgtt
     7681 tccataaagc gaaatctaat gggtcttctt tttgctgctg ttcttcccct gtacgctgac
     7741 ttgctccgac ttccagttca tcgatggatt ggtcacttaa tttcccataa ttttcaaatt
     7801 tacgggtacg gtaatacaca tcacctgaag attcataagc atagcctttt tcaaccaaca
     7861 cttgaataaa tgctaaaata tctggcatat gatccatgac tcttggatgc agtgtagcag
     7921 gttcgacatt caacgctttc gtatcttctt caaatgctcg gatgaaacga tcagcaactt
     7981 ctggtgctgt aatacctaat tcgtttgctg tacggatgat tttatcgtct acatccgtga
     8041 aattcgatac gaaatcgact ttatagcctc gatattcaaa atagcgtcga attgtatcga
     8101 aagcaatggt gctacgagca tttccgatat ggatataatt atacacagtc ggtccgcaga
     8161 catacatacc gacatgtccc ttttgtatag ggatgaattc ttcttttttt ctggtcaatg
     8221 tattgtagat ttttatcatt ctgatctcct cactttttct cctttgattc gaacgacttt
     8281 tgctggtact cctaccgctg tagcgtcgtc tggaacatct ttcaacacaa cggcacctgc
     8341 accgattttg gcgttttttc cgatcgttac cggtcctaat atctgcgcat tcgcagagag
     8401 catggctcct tgtttaacag ttgggtgtct tttacccgtt tcttttcctg tgccgcctaa
     8461 tgttacgcca tggaataaaa tcacatcgtc ctcgatttct gcggtttcgc caatgacgat
     8521 tcccattcca tggtcgatga acacgccagt tccaatcgtt gctcctggat ggatctcaac
     8581 accagttagg aaacgccaga attgagcatt gatcttcgcc aataaaaaga gccggtgctg
     8641 atataaaaaa tgagagaaac gatgccaaaa caaggcatga aaaccagggt atgtcaaaac
     8701 gatctcagct gtcgaacgtg cagccggatc gtttctcttt gctgcatcaa tcgctcgggt
     8761 aaaccaactc atgactttcc actcctttcg taagaatttt atgtaggaaa tcatcttctt
     8821 ataaagaaaa gaagcgcctt ttaaataagt ttccttatca aaagacgctt tccgcgtggt
     8881 tccacttttc ttcataatca atgattatct ctgacagcta acgcctgtta tacgttcttg
     8941 gctactttgt ttcctataaa acagtttttc ctgttttctg tgtttcgcca agaccactcc
     9001 cagaggcatt tcggcaacca ttcattgata ccttgcacca cccggtatct ctcttgtaat
     9061 gatcagctgc ttactttttt ctgttcatcg tgtttagttc agtacttgat ttaaatgtgc
     9121 taatgatttt tcacgaccaa gcaattcgat cgtttcaggc aattctggac catgcatttg
     9181 tcctgataca gctacacgga ttggcatgaa aagatttttt ccttttacgc cagtttcttt
     9241 ttgaacagct ttgatggcag ctttgacagt tggagcatct actacttcca tttgttctag
     9301 ctgtgctttg aatgcgtgga gaacagtagg aactgtttca ccagcaagta cttcttttgc
     9361 tgcttcattc aatacaggat gttcattgaa aaatagcgta gacagatcaa cgatttctgc
     9421 agcatagctc attttaggtt gatacaggct aacgatcttc ttcacccatt cgattgtctt
     9481 ttcatctggg ttttctgcta cacgtccatc agcgattaga taaggcagac acatgtcagt
     9541 aaatgcatct agatccattg ctttgatgta atggttgttt acccattgca gttttttcgc
     9601 atcgaaagct gcgggtgatt tacttaggcg ttttggatcg aacattttga tcaattcttg
     9661 gtgttcgaag atttcgtctt cgcccacagg tgaccagccc aataatgcaa taaagttgaa
     9721 cattgcatct ggtagatagc ctaattcgcg atattgttcg ataaattgca agattgattc
     9781 gtcacgtttg cttagttttt tacctgtttc actgttgatg atcaatgtca tatgtccgaa
     9841 ggttggtggt gtccagccaa atgcttcata gatcatcagt tgttttggtg tattggcaat
     9901 atggtcatct ccacgtaaaa cgtgagtgat tttcatcaaa tgatcatcga ccgcaacagc
     9961 aaaattataa gttggcatac catcgcgttt ttggatgaca aagtcgccac cgatattatc
    10021 ggattcgaac gaaatagcgc ctttcaccat atctgtaaat gcgtattctg tattgcgagg
    10081 cacacggaaa cgtacaacag attctagacc ttgtgcttct ttttctgctt gttcttcagg
    10141 tgtcaagttc gcacatgttc ctgcgtaatg aggcatttct cctcttgcgc gttgtgcttc
    10201 ccgttctgct tctaattctt cttctgtgca atagcatttg tatgctcgat tgctagcaag
    10261 cagctgatca atcagcggtt gatagatttc tttacgttca gattgacggt aagggccgta
    10321 ttctccagga ttttcaggag attcatccca attcattccc aaccaagcaa ggttttccaa
    10381 ctggcttttt tcgccatctt cgatattacg tttttggtca gtatcctcaa tacgaataat
    10441 aaagtctcca tcattgtggc gggcaaacaa ataattgaat aatgcagttc ttgcatttcc
    10501 aatatgcagg tgcccagttg gacttggtgc atagcgtacg cgtactttcg tcattttctt
    10561 cctcttcttt caaactagac tttgactaac agcaaaaatt ccgtcagtta cggcttctca
    10621 aatatcattt atttaaagcc actcagcaca aattttacct tttctaaggt aaatagtaaa
    10681 gccaactggt tgaatttcct taccagttgg ctgaatttct tttttatttt gattgtttag
    10741 tctttttctc tgtctctttt ttattgtcga ttcctcgtcc tgcatgtgca ggtttagcaa
    10801 agatcatgcg tccagcagct gtttgaaggg cacttgttac aacgacttcg atacgttcat
    10861 tcatgtaatg ctgtccatct tctacgacaa ccatcgtgcc gtcatcaagg taggctactc
    10921 cttgttggcg ttctgtcccg gcttttacta ctagaacatt catcgtttca ccagggatga
    10981 cgactggttt tacggcattt gccaacgcat tgatattaag aacaggtaca ttttggaatt
    11041 cagagacttt attcaagtta tagtcattgg ttacaatgac accatctaaa agcttagcca
    11101 atttgatcaa tttgctgtct acttcactga tatcttcaaa gtctccatcg tacatttcga
    11161 cagatatacc ctcttctttt tgcaaggcat ttaggatatc taatccgcga cgaccacgca
    11221 cacgtttcaa actatcccct gagtccgcga tgtattgtag ttcgtaaaga acaaaattag
    11281 ggatcatcaa ggttccttct aagaatccag tctttgtaat atcataaatc cgaccgtcaa
    11341 tgatcacgct tgtatccaag attttatatt tatggaaatt ttctcctgat tttcgttcta
    11401 acagttgact ctcaacttga ctagctgttt cttgctcgat ttttttgctt cgtgaaccaa
    11461 aaactttctt ccattcttcg attcgtgtcg ttcccattct caatcccaga tagcctaaga
    11521 tgatcatcag taaaatcggc aatacactat tgacgaatgg aatttccata ttgtaaagag
    11581 gggtcgagat cacaacacct aaagtcaatc cgatgatcgc accgatcgca ccgaagagaa
    11641 gataagtcaa gctgacctca cttagttttt gttctacttg tttgataata gctgagatat
    11701 gtttggccaa cagcaaagat aataaaaaga aaataagtgc gcctaacagg ctgttggtaa
    11761 atgtattatt cagccatccg tttgactgtt gaccgattgc ctgccaagct gagggtaaaa
    11821 atgaaatacc caagcttgcg ccgacaatga tcatcagcaa agtaatgaca cgtttttgca
    11881 tcgtttttcc tccgtttcga caatcattta ttagttagcg gaacacacgc ttcaacgttt
    11941 cagccaaagt tgctactcca actatctcga ttccttttgg cgcttcccag ttacctaagt
    12001 tgtttttagg taggtatact tttgtgaagc cgagtttttg gacttctttg actcgttgtt
    12061 caatatgact gacacgtctg atttctccag tcagtccaat ttcaccgatg aaacattccg
    12121 ttggctgtgt tcctttttct ttatagcttg aagcaatact gacagcgata gctaaatcaa
    12181 tcgctggttc atttaatttt accccacctg cagcttttaa gtaagcatct tgattctgaa
    12241 gaagcaaccc agctcttttt tccagcactg ccatgatcag agatacccga ttgaaatcaa
    12301 gacctgttgt tgttcgtttg gcatttccga acatagtagg tgtgactaat gcttggatct
    12361 ctaccaaaat tggacgtgtc ccttccattg ccacaacgat agcagagcca gttgcaccgt
    12421 ctaggcgttc ttctaagaaa atctgtgaag gattagcgac ttcttccaat ccatgctcat
    12481 gcatttcaaa aatcccaatc tcattcgttg agccaaatcg gttttttact gctcgcaaaa
    12541 tccgaaaact atgatgttgt tcaccttcaa aatataacac cgtatccacc atatgttcta
    12601 acattcttgg gccggcgatc gagccttctt tggtaacatg accgacgata aaaatggcta
    12661 tcccatttgt tttagcggtt ttgagtagtt ctgcagtggt ttctcttact tggctgacac
    12721 ttcctgccac acttgtgatg tctggctgcg tcatcgtttg gatggaatca atgataacat
    12781 aatcagggga gatatgttcg atggcacgac tgatttcgtt catatctgtt tcagcgtata
    12841 gataaaattc tgtgttgata cttccgaggc gttccgcgcg taattttatc tgctctgcgc
    12901 tttcttcgcc ggatacatat aagacttttc cgcctatggc tgctaattgc tgagaaactt
    12961 gtaacagcaa agtggatttc ccgatacctg gatcgccacc aatcaagacc atagaccctg
    13021 gcaccacacc tccgcctaac acacggttca gttcttctaa ttttgttttg atccgaggtt
    13081 cttttttagg gacgacgtct gcgatttttg ttggttttgc tttttctcct gtcaaacttg
    13141 tccgagttct gcggtccgtt gtatcttgtt cgatttcttc gaccatcgta ttccattttc
    13201 cgcaatttgg gcagcgtccc aagaacttag gcgacacata cccgcaattt tggcaaacaa
    13261 attgtacttt tgattttttt gccattgtac cgcctccttt tatcctaatc atgagacatt
    13321 aaatcaagct taagtatctt taacgtttta tttactggaa gaaccaaatc cgccttgacg
    13381 ttgagcagtc gtttgatctt gatctgcttt taaaaacggc aaaaagatcc cttggccgat
    13441 acgctcgcct tttttgatca ctacatcctt gaaaccaaag ttggtgaatt ggaacatgat
    13501 atggccttca tttgcctcat tattgtaata atcactgtca atcacaccta ctccgtttgt
    13561 caacactaaa aaccgcttca acggattgct tgaacggtta gcaagctgca aaaattcatc
    13621 ttctcccatg taagctttga ttccagtagg cacgagggtt ggtttcaatt gtttttcatt
    13681 ttctgataca tcttttttca atccatctgc gactgtttcc aacccctttt tccagcctgc
    13741 tttccagata cttggaatca cgacctcttc tgctgcttca aaatcatagc cagcagcatg
    13801 atgtgtagcg cgctgcggta gattgatatt ttgatcttta tatttagaaa taacttcaaa
    13861 tcctcttttt ctcaccggtt ttcctcccta aataaactac tcctattcta acatatccga
    13921 ggctgtaaaa gactttcttt acaaattctt caagaaattc tgctatctta aagtaaacca
    13981 ttaattcgtt ctttttccaa acgactgcaa gttgtacaaa aaaactattt tttacatgac
    14041 tttactcgtc aaaacaagta gaatagaacg aaattaatga gtggatattt gattgaggtg
    14101 tcacatgcgt aaagaaatta aaaatgcaaa acgaattatt gtcaaagtag gaacaagtac
    14161 attgatttat cccaatggaa atatcaatct tgcggcaatc gatcagttag cttttgtcct
    14221 ttctgatctt agaaatcggg gaaaggaagt gatcttagtc tcatcaggag ctgtaggcgt
    14281 tggtatgcat aaattacatt tgtcagaacg tccgactttg attcctgaac agcaagctgc
    14341 ggcggctgtt ggtcaagccg aactgatgaa tatctataat caaagattca gtgtatataa
    14401 tcaacagacg gcacagatcc tcgtgacaag agacgtggta gattatcctg aaagtcgtaa
    14461 aaatgtgagg aatacattgg aacagctttt gattatggac acgatcccta tcgtgaacga
    14521 aaacgatact gtagcagtgg atgaattaga tcatctgaca aaatttggcg ataatgatca
    14581 gctgtcagcc atcatggcaa aacttgtcgc ggcagacctt ttgatcgtcc tttctgatat
    14641 cgacgggttt tattcagata atccgactac aaatcccgag gctcagatgt atcgctatat
    14701 cgataaaatc gatgaaaaga ttctggcaca agcaggtggt gctggtagca catttggaac
    14761 aggcggtatg cagagtaaat taaaagccgc ttctaggata tttgaagcaa acagcgcaat
    14821 gattttagca aatggaaaaa atccggcaat tatttttgat attcttgcag gaaaagaagt
    14881 aggtacttta tttaaggagg acttgaaatg actgacttga tgcaattagg acaacaggca
    14941 aaagatagcg cgcaacaact agcacttatg cctacaaacc gcaaaaatga gctacttgtt
    15001 caaatggcac aaaccatcaa aaagaatcaa caagcgatca ttgaagcaaa tcataaagac
    15061 ttagaaaaag cagtcgaaaa caatatcagt gaaacgatgc aagaccgctt gcagctgaca
    15121 cctgagcgaa tcaatgccat ggctacagag atcgaaaaaa ttgcttcgtt agacgatcct
    15181 attggaaaag tagatgagat gtggacgaac accgacggat tgagaatcgg caaaaagcgc
    15241 gtgccactag gcgtgatcgg catcatttac gaatctcgtc caaatgtcac gacagatgca
    15301 gctagtctag cattcaaatc agggaatgcc gttatccttc gcggtggcaa agaagcattc
    15361 ttctcgaatc aattattagt ccaattattg cagcaagtac tggtcagtgc tggcgaatct
    15421 ccttatgcca tccagtttgt cgatgatact tctcatgaaa ctgcaggaca gttgatgcga
    15481 ttgacagaat acttagatgt gctgattcct cgaggcggag caaaattaat ccaacgagtc
    15541 aaagaacagg caacggtacc tatcattgaa acaggtacag gaaataatca cgtatatatc
    15601 gataaagaag cacagttatc tatggcagtg aacatcatcg tcaatgcaaa agcttcccgt
    15661 ccatctgtct gtaacgcggc agaaacattg ctgatccatt ctgagatcgc accttttttc
    15721 ttgccagcta ttgaaaaaga actggtagaa cacggggtct ctttacgtgc ggatgctcgt
    15781 gcgttagaat atctagagac ggctgcttta gcagaagaca cagattggga tactgaatat
    15841 ctagattata tccttgctgt caaagtggtc gattctttgg atgaagcaat tgcacatatc
    15901 aatcggcata atactaaaca ttcagaaacg atcgtgacag atagttatgc ggctagtcaa
    15961 cgtttcttaa atgaagtgga cgcggcggct gtctatgtca atgcctccac tcgttttaca
    16021 gacggatcgg tatttggttt tggtgcagag attggtatct ctactcaaaa gttgcatgcc
    16081 cgtggtccaa tggggttaaa tgaattaact tccacaaaat atatcattta tggcgatgga
    16141 caaatccgcg aataatgttt cgtgtgaaac attattcccg ccttttgcct tttttctgta
    16201 aaggttctat ccaactagaa aggtgtcaat atgtttgaaa ttttaatgat tttttttatt
    16261 taccttattt ctctgaatat cgctgctttt ctgggagtcg gtattttatc ccttttcttt
    16321 caattcaaaa aacgaagcat agggagtcaa cgggaaaaat ggtcgcagta ttttgacaaa
    16381 atcgggcaaa aagggctggt tacacgcttg cacattagtt acatggttgc attatgctta
    16441 ttagcgacaa tcaattacta ttcttttttc gatcattcaa tcgcttatac gattacgcta
    16501 ctgattgctg ggatttttca cttatcttat aaatatcagc tgaataaaaa ccacttgaat
    16561 cggactttcc gctaaaaaat atccgtacga taagtagagt gccttgaaga tcggcaaaaa
    16621 actgccggac tttttagagg ctgtgacaaa gattttgtca cagcctcttt ttccatataa
    16681 acggcgttcg cctaccagct gcctggtaaa tctgtttcct cttcgttaag aagcgaaaat
    16741 ccgtcaaatg tttcgccatc ataataacga agcagctcat ccatggacaa gttcatcgtt
    16801 ttttcttttt cttcttccgc tgattcagcc agtagctgtt cccgatacac ctgatacata
    16861 ccagctattt tttgctgcat cgcttttcta gtttcacctt ccgcattgat tattggcaat
    16921 tctttgatat agccacgata aatcaaccga tcatctgaaa attcaatggg taattcaacg
    16981 agtgtcaacc cgacttcctg aaaatattct tccagcatct attatccccc cattcttttt
    17041 cttaaatgaa ttttagctta cctactgaaa attttccagt ttcattattt tgagaaaaaa
    17101 agggaatgtg acaaaaatcg tgtcacatcc cctttttccg aataaatggt gttcttctgc
    17161 caactcctca atcataagtc acaatattca acaaacatga gaaactgttt tttgaaattg
    17221 ttccttgtga ttcagagtta aacgacagtc tcacaacctc ttggtaaacg gcgttcaatc
    17281 agctgaccct cggtattagc tcaaaaaacg gtaaaatatg agaagctatt tttccgtttt
    17341 gtttctctaa tacttaggat aaaacgctga ttttacaacc tctgttaaac ggtgttcaat
    17401 cagctgtcct tcggtattag ctcaaaaaac ggcaaaatat gaggaactat ttttccgttt
    17461 gtttctctaa tactcaggac aaaacgctga ttttacaacc tcctgataaa cggtgttcaa
    17521 aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa tatgagaagc tatttttgtt
    17581 tggctgttct tatgacttga tgcaggacag ctttttcaca acctctgatt tcttattctt
    17641 cttcatttgt agcaaaataa ttacggtgat aaatgataca acatacactg ataagaacaa
    17701 tggcacctgc aaaataaaac gcatagcttt caccaatatg ttcaccaatc acacctgcaa
    17761 taaatggtgc taaagctcca ccgaaaaagc ggacaaaact atagctggac gaagcaacgt
    17821 ttcgttgcag acctggtatt tccatagcaa tcgtcgtcaa cagtgtattg ataattcctt
    17881 ggaaaaatcc tgctaacaca atgctgatcg gtacaaggac tggcatagaa gttccagctc
    17941 ctaataacag caaacaaata atgaataaga caaatgctgc taaaattgtc cgataagtcg
    18001 taaacttccg ctcaagagcc ggtgcaacga aaatcgaagc aatagccaga agcacacccc
    18061 agccaaaaaa tacaaaaccg acttctaatt cagaatagcc aatcaataaa aatggcgcat
    18121 acgcaagtaa agtaaagaat ccgaaattat ataacaatgc gattagtcca actgaacgaa
    18181 gtttatgatt gcttaacgct ttgacacctt cccaaaggcc tacttttctc tttggtttct
    18241 cagtttcagg taaaagtacc gctagggcta taaaaccgca aaacatcaaa aatgctaccc
    18301 caaaaaatgg ataacgccat gaaccactgc ccaagacccc accgactaga gggccaacag
    18361 ccattccgca gccaagtgct gcttcataca tcatgattgc tttttctgtg ttaccaacta
    18421 aaatactgac gatcgttgct aaagcagtag agacaaataa agcattcccg acaccccagc
    18481 cggcacgtaa cccaatcagc attccgatat tcccagataa tccgccaaga ccagcgaata
    18541 caacgatgat aaacaatcct gtcatcaatg tttttttcaa accgatccga ctagataaga
    18601 aacctgtaaa tagcatcacc acaccagtga ctaacatgta actagtaaat aacagggtcg
    18661 tttcagctgg cgtagcgttt aactgtgcag caatcgtttt taagattggg tctactagac
    18721 ccactcccat aaaagcgatc atacaggtag ctgctacaac tagcgctgaa cttttcccct
    18781 ttgtttcttc atttgtttgt tcttgtatat ctatttgatt ctccaatatt tccatccctt
    18841 tctctctatt agcatcatac atgatttatt ttacatgtca atatgcacat gtaaaacgtg
    18901 gaaactttac tgtgatataa taaagaaaat aagacgagag gtaggatttt tatgaatact
    18961 ttagcctata tattattatg cgtgctttta tccaaaccac gttctgggta tgaattgaag
    19021 cagctgatca caattttttg ggaagcccat cacagtcaga tctatacaac gcttgccaag
    19081 ttagaccagc agggttatgt gaccgttttg gacaaagacg agcactctca aaaaaagatc
    19141 taccacttga ctgatgaagg acgtgctctt gtagaggaat ggatcaaaga ggaaactccc
    19201 gctcctacgc aaaaagatga atttctagca aaaatctatg ctttttcgac gctcgataaa
    19261 acaacagcta ctggactgct attcactaga gaacaacagc tgaacaaaat catgcaaaaa
    19321 aatcaaaaaa aattgaatga cctctcgcta gacaagaaac aagaatttgg acgttatgtt
    19381 gtgatccagc gcagaattat gctttgtcag caggaactcc aatggtgtca gcaagtaaga
    19441 gaacaaatgc agcagttttt tgagtaaggg ataaacagaa cctttctaga aatatctatt
    19501 gaagagcaaa ttatcttcaa taaaagagca atacagttgg aaaagaaaaa gcgatccgaa
    19561 cgacaggcca aaacctatcg ttcggatcac tatttttcta gaactgcatt caagagacta
    19621 tgacaaaaag catgccaagg ccttttttca aataacagtg ttccatcatt cattactttg
    19681 aattgctttt agcacacttc ttgaaagcgg gccaacgatg atcaactgca acggtaaagc
    19741 ggcaatcaca ttcattttcc aagcattcat ataagcagag aataattgat caggtattcc
    19801 ttgttccatg atcacaccga ataaagacat acatgtcacc attcctaaaa ccatcaagca
    19861 tgaaatagtt aagatcattt ggaccttgct ttctggattt actggtagtt taaaggcaat
    19921 ttttttcgcc agcacaccga tgatgaagac atccaaaatg aaagccacca cgaatccagg
    19981 cactagacca atcaacaaac ttgataaatg aagatcttga tgcagccata aattatacgc
    20041 gctcatcccc aagaccatca aaaaacacat cattgttgta aaaattattc cttctttttt
    20101 atttgtaggc aatctgtacc actcctttaa atttgtcacg aaaatcatcg tagcacattt
    20161 aaaaatttct cgtaagtact ttttttattt tctgcccttt tatttcataa acttcatcag
    20221 ccgtacgctc gacaaagtaa gaatcatgag aagtaaacaa aatcgttccc ggatatattt
    20281 gcattagctt ctctaaagct tcgatcgttg ccatatcaat aaaattagtc ggttcatcta
    20341 acagcaacac attacttggc ttagtaaaaa gcaaagcaat cgtcagacgg gtcgcttctc
    20401 ccccacttaa tgtacataac ggtttcgtca aagtttcctg agcaaaccct aagtgattca
    20461 gcaaactacg gacaagcgat tctgaaaact ctgtttgtct catgacaaaa cgaatgatgg
    20521 attcttcaga tgtcatttga taaccctttt gctgatatac ttgaaagctt actttcggag
    20581 agagaaggat tccttctcct tgtttgcgga tatgttctaa taaggttgtc ttacctgaac
    20641 cattttcacc gacaatcgcg atttttttac ctaaagaaaa ctgaaaatca ccatttacca
    20701 gtaatgttct cccacttctt tccaattgga cattttgtcc catgatggga tagcggctgt
    20761 ggagttcaag agattttggt actggaaaac ggatttgttt catctgctgc ggcttggttg
    20821 tttctcctat tttttggagt cttctctcta atgttttcgc attcttttgg atggcttttt
    20881 gtacactgtc tttttgttta gaggaagaca accgatccag tctgatttgt tgctttttct
    20941 ttttactgga cactttttcc aacttttccg cttgtttttt cttttcttgg atcgattttt
    21001 ccaatcgttt tttctctttc atgatcgact ctgcttctcg tagctgagtc tttttctcca
    21061 attccttttg agtgagatag gcactataat tccccgaaaa ctctcggatt tctccgtctt
    21121 ttacttccca gattttcgat gctagctcat ctagaaaata gcgatcatga ctaacaaaaa
    21181 ccagcgtacc ataataataa cgaagctctt caatcagtcg atcaatactt tttctatcta
    21241 gatgggttgt tggctcgtct agaagcatcc cttgttcata agaagaaaga agacgtatca
    21301 actcgatctt tcgttcttcc cctccgctcc atttccgctc tgaagtttct tccagtctta
    21361 actggaaatg actggctaat tccaaattta ggtcctttgc ttcagcgaga taggttaatt
    21421 gaggcaggta gttgaaggtg atctctgttt ggattttccc atgatcggga aaaagttcac
    21481 cggctattag tttgagcaaa gtcgatttac cgacaccatt ttctcctatg atccctatac
    21541 gatcttgctg atagacacga agagaatcaa tagatagttc tagctggttg ccaaagctga
    21601 cttgtagatt tgttatattt actgctaaat tttccatatg ttttacactc ctatttcatg
    21661 taaaacaaca attatcgata ctaaaaaaga gcagaaaaat ctgtttgcaa aaaaactgca
    21721 tcacatcttt tcctactctg aacgaaataa ctatgactaa atcaccaaat ctgtcatagc
    21781 tttcacctat tctactgtcg ttcgaaaaaa tggacatact ccatccatga aattgttgtt
    21841 tatacatgtc ttttgaattt atttagttgt tattcaaaag ttcgaaacgt aatttcatcg
    21901 atgcagtcat ttttccgacc tcctttttta ttaggttacc acacacgatt caaaaaaagc
    21961 aatagctact gaatagtaac tgaaaacctt ttattcgaac atagtgatct tttctggtgc
    22021 taaacgaaca gactcgtaac cttcttctac cgcttttcct attgataaaa taataactgg
    22081 aacataacgg tctttatcca aaccaaatgc ctctgccaac tgatctgctt cgaatccacc
    22141 aattggattt gtttcatagc catgatcacg cgcaactaac atgaattgca tagccgctaa
    22201 gctagaatcg actttgacta catcgttcat ttcttctctt gaaaaactct tgtaataagg
    22261 aatgatcgca gctaattgtt gatctctgac ttcttttggc atcttccctg attcgtaagc
    22321 ttggttgtag atttcttcgc catattcgta acattgcata tcgccaaaaa tcatgaccat
    22381 tgcagaagaa gtatcatttt gtctcgtatt gaaacggatc aaaggacgca agatttcttt
    22441 tgcttcctcg ctttcagcga ccacaaagcg ccatggtttc atattgacag aagaaggtgc
    22501 agtcgtcgct tctttgatca tttccagcat ttcttcgtgt gggattttgt atgtttcatc
    22561 atatacgcga acggatcttc taccagtcat aattgttgaa aaatcatttt tttgttccat
    22621 tattattaac ctctttcgtt tctgttgtta tattgcaaag ttgggtattc aatttatcta
    22681 aaagtgttag aaattgtttc atttctacat cgctgaaagc cggggatagc acggaacctg
    22741 tatctttatg gtccatttca caatgcttga ttttcttctg ggctttttct gttagcgcta
    22801 catacacttc ccggttgttc tgaggatttc tctgacgcga aacatagcca tttgcttcca
    22861 ataccttcag atgacgagta actgctcctt gatcaatctg caacttttct tgcaaactgc
    22921 tttgtagcaa tggattttct tctttcagtc tcatcatgat ctcgtatcta gtaagactta
    22981 taccgatatt ttcttcaaag atctggacga ttttttgatc ggctaatttg atttgatata
    23041 aaaggtgact aatctcttct aaagacataa tttcctcctt tctaataatt gactcgtcaa
    23101 cgattgactt gtcaataata gaataaaaaa agaaaaaagt caaagaatca gctgtgtaat
    23161 aaaaaaaacg ctctcaatag aaaagtatag agaaaagaaa ttttttgtag aatgaacgga
    23221 aaaaatgata aagaaagcac aacgctaaaa ccaagattat tgattcttat caaactatag
    23281 aagatagctc ttctgcagta gataatttta caccgccaaa aatttccgaa atcgcctttt
    23341 gcaagaatcc cgtcgaatat tagaaattgg atcagtatgc ttaaacagta ataaaaaagt
    23401 tatgacaaga tgttgtcaca accttttttg agtaaacggt gttcaaaaac ttgcatctgc
    23461 ggtaataagt tcaaccaaac aaaaaacatg aaaatctgtt tttgtttggc tgttcttact
    23521 acttgatgca gggcagcttt ttcactacct ctttttaata accataaaat cctattcctt
    23581 catatctcca gcccatcggt gttagccaat ctctttcctt agcactcaag gtatagtgat
    23641 gggtaccagt tttttcattt ggattaaata gtcgataaat tggcttggaa ccaccagaat
    23701 aaaaacttag accttcataa cgccaaccga tttttgttaa agagttgatc tcagattgtg
    23761 ccattgtgta atgatggtct ccactgtaag gattgtataa acggtagaca ggttgtccgc
    23821 tgtttggtgc ttgccaagca atcccttcat attcccagcc tacagttggc agataatttt
    23881 tttctccggc attcaatgta tataaatgtt ctcctgtatg acggttatac agacgataca
    23941 tcggagaagt tgaagttggt gctgtatcat actgtgtcag attataggtt tcgattacat
    24001 tgttcaatgt agaaccataa ttaggcgcag tggcataacg acctgttagc caagccgtag
    24061 catctttgta agagttcgta ttgcttttcc atgcaccgga atagtagtaa acgcctggct
    24121 gaaaagaagt cgtcttcaat accgttacgt tatcttgcaa tgattctttg tacgaaggat
    24181 attttctaaa agcggcattc accgtaatcc attgcccatt cacatattcc gaagtaggca
    24241 tcgtaacaga ttggccatta tagcttcctt taatcccaaa tagattgtaa ttaggggcag
    24301 aagccaaagt actgttcccc cagccacttt caacgatggc ttgagcaatc atgactgatg
    24361 catataagtc attggcactt gccaaacttt gtgcagttga ggcaatacta tcaatgaacg
    24421 cttgttgaga attagcagat gagcgagcat aaaaagcata gccagtatag ttttcgaatt
    24481 cagacaaatc tgcttgatgg gtatgtgtca atgtttgccc atctgtcaat tggttcgtga
    24541 tttcttctgc attctctgga ttgttctgag cttctgctga tgtactgtct tcggtttgtg
    24601 tttgttctgt tgtttcagtt gttggttctt cggttgaaat tgtattttct gagacagtag
    24661 ctgactgcaa agtactttct gtatcgtttt caattcctgt ttctgaagaa ctcgatgtct
    24721 gagttgcgac agtttctgtc gattcttcag ctagcactgt tgtgttggca actgctagat
    24781 ttcctaaaag aataacgcta aataataaat tctttgcttt catctttttt ctcctttgaa
    24841 ttatgaaact tttcctaaat aacagtgtaa ctaaaaaagt aaaagcaagt caatgtccat
    24901 atttaaataa catttgtttc tttttaataa atttgctttt tctgcataaa aagacgtaaa
    24961 tgaagaatat tcttctccat tcacgcctta aattgatggt cgtttgaata actatttaac
    25021 aagtaattct ctctggatat tatccgttat ggattatact tcctgtgtcg tcggacggat
    25081 aatcatgttg ctgacagaca tccggtctgg cgtttctatc gcaaatgcta cagcagaagc
    25141 tatatcttca gatgttaatc cccattcttt ttgcgcttca tgaagttcaa gagcagcctt
    25201 tttgtcgctg atggtttgat ataattcagt ttgaacagct cctggagaga tgatcgtaga
    25261 tttgatgtta ttttgccgtt gctcttgacg taatccttcc atgattgcac ggacagcaaa
    25321 ttttgttccg caataaacgg cggaatcagg ataaacaaca tgtcccgcca cagaatcagt
    25381 tgaaatgata tgcccagatt tttgtttttc catgattgga agtacggcgg aaattccatt
    25441 taacacaccc atgatgttga tatcaagcat attttgccat tcatcacggt gaccttcaac
    25501 taatggagcg gttggcatga ttcctgcatt attgtacaat acatcgattc gaccgaactt
    25561 ttccatcgct aaatcgataa ctgcttggac ttgtgcaaaa tctcttacat ctgctgtttg
    25621 aatatatagc tcagcttctg gtaaagattc ttttattgct tttaggcgat cttctcggcg
    25681 tgcagctata actagtttag cccctttttc tgctaatagc ttcgttgttg cttcaccgat
    25741 tccgcttgaa gcgcccatga tgacaatcac tttttctgat aaatcattca ttctattctc
    25801 ctcctgtttt gaaaaatctc ttgcttatac tcactatctc ttatttcatc ttactgttca
    25861 aataatccgt tcgacaggtt tgacgatttt ggcagggatt cctgcaacga tggtattgtc
    25921 tgggacatct ttcgtaacag tgcattcgct gcaacaatgg cattctcgcc aatcgttacg
    25981 cctggcagaa tagttgctcc tgctccgatc caagcatttt tcttgatgtg gattggcttt
    26041 actgacaagc ctcttcgttc ttttggatcg gttagatggt tcaccgtgat gaggttgaca
    26101 cgaggaccaa tcaatactga atcttctaaa cagatgccac ctaagtcaac gaacatcact
    26161 tgacgattga taaagacatt tttccctata ctaatatgtt tgccgaaatc tgtataaaaa
    26221 ggcagcagaa cagtcaccgt atcatctact ttgtcactaa tgatattttc tagccgtttg
    26281 cggatatctg ctggttcatg gtacccagta ttcagcgctg ctaaaggctc ttcgttttcc
    26341 gcttggatct gatggatcac ttcaaagagc ggatccgtta ctcgaatctc tttattgacg
    26401 atttgttttt cgatgaggct tgtcattttg ctccctcctt ttatccttac tttataacgg
    26461 acggcttatg atgtctaata cttataaaac atacttaact attcctttta actatactta
    26521 gtggtatgct tttttaaaaa caggagggaa aaaatggata ttcgtatact gacgtatttt
    26581 ttagcagtag ctagagaaaa aaatatcagc cgagcagcaa aatctctgca tatcacgcaa
    26641 cctactctct caaaacaact gaaagattta gaagaagaac ttggtgtgac acttttcact
    26701 cgaggctccc gagagataca gttgacggat tctggtcgat atctttattc aaaaggaaaa
    26761 gaaatcttgt cgttggtaga aaaaacggcg aacaatttaa caaatgacgc aatcatctct
    26821 ggagaaatct acattggcgg aggcgaaact aaagcaatct ctttcatttc tgatagcctt
    26881 cattcgttga tcaaggcgca tcctgatatc cagtttcatt tatatagcgg gaatgcagat
    26941 gatatcatag acaaactgga tagcggatta cttgattttg gactcgtaat cgaaccgaca
    27001 aacaaacaaa aataccagtc atttcgactg ccagcccaag atcgttgggg acttttgata
    27061 catcggaatc atcctttagc aaagcaaaca gtcatccatc cgcaggaact gaaaactgtt
    27121 cctttgatca tttcccggca aacgtcagtg gacagtcaac tagctgaatg gctaggtttc
    27181 agcatagatc agttatcgat cgtcggaaca tataacttat tgtacaatgc ttctttgatg
    27241 gtcgataacg ggacttttgg tgcattagct ctagatggga tcatcaatac ttatgggacg
    27301 gaaaaagtat tcatcccact cgatccgcca ttacaagctt cactgaatct catttggaaa
    27361 aaagaccagc ctctttctcc tgctgctcgt aaatttttag atgtcctaac ggagaaatac
    27421 agttaagtag agtttttttc cttcgctttc agctttttaa caacgaataa gttaagcaaa
    27481 agatccaatg attaaataac ataagtttct taatttgttc cttcctttta tttctgataa
    27541 ttccagtaaa ttactataat tataaaaaaa gaattgacaa aacataactg tctatataaa
    27601 attgattacg ttaacaataa ttcgtttcat gtgactagat catactaggc atgaaaaatc
    27661 tctgaggaac atggtttccc taggaggttt ttcgtgtcta tttttgtttg gccgcaatca
    27721 actggtctag caaacgtgaa aaccaaatct tcaaaaaggg tgcgcgcatg aaaatcaaaa
    27781 agatctttaa tcaaaatgca gtattggtag atgacggtgg acaggaaaaa gtagctattg
    27841 gaaaagggat tggatttgac agaaaaagaa atgatttgat ttttgatcgt gatgtcgaac
    27901 ggatatttgt catggaacca gaaggtcaaa tcaagttgca aaatttactt aatcagattg
    27961 atgaacgctt tttatttgca gcagaaacga tcattgatca tgcggaaact gttttgatgg
    28021 aaaagttgaa tgaacatgta ttgatcgctt tgaccgacca cattgctttt tctgcggaga
    28081 acattaataa cggtatcgtc atccgcaaca aacttttaag agaaatcgaa gtgctttaca
    28141 gtgatgaatt cagtattgct caatgggcag tagagtatct gacaaaagag cttgggattc
    28201 catatactta tgacgaagca ggatatatcg caatccatat tcacagcgga cgaagcggtc
    28261 ggaacaataa ccatcgcagt atccgtgaag tgacgattgt atctgacatc atccatttag
    28321 tggaatcaga attggaaaca gatatccatt cagaaccatt ttcactgaat tattcaagac
    28381 tggtgaatca tttacgactg cttctacaac gaacccatgc gcaacaatat gctgttttag
    28441 acacggaaat cgtagaaatg gtcaaacgaa aatacccaga aagctataag atttctaaaa
    28501 aaatccgtgt gttgctgacc aaagaatacc agctcgcgat cacgaaagaa gaactgggtt
    28561 acttggccat ccatatcgag cgtttaagaa gcgcaatcgt acaaacaaat gttaacaatc
    28621 atgaggagga aaaaaattga aagcatatct acaacgaatg ggacgttccc ttcaacttcc
    28681 agttgcggtc ttacctgctg ctgctttact agtagggatc ggaaactggt gggcatctta
    28741 tagtaacgac attgtcgctc attttttaca agctggcggt aacgcagttt taggtcaatt
    28801 acctttgctg tttgccgttg ggttagcact agggatgtca gtggacaaat caggtgctgc
    28861 tgccttggca ggtttggtcg catttgaagt tccagtaaat gttttaaaaa ctgattcagt
    28921 agctacttta ttgaatatca aaccagaatc agttgatcca tcttttgctc agatcagtaa
    28981 cgtatttatt ggtattattg ccggattgat tgcggcagct ctttataatc gtttccacga
    29041 agcaaaatta ccgatggctt tatctttctt tagcggcaag cgtctagtgc caatcttggc
    29101 tgcctttgtc atgttgctga tcagtgcagt gctactgctc gtatggcctc cggtttactc
    29161 tgctttagtt tcttttgcta aattcatttt gagtttaggc tggggtggtg caggtcttta
    29221 tggcttcttc aaccgtttac tgatccctac aggattgcat caagcaatga actccgtgtt
    29281 ctggtttgac gtagcaggaa tcaatgatat tggtaaattt ttggcttctg aaggaacaaa
    29341 aggcgtaaca ggaatgtatc aagctggatt tttcccaatc atgatgttcg ggcttccagc
    29401 aggagcatat gctatttaca gaaatgcacg tccagaaaac aaagcaaaaa cagcttcctt
    29461 gatgttagca gcagcttttg cttcattctt tactggtgta acagaaccac ttgaattctc
    29521 atttatgttc gttgcttggc cattatatgt cttgcatgca attttcactg ggatttcttt
    29581 agcagtgagt gcattcttcc attggacagc aggatttgcc tttagtgccg gatttgtcga
    29641 ttacttccta tctttgaaaa atccgattgc gaatcagcca ttgatgttga tcgtccaagg
    29701 attagtaaca gcagtgattt actactttgg ctttaacttt gctatcaaaa aattccatct
    29761 gatgactcct ggccgcgagg aagcagatct ttcagatgac gatactgcaa cgactaattc
    29821 agacaataaa tatgcggcac aagcaagcaa aatctacgct gcattaggcg gagccgacaa
    29881 tgtaacttcg atcgataact gtactacacg ccttcgtttg caagtcaaag atacaggcct
    29941 gatcgatcaa aataaaatca aagccactgg cgtacctggt atgaagatca tcgacggaaa
    30001 aaatgcgcaa gtcatcgtag gtacagaagt acaattcgtt gcagatgaga tggcaaaact
    30061 acatggtggt gctgcaacac gacctgcaca aacaaacacc gtggttaaaa cagaaacatt
    30121 tgccggtgga acgatcgaac aagatattta cgctatcgca aatgggaaac tgattccaat
    30181 cactgaagtt tcagatgatg tattcgcgga aaaaatgatg ggtgacggct atgctgtatt
    30241 accagaaaat ggagaaatct tctctccaat cgctggtacg atcacaaaca ttttcccaac
    30301 gaaacatgct gtcggaatcc aaacagatgc tggtatcgaa gtcttgcttc atatgggcat
    30361 caacactgtt gacttaaaag gcgaaccatt cactttatat gtaaaagaag gtcaaaaagt
    30421 cgcaagaggt caattgattg ctttagttga tcttgccgct attcaatctg caggcaaaaa
    30481 cacggatatg gtcgttgtat tcacaaatgg cgacaaagtc caaagcttag aaatcgaacc
    30541 cgcacgtgat gtaaaagcaa atgataaaat cggctctgtt tccaacaaag cctgagccat
    30601 cgtcttcccc agactttttt atcgataaac aaacaggagc tgagacaaaa atcttgtctc
    30661 agctcctttt tccgaataaa aacggcgttc tcctgccaac tccgcggtaa taagtccagc
    30721 caaacgtaac atatgaagag ctatttttta tttgactctt cttattactt gatgcagaac
    30781 agctttttta caacctctga taaacggtgt tcaaaaacca gtttatcggt atgaagtcaa
    30841 atttatgcaa aaatgtggaa agccattttc acaaaatttt t
//
LOCUS       DAHHZJ010000015        30667 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.015, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000015 DAHHZJ010000000
VERSION     DAHHZJ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.015"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(451..1761)
                     /locus_tag="NBL02_000569"
     CDS             complement(451..1761)
                     /locus_tag="NBL02_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HCD2571279.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="NBL02_000570"
     CDS             complement(1773..3191)
                     /locus_tag="NBL02_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD2571280.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3431..4999)
                     /locus_tag="NBL02_000571"
     CDS             complement(3431..4999)
                     /locus_tag="NBL02_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HCD2571281.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5003..5413)
                     /locus_tag="NBL02_000572"
     CDS             complement(5003..5413)
                     /locus_tag="NBL02_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCD2571282.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5410..6333)
                     /locus_tag="NBL02_000573"
     CDS             complement(5410..6333)
                     /locus_tag="NBL02_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HCD2571283.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6330..7208)
                     /locus_tag="NBL02_000574"
     CDS             complement(6330..7208)
                     /locus_tag="NBL02_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HCD2571284.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7195..8532)
                     /locus_tag="NBL02_000575"
     CDS             complement(7195..8532)
                     /locus_tag="NBL02_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HCD2571285.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8535..9638)
                     /locus_tag="NBL02_000576"
     CDS             complement(8535..9638)
                     /locus_tag="NBL02_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HCD2571286.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9712..11175)
                     /locus_tag="NBL02_000577"
     CDS             complement(9712..11175)
                     /locus_tag="NBL02_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCD2571287.1"
                     /translation="MLALAACGSSSSNSSSETEDGKAKITWLNILHHASPPTDTIVNL
                     IEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAMLKNSSVRNALKSGVFW
                     DVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGLVIRKDWLDNLGLDVP
                     TTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFKLFTSYFGAPNGWAVD
                     DNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQEQFAQGRTGIYTGMV
                     DIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGGLLAFPKSQVKDEAQL
                     KKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDLWQQEVQPLSSSRPSE
                     VTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVGSEIEKIVIDATVQYI
                     MGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11186..11878)
                     /locus_tag="NBL02_000578"
     CDS             complement(11186..11878)
                     /locus_tag="NBL02_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCD2571288.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11900..12874)
                     /locus_tag="NBL02_000579"
     CDS             complement(11900..12874)
                     /locus_tag="NBL02_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCD2571289.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13174..14046
                     /locus_tag="NBL02_000580"
     CDS             13174..14046
                     /locus_tag="NBL02_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCD2571290.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14504..16702)
                     /locus_tag="NBL02_000581"
     CDS             complement(14504..16702)
                     /locus_tag="NBL02_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD2571291.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16934..17926
                     /locus_tag="NBL02_000582"
     CDS             16934..17926
                     /locus_tag="NBL02_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCD2571292.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17986..19407
                     /locus_tag="NBL02_000583"
     CDS             17986..19407
                     /locus_tag="NBL02_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCD2571293.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19400..20899
                     /locus_tag="NBL02_000584"
     CDS             19400..20899
                     /locus_tag="NBL02_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCD2571294.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20938..22332
                     /gene="uxaC"
                     /locus_tag="NBL02_000585"
     CDS             20938..22332
                     /gene="uxaC"
                     /locus_tag="NBL02_000585"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCD2571295.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22491..23174
                     /locus_tag="NBL02_000586"
     CDS             22491..23174
                     /locus_tag="NBL02_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HCD2571296.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23176..24012
                     /locus_tag="NBL02_000587"
     CDS             23176..24012
                     /locus_tag="NBL02_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCD2571297.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            24023..25777
                     /locus_tag="NBL02_000588"
     CDS             24023..25777
                     /locus_tag="NBL02_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2571298.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(26053..26394)
                     /locus_tag="NBL02_000589"
     CDS             complement(26053..26394)
                     /locus_tag="NBL02_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCD2571299.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26462..28633)
                     /locus_tag="NBL02_000590"
     CDS             complement(26462..28633)
                     /locus_tag="NBL02_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HCD2571300.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28802..29701
                     /locus_tag="NBL02_000591"
     CDS             28802..29701
                     /locus_tag="NBL02_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCD2571301.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(29772..30572)
                     /locus_tag="NBL02_000592"
     CDS             complement(29772..30572)
                     /locus_tag="NBL02_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCD2571302.1"
                     /translation="MTIKKKLSIPTTTPEETLDQVFEFFDRYELKGIGIGSFGPIGID
                     KTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTDVNAAAYGELKQGAAQGKNS
                     CIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPDDHYEGTCPYHKDCLEGLAA
                     GPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLTLAPEKIILGGGVMNQDHLL
                     QKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSLLLAEHEHQK"
     gene            complement(30569..>30667)
                     /locus_tag="NBL02_000593"
     CDS             complement(30569..>30667)
                     /locus_tag="NBL02_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCD2571303.1"
                     /translation="KRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT         9159 a         6308 c         5416 g         9784 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaaaa taatctaaaa acattgatgc ataagattga taactttttt tacataacaa
     3361 tatatacaaa caaaaaaatt cactaactag tgaaaataag ttagtgaacc tcatttatta
     3421 aagaattaaa ttacagatga tcttttccat catacttgaa aaactcaaaa tcagcataac
     3481 ttcctctttt tttatttaga tcatgcactg ctatcccgat aaaatttcca gtaaaccctc
     3541 ctgagagtat acacatgttt ttttgcggac ctactttctt ccattcttca gttgatacgt
     3601 ctttcaaatg aaactgtgct gtctctttct ttacagtgac tttcaaaaaa gtcttctttt
     3661 ctgtgagtgg aatcgtccaa tcttctattt gtgctactcc gttttctgat tctatcatac
     3721 gtaggcattt tcccttgtcc tcattccaag taacataaca ataaatataa ttggattcat
     3781 ttaagtacag taccagtcca gccatctgat taaaattatt aggcatgtat tcaaatgctg
     3841 tctccgcatc aaaagtaaaa tccgtttgcc gaatagctaa caaatgatgt tcgaaggtag
     3901 attgaagaga ttcaccggaa atcagacgta aaaaacctgg tctacatttt gtatcacacc
     3961 aatcagcatt tggcaaaatg cgtctggcat tccagttctt ctttagctct ccttcaaatg
     4021 tatccataaa ctgatgatct ttttcttgtt gtacctcttc atttgtctca atcattattt
     4081 cttccatcgg agttgtctgg ctatctttaa gtcttaacca atcgtcggac gtccaataca
     4141 cttcttgaat agcggtttct cttcccaaaa tagccgcacc gtttaatggt ctggtacaaa
     4201 gatatgtcat gtaccatctt cctgacgggg tttgtactaa gctcccatgt cctgtacatt
     4261 gcaatgtaga agcaggtttg tcactagctg tcagcatagg aaaaccagga tcaagctcgt
     4321 aaggaccagt aatattcttt gctcggcaaa ctgtaactga atgtcctctt cctgtaccgc
     4381 cttctgccgt catcaaataa taatacccgt tacgaaaata caaatgtggt gcctccgttt
     4441 tcgctaattc tgtacctgca aatattttat agatgggacc atcaagcttc ttcttctctg
     4501 catcatattc ttgtaatacg ataccgctgg atttattcgg tgtgtccaga cgataatccc
     4561 atagttcatt gacaagccat ttttttcccg tttcaagatc atggaacaaa gaaggatcaa
     4621 agccgttact atttaaataa attggttcgg accaaggacc tgtgatctca gatgcagtga
     4681 tcaaataatt gtggacatct ttaaacggac ggcctgcact tttgacatct gtgtaaacaa
     4741 gataaaacaa tccatcagca taactgatct gtggcgccca tatacttcca tttgtaggat
     4801 ttccttgcaa agatacttga gtggtcaaaa tatccgttgt atgtgaccag ttgactaaat
     4861 ttttcgactc atatactcgg attcctggaa gccattcaaa agaggagaca ataatgtaat
     4921 accgctcttt cactcttaat atattaggat ctggattgaa tcctcttaaa ataggatttt
     4981 tcactttgat agtctgcatg attcaggctc cttttctcat tcttactcga tataaactga
     5041 atgccccagc tgcgaaataa aaaaatgcaa aaggaaacgc aataaactga gtgccaatca
     5101 acaatatgaa tcctgttata aaatacaaac cagtggcagt tgttaagtat tttcctttag
     5161 cagtcaaaaa caatgctact gctaaaaaag caagtgaaat aaacaacaca acggcaaaca
     5221 gaccagtcat gtcctgtaat aatccaaatg tattttctaa agacaaagtt gaatgatcta
     5281 cagcaagcga ctgataactt tttttaaact ctgacgcaga catattactt aaagtaactg
     5341 ctaacccgcc aaagaaaatc aaagatagac tgccaccgac cattcctagc aatagttccc
     5401 attttctttt catttctgaa ccccctgctt ttctaaatgt atccattcag gtcttttgat
     5461 cgatgtttca taattgttat tacgttcaga gaatttgaca gtctgttcat ttttcttgtc
     5521 atcccaatta tcccaaccag ttgggtgaac atgcccacct atcttacacg cttcaaataa
     5581 gacatttgca tacggacgcc acggtcttcc taaatagacg tttctagtat caggttctgc
     5641 agtcaaggta cagctttcaa aacgataccc tgttttttga tttttcggcg tagatgctgc
     5701 tgtgatgtat ccaggatttt tactggctaa gctatggata tgacactgat gaaaaacggc
     5761 atctgcccca ccaaatatga aatccaccgt tccttcaatc cagcaatatt cataggtctg
     5821 acgacaatac tcaggattgg cagaagccgg tgtacaaaaa ggagtaccat ctttttgcaa
     5881 atcaggcaac ggacccgtgc ataaggtatc ctgataacca gatagtcgac agttttttaa
     5941 agatacgcga tctcctgaat tgaataaagc aacagcctgt cctacctttt ctcctggacc
     6001 tgctgtattt tcgatgtgaa gattttctaa tacgacattc gatccttcta agaaaaaagt
     6061 agcacttcgg aaagtcccta actggctacc gtcaagatac tcctgttttg caaaacgatt
     6121 accggagata actactgttc ctgctcccac aagtttaaaa ttggataatc tgctttctat
     6181 atattcatca tatcttcctt cttttactat caagaccttt ggatggctat ccggcaggcc
     6241 tgccaaataa tcgattcctt cttggatagt ttcaaaatcc gtcggggtat ctatagcatt
     6301 ctttccaatt atcagtttgt ctttcatcct cataaatgct cccttatgaa ttgtttccaa
     6361 atatttctca tttcttgtgt ttccatatga ccgcctgtta aagaaaaaga agaaaagttt
     6421 tctggactac ccattgattg atactttttg tccagccctt ttctcagctt cagtacgcct
     6481 tcgtccaagc acatcgtatc gttttttcca actaagctaa gacgagggcg cggagcgatt
     6541 ttttcttgga ttgctaacgt tgtataagct gtcaatagat ttggcacata gtaataaaac
     6601 ccatgatgat ctaggcggcg atgtagcaaa agtatttcga tgtccacctg tgccgctaga
     6661 tcaatacaaa ctttgacacg agaatccaat gctgtcaccc accagcttaa taagcctccc
     6721 atagaaagac caatcgttgc gagccgttga gtatctgtgt cttcccttgt ctccaaataa
     6781 tcgatcaagc tgatcacatc ataaatacgc attccccata acgtttttcc tgttaataaa
     6841 aattgtttga acaactcgct ttcagaaata cctccgcgtt cttcaaatcc ccaagcatca
     6901 atcgaccaaa cagcatatcc cattcctgtc agtacttctg caaaagatgg agaagctaag
     6961 taaggagcac catggatcaa ttcacttttc ccgactgaaa aatctccgcc gtgagaatgc
     7021 aggtaaagga ccggcggaaa cggtccttct ttttctggat aagcatagag ggctggtact
     7081 gtttccaatt catttaagta caattgatag tattcaagca taaaaccgtt ttgtttttct
     7141 cggtataatc gttttccact aggggtatgt ctgctagatg gagcacctaa aaaatcattc
     7201 aactgcataa gcaaagtttt tacgaacaac cagttcatcc atattttctg attgatttga
     7261 ttggaatttc tcaatactga tttctttagt attttctaat agaaatgctg gtccctcgtg
     7321 attttcaatc gacatctggt taaaggaaac cttacccgca aagccaatat aaaatccttg
     7381 tctgctcatt tcctctattc ctgtcatcat agcaggaaca ccgggaactg catcttgggc
     7441 catcgagaca tcgatattgt caaatgtgat ttcttgtatg aattgttccg ctaatccata
     7501 aataaaacct gcagaagcat ggacattcct tgccgtgata ttcgaaaaat ggatccttct
     7561 gaatgctggt gtccgttcat ctatcggata agcctttttt tcccatacat acggttcttt
     7621 ccctcgcgga ccacagaaat aataaagatt cagtataaat ggacacatca cattatccat
     7681 gactatattg gatactcgaa tatcttcaac gatcccgcct cgacctcgtc tagatttcaa
     7741 tcgtattcct cgatcagttt cttggaagat acaatttgaa atggtaatat tacgaatact
     7801 accactcatt tcacttccta atacgacacc gccatgtcca tggaccatcg tgcaatttgt
     7861 gatcgtgata ttttcgcaag cgatcctttc gtacgtatct tcagtccctg ccttgattgc
     7921 aatacagtca tctcccacgt caatatggca attactaata cgcacatttt tgcatgattc
     7981 tggatcaatt ccgtctgtat ttggtgaatc cgctgggttc aagatcgtca agttatcaaa
     8041 cgttgcatta ctgcaaagaa tcgggttgat cgtccaactt ggtgactgga tcaatttaat
     8101 atctttcaca gtaattcgat gacagttatg gaaactcatc aatttaggac gaggataagc
     8161 gagattatct ggttcatttc taaaagtatg ccaccatttt tttccatttc catctaatgt
     8221 accgaaacca gtgacagaga tgttctctac attttgagca tagatacagg aagcataaac
     8281 ttttcgatgg actccttccc aacgtgaatg aacaacagga taatcttttg gatcgtcaga
     8341 gaatttcaga accgctcctg cgcttaaatg aagttcgaca ttgcttttta aaaataaagc
     8401 tcctgttaga aattcacctg ctggaacaac tacggtcccg cctccgtcac ttgctgcagc
     8461 atcaatggct tgttgtattg cttcagtatt caattcatca attgatgcgc caaatgtcaa
     8521 aatatcatac atatttataa tgcctccact tcaatcaatg cttgcaagaa tgcaccaacg
     8581 cctttttgat cattacaaat aattggttca ctgatgtagt aagcatatga accatctcgc
     8641 tgatctgctc cgcctaaccc tgctacttga cagtttttat tcaagttcag ccagccttct
     8701 tttgtttcca gaacaaattc atcaagtaat ccttgatgtg atttcttcac ttgtggaagc
     8761 cattcttctt tgttcagcag tcccaaacgt attcctttag ccattgcgta aacaaacata
     8821 ctactgccgg aagcttccag ataatttcct ttccgttttg tttctgttgt tacttgatac
     8881 catgtcccgc tgttttcttc ttgatatgga agtaatgcag ccagtgtctt ttctagaatt
     8941 tctttcagca aagaacaatc tgtttcttca tgaagtaatt cataggtatc tgctgcagcc
     9001 atcaaatacc agccaatcga tcggctccaa aaatgatggg atagacctgt ttttggatca
     9061 gcccatggtt ggaccttctt ttcatcccat gcatgaatca gcaaatttgt ttttggatcg
     9121 aatagatgtt tataactgat ttcaaactgt ctcaaaacat cttggtaatc tttttcttca
     9181 ctaaaattct ggataaattc agcataaaaa ggagaaccca tatataagcc atccaaccaa
     9241 atttgataag gataaatttc tttatgccaa aaagctcctt cggatgtgcg tggatggttt
     9301 gccaactgct gggaaaggag ttctgctgct tttttatatt tttcttcgcc tgtttctttg
     9361 tacaataaaa agaaaagctt tcctgtattt acatggtcga tattgtattc ttcaacgctg
     9421 tagccacgga tcgttccatc ttcttgcacg aaatagtcga tattgtcttt tatgaaatca
     9481 aaataaattt tttcttgggt tcgttcccaa agtaattgga accctttaag tacgacgcca
     9541 tagtcataag accattttcc tttataccac ttttcttcat ataagcgagg tgttctttcc
     9601 atgatacttt ttgctaattt ctttgaccag ttttccatta tttgctctcc tttaaaaatt
     9661 aaaataggat ttgggacaaa taccaagaag tatttgtccc aatcgtctat attactgtgc
     9721 cgctgctttg tatgcttcct cgtactcttg tgtgattcga tcgcctcctt gtgctttcca
     9781 gtcttctaca gcttttttga atccatcttc gtcaagctgt cccatgatgt attgcaccgt
     9841 agcatcaatg acgatttttt caatttctga tcctacttca ttgttcgtcg gtgaatccaa
     9901 tggcacagaa ggatccatta cagcaaattc ttcattttct ttgatcaatt gattagagaa
     9961 ttctttttca gggtctgtat ctttgaaaga gaacgtcact tcactaggac gagagcttga
    10021 taacggttgc acctcttgtt gccaaagatc catatcgata aattcgatag aaccatcatc
    10081 attcaattca taatgcgttc cttcgatacc gccagtcatc aatttataca catcttcttc
    10141 taaaagatca tcaacaaatt gaagtaattt cttcaattgt gcttcgtctt ttacttgaga
    10201 tttgggaaat gctagcaagc cgccaatacc atttccttct gaccaaacat gataaccgcc
    10261 gccttcacca tctgagatct tgtttacagg cactaattcc atatcatctt gaataccttg
    10321 acccatcgtc ttcagattct tgatatccac cattccagtg tagattccag ttcttccttg
    10381 tgcaaattgt tcttgctgat cagtcttttg ggtaactgca aagtcttgtg ccaaatagcc
    10441 attctcaaat agatctcttg aaaaattcaa agctttcata tactcttctg tatcaaattc
    10501 tggagtgaat ttgccattat cgtctacagc ccagccattt ggtgctccaa aataagaagt
    10561 gaaaagttta aagctggtat aacgaatatc actaggcgca cgatcgccaa aaccaactgt
    10621 gtcgtccaca ccatttccat caggatcgtt ttctgtaaat tgtttagcta cttcataaag
    10681 ctcatccatg gtagttggaa catctagtcc tagattatcc aaccagtctt ttcgaatgac
    10741 taagccagca cgagcgtagt ctttttgtat cggtacgccg tacaaggcac cgtcgatact
    10801 cgcagcttcg attctatctt cagaaatttg acttaaattt ttatagtctt ttaaatattc
    10861 tgatacatcc cagaatacgc ctgattttaa cgcattgcgg acagaagagt ttttcaacat
    10921 cgcaatttga agtgtaacga tatctcctag ttcatcagaa gccaatgcag ttgttaatct
    10981 ttcttctttt gaagcatcag gaatccagtt gaatgtcagt tccgtatcgg ttttttcttc
    11041 gatcaagttc acaatcgtgt ctgtaggcgg tgaagcatga tgcaaaatat ttaaccaagt
    11101 gattttagct tttccatctt ctgtctctga agaactgtta cttgaactgc tgccgcatgc
    11161 ggcaagtgct agaattcctg caaaactaag acctaatttt acgagcgttg cttttttcgt
    11221 aaattttctc cttcactgtt ttaataatat ttttgttttc ttctgttttt tctgtatatg
    11281 agttcttcca ttccaaatgg caaaatatag aatttgctgt ccacgtgact gcggaaaaga
    11341 agagactgcc accgataaag gttaaaacac ctggcattgt ttggatgatc atcagataaa
    11401 tccctaccat acttaatatc aacactaatg agtaatgaag atgtgagaat ccgaataaaa
    11461 atgccatttt cattttgaaa aaatatcctt tccaatcata atgagccatg attgggaaaa
    11521 tatagagtgc tgagagcaca tatatgaacg cagcaatata caaagcgatc ctaagatacc
    11581 aattgtgtat gaaagaaaga tccacaagta aaacaatccc gatggcgcca tatatccagc
    11641 tcatcagcaa ggtttcacga tagttttctt taaaatatgt gaagtacgtt ttcgtcaaag
    11701 gaaacgtttc ttttgagcgc agccattttc taatcactgc aaccatcgca tacgatgcag
    11761 gaccgaaagt aaaaactccc aaacctgcga tgactaaacc gccccacaga aaattcaaat
    11821 aaaccaactg taaaatacga tttagccttc cgtttatttt atctaatgac tgcagcatat
    11881 ctttccctct ctttctatct tagtaaacgc cgtcttctcc tagctttttg gcaatcttat
    11941 tagctaaaat taccataaac aatcctacga cccctttaaa caatcccatt gctgtactga
    12001 aacttaactg tccattcttc aatccagctg tataaatata ggtgtcgaaa atttctgcaa
    12061 cgcttcgatt caatgaattc aataacaagt acatatgctc gaaccctaag tctaatgtct
    12121 gaccgatttt tagaatcagc aaggtaatga ttactggacg aattgccggc aatgtaacat
    12181 gccatgtttt gcgtaagcgt cctgctccat ccatttctgc agcttcatac aactgtgtat
    12241 cgactgcagt tatcgctgaa aggtagatga tcgtagacca gcccaactct ttccagatca
    12301 cttgtccgat atatactgtg cgtgtccatt caggggatgt tagaaaactg attttttcaa
    12361 atcccattgt tgcaaggaat tcattcaata cccctccgtc aacattcaaa aatacataag
    12421 taatcgaaac aatgattacc caagacatga agtgagggat gtaaatgatc gtttgaatcc
    12481 cgtttttcaa tcgtttgttc gttacttcat ttaatagtaa agacaaaatg attggcatcg
    12541 gaaaaaacac gaagatattt aatccgaaca agatcaacgt atttcttaat aatgtaaaga
    12601 atgtcggttc tgtaaacaaa cgaatgaaat gtttgaaacc gacccaagga cttcccatta
    12661 ttcctaaaaa tggttgataa tcttgaaaag caataacgag tccccccata ggaagatacc
    12721 taaaaatcaa gaaataaagg actcccggca gaatcatcac atataaaaat ttattcgttt
    12781 tgattcctgc aattattttt tttcgtcttc tgagcttttc ttcttttttc gatagcgtca
    12841 gttgaaatgg ttcttgtttc tttacacttt ccataagccc ctccttagtt ttgtgaatgt
    12901 ttgctgctta tgacttcatg atagataact atctgaatta cgtatataat catttttacc
    12961 gtaagtaaaa acgcttacat agcaatgatt tttgtgctat acaaaacgat aaagtgtatt
    13021 ttttcacaaa taaactacgc atagcaaacc ttctattttt ttctatctga cacttagcat
    13081 aggaaatgat tatatacgta atctttcacc gatgataaat tgaacatata gaaacagaaa
    13141 catttaattt attttgagga ggagcctatc tatatgaaaa agaaagtctc tataggagaa
    13201 agaagtttta cacttttcaa caatacgttt ctaattttgc tagctttgat ctgtatcgtt
    13261 ccttttttaa atatcatcgc tacatctttt gcttctacac aagaagtcgt cgcgaaaaag
    13321 tttatccttt ttcctactac cttttcttta gacgcgtatc gctacattct ttctacgccg
    13381 accattttta gagcacttgc tgtttcgatt ggtgttacag gtgtcggaac gatcgtcagt
    13441 atgtgtgcaa cttcactgat ggcctacggt ctttccagaa agtatttgtt tggtcgaggt
    13501 ttcgtcaact ttcttgttgt attttctatg ttgttcagcg gggggatgat tccaacgttc
    13561 ttagttgttc gttctctagg gctggtcaat tcttactggg ccatgatcct gcctgtggct
    13621 gttaatgcaa tgaatatgat cattatgaga aactttttcc aagccttgcc ggatagtttg
    13681 gaagaatcag caaaaatgga tggttgcaca gattttggtg tatttttcaa aattatgctg
    13741 ccattagcgt taccgtctat tgctactatc tcgctttttt atgctgttac ttactggaac
    13801 acttatatga ctgctatttt gtatatcaac gattcatcta aatggccgat acagattctt
    13861 ttacgccaaa tcgtcattgt atcaagtggg atgcaagcag aaagtagtgc cgttgatgtg
    13921 attccaccag cacaaacagt taaaatggct gttatcgtta ttgcaacagt gcctatgctg
    13981 attgtttatc cttttgttca gaaatatttt gtcaaaggag cactcgtagg ttctgtcaaa
    14041 gggtgattat gtactgaacg caaaatggta gttgtgtcag aagtggtcag cttcaagaaa
    14101 taagaaggca tttacgaaaa ttgcttctta aatttttgtg aaatttcggc ttatttttcg
    14161 aagaatctac tcctggaaca ccgtgtatca gaggttgtga aagagacgtt cagccttgag
    14221 cgatgataaa aactctcaca aacattgcga cgagtaaccc acaggagcaa taagaagcga
    14281 ttttgaaaaa tagcttccca tatttcacaa aattatgcct tattgtcgat agcctgtctc
    14341 ttgaacgcca tttattcgta aaaaagaact gtgacaagac ttttgtcaca gttctttttg
    14401 cgtgcttagc cttgaaattt aactatccat aaatacaaat actagggtta ttctaacttc
    14461 ttttattgct tgaatcagtc cacaagaaaa acagcctctt gattcacaaa tatttttttc
    14521 gatattctcc cggcgtaatc gattccttct ttttaaagaa acgaataaaa ttttgcggat
    14581 ttcgatattg caatctttct gcaatctctt ttacggagag gttagtctct ttcaaccatt
    14641 tttttgctac ctctaaacga tgattttgaa caaaatcacc aaatttttct cctgtttcct
    14701 ttttaaatac attacttaaa tagttagggt tataatgcaa cctatcagcg atcacatcta
    14761 aagacagatc acgatcatat tctgtgtaga cgatatgcat aatattctcc gacaaatttt
    14821 taaattcatg atcagtgatt gcttgagagc attctgtgat tggcagaact aattgttcta
    14881 aaacaagttt ttctagttct ttgggatgat aattgttcag tgcttttaca taaatttgtt
    14941 tcatgttatc aaagtttttt gaatctactc ccaataactg tcctaattga acgagttcat
    15001 tgatcaagcg gaccgaaact acttcccgac tcaaaggatt tttattttgt gcaaacaatg
    15061 catcaatcaa ttcatgtact aaattcttca cttcctgatt tccagaacga atggcttcaa
    15121 acaatcgatt ttgttcttct atcggatatc taataagtgt tttttcatga gcagcaggaa
    15181 cgatttcctg ataaaaaata attgaacttg gaccagtatt tacacgataa tacagggctt
    15241 cttttgcccg atcaaccgat tgcttgcttt cattcagcgt ttcgaaccgg ttactgatgc
    15301 ctatactgat agttaaattc aagtattctt ttacacgttt ttgaatgagt cggcaatact
    15361 ccatgacttt tttgcttgct tctgcatcat ctttatccat tcgataaata gtggcttgca
    15421 tttcttcatt caaaacgatc ggcagcattc gattttcctt tggcacaatt tccgtgatca
    15481 tgttgttgat cgctaaaagt agcaaatcac gctccccacc gtgactatct tctaacagat
    15541 cgatttgaac caaagctgta taatagcagt cattttgaga actataacca aattgctgca
    15601 accgctgatc tagttctcgc ctttctactc gatttctaaa taaactaagt acaaatagcg
    15661 tttccagctg tggcttttgg gtaactaagt gtgcacttaa cgcttctttt tctcctatga
    15721 tccgactgac tgattgagcg accaaatcta gttctttccc actgaatcct tttgatttaa
    15781 gattcagacg ttcctgtatt tgagaaatcg gacgggcaaa tctgtccgaa aataagtagg
    15841 aaagaagacc aatgagtcct accagtgtta aagacactgc aactagcccg attctcagat
    15901 tgcggatcat tgcgccaatt gcactagagt caacttctaa tctgtaaatc caattattat
    15961 aatctgattt cagtactata tacgttttat ctttttctct tatcactttc agctgattgt
    16021 aatctagatc cacgtccgtt aatacagttg gttgctcttt attttttact gtctgagtag
    16081 taaaaaggtt gccaacttcg ttttcaatag aaagtatgtc ttctccactt tcaccaacaa
    16141 cttgttctat tgtatgtttc tctatcgtag caattcccaa tgcatatttt tctttttcaa
    16201 acattggcaa agaaatcacc atttcgattc ctttgttttt cttttgccaa aataaattat
    16261 ttttgttatt tatgtacttc tgttgatact tttcaatttc ctcttcggtc agttgactca
    16321 acgatccatt gattactccc catttaccag ccaaactgac taaatcataa ttattatcat
    16381 ccataacaat cgtttctata aaatttagtt ctttcctgat ttcagaataa gtggagtaat
    16441 ctgtatatgt cagcggatgc ttgaatatgt ccgagaaatt tttggtcgaa ccatacgtag
    16501 aaaacgaata ttcaatcgtt ctaattttcc attcaacgtt agttttaact tgcctgagca
    16561 aattcttttt gtctgttaaa taactcgatt cgatcgattc ataggtattc tggtaaatga
    16621 ataaactaaa tcctatgaca agtacggctc ctaataaaaa catcggtaaa aatattttat
    16681 aaaatactgt acttttcttc attttcttcc tctcctcctt ttaaaataaa aggctttcat
    16741 ttttctgtaa aattgatact catctaatta aatgagcatt ccccgctttc aagaatagca
    16801 taattaaaaa taaaaatcca agtaattttt aacgtgtgca atcttttcat atcaaagatc
    16861 tattgactta tcgtagttat cgtaatatga tgaatgcgtt aacaaaaagt gaaaaagtaa
    16921 ggaaaatgat ggaatggtaa cgatcaaaga tgtcgctaaa gcagctggcg tttctcatac
    16981 gacagtttct cgtgctttga ataacagcag tttaataaaa aatgaaacaa aacaaaaaat
    17041 acgacaacta gctaaggaaa tgaattattc gccaaactat agtgcaaaag gtttagtgaa
    17101 tcaaaaaaaa tatctaatcg gactattttt ctcaagtttg cataaaggaa cttcatcaaa
    17161 tttcctagca gattccattg caggcatcca ttcagtatta gacacaaatt acagtttatc
    17221 tgttgaaagt atcgatgttg ttgatttaac agaaatcaac ctgcaacgct acgatggact
    17281 gatcgttatg agtcaaagtg atgatgatca gcctttcatc gactatctca aagaaaaaga
    17341 atttcctttc gttgttgtca atcgtcacat caatgatcca gcggttatca acgtcactgc
    17401 cgatgatgcg gccggtgtta tgcaggcagt agattacggt attggatgcg gacatacgaa
    17461 gatcgcttat attggcggaa agaaaaattt tcgttcgaca aaagaacggg aaaaaggggt
    17521 attgaaaagt ctaaaaaaag cgagcatccc tgtcaatcca gcctattttt taacaggaga
    17581 ttacagctta gaaagcggcc tccaaaacat ggaataccta ttatcacttt ctgacctgcc
    17641 tactctggtt ttttgcggca acgacgacat agcgattggt gcattgagag ctgtacattt
    17701 agctggcctc cgtgttcccc agaacatttc tctgatcggg ttcgatgatt ctccagtcgt
    17761 ctcctatctc aatccgccgc ttaccaccgt acgtaagcct ttgaaagaaa tgtgtcaaga
    17821 aggaacaaag cttttactag atttgatcga aggcaggacg atcgaacaat ctaaaattga
    17881 gctctccacc ttgttgatcc ttcgtgagtc gatcaattac ttgtaaacta caagtaattt
    17941 tatataaaaa tgttaacgtg tgcataacac agaaaggaag tttatatgaa aaaactgaca
    18001 aaagaattag tgaaaaaaaa cgaagcacct gtaaaagttc ttcagtttgg tgaaggcaat
    18061 tttatgcgtg gatttattga ttggcagatt cagcagctga ataaccaaca attgtttaaa
    18121 ggtaatgttg caatcattca acctttagaa caaggtcttg gaaatatgat gaaccagcaa
    18181 gataatttat atacggtcat tttacaagga ctcagcaatg gcgaaatcgt tgatacttcc
    18241 gagattatca ccgtagtaga acaaatgatc aatccttatg atgaatggga tgcttatcta
    18301 tctctagcag aaaatgacga cttggcctat atcgtctcca atacaacaga ggcaggaatc
    18361 caattcgtgg agactgataa tttaacagat actcccccac agagcctccc gggcaaactg
    18421 acagcctttt tataccgacg tttcaaatta aacaaagctg gctttaccat cattccttgc
    18481 gaactgattg acagaaacgg cgaacgtcta aaagaaatca ttttacgcta cgcagaaaaa
    18541 tgggatttag aacctgcttt tgttgagtgg ttagaaaaag aaaatgtttt ctgttgtagt
    18601 ttagttgatc gcatcgtccc aggttatcct cgcgaagatg caggtgtttt caatcaacgg
    18661 cataactatg aagatcaatt gatggtcaaa gcagaaccat tcatgctttg ggtgatcgaa
    18721 gggcctaaag aattggagaa tcaactacca ttcaaacagg ctggcttgaa tgtcatcatc
    18781 acagatgata tgactcctta tcgtgaacgt aaggtgcatt tgcttaatgg accgcataca
    18841 gcgatggtcc ctcttgcttt gttggcggaa ttggaaactg tagaagaagt aatgaaagat
    18901 cctttattct ccgcatatgt tgatcagtta ttcaatctag aattgattcc tatgctttct
    18961 ttacctaaag atgaattagc catttatgct gaccaaatca aagaacgttt cttgaatcct
    19021 tttgcccatc acaaattaga agcgatttca ttaaatagtg tttcaaagtt cagcacccgt
    19081 ctgcttccag tgttcaagaa gtatatcgaa gaacaaaatc aggtaccacc gttaatcact
    19141 gtttcactag ctgcgctact tcttatgtat cgaggcgatc aagtaaagcc acatgatgat
    19201 gaaaagacga taagcgaatt tactgatgct tggtctgata atggaacagc tattcctcga
    19261 ttattgcaaa acgctgcgct ttggggagaa gatttgtctc aaataccgaa tgtgacagat
    19321 acagttcaag aaatagctgc aaagatcgat caaaaaggtg tccggtcagt attacaagaa
    19381 atgaaaggaa cgatggtcca tgcttgaaac cctgcaacca acaatgaaat taaatcgacg
    19441 agacagtgtg gtcgtcgcat taacccctat tccaaaacaa acacaactga cgattgatca
    19501 gcaaacgatc accactcttg aagatatccc gcaaggtcat aagattgcat tgggtgattt
    19561 gaaagctgga gacaacgtga tcaaatatgg ctatccaatc ggtcatgtga cgacagacgt
    19621 aactgccggt caatggctgc acactcataa tgtaaaaacc aatctttctg gtgaattgga
    19681 ttatcgatat gaaaaagatg tccatccaag tcattacctt tttgagaacc gaacatttca
    19741 aggttacttg cgaaaaaatg gaaaagtagg gattcgtaat gatttgtttt tagtacctac
    19801 cgttgggtgt gtgaatggca ttgctgaact gatcgtcaaa cagttcaagg aaaaacatcc
    19861 agatctggga cagtttgacc atattacgat tttaaagcat ccttatggct gttcgcagct
    19921 tggaaaagat catcaaaata ctcgagaaat tttagcggac gcagtaaatc atcccaatgc
    19981 cggaggagtt ttagtatttg gattaggttg tgagaacaat acagttcctg aattcaaaaa
    20041 aattttaggt gaatatgatg aagaacgagt gaaatttcta gtggctcaag atgtttacga
    20101 tgagatcgaa caaggtgtgg ctctattaga agagctttta acagcggcgg aaaatgacca
    20161 gcgcatatca gtcccgctct ctaagttgaa tgtcggtttg aaatgcggcg gttctgatgg
    20221 tctatcggga ataactgcta atcctttact tggtgcattt tctgactact tgattgctca
    20281 aggaggcagc acgatcttaa cagaagtgcc agaaatgttt ggcgcagaac aagtattgat
    20341 ggcacgagcg gaaaatgaag aagtttttga ctcaatcgtt gacttgatca atgacttcaa
    20401 acagtatttc ttgtcttacg gggaaccagt ttacgaaaac ccatctcctg gtaataaagc
    20461 aggtgggatc actacattag aagataagtc tttaggctgc acgcaaaaat caggaagctc
    20521 tcctgtcgtt gatgtgctac aatatggcga aaaaatcaga aaaccagggc ttagtttatt
    20581 gcaagctccc gggaatgatc ttgtagctgc ctctgctctt gcttcatccg attgccagtt
    20641 ggtcttgttt accacaggaa gagggactcc gtttggctca tatgtcccaa cggtcaaggt
    20701 atctacgaat acaactcttt ttgaacgcaa aggtcattgg atggatttta atgcaggcgt
    20761 cttattagaa caaccaatgg aagtaatctt agaatcgttt gttcaaaaaa tcatcgctgt
    20821 agctagtgga gaagaaacga aaaatgaaca aaatgacgtt cgagaaattg ccatttttaa
    20881 aaacggagta acactttgat tttcataaaa cagtataaaa agaaagaagg aagatttatg
    20941 tttttatctg atgatttttt attaaaagac gattgggcga aaaagcttta ccattcctac
    21001 gcaaaaaaca tgcccattat tgattatcac tgtcacttag atccaaaaga aatttacgaa
    21061 aacaacaatt tcagcaatct gacagaagct tggttaggag gcgatcatta caaatggcgc
    21121 ttgatgcgag cttgcggcgt gcctgaagaa aaaatcacag gaaatgcttc tgattttgaa
    21181 aaatttttag cttggtgtca aaccgtacca aaaattatcg gaaatccgct ttacagttgg
    21241 tcacatcttg aattgaagcg tttcttcggt attgacttgt tgataaatga agaaaatgca
    21301 gagaaaattt ggacacaagc aaatgaaatt ttggcagctc ctgattttcg ccgtcgggaa
    21361 atcgcaaaaa aatcgaatgt agaagtgatt tgtacaacag atgatcccgc agacgacttg
    21421 cactatcatc agttgcttgc tcatgaagaa ccagatttga aggttctacc ttcattccgt
    21481 ccagataaag cactcaatat tgaaaaagaa ggatttaatg catgggtcac actactggaa
    21541 gaagcagctg accaaacgat caccacctat caagaactga tagaagtctt agggcagcgg
    21601 atagaatact tccatcaaca tggatgtcgt gtttccgacc acgctttaga catcttacgc
    21661 tataaagaag cggatgaaac agtattagaa gagatttttc aaaaagcacg agctaacaaa
    21721 ctattatctt cagatgaaat cgatgcttat cgtacagaaa cactgataca tttgacccac
    21781 ttctatcatt ctcataattg gacgatgcag ctgcacatcc atgcttatcg taactgtaat
    21841 acacaaatgt tggaaaaatt agggcccgat accggctatg atggaatgaa tgatctttct
    21901 ttgactattc ctctgcaaaa attattgaac cgtgcagaag aaacggatca gttgccaaag
    21961 acgatccttt attcattgaa tccgaatgac tatcctgcga ttttagcttt gatgggctgt
    22021 ttccaaaaag aacagaatgg aaaactgcag ttaggttctg gatggtggta caacgatact
    22081 cgagctggta tgcgcgatca attgacacaa tttgctgatg gtagtgcttt ggggaatttt
    22141 gtcggcatgc tgacagattc aagaagtttc ctgtcctaca ctagacatga atatttccgt
    22201 cgtgttcttt gtgaattttt aggcgaaatg gtcatgcgag gagaagcacc tgaagatgaa
    22261 caacttttag gaagtctagt tcaagcgatt agttatacaa atgctaaaaa ttactttggc
    22321 ttttttgcct aattgattaa tagtaacagt aacgaatcaa aaacaggata gtgaaataac
    22381 ggaatccgtt tatccacgtt ttagagaaga agacggacca aatttgctcc gtcttcttct
    22441 cttgctgttc tgcttcattg cctataaacc tttatgaaag aagagataac atgcctacta
    22501 tccatattgc cggtgattct actgcggcag aaaaagatga aaacaagcgt cccgagacag
    22561 gttggggaga aaaaatctct gcttattttt cacccgagat ccagatcaat aaccaagcaa
    22621 agaatggacg cagcaccaaa tcatttatcc aggaaggaag gttagcccgt ctagcttcac
    22681 agtttcagcc gaatgatttc ttatttgtac aatttggaca taacgaccag aaaatccaag
    22741 aagaaagagg tacgcttccc ttcggtagct atttaaaaaa tctgcagata ttttttgaat
    22801 gcgcaaaaaa agcgaacgtc caaatggtct tactgacatc tgtgacgcga cgagattact
    22861 tagaaaacgg aacattgaat ccagatatac ttggagacta tccaaaagcg atgcgtgcat
    22921 ttgctgagaa acaccatatt cctcttttag atgtgttctc aagatcacaa gaattgttcc
    22981 agaccttctc aaaagaagag acaaaaaaat tctacctgca tcttatacct gatacttgta
    23041 aaaattaccc agaaggacta aaagacaata cccactttag tccagaaggc gctgataaag
    23101 ttgcccgaat aattgttgaa ttgataaaag aatcccgtct gcctcttgct aattatttac
    23161 aaaaaggagt ttgagatgtt tccattaaat ttaggcatcc gtgcccacga tttagatgtt
    23221 cgaaatcgtg aagaaatcgt tcaaaaagta tcacagcttc acttgtccca tatccaattt
    23281 gcaccacaaa aagcatttcc tgaacaactt aaattaactg acatgacact tgggagtgct
    23341 gcctattttg gtgaatactt ccaaaaaaac aatatcgaac tttccatttt aggatgctat
    23401 atcaacctat ccagcaaaaa tcaagaagtt cgtgcacaag cattggcaac tttcaaacag
    23461 caaattgctc tgtgctctgc ctatcaagcc aagatgattg ctacggaaac cggtagtgtc
    23521 tctgtcggtt acacaaaaga aaattttact gaagaagctt atcagatcgc cagaaattct
    23581 atcattgaat tagtcgctca tgcagaaaat tttggcgtaa ctgttgctgt tgaagcaggg
    23641 atcaatcatc caatctataa ctatcagtta gctaaacgtt taatcgatga agtagcttca
    23701 ccaaatttaa aaatcatttt agattgtgcg aatttgatgc ataaagaaaa tcatgctgat
    23761 caggaaaaag tgatccgagg tgccttggac aatttacatg gttatattac agcgcttcat
    23821 ctcaaagact atatcatgga aaatggaaag atccgcatgg tacctgttgg caagggctgt
    23881 ttagatttcc gcccggtact gcattatatc aaatatgagc gtcctctaat gtacgccact
    23941 cttgaagcta ctcctgagat ttatgttcct gaagcaatag aacacttgca gtttatttac
    24001 caagattgtt aaggagaaga ttatggagga acgaatacag aaaagttatc aaaaatatca
    24061 tgcaaatgct tttcagacat tgctgcatct ttatcaggga aattattggc gttttgccat
    24121 gtcaggactc ttttttatta tcaaacatct tccatcttgg ttaatgccga tcgctattgc
    24181 caatgtaatc aatgcagctt catcgaaaga ttcttctcag ttgcaggcca tttatttcaa
    24241 cgcttttttg atggcttgtc tgattggtca aaatatattg acgaattatt ttcatgtaaa
    24301 ataccatagc acttcgatcc gtcaagtaga agctggacta agagcgaccc ttatcaagaa
    24361 aatccaagaa ctatctattc cttatcaaaa agaattgcaa tctggacgaa tccagtcaaa
    24421 gatcatccgt gatgtagaag ctatccaaac gttgtcctct caagtatttg tcagcggatt
    24481 aaatatcgct gtcaatttat tcgtagcttt aggtatcacg ctttataaaa gtcctgttgt
    24541 tttcatgttt ttcttattga ctgttcctct cgccgcattc gttactctct ttttccgtaa
    24601 aaggatcaga cagtccaact cagacttcag gcaagaaatg gaagagactt ctgcaaaagt
    24661 gatcgaagtg atcgaattag caccgattgc tcgagctcat gggttagaaa aacacgaaac
    24721 aactcgctta aataagcatt ttactcaaat ctatgaaaaa ggattccact tagatatcct
    24781 gcaatctgtt ttcggatcag tcagctgggc tagttttcag atatttcagc tgatttgttt
    24841 gattttcact gggatattag ctttacaagg gcgcatccaa cccggcgatg tcgtgatgta
    24901 tcagacgtat ttcacgacga tcgtcaattc cgtttctgga atgatcacgc tgattcctac
    24961 gatttcaaaa ggcatggaat ctatcacttc tattggcgaa ctgttgattg ctggcgatgt
    25021 agaaacttat cacggtaaga aaaaaatcca acatttggat ggaaattttc gttttgagga
    25081 tgtttctttt ctttatccgg actcactcaa accgattatc gatcattttt ctctagaggt
    25141 gaaagctgga gaaacaattg cttttgttgg gccatctggc tctggaaaat ctacactcct
    25201 taacttattg attggattta tccagccgac ttcaggaaaa atttttctag atgatcagcc
    25261 tttggatgaa ctggatatgc gaagtgtgcg aaattattta gccgttgttc ctcaaaccac
    25321 tttgttgttc tccgcttcga tcaaagagaa catcacttat ggcttgaaaa acgtatcaaa
    25381 agaacgttta gatgaagtca tcgaagctgc gcaactttct tcactgattg accagcttcc
    25441 tgatggtctg gataccttag taggcgaaca tggcaacaag ctgtctggtg gccaaaagca
    25501 acgtatctcc attgcacgag cgttgatacg tcagccaaaa atcattttgc tagatgaagc
    25561 aacctcagct ttagataacc agtcagagaa aaaaatccag caagccctga attacctgac
    25621 agaaacaccc actactttta ttgtggctca tcggctttca acgatcaaag aggcagataa
    25681 aatcgttgtg atcgatgaag gaaaaatcgt tgaaataggt acttacgaaa aactgttagc
    25741 tcaaaaaggc tatttctacc gcttgaaaaa tgaatgacaa aaaataggag ctatgacaag
    25801 acttttgtcg tagctcctat ttctgaataa acggtgttct cctgccaact cctcggtcac
    25861 aagtcacaat agtcatcaaa catgagaagc tgttttttga aattgttcct tgtaactcag
    25921 agttaaacgg taggctcacg acctcttggt aaatggtgtt caagagacag cctatcggca
    25981 ataagaagca attttgtcat cgctcaaggc cgaacgtctc tttcacaccc tctgttgttt
    26041 cataaaatag ttttattcgc tgtaaaggtc gcgaactggt gtcatgatga tacggttcaa
    26101 gtcattgatg attgtactga agccttgctc tttttgcatc aagtcattga ttacttcttc
    26161 tgattggatt ttcacagcca tttcttgtgc tttcattgca tcttcgtcgc tgaactcttg
    26221 tccactcatt tgtttttctt gtaattcgat ttgcatcgct tggaagtctt tgaacagttt
    26281 gtgtgcttct tcgtttgctt gtactttctc gtacgcttct ttcaacgctt tgaattctgg
    26341 taattcacgg atctcacgtt cgatttgatt cgcgctatcg tagatgtttg acatgttatg
    26401 ccctcttttc ttttagtagt tactctctat tgtaacatgg ggttattcgt tttcaaaggg
    26461 gctactctcc ttgatatttc cgccagaggt ctttagccgc ttcaccaatt ccctgaacgc
    26521 cttctttcac catatcgccg aagtttttca gcccttcttt tgcttgttca ccatattgat
    26581 tcaagttatc ttgccaattt ccagactctc cattagaatt tcctgtgttt ccaacttgat
    26641 ctgcagctac cacttgtcct cctgtggcat aggcatctgc tacactaaat cctgtttttg
    26701 aagtatacgg aaggatgcct tctgccgctg atttgaagac tttcccgaca acatttccac
    26761 ttgtcccttc caaatagtgc agcttgctgg attcttcgta acccatccat gtcgaaatca
    26821 ctacgtctgg cgtgtaaccg atcatccatt ggtcatttgc ttttgtggca tcgaaattag
    26881 tttctgtggt tcctgttttc cctgctatcg tataacctgc aggattcgca gcagccgctg
    26941 ttccatttga aaatgtacca agaagcatgc tggtcatttc atcggctgtt tcctttgata
    27001 tgacctgttt tggttttttg tttgtgttat cgacgatcac cgctccagtc gaatcaacga
    27061 ttttcgtgat aagatgcggc gtatacatct gaccatcatt ggcaaatgca ctgtaagcac
    27121 ccgccatgat caatggagat actccttttt ccagtccacc aagggctaag ccataatact
    27181 tatcagattt tgctaaaggc aggccgaatt cttctgcttt atcatatcct ttttgtaaac
    27241 cgatctcatg caaaagccat acagtaggca aattcaaact ttgtgcaaca gcttgataca
    27301 tcggtacttc tccttggtat gtcccgtcaa aattatgcgc atcgtaataa ctttgcggtt
    27361 cgtctttcaa gatactggaa ggcttgtatc cagcttccag tgctggtgta tatacgctta
    27421 atggtttgat cgttgatcct ggtgaacgac gcatctgtgt agcaaagctg aatcctctaa
    27481 agacatgatc tcctcttcgt cctactagtg cttgtacacc acctgtattc ggatctaaag
    27541 ctactgaagc agattggacc atggcaccat ctgaagcatt tgctgggaat aatgcactgt
    27601 tttgataagt agcttctaat tgcttttgat aatcctgatc taaagctgta tagattttat
    27661 agcctttatt catgacatct tcttcatcaa gaccatactc gttaactgct tcatcgatca
    27721 ctgcatcaaa ataataagga taacgataac cagaatctga attatcgtat gtgtcattaa
    27781 gatacgcgct gatatctgtc tggctttctg cttgttcttg ttcttttgtg atttttccgt
    27841 tttcttccat tacacttagt actgtgtttt tccgatcatt cgcatttttt gggtgatcga
    27901 tcggattata aataccagga cctttcagca ttcctgccaa agtagcagcc tcagacagcg
    27961 taacattcgt tgcatctact ccaaaatact ttctggaagc gtcttggaca ccccataccc
    28021 cgttgccaaa ataggcattg ttaagataca tggtcaagat ttcttctttg ctgtacttct
    28081 tttcgatctc gattgccaaa aataattcct tcgcttttcg atcaaacgtt tggtctaatg
    28141 tcagatacgc atttttggca agctgctgag tgatcgtact tcctccgccc cctgatgttc
    28201 cgccagacaa gaccattcgg actgctgcac gggcaatccc tttgatatca aacccgtgat
    28261 gctcgtaaaa attcctatct tctgtcgaga ttactgcatc ttggatatag ggagaaatcg
    28321 cgtttaattc tacatatgtt cctttttggg cgtacagtgt gccagcttct tcgtttttct
    28381 tatcataaat gactgtcgat tggcttaatc ctgattttaa cgtttcgaca tttgcttgtt
    28441 ttgctaaaat aaagaggtag atacttgtca ctaagacaca gatcaaacca atcagcaaaa
    28501 tcaatttgtt cacatgatat tttttccaga tgcgctttcg ccatcgatgg aattgtccga
    28561 gatatggtct gatccaacgc caaaaactga tcaaagcagc ttttatcttt tctaaaatgc
    28621 tttgaaaatc catttgcttc ttctcctgtc tatttgattc aatcgtctta ttttaccata
    28681 gtgaaagagt gttttcttcc tgcttaagtt gtagttcaat atgttttttt acgttagtgc
    28741 aagtagagta acataagaat gaacagaaaa atgtaagttt tttcttaaat atgttttaaa
    28801 aatggtacaa ttagtctgcg attgcactga aaggagtaca cttgtgaaaa tcacgatcac
    28861 tttacctatg aaccatcctg aaacaacaat tcgcgacttg cttgaaaagg aatggttagt
    28921 tcctagaaag gttcgacact ttttgcgtat acgtaaaaac gtttggatca atcaacaacc
    28981 tgctttgttc catcatgaag taaagggcaa tgaccagatc acattgttat tcgaagaaac
    29041 agattataat tatcaagaag ttcaattagg agatgcgaag cacatccgtg ttttatatga
    29101 agatgagcat ttgattatag tggataagcc tgcaggaatg aagactcatc caaatgaacc
    29161 gacagaaaac agtaccttac tcaatcattt agctgcttat ctcgacacta aacagcaacg
    29221 accttacgtt gtccaccgct tggacaaaga aacaagcggt gccgttttat ttgcaaaaaa
    29281 tccatttgtc ttgccgatac ttggaagaat gttggaacaa aaaatgatct atcggagata
    29341 tcaagccatc gtatggggag aaattgccaa agatatgacc atcaccaata aaattggacg
    29401 agatcgccat gatcggagaa aaagggtcgt agatgataaa aatggtcaga gcgccttgac
    29461 acatgtcact gtcaacaaag tactttctga acaaacacaa gtctattgtg ttttggaaac
    29521 cggacgtaca caccagatca gagttcattt agctcataag ggacatccga tcgttggaga
    29581 ccctctctac caaaatagaa cagctgaacg actgatgctc catgcattag aactgcacat
    29641 gatccatcca tttactcaag aaaaaatagt cggtaaagct ttacctggat tatgggaata
    29701 gaaataaaaa agaacagagt cttatcagca gataatgata agactctgtt ctttttgtgt
    29761 aatttgtctg cttacttttg atgttcgtgt tctgctaaca gcaaactacc gataatccca
    29821 ctttcattcg gcagtcccca acgaacgata tattcactta cttgaggtgt ttccatataa
    29881 ccacccatca attcaacaaa ttgctgacgg attttttgaa ggagatgatc ctgattcatt
    29941 accccaccgc ctaaaataat cttttcaggt gctaacgtca acgtatagtt gatcagtgct
    30001 tgagcaatat aataagcctg aatagcccaa acaggatgat cttgcggcag atcttgccct
    30061 ttgatccctg tgcgtgcctc aatagacggt cctgctgcta atccttctaa acaatcttta
    30121 tgataaggac aagtaccttc ataatggtca tctggatggc gtttcaccca aatatgtccc
    30181 atttcaggat gtgcgatccc tgaaaaaatt tcttcattga tcacgacacc tgcaccgatt
    30241 cctgttccca cggtaaggta aatacaacta ttttttcctt gtgctgctcc ttgcttcaat
    30301 tccccatatg cagcagcatt gacatccgtt gtccaagcag caggaatatt gtagcgttcc
    30361 ttgattgtgc caagaaaatc gaattgtgcc cacccttttt tgggagttgc caaaacgtat
    30421 ccgtaattat cttttgtttt gtcgatacca atcgggccga atgagccaat cccgattccc
    30481 ttcaattcat aacgatcaaa aaattcaaat acctgatcca atgtttcttc tggtgttgtg
    30541 gtagggatac ttaatttttt cttaattgtc aaattaagtt agacagatca tcaatactta
    30601 cgtaactcaa aaatacttcc aaaggtgttc gataatccag tgattttcta ggaatattat
    30661 ttcgttt
//
LOCUS       DAHHZJ010000016        30652 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.016, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000016 DAHHZJ010000000
VERSION     DAHHZJ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.016"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(1..190)
                     /locus_tag="NBL02_000594"
                     /pseudo
     CDS             complement(1..190)
                     /locus_tag="NBL02_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(444..2079)
                     /locus_tag="NBL02_000595"
                     /pseudo
     CDS             complement(444..2079)
                     /locus_tag="NBL02_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2402..2881)
                     /gene="rlmH"
                     /locus_tag="NBL02_000596"
     CDS             complement(2402..2881)
                     /gene="rlmH"
                     /locus_tag="NBL02_000596"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCD2571304.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            3309..4598
                     /locus_tag="NBL02_000597"
     CDS             3309..4598
                     /locus_tag="NBL02_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HCD2571305.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(4637..5242)
                     /locus_tag="NBL02_000598"
     CDS             complement(4637..5242)
                     /locus_tag="NBL02_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HCD2571306.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(5447..5911)
                     /locus_tag="NBL02_000599"
     CDS             complement(5447..5911)
                     /locus_tag="NBL02_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCD2571307.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(5933..6253)
                     /locus_tag="NBL02_000600"
     CDS             complement(5933..6253)
                     /locus_tag="NBL02_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCD2571308.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(6397..7476)
                     /gene="pepA"
                     /locus_tag="NBL02_000601"
     CDS             complement(6397..7476)
                     /gene="pepA"
                     /locus_tag="NBL02_000601"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HCD2571309.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            7676..8026
                     /locus_tag="NBL02_000602"
     CDS             7676..8026
                     /locus_tag="NBL02_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCD2571310.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            8249..9196
                     /locus_tag="NBL02_000603"
     CDS             8249..9196
                     /locus_tag="NBL02_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCD2571311.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9253..10671)
                     /gene="pepV"
                     /locus_tag="NBL02_000604"
     CDS             complement(9253..10671)
                     /gene="pepV"
                     /locus_tag="NBL02_000604"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HCD2571312.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            10820..11665
                     /locus_tag="NBL02_000605"
     CDS             10820..11665
                     /locus_tag="NBL02_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HCD2571313.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            11669..12109
                     /locus_tag="NBL02_000606"
     CDS             11669..12109
                     /locus_tag="NBL02_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCD2571314.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            12159..12353
                     /locus_tag="NBL02_000607"
     CDS             12159..12353
                     /locus_tag="NBL02_000607"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571315.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            12489..13175
                     /locus_tag="NBL02_000608"
     CDS             12489..13175
                     /locus_tag="NBL02_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCD2571316.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(13583..14272)
                     /locus_tag="NBL02_000609"
     CDS             complement(13583..14272)
                     /locus_tag="NBL02_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571317.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(14247..16376)
                     /locus_tag="NBL02_000610"
     CDS             complement(14247..16376)
                     /locus_tag="NBL02_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HCD2571318.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(16424..17761)
                     /locus_tag="NBL02_000611"
     CDS             complement(16424..17761)
                     /locus_tag="NBL02_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HCD2571319.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(18013..18372)
                     /gene="rplT"
                     /locus_tag="NBL02_000612"
     CDS             complement(18013..18372)
                     /gene="rplT"
                     /locus_tag="NBL02_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCD2571320.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(18425..18625)
                     /gene="rpmI"
                     /locus_tag="NBL02_000613"
     CDS             complement(18425..18625)
                     /gene="rpmI"
                     /locus_tag="NBL02_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCD2571321.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(18697..19221)
                     /gene="infC"
                     /locus_tag="NBL02_000614"
     CDS             complement(18697..19221)
                     /gene="infC"
                     /locus_tag="NBL02_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCD2571322.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(19265..19390)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            19541..20173
                     /locus_tag="NBL02_000615"
     CDS             19541..20173
                     /locus_tag="NBL02_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCD2571323.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(20175..21080)
                     /locus_tag="NBL02_000616"
     CDS             complement(20175..21080)
                     /locus_tag="NBL02_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCD2571324.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(21165..21824)
                     /locus_tag="NBL02_000617"
     CDS             complement(21165..21824)
                     /locus_tag="NBL02_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCD2571325.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            22140..23084
                     /gene="mvk"
                     /locus_tag="NBL02_000618"
     CDS             22140..23084
                     /gene="mvk"
                     /locus_tag="NBL02_000618"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HCD2571326.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            23077..24054
                     /gene="mvaD"
                     /locus_tag="NBL02_000619"
     CDS             23077..24054
                     /gene="mvaD"
                     /locus_tag="NBL02_000619"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HCD2571327.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            24067..25152
                     /locus_tag="NBL02_000620"
     CDS             24067..25152
                     /locus_tag="NBL02_000620"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HCD2571328.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            25149..26213
                     /locus_tag="NBL02_000621"
     CDS             25149..26213
                     /locus_tag="NBL02_000621"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCD2571329.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(26462..27265)
                     /locus_tag="NBL02_000622"
     CDS             complement(26462..27265)
                     /locus_tag="NBL02_000622"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HCD2571330.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(27285..28127)
                     /gene="kduI"
                     /locus_tag="NBL02_000623"
     CDS             complement(27285..28127)
                     /gene="kduI"
                     /locus_tag="NBL02_000623"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCD2571331.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(28143..29150)
                     /locus_tag="NBL02_000624"
     CDS             complement(28143..29150)
                     /locus_tag="NBL02_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCD2571332.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(29157..29801)
                     /locus_tag="NBL02_000625"
     CDS             complement(29157..29801)
                     /locus_tag="NBL02_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCD2571333.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(29901..>30652)
                     /locus_tag="NBL02_000626"
     CDS             complement(29901..>30652)
                     /locus_tag="NBL02_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HCD2571334.1"
                     /translation="KKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVLKIL
                     DTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETIHLG
                     VLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLEKHP
                     LIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAFSVS
                     MPKYRLTDETKEQIISALKQTKSEIEQRIQK"
BASE COUNT         9009 a         6306 c         5699 g         9638 t
ORIGIN      
        1 ccttactgtt gataattgct ttcatattag gaattggatc ttcacctggc atatattgca
       61 gattagaagc atgtaatttt ttctcagaaa caaaccactc attggaaaag acatgaatca
      121 aatcatcact tacttgtgta aaatagcgac gtttaatgac aaagatatct tcattgctat
      181 ctagtgtacc ttcatttctt tgtgcttaaa ttcaaagata attttctcaa taaagatttt
      241 tgattccagt aagattcctt tactcctttt acaacatagc gttgtccttt gttttgaaac
      301 atcttcttcc cctctttgcc attaaaaata gcccaattct ctataaagaa gattaagctt
      361 cattattata atataaagtt taaatttggt ttattactta ctttttctta ttctctagta
      421 agctaatatt gaaaaaaatg taatcagatc attattttta tatgttgtaa cttcctgata
      481 agaaaactaa ttaaaagaaa gaggtaacta cataaactaa ttaaaaaagc taacttcata
      541 gttggaggat aaatggaaga aacttcaatg attgtttctc cttgtgttcc ttcaatcgac
      601 actagtccta ttatcaatct ctcttttttg tccatttact tttgcgactg ttccccaata
      661 atcaaataca ggaagcgtta ttttttgtcc tttagattca tgtactgtat agactatttt
      721 cgaatccatc acttgataag gcatagtatg ccattccttg ccaattctta cttcatgatt
      781 ctgcaaatgt tctttgtatt gtaatgcttc cgtgggtgca tagtcataaa gtcctccggg
      841 aaggagtgtc gtgtaacgaa taagattttc ttgattcatt accaagtgat ctctctgagt
      901 gacaacttta tgttcatagt ttgcttttgt tgcagcagat acagccaaca gtaataaaac
      961 aattgccgtt atattaacta cttttctttg cttagctgat gtgctcttca tgaaaacgaa
     1021 cgataaaatc aacgagccaa acagtacttg aaagcctaag attcgccaag gaaattgaat
     1081 catttgaatg ggtgtatttt gtaataaaga ccatggaaaa aatttggtgg ataagagcca
     1141 agagactacc gcactaaggt aaatcacttt ttctttgcga ttaaatcctt taaaaaagaa
     1201 gagtggacag atcaaagaaa taataccaat gattcctacc gtaaaggttg tcaggtcatt
     1261 attcatcgca ccaatcaaac tatccgagac atttaatgct tggtgttgta aggtcgtttt
     1321 atctggctca ttaatcgatt gaaccatgat ttgtcggaac atcggatacc aaaagtatgc
     1381 tgttaaaaga atcgtcattc ctactgcctt tgacaactcc acaatcaacc ggatgctttc
     1441 tttttttctt gtttcactta ctacaaagcc gcctaagact agtaagccaa cggctattac
     1501 actcataaaa gtggaaagaa catgtgtata aatgagtaag ctcatactga ctgctaatgg
     1561 aattgcacat ggctttttct cataaatcag tgcgtaagca taatacataa ctatcggtat
     1621 aaccgtaagt gcaatcgctt cagcaatagc agaacgatga taaatatcaa tcgtacggta
     1681 cccataaaat gtatataaac agctaaatat aaaagccgca tcgattcttt ttaaaaattt
     1741 tagtccataa tgaaacatca gcatgatagt acaaacattc aatagccaaa catataagat
     1801 ataactaacg attaaattat tacttatcca ataaaaaatg actgccggaa aaaaggtcaa
     1861 gtagggataa aaataattca ctccgctccc ataatgatta aacgtcgtaa aattgattgg
     1921 accagaaaaa attgaagata accctttgac tctagcaaaa tggaaggcaa tatcccccga
     1981 tgtagaaata gtatgaacat ttctaaaata attgattgaa taaagtccag caataattgc
     2041 taggagaatg gacaatatta tttttttctt tacttgcatt acttaattat atactccttt
     2101 catggattgc tttatctagt ataacttaaa ttttaaataa atcgttattt atttttactc
     2161 tcacttaaaa aagcactaaa attataagag agggaggtaa tggtctgaac accaagaatt
     2221 gttccaacat cgaattgctt aactaatgag ttcaaagctt aattatgtgg ctcttatatt
     2281 taccgagcgt ggattgcgat ctctttagcg aaacaaacat ctttcataaa aaaaacagcc
     2341 atatccgctt tcttggatat gactatttac tactacttta ctatctttca ttcttcttgt
     2401 ctcacttatg ataaggcgcc cccgcattga tccggaaggc acggtagatt tgctcgacaa
     2461 ggatcagacg catcaattga tgtggatacg tcatttttcc aaaagagatc tgagcattgc
     2521 ttcttttcat gacggcttcg ctgagtccta aagagccgcc gataacaaaa acaaggtggc
     2581 tttttccttg gatacctaat tggtcgattt gtttagcaaa agattcactt gtaggatttt
     2641 tcccttcaat tgctaaggca tagacgtatt cttgttcttt gattttcgct aagatccgtt
     2701 ccccttcttt ttcttttact tgtctcattt gcgcttcgct taaattctcc ggggcttttt
     2761 cgtcaggaac ttcgatcaat tcgatttttg cataggcatt cagccgtttg acatactcat
     2821 tgataccttg gatcaggtat ttttctttta attttcctac tgagataatt ttgatattca
     2881 tgttttctct cctttggtct tcatgctcac tttgtttatt ataatcaatt ttttcgtcta
     2941 tatgtttatt ttggctattt ttgttaaaaa gatttcccca acagttatgc acaacatcca
     3001 caggtttatc cacagaaaac agtgttattt taccttattt catcacattc attaggcaaa
     3061 catacgtttt ataagaattt atccacttta tacacaggct gtggatatgt tttatccctt
     3121 atttttccaa tcctatccgc gtagttttac acagtatctg tggaaaaaga aaatcaaata
     3181 ttttcatctg aaaacaatga tttttcctaa agtttttcat aaaaagttca cagtctcaaa
     3241 gtagactaaa gtcaacaaat aagactatac taacagtaga taaatgaatg taattggagg
     3301 aggaacacat ggatagaaaa aatgtgacac caaagatgaa aaaaagtaaa aataatagcc
     3361 tctggcgtaa gttaggtctg gggcttgttg gagggatcgt tggaggacta ctgacagctg
     3421 gcatttttta tgcagcgatg ggcactggca acaatagttc tactgctaca agcggcaatc
     3481 agaattctgc cggtgaaaca gttgtggaaa atgtcaaagt caatgtagat tctgatatca
     3541 ccaaagcggt agataaagtt caagacgctg tcgtttctgt tatcaactta caaagtcaaa
     3601 accaatcaag cggttttggc ggactattcg ggcagcaaga agaaagtagc agctcagatg
     3661 atagtaattt ggaagcttat agtgaaggaa gcggcgtgat ctacaaaaaa gacgggaata
     3721 ctgcttatgt agtcacgaat aatcacgtag tagatggcca gcaaggtctt gaagtcttga
     3781 tgaaagacgg tacgaaggta aaagctgaat tagttggaac agatgcttat tcagatttag
     3841 cggtgttgaa gatcaattca gacaaagtcg aaacagttgc ctcttttggt gactcaagtg
     3901 ctctgaaggt tggtgagcct gcgattgcga ttggttcccc actaggatct gaatatgcaa
     3961 attctgtcac ttcaggaatc atttcttcct tgaaccgaca agtgacaagt acgaatgaat
     4021 cgaacgaaac agtcaatatc aatgcgatcc aaacagatgc agcaatcaac cctggaaatt
     4081 ctggtggtcc tttagttaat atcgaaggac aagttatcgg tatcaactca agtaagatcg
     4141 caagtacttc tgaatctact tctaatgtaa gcgtagaagg aatgggattt gctattccaa
     4201 gtaacgatgt agtgaatatc atcaatcaac tggaaaaaga cggaaaagtg acacgtccag
     4261 ctctaggtat cacaatggtc gacttatctg ccgtttctac tcagcaacaa gaacaaatct
     4321 tgaagattcc ttcatctgta actaacggtg tcatcgtgac aaatgtccaa acagctacac
     4381 cggctgaaaa agctggattg aaacaatacg acgtcattac taagatcgat gacaaagaag
     4441 tttcttctgg tgtagaactt caatctgctt tgtataagaa aaaagtggga gattctgtca
     4501 aaatcactta ctatgaaggc tcgaaagaaa aaacaactac tgttgaattg acgatcgatc
     4561 aatcggcatt aaaacaaaat caaaatagtg aaaattaata aaaaagaggt tgtgacaaag
     4621 acttgtcaca acctctttat tttggaaatg cttctccgac tacggcatcc tcaggcaata
     4681 ctaagattcc tttttttgct ggagcatttg gcaaatgcaa ttcacgagcg gaacagatca
     4741 ttccatagct atctacccct cgcaaagttc ctggccagat catcattcca tctggcatca
     4801 tagctcctgg ttttgcaacg acaacttttt gtccagcttc gatattgggt gcaccgcaaa
     4861 cgatttgcaa tactgtacca ttgtctactt ctgtctcagt aatcgataaa tggtcagaat
     4921 caggatgtgg gacgcatgtt tttacgtacc cgacaacgat cttcggttca tgatcagctt
     4981 caagctgttc ttcgaatcct gcttctttga tcaatcgatt caatttcgct aattcctcat
     5041 ctgttaagct tacttggccg ttcccttcga ttgttagatg atcggagata tggaaaaagt
     5101 tccaagcaac aacttgacct tgttcatctt tgatccgagc aacattccat ttgcgttcaa
     5161 cagtgttttc tgttccttta tcgtcggcta taataaccat caaggtgtct ccgacgtgtg
     5221 ctttgttata agcaaaaatc atgttctttc ctccattatc ctttgctgag atttatctta
     5281 acaaaaaatt gcattcactg catgaaattt ttttcatgct ttatttccga agaagcctct
     5341 tctggaacac catttattcg taacaaggga ctgtgacaag acttttgtca cagtcccttt
     5401 actgttttag ttaattgatt aaaaaggtcg tacaagttcc acttgcttaa ttacttggct
     5461 gttcttcttc gtcagaaacg atcaagacat cacaaggtga atgacgtatc acgtagctgg
     5521 ctacacttcc tgtcataaag cgttcgactg catttagacc tgactgccca accatgatta
     5581 gatcgatctc atattttttt ggcaatttga ctgccatgga ttcacgaaca gaaccatatg
     5641 caataattcc ttctatattt gtgtaatcaa cagatttccc atattcttta cattcttcga
     5701 tcatttcctt ggctgtttca gtttgctgat cgactagatt ttgatcaata ggtgcatagc
     5761 ccataaagtt ataaaattgc tgatcgatta cgtttgctac atatactttc ccattattac
     5821 ggcgagccac ttctaccgct tttttgaaag cagcaagtgc ttgttcacta ccgtcgatac
     5881 ctactaaaat attcttatat tcttgattca tcttgacccc tcctaacaga tgtcagcctt
     5941 ttgttacact ttctaggaac gattcgattt cctgttttgt tttacgatct ttattcacta
     6001 gacgacctaa ttcttttccg tcttggatca cgacaaaact tgggatacca aaaatgttcc
     6061 attcagcggc tacatctatg tacttgtcac ggtctacttc aataaattgc caatcagaaa
     6121 agtcattttc aatttcaggc atttgcggtt tgataaaacg gcagtctccg caccagccgg
     6181 cagtaaagaa aaatacactc tttccagttt ctacgtagcc tgctaattct tctaaactat
     6241 ttgggataat cattctttgt cctctacttt ctgtttatgt atttttcaat taaattatac
     6301 acctttcttt cgaactaaca aacattgatc ctctttttat aaaaaaaatc tggcaaaaag
     6361 caatcttttg ccagatttta tagtttcact atcgcattat tttccgtata cgatcgtatt
     6421 tactgttgat ttatccaaac ctttcagtgt ttgaatcaac atttctcgtg cagcttcaaa
     6481 atcgcgaata ctgaacatcg tttgatgcgt atggatataa cgtcccacta cgccaatcac
     6541 tgtacttggg attccttcgt tttgtgtatg agcagcacct gcatctgtac cgccttttga
     6601 aacaaagtat tgataaggga tattgtttgt ttcagcgata tccagcaagt actcgcgcaa
     6661 acgaggaagc atgatcaaac ctggatcttg gatacgcatc aatgtacctt ctcctaaatg
     6721 tccgaatgtg ccatttttcg tcactgtatc atctgctgca gagcagtcta ctgcaaagaa
     6781 cagatctggc ttgaatttat tgacagaagc tttagaacca cgcaagccta cttcttcctg
     6841 gacattcgcg cctgcaatca atgtatgacc taatgtttca ttttgcaacg cttctaaagc
     6901 ttctaatacc attgtacagc catagcggtt gtcccatgat ttactgataa tattctttcc
     6961 gttggctgtt ttgatcgttt cagcgtaagg aacgattgtg tctcctggaa ggacaccgta
     7021 actcattgcc tcttctcttg attcaaaccc agcatcgaac aaaatatcgg ttacttctac
     7081 tgatttttgt ccgcttgttc ctcgcaataa atgtggggga acggaagaag aaatacatgg
     7141 ataattctta ccagtagagg ttttaagtgt aaaacgctga gcagaaacaa cgtaaggatt
     7201 ccagccgcct agaggtacga ctttaaaaag cccgttttct tggatttgtg tcagcatgaa
     7261 tccgacttca tccatatgtg ctgcgaccat tacacggggt gcatccgctt cttcatgatg
     7321 gcgcagacca aaaatgccgc ccaaaccatc taactgtaac tcatctacca gcggttccat
     7381 atgactttcc atataagcgc gaacatcttg ttcaaaaccg cttgttcctt gtaattctgt
     7441 taactctttg attcgttgaa atgttttttc ttccatatgc tattcgtcta aatttcagac
     7501 taaactcctc tcagacttat attgatctgc ggataaaata tgattttttc tgcagaccga
     7561 tcgtatttat tatagcttat tttttctggt agcggttatt tatttcaaag aaaatatgag
     7621 agtgtggtaa aataagcaca aggagtaaca agtgagaaga aggaggtatg tccacatgta
     7681 tgaagaaagc gaactaagct attttaaagg cgggttagca gttggaatcg gtctaggatt
     7741 attgagcggg gtagcttcaa cgatttggta tcatcgcaaa aaagtattgt cagcagacca
     7801 agtacttgaa aatatcaagt catcttttct aaaagaaggt gcaatcgaag gttcttggat
     7861 ctcttttgaa aaggaaccta ttaggaaatt cgccgttcgg tcgagtgctt atcggggagg
     7921 tctttctcga atagaagatg gcgaaactgt ttattatgaa tttttagcag atgctcatac
     7981 aggaaccgta ttagaaattt caagaaaaaa aggtgcaaca gattaatacg tcaaaaaagt
     8041 gagagaacga atacgcaccc actattcaaa agagaggtat catgtccttc ttttgtagtg
     8101 ggtggttttt tagatagagc tttccaaaca tatattccag tattttcttt ttaaataaat
     8161 gatatttagc gggaaaaact agcatttcct ttgattatga ggtattttta atttagcttt
     8221 tgttttctat agttaaggag agtgaaaaat ggaacaacaa gaaaaaattg ctgttctgat
     8281 tccttgttac aatgaggaag caacaatttc aacagtcatt gcagacttta aacgagaatt
     8341 accagaagca gatatttatg tctatgataa taattcgacc gatcgaacat acgaattggc
     8401 cgttcaaggt ggagcaatcg taaaaaaaga acctcgtcaa ggaaaaggaa atgtgatccg
     8461 ccaaatgttc tttgatatcg atgccgacta ttatttaatg gtagatggcg atgatactta
     8521 tcctgcagaa gctgtacatg gtttgctcga gaaattacgt tctggtgaag cagatatggt
     8581 cattggtgat cgcctatcaa atggaaccta ctttgatgag aataagagac ctttccacga
     8641 ttttggaaat aaccttgtta gaaatacaat tactagaatg tataaaacaa aaattcttga
     8701 tgtcatgacc ggctatcgag gattcaatcg tatctttgtc aaaagttttc ctattatgag
     8761 ttctggtttt caaatcgaaa cggaattgac gatccatgct ttagataaaa aattcaagtt
     8821 cgttgaaatc ccaatcgatt atcgtgatcg tcctgaaggc agcgaatcaa aattgaatac
     8881 attctctgac ggattcaaag tgatcatgat gattattaaa atgtttaaag attataagcc
     8941 tttgcttttc tttgggattt ggaccatttt cttcttttta tttggattgg tcacggggat
     9001 tcctgtgatt cgcgaatata tgctgacaag ctttattaca cgcatccctt ctgcgatttt
     9061 aagtactgga ttgatgactt tagcacttct ttcattagta acagggttga ttttggatac
     9121 agtcgtcacg aacgctaaga aagaatatga attgaatcta tatcatgttt atcatgaata
     9181 ccacagtaga aagtaaaaaa aggatcaaga ctgcaaaaat aatttgcaat tcttgatcct
     9241 ctttttaatt aattatttcg ccaaacgata gatggcatct gcatagattg ctgttgctct
     9301 gaacaagtca tcaagactca taaattcgtt tgcttgatgc attgtatcag tatatcctgg
     9361 gaacatcgct ccataagcaa cgccacgttt caacaaacgt ccgtaagttc cgccaccgat
     9421 gatttgttcg tagccttttt ctccagtgtg gtcttcatag acttgcaata atgtagccac
     9481 caatggatca tccattggta cgtaatgtgg ttccattacg cgagcacctc gagtaactgt
     9541 tgcgccttct tgccccactg tttgttcgat gtcactttgc aatccgtcta ctgttacacc
     9601 ttttggaaaa cggaaattca tattgatgaa gttcgcttcc tctgtaccat tttcttcaaa
     9661 agcaaatagt ccagcattca ttgtcaattt gcccattttt tcatcttcat aagcgactcc
     9721 tagtttttct ccataaaaat cttcatggac ataagcagct gctacatgga taaattgttt
     9781 agcagatcct aggaattcat agttatctaa gaacaatgcc aagaaagaac ctgcattgat
     9841 accagattgt gggcttgcac catgtgcgcc tttaccaact aattcgattt ttacatacgt
     9901 attgtccgct tcaatcgttc cgcttactgg atttgcttcg atgaattggt aaaatgcttc
     9961 ttccatcgcg atagctgcat ctgcacttgg tacttgaagt gctgcagttg ctgttcctgg
    10021 caccatattt tcgcgaagtc cggaaacaaa agatttcaag acataatctc ctgcattgtc
    10081 gcctttgaag cgtaaagcta aagaaacatt tcctttttca ccattgatga ttgggaattc
    10141 agcatctggt gagaaaccaa aatctggttc ttcttcatgt tcgaaataat aagccatatc
    10201 gccccagccg ctttcttcat cgctgccgac tacgaagcga actttttttg atagtttcaa
    10261 gtcaagttct ttaatgatct tcatcgcata gtaagctgcc atacttggac ctttgtcatc
    10321 gcttgatccg cgtgcaaaga tctttccatc cttgattact ggttcataag gatctgtgtc
    10381 ccagccatct cctgcaggaa cgacatccat gtgtccaaat atccctagcg tttcgtctcc
    10441 ttcgccaaga tcaatatgcc ctgcatagtt atcgacattt tttactttaa atccatcacg
    10501 ttcgccataa gctaacatat gtttcaatgc gtcgcgaggt ccaggtccaa atggtgcatc
    10561 tggtgttact tttgagtcat cgcgctcgct gttgacacgt aaaagatttt tcaagtcttc
    10621 aagcaagtct tctttacgtg cttctacttc tttttgccaa tcaattgtca taaagttaat
    10681 cctccactta tttaagtctt cgtgttcatg ataccacaaa ttacagaaaa aaatagttca
    10741 aaaaatctga caattccttt tcttatctgt ttttctctaa aaaaaatata cgctataatg
    10801 agtaaaagga ggtttttaga tgctgataaa cgaacaacta gtgaaaagag tcatcacacc
    10861 tcgtccaaaa atttcacata aaggaacatt tggacgtatc gttttgattg gcggcaatga
    10921 acaatatggc ggagcaatca ttatgagtgc tttagctgcc gtacacagcg gagcgggtct
    10981 cacgactgta gtaactgatg aaaaaaatcg gagtgctctt cacagtcatc tgccagaagc
    11041 aatgattgta gtttggacgg atactgacca gtttctttcc gtaataaagc aagcagatgt
    11101 actcttgatt ggtcccggat tgggtacttc ttctaagagt ttggaacgtt tcaaacaagt
    11161 actagaaaac caatcaaaag aacaatggct ggtgattgat ggctctgctt taagtttact
    11221 tgctgctaat catcttttgc taccctatcc cgaacaaact gtacttacac ctcatcaaat
    11281 ggagtggcaa cggttgagtt ctctaccgat acaagaacaa cgggataaag aaaacaaaaa
    11341 gaagcaacag gaaatcggtg ctgtgctcgt tttgaaaagt catgagacaa aaatttatgg
    11401 tcaccagcac ttctacacca atcctttagg ttctgctgct atggcaacgg gcggcatggg
    11461 tgacacttta gctgggatga tcacaggatt tttagcccag tttcctcaaa atgaccaaac
    11521 gattgcagca gctatttatc ttcatagttt cattggagac cagctggcaa aagaacaata
    11581 tattgttttg ccgacacaaa tcagcgaagc acttccgaaa tggatgaatt atttttgtaa
    11641 aaaatcaatg gaaaacgagg catgagtcat ggagaaattt acaggttatg tgcgagaacc
    11701 tttttattat gaaacagatc aaatggggat tattcatcac agcaattata tccgctggtt
    11761 tgaagaggct cgggtagcct tattggtaca tctagggttt ccctataaaa aaatcgaaga
    11821 atctgggatc atcatccccg ttctggaagt aaattgcagc tataaagaaa tgatccatta
    11881 cggagaaaaa gtgagcatcc atccaactat ccaaaagtat acggggacac ggttggattt
    11941 tagctacgaa attgtgggaa ctgaagacaa aaaattaaaa actactggca gtagtaaaca
    12001 ctgtttttta ctttctgatt cgcaacgatt agtgaagctg agcaaagtca atccggagtt
    12061 agatcagtta ttccaagcgt acgcttcttt ggatgatgta aattcttaat aaagaccgtc
    12121 taagcatttt tcttgtattg tgtcgagtag ttcgtataat gaaaagaaaa aagcaggtga
    12181 tccccatgta tgtcgttaaa gtaatgcacg gttacatcga taaaactggc tgtcgcactc
    12241 gggaaaaaaa cttagacaat ctgctcattt tcaaagacaa aaaagaatcc gaagcttttg
    12301 ctaaaagaat cggcggtcgt gtcaaaccga tccaagaggt acgaccagat tgattcaata
    12361 tttttctttt cgaggctcta ataggcatgc ctatcagggc tttttttgca gactgtgttt
    12421 ttatgcccac acgatttgta agcgatttat gttataatag attatatctg catagaaagg
    12481 aaggttaaat ggttatcaat aatttgcaac agcaagctta tgaatctata cgtaaacaaa
    12541 tcatttattc cgagttagca cctggaagta aaatctccga taaagtgtta gaagaacaac
    12601 tagctatcgg gcgtacaccg attcgagaag cactgatcca actgcgcaat caagagcttg
    12661 taaaaaccat tccacagtcc ggcacttatg tttcaaaaat cgatatccga tctgcttcat
    12721 tagcccgtta tacgcgggaa aagttggaaa aaccgatcct gcaagaatgc agtgcgaaaa
    12781 tgaccaagca agatcaagct aaactagaaa aaatactaga acaaaccgat caggcagtaa
    12841 tcgctcaaaa taaaaaagct ttttttgatt tggatactga ttttcaccga acttgttatg
    12901 aaattgctgg aaaacgagaa atttgggatt ggctagaaag ttatagtacg cacttgaacc
    12961 gtttcagatg gttacgtttg acgatttcag aattagactg gggtcgagta ttagatgaac
    13021 atcagacaat gcttcaatca atgattgatc ataactttga tgaagtcgga tttctttgca
    13081 cgatgcattt acatatgatt atcgaggagc aagaatacgt gattcataat taccccgatt
    13141 attttgaaga tattcaggac agacccatca aataagaggt tttggaatca gcgttttgac
    13201 ctgagtatta gagaaagaaa cggcaaaata gttcctcata ttttgccgtt ttttttgagc
    13261 taataccgag ggtcagctga ttgcacaccg tttattgaga ggttgtgaaa aagctgccct
    13321 gcatcaagta ataagaacag ccaagtaaaa atagcttctt atgtttttaa cttggctgga
    13381 cttattaccg cagatgcaag cttttgaaca ccgttttcca agaggtcgtg agcctgccgt
    13441 tcatctccga gtcacaagga acaatttcaa aaaacagctt ctcatgtttg ttgaatattt
    13501 tgacttgtga ccgaggagtt gacaggagaa catcgtttat tcggaaaaaa aggactgtga
    13561 cacgactttt gtcacagtct ctttatttgt aatctaaatc atactccgta catctttttt
    13621 ctattttttt tatttcttct aagatccctt ggctacgaat ttcattatat ggccaaagta
    13681 agactgcctc taatacataa ggcgaaaaat cgctttgatt catctctttt gaaaaactca
    13741 tcgctgctat ctcgctcaat gcacgaatgt ttgattgatg tgtataaaaa ggcaaccgcc
    13801 acagcacaat tttttcgctt tgcgtataaa ttcccttaac agaagcttcg atgtggtgaa
    13861 atagttcatg tccaataatc aaagcttgcg ctgtttcttt agagatcgaa gctagtcctg
    13921 aacattttaa tttttctaac ggctccgtca tcaatcgaat cccttcatct tctgcataag
    13981 ttgcaaaagt gattcgttcg tttatttgct ctgattcaca ggaaacgatt ggaacagcca
    14041 tttgttcaca aatttctcct acatcggcaa aagggtactt ttttttgatg ctacatgccg
    14101 tttcgatacc acaatcgatt gattgttgta taagaatttt ctgcttggac aaaggaatct
    14161 ttttccaaaa taaatctcgc cgaaactggt agaatcccca aatttcttcg gggatctgtt
    14221 gtaattcgtt tagtaactct tctaacttat gtttgtttga tatttccggc aacgacgatc
    14281 acttcttcct ccggttccat agtagatact tccagatcca tcaaaagctg tagttgttcc
    14341 agatccgtcg ctgtaaaatc agagactctt gctcctaagc agagataaaa ttctggtcgg
    14401 gcaatcaatt cagtttgata aactgccata tcttcccaaa gttgttcgac agctgtcata
    14461 taattggctg cagttgtttt catacatgaa gcatgtccac gttggacctt gataaatcct
    14521 ttttgatcga tgatccgaag acttccagca ttttttgaac gattctgttc gccgcataca
    14581 taaaagaaag gtgtattttc caataagcaa acttcatttt tatttaatcg catatcctct
    14641 gcagcaagct ctagtgcttc ttctgttgtg atctccttgg ttagatcggt tgctttcact
    14701 tccgtagaac cagtagcgat agccgttact tttgaagtct gtggatcgat ttctacgtgg
    14761 acttcgatcg attctggtgt agcgcctgat tcaatcgctt tattcatcgc ttcatttttc
    14821 aacgatcgta tatcttcttt acttggcgaa gggatgatac gttctacaac atctcggacc
    14881 attgcaagtg ctacaccaat agaagaaatc acttcggcgt tttcaggaat cgaatatttc
    14941 acacccattt tattactaaa gtaagtaatc aatgaggcag cgccgccccc tacacctaca
    15001 agagaaatct gatctttctc caagtgatac ttgtctgcca attctaaaat caccggttca
    15061 atcttagcat aggatttttc catgatctgt tctgcgatgt cttcaaccgt cgtatgacaa
    15121 taatcagcta atgcttggat ggcttttcgt gcagatggga catttccata agaaaaatgt
    15181 tcttcttgca ctaatccaag gacattggct gcacatgtat ttgtaatagt cacttcttct
    15241 ccgtcttcca tcactacctt gacgtaatca gcagggtctc cttctttagg agagaaaaat
    15301 tcaaccttag gacctttgat tttttctgtc tccgtaaata ctgcataatc tagaccggca
    15361 atatgtgcag aacgtggacc gacatcgatg attcctgact gattagcccg taccatcgaa
    15421 ccgcctgcca cacctagaac ccgaacatct aatgaagaga tatatgttgg gtggccacct
    15481 acaattgaat aatcgattgc tggacgtcca tttttaatga ccccgatatt ggttgttgtt
    15541 ccgcctactt caaaataaac accattagac gctctcaagt acatcaaaga gcccatgact
    15601 gatgctgctg gaccggaaag catggttaaa acaggtcgtt ttttcatttc attgatttcc
    15661 attactccgc cgtctccgcg cataatcatc aaagaaacat tgacccctgc ttctcgtacg
    15721 gaatcttctg tagaggttgc cgtatctagc atttttggta aaatagatgc attgattgct
    15781 gctgtccgag ttcttcttgt caatccatat aattttgtga tgtcagaagc catcgttgta
    15841 gggatcgatt ttaccgatgc tgcttcataa acaactcgtt ctggttcccc attatctact
    15901 ccaaatgcca tagaagaaac caagacctcc gctctttctc tttctaaaga agaaatcgtt
    15961 tctgaaacac ggtctacatt taagtgtttg acaggtaaga atgcattgac aatctctatt
    16021 tttttcttat tccctaaatc gatatcgttc aatctagttt gtcgttttgc taaaaaacct
    16081 tctaatccac cttttgccat cccgattacg ccgacttttg ctacgtctcc ttcaatcagt
    16141 gcattagtcg cttgtgtggt agaatgtgca acaaacacaa catcttctgg actgatatta
    16201 ttttccagca aacaattttg aaaagactga acgacacctg cggctactcc acggacatca
    16261 tcgtgggttg ttttcactga agattttccg atgatttcgt gcgtcgcatt atcgattgcg
    16321 actgctttcg tatgtgtacc gccaacatca attcccattc gcacaaaacg tttcattttc
    16381 ttttcctcca ttcatctaat cgtttttatc gacgttttgt ttactaggcg ttcccgtaca
    16441 ataagaacgt gatcaggcat aagatcgctc cgatgatcca accagttggg atggatttct
    16501 tcatatattc tcgagaagtt acttttgtat aacctaatcc ccaagcaacc caagattgtg
    16561 taatatctaa atgctgagga gcaatcgttg tgattgcaaa taacgggaac aaaaaggctg
    16621 gcgacatcgt tggattaaca gctaatacga cagctaaaat agctgagcct gaaccgacta
    16681 gattcattgg accgcggaaa aatcctagtg gtgtcaatat agcaaatacg attgccaaga
    16741 ctaatggtga ttgcggtatc caatctccaa aaatagctga aaaataagga gaagcatacg
    16801 cagctgaatt attgaacatc gctaaggtca tcaagaaacc aaccattggc gcaacatcta
    16861 ctgcgccatc tgtaaataat ttcgcaaact gacgacagat ttctttataa cctccgcgca
    16921 attttccagt aaacagcaat gcccagatac ctgctagaat aaaacctaag atgattggaa
    16981 gatctaaaag aacgacccct aaaactggta ataatacggc taaccatgag atcattggtg
    17041 cgtcgccacc ttcagctggt acgtttgctg ccattgcata gcgtttcttt ttgttcattg
    17101 atatattagc gaccgttagt accacaacta agcttacaat catcccaatc ataccaattt
    17161 ggaaatagtc attataagta tagctttcag ccgctggatt gaaaccagca taaatcgttt
    17221 gatattgttt gaaattgacg atattagcaa agattccagc cataatcgag cccataaatg
    17281 agaacagtgc aaccggtact gaaataccta atgacatcaa gattggcaac acaataactg
    17341 caatagaaat cactggtccg attcctgtca tcgacatgaa tataacagcg gttacaatac
    17401 atagtaagct catcgtgatt cttggcttgt cccctccaag ttcaactacc tttcgaatca
    17461 gggtagctgc gattccgcta tctactagaa cacgcccgaa aaaggcacca aagaagacat
    17521 ttaccaagat cgatttggcg tactcagctg gccccgtcgt atagatatgc gtcaacacat
    17581 caattactcc ttgcccttcc atcgccgggt ttggtgaaaa cgtgtttccg actagcgcta
    17641 atcccatcca tacagtagcc atgatggcaa agccgaccat caagttgtag ccttttacac
    17701 agtaccagac catgaaaaag aatgagataa ctaacaaaat accaataata atatcactca
    17761 tgttattcct ccgtccattt tgtttttctg ataagcaaac taatatatca gtcaaactca
    17821 ttctaactta tgtagttagc gctttcatta aagggtgacc ataaatgttc agttttcaat
    17881 taaaaaacct taattatgct attttagact tatacgttat tgtcctgttt catttgatgg
    17941 aggattgtaa ataaaaaaag aggaaagaac ataacttttc gttatcttct ttcctcttta
    18001 taattgttta gcttatttag ccaaagcttc ttttgcttgg tcagccaatg cagtaaatgc
    18061 tgctgcatcg ttaacagcca agtcagctaa cattttacgg ttgatgtcga tttcagcaac
    18121 tttcaaaccg tgcattaatt ttgagtagct caagccattc atacgagctg ctgcgttgat
    18181 acgagcaatc cacaatttac ggaagtcgcg tttcttttga cgacgatcgc gatatgcgta
    18241 gttgtatgaa ttcattactt gttcttttgc tgttttaaat aaagtgtgtt ttgaaccgta
    18301 gtaacctttt gctaatttta gcactctttt acgacgttta cgagttactg ttccaccttt
    18361 aacacgtgcc atgtataatt cctcctaata aaaataaatc ttcagattct gttatttgta
    18421 cttcttattt catttttgct agttgttggc gaatacgttt gaagtcgcca ctagaaacca
    18481 tgcttgcttt acgcaattga cggcgttgtt tttttgtttt gccgtggaaa cggtgacttg
    18541 taaacgcgcg gaagcgtttc aatccgcctt taccagtacg tttgaaacgt tttgctgatc
    18601 cgcggtgtgt tttttgtttt ggcatgacta tatttcctcc tcaaaatctt tcgttatccg
    18661 actatcggct gtccaacagc cagttagtca ttcaaattac ttactgtcgt ttttcggtgc
    18721 cagcgtcaag aacatgctgc gtccgtccat tttcgctttt tgttccactg ttgcaatatc
    18781 cgcagtttct tcagctaagc gattcaagac tttttgacca atttctttat gggtaatggc
    18841 acggcctttg aaacggatag aagctttcac tttatcgccc ttttccaaga acttacgtgc
    18901 attacgaagt tttgtattga agtcattcaa gtcgatcgta ggacttaagc gaacttcttt
    18961 tacattgatc actttttgtt ttttacgcgc ttcgcggtct ttcttttgtt gttcgaaacg
    19021 gtatttaccg tagtccatga ttcgcgcaac tggtggtttc gcaccagggg caactaacac
    19081 aagatccaag tttgcttgtt ctgcaatatt caatgcttct gcttttgtct taactcctaa
    19141 ttgctcacca tctgaaccga tcaatcgtaa ctctcgtgca cgaatgccgt cgttaaccat
    19201 catatccttt gctatggtca ttcacctcca aatattatga gagaaagaaa agcttaccga
    19261 ataaacggca ataaaaaaac gagttctctt acagaacccg cgcgtacttc acctaataga
    19321 accagtgaaa actatctagc ccagtgacat tatcaactaa ggcgagaagc gggtgcttct
    19381 gcttttattt ctcaaccatc atagtatagc aaaaactaat ctttttgtca atgtttttca
    19441 tacagtttgt cttccttttc tgaaaatgat tagattagca gactttctag cagtgattcc
    19501 cgcttctttg ctatactaaa gaaaaacgag gtgaacccac atgaaaaaaa tcgaacctat
    19561 tccaccttct cttcctcttc ttacaaaggg agagttttat ttagaagatg aggagatctt
    19621 ctctggtctt ctgatcaacg ggggtgacca aagctatctc gatgccaaaa atatggtatt
    19681 aagagagagt catatccaaa aacttacgat gcaaaaaaca gttttacgtc attttgaatg
    19741 cagtaatgtc atttttgaaa aatgtgattt ttccaatctg gagtggattg gtggcagttt
    19801 ccaccaagtc gtgtttcatc aatgtaaatt aacaggtacg aattttgccg aaagttatct
    19861 tcgagactgt actttcactg attgtattgc ttcgatggct tcctttagta gtacaaactt
    19921 gaaagctgtc tccttcgatc attgtcaatt agaagacagc gaattttatg aggtcacttg
    19981 gaaaaacctt tttttgtctg acaatcagtt gaccggttcg aactggtttc gaacttctct
    20041 gaagggatta gactttacga ctaatttctt cacagcaatc gctttatctc aagattcttt
    20101 ggcgggacta atcgtcaacc aagagcaagc acttgtgatc gcccaagccc ttggtttgca
    20161 gatcaaagac tagatcaacg aatccgccgc cacagtgcaa taagcatcca aatagataag
    20221 accaaactga tgatcaaggc gattccccat ccgaaaacgg aatgggtaaa gggtaactgg
    20281 agattcattc cgaagaatcc tgtcacaata gttggaacgg tcaagatcag cgaccagacc
    20341 gtcaaaaatt tcatcgtgtc attcaagttg ttattcagca aattattata agtgccagag
    20401 agctggtcta aaatctgcga agctaagttc gtcatttcaa ctgcttgtcg tgcttcgatc
    20461 agcgcatcgt ccaactcttc tttttccttt tctgacaact tgcgataaat agccaatgcc
    20521 ttgatttgtt ctaaaagtac tacattttgt tttgttcctg taactaagta aaccaaaccg
    20581 atctctaaat ctgaaagttg cagcagattc ttgttcgttg ttttttctct taatttcaga
    20641 ttaagccgct gacgttcact attcacttcc tcgattaaag gaaaaaagga atcagaaatc
    20701 aaaaacaatg tacgaaacaa taaactgtat acactcatgt ctggcattcg ttccaaaagc
    20761 gatcgaatta gtcgaatcac gtactcggta ttcggcgtgg taaatgtaaa caaaccgtct
    20821 tcttttaaaa taaaagcgat aggactagtt tcataatgat tatcgacttt ttgctggtgc
    20881 ggaacatttg atacaagcag taacgcttca tcaaaagaat cgtactctac gcgcgcccgt
    20941 tcatttttat ctagagaata agtcagcatc tcgtcactaa tgccatattc ttcttttaag
    21001 tttttaacag ctagcacatc gttttgctgt acattgatcc aaaaattacg gtcttgatta
    21061 aaatagtgtt tttcccgcat actctatcct cctgacattt gtttccagta taaagaaaaa
    21121 agtcgctcaa ataaaggttt accctttatt tgagcaactg taaatcaaat ggctactttg
    21181 gtttggcgaa tctctgctaa tttttcttcg atcattttca agacttcttc ttttgcttga
    21241 ggatcttcca caaaattata gcggtctcca tcgatttgga tttttggact ttctttgtat
    21301 tcttcatacc attgatcgta acgtctgttc aattctttgt aataatcgta tagacttgga
    21361 tcaaaggaca attgttcata cgaacgacca cgtttttcga tacgctccaa catcgtatca
    21421 aaagatacac gaatatgaac taataaatcc ggatgttttt tatgggcagc atagggaagt
    21481 tcttccatca tgttttccag cagttcatca tacacacgaa cttctgtttc tgttgctctt
    21541 cctagatctg catttagatg gaaaagcagt gaatcttcat aaatcgaacg gtccaataca
    21601 ttattgtcat ctttcattgc ttgtttgatg cttgcaaaac gtttattcaa aaaataaatt
    21661 tgcagcaaaa aagcatactg ttcagggttt gcataaaata agggcaagac ttcattatca
    21721 tcaatcgatt cataaaatgc ctgactatca aagtgttcag ccatcatttc tgttaaactt
    21781 gattttccgg caccaattgt acctgctaaa acgatcacgc tcatttacct ccagctcact
    21841 tcatcactaa atattgtgtt taactccaac ctttttaaat atacccccaa tatctagtag
    21901 tgtcaattgt atttttttct agtcaaagaa gaaaaaaagt caaaaaattc ttaagaaaaa
    21961 agtgtttttt ctttagaaaa cgtattctta gttttacttc tgagatggtt ttagtaaaat
    22021 aaaggaggaa tgttttttga gacccttagt ggacaaaaaa attcgtgaag cagcgcaact
    22081 cgttttgggc gctttatttt ttgcttaaaa atcactttta cgtgagaagg agttagatta
    22141 tggcaaacta tggccaagga gaatcaagcg gaaagatcat attgatgggc gagcacgcgg
    22201 ttgtttatgg agaaccagcg attgcctttc ctttctatgc aacaaaagtc accgcattcc
    22261 ttgaagagct ggatgcaatg gacgatcaac tggtttcttc ctactattca ggaaatttag
    22321 ccgaagctcc tcatgcatta aaaaatatca aaaaattatt cattcactta aaaaaacagc
    22381 atgacatcca aaaaaacttg caactgacca ttgaaagcac gattcctgct gaacgtggaa
    22441 tgggatcaag cgctgcagtc gccacagcag tcactcgtgc tttttatgat tacttagcat
    22501 ttcctttgtc tcgtgaaata ctattagaaa atgtccagct ttcggaaaaa atcgcccatg
    22561 gtaatcctag tggaatcgat gcagccgcta ctagcagctt gcagccgatt tattttacaa
    22621 aagggcatcc tttcgactac ttttctttga acatcgatgc ttttttgatt gtcgctgata
    22681 caggaatcaa aggacaaaca agagaagccg tcaaagatgt cgctcacctc tttgaaaccc
    22741 agcctcatga aactggacaa atgatccaaa aattaggata cttgacgaag caagcaaaac
    22801 aagcaatcat tgaaaattca ccagaaacgt tggcacagac aatggatgaa tcacaatcac
    22861 ttctggaaaa gctgacaatc agcaatgatt ttcttaatct attgatccaa acagcaaaag
    22921 ataccggggc attgggtgcc aaattaactg gcggtggacg cggtggctgt atgattgcat
    22981 tagcacaaac aaaaacaaaa gcccaagaaa tcagtcaagc acttgaagat gcgggtgctg
    23041 ctgaaacttg gatacaagga ttaggagtac atacctatgt ttaaaggcaa agcacgcgca
    23101 tataccaaca ttgctctaat caaatattgg ggtaagaaaa atgaagaact catccttcca
    23161 atgaacaaca gcctttcgtt aacattggat gcattttata cagagacaga agtcatcttt
    23221 tctgatagct atatggtaga tgaattttat ctagatggca ccttgcaaga cgaaaaagca
    23281 acaaaaaaag tcagtcagtt tcttgacctt tttcgtaaag aagccggcct ctcactaaaa
    23341 gcttcagtaa tcagtcagaa tttcgttcct accgcagctg gattagcctc ctctgccagc
    23401 gggctagctg ctttagcagg cgcttgcaat actgctctta agcttggatt agatgatctc
    23461 tctctttcaa gatttgctcg acgcgggtct ggttcagctt gccgaagtat tttcggtggt
    23521 ttcgtcgaat gggaaaaagg ccatgacgac ttaagttctt acgctaagcc agtcccttcc
    23581 gattctttcg aagacgattt agcaatggtt ttcgttttga tcaacgacca gaaaaaagaa
    23641 gtgtccagca gaaatgggat gcgtcggaca gtcgaaacat ccagttttta tcaaggctgg
    23701 ttagattccg ttgaagggga tctatatcaa ttgaaacaag caatcaaaac aaaagatttc
    23761 caacttctcg gagaaacgat ggaaagaaac ggactaaaaa tgcatggaac gactttagct
    23821 gcccagcctc cattcactta ctggtctcct gacagcttaa aagcgatgga tgcggtgcga
    23881 caattgagaa agcaaggcat tccatgctac tttacgatgg atgctggccc taatgtcaaa
    23941 gttttagtgg aaaacagcca tttatctgag gtacaagaaa catttaccaa attatttagc
    24001 aaagaacaag taattactgc acatgctggt ccaggaattg cgattattga ataaagggag
    24061 atttttatga ttgaagtatc tgcaccaggc aaactctata ttgccggaga atatgcagtt
    24121 gttgaaacag gccatccagc agttatcgct gcagtcgatc aattcgtgac agtcactgta
    24181 gaatctgcac gaaaagtcgg aagtatccaa tctgcacaat atagtgggat gcctgtacgt
    24241 tggacaagac gcaacggaga attagtttta gatattcggg aaaatccttt tcattatatc
    24301 cttgctgcta ttcgcttgac tgaaaagtat gcacaagaaa aaaacattct tttatccttt
    24361 tatgatctga aagtaacaag tgagttagac agttcaaatg gccggaaata tggattgggt
    24421 tctagcggtg ccgtgaccgt tgcaacggtc aaagccttga atgtttttta cgcgttgaat
    24481 ttatctcagt tggagatttt caagattgca gcactagcca atttagcagt tcaagataat
    24541 ggttcctgcg gcgacatcgc tgctagctgt tatggtggct ggatcgcttt ctcaaccttc
    24601 gaccatcctt ggctccaaga acaagaaact cagcattcta tcagtgagtt acttgccctg
    24661 gattggccag gtctatccat tgagccattg attgctcctg aagatttacg tttattgatt
    24721 ggttggacgg gtagccctgc ctctacttct gatttggtcg atcaagttca ccgttcgaga
    24781 gaagataaaa tggtggctta tcagcttttc ttaaaaaata gtacagaatg tgtcaacgaa
    24841 atgatcaaag ggtttaaaga aaataatgta acgttgatcc aacagatgat tcgaaaaaac
    24901 cgacaattac tgcatgattt atctgcaatc actggggtcg tcatcgaaac gcctgctttg
    24961 aacaaattgt gtaatttagc tgaacagtat gaaggagccg caaaatcttc tggtgcaggt
    25021 gggggcgatt gcggaatcgt aattgttgac cagaaatctg gcattcttcc tttaatgagt
    25081 gcatgggaaa aagcagaaat cactccactg ccgttacatg tctatagcga tcaaagaaag
    25141 gaaaaccgat gaatcgaaaa gacgaacatg tttcactagc taaagccttt catgataaac
    25201 aaaaaaacga atttgacttc gtacggatca tccataaccc tttgccgcaa atagcagttt
    25261 ctgatgtcga tctaagtaca caggcagttg ggtttacact aagcagccca ttttatatca
    25321 atgcgatgac tggcggaagc gaaaaaacaa aaaaaatcaa ccaagatctt gcaatcgtcg
    25381 caagagaagc agatttgatg attgccactg gttcagtcag cgctgccttg aaagtccctt
    25441 ctttagccga tacttatacg attatgcgac aagaatatcc tcacgggaaa atcattgcca
    25501 acatcggtgc agggacttct gtcgaaagag ccaaagaagc tatccgattg tttcacgcag
    25561 atgccttaca gattcatttg aatgcacctc aagagttggt tatgcctgag ggagaccgtg
    25621 attttaccaa ttggaaagta cttattcaag aaactcaaac agccattgac gtccccctca
    25681 tcgtaaaaga agtcggtttt ggcatgacaa gagaaacgct caatgatcta gctgctttag
    25741 gagttcatac agttgatatc agcggtcgaa gcggaacaag cttcacccag atcgaaaacg
    25801 cccgtcgctc caagcgggaa ctgagttatt tagctgactg ggggcaatcg acagtctctt
    25861 cattgcttga agcaaatgaa gcagacactt ctatggaaat cctagcctcc ggaggtatcc
    25921 gcaatgccta tgacatattt aaagcccttt gtctcggcgc aaatgccgtg ggtacttcag
    25981 gaaccgtact gacgcatctg atgaatcacg gcgtagaaga aactattata ctgatgaagc
    26041 aatggcaaga agagcttcgt ctgctttata ctatggtcgg cgcaacgaat accgcggccc
    26101 tacaccaaca atctctgatc ttttccggac cagtgaagga ttggtgcgaa gcaagaggga
    26161 tcgacttagt gaagtatgga agacgcacag aaaagagcgt gggacaaaag taaagaacac
    26221 ttttgtctca cgctctaaac acgtacaaac ggcggaagta aaagcaactc cttcggaaat
    26281 aagctgaaat tcacaaaaat ttgaaaagca attttcgtga attccttctt atttcttgga
    26341 gttaaatgct tctgtcccag cctctttttt cgaataaatg atgtttaatc agctgtcctt
    26401 cggtattcgt tcaatccgct gatttcacaa cctctccact aaacagtgta ctaaagctct
    26461 tttaaggttg ttttccgata taggcaagta tgcctccgtc cacatacaag acatggccgt
    26521 taacgaaatc agatgcgcta gaagccaaga agacagatgg tccagccaaa tcgattggct
    26581 cgccccagcg tgctgctggc gtacgtccaa cgataaattg atcgaatgga tgacgttcac
    26641 cgttttcttg tgtttcgcgt aatggagcag tttgcggtgt agcaatataa cctggtccaa
    26701 ttccattaca ttggatattg tattgaccgt attcagaagc gatgtttttc gtcagcattt
    26761 ttaatccgcc ttttgccgca gcataagcac tgaccgtttc gcgtcctaat tcactcatca
    26821 tggagcagat attgatgatt ttaccgccgc ctttttcgat catatccgga ataactgctt
    26881 ttgaaacgat gaatggggca tttaaatcca cgtcgattac ttggcggaag tctgccgcat
    26941 ccatttcgat catcggtgtc cgtttgatga tccctgcatt gttgactaga atatcaactg
    27001 aacctacatc ttctttgatc tgttgaacca tcgcttgaac cgcttcttca tcggtcacat
    27061 cgcagacata accttttgca tcgatgcctg cttctttata atgcgctata ccttcatcaa
    27121 ctgattcttg attcaagtta ttaaatacga tttttgctcc tgctgtagct aaagagcgtg
    27181 caatctcaaa tccaatacca tagacagcac cggtgactaa tgctactttt ccatctaaac
    27241 gaaacatatc catactaaat tccatcattt tctctccctt atgcttattt taattgatcc
    27301 atttgcacca tatccatgtc tgtataggtg atattttctc cgcacattgc ccagatgaat
    27361 gagtaattgc ttgtacctac acctgaatgg atcgaccagc ttggcgaaat aatggcttgt
    27421 tcattgtcta caactaaatg ttttgtttca tctggtttgc ccatcatgtg gaacacacgt
    27481 gtatcttcat tggcataatc aaagtagaca taagcttcca tacgacgttc atgtgtatga
    27541 caaggcatcg tattccaagc gcttcctggt tctaaaatcg tataacccat ttgtaattga
    27601 cagctttcgc atacatttgg atggatgtat tggtagattt tacgttcatt cagtgtcagg
    27661 ccttcaccag tctccattgg ttttacttgg tcgatactga tttttacatt tggatattta
    27721 tgatgagctg gaacagaact aatatagaat ttagctgggt tttcagcatc ttgagaagaa
    27781 aatacaacat gtcgtgtttc ttttccgata tagtatccgt cttgtttttt catttcttct
    27841 ttttgcccat cgatctcgat gaatcctggt ccaccgatat tgatcacacc tagttcacga
    27901 cgctctaaga agtaatcaac acctagttct ttgtctaatg tgatttctaa ttctttttca
    27961 gtaggtgtca ctccaccaaa gatcattcga tcgttgtgcg tataagtcaa actgattttc
    28021 cctggttcaa ataccttttc tactaaaaat tcttttctca attgttccgt tgaataatga
    28081 cggatatctt ctggactgtg tgtataacgt gtttccattt cttgcatttt cttctactcc
    28141 ttctatcgaa tgatttttcc agagccgcat gctaaaaatt gtgcgacttc ttcttgacta
    28201 aattggttgc agtccccatg gatcgtatgc ttcaaggcag aagctgccgt agcaaaatca
    28261 atgatttctt gcggagacat attggataaa atcccatgga gcacgccacc tgcaaaagca
    28321 tctccgccac ctactcgatc cacgattggg gtgattctat ggacttggga ttccacatat
    28381 tcatggttca tccacaatgt ccccttcaat tcattttcac ttgctgaatg aacggtacgc
    28441 ttggtcgaat aaaacacctg aatattcggg aacttttcct gcattttttg ataataataa
    28501 attagaggtt gttcttctgc ttctggcgct ttctcgatac ctaacaaata aagagcatcc
    28561 atttttccag ctgagcagat atctactaaa ggtaaaagct gatggattac ttcgctggct
    28621 tcttgctgtg tccataattt tccacgatag ttgatatcga agctgatcag gcagccagct
    28681 ttttttgcag cttctatcac ctgtttcgtc attttttgcc attttttaga caaagctggg
    28741 gtgattcctg aaacatgaaa tatatctatt ccttggaata actcctctag cgaccattcc
    28801 aaatgatcca tttcagcaaa acttgatccg gcacgatcat agatcactga ggcggcgcgt
    28861 tcgccactac cagtttccat ataataagta cctagtcttg atccaccaga taatacctgt
    28921 ttcgtatcta ctccataaca ttgcaagtgc tttttgactg cttttcccaa tgcgttatca
    28981 ggtacttttg tagcaaaaat cgcttcatgt ccgtaattag ctaaagaaat tgctacgttt
    29041 gcttcccctc cgccataatg ggcagcgaat cgttcacttg aatcgatacg ggttccctgc
    29101 tctgtggaca atcgcaacat gatttctccg agtgtcacta cttttcccat aagcagctac
    29161 ctcttgattt ctttcatttt tgctgcatac tgttgcgcga cttccgttac tttgtcaaaa
    29221 tctcctgtag ctgcgggtgc taaaaggttc ccacctactc cgactgttac gacaccagca
    29281 tcgaaccact ctttcatatt gtccagactc acaccgccag ttggcatgat attgacttga
    29341 ggcatcggcg ccttgaaggc agaaatgata cttggtccat aagcgcttcc aggaaatagt
    29401 ttgacgatat ctaccccgct tttcagcgct gttttgatct cggtaatagt catacatcct
    29461 ggtaagtaag gaatttgata cagattgcac atctctgccg tttcttgatc aaaggaaggg
    29521 ctaacaatat actctgcgcc agctaagata gccaagcgag ctgtgacagc gtctaatact
    29581 gtgcctgcgc caatcactat ttctggttgg tctttgtaga atgacaaaag ttctttgatt
    29641 acttgatctg cttgagggac tgtaaaagtc aactcaatac cagttagacc acctttgatg
    29701 attgcgtggc aagcattcaa agcttcttct ttgctttttc cgcgtaccac cgcaatcact
    29761 ccggcgtttt tcaatcgaga taaaatatca atttttttca tcttctccac ctatttccta
    29821 tttagaaatt ttattttata acttggaaat ctatacccat attatacggc agaaaagaaa
    29881 aattgcaaac gcttttttca ttatttttga attctttgct cgatttcgct ttttgtttgc
    29941 ttgagggcac tgattatttg ttcttttgtc tcatctgtta aacgatattt cggcatactt
    30001 acactaaatg caccaatgat ttgttcgttt ttcacgattg aggctcctat acaaaaaata
    30061 tcacgttcga tttcttcatc gtcaaatgcg atgtgggttt cttttaccgt tttgatttcc
    30121 tttttcaacc gtaatgcatt ggtgatcgta tattctgtgt aagggatcaa cggatgcttt
    30181 tcaagataat cagtgtactg ctcttcactg aattctgcta atacggcttt gcccatcgca
    30241 gaattgtata atggtcgggt gattccgatt tttgaagaca tgcggatcgt ttgatgtttt
    30301 ggatctagtt tgttcacgta caagatctca ttgttgtcca atacccctaa gtggatagtc
    30361 tcatcgatct tttgctgcag ctgttctaaa aacggtaaag tcaactctac caaatcgatt
    30421 tgttcaatgt ttttattcgc gtagcgaata agctttgcac ctaaacgata gtttttctct
    30481 gaatttttat ttacgtagcc gatcatcaac aaagtatcta gtattttcaa gactgtagaa
    30541 gaggtcattc ccacaccctt tgcgatcgtc tgcaaagaga catcttggtt ttccgctaaa
    30601 tagtctagta ttttagaggc tttgatcaat acagttccat aaggcttttt at
//
LOCUS       DAHHZJ010000017        29260 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.018, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000017 DAHHZJ010000000
VERSION     DAHHZJ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.018"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            4..636
                     /locus_tag="NBL02_000627"
     CDS             4..636
                     /locus_tag="NBL02_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571335.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(770..895)
                     /locus_tag="NBL02_000628"
     CDS             complement(770..895)
                     /locus_tag="NBL02_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD2571336.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(1029..1922)
                     /locus_tag="NBL02_000629"
     CDS             complement(1029..1922)
                     /locus_tag="NBL02_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HCD2571337.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(2104..3030)
                     /locus_tag="NBL02_000630"
     CDS             complement(2104..3030)
                     /locus_tag="NBL02_000630"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HCD2571338.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(3115..3876)
                     /gene="pflA"
                     /locus_tag="NBL02_000631"
     CDS             complement(3115..3876)
                     /gene="pflA"
                     /locus_tag="NBL02_000631"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HCD2571339.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(4120..6351)
                     /gene="pflB"
                     /locus_tag="NBL02_000632"
     CDS             complement(4120..6351)
                     /gene="pflB"
                     /locus_tag="NBL02_000632"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCD2571340.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(6628..9078)
                     /gene="parC"
                     /locus_tag="NBL02_000633"
     CDS             complement(6628..9078)
                     /gene="parC"
                     /locus_tag="NBL02_000633"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCD2571341.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDRSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(9090..11135)
                     /gene="parE"
                     /locus_tag="NBL02_000634"
     CDS             complement(9090..11135)
                     /gene="parE"
                     /locus_tag="NBL02_000634"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCD2571342.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(11301..11735)
                     /locus_tag="NBL02_000635"
     CDS             complement(11301..11735)
                     /locus_tag="NBL02_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCD2571343.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11862..12500
                     /gene="plsY"
                     /locus_tag="NBL02_000636"
     CDS             11862..12500
                     /gene="plsY"
                     /locus_tag="NBL02_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCD2571344.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(12632..13507)
                     /locus_tag="NBL02_000637"
     CDS             complement(12632..13507)
                     /locus_tag="NBL02_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HCD2571345.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(13589..14380)
                     /gene="codY"
                     /locus_tag="NBL02_000638"
     CDS             complement(13589..14380)
                     /gene="codY"
                     /locus_tag="NBL02_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HCD2571346.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(14398..15798)
                     /gene="hslU"
                     /locus_tag="NBL02_000639"
     CDS             complement(14398..15798)
                     /gene="hslU"
                     /locus_tag="NBL02_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HCD2571347.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(15812..16360)
                     /gene="hslV"
                     /locus_tag="NBL02_000640"
     CDS             complement(15812..16360)
                     /gene="hslV"
                     /locus_tag="NBL02_000640"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HCD2571348.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(16397..17302)
                     /gene="xerC"
                     /locus_tag="NBL02_000641"
     CDS             complement(16397..17302)
                     /gene="xerC"
                     /locus_tag="NBL02_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCD2571349.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(17500..18876)
                     /gene="trmFO"
                     /locus_tag="NBL02_000642"
     CDS             complement(17500..18876)
                     /gene="trmFO"
                     /locus_tag="NBL02_000642"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HCD2571350.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(18845..20923)
                     /gene="topA"
                     /locus_tag="NBL02_000643"
     CDS             complement(18845..20923)
                     /gene="topA"
                     /locus_tag="NBL02_000643"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCD2571351.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(21045..21902)
                     /gene="dprA"
                     /locus_tag="NBL02_000644"
     CDS             complement(21045..21902)
                     /gene="dprA"
                     /locus_tag="NBL02_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCD2571352.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(21958..22725)
                     /locus_tag="NBL02_000645"
     CDS             complement(21958..22725)
                     /locus_tag="NBL02_000645"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCD2571353.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(22718..23578)
                     /gene="ylqF"
                     /locus_tag="NBL02_000646"
     CDS             complement(22718..23578)
                     /gene="ylqF"
                     /locus_tag="NBL02_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HCD2571354.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            23838..24236
                     /locus_tag="NBL02_000647"
     CDS             23838..24236
                     /locus_tag="NBL02_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCD2571355.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            24515..25015
                     /locus_tag="NBL02_000648"
     CDS             24515..25015
                     /locus_tag="NBL02_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HCD2571356.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(25098..25649)
                     /gene="lepB"
                     /locus_tag="NBL02_000649"
     CDS             complement(25098..25649)
                     /gene="lepB"
                     /locus_tag="NBL02_000649"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCD2571357.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(25801..26028)
                     /locus_tag="NBL02_000650"
     CDS             complement(25801..26028)
                     /locus_tag="NBL02_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HCD2571358.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(26025..26543)
                     /gene="msrA"
                     /locus_tag="NBL02_000651"
     CDS             complement(26025..26543)
                     /gene="msrA"
                     /locus_tag="NBL02_000651"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCD2571359.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQDYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(26563..27276)
                     /locus_tag="NBL02_000652"
     CDS             complement(26563..27276)
                     /locus_tag="NBL02_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HCD2571360.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(27279..28175)
                     /locus_tag="NBL02_000653"
     CDS             complement(27279..28175)
                     /locus_tag="NBL02_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HCD2571361.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(28333..29175)
                     /locus_tag="NBL02_000654"
     CDS             complement(28333..29175)
                     /locus_tag="NBL02_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCD2571362.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
BASE COUNT         7718 a         6145 c         5040 g        10357 t
ORIGIN      
        1 attatggaaa ttcaacgtag aaccactaat tttttacatt taggctcttt ttttgcaact
       61 ttgacgatct tattttttct atgttattca taccttaaga cctcacatgc gccttctagt
      121 gcattaggct ggttgctttt ggatcattct gatatcacag aagccgaaga aaatccgttt
      181 tttttgattt tgtcttctct ttgcggctta ctgttttctg tttatttcgg tgcctatttt
      241 tttcatcgac atcctggtac gttaaacgaa gcttcaagcg tattgattgc atttaaaacg
      301 ttttcttttt tagcaattct tttttattcg tttaatcaca atacgctttt cttctttatc
      361 atgaatggtt tagtcgtgat tttatccatt tttacttatt ttattttttc actcattttt
      421 agccaattga aatgtccgct cttctttcga ttagttcctg tcacctgttt tatttacagc
      481 ttgttttcct tttttgtcct tgctttaatg ccactgacca acccttcgac gatccaagtc
      541 gcaggaaatc tgttggatat gctcttcatc gtcagtctag gtgtatttat gtggcaaaga
      601 aaaaacaaac aaaataaaaa gcattttgaa gcctgactat tacgacttca taaatgcttt
      661 tttagtttta aacacttcaa tctaaaagaa taacccaaga ttggataaat tattaatgac
      721 tcgccattcg tttcttagtg attctggatt atcaaatgga cttcctaatc taatcagcta
      781 gttcttccca taaaatgcca tacttgactg aatcataata ttgattattt aaaaatctaa
      841 cttttcttat cactacttcc tttgtcattc cacatttctc accatttttt tgcattcctt
      901 tgtttccaga ccatgtagtg aatctaatag gtggtagata ggcaaaatac tcaaataatt
      961 cttttaacca taaaggaaga gaaaggctcc tcctgcaaga gtcagtataa tttttgctta
     1021 ctgctctctt aggttttact cactaaaact gttccatatt ctttgatata agaaaatgca
     1081 tgcggtataa atttcttgct cagctcagaa aagatttgtc gtttaagaaa ggccatttcc
     1141 tccattgtca atgaaggatc caatgttggt aattcaatct caaaattgtt tttttcgaca
     1201 ataggtaatt ctaatgtgaa tgattcttct tcctttatag tattcgtcac aagtagagac
     1261 tgatggaaaa tactaggccg gcgattttta tatttattct tacgtatcct gtcaacgata
     1321 acttctattt gatcaaaaaa gtacgattca tttacgtctc caataagttc ggctaattct
     1381 ttgaatgcct tcgttacagg aagctgattc ttctgtacaa aaaagtaatt agccaaagta
     1441 agcgggaagt gttctataga ggaaagtgtg ataaaacggt acttctcatt tcctgcagtt
     1501 gtcctcagca attctgtctc tcggctgttt cttactccat aaaaatacgc ttctttccgt
     1561 tcatcttcga aacaatacgc ccaaataact tctcccatcg ctaatttttg ttcatcaaca
     1621 gagagtctct tcaattgttc agctatggtt tcagcagccg aagtggcttg ttgcaagtaa
     1681 tctttaggat caaatattgg aaagttcaaa tgatatcttc gttcttccct cttgattaat
     1741 cctgcatcga tcaataaatc aagaaaatgg gaaaatttat tttgcgaaaa ttcttttttc
     1801 aattctctta gaatcgtttc ttgttctcga tgttcatata gataatcggt caatgaagaa
     1861 aaaagcggat catcggtcag tttttgataa aagttttcct tattttttga ataatagttc
     1921 atatatgttc tcttttctat ccgttttatt ttacttaaaa ggcttttacg taaaaaggag
     1981 gaagaacaag agtcataatt gactgttatt ctcctccatt tcttcggcct ttagtatgtt
     2041 ctatgtttct ttttatatca atcgcttatc cattcatttt gaatgcttca tactaccatt
     2101 tgattagcta aatacagccg tcaattgagg tacgacttgt tttttacgtg aaacgacacc
     2161 tttaagcaaa gcacggttgt tggtcaaagt tgtttggaaa gcttcttcta ctttttcttt
     2221 tcctttacca gcaacaagta attctgaatc actgtctaag atgtttgtga caactaatac
     2281 gaacagatcg tagccatttg ctttcatttc atcttccatt gctgcttcta gctctgcttg
     2341 tcgatccatc acttcattaa ggtccactgt attgacttga gcgatacgaa cgttggaact
     2401 gcccattggg aaactttttg catccaaatc aagaagaacc gcagctgatt tatcacttag
     2461 atttgttcct gctttcaaca tttcaagtcc ataagtgttt agatcgactt cagcgattgc
     2521 tgcaagttct tttgcagctg cttcatcttc aggcgtacat gttggtgatt tgaacaacaa
     2581 tgtatctgaa atgatagctg agagcatgat tcctgcgatt tctttaggaa ttgttacttg
     2641 gttttcttta tatagtttta aaataatcgt acttgtacat ccaactggtt ctgcacgata
     2701 gtacaatgga ttagctgttt caaagtttgc aatacgatgg tggtctacta cagctaaaat
     2761 atttacttcg ctgatatctg aaatactttg ttggaattca ttatgatcca caagcatgac
     2821 atctgcattt tctgctttag ctgactctac tactcgtggt gcagatagct gaaagtggtc
     2881 taatgcgtat tgtgtttctt cattaggtgt acctagagca actgcttctg tttctttacc
     2941 taattcattt tgcaagtgtg aaaacgcaat ggctgccccg attgcatctg tatctggatt
     3001 ttggtgacca aaaactaaaa tttttgacat gtcattcgct cctttgtgat ttctcctatt
     3061 taataatagc aaaaaaacgg aaagaagcaa tacttatctt ctttccgatg cgacctatcg
     3121 cgttaaatat tttgtatact cttctgtatg aagcaatcgt ttagcatttt cgacacgttc
     3181 ttttgtaggt ggttcgatcc cttctaatgg atatttcaaa ccaagtgttt cccacttgta
     3241 ttttcccatc gtatgataag gaagaatctc tactctgtct acatttgaca aggtttgaat
     3301 aaagttgtct agtcggatca aatattcatc ataatcactt cgctcaggaa ctagtacatg
     3361 gcgaatccaa accggtttgt tgatttttga caaatattga gccatttcta aaatattttc
     3421 attgtcccaa tgcgttagtt ttttatgctc ttcattatcg atgtgtttga tgtcaaataa
     3481 cagtaaatct gtatacttca tcaattcttg aaaacgtgaa aagaaaggtt cttcatacgt
     3541 aaaaggtttt ccgcatgtat ccaatgtagt atgcatcttc cgctcttttg cttttttaaa
     3601 tagttcgatc aaaaaatcga tctgcaatag aggttctcca ccactaacag taataccacc
     3661 tttatctccc cagtaaggtc tgaagcgttc tgctttatct aaaagttcat ctgctgtgta
     3721 ttctttccct ccgccaatat tccaagtatc tggattgtga caaaattggc aacgcatgcg
     3781 gcatccttgc ataaacacga caaagcgcag tccaggtccg tctactgaac cgaaagtttc
     3841 gatagaatga acatagccga ttgttttttc ttccatttaa gttcacatct tttctttgat
     3901 ttcattttaa gtacaagtta gctgtcgaag cgaagctttc tgaacaaaat ttttcgcaat
     3961 tgccgcttcg ttatgtgtaa aatttacagg gacggaacat acaagtcctg cccctgtaaa
     4021 tcgtttcttt caaataagca tactgattat tcgattttga tgcgatggct attcaacgtc
     4081 ctttcgacgc taagtcgaaa aacttttttc acgaactttt tacattctgt cgtgtgaagt
     4141 tctcatcaag acatctaatt gttgttctct tgtcaagtca cggaatttaa ctgcataacc
     4201 agatacccgg atcgtcaagt tcggatattt cattggattt tccattgcat cacgcaataa
     4261 atcattactg aatacattga cattcaaatg ataagctcct tttgagaagt atccatctaa
     4321 gacattacgc aagttttcga ttcttgtatc ttgttctttt ccaagacctg aaggattgat
     4381 tgtttgggta tttgaaattc catccaatgc acagccgtaa ttcattttag ctgttgagtt
     4441 caggctagct aataacccac tttgttccgc attgtaactt ggatttgctc ctggcgcaag
     4501 tggttttcca gcttcacggc catcaggagt atttcctgta tttttaccgt aaacaacatt
     4561 tgaagtaatc gtcaataatg aagttgttgg ttttgagttg cggtaagttt tatgacgttt
     4621 gatttgtccc atgaagtatt ccaagatcca tgaaactaat tcatctgcac ggtcatcatc
     4681 atttccgtat ttagggaagt cgccatccac ttcaaaatca actgctattc ctttttcatt
     4741 acggattgct tttacttttg catatttgat cgctgacaag ctgtctgccg catgagagat
     4801 cccagcaata cctgtagcaa atgtgcgttg caagtcagaa tccatcaaag ccagctgagc
     4861 tgcttcataa gaatatttat catgcatata atgaatgatg ttcaacgtat tgacatatag
     4921 ttctgctaac caatccatca tattcatgaa gttatgtttc acttcttcaa agtctagtat
     4981 ttctgatgtg attggacgat attctggtcc aacttgggcg ccagtcactt catcgactcc
     5041 gccattgatg gcatataata gtgttttagc caagttagca cgagcgccaa agaattgcat
     5101 gtcttttccg acaactgttc ctgatacaca gcaagcaatc gcacaatcat cagaacccca
     5161 ttgttcacga agctgattat cattttcgaa ttgaatcgaa gaactttcga tagcgattct
     5221 gctagcatat gttctgaatc cttctggaag gtgttcagaa tacaaaacag tcaagtttgg
     5281 ttctggtgaa ggtcccatat ttgttaatgt atgaagcaga cggaaatcat tctttgtaac
     5341 taatgaacga ccatctaatc ccatacctgc aatggataaa gtagcccaga tagggaatcc
     5401 tgagaataat tggttatatt caggtgtacg cgcgaatttg accatacgta atttcattac
     5461 taagtggtcg atcaattctt gtgcttcttt ttctgtgatt actccattat ccaaatcacg
     5521 ttggatatag atatcaagga atgctgagat acgtccgatt gacatcgcag caccattttg
     5581 tgatttgatt gctgctaaat atccgaagta tagccattgg acagcttctt ttgcatttgc
     5641 agctggttta gaaatatcat agccatattt cgcagccatt tctttcattt gcgctaatgc
     5701 acgatattgt tcagtgattt cttcacgtaa acgaatcact tcttcactca ttgtatgata
     5761 accagtcata tcatgatctt ttttcttttg ttccatcaaa tagtcgattc catacaatgc
     5821 cacacgacga tagtcaccga tgatacgtcc gcgaccgtaa gcgtctggaa gccctgtaat
     5881 gattttgttt ttacgagcag cacgcatttc agcagtataa gcgtcaaata caccttgatt
     5941 atgtgttttg cgccagtcag tgaaaatatg tgtcaattca tcgtttggtg tatagccatt
     6001 gctttttagc gcattgttag ccatattgat tccgccgaac ggcatgaatg cttgtttcaa
     6061 aggtttgtct gtctgtagac cgacgatttt ttccaaatct ttatccaaat agcctgcatc
     6121 gtgtgaagta atagttgata cgatttctgt gtccatatca tagacaccgt tacgctcgaa
     6181 ttggatatca tttaactttt gcaattcatc ccataatttt tctgttgctt ctgttgggcc
     6241 ttccaaaaag ctgtcatcac cgcgatattc agtgtagtta ttttgaataa agtctctagt
     6301 gtctacgcct tctttccatt tggtaccttt aaatccattc cattgctcca ttgtatggac
     6361 ctcctaaatt tctgttttat gtaacagttt ttacgtgatt agtataacac agcaccctat
     6421 ctttgcaagg gttttcttgt tttttattta acaattttta tgttttattt acatatattt
     6481 aaatcaacgt tgacatacca acatttaagc attgtgaaca ctttcatctt tttttgcaaa
     6541 cgtataagga tttattaaaa atgaatgaaa aagagacgcc acctgtatta atacagatgg
     6601 cgtctcttca ttaaaatact acaacagtta cgactcgatg aaaatctcag gagcatcgat
     6661 aacttcgatg acttctccac cttttttctc atccatcgca aatgatccat tcgatgttcg
     6721 atcacctata gggaaatcag ttgggtcaat cgttaactgc tgcccttttt gattcatcaa
     6781 ggttatttct tttgatcttc cttccgacat atagataata cggtgcggat tttttttcag
     6841 ctcacgcatg accgtcactc cccgcttcgc acgtgaagtt tggctgattt cttgagcaag
     6901 catgcgttta acagctcctc gctgagtaac gattacgatc ggcgtatcac cttctgctaa
     6961 cacaagcagc ccgttgataa tcctatctcc atctttcaaa ttcatagact tcactccagc
     7021 tgcctttgct ccaacaacgg gaacatctgt taaaggatag cgcaaaccca ttccttgatg
     7081 cgttactaag aaaacatcta agcctggctg atcttctttc agatacacag caatcagttc
     7141 atcttcttcc gcctttagct tcatacagct gagcggacgg ctcttatagg tgcgccatgg
     7201 ttcgaactct gtcattcgtg tttgcttgat caatccattc ttgctgatga atacaaatgt
     7261 tttctctgct tgaatttttt gatatgggaa aacagctaaa atcgattcgt ctacagacag
     7321 attggagatt gtttgagaaa tgtgctcacc agcatctttc catttcaaat cgggtagttc
     7381 atggacaggg cggtagatca catttgcttt atttgtgatc agaagaacat ggtccaatgt
     7441 actcaattca cctgagtata gcaagtagtc gccttctcgc atcccgattt cttctggttt
     7501 ggaagcacta taagaacgaa ggcttgttcg cttcacatag ccttctcgcg tcacactgac
     7561 aattacgtct tcttgtgcca ctaagacttg cgtatcaatt ttgatttctt cgatttcgtc
     7621 ttcgattgtc gttagacgag cggaagaata attttttttc acttctcgca attctttttt
     7681 cataacagag aaaagctctt tgtcatttga aaggatttta ttcaactctg tgatttgcgc
     7741 aatcaagctt tcggattcct tttgaagctc tgtgatatcc gtgttcgtca aacggtataa
     7801 ttgcaatgtg acaattgctt ctgcttgttc ttctgtaaat tgaaagacgt cgactaaatt
     7861 ctttttggcg tcttttttgt ctttactttc acgaatcgtt gcgattactt catccagaat
     7921 cgataaagct ttcatcaatc cttctacgat atgctgtctt ttttgagctt tggctaaatc
     7981 gaattgacta cgttttgtta tgacttgctt tcgatgctcg atataacttc ttaaaatgtc
     8041 tttcagtcca acttgatgcg gagtcatatg gtcgatagcg accatattga aattgtaatt
     8101 gatctgtagc tcagtgtttt tgaacaaata attcaaaatt ccttgagcat tggcgtcttt
     8161 tttcaattcg acgacaattt gcagacctgt tctatcactt tcatctcgta cttcagctat
     8221 tccatcgatt ttcttattca aacggatctc atccattttt ttcaccaagg tcgctttatt
     8281 tacttcatat gggatttctg tgatcacgat ttgctgttta ttacccttca ttgcctctat
     8341 ctttgttttc gaacgaagaa taacttttcc tcgtcctgtt tcataagctt ttttgatttc
     8401 atcttttcct tgcaagatcc ctcctgttgg aaagtcaggt ccaggaatat attccatcaa
     8461 tttttcaagg ctcgcttgtg gatgatcgat catatagatt gtgccatcga tcacttcagc
     8521 taagttatga gtcgggattt ctgttgcata tccagcagat atacctgtcg atccattgac
     8581 gagaaggttt gggtatcgag ctggaaggac tgttggttct ttttctgtat catcaaaatt
     8641 ccacacgaga tcgactgttt ctttctcgat atctgctagc atctctccac ttaacttgga
     8701 taatctggct tcagtataac gcatcgcagc aggtggatca ccgtccatac tcccgttatt
     8761 cccgtgcatt tcgatcagta cttcccgcaa cttccagtct tggctcatgc ggaccatcgc
     8821 ttcataaata ctgcggtcac catgagggtg ataattcccc atgatatttc cgacagattt
     8881 tgctgattta cggaatcctt tgtcatacgt attgccatct ttgttcatgg caaataatat
     8941 ccggcgttgc actggcttca atccatcacg gatatcaggc agtgcacgtt cttggataat
     9001 atatttggaa taacgtccga agcggtcacc catcacttct tctaaagtta attcttgtac
     9061 ttcttgtcga ttttccatca ggtgacctcc tattctactt caaataactg attgttttca
     9121 tttttatctg ctcgttcttc atctaataaa tcattagaaa ctgaaggaga gatttcagta
     9181 tcctcttttt tatctaagat actgccatct tcttcgagcg taaattgcac gtgattttcg
     9241 atccattttc ttcgaggttc tactttgtca cccatcagtg tggttactcg acgttcagct
     9301 tgcgcagcat catcgatacg gacgcggatc aacgtacggg aagtcggatc catcgttgtt
     9361 tcccacaatt gttccgcatt catctctcca agtcctttat atcgctgcag catatatcct
     9421 ttgccgactt ttttaatcat tgctgccaat tcgtcatctg tccacgcata ttcaatgact
     9481 tgcttctttc cttgtccttt ggatacttta tataaaggcg gtaacgcgat atatactttc
     9541 ccagcttcaa tcagcggttt catataacga taaaagaaag taagcagtaa tacttgaata
     9601 tgggcgccgt ctgtatccgc atcggtcatg atgattactt tgtcgtaatt acagtcttcg
     9661 atagaaaact caggaccgac tcctgcacca atcgtataga tcatcgtatt gatttcttca
     9721 tttttcaaga tatcttgcat tttagctttt tcggtattga tgacttttcc tcgtaaaggt
     9781 agaattgctt ggaactttct gtctcttcct tgtttagcag atccgccagc agaatcccct
     9841 tcgactaaat acaattcatt tttcttaggg tttcgggatt gtgcgggtgt caattttcct
     9901 gaaagaagag attcgccttt tttacgcttc ttcccatttc ggctttcttc tctggcttta
     9961 cgtgctgctt ctctcgcttc tctggctttg attgctttac ggaccaacat ctggctcatt
    10021 tcgctatttt cctgcaaata aaagcccatt tgttcactga tgacgttatc tactacggta
    10081 cgagcaactg gcgtaccaag tttttctttt gtttgccctt caaattgcag aagattttca
    10141 ggtacacgga tagaaagtac agcagctaac ccttctctga aatcactacc ttccagattc
    10201 ttgtcccgtt ctttcagcaa accaactttt ctcgcatatt cattataaga tttcgtcatc
    10261 gccgctttca taccagcttc atgggttccc ccatcttttg tccgaacgtt attgacaaaa
    10321 gataaaacat tttctgaata accgtcattg tattgataag cgacctctac ttcgatacct
    10381 tctttttctc ctgaaaaata aacaactggt gtcaatgtat ctttttcttc attcagataa
    10441 tcaacaaatt ctttgattcc ttcttcgtaa tgaaaaattt ccttgactgg ttcttctcca
    10501 cgaagatcag aaagagagat tttaacgcct tttagaagaa aggcagattc tcttaaccgt
    10561 tcggctaaaa tttcatatga aaatttcgtt gtactgaata ttgtatcatc tggtaaaaaa
    10621 tggacagtcg tcccgtttgc tttttttgtt tttcctatct ttttcaaagt accgtcaggt
    10681 ttaccgccgt ttttaaattt ctgctgatac tctacgccat ctctaacgat cgttacaatt
    10741 agccatttcg ataaggcatt taccacactg gctcctacac cgtgcagtcc accagaagtt
    10801 ttgtaccctc cttgaccaaa ctttccgcca gcatgcagca cagtaaaaat cacttcaact
    10861 gttggtattc cggaagcatg cattcctaca ggcatcccac gtccgctatc tgcaacagtt
    10921 atactattgt cttcatggat ggtcacatca atctcactgc cataacctga taatgcttcg
    10981 tcaactgcat tatcgacgat ttcatagact aaatgatgca agcctcggct gtcagtggat
    11041 ccaatgtaca tccctggtct ttttctaact gcctctaatc cttccaatac ttggatggac
    11101 gagtcattgt attcgttgtt tacttttttt gccaaggcaa acgctccttc aataatactt
    11161 tgcggcaaaa ccgcaccacg attatcatac tagaaaaggt cgtgaaaaaa aagagggtag
    11221 acgagaaatc aattttaatt atctcctaac cgctttttcg acattcccct ctatcgatca
    11281 tctcttcttt gtggtcattt ttatttttgg ttttctttca acttaccaag ttcaatctta
    11341 atacaacgat ccattacgat atctgtacgt cctgcttttc ttagcatctc agctgcttct
    11401 ttgttttcaa tccctaattg tgcccagaac actttagcat cagttttcaa aaaatcacct
    11461 gctatttcag gtaaaaattc actccgtcta aatacatcta cgatatcgat tggatcatcg
    11521 atttctgtca aattcgcata gactttctcg cctagaattt cttcaccagc caataatgga
    11581 tttactggta tgattttata tccattgtct tgcaatacct gtgcaaccat gtaacttgtt
    11641 cgttctggtt tattgctcaa gcctacgacg gcaatatttt ttgcttcttc caaatagttg
    11701 aaaatcgttt cttgtgttgg attttttaca gacatttctg ccaccccttg cttttctttt
    11761 tattccatct attatacttg tatctttcat tttttcaatt gttcctgtta cctattcatg
    11821 ctaaaataaa ggatagtttt caacttgaaa gagggtaaaa aatgaaattg atcttattac
    11881 ttattttagc atatctttta ggttctattc cttccggtgt ctggattggc aaattgtttt
    11941 tcaaaaaaga tatccgacaa cacgggagcg gtaatactgg aacgaccaat acatttcgtg
    12001 tattaggcaa aacagccggg attgttgtcc tgcttatgga tattttaaaa ggtacattgg
    12061 ctacttgttt gccgatgatc ttccaccttt ctgggattaa ccctctttgg tttggggtat
    12121 gcgccattct tggccataca tttccgattt ttgctgggtt caaaggagga aaagctgttg
    12181 cgacaagtgc aggaatgctt ttaggattta atcctgtatt ttttgtttat tcttgtatca
    12241 tctttatcgt tagtttattt tgtacaagta tggtgagttt gagcagtatg atcagtgcag
    12301 tattgatcac tttatctacg atcgtccttc cttatgtagc tcctgtgatt ttggctaagc
    12361 ccaattggtt actgacgatc attgcctttt tagtttcttc ctttatcttt tatcggcata
    12421 aagacaatat caagcgtatc agaaaccata ctgaaagtcg aatcccgttt ggtttgaata
    12481 gcagcaagaa aaaatcctga tactttttca ttccaaagag ataaaacggg agtgtgacaa
    12541 atcgatgtca cactccctta ttacttacag gatagctttt ttacacacct tggtaaacgg
    12601 tgttccctgc taacttctcg gtcacaacct cttacttcac tgtgatggaa tattttgtct
    12661 tgaagatttc atgggcacct agttgattga tccctacttt ttccatcaag tttccactac
    12721 tgttcaccgt gtcagcgatt ccgcaccacg gttcgataca aacgaaaggc gcttcttttg
    12781 gataaggaga ccagatccct acataaggca tatctttata gcttaaagtt acgctatggg
    12841 aagatttttc acttcgaatg gcgtacgcat ttagacccct tgtttcaaaa accaaggcat
    12901 cttgctcaaa aagttcacga ttcaaagcca gattcgtatt tgtctgacct aacgtctttt
    12961 gatccatatc gatgaatggg cctttcaatg gtaggcgtaa tcttgatttc aatggagaaa
    13021 atgccagata gaaatcttca aatgacaatc cttcttcaag aggtacattg aaagcaggat
    13081 gtccaccgat agaaaagaac atttcttttt caccggtatt tgccacttga taatgaaccg
    13141 tgatgccgtc tcctcctaat tcatagctga tcactaattc aaaatcaaat ggatactttt
    13201 ctaatgtctc cggcgtactc cttaagatga aactagccat ttcttgtcca tgttcaatga
    13261 cttcaaaatc catatcccga gcaaatccgt gttgccccat ttgatatgtt ttcccttgat
    13321 aaacatactg atcatctttt aaacgtccaa caaaagggaa taaaaccggt gcatgtctgc
    13381 cccaatacgc tggatcacct tgccagatat actctaaacc atgttctttt gatttcagac
    13441 tgatcaattc cgcaccgtgt tcagcgattt cggcgatcag ctggtcattt tgaataataa
    13501 cagtcattgc cattcctcca acttcgactt actttttaaa atgggaaggc tggaacaaac
    13561 atttctatct gccccagtcc ccttacttct aataactgtg ttttgctaac tcgtctttaa
    13621 aacgggaatt caatactttt aaataagttc ccttcatgcc taacgaacga gattcaatga
    13681 ttccagctga ttccaatttt cgtaaagcat tgacgatcac agaacgagtg atgccgatac
    13741 tatcggcaat attagaagca gtcagacgac cttcatctcc atccaatgct tcaaagatag
    13801 cttgtacagc cttcaattcg ctataagata atgtgttgat tgccatttgg acagccgttg
    13861 cactccgtac atctgcttcg atgcttcgag acttctgata aaggatctgc ataccgacaa
    13921 ccgtagctcc atattctgct aagactagat cgtcatctgc aaacgattgt tccatacgag
    13981 ataagataat cgtacccaaa cgttcgccag ctccaaagat cggaacgaca gtcgtcagac
    14041 caaatggata tttctctctt gactctaccg gaaatgcagt aatatcacta gtaattggga
    14101 tattagcttc agttttcatc aactgtgcca ctgtatcagt ataagattca ggaaattgtt
    14161 tctcaacaaa cattgttttg attcgttcat tgttcacatc gtgacgttca ttataaccta
    14221 gtaatactcc gtcacttcct atgatatagg tgttgctgtc aagaatacca cctagtgtga
    14281 cagccatttt atcgtaaggc aaatctgctt gcatgtcaaa agtattcttt tgctgcaaaa
    14341 gtgtgtttat cttgcgggtt ttcgttagca agtctgtcat tgtcgtcctc cgatttttta
    14401 taaaatatag cgacttaaat cttcatttgc cgcaattgag ccaagtttgt catccacata
    14461 tgcttctgtg atcgttattt ctcccatttg catatctggt gcttcaaata atagatcttc
    14521 tagtagtttt tccaaaatgg tatgcagtct gcgtgcgccg atattatctg tgtcacggtt
    14581 gacatcgaaa gcgatttgag cgatccgttc aattgcttct tttgtaaacg taactgacac
    14641 attttctgta ccgatcaacg cgacgtattg ttttactaaa gcattatttg gttcggtcag
    14701 aatcttcaca aaatcttcag ctgtcaaatc atctaattct acccgtatcg ggaagcgtcc
    14761 ttgaagttct ggaattagat cacttggttt agacaaatgg aaagctcctg aagcaatgaa
    14821 taggacgtga tctgtttgga tgatcccgta ttttgtgttt acttgtgaac cttcgacaat
    14881 tggcaatatg tctctttgga cgccttctct ggatacttct ccagattgct gtgatttaga
    14941 agtgatctta tcgatttcat cgataaaaat aattccactt tcttccgcta atttgattgc
    15001 ctcactatgg atatcagcat ccttgactaa ttttgctgat tcttccttga tcaataactc
    15061 taaagcttct ttgactgtta ccgtccgttc cacttttttc ttcggtgtca aagcacctag
    15121 agtttcattt aggtcgatcc ccatttgctc caatccattg ttcatcattg gtgcaggttt
    15181 cttttggtct tcaacttcaa tggttacttc acgactatcc agtaaaccct tttgtagttg
    15241 ttccaagact gtacggcgat tgattcgaat atcttctgtc acttcttctt gcggttcagc
    15301 ggtattttgc tgactagcat taaaaagatt catcatttgt tcaaattgat tagtggattg
    15361 tttctgttct tttttgatac caggtaccaa gatttttacc aaacgttttt ctgcattttt
    15421 tttagcactt gtgtatacac ggctgtactg ctctttttct acgatttgga tcgcattttc
    15481 tactaaatcc cgaaccattg attctacatc tcttccgaca taaccgactt cagtaaattt
    15541 tgttgcttcg actttgacaa atggtgcatt gacgattttt gccaaacgtc gtgcgatttc
    15601 tgttttaccg acccctgtag gtccgatcat cagcatattt tttggtgtaa tatcttgttg
    15661 cattttttca tctaactgca aacggcgata gcggttacgc aaagcaacgg caacagattt
    15721 ttttgctgcg tgctgtccta cgatatattc atctaattca cttacgattt gtcttggtgt
    15781 ttgttttaat tcatacatat ctactcacct cttacagttc ttctacgata atattatgat
    15841 tagtaaagat acagatatct gcggcaatat ttaacgcatt ttctgcaatt tctttcgcac
    15901 tcatgtcgtt ttgagcatag tttttcattg ctcgggctgc agccaacgca aaattccctc
    15961 ctgatccgat agctaagata ccatcatctg gtgtgataac ttctcctgta cctgaaacaa
    16021 gaagcatttc tttatcattc attacgatca gcatcgcttc aagtttttgc atagattgct
    16081 gtgtccgcca ttcttgagct aattcgacag ctgcgcgttg tagattcccg ttatattcgt
    16141 ttagtttgcc ttcaaatttt tcttctaaag taaacgcatc tgcaacactt ccggcgaaac
    16201 ctacaacaac ttctccattg tagatacgtc gtactttctt tgcagtgcct ttcatgacaa
    16261 cagattcacc cattgttact tggccatctc cagccatagc aaatttcccg tctttttcga
    16321 cggcacagat agtggttgaa tgaaatggtg attcgaccat gtttgtttcc tcctaaattt
    16381 gttcttttcc ttatttttac gctcttggat ggaaagaacg atagtttttt tgtaaacttt
    16441 cttttgttac atgggcatag atctgggtag tcgataaatt ggcatgtcct agcaattctt
    16501 ggactgtccg caaatctgcc ccattattta ataaatgagt agcaaatgta tggcgcagca
    16561 tgtgaggatg gatatcacta tccaaactgc tttttttgat catttgattc aagacatatt
    16621 caattccagt aggcgtgatt ggttttccat gatgattcac aaaaacgata tcatgctctt
    16681 cgtgatattt tgtcatcaat acctggcggc cttccctgaa atattcttgc agagcatctt
    16741 gagcaaaaga acctagagga gcatatctct ctttgttccc tttaccatgg atcaacatga
    16801 cctctgaatc aaaatcaatg tctgataccg acaaactgct acactcactt aaacggatgc
    16861 ctgacccgta aaggacttcc agtaaagcgc ggttcctcaa atccaaagga ctatctcctg
    16921 ctacgctgtc aaaaagtgct tgcatttcat tttcataaaa aaaacgtggc agtctcaaat
    16981 ttttcttttt gagatgaacg taagaaaatg gattttcttt tatcacttct tctttgagaa
    17041 gatactgata aaacgaacgt aggctggcta tcttgcgact gatagaattt ctgctatagc
    17101 taggacgatg atcagctaat gcactcaaat agacacgaac cgtaaaatat tccgccttca
    17161 gcagatcagc gtctccagat tcctccaaaa aatgtttgaa atggagaatg tcttcttcat
    17221 atgcttcttt cgttttttct gaatatcctc tttcgacaat caaataacgc aaaaattttt
    17281 gttcccacgt ggcttctgtc attttctcac cttccttttg tcccagagaa taatttagca
    17341 cagcgtttat aaaaagacaa ataaattgtc agaatttaac caatatttct aagtttgttc
    17401 acaattggcg caattcctca cattttgttt gagaaattct taagaaaaac gcaagaaaaa
    17461 gagcgagact atccgtctca ccctcttcat ggagtttcct tactcatttg ggattgcagt
    17521 tttttctaat tctgttttta ttgcctcatt agcttttaaa gctcgttgtg caattgcttc
    17581 atatcttgct tttttgtctc gaatgcgctc tggcagttca ggaaacagac catagttggc
    17641 attcatcggt tggaaatgtt ttccttccgc atgagtaatg tagtaagcca tacttccaat
    17701 agctgtttct tgtggaaact cgactggttc catttctttt gctaagcgtg cggcgttgat
    17761 tccagcaagt agtccgctcg ctgcactttc aacatagcct tctacaccag tcatctgacc
    17821 tgcaaagaac agatcttcgc gtttcgttga ttggtaagtc ggtttcagca attctggcga
    17881 attcataaag ctgtttcggt gcatcacacc atagcgaacg aattcagcat tttctaaccc
    17941 aggaatcatc cggaaaacac gtttttgctc gccccatttc aagtgagtct gaaagcccac
    18001 gatgttgtat aaagaagctg cagcattgtc ttgacgcagc tggataacag cataaggacg
    18061 tttgccagtt ttaggatctt ccaatccaac tggtttcata ggtccgaaaa gcatcgtttt
    18121 gaatcctcgc tgtgccatta cttcaattgg catacatcct tcaaaatatt tttctttctc
    18181 aaaagattta agttctgcca cttcggcatt gattagtgcc tcatggaaag ctttgaattc
    18241 ctcttctgtc atcggacagt tcaagtaagc tgcttctcct ttgtcatagc gggacttcaa
    18301 atatacttta tccatatcga tcgtagcttt gtctacgatt ggtgcagctg catcatagaa
    18361 ataaaaacca tctgaaccat tgaatgcttt gatgctttct gctaatggat gagaagtcaa
    18421 aggtcctgtt gctactacgg tgattccttc cggtatttca gtaatttctt cgtttatcac
    18481 tgttactaat ggatgctctt tgacttttcg ggtaattgtt tcagaaaaag aatcgcggtc
    18541 aacagctaag gcaccaccag ctggcactgc tgtttcatct gccgaagtga ttactaccga
    18601 attcaatcgg cgcatttctt ctttcaataa ccctactgca ttagccaaac tgtttcctct
    18661 taatgaattg gaacatacta gttcagcaaa attacttgtg tgatgcgcct cagtcgattt
    18721 gactggacgc atttcatata aacggacagg gactcctgct tgtgcgatct gccaagcagc
    18781 ttcacttccg gcaagccctg ctccaacaac atttaccatt ttcaatgaaa gaacctcttt
    18841 tctattattt ttggatgtct tcttcgtaat ctccatttac acagactact tgtttaccgc
    18901 ctttgacttt cttctcgacc agatatccgt cacattttgg acaatttctg cccactggtt
    18961 tatcccaaga agtgaattca cattctggga aacgggaaca accatagaat agtctgtttt
    19021 tcttagattt tcgttcaatg acttgtcctt catgacatac tgggcaagtc acaccgattt
    19081 cttttacgat tggtttcgta ttccgacaat caggaaaatt actgcaagca taaaacttcc
    19141 catatttccc taattttatc accatcggat gtccacagac atcacaatcg aatcctgctg
    19201 gttcatcttt gatttgaatt ttttcgattt tttcttccgc attcgtcagt tctttttcaa
    19261 acggtttata gaaacgatca accactttga cccacgcttc cgtgccttct tcaattttat
    19321 ccaaatctcc ttccatttct gctgtgaaat ggatatcgac gatttgaggg aagaattcac
    19381 agattaatga attgacaatt tctcctagtt cagtcggctc aaatcttttt tgtgtcaatt
    19441 ttacatagta gcgtctttgg atcgtatcaa gtgttggtgc ataagtggaa ggtcgcccta
    19501 ccccgttttc ttctagtgca cgaatcaacg tagcctcact aaaacgtgct ggtggttgag
    19561 taaaatgttg ttttggttca atatcaacag attggactac atcgccttct tcaagttctg
    19621 gaagaatatt ttctttctct tctttgccgt catctctacc ttccacatac acttgcatga
    19681 atcctttgaa tttgattttt gaaccgttcg caatgaagat gacaccattt tgttctaatg
    19741 tcactttcat tgtatctaaa atagctggtg tcatctgact agccacaaaa cgtgaccaga
    19801 tcaatgtata aagtttcaat tgatctttat ctaaatattg tttgagttca tctggtgtac
    19861 gaagcacact cgttggacgc acagcttcat gggcatcttg tgcgccttga gcatttttga
    19921 tctttcggtg atcatgtcta gaaaattctt ttccatacgt atcttcaata aatgctgcgg
    19981 cctctgcttt tgctgtatcg gcaatccggg tagaatcagt acgcatgtat gtaatcaagc
    20041 cgactgttcc ttgtttaccc aaagtgattc cttcatataa ttgctgtgct accatcatgg
    20101 tttttcttgt tcgaaagttt aacttacggg cagcttcttg ttgcaggcta cttgtagtga
    20161 aaggattcgc agggtttctt ttgcgctctt tcttttctac ttttttaact tcgaagtctg
    20221 ttccatcaat tctagcagtg acttctttta cactttgtgc atctggtaat ttctttttct
    20281 taccgtcgat tccccagaaa tttgctttga atttcttgcg agctttttta aaattaccgt
    20341 cgatgctcca atattcttct ggaacaaatt ctcggatctc tttttcacgg tcaataataa
    20401 tttttaaagc gacagattgg acacgtcctg cacttaaccc tttcttgacc ttacgccaga
    20461 gaataggact gatcgaataa ccaacgattc tgtctaaggt acgacgagct tgctgtgcat
    20521 ctactaaatc tacatcgatc gttcttggtt ccttaaaagc tgctttgact gcgtcttttg
    20581 tgatttcatt gaagaccacc cgattttttt ctttcagatc caacccaagt aggtacgcta
    20641 aatgccaagc aatcgcttct ccttctctat ccgggtcact tgccaagtaa actttttgag
    20701 ctttttttgc tgcggctttc aggcttttga tgacatcgcc ttttccgcgt atagaaatat
    20761 aatggggttc ataattgttt tcaaaatcga tacccatttt acttttcggt aaatctctta
    20821 tatggccaac actggccatt actttatagt ttcgtccaag atatttctca attgtcttgg
    20881 cttttgcagg tgattctaca atcactaaat atttatacgc cattactgaa tggactcctt
    20941 tccattgtcg tgttcctact attaaaagag gattcataca aatcgtagct aatcatatcc
    21001 attcagaaaa cgtttgtcaa gaaaagcaaa tgaaaagtat ggtgttattc tttaaattct
    21061 gccaagatat cttcacccga aaccgtgcaa actgcaccgt cttggatcaa atgatgacat
    21121 ccggtagaat gttctgttaa aatatttcct ggaacagaaa aaactgttct tccaagatcc
    21181 aatgcttgct gtgctgttat cagagagccg ctttttttct tggcttcaaa gacacatgtt
    21241 cctttagaca aagctgcgat aatccgattt ctcattggaa aacaatgctt tttcgggccg
    21301 gattgaggag gatattcact caaaagaaga tgatcatttg caatcacctc ttgcaagtca
    21361 cgattttcag caggatagac ttgctctatc cctgttccaa tgactgcgat tgtccgtcca
    21421 ccatgagaga ttgctgcttg gtgaccgtat gtatctatcc ctttcgcgca gccactgacg
    21481 atcacatatc cagcttctat cactggtttt agtaaataat aaactgtttt tttggctaaa
    21541 gcagaagtgt gtcttccacc aacaacgctt aacatgttac aagaaagtaa agatagatca
    21601 ccattataaa acagtacaat tggcggatag gtcaagtgaa gcagctgagg gggatatttg
    21661 ggatcaaagc aagtgataaa cgactgtttc gattggattt tgccccagtt agcgtgaatt
    21721 tcctgccaac ttttgcgaaa cagctcccgg tgtttggtta ttttacctat cttaatgatg
    21781 tcttcaaatc cgatttcttt ataaggatac tgcatcatcc agttagcaag tgcccatttt
    21841 ccttgactac tgattccttt tgtataagca agtttcaata aattttcttc tatttgatac
    21901 atcataaaac cgcctttcgt ctctctttct aataagatac gtaaaagcgg ctgtttttta
    21961 ttgtaaatat ttttttacag gtgcaaaact tttacgatgg attggcgtaa ttccttgtcg
    22021 ttcaagtcct tctaagtgtt cctttgttcc ataacctgca ttatgcgaga aaccatatcc
    22081 aggataaagc acgtcatatt ctttcatcaa ttggtctcga tatactttag caatgatact
    22141 tgcagcagca atcgacacag atcgtgcatc ccctttgatt attttttcct gctgaatagg
    22201 caaatccaag gtcatcgcat cgatcaacaa atagcttggt acaatggaca atttttctac
    22261 tgcttggatc atcgctaatt tacttgcttc ataaatattc acatcatcga ttgtctgctg
    22321 atcaacgatc ccgatcccga ttgccagggc tttttcttga acttgttgat aaagaagttc
    22381 tcgctttttc tctgataatt gtttggagtc attcagccct aatatgatct catttttcgg
    22441 caagataact gcagctgcca cgacaggtcc agctaatggt cctcttccta cttcatcaat
    22501 gccggcaatc cattggtgac cttgtgcgta cccttgcttt tcaaagcaaa acatttcttc
    22561 atatttttcc ttaattaatg cttccttttt caatcgtcgt tcaaaagaag ccagtgcttg
    22621 ctgcacgcct ttgcgtggat catttcttaa tgattctagt gtaggatcat ctattctctg
    22681 tatgcttttc aatcgttctt tgatactttg gatggattca gtcatcactt ttttgctcca
    22741 tttcttcgca acgatccagt gtgtatcgtc caagttttcc ttgacgaata tcatgcacga
    22801 ctaattcgct gccacgatca tagtcttctt tatacccacg ttttgctgta atatccatca
    22861 gtaattccgg tgccaatttt tcttcttgct cgattgataa accataacga tcgctgattc
    22921 ttcctgaata atatcgggca aaaaagtcta atccgtaaat cgctaaatca tctaaatgga
    22981 caagctggtc tttgatcgcg cctgtcagcg ctagtttttt gccgatttct tcatcttcaa
    23041 atttgggcca taaaatccct ggtgtgtcta acaattccaa ctcattacct aaacgaagcc
    23101 actgctgacc ttttgttacg ccaggcttat ttcctgtctg tgcaattttc ttgcctacta
    23161 gtcgattcat caacgtagat ttcccaacat ttggaatacc taagaccatt gcccgaatag
    23221 cgcgaggctt gatcccccga gcagcatctc ttgcccgttt ttctttcaat gcttctttag
    23281 ccgcaggtac tagaccttta attcctttac tttcttgtgc cgtgatagct aatgcaagat
    23341 ggccttgttt acgaaaatat tcttgccaca gctttgtctg ttgtggatct gccagatccg
    23401 ctttattcaa taaaacaagg cgaggttttt gctgaatgat ctgatctagc attggattac
    23461 gagaagataa aggtaatcgt gcgtctacca attcaaatac gatgtccaca tatttcattt
    23521 tttcagaaac ttctcgtcgg gcttttgcca tatgtcccgg aaaccattgg atggtcattg
    23581 ttctctccat tcctttcata ccggaatatt ccccggcttt acttgttttt ttctttttca
    23641 atataaattc acttttttat tataaagaaa ttccatctat tagttaagtg aaagtgaacc
    23701 agtcctcatt ctatcacatc ttcacttttt tgatgatgca aatttttatt ttggtttcta
    23761 tatatgaatc taaatttttg acagcgtttc ctaaataaga gatgatagac gaacaaaaca
    23821 gaaaggagac gaaacaaatg aatagtatgg tttttgttaa ttttcctgtt gctgatgtca
    23881 atcgttccgt tgctttttat gaaaagctgg gttttaaaaa aaatgaagaa ttttctactg
    23941 aagaagctgg ttcaatggta tgggatgaca ctttttggat aatgcttctc tctcatgatt
    24001 tttataaaaa atttataaaa gataaagaaa tagctgataa taaaaaaaca agtggtgctt
    24061 tgatttcgtt tagtgttgaa aatgcggatg aagtaaaaaa atttgctcaa attgcaaaag
    24121 aaaatggtgg tactttccac gaagtagata tgggaatgcc tgaagaagaa atgttttcat
    24181 tagaagtcca agatctagac ggaaatacat tagaacccgt atggatgaag atgtaagcag
    24241 ataactaatc tgacgttttt tataacaaaa aaacaaaggc ggatgatacg ataagtattc
    24301 tccgcctcta ttttttttat tcgtacaatc tcttttttac agtttataaa agcagtcttc
    24361 tcaccctttt tttaattgtt agatgccagt gtatgtaaat caccaatgtg tcatgacaca
    24421 ttggtgattt tatggtatcc ttttattatt aactgacaaa ggagagaata ttttgaacac
    24481 tttgattgca aaagacgttt ttagattgcc cgaaatgaat gcaagattgt tgtcgacctc
    24541 tgctctttta ttagcccttg atgtcatctt atacaagata gcgattggtc ctgcttattt
    24601 tcaaatcaca ttcggatttt taagtatggc gcttatgggt tatctatatg gaccgatctg
    24661 ggctggtttg cttgaaatgt tgtcgaacac tttaaatttc acgatatttg gttcaggaac
    24721 gtttcaagct gcttttctta tcacggcttt ccttggtgga tttattaatg gcttattttt
    24781 atatcataaa aagataaacc tgccttccgt catcgctgcc caattattga ttatgatccc
    24841 agtcagcctt tttatcaatt cttggctgat gtctgttttg tttggtgcag atttcaaagt
    24901 aatttttcta ggtagattca tcaggaatat gatccaaatc cctattcaaa taatggttgt
    24961 ttattcattt atcaaaactt tagaggctag aaaagtgttt aaacgagctg tttgatttta
    25021 cgtactaagg tagtaaactg attttggatt aaacaataaa gcgtgcagtc tcaacagact
    25081 gcacgcttta ttgtttctca ctggatccat tttatttcat caagtggata atagacaaat
    25141 cttgctttac ctagaatatc agttttattg atcgtgccaa acgaacgact atcttttgaa
    25201 actcttcgat tatctccaag tacaaaataa ttatcttccg gaagcttgga ttgtcccgtt
    25261 agctcttgta gtgtaaagtc cgtcgtatag gatgcatttg catgatctga tttcaggttt
    25321 ttagttagat aaggttcctt aactactttc ccattgatct tcaattgatc attttggtag
    25381 ctgacatttt ctcctggaag accgattact cgtttgatat aaatcgtgcc atctgctaat
    25441 tgaaaaacaa caatatcaaa tcgtctgatc tccgaaaatt tttccatcac aaccatatca
    25501 ccttgcttca acacgttttc cattgaattg ccttccactg gtaccgggat gaataaaaat
    25561 ccccgcaaca tgaacgcaag gaatacagaa acaagtaagt atttgaatac aagccagaag
    25621 cgattaatat agcgttgttt ttttgtcaat ctctatcaat ccatccctct ttttttcatt
    25681 ctttctttat tataagaaaa atatcaaaaa aatgcgacta gatagctgaa ataaaaataa
    25741 ttctttcaat taggctattg gacaaaaaac ttttttgatc ataaagtaca aaactatctc
    25801 ttatatgtca tgattatttt ctaaatattt ttcccatgct tcgtcaaaga tatccatgga
    25861 cggcaaatag tcgacattca gttccagata attagaaatt tcttcgtagc tagagctgtg
    25921 cttaggaaat tggatgtctt taccggcttc tgtagcgaaa acagataaag catcatgtgt
    25981 gccaccgcct cgcagtgtca ttaaataatg aaaaaagctt cgattcaccc ttcgttcctc
    26041 caattttctt cgataaaggc agcccggcgt gcatggtttc ttttaaaatt ttcaggatgc
    26101 tttttataat agtcttgatg gtcttcttct gctagataaa atggttgggc tggttcgatt
    26161 tttgtaacga ttggctgttc gaaacgtcca ctttcagcta actgtttctt acttttttca
    26221 gcaatgatcc gctgttcttc tgtcaaataa aagatcaccg ggcgataatt gtcaccgcga
    26281 tcctcgaatt gtccgaaagc gtcagttgga tcagtttgct gccagtaaag ttctactaat
    26341 tgttcatatg aaatcaatgt tggatcatag ataatctcca ctgcttctgt atgtccagtc
    26401 gtttggcttt ttacttgttc ataagtcgga tctggtacat gccctccagt atatcctgac
    26461 atcactgtat ggataccagg aagcgtgtcg aaaggctgga tcatacacca gaagcagcct
    26521 cctgcaaata ttgctcgttc cattatgatt ccccctctaa tactactcat ccgatgattt
    26581 tttattttct tttgtcttgc tttcagtact gctggtgcta gttttgttgg tatttgagtt
    26641 gtttgaagca tcagaggtat cttttggcaa atatagacta aaccgaatgt catcatttac
    26701 taagtcgatt ttatctgctt taatatacat cccgttttgc attcggaatt gatccaacct
    26761 aagcatgata gtcagatcat ctgtattgac ttcgatccaa tctggtaatt tatagtttct
    26821 cttgatcata ttcatcacat ctttgattgg caaactcaat gttccgatag aaagacttct
    26881 agcttttagt tggacatttc cgttttccat cacatacgga tcaaaatata aatagaaatc
    26941 gataggaaaa cctagtactt cgaaagtacc attgagtagt gcttcatttt ccaaataaaa
    27001 tttatattca atatcactat ctttttgata ctcactaagg aaaaagctga tgatttgatt
    27061 gactttttct ttatttgaat tgatcgttaa gacaggatct ccttgtcttt ccgtaagagc
    27121 tggtatttct gggagttctg gttcccgatt agcaaaaata cggctgccta aaaatacggt
    27181 cgaaccaagt actaccccaa gaaggataag gaaggcgaat ttccagtaat ttgttttctt
    27241 tgttttgttt gaagatacgt cagatcttgt tttcatgttt attgatcatc ctccttctct
    27301 aaccattggt tttgtgtctc gatcagctga tctctcactg catttgccat caattgataa
    27361 cctaaattat ttggatggaa atgatcttct tcatagagca cattattttt gatgccattt
    27421 agattttctg tcgtgctagt tgtttgatct gttccttctt ctgtgacacc tacttcctga
    27481 ttcaatcctt gataaagcaa atcattgatt gggataaagt agcagttcgg catttttttt
    27541 gtcaccgttt ctgttccttt gttccaattg ttaacaattg tctgcatgtc agtgatctca
    27601 ggaaagttca aataaaatgg attgtagatg cctaaaatgt aaataggtgc ggttttattg
    27661 agggatcgaa attcttccaa tagttctgtg acattttcct gatagttttt cagtggcttt
    27721 ttaaaggaat tgaccgttaa cccgaataaa ttattttgga taactttcat caaatcatta
    27781 ccgccaacag tcagtgtgat aaaatcagct gttttgatat tgtttcgaat ttcttctttt
    27841 gtttttactc gctttaagat ctggtcactt cgttcaccat ttacaccata gttttctgtt
    27901 tcaaccgcgg tcaaattata acggtctttt agatcatttg cgacaagggg aacgaatcct
    27961 ccttgcttgg tttgatctcc tactccttca gtcagcgaat ctccgatagc tatgtagtga
    28021 agtttttctt ttttatttgg atcttctctc acagtagtta ctggtttcat tagtgggcca
    28081 gctttcggta tagaagcagc taaacctgca tagatgatga tcgcaattaa taatgggaac
    28141 aaaacgagga tcaaatgtcg tttcgttaat ttcatttttt cacctcttgt attcgattgg
    28201 ttctaatgtt atcaaaaatt ttatcaaaaa ggtagtcttt atgaaaaaag aaaacaatta
    28261 ttttcctaat tttataaaga aaaagagaat aagctgcaag cctttcaggc tgagggctta
    28321 tcctcttttc gactaatcta cataatacat gattgcaaac gcgtctttac cagtatgcgt
    28381 agcgataacc ggattcgtat gcagtacagg gatatgcatg tccgggaata attcttgcaa
    28441 accctctttg aattcttgcg ccaattcttt tgcatccgca tgtgaaatac cgatttgtcg
    28501 cacatttggc aattctttca gttcattttt caaattatta aaccatttat cgaacgtttt
    28561 ctttcctcgc cccttagtta cagggattaa ttcagaatgg gcgaaattca tcaccacgcg
    28621 tatgttcaaa acactagaca gcaaaccagt tgttcggctg attcttccac cttttaccaa
    28681 gttatctagt gtggatattc cgatgtataa ttttgtattg tcttgtactt ttttgacttc
    28741 ttcaaggatt ttctctactg ttttaccttc ttttgctaat tcagctgctc gaattacctg
    28801 aaaagataat ccttggtcag tggtatcact gtcgataacc gttactttcg tttttgttag
    28861 attactggct tgtcttgctg cttctactgt tccactcaag ccttttgtca tatggatcga
    28921 aatcacttca ctgccgtctt ctcctagctc atcatacatc gagatgaatt ctccaattgg
    28981 tggttggctt gttttgggca gattttttgc tttcgccatc atttccataa atctttcgcc
    29041 gtctagttgg tcatcatcgg gatatactgt tccgtcgatc atgatggata gcggcatgac
    29101 acgaatgcct aaagcatcgc gtacattttt ttccatcgta catgaagaat ctgttacgat
    29161 ttttacattt gtcattattt tctctctctc tttctttaac aagatgcctt attcatgtta
    29221 aactacatgt aagacaattt ctatttttag tataacagac
//
LOCUS       DAHHZJ010000018        28950 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.019, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000018 DAHHZJ010000000
VERSION     DAHHZJ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.019"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            666..2276
                     /locus_tag="NBL02_000655"
     CDS             666..2276
                     /locus_tag="NBL02_000655"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HCD2571363.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            2568..3437
                     /locus_tag="NBL02_000656"
     CDS             2568..3437
                     /locus_tag="NBL02_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HCD2571364.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            3602..4873
                     /locus_tag="NBL02_000657"
     CDS             3602..4873
                     /locus_tag="NBL02_000657"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCD2571365.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            4870..6165
                     /gene="rho"
                     /locus_tag="NBL02_000658"
     CDS             4870..6165
                     /gene="rho"
                     /locus_tag="NBL02_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCD2571366.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            6264..6527
                     /locus_tag="NBL02_000659"
     CDS             6264..6527
                     /locus_tag="NBL02_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HCD2571367.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            6972..7421
                     /locus_tag="NBL02_000660"
     CDS             6972..7421
                     /locus_tag="NBL02_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCD2571368.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            7478..8896
                     /locus_tag="NBL02_000661"
     CDS             7478..8896
                     /locus_tag="NBL02_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCD2571369.1"
                     /translation="MKIVGVTACPTGIAHTYMSVEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            8896..9756
                     /locus_tag="NBL02_000662"
     CDS             8896..9756
                     /locus_tag="NBL02_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCD2571370.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            9930..10703
                     /locus_tag="NBL02_000663"
     CDS             9930..10703
                     /locus_tag="NBL02_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCD2571371.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            10850..11215
                     /locus_tag="NBL02_000664"
     CDS             10850..11215
                     /locus_tag="NBL02_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HCD2571372.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(11363..12571)
                     /locus_tag="NBL02_000665"
     CDS             complement(11363..12571)
                     /locus_tag="NBL02_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD2571373.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(12590..12868)
                     /locus_tag="NBL02_000666"
     CDS             complement(12590..12868)
                     /locus_tag="NBL02_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571374.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            13022..13717
                     /locus_tag="NBL02_000667"
     CDS             13022..13717
                     /locus_tag="NBL02_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCD2571375.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(14159..14956)
                     /locus_tag="NBL02_000668"
     CDS             complement(14159..14956)
                     /locus_tag="NBL02_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HCD2571376.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(14953..16137)
                     /locus_tag="NBL02_000669"
     CDS             complement(14953..16137)
                     /locus_tag="NBL02_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HCD2571377.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(16162..17409)
                     /locus_tag="NBL02_000670"
     CDS             complement(16162..17409)
                     /locus_tag="NBL02_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HCD2571378.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            17624..19369
                     /gene="dnaX"
                     /locus_tag="NBL02_000671"
     CDS             17624..19369
                     /gene="dnaX"
                     /locus_tag="NBL02_000671"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCD2571379.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            19389..19703
                     /locus_tag="NBL02_000672"
     CDS             19389..19703
                     /locus_tag="NBL02_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCD2571380.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            19728..20324
                     /gene="recR"
                     /locus_tag="NBL02_000673"
     CDS             19728..20324
                     /gene="recR"
                     /locus_tag="NBL02_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCD2571381.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            20388..21032
                     /locus_tag="NBL02_000674"
     CDS             20388..21032
                     /locus_tag="NBL02_000674"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCD2571382.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            21047..21376
                     /locus_tag="NBL02_000675"
     CDS             21047..21376
                     /locus_tag="NBL02_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HCD2571383.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            21390..22328
                     /gene="holB"
                     /locus_tag="NBL02_000676"
     CDS             21390..22328
                     /gene="holB"
                     /locus_tag="NBL02_000676"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCD2571384.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            22364..23188
                     /locus_tag="NBL02_000677"
     CDS             22364..23188
                     /locus_tag="NBL02_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HCD2571385.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            23181..23528
                     /locus_tag="NBL02_000678"
     CDS             23181..23528
                     /locus_tag="NBL02_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HCD2571386.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            23597..24469
                     /gene="rsmI"
                     /locus_tag="NBL02_000679"
     CDS             23597..24469
                     /gene="rsmI"
                     /locus_tag="NBL02_000679"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCD2571387.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            24578..25699
                     /gene="dinB"
                     /locus_tag="NBL02_000680"
     CDS             24578..25699
                     /gene="dinB"
                     /locus_tag="NBL02_000680"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCD2571388.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(25753..26355)
                     /gene="nrdG"
                     /locus_tag="NBL02_000681"
     CDS             complement(25753..26355)
                     /gene="nrdG"
                     /locus_tag="NBL02_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HCD2571389.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(26670..28859)
                     /gene="nrdD"
                     /locus_tag="NBL02_000682"
     CDS             complement(26670..28859)
                     /gene="nrdD"
                     /locus_tag="NBL02_000682"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCD2571390.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
BASE COUNT         9299 a         5214 c         6083 g         8354 t
ORIGIN      
        1 ttttgtcgca tctccttatt ttttacttat gtgattcgtt accttcaatg aatccctaca
       61 aggcactttt gttgaaaaaa aggtcatctt tgaaaaaatt agtggaattg ttttgttttt
      121 gatattattt aatagaaaag atatgccaaa aatccgagaa ctgtggacta cgttttttac
      181 gtaaaggagg taaataaggg tgaaagaaaa agattgtttc tacgataagc tatataaact
      241 gttgctgcaa gtaggaaaaa gtagttcttt gttaggtggg atattatcta tcataatgtt
      301 tgttttattt ttttatctat tatttcactt aaaatttttc agtaacgatt ttctgatttg
      361 cttaggttta acgttagcta tgtttatatc aagcttatta tgtgggttaa attttttttg
      421 cactcataaa ggacttaaga aataatttct caaaggatat cgaaaaagaa aaaattgtaa
      481 gcggatagta ttcatttgag tacttgactt tcccttagtc agcttgtatt atacaccttg
      541 ggcacccttt tagagggtca gaagtttaca gaatttcggc tccctattct tattgaagaa
      601 tagggagctt tgtttattgt ctgagacttt tcctgcacat aaattaaaaa ggagtttttg
      661 aacttatgac aaaatatatt tttgtgaccg gcggtgtcgt ttcatcaatc ggaaaaggga
      721 ttgttgcagc ttctttagga cgcttattga aaaaccgcgg cttaaaagta actatccaaa
      781 aatttgatcc atacatcaac gtggatccgg ggacgatgag cccatatcag cacggagaag
      841 tattcgtaac agatgatggt gctgaaacag atttggatct gggccactac gaacgtttta
      901 ttgacatcaa tttgaacaaa tactccaacg tgacgacagg aaaaatctat tctgaagttt
      961 tgcgtaaaga acgtaagggc gaatacttgg gagcaactgt tcaagttatt cctcatatta
     1021 caaatgagat caaagaaaaa atcatgcgag cagctaaaat gactgattca gatgtgatca
     1081 ttacagaagt agggggaaca gtaggggata tagaatcttt gccattcctt gaagcacttc
     1141 gtcaaatgaa agcagatgtc ggtacagata atgttatgta tatccataca acattgattc
     1201 cttacttgaa agctgctgga gaaatgaaaa ctaaaccaac gcagcacagt gtaaaagagc
     1261 tacgtggttt aggtatccaa ccgaatattt tggtggtacg tacagagcaa cctgtttctc
     1321 aaagcgtgaa aaacaaattg gcacaatttt gtgatgtcga accagaagca gttatcgagt
     1381 cgccagatgt agatacactt tactccatcc cattagtttt acaagcacaa ggaatggatc
     1441 aaattgtttg cgaccattta aaactagata caccagaagc agacatgaca gaatggaaag
     1501 aattagaaga acgtgtcttg aacttgaaga aaaaagtacg gattgcgctt gtcggtaaat
     1561 atgtcgagtt accagatgca tatatctcag tagttgaagc attgaaacat tctggtttcg
     1621 cctataattc agacatcgag atcgattgga tcaaagcaca agaattaacg agagaaaatg
     1681 tagaagaacg tttgaaagac gcagatggaa tcttggttcc tggtggattt ggtgatcgcg
     1741 gagtagaagg gaaaatcgaa gcgattcgtt atgctcgcga aaatgacgtt ccgtttttag
     1801 gtatttgtct tggtatgcag atggcttgtg tcgaatttgc acgtaacgtc gttggattag
     1861 aagatgcgtc atcagctgaa acgaatccag atacagcaaa caatattatt gatttaatgg
     1921 ctgatcagga aaacatcgag aatttgggtg gaactttacg ccttggatta tacccatgta
     1981 aaatcaaaaa aggaacgaag acggctgaag catatgatgg agccgatgtt gtgcaagaac
     2041 gtcaccgtca ccgctatgag ttcaataaca aatatcgtca attgtttgaa gaacaagggc
     2101 tagtcttttc aggtgtctca ccagataacc gtttagtaga aatcgtagag ctgccagaga
     2161 aaaaattctt tgtgggatgc caattccacc cagaattgat ttctcgtcca aatcgcccgc
     2221 aaaaattaat caaagcattt gtcggagcat cattagcaga tcacgaaaca aaataatttt
     2281 tgaacttgat agaaagagaa aagtagcaag agaaggggat aagcaatttt cttgtcactt
     2341 ttctcttttt ctgatttgtt attatttgta ggaaaaaaga tagaaagcaa aagacttttt
     2401 tcgattaaag cgctattgga tattttattt tttgcaaaaa acttcttgaa aattttcatt
     2461 ttcattgatt ttagtagaaa actaccgaaa tgtttggtaa aatagggtcg ttggttaaga
     2521 attgtcttaa caaatttagt tttacaaaac ttaggaggaa ttttattatg ccagtagtat
     2581 caggagctga atttttaaaa gctgctcgta aaggcggtta cgctgtcggc ggattcaaca
     2641 caaacaactt ggaatggact caagcaatcc ttgaagcggc tgaagcaaaa caagcaccag
     2701 tacttatcca aacatcaatg ggtgcagcta aatacatggg tggttacaaa atctgtaaag
     2761 atttaatcac tgatttagtt gaagcaatga acatcactgt tccagttgct atccacttag
     2821 accatggtga ttacgaagct gctttggaat gtatcgaatt aggctatact tcagtaatgt
     2881 tcgatggttc acatctacca tttgaagaaa acttgaaatt agctaaagac gttgttgaaa
     2941 aagcacatgc aaaaggtatc tcagttgaat gtgaagttgg ttcaatcggc ggagaagaag
     3001 acggaatcat cggtaacggc gaattagctg atattgaaga atgcaaacaa atggttgcta
     3061 caggtatcga ctacttagct tgcggtatcg gtaacatcca tggtcaatat ccagaaaact
     3121 ggaaaggatt agcttttgac cacttacaag caattgctga tgctgtaggt ccagacatgc
     3181 ctttagtatt acatggcgga tcaggtattc ctcaagatca aatcgaaaaa gctatctcaa
     3241 tgggtatctc aaaagtaaat gttaacactg aattccaatt atcatttgct gcagctactc
     3301 gtaaatatat cgaagctggt aaagataaag aaggtaaagg attcgaccct cgtaaattat
     3361 tagctccagg taaagcagca atcgttgaag atgctaaatc tcatatcgac tggttcggtt
     3421 cagctaacaa agcgtaagca tccacataac agatacgata tataggaatg gagaacgact
     3481 tttgaagtcg ctctccattc ttttttactt aaaacggcag accatgctcg tttctttttt
     3541 ttatctatga taaaatacaa gcatatccat gaaaaaagat taataaacaa ggtggaaaca
     3601 gatgaaaaaa atcgtaatca aaggaaaccg acctttagct ggtgaagtaa cgatcagcgg
     3661 tgcaaaaaat agtgcagtcg cattgattcc agctgcaatc ttagctgatt ccccagtgat
     3721 cttggaaggt gtgccggaca tacaagatgt tcattcatta attgaaattt tagagatcat
     3781 gggcgcaact gtccattttt ccaacaacat actagaaatc gatcctcgag aagtcgtatc
     3841 gattccgatg ccgcacggaa aaatcaatag tttgcgtgct tcttattatt ttatgggaac
     3901 actgcttggt aaatttggcg aagcggtagt aggcctgcca ggtggctgtt atttaggccc
     3961 tagaccgatt gacctacacg tcaaaggatt tgaagctcta ggtgctgaag tgacaaatga
     4021 acatggtgcc atgtatttac ggacagaaaa taaaggattg cgtggcaatc ggattttcat
     4081 ggacgtggtt tcagtcggag caacgatcaa tgtgatgtta gctgctgtaa aagcaaaagg
     4141 acagacagta attgaaaacg ctgcacgaga accggaaatc atagatgtgg caactttact
     4201 gaataatatg ggggcaaaag ttcgaggagc gggtacagat gtgatccgta tcgaaggagt
     4261 cgaaacatta catggctgtc ggcatttcat gattcctgac cgcatcgaag caggaaccta
     4321 tctagcatta gcagctgcag taggaaatgg tatcaaagtg aaaaatgtga tctttgaaca
     4381 tttggaaagc tttatagcaa aattgcagga aattggtgtc catatgaaaa tcagcgaaga
     4441 tgaaatcgaa gtttacccct cgaaagaatt aaaacccgca aacatcatga cctatcctta
     4501 tccaggattt gccacagatc tgcagcagcc acttactgcg ttactattga tgactacagg
     4561 cacttcagaa atcatcgata cgatctatgc gaaacgtgtg aaccatgtgc cggaactagc
     4621 acgaatgggc gctgatatta ccgtggaagg aaatatgatc atcgtgaatg gtccaaataa
     4681 acttcacggc acagaagtcg tggcttctga tcttcgggca ggtgcttgtt tagtgattgc
     4741 tggactattg gcagaaggga caacgacgat ttacaatgta gattatatct tacgcggtta
     4801 tgatcatatt atcgagaaac taacagcctt aggggcatca attgaaatga tagaggaagt
     4861 gggagctgaa tgagcgatta tttgacaatg gcagaactag agaacaaaac gttaaaagaa
     4921 gtttatagtt atgccaaaga atttaaaatt ccttattaca gtaagatgaa taaaaaggaa
     4981 ttatctctag ctgtgattcg tgcacaagca gagaaacaag gattttatta tatggaagga
     5041 atattggata tcgttggtca agatggttac ggttttttac ggccaatcaa ttatggacca
     5101 agtgcagaag atatctatat ttctgcctca caaatcagac gattcagttt gagaaatggg
     5161 gacaaagtgg caggaaaagc ccgtccacct aaagaatcgg aacgttatta tggtttgatg
     5221 cacgtggaaa gcgttaatgg aaaagatccg gaagaagcaa aagaacgtcc gcattttcca
     5281 gccttaacac ctctttatcc gcaaagacag cttcaactgg aaacaacacc gcaaagacta
     5341 tctacccgaa tgatcgatat cttttctcca atcggttttg gtcagcgtgg attgatcgtc
     5401 gcaccgccaa aagcagggaa aacttctgta ttaaaagaaa tcgcaaatgg tatcaccgaa
     5461 aactatccgg aagtagaatt aatcgttcta ctgattgatg aacgaccgga agaagtaacg
     5521 gatctagaac gaagcgtgaa aggcgacgtg gtatcttcta catttgatct gcagccgcaa
     5581 aaccatactc gagtttctga attagtattg gaacgcgcaa tgcgtttagt cgaagataaa
     5641 cgagatgtcg ttatcttgat ggatagtatt actagacttg ctcgggccta taacttagtc
     5701 gttccaccaa gcggacgtac attgagtggc gggattgacc cagccgcatt gtataaacct
     5761 aagaaatttt tcggggcagc aagaaacatc gaagaaggtg gcagtttgac gattttagcg
     5821 accgctttag tcgatacagg aagccgtatg gatgacgtga tctatgaaga attcaaagga
     5881 actggaaatc tagaattaca actttcacgt gaattatctg aacgtcggat cttcccagcc
     5941 atcgatatca aaaaatcagg tacccgaaaa gaagaattat tgatgccaag tgaagaatta
     6001 gaagaaattt ggaaacttcg aaaaaatatg actggagatt cattagaata tacggaacaa
     6061 ctgatccgct ttttgcgtaa aacaaaaaac aatcagcaat ttttcaaaga atttcaagat
     6121 gtctcctttg gtaaaaaaaa tccgaccaga aatctgaaaa gataattgca accttgactg
     6181 aaacatgtta agatgttaat gttgtaaatc gacaaatcaa ctctggttca gcaaatggag
     6241 cagggcaaag gagcgaaaaa atcatgaaag aaaacatcca tccagattac cacccagtag
     6301 tattcatgga ttcaacaact ggtttcaaat tcttgtcagg ttcaacaaaa acttcacaag
     6361 aaacagttga atgggaagac ggtaatacat acccagtcat ccgtgtcgaa gtaacttctg
     6421 actcacatcc attctacact ggacgtcaaa aattcacaca agcagacgga cgtgtggacc
     6481 gtttcaacaa aaaatacggt ctcaaagacg caaacgctgc ggaataataa aaacgctgat
     6541 aactcaacgt ttagcagact atctgatagg gatagtctgt ttttttatgt tttttgccaa
     6601 tttaaaactt cgtttgatgc tgaaaaagtc gtaggatcat aatgcacaaa acaatgaaaa
     6661 actagtatat agttttaatt atccacttga taaaaacacc ttatattaac gaaatgaaga
     6721 ggggttattt cgaagtaaaa aaccatttac ctaaaattag aagctaagct ttcctttcaa
     6781 ggaaggcttt ttgttttgtt atagacttgc gattgtttgt gagctagatc gttcatttta
     6841 atgacttata cacgcggatg atgggtcttt ttcctctgtt cattcataat attgaacggt
     6901 acagctagcg atgctatact gacctcagtt ccaagaaaac gttaacattt tttgtggggg
     6961 agaggttttt tatgaggttt gatcaattga tcgcagaaga actgattgtt ttcgatccat
     7021 cgatagattc aaaaggatca ttatttgagc gaatagggga tgtgttgaaa aaaacaaata
     7081 aggtaagaaa tgttcaaaaa gtcgttaagg cactattcaa acgagaggag gagatctcca
     7141 caggaataga agatggattt gggattcccc atgcaaaaag caaatatgtg acaaaaccag
     7201 cactgtgttt ttttcataca gccaaattaa cggactatag cgggatagat gaaatgccaa
     7261 ttgaatatat ttttgccatc gtagttcctg aaaaatcagc agatatgcat ctagagattc
     7321 tcagcacact ctccagacga ttgatgaatg cagattttag aaagggaata aaagaagcca
     7381 ctacgccagc agatatcatc agacagatta gtcaagaatg aaaatagtag agtctttgtc
     7441 cgagtatcga tagtgaagaa aaaggaggaa aataatcatg aaaatcgttg gtgtaactgc
     7501 ttgcccgact ggaattgctc atacttatat gtcggtagaa aaattagaag taacagcaag
     7561 aaatctaaac catcaagcaa aattcgaaac acaaggtgta aaaacagaaa atcagttatc
     7621 tgaacaagaa atcaaagagg cagatgcaat cattcttgca gtagataaag aaatcgaatt
     7681 ggaccgattt gccgggaaaa aagtaaagcg tgtctctaca agccgagcaa tcaaagaacc
     7741 gcaagtagta atagacgaag cattgagaga tattggcgta ttcgttgtgt ctaatgaaaa
     7801 agaaccagct gcaaatgaga aaaagccgac aatctataat catttcatga atggtgtgaa
     7861 ttacatgctt ccatttgtga tcgcaggcgg aatcatcatc gcactgagtt ttgcctttgg
     7921 gattacagcg gctgatccca actcagcaga ctataatgtg ttagcagctg cattttcaag
     7981 gatcggagga gatacagcgt ttgccatgat ggttcctgcg ttaggcgcgg ggattgctac
     8041 atcgattgct ggaaaagcag gttttgcccc aggaattgtt gccggattac tggcctctac
     8101 aggtggttca ggttttcttg gcggaatgat tggtggcgtg ctagcagggt atatctgtga
     8161 ttttctggca aataaaatta gcgtaaaaaa agaattttct gctatttatc agctgattgt
     8221 cgtccccttt ttatcgatct taattattgg gcttttgatg gtatttgtga ttgaacaacc
     8281 gatcacatgg gtgttggatg ctttgacaaa ttggctgaat agcttgggaa atacatccgg
     8341 gctattgttc ggattactta tcgggatcat gatggcagct gatatgggtg ggccgatcaa
     8401 taaatcgact tcgactttct cgatcggttt gatgtcagct ggagtaaatg caccgattgc
     8461 tgcttgtatg gctgcaggaa tgacacctcc cctaggtatt gctttagcca ctttattatt
     8521 caaacataaa tttaccaaag aagaacggac ttcaggtcaa tcatgctgga ttttaggcgc
     8581 ttcgtatatt acagaaggtg ctataccgtt tgcggtatct gacccgatcc gcgtgatacc
     8641 tagtttgatg acaggttcag cagtcgctgc agggatttcc atggcagtag gagttacttc
     8701 aatggcacct catggaggga tttgggtgat gctaattcca aatgttatga acaatttacc
     8761 tatgtatgtc ttggcaattg catcaggtac agtcactaca gcctttatgc ttggactatt
     8821 gaaaaaaaca gtgaaagaga aaaaacaaga caagtggacg aaaaatgaaa atctaacaaa
     8881 tgaggtggaa gcgtaatgtt atatacaatg aaagagttat tgaaagtggc taaagagaat
     8941 caatttgcag taccagcctt caatatttgc agctatgata tgatgaaagc aatcatcgaa
     9001 gaagcagaga gattagaagc accagtaatc ttagaaattc atcctgatga aatcgcctat
     9061 ttaagtgatg agtttgttgc ggcagtaaga gcctatgctt atacaagtaa actgccgatc
     9121 gttatccact tggatcatgg cggtacgata gaagatgtct tacgggcgat tcgaaacggc
     9181 tattcttccg tgatgatcga tgcgtctttg aaatcattcg acgacaatgt acagattacg
     9241 aaaaaagtgg ttgcgttagc tcatgcggta aatgtctcag tggaggctga gcttggaacg
     9301 attgggaata atggttctgc tgaaggaggt gctgaaacaa tcatttatac agatcctgat
     9361 caagcgaaga tttttgtgaa agaaacagga atcgatacac ttgctgtagc aattggaaca
     9421 gcgcatggct tgtatccgaa aaacagaact ccaaaattaa atatcgactt actgaaagaa
     9481 ttgaaccaac aaatcgatct tccatttgtt ttacatggcg gttcaggtaa tccggacgca
     9541 gaagtcagcg aagcagtaaa atacggagtt gccaaagtga atttgagctc agatcttaaa
     9601 agcgtctttt tcaatgcttt gagagaaata ttgagtgaaa atccatcgat gtacgagccg
     9661 aatgcaattt atccagcagc taataaaaag gtacaagaag tggtcaaaca taaaatggaa
     9721 attttgaaca cagtaggcaa ggctagttta tactaaaaat aaaaatgtgt ggtcaagagg
     9781 gtatgacaat agtcttgtca taccctctta accacacacg atgttcagaa gtagtttttt
     9841 cgaaaaattt cttcttattt ctcgaagcca atcacttctg tcacaacctc ttttttcctg
     9901 ttactatata aagtaaatgg ggtgatttta tgttgaatga agtggagaat agacagcagg
     9961 agattgtgaa ttatctaaaa aacaaccaat ttgcaagagt caccgatttg atcgagttag
    10021 taaattatag cgaagccacg attaaacgtg accttgtttt tctggaaaaa agtggattga
    10081 tccgtagagt tcgcggtggc gccatgctta ttgacaatcg aaaaattgac gtgccttacc
    10141 tgatgaagat caccaaatta gaagaagaaa cagaaaaacg taagattgca gatacagctt
    10201 cttctttgat tcgagatgat atggtcttat tcttggattc cagtacaacc tctttacact
    10261 tagtcagaaa cctagcaaga tttgatggtt tgcagatcat tacgaatggc atcgtcacag
    10321 ctgctatgtt gagtgaatat acaacggcaa aagtatctgt attgggaggg gcaattgttc
    10381 cgaaacgagc aacaatcaac ggttcaaaag cctataacga tgcattgtca tattatgcgg
    10441 atttagcgat aatgagttgt cgcgggctag atttcagcca aggagtaaca gaaactcatg
    10501 aaggagaggc aatggtcaaa aaagctttta gaaaacaatc agaccggctt gccgttttag
    10561 ttacacagca aaaaattgca cataaattca tgcatcaagc cattgctttt catgaaatcg
    10621 attatctggt gacaagtaaa gaactgacaa aagaacagta tgcgaaagta aaggaacacc
    10681 atatcgaatg tctgtacgga taaattatta cgtgaatgtt ctaagtggct tcctcatttt
    10741 tagataggac cttatctttg ctcgcttctc tcagccgatg gtataataat gaaaaggtta
    10801 tcttgttaga taatcttact tttttatcca ggagaaagga gtgcagaaaa tgaaacatgt
    10861 gaaggcgttg gtagtgaagg caatcatgat ctgggcgatt ctgtgggtcg tgctgacagg
    10921 tttatacggt gttagcttta tggattcaac gattgttggg gtaattattg tagtcatgat
    10981 ctatgtgcta ggggatctgt tgatcttacg taaagtaggg aacatcgcag cgacgatcgc
    11041 agatgctggt tcagctgcag ttatcttgtg gctctatcta tattttatga atgatacagt
    11101 cgatatttgg atgaaagtat tgattcctgc tctattgatc ggtgtggcag aatggttctt
    11161 ccacaagtgg ttgctaagtc aaggcatcgt accagatgaa cggaaaatgg aataaatcaa
    11221 cagttaaact attcttatcg ataaatgaaa gctttaaaaa taaatagatg tttaattaag
    11281 aataatcata aactattttt actcaaaaaa ccgctcagat tattttcaga tctgaacggt
    11341 tttttttatc tattcatcaa gatcaaattt catttttcaa tgattcaatc aattcgccct
    11401 ctgcttcatg actatgtttt cggccattaa caagatagag tgctgtacat atcaatggaa
    11461 tgactccggc aatcacatac aatcctctaa atgaagtaag agattttagc atccccatca
    11521 aataaggacc aacgcctaag cctaggtcta aaccaataaa gtaggtagat aaagcaatac
    11581 tgattcgatg ttcgcggacg atttttaaac aaacagcctg tccatttgac ataaacgtac
    11641 cataacctag gccgactaat gctccagaga taagcaatgt cacactattc gtcgttacac
    11701 tcagtaaaaa taatcctgct gttaagaata aatagctagg atacatcacg tattgttctc
    11761 ctttgacatc gaaaatcctt ccagtcaatg gacgtgtaaa tgtgatgacc aaagcatata
    11821 cgacgaagaa aaatgaccca gcagcaacta agtggattac ttttgtataa gaagataaaa
    11881 atgataaaac acttgaataa gaaagaccca tcaaaaaggc aattcctgta atgaaaagtg
    11941 ctttcttttc aataaaacta ttgatattcc atgagcgaag agatattttt tgttcttcag
    12001 ataatggaat attttttaca ggaaagacaa ggcaggcaat cgtaacaaag aagaccagta
    12061 caattgaaaa ccagataatg aaacgaaaat cagtcatatt cagcaaaatc attcctaaaa
    12121 atggcccaac agcggcagct aaactagtac ttaatccata ataattgatg ccttcccctt
    12181 ttttagatgc agggatatat gcagtaacga tagcatttgt agctgtagaa actgtaccgt
    12241 agccgaatcc attcaagaat cgaactaaat acaatgcccc gatagttggc gtataaagat
    12301 atgctaatgt tgaaaggaga tagaagatcg cgccgcctct taaggtaaac ttccgaccaa
    12361 atagttctaa ctgtttcccc atgaataagc gtgccagcaa tgttccgatg atatagatcc
    12421 cagaagcaaa acctgcttgg ctcaagctgg cgtgtaaggt gtcttgcgca atgactgcca
    12481 tgatgaccat caacagataa taaatcaggt agacaataaa attgatcaat gtgattccaa
    12541 taaaatattt attaaataat ttcttttcca tgtatgttat acattcccct taagctgaat
    12601 atgcttttaa tttttgtatt tgaacttcta catcgtgata attggatgga tagcgatcaa
    12661 caagttggcg aataaataaa tctcttgtag acataaacag atttaaaaat tcttcgcatt
    12721 cgtttgttgg attctcatcc actgctttga tcaattcgat cgagttttga tacgcatcat
    12781 ttaaaaagac taaagaagct ttcatatcaa atcttgtttg atatgctgtt tctaatcgat
    12841 taatgattgt gttcatccag taattcatgt tttttccctc tattcttaaa aaattttact
    12901 tctgcagtat aacgaataaa aaaaaggaaa aaaatcgcat aagtgatgga aaaaggtact
    12961 taacaaatgt taatatcatt agtaaaatac ctttggactt taaaaagttt taaagtactg
    13021 tatgatcttt ttagatgaaa gtttggatgg gggagcatca ttggatagta gagtcttaaa
    13081 ggaatatttg gataaaaatg attttccgat tatacgcaaa ggatatcata aatatttgac
    13141 attcgaaggg atggaagatt cccatacgta tatcttgaaa gagggaacgg taaaagcaag
    13201 tgttatttca aatgatggga gagaatttaa ccttcgctat atcaaagatc ttgaaatcgt
    13261 ctctctttta aaagacgaat attcacaatt catcgattcg ccgtataata ttcgagttga
    13321 atcagaatat gcagagtttt atcgaatcaa tcgtgtgcgc ttttggcaaa atatcaacca
    13381 agacccaaag ctacaacact atgtaaaaga gtattaccgc tatcgtctgc tttattcgat
    13441 gaaaaaaatg cagcaaatgc tattaaacgg aaagctggga gctgtttgta cgcaacttta
    13501 cgaattatat gatttattcg gtgtgcggac agaagaaggg atgctgatcg atttttcgat
    13561 cacgaatgaa gaaatcgcca aattttgtgg catctcaact gctagcagtg taaacagaat
    13621 tttaaaacaa ctgaaaaaag atggtattat caccgttaaa gaccatcata tcgttatttt
    13681 gaatatggac ttgttagaag acaatattat cttttgatga acaaagaggt tgtgaaatca
    13741 gcgttttgac ctgagtatta gagaaacaaa cggaaaaata gttcctcata ttttgccgtt
    13801 ttttgagcta ataccgaggg tcagctgatt gaacaccgtt taccaagagg ttgtgagcct
    13861 gccgtttaac tctgagtcac aaggaacaat ttcaaaaaac agcttttcat gtttgatgaa
    13921 tattgtgact tgtgaccgag gagttggtag gagaacaccg tttaccaaga ggttgtgaaa
    13981 aagctgttct gcatcaagta ataagaagag ccaagtaaaa acagtctctt atgtttttaa
    14041 cttggctgga cttatcaccg tagatgtaag cttttgaacg ccgtttattt gcaacaaaag
    14101 gggctgtgac acgatttttg tcacagccct ttttttgcga acattcattt agcaattttt
    14161 agaaacttct gccgccaccg ccaaaagtcc cgccaccgct agaatgggta gtacttccgc
    14221 caccgccgct tccaggaggt gggcttttgg ggattcttct tgtcgtgaca aaagaatttg
    14281 tcagacgatc ggttttttca gttaatttga tactggattt ttctcggaat ggatatttgt
    14341 agatgcctga tttcaattga taacgcgaaa cagtcacaaa ataaaagaca agactaagga
    14401 taagagccaa aacaacagca atgatgattt ctactgtcgt caaaacctta tagcgtgtaa
    14461 ttttgccagt ttcttcgtct acacgataat gtcctcctgg aactccgtct tccacatatg
    14521 ttgctgattt agacaggtaa gcttttgcag cattgtaata ggcagcattt gtcatctgat
    14581 cataaacatc atctaagatc gaatcgattc tgctatcagt caaataatcg atcatattac
    14641 cagaagtcga aatgtagatt tcacgctgtc ccatatctaa tagcaatact gagccatttt
    14701 gatcatttcc gactgcgtct cttaagtaat tatcagcaaa gtctcttggt tcttctgaat
    14761 tagatgtagt cgttacgatc aagactcgtc ctttgatttt ttcattcagc ttttctgcct
    14821 gttgttccaa agattggatt tgatctgttg tgaataaccc agcctcatca tttaccgtag
    14881 ctgtatctgc atgagtagcc accggccagc caacacttac tagcaaaaga aagaataaaa
    14941 aaaatttttt catcatatca agtaacctcc gatcagaaag aggatcgcta gcccagcgaa
    15001 aatcccaaga gcggtcagtc ccaatttttt ctggctgatc ggcaggacac cgctgacctt
    15061 tcctgtttgt ccattcattg cgtagtagta gactttttta cttgagtcat tgcttcgata
    15121 agtaacaagc cataccggca gtaatacgta attatttact tcgtttgtga ttgaagcaga
    15181 agttcgcaca ccggtcaatg ttgtatatcc atttgcggta tcttttaaca gcttctctga
    15241 atagtctttt aattcagatt gaatctgtga tttgattgcc tcatattcga tatctctttt
    15301 ttctgcttgg aacccggcta aatactggct tttaaaatca attgctttat ctaacgggaa
    15361 aggttgcacg ccttcgacca tcttttgttg cgtatttttt gataaggcat tttttactag
    15421 ttctttaaaa gaaagcttgc ctgctcttga aacagcaaat tgcttcgttt ctgtgtattc
    15481 gatgtcacct atccgccata tgcggataac tgtaccattc gcctgcatgg caccatctac
    15541 ttcagcatct gtagcccaat aagggaaata cacacctgtc aacttatcga tttgatcctt
    15601 attgaaaaaa gctttgggaa caaaccattt tttcttggtc caagcgagaa atctgttgat
    15661 tgcttcttct ttctcgacag caaaaggaag tactttattt ggcaagaatt ctccgcttaa
    15721 acgtcccgat agtacgacgg gattatggca gtagtaacaa taagtcgcag cagttgtcgc
    15781 atcagtcacg atctctgcac cacagttggg acaaagaaaa agatctatcg ctgcggtatt
    15841 atctgcagat tcgtccgctg tatctgaaaa accgcctgcc tcgtcaaccg ctttgcgctc
    15901 ggcttcattc atatcggcaa gctgttcttg ggctgtaaaa gtaaaatctt cggtaggtga
    15961 aggttcttct ttgctttctt cgccagccat acgcgcttct ttttgctttt gctcatattg
    16021 gctgacttct tcttctgtat agatattcag gcagtattcg caatgaaatt tttggtcttt
    16081 gggatcaaaa gtcaacggac cgccgcaatt aggacattta tgtgtaagaa catccatcag
    16141 ctgcacacct ttctaaataa attaattaac ggggattcct ttaaacggtt taccacagtt
    16201 tggacagaat ttagggatgc cgttggaaag atccacgatc tcattgcatt cactgcatcg
    16261 catttgtagc ttaggctgaa cgttttctac aggtttggca gctccgcagt tggaacagaa
    16321 ttttccggaa ttttctgtcc cgcaaactgg acatgtccaa gtatccgcat ttccttgtgc
    16381 agtttgttga ttttgttttt ctgcttgttg tttgatttgt tcttggttat tttgagctgc
    16441 ttgagaaaga taagaaccat ttgcattcat tcccatacct acacccataa agccggtcat
    16501 tgctcctgct tcattttttc cggctgcttc cataccacgt gcaatggaac cttgcacgta
    16561 tccttcacgt acgctaggat cacctagcat cgccccttcg ttacgcatat tgatcagttt
    16621 cacagaatca tcggtgtaag aaatgctggc tacagccaca gaaacaatct ccatcccacg
    16681 taattcacgc caagaatcat ccaatgcagt atccatgtat ttgcttaatt ccaaactctt
    16741 agatggtaca taagaaatcc gttgaccatc tgcagacatc tgattgattg ctgattgtag
    16801 cgctgttagg aattcagcta aatactgctc attgatatca ttgatttcca cttgtgtttt
    16861 gtttttaggg attgcgtttg tataaaataa aatcgggtca acgacatgaa tagaataagt
    16921 gccatgtgcc cgtaagaata attctgcgtt atagaaattg tcaaagtagt tcaacggcgc
    16981 gcttgtaccg aatttgattc cctttatttc ttggagattg atataaaaaa cttgttgttt
    17041 ctgcggtgtg acgccgccaa atttgaatcg gtcaaaagtt tcagcaattg cttctttcag
    17101 tgttccattg aacatggaag gagctgcgtc atttttaatg gtgtaataac cttcctcagc
    17161 ggtataatcg atgatttttc caccatcgac caaaagcatc atcatgtttg gatagacatg
    17221 gacgactgtt ccgtctgtta aaacatcttc tgttccttta cgattggagc cacgtttgtc
    17281 atctttgcgg actttgacac cttttgtcat gaccgttgta tcactcatat tgtctggttc
    17341 gatgacttct agccattgat ctgccagacc gccgccaacc atacttgttg ctgctttgat
    17401 aagacccata aaaaattcct cctctgattc agctagtgcc aaacaattat tgtttattta
    17461 ttataccata ggaagatgtg aaacttctcg gactagagac ggaattgaaa tggaaaattg
    17521 cgtattgttt aaatacctga aaaagccgag aaggatagta gtttttttac ctaatatcac
    17581 gtataataag agatagtgaa ttttttaaaa aagggggatc cttatggcat atcaagcact
    17641 ttatcgagtc tggcgttcgc agcgtttcga agacatcgtt ggacaaaaag ctgtgacaca
    17701 gacactaaaa aatgctattg tttcccacaa aacctcacat gcctacttgt ttacaggacc
    17761 cagaggaacc ggaaagacga gtgcagcaaa gattttcgca aaagcaatca actgtccgaa
    17821 cagtaaagac ggtgagccat gcaacgaatg cgaaatgtgt cgatcaatta ctgctggtac
    17881 gcaagaagat gtgatcgaaa tcgatgctgc cagcaataac ggagtcgaag aaatccgatt
    17941 tatccgtgat cgggcaaatt atgcgcctac aaaggcagcg tacaaagtgt acattattga
    18001 tgaggtacat atgctttcaa caggagcttt caatgccttg ttgaaaacat tggaagagcc
    18061 aaaagaaagt gtcatattca tcttagcaac gaccgaacct cacaaaatac cagctacgat
    18121 catttcacgg actcagcgat ttgattttaa acggatcaat acggcagata ttgtcgaaca
    18181 tcttgaattt atcttgaaag aatcgactat cagctatgaa ccagctgcat tgaatgtgat
    18241 tgcccgttct gcagaaggcg ggatgcggga tgcattgagt attcttgatc aggcaatctc
    18301 gtttagtgaa ggaacaatta ctttagatga tgccatgcaa gtgacaggta gcttaactga
    18361 cgaaatgatg gacagttact tgagtgcttg cgtgaaaaaa gaagtaccag aagcattgga
    18421 aacattgaac caaattctcg cagccggcaa agaatcacgt cgctttctag aggatatgtt
    18481 acaatactgc cgtgacttgc tgatgtatca gcaagcacct aacctagttg aggaacaatc
    18541 gagtacgatc agaagtacat ttacagaatt ggcacaaact gtttcagcgg atcagctgtt
    18601 ttcaatgata aagattttga gcgatactca aaatgaaatt cgttttacca atagtgccgc
    18661 tatttatttg gaagtcgcta cagtcaaact tgcacagcta ccgtctgctt ctgttccttc
    18721 agcagatgta gcatttgcag atgatcagat agtaaaacag ctacaacaag agattaagga
    18781 actaagaaac gaaatcaagg aactacgcag taatacagga attgagaata caaaagtaca
    18841 gccggtacag caaacaaatc ataaaaaaca gacaagtacc tatcgtgtac cgacggaacg
    18901 agtatataaa gttctgcggg aagcgacaag acagcatctg aaccaagtaa aagaagtatg
    18961 ggaagacttg ctgatgagtc tatctgtcac tcagcgagca atgcttaaag caagcgaacc
    19021 agttgcagct ggacctacag gactagtgat tgcgtttgat tatgagattg tttgtcaaag
    19081 agcagcaaat gacgaagaat tgcagctttc ggtacacaac aacttaagcc gaatgatcaa
    19141 agattatgcg cctgatcctg tttatattac aagagaaagc tggccagaac ttcgaagaaa
    19201 ttatttgacg caggcacaag aagagggaac agaaaactct tttgcttctg aagactcaga
    19261 aggagaaatc gagttgcttc ctcaagaaca agaagtacca gaagaaacac ttgtagtaaa
    19321 agcagaggaa ctgtttggct ccgaagctgt tacagtggta gatgattaat aaaaaggagt
    19381 agataattat gatgcgtgga atgggcaata tgcaaggcat gatgaaacaa gtacaaaaaa
    19441 tgcaaaaaga aatggcagaa gcacaagagc tgctaaataa aacagagttt acaggtaaag
    19501 ccaccaatga attggtcaaa gcggttttta caggcgatcg tcgcatgaaa gatatccaaa
    19561 tcaatgaaga agtcgttgat cctgatgata tcgagatgct tcaggatctt gtcgtaatgg
    19621 cagtcaatga tgcgatcgca caaattgatg tggaaactga gaaaaaatta gggaaatacg
    19681 caaaaggttt gccaggattt tagttttaaa ctgaaggagt aatgaatatg caatatccag
    19741 aaccaattgc gaaactgatc gacagctaca tgaaacttcc aggaatcggt cagaaaacag
    19801 cgacaagact cgccttttat acgatcgata tgaaagatga agtagtaaat gaatttgcta
    19861 aatctttgtt aagtgtaaaa cgcgatttgc atttttgtag tatctgcgga aacatcacag
    19921 aagaagatcc ctgcgaaata tgtagaaatc cttcacgtga tcgaagcgtt atcctagtgg
    19981 tcgaagagcc gaaagatgta atggcaatgg aaaaaatgcg agaatatcac ggactttatc
    20041 atgttctcca tggtgtgtta tcaccgatgg aagggacagg tccagaagac atcaatattt
    20101 cttctctgtt gcagcggctg catgatgcag atgtaaaaga agtgattatc gcaacaaatg
    20161 caacaacaga gggagaagca acagctatgt atctctcacg tctgatcaaa cccgcaggta
    20221 tcaaagtgac tcgtctagca catggtctgt ctgtaggctc tgatatcgag tatgcagatg
    20281 aagttacttt attgaaagct gtcgaaggaa gacgtgagtt ataaatttct aattaagtgt
    20341 tttttcagat acaatataaa agcagcacaa gagatggggg caacaatgtg aacggactat
    20401 ttattacaat cgaggggcct gatggtgcag gaaaaaccag catcttgaat gaactttacc
    20461 ctcttttgaa agaagtagca aaaacagaaa tcattcaaac aagagaacca ggaggcattc
    20521 cgattgctga agagatccga gcagtgatcc ttgatccaaa aaatgatcgg atggatgaac
    20581 gtacggaagc actgctttat gcagcagcaa gaagacaaca tctagtcgaa aaggttctgc
    20641 ctgctctggc aaaagggaaa atcgttttat gcgatagatt tgtcgacagt tctcttgctt
    20701 atcaaggtgc aggccgacgc atcggcgtta ccgagattgc tcggttgaat gagtttgcta
    20761 cagaaggtac tacgccggat tttacccttt atcttgacgt agattcagac accggattaa
    20821 gaagaatcaa gaaaaacaga caaaatcaaa tcgaccgttt agattcggaa ggtttggaat
    20881 tccaccagcg tgtacgtcat gcttacttaa agctggcaga agagaatccg gaaagaatcc
    20941 acaaagttga tgcaagaaaa agctttgaag aagtgctgca aacaagttat catacaatta
    21001 ttgaacagta tccgcaattt tttgaaaact aggaaggtga acgtttatga aaattatttt
    21061 agcgattatc caagacaaag acagcaaccg tttatcaaat gaattgattg atgcaaatat
    21121 ccgtgccaca aaactatcat cgacaggcgg cttccttaag gctggaaaca gtacgtttat
    21181 cgtgggtatt gaagatgacc gtgtagaaga agcattggaa atcatcaaaa aaacgtgtga
    21241 atcaagaaag caatttgtat ctacacctgt tacactggat atttcgatgg atgggggcgt
    21301 cccttatcca gtagaagtag aagtaggcgg tgccacagtc ttcgtcttac ctgttgaagg
    21361 attccatcaa ttttaggagg agctgagcta tgaatcaaga aataaacttg gctcaaatgc
    21421 aaccgatcgt gtatcagcag cttcaacgga gttttgagca tgggcgtatc gctcatgctt
    21481 atctttttga aggggagaaa ggaacaggaa aacatgaagt aggtatatgg cttgcacagc
    21541 acctgttctg tacccaaatg aaagacaatc tgccttgtgg aaaatgcaac aattgtcagc
    21601 ggatcgaaaa gaaagaacat ccagatgttt tggttattga accggaagga caaaccatca
    21661 aagtcgatca gatcagacgt ttgcagacgg aattcagtcg cagcggttac gaatcaagaa
    21721 aaaaagtttt tttgatcaaa gaagctgaaa aaatgaatag cagtgcggca aacagtttgt
    21781 tgaaattctt ggaagagccc cctggagatt ttttagcggt gttagaaaca gatgcagtag
    21841 gaagaattct ccctactatt caatctcgtt gtcaagttct gcattttcaa gaactttcga
    21901 aagaagcact gatccatcga ttgcaagaag agcagattcc attggaaaaa gcaaaacttt
    21961 tggctttctt aacaaacagt ttaggaaaag cagttgaaat ctctcaagat gaatggttta
    22021 atgatgctaa ggacttgatt tatcaatggt tcgtttactt gcaaaagcaa gatactcaag
    22081 cctttatcta tgtccaaaaa aaattggtaa aatcatttaa agaaaaaaca caacagttta
    22141 caggattatc tgtcttgatg ttttattatc aggaagcaca gcgtcaagca gtagcaaacg
    22201 gcgaatatgg caaagtgaaa cagataaacc agataattga acggatattg atcgcagagc
    22261 agaaactacg cagcaatgtc agttttcagg ctgttgcgga acagttcgtt ttgcatacga
    22321 ttttttagct tgccgcaaag gatgatgatc aaggaggatg gaaatggtag aagtagtagg
    22381 tgtccgatat agagatgcag gtcatatcta ttattttgca ccaggagact ctgaatatgt
    22441 ttatagtgac aaggtattag tcgaatctca gcaatcctta catattgcta ctgttgtcct
    22501 tcctaaaaaa gagattgatc ccaatgacct tcctgaagct atccgtccga tcttgcataa
    22561 agcgtcacaa gctgaattag aaaaagacaa aaaaaacaaa aaagatgctt atgatgcatt
    22621 gaaaatcgca aaagaaaaag tgcagttgcg tgagttggaa atgaaaatgg tcaaagccga
    22681 atacacattt gatcgaaata aattgatttt ttctttcaca gcggacgggc gaattgactt
    22741 tagagaattg gtaaaagatc tggccgctgt atttcgtact cgtattgagc ttcgacaaat
    22801 tggtgtccgc gatgaagcaa agataatagg cggaattggt ccatgtgggc gtccattatg
    22861 ctgttcttct tttttaggcg atttcgtgcc tgtatcaatc aaaatggcaa aagatcaaag
    22921 tttatctttg aatccaacaa aaatttccgg tttatgcgga cgcttgatgt gctgtctgca
    22981 atatgagagc gaagaatacg agacagcgaa aaaagaactc cctgactttg gaaaagtaat
    23041 tgctacacct gatggtaaag ggaaagtcgt agggttgaat ctgcttagcc gtatcgttag
    23101 tattcggctg actggacggg aaaatcctgt cgaatatgaa tgggatgaat tgaaagaatt
    23161 tatccaagta ggtgagatga atggataaga gatcattata cgacggattg aatcaattag
    23221 aatcagagtt gagaaataca ttaacagaac ttgttgaaat gaagcatgct ttgcatgaga
    23281 tcgtagaaaa aaatacaact ttggagatgg aaaatcaacg tctgcgggaa cacctgcggg
    23341 aattgaacca agttgcacaa gctccagctg aaaatgtgaa gcaggaactt tctaagtctc
    23401 gtatgaatct tgaaaaaatt tatgaagaag gattccatgt ctgctacgat ttatatggat
    23461 caagaaggga caatgacgag ccttgtgcgt tttgtctaga agtgatctat cgcgaaagcg
    23521 gtcgatgacc aataacaaga ggagactgga gcggagaaat actgctccag tctttttatt
    23581 aaggagattg tgacagatgt ataatcaaaa aagtttcaag ggaaatccgg tatatgggga
    23641 tttatatctt gtaccgacgc caattggaaa tttagaagat atgagctaca gaagtgttcg
    23701 tatgctgcaa gaatccgatc ttatcgcaag tgaggatacg cgtaatacac aaaagttatt
    23761 gaatcatttt gaaatccata caccccaaaa aagtttacat gaacataatt ataaagaacg
    23821 tgtccctcaa ttgattgctt tgttaaaaga aggaaaaacg attgctcaag tcagtgacgc
    23881 aggaatgcct tccatcagcg atcctggaca tgagctagtt ttagcctgca taaaagaagg
    23941 gatttctgtg atctctatcc caggacctac agctgggatg acggctttga ttgcttcagg
    24001 gctagtgcct caaccatttt tattttatgg cttccttcca cgtaagaaaa aagaacagaa
    24061 agaaacattg aaaaagctaa aagaacagac agttacgctg attttttatg agtcacctta
    24121 tcggatcact gctacattga cgaatttctt agaaattttt ggaaatcgcc aagtcgtttt
    24181 gtgtcgggaa ttgacaaagg tccatgaaga ataccttaga ggaagtacgg aagagctact
    24241 cgcttatatc gaagaacatc cagtaaaggg tgaatgttgt ctgcttgtag aagggaattt
    24301 gttcgaaatg ccagtgacgg aagttgatga agagttggga acgttaaagg aacaagtaga
    24361 agcaaagatc gcttcaggcg aaaaaccaaa tgctgctatc aaagcagtag cgctgaaaaa
    24421 tgggctgaaa aaacaagaag tctaccgtca gtaccatgaa ttagactgag atagtataag
    24481 ttgtctataa tgaaagacta atgaggaaat cagctactaa cttttcaaat ctcttgaacg
    24541 cgaacgaatg ttcttgtata atgaataaga ggtgaggatg atggagctgc aatttccttt
    24601 acataatgat acatcaagaa aaatcattca tattgatatg gatgcttttt tcgcttcggt
    24661 tgaagaacga gatcatcctg agtttcgcgg acatccatta gtaattgcca gacaccccag
    24721 tgacactggt gggcgtggag tagtgactac agcaaattat gaagcaagaa aattcggaat
    24781 tcattcagca atgagtgcac aaaaagcgta tgagctttgt ccgcaagcca tctttaagcc
    24841 tggcgaccat cataaataca gcgatatttc caaacaagta cgggaagtat tctatcgtta
    24901 tacagatatt gtggaaccag ttagtatcga tgaagcgtat cttgatgtga ccgaaaataa
    24961 aatcaattgc aagtcagcca tcaaaatcgc ccgaatgatc caagcagata tttgggagag
    25021 tgtccagcta acatgttctg cgggagtaag ttacaataag tttttggcca aattagcatc
    25081 agactatcaa aaacctagag gaatcacggt agttgcccct caagacgctg tgccattttt
    25141 aaaagctttg ccaattgaaa aatttcatgg ggtagggaag aaaactgtac ctagaatgca
    25201 tgagctaggg atttatacag gaaaagacct atatgaatgc acagaaatga tgttgattcg
    25261 aaattttgga aaaatgggct attctcttta tcgtaaagtc agaggaatcc atgattctcc
    25321 agtaaacgtt acaagagagc gaaaatctgt gggaaaagag catacttatg ggcagccgtt
    25381 gcaaacggaa gaagcagtgc tgactcagtt gcgccaacta gctgaaaaag tagaagaagc
    25441 actgagaaga gtccaaaaac atgggaaaac agtagttttg aaggtcaggt acacggacta
    25501 ttctactgtg accaaaagag tgacattgcc agaatatatc tataaaaaag aagcactctt
    25561 ttatcaagca agccttatat gggaggaaat cttgggagta gaaaaaggaa tccgattgtt
    25621 agggattact ttgacgaatc tggacccgat gacttatgaa aacatcgtcc ttccattatg
    25681 ggaaaatcaa gaaatataaa aacagctttg agagttgaaa ccggaaatcc gttttctctc
    25741 ccaaagctgt ttttatatca agtcttgctt tttgatttgt tcataggttt ctgttgcatc
    25801 cgtacatttt tcccaaatca caacttgatt ggattctaaa gatttttgga cgtcaatgat
    25861 ccgctggttg ctacttccgc gaaattgtag ttttaggtca cgtttgctta attcaaagcg
    25921 tccgtcaaca aggatatcga tttgcgaaag gagttctaat ttatcttctg aatcttgtag
    25981 caattcatca aaggtatagc ctgtccatga ccagatatct tttgtcgtac cgaactcttt
    26041 tcggatacgt cgtacgactt gcagacaaac atctgtattc aaaaaaggtt cgccaccaag
    26101 taaagttaat ccttgcacgt aatcgttgcg caagtcagcg atgatttgtt cttctaattc
    26161 aggtgtgaat ggtttgccat atcggaaatt ttgtgcagct ttgttgaagc agccttcgca
    26221 ggcaaataag cagccactaa cgtaaagact gttgcggaca ccttcaccat cgacaaaatt
    26281 aaacggtttg tagtcagcaa tatagttttg gctgtagtct tttgctaacc attcttttgg
    26341 ttttggattt ctcataaaaa aacttctttc atttaaggtt caaagatata gctattgaag
    26401 ataaaatcta ctcgaccaca taaataagaa caaactgacc taaatccatt gttcaagaaa
    26461 gcttgttctt attttgatag agggcgttca aagatacaaa gccatcgaaa gagggtggtg
    26521 aaaaaagact tgtcatagcc tcttattctg aataaacggt gttcaaaagc ttgcatctgc
    26581 agtaataagc ccaactaaac gaaaaatatg aggaactatt tttgtttagc tattcttatt
    26641 acttgatgca ggacagcttt ttcacaacct catttcatgt gtttcacacg tgaggaaatt
    26701 tctttatggc ggccatgtac catcggacga gcttgcggat ttcctaaata gccacaagta
    26761 cgtttgacta catcacatgt ttttggatca tgatttccac attctggaca ttcgaaccct
    26821 cgttctgtcg gtttgaaatc accttcgaaa ccacattgat agcaatgatc gattggcgta
    26881 ttcgtaccaa gatacccgat tttgtcatat gcaaaatccc atacagcttc taaagctttc
    26941 ggattttgtt ggagcatagg gtattcacag tagtggatga atccacctga acaatatttt
    27001 ggataatcct tctcaaattc caatttttca aatggcgttg ggtttttacg cacatcatag
    27061 tggaaactgt ttgtataata ttctttatct gtgatatttt cgatgatgcc gaatttttct
    27121 gtatccaaac ggcagaaacg gtcagttaga ctttcacttg gagttgaata gacactgaaa
    27181 tgataaccat attcatcccc ccatgcatct gcattagctt ttaattcttt gacgatatca
    27241 agtgtgaaag cttttgcttc tgggtttgtt tcccattcgc caccgaagaa agctgaagca
    27301 acttcataca atccaatgta gcctaacgat acagtagctc gacggttttt gaataattca
    27361 ttgacagaat cggtacgtga aagacgtttc ccgaaagcac catacatata taaaattggc
    27421 gcatttgctg gaatggcttc tttacaacgt tctacacgat agagaagcgc atctttcatg
    27481 atttctaagc ggtcagacag taactgccag aatttattct gatctccttt tgattcgatc
    27541 gcgattcttg gcaaattcaa tgtaacaaca cctaaattca ttcgtccgac atttacttct
    27601 tgaccatttt cgtctttcca cccttgcaag aatgaacgac agcccatagg tactttgaaa
    27661 cttccagtta attcaacgat tttatcgtaa ttcagaatat caggatacat tcttttcgtt
    27721 gcacattcca atgctagttg tttgatgtcg taattaggat cttcagggtt taagttcaca
    27781 ccttttttca aagtgaagat cagtttaggg aagatagctg tccgcttttc actccctaat
    27841 ccattgattc gaatctgtaa aattgctttt tgaatttctc tttcaaacca gttttctcct
    27901 agtccaaatc ctaatgaagt gaaaggagtt tgtccatttg aagtaaataa ggtattgatt
    27961 tcatattcca aactttgcat agcatcgaag atatcttttt tggtttttgc ttttgcatat
    28021 tctttttggc gttctggcga atcgatccat tcttccgcgt cttttagatg ttttttatag
    28081 ttcaattctg caaaaggagc aagcaattca tctgttcggt cggcagaaca gccaccatat
    28141 tgacttgacg ctacattggc aataatctgt gagatttgtg ctgtggcggt ctggatagat
    28201 tttggcgatt cgacttcggc attgccgatt ttgaacccat tgttcagcat gcctttgaaa
    28261 tcaatcaagc agcaattcgt catcggtgta taaggatgat aatccaaatc atggtaatgg
    28321 atatcgcctt tttgatgcgc atttgctata tgtggtggca acattttcag tccgattgat
    28381 tttccaacga tcccggctgt cagatctcgc tgtgtattga aaacattact gtctttattt
    28441 gcgttttcgt tcacgacagt ttggtccttg ttgattagct tttcaatcga aaagttgata
    28501 tctgttgctt tactgcgagc aaaatctcgt ttggtacgat aattgatgta ctcttctgct
    28561 aattcatatt cgtttttttc tagtaatata tgttccacga tgttttgaat ctcatagatt
    28621 ttgacatttt cagcaaaccg gcttttgatt tcttggacaa ccaagtctgc aatcatttga
    28681 attttttcat aagtaagtgg tgataatgag ccgtgaattt tcttttctgc tttgatcagt
    28741 gcttcattga tcttttgttc atcgaaatca acatggcggc catcgcgttt gatgactttc
    28801 atctgggatg tctctggctg ctgagtaacg acagtttttt cttcataatt tactttcatt
    28861 atcttcaact cctgctaaat tgttcttgtc tatcataaaa caaaatcggg gaaggagcaa
    28921 ctaaatatcg gctatatata gtattcaatg
//
LOCUS       DAHHZJ010000019        28057 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.020, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000019 DAHHZJ010000000
VERSION     DAHHZJ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.020"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(526..1233)
                     /locus_tag="NBL02_000683"
     CDS             complement(526..1233)
                     /locus_tag="NBL02_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571391.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(1298..2134)
                     /locus_tag="NBL02_000684"
     CDS             complement(1298..2134)
                     /locus_tag="NBL02_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCD2571392.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2152..3561)
                     /locus_tag="NBL02_000685"
     CDS             complement(2152..3561)
                     /locus_tag="NBL02_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCD2571393.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3575..5398)
                     /locus_tag="NBL02_000686"
     CDS             complement(3575..5398)
                     /locus_tag="NBL02_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCD2571394.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6241..6567)
                     /locus_tag="NBL02_000687"
     CDS             complement(6241..6567)
                     /locus_tag="NBL02_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571395.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6613..6975)
                     /locus_tag="NBL02_000688"
     CDS             complement(6613..6975)
                     /locus_tag="NBL02_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCD2571396.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(7012..7227)
                     /locus_tag="NBL02_000689"
     CDS             complement(7012..7227)
                     /locus_tag="NBL02_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD2571397.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            7354..7689
                     /locus_tag="NBL02_000690"
     CDS             7354..7689
                     /locus_tag="NBL02_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD2571398.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7765..9762)
                     /locus_tag="NBL02_000691"
     CDS             complement(7765..9762)
                     /locus_tag="NBL02_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571399.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(9860..10135)
                     /locus_tag="NBL02_000692"
     CDS             complement(9860..10135)
                     /locus_tag="NBL02_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD2571400.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10367..11101
                     /locus_tag="NBL02_000693"
     CDS             10367..11101
                     /locus_tag="NBL02_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD2571401.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            11140..12282
                     /locus_tag="NBL02_000694"
     CDS             11140..12282
                     /locus_tag="NBL02_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCD2571402.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(12377..13768)
                     /gene="sufB"
                     /locus_tag="NBL02_000695"
     CDS             complement(12377..13768)
                     /gene="sufB"
                     /locus_tag="NBL02_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCD2571403.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKAVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(13773..14243)
                     /locus_tag="NBL02_000696"
     CDS             complement(13773..14243)
                     /locus_tag="NBL02_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HCD2571404.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(14230..15465)
                     /locus_tag="NBL02_000697"
     CDS             complement(14230..15465)
                     /locus_tag="NBL02_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCD2571405.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(15462..16748)
                     /gene="sufD"
                     /locus_tag="NBL02_000698"
     CDS             complement(15462..16748)
                     /gene="sufD"
                     /locus_tag="NBL02_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCD2571406.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(16761..17534)
                     /gene="sufC"
                     /locus_tag="NBL02_000699"
     CDS             complement(16761..17534)
                     /gene="sufC"
                     /locus_tag="NBL02_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCD2571407.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(17731..18579)
                     /locus_tag="NBL02_000700"
     CDS             complement(17731..18579)
                     /locus_tag="NBL02_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HCD2571408.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(18603..19289)
                     /locus_tag="NBL02_000701"
     CDS             complement(18603..19289)
                     /locus_tag="NBL02_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2571409.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(19282..20316)
                     /locus_tag="NBL02_000702"
     CDS             complement(19282..20316)
                     /locus_tag="NBL02_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD2571410.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(20379..20635)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(20711..21049)
                     /locus_tag="NBL02_000703"
     CDS             complement(20711..21049)
                     /locus_tag="NBL02_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HCD2571411.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(21051..21407)
                     /locus_tag="NBL02_000704"
     CDS             complement(21051..21407)
                     /locus_tag="NBL02_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HCD2571412.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(21413..22600)
                     /locus_tag="NBL02_000705"
     CDS             complement(21413..22600)
                     /locus_tag="NBL02_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HCD2571413.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(22791..23636)
                     /locus_tag="NBL02_000706"
     CDS             complement(22791..23636)
                     /locus_tag="NBL02_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCD2571414.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            23870..24835
                     /locus_tag="NBL02_000707"
     CDS             23870..24835
                     /locus_tag="NBL02_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HCD2571415.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25212..26255
                     /locus_tag="NBL02_000708"
     CDS             25212..26255
                     /locus_tag="NBL02_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HCD2571416.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(26317..26781)
                     /locus_tag="NBL02_000709"
     CDS             complement(26317..26781)
                     /locus_tag="NBL02_000709"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCD2571417.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            27027..27974
                     /locus_tag="NBL02_000710"
     CDS             27027..27974
                     /locus_tag="NBL02_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCD2571418.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
BASE COUNT         8180 a         5828 c         4849 g         9200 t
ORIGIN      
        1 cccttttata aaaagcaaaa aaaaaaagaa agaaacttat tcttgctaat aaactacgcg
       61 aaaggagtct aacgttgttt gatataactt agactgatcg ttaacttatt ttttatattt
      121 ctacatagtt atctttagaa ccatttcaga tacatatgaa acaattacat atgatttttt
      181 cataaaaaaa cagtactgtc tagtagtata aaatatacaa atttcttaaa aattttttca
      241 ctaaaatcac tacctgaaac agtctaaaac acttatatat caacgtttaa tgaaactatc
      301 tccgtttcat ttcagataca aataaatata gtcgggctcc aaatccgaaa aaaaataccc
      361 ccagtagctt atcctaagct actggagatg tataagtacg gtcatataac aacggggtag
      421 ggtaaataaa tgaaaaagat ggaagaatca ttattcaata acttttttaa atacttcgtt
      481 gtaatcagtt acctgtccgt tcttaaatac cacagaataa tctttttacc cattttcaag
      541 taaccgaagt ggaatgtctt taccaacttg ctctgtcact gcattactga tacctgttag
      601 attagctaat aaatctgata aagattgagc atctgtagaa atctctgtat cagtatttat
      661 cgtgattcct ttatcttcat ttacaagctt ccaagtaggt ggtatagctg tgtatgtttc
      721 gatgaatgaa tcaattcttt catctatcgt cttcccagta tctacttctg tctcattact
      781 cttagatgaa tcgattcttt catccatcgt ctgctccgta actgcttctg tctcattatt
      841 ctcaccggaa actctcttca cacccaaact tatatattgt ccatctttag tgtgcatagt
      901 taaaattccc acgcttttac taataaattc actataacca gttctttcaa tagagacttc
      961 aatgtatttc acactatcat actcaaaaga aaaatagaca tgtaaaagca ttttgttgtt
     1021 tacatctaca taataatcct ttgactctac ttgagacctt cctatacgga atacagcttt
     1081 actgtctgat atctctatac cagcatcctt attattaatc tgccaatctc caagtagctc
     1141 ttctggcaag ctagtagttt cttgtgaaca ctttaccact tcactggtag aggtcgaaac
     1201 attcacctcc gcactgttag aagaattttc caaggaatca ggagccttag cacaaccaac
     1261 aagtctttat atattgtgca gccgatattt tcttcattca gtacaccctt gaaatatgca
     1321 gtgttagata aagcagttcg tcctgatttg ctgaggcacc catctcttct tgaacgtact
     1381 ccttgatttt caaggccgcc cgatactctt tcggaaaacg ctgcttcact gaagccagca
     1441 aaaaatcatc cttctccggc aactcttcct tcttaatcat tcgggcaata aaaaactgta
     1501 aatgcgtcac aaatcttaag taaagcatcg aatctttatc aacctttttt tcgggaaaca
     1561 gatatcctag catagccaag atatccttta gtgctcttac cgagagcatg gtttcttcca
     1621 tatttgcttg ttcactttgg gcatttacaa tgtgaaacgc aatgtttcca gcctcctctt
     1681 ctggcaactg aacattcatt tttttgttga tcaattggat agcatagcag ccgatctcaa
     1741 attcttttgg gtaatacttt tgaatttcaa tcagcaggcg attttgaaaa acaatgcctt
     1801 tttgactgcg ttcaatggca tgcttcaagt gatcaaccaa ggtaaaaaat agacttttat
     1861 tcagcttaaa gtcaaacttg gcgttggcat aatcgataat tcgcttgacg caaataatta
     1921 gatcatccgg agcagcctca aacagtttaa tgtaatcctt ttttagagca tcagaaagtg
     1981 aatctaattt aaatactttt tggattttgt cagccggaac caaatcgcct ttctttagtt
     2041 gaaatccaat ccctttcccc atcaaaatta cttctttgcc ttgcttatct aagcaaagaa
     2101 ttaaactgtt gtttaacgtt ttaattactc tcattagatt caccgttctc tctaagctaa
     2161 gccatttctt gcgattactt tttgatacca aaagaagctt tcttttttta ttcgttttaa
     2221 ttctttaaga ttctcatcgg ttcggtcaac atatataaaa ccataacgct tattgaagcc
     2281 ttgatgggta ctaacaacat caatcgctga ccaaggtaaa taaccaaaaa catctacacc
     2341 atctgttaaa gctgcttgaa tagccagaat atgtttggct agataatcaa ttcgatcctg
     2401 gtcattaatt cgtctatctt cctcaactat gtcgggtgcc ccatagccat tttcagtaat
     2461 taaaataggt aaatcatagc gatcgtacaa tttcctcaat gttaatcgta gtccagtagg
     2521 gtcaactacc cagccatatg gcgttttatc tacataagga ttttcagctg cccggtagac
     2581 accttcttct cccaacatga tctgttgatc ccccgctcta gcggaaacat ctgatccgtc
     2641 atttttgctg gcagcgatgg tggcagttga ataataatta atcgcaataa aatcgggttt
     2701 accggaggat aaaattgcct gatcctctgc ctcaatttta ggatataatc cacgatcatt
     2761 caaatagttt tcaaacaaat aattgtattt tccccgaact gctacatcta agaaagacca
     2821 gcctctcaat gtttcccaat tatgggctgc aattgcatct tctggactgg aggtagcttg
     2881 atacatagcc gttaaattga ttgctggacc gatttttcct ttaagattca actgatgata
     2941 caagtgaatt actctagctt gagctaataa aacatggtga ttaatttgaa agagttcttt
     3001 ttttgaaggc agctctccac ttggtggcaa accaatcgct ccaggatgaa ggatcatcgt
     3061 gttttgctca ttaattgtca gccaatagtt caccttgtca ccatactcag taagcaatac
     3121 ctgtacatag tccacaaaat aatcgatagt tgctcgattt aaccaaccgc cctgctcctc
     3181 tagaaaataa ggtaaatcga aatggtacat ggtgacaatc ggttcgattc cctgattcag
     3241 gcattcatca attacttgat gataaaaaga aattcccttt tgattggttt tattattttc
     3301 aatatccata attcgcgtcc aagcgatgga aaagcgaaaa gcttttaagc ctaattcttt
     3361 aaacagctgt atatcttctt tatagtgatg gtaaaaatcc gaggcaactt caaaattagc
     3421 taccccttct gtatgctgat acttgtcaat aatagacggt tgtttcccat cctcctgata
     3481 ggcaccctct gcctggtagg cactaatgga ggcaccccat aaaaacttct ctggaaatgc
     3541 tgaaagctgt ttgtattgca caaaaatcct ctccttaact aatttgcatc aatggttcgt
     3601 gttcctttaa gcttccgctt gcttcactaa ttcctgcgta attttcactg ttggtcacta
     3661 ccacaatcac tgtatcatcg tagccagcgt ttctaatggc agcgaaatcg gcgttgatta
     3721 ataattgtcc tgctgacacc tgatctcctg cttttacaaa tacttcaaag ggttcccctt
     3781 ttaaattcac ggtgtcgatc cccacatgga tcaataattc aataccttct ttagaacgta
     3841 gaccaatcgc atgtttagat tctaaaatag taatgacttc tccctcaaca ggtgaataaa
     3901 tgctgttttc ttctggatga atggctacgc cctttcccaa tagtccctta gaaaagacag
     3961 catcgttcac ttcagaaata ggacacacgg ttccttttac cggattttca acaatgactg
     4021 cttcggctat tgccgtttgc tctgattcat cagaagtatc gtcaaatcca attagataag
     4081 caatgataaa ggttgcaaca aaggcgatta cacacgtaat aattgcgtga acaatattca
     4141 ttggattttc tccaataaag gctggtaagg ctgctaagcc aggggaaaca aaggcatatc
     4201 tgactaaacc gctcaagcca gcatacaaac cagcaattgc accaccaatc atggaagcaa
     4261 ctaatggttt tttcagcttc aaggttactc catagagtga aggctcagta attcccatta
     4321 aagcagtgat cccagtggac acagctagct gcttcaactc tttgttttta gttttcgcgg
     4381 ctacagcaaa ggtagcacct gattgagcaa tattagaagc cagcattcct ggcccattga
     4441 tcatttcaga acccgtactg ctcaattgca tagtagcgat tggcgtcatt gcccaagctg
     4501 tacccgtaac caccaaaaat ggctgcaaac cacccatcaa aaatggaatt aaccaagctg
     4561 ctttgccgtt aatccattca gcccccattg ctataagatc gttcagcaac gttccaaacg
     4621 gtccaatgac aaccaacgct aaaggcccca taattaaaaa taccagcatc ggcttagtaa
     4681 agaatttaat aaacgaaggc gaaattcgat ccgcaaaggg ctctacatag gacatgatcc
     4741 aaacagtaaa gataatcggt aaaactgatc ccgcataatc tgcagctaat accggaattc
     4801 ctagaaaaga aacggcatcg ccagtagaaa aaagctgtga aatatttgga tgaagcaaaa
     4861 tgccagcaac agtaatcgcc aaaattgggt tgacatcaaa ctttatggct gccccatagg
     4921 ctaatagtac tggcataaaa taaaaggctg catcagcaat agtattcaaa atgatgtaag
     4981 tttgactact gtctgatagt acaccagtca aagtcaagac ggccaaaatg gcttttacca
     5041 tcccggcacc tgttatagct ggaatcatcg gtgtaaaggt ggtagaaatg acactaatca
     5101 atcttgaaat aatactttct tgattgtctg aagaggggcc ctttttaatt gaagttccca
     5161 cttccttttg aatttccttg aaggctgttt ggacttcatt gccaatcacc acttggtatt
     5221 gtccaccgct atccaccaca ctaattacac caagtgtttt ttctaaggct ttcgtatcgg
     5281 ctttcctttt atctttgagt tcaaaacgca gtctggtggc acaatgggtt aagctgacca
     5341 cattttcttt tcccccaacc tgttgcaata catttcttcc tacttctctg taatccatta
     5401 ttctctctcc ttaaagcagt aaaaaagacc aagtcaaacc acgccccaat caacattgaa
     5461 gtgttagttt gtcttggtct tgcctgcata atcagtaaca tgccaataac gttattttgc
     5521 tgtaatccaa taatagcaca ttatgatagc gcaatcaaca gggaatttaa ttttttatct
     5581 tttcgtttga ccgtataggg taatataaca gttagatcac atcaactagg gtatatctgc
     5641 gtatacttac tgctaaaata agtatatcta atattaacta taccagtaaa accattccta
     5701 cataaccagc gggaaattcc caaaatcgtg aaagcatcaa aaatgcatat aacggatcac
     5761 acaacttcaa caattctgct cttgggaaaa attgatgaat ttggtgagta aaggatggaa
     5821 taaacagcat tccaacaata taggcaacaa tagtgatact tgtttttttg gtaaaaatca
     5881 gtaagcaata gccaatttca atcattccga gatataaagg tatcgataga atgatttggg
     5941 ctagtattct taaaaattaa ctagcaaatg tttaaactga tttatttcta atctacatgt
     6001 agtaagtatt tcaattccac caattatcaa taaaaatacc aaacatatcc ataataattt
     6061 tttctttttc atttctttta tattgttata acagtgaata acataaaaat tcccgacaat
     6121 cattgtaata tttttttgtc acctgtatta gtatttaatt tataggaaac tataaaaact
     6181 ccctagtgaa tttacacctt tgggagttcc tgtttggacg gtatgcataa tacatttaaa
     6241 tcaatttaac attaacacct cagcatatgg tatctccaca tatatacggt ctttctttgg
     6301 atagtactca atatttatgt tgatagcact tccattgtct aatatagctt tagcccggta
     6361 tgtgttatct ccattttcat gagtaatcgt cacatcatca catgatcgat ttaagttgta
     6421 ctgttcttgt aaaatctgtg ttaccaatgg ttttgatgac ttctctaatc cagatgtacc
     6481 tgtcattttt gaaaaaataa tacctataat agccaagaca acaataccta taatgacaaa
     6541 cttcttgttc atatttactt tattcaactt tttatctcct atctccaaaa taatttgttc
     6601 ttatccctct tatcagtaca ttgacatccc taaaagtaca ccaaagattg ctccagctac
     6661 ccctgctatc atcaaaatcg tacccatttt ctcatctttt tctcgatata agtagcccat
     6721 aataacagca acagcaccaa atacaacagg aataaatgct aacgatacta ccatagaaat
     6781 ccaaccaata atcaaataca gcttccctgt ctcttgtcca tgtacgattt gttccttcat
     6841 ttcattaatt gtctgctcag tacttttctt tactgtgtct gttccggttg tctcatctgt
     6901 ttcttgtagt gctccacaaa actggcaata cttactttca tctgttattt ctttgccaca
     6961 ttttctacaa tacatgtctt ctcctcctaa aatcattcaa ttgcttatta gttaactgtt
     7021 tactttatta aatacatcag aaaacagctc attcattggt acacttaata tctcagaaag
     7081 catcttcatt tgatagggtt taggaatcgt tctccctgtt tgccacgaac ttacacattg
     7141 ctgtgtaaca ttcatttttt ttgctaactc cgtttgagaa agtccttttt cttttctgta
     7201 tcggctgaga ttacttactt ttttcatgat tctcccctcc tgttttatta tatacaacaa
     7261 tatgatgtat ttcaactcat aaaatataaa aaacttcaaa taccattaca aatacaacaa
     7321 cttgttgtac tatactgaga tgggggtgta ttaatgaaac agaaacaata tgcaaaggtc
     7381 attagagaaa agcgaaaagc tctcgggtgg acacaaaaag aattagctga aaaaatcttc
     7441 tctacacaac aggcagtagc aagatgggaa aattctgtaa cagagcctaa cctcgatagc
     7501 ttaacagctc tttctagagc cctaggaacc cctgtaagcc attttctaga caatgttgtc
     7561 gtagattatg aggaagagtt tttggctctc tatcgttctc ttagcactga agatgcagtt
     7621 cgaacaattg actatatgaa gctattaaaa aggcaagaaa atgaacgcaa tcagctattg
     7681 aaagattgaa aaataaataa cccaccgaac tttaacggtg ggtgtattga gatgaaataa
     7741 gattaagagc tatggataac ctgtctagtt taatacaatt gggtcttttc tcttccttac
     7801 tctccgtcca tcatctcttg ttacacttgt aaattgatac agaggaacta tcccagctcc
     7861 tgataatatc aattctttta ttgtttcatc tatccctgcc cctttcgcta aatatattaa
     7921 aaaactacta cttgctttca cattgtatag tttcttaata tccttagaag ctcgttcaag
     7981 agtgaatcct tgtttaacaa agtagcctct gttttttatt aaagcatttt tcacgctgtt
     8041 atccccaaca ttacgtaggc gttcacaaac agtgttaata agttctttct ctgtcattag
     8101 atatgtccct tgccacgata agccctgtaa aacttcatgt gcttcatcta aaatcaattg
     8161 tttttccttg ctgagttctg atcgggaatt ttgaggaaat actcttttag cctctttttc
     8221 gttaaaacat atcttaaccc attcataaga aaaatattga tatgtaaaat gaattttttc
     8281 cagaagtgga aggatatctt cattcaattt ttgaatcaca gaagttgcat taaaaacgtc
     8341 taatcgatag acattgcttc tcttatgatt aaagtttcct aacgctttct cttcctttaa
     8401 cctatttagg tattcgagca tcttcaaagg aacatcttca tcatctaatt tttttatcaa
     8461 tcctataaaa gtcataagtg atagtaactt attacattta tctatgtcat aagttttacc
     8521 atagtaagaa ctcatttttt tagctaattg ttcccccgac aaaatacaag tcacactttc
     8581 cttactatca tcaaaaaaat taacatcaga aagcaagaca tcatagacac gtcgcatttc
     8641 ctctagataa ttccttattg tcctgaaaag tttgtctttc attgattcaa gagtgtcaaa
     8701 aagtactttt gattcctctt tcaattttcg aaaatgtgta ggatacaagt ctaggttctc
     8761 taatagccaa ttaaatgttt ctacccgact gtctttcttt aaaattagca tcacaactcc
     8821 aataaaattt tcactgatat cacaattact ggaatgacaa taaaaaatgg aatgcccagt
     8881 tttactggta aaaatactag cagagggatt atgatcgttg tgaaacagac atcttaagtt
     8941 tttacttgac ttaagtaatt tattcatatc aatggttaac aatctttcat aaatctcatt
     9001 aaaagacatg tcggaggaat caaacaggca gtctttaaac cactgtttca tttcttcttt
     9061 gtcgtcattt tttatcaact taacaaaatc ggtttcattg actagttcat actctctccc
     9121 tgaatcactt tgacaatttt tagaacatgt catggtaaaa tcaatcggaa gctcagtaat
     9181 atctaacatg ttgtcttcat taattattat cactgcatct gacttatatc ctccaaaaaa
     9241 cattctattc gaagcattta cattgtcatc acacccgggg atgttctttt tcaagtattc
     9301 gtaaacagca accattttac catagtcagt aattgcttct tttaatagaa agacaatctt
     9361 gtatttttct agattttcag agtaccgaat acttttttgt atcgcaaacg cagaattatg
     9421 aactaaattg atagataaaa ccttttctat gcttaagtat ctactatcat ctttactcaa
     9481 atctactacc ttccccttct catctttaac agtgttatcg acatccagaa taattactct
     9541 ggtttccaca aaactctcct tgtcattgcc tttattcttt gtcttgcttg gaataaatga
     9601 ttgtgatgaa tttagtatct ctacaaactc atctcttgta acttctctat agttatctaa
     9661 acacctatct cttacatcag ttttttcttt ttttgtaact ggcttctcta ataatttttc
     9721 ttgatctaca taaattggaa tattaatcat tgatgtttcc atttgtgttt cctccttata
     9781 aaattctgtt aaagactaaa acatacagat aataaaaaca aactctttat tactatatgt
     9841 tttagtctta tttgtttact caacctttat accgttccat gaaactatca atatcagatt
     9901 ttttaaatct aatgaccgta tcaattttta taacaggtaa cccgtattga gaaacatatt
     9961 ttctaatcgt tcctactgat acacctagat attcacttgc ctgctgatag tccatatact
    10021 ctagagtgac tccagaaata atgtcttcta gcataggctt tagcactgta attatctctt
    10081 tcacttgttg ttcaacttgt atagaaacaa tgctatcaat gatttgtaga gacatatttc
    10141 cccctccttt cgtgattgct tgaacaaatt ttctaagaat tatcttcgta ttttttgctc
    10201 aaaataaaag tattttgtta ttaaccgatt aatcaaacaa aaaatgatta ccagttcatc
    10261 gacatacaca taatacctcg ataaaaaagg ggtgtcaaca gctttgtaaa atttatttac
    10321 aacacccctt ttttgaggta tactaatttt aggaggtgtg gtcaatatga taaaacgtcc
    10381 accaataaat tatttagaac gaaaaaagat actcggaaca aaaataaaag ctataagaaa
    10441 gagtaaaaaa ttaacacaac cagcatttgg tcttatgatt aacaacggtc aattaattga
    10501 caaaaaaact atctacgaat gggagaaggg aacctattta ccaataccag agcgattatc
    10561 ccgaattgct gatttgggaa atatgagtat agaggagtta gtttgtggca atgtagaaga
    10621 atatattttg ggaattattc tttatagaga tagcattgta ctagatggta taacatttcc
    10681 agataaaaac ctgttccagc atcttcgtca acagttccca ccagtacact ctaatttaga
    10741 tacatggctg gatagatatt ccaaattaga accagagatg caagaattta ttgctaacaa
    10801 gacttgtaat aaagttaaaa atgaaaaaat tagtcttttc aatatcttaa aaatagaaga
    10861 attattcatc aatgctattg tagaagaatt tgacaataac attcttttcc tcactagcag
    10921 tatagaagaa ctactagaaa gaatggtaga tgaatggcta cctattcaat tgaaagacat
    10981 gtcttatccc gaagaagctg tgagagaaat tactgataat ataaacaagc tagaacaaac
    11041 gatttcatca attgggaaaa aatacactaa gaaaaaaatg aagggaggtg ataccatcta
    11101 gtggtaagta ctccttgtaa actaagagga gatgatttaa tggcaaaaat atcaaagtac
    11161 aaaaagaaaa acggtcaagt ggcgtggatg tataaaggac acattgccac cgatccacga
    11221 actggtgaaa aaataaatac tacgagaaga ggttttagta gcaaacaaga agctcaaaaa
    11281 gattatgacg agtacagaca tcaaatatta tacggtgtta aaaagaaagc acctgatatg
    11341 acatttgaag acctgtataa tgaatggata actcatcagc gtaccagtgt taaagcttca
    11401 acaatcgcaa tctcagtaag atacgcaaat aatcaaatac ttccagcatt tggtaagctt
    11461 aaactatcga atatctctgt cccatattgt caaaaagtgg tagatgaatg gcacagtaaa
    11521 tatgaaagtt acgattatat gagaaaacag actgcacaaa ttttacgcta cggggttgca
    11581 atgcaatata ttgacaataa tcctatggaa aaaacacttc ttccacgaaa aaaagagtat
    11641 gagaaaaatc gtaaatttta ctcaaaagag gagcttaata atttactgga tgcttttaaa
    11701 gattttggta acatgaaaca atacgccttc ttcagattat tggcatacac aggtatgaga
    11761 aagagtgaag tactcgcttt acagtggaaa gacatagaca cgtttaataa agaactacat
    11821 gtcaataaaa ctcttgctgt agatgaattt ggtaaagtta taattcaatc tccgaaaaca
    11881 agagcttcta gacgagttat ctctttagat actgagactc tttctatcct caacaactgg
    11941 aaattacagc aaaaagaaga gtatttaaaa cttggataca atactagctc taaagagcaa
    12001 catgttttta caacagttaa aaacactcta tacattccaa acactgttaa tgattggtta
    12061 agatatattt tgaaaaaata taacttgcct cgtattacac ctcatgggtt tagacataca
    12121 catgctagtt tattattaga agcaggagaa tctgtcaagg tagttcaaca acgattaggg
    12181 catgaaaata gtaaagtgac attagacatc tatgcacata ttacaaataa tgcacctaag
    12241 aaaacaggtc aagactttgc tgatatgatg gctcatcagt aaaagagctg gtcaaaatct
    12301 ggtcatttct agtatacagg caaaacaaaa aagctagaac ccttatatat caagggttct
    12361 agctcctata acaggtttac ccaacactgc cttccatctc ataactgatc aatcgattga
    12421 gttcgactgc atattccatt ggaagttctt tggtgaatgg ttcaacaaag cccatgacga
    12481 tcatttcagt tgcttcagat tcggtcaggc ctcggctcat cagatagtag agttgttctt
    12541 ctgagatttt tgagactttg gcttcgtgtt ctaaagagac ttggctgttg tggatctcat
    12601 tgaatgggat cgtatcggat ttcgaccatt cgtccatgat gatcgtgtca cattcgatat
    12661 gagagatcga acccgcactg tttttggcaa aggttacttg tcctcgatag tttacttctc
    12721 cgccgtcttt tgaaattgat ttagagacga tagagctaga tgtatttggt gcattgtgga
    12781 tcatttttgc acccgtatct tggatttggt ttgctcctgc aaaggcgatg gagagcatgg
    12841 tgcctcgtgc accttttcca tctaagtaaa cacttgggta tttcatcgtt gtttttgcac
    12901 ctaggtttcc gtcgatccat tcaacggttg cgccttcgta tgcttttgct cgtttggtta
    12961 ctaagttgta cacattgtct gaccagtttt ggatcgttgt gtaacggcaa tacgcatctt
    13021 ttcttgtaaa gatttctacg atggcggcat gcaggctgtt acttgaataa gtaggtgccg
    13081 tacaaccttc gacatagtgg atgcttgctc cttcgtcaac aatgatcagc gtacgttcga
    13141 attgccccgt gttttccgca ttgatccgga agtacgtttg taatggaaca tcgactttga
    13201 cggcttttgg cacgtaaata aaggtcccac ctgaccatac ggctgaattc agcgctgcta
    13261 atttgttgtc ggttggcggt acaagttttg aaaagtactc tttgaataat tcagggtatt
    13321 ctttcaaagc agagtcagtg tcagtaaaga cgattcctaa cttttcgaat tcatctttca
    13381 tattatggta cacgacttct gattcatatt gtgcggaagc accggctaga taagctcgtt
    13441 cagcttctgg tataccaatc ttttcaaatg tatctttgat tttttctggt acgtcttccc
    13501 agtcacgagc gggtttgtca cttggttttt ggtaatattt gatggcatcg aaatcgatgt
    13561 ctgaaaggtc ggggccccat gtttgcattg gcattttgtt aaatgtttct agagatttca
    13621 gacggaattc aagcatccat tctggttcgc ctttgattct tgacatctca cggatcactt
    13681 cttctgtcaa gccttctcct gtactgaaaa ccggttcgac atcatcgtga aacccgaatt
    13741 tatattcttc taattctggt acgcccatgt tctcactcct cttccttgtg gtaatgatgg
    13801 aaagctgcgg taccatcgtt ttccattgct tttcctagtg ctttccaggc aagtgtcgca
    13861 catttgatcc gagcgggaaa tttagctaca ccaccaagca tagcagcatc gcctaattgg
    13921 tcgggatctt tgacttcttt tccttggaca agctgtagaa agtctacagt taaatgttct
    13981 gcttcttcta ttgattttcc gatgacggcg tctgtcatca tcgaggcact tgccgtactg
    14041 atggagcagc cactgccgtc aaaggcgata tttttgatga ttccgtcttt tacatcgact
    14101 tgcaattcga tcacatctcc acatgtgggg ttgttcaatt cgatcttgct tgtcgagtca
    14161 gtcaagcttc cacgatgatg cggatgggaa gaatgatcca agatcacttg acggtataaa
    14221 ttgtctaatt tagatagtcc catgttggaa aaactccttt gttgccatga ttgcttcaac
    14281 taaacggtcg gcgtcttgcg ttgtgttgta gaaataaaag ctcgcgcgag cagtcgctga
    14341 tacttcaagg tacttcaata gcggttgcgc acaatgatga cctgctctga cggctacccc
    14401 ttccatgtct aaagctgtcg cgacatcatg gggatgtaac ccatccaaat tgaatgcaac
    14461 gactcctgtc cgaaaattcg ggtcttgtgg accataaaca gtcaatccat caattgcttg
    14521 taattttggc aacacataag cggctagttc ttcttcatat cgatggatat tgtccatacc
    14581 gagtttttgc aaataatcga ttgcatggcc taatgcgata gcgccggcga tatttggtgt
    14641 ccctgcttcg aatttccaag gtagttcttt ccatgtactg tcgtataatt cgacaaaatc
    14701 gatcatttcg ccaccaaatt caacaggttc catttcttct aaccattttc gtttaccata
    14761 taaaacacca atacctgttg gtccgcacat tttgtgtccg ctgaaagcaa agaaatcaca
    14821 gtctagtgct tggacatcaa tggccatgtg cggggcagat tgagcaccat ctactacgat
    14881 gaccccgccg ttttcatgga caagttcagc taattcttga acaggattga agacacctaa
    14941 gacattggat acatgagcaa tggagacgat tttggttttg tccgtaatct ttttcgctgc
    15001 atcctccata tccaggaagc cttcttctgt tagttcgata tatttcaaga cagcacctgt
    15061 tcgttttgcc aactgctgcc acgggataac attagaatga tgttccatgt aagagagcac
    15121 tatctcgtct cctgctttga tatagcggtc gccgaaggtt ctggctaccc aattcaagct
    15181 tgtcgtcgta ccacgagtaa acagcacttc tgctgtttct tttgcatgga tgaacgcccg
    15241 tactttttct cttgcttgtt cataatcatg ggtcgcgcgt tctgctaacg tatggacacc
    15301 gcgatgaaca ttggcgttat cgtgttcgta atagtacgct aatgcctgga gtacttgttt
    15361 tggcttttgg gtcgtcgctg cattatccag ataaactaaa ggttcatcat tgacgtcttg
    15421 aaataagata ggaaaatcat tgcgtagctt tttagcatcg atcatccatt caacttccct
    15481 tcgattactt cgactaattc tttccgaact tgttctacag ggattgctgt taagacagaa
    15541 ccgaggaatc ctcgaatcac taagcgctct gcttcttctt tatgcaatcc tctactcatc
    15601 agatagtaca tttcttctgg atcaactcgt cctacactgg cagcatgtcc agcagttact
    15661 tcgttttcat cgatcaaaag aataggattc gcatcaccac gtgctttatc agaaagcatc
    15721 aagacacgac tttcttgttg ggcatctgca ccttttgccc ctttcaagat gtggccgata
    15781 ccattaaagg tcaatgttcc tttttctcgg atcactccgt gttgcaaaat atgcccgatc
    15841 gtatgagggg ctttattggt tactcgtgta tcgattcctt ggatttgtct gcccgagctg
    15901 attgccacga ttttgacttc tgcgtgtgca ccttctcctg ctaaatctga gtcaaagtca
    15961 gcgatcacat gaccgtcatt catgacacca attgcccagt cgacagaagc atctcgtaaa
    16021 atgtgcccac ggcgattcat ataagaagta atattttctc ctaattgatc tacggctgaa
    16081 tatttgattt ttgaaccagc tttagcgatg acttccacga tgatattacc tgatgatttt
    16141 gctacttgtt ctttggttgt ctggaaacgt tccagataag aaaattcact atgttcatct
    16201 gccacgatca acacatgctt gaagaagtgc tcatcggatg cgccatcttg aatgaataag
    16261 gactcgagtg gttcatcgat cacgacattt ttaggcacat acaggaaaat accgctgttc
    16321 ataaaagcta catggagtgc agtcaattgg tcttcgttca tgtttactgc ttttttcata
    16381 taatattctt ggactagttc tggatattcg atcattgcag tgaacaagtc ggtaaaaatg
    16441 actccttttt ctgctaattc cacaggcagt tgttcaaaaa tcgtccatga accttgttgg
    16501 acaagcacag gattgtcttt cattgcatca aatgccggaa tcgtgcctgt ttcactgatg
    16561 gtttgtgttt ctttcacatc aaataacgac cagcgatgga atttcacccg atcgatatgc
    16621 ggcaatggca gttcatctgc tttcgctagt gcgtcttgac ggaaggttct catccaatca
    16681 ggttcgcctt tacttaaaga aaaatcattt accgcgtcaa gataatcgag ccagtttttt
    16741 tctttcattc ttgctccccc ttacgcttct tcttctttat agtcgattcc taattcttta
    16801 ctgataccag cgtagccttc tgcttccaga cgtttagcta ggtcagcatt ccctgtcatg
    16861 actactcggc cttccatcat gatgtgaacg acatcaggtg tgatatagtt caataatcgt
    16921 tgatagtggg tgatgatcaa tgcaccgaaa ttgtctccgc gcatttcatt tactccttta
    16981 gcaacgactt tcaaagcatc gatatccaga cctgaatcga tttcgtctaa aattgcaaat
    17041 gtaggttcta acatcaacaa ttgcaggatc tcattccgtt tcttttctcc gccagaaaaa
    17101 ccttcgttca gataacgttc agccatttct tctggcatat ttagtaaagc cattttttca
    17161 tctaactttt tcaaaaatgc cataacagaa attttgtcgt tttcatcacg tttcgcattg
    17221 atcgctgcac gcatgaattc cgcattcgtg atacctggta tctcacttgg atattgcata
    17281 gctaaaaata agccaagtcg cgcacgttca tctacttcca agtctaaaac atttttcccg
    17341 tcaaacaaaa tttcgccttc tgttacttca tagtttggat tgcccatgat agcggcagat
    17401 aaggtagatt ttcctgtgcc gttaggtccc atgatggcat ggatttctcc ggttttcatt
    17461 gttaggttta cgcctttaag gatttctttt tcctcgatcg acacatgtag attttttatt
    17521 tctaagacag acatgcatta tacccccaaa ttctctttcg ttctcactca ttttagacta
    17581 attgagaatg aagcgcaatg accttcctta ctaaaatcaa atatctttta tttattatta
    17641 aacaattttg atttctaaat tagaataggt ataaaaaaga tgagaaataa gccattacag
    17701 acttatttct catctttttt gttccatacc ttattttgca tcagccttta ctggtttaac
    17761 agagccgccc catttgtctg tgatccattc ttggacttct ttgcggtgaa gcacttcaat
    17821 cagttttttg attttgtcac tgtcttcatc acctttacga gtcgcaataa tattcacgta
    17881 aggagagttg tccttttcaa gcaatacggc atcttctaat ggattcaacc ctgcgccgta
    17941 agcgaagttt gcgttgattg caactaggtc accttcatca ttttggtacg tagaagctaa
    18001 aagtgatggg tcgatcgtat ggttgaactt caagttctta gggttttctt caatatcatc
    18061 gaatgttgct gtttccagat ctactccatc tttgactttg actaagccag catcttgtag
    18121 aattgtgatg atgcgtcccc agtctgattc agaatttgaa gtaatgattg ttgcaccatc
    18181 ttttaattcg gaaacatctt tgactttttt agagtacaag cccattggtt cgatgtggac
    18241 agcaccggca ttgacaaagt catatccttt ttctttgatt tctttgttca aataaggaat
    18301 atgctggaag taattggcat caatgtcgcc ttcttccaaa gattggttcg gtaatacata
    18361 gtcatcgaat ttgacgattt caagatcgat tccttcatct ttcaataatg gtttcacatg
    18421 ttccaaaatc tctgcgtgcg gtgtcggtga agcgccgact ttcaatgtcg tgtcgtctgc
    18481 tttatttgct gaatctttgc tgtcgctgtt gttaccgcat gctgctaatc ctacagtcag
    18541 caaaagcgct gctgcaaatc caaaaatttt ctttttcata tcctacatcc ccctatatcc
    18601 tattatcttt tgtctgtttt cttagttaag aaatcaccga ttccttgaac gataaatacg
    18661 attagtaaaa taattaaagt cgcaacgaat gtcactgtta gattaccacg ctggtagcct
    18721 tcctgccaag caagacctcc aagtccacct gcgccgatcg cgccggccat cgcagtaaat
    18781 ccgatcatcg agatcgttgt aactgttaat ccagaaatca atgcgggaat actttcaggt
    18841 atcaagacct tccagatgat ttcccagtaa cttgctccca ttgcatctgc tgcttccagt
    18901 acaccagggc tgacttcacg aaaagcaatc tctactagac gggcataaaa tggtgctgcg
    18961 gataaaacaa gagctggaat ggctgcttgc gcacctagca tagtgcctac gattgcttta
    19021 gtaaatggaa tgatcaaaac catcaggatc ataaatggcg ttgaccggaa aatattactg
    19081 atgatcgata cgattccata aatgattcga gcatacggtt tgtttttgcg tccaatagag
    19141 taaaggatca atcccaaaac taatccgatg atcgtcacta agaccattga gattaatgtc
    19201 atatatagtg tatcaaggat agcctgattc atcgtttccg ggttgatttg tttaaaatct
    19261 agatatgttt ctgaaaagct tttatccacg atggatcacc tcaattccta ctccttgttc
    19321 tttgatgaac acttctgctt gatctaattc tttggtttca ccagtcatca agacagtcaa
    19381 tgaaccaaag gaaccttctt tcacttgttt gatctttcca tacacaacgt tgacatcgat
    19441 gttatagcgt cgaatcgctt gggaaatgac tggctcgttg gcatttcttt cgctaaaggt
    19501 caaagttgcc aaacggccgc ttggattttc tttgatgagt tcagctaata gttctgtcga
    19561 atcagcttct ggttcgatgt cttgttgtac aaagcgtttg gttacttctt ttttcggttg
    19621 tctgaagaca gtcaatacgt ctccttcttc tacgactttc cccaactcca tcacagcgac
    19681 tttgttgcag attttgcgga tcacattcat ttcatgtgtg atcaagacga tggtcaaatt
    19741 caatttctta ttgatcgcta acaataagtc taatacttct tctgttgttt gcgggtccaa
    19801 tgcgctagta gcctcatcac aaagcaggat ctcaggatca tttgccaatg ctcgtgcgat
    19861 accgacgcgt tgtttctgtc cgccggaaag ttgtgaagga tatgcttgtc cgcgtccttc
    19921 taacccgact aaacgcaaca gttcttctgc ccgttcttta cgtttgctct ttggtacgcc
    19981 agctaattcc agcggcagct ggatattttc aagtacagtt cttgaccata acaggttgaa
    20041 atgctggaag atcataccga ttttctttcg gaatgtacgt aattctttgt tttttaattt
    20101 tgtaatatct tgatcgttta cgatcacttc gccgctcgtg ggaagctcaa gtccattcag
    20161 gagacggact aatgtacttt ttcctgcgcc agaatagccg acgattccat aaatatcccc
    20221 tttttcaacg tttagtgaaa cgtcattgac ggcttggatg tttccgtttt tcccattgaa
    20281 tgtctttttg acattcgtta gttggattaa tgacatgcca ttcacatctt tctcttatga
    20341 tttttcaaac aaaaaagcct tcgccctctt gccttaagca aaaggacgaa agcttgaact
    20401 tccgtgttac caccttgatt taccgttact tcgcaataac agcctcttcc agtacttgga
    20461 cttagcttct ttccgtatac tgtcgcgctg taacgggcac tcccgtagta agtttgctta
    20521 cgctcgctta ctctgctcta agaccatctt ccatctcatt ttcgttaccc attttcagca
    20581 aacagggctc tcttcaaacg tccgtgacat gtactcttct tttcaaagct ttctcgtttc
    20641 atataggatt gcttatgatt atagcaactg gaagaatcag tgtcaacttt tttcaagtga
    20701 ttttctgatt ttataattcg tcaaattctt ttggatcaac atctttgaaa cttaagatcc
    20761 acgcattcat ttcatctgaa tcattcaaga tattgatatc ttcaccgatt ttatcattaa
    20821 cagaagaaac tgtacccgtt agcgggctgg caaattcatt gaccgctttt tccgcttcaa
    20881 cttcgatcag aggttcatct ttttttagcg attgtcctgc ttttggcaga ttggcaaacg
    20941 tgatgtctcc aagttcttct tgcgcttcat ttgtcaaacc aacacagtat tcttttccat
    21001 taaataagat ccaaaggttg tcctttttct tcaaatgctt gcctgccatt ttatatcagt
    21061 tctccttcat aaattttttc attgaatcca attgcaacaa gctggtcctc tcgtaagatc
    21121 agtggtcgct tgatcagcat accatctgtc gataagactt ggctcgcttt tgaagccgta
    21181 aactcgccta cttgatcttt cagtccaagt tcccgatatt tcatcccgct tgtgttgaac
    21241 aggcgacgga ttggtacttg actcgtttct agccaagtct caatcgttga ggccttcggt
    21301 acttctttga tcatatcaac ggtttgataa ctaacgccat gctggtctaa ccacgctttg
    21361 gcttttttgc atgttgaaca tttcgggtac cagtaaaagg tgtacaattt tcttacctcc
    21421 ttgaagatct tgcaggtgct tcattttggt atctcatcga taatatcaac ccgataccga
    21481 tcatgttccc taaaatcgag gatcctccct gactaatgaa aggcaatggg atccctgtta
    21541 aaggaagtaa tccgatattt gctccaatat tctcaaagac atggaacagc agcatcatga
    21601 tcaaaccgct tgcgatatag gcgtaaaatt cattattcgt atcgaaacac acccgaatca
    21661 tacgatagat taaaataaaa tacaagaaaa tgacgaatgc accaccgata aagccgaagt
    21721 tttcaccgat aacggtaaaa atcatatcgg actcacgaac aggcacatag atattgctta
    21781 cgttgaatcc tttaccaaac aagccaccgg taccaattgc caaaagtcct tgggccggct
    21841 gataactcat tcctgacgta tcatggaaag ggtccagcca tgaatcaata cgtgcaaact
    21901 gataagattt gaatccaact ttataaagca agtctcttcc gccttcagtc gtaaccaaga
    21961 agatcgttcc tgctccaacg actactgcca agattactac aggtgcgatg atgcgccaag
    22021 agattccaga catcaggaaa atcccgccaa agatcgcaag aaagacaagc atcgttccaa
    22081 agtcatcttg ggccatgatc aatgctaaaa ccggtattgt cacgatcaag agcttcccaa
    22141 tcagccaaaa gtccgatttc atcgtccgtt cttttacttg cgtattatgt ttagttacga
    22201 tcagtgccat catcaagatg tacgcaatct tcatcaactc tgctggctgg aaagtaaacg
    22261 caccgaatcg aaaccagttt ttagaccctg ttgaagcagc taaattctga tcatagaatt
    22321 tgagcaagag tcccattacc actaacccca aaccataaag gtaaggagtc agtttccaga
    22381 tccatttgga attcaaatgc atgatgacaa cgattgctgc cgctccaact aaataccaga
    22441 caacttgcat ccctacgcct ttcaagactg ctggattgtt cggatcatgg ctaagtgcta
    22501 catataatga gagcagacca atcaaacata acagaaagac tggtaaaatc acgccataat
    22561 caatacgatt atcgttcgaa gcttttaatt ttttttccat gtttcatccc ttttcttaaa
    22621 aaccatactg ctgattatta tagcggtcca gttagataat ggaaagtctt tccatagaca
    22681 tttgggaaaa attaactttc cgtttgtgca gttgcttgag caaatgcagt acaaaagcct
    22741 actgcttatc tttagtgtat catagaactt tttttgatac atgcaattat ctaccagctg
    22801 agattctcct cgctgtgaga agtttctgtg ctttcattgt atcgaaggat ctcttcggca
    22861 atctctagca gctgatcaga aaccagattg gttcgttgga tgaaataaaa atatcgatgg
    22921 aaattcaacg gagtcattgg cacattttct ggaacagagc gcttagacaa aatcgagcga
    22981 ccgattcctt ccttcagcaa cgcaacaatt acatcgttac ttttcacgat catcttctta
    23041 tcttggatat tcatctcttc aaaataacgc tgcgtgtaat gaaaaacacc agagtttgct
    23101 tctcttacaa gccacaattt gctttttggg tccccagcga tgactagctg gtcttccatc
    23161 aaagagtatc tttgtacgcc ggaagtctct aatggctttt cgataaaacc aaaatcggat
    23221 tcatgtttcg ctacttgttc taatgattca tgagaattcg tgatttcgat ctcaaaatgc
    23281 actttaggaa accttttgat cagatgtttc atcaactgag gcaaaagata gacagcaaag
    23341 gtgtgggaag caacgatccg acatacttct tcttcatttt gttcattgag caatttttgc
    23401 tgagtttctt cccattcttc taacaaaacc agtgtccgtt catataataa gtcggcttgc
    23461 ttagttggaa gcatctcttt tcttccgtta cgttgaaaaa gatcgatatt caattcccgc
    23521 tccaactgtt tgatttgatt cgagactgct ggttgagata aaaacagtac ttctgctgct
    23581 ctggaaaaat ttcttgtttc atatactgct cgaaaagtcg ttaataactt aaacatggca
    23641 ccacttccat aactttttat gataattact ataaaaacta tttgatttaa atatacaaaa
    23701 aaccatctat aatagaaatg tacaacaaaa cagttgctga tccaactagt aacttagatg
    23761 aagacaatca gctgtctgct tgctcgtaat catacattcc tattcatttt acaatgtatt
    23821 cgttcatctt ggtactaaaa tctattaaat aaaaaggaga atattatata tgaagaactt
    23881 tttgaaaatt attccgatcc cgatctgcgg gttgatcctt gggttagctt cccttggaaa
    23941 tctgtttaaa gactatcacc atgttgcttt aggcaatcta actggcggta tctctatgat
    24001 tcttatgata ttcatttttg ttaaattgtt tatcttattc gaacacacga aacaaacgtt
    24061 gcatgatcca atcatcgcct ccgtttcacc aacttttacg atgtcactca tggttatttg
    24121 tacttatttt gtttcatatg aaagtattgc accaattgtc aaattcattt ggctgtgcgc
    24181 tgttatcttc caagtgatcc ttgtctttta tttcaattat caccatgtta tcaaagctga
    24241 tctttcaatt gaagcaattt atccaagctg gtttatcatt tacgttggat ttggggtcat
    24301 cactgtaact gccgggaact tcttcccact tattggaaaa attttctttt ggatttcatt
    24361 gatctgctat gcgttcttat tgccgatcat catcaaacgt gtctttttcg taagaaatat
    24421 ggctcatccg acacgtccgc tgattacgat cgttgcagca cctggttctc tttgcctaac
    24481 aggttatctt aaaaactttg ctgaacctaa tttttatctt gtcgtagcat tgttcgcttt
    24541 atctcaagta ttgtatttcg ttgtgctgtc tatgcttcca cgtttgttca agcttcgttt
    24601 ttatccaagc tatgcagcct ttacattccc gcttgttatc tctgcaacag ctatatttac
    24661 tactgttcat tacttcaaca gtttaggtat ctatagtagc gtattggatt tcttaaaagt
    24721 tgcggaatgt ctgtttgcaa gtgtagttgt cttctacgtt cttgctcatt acgtacgttt
    24781 cttaattaaa gaacatagca aacatggaaa atttgtttct aaaaaaatcg cttaataatc
    24841 gattcaatag gttgtgaaaa agctgttctg catcaagtaa taagaacggc caaacaaaaa
    24901 cagctgttca tgtttttgta ctggttgggc ttattaccgc agatgcaagc ttttgaacac
    24961 cgtttaccaa gaggtcgtaa aagaagtggt cagcttcaag aaataagaag gaatttacga
    25021 aaattgcttc tcaaattttt gtgaaatttc ggcttatttc cgtagaagct acttctggaa
    25081 caccgtttat tcgaaacaag aggtcgtaca agacttttgt cacgacctct tgttcttttg
    25141 tcaaaatact ggtttagtgc tactatgttc gagtatgctt ttagtattta ttgacgaaag
    25201 gaaccgatca aatgaaatca ttgtcacatc caataaaatc tgtaaaggat tcagcggtcc
    25261 gcgaaatctt acccggttta ctgctttctt ttgccatcgc tgccttcagc aggcttcttg
    25321 ctatttatct gccacagtta ggtggagcga ctattgctat tttattgggt attttattag
    25381 gaaatacttt ttttaaacag cctgttctag caatcggcac aaaatttgct gaaagcaaac
    25441 tattagaatt ctctgttttt ttactaggtg caactgtcac agctcaaacg attgctcaaa
    25501 ttggtgtaaa aggaattgtt tttgtgttac tgcaaatgtc agtcactatc atcggtgctt
    25561 acttgattgg taaaaaactg caattcagcg atacttcttc tttgatgatg gctggaggaa
    25621 acgctgtttg tggttcttct gctattgctt caatcgcccc ttccatccat gcgaaagagg
    25681 aagaaaaagg acagatcatc acactagtca atctactggg tactgtgatg atgttgactc
    25741 tcccttttct tggattacag ttgtttggtg gaaatctcat tgaaaaaagt gcacttttgg
    25801 gtggtacact ccaatctgtt ggtcaagtag tcgctggggc aagcttactt gacaattctg
    25861 ttgtcgaatt cgccatgcta tttaagattc tacgtattat gatgctcgtt gttgttgtct
    25921 atctgtttga aaaattcatg cacaagaaac aaccagctct gtttgaaacg ccaactgata
    25981 cgcagaaaaa acgaaaatta ccgcttcctt ggtatgtatt agggtttgtg atcttttgtt
    26041 tggtcaacag cttgttttct ttacctgtag ttattgacca atcggcacat tttatcagca
    26101 gctggtttga gatcactgct ttagcggcaa ttggtctgcg tttagacttt gcaaaattca
    26161 tgaaagaagg tacacgcttc ttatcttatg gattagctgt cgggcttctc caaacagtga
    26221 tggctttact atttatttgg ctgatcaaag tttagaatga acatgaaaaa acagttgtga
    26281 ttctcgcatg gaacaatgtc cgaatcacaa ctgtttttaa ttttgcagat atccttctaa
    26341 caacaacaat tggcgtttga tttcaacccc atctattgcg tttcccatat atcctgtcag
    26401 ttttccattt tgcccgatca ctcgatgaca aggaataacg attggcaatg gattggttcg
    26461 atttgcctgc ccaatcgctc gcacagcttt cggactatca acagcaacgg ctacttcttt
    26521 atacgttcgg atttctccat aaggaatggt cgacaaagct ttccagacat tttgctgaaa
    26581 aggcgtacct ctctttatag ataaaggaac atcaaattct tttctttttc ccgcaaaata
    26641 ttcttcaaac tgttcttgtg tgcgcgttaa aattcgctga ttcgctgatt cgctttctga
    26701 aattggctga aaagatacag cagtcaatcc ggattcatct gcatctaacc ataaaaagcc
    26761 gataggtgtg tgtattttca ttttgtttgc tcctagtcaa tagactggaa cttcctcctt
    26821 tttctctatc caaaactact tgccaactaa taaaaattgt tcttaaaagt ttgataaacc
    26881 gaggaaacat ggtaaactaa atacgttagt ttacaaaaag aatacctgtt cttttacgta
    26941 aaatcaaatt ttaaaggtgt tatttaaggt ttattaaatt ttgaatgtaa accatatata
    27001 aacatacgat tgaaaggata gaaacgatga aagtatcaat tgttgtccca tgttttaatg
    27061 aagaaaaaac tgttcctctt ttttttgatg aggttgaaaa attcaaaggt gcccatgagt
    27121 tcgaatattt gtttattgac gacggatcaa aagacggtac gttagcagca atcaagaagc
    27181 tagcctcagc ttatccttat gtccgctttg tctcattttc cagaaatttt gggaaagaag
    27241 cggctctgta cgctggattg caagcttcta caggagattt agtgaccgtg atggatgttg
    27301 atctgcaaga tccgcccgaa ctgcttcctg agatgatcaa ccggatcact acttctgata
    27361 tcgattgtgt cggcacccgg cgttcgactc gtgaaggcga gcctgcgata cgcagtttct
    27421 ttgcgaaaaa attctaccag ttgatcaata aaatcagcga tacagaaatg gtcgatggcg
    27481 ctcgtgattt ccgcttgatg actcgtcaaa tggtggatgc cgtcctagaa ttaacagaat
    27541 acaaccgatt ttcaaaagga ttatttagct gggtcggatt taaaacagaa tatatttctt
    27601 atgaaaaccg cgaacgtgtg gctggcgaaa catcttggtc cttctggaaa ttattcaact
    27661 actcgattga tggtatcgtg aacttctcgg atgcaccgtt aacgattgct tcatttatcg
    27721 gcgctttgtc ttgtgtcggt tctggtattg caattttgtt cattattttg cgggcattgt
    27781 tgtttggtga tcctacatca ggatggcctt ccatggtctc gatcttcctg ttcattggtg
    27841 gaatccaatt gttatgtctt ggaatcatcg ggaaatatat tgggaaaatc ttcttagaaa
    27901 cgaaacagcg tcctgtttat atcataagag aaacagaaaa agtgaagcca aatgaattga
    27961 agcctcacca ataatatacg tcaaatgaag taaacatttt tttaaatgaa aaaggactgt
    28021 gacaaaagtc ttgtcacagt ccttttttcc gaataaa
//
LOCUS       DAHHZJ010000020        27497 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.021, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000020 DAHHZJ010000000
VERSION     DAHHZJ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.021"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            6..245
                     /locus_tag="NBL02_000711"
     CDS             6..245
                     /locus_tag="NBL02_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571419.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            312..521
                     /locus_tag="NBL02_000712"
     CDS             312..521
                     /locus_tag="NBL02_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571420.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            581..1252
                     /locus_tag="NBL02_000713"
     CDS             581..1252
                     /locus_tag="NBL02_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HCD2571421.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1305..3281
                     /locus_tag="NBL02_000714"
     CDS             1305..3281
                     /locus_tag="NBL02_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HCD2571422.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            3294..4049
                     /locus_tag="NBL02_000715"
     CDS             3294..4049
                     /locus_tag="NBL02_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCD2571423.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            4046..4870
                     /locus_tag="NBL02_000716"
     CDS             4046..4870
                     /locus_tag="NBL02_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571424.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            5171..7270
                     /locus_tag="NBL02_000717"
     CDS             5171..7270
                     /locus_tag="NBL02_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HCD2571425.1"
                     /translation="MENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSFLANA
                     ANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQDPVED
                     RLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREIQLIF
                     TAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLTVIGS
                     ILFLTLASLGYVVLKLK"
     gene            complement(7308..9365)
                     /locus_tag="NBL02_000718"
     CDS             complement(7308..9365)
                     /locus_tag="NBL02_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HCD2571426.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYMVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVDILKEQIINVTHGESVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFSTPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(9479..9691)
                     /locus_tag="NBL02_000719"
     CDS             complement(9479..9691)
                     /locus_tag="NBL02_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571427.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            9878..10345
                     /locus_tag="NBL02_000720"
     CDS             9878..10345
                     /locus_tag="NBL02_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571428.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            10342..10584
                     /locus_tag="NBL02_000721"
     CDS             10342..10584
                     /locus_tag="NBL02_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HCD2571429.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            10602..10904
                     /locus_tag="NBL02_000722"
     CDS             10602..10904
                     /locus_tag="NBL02_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571430.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            10976..13030
                     /locus_tag="NBL02_000723"
     CDS             10976..13030
                     /locus_tag="NBL02_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571431.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELGQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            13030..15642
                     /locus_tag="NBL02_000724"
     CDS             13030..15642
                     /locus_tag="NBL02_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HCD2571432.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            15639..18170
                     /locus_tag="NBL02_000725"
     CDS             15639..18170
                     /locus_tag="NBL02_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571433.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PEQAKGMAGNDKSGLSDIQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            18222..18554
                     /locus_tag="NBL02_000726"
     CDS             18222..18554
                     /locus_tag="NBL02_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571434.1"
                     /translation="MDKYSNMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            18555..19148
                     /locus_tag="NBL02_000727"
     CDS             18555..19148
                     /locus_tag="NBL02_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571435.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            19311..21005
                     /locus_tag="NBL02_000728"
     CDS             19311..21005
                     /locus_tag="NBL02_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCD2571436.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEIVKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            21693..23570
                     /gene="ltrA"
                     /locus_tag="NBL02_000729"
     CDS             21693..23570
                     /gene="ltrA"
                     /locus_tag="NBL02_000729"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCD2571437.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            23701..24078
                     /locus_tag="NBL02_000730"
                     /pseudo
     CDS             23701..24078
                     /locus_tag="NBL02_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            24078..25283
                     /locus_tag="NBL02_000731"
     CDS             24078..25283
                     /locus_tag="NBL02_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HCD2571438.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            25299..25943
                     /locus_tag="NBL02_000732"
     CDS             25299..25943
                     /locus_tag="NBL02_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571439.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            25954..26760
                     /locus_tag="NBL02_000733"
     CDS             25954..26760
                     /locus_tag="NBL02_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571440.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            26782..27012
                     /locus_tag="NBL02_000734"
     CDS             26782..27012
                     /locus_tag="NBL02_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571441.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
BASE COUNT         9960 a         4303 c         5628 g         7606 t
ORIGIN      
        1 agtagatggc gcgaccagtt ttagaggcga tggatatgga tactttgaca aaaggaaggt
       61 atgtacagct gttagctcgt ggttattcac ctaaagaagt attcaaagcc ttatctaaag
      121 gaacagatat ggaaaaagaa agattgcgaa aagaattcga ttattggaga acccataatg
      181 gaatcaagga tttgaaacca gcacggccag taattaaacg aaagaaaaag ataacccaag
      241 actgaagtgt agagcgtatg tgatcatagg cttttttatt tttgcaaaaa acaaggaagg
      301 taggaaaact catgaattat gaagaaatcg aaaacagaaa aaaagtgtcg aaagaaatgg
      361 aagaaaagtt actaaaaacg atgaagcaaa aacaccttaa aagattatct gtcgcgcaat
      421 atatcaatga tatgcagtta acaggaaaag agaaagcttg tttgttaggt tcaatgaaaa
      481 actttgaaca gttgagacga acttatagaa ttcattttta aaactttttc tatggaaatg
      541 agaaaaagtt tttttgtttt gtctgaaagg aggcagatgg atgaaaaaat ggttatttgg
      601 ttttttagga gtcactttaa tcgtcttttg cagtgtgttt ggctatgtta gctaccaaaa
      661 acatgaagga gaggtgttta agcaaaatat agaaaaaaag atgcccgtag atcaaatcaa
      721 tgcacatgca aagagttata aggaggatgc aacgaacgtc aataatgata tgtctttggg
      781 gcaaatgttg tccatacaaa aagaagcaat cgaaatgggc gtcaataaac aagtatttgc
      841 acaaattcag attcctgcac ttggtttagc attgccaatt tttaaagggg caaatcaata
      901 cactttgagt cttggagcgg ctacttattt ttatgaagat gccgaaatgg gcaaaggaaa
      961 ctatgtccta gcaggtcata acatggaaat gccaggtgtt ttattttctg atattcaaaa
     1021 gttgtcatta ggggaggtga tggacttagt tagtaacgat ggcgtctatc gttacaaggt
     1081 aacgcgtaaa tttatcgtac ctgggtactt taaattaatc gatggcgttc ctgaagagaa
     1141 ctctttcttg tctttgccaa agaaaggaga gaaaccctta ttaacgttgt ttacttgtgt
     1201 ttatacgtca caaggaaaag aaagatacgt ggtacaggga gagctacaat aacaaggttt
     1261 taatcaacgc gagtcgttga aatatgaaat gaggagagta aaaaatggca ataaatcggt
     1321 atgtaaaaaa agctggaatg cttgttggaa tgttaggaat cattgcaggc tgctttcagg
     1381 ggtatcaagt acatgcagaa gatgtcacgc aaaaaacacc accagagaag gtcaatatca
     1441 cggttcataa attaatgtac gatcaaggaa cgcaattgaa tgttgatatt gacgggatca
     1501 aaaacgatgg ttatacgcac gatcaatatc ctacaggtgt aacgaaatac aacaaggcag
     1561 attatggtga tgttgagttt acgttaggaa atattacgga tcaagtatta ccgagagaag
     1621 attctgatct aaccaacgcg aaagtggacg agattgtaca agatgtagaa gacaagggat
     1681 ctaacagtga gtacgtcaag aatgcaacca acaaaattac ttcagctgta gatgaaaatg
     1741 gtgagatcac ttttactgat caaccagcct atgtcaattc caaagggaat gtatatgtgg
     1801 tttatgaaag taaatccgct gctggattag tcacacaaaa agcaaaacca atggttgtga
     1861 ttgcgccaat gacagataaa acaggatcag gatttttaaa aaatattcat ttgtatccta
     1921 agaacattgt ttctaagttg agttttgaat taacgaaatt tggtgacgat ggcgcagacc
     1981 aaagcaaaca aaccctttta aaaggggcaa aatttgagtt atacaagggc gaacctggaa
     2041 aaggaacaaa attgggtgat cttgtatcgg atgatcaagg aaagctgact gcgactgatt
     2101 tgacgttagg aaaatactat tttgtggaag ttccttcgga agttgtagtg ggatcagatc
     2161 aagaaccaac agcagatcaa tatttattag gcgcagacgc tcgcaacgat gcgcacaata
     2221 aattaacctt tgaaattacg aatgatggcg tgacaagtga cttaaaagct tcatacgtaa
     2281 actataaagc accagtgatc gataaaacag taacaaatgg aacaggacaa gaacattctt
     2341 tccaaattgg tgacgcagta aattatcaag gaacgattca tattccaaca gacattgcag
     2401 gtggcgcaga cggtatcaca gtaaacggtg tgaaaagcga aacttctcct tacagtgtat
     2461 ttaaatgggg agatacagca ggtaaaggac tttcttatgt tgctgaaaaa gcagatatta
     2521 aagtaacaaa taatgatggt tcagtcgtat taaaagaaaa tactgactat aaaatcgaaa
     2581 attctgatag tggttttgtg atcgatttta tcgtaaataa tggacaagta agtgatacag
     2641 ttgcgaattt acatggtcaa gatctaaaaa tgacttataa catgtttgtg aatgatagtg
     2701 ctgaaatcgc aaatccatta acaaatagcg tggatttcgt ttataacaat aatccattta
     2761 atcaggaaga acaccacgaa aaaacgaaag ctgacgttgt gacttatggc gcaaaattct
     2821 taaaagtcga tagcggccta tttggaacag gtatcaaagc aacaccgctt gaaggcgcag
     2881 aatttgcagc taagaatgca gaaggtaagt actacggtgg acttactgat acagataaag
     2941 atggtgtcaa agaagcagtt tgggtagacg atgtagcaaa tgcagcaatc ctaaaatcag
     3001 ataaagaggg tcattttgaa atcactggtt taactgaagg agattactca ctggaagaaa
     3061 caaaagctcc tgaaaactat caaaaattaa caaaagagat ctctttcaaa gtagacaaag
     3121 attcatacaa agaagaaaac cgaatcacga tcaaaaataa tcaaaaagct tccgtaccaa
     3181 tgactggtag caacggtttc caatcttacg tattgatctc ttgcttgttg ctaggagcag
     3241 ggggactttc tgcagttgtt tactttaaga agaaagccta gaaggatcag ctaatgatta
     3301 ttcgtaagcc cccaaaaaga agagtcatgc acaaactgat gatatttttt tggttgctgt
     3361 cggcagtagc agtctattta attccagttg tggaaacaaa tgtagcgaaa tttcaacaac
     3421 agcaaagaat tttagccatt gaaaatcttg aaaaaacaga ttcttctttg gatgttgcga
     3481 aaatagagtt aggcgatcct gttggtgttc ttacaattcc aagtatttct ttaaaactac
     3541 caatttatga cggaacttcc gacaaaattt tagagaatgg tgtagggatt acggaaggaa
     3601 ctggcgatat tacaggagga aacggtaaaa atcctttaat cgcggggcac agtggacttt
     3661 ataaagataa tcttttcgat gatttgcctt cagtaaaaaa agggcaaaaa ttctttatta
     3721 aggtaaatga agaacaacat gcctatcaaa tttatcgaat tgaagaagtt aaagcagaag
     3781 aattacaaaa aaattattta gattatctta tgcctgaaaa agaccaagat aggatcacgt
     3841 taatgacatg cacaccaaaa ggtgtcaact cacaccgctt tttagtgtat ggtgaacgag
     3901 tttccttcag taaagaagat ttaaaactac agaaaaaagc aaaaaatagg aaatcaattt
     3961 cattggattg gcgattagca ctgctattcg cttttttttg cgttcctatt atttttattt
     4021 ataaaaaaat aaggaggaga tcggaatgaa taagagaaaa tggctgtttg tttgtgtagc
     4081 agctctcttt tctactgtag cttattcgca actgtctttc gctgacagca gtgatagtag
     4141 taccactcaa acaatagata attcaattac tgcaccaagc gaaacggaca gttcggagaa
     4201 tactacagaa gaaacggcag attcaaatat aagctttact gaatcaacga atccaacgtc
     4261 agaaagttct tcagagactt cagaaaagcc gcaagacgta gaagatacgc aagtgatcaa
     4321 gcaagccatt catattcaaa aaattatttc ttcgaatcaa atcaacaatc aaggcagtca
     4381 agtagcccag caagaaggta tcaatggcgc gaagtttatt gtctacgata tcacgaatat
     4441 cttagaagaa atgtcacaag atgataatga gaaaaatcaa tctgtagaaa taattgagtc
     4501 aaaattgaaa gatcgagcaa aaaaactttc atacgaccag ttgaaaaaag tggctgaagg
     4561 agaaaccaaa acgattgatg atcaggaagg cgtgattgaa ttttccattg aagtacctgc
     4621 aaacaaaaaa caagcatact atatcgttaa tgaatcaagc cctgaaaaca tttcaaacag
     4681 tgaagatatt gttttattga cacctgtttc aaatgaaaaa ggtgaatttt tgacggatgt
     4741 ttggttatac cctaaatcag agaaatcaga gccgaaagaa gaaattaaaa aagttgtgtc
     4801 aactggtgta aagaagaact tttttgaaaa ttgttgggaa ttttttgctc aattattttt
     4861 tgataattag gatgaaatga tgaaaataat aagaatatta gtttttaatc tatccttttt
     4921 agccgcaaca atattgtgtt gcggtttaat tttttctcaa ttatcaattt atccaagata
     4981 tcaacgattg ttcattcatt ttctaaacat tcaatgtgct tccatgccat tagaggtgat
     5041 taaaaagctc ttgatcaatc caatttttat ggttagtttg gttcttctta caggattggt
     5101 aacgtgggta ttaggaaaaa aagaatgaaa agcttacgat cgtttaaaaa aatattagat
     5161 ggagggcatt atggaaaata aaattcattg cactacggga agagtaatta ctttagtgtt
     5221 gtttttgttc cttcttcttt ttgcgagggg aactgcagtc caggccttag aaaaagttga
     5281 tccgagtcaa atcaaagaaa ataataatct ggtgctttat catgatagtg attcattgag
     5341 aaatatcgaa gtaactggga tcaatggatt taagatacta gatgagtatt caactaaaaa
     5401 acaaaaagta cttgcttggc caaaagtcaa agatttgaaa agtacgtcaa aaatcgttgc
     5461 ccattatcaa aaagtaggta cttatctagg aaaagaaatt gattgtgacg tgactttttc
     5521 taattttgta gcgcacgctg gggttaacca agacggatta gctaaaacaa ttaatggcaa
     5581 aaagtattat aattatttgc tctttgataa aaatatttat cgaggatttt tcatgcagtc
     5641 aatcaatgtc atgaatgcaa aactagaatt taagtatgca ggaacaaaaa caaaagtagc
     5701 tttgagtgaa ccacagccag aaattcaatc aaaaatgaga tcgatgagag cagctggtat
     5761 tgatttaaac gatacatttc tatccattta cagtttaaat ggacagtatc agcaacgtgt
     5821 gtcttggtat aatgacaata atgaatctgt cggtgaacgt aatattgata tgagagaatt
     5881 tgttgggtat gaaaaaatgg gtagcttacc ttattttgtc acaacagata cagcatgtgc
     5941 agaatacaaa gctcctgcgt tatcaacaaa caatttaact tcaaaagtag tgggaggacg
     6001 tgcagaaaag gcttatagct cgaatgatca tttcaccgat gttgtaggag ctgatactta
     6061 tcacagaagt ggtgtaacgt atacgcttca aggcgcttcc ccaacattca tgattggcgc
     6121 aaatacgaat agtatgatgt ttagctttga tactgcattg ctatggacac cacaaccatc
     6181 gaagcctaca aaagaagtgt ttaacaaagc taatactgaa gaggcagcac acaatattga
     6241 caaaaaagtg attccacaag gatcagatgt ttactatcat attcaccaaa agtttgatgc
     6301 attaacagtc aacacaatga acaaatacaa atcatttaaa atcactgata cctttgacag
     6361 caaaaatttt gatatggtat cggatgggaa aaactatgat ggcgcattgt ggtatgttga
     6421 agcgaatggt acttggaaaa aagctgatac aggaggaaag ttaactgtat caggtaatac
     6481 agtcacttat actgcttctg acagcttttt agctaacgct gcaaattacg gtagggatta
     6541 tattttgcag ctccatatga gagcaaaaaa tgatgcaaaa ggaattgcac caaatgtggc
     6601 tactgtaacg tttaacaatg atccgaacaa aggaacacaa aaaacaaata cagtagaaaa
     6661 ctatttctct cttaaaccaa ataaaaaagt ggaacaagat gggaaagatg ttgatggtcg
     6721 aaatgatggt aaaaaaggaa cgcctacagc tcctttaaat gcaggatctg aagtacaata
     6781 tctagtcaca caaaaatggc atacaaaagg tgtagacatt gcctcagatc attacaagca
     6841 atttagtatt caagatccag tggaagatcg tttgacgtat aaagaaggtt ctgcgcgagt
     6901 gatagatatg agtacaggaa aagatattac ttcagaaggt acactaacct atgatagcaa
     6961 ttcaagaacg ctgaagtggg aagcttccgc tgatttctta agtaaaaatc ttttagatgg
     7021 acgagaaatt cagctgatat ttacagctaa aactcctttg aatgaagaat tggatatcga
     7081 caaccaagca aaagtttcag tagaaagtat tgagaaagca actaatgtag taacgattgg
     7141 ggtaaaacct aacttacctc agatcattgt gccaaaaact ggatcgaatc atttagtgct
     7201 aactgttatt ggaagtatat tatttcttac tttagcatca ttgggctatg tagttttaaa
     7261 actcaaataa atataaaaac aaggctgaga atcagccttg ttttctttta tttgaagatt
     7321 ggattcgcac taataaatac attatctacg ccaattctac cagcagagtc aactacaaaa
     7381 ttatgaccta atccgagaat actttggtta ttataaagta aatttgcatt atgggcaggg
     7441 ctatttttcc atcctaccat ggcatcgata gcaccatcgt ctgataaaag gtcatcaata
     7501 tacagcccag tttgacttat gttttctccc ttagcaccgt agcagcctaa gctttccaat
     7561 aaatcttgga aagtaccacc accagggcga tagtgactga agctgacttg taattcttta
     7621 gctcgaatat ctgcagcctg ttgaacaata tcattgaatt gcattggggc aacaccattt
     7681 tgttggcgat aatcatttaa ccaagcaagt tctttttgac gcataaagtt acgtaattcg
     7741 tctaatgaaa tctttttata tttgaatgtg attgttttat tttgatcgac gttattaatg
     7801 gatcgttggc cgtttttatc cacggtaacg gtaggatcgt catttctgca caagaaataa
     7861 ttttgatcag tcatattttt ccaaggagta gagaagctat ccccttcgta tactgttttt
     7921 gattcactag agagaacagc gttggtatca gcatttacat gattaacagt cacattaaat
     7981 ttacgagtgt agttgaaaat ataattttga ctttcagtca cattgtttaa ggttactgaa
     8041 tttgttggtt ctacccgatc gtctaaaaca tatccttgaa tagaaggagc agttaaagtg
     8101 acactttcac cgtgagtgac gttgattatc tgttctttca aaatatcaac tcgcccctca
     8161 tttttgtagt tgttattgca caacaaattg atttgatgca cttgattata attgaatgtg
     8221 attgttgtat tacctgtgat tgttttgctt tgagtatcat taccaatcac tttgtagcta
     8281 aaattatctt tagcaccttc gctatattta aatgcttttg ccttagcagt gtaagtagaa
     8341 ccagaagtca cttttgcttg atctgtagca atcacttgac ctgtttgatt gtcaatatgg
     8401 ttaatggtaa ccatatattc ttttggcgtt tcaggtttgt gttgcaaccc tttaaatgca
     8461 gcatcaagac tatttttagc attgtttact tgtgtttgag aaaccttaga atcgtctagt
     8521 acttttttag cattggataa agcagtctga aaattattcc aagattcttc tgtaaaatct
     8581 cctttagcag ttgattttac tcggttgtat aaagcttgta attcagcttt attcacagta
     8641 ggaactggtt ttttctttaa tcctttgtaa gcagaatcta atttattttt ggcactatcc
     8701 acttgctttt gagtcgcttt tgagtcattt aacactttct tagcattgtt taaagcggtt
     8761 tggaatgcgt tccaggtatt gtcggtatag tcaccttttt tcgttccctt aacgctcgta
     8821 taaagagctt ctagagccga tttattcact ttgggtttag aggtgtcagc gccataccaa
     8881 ccgatacctt cgtatttcca gccatagtct ttggttaacc attgtacttc tttccaactt
     8941 gcagtgtagt gatgtgagcc agattttaca ccaggaagga aaaggcggta gatcggttta
     9001 ttattttttt cggcagaacg ccaggcaggc ccttcataag tccagccgta atttttagtc
     9061 aaccatttca cttcatccca actagctgtg taatggtgat cacgaagacc tttattgtac
     9121 aaacgataga caagatggcc accgctagtc ggtgcttccc aggcggggcc ttcataattt
     9181 ccccatttta tctttactag atggtcacgt tcttttttgc tagaggtgta aaaatgttca
     9241 ttattatttg gattgtaaag gcgatacatg acgttagttg aagcagcgtc aacagatcca
     9301 gcaagactaa gtgaggcagc aaatgttcca atagctaaaa cagtttttgg attaaataat
     9361 ttcatttttt caactcctaa atttaattaa aaatgtttac atgattatta tatatcggct
     9421 aaccgatata gtcaataaaa aataagaaaa gccttatata attttggctt ttcttatttt
     9481 atttgttagt tgaagtatta atatcaacga tttcttcagc caatttattt aatttcatag
     9541 catttatgaa tctcatgttg tctatactag agcgtttgtt ggcaaggtca cttactgtag
     9601 ttaagggtac gccagattct atggatatag aacgaactga tttaccacta tcaaacagca
     9661 accattcgat tttcttttta tcagcgtaca tttcaatgct ccttcttgtt ttttctttat
     9721 tatagctctt ttttgtaatg aatcacaata tcaagagaaa aattttctat agaaaggtgg
     9781 atttttatga gtattcgttc tccgtgtatc caaatgaggt ttgttgctac tttcgttatt
     9841 tgatatacta aaaataaaca acgaaagaag gaataaaatg aagaaaaagc aactatggtt
     9901 tgtctctatg tttgcaatat tttttattat tttaagtgga tgttcaagta ataagaaatc
     9961 ggaagctaaa gaagataata gtttattgca accaggaact gtttggaaag aagaagtagg
    10021 tggtcttatt cataatttaa aaattataga tgatactacc tgggaatatt ctgaaagtgt
    10081 atggcatcca gatcctgtac aaataacagt taagaggcaa aaagactatg aaggtttaga
    10141 aagatacaaa atagtagatt ctgcaggcat tagggaattt ataaacaaac ctgattcatt
    10201 atttattgtt gttccctatg agaaggatgg cgtaaaaaaa ataatttttc ttggatctag
    10261 caaggatgag aagcgaacga aagagaaatt aattcatgat ggatcagagt taaataaata
    10321 caaattacaa aagactagtg agtgattgct agtcttttta tttatcctga aggaggaatc
    10381 acagtggcaa aagttaaaac accattcgaa aaagccgcgt ctaaaatctt agaacgccgt
    10441 ggtgtttctg aaatacagtg gaaaaaagaa gttcttgaaa aagcacaatt tgacctatta
    10501 cgaggaaaag ataaggactt agaagaatat atctatcaac gtgaacgtga aaaacttgta
    10561 ttcgatgaaa ttattaaaca ttaaaaggag cgaataatct tatgtttgaa tcaattacaa
    10621 tgaatttttt aactcaaccg aaagtagtag ctgcagttga tatttttaga gcattacaaa
    10681 ctgccgcaac tagtttgaaa aataatctga tgatttttgc tgctgttgcg gcagtggctt
    10741 ccattgctct tggaggtatt ttcttcttct ttgggcgaag aggagctgaa aacgggaaga
    10801 cacacgtagg ttatgtattg ttgggggtag gagttatttt agctgccgct tctattgtag
    10861 tttcaatgtt ctcaatgttt ggacaaaccc cacaaggact ataaaaaaaa caagtaggag
    10921 gagtaaaaag tacgtggcta ctttttactc ctttttttat gaaaggagaa tattcatgtt
    10981 ttttacgatt tcaggaaatg caaaattgga taaagagtta ataagaaaag caacgaagaa
    11041 agtgggaaag cgtacttcct ttttaccgaa aacaattaaa tattcggtca ttgaaacgga
    11101 agaacagcta catgtgttag gacaagtgtt agaacaagag aatcaatctt ctgttaaatt
    11161 gactctcacg ttacatgata aagaaggaaa aatagaaaag gctggcacag aatacgtttt
    11221 tggcccactg gattatcaaa tttcagaagg tttttatcct acattgatag atttaatccg
    11281 ccttgatgtg tttaaagata cggatgaaac tatgagtgtg gaagagtctt atcgaaacga
    11341 aaaactttta caagttttag aagaagagtt agagagcgac caaagaaaag tgaatcaagt
    11401 gagtgaagtc gaagtaaatg aagaagtacc atgggaacga ccaatatttc caaagaagga
    11461 gtcaaaagaa aaagagacag tctatgttga cgaacaagct gatattccga ctcagaaatc
    11521 gatatttgag gaagagaacg atgtgatggg tcaattggag gtagatccct ttgaagaaac
    11581 aatggaggat gaagagacgg acgaagtaga agttgatcca gttgaagcta atgaagagtc
    11641 aacatggact gaaggggatt ttcaggaata tattgaacca cttatcagtg aagaagtaga
    11701 aaaaaacgaa acagagatac aaaaggcaac tgtatttgat tatattttgg atcaatatcc
    11761 gccagagcat gaatggatga aagaaaaaaa caaattattt ttaaatgagc tttattcgaa
    11821 atatcaatta ccccaaacct atcaagcatt tttagagagt aaagaagaat taatctcaca
    11881 gggtaaaggc acacttttag aacagttaga gtctgtccaa tcaacaaatt ggcatgagct
    11941 tggacaagaa gatttattag ccacctttga agaacgtcaa caagaaacag atcaagaaat
    12001 taatgagtat gcgacggaac agtcgtctaa ttgggagaaa gtaaaagagg aactaaataa
    12061 agaagaacag cgagtgattg aagaagaagt acaaaaagtc aaagagcggt acgaaaagaa
    12121 acgcgacaat gcttattcct tgtgtgtaga aaagatcaac aattttacta agacgaatca
    12181 agaagcgtta tcgaaagaaa aagaaaactt gttaaaagaa cgtattaaag aacgaaaaga
    12241 agagttctac cgcggtttac gtacagataa gctaaagatt agcaaagaat taaatgaaca
    12301 actgaataag ctatatcaaa caacgactga agttcttatg gaaaaacacg aacagatcca
    12361 gcaagaatta caagtacaaa ttccaaaatg gaaaaaagag tatgaaattc aacaacaaga
    12421 attgaaagaa caagaagaaa aacaacggca aaaagaaaaa gagcgtgaac aattagaaat
    12481 aagaaaacgg gaacaggatc ttgcggaaca aaaaatggcg atagaaaaac aacggttaga
    12541 gcaagaaaag gaaaaagaag cagctgctcg taaggaacgt caagaacaaa tggatacatt
    12601 gcgtcaagca cagttgaatt atctagcaca acctcaacca gtagctgctg ttcaaacgcc
    12661 agtgaacaac caagcaaatt taactccttc accccagcgt tcctttaacg gatggatggt
    12721 gggatgtatt gcaagtttaa gcttgcttct aggtggagga acagtatttg cttttaatca
    12781 tatcaatcaa tcttcacagg ctgattctgt agccacaacg gcgtcaatca aggaagaagc
    12841 ccaggaacaa gcaagaaaag agtatgaaca acaattagaa gaattattga atcgtaccaa
    12901 tcagtcaaca acagagtcaa gcatggaaaa tagcaaagag agtgattcta gtaaggctaa
    12961 agaaggatca agcgattcaa ctgtacaatc aaacacttcc gaaagttcag aatcaaatga
    13021 ggtgaaataa tgcgtgttag agaaacaacg aagcgaaaag acaaacgatt caatttagaa
    13081 aagaataggt atctttctca tttctatcgc tatactccct caggagaaga gctaccagaa
    13141 aaacatgaca aacgcatttc gaaaaagatt ttttatagtg gacttgtata cctcccttta
    13201 gagtatttag taacaagagt accagaagtt ttcaaccgat tggaaattgt taagggaagc
    13261 ttacaaggaa taaatctgtt tcaattacag gaaatgccac aggtctttca ttttgattca
    13321 gctggttttt ggaatccatt tactcctaca actagtttgg gtacacgttt agcaatttcg
    13381 acagtttgtg tggttccagc tgtacttatg ggattgaaaa tggacatgtg gtttcgtcct
    13441 ctagcagatg gacaaaaagg ggataatcga ttaatgacct taaaagaaat ccaacagatt
    13501 tatccgcaaa tacctgaaac aaaaactact tttccaggtt atggaggtat tccaatcaca
    13561 cattacaaaa aatattggta cattcaaaca gatactgtta atacgtgtat tgttggtacc
    13621 tctcgatcag ggaaaggtca gattgaagtg ttagccacca ttgacaattt atctcgtgca
    13681 gaaaaacaaa gttcaatggt cgttaatgat ccaaaaacag aattatatgt tggtagtaaa
    13741 gatgaactag aagctagagg gtatgatgta tatgccttta atatcaccga tccactgcaa
    13801 tcaatgagtg ataatccatt ggcactcatc gtgaaatact ggaaacgagg agacatagat
    13861 actgcaacac agttaaccaa tactttcaca aatacgattt attatgatgc caacgcttca
    13921 gataacaaat actttaacga aaacgcgcag aaagctgtca atgcgcttat ttttgcgttg
    13981 ttagaacaag ctgatcaaac aggagattac tccaaattga caccgaataa tctcgttgag
    14041 cttctttctg agattggcgg ttttaattat tatccagatc cagacaatga attcaaacag
    14101 ttgaatgcct tagatgaatt tatgaatcag ttgccacctg gaaacattgc aaaaaaacaa
    14161 tatggggcaa cgaaaattgg atctgataaa gcgaagggaa atattttatc aacggcaatc
    14221 acgggattga atccatttac tctaacgaaa attgcaaaga tgacttctca atcaacgatt
    14281 gattataaat cggttgggtt tccaaaatac ttagatttga aattacatga aagtctttta
    14341 aatcaacgag tcgtagtaga attttatcat aagggaaaga aatttcatga agaacgtgtc
    14401 aaagtaggct ataaaggatt tagtgaaatc aattttgatt gtcatttagt tgcaggtgat
    14461 caagtttgtt taaaatacga tcatcaagga aaagaatatg tggcttggta tgaattttct
    14521 caaagagtat tgaaagacga agagggtaat atcgtttatc ggaaaaaacg aggagaaacc
    14581 catttgccag aattagaaaa agaagccgtt ctaacattag atgaggaaaa aagtaactta
    14641 ttagtagagt ctattcggat gcactatacc gataaaccaa tggctatttt tatgcttaca
    14701 cctgattatg atccatctaa ccatatcatt gttagtattt ttcttagtca gttatataaa
    14761 gaattatcta cgcaatgtgt taagactaaa ggagataaat gtcaccgcag gattcatttt
    14821 attttagatg aatttgggaa tatgccacca atggataata tggaaggaat catgacggtc
    14881 acagctggcc gaaacatgtt gtgggatctg tttattcaat cttatcagca actttatgcg
    14941 aagtatggag aacaaaacgc agcgattatc aaagaaaatt gtcagacaca tatttatatt
    15001 atgtcgacag atgataatac catcgaagaa ttttctaaaa aagtggggaa taagacggtt
    15061 gaacaagaga acagtaccat caattcatta ggtatgaata ctcatatcaa tcgcaatgtt
    15121 gattcagatc gtatcttaac tcctgaaaga atttctactt ttttagaagg agaaacgatt
    15181 gtactcgctc ctttaaaacg acaagatttg aaaggtcata aaatacggcc gtatcctatt
    15241 tttaatcatg gggaaacgaa tcttccttat gcttatcaat atattgaaga tttcaatcca
    15301 tcgtgggata tcaatgatat agacatacat tgtacacatg cggacttgga tcttaaggct
    15361 ttacaaattg attatgatta tttcctacca gatgaatatg ctagaaatct ctatcatgga
    15421 ctgcatgatc aattagaaga aaaaaacgaa gaaaatatgt tttataaaat cgttcaatct
    15481 cttgagaatg agacggctaa aaaattgtta attcaatttt acgaagaaca agcagcagat
    15541 cgacttcaag aatatgttga aaagttgatc aaaaataaag aaatttctga aatgattggg
    15601 aaacggttgt tattggaatc tgaaaaggtg gtgaaagaat gatcattgga ggaatttttt
    15661 ctggcatagc cgactggtgg tcaggcttat tcggagaagg ggatgcaaac cccaaaaccg
    15721 taacgaaagt cttacaagat tatgggaatt atctgcagta tcaagatgta tttagttgga
    15781 tttggaacac ttttctgtgg ggattgatta aaggattata taatctcaat actttactag
    15841 aaaaaaccat ttatcaaagc tttgatttaa aagacttatt gaacgccgct ggggtaaatg
    15901 cactatttca aagtttgatt agtaaggtat tggctctttt ttgtgtcgtc acattaattt
    15961 atttaggctt gaaatttttt atgagtaaac acccaccaaa agtcaaaaat atcttggtta
    16021 atttatttat ggccatgatc ttgatttttg gtggtagttc tttaatcgat gaagggttaa
    16081 atatcagtaa aagcttttac ggagacgtca ctgcagcaaa taaagcagat aaaacgggat
    16141 cacctgcttt tcaaattatt caaaataatg tgatcgatgt cagtacgtta ctagcaactg
    16201 acccgaataa agtagaatcc ctaccaacag ataaaagaaa tgctttaaca gcgaaaaatt
    16261 ttaaaacagc gaatatcaat gggattatta ctcctgaaca agctaaaggt atggcaggta
    16321 atgataaaag tgggttatca gatatacaaa aggatcggtt aaaggcatta caataccgat
    16381 tggaactaga tgataatggg aaagaaattc ctgttcaaat tagtgacgaa ggcttagcaa
    16441 aatatgtcta tacttcaggg tatcgtcgat acagtagtaa accaggtatt attttagctg
    16501 gtgaatcttc tttggcagta gcctatttat ttatcctatt tacgatcgtt acttgcatta
    16561 ttgagctggt atttaaaaaa ttctatttag tgattgccgc gtcaacggac tttgagacag
    16621 gtcaacggat gaaaagtgcg atagaagata tttctcaatc cttcttgtta ttggcgttta
    16681 cgattttaga attacgaatc tataccttaa tgctttcagg tatcggtgat ttgcatgcgg
    16741 caggaaagat caatggattc ttgtatgtcg ttgctttgat tgtactaacc attgcgctat
    16801 tcaaaggctc tcaatcagtt acgaaaattt tcggcgttga tacaagcctg aaaaacggca
    16861 gtaattctct tcttagcatg tttgccttag gaaatattgc gaaaaatgtg ggtagcggag
    16921 ctaaaaacct tgcaagtgct ggtaagagtg gtttaagcca tttaaatgat attcgtaaaa
    16981 atggatttgg ccatcgaaaa gatgttaccc ctgataacgg ggaagaacaa tcagacgata
    17041 aagctacaac tgtacctaat agtaagaaaa cagtttccga aatgttcggg aaagcgaaaa
    17101 atggtgtttc taaagccgca gaaggtcttg gttacgtcaa tgaacgtggg ttaaaaggaa
    17161 cagctgaaga tcttacagaa aaagccaaag acaccttaga agataaatta gacggcacga
    17221 gagctaaaga attggctgaa gctgtgaaga atccatccga agcactagaa aaacaaaaag
    17281 aaaaagcttc agaagtcaaa gaaggactaa aagatgcgta tggtgaagga caagtcaatg
    17341 cagcagtgaa agctaataca acaaccgaaa aagaaaatca agaaggtata gtgattcctt
    17401 cagtcaaaga tcaccaagaa gcgttgcact ctaatgaaaa ttcgattgaa gagaaaaatt
    17461 ccatagaaga aagaaattca gaagaaggaa aaaatggaca gttaccaaaa actgatccag
    17521 aacaatatcg ctataaacct aacagtgacc ttgaaaatga gaagcgaatt tcagatttat
    17581 caaaagtaaa tcaagaagac aaagcagaaa atgagggaca gttaccaaaa actgatccag
    17641 aacaatatcg gtatagatct gttaactccg aaaatgaaaa acgcatttca gatttaccaa
    17701 aacgaaatca agaagtacaa acggatgagg attccatcgt tctgccaaat gataatggaa
    17761 tcgaagttgg aactgaaaaa tcaacgaaaa ttggtttacc taaggcacaa aatttaccaa
    17821 tgaaagatag tcgtttgaat caattcaagg ataatgatca aagttttgtt catcaatcac
    17881 ctgcaggtac tgttcaaatg aaaccaagtc gtttgcctga aaatggaaat ctgacaactg
    17941 tttcttacaa tttatctgaa agtggtgaga cagggaatgc gtccgttcaa aatgttgtga
    18001 caagagaagc tgcaccaaaa cctattcaaa acgttcatac aggttacaag ccagcaaatg
    18061 tggcatttac tgcaccaaca aaaataaatg tgacaagaga aacaaaaaac aggaacaatg
    18121 cattgaaaga acaactattg agggatatca aacaggatat caaaccataa aaggcctaca
    18181 agttaggcct tttttctatg tatgaaaagg agaaatcatt tatggataaa tatagtaaca
    18241 tgaaaaaccc tccaagattt agtggagaat ggaagttttt cgcttttttc tttttagatc
    18301 tattaacgct tggtggctgc tttttttggg cgaaaatggt caataatctt gtccacttac
    18361 cactcttttt tgcactcttg aattatggct tatggattgc gatagcgtta gtgattgttt
    18421 ggcgacccta taataaccca ggtcaacgac aaatttttgc gattatcgag tctatttttt
    18481 ttgcggacaa gaaccattat catgcgttag atcctaaccg aaattatcat ggtacgtata
    18541 aggaggatga ataaatgcga gtcaaaggac aaaattcagt tgaaaggaaa tccattttgc
    18601 agtttggaaa accagaagag cgctctatct tggacttatt tccatataaa tcctgtacag
    18661 aagagggagt cttaattact agagaagatc attttcaacg ttattatcgt gtagtttcta
    18721 cagacgtgga aggattaaat gaacaagaga aactagaacg aatgaatcag ttgaccaccg
    18781 tcatgcgtac gtatgtgcca acaattaagt tagttagttt gaccacagag acggatctaa
    18841 cggaacaaat cgtgcaaaag cggcaattat tagataaaaa tcgtatggaa cagtccttag
    18901 gaagaaatct acaacgatta aaaaaatatg aaaagaaatt agtcgaagaa atccaggaat
    18961 tgaaacgagc agaacaagaa cgtcctgact taagcttctt tttcgtcatt gaaggaaaga
    19021 caatcaagga tttacaaacc agaaaccgtc agttaatgcg atctagtggc attttagggt
    19081 taaaaccact ctacaaaaaa gaattgatca agattctgta tcgaatgaac aatatgaatg
    19141 atgagtagga atggagaaga gagaatgaaa tatccgttga tatggttaga aaaagatgtt
    19201 ccgaaggaag agaccactag aaaatttcct cacactttaa gcctgggtaa gttgggaaaa
    19261 gggcaatctt ttaaccaagt aaagaatcaa acaagaagag gtgactgtac atgttagaag
    19321 caattaaaac agctttttca ccacaaaatg atcaaaaagt acttgatcca ttaaagcaac
    19381 taggaaatcg ggaaatagaa attgtaaaat ccaaagggta tgatttagat tttttaaaac
    19441 aagtgatgcc acaaggtggg ttaaattttg atgaagaata tgtgacgtat ggtaatggtt
    19501 attcacgatg tgtgacagtt tatcactaca caaaaaatcc tacgcttttt tggttggcca
    19561 atctcatgaa caatcctcat acagtggcca cactcgatgt tagcacagat gaaaagcaaa
    19621 aagtgattga tcaaatcaat cgtgccttag atgaattaaa tgatcgatca gataatgaac
    19681 gaaaagcgac tgaccgaaat aaaagcgcgg aagaatacct ggaactagcg gaatatgcta
    19741 gaaaattgac ttctaatggg gaaatttcta agcaagtcaa agtgagagta catgtttacg
    19801 gaaacacgat tcaagaagta gatgaacgag cgcaggaact gatcgaaaat ctcaaaggga
    19861 tggattatcg tgcggtgact tatctgcata aaaccaaacg agattggcaa tcgatgttta
    19921 ccatgttcga tgaacaagct gaatggttcg gaaatatcaa agggcaatca ctaccatcgt
    19981 taaacattgg cggaggtttc ccgttccacc accaaacgct aaaagatcca ggagggacac
    20041 aatatgggga aaccatgaca ggcggcccat ttgtctggaa tgcgacgtat aaaaatagtg
    20101 tacggttgtc tactaatacc ttgcttctag gaatgatggg agctggtaaa tcgaccgttc
    20161 ttaaaatgat cgcggaagcc catttagctg caggagactt tatttggggc tttgaaaagg
    20221 gaaaagactt tattcctttc ttaaaggaat ataacggtat tatggtgcgt ttagacggat
    20281 ctgacggaat gatcaatcct ttagagatat ttgccacacg gacgtatgat gaagcatcca
    20341 gtttatatga cgatggatca gtcaaagatt tgaagatcaa tgaagcagcg tcctatcaaa
    20401 cacacttaga caaagtggtc tatcaggtac agttagtgag tcctcagcta aaaggaacga
    20461 tgaaagcaga atttaagacc tacttaaatc gtttttatga agagtatgga acagtaccac
    20521 gcggctttac ttcctcaaat tcgcgttcaa atactgatac gcaagtcaca gggaaagatc
    20581 cagaagctta tccgacgttc aaagaatttt tagacttttt aggacaactg gaattaccag
    20641 gtgcttccca agagaaaaag aatcgaaaag aagaaatgga atccatcgta gaatccttat
    20701 gtgagacgta tggcatgatt tttgatggtc atagtacgat tcgacacctt gaccaacaac
    20761 agttggtctt ttttgacatt gactctatta caggcttaga tcgaggggta caacaatgtc
    20821 aaatgtacat ggctatgacg ttgatttgga atcaggcatt gattcaagga agaagacaac
    20881 gacgtttaat taaagaaaaa caaatggaag aacaagactt caaacgcttc ttggcgatga
    20941 ttgatgagtg ccataatggt gcgacctgtt ctgcagtgaa aagctgtggg ttgcaagtag
    21001 tctaaaaact attgtaataa ctcttataac gagaagtcaa atgagtgggt aacgccacaa
    21061 agggcttcca ctgatactcc gacatgcaac aagtagttta tgcagaccaa cgttgtgtga
    21121 agctcggtga agtcggctga aggataccgt aaggtaaaag aaatagtgaa taccacgaaa
    21181 gttctctaga ggtaaagggt gtatccgata agtcgggtgt ccaaaaaaac ttaatatggt
    21241 gagtatgtac aaacgtactg acgaactagc gaatgtactg tccgaaaggc atagctccta
    21301 gaaatgggag agacagtgag caaaaaatca ctcattgtgc taacttgtgg ttgagtaaaa
    21361 gtcgttgtta tgaaagacca tgctgcgtta caggcgtagg agaagttagg gggcaaaagc
    21421 tagcatctaa gttaaggcat gtggatagat ataagggaac aggggaagcc atcaacatgg
    21481 agagtgcata ctcgatgaag tggtagtaag aaatatgtga atgacaaata acttacttta
    21541 gtgatggtga aagtagagtc acgactgatg aaggttctgt aatggaactg gaggaacagg
    21601 ctcgattcgt ctgtaaaagt catgaaaccc gttcaaccat gggattctcg tatgactaaa
    21661 aaggtcacga gccaattgga gcgtgataaa ccatgacaaa agaaacaagt ccaaaagata
    21721 ggaagttgcg gcatgcagaa tattatggca tgacggaaat cttcgataag atatactcca
    21781 aaagtttgaa gggagataat ttcaagaatc taatgcagat tatcatttct gataacaaca
    21841 ttgtcctagc atatcgtaat atcaagagaa atcacggtag tgtgacaaga ggagaagatg
    21901 gagtaacgat taaagatatt gaaaatctca accgtaatga attcgtagaa attgttagga
    21961 agcgattcgc acattataac cctcggaaag taaggcgtgt agaaattcct aaatataatg
    22021 gtaaattaag accgttgggt attccatcaa tatgggatag aatcgcgcag caatgcatct
    22081 tacaagtatt agaaccaata tgtgaagcaa aattcaataa tcacagctat ggtttccgcc
    22141 caaatagatc agcagaacac gcaatagcag attgtatggc ccgaatgaat caaagtcaca
    22201 tggaatatgt ggtagatgta gatatcaaag gtttcttcga cgaagtcaat catactaaac
    22261 tcatgagaca aatctggtct atgggtatac gagacaaaca gcttctctta atcattagga
    22321 aaatgttaag agcacccgta gtacttccaa atgggaagtt gatataccca acaaaaggaa
    22381 caccacaagg cggaatactt agcccattac ttgcgaacat caatctcaac gaatttgact
    22441 ggtggatagc aaatcagtgg gaggaaaaag atgccaaaga gctaaaacat cattttagga
    22501 aaaatggagc ttgggataaa ggtaatatct atcacaagct cagaaagagt acaactctaa
    22561 aagaaatata cctagtgaga tacgcagatg attttaaaat tttctgtaga aattataatg
    22621 aagcgaaaaa agttttctat gcaactaaaa tgtggttaca ggagcgtcta aagttaccaa
    22681 tatctgaaga aaagtcaaaa atcacaaatc tttcaaaaga agaaagtgaa tttttaggct
    22741 ttacgatgaa attagtgacc aaaggtaaca aaaaggttgt taatacgcat ataggtacaa
    22801 aagcacaaag catgattaaa gtgaaactaa aaaaacaaat caaacgaatt caaaaaagtc
    22861 ctaatagcaa agataccttt atctatattg gagtctataa cagtatggtt attgggattc
    22921 ataattactt caaaattgcg actcattgta gtgtagcaat gcgaaaaatt gcaagagaac
    22981 tcagaccaag ttttgaaata cgactacgag aacaaggaat ctcaaaagaa ggacaatacg
    23041 gaaagtacga aggatacaag ccttacctac ggtcaggaca aatacgatat ataagagggt
    23101 ttccaatatt accagtaggg tatattcaac acagacatcc gatgtgtaaa aaaagaaata
    23161 ttaataaata caccgaagaa ggtagggaag ccattcataa gaagcaacaa accgtagaag
    23221 aatggaagat atcgtggttg tgtagcaatc cagtgcttag taagagagca acgattgaat
    23281 ttgcggacaa tcgaatttca aaattcatcg ctcaaaaggg aagatgtgca gtaacaggtg
    23341 aggagcttat cttatctgaa atgcattgtc atcatattat cccttatcat gaaagcaaaa
    23401 gtgatagcta tgaaaaccta gtgattgtta ctgaggaggt tcatcgggta atacacgcta
    23461 cacaaagtga aacgattgag gaacttctaa aatatctaaa attaaatcca aaacaaaaag
    23521 aaaaactcaa tgagttgaga ttaaaagttg gtaacgaaga aatatcttaa taaatatttg
    23581 taggacggga aaacagaatg tcttttatag acgagggaac gccgtatgcg gtgaaagtcg
    23641 cacgtacggt gtggactggg ggaaaagacg gagataatat caaactctta cctatcagta
    23701 tcgattaatc cagattttat tgaaacggta aattatgtca caaactttca acgagaaatg
    23761 cgtaaagtag aagcaatgac tgttttagct acacaatccc cacaagaaat ggtgccagaa
    23821 aatatgtctt ctgataactt ctcgaaactg aagaaagtct ttgaattttc ttctacgaag
    23881 atctttatgc gtatggatga atctatttta cgtcatatag aaggattagt gggtaaatca
    23941 atgacgaact cagaattaga atccattcct caacaaaatc aaggagacgc tgtgatcaat
    24001 tttggctcaa aagaaaccat tcgtgttcat tttagtccga acaaacgtca attaaaattg
    24061 tttgatggag gaaaataatg gaaaaccaaa atgaaagttt aatcaagcag tatgttaagc
    24121 gtcgtgcaaa gcggcgctta tttttgtggt tgtttggtac ttctgcaggt ttgattacta
    24181 ttttgatcac tgtgttcgtg accctatttc taatacttgc ggcgggctcg attgataatt
    24241 cggattcaga ttcaagcagt ggtggcgaag cttttacagg agaatattcg gaaggcttac
    24301 cgatttataa agaaatcaaa ggaagaggcc ctttttctga tgagattgcg caatatgccg
    24361 taggtgcagc agtgaaatat aaattattac caagtgtgat cctttcgcaa tacggttacg
    24421 aatcagcgtt tggtacatcg gcgtccgcaa gaaatgatct caactatttt gggattactt
    24481 ggtttgatgg ttgtctgttt cctaaaggaa ctgcacgagg aattggtggc attgaaggtg
    24541 gctggtacat gaagtttccc aactcaaaag ctgcattttc ctattacggc tttatggtag
    24601 ccacacaatc caattttaat gcttgtgtag gaaataaaag ccctggcgca agcttattga
    24661 tcttaggacg aggcggttat gccgctgcag gaattacgga agatagtccg tattatacag
    24721 gttgtatgtc aattatcact tctaataaat taaccgaata tgatgaattc gccatcaaac
    24781 attggggtga aggtggcaat aacaacggca cgattactgg tgaatggacg aatccattcc
    24841 ctggcagcag tttagataaa agttccttca gcggtggtca attatttgga acgaatccag
    24901 gcggtgaatt ccgtccgaac ggcttccatg atggattaga ttttggttca gttgatcatc
    24961 caggaagtga gattcatgcg gttcatggcg gaaaagtcgt gtacgttggc aatcctggta
    25021 tttcaggttt gggcgcgtgt gtgatcgtga ttaattacga tggcttgaat atggtgtatc
    25081 aagaatttgc aaatagtaca ggaaattctc gagtgaaagt aggcgatcaa gtgaaagttg
    25141 gtcaagtaat tgggatacga gatacagcac acttgcattt aggatttact cgaatggatt
    25201 ggcgtcaagc acaaggacat gcattcattg atgatggtac atggatcgat ccgttaccat
    25261 ttttgaatag cagtaaaaaa tagatcagga gaaaaaatat ggaaaataaa acgttaaaaa
    25321 tcgtcttaac agctgcaatt agcgttagtt taatcggcaa tattgcgttg ttttggcagc
    25381 tgaaagagga acaagcagca aatacctata aaattgaaga agtggctaaa aacgcgtctg
    25441 agaagctcaa aaaaaattcc tctgaggcac aggcgaagat cgacaagtta aatcaaacca
    25501 tcaatgagaa aaaccaagaa atcgatcagc tgaagcaaca agatggcaca aaagcaaaag
    25561 aggaagtaac tgaagcacaa agaaagacgg cagaagagtt cggtcaattg gccattgata
    25621 aatcattaag cagaaatgaa ttaacggaaa aactaaaaga tgtcgcaacc caagaagtga
    25681 tcgacaaatt aagtcctaca gacgatgatc accaacatga tgattacgga agttactcct
    25741 ttacattagg tgacgtgcaa acttacgctc aaacaagcag tgtaaaagag gaacaaaatt
    25801 ttgtcgtatt tgttgattat acaatcggca atccgcaatt caaagatgtc aaaccacaaa
    25861 aaataaaagg tggattaact gtcactgaaa aacaagtaga tggtcaatgg aaagtcatag
    25921 actttagtta tttcactcgt taggaggaag aaaatgagta agaaaaaagg agaaagacaa
    25981 cgtttcaaaa aacgaaaaag ttatcttaga tggctgggaa tctttatgat tctcggcttt
    26041 ttgattcttg ctagtccgat ttatacgaat cagaaggtag atataccaga tgaggtaagc
    26101 acgaacgcct ttttaaacac gaaagagctg acaatggttt caaaagaaaa aggagcgaac
    26161 cagcaattag tttttgaatt tgcgatcgat gatgaagatc aaagcttgtt aaaagaactg
    26221 gctaatctta attatcgcgt agaagtaaag acagctaaag gggattatca agcaatacga
    26281 gccaaagtga ttaaggtgtc tgatgattat tttgcagtga agctaacgca tgtaccagaa
    26341 gaatatatcg ccatgcgttt aaccattatt cctgaaaaaa ttgatccaaa agtggatatg
    26401 caagaaccac aagatctgat ttattacgtg cacgaagata aggttaaaga tcaagtgaaa
    26461 gatgaagatt atgaacaaca cgcgatcaat tacaaagtga aaggatacaa gaaagaacaa
    26521 aaagcagctc aaaaacaaat ggaatctttc caagcaacca ttgatttgaa tgaagaatta
    26581 gtgaagaagc taaaagatca attgccttat caagtcgcag atgatcagga aaatactgaa
    26641 aacaagatta atagctacca gcaagaaatt gaaacatcga aaacgcaact gaaagatcaa
    26701 gaagagatca ttcaaaaact tcaagaaaaa attgatcgta tgacaaaaga aaagatttag
    26761 aaggacaggt ggagtgaaaa aatgaagcga acaaacttgg ctgacaaaga tactttcgct
    26821 gagcagaaag tattgattaa agcctatctc aaagttgtaa aagctaaaga aatatgtcga
    26881 gaagtgaatt tatctaacac aacaatatct gagttgaaga atggtcgaaa gaatttagat
    26941 actttaaaat tcagatcatt tgaagctcta tatgtagctg ctaaaaaata ttttacagct
    27001 gataacaaat aaaaaacagg agtattttat gtgaaaaaag tatataaaaa atgcttatga
    27061 atcagctata tcaccttaac catttgttaa tgagatggga gaaaacatta accacgattc
    27121 taacgcaaac atcaagcaca taaaacattt aacactcctc ctttctggct gaagatttca
    27181 accaaacagg aagacagcgt atcaattata gcatacactt tttcttaaat tcatttttca
    27241 tatgacaaaa tatttccttc taatcgacaa agttagaagg attttttatt gtctaaatac
    27301 acaaataacc ttaattttta aagagcataa ggaaacgtga tatcactttc tcacgaaagg
    27361 tgttaggtag gctgtgccta ccacgtttct aagaaagata aatgatatca aaattttgga
    27421 tttgtgtgta gatttgtggg taggatgaaa gcgaatgtgg gcagtaaaat aagcgtttgt
    27481 gtgtagcaaa atcaaaa
//
LOCUS       DAHHZJ010000021        26897 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.022, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000021 DAHHZJ010000000
VERSION     DAHHZJ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.022"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(49..393)
                     /locus_tag="NBL02_000735"
     CDS             complement(49..393)
                     /locus_tag="NBL02_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HCD2571442.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(530..1675)
                     /locus_tag="NBL02_000736"
     CDS             complement(530..1675)
                     /locus_tag="NBL02_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCD2571443.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(1855..2829)
                     /locus_tag="NBL02_000737"
     CDS             complement(1855..2829)
                     /locus_tag="NBL02_000737"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCD2571444.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(2909..3784)
                     /locus_tag="NBL02_000738"
     CDS             complement(2909..3784)
                     /locus_tag="NBL02_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HCD2571445.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            3914..5821
                     /locus_tag="NBL02_000739"
     CDS             3914..5821
                     /locus_tag="NBL02_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HCD2571446.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(6362..9640)
                     /locus_tag="NBL02_000740"
     CDS             complement(6362..9640)
                     /locus_tag="NBL02_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HCD2571447.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(9911..10456)
                     /locus_tag="NBL02_000741"
     CDS             complement(9911..10456)
                     /locus_tag="NBL02_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCD2571448.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(10468..11160)
                     /locus_tag="NBL02_000742"
     CDS             complement(10468..11160)
                     /locus_tag="NBL02_000742"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCD2571449.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(11179..11463)
                     /locus_tag="NBL02_000743"
     CDS             complement(11179..11463)
                     /locus_tag="NBL02_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571450.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(11476..12039)
                     /locus_tag="NBL02_000744"
     CDS             complement(11476..12039)
                     /locus_tag="NBL02_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCD2571451.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            12267..12929
                     /locus_tag="NBL02_000745"
     CDS             12267..12929
                     /locus_tag="NBL02_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HCD2571452.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            13053..14249
                     /locus_tag="NBL02_000746"
     CDS             13053..14249
                     /locus_tag="NBL02_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HCD2571453.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(14350..15726)
                     /locus_tag="NBL02_000747"
     CDS             complement(14350..15726)
                     /locus_tag="NBL02_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD2571454.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(15763..16629)
                     /locus_tag="NBL02_000748"
     CDS             complement(15763..16629)
                     /locus_tag="NBL02_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HCD2571455.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(16718..17743)
                     /locus_tag="NBL02_000749"
     CDS             complement(16718..17743)
                     /locus_tag="NBL02_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCD2571456.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(17761..18435)
                     /locus_tag="NBL02_000750"
     CDS             complement(17761..18435)
                     /locus_tag="NBL02_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD2571457.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(18619..19512)
                     /locus_tag="NBL02_000751"
     CDS             complement(18619..19512)
                     /locus_tag="NBL02_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCD2571458.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(19586..21283)
                     /locus_tag="NBL02_000752"
     CDS             complement(19586..21283)
                     /locus_tag="NBL02_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCD2571459.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(21276..22589)
                     /locus_tag="NBL02_000753"
     CDS             complement(21276..22589)
                     /locus_tag="NBL02_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HCD2571460.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(22589..26107)
                     /locus_tag="NBL02_000754"
     CDS             complement(22589..26107)
                     /locus_tag="NBL02_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCD2571461.1"
                     /translation="MDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEK
                     KEISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMV
                     NFYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETL
                     YKDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDV
                     LFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGS
                     SNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAI
                     GRNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVS
                     PFGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYK
                     FSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSS
                     SIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFET
                     KMPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEY
                     TSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNI
                     NKLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDG
                     KYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRL
                     YDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTL
                     DGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQP
                     LHLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIID
                     GVLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPND
                     SSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTL
                     KVEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYIL
                     LAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(26400..26858)
                     /locus_tag="NBL02_000755"
     CDS             complement(26400..26858)
                     /locus_tag="NBL02_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCD2571462.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT         7551 a         5898 c         4767 g         8681 t
ORIGIN      
        1 tctaacaata ggccgtttca aaactagttg tctttttata tccacagatc attcgacttt
       61 ttcgaataac ccacggctaa tcatcccatc catcagaaaa taaaatttat cttggctcat
      121 tttctgtttt ggatctttga aaatattgac tgctttcaat ccatttgctg tagtgatcga
      181 cattttcaat gtttgaatat cttttgatac agtgattttt aatttgaatc cttctttgct
      241 ttgcggcgtg gcatctaatg gtcggatcaa ttgataattg cctccgtttg tttctgtaaa
      301 tccattatcg cgcaatgtat atctttttgc atcaggatgc aaacgatagc taattggtgc
      361 gcccagtacc gttgctgttg tttcaaatgc catgattttc acctcgataa aattagtttc
      421 tctttattat aaaagtttat cctgattttt actaggtttt tcagttaagt tccctaaaaa
      481 atgaaggcgt tctctcagac atctgttcat ataagaatga cttcactagt tattttttga
      541 cagcacgaac taattgatca aggaaatact ggatgtcgtc ttcggtattt cccaaaccaa
      601 agctgatacg gatggattct tttagtatcg gtgtatcttc tccatacatt gcttctaata
      661 cgtgtgaagg ctcgatattt cctgctgtac aagctgaacc agtagaaata gcgaccccat
      721 taagatcaag cttcatcagt agcaaatcat ttgcttcgcc aattaagcga agattcaaca
      781 catgagggag tttattcgtt aaatcaccat ttacttgata agggatttcg tttgcttcta
      841 atgtatcaag aatcaattgc tggaatcctt tatatttgtt gttacgtgct tcacgttctt
      901 caggtgacag cagttcaact gcttttgcca taccccagat ccctgctaaa ttttctgtac
      961 ctgcacgccg cttttcttcc tgctccccgc ctaaaagcaa tgcagggata tttactccgt
     1021 ctcgtttata taagaaaccg acgccttttg gcccattgat tttatgagca gagatactta
     1081 ataaatcgat tcctaattct tctggatgga tcgtttggtt tccgtacgct tgtaccgcat
     1141 ctgtatggaa ataagctgga tgatccgcta aaagttcacc gatttctttg attggcatca
     1201 aatttccgat ttcattgttc ccgtacatca cagagaccaa gatcgtatct tctcgtaacg
     1261 cttttttgac ctcttcgatc gacagattcc ctttttcatc tacaggcaga tatgtaacgt
     1321 caaacccctt tgtctctaaa tatttcatgg tattaagaac agcaggatgt tcgatagctg
     1381 tagtgattag atgtctgcct tctttttgac gcgagaacgc tgtttctaag atagctgtat
     1441 tatccccttc tgttccccca ctgttaaaga tgatctcgtg aggttttgct tgcagactgt
     1501 ctgcaatgat ttgtcttgct gtttccaatt tttcatgcgc gtggcgacca aatccatgta
     1561 tgcttgaagg atttccaaac gtgtcttgca tgattgccgt catcgcttca attacttgtg
     1621 gatgcatggg ggtagttgcc gcatgatcca gataaatagt tttcacttca ttcatccctt
     1681 ccaaattatt gctaggcaca ttctagcata aaatagcgaa caaaccaaag aagagctcat
     1741 ttttgtctag aggacgtttc atgggcatag aaaaaaggag ctgtgacaaa agtcttgtcg
     1801 cagctccttg aagctgctca cttctgtcac aacttctttc cctctttttt ttgtttattc
     1861 gaattctttt tcttccagac ggaataacgg actcatcggt gtattttcat ggatgcgttt
     1921 gaccgcttca cccattaatt ctgaacatgt aacgattgtt aagttttctg gatgacgatt
     1981 ttcttctgtg aaaacagaat cagtgataca aatatctttg attggtgcat tgtctaaaat
     2041 acttttcgca tgatcggaca ataatccatg tgacgcacag gcatatactt cttttgcgcc
     2101 gttttctttt aatacatttg ctgctcgtgc aagcgttgct cctgtgttca agatatcatc
     2161 taccatgata catgtctttc cttgaacatc accgatcaca taaccaccct cagttgaagc
     2221 atcatctgca tgatctacga tggctaatgt gctgttcaaa tattctgaaa gactacgtgc
     2281 acgctgaaca ccgctgtttt ttggcgaaac aactacgatg tcatctcccg tcagacctaa
     2341 ttgacggtaa taatgagcaa acaacggcat cgtaaataaa ttatctacag gaatatcaaa
     2401 gaatccttgt acttgaaccg tgtgcagatc taaagtcagt actcgagttg cccctgcttc
     2461 ttcgatcaag ttagcgatca atttcgcagt gatcggttca tgaggtttcg ccgtgcggtc
     2521 ttgcctagca tacccatagt aaggcaatac gacattgatc gttttcgcac ttgctcgctt
     2581 cattgcatcg atcatgatca gtagttccat atagtaatca ttgacaggtg cattcgttga
     2641 ttgaacgata tacacatgat cccctcgaat actttcttcg atattgatgg aaatctcgcc
     2701 atcactaaat tgcttcacga cgcttttacc taattctgtt ccgaacactt tcgcgatttt
     2761 ttcagctaat ggacggttgc cattcaaact aaaaatcttc aacgtgtcat cctgatactt
     2821 attactcatg tagtcctccc caaaacttct ctattcttcg atagtttaac atatttttcc
     2881 aagattttct gttaatttca gcaaattctt aaggaagtga aactgttccg ttactccaac
     2941 tggcaggaat gactttatga taatcgcccc agttgtcgtg tactttgtaa taacgttctc
     3001 ctgtttcagt ttcaaaataa gcgtacgctg tcacccaatg attgccatat gtactgccaa
     3061 gtactttcaa aataccaatc atcacaggtt tgccttcacg tatacgtttt gtagctcgct
     3121 gccatgaacc tagaagagtt gctcgtgcac gctcatgatt gccattcttc tttaaaaaac
     3181 tagaaatccc tgtactaacc tgaaaaggaa tcgtaggaag tcctagtggt tggatctggc
     3241 tacgcaatgc cttcgtcaaa accaacgaat cagccgtatt tttcttgcga atctcatttg
     3301 gaagcatcca ccccttacga tagtcttgat aataggcaag gagaactgct gccgcgtaag
     3361 taccacaaag atagcctgag ggggtagccc atttcttata cctcaaaaaa cgttcttctg
     3421 gcaatcccac ccagcaattt gcgagttcct ttcctgacct tttttcttcg atcaaaacgg
     3481 gaatatcttc ttttctgttg atccatctag ataactcgat tggctcttca ttgatcagct
     3541 tccatttttt acaagaaaga ccctcttgat aagtatattt tgctggagaa tattctaccg
     3601 gatgtcctga ttgatcgata atcacataac tttgatcttc aaaaaccaga gctaaaaaaa
     3661 gcaatttact ttttcttgac tgcagctgct gagtgctggc taatttttta atttttttat
     3721 ctactttgaa atataaaggt ttatccacta cttgattcag ttgggatcgc ctcctttttt
     3781 tcattataag tgaaggattg aaaaaaacaa atcagtttga ttgttcgaat tttcaatttt
     3841 ttatgttcta tttgttaatt ccttcatttt gtaagataat gggcacacga aacaaaaaag
     3901 gagcgatcga tgaatgaaca aagaacgatt gaaacaagat ttctatgaag cagtcaatga
     3961 agaatggcaa aaaacagcaa aaatccctgc agataaacca gctacaggcg gtttccaaga
     4021 tttagttgac gggatcgacc aacttttgat gggagaaaca gacgatctgc ttgcccatcc
     4081 agaaaaagtg tcttcagaaa aaatgaaaca tttccttgca tattatcaac tggcaaacga
     4141 ttatgaccga agaaatgagt tgcatgctca accgcttcaa cctataatcg aaaaagttga
     4201 atcattgcag tctttcgacg aattgaataa gcaatttcca gaatggctgt tggatacatt
     4261 accattgcca tttaatctgg atgtagatgc agatatgaag aatgcgcaaa cgaacgcctt
     4321 ctttgcttat ccgccttctt tattccttcc agataagacg tattatgcat cagaccatcc
     4381 caacggtgca caactaatag gcgttttctt tgatatggct gttcagttat tagagcttgc
     4441 cggcaaagaa aaaagtcagg cagaagcaat cacggaacaa gcagtgcaat ttgataaatt
     4501 gattgctcct catgtaaaaa gtgcagaaga aaacgcagat tacagcaaaa tgtataatcc
     4561 gcaatctttt gatacattta ttaaatacac agataaactg gacttgaaaa aacttgtcac
     4621 gggtttgatt gggacaactc cagataaagt gatcgtaact gatccagtgt acttcgatca
     4681 tttgaatgac atcctgacag aagctcattt tccgttattc aaaagctgga tgatcattaa
     4741 aacagtacgt tctcttagtg gttatctatc agaagaattc cgccaagtca gcggaatcta
     4801 ttctcgcgta ttgtctggaa cagacgaagc aatgcctcag aaaaaagcag cgtattacct
     4861 tgcctctgga caattcgatc aagtagttgg cgattactac ggaaaaaaat attttggcga
     4921 agctgctaaa aaagacgtag aacaaatggt gaaaaaaatg atcgccgttt ataaaaaacg
     4981 tctagaagaa aatgactggc ttggggatgc aacaaaagaa aaagccgtca taaaattaaa
     5041 taacttaggt atccaagtag gctaccccga taagatccca gcattattcg accagttcaa
     5101 aacagtacct gcagatcagg gtggtacttt gctttcaaat actcttgttt tcagcaaact
     5161 cacattaaaa gatcgtttca gcaaatggaa caaaccagtg gatcgttcag aatgggaaat
     5221 gagtgctgac acggtcaatg cttattatca tccatttcgg aatgtgatcg tctttccggc
     5281 agcgatcctt caagcaccat tttacagtct agaacaatca agctctgcta attatggtgg
     5341 aatcggagct gttatcgctc atgagatttc ccatgcattt gataataacg gcgctttatt
     5401 tgacgaatat ggcaacttga acaactggtg gacagaagaa gatcttacac aatttcaaaa
     5461 caaagcacaa gcaatgatcg aagaattcga tggtctgcct tttgctgacg gaaaagtaaa
     5521 cggaaaatta accgtttcgg aaaatattgc tgatgcaggc ggtctaagct gtgcattgga
     5581 agcagcaaaa acggaatcag atctttctat cgaggaattc tttgtcaatt gggcaacgat
     5641 ttggcgtaca aaagcaaaaa aagaatacca acaactttta ctgcaaatcg atgtccatgc
     5701 ccctgctaaa ctccgagcaa acgtgcaacc aaaaaacctt gacgaattct atgaaacatt
     5761 tggtatcacg gaacaagacg ctatgtatct agcgccagaa aaaagagtgc gaatctggta
     5821 agaggttgtg ccagaagtgg tcagcttcaa gaaataagaa ggaatttggg aaaatcgctt
     5881 ctcaaatttt tgtgacattt cggcttattt ttcgaagaag ttacttctgg aataccatgt
     5941 attagaggtt gtgaaaaagc tgcttagttt caagtagtaa gaaaaatttt gtgaaaatgg
     6001 ctttccacat ttttgcataa atttgactta ctaccgagaa actggctttt gaacaccgtt
     6061 tatcgagagg ttgtgaaatc agcgtcctga cctgagtatt agagaaaaaa acggaaaaat
     6121 agttcctcat attttgccgt ttgttgagct aataccgagg gtcagctgat tgaacaccgt
     6181 ttagtggaga gattgtgaaa aagctgtcct gcatcaagta ataagaacag ccaatcaaaa
     6241 gcagcaagga gaacctcatc tataaaaaga tagaaggaag tgagacatct attggtctca
     6301 tttccttcta tttttacttt cctcagtcaa ttgttttaca tcataaaagg agaaattcca
     6361 cttatttttt tcgctgtttc atgacataaa caactacacc gattaacaaa attcctccag
     6421 ctaatgctag caacgggtta ttaaattctc ccgtttttgg gagtccttga gtgccatttt
     6481 tttcactttc ttttgctgtc ctatccggat gagaaggttt tccatttggt tttccatttg
     6541 gtgattcatt tcctggattt tctctttctg gtttgtcatt tcccgggggc acatcggcag
     6601 tttctcgcac agtgatcgct ttttgctggg taacagattc tgtatgagca gaccattttt
     6661 ccccatcaaa tatttccgaa cgataagtga cttctagcgt atattctcct tttgaaggtg
     6721 ctgaaaaaat agcggtatga ttagaaaatt gagcacttga ctgtgtttca acgatttgga
     6781 tcttttctgg gaaataacgc atatcttcaa agacaggcgt tccttgattt ggtgtgaaag
     6841 ttgacacaaa aactgtaaaa ggcttcagtt caatgatatt tccttctgca ttctccgcca
     6901 caccatttgc ttcttgtata ataatcggat tattttgttc atttggtttt gtctgatttg
     6961 gttcttgtac gattgcatac tctggatatt ccgaagacgg tgaggtttct ccgtcaaaaa
     7021 tagcacctac agataatgga agaccatcca ctctttcctt ttctgtttga cgattccccg
     7081 gtttaatctg catcagtcca tgaacatgga tacttccatt ttcatagcgc gtaacaggct
     7141 gctgaccttc tttcgaacat gtatttagag actcgatcca atcttttgat atggtatgtc
     7201 caagagtatt ttctgattgt ttctctcgtt cattccaata gtagttcaac gagtggtaga
     7261 tttgatcttc gttttgattc aatgggcgga tctgctctct catctcaaga ttctctgttc
     7321 ggaaagataa gataccagaa gcatggtagt ctgtgtttcc tgctgaagag gtataaagag
     7381 ccacattata tgaaccgtta ttaaaacttg tactgctatt gacgataata tctgttccgc
     7441 tattggaatc gattcctttc gcaagatttt cgaaagacaa actattgatc aattcatgag
     7501 ggcccgttaa agaagaaccg cccattttga atccattacc gttaccttct ccctcaattc
     7561 ctggtaccca gccgtttcga taagcaactg agtttttgat gattactttt ccgatcgaac
     7621 ctgtttcatt tttagcaaaa aggtcccaac cgtcatctgc attatgatac gcgatacaac
     7681 cgtcaaatac atttccttct cctattgtca gtttggccgc aaatccatcg gcatcttcaa
     7741 atcctggatc ggcattttca aaagatgtac agttcttgac taagttatgc gcgggccatg
     7801 cttcgaaagg atcatttgag ctaccgctga tttggattcc tgtatttcca ttccgataag
     7861 catgaatctc ttcgatcaaa ttatgatttc cagaaatctg taatcctttt tgcatagctg
     7921 cactatttgt aatgtcaaac cctcgtaaac cccagtagtg actgaacagt gtcatcccat
     7981 ttccagttcc tttaaaatca agtacaggac gcttgttttc tggatttttc tctgcaatca
     8041 actgaatcgg gttttctctt gtcccgttaa cgccttttgg aatcgtcaat cctctttcga
     8101 aggaataagt tccaggcgcc atgacgattg tctgcccagc atatgcgtat ctcaatgctt
     8161 gtgtcagaga taatggattt tgtctgcttc cgtttcctgc atctgaccct tctggtgtta
     8221 cataaactgt cgaatattta ggtttacggt agtccactgt atgaagaatc tcttttgttt
     8281 cataactagt catctctacg tattcttctg gcggatagtt tttatctggc gtaaaactga
     8341 aagtaaattc gttcgttcct tccatcagtt ttgtctgata atcaaattcc cgattcgcag
     8401 tgatagccaa gttttttgcg ttcttaagca cctctcctgt tgctttattt ttaattgtca
     8461 ataatccgtc agcatttgct ctaaaggaca tagtatgatc ttggtttcct gaatgattac
     8521 tagaggtcac agtgtaaatc ggatcaatca agcgagtagg tctttcttgc ccaggaggat
     8581 ctgtcgcagg atctgagtaa gcaacttcga ttccttctac cttgatcgtc atattccttg
     8641 ctgcagcaaa tcctgcataa ataaccgatt catctgaagc gtataatttt tcccagtcat
     8701 acatgatgtc agaagccatt tcttctcctg tttcaggatc gtaataatat gcctctaaac
     8761 cagtattcgt ttttttcatc tctacgtcca gtttagtaat cgagttcgtg gccggaaaca
     8821 gattcccatg accgtttccg aatatattat aagaacctgt ttctttttcc aagaaccctg
     8881 ctgaagtctc taaaggtgtt gtttccactg agacactccc aggagctggc gcagcttgcg
     8941 gatcttcaga tgatattcct gtgattgccc gtgtccccaa tccaagtcgc atcgtatatt
     9001 tgctgccgct cgtgttgacc acttcttgtc tatccgagtc ccagacatat tcgatccgac
     9061 tgcctagtat ggcaaatgaa tttgaataaa atgcctgccc aaacaaatga ttttttggta
     9121 ctgcgtctct taccattaaa gcaaatcctt cttgggcatt cgtataggtc caagattcta
     9181 tttcgatcgt cgcccgcatc ctaaagttga tgtctttggg gattgcttga taataatagg
     9241 tcattccatc tgatccgctt gactggaatt ttccgccgtt attcgtggaa gagagccgca
     9301 cgccgtcttc taatggttca atggcatacc tactagcact tgtgctttct ccgaaaacaa
     9361 cccctttcca ctcttcattt gcttcttcag attgcttttt tgtcttttca ttttctgatt
     9421 ctttcatcat aggatcattt ccttcttccg tatccatatc ttcactagtt cttggtattt
     9481 cctccattgt tgacatccct tcaatgttca cgtgtgcatt ttcttcgttc acagactgtt
     9541 gttcgttagc agcaacttca acgacagaaa ttaaaaccgg ggtactgagc ataaggagat
     9601 tcatcattag cgcactgtat tttatacgtt tttttaacaa gtgtattcct cccgtaacaa
     9661 attagaaagc gtttacttaa agtattacct aatttatttt tactgtcaat aaaaaacaaa
     9721 attataataa gtgtatttta taaaaagaaa aaatataact taagttgttg ataataaaga
     9781 gatttattaa cgtaaaaaga ataagttttt ttaaaataac gtataaaaaa gagcataaaa
     9841 caaagtcgtt tgttttatgc tcttctttta ttattttgtg ctttttattt tatcaaatca
     9901 ctttgttttt ttataaaatt tctgcgccta gtgtggactc aaaatgtctc aatgcccatt
     9961 catgtccaac gggatcgaaa cttgccacgg catctttgaa aatatggagt ttgtatccta
    10021 gattatacgc atcgactgct gtatgcaaca cacagatatc tgtacatacg ccggtcaaat
    10081 aaatatctgt gatctggcgt tctcgcaaac ggatgtctag atctgttcca ctaaaagcgg
    10141 aataatgccg tttgtctatc caataaacat tttcctcttg ctcatagtct tggtataaac
    10201 tttttaaaga gccgtacaat tcccgtccac ccgttcctat cacattatgc ggaggaaata
    10261 agcggttctc cggatgaaag gaatcttttg gatcatgcgc atcaatggca aaaacgacaa
    10321 aatccttttg atcaataaac ttctttgtat gagcagtcag ctttttttca attacttgtc
    10381 ctgctgtccc agtagtcagc tttccatcct ctgcaacaaa atcatatgtg tagtcaatcg
    10441 agatcaatgc gttcatctta gtcctcctta tacgagatgt ttgacaaatt cgatcaccat
    10501 ttctgctgat cgtttgcctg cgtcttcgat aaattcgtca aagctttgtg tcgccgagtg
    10561 atctgctgtg tcgctcattg cacgtacgat caaaaatggg atattgaact gacgagctgt
    10621 ttgtccaaca gcggctcctt ccatttcgca agctaatgct tcagggaaat tcgttaagat
    10681 ttctttgatt ttatctggcg agtcgacaaa tgtatcacct gtaacgatca atccttcttt
    10741 tgcgttcaaa ttcgttgctt ttgctgcttt aaccatttca ctgcgcaaat atgtgcttgc
    10801 ttcataatat agaggcattc ctggtaattg tcctgggttg tagccaaatc ctgttgcatc
    10861 tgcatcaaaa taagctactt tgtctgatat aacgatgtca cctacttgta agccttcacc
    10921 gattccgcca gctgaacctg tattgatcac catgttcacg ccgtattgtt ggatcaacag
    10981 acttgtggta atagatgcta agacttttcc tataccggaa cgtacgacga tcacttcgtg
    11041 gttacctaat gaacctgaaa taaataaagc gcctgcgcgt tcccatgata atggttcgct
    11101 taggttctct ctcaaaattt ttacttcttc ttccattgcg ccgataatac cgattttcat
    11161 ctttttaatt ccttctttct atataaatgc gattgctaaa aaaaccacac ataacattag
    11221 gataacaata ataagagatt tcatcaaaaa agagttccat ttggtcgtct tttcccgttc
    11281 gctgatcctt gttttcgtga ttggtttatt ctttttgctt tctttacgat aaaagctggc
    11341 aatctttttt tcttcttgct gataagctgt ttctgctttt cgctgttttt ttaatgactc
    11401 gcttgcttgt gcttgttgtc gcttgcgaag ttcgcttcgc gtaataagcg gtcctttggc
    11461 cataagccga tccctctact ttcctttggt caatagttcc cagtattgaa tcgcataaat
    11521 cgtctttgca tcacaaatca actgatcttt catcgcttgt tgcgcctctt caagagttag
    11581 atgatacaac tccagtactt cgtcttcatc ttgtgctaaa ggattctcga ccttctctac
    11641 cccttgtgca tgataaatat gaaggacttc atttgcaaaa cctggtgaaa gatacatcga
    11701 agtaagatgg ctcagcgact tcgcacgata gccagtttct tcttctaatt ctctagctgc
    11761 tgtcatttcg ggattttgtc cttctcctgg atcgattttt ccagctggaa tctccaaaat
    11821 cactttttct aaaggttttc gaaattgttt gacaagaagc agacgatctt gttcgtcaaa
    11881 agcaatgatc ccgacaccgc caggatgaaa taccagttct ctcttcgaag ttcctccatc
    11941 tggcaaacga acttcgtcta cagcaacatc aataattttt ccgctataga tttctttgcg
    12001 actgattgtt ttttcttcaa attgctcatt gttcagcata tcttctcctg ctttctttgt
    12061 aatcataact gctttttttt catttttcaa tttttctcaa aaactcctta ttagtttacc
    12121 atagataaac cttagatttt ctctaaatat agaaaatcct tctttagtcc gatactcttt
    12181 atagtagtcc gtatgctaaa ataagtatgt aatcgaataa cgaaaagaat tcaatactca
    12241 acgataaatg acatggaggg tttattatga taaaacgaaa ttggccgtgg cttctagcat
    12301 tggtcttgct atttctcttt gtccgaaaca atagtttttc ttttgttcta ggacttttat
    12361 tgataggtgt agttttagtt ttgtggggac tctttggttc gcgcaggaaa aagggaaaac
    12421 aagaagaaat tcctgcactg actgacgaac ttgaaactca ctatgaaaaa agcggtatga
    12481 cagcgaatga aatttcattt tttcgacaaa caatgaatca aacaaaaaat gagatcaatc
    12541 aattgcaaat gaatatgcag cagacagcca aacttaaagc aatcgatctc cgccatgatc
    12601 cagtcaaagc agcgaaagct ttattcaaag aattggtgaa agaaccaaac cgactgcatg
    12661 aagcaagtca gtttctttat acgcatctgc cgaatctagt tgatttgaca aataagtata
    12721 atgagatcaa tgaccatgag atcaaaaaca aacagaccta tgaaaaacta gaagaaagcg
    12781 cgcagatcat cgaccagctg tcagctttaa tcgcacaaga ttatcaaaaa ttcgttgctg
    12841 aggatctaga tgatatcgac gtagagatat ctgttgcgaa acagagctta aagcgtgata
    12901 ataaatatga accagaacaa aaggattaac tcttttgttc ataaaagaag actcggaaca
    12961 ttcagatgat gtctggctgt ttcgagtact ttttctaaag tagagacatt catgtaagaa
    13021 tcgagaacag attcaacaag gaggaatttt ttatggatca taaaccgact gaaacaaaag
    13081 atgttacacc agttaacgat acacttgaag atttattgaa caacccgttt tcaactccaa
    13141 cagatacgct gaccacttca caacaagcgg agattgatca gctccaagag caacagacag
    13201 ctgctcgttt aattgacaaa ctaccggcgg aacgccaaga acaagctaaa cagctagcag
    13261 caaaaatcga tgctaatgat gcacagtctg tcatcagcta cggatcggct gcccaagcaa
    13321 aattaagtga attctcacaa tcgatgttga atcatgtgca agcacaagat attggtcctg
    13381 taggagattc cttaactgaa ctgatgtatc gtctccaaga agccaatcct gacgaactgc
    13441 gtgccggaga agggaacatc ttccagcgtg tcttcgggaa ggtcaaacaa tcgatctatg
    13501 aagtgacagc aaaatatcaa aagattggtg cacaaattga caagatttca gtgaaattag
    13561 ataaagaaaa agacggtctg ctaaaagata atctgatgtt agaacagctt taccagaaaa
    13621 acaaagacta ctttgatgca ctgaatattt atatcgctgc tggagaattg aaaatggaag
    13681 aacttcaaac aacgatcatt ccagaagcga tgaaaagagc agaagaaact ggcgatcaga
    13741 tggatgttca gattgccaat gattacacgc aattcctaga tcgtctggat aaacgtacgc
    13801 atgatcttcg cttagctaga cagatcacga tccagcaagc accacaaatc cgtttgatcc
    13861 aaaacacaaa tcaggccttg gctgaaaaga tccaagcatc tatcgctaca gccattccgc
    13921 tgtggaaaaa ccaagtcgtg atcgcattga ccttgcttcg tcaaaaagat gcagtaactg
    13981 ctcaacgcca agtctctgaa acaacaaacg acttattgaa gaaaaattca gaaatgctga
    14041 aaatctcagc catcgaaaca gcgaaagaaa atgaacgtgg aatcgtggat atcgagaccc
    14101 ttcaaaccac tcaaaatgat ttgatcgaaa caatccaaga gacattgcgt atccaaaaag
    14161 aaggaaaaga aaaacgtcgt catgcagaag tagagcttgg acacatggaa gaagatctga
    14221 aacaaaaact gttggattta acccaataga tcacctttcg cttgttacta aaacaaacgc
    14281 ccaacaacag tttattacaa ctgttgatgg gcgtttttct tatctttctt ctaacttctc
    14341 cattggagtc tatttctcat tcatcttgct gtgtccatag ccatagccaa aataaatgat
    14401 cagtccaata accattacga taccgaacac gatccatgtc acagtttgca gacgaagcat
    14461 caatacaata caagtcaaga tggagacgat tggcaatagc ggaacgaatg gcactttgaa
    14521 ttctccagct tttggtttgc caaacatttt tctcaacttc agtaagccta gactaacaag
    14581 catcaaatag gcgagcgcca tgatattcgt tagttccgct aatacattca atgggaaaaa
    14641 tccggcaaag aatacagctg ccacaccagc ggcataagtc gcttttttcg gtgtacgatg
    14701 cttttgatcg atttctgtca taaacttagg aagtaagcca tccttgctga cagcaaacag
    14761 taaacgagca agtgaataca tcatcgagat cgttaccgtc agtagcgtta aaattgcccc
    14821 tacagcgatc accgcgccca ctgttccatg tcctacatac ctcattgcaa aagccactgg
    14881 gtcattgact cctagttcag tatacggaac gattcctgtc aagatcagcg tcacgatgat
    14941 gtaaaggaca gtagcaatca gtatcgaacc aatgattcct cttggcacat ctttttgcgg
    15001 gtttttgact tcctcggctg ccattgcaac ggcatcaaat cccaagaaag caaaaaacac
    15061 aagagctgca ccatctagca caccggaaaa accaaaaggc atgaatggct gccagttatc
    15121 aggtttaacg aaaaatattc ctacaatgat aaacaacgcg atgataccaa atttgacata
    15181 taccatggca ttattcaaac gtaatgcttt cttggcttca agcgacacga tgtaagtgac
    15241 gaatatcact acgagcaccg caatcaagtc aatgtatgta ccattttccg gattgtaccc
    15301 gccagttaat gcttgtggaa gttcggcccc taaacttttc aaaaaacctt gcatatagcc
    15361 agaccatccg gaagcaacag aggaaaccgc tagtaaaaat tccccgatca acaaccaccc
    15421 tgtcagccag gcagcaaatt caccaaatac aacatacaga taggcatatg gcccaccaat
    15481 cactggcact cgcgaagcaa attctgcgaa acttagtcca gacaaaatga caacgattgc
    15541 agctaagaca aatgaaagtg acagtgctgg accagcattt tgattggccg caacccctgt
    15601 caccacaaat attcccgtac cgatgatcgc accaattcca agcatgatca aatcagacgt
    15661 ttttagttct tttttcatct cactcgtatg attgagattg atctctttct tacgaaacaa
    15721 atccattgaa gttcctcccc attttaggtt ggaaaacagc gattaaactg ttttcatata
    15781 ccctaaaagg ataccaccta aaatgactaa aaggcaacca acaataacat aaatgagctc
    15841 ttttttcgtt tttctttcgc caaggatgaa aatcccacct aaagtagaga taatgatccc
    15901 catttgtgaa agtgagaagc caacagctaa accaagtgat ttgactgtta gaagcatgca
    15961 gatgtttcct aagccccata ataatccaca gatcatgttt ttccacacaa actgatcgac
    16021 tttaacttta cggaaagcaa agaagcttgc gcctaagatc attccgatac tttgaggtaa
    16081 gataattgac atcgcatcca aattcgccca gttgacgatg atggtatata caccataacc
    16141 aatcgtagag aagatcaatg ctctaaaacc cttaccaaaa tcaagcattt tattatctgt
    16201 atctattttt ccttcgtcat cttgtcgggc agtcaaatac gcaccgctta caagaagagc
    16261 taaagcaata ataccgtata cataatcttt cgtttgtgtc cattcatgga agaaaacggc
    16321 tcccgcaatc gtattcaaaa ttaattgaaa accagtagac aatggtagcc ctacagaaac
    16381 acccatgtac ttcattccat ggaactgtcc attttgtcca acactccaag ctaagccgga
    16441 taaaaatccg atcaagaaaa tccaaggggt gattacagga ctaacaacga aaaagacaac
    16501 taaagaaaac agaaatgcac cgatcgtcat tcctagtgtt tgttgattgg catcgccacc
    16561 aattttacca cttactaaac caatactacc ccatgccaac atagggacta atgctattaa
    16621 aatttccata accaatctcc ttttatttca catgataaga aacttatcag cgaaaacgtt
    16681 aacaacacaa gcactttttt tcgtttctaa cgattttcta acgtttttca ttataaaaat
    16741 ttaagattgg gaaaaataaa gtcattgttg tcccttttcc ttcagttgat tcagccgtca
    16801 aatggatacc taatttttcg gccagatttt ttgctaaata aagtcctaat cctgttgaat
    16861 gtgttttgct aagtcgacca tttctcccag taaatccctt atcaaagatc cgtttcaagt
    16921 cttccgctgg gataccgatc cctgaatctt taactgaaag gctgactcct tgcgcttcct
    16981 tttctatgct cacaatgatg tttccgtgat ccggcgtata tttgatggca ttactaagca
    17041 attggttgaa gataaaagcg atccatttag catcggtcaa aacagtatga tcctcaccaa
    17101 cgatctcgta gcgaatattt ttttggataa aataatttgc ctgactgcgt atcacaggct
    17161 gaatgattcc ttttagtcca tattcttgga tgaggtagtc ccgcgagaaa ctgtcgagtc
    17221 ttgcataata caaaacttgt tctacgtatt catcgatttt tgataattca ttttctagta
    17281 atgtatactt ttgatcgtca atatcgaact cgatcgaacg aactagcaat ttagaagcgg
    17341 aaagtggcac tttgatttca tggacccatg agtcaatata atctttttgg tcttgttgat
    17401 tactgattgc tgattgcatt acttcttggt gttcacggat cagctgattg atatattctt
    17461 ctaccagttt ttcttcttct gtcctcccac cactcaaata attttgcaaa ggggaagttt
    17521 tttgtatgtc ggctaatttt ccccaccatc ttctcttgct caagtaacca cccagcagat
    17581 aaaaaatcag aaagacgccc tcaatcaaag ctaaataagc aattgtgctc aaatcaactt
    17641 tgatgtttgg cgccagccac atcacaaaga tcgtcaatgc aataaaaaac agccatccca
    17701 agatcaatgt ccactgatct ttcaaatatt taaaaaaatt catcttaacc gctcatcctt
    17761 tcagggaacg atatatccct gtcctacttt tgtttcgata tatccttgga tacctgcctg
    17821 ttcgattttt cggcgtaacc gattgatgtt gactgtcaat gtattgtcat ctacaaatcg
    17881 ttcgtcatcc cacagtgctc gcagcaattt ttcccgactt aagatctttc cattgtgctt
    17941 catcaaaata aataatagac gatattcatt tttgctcaaa tcaatgatct ctccgttgat
    18001 ttccatactt ccgttgtcaa cattcaacgt aatgccattg tggctcatca tttcactaga
    18061 taattctgtg tagttgtaag aacggcgaag taatgcattg atttttgcaa tcagtacatc
    18121 gatttggaaa tgttttgtca caaaatcatc tgcccccatg ttcatagcca tgatcatatc
    18181 catattcgta ttcctgctgg agataaaaat gatcggtact ttcgatactt cacgtatttt
    18241 ttgacaccaa taatagccat ccaaaacggg aagattaata tctagtaata caagctgtgg
    18301 ttcttctctt tcaaagtctg agaacacttg attgaaatcg gtcgttccaa aggtttcaaa
    18361 ttgccatttc tcaagttcct ccatgatcaa ttgtcgaatg atctcttcgt cttcgactac
    18421 catgattttt gccataaaaa atccctccat ctttcttctt gctactacaa tatcaaagaa
    18481 gcccttgtga aacaagttgg cttacctgga aaatgtcatt ttagcaaacg ttaaaaaaaa
    18541 ctggaaatat gcgatttaat cgcgtttgtc tcttccaagc agcttaaatt tattatgtta
    18601 ttcttaataa ttggtctatt atctgttatt tttctttcgt tgtttttgtc ttttaaattt
    18661 tcgataacac caataaccga aaagcgttag cactagcaat atacctagaa ggagtataag
    18721 taatcgataa agatggtatt tttttgtctg ttctatcttt gaagagactt cttctgtaaa
    18781 gggaactcgt cttcctgtca ctagtaatcg gtgagtgttc acggtgtaag gtgtacaagt
    18841 gataagtgtg actagatcct gcttttctct aattgctaat gtatttgttt ttgttggaag
    18901 gacaacaatt ttctcaacga cttcataagc gagcatccga ccatacactt caagatagaa
    18961 tcggtcatct tgctccattt cttcaagatc cgtaaagagt tttttctcag caataccgct
    19021 atgggcggta atcacggaat gtgtactctc tcctccagtt ggaaaagagg tgccttgcag
    19081 gactgtcgct cctctatcta acaaaagatc attcgtttca tcaaataaag gcaagctaac
    19141 attgattttc ggaatataga tggctccgat catatgttgt tcgtaatatt cttttcctgg
    19201 attttggaca tttctaaccg cttgctcaaa aggatctttg acttgtccca ttcctgggat
    19261 tactgatgta tgagcaagcg tctgattttt cttttcatgc actgctaatt gttgctcatg
    19321 tttttcttct ttatcgcgtt caagctgctt ttgataattc tcgatccttc tttgatccaa
    19381 ataattgctg agagcatctg ctaaaaatgg atatgtaaaa actgcagctc cggcaagaaa
    19441 catcactgcc ataaataatt gtaataaaag actgatttgg gtttgaggtc tattacctat
    19501 tttctttttc attttcttca ccttcttatt taaaaaaact gcagcaacct ttataaaacc
    19561 gattgctgca gcttattttt gttgcttata cttcagactt cattttagat ttacggtacc
    19621 agctgtacgc tccaatcatc aaacttattc cgattagtaa gaatgccaag agcccgttcc
    19681 caccagtagc aggaagtgac ccgcgtttca tattcggtac tctttctggc cctggatttt
    19741 ctactcctac gatctgaatt tcttcattat agcttccata agagatgata aattcagtaa
    19801 aggctgcctc acctgttgga agtacataac cttctggcgc catggtctct ttcatttgat
    19861 aggttccata atctaaccct tgaacctcaa agtatccatt tccatctgcg acaaattctg
    19921 ttgcttgtcc gcttgttgcc cactcaattc tatctgctcc tgattcataa acagctaaag
    19981 gcgactcccc tttataaaat attgcgtatt cagaacggtc ttcattccac aattcgaatc
    20041 ttgcatcttt aagcaattca ctgcttgatg catcatattt ttcaaacttt cttccacctg
    20101 ttgtgacttt ctctggcggc tctactcctt cttcataatc aaattcatca tttcgtccga
    20161 tttcaataac cgctgtgttg ttatggaaat catccgcttc tagttctgtg gaaaccttca
    20221 aattatacgt gatcgttaaa tctcctccac gtgcatctgc taatctacct aaagtatcta
    20281 ctttggctac tccattttct tctgtcaaaa tcgtcacgac aaacccgttg cttactactt
    20341 ctactatata atcttgtcct tttgttaaaa tcgtctcgcc ggctcgcact tctatttcct
    20401 gatcataata agttagacca actgtaggcg tatctgtgat tttgtaattt ttgataacag
    20461 cgcttccgtt ttcaaggaca gaatcgatga aataaggaat aggtgctgtg atcgtgtaag
    20521 aagccgtttt tcctctttct agatcaatat aagaaatcgt ttcttgttct ccagcttcat
    20581 taataatcgt tacttgtcta aggtcgcttt cctctacatt catctgttta tctgcttctc
    20641 tgatttcgtt tttcggatac aaatgcacat ctgtattgta tgtgtctttc catgtagaac
    20701 catcccacac gccatccacc atatcaggca tcatcactgg catcataaca accattggag
    20761 acgcgattct ggaaatatga gctggcgaat aagtttctaa gaaaagatag actttatctc
    20821 tagaagaagg tgcttcttcc gtcacttgga cggtagagaa taccgcttct ccagcttcat
    20881 tcgttgtttg tttgtcaata cttgtcagtc gatattttaa aaaccaactt tctgtgtgcc
    20941 gtttttgaat ccattcaatc gcttcggcag aattaagacc gtcttctcgt tccggtgtta
    21001 aaggattatc tcgaatcaaa tcgtaatata cttccgttac gtcaaacaaa tcaaaatcga
    21061 ctcctggaat attttcttca ccaaaatccg tagaaaccaa tccgctattc ggtcgttctt
    21121 ccggaacaga tgagaaacct tttttgtgta atgtaatcgt cacttcactt ggtttttgtg
    21181 cgacttcttc ggctttgact agttttccgt tcccgaaaat ccctattccc gcagtaacga
    21241 gtaacagtac actcattccc gtgacaatca tttttttatt catttttttc tccccgttct
    21301 ttttttatta ataaaacagc acctaacaat aaaagaattc ctagtaaaga aatcaacgtt
    21361 cttgcttcat tcgtttgcgg gaatacggac cattttcctg attgacctgg attgcttgga
    21421 ccactcggct ctttcggttt gtccgttgtt tcttttacgg tctcgttata aacacgtggt
    21481 gttgcggatt cataggcaga gactggaact ttcccggatt ctaattcagc catctcctgt
    21541 ccgttaacta ctacatattg tggttggcct tttgcatcca cccaaaattc agggatgatg
    21601 acctcgattc tcttacttgc ttcatccatc tcgtaacctt ccacaccttt taattcttca
    21661 aaataatagg tgccagaagg caagaaacgt tgtcctgtcg taacaaggcc ctctttatcc
    21721 gaaatgaatt tgcttacatt tttgtcattc aaaggatcat tgtctgctgg tttgatccat
    21781 tgatttttca agtcattagc aggagccata tctaaataat atttcccccc ttctatcatc
    21841 tgatatagag caaaaacagc accttctaat ggttttcctt tttcagttgt tcctctttga
    21901 cgtccatact tgaagaagta tggatctctt agataaccga catttttagg atagatatga
    21961 atctcttgca gttcctcggt tgattctgtc gggtgataaa ttggcaaaat agcagcaatt
    22021 gggatagctg tctgatcgat atcaacatcc aatccaactt ctggatcaat cgattcttcg
    22081 aaaatcaaat aaactgcatc ccgatcttgg tttttcttag gtgctttgat acgtgcaatc
    22141 ccgtctccta atccgccaac cgtatctttc ccagttgtta ccgaagtccc tatttggaca
    22201 agcctttctt ctgtgattag ctgacggata ttgtttctgt tcgtttcagc gatttgctgg
    22261 acgaattctt taggagtcat gttttcagca gctttttttt catgataata atcggtcatg
    22321 tcgtaaatgg caaatgtcgc tccatttagt ggcaccgtct gtgaaatgat atctgctgtt
    22381 tcccctgctt cctcagagat agccaagcca tcattttcat ggtattcaaa ttgatcgtta
    22441 taatcgatat ctcgaatcat ccgtttatgc aacacgaatt ccacgacatc ttcccctgat
    22501 tcttctgccg acatcgcatg tggaagaagg actgctgcaa gcggtagtaa aacaagtaat
    22561 tgaagcatca aacacaacag ctttttcatt accttgctcc ctccttttta gattgtctga
    22621 caaacaaacc ataggctgct cctgcaagcg ccatgaagaa tgcagcaagc aggatataga
    22681 ataatcgtcc ggatcctcct gtagcaggca acggattttc aggatcgttc ccgacactaa
    22741 gctgtaatct gattcgattt gtctccaaaa gttcatattc taactcttgc tgtagatcag
    22801 gcgtctctgc ttcttcgttt ggataatacg aaatctcaac ttttagtgta cccgcttctt
    22861 ttcctggaac tacccgatca ttttctatgt caggttcttc tcgataacta atttctaaaa
    22921 taaagtaacc tggaagtttt ctatagctgt caggtgactt cacttccttg atccgataga
    22981 ccccttccaa caagtctgaa gcattgaata gggaagaatc gtttggatct ggtgccaagc
    23041 ctccctcgat gatttcatac tctcctgcac tattttgttt ctcaattgaa aattctgcgc
    23101 cttcaagacg tcggctattc atactgtcaa ttttcaaaag ttccaaatcg attggcgcta
    23161 gttcatcttc taaggttact tgtagtacac catcgatgat ttcatagcct tccggcaagg
    23221 gagcagtttc gtcttcgatg accgtatctt caatgataaa cttaccttct tcattcagtg
    23281 caaactggaa cacttgatca gatagccgat acccatttgg tgcgacagat tccgagacct
    23341 catattcacc aatagacaga tgcagaggtt ggtcgtaatt taaatcgaca tacttttcta
    23401 ctcctacggc acgagaatag aagtatccga tcccaggcaa acgtttcgat tcttctggtt
    23461 ctaacggatt accagtgtat gctcgataca gcgggaagga agaatccccc actttcctga
    23521 tatcgaaacc tgcaccatct aatgtatggc cttcatggtc taattttcta actcggatct
    23581 cccgataatc aaacgtattg taagcagtga atgctaccca taaacgatcc tcttcatctg
    23641 ttgtatacgt gagatattgc caattttctt cacctgtttg cttgagttgg atagtcccta
    23701 tagcattatc gtaaagtcgc aagatccatg tgtcattttc atcaggttct ttgaaacctt
    23761 ctgggttctg gctctcaatg attttgtatt ctgcatagtt ggtagtttct tcactatcct
    23821 tataaacaaa tgaatctggc tctccgattg gatgaataac tttctcacca tccacgatta
    23881 catatttccc gtcttcatct actgaataga acgtgagtct ccctttctct tcctctactt
    23941 cgttcacacc agaaagccct ttcgctactg gatatccgat ttccagtccg ttaccatctg
    24001 tttcataaag ttcaaactct gcgccattga ttggcatatt actgccttca attagtttat
    24061 tgatattcat gtggattggc ttgaattcat ttcgcatttc cagtttgaca tacaactcat
    24121 catactctgt gccttctatg ttaggctctt gtacaacaga aagcttgtga tattctcctt
    24181 cataatagga attcggatct ttaccttccg gtccgccgac tctcgaacca gatgtatatt
    24241 catctacaag ctcgtagtga cctaacatct catcttcgtt ggctgcatat tttttgattt
    24301 ggaattgata cgtctcatct actaactgat aacgtggtgg tccagcgatc tctctgatcc
    24361 aaaatatgtc gctgactgct ccagtttcat ctcctaaaat cggcatcttc gtttcaaaga
    24421 tcgctattcc gtccgtggta gttttacttg tttcgattgg ttcggcttcc ccactctctg
    24481 gattttggtc atataatgca aattcgactc cagccaacgg ctcgccattt tgacccttct
    24541 tgctgacttg gaaattacgg cctttatgtt cgtaacgaat ggaagaagat gggaaataca
    24601 tccgtccagt agtgatatca ttctcataat cctccaggtt ctgaatatag gccatattgt
    24661 tcactgtgca aaaatttccg tcttgagctc ccgcatcaag ttttacttgg tacttgatcc
    24721 tgaccatttc cccttcacct aaagaaatcg gagaaaattt ataagcttgg ttttcccgtc
    24781 cgacatacgt tgtcggagtc gccactactt ctggtggggc ttgtccatga cgttcttcat
    24841 cccatatttc tggctcttgg tacttcatgc cgttttctcc tggatctaga tggtaaaact
    24901 gaatatctgc cgatctgcca gctccaaatg gtgagacatt aaaaccagtt tcatcataaa
    24961 gtgtggcttg ctggatcgtt ctcttgaagg tagaagagat atcctccaag gctgtaacaa
    25021 tgtcattctt gttgttcgca ttgtaatatc gcggtgttac gccatcttct tctaaatcag
    25081 caatattttt caatacattt cgtccaatag catctagtcg ttgtctcccg ctaacgtttc
    25141 catcgattcc taaaccaatg gagaagatac ttggcgggtt ctcttgatac ttgtttttca
    25201 gccaatgatg ataagcaact gtgttgatcg cagatgactt ttcttgcgaa gtaggactgc
    25261 cattcgtatt gttattagaa gaaccatcac caacccagtg agtcgcttta ttatctcctt
    25321 cttttacctc tccataacga tagtgtacat ttacttcatc ttctggacgg ttaaagtcag
    25381 atgcataggt aactggatga gtaaaccctg cattagaagt attcccagaa aactcctcaa
    25441 aatttctgaa caaaacgtca ttatcttcag caatcttatc ttccatcaca gtccagttgc
    25501 tccaagctcc attagtgctc gtacgataac ttgactgaat cggtgcataa ctgaaggtcg
    25561 gtgtcccatc tcccacaacg attaggattt tttctggatt tctattttct ccgccattgt
    25621 ctttatacaa tgtttcatag ccatttttaa gccctagtgt aagcggagtt cctccgattg
    25681 gtgtacgatt taatgctgta ttctcacttc ccgtcaaacg attgatatca tttgtcaatg
    25741 gaaagatgtc cgaactgatt tgctcatggt tattgatagc tgtactatta tggtaaaaat
    25801 tcaccatacc gattcgaata tccagactcg ggtcagagag cagatcttta ctcatttcgt
    25861 ttactgcctc aatcaacgca gcatttttag tttgactatt ggtcccagcg gttaattcac
    25921 tcatactcgc tgacttatct agaacaaaaa cgatatcgat tggactgatt tcctttttct
    25981 cgcccccgat gacatctaga tagacattgt aaagggttgt tgcatcttca ggatgtagat
    26041 cagatgctgt gctgcttggc agaacaggtt ctacccattt tttgatgtac gccccgttat
    26101 aatccaagta actgttttca aaattccgtt catttcccca aagctgaacg gtttgagctg
    26161 gaacgtttct atcttcttcg cctgcagttg atccattatt agtagcatag tcgtaatttc
    26221 gccagttcgc tcccccgttt gtataatcac tatcatcgat caagttctct ccgtcatatg
    26281 gcgagccttt gtctccttga tagcgttcat tgctttgtgt ccaatttcct aacacttttg
    26341 tatagtcatt cgttgtaaac ttcatctatt ctttcatcaa ccaccacttg aagaggtagt
    26401 tacccttcgt tcacatcggg gatcttttct gtttcaaaaa tcaatgtgcc ggtttctgct
    26461 tcttttgaat aaacttttcc gacataccat tcttgaccat cgatcgtctc gacttttagg
    26521 tcttctccat cttcatcata ggaggataga cctgttccac gaacgttttt tacggtacca
    26581 atcccggcgg ctgccgcatc gattgctaaa cgtattctac cttcattggc actttccttt
    26641 ttttcaaaag ccagatgcca ttcaagggtt tcttcttttt caatatactt cccttcaatt
    26701 tttgcatagt cctcatctaa gaatacctca tcctcctttg tttctgttga ctcagcagct
    26761 acatccaggg tcgtcatatg atagactgac tggaaatatg gtccaaatag caaactgaca
    26821 gttactaact gcaaaagctt caattttaac cctttcacat ccttatactc ctttcaaaaa
    26881 tatttgtctt ctttcac
//
LOCUS       DAHHZJ010000022        26761 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.023, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000022 DAHHZJ010000000
VERSION     DAHHZJ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.023"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(<1..1530)
                     /locus_tag="NBL02_000756"
     CDS             complement(<1..1530)
                     /locus_tag="NBL02_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571463.1"
                     /translation="MKIIKKKIAGRKINWLALSLLALMFIVIFTGNVAHASIFNWFDD
                     TESQTEFLNNPVYQPYLKKYNGNFYSQFGLWIGWSVIKSMFAIVDGIQNMIPEVLDLF
                     SFVESAGLNKVYQSVLSTIVVGLMILSLVIIGYKMVTGKGTVDLKSVGINVLMSIVLI
                     LLMPTLIGSTEKLSGIMFAKTLYNDATSINNQDGGVAWSLIEQGVTDLAYINKTDQYS
                     MIDKTNDRNNLTKENFWKTDLTQVLTDNVIDKMEKENSTADNLRYELVENSDNEFVAT
                     KFSDNFLSTFVDGIKSGYYRYQANLWGISIGLIALAIAYVFSAFVIITAILELAFKRV
                     LGVLVFATDLETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIF
                     IKTIAYICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRA
                     KGSGNGNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMAS
                     ER"
     gene            complement(1517..1735)
                     /locus_tag="NBL02_000757"
     CDS             complement(1517..1735)
                     /locus_tag="NBL02_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571464.1"
                     /translation="MIENRLIDQICCLDQNVDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQEMAQKLSESLKKWAEGGNNENN"
     gene            complement(1728..2693)
                     /locus_tag="NBL02_000758"
     CDS             complement(1728..2693)
                     /locus_tag="NBL02_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HCD2571465.1"
                     /translation="MKTLFDYILTLTGGSVEFMAEKRMLSKTFLNNASFMKMSDSAKL
                     LYVYLNVNADDDGIVEAYPYVRMLGTPDDDVKILLAKEFLYMLNEDLLVFLPHWLQNQ
                     TLRTTRYTPSIYRFKLLNSNPDFFTRLKQISIEKILKAGQTEMLRNDLIEKWYPVGLP
                     KDDHGHAQVRLGQVSSDQSRSGKVSLGEIREDQSINLSDDMEVEIMRQKIKNQIGYNA
                     LKSNPCYKRIVEDIVNIIQSIYSEKSKTYRINREEKSANEVIEKVEQLNQFDIESAID
                     SFNAIDHEIHNLNLYWITTLYNCKNTVSSRAYNNFVTQTREVLYD"
     gene            complement(2883..3167)
                     /locus_tag="NBL02_000759"
     CDS             complement(2883..3167)
                     /locus_tag="NBL02_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD2571466.1"
                     /translation="MDVSSVQVILPPDLDQSLRKYFYQVAKEAVDQVRIETSLSKEIF
                     RLGEACEYLSCSRSTLKKFINQRGLKVSIIGDCQFIRKEELARFIKEYEI"
     gene            complement(3180..3473)
                     /locus_tag="NBL02_000760"
     CDS             complement(3180..3473)
                     /locus_tag="NBL02_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD2571467.1"
                     /translation="MTDNEINAILSLVKTKKDEVVRLDSPVRKYRRFCGYRQKELAEL
                     LEISELTYRNKEKGRVPFKDYEIERIVKLFHKDGIHLDIVDIFFSDLKTKKDE"
     gene            3542..4306
                     /locus_tag="NBL02_000761"
     CDS             3542..4306
                     /locus_tag="NBL02_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD2571468.1"
                     /translation="MNLNKTSVGNRIRLVRKELGLSMKEFGKKITPTAADSIVSRWER
                     GISLPNNDRLTQIAKIAHKDINWLLWGTFKEFISSLLDKEGYTNFLNDFPETVDTIYN
                     DLMPISENLQFPDYENSILRLFNKIYLPIFNDYCLDIIELDLEDFINKRHSISSDKFK
                     NRFLSSFHEGVSNSGYKYGDTDLIYKYGIALLEKFDDSYKLNNPYESIDAYFSAMTVN
                     TEDTEKLIYTICKSLGIPFQKGSNTAQILSKNHNNF"
     gene            4357..5502
                     /locus_tag="NBL02_000762"
     CDS             4357..5502
                     /locus_tag="NBL02_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCD2571469.1"
                     /translation="MATIKQYEKKDGTKAWLFQTYLGISPLTGKEVRTTRRNFKTKKE
                     AQIELNRLLVDFEENGLKKETNETFKEVYDLWYESYKTTVKEVTQLKIEIKFNKWILP
                     KYGQFRIKEITTKHVQKILNQWAKTTDQYKVLHSTASRIFKYAMTLGIITKNPCELVI
                     MPNRNVEKKKDTVKIYSRDQLESLFQYLESKRSTYRADYDKTLLRFLFFSGCRISEAL
                     ALDWSDIDFDSNTVTINKTLSQTKYGYKISSPKTRNSYGSLSLDNKTINWLKKWQINQ
                     KRYMLTLGITDPTMIFCGIYKEIITKHAIYSRMITICKNSGIPFLGNHVTRHTHASML
                     LESGVSMKEVQERLRHSSIKLTMDTYAHLTKETKEKTVEKLAEHLNF"
     gene            complement(5587..5892)
                     /locus_tag="NBL02_000763"
     CDS             complement(5587..5892)
                     /locus_tag="NBL02_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HCD2571470.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(5918..6349)
                     /gene="ruvX"
                     /locus_tag="NBL02_000764"
     CDS             complement(5918..6349)
                     /gene="ruvX"
                     /locus_tag="NBL02_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCD2571471.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(6346..6612)
                     /locus_tag="NBL02_000765"
     CDS             complement(6346..6612)
                     /locus_tag="NBL02_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HCD2571472.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6866..7591)
                     /locus_tag="NBL02_000766"
     CDS             complement(6866..7591)
                     /locus_tag="NBL02_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HCD2571473.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7957..8027)
                     /locus_tag="NBL02_000767"
     tRNA            complement(7957..8027)
                     /locus_tag="NBL02_000767"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7993..7995),aa:Gly,seq:ccc)
     gene            8144..8896
                     /locus_tag="NBL02_000768"
     CDS             8144..8896
                     /locus_tag="NBL02_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCD2571474.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8912..9391
                     /locus_tag="NBL02_000769"
     CDS             8912..9391
                     /locus_tag="NBL02_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCD2571475.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(9642..11222)
                     /locus_tag="NBL02_000770"
     CDS             complement(9642..11222)
                     /locus_tag="NBL02_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HCD2571476.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(11215..11958)
                     /locus_tag="NBL02_000771"
     CDS             complement(11215..11958)
                     /locus_tag="NBL02_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2571477.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(12134..12910)
                     /locus_tag="NBL02_000772"
     CDS             complement(12134..12910)
                     /locus_tag="NBL02_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD2571478.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(13158..13994)
                     /locus_tag="NBL02_000773"
     CDS             complement(13158..13994)
                     /locus_tag="NBL02_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HCD2571479.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(14005..14934)
                     /locus_tag="NBL02_000774"
     CDS             complement(14005..14934)
                     /locus_tag="NBL02_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCD2571480.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            15126..15479
                     /locus_tag="NBL02_000775"
     CDS             15126..15479
                     /locus_tag="NBL02_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCD2571481.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(15553..16209)
                     /locus_tag="NBL02_000776"
     CDS             complement(15553..16209)
                     /locus_tag="NBL02_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HCD2571482.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            16435..18369
                     /locus_tag="NBL02_000777"
     CDS             16435..18369
                     /locus_tag="NBL02_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCD2571483.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(18455..19678)
                     /locus_tag="NBL02_000778"
     CDS             complement(18455..19678)
                     /locus_tag="NBL02_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HCD2571484.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(19672..21570)
                     /locus_tag="NBL02_000779"
     CDS             complement(19672..21570)
                     /locus_tag="NBL02_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HCD2571485.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(21564..22682)
                     /locus_tag="NBL02_000780"
     CDS             complement(21564..22682)
                     /locus_tag="NBL02_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HCD2571486.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(22765..23598)
                     /locus_tag="NBL02_000781"
     CDS             complement(22765..23598)
                     /locus_tag="NBL02_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCD2571487.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(23591..24508)
                     /locus_tag="NBL02_000782"
     CDS             complement(23591..24508)
                     /locus_tag="NBL02_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCD2571488.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(24735..25859)
                     /locus_tag="NBL02_000783"
     CDS             complement(24735..25859)
                     /locus_tag="NBL02_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCD2571489.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            26042..>26761
                     /locus_tag="NBL02_000784"
     CDS             26042..>26761
                     /locus_tag="NBL02_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD2571490.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVN"
BASE COUNT         8436 a         5283 c         4277 g         8765 t
ORIGIN      
        1 tctttcacta gccattgtag ctccatctga agctaaacct gataatccac gttcatgggc
       61 atatcccaaa gctcttccag ttttgccagc tttttttgat agagtttcgc cgaaattctt
      121 ttctggtttt cgatcttctc cttctccgct gtctgatcca tttccatttc cgcttccttt
      181 agcacgatta aatccatttg atccttttct aaacagcatt gcgcccatac caaaagcaga
      241 agcaagttgc ccataacctt ctttgagtcc aatatcaaca ccaaagtatc tcatgactgt
      301 tccactgcct ttgattaaaa caaaaacggc acagatataa gcaattgttt ttataaaaat
      361 atttgttgag ataccttgtc ctgagttcag aaaattgata aacatagcaa acatggatag
      421 tccaaatcct tgaaagccta cagtcaaata gcactgtaga atatctgata gaacaacttt
      481 tgaacgctgt cctgtttcta agtcagtagc aaatactaaa actccgagta cacgtttaaa
      541 tgctaactcg agtattgcag taataatcac aaaggcgcta aaaacatagg ctattgctaa
      601 agctatcagc ccaattgaaa tgccccatag attcgcttga tagcgataat atcctgattt
      661 gataccatca acaaaggttg acaaaaaatt atctgaaaat ttagtagcaa caaattcatt
      721 atcagaattt tcaactaatt cataacgcag gttatcagct gttgagttct ctttttccat
      781 tttatcaatt acattatctg tcaatacttg cgtaagatcc gttttccaaa aattttcttt
      841 tgttagattg ttccgatcat tcgttttatc gatcatggaa tattgatccg ttttattgat
      901 ataagctaaa tcagtaaccc cttgttcaat cagcgaccat gccacaccgc catcttgatt
      961 gttaatacta gttgcgtcat tataaagagt ctttgcaaac attattcctg aaagtttttc
     1021 tgttgatcca atcaaagtcg gcattaaaag aattaatact atcgacatta aaacattgat
     1081 tccaacagat tttagatcaa ccgttccttt tccagtcacc attttataac caataatcac
     1141 tagtgaaagt atcattaagc caacaacaat agtgcttaaa actgactgat agactttgtt
     1201 caatccagca ctttccacaa agctaaacaa atctaacact tcaggaatca tattttgaat
     1261 accatctaca attgcaaaca tcgattttat aacagaccac cctatccaca atccaaattg
     1321 actgtaaaaa tttccgttat atttttttaa atacggttga tagacaggat tattcaaaaa
     1381 ttcagtttga ctttccgtat catcgaacca attaaaaatg cttgcatgag caacattacc
     1441 ggtgaaaata acgataaaca ttaaagcaag taagctcaga gctaaccagt ttattttcct
     1501 accagcaatc ttttttttaa ttattttcat tgttacctcc ctcagcccat ttttttaggc
     1561 tctcagaaag cttctgagcc atttcttgat taattatgat atgagttaca caattgtcat
     1621 tttcagctct taaatcgaga accacggtgt taaagcagat actctcgatt aattcgagag
     1681 tatctacatt ttgatcgaga cagcagattt ggtctattag acgattttca atcatataac
     1741 acctctcttg tttgagtaac aaagttatta tatgctctgg aacttacagt atttttgcag
     1801 ttatataaag ttgtaatcca gtaaagattg agattgtgta tctcatgatc aattgcgtta
     1861 aatgaatcaa tagcagattc aatatcaaat tgattgagtt gttctacttt ttctatcact
     1921 tcatttgcag atttttcttc tctatttatc ctgtatgttt tgctcttttc agagtatatt
     1981 gattgaatta tattaacaat atcttcaaca attcttttgt aacaaggatt ggattttagc
     2041 gcattatatc caatttgatt ctttattttt tgtctcataa tttcgacttc catgtcatcc
     2101 gatagattga tagattgatc ttccctaatc tcacctaaac taaccttacc tgacctactc
     2161 tgatctgaac taacctgacc taatctaacc tgggcatgcc catggtcgtc ctttggtaga
     2221 ccaacgggat accatttttc aatcaaatca tttcgaagca tttccgtttg acctgctttt
     2281 aaaatttttt caatactaat ttgttttaat cttgtaaaaa agtctggatt actatttaat
     2341 aatttaaatc gatatataga gggtgtatat cttgtagttc ttaacgtttg attttgcagc
     2401 caatggggaa gaaatactaa taaatcttca ttcagcatat agagaaattc tttagctaaa
     2461 agaattttca cgtcatcatc aggggttcca agcatccgta catatggata tgcttcgaca
     2521 atcccatcat cgtctgcgtt tacattcaga tatacatata ataattttgc tgaatccgac
     2581 atcttcataa aactcgcatt atttaaaaaa gtttttgaca acatacgttt ttctgccata
     2641 aattccacac ttcctcctgt caatgttaaa atgtagtcaa ataaagtttt cacagaactt
     2701 tattattgag tgttcttccg gtctgcaaac tgtaaggagc actctttttt tgtatattca
     2761 ccttttatta aatcaaatag aaaataaatc ttgatttctt ttaaactcaa ctttgcttct
     2821 tcatcatcgt tcaaacaaac acttcctttt tttcatatca aaagcacacc acgcaacttt
     2881 ttttatattt catattcttt gataaatctt gctaactctt cttttctgat aaattgacag
     2941 tctccgatga tactcacctt taaaccacgc tgattaatga atttttttag tgtacttcta
     3001 ctacaactca aatattcaca cgcttctcca agccggaaaa tttctttgct taaagatgtc
     3061 tcaatacgaa cttgatcaac agcttcctta gcaacttgat aaaaatattt acgtaatgat
     3121 tgatcaagat ctggcggtag aataacttgt acactcgata catccatgat gcccactcct
     3181 cattcgtctt ttttcgtctt caaatcacta aaaaaaatat ccacaatatc taaatgaatt
     3241 ccatctttat gaaatagctt tacgattctt tctatttcat aatctttaaa cggcaccctt
     3301 cctttctctt tgttacgata cgttagttcc gatatttcta ataactccgc aagttctttt
     3361 tgtctatatc cacagaacct gcggtatttt ctaactggag agtccaacct taccacctcg
     3421 tcttttttcg tctttaccaa cgatagaata gcatttattt cattgtccgt caatacttac
     3481 ccgtcgtttt tcgtagattc cattccgttt ttgtgttaca ttactgtcgg agggatataa
     3541 tatgaacctt aataagacat cagtaggaaa ccgtattcgt ttagttcgta aagaactcgg
     3601 gctttccatg aaagagttcg gaaaaaagat aactcctacc gccgcagata gtattgtttc
     3661 gcgttgggag cgagggatta gcttacctaa taacgacagg ctaactcaaa ttgctaaaat
     3721 tgctcataaa gatattaact ggttattatg gggaactttt aaagaattta tttcatcatt
     3781 gttagataaa gaaggataca caaatttttt gaatgatttt cctgaaacag tagatactat
     3841 ttataatgat ttaatgccta tttcggaaaa tttacagttt cctgattacg aaaattctat
     3901 tttacgacta ttcaataaga tatacttacc tatattcaat gattactgtc tagacattat
     3961 tgaactggat ttggaggact ttataaataa gagacattcc atctcctctg acaagtttaa
     4021 aaataggttt ttatcctcct ttcatgaagg agtatctaac agcggatata aatacggcga
     4081 tacggattta atttataaat atggaattgc attacttgag aaatttgacg attcttataa
     4141 attaaataat ccatatgagt caatagatgc gtatttttct gccatgactg taaacactga
     4201 agataccgaa aaacttatat ataccatttg taaatcgctt ggcattccat tccaaaaagg
     4261 aagcaatacc gcacagattc tttcaaaaaa ccacaataat ttttaattca ttttttctat
     4321 caaaagcacg ccacgcaact gataggagga aaaacaatgg caacaattaa gcaatatgaa
     4381 aagaaagacg gaaccaaagc ttggctattt cagacctacc ttggaatcag tccattaact
     4441 ggtaaagaag tccgtactac acgtagaaat tttaaaacaa aaaaagaagc tcaaatcgag
     4501 cttaatagac tacttgtaga ctttgaagag aacggactta aaaaggaaac aaacgaaact
     4561 ttcaaagaag tgtatgatct ctggtatgag agctataaaa ctactgtaaa ggaagtcact
     4621 caattaaaaa ttgagattaa gtttaataaa tggattcttc caaaatatgg gcagtttaga
     4681 attaaagaaa ttactacaaa acatgttcag aagatattga atcagtgggc taaaacaaca
     4741 gatcaatata aagtcctaca ttctactgca agccgtattt tcaaatatgc aatgacttta
     4801 gggataatta ctaaaaaccc ctgtgaactt gtaatcatgc ccaatagaaa cgttgaaaaa
     4861 aagaaagata ctgtcaaaat ttattcacgg gatcaactag aaagtctctt ccaatacttg
     4921 gaatcaaaaa gaagcaccta tagagccgac tacgataaaa cgctacttcg ctttctattt
     4981 tttagtggtt gtcgaattag cgaagcttta gctttagatt ggtcagatat tgactttgat
     5041 tcaaacaccg tcactataaa caaaacctta agccaaacaa aatatgggta caaaatttca
     5101 agtccaaaaa cgagaaatag ctatggttct ctatctttag acaataaaac cattaattgg
     5161 ctaaagaaat ggcaaatcaa tcaaaaaaga tatatgttaa cccttggtat tactgatcca
     5221 acaatgattt tttgtggaat ctacaaggaa atcattacta aacacgctat ttattctcgt
     5281 atgataacaa tctgcaagaa ttctggtatt ccatttctag gtaaccatgt aaccagacac
     5341 actcatgcta gtatgctgtt agagtcagga gtctccatga aggaagttca agaacgcctt
     5401 agacactcat caatcaaatt aacaatggat acctatgctc atctaactaa agaaaccaaa
     5461 gaaaaaaccg ttgaaaagct tgcagagcac ctaaatttct agcggaacgt attcaatctc
     5521 gtattcaatt caaaatacaa aaaagctgaa cccttatcta ccaagggttc agctaccatt
     5581 taaagcttat tcttcttctt ccgccatgaa tgtattgaat acttcttcga tcatatccca
     5641 ttctgcgtct gtttcgattt gttctaactc gccttctgtt ccatcttcgt tttcgatgta
     5701 tgcataagct tgtaattcta catcttcatc ttctgaaaca ccagctggat ataaaagaac
     5761 atagttacgt ccaaattctt cttgtccgtc aactgttagc aagatttcgt ataatgtttc
     5821 atttccttgg tcatccacca atgtgatgtg ttcgtgtcct tcgtgttcgt gggaatggtt
     5881 atgttctgtc ataagattac ctcttttctt tttattatta aaataattgc ttacctgagc
     5941 cgtctaaata attttgtaaa atcatcacag ctgctaattt atcgatgact ttttttcgtt
     6001 tcgctcttga agtatttgcc tgctctacta gcatacgttc tgcctgaaca gtggttagtc
     6061 gttcatcttg ataatcaacc ggcaaggaga atagttcttt gatcttctct ccatatgaca
     6121 tggaagcttc tgcccttggt ccgatggaat tgttcatatt cttaggtaat ccaactacaa
     6181 aacggtctac ttcgtactgc gccactaatt ccttcaaccg atcaaaacca aactcttctt
     6241 ctgcctcatt gattcgaatg atttctactc cttgagctgt ccagccaaag ggatcgctca
     6301 cagcaacgcc tactgtctta gaaccgacat ccaagcccat tactctcata aattcactcc
     6361 atgatttgag aggtagtctt tgacaattac ttccatgatt tcatcgcgtt cgtgacggcg
     6421 aatcaaattt cgtgcatcac gataacgagg aatgtaggcc gggtctccag aaagcaagta
     6481 gccgacaatc tgattgattg gattgtagcc tttttcttcc aaagcccgat aaacaatcgc
     6541 taatgtttcg ctcacttctt ttttgcgact atcgtcaaaa ccaaaacgta cggtttcatc
     6601 tgtaaaaccc atgattctac acctctttcc cgcaaggtaa ttccagctta ccttctacac
     6661 ccttatttta ctcgaagaaa gggaaaacta caaacaaata ttccaaactt ctgaataaat
     6721 taagaaacac gatttgtctg atttttcctt ttttctgtgg ctttttcaaa agccctcttt
     6781 ccataaagta tacgctatat ttctttgaaa gtatgttttt acacaatttt ttcataaagt
     6841 tcataaaaaa ttcgaattgc tgctactaat ttttcgtttt cgttgcttgc cctgtctcat
     6901 aattgatggt gtagccatct tgaacattgt agataaagac attgaattca atttttttgt
     6961 cttcaatgct ttctgcttcc aactgcacac ctcttgctac cagttcattt ccctcgaaat
     7021 gaggagtgac acgatagcgt acatgatgat ttgtttcctt gatatatgcc gcaatatcat
     7081 tttcgtgggc aagcatgtac ggagcattca acgaacgtgt ccctgtcatt aagtttttaa
     7141 tgttattatt ttgaccagtc aactggaaac caatcaaatg gctgcggttg tataaccatt
     7201 gtccgtcact taatttcttt tgtttccaac ctgtcggatc aatatacaaa ggttctctct
     7261 tttcagtagg aaacaagtct tttcccaaca tcgcattggc ttgtcctact ctgttcaatt
     7321 tgtctatatt tgaatagctt tcccatgtcc ctttggaaag actaagatcc tcttttgtaa
     7381 actccggctg attcccattg atttctattt cttgatgttt tccatcatat tctggcacat
     7441 ccgctacttg agaagcttgc ggtgtggaat caaaaatggt tgtaatttgt tggaagatat
     7501 ctgaatcttt ttgtgtggtg gaaataccga gtgcccctgc aatcacagca gcgacgatcc
     7561 ccacgatcgc cttggttgtt tttttgctca tttctttact cctttactaa cagttcccta
     7621 aatcttaaca tatgttcccc tataaataaa tactggaaag aaatagaaac agtaaaaaaa
     7681 taaaaccagt ttttagacgg cttaagaata caaaaaaaga gacggattca tcgtctctac
     7741 tcaatcacaa aacactattt acaaggtatt tattatcatt caaaaaattt ttcttaatac
     7801 tcaaaactaa acggcttctg tacgacctct tggtaaacag tgttcaaaag cttgcatctg
     7861 cggtaataag tccaaccaag tcaaaaacat aagaagcttt tttgcttgac tattcttatt
     7921 acttgatgca gggcagcttt ttcacagcct cttttaagcg ggtgacggga atcgaacccg
     7981 cgacatcagc ttgggaagct gggattttac cactaaacta cacccgcgat acaaaaacag
     8041 tatacgcctc tttcactcct tcctcaagtt tttttattct ttacgcttcc gatatgtttt
     8101 gtagtaaaat aaaactaatc aatcaattat tggagaagat gctatgacgg gaaaagaatt
     8161 agatctgttg ctggacactt gtttactcgc tgggaaaatc atgatggaaa gcaatgcgga
     8221 gatgtatcgg gtagaagata cgatgagtcg aattgcttta gcttctggaa atttccgttt
     8281 ggtcagctat gtcactcaaa ccggtctctt tattggtctt gatcgtacct cgaccatccg
     8341 aatggaacaa attaccaacc gttcgattaa tttagaaaaa gtagtaaaag taaatgatct
     8401 ttcacgtaaa tatgttgctt cagagctaac attagaagaa ctttactgcg aactgcaagc
     8461 aatcgaccga gaccgttctt tctttcctgt ctggttgcaa atcgttagcg cggcagtgat
     8521 cagtggaaca attatggtgt tatttggcgg aaagatgacc gatttacctg tgactttagt
     8581 cattggagga ttgggatacg cagcttatct atatagtttg aaatttttta gaatcaaatt
     8641 tttatcggaa tttttatctt ctttattgat tggcatagcc gctatctaca gcgtacgttt
     8701 tggcttagga agcaatcagg atctaatcat tatcggatgt gtgatgcctt tagttcctgg
     8761 agttcaaatt acgaacgcta tccgtgattt gctagctgga cattatgtct caggagtttc
     8821 tcgcggaaca gaagcgatga tgactgctac gatgatcggt tttgcgattg cttttatttt
     8881 ccagcttttt tattaggaaa ggaacacaag tatggggacg atatttattc agttttcttt
     8941 tagtttttta gcgactgcgg catttgccgt tattacaaat gtacctaggc gttcattgat
     9001 ttgttgtggt ctttctggtg cttttggctg gatgatctac tggatctgtg ttcaaatagg
     9061 cggatctgct gcttttggtt cattgcttgg ttctttagga gtagcattca tcagcgatct
     9121 tttctctaaa cgtttgaaaa tgcccgtaac gattttcaat attcccggga tggttcctct
     9181 agtccctggt ggtttagctt atcaagcggt tcgaaatctc gttactgaat cctatcgaga
     9241 agcggctcat tatgcggtcc aagcaattat gattgctgga gcaatcgctt taggtcttgt
     9301 attatcagaa gtctttaacc ataacatccg taatttcaaa gtaaagcgtg acgcaatcat
     9361 gatgaaacga aaaaaacggc tttttaaata aacgaagtgt aacaaagaaa aggttgtgaa
     9421 agagacgttc agccttgagc gatgacaaaa tatctcacaa acattgcgac aagtaaccgc
     9481 aggagcaata agaagtaatt atgagtatta aaaaaagttt tgtaaaaata gcttctcaca
     9541 tttttacata aaattgactt aatactgaga aactgacttt tgaacaccct ctatctgtaa
     9601 aaaaggggct gcgacatgat ttttgtcacg gcccctcttc tttattcaaa ttttttccaa
     9661 aacgttcgtt gataataaac gatccataga gcggaaagta ttagaactaa accgatcaca
     9721 ggaatattca ctaaccaaaa tggataaaac aaagccaaca ttccgtataa gacgataaga
     9781 ataatactga tgatcaatga acccatcata gttgcgacta aacccaaact tcctgaacct
     9841 ctgttaaata attggttaat attggtccag ttcagcagca gcaaccgata atctctagcg
     9901 aaataacgta gtgacaataa gtagcagccg aataaagaac caaatagcaa gctagggata
     9961 aaaaacaaag gcagctgaaa agacagtccg cttaataatg cgatcccacc agttaaaaaa
    10021 ctctgcaata tccatccgaa tctgaacttt tcttttaaat attggttcat agagattggc
    10081 aaagaacgga taaacagaaa attttcttga tccaaagaaa taagattgct gataaaagaa
    10141 gtctgattaa ctgttaagaa agccaatgcg atccctacca ggaaaacaac accgattaac
    10201 ttattatcta aatcagttaa ttttatttcg cctcctaggg caaaagcaag aataaaaatg
    10261 attggagtta acaaggaaga agaaagaact tgcataatca aattaggttc ttttactaat
    10321 tgagcattgt aactaaataa aagctgatgc acattttggt tctttttatg ggttcttttt
    10381 attttgaact cactgtttga atcagtcagt tgctctgtaa gctttggtac tatcagctgt
    10441 tttacagcaa ggaaaaatcc cagagccgtg atagctagta ttccccaacc caataaacct
    10501 tgttgagaaa gtggctgact ggcagcatca tagaatggca ataatatcga aatcggatgc
    10561 cgatctgtta actcattttc aatagtcccc gtttgatgat tcatccacat gattccagca
    10621 aatgcgatca gcatcgagct gccaagcaag atactagaaa ctgtttttct atgtttttta
    10681 aacactgctg ttcgagccaa accaaaaaca atcaaactag ccagacaaaa aagaataatc
    10741 aaaaatagag aaaacaaaag gattgagata atgatagtaa gcaaaatccc tagatcagct
    10801 cgccatccgg ttaaaataaa catgactaga agcggaaaaa caaaaggaat gatggtcaaa
    10861 gcaactacca aaatctttgc tgaaaaaacc tgttcttgtc caaaaggcag aggcaaaaat
    10921 acttgcaggt catttccttc aaaaaagata ttataaatga ctgatatact ttgagttaaa
    10981 cctaacatac caaaaagtgc cacgtaataa gtaaagaaac ctggcaattt aacaaagttc
    11041 atagcaaaca tagcaaatcc gtaaatcagt aaaaaaatca gccctgaaaa aagattttga
    11101 gacaaaagat agatagttaa tcgctttccc ttttttcctt tctctcttgc tttattcgtg
    11161 agctgtggat taacatataa aagattaact cgaattaatt ctaataattg tcgcttatac
    11221 atacggtcca tctcctgtcg cttgccttcc agccattttc aaataaatct tttctagcgt
    11281 ttcatcaggt gagccattca ataattcact aactgccccc tgataaatca attcaccttt
    11341 cttcaaaata gccagtttat cgcataactg ttgtgcggta tccaatacat gtgtagaaaa
    11401 aatgactgtt tttccttttg cggcatgagc cttcatcatt tctttcaaat caaatgctgc
    11461 ttgaggatca agtccttgca aaggttcatc caatacccaa atatctggat ctggcagcaa
    11521 cgtgccgatc aaaatcgttt tttgacgcat tccatgtgaa aaacttgcga ttgtttcatt
    11581 ttgatgacta tacatatcaa aaagcgctgt caattctgct aaacgctttt cttttttttg
    11641 tgtgtctaat tcataggcag ctgaaatcaa gtcccaatat tctccagccg tcaattgtaa
    11701 aaaaatatct ggggaatcag gcacataacc gattttttgt ttgatactca atcgattttc
    11761 cgtcaatttc attccatcta ctaaaatcgt tcccgatgat ggttcaataa tactaactaa
    11821 acttttaatc gtagttgatt ttccggcacc attatgtcct aaaaatccaa aaatttcccc
    11881 ttgtcttatc gagagagaca agtcttttaa tgcttgtttt ttcccgtaac ttttacttac
    11941 attgatgaat tcaatcattt ctgtactcct tttgtttgtt attacttttt attgtaccct
    12001 tcgttattgg tggagtaaat gtcaaaaaga gtgcataaca aaaaaggata gaaacgacta
    12061 ggctgctact tgggttgttc tcttctacag gaaaacactc ttctaacagg taagatatga
    12121 gtctttgttt tctttaaaat gattcatttt ctatcttttc ttgcataaaa ttctttagtt
    12181 tatcaatact tctctttccc atctctagtt gttctccatt tgcaaatgtt actatttcgt
    12241 ttactctgct aatagatgta atgttttcag tattaatgat ataagctttt aaatctttac
    12301 ataaatactc atattcgagt gattttttat attttttaat tgtgccgttt acgagaatct
    12361 cttcatcttt caatactagg cctattttat ttttataacc tggcatgctc attagataaa
    12421 ggatttcatc gtaatggata tagtgatcgc tattaccata acggacagtt gtccaccttt
    12481 ttttcgtagc atgttgaaca ggatagtttt ctttttcttt caataagtaa ctttggatgc
    12541 tttttttatt tttttcataa gtgaattctt ttatcaaata accactaggc gtaatctttc
    12601 gattaataac agttattgct ttattcggat gagacgttag ataaacaatt ttacactttt
    12661 cattcttttc tctaagcatt tcgcctagtt cgataccgct ataataggcg ttcaaatcga
    12721 tgtctaggaa aaaaatatct gaggaataga tttcattatt tttaatgaat tcaacaaatt
    12781 gtataacgtt caatccctga acgatctcaa atttccaagg acttaataaa attctggatt
    12841 cttccaaaat ttctactagc attttttcaa aaaccaaact atcatccaaa ataaatatac
    12901 gcgttaccat aaaaagtacc tccactcgat attttactat caaaatcatt tcatacaact
    12961 tttcaacata catgttagga ataaattgta attctcttca caatcttttc caacaccttt
    13021 gaaaccgata tcaaatactt tcaggatgct gcttctaaaa taaatatcaa acaaatgaat
    13081 acatcatgca aaaaaagcca gacaaccgac gtccttgcgt gtcagctgtc tgagctatgt
    13141 ttatttattt tgttctttta aaaagaacga tacacatact gatttttcgg agcagagact
    13201 ttctcagcgt ttgtaacaac tacagatggg atttcacgct tgaatggttg ataatagtct
    13261 gattggatgg ttccttctat tttataccat tcatcatttt taaccgttac tccttccggc
    13321 atatgcgtca acaaaccaaa gactcctgaa tctgccacac aatggataat gccgaatcgg
    13381 aacaagaaga tatctgtatt gctatcgttc ggtgtttggt aaacaaatcc ttcatagctg
    13441 atttttttgc caatgaattc gctagggtaa ttataaatta gctccataac ttctaaatag
    13501 ttttcatctg tgatcttgat agtttgctta tccccatact tttctttcaa ctcattcatt
    13561 tggtctaagt agtctgattt gttgaaataa atactggtat ccggctttaa gtattgtgtc
    13621 tgcatctccg gatcccctac tgattcttta cttactggga aattaaatcc tttggcttct
    13681 acgatcgtag tgtcgagact aactgttgga aacaacgtcc ccacgattac tggcaaagct
    13741 agcagtacat aagcgattcc acgttgataa ggttttgaca acccatggtc atgatcgtgc
    13801 tcgtgtgcct tttcttctgc ttttccgttt ttgacccaca aatacagctg aactaaagcc
    13861 aaaagagcag ataaaatcat tgaaagaata gctaagtagc gataatggac attgatatat
    13921 tgatcgagtt tgccagaaac ttgcaagtac atcatcaatt cgaaataccc agataaaatc
    13981 aaaaaccgca gcatattttc cctcctaaat aaataatgtg aatccaataa cgctaagggc
    14041 aatcagtccg atcagcgata aaataaagcg tcctgtaaaa tatcgtttca tcatcagtag
    14101 atttttgata tcgaccattg ggccaaagac caaaaaggca gctacaggtg ctgtgccgaa
    14161 cagacttaac aaagaagatc ctataaacgc atctgcttct gagcaaagag acatcgtaaa
    14221 ggccaataac agcatgatga taatggctaa aatctttgtt gagccgagcg ttagcataat
    14281 tctagttggt aaataagtct gcatcgcagc tgcgattaat ccgccaatga tcaaataacg
    14341 acctgtatca aaaaattcgt cgattgaatg tgtcaacaca tgcccagttt gatggatcag
    14401 ctgatttttt ggttcattct tctcactttg ttcttcttca tggacatgat gatggtgatt
    14461 ttctgctact ttagacgtga ttttttcttt caatacagcg tctttttgaa aataggcaag
    14521 ccagacacca atcacaaggg caacaaaaaa gctgcctatc acccgccagc ttgccatctg
    14581 ccaagaattg ccaaatgcga taaaagtgga aaaaatcacg attggattga tgatcggagc
    14641 agtcagcata aaagcaaacg ctgtgtgaac tggtacgcct tttttaacaa attgatgaac
    14701 gatcggtacg atgccgcatt cacatgaagg aaagaaaaat cctaagatcg aaccagtaaa
    14761 aatagagaag atacgatttt caggaagcca tctcttcaca cgttctgggg tcaaaaatac
    14821 ttgtaaggcg cctgagataa gacagcctaa taatacgaaa ggtaatgctt caataacgat
    14881 cgataagaaa atcgtactca tctgcaaaac cgaatttgga ataaaagaaa acatgagttt
    14941 cctccttttt atttctagtt cattcgcttt gcaaatatac gcttttattc aaaaaaaaca
    15001 agttgctctg taaattttta cacaactctt attttttacc aaagcacttc aattgacttt
    15061 ttctgttatt cccattaaat taaggtctca tacatatcac ttctatcata aaagggagaa
    15121 taaaaatgaa acaaaccact aacttgactg aagttcaaga catactgcaa cagtcatccg
    15181 ttgccgtcat ttatctttcg atgcctaatt gcagtgtttg tcacgctgtt cgtccacgac
    15241 tagaggaact tctcacacat tatgatattc cggcacttca tttggatgca tttgaaacac
    15301 cagaagtagc tagtaaattt gaagtgctga ctgcgccagt tgtcctgatt tttcatcaag
    15361 gaaaagaagt ctttcggcaa gcacggttca tcgactttaa aaaaatccgt tatctactag
    15421 atgaactgac agcagaagat gattcccttt cttatgaaga aatcttccga acaaaataat
    15481 cgttgtccaa acagaaaaaa gcgattgaac aagtgaaagt tttgccttca tttgttcaat
    15541 cgctttttat ttttattctg ctgtattttc ggaatgcgca tttgcgtcag aatgcaagaa
    15601 atagatcaag gtttgcaatt cattcgtcaa atcaacattt tggatcaata catctgacgg
    15661 agcattcaat cgagcagcgg taaagttcaa gattcctttg attcctgctt ccgttaactg
    15721 attgactact tcttgtgctc tttcagcggg tatcgttaaa attgctactt cgatttgttg
    15781 gatcttgatt tgttctgaca tatcttccat tggataaacg gggataccgt ctacgatgcg
    15841 cccaacgatt tcctcattca catcaaaagc acaactgata cgtatgctat tactttgatg
    15901 gaatttgtat tttagtaaag cacttcccaa gttccctaca ccgatcagcg caacatttgt
    15961 caattgatcg tcgtttaatg tctttgcaaa aaagtgcata agatcttcta catcataacc
    16021 atacccacgt tttcctaatt cgccaaaata agaaaaatct cgacggatcg ttgcactatc
    16081 tacttgaacg gcttcactta attcagttga agacactttt ttctttccag cattatttaa
    16141 aatccttaaa tagcgataat acaaaggcag tcttttagct gtcgcctttg gaataatatg
    16201 atctttcacg aaatgacctc cacgtattgt ctaattacat ctttgatttc tccatcatag
    16261 cagtatttat aggaaaaaat caattccatt gctcagatat tgtgaaattt tattctttat
    16321 tcatttattt atcatttctg caaatctcgc agtataatgg aatttatggt agactactaa
    16381 ttgaaaaata gttttttatt tgaggagaat tcacaaagta gaaaagagga ttccatgata
    16441 ttattacaag ccaatcagat tgctcgccat tttggtgcag aagtcttatt tgaaaacata
    16501 catttagaaa tcagtacagg cgctcgtgtc gctttagttg gcagaaatgg cgctggaaaa
    16561 tcgactttat taaagatcat tgctggaatt gaagcacctg atgaaggaaa gatcgctaaa
    16621 aataaaacag cgactctcgg atacctcgct caggacacag gacttgattc caatgaaaca
    16681 gtttggaacg agatgctgaa agcttttgaa caagttagac aaatggaaga gcggatgcgc
    16741 gagctagaga ttttcattag cgaaggtacg ccagacacac ctgcttatag ttcttattta
    16801 aaagaatacg accggctgca acacgatttt tcagatcgaa acggctatgg ttatgaaaat
    16861 gaaattcgtt ccgtattgca tgggtttaaa ttcgattctt cattttatga tcagaaaatc
    16921 gatactttat caggcggaca gcgcacacgt ttagctttag ctcgcatgct tttgcaacac
    16981 cctgacattt tgattctaga cgaaccaacg aaccatttag atatcgagac actttcctgg
    17041 cttgagaatt atctgcaaag ctatactggt gcattattga ttgtttccca tgaccgttat
    17101 tttttagata aagtagtcaa tgaagtctat gaattaagca gaagaaaaat gcgccattac
    17161 aaaggtaact attcgaaata tctagatttg aaagcagaac agttagcaag tgaatggaag
    17221 gcttatgaaa agcagcaaac agaaatccat aaattagaag acttcgttgc tcggaacctt
    17281 gttcgcgcct ctaccacaaa acgagcgcaa agccgcagaa agacattaga aaaaatggac
    17341 cggctggatc gtcctcaagg ggatgaaaaa caagcaaact ttctattcag tatagaaaag
    17401 ccttctggaa atgtagtctt gcaaatcgaa gatgccgcta tcggatatga taatgtcacc
    17461 ttatctgaac caatcaatct agatatccgt cgtcaagaag cgattgctct agtaggtccg
    17521 aatggtatcg ggaaatcgac gttgctgaaa tcaatcattc accagatccc ttttatccgt
    17581 ggaaaagaaa cgctagggac aaatgtcacc atcggctatt acgatcaagg tcaagctgat
    17641 ctgcatacaa acaagacgat cctagcagaa ttgtgggatg agcatcctac cactccagaa
    17701 aaagagatcc gaagtgcttt aggtgctttc ttattttctg gcgaagatgt agaaaaacca
    17761 atccctctgc taagtggcgg tgaaaaagca cgggttgctt tagccaagtt agcgatgaac
    17821 aaagaaaatt ttttgatttt ggatgaacca acaaaccatt tagatattga caacaaagaa
    17881 gtattagaaa atgcattgat cgattatgac ggcacactgc tttttgtttc ccatgaccga
    17941 tatttcatca atcggatcgc gacaaaagtc gtcgaactct ctgaaaatgg cagcaaagtc
    18001 tatctaggag actatgatta ttatttagag aaaaagcagg aagaagaaga aatcgccgaa
    18061 ttgttggcac aagatgagcc agaatctgct cctgctccca cgaacaaatc tgattattac
    18121 caaagcaaag aacagcaaaa gcttatccgt agccttcaac gaaaaattac tcaggttgaa
    18181 gaagaaatgg cgcggatcga tgaatggatc gatcagttgg aacaagaaat ggtcgcgcca
    18241 gaaaacctgg aagatcacgt aaaactcaat gagctgaacc aagatttaga agctgctaga
    18301 caagatcaag aaacaaaatt gacggaatgg gaagaactaa gcttggaatt agaagaatat
    18361 gaaaattaac gaaaaaacca aaacagaaac cattcatgct gttttggttt ttcttttttt
    18421 attttatcaa acaaaaattc catatttacc tttttcacca ataagaagac caattactag
    18481 tcattctcac taaagcttct aaataatagt aatcacccca aatacaaggt gtatttaccc
    18541 cattattttt gggcttatta taaacagact gtgttaaaaa gccatcttct tcttccgttg
    18601 tagcatatct ttctttcaat tgtcgcataa ttttccaaac agcagactgg tataagttct
    18661 tttctgtttt gtctaataga cctgattcta ttaattctaa taatccacaa accatgatca
    18721 ccatactaga tgtatctttt tcttctctgc tagaatcatc aaagatcaaa tcccaataac
    18781 aaatggtatc ttctggcaac cgattcataa aataattggc taatattttg cttaaatcaa
    18841 aatatttttg ttctttcaaa taacgataac ttaatgcaaa accgtaaatc ccccatgctt
    18901 ggcctctagc ccaacaggaa ttatcactat aaccttgatg cgtacttcca tatcttggtt
    18961 ttccatcttt gacatccata taaaatgtat gataagttga accatcttct cttactaaat
    19021 aatctgctgc attttgaata tgagcctgag cagctttttt atatttttga tttccagttt
    19081 cttcactcgc ccaatacaat aatggaagat tcatgcaaca atccatgatc attctacctt
    19141 gttgtgaagg atcactagga tctccccaag cttgtataac tcctgccttc tcccaaaaac
    19201 gctgaagtaa tgtttctgca gcatcaattg aagcttcctt catttttcga tttcccgtta
    19261 ttttataatc tgctacggtt gataaagaat acaaaaaacc aagatcgtga tgattagtga
    19321 taatttgaca gtcaatcctg tggataaaat ctatatctgc tttttcagct gtttgttgaa
    19381 ataaaggatc acccgtcttc tcaaataaca accaattaat tcctgtccaa aatccttctg
    19441 tccaaccaat attgtctctt aatggataac gtccttttat aggacactcg cttgggaatt
    19501 gatactgact gttactgata cgtttgatta acctctccgt gtgtctgcga caacgctcga
    19561 tctcattatt ccattctttt tccattaatt ctttataaca ttcatttatc catttttctg
    19621 gatgtattat ctcttcaaac atcactttat caagcatttc cccaccagcc tttagcataa
    19681 ttttagtctt tttttattgt ttttattttg gatagttagc ccggttgtcc catcaaaatc
    19741 aattttggga cgattgttcc atacttctaa actataatcc cgatcttgat gagcaaccac
    19801 tcctgttccg agccaatggg ttccttttcc aaatgtaccc tctaatccag gaataaaagt
    19861 gttttcccat gtcatcaagt ttgtattggg caaacccata atagctgtgg attttcgttt
    19921 aatagtatcg tcacttaaac agataatacc tgaaaagcca gcttcactca ccctatagtt
    19981 ttccttgtct tcttctatga ttattccttc tttctcagga gaacattcaa tagcaaaccc
    20041 tgtctcatat agaacatatt ctgtatctaa ttctactttg tgtacgcgaa tctgccagcc
    20101 ttcaaaagga ataagccaag ttctgatttt tgcctgtaaa gtaggttccc atgtagaggc
    20161 aagtacctct ttcttacata caatgtctcg tacattattt cgaacgatga actgtccata
    20221 ggttttgatg ctctcattct taaaactggt cgtgatatat gtgtccgctc gctgaatccc
    20281 tagcattgaa tccattgcca tttctgcttt caattgattc gctcgcggta tcgaaaaacc
    20341 aaattgacta gaataagcaa attttaaata tttttcattg gtcaatgtat ggaaatttgg
    20401 ccctaattgt cccgcattca aaaaaaatgt atgtccatta tcatgttgta tgagcaaatt
    20461 ggcttctgaa agaagtttag ttccttcacg aataggcata ggcatttctt tagccgtcca
    20521 atactcatgg tcttcaggta gcgccaacaa taaaaaaatc ttattcagcc ataatggaga
    20581 taacgtacta ttatagggtt cagtcaatgt caattgaggg taggtataac caatattcag
    20641 gatacctttt tcatcaaata tttctttttg catccaccat cttatattcc gattgactaa
    20701 ccccttgatt tcacctaatt taaaaggcaa aagatcggcc cacaacatag ctgaccaaaa
    20761 gcttgaaact gcaaaacgat aaatcatgct tcgaccaaaa ggcacatttg caccatcttc
    20821 cgcaaaaaaa tgaatatagt ctctagaaaa atcaatagct cggttcttat atatctggct
    20881 aatgggcttt tctggttcta gtacgctata aatcaaacca taataatgaa atgcaaaagg
    20941 attataataa tcttggcgac ctcttgaact gtctcgatac cagccatttt gaagataaca
    21001 attttctata ttatcaaatg cttctttcag tgctgattct ttaactggat aaccaaattt
    21061 ttggataatt gttccgacaa ttatcttaaa aaattgccaa ttaccatctg cacattcata
    21121 ttcaaaaatt tgcgagaacc aatgaataat ctggcttttt tctcctgtat ctaatttttt
    21181 ccataataat tgttcatgat gatataacgc taataacatt gcgggcattt ctaccatacg
    21241 ctggtctctt tcatgtattt ctccccaata atcgggatgg gaaggatctg ttccagcttt
    21301 tattcctgat agaattaatt tcaaccatcg ttcatctaat tggtcaattg cgggccccgc
    21361 tccccataaa acccgagaga atccttccat tcctgcaatt ttatttccat atcctacccc
    21421 ttgatgtata taatcaattt gtgcgccact tgctcgataa aacggctgga gtggttgatt
    21481 tatcttttta aataagtcaa agtaatcctg tctggtcctt aatagtggta ctggatgttt
    21541 cattgtattt tctcctcttt ttactaccaa atagctaatt ccgactgttc taatcgaagt
    21601 aagccttcaa taaaaaaata atcagaatag atcagtggca cattataagt cagttctcta
    21661 tggtatgctt ctgctccgcc tccaatgatc ccatcttcat ccatattcca atttgcatag
    21721 tgcttttctg tcttttggag tatattgata gcagcttcat gatacatttt tgatcgtaca
    21781 gaggaaacat atcctgctaa ttccagaaga ccgcatgcag cgcatactcc agcactggta
    21841 tcaatattcg aatcttttac agcaaaatca atgagaggaa tataaccagt tttttcgatt
    21901 ttctgaataa aatttactgc tatcttttct gctatagcta gatagtacgg attttttgtg
    21961 taacgataac ttaacgcgaa tccatataaa gcccatgcat ttcctctgct ccaacaagaa
    22021 ttctcattgt atccttgccc gccaagtaac cgaatatatt tccctgattc tgcatcaaat
    22081 tctgcgatat gcccggttga tccatctttc ctcactagat acttagctgt tgtatctgca
    22141 tgctgccgag caattttaga gaacctcgga tcaccagttt ccctcgaagc ccaatacagt
    22201 aatggtaaat tcatcatact atcaataatc atccaaccag gtttatggtc attccatgct
    22261 actaaatagt tccctgaaat attatagcgt ccagcaagta ccgtagctgc atgtaaccct
    22321 agttgtcgac tatgtttgtt ttgagactgt tgataatgtg caatagccga tggtaagaat
    22381 aaaaaaccta catcatgatg aacgtctgaa aaatggtaca gggcagattc taataattgt
    22441 tcttgttgta ttgctgcctc tttgaacttt tctcgttttg tcttgtgaaa gagctgccat
    22501 aaaataccac tccaaaaacc attggtccac cagtcgattc ctctgtcctt tattacgtct
    22561 gtatattttc cgttgtaaga aatatatgga attttattcc ccattctgtc taattccgca
    22621 tctatctttt cagaagctac attcaaaatt tttcttgacc attccttcgt ctgttcattc
    22681 atggatatcc cgccattctt ttaattttta tgaatgtgga gttgctccta gaacgaacta
    22741 gatggttaac attcgttatt ttttttactt atttgcagct ccaatgtcat tctccttaaa
    22801 tttagactgc gcccacacat aaatcaagac cattgggatc actgaagttg ctaaaattag
    22861 cataattgcg ccataattaa ttggctcacc tgtactagaa accgtcatat ttttgataat
    22921 taagggaacc gtatatttat ctttatctga aagcatgact aatggaaata gatattgatt
    22981 ccaacttccc ataaagataa gtaaaccact agtaataatc gtaggtgata aaatcggtac
    23041 agcgattttc gtaaaaatca cccactcatt ggcaccatcg attctggcag cctccatcag
    23101 tgctgtagga aattgactgc tatattgtcg catcataaac actgaagttg tactggcaat
    23161 cgaagggatg atcacacctg cataagtatt cgataatcct aaacgactca taatgtaaaa
    23221 taaaggaatc aatgtggtaa aaaaaggcac catcctagat agcataactg tattgaacaa
    23281 cagtttattc cccataaaac gatactttgc taatgcataa cccgccattg tagaactggc
    23341 tgtagctgaa actgtactga ttacagcaat aacaatcgaa ttccaaataa tcaagggata
    23401 attatatctc gccatgatgg cttgccagtt ttttaatagc atatccccaa acttgaaatt
    23461 gattccgcca cttaaaattt cttgattact ttgcgttgaa gctacaatca tatataaata
    23521 tggcatcaaa aatattaagg caccaaacac aataatgacg tacgtaaaaa ttctgctttg
    23581 tatcttttgt ttagtcattt ttatcacctc cgaatttaaa ttgaatagcc gataatataa
    23641 cagatacaaa caccatcgtc caggcaatag cagaggcttt tccaaaatca accatttcaa
    23701 aacttgtttt atataaattg actcctacga ccaaagctga attttctggt ccattggaag
    23761 tcccaaagaa tatatttggg atattgaaat tacccaagcc attgatagtg gctaaaactg
    23821 tggccattaa aatgattggc tttagcatgg ggatcgttaa taaaaagaat tgccgaaatt
    23881 tcgatgctcc atctatttca gcactttcgt atatctcttt agaaatccct tgaagaccag
    23941 ctaaaaataa aatagtgtta tatcctacac taagccaaat catcgtgatg atcattgata
    24001 ttcgtatcca aaaaggattt gttaaccact gtacaccgtt aattcctagc gcattcaaaa
    24061 atccattgaa taacccagtc ggattgaaaa aagtcatgaa tacggaagct acagccaccg
    24121 ttgatgtaat ggttggaata taatagacag ttctaaaaaa tccttttcct tttaactgtg
    24181 ctgaattcaa taaaacagca aagtaaatag ctaatagcat cgttattgga attgatcctg
    24241 ccatgataat caaaacattc gtgtaagatt taataaacaa tggatctcgg aagatagcta
    24301 gataattgtc aaatccaaca aattcaaatg agtagccaat tttcataaaa ctaaaagcaa
    24361 acgagataat gatgggaatc aaaatcgtca caataaagaa aataacaaaa ggtgtgacca
    24421 atccataagc acttttttta tgagacgtat ctgttgcttt tttcttgctg ttctttattg
    24481 tcttgcttgg taacgaagat tgcatcatcg ttaagtcctc ctaatatttt ttaatcacca
    24541 gttaatttta cgccgtactt atgagcaaaa ttttctgctt cttttagata tgaattttca
    24601 aaatcacctg tttgccaata gttatatgca gcggtagtca aataagacgt agcatctgaa
    24661 taagtggctg gtaaaacagt atttgatgta ttttcattga ttttattcaa tagttcatag
    24721 tatttttgtc ctccttagta gtcaaacttt ttctcagctg cttctgtttg ataggccaat
    24781 gagttagatg tagccacgcc cagctccagt gcgttggcta agcattcttc atctgtcagc
    24841 ataaatgtaa gaaattgttg tgccacttgt ggatttttgg catctttagg gacataaaaa
    24901 cttgatcctc ctgatactgc accttttcct ggattttctt ttgaaaaagg aatcagtgga
    24961 gccatgcgcc atttgtcaga tgcatcagga aaattcaaag acatattcgt agcataccag
    25021 ccgccagcaa caaacattgc agattcttga aatgctttct cagcatcttg tgcaccgtag
    25081 aaatgtacaa tatctgagtc gatcatttct ttgattaact ttgcagcatg tttcgcttct
    25141 ttagtattca gacggatatt tccttcttca tcaagcaaag attgattctg ctgggctagc
    25201 atgttgataa acaattcttg gtccccagat tctggcaagg caattccaaa actaccagtt
    25261 ttttctttta atgcttttgc cacctggata tattcttccc aagaactgat ttcttcaaaa
    25321 tctacaccta tttcagcaaa tttttttggt tgataataac aaagtgctgg ggtaaaatca
    25381 ctagcccaag gaataatcgt ttcattactc gtttgttgac gcaaaatatt caatttttga
    25441 tcataaaact gatcgccata tttgtcataa aatccatatt catcagcact ataaaaagca
    25501 tcttgaaatt tttccagcac acctgctgct gaagtgtctg ccatggagat aatatccggc
    25561 atatcttcgt gtgttaccaa ttttggggtg atcatttgta ctgttgctgt tcccgaagct
    25621 acattcacaa tttttaagtc aatatttgct ccagttttgc ggttatagat ttttgcagca
    25681 ttttcaacat agtttgtcag tttgccgttt gcgacatata cttctaatgt agtggctttt
    25741 tttatcattg gatctgcgat gtttgctgtt acttcttcgt tagtttttcc acatgcactt
    25801 aagctgaata atagggtaat gtttagcaaa gctatcacca atttgtatct ttttttcatg
    25861 tctatccctt ctttcttttc aagttcttat ataaaacgct ttcacttatg tatattaacg
    25921 gaattagtaa cacaaatatt tatataaaaa aatattttac ttgtcgtttt ggcttttcat
    25981 ttttgttata atagtcatta attataaata gaaagctttt tatataaaaa gagaaaggaa
    26041 agtggttctt ttgttaacaa acaaccaatc ctccaatctt gtcaaaaaaa cactaaccta
    26101 ttccatagat acccatcttc atttatctgc tgctccctat tttcgactac taataaatga
    26161 aagcgctaat ttatatgctg aatttgcaca taaatctata gaactagata atccttccct
    26221 tttattcatt gataaacagc aagaagtcca aatcttttct actcaaaagg aaacacaaac
    26281 ttttatcatt atccagtttt attttgatga gcaattatta gcacatatta cgggacaatc
    26341 tttttctata gatcaagaaa taacatctat cattactttt ctgaaaaatg aacagttcaa
    26401 aattgattat ctaaaacata gtaagccagc tatttcctat gatcaggaaa tcaattcttt
    26461 tttcatacta tgccaacaga tgagcattca tttgctttcc ttattagtta cttattgtct
    26521 gcaaaaaatt caattgtctt ccttaccaag gcattatcaa caggaacttg ctttattgac
    26581 aataccttca aatgagaaag tcattcctaa aaaaaagcat attctttatt cagatgaatt
    26641 agttgatcca atcatacgat atttagaaga acatctttca gaaaattttt tgcttgaaca
    26701 attagcccaa atttttttta tcagtccagc cagcttaaaa aaacatttta gaaaagtgaa
    26761 t
//
LOCUS       DAHHZJ010000023        26625 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.024, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000023 DAHHZJ010000000
VERSION     DAHHZJ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.024"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(19..729)
                     /locus_tag="NBL02_000785"
     CDS             complement(19..729)
                     /locus_tag="NBL02_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HCD2571491.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(726..2198)
                     /locus_tag="NBL02_000786"
     CDS             complement(726..2198)
                     /locus_tag="NBL02_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HCD2571492.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            2336..2809
                     /locus_tag="NBL02_000787"
     CDS             2336..2809
                     /locus_tag="NBL02_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCD2571493.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(2962..3513)
                     /locus_tag="NBL02_000788"
     CDS             complement(2962..3513)
                     /locus_tag="NBL02_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCD2571494.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            3618..4148
                     /locus_tag="NBL02_000789"
     CDS             3618..4148
                     /locus_tag="NBL02_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HCD2571495.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(4631..5074)
                     /locus_tag="NBL02_000790"
     CDS             complement(4631..5074)
                     /locus_tag="NBL02_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD2571496.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(5090..6250)
                     /locus_tag="NBL02_000791"
     CDS             complement(5090..6250)
                     /locus_tag="NBL02_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCD2571497.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            6757..8457
                     /locus_tag="NBL02_000792"
     CDS             6757..8457
                     /locus_tag="NBL02_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571498.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(8559..9392)
                     /locus_tag="NBL02_000793"
     CDS             complement(8559..9392)
                     /locus_tag="NBL02_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCD2571499.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(9696..11861)
                     /locus_tag="NBL02_000794"
     CDS             complement(9696..11861)
                     /locus_tag="NBL02_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCD2571500.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            12121..14415
                     /locus_tag="NBL02_000795"
     CDS             12121..14415
                     /locus_tag="NBL02_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HCD2571501.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            14408..15073
                     /gene="pgmB"
                     /locus_tag="NBL02_000796"
     CDS             14408..15073
                     /gene="pgmB"
                     /locus_tag="NBL02_000796"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCD2571502.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            15066..16073
                     /locus_tag="NBL02_000797"
     CDS             15066..16073
                     /locus_tag="NBL02_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCD2571503.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            16095..17114
                     /locus_tag="NBL02_000798"
     CDS             16095..17114
                     /locus_tag="NBL02_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCD2571504.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(17395..18762)
                     /gene="nhaC"
                     /locus_tag="NBL02_000799"
     CDS             complement(17395..18762)
                     /gene="nhaC"
                     /locus_tag="NBL02_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HCD2571505.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(18871..20292)
                     /locus_tag="NBL02_000800"
     CDS             complement(18871..20292)
                     /locus_tag="NBL02_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD2571506.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(20535..22412)
                     /gene="tdc"
                     /locus_tag="NBL02_000801"
     CDS             complement(20535..22412)
                     /gene="tdc"
                     /locus_tag="NBL02_000801"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HCD2571507.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(22702..23958)
                     /locus_tag="NBL02_000802"
     CDS             complement(22702..23958)
                     /locus_tag="NBL02_000802"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCD2571508.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVGALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(24070..24275)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            24538..25839
                     /locus_tag="NBL02_000803"
     CDS             24538..25839
                     /locus_tag="NBL02_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCD2571509.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(25908..26507)
                     /locus_tag="NBL02_000804"
     CDS             complement(25908..26507)
                     /locus_tag="NBL02_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCD2571510.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT         8079 a         5482 c         4769 g         8295 t
ORIGIN      
        1 tacatggtgt tcaaaatatt attcgccttt tgcttctttc actaattttg cgaatgcttg
       61 ttgttggaat tcagcagcta aggctttcgt acgttgttct tgctctgctt tgctcatttt
      121 tggattttca gtcattgctt ttaccatacc gtcttgaaca taagcttttg cttcactttc
      181 catcgtactt tgctgtttct tcactaattc agctaacttg gctgcttggt ccgtacttaa
      241 ttctacccat gtctctggga ttttgaatgt attcactcgg ctgtcatatt cgtgattgtt
      301 atcagcaata ttgaaccaga atttatacat gtcgtttttg tattcccaat aagtagtttt
      361 cgtcactttt tcagcatcgc cgtctgtctt ttctacttta tttgtttcgt tatccgtacc
      421 agttgctttt ggtttttctt gtttttcatt tgttcggtaa agatacactt tttcttttcc
      481 attccctaaa gctttataca aaagcatatc tgctccttgg gaatcagcac tggaaacaat
      541 tttttctgtt tttgtttccg ttactttttc cattccaaag tgattggaca aattagcgac
      601 gatcaaactt agtgaagcga cgaagattag tccgaaaatc aaagataaaa aggtttgcca
      661 tgttttttta gcgaacacgt tcgttaaagc aaaagcaagc aagctgacaa tcaaaactcc
      721 aataatcatg cattatcccc cgctttcatt agaacaggtt cttttttctt caataaaaag
      781 gcaataataa agccaagaat cccaaagatg atcgctacga taaacgcggc atggtaacct
      841 gacagtgtgg cattgattgc ctgatcttta taagctaatg gcgtattatc caatacagat
      901 ttgcttggga tatttgcttt tgtcacattc gtcaacacac tgatcaaaat agctgtacca
      961 atcgagcttg cgatttgacg gaatgtatta tttaccgctg tcccatgact gatcaagtta
     1021 tttggcaatg cattcatacc agaagtcgtt accggcatca tgaccatgga gatcccaaac
     1081 atacggatcg cataaaggat cacgatatat aaaactggag tcgtttttgt caagaaagca
     1141 aatggtgcag ttgccgctgt caaaatgaac atccctgtga tggctaaacg tttcgcgccg
     1201 tatttatcga aaatcgcacc cgtcacaggc atcatagccc ccatgatcaa tgcacccggc
     1261 aacaacatca agccagaatg gaatgctgat tctccacgaa tattttgaat atacaatggt
     1321 acgaccattt ctgcccctac catcgccata tttgttacac cagctaaaac agctgcaatc
     1381 gtgaatgtag atgatttgaa tacacgaagt tccaagaatg gatgttccaa atgtaattga
     1441 cgccatgaga acaaggcgat gaaaatcaca ccaacaacta agaaaccaat tactttcaag
     1501 cttccccagc cgtcatctcc tacactagaa aatccgtaaa gtaaactacc gaatccaatc
     1561 gttgataaaa tagcggataa aatatcaatg ctaggatttg acaatggaat aacacttttc
     1621 attaggaaga atgctaaaac caagacaaga acaacgattg gcagtaccat accaaacaag
     1681 tcacgccaat ggtagttatc aatgatataa ccagataatg tagggcctaa agcaggagca
     1741 agtccaataa cgatccctga catccccatt gctgatcctc ttttttctgg cgggaaaata
     1801 gatagcatga tattttgcaa taatggcatc gatactccca caccgcaagc ttggatcaaa
     1861 cgaccgatta gtaatgtact aaaattaggt gctgtaaagc aagtaatcgt tccgatcaaa
     1921 aagatcgtca tggcagaaag atataaattt cttgaactga atttattgat cagccatgct
     1981 gttattggga tcatgatccc gttgactagc aagaaaccag tcgttaacca ttgcacatca
     2041 gacgctgaaa tatcgaaagc tttcatcaat gtaggaaaag ctgtcgtcaa taaggtctga
     2101 ttcaaaacgg tacaaaatgt accaattaat aagacaacaa ctaaaagtgt ccgattatat
     2161 ggttttccgt aaatatctac gggttggttg ttactcatat ttctccttct ttctgaattt
     2221 tttctgaaac tactagaata ctttagtcct aaattcgcta tttgtcaacc tacttgacac
     2281 ataagttaat acggatataa ttaagggaag caagaggaca aaaggagtga caaaaatggt
     2341 tggggcttcc cttaaacgtt tgttggaaaa tgatcaattg gttgttggca tcagtgaatt
     2401 aagtgatatc gtaggtgtgt ctccacgtca attaaggtat tgggaacaaa agggattcat
     2461 tcgttcgatc gaagctgatg cgaattgtcc tagaaaatat cgtttaccta cagtcataaa
     2521 agtagaactg atcaaaaaat atttagatga cggatttact ttgtcaaaag ctgcagaaaa
     2581 ggcagaaatc cgccaaaaga aaatgcacca tgtccgtaaa gttttttcaa aagcaatcaa
     2641 agatatcgaa ttgatcgacg aacgctatac gattttacaa atcggtaact tcaaagaaga
     2701 gaatgctaag atgtacatca ttcatgacaa cgagacagac gaattaactt acaagctcct
     2761 tcctgtcgat gaaaaagctg actacgaaca gttgttaaaa gaactctaaa cgccactaat
     2821 ctcctaagta ccaagctttt taataagtag atgctacata aatacttgaa aaaaggcctg
     2881 tttgaacata agaatatccg aacggtgatc tgcgtttctt tcatatagaa aggaattctc
     2941 catcactgtt cggatatttt tttatttttt tcggttactt tcttctaata catctgttag
     3001 ccaatttgca tattctgttt cccgattgat cgcatgcgct aaaatcaggt agtgtccgta
     3061 attttcatgt ttttcttttt cagtaggaaa gatcgttttt tttcgctcta atagatgaag
     3121 tcgtttttct tcatgaaggt cacgctgctg ctgaataatt tctactaatg ccggatcatt
     3181 cacgtctttg acgaaataca atttcagaac aaattcgtct cgattaaccg gtagttcatt
     3241 ggtgggtgta tgaatccact ctgccaattg ttccttccct tcttctgtta attcataaac
     3301 tttcttttcc aatttttcac ctgtgatctc tacttgatgc tgaatgattc cttgctcttc
     3361 catttttgca agttcaggat aaatctgact atgttttgct tgccaaaatt ctcctatttc
     3421 atttttgaac gattgattca attcatatcc tgtcatcttc ttcttactta ataaccctaa
     3481 caaaatatat cgaagtgtat tttttcttgc catcagtttt ccctccttga caaatcttta
     3541 aatccattct atcataaatg agtaatcgaa aacatgtagt ttattacatg ttttaattta
     3601 taaggagatg cttaaacatg aaagaattta aagaattgga agattttatt ggaactcact
     3661 ttatctatac gtatgacaat ggttgggaat atgaatggta tgccaaaaat gaccatactg
     3721 ttgattaccg tatccatggc ggaatggtca aaggccgttg ggtaaaagac caagaagctg
     3781 atatcgttaa attgacggac ggcgtattca aaatcacttg gacagagcca acagggacag
     3841 acgtggcatt ggactttatg ccaaatgaaa agaaacttca cggaatgatc tttttcccta
     3901 aatgggttga agaacatcca gaaatcactg ttactttcca aaacgaacat atcgcagaaa
     3961 tggaagcagc tcgtgaaaaa tatgaaactt atccaaaatt acttgttcct gaatttgcta
     4021 cgatcactta tatgggtgat gcaggtatag acaatgaaga tgttattagc gaagaaccat
     4081 acgatggaat gccagatgat atccgtagcg gaaaatactt cgatgaaaat tataaacgag
     4141 taaaataagg ttgtgaaaga gaggttgtga aatcagcgtt ctgacctgag tattagagaa
     4201 agaaacggaa aaatagttcc tcatgttttg ccgttttttg agctaatacc gagggtcagc
     4261 tgattgaaca ccgtttatca agaggttgtg aaatcagcgt tatgctttga gtaataagaa
     4321 cgctagttag aaaatagctt ttcatatttt ctaggctagt gggcttatta ccgagaagca
     4381 gccgtttgaa caccgtttat caagaggttg tgaaatcagc gttttgacct gagtattaga
     4441 gaaacaaatg gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc
     4501 agctgattga acaccgttta tcaaagattg taacaactgc atattcacac agttaaatta
     4561 gtgacttcaa aatgaaataa tgctcccagc aaaaaattag gctgggagca ttatttcgtt
     4621 attctgttca tcaaaaatca gcgttttgat tccatttcat cacaagttcc tcctcattta
     4681 ctgcttcatc tttttgctgt gtttgaattg taaaaccttg tgaaagatag aaatgaatag
     4741 ctgattgatt tttttgatag actgtcaaag tcagtgtttt attggtttct tttatttttt
     4801 tcattagttg cgtaccgatt ctttgttgcc ggtattccct tttcacaaag attcctgcga
     4861 taaactgatc aatcacccca gcaaaaccaa taatttcttg atctatttct gctaagtaaa
     4921 tcactgcatc tggtaaagct ttcctaacag attctacttg attctcccaa taagttgatg
     4981 gaatgaattc atgagaatcc aagttagcat gcagccaaat cgatagaaca gattccagtt
     5041 ctttggcaga caattttgtt gttgtgcgga tcattttttc tctccttact tattcattga
     5101 ttttaaaata aaaaccattt ttttgaagct cttttacaag ttttcgcccc tcaaaaaagt
     5161 atatcttctc ttgttttact tttctaatac caaaagaaac agcaacttta ggatcatcag
     5221 ctgaaacgat tcgataagtt ccaccaggca attgatccag cttgactttc ccatgatggt
     5281 cgctttgtac tgaaaacggc acaccatttc tagtaagtgt ttttttctta tgtttcaaaa
     5341 tgaatgaact accatttacc ggtttccctg acgaatcact aataataaat gaaaaatcaa
     5401 gcactttttt acttaatcta tactgataga ttagtcgtcc gataaacact aacagtatga
     5461 ctacagctat tatcgttcct gcaagaataa accattgccg catggttctt tcctgatttc
     5521 cttttccttc ttcttttttt accgattcgt tataaggaat ccgatgtcct gtcactagca
     5581 aacgatgcgt gttgatcata tacggcgtac atgtaagcaa ggtaactaag tcttctcctt
     5641 ctgaaatcgt tagaacagac gtgtcatctg gcagtaccac ttgtatattt ttaacttgat
     5701 aagccaattt ctcccccaaa acagtcaaaa taaacgtgtc tccgttttct aaacgatcta
     5761 attcggtaaa taaccgtcga ttaggtaaac cgctgtgagc agagatcact gaatgcgtcg
     5821 aatttccacc aagcgggaaa gaggttcctt gcaagacgcc tgctccattt tcaaggatcc
     5881 taccattgag ccgatcataa agtgaaatat ttaattggat cttaggtata ttgatacttc
     5941 cgatcagcca ttcctctaat tggctggatg ttgtactttc atttctgttc ttttcattga
     6001 aaggatcagt accaggattg aatccttggt cagttaatcg ttggtttttc tcctccattt
     6061 tctttcgctg cacttcaatt tcagctgcgg tccgtttttg tacttgttcg atacgttttt
     6121 gatcaataaa actatttaaa gaatccacat aaaatggata taaagcgatc agtaacccta
     6181 cgacgataca tattaataaa aacaaacgaa acaaaagtcg acgagactgt ttttgcttac
     6241 tctgcttcac atgtgtcctc ccttgcaagt ttttttgttc tttcttttat tatatgggtt
     6301 caggaaaaaa atagaaaatg aaagccgatg aggttgtgaa aaagctgtcc tgcatcaagt
     6361 aataagtaca gcccaaaaaa atagctactc atattttttg ttgggttggg cttattaccg
     6421 cagatgcaag cttttgaaca ccgtttaagg aagaggttgt gaaaactgcg ttatgctttg
     6481 agtaataaga gcgctagtca gaaaatagct tctcatattt tctaggctag tgagcttatt
     6541 accgagaagc agctgattga acaccgttta ccgagaggtc atgaaaaagc tactttgttt
     6601 cgagtattaa gaaaaattct gtttcaaaac aaggagatgc aacaaaactt atgtcgcatc
     6661 ctctctcctt agaactttca cttatgtttt tcttgtaaaa ttcataaata ttttcatgaa
     6721 ttttctaatt tcttgcctac ctctcgcata ggatatatga tgaatttatc taaagtttgc
     6781 aaaggagttc gacaattgaa aaaatatggc ccatttttag caattttttt cattgctttg
     6841 gcatttctca tgcctcattt attcacacat aaactaattt tgggtgctga tgccccattc
     6901 cattacaatc ggttctatga tgccgcgcaa caaattaaat atggggattt ccattatttt
     6961 ccttcccttt atggattcca gcaaagtggc aggatcgtga atgccgttta cggcccattt
     7021 ttcgcttatt tacaaggatt gatcgtgttg cttgcaggta gctggtttca ttatcagatt
     7081 ctttcaaatt tcattcttta tcttatttca ggattttcta tgctgatttt actgagatat
     7141 gtcaatgttc gaaattgggt aagtgtacca gtagccgttt tattcatgag tacgtattct
     7201 attcaatact ggtctatcaa ccaaggattc actagctggg ggacttgttt ttatccttta
     7261 tgtatgatcc ctattgctga tttcgtcctt cataaaaagt tccctgttat caaagtcgct
     7321 gtctcagtgg gacttatgac acagatccat ttactgtctt cgatcatgtt agctttgatg
     7381 tatatccctt tttatcttta ttactttttt aaccagactg aaaaaggaaa agcactgctt
     7441 caactgatta agtcggtctt gctttatttt gttctgacag caaatatttg ggcaggatta
     7501 gctattgttt acagtgggaa taagattctt cctccctttg ttaacagaga aatgagtcaa
     7561 aaggcgatag atctggatgg cagttattgg ctccattacc ctaaggcctt tcctatattg
     7621 atattatcag gttttttgct ttttattttc tttcaacaaa agaacacctt attccaaaaa
     7681 atcgtgttgt acacaagtct tgttttcttt tttctttcta ctagcttgat tccatggacg
     7741 acattgatcg aaaaaaatgt gccttttatt agtttgatcc agtttccatt tcgatttttc
     7801 gtgccattta cgctattgct tctactttac cttgcccttt cactgaacca gtggtctgaa
     7861 caaaagtggt tccgtctttt aagtatattt ggcattctta ttttttcagc acagacgatc
     7921 cagaacttat accagcatct ggaaaagtgg gaaaatgaaa catttgtcag tcgacatact
     7981 tatctattcg atacgccgga agaagcaagg aaaacattct tcagcaaaga tatgtctacc
     8041 agcttgttcg ttttccaaaa aaccacacct gactatctgc caatctataa ggagacaaaa
     8101 gacaataaat acgaccggta taacgaagaa atactagaaa acgaaacaac ttttataaaa
     8161 acacatccag gcggtctttt gacaatcaag tggaaaaaca ctggggacaa aactgttcat
     8221 atccctgtta tcgtctacga tcggactgtg ctacagcaaa atggaaaaac attgactgat
     8281 tatgaagtaa cagatatcgg gaccccaatc gtgaagcagc aaaaaggaat caacgagctc
     8341 acgcttcatt atcaaacgcc aatctatttc tatttcattc tttctcttac tttgattggc
     8401 tggtttacct tgctatgttt atttatttat catagataca agcttttacg tgcttaggca
     8461 aaagggataa atacatccga tcaaaaaact ctccaaacgg tataatcgtt tggagagttt
     8521 tattttaatc agttttttta tgttatatgt tatatttttt attcgttcac atctaattcg
     8581 atttccaccc cgtagtgatc actgatgatt ggactatcga tcccatcaaa aatccgttga
     8641 tattttttaa cctgcattcc ttcagggacg aaaataaaat ctatacgcaa tttttctgta
     8701 ttttgttccc aaccatctat tttcttttcc acagtagctg cttttccttt ttcattcgcg
     8761 acaacatacg aatcttgaag aagaaggcaa ctttctctta cagtataata accttgagag
     8821 tctactggat tattgaaatc acctaggaaa aaaagtggta cgcgagtttc caaaaagtag
     8881 ttttccaagt ttttccactc aaaataaaag ccactttttt tatctgtcca ccaagaaaag
     8941 tggcagctcg agacaaaaat gtcctgatca tctatctttg ttttcccaat caagatcttg
     9001 cgtgtctgat aatcttcttt cttttgaaat tgggagacgg taaggacctg actttccaat
     9061 gtttttttcg taagcagtgc atttccttct tcatatttat cataaccgat gtggctcatt
     9121 tcccaactcc aaaaatactc ttttccgtgt tcctttaaaa gctgaactaa acggtaggca
     9181 aaattatctt tacggatagg tacttgattc tttaccggac agaacttcat tagcttcgca
     9241 ggttctactt cttcagattc cagtaattgg ttgatttctt gtaaagcaat cacttcataa
     9301 tcttctaaca ggatcttatc tgctaattct tgcaattttt tctctggttc tggctcaagc
     9361 cagctatgcg tgttgagtgt catgaatttc attggttttc tccttgtctt catttcgaaa
     9421 caaaagggtt gtgacaaaat ttttgtcaca acctctccag caaatagtgt tctcctgcta
     9481 actcctcagt aataagctca atggaccagg agctattttt tgtttggctg tttttatcac
     9541 ttggtgcagg gcagcttttt cacgacctct ccactaaaca gtattcaaac tgctgcttct
     9601 cggtaataag cccaaccaaa cgaaaaatag gaggagctat ttttgtttgg ctgttcttat
     9661 gacttgatgc aggacagctt tttcacaacc tctccttaca ttttaatgga gccgatcact
     9721 ttaccagctg tcactgtacc taattgcttg atcactactt cttctacttt atcgtttgta
     9781 aatacgatca tgatcgttgg atctttttct tcgttttgga tcacatcaag atccatctca
     9841 gctaatttcg ttcctgttcc tacttcttgg ccttctttga cagcaatctc aaaagctggc
     9901 tgattcattt ggactgtatc gattcccata tggatcaaaa cttcgatacc atctttcgtt
     9961 tccagcccaa ttgcatgttt gctagggaaa atacttgtca cttttccagc gatcggcgca
    10021 acaacttctc ttgatgttgg aataacagca aagccgtcac ccatcatttt ttgtgagaat
    10081 accggatctt ttacttcaga taaaggaatc acttctccag aagctgcgga aactagttct
    10141 tgttctatac ctaagtattg cttactacct gcttgtccat ttcctgtaga aaccatttct
    10201 ggctctggaa tctctgctcc tgattgcagc agatcttcga tatctgattt caagacatct
    10261 gcttttggtc cataaactgc ttggactcca ttgtctttga cgatcaatcc catcgcccct
    10321 gctcgtttcc aggcttcttc agtccctact ttttctcgat catttacact tacgcgtaac
    10381 cgagtcatac aagcatcgac atctttgata ttttcttttc ctcccagcaa atgaatgatc
    10441 tggaccactt gctgatctgc tgttcctgtt ccgctgttag atacaacggt ttcttcactg
    10501 tcattgtcgt aatttccgtt acgtcccggt gtagcaaagt tgaatttttt gatcaagaaa
    10561 tttgctaaga aatacgtaac aacaccaaaa gcgatcacac acaacacgaa gttgataaga
    10621 tctccaccta gacccgcttt gatagctaac ggtgtccgag tcagcaattc gatattccca
    10681 aatgaatgga cccgcaatgg caagatatct gccatcgcaa atgcagcccc ttggatcact
    10741 gaataaatga catataatgg cacagcagca aacataaaca tgaactccaa tggttctgtt
    10801 acacctgtta gaaagacagc caacgctgct gaaaaataca ttgatttata tcttgctttc
    10861 ttatcaggat cgacattacg atacatcgca aaagccattc ccatcaagat acctgaagaa
    10921 ccaatcattt gtcctacttt gaaacgagcc ggcgtccagt tatgcaacac aaactcatat
    10981 tgcgacatat cccctgcacc tttaagattc actaaatcag tggcccaagc caaccataac
    11041 ggatcttgcc caaatacttg tgtccccgcc tgtgcgcctg ataaaatctc atacgatccg
    11101 ccaagctgtg tatagttgat aggaatcgtc agcatatgat gcaatccaaa tggcaatagt
    11161 aaacgttcta atgttccaaa taaaaacggt gctagaatcg gtgcgctttc ttgtgattct
    11221 gcaatccata aaccaaaatt attgattcca gcctgtacgt ttggccacac cacagcaaga
    11281 atcaatgcca cgattgttga ccataagata acaacaaacg gaacaaagcg ctttccattg
    11341 aaaaatgaca aagcatccgg caattttcta taattatagt acttattata agccattgcc
    11401 ccgacaaatc cagcgatgat ccctacgaat actcccatat tcaaagctgg tgcttccaat
    11461 acacttgtaa aaaatccttt aaccatgatc ttcgtaccga acagagtgtg ggtaaatgca
    11521 tcttcattcg caagcatgtc acttgtgaca ccaaagatag ccccagtgat ccgattgatt
    11581 aaaataaatg agatcccagc tgcaaatgct ccgcctgctc tttcttttgc ccaacttcct
    11641 cctattgcta aagcaaataa aatatgaaga ttaccaataa ttgcccaacc aatattttca
    11701 attactccgc ccgttgtgac aagcggcgtc caatcaggat tgatcaaggg aattgatttc
    11761 cctatactga tcatcaaccc agctgccggc ataactgcta ccacaaccat taaagccttt
    11821 ccaaattttt gccaaaactc aaaactaaat atctttttca ttccgtttag ctcctttacg
    11881 caatcggttt cgttaaacgt attatagcgt gcgttttctt ttttgtaaat gctttatttt
    11941 ttatttttct gtctatacca ttctataaaa cctataatac gtgtgttccc ttctataatt
    12001 ttctcctaaa aatttatttt tttaagcttt tttctatgaa atattttctt ttgtctattg
    12061 acttgtgcaa tcggttgcgt ttattatgaa cgtataaaac agctcattag gaggagaaaa
    12121 atgaaacaga tcaaacgact atttgctatt gatccatgga aaatcagcac gacacaatta
    12181 gataaaaaaa atcttcgact gcaagaatct ttaacaagta ttggaaacgg ttatatgggg
    12241 atgcgcggga actttgaaga aacttactca ggggaccacc atcaaggtac ttatttggcg
    12301 ggtgtctggt atcccgataa gacacgtgtc ggctggtgga aaaatggtta tccagaatac
    12361 tttggaaaag tgatcaacgc cttgaacttt atcgcgatag atatttcgat caacgatcag
    12421 ctgatcgact tggcttcttt ggaaccagaa gacttttact gggaattaga tatgaaaaat
    12481 ggtgtattat ctcgtacatt cacaatcact acttctacta ataaagtacg catttccttc
    12541 gaacgttttc tgagcattgt caaaaaagaa tcagcttata tctatttaac tatagaaatg
    12601 cttgaaggta gcggaacagt caaactcgta tcaaaattgg atggaaatgt tcaaaacgaa
    12661 gacagcaact atgatgaaca tttttggaat gagattgatc gtggaactat agaagaaatc
    12721 ggctatttga cgactaaaac tattccaaac aactttggga ttgaacaatt tactgttact
    12781 gcagcaatgc gccatttcgt taatggaaaa tcagtcatcc ctgactatca cgaagatact
    12841 ttagcattac atggttgctt gtctgcagaa ttatctcaag gtgagacatg gtcagtggaa
    12901 aaagaaatta tcgttcttac tagtagagat attccagaag cgcagcaagc agaaaaagct
    12961 gtctcagaat taacgaacca gacaaacaat tatatccaag caaaagaaga gcaaacagcg
    13021 gcttgggcag aacgatggaa gcttgccgat gtggtcatcg aaggagacga agaagcacaa
    13081 caaggtattc gattcaattt gttccaacta ttctctactt actacggcga agatgaacga
    13141 ttgaatatcg gccccaaagg attcacagga gaaaaatacg gcggagccac ttactgggat
    13201 acagaagcgt atgctgtccc tctttatctt gctttagcca aacctgaagt aactaagaat
    13261 ctgctgaaat atcggcataa tcaactgccg caagcaatcc acaacgctca gcaacaagga
    13321 ttaaaaggcg cgctttatcc tatggtcacc ttcactggtg tcgaatgcca taacgagtgg
    13381 gaaatcacat tcgaggagat tcaccgaaat ggcgcgattg cctatgcaat ctacaattat
    13441 acgaattaca ccggcgatac ggaatatttg aaaaatgaag gactggaagt tttagcagaa
    13501 atcgcacgct tctgggcaga ccgtgtccat ttttcaaaac gagcaaataa atacatgatc
    13561 cacggtgtga ccggcccaaa cgaatatgag aacaatatca ataacaattg gtatacaaat
    13621 acgatcgcag cttgggtctt gcgttacacg cgagaaaatt atttaactta tcaaaaagaa
    13681 gctgctatca ctatcagcga agaagaacta gccaaatgga aagatatcgc agagaacatg
    13741 tacttcccag ttgacgaaga attaggaatc tttgtccagc atgacacatt cttagataaa
    13801 gatctcatgc ctgtatccga tattcccaca agcgatcttc ctctgaatca gcattggtct
    13861 tgggacaaaa tccttagatc ttgcttcatc aagcaggcag atgtactaca ggggatttat
    13921 ttcttcaatg atacttttac aaaagaagaa aagcaaaaaa actttgattt ctatgaacca
    13981 atgactgtcc atgagtcttc cctttcgcca agtatccatt cgattttagc agcagaatta
    14041 ggaatggaaa gcaaagctgt agaaatgtac cagcgaacag ctcgcttgga tttggataat
    14101 tacaataacg acacagaaga cggcttgcac atcacttcca tgactggtag ctggttgtcg
    14161 attgtccaag gatttgctca aatgaaaact gcccatgatc agttgagatt cgcaccattt
    14221 ctgccaaaaa cttggaaagc ttattctttc catatcaatt atcgcggacg tttattgttc
    14281 atctcagtca cagatgaaca agttactttc gagttattag aaggttctcc tctagaaatt
    14341 tacgtctatg aacagtctta ttctttagcg gatcgtctag aaataacgct aagaaaggag
    14401 gaagcaaatg tttaaagggg tattgtttga tttagacggt gtcattaccg atactgctga
    14461 attccattat cgtgcttggc gaaagcttgg tcaggaaatc ggcattacga tcgaccacac
    14521 attcaacgaa cagctaaaag gcgtcagccg agaagactca ctctctttgc ttttagctta
    14581 tggcggaaaa gaacactctt tttcaaagga agaatttgca gaattagcga aaagaaaaaa
    14641 cgattattat ttagagatga tccagacgat cgaaccaaaa gatgtcttcc ccggggtcgt
    14701 gcctttactg gattctttaa aagaagaaaa aatcaagatt gctcttgctt ctgcaagtaa
    14761 aaacgggcca ttcctacttg aaaaaatggg tcttacacct tactttgatg ctattgccga
    14821 cccggcagaa gcaacaaacg gaaaacctgc gccagatatc ttcctccttg cagcaaaagc
    14881 tgtcggacta aaagcagaag aatgtatcgg gatcgaagac gcgcaagccg gtatccaagc
    14941 catcctctca agtggcgctc aaccaattgg cgtgggacgt gctgaagacc ttggagagga
    15001 gatcccgctt gtccctgata cgacttttct tacgatcgat tacttgaaag aaaagtggca
    15061 tgaccatgga taatccagag atctccgtcg taccagttcc agagactgaa ataaagaaaa
    15121 tcacattgaa gaacgactac ttagaagtcg ttcttctcaa ttacggtgcc cgcctgcacc
    15181 aaatcttcgc accagacaaa gaaggaaaat cagaaaatgt tttattgtct tacgatcgtt
    15241 tcgaagatgt tctaacagac aaaagctttt ttggagcaac tgtcggacct gtagccggaa
    15301 ggatccggga tgcttcttgg ggtgagcata gattggagaa aaactgcgga tcacaccata
    15361 tccacggtgg cacgaaaggc tggtctttcc aattttggga agtagaagtt ttcaaaactc
    15421 catcatcaat aggtgtggtc ttttacttaa aagatgaatt ttctacctat ccaggaccaa
    15481 tcactgctac gatcacttac cgtttaacaa aaaacgaatt agagatgaca acctctgcta
    15541 gtagccttgt tgaaaccatc tgcaatccga ccaaccatgc ctacttcaac ctttcaggaa
    15601 atggaacaag agacatctat gaacatcagt taacggtctt tttagatggt gtgcttgaat
    15661 tagatcaaga aaaactgcct actggaaatt ggacaaaaaa agaaaaactg ccaatcgact
    15721 ttagaaaatc cccaacactg caagagatcc ttgcatgtta tccagatgga ctagatgatg
    15781 tatttctttt gcatcatcct aggctttcca aaacttcctt acaattattc gagaagcatt
    15841 ctggtcgcca aatgactata gcaacttcta ataaaagtat ggtattattt tctacgactg
    15901 gattcgaagc tgatttctct gtcaatggaa aacagatgca ttcaaactac ggattagcaa
    15961 tcgaaccgca agaaatccct gatatcgttc atttccccaa atttggatcg atcaacctcc
    16021 atcctggtca agaacggatc agtcagacga tttatcggtt ctctgcacaa taacaagaga
    16081 aaggtgtttt tatcatggct attaccgtaa aagatgtggc gaaaaaagcc ggtgttgcca
    16141 cctcgaccgt ctctcgtgtc atcaacgatc atcctagtat ttccgagaca acaaagaaga
    16201 aagtccgaaa aattatggat gaactaggtt atgttcctaa catgactgcc cgcaatctag
    16261 gaaaacgcat ttccagtgcc atcggcgtaa tcttaccccc tcttgattca aaagagcgga
    16321 tcggtaaccc attttatctt gagatcatgg aagcaatcaa cgaagaagcg cggaattatg
    16381 atatgactac agcaatcgct actgctaaaa attttgaatt gctgttagag aacgtccagc
    16441 gtatgcattt acaaaaacaa gtagatggtt ttattctagt gtattcagat agtcaagata
    16501 ctgtcattga ttatttatac caaaaccaca tccctttcac tttgatcgga cagccttatc
    16561 atcatgagaa cgaaatcgtt tatgtagata atgataacca gcttctaggt aaacaagcaa
    16621 cagaattttt gatccagaat ggacataaga atatcctgtt tgtgacaaac acgactcatg
    16681 aaagtttgta tttcgaacga tatttcggtt atcaaaaagc aatgatgatg gctggtcttc
    16741 ctgcgttatc cagtgtagta ctagagcatc cggaggatta tctgtccttt gagaaaacgt
    16801 tgaaagaaaa cgaagccaca gcattggtgg tcattgacga tatcttcgcc cttcgcacga
    16861 tgcagttggc gcaaatgtat ggctatgtca ttccagagac actctccgtc attagcttca
    16921 acaactcgat cttctcaaca ttaacacacc cttaccttac aagtatcgat atcgatatct
    16981 ccgaattagg acgtgtcgct atgcaaaagc tgcgcgaatt gatccatgaa gaattgagca
    17041 gtggcgtacg tttggtcatc cctcatcgct tgatcaaaag agagacagtc gttcctttag
    17101 aaaacccata ctaaatagct gactcactaa atacgctgac catttatcct atggtcagct
    17161 ttttatgtaa aagaagatac tacttctaac acattgttta tctgtaaaaa agagcgtggg
    17221 acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaagcagaa
    17281 gcaactcctt cggaaataag ctgaaattca caaaaattta aaaagcaatt ttcttgagtt
    17341 tcttcttatt tcttggagtt aaacgcttct gtcccagcct cttttttcct tccttcaatt
    17401 ttttacacgt cgacgattca ataaaaaacc gcctaatatt gtaaagacag gtgcaagaat
    17461 cgagaaaaag gcgaatggca agtaatgcaa cgcgctaact ttcatcgttc ccataatgaa
    17521 tgtgccgctg actccccacg gtacgagaga attgacggtc gcacctgaat ccgctagtgt
    17581 tcgtgtcaaa taggtttttg ataagccaac gtcatcaaaa gaacgcttga atgtttctcc
    17641 tggaagaatg atggacagat actgttctcc aacgatcaga ttgattccga tacagctcaa
    17701 ggcagtaaac ccgactaaac gagaaggtcg gtccatcttt tcttttaatt cttgaacgat
    17761 ggtatcaacg atcagatatt tgatcagtaa cccacctaat gccaatgcaa gtaaaatcaa
    17821 actagcagaa cctaacatac tcatgatccc gcctcgagaa agcagttcat ctagctttgg
    17881 atcaggtgta tttgcgacat aaccattcat caagatgtct gctgcttttc cgaccgtcaa
    17941 acttttctca tggataaaag aaagaacgag agcagctgtc gatcccgcta gtaatgtagg
    18001 gatagccgga atttttttga ttgccccccc aaataaaata actagtggaa tcaatgtcca
    18061 aggagagatc caaaagctcg ctttcatgtt ttggatcatt tcattgatac tagctagatc
    18121 ggctgaatga ctgttatgtc ctaatatcgt ataaaaaatc aacgaaagga cgaatgctgg
    18181 gaaatccgta taacgcatac cagcgatatg ttcgaataga ttaactcctc caatacccgc
    18241 tgccaagttg gtcgtatctg ataaaggcga gcagttattc cctaaaaagg caccggaaac
    18301 gactgcgcct gtagtcattg catcattgaa tccaagtaaa tgaccgatcc ccaaaaaagc
    18361 aattccaatc gtcgaaatcg ttgtaaatga actgccgaca gtcactccga caattgcaca
    18421 aacaagaaat gctgtcggta aaaagaaacg cacagaaaca agtttaaatc cataaaccat
    18481 aatcgtaggg atggttccag ataaaatcca tgaagcaact aagacaccaa tcaaaagaaa
    18541 aatcaggatt ggaatgatcc ctggagtgat tcctttgatg atcccgtcat ggatctcatc
    18601 ccatgtgaac tttttaaatc tgccataaaa caacaaacac atgaaagctg caaggatcgg
    18661 gatatgtggc gagagctgga atccgatgat cagtactcct aggagtgcca acaacacgat
    18721 caatacagcg atactttctt ttaaagaaat atttttattc atcgtcgatc tcctatttca
    18781 aatgaaaagg ccctgccatg aaaaaactat tcacttggca ggacctccta ctaaatcagc
    18841 gacaaaacgt cggctgaaag acttattttt ttatttttct tctaaacttt tttctacctc
    18901 aatcccccaa gaatctttct tgaagtgatt gatgatcaat ggaataacga acatgaccac
    18961 tgtgatagct gctactaata atgcatcact caatgtcgct gtacctaatg tagcaaatac
    19021 gacaacgatg atagagaaga tcagcatgca gctgactacc caagctaatc cgtttccgtt
    19081 tttaccaatt cggtaaggac gttcgaattc tggatgtttc ttacgcattt taagcaatga
    19141 aacagcaatc aaaatgtaca cgatacaata aataacagtt gtagcatttg taagtgtcaa
    19201 gaatacagca cttacgtcat ccattacacc ataaagcaaa gcaaataatg aaataacgat
    19261 tgattgtgtt agaacgacat ttctagatac gccgtatttg ttttctttat ggaatccgaa
    19321 ttttggtgga aggaatcctt cacgagctac ttggatgatt gtttttgatg gtccagtaac
    19381 ccaagctgat aattgtaaaa gcacaccaat aaataccatg aagctgaaga tatttccgat
    19441 cacttctggt aagcctagga tttgacagtc aatcaagata ggctgcgtga tatttgctaa
    19501 ttccattttt ccatctggta cgacatttgc tacaagcatc gcattgatga cattcaataa
    19561 aactaatccg atcaaagcga taaatacgcc ttttgtataa ttttttgttg cgtctttcaa
    19621 acgaggcatg taaacagaac tcatttcgat cccaacaaaa ataaatgtga taccagctaa
    19681 gtatttcaat gaatcaatat gttccaagtc agggaaagct tttgaccatg aaaagtcacc
    19741 tagatagccg tttgagacta gtccgacttt gaaggctgcc aaaacaccta aaacgaacat
    19801 gatgactact gggatatata cccctagcca tacaccgatg ttcccgccga cttttgccat
    19861 atcgaatttc aagttcaaga tcgtgatgat ccagtagata actaaaatac aacctaaaac
    19921 gaaccagtga ttattgccaa gttctacatt accaaatgtg tttcctaaca atggtcctaa
    19981 agtagaagcg accattacca tacctgggaa catttgcacc cataatagcc atgcaacgac
    20041 aaatccccat ttgctgccca gtgctgtttt tacccataac tgaggaccgc cttcttgagg
    20101 aagcatcgtg gaaagctcac cggaaaccat tgatatcgga ccggcaaaaa acaaaactgc
    20161 aaaaattgaa taaaaaatca atgtccaacc gactgcggct aacgtaggaa tactccgaac
    20221 cgttgcacac agtgccattg tcagaccgat aaaggtccca agacccattt tacttacttc
    20281 agatttactc accgttgaca ctctccttga ttttttggat aaaataacgg tgtctttgaa
    20341 gtagtttcag caataaacga aacaattcag cgaatatttg aggggattct ttgagtttat
    20401 tgcttgaagg gtttcttcaa agacaacctc aaaacaaagg gatcgaagag cttaggtctc
    20461 cccttgctct tcagatccaa acatgcgtca gaaacagtta gtattcaagc tgttttctaa
    20521 catgattacc ttgcttattt tgcttcgctt gccaataatt ggtctgcgta gattttttcc
    20581 aatttttctt gaagagctgc tttgattttt tcagcatatt cttcaaagtg ttcttctttg
    20641 ttcatgtatg gtgtcattac gctggcacgc aatacagtta cttttccggc acggttccat
    20701 tcttcatctg agaatcctag ttggttcaca aattgtaatg ggctgttgcc atagtctggg
    20761 atagcaaagt cagtatgtga agttaagaac tcattaccgt aaatgcttcc tttcacgtat
    20821 gaagaataat catatacgtc atggttcaat ttattcattg caactaagtc atcgttgcct
    20881 ttttctttga atacatagtc taccatattg aaatctggat aagttaatgg atgaacttcg
    20941 atttctttgt ctcctacttt aaatgacaag tcattcaaga agttgtagaa acgatgtgca
    21001 ccttcgattg atgcacccat taatttacca tatcctgtga cattcaaagg taagacatgg
    21061 tgtgctgccc aaacgctggc tgctgttgcc cctgcttttg aaccttccaa gatataagcc
    21121 ccaagtaatg ctgggatatc tgcgcctttt tcaaatacat atgtcgcaaa gtaagagata
    21181 acgtctctca tgcggatatc tttgatgacg ataccaccgg cagaatatgg aacatagccc
    21241 attttatgtg ggtcaatcgt tacagattct gcttcttcga ttgctttata tgcactgtgt
    21301 acttcttcta agatataatc tttgttttct gtaaagacat tgtatttgta atgaacatct
    21361 tttaaatctt caaatggaat gaagttgttg tcttcgtcta agaaaatcgc acggccgtat
    21421 ccgccataag cagcatctac gtgtaagtag aagtaaatac cgtctttttc taatacgcga
    21481 cgcaatgcaa cgattttatc gataccgtcg attgctcctt cttctgttga tcctacaaca
    21541 ccgactacac ctaggattgg tgttttttca gctgctagac cacgaacgat tttttcaagt
    21601 tcgttgatgt ccatgcgata gttgtggtca actggcactg gaatcacttg gtctaaaccg
    21661 ataccgatga tgtcggcagc ttttagccat gaatagtgtt tagtttgtgg aaccaaccat
    21721 tttcctaatt tttctaaatg ttttccgcta cgtgctgagt gagctttgat ttcgtcgatt
    21781 ttttctggga cgctgtctaa cagattcatg atttcttctg ttgacaagtt cattaattcc
    21841 caatcgcttt ttccagcaac tagttcaggt gtcacttctt tcattgcaag cggcaatgat
    21901 ttgatgttgc gggcatacca aagtccttct aagtttgcta aagaaccatc agcaacgatg
    21961 tggccccagc catctttata gctcattaat tttgcaaatt ccattcctac ttcttcttcc
    22021 atttggcttg ttgcaggaga agattcatag gcaacgttat taccattcca tagcatagca
    22081 aaattgtacg ctagtaaaga tggcatcaat gtttcagagt tcatatgacc ccagtaacgt
    22141 ccggcattat gccaaggaac ggaatgggta cgcatccgcg ctgaaatttc tgatagaaca
    22201 tcctttgttt tattcacagt gtgttcaaaa ctagcactgc ttttttcttc tggcgtaata
    22261 ataggcatgt cttgaggcat gtagttttga cgccagccta aatgttcatc caccaattca
    22321 ttcaacaaag ctttatagat ttggccgttc tcagctttgt ccccaatgaa tagggcattt
    22381 aggtttagat ctttcgacaa tgattcactc atagtgaaac cctcctaaaa aaatatttaa
    22441 attttgtcga gccctacatt acacctgttc ttctattctg taaactaagt tttggcctga
    22501 tttttaatca tattttaata tgtatcaatt atccgaattg ttcacacttt tgaaataatt
    22561 aaaaaaacga gggaatttac aaatgtaaac tccctcgcaa agattgtatt aacgacaacc
    22621 agcgttctat tttaggatgg gtttcaaaat tggaatgttg taatagtatt tccctttttc
    22681 aaggtaaaaa cttactaaat tctaatcaat tactttggcc aaaaagtaat ttttcttacc
    22741 ttttcgaata accacaaact taccatcgaa ttcatcggat ggatttatga caaaatttaa
    22801 atcggtaacg cggtcaccgt taatacttat agctccgttt gaaacatctt cacgtgcttg
    22861 acgtttagat ggttcaattt ttgtggaaac aagcagttcc acgatatttt caggtgtgct
    22921 tgaaatttca acgttcggca ttttaccgaa tccttgcgcg atttcttccg catttagttc
    22981 tttaatattt ccagagaaca atgcttgcgt tattttttgc gcttctttca agtcttcttc
    23041 actatgcaca aatcttgtaa cttcttctgc caaacggcgt tgtgcttcac gtttctcagg
    23101 ttctgtctct acttttttcg ctaagtcttc gatttcttct tgagacaaga aagtaaagaa
    23161 tttcaagtat ttcactacat cgcgatcatc ttgattcaac cagaattggt agaactcaaa
    23221 tggtgaagtt ttcttcggat caagccaaac ggcaccgcct gcagtttttc cgaatttcgt
    23281 tccgtcagcc ttcagcatca atgggatcgt taaaccgaac gcttctgctt ctggtccttc
    23341 taattttcgg attaggtcta atccagctgt gatatttccc cattggtctg ctccaccgat
    23401 ttgcaattgc acatcgtatg ttttatgcag atgcaaaaag tcgatagatt gcaggatttg
    23461 gtaagtaaac tctgtaaatg agattcctac ttccaaacgg ctggcaacga tatcttttgc
    23521 taacatcgtg ttgatgttga agtttttacc gtagtctctc aaaaattcaa gtaaagagat
    23581 ttttgacaac cagtcgtagt tgttcacaaa tgtgatattt gcatctttac caaacaattt
    23641 tctcatttga ttggaaagtg cgcccacgtt atgttgcact tgttccatcg tttgcaacac
    23701 acgttctgtt ttgcggccgc ttgggtcgcc gatcgttcct gttccgccgc caatcagaat
    23761 atatggatga tggcctgcca attggaatct tttcatcatc ataaatggaa tcaaatgtcc
    23821 gatatgcatc gaatcaccag ttggatcaac gccgcaataa agtgaaatgc tcttttcttc
    23881 aacgagctcg cgaagtcttt cttcgtttgt ttgctgatta atggcgtcgc gccaagttaa
    23941 ttcatcaata atattcattt tactgacctc cgtgtagttt ttttatgaaa gcaggaacct
    24001 gctagcatgt gtaattaatt atttcttcag gataggatag gaaataaaaa aagtcccttg
    24061 gcatataagc caagggacga aatctcgcgg taccacccaa tttgtatgtc tagatatcca
    24121 acatacctct cgaacattta acgctgtcgc gtggcaatca ctgccaaaga ctcctgaaat
    24181 gtaattcgct ttaaagcatg tactggtttg cagcgaccac cagctttctg aaacattggc
    24241 tttaacaact acttcgtttc gatcttcgtc ttgttattat taatatgtgt ctatcataga
    24301 gcgcaacttt ttctttgtca acagaaaaag gatatttttt ttaaattcct cattttttct
    24361 tttttgaatc cgctctaatt ccgaacattt tattttcaat tattgttaaa agaaataact
    24421 ttttctgtat tttttctgca atttatcgag aagtcacttt actttgcctc ttattctttc
    24481 ttcgttacaa taaggaatgg gacaagtaaa aaaatgaaaa agcaagggac gaggtatatg
    24541 gaaaaaaagc aaaaaattat tttaggagcc acttctgctt taactatcgt tattttaatt
    24601 ctaagtttgt tttttgtcat ccgatcatct acaaaaaaag aaccaatcat tcctgaaaaa
    24661 aaaataacag aaaaagcaaa agaaccgaca gatgataagc aacaactagt tactcaagca
    24721 aaaaatctgg ctcaaagcta cgattatgac caagcgattg cgttattgga aaaagatagt
    24781 aagaacggat ctactgaaac acaacagctt cttcaaacac taaaaaagga gaaagaacaa
    24841 ctgatcgact ggtctgatcc tactcagatc tctcatgtct ttttccacag cttgatcgtg
    24901 gacccgcaaa aagccttcca ctctcaacag gcacaaggat acaaagatta tatggtgact
    24961 gttgaagaat tcaaccgttc gatcgaacaa ctttatcaaa atgattttgt tttagttaat
    25021 ttgaatgatt tgatccaaaa agacgaacaa ggaaatcttt cctttaacgg gcttgcgcta
    25081 cctgaaggaa aaaaaccatt ggttctttca caagatgatg tcagctatta tgaatatatg
    25141 gataattccg gttttcctag ccgattggtc gtatcaaata acaatgaaat caaaaatgtc
    25201 tataaagaga aagataaaga agaaatcggt gactttgaca tggttcctct gatcgatgcc
    25261 tttgtcaaga aacatcctga tttttcttat cgaggcgcaa aaggtacctt ggccctgaca
    25321 ggttacaacg gagttttagg ttatcgaaca tcaaaatctc aatatggaga caatgaaaaa
    25381 acaaaaaaag agattaaaga agctaaaaaa gtggcggaac agttgaagaa agacggctgg
    25441 agctttgctt cccatacatg gggtcacttg aatatgactc aagcttcact agaagatgta
    25501 aaaaaagaca atgaattgtg gcagcaagaa gttgcaccta tcctaggaaa aacaaatatt
    25561 ctgatttatc catttggtgc agacatcagc gactggcagc cttattcagc agaaaatcaa
    25621 aaatttgctt acttgaagag tcaaggtttc gatattttct gtaatgtcga tgcttctaca
    25681 ccagcttggg gacaattagg tacggatttc tatcgaaatg ctcggatcaa tatcgatggc
    25741 attcgttttc aatctgattt agacggaaaa aatcctattc ttgatccatt catcaatgtc
    25801 catgatgtct atgacaaaga agcacgcgaa tctgcttgat tttacgtcat ttatgaacca
    25861 aaaaagccga actgacattt tatcgtcagt ttcggctttt ttatgcttta tttcttcttc
    25921 ggtgcgattc ccttgatcag gacttctgtc cattctgctg tatatggaag cagttttgcg
    25981 tatatctccg gcttagaaat tccttctacc attgcttgaa aatagaggat caagaactca
    26041 tccgaatatt tcaaatccac ttttccttct ttccgccctc gatggaaaag atcaagcatg
    26101 atactgaaac tttcttgcgt atacgcatcc atcgcgtcag aaaattcttt tttttctttt
    26161 tttgtatagt atgtcatcaa atcgatataa aattgtttgt tcactcgatt gagataacgg
    26221 attttgtttt cactcatggc aatcagtgtt tcttcaaatg gaagattttc cgcaatcaac
    26281 gctttcgctt cttgccctaa atcattcata aatgcaaaaa atacttcata aatcagccct
    26341 tccttgctgc caaaatattt aaaaatcgtc gctttactta cctgagcgtt ctcagctacc
    26401 ttatccattg tcactccagc ggttcctatg tctgtgttca tcaagtcaaa agctgcttgt
    26461 aaaatctctt tctttttctc ttgtgctctc ttttcaaaac cgttcatttg tagagcctcc
    26521 cgtaatttta aacttttata atatatatag ttcaaattta ttctgaggtt tattataact
    26581 atctattgag aaagtatcaa gctagcaatt ccttgtttat gaatt
//
LOCUS       DAHHZJ010000024        24384 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.025, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000024 DAHHZJ010000000
VERSION     DAHHZJ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.025"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(185..2266)
                     /gene="cadA"
                     /locus_tag="NBL02_000805"
     CDS             complement(185..2266)
                     /gene="cadA"
                     /locus_tag="NBL02_000805"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HCD2571511.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            complement(2791..3150)
                     /locus_tag="NBL02_000806"
     CDS             complement(2791..3150)
                     /locus_tag="NBL02_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCD2571512.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(3180..3521)
                     /locus_tag="NBL02_000807"
     CDS             complement(3180..3521)
                     /locus_tag="NBL02_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571513.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(3518..4192)
                     /locus_tag="NBL02_000808"
     CDS             complement(3518..4192)
                     /locus_tag="NBL02_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HCD2571514.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKSLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(5244..6542)
                     /gene="asnS"
                     /locus_tag="NBL02_000809"
     CDS             complement(5244..6542)
                     /gene="asnS"
                     /locus_tag="NBL02_000809"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCD2571515.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(6582..7772)
                     /locus_tag="NBL02_000810"
     CDS             complement(6582..7772)
                     /locus_tag="NBL02_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCD2571516.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(7793..8320)
                     /locus_tag="NBL02_000811"
     CDS             complement(7793..8320)
                     /locus_tag="NBL02_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HCD2571517.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGYESGDEVKTIKGI"
     gene            complement(8367..11156)
                     /locus_tag="NBL02_000812"
     CDS             complement(8367..11156)
                     /locus_tag="NBL02_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCD2571518.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKATLKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(11303..11503)
                     /locus_tag="NBL02_000813"
     CDS             complement(11303..11503)
                     /locus_tag="NBL02_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCD2571519.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(11955..12275)
                     /locus_tag="NBL02_000814"
     CDS             complement(11955..12275)
                     /locus_tag="NBL02_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571520.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHILHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(12778..13896)
                     /locus_tag="NBL02_000815"
     CDS             complement(12778..13896)
                     /locus_tag="NBL02_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571521.1"
                     /translation="MAKKDKELLSQLISEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FSKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            14369..15676
                     /locus_tag="NBL02_000816"
     CDS             14369..15676
                     /locus_tag="NBL02_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HCD2571522.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKVSGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANMFVIGWRRVAEFFGNLF"
     gene            15794..16993
                     /locus_tag="NBL02_000817"
     CDS             15794..16993
                     /locus_tag="NBL02_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCD2571523.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRVIFSSNNYHIF
                     RAGLYACQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(17020..18288)
                     /locus_tag="NBL02_000818"
     CDS             complement(17020..18288)
                     /locus_tag="NBL02_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HCD2571524.1"
                     /translation="MEKQLPSPMRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DTRLSHPWLLYLLPVSGALFAYLYVYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(18451..19098)
                     /locus_tag="NBL02_000819"
     CDS             complement(18451..19098)
                     /locus_tag="NBL02_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HCD2571525.1"
                     /translation="MEQTIKPYQYFFIKREVEQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILDHELLEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(19506..20009)
                     /locus_tag="NBL02_000820"
     CDS             complement(19506..20009)
                     /locus_tag="NBL02_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCD2571526.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(20132..20896)
                     /locus_tag="NBL02_000821"
     CDS             complement(20132..20896)
                     /locus_tag="NBL02_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HCD2571527.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLADVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            21003..21278
                     /locus_tag="NBL02_000822"
     CDS             21003..21278
                     /locus_tag="NBL02_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCD2571528.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            21874..22824
                     /gene="yidC"
                     /locus_tag="NBL02_000823"
     CDS             21874..22824
                     /gene="yidC"
                     /locus_tag="NBL02_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCD2571529.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(23003..23977)
                     /gene="lacD"
                     /locus_tag="NBL02_000824"
     CDS             complement(23003..23977)
                     /gene="lacD"
                     /locus_tag="NBL02_000824"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HCD2571530.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDTKMPGR
                     LPDLLNIWSVKRLKEAGADACKFLLYYDVDEPAEINVQKQIVIERIGSECEAEGIPFF
                     LEILSYDTTIGDPSSLAYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QETCYSQAEASAYFYEQSKVTEQPFIFLSAGVNATLFQDTLRFAKQSGSTFNGILCGR
                     ATWAKGVAPFVTEGEKKASSWMLEEGKQNIKELSTLLKETATPVYVDR"
     gene            complement(23974..24147)
                     /locus_tag="NBL02_000825"
                     /pseudo
     CDS             complement(23974..24147)
                     /locus_tag="NBL02_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
BASE COUNT         7000 a         5145 c         4315 g         7924 t
ORIGIN      
        1 tttttccatc aaaaaaataa gttattttct tttatttcat tgaccttatt gaatttcaag
       61 ctattttgaa ttttctgcaa aattaaaaac attagttatt ttataatcct atctattttc
      121 tttacatttt tcattaagaa aaggatataa aaaaaagagg acgttcattc gtcctcttca
      181 ttgatcaacg tttatttttt acatgcaagc ttcgcatggc attcaaaaca gctaatactg
      241 tcaccccaac atctgcaaat actgcatctc ccattgatgc aaaaccaaaa gcacctaagg
      301 caagtaccaa aatttttatc ccaatagcaa agacaatgtt ttgtttgacg attttcaatg
      361 ttttctttgc taaatggatt gcttctgcaa tcttcgatgg ttgatcattc atgatgacta
      421 catctgctgc ttcaattgca gcatcgcttc ccaatccacc cattgcgatt cctacatctg
      481 ctcttgctag caccggtgca tcgttgattc catctccgac aaatgcggtc tttttattgt
      541 ttctttgtaa gatttcctct aaccgctgca ctttatcttc aggaagcaat tcgctatata
      601 tcttgtccac gcctacttgc ttcccgatat gatcagcaat ttttttcgag tcgcccgtca
      661 acatgactgt gttcttcaca ccagcctgtt ttaattgaac caatgcgtct acggcatctt
      721 ctttcaaggt atctgcaata acaagataac cactgtaact ttgatcgatc gctagataaa
      781 gcaatgtacc gatttcttga cttaaaggaa aagagatttc aaatctttcc atcaatttgt
      841 gattacctgc taatacttgt ttgccatcaa cttcagctaa gatcccttca cctgcaaatt
      901 cctggatatt agttgttggt aaaagcggac ctgcgtagcc ttctaaaaca gattgagcaa
      961 tcggatgtgt agaaaactgt tctgcactcg caatatattg catgaatttc tttggatcga
     1021 tttttgtatc gatcgtctgg atcgaaaagt cacctttcgt taatgtgccg gttttatcga
     1081 ataccactgt ctcggtttgc gcaagcaact ccaagtaatt tgaacctttt actaatacac
     1141 cgattttact tgcgcctccg ataccgccaa agaaactaag tggcacggaa atcactaatg
     1201 cacatggaca tgagatcact aaaaatgtca atgctcgata gatccattca gaaaacgccg
     1261 ctccagaaac aacaagtgga ggtaaaacag ctaataaaac ggccaatcct acaacgactg
     1321 gtgtgtaata gcgcgcgaat ttagtaatga aattttcagc tggtgctttt ttactgctgg
     1381 cattttctac taattctaat aatttgctga tagtagaatt ttcataagat ttttctactt
     1441 gaactaaaaa tggtttattt ttattgataa atccgcttaa tactgtttca ccaacatata
     1501 cagatttagg gactgattct cctgtaagtg ctgacgtatc gactaatgat tctccttcga
     1561 tgatcgttcc atctaacggt acacgttcac caggcttcac aagtacaaac tgaccgatct
     1621 gcacgtcttc agggttcacg gtcatcaatc cttccgtcgt ctgcacgtga gctgtttctg
     1681 gacggatcgc catcaattcg cgaatcgatt ttcttgaatg actgactgcc aatccttgga
     1741 aatactcgcc gatttgataa aatagcatca ctgctacggc ttctggatat tcaccgatca
     1801 acatagcgcc aatcgtagca acagacataa gaaaattctc atcaaagatt tttcctttga
     1861 cgatattcat cactgccttc ttcactacat catatcctat cagcagataa atcaataaat
     1921 accctatcaa gcgaattggt tcattaaaat caaataatag caataaagcg atacctgcaa
     1981 gggaaaataa aatccggaac agcgtatttc tctctttttc atttccatgt tcagatactt
     2041 tttccttatc tatttctgtt actttgacat ctggttctaa ttccttcact actttttttg
     2101 tttcttgttc aatcgtagcc aaatgatctt cctctgcgtc aatcgttagt tttccagaag
     2161 tgaagtttac cgttgcttcc ttgactccat tgatcttctg aactcctttt tcgattttca
     2221 ttgcacagtt cgcacaatct aaaccttcta atctgtaact tttcatttta atgacctcct
     2281 cgcctcaaca tatgaatatc tcttcataac taattataaa tgaatggtta ttcatatgtc
     2341 aaggtatccg aaagaaaaaa tgaaaaacat ctgcatcgga aaatttcttc catacagatg
     2401 ttttggctta ttctttcttt ttctcttcga tcattgcccc tgctcttatc caacccgcac
     2461 ttgccttctt gatcttgaca ggatagcaac taagtttgaa gcctgttgct ggcaatttgt
     2521 ctaaattcat taatttttcg ataggacaat atcttttttc aatccctgca aaatgtcctt
     2581 cccagatatt cgaagctcca ctacgctttc aaaaatatag aaagagttct ttaatgaaaa
     2641 ttatttcaac tttccataaa cagtaatccc atataaataa aagacgtaaa agtcacatgg
     2701 gagaacttat acaggaaaac acatagagtc aggtgaaata catgtatccc acctgactct
     2761 atgcgttttc taacctttat tattctcttt ttatacaatt tctgcaccga atggcatcaa
     2821 cgccaatcga gcaagtttga aagattgctt ggcaaaaggt atccctatga tcgtgataca
     2881 tagaaggatg ccgctgacga catgtgcgag agcaattggt aggcctgaaa cgatcagcca
     2941 gatgatatta acgatcaatg acatgccaga ctggtgataa attacttctt tgccaaaagg
     3001 cgcaaaagaa agggaggcga tcttaaaaca ttgcataccg attgggatac ctatgatcgt
     3061 gatacaccaa agtatccccg ctgctaacca gctaagacca ccgattagtc cgccgaatat
     3121 aaaccagatg acatttccta aacaactcat tgtatttctc cttctattac tgtttttgat
     3181 taaaactggg caaaaattga ttttattctt ctttttttag cttccagcat caagtattct
     3241 gcaaattttt tttggatttc ttccgtattt tcttttgtta ctaatgtgtg ttggattagt
     3301 tttcctgatg gtcgctgatc cacccgttct tctgactgtt gcacgtccgc ttgcgcaaat
     3361 ccgataatcc gtttttcttt tgttacagca acgagcaata gtttattctt atcatttagg
     3421 tcagctgccg tttttgcttt tacgaatgat tctggatgat cagtaatcag ctgattgatt
     3481 tgaggtaaat cttcttttac tgcccaccgt atttccatca tttctcactt ttctttcgct
     3541 catttttatc tccactaaaa aaatcgactg cggcaattgc atctatccag ctttgtgcaa
     3601 tgatctcatg tccgccaatc gttgggtgga ccccatcttc tcttgtatat ttcgaaaaac
     3661 cataagcaat ccctaaagca ttgaatatgc catctaaagg aaccagcaat gcgccatatt
     3721 ccttagcaag tttacgaaca atttggattt tagggtctaa gtctttacgc cagccatgac
     3781 ggtcgtcggt tataggaagt acaaacggct ccatcaagat gatgttgcag atttccgctg
     3841 tttgaagttc atttagtgat tttcgataaa cggattcaaa ttgctgcgca gatttctcag
     3901 ttccgaatat agcctgatcg ctagtattat gccaggtatc attgattccg atcaaaatcg
     3961 atacatagtc aggttgtaaa gctaaacaat cttcttgcca gcgtacttgc agatctgcaa
     4021 ttttgtctcc tcctatccca cggttcatgc attcaaattc catcgttgga tattgggaga
     4081 ttagttttcc ggcaatcaaa agtggataac ctttacctag atcagttgct tctgctcggt
     4141 ctcttcctgc gtctgtaatg ctgtcaccga taaataatat acgttttttc atttgtcaaa
     4201 aactcctttt tgaataaaac gatgattttc ttccttatat tatcccataa gccctatcaa
     4261 gacgaaagag aaaatatatg tataaaaaat gacaccagcc tagtcccttc aatcaattga
     4321 atgaacgctc atcaagtgtc tagtttttta tacttttttt cttaacgtgc cggattcccc
     4381 ttgattcctt cgttgtttgg tccagagaaa tcgatcacac ctaagcttcc gtcaatcaga
     4441 ccactgatga cgccatcaga acatatattt ataggctcat ctaagacttc cagtaaatgt
     4501 tcttttacta atatctcttc tagattgctt gggatagatt ggtcataact ttatctggtc
     4561 cgatgatttc aaggatgaat taaacttaca tatggatcat tgcatgctga aaatacggac
     4621 ctactccatc ttcgaatgtt actattagtt tgttttcagg tactagatgc tgctttattt
     4681 caacagcaga agcatcatca aatcagattt ccatttgaat aaccttcttt gtatatgttt
     4741 gattgattgc gatttcgtta tagaaccttg tgaatcaaat agcaatttct tttgctttct
     4801 tctcttgtct gctggaaact tcctttatcc cgtattttaa aaaaagaact gcgagagttc
     4861 gcagttcttt tttttaaata aacgttgctc tccggccaac tcctcggtca caagtcacaa
     4921 tagtcatcaa acatgaaaaa ctgttttttg aaattactcc ttgtgactcg gagctgaacg
     4981 gcaggcgcac aacctcttga tataaacggt gttcaatcag ctgaccctcg atattagcta
     5041 aaaaaacggt aaaatatgag aagctatttt tccgttttgt ttctctaata ctcaggtcaa
     5101 ggcactgatt tcacaacctc ttccttaaac ggtgttcaaa agccagtttc tcggtattaa
     5161 gccaaattta tgcaaaaatg tggaaagcca tttccacaaa atttttctta atactcgaaa
     5221 ctaaacagct ttttcatcac ctcttatgga taaattcggt tcaacaaacg tgggaatgga
     5281 atagcttcac gtacatggtc gataccagca atccaagtga ctgttcgttc aaggcctaga
     5341 ccaaaaccag agtgcggaac agaaccgtat ttacgtagat caagatacca tgaatattct
     5401 ttttcatcca aaccgtgatt acggatttgt tctagcaaga aatcatagtc tgttgcccgt
     5461 tcgcttccgc caataatttc accgtaacct tctggtgcga tcatatctgc acaaattacc
     5521 acgtcttctc ttgttggatg tggtttcatg tagaatggct tgatggcttt tggatagttt
     5581 aggataaaga caggctgttc gaatgaattc gcaatgaacg tttcatgagg tgaaccaaag
     5641 tcttcgcccc attcgatgtc atcaaatcca tttttcttca gtaattcgat tgcatcatcg
     5701 taagtaatac gagggaatgg caattttgta taagttttta agacttcttt gtctcgtcct
     5761 agtacatcta atgcatattc gcaatgatct aaaacatttt gtactaagaa tgctacatat
     5821 tgttcctgta cttctaaact ttcttcttga tgagtgaaag ccatttctgg ttcgatcatc
     5881 cagaattcga tcaaatgacg tcgtgttttt gatttttcag cacggaaagt cggaccgaat
     5941 gaaaagactt ttccgaaagc catagcagca gcttccatgt ataattggcc actttgagat
     6001 aaataagctt tttgatcaaa gtaatttgtt tcgaatagat ccgtagtgcc ttctggtgca
     6061 gaaccagtca agatcggcgg atcgattttg acaaaatgat gttgattaaa gaattcatac
     6121 gttgcacgaa tgatttcatt tctgatctgc ataatcgcat gttgtcttga agaacgcagc
     6181 cataaatgac gatgatccat caagaaatct gttccgtgtt cttttggtgt gatcggataa
     6241 tcgtggctct cgcttaccac ttccacattt gtaattccga tttcataacc aaattttgaa
     6301 cgactgtctt cacggatttc tccagttact aacacggatg tttcttggtg tagattttta
     6361 gctgtttgga aaacttcttc cgacacttcg ctttttacaa ctacaccttg gaaaaaggct
     6421 gttccgtctc ttaattgtaa aaatgcgatt tttccgcttg atcgcttatt tgcgacccaa
     6481 gcgccgattg ttactgtttc tcccacatgg tttttcgaat cgatgatttg aatttgttcc
     6541 acgtatgact ctccttttat attccttgaa tttcttcagg attaattttg acttttactc
     6601 tcaataaatt gcttgattct gcgtaccgct tcttctaacg tagcgcgatc agtggcataa
     6661 ctcatgcgga cattttctgg tgccccaaag ccttcgccag tcacaagcgc tacccctgtt
     6721 tcttctaaaa gatcttctac ccattgtgtg acattttcat acttacacat gcgtagcgtt
     6781 tcttttacat taggaaaaag atagaaagct ccttgcggct tttcaagaga aacacccggc
     6841 agttctgcca caagcggata taacgtattc aggcgttctt caaaggcgtt ccgcatttct
     6901 tctactgtat cttgctcacc tttcaatgct tcaacagctg catattgact gactgctgtc
     6961 ggattgctag ttgattggga agcaacagcg atcattccat tgatgatcac aggatttccc
     7021 accgcataac caatgcgcca gcctgtcatc gcatatgttt tagacacgcc gttgatgatg
     7081 atggtctgat ttttgattgc ttctgaaatc gttgcaattg gagtaaactc attgccatta
     7141 taaactaaac ggccataaat atcatcagca actatcaaaa tatcattttt tactgcccat
     7201 tcaccgattg cctgcagttc attcttgcta taaatcattc cagtcggatt cgatggagaa
     7261 ttgatgatta cggcttttgt tttttctgaa cgtgcttgtt ctagctgttc aacagtaact
     7321 ttccagctat tggattcttc ccctttcaca aagacaggaa gtccttctgc cagttttact
     7381 tgctcaccgt aactgaccca gtaaggcaca ggaatgatga cttcatcttg cgggtcaagg
     7441 atcgtttgga aaagggtata caaagcaaat ttcgctccgt ctgttacgac tgtttgtgaa
     7501 ggttcataga caagtccata gtttttcttc agataatcaa cgattgcctg tcgaagctct
     7561 gggattcctg ctgttggtgt ataatagcta gctttcccat tgcggattgc ttcgattgct
     7621 gcttcttgga tgttttctgg tgtcgcaaaa tccggttctc ccacagtcaa actcagcaca
     7681 tctctgcctt ttgcttttaa tgccttggcc tttgccgcag ctgccaaagt gacagacggt
     7741 tctaatcgtt ttgttctttt tgataactcc atcccattca ttcctttacc ttttaaattc
     7801 ccttgattgt cttgacttca tctccagatt catagccaat caggtaataa ttcagtttcc
     7861 catttgtttc ttttcctact acttcccaca caggctgatc atcatacatc cctaaagcag
     7921 ctttttcgat ttggatttca ggatgggccg cagcaattgt ctgttttgct tcattttcag
     7981 tcagtccctt tgtttgatcc aatactttta ctttttcacc agatttagga atgataacgg
     8041 cgatttcttt tcctttatca tttgtacctg tcagtgaaaa gtatgtcttc tttcgagtaa
     8101 accaataaaa atgatcggct tcctcaagat ttgcatattt ctttgcgatt tctactgctt
     8161 ctttcttcgc ctgcaccgcc ggacgactgg aacgcaagta aaaaatcgag ctgatcacga
     8221 tgaccgctaa taaaaatacg atcaagccaa gtaacacgcg attaatcgtt ttttcctttg
     8281 tctgtttttt cttcaagggt cgtcgctcct ttatcttcat tccttttcat tatagcagat
     8341 tttaattatg aaaaaagaca gctcctctaa cttccttctt catttttctt taagaattca
     8401 tggatatcct gatagatttc ctccatagct agctctttga tcggtacttt tttcggtaat
     8461 gcatggatga tccgtgcacc atattttgca gtaactagtc ttcgatccaa aataagcaat
     8521 acgccacgat ctgtctccgt ccttatcaag cgtcctaatg cttggcgtaa cttcaaagtt
     8581 gctttaggta cggattcctg gtaaaaagga ttgatccctt ctgattctaa ataagcgttc
     8641 ctagctttga ccatcgggcg aagcggatta tcgaagggta aacgtgtgac tacaacgatc
     8701 tgcaaagcat ctccaggcaa atctacacct tcccaaaaac tgtcagcgcc aaacaatacg
     8761 cttttttctg acaaaatgaa gcgtttcaaa agcttctccc ggcttccgcc aaacccttga
     8821 gctagtattt ctcgtccttt ttctagcata gacagatgca ttttctgata gaccttctgc
     8881 aaaacatcat gagaagtgaa taacacaagt attggccgat cttcttgttc gatcagacct
     8941 gtaagtgctg tctctaaata atcgatatat gcttgattgc tgacttgatg gacggcaggt
     9001 gcctcttttg gcacatagat ccttgcctgt ttctcataat cataaggaga ggcaatcact
     9061 ttaagaggtg ctgcttcaat gccaagcctc ttagagagat agtgtcgatt agggccgatc
     9121 ttcagcgttc ctccaatata aagaatacga tcatatcgag gataccaaac agtcgacggc
     9181 aacatagccg cttgaaaatc atggagatga aaaatcatct gctgcgctgt gttcccagga
     9241 acgaaccaat ggatcaaatg atctcgccag tcttccagcc attgatccat cagcaagtac
     9301 tgcttgtcta attgatcaaa taggctatat aaacttgcta aatcgatccg catctggttt
     9361 gtccagcttt cttggataat ctcaaaagtc agacgcattt gtgactgtag ctgctggatc
     9421 tcctgataaa aaagctgcag gtgatcaatg gctgtcccag attccagtgg cagctgatcc
     9481 aacatctctt ttgtcatcat gacttcttct ggatactgtc cgttgggttg cttgccgctc
     9541 actaaactca ttaatccttc tgccatacaa atctgtgctt cgcatagcgc agaaagttct
     9601 tctcgataaa tcgataacca gcgtaaggct tcatgttctt tcggtaaata gctttgtatt
     9661 cggtcaaaca gctgatttgg ttcttctaat tgctggattt gtttatggaa atagttaata
     9721 cttaatcgtt tgtcatttac tttttcagcg atttccggca aatgatgtac ttcatcgatc
     9781 aacaaaaatg gactagctgg aagcaatggt tcttttcgct gtgtttcttg tactaaaaac
     9841 gcatgattga caatcaaaaa attactttgc ctgattttct tttgcaagaa acgaacaaag
     9901 tcttgttcgt aaaatgactg atttcttgct aaaaatccaa tacctctgtg cgcgatatct
     9961 gcaaagaatg aatggttcaa atttgttaaa tgtaattcat ccaaatcacc ggtcttcgtt
    10021 tgcgtcagcc aaactaagac cgccatttga tacaaagcat attgtttttg ttctggcggt
    10081 tccacaagtg ttcctttaaa tcgttgcagg tcgatatagt ggcgatagct tttgataatt
    10141 acaggttgta tgggctgatc tagcaattga ttcagcaatg gaatgtcttt ttctatgatc
    10201 tgctgctgca aaactagcga aacggtactg atgacagcgg gtttttcagg tgtagccaaa
    10261 taactcaatg gtagcagata accgattgtt ttacccattc ctgtagctgc ttcgatcatc
    10321 aaatcttttg attcatcttt tgtgaaatgg tcgtagaccg cattcatcag tctgttctgt
    10381 tcttttcgat acatcagtgt tttaccaaac attttttctt ttgcttgttt ttttcttgga
    10441 tatgtccgct cgccataatg gacggatgtg aatagttcca cttctttttt ctgtaaagct
    10501 agcccatcga cgatttctaa cgagggatca agtggctctg gtttttcttt catttcttca
    10561 atcacatgat aaatgaaacg actcgtatcc atacctgtgt gtctacttaa tcgcgcaatc
    10621 tgctctaacg tgatgattgg caattttctc atccgttctt cgatcagcaa aagaagttga
    10681 cctgtcactt cggcatcact atctgcctga tgaggattct catgaataaa tccaaggctt
    10741 tctgacaaat cagctaagcg aaagcttggt tccgtcggca agaaaatttg tgcaagttct
    10801 actgtatcga tgcctggtat cttcaaacta ggaagtccgc atcgcatgag ttcatggttc
    10861 aaaaaattat aatcaaaata aatattatgc gcgacaaata tggtgtctgc caaaagattg
    10921 taaatcgttt gggcgacatc ttcgaaataa ggcgcttttt ggacgcgttg attcgtgatc
    10981 cctgttaaat gctggatttg ttttgaaatc ttccgccctg gatgaatatc gatcgaaaaa
    11041 cgtgaagtga tccgacctga ttcaatcatg acgcagccaa attggatgat ccgatcttct
    11101 tttggattcg tcccagtcgt ttcgatatca acgactgcat aagttcttgg tttcaaaata
    11161 tcttcctctt ctcctgcctc taatgttctt ttgaaattat actacaaagt gcagaaaaat
    11221 actctcatcg ctttttaaag aaaatagaca aaaacgccca gacacgcaaa tgtctgggcg
    11281 tttatttttt agtataggtt aattaagctt tagtaacgtt tgttgcttga ggtccgcgtt
    11341 ggccttcttc aacatcgaaa gttactgctt ggccttcttc taaagttttg aatccgtcgc
    11401 cttggatagc tgagaaatga acgaatacat cgtttccgtt ttctgcagtg atgaatccaa
    11461 atcctttttc tgagttaaac cattttactg tacctgtttc cataataaaa atcctccttg
    11521 tgtatcaaca catattttgc aattacttga atggtgtata tctggaagat gtttatttga
    11581 aacaagctac cacagattac cgaacaaaaa ctactcctct agtatatcat accgtttaca
    11641 aaaaagctag agtttttcga ctttttttat taaaaaaagt tttctcagcg attacataaa
    11701 gcgaaaaacc gaactttttt taaaataaac gacatattgt tcagttggtg tttttttgct
    11761 ctgatgttct tttttaaact aaaaaaatta ttctttctga ctacttatcg gttctgtttc
    11821 aacggtcgag gctggggaag attctttagt aggtctgatg ttctcatggt atttctgata
    11881 gcctatgaca gttaccagaa gaacaaggca aaccaatacg atcaacgaga ttagtttttc
    11941 ttttttgctc atatctatac ctcataaata acaggatgat gtccatcata tttctgagta
    12001 tgagtaaaag caaaagctgc agctgtagaa tgtaagatat gccagcaact ttcataatca
    12061 caggtcggac agtaccaaaa gtaaccaaat cttggctgca aagaccgttc ttctgtttca
    12121 ggaatcgcag aatcaagatc tgttgtctgt acataaacat ttttgtacag acgtgtgcct
    12181 gtaataggag accgctgtgt ttcttcagca aaaactggct gacttgttaa acttagggat
    12241 agaaacagca aacaccataa cagtcttttt ttcataaaga cctccttatc gataagtttt
    12301 tcccaattaa aatcatatct caagaacgcc cgttattcac attttgttca taaatgatgt
    12361 tttttgtatc ataatcgtca aaaagaggtc gtgaccacgt cacaacctct tttttaaacg
    12421 gtgttccaga agtagcttct tcggaaataa gccgaaattt cacaaaaatt tgagaaataa
    12481 tttttgtcaa tctcttctta tttcttgaag ctgaccactt ctgtcacaac ctcttcctta
    12541 aacggtgttc aaaagcttgc atctgcggta ataagcccac caaacaaaaa atatgaagag
    12601 ctatttttgc ttgctgttct tattacttga tgcagaacag ctttttcaca acctctcata
    12661 cacggtgttt aatcagctga ccttcggtat tagctcaaaa aacggaaaaa tatgtgaaac
    12721 tatttttccg tttgtttctc taatactcag gtcaaaacac tgatttcaca acctctttta
    12781 ataacgaaga atcgctgtca gactcttttc atctggcgca tctttttgat ccaaaataaa
    12841 cactttcccg ccattaatga tcacatcatc tgctaatgca ttcaacactt gacgccgatc
    12901 atattctgtt tcaggatctt caccgaaacc atcagctaag tttgacgtag cgataaataa
    12961 atgtgacact tttccttctt ttgcagctgg aacgatatca actaactgat cgatgaattt
    13021 cttatctaag agtttattgt attcttttga ttcttgagct gcgagttcac ttgacagctt
    13081 cttcgcccct tcttcgatat ctctaaagga tagctgcgca ggagaagcag aaatcgcaac
    13141 gtctgcacaa tagtaaggat ttttggcgat ttttttgaac atcgtctgat tttctggtag
    13201 agcaaataaa taaatcggcc attgttctgg attttcgaaa gaatccttca agtacgtatc
    13261 aactgcttgg taatagttga cccaatcgat ctctacttct tcatcttttg gatttacgcc
    13321 atggaaagtt actccatctc gggctccgcc tcctccatta gaagagaagt tcaaaccgcc
    13381 acctgtcagt tcagatccca atgcagtaac gacatcaacc ggtgcccctt ctggtagaga
    13441 tacttctttt acttgtttgt tgctgacttc atacaatttc atggagtcgc ggttcaatcc
    13501 caataaataa taagaatagt tgaactgccc atttttgata atagccagta aataaggaag
    13561 atcacttaca tagtattgat cgtctaccgg gatactcaaa cgatggatat aggtttggtc
    13621 agcagttata atgaccgcta cactcgtagt tgcactacgc caaaacgaag gatctcctaa
    13681 caaattttca attttttctt ggaacgggga ccagtcatgt tcggaatatt ttttctcaaa
    13741 acgtttcttt gcttctttcg aaaaattttt gaatttgatc tgatcttttt ccacatcttg
    13801 atgggcgaca tgcgtattca gcatgatcgt gacgaatggt cctttagcgc cttctgaaat
    13861 caattgtgac aataattctt tatccttttt tgccataaac gtacccctcc taaaaaaatc
    13921 attcctttgt ttctatctaa agtctaccac aacactattt ttccatacaa taaaacgtat
    13981 ctaaaaaaat aaaacggttt ctacggtttt aaatttagct atttttttaa gttatcttca
    14041 taatttcctc aattatttag ccccagtctt caactattct tcatgaaatg ctgataaact
    14101 aagaaaaaga ataaaagagg tgtatcttat gcatttgatt atgattttca gtttattaag
    14161 tattatcagt ttatttgctt tcagcgcttc ccgcttatta gaggcggaaa cacagcctgt
    14221 tcctataaaa gtacgaaaaa atgaccgcta accttttcat aacgcgcgtt cttatcctaa
    14281 agtttttttc agaaacattg aaactctctt ccttctctga taaaatttta atttatagcg
    14341 aaacaaatta aatacaagga gatcggatat gtccataaaa aaaacacgtc tagtcctatt
    14401 attggctagt ctcattcttt gccttgcttc ttttctacca gctgttgctg tgcaagctga
    14461 agatacgttc aaagtaaatg caaaagcagc ttttgctgtg gatgcgcaaa gcggtaagat
    14521 tctttatgat caagatggag aaaaaaccat ggggatcgct tcaatcacaa aaatcatcgg
    14581 tctttacatc gtccttgatc aagtaaaaga aggaaaacta tcatgggatg ataaagtttc
    14641 gatttcagat tatgcagaaa atttgagtat caccccagat ttatcgaatg ttcctttgca
    14701 taaagaaaac acctacactg tcaaagaatt attcgactct gcaatcatcc agtcagccaa
    14761 tgcttcaatg gtggccttgg cagaaaaagt ctctggcagc gaagctaaat tcactgaacg
    14821 aatgaaagaa caattgaaag attggggaat caaagatgca acgatcgtta atgcatcagg
    14881 attgaacaac tcttatcttg gcgaaaaccg accagaaggc actggagaaa acgacgaaaa
    14941 tcaaatgtct gctcaagatg tggccatcgt tgcccgccat ttgatcttgg atttcccaga
    15001 aatactggat gtatcaagta ctacgaccca aatgtttggc gaaaatacgc aatcaccagt
    15061 ggaaatggtc aactggaact ggatgctccc aggattcgtt aattacaagg aaggcgtgga
    15121 cggattaaaa actggtacga ctgagcttgc aggtgcttgt ttcgttggta cgatcactaa
    15181 aaacgatcaa cggatcatca ctgtcgtatt gaatgcggac ggccatgcgg aaaacccgag
    15241 tgcccgcttc aatgaaacag accgtttgat ggactactgc tatgacaatt ggtcagaaaa
    15301 agagctcgga aaagctaacg caagtattcc tagcctaaaa accattgatg taaaagacgg
    15361 aaaagaatca acagttcctg ttgtgcttaa aagccctgta aaagtatggg tacgaaacga
    15421 tatggatact ggcaaattaa cgatcacgcc aacacttgac aagaagcaag tcactgacaa
    15481 cgagatccaa gcacctatgg ataaaggtac aaaaatcggt accgctacga tcaccttggc
    15541 agacgataaa ttaggttatt tagaagataa caatgcgcca tcgacagaga tcattacgga
    15601 taaaacagtg gaaaaagcga atatgttcgt gattggctgg cgacgcgtcg cagaattttt
    15661 cggcaacttg ttttaattga acaataagaa aagcatcttt ccatcggaaa gatgtttttt
    15721 tgctttttct agtttaataa cagctttttt catctatttt cgttatactg tataagaaaa
    15781 aggagttgaa tttatgaatc taaccagaga tctgatcttt ttagttctct atcttattgg
    15841 ggtattctct atcgtttatt ggcgaaaaca gaagaaggat ccttccttgt tcttttacgg
    15901 cagtacgtgg acatttacgg taatcttaac ttattttgtc gtgttggaaa ttttttattc
    15961 gaccttttat tttttgattc cattgttgtt ctttagtatc tttgcttttt cttatttcac
    16021 agaaaaacga aggctgctga atggtctttt attcaatgtt ttcctcatca gtttcggcat
    16081 ctacttgttt gtcttgctgt atgaaacgca aaatattttc cttggcggac tgatcgcctt
    16141 gatcacgatt ccattgctac ttgtcctttt atttggtatt tatggattga ttgtttttct
    16201 tttttggaac ggggtcacag ttttacgtag agaatcgcat tcattagcta atcttttaac
    16261 gttgatacta gccatttttc ttacactgtt tcttgttttt gactttttct tgttaaaata
    16321 tttgccgcaa tggataaatg ctttgttttt ctgcgttccc ctgatcttga tctatctatt
    16381 tatcgtattc tacaactttt tgactgtctc attcttgtat cagttcaacc ggcctcgata
    16441 caatcaagac ttcatcgttg tgttaggcgc tgggttgatc aatggagaaa cagtttctcc
    16501 tcttctagcc aagcgaatca ataaggcgat tgctttttat cgggctcaaa gccgtgctac
    16561 attaaatccg cccatcttat tgatgtctgg cgggcaagga gcagacgaaa aagtaccaga
    16621 ggccattgcg atgaaacaat atgccatgga acaaggaatc cctgaaagag acattcttgt
    16681 cgaaaccaat tcgacaacca ctttggagaa catgctctat tcaaaggaaa tcatggatca
    16741 gcaaatgaaa gggccatacc gcgtgatctt tagttccaac aattaccaca tttttagagc
    16801 cggtctttat gcgtgtcaag ccaaattgaa agcaaatggt attggtgcaa aaacagcttt
    16861 ctactacttg cctaatgcgt ttttaagaga atacatcgcc atcttgatga tgaacaaaaa
    16921 aagacatatg atcatttgcg gattgctttt cgcaggctcc gcattcttat cgctgattac
    16981 tttacttttc taaaaaaaca aggtgtgaca aaacatatgt cacaccttgt ttttttcttt
    17041 cattctctta actattggca aaaagagtat cccttttttg accgctactt gctgagcaga
    17101 ataaataccg ctgttgcctg aacacataaa gctgatcaag cagatcataa agaagtatac
    17161 tgcagcttgt gaaccgaaca attcgatccc catcacaaag caggcaatcg gtgtatttgt
    17221 cgcaccagaa aatactccga taaagcccaa tcctgcaaga aaaggaacag aaacatggag
    17281 caaaggcgct agtgctgcac caagcgttgc accaatttca aacaatggtg tcacttctcc
    17341 cccttgatat cccgcgccta aagacaaaac tgtgaagaat aacttcccaa taaaatcata
    17401 gacatgtgcc tgtccattaa atgattcttg aagtaacgga agactcaatc cgagataacg
    17461 ttgtgtttgc aaaatcaatg ctgctgccac aactacagct ccacctatcg tatttcgcca
    17521 aacaggatga ggaatccatt gtgcatatcg agcttttaaa aagacgatcg aacggctgaa
    17581 tacccagcca atcaaaccaa aagcaatccc agcaacaaaa actttgaaga acagcaacat
    17641 actccattca ggaatcacac ccatttggta atgtagatga gaaacaccat agctttctgt
    17701 catgaaattt gcaaacagtg cagcaaagaa gctagggaag atcgcttctg ttcggacttt
    17761 tccgattgcc aacacttcta atccaaataa tgttccagct aaaggtgtgc caaatacaga
    17821 actaaaaccc gcactgattc cactgataat caagatttcc cgttctgatt tatccaatcg
    17881 gaacaaacgt gctgtattat ctgctaacgc tccccccatt tggactgctg ttccttcacg
    17941 gccaacagat cccccaaata aatgggtagt tatggttcca aacagtgtaa gtggaataag
    18001 tcgcaacggg attttttctt ctccaccatt tccttgatcg atcactaagt tgtttccacg
    18061 actagagaga ccgccgtgat acacatagag ataagcaaaa agcgcaccac taactggtag
    18121 tagataaagg agccaaggat gtgatagtcg ggtatctgtc acaactgtca agctatttaa
    18181 gaaaaaagca gataagctcc ccatgaatat accgagaatc gctgtgatca ccagccactt
    18241 cagcatatac actaaaatgc gcattggact cggtaattgt ttttccataa aaaattcctc
    18301 ctcaataaaa aaacgcctac taaagaaagg ggctgtcccc agactttagt aggcgtcatt
    18361 agctatcttt cgttagcatt taaaggcgaa caccatcacc tattcacttc cattaaaaga
    18421 tagcatattt ttctgtaaaa aacaataaaa ttatcgttct ttctttacac gttcagcaaa
    18481 ttcatagaaa ggagccaatt tcgttaactg ttgattacat tggtcactat catgacaaat
    18541 ataattcccc tttttcgtat acgtgccatc agaacctgct ttcgttgtag aaagaaaaag
    18601 cgaaacattc gatgttttct gacagattgc acagattccc ttatttatcg ttggtgataa
    18661 agtaccacta atccctttta atttttcatc ttcgtaatag accatgaatt ttttctgcgt
    18721 acctggatca ttccagccga tgtacgtatg ttcttgcaag tcgatttcat tccaagctgg
    18781 ttgtttgagt tttttcactt ttcggaataa tttctcgatt tgtggtttgc taggctgcat
    18841 aaacggctga acgatttgct tcagctcctc caatgctctt tctgcttgcg cttttgtcag
    18901 ttttacgtct aaaactgtcg ttaaaaatgt atcgatttct agcagttcgt gatctaatat
    18961 gtctcgaatc ttttcagctg ctagtgcctg aactgtttgt accgtttttg gatcattgac
    19021 agatgagtac gcatttaata actgttctac ttctcttttt ataaaaaaat attggtatgg
    19081 ttttattgtt tgttccatga ttttaaagtc ctttcttcta accaaagtgc atactttgta
    19141 gaaaaaagtg atcttctgca aagtatgtgt tacctgaaga tcccggactc ttttaaataa
    19201 gatacgtttt gcatcttatc atcaccccct ttaatgctta ttcttctatg tacatgattc
    19261 attcgataaa taaaagtaaa ccgtatcttg aacagatagt caactgcaaa cagaaaaaag
    19321 gactgtgaca aaagtcgtgt caaagtccct tgtttcgaat gaatggtgtt caaaagcttg
    19381 catctgcggt aataagccga aacttcacaa aaatttgaga agtaattttc gtaaattcct
    19441 tcttatttct tgaagctgac cacttctgtc acaacctcaa tatggtctat tattggtttt
    19501 acctgttaaa tcgaggattc tttcccaaag taacgttgcc atctcttacg catacttgca
    19561 gacatgggct gagccgcttc tttgactgcg cagtatttgt ccacgaatgt cacgatatat
    19621 ccttctttgt acttaggcgg tgcgatcgtt gcaccccaca tgtgcttcaa aatgatatct
    19681 cgctcaagat ccgacaaatc ggtgattttt tctgcattct ttacagcaat tcttggatgg
    19741 atatacgcat gtgttccctc gtcaaatttc gtcgtacgcc aatcatagta gaacagatca
    19801 tgcaaaagtc cagcacgcgc tgttgcttta gcattgccac cccatttttt tgcgagcaga
    19861 taactatagt aagaaacact gatggaatgc tccaaacgag tggaatgaac atgctgtgtg
    19921 taattcgcta attttttcac tgcttctgtt tctaaaagat cttgtacata agacatatat
    19981 tcttcgtctt cacgccattt ttctgtcata attatcgatc tgccaccttt ttgaatttca
    20041 ttttcttctg aaattgatct aagtatacca ccagtgttcg gattattcta gtcgctctag
    20101 ataaaatagc taaagcttaa tagttcctta atcataaaga aaaatgataa agaacgatcc
    20161 ctgctgcaac tccgacattc aatgattccg catttccttt cattgggata tatacgttct
    20221 gatcagtcaa agacaagatt tcctgactta ctccctgacc ttcgtttccc atgattatag
    20281 cgaaatcctc tgttttttct actttgtcta atgcatgtgc ttcttcattt aattctgtac
    20341 cgtacaccgt aaaccctttg cctttcatta cagggattac atctgccaaa tctttttgta
    20401 acacaggcaa atgataattg cttccctgca tgcttcgcag cacctttgta ctataaatat
    20461 ctgccgttcc tttacctaat aaaacaccag ccataccaaa cgcatctgcc gtacggatca
    20521 tcgttcccac atttccagga tcctgtacat tatctagtaa aagccagcct cctgaaaaat
    20581 ctggcgtatc ttcagatgtt aacgtcatca ctgctaagat tccttgcggt gtaggtagat
    20641 ctgacatagc ggtcatgacc tcatctgaaa ctagaaaagt agaaatctct ttgtttacag
    20701 ccagccagtc tccccattct ttcatacctc tctgtgtcac aaaaagttct tttatttctg
    20761 catcagacga cgcagcttct tcgatcagat ggaacccttc caaaagatat tctgcctttt
    20821 cttctctgta tttcttttta tgtagttttt ttaattcttt gattcttgga ttttttgctg
    20881 attggatctc tttcacattc ctcacctcaa ctgattatat catggaatag gtgcttgagc
    20941 ttgtttcatc ctaaaaaaat cggtatcatt gatttataaa acaggaaaga aaggtgacga
    21001 acatgcgcaa agttaaaatg aatgtccaag gacgtgtcca gggcgtggga tttcgctata
    21061 tgaccaagat ggttgctgat cagttaggcg tgaccggaac ggtaaaaaac gaagacgacg
    21121 gctcagtcac aatcgaagca atcggaaatg acgacatcat ccaaaaattt atcgaagaag
    21181 tcaagaagtc tccgagccca agtggacgag tgcagtatgt ggatgtccaa gaagatccga
    21241 tgattgagga acgaaagaaa tttgatgtgg tgggataagg ttgtttttga agcgctttgc
    21301 ctcgagtatt aaggagaaac attttcaaaa tagcttctca tattttgaaa tgtaatgact
    21361 taataccgag aggcagctga taaaacaccg tcaataaggt tgcgaaaaag ctgatctgca
    21421 tcaagcatta aggactggtt gcgaaaaagt cgcttagttt tgagtattaa ggaaaatttt
    21481 gtgaaaatgg ctttccacat ttttgcataa atttgtctta ataccaagaa actgactttt
    21541 gaacaccgtc gataaggttg tttttgaggc gctttgcctc aagtattaaa gacaggtagt
    21601 aaaaaagctg atctatcact tgcagtgagt tcatttgact cctctatttc gggcagaata
    21661 aaaaagtttt tagtttttta aataagaaag aaacacttgg aatcactctt ttctttccaa
    21721 gtatttcttt cttgattttt tgtctttttt caaaattttt cgctacgaaa aagaaagaat
    21781 atttatatga aatttctatg aagttgattg aattccatgc gcttttttag tatagttaag
    21841 tttgtgtaaa aattatagaa gaggaagtat tctatgaata aatggaaaaa ctggttattg
    21901 ggatccggac ttgtcagtat cctgctcttt ttatctggct gtgtgcggat ggattcaaac
    21961 ggaaaccctg acacttcagg aatcgtttat cgagtactcg tccatcctat gggccaagcg
    22021 attacatatc tagttgagaa tttcaattgg tcttacggct gggcagttat tgcaatgact
    22081 gtgatcgttc gtatcatcat cttgccacta ggtatcagtc aatcgaaaaa aacaatgatc
    22141 caatctgaaa aaatgcaagc gttaaaacca caagttgaag cagcacaaca aaagctaaaa
    22201 gctgcaacaa cacgtgaaga acaaatggca gcacaagcag aaatgcaaca agtatatcgc
    22261 gaaaacggat tgagtatgac cggcggaatc ggctgtctgc ctctattgat ccaaatgccg
    22321 atcttctctg ccttgtattt tacggctcgc tacacagaag gtatccgtga atcatctttt
    22381 tacggcatcg atctaggctc accaagtatg gtgcttgttg ccattgcagg ggtcgcttat
    22441 ctgcttcaag gatatatttc aacaatcggt attcctgaag aacagaaaaa gacgatgcgt
    22501 acgatgctga tcgtctctcc tgctatgatc gtcttcatgt cgatcagtgc gccagccggt
    22561 gttacacttt actgggtagt cggtggtgtg ttcagctgtc tgcaaacatt catcacaaac
    22621 gttatgatga aaccacgttt gaaagcacaa gtggcagaag agttgaaaca aaacccgcca
    22681 aaacaagtcg tcactccaag aaaagacgtg acagaatcag caaagccagc tgacaacaaa
    22741 caagcaacaa aacaaatcaa acaaccaaaa aacagtacaa acaacaaagg ccgcaacgca
    22801 ggaaagcagc agaagcggaa gtagatagta aataagagtg taataaaaat tcattaacag
    22861 ttttagcaat taaaaatatc gaccaagatt tttgaaaaag aaatctggtc gatattttta
    22921 tttaagtgaa tgtttaattt gaatcatcga ttgatcgcta tctaattgca gcagcatctt
    22981 tttatgaaga aacaactttt tcttatctat caacgtagac aggtgttgct gtttctttta
    23041 ataatgtact taattcctta atattttgtt ttccttcttc taacatccaa ctactcgctt
    23101 ttttttcgcc ttcggtaaca aaaggtgcca ctcccttcgc ccatgttgct cgcccacata
    23161 agataccatt gaatgtcgaa cctgattgtt ttgcaaaacg taatgtatct tgaaatagcg
    23221 tagcattgac tcccgcactt aaaaaaataa acggttgttc agtcactttc gattgttcat
    23281 agaaatacgc ggatgcctct gcttgactat aacaagtctc ttgcccatat ccttccacaa
    23341 atctcatatt cactggcact tctactttca atacatccac ttgataacgt tcttttgaaa
    23401 attctcgcat ggcttcaata actttatggg gttttacttt tgcataggca agagaagacg
    23461 gatcgccaat agttgtatca taagaaagga tctctaaaaa gaaaggtatg ccttctgctt
    23521 cgcattctga accaatccgt tctataacta tttgtttttg cacattaatc tctgctggtt
    23581 catccacgtc atagtacaat aaaaatttac aagcatccgc accagcttct tttaaccttt
    23641 tcaccgacca aatattcaat aaatcaggta atcttcccgg cattttcgta tcatagccgg
    23701 ttttttcata agctagcagc aagccacatt ctttctctcg aagcgctgct gcaggcaggc
    23761 cgtattctgg atcaagtaaa atagctgagg catagggagt caggtattga gaaaccatct
    23821 gtttaaattg gatgatgtct tctgatgtag cttctttata ttggttgatc attttcttca
    23881 aagcaccacg ctggtcgatt gctaacgcgc taataattcc agcttcatcc gtcaatcgct
    23941 tcaaacgttc ctgcttttct atactcatct tcttcatata tcttcaccta tttctttagt
    24001 taatattgtg gctatttttg tttttgattt tgcttttctc aactgttctg taacctcttt
    24061 tcgcatcaat gatcgtgcaa cgagagacaa taatttcaaa tgagtcgttc ctgcttctga
    24121 atctggaata aataacgcaa aaataaaata gtaaatatct actcgtgaaa acgggtggtt
    24181 tttggcagcc ctagaaggcc tcatcacttg cttatcctct taagaggggc tttttaaggg
    24241 accaccaact tgtaccgacc cccaaaagtt agaccaaaaa atctaacttt tggaggtcgg
    24301 tattttttat ggctaagtat acttttgaac ttaaattaaa gatcgttcat gattatttgg
    24361 atggcaaagg cggaagtgat tatc
//
LOCUS       DAHHZJ010000025        24358 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.026, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000025 DAHHZJ010000000
VERSION     DAHHZJ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.026"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(442..642)
                     /locus_tag="NBL02_000826"
     CDS             complement(442..642)
                     /locus_tag="NBL02_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCD2571531.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(947..2179)
                     /locus_tag="NBL02_000827"
     CDS             complement(947..2179)
                     /locus_tag="NBL02_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCD2571532.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2433..3002
                     /locus_tag="NBL02_000828"
     CDS             2433..3002
                     /locus_tag="NBL02_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCD2571533.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(3180..3620)
                     /locus_tag="NBL02_000829"
     CDS             complement(3180..3620)
                     /locus_tag="NBL02_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCD2571534.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3778..4542
                     /gene="map"
                     /locus_tag="NBL02_000830"
     CDS             3778..4542
                     /gene="map"
                     /locus_tag="NBL02_000830"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCD2571535.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4574..5497
                     /locus_tag="NBL02_000831"
     CDS             4574..5497
                     /locus_tag="NBL02_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HCD2571536.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5573..6712
                     /locus_tag="NBL02_000832"
     CDS             5573..6712
                     /locus_tag="NBL02_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HCD2571537.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6705..7505
                     /locus_tag="NBL02_000833"
     CDS             6705..7505
                     /locus_tag="NBL02_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCD2571538.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7505..8332
                     /locus_tag="NBL02_000834"
     CDS             7505..8332
                     /locus_tag="NBL02_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCD2571539.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            8310..9044
                     /locus_tag="NBL02_000835"
     CDS             8310..9044
                     /locus_tag="NBL02_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCD2571540.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            9144..10010
                     /gene="rfbA"
                     /locus_tag="NBL02_000836"
     CDS             9144..10010
                     /gene="rfbA"
                     /locus_tag="NBL02_000836"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCD2571541.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            10024..10596
                     /gene="rfbC"
                     /locus_tag="NBL02_000837"
     CDS             10024..10596
                     /gene="rfbC"
                     /locus_tag="NBL02_000837"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCD2571542.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10618..11646
                     /gene="rfbB"
                     /locus_tag="NBL02_000838"
     CDS             10618..11646
                     /gene="rfbB"
                     /locus_tag="NBL02_000838"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCD2571543.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11744..12595
                     /gene="rfbD"
                     /locus_tag="NBL02_000839"
     CDS             11744..12595
                     /gene="rfbD"
                     /locus_tag="NBL02_000839"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCD2571544.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12630..14663
                     /locus_tag="NBL02_000840"
     CDS             12630..14663
                     /locus_tag="NBL02_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571545.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14707..15987
                     /locus_tag="NBL02_000841"
     CDS             14707..15987
                     /locus_tag="NBL02_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HCD2571546.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCRSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            16197..17003
                     /locus_tag="NBL02_000842"
     CDS             16197..17003
                     /locus_tag="NBL02_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2571547.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            17015..18235
                     /locus_tag="NBL02_000843"
     CDS             17015..18235
                     /locus_tag="NBL02_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2571548.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            18225..19811
                     /locus_tag="NBL02_000844"
     CDS             18225..19811
                     /locus_tag="NBL02_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCD2571549.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19850..21988
                     /locus_tag="NBL02_000845"
     CDS             19850..21988
                     /locus_tag="NBL02_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCD2571550.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(22356..23339)
                     /locus_tag="NBL02_000846"
     CDS             complement(22356..23339)
                     /locus_tag="NBL02_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HCD2571551.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            23717..>24358
                     /locus_tag="NBL02_000847"
     CDS             23717..>24358
                     /locus_tag="NBL02_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCD2571552.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKL"
BASE COUNT         7690 a         4131 c         4953 g         7584 t
ORIGIN      
        1 cacccttctt cattctagtt ttcgcagcag gaattttgaa atagttctta ttagaatagt
       61 agagattgat taataagcta tcttcgattg ctaaaaagaa aacttctgaa ttccatactt
      121 tataaaaatc tttgataaat ctattcgaag ggtcaaattt aaaccataat tgtgtttttc
      181 cttccatcag cataaaagtt cacctcaaaa agagtatacg aacgaatgtt ctttttgtga
      241 aggcggaatt taatatactg aataatagaa aacaagtact tgtgccaaat tatgtgccaa
      301 aaaaattcga attcaacaaa atgtaaacaa atagaaaacc ttctatatta agatttcctg
      361 caataacaaa cacaacagaa gacgtgtaat agttagtcag gaacgtacaa ataacccctg
      421 tatcctttgc ggtacagggg gttattttgt ataagtagac attgtagttt ctaaactgct
      481 atgtcctaaa cgtttcgata cgctaagtat atttactcct tgataaagta aaatagatgc
      541 atgcgtatgt cttagtccat caataaacag tcgaagagaa aatagggaaa aattgattac
      601 taaagaaaaa cttactaggt cgacggtgta gttgattttc atacttcaat agattcgaat
      661 taaacgattt cgattggctt ataccagtcc tatccaacga aagatatatc gttcttaact
      721 ctagcggtat aacttgaagc tttacgtaaa gccaatggga atgaactgga gatgactttt
      781 tattattttt agtaatagct gatgggttaa tacttacaac tttccttttc aaagtaccat
      841 ataaaaaaac ggtggggttg tttagcgaaa taaaggatct aagccaatag cttagatcct
      901 ttattctatg ctttatatta ttttttgcat gtgtgcgaaa gtatactcaa gcccaatcgc
      961 catttcggaa aatcggtact gtagagccat cttcacggat tccgtcgata tccatcttgt
     1021 cagatcctac catgaaatct acgtgggttt gacttctatt caatccagct tctgctaatt
     1081 cttcatcgga catttctgta ccgcctttca cactgaaggc ataagcagaa cctagtgcta
     1141 agtgattcga tgcgttttca tcaaataatg tattgaaaaa gatgatgcca gattgagaaa
     1201 taggtgaagg gtcaggtaca agtgcgactt ctcctaaacg acgcgcacct tcatctgtat
     1261 caagcagttt tgctaagaca tcttctcctt gttcggcaga aaaatcaaca acttttccat
     1321 ctttaaaagt aaatttcata cccgagatga tggtgccagc ataactcagt ggctttgtac
     1381 ttgaaatgta accatctact cgatgactgt caggtgctgt gaacacttct tccgtcggca
     1441 tatttgccat gaatttttct ccacgagcat tatagcttcc agcgccttcc caaagatggt
     1501 ttttaggcaa tccgataatg atatctgtac cgggagcagt atagtgaagg gcagaaaatt
     1561 gttctcgatt caattcttct gctttttttg ctaatttttc atcatgcttc ttccatgcta
     1621 agacagggtc ttcttcatag acacgagtcg ttttgaaaat ctgatcccat aatgcctcaa
     1681 cttgtttttc ttctggcagt tctgggaaga cttttgctgc ccattgttta ccagccgcag
     1741 ccacgacagt ccaactgact ttatttgctt gagttgcctt gcgtaaattc attagtgctt
     1801 tgccattcgc tgattgataa gacgctactc gatggctatc tacaccagca aatgcatcgg
     1861 gatcggcaga aacaacgctg atcctgcttg ctcctttttc aagccattca tccgcttgat
     1921 cgattttaga ttgtggtaca ttctcaatac gatccgttgc agcatgtaaa agaaattctc
     1981 tttggatctg gtcgtcagtc cattgaacga taacctcagc ggctcctagt ttataagctt
     2041 cttgtgtgat caatcgagca aggggtgcct gttccacgct gatctgtaaa acaactgtgt
     2101 gtcctttttc tgtggctacg ccagtttctg caattaagcg tgcatatttt tttagaagtt
     2161 cattaaaatc agataacata ataaaaattc ctcctcacca ttttttctgg taactattag
     2221 catgatacca tttaattatg attcaaacaa tgaggcttta aataaaaaaa ggaggttatt
     2281 tttttaaggt taagtttcat gatatatgtt actacttgct atttcagctt gtgaagaaaa
     2341 tcaaaaacta attttgcata tcaaaaaaac atgttacaat gaaattaatc aatggtggaa
     2401 gcgttataaa aaaattaagg agattaaaaa atatggcaag aaaaaagacg attactagag
     2461 aacagatttt ggcagctgct tatgaagtag ctgccaccga tggatttaca cgatttactg
     2521 cgagaaacat cgcgaacaaa atgaattgtt ccacgcaacc gatttatctt gagtttaaaa
     2581 atatggatga cttgagacaa gctctttttg agcaaatcta tgatcattta caatatgaag
     2641 tgttcactgt tgagcataca gggaatacga ttatcgatct tgcttttaat tatatccatt
     2701 ttgcaagaag agagaagaag ttgtatcgtt cactgtactt agaagaatat ggtggaggaa
     2761 aagagatgca gaatttctct tatacttact ttgtgaatac agtgaaacgc gatccagctt
     2821 atgtgaatct ttcagaagag cagatcgaat ctttgtataa tgggacatgg atcatagcta
     2881 ctgggatcgc agctttgatg tcttcaagca tcatccatcc aactgatgaa cagattgaaa
     2941 agatgatcca agacagtata gatgctatct tgaaattcaa agaaccaatc gaaatcgatt
     3001 gaatatactc aaaagttcca caaatcagta ggtaacaaga gatgtgtcaa aataaggaaa
     3061 agttcgtttt tcttacagcg tatgcatttt taactcccga agaactcttc catgaatgca
     3121 atgaataaaa gagccataca gattgaataa atctggatgg ctcttttatt gcaagtatat
     3181 taaatttttg cgactagttc tttagcgaaa gcttctaaat tttcgatatc ttcttcttca
     3241 gcattcagat ccactttaac attttcggct ccttttgatg cgcctgtttt tgcaaaagca
     3301 gcatcgaaat catccaccga tttgcagaat tcatcgtaaa aagtatcacc agatccgcag
     3361 acaccgtaaa tttttcccaa tagatccaat tcttgcaagt cttcataaaa gtctacgatc
     3421 tcatcaggca gatcgccatc atcgtatgta taagtagcta ctacgcaaat atctgcctcc
     3481 tcgaattcat ctgcatctac ttgggtacat tcgttgattt cgacttcgat atccagattt
     3541 tcaaatgctt ctgcaacaat atcggcgatt tcttcagtgt ttcctgtcat gctagcatat
     3601 acgatttttg ctaaagccat gatgtgcctc ctcgtttata attatttaac aattatgaat
     3661 tataccaaaa catcattgaa aagaaaataa aacctaaaga cacctgggga atgtcatttg
     3721 gctaaatgta tgccgctatg ttaaaatgag agggatttgt aaaaaggaga cgaaaatatg
     3781 attactttaa aatcagcacg tgaaattgaa atgatggcag aatctggagc tttattagca
     3841 gatgtacata aaaatctgcg tgattttata aaaccaggaa tcacaagttg ggatattgag
     3901 gtatttgtca gaaactatat cgagagtcat ggcggaattg ctgcgcaaat tggctttgaa
     3961 gggtatgaat atgcgacttg tatcagtata aatgatgaaa tttgtcatgg attccctcgt
     4021 aaaaagcctt tgaaaaacgg tgatttggta aaagtcgata tgtgtatcga tttgaaaggc
     4081 ggcatttctg attcttgttg ggcctatgta gttggagaat ctacacctga gatcgaccga
     4141 ttgatggaag taacgaagaa agctttgtac atcggtattg agcaagcaca agtagggaat
     4201 cggattggtg acatcggtca tgctatccaa acttatgtag aaggcgaaaa tctagcgatc
     4261 gttcgtgatt ttattggtca tggcgttggt cctacgattc atgaagaacc agcagttcct
     4321 cattacggag aagctggaaa agggttgcgg ttgaaagaag gcatggttat cacgatcgaa
     4381 cctatggtta ataccggaac ttggaaaatg aaaatggatc ctaacggctg gactgcgtat
     4441 acaagagacg gcggactttc ttgccaatat gaacatacat tggctattac taaagatggt
     4501 ccaagaattt tgacatcaca aggagaagaa ggcacttatt gatctatcca gtcaataggt
     4561 acgggagagg tacatgaaag tttggaacaa aataaaacaa aatgaaaatc tgatgcgctt
     4621 tattgagacg actcaaaagc gcatcactga ttcagaaatg agtacgacct ctgtcgtcgt
     4681 tgcttattat ttactcttgt ctttatttcc attgattatt gcttttggta atattttacc
     4741 gtttctccac atcgatcaag agactgtact gacctattta cgagaagtga ttcctgagac
     4801 cgtttaccag tttatcggtc ctgcaatcaa agaccttttg acacaaagtt caggaggctt
     4861 gttatccgtt tctgcattag cggctttatg gtctgcaagt caaagtatca atgctttgca
     4921 aattgcgatg aataaagcgt atggcgtaga aaatagaaaa aattttttga tcgtccgttt
     4981 cttctcacta attgtcattg tcttgttcat gattgctatt agtggggtaa ctatcgttct
     5041 tgggttggga cagctgattt tggatgctat tcagccaatc gtacaaatac ccgtgaactt
     5101 tatcgatcag ttccaaactt tgaagtggcc aatcacattg gtagctttat ttgtcatcat
     5161 gttcttgatt tatttaattg tccctaatgc tcagttgaag ttgaagacga tcattccggg
     5221 cacgatcttc gctactgtcg gctggatgct gttatcacaa gtgtttagta tctatgctag
     5281 gtattttgca acgaggatca gcggttatca gatcatagga agttttattg tactcatgct
     5341 ttggcttaat tttgctgcta cgatcatcat tttgggcgga atcatcaatg cagttgttca
     5401 agaatacgtg acgggtcatc aaatcaaaaa acgtcgagat ccaagccgaa aatggttcaa
     5461 taaattgaaa actaaatttt ctcataaaaa aaagtagaaa caagagttct tattgtaaat
     5521 ttatatcagt aatttataat aattatgact cttaattaaa gtaggtgccc atatgcttgt
     5581 ttcttttagt atggttataa gattttttct gacaatttta ttatcactga tattaacacc
     5641 tgtattcaaa atcatttcag tacaaacagg tatggtcgac aaaccaaatg aaagaagaat
     5701 aaataaagtt ccaatgcctt cagcgggcgg actgcctatc tttatttcat ttgttatatc
     5761 gactttattc ttattccgta aaattatccc tcaatcgtat attgtaccga tcttgattgc
     5821 ttctttagta atcatagtca caggagtgct cgatgataaa tatgagctga cacctaaaca
     5881 aaaaagtata gggatcttga ttgcttctct tattatttat tttgtagcag atatacgtat
     5941 tgattccttc acgcttcctt ttattggata tatccaacta ggctggctta gttttcccgt
     6001 aacgattttt tggatcttcg gtatcacgaa tgcggtcaat ctgattgacg gtttggatgg
     6061 gctggcggca gggatttcat tgatcggttt aattacgata ggaatcattg gatatttttt
     6121 cctccatgct tctacagtgt atattccagt tgttattttc tgtcttgtag caagtattat
     6181 cggttttttt ccttacaatt tttatccggc aaagatttat ttaggcgata caggagctct
     6241 attcctaggg tttatgatgg ccgtcctttc actacaaggg ctgaagaacg tgacttttgt
     6301 ttcatcgatt tctttgctga tcgtcatggg agtgccggta acagacacgt tttttgctat
     6361 cattaggaga aaagcaaacc gtgtttcttt ttctacagca gataaaaaac atcttcatca
     6421 ccgactaatc agtttaggct ttactcataa aggtgctgtc ctgacgattt atgcgatggc
     6481 tttgatgttt tctttcacag ctatggtcat gaattatact ggaagaatcg gaacgatcat
     6541 attgattatt gccatgttgt ttgctgcaat tttattattt gaattgattg gtctgatcaa
     6601 tgagaaacat cagtttattt taaaaacatt gcgattttta gggaataaag agtaccgtac
     6661 gcaagtaaaa cagaaatatt tgaagaaatg gaaccgtcat atagatgaat aacaaattaa
     6721 accagccgat ccttattagt attgtgacgg caaacagtga taaaattttt cggacactag
     6781 ataattttat ttcaactgta aaagatgaaa cagcagtaaa aatccgaatt tttgacaaca
     6841 attcacttcc cgagtacaaa aaaagactaa cagagtatag gaaatatccc tacatcgata
     6901 tctattttca tgatgaaaat tccggatttg gttatggaca taatcacaat ctgatagcta
     6961 gcgattctcg ctatggcatt atttgtaacc cagatatttt agtggatgaa gagacagtta
     7021 cagcattggt cttgttattg aaagaacatc cggaatgtgc gatgttagca cctaagattc
     7081 ttaatgaaga tggaacaact cagcacctca tccgtgagca gctggctgtt tttgattacg
     7141 ttttgcgctt tgtgcctttc aaatgggtca aaaaaaagtt tgacaaacga ctggctgtgt
     7201 ttgaatgccg aggattgcct actgatgcta atagttatgt acggatgatc tcaggtagtt
     7261 ttatggtagc tgatattgat aaatttaaag agataaaggg gtttgatgag cgtttcttca
     7321 tgtactttga agacaacgac ttgtgtttga gttttgaaaa agcaggttac aagttattgt
     7381 acacaccctt ttatgcagtt atccattttt acgggaaggg tgcgcatcga aatgcaaaac
     7441 tgtttcgcgt gtttttgact tctatgatga aatttttcaa taagtggggg tggcgttttt
     7501 tctgatgaaa ccagaaatat tagtagtcgt tgtgctttat cagcaactat tcagtcaatc
     7561 tccagcttat ggaccattaa gcaaagcact tagcgaaaag caaattcagc ttattattta
     7621 cgataatagt cctcaaaggc agcaaaatga gctgtttgaa aaagaaaata tcgtttatca
     7681 tcatgatccg caaaatcctg gtttagcggt ggcttataat tttgcgttgt ctcaagcaga
     7741 tgaaggaaca cgttgtctcg tcactctgga tcaggatacg aggctagtcg acgattattt
     7801 tgaactattg cgtaaagtaa cctttacaga tgaatgtgtt gctgcagttc cgatgatttt
     7861 ttctgggagc cgacaagttt ctcccctata ttcagatcgt tatatcaatc gtacagctca
     7921 agcagtagaa gttgaaggcc caacagctga acgaatcatg gcaatcaatt caggtgttgc
     7981 ctggtctgtc aaatttctaa aaaagattgg cgggtttaat cctgcttttt ctttagattt
     8041 tttggatcat tggctgtttt ggaaagtgga tcagttggaa aaaactgtgg agatccttcc
     8101 tgtacgaatg gaacatgatc tttcggtttt agactatgaa aaagtttcaa gtaatcgata
     8161 ccgttctatt ttacaagcag aaaatcgatt ttatcatgaa tatgatcaaa ggcatttatc
     8221 cagtcaccga cgacaattat tgttaagaac agctaaacag ttcttgaccg tcaaaaaccg
     8281 gcatatctgg cggatgactt tgcgctcata tgttaagaat tggaaggtgt gaattcgtgc
     8341 gttcggtttg tatagctacc tttaatggag gcagatattt aagagagcag ttagatagta
     8401 ttttatctca gctttcagca gaagatgaag tagtcatttc tgatgatggg tcagtagatg
     8461 atacatggga tatattaact gaatacgcag aaaaagataa gcgaatccag cttttttcgg
     8521 ggcctcgtca aggattgatc gctaattttg gttatgcgat ccagcagacg aaaggagatc
     8581 taatcttttt ggcagaccaa gatgatgttt ggttggagac aaaagtgaaa aagatcactg
     8641 actattttga gcagaatccg gatcagctag tagtagtttc tgatttaatc attgtggaca
     8701 gcgagctgca agagatccat gattcgtatt tttcgtttcg aaattccaaa gaaggatggc
     8761 ttaataatgt gataagaagt cactatattg gtgctggtat ggctttccgg tcttctttaa
     8821 aacccgatat tttgccgatc ccgtcagaag tgccgatgca tgatatgtgg attggtttgc
     8881 tgggaggaaa gaagactggg ttcctaagag aaccgttaac tctgtaccga agacatgatt
     8941 taaacgcgag tgagattgaa acacattcta aattacggca aaaaatttct tggcgtgttc
     9001 atctgattac ggcgttgatc aaacgaaaat ggtttaaacg ttgataaaag atgtttaaat
     9061 ccttaatatt tccatgacaa atataacaat tgatgagata ataacaaagt gaaatttgaa
     9121 atcaaaaagg agagcagata tccatgaagg gaatcattct tgctgggggc agcggaacac
     9181 gtctgtaccc tttaacaaag gcaacatcga aacaattgat gccgatttat gacaaaccaa
     9241 tgatttatta tccaatgtct actctgatgt tggcgggaat caaagaaatt ctgattattt
     9301 caacgccaca agatacacca cgtttcaagg aattatttgg aaacggaaat gatttaggac
     9361 tgcagatcga atatgcagta caagaaagtc cagatggatt ggcacaagcg tttatcattg
     9421 gagaagaatt tattggagat gatagtgtct gcttggtatt aggggacaat atttactatg
     9481 gtggcggtct gtctaaaatg ttgcaacgag cagcttcaaa agatacaggc gcgacagtat
     9541 ttggctatca tgtcaatgat ccagaacgct ttggtgtagt agaatttgat gagaatatgc
     9601 aagctttgtc gattgaagaa aagcctgctc agccaaaatc aaattacgct gtaactggcc
     9661 tttattttta tgacaatgag gtaatttcta tcgctaaagg tatcaaacct tcagaacgtg
     9721 gagaactaga gattacggat gtcaataaag catatttgga taaaggaaaa ctatccgtag
     9781 aacttatggg acgcggattt gcttggcttg atacaggaac acatgaatca ttgcttgaag
     9841 catctacctt tatcgaaaca attgaaaaac gtcagaactt aaaagtggct tgtttggaag
     9901 aaattgctta ccgtatgggc tacatcgata aggaacaatt gctggccctt gcacagccat
     9961 tgaaaaaaaa tcaatacggt caatatttat tgaatttggc cgctgaataa ggagtcctta
    10021 cagatgaaag taacagatac aaaattaaca gatgtcaaaa tcgtagaaat ggacgtcttt
    10081 ggcgatcatc gcggtttctt cacagaaagt tattcaaaag caaaatttgc agaacatgga
    10141 ttgaaatatg actttgttca agacaaccat tcactatcag ctgaagctgg tgtccttcgg
    10201 gggcttcatt tccaaaaagg agaagctgca cagacaaaat tgattcgggt agtcactgga
    10261 gcagttttag atgtagtagt ggatatccgc aaaggtagcc cgacttatgg tcaatgggac
    10321 ggatttatct tatctgaaca taatcatcga cagttgcttg tacctaaagg atatgcccat
    10381 ggattcgtta ctttgacacc aaatgtgaac ttcctttata agtgtgataa ttactataat
    10441 gcagaagcag atgggggcat cgcatttgat gatccagatt tagcgattga ttggccaatt
    10501 gatccatcaa gagcgattat gtcggaaaaa gatcaccgtc atccgacatt aaaagaattt
    10561 gaagcagaaa atccatttgt ttacggagaa atctaagaat taaggagaaa ttttcttatg
    10621 aaaaacatca ttgttaccgg aggtgccgga ttcatcggct ctaatttcgt tcattacgtg
    10681 gtaaacaatc atccagaagt gcatgtcact gttttggata aactgactta tgcaggaaac
    10741 aaagagaact tggccggact tcctagcgat cgcgtagaat tggtcgtagg agatgttgca
    10801 gatgcagaat tagtggaccg tctagtaaaa gaagcggacg cagttgttca ttacgcggca
    10861 gaatcccata acgataattc attaaaagat cctttcccat ttgtacaaac gaatatcatt
    10921 ggtacgtata cacttttaga agcttgccgc aagtatgatg tacgctatca tcacgtatct
    10981 actgacgagg tttacggcga tcttccatta cgtgaagacc tgccaggaca tggggaaggc
    11041 gaaggagaaa aattcactgc ggaaacacca tataatcctt caagtccata ttcttctaca
    11101 aaagctggtt cagatctgtt agtaaaagca tgggtgcgtt cttttggctt acgtgcaacc
    11161 atttcaaact gttcaaacaa ctatggtcct tatcagcata ttgaaaaatt tattcctcgc
    11221 caagtaacga gtatcttaag cggtattcgt ccaaaattat atggcgaagg aaagaatgtt
    11281 cgtgactgga tccatacgaa tgatcattct tcagcagtgt ggctgatttt gacgaaaggc
    11341 agaattggcg aaacctatct gatcggtgca gacggtgaag aagataataa gaccgtgatg
    11401 gaattgatct tagaaatgat gggccaacca aaagacgcat acgaccatgt aaacgaccgc
    11461 gcagggcacg atttgcgtta tgcaatcgat tcgacaaaat tgcgagaaga gctaggttgg
    11521 cagcctgagt ttacgaactt ccgtgatggg ttggctgaca cgatcaaatg gtacgaagaa
    11581 catgaagact ggtggaaaaa agaaaaagag gccgttgaag cagcatatgc caaaaatgga
    11641 caataataca gaggaaattt cactgtatta atggaaaaac acatgtgagg caggcgctcg
    11701 cctgtctctt ttttaaaata aaaatcaagg agcggataac catatgtttt tattaactgg
    11761 aggaaatgga caattaggta cagaattgcg ccatttatta gatgaaaaaa atctgccata
    11821 tgtttcaaca gatgcaaaag acttggatat cacagatgct gagaaaacaa tggcttatat
    11881 tacagaatta aaacccgatg ttattttcca ttgcgcagct tacacagcag tagataaagc
    11941 agaagaagaa gcaaaagaat tagatgagaa aatcaatgtc gatggcacaa gaaatgtagc
    12001 acttgcagca aaagctgtga atgcacaatt cgtctatatc agtacagact atgttttcga
    12061 tggaaaaaac aaaaatgtag aatacgaagt agacgaccaa acgaatccac taaatgaata
    12121 tggtcgtacg aaattattag gcgaaaaagc agtagaagag attttagaag actattatat
    12181 catacgaact tcttgggttt tcgggatcta tggacacaat ttcgtcttca cgatgcaacg
    12241 cctagcggaa acccgcgaca aactgactgt agtaaatgac cagtttgggc gaccaacttg
    12301 gacacgtact ttggcagaat ttatggtata tgtcatcgaa gaaaaagcac catttggtat
    12361 ttatcatttg tcgaacgaaa atagctgttc ttggtatgag ttcgcaaaag aaatattgaa
    12421 agatacagat gtagaagttg cacctgttac gtcagaagaa tttccgcaaa aagcaacacg
    12481 tccgcagtat tcagtcatga gcctgaaaaa gacagaagcg ttaggttttg tcataccaac
    12541 ttggcaagaa gcacttgcgc aaatgctgga agctgccaaa aaaatgaata agtaatttcc
    12601 tttttatcaa atagaatttg gagcatgata tggaaaaaca aacaagaagt gcacgacgaa
    12661 aagaaaagcc aaaagcgaat caggcagatt cgaatttggt gcttgacctg tttctaaaac
    12721 tccgctatct tattggcgtt ttagtcatcg tggtcatcgt tacttttaat ttaaacggaa
    12781 gttcacttgg tgtttgggat aaatatgtgt cgcaaagaga cgatgggaaa aaaactgatg
    12841 tgatttttgg acagaatcgg gaagtgcggt cagacgaatg gctggtgcag acaccttttt
    12901 acctctcaca agctgaagag gacttttcgt tggtgaacga ggattattca ttaaacggac
    12961 aaaacatgat cattgcgtat aattctcctg taaaggatat tacggttatc ggaaaaccat
    13021 tcaactgggg attctttttc ctcggaagag atcgaggact ttccttttat tgggcgatga
    13081 aattagtcgt catggtcttg cttggattcg aagtactgat gatattaacg aagaggaaaa
    13141 aagctctatc gctaatcggt gcatttgcgc tgacattttc tccagcagtc cagtggtggt
    13201 tcatgcaaca tgtgggcgat ttgattttct ttactttggg actgatgatt gctttttatc
    13261 attatttcta caatcatgat aaaaaatggc tacgaacgtt gatgatgctt ttggtggtta
    13321 ttttcggtat tgggtttatt ttagtgattt acccagccca tcaagtgatg ctggcgtact
    13381 tgctgatttt ttattttgtt ggcctgctta tttactatag caagaaagtt tcatgggatc
    13441 tgttggatgt agtgttgatt ggaggggcag ttttatttat tggcggagtc atggttcatt
    13501 tctggctaac gtcaaaagac gcattgttag cttctttgaa taccttatat ccaggaaaac
    13561 gggtaagtac tggaggaaag tggacgattg gcgaattttt ttctttcctt acaaattgga
    13621 aaattccttt tgaagatatc aactattcga ataactctga agtggccctg ttctatcact
    13681 tttttccaac tgtattccta gcaagtccat ttgtgttatt tggcaaaaaa gatagcgaac
    13741 aaaagttact gggacgtatt ttgatgattt tttgtctgtt ttctatcttt tggattaccg
    13801 ttgggttgcc aaaatggcta gcagaaatca ctctgctgtc ttatgttcct ccattaagag
    13861 ccatccttac ttttagtttt gcggcttgtt tattatcgat ctggtttatc gcttatattt
    13921 ggagagaaga tgttattcct atttggtata aattcatttt gttggcagta aatgcatggt
    13981 cttatttcta tgcgattacg cattcgaaaa tggataatta tttttcggat tttgaaacca
    14041 tcgcagttgc tgtgttggga acagtgattt tagcttttgc attatttggc tggagacgct
    14101 tgttttacct cgcttttgtc gccttagtta tcgtatctgg ctttttcgtc aatccagtcg
    14161 tagaaggtac aggagcgatt tttgaaaaaa cactggcaaa agaaatccaa aaaatcgatc
    14221 aacaagatcc tggtaaagtt tggttgtctg aagatgaatt atataacttc accccaacat
    14281 tgggagtgaa atccttcaat acagtacgct tttatccaga tatagctgca tggaagaaaa
    14341 tcgatccgaa agaagaatac gagaagtatt ataatcggta tgctcataca agagctttta
    14401 tcgctaagga agatacaaag ttcattttag atcgtccaga tatgttttcg gtcacgttga
    14461 attttgaaga cgcagaaaaa ttagggatcg actatgtgat cagtaaacga ccattaactg
    14521 attacaacca attatatgaa gcgcagttca cgcagctata tggaccggat aaagacggtt
    14581 atatgatttt taaagtagaa tatccggatt atccagatgt accacaaaca caaactcagg
    14641 aaaatcagct agtcgtacag taataggcta gctgagaata atcctgaaat attagtagaa
    14701 aggagagtga agataattca agcaatcgta caaaaatttg tcaacaaaat gagtgtcggt
    14761 ttcttgcttt tgttgctgac gctagtcttt tttgtttgga cagcaggggt aggttttcca
    14821 gcaactttgt ttatatataa caatagttca ttacttatcg cttttagtgt ggctattttg
    14881 ctcattttaa atgtttataa agcaaaaggc gtcgattttc tttatctagg tgttgcgctt
    14941 gcccttttca ttttcttttc ttactttcgg gaattaagaa atgagtcttt atttggagac
    15001 ttactgtttc ctttgattat tttattttgt ttagtaatta aatggatcga gtggacaaaa
    15061 tgggatcgcg gtatttatct gattgtcttt acggtcttcc tcggaatcac gttatatcgt
    15121 gtatttacag agatccaagt accagaagga caaagcatct gggctccagg caattcgctt
    15181 tctgatattt ggatcaatac gaatacaatc ggaagttctt tgatgactct tgctttattg
    15241 atcagtggat ttgccagttc ttttgaacga tggtatgttc gaatcatcgg tgggatagca
    15301 gtcgttgcag cctttttagg tatttgggtc tgccgatcta gaggtgcatt agtcgcgttg
    15361 attgtttttg cgatattgga tcttttacct aaatcgttga tgcgtgtcat tcgtgcgcca
    15421 tttattgcct acacggttac gattctttta gctttgccga tttcttatct ggcggctgtc
    15481 tctgaaaaag tcaatttgtt taccggtcga gaagatattt ggcataaatt ctatcagacg
    15541 ctaggagaaa aatctgaaca agttttacta ggaatgaaaa catttatctt ccagcgagga
    15601 aaccaatttt taggaaacca caatagttat aattctattt tgaatatcta tggattgata
    15661 ggatttggga ttgctgcttt gctgcttatc ctttttattg gtcgcttaac gttgaaagca
    15721 gatttatcca acgggcagat gacttttatc tgggcctttt ttgcggtgat gatgcagtca
    15781 tttatggagg atacattgac ttcataccca tgggttccga ttatttatat cctgttggct
    15841 atggccgttc atcgatatga tgaaccagaa gttacggagg aattctatgt tatggaagag
    15901 gcatatgacg atatgactta tgatgataca tcttatgagg aacaaccaga tagcgctcat
    15961 tcttccagga tccaacgcta tcattgatag gcacaaaata cgagagaggc tgtgacaacc
    16021 gtcgcagcct tttttggttt attgcaattg tctagcatct tctgtaaaat agaaacgtta
    16081 gagaaaatct tgtatcatag aaaattaaga aagaaataag aacgcagctc ggtaccaaaa
    16141 atatggtaaa atggcacttg actgtaattc actgaaaatc gcttggagga acacaaatgt
    16201 ttaaagattt gtatttattt atgaaggaca tttatcagaa cagaaaatta ctgctacagt
    16261 tttcgatcaa tgacttcaaa tcaagatatg caggatcgtt tttaggaatc ttatgggcgt
    16321 ttatcaatcc gctatttact gtattgattt attggctggt atttggtgtt ggactgaaag
    16381 cacaaatgat cgatgcaaaa tacccattta tcgtatattt agttaccggg atgattccct
    16441 ggttcttctt ctcagatgca tttgcatcaa ctacggttgt ttttcgggaa tatacttatt
    16501 tagtaaagaa agtcgtattc aatattcgta tcttgcccac gacgaaaatc ctatccaatc
    16561 tgtacaccca tttattcttc attctgattg gcatagctgt cgcgcttata aatggtatat
    16621 atccttcagc aatgtcttta cagctgattt attatttatt ttgcatgtgt gctttcttga
    16681 caggattgac ttggctgacg gcatcgatcc aaccattttt acctgatatc atgcaattaa
    16741 ttacgatcgt gctgcagttg atcatgtgga cattaccgat attatggagc cctaatcagt
    16801 ttgattcagg tcttgtaagc atattgaaat taaatccgct gtactatgtt gttcaaggtt
    16861 atcgtgagtc atttttaagt gaagcttggt tctgggagca tggaacatat actttgtatt
    16921 tctgggtttt cacgttagta atgttcgtaa tcggctctgc agtgttccgt agattgaaac
    16981 cacatttttc agatgtgctg taaggagaat agctatgaca gaatatgcaa ttagactaaa
    17041 agatatcaca aaatcttata atatgtacgc aaaaccttcg gatcgatttc gtgaagccct
    17101 caatcctttt aagaaatcct atcatgatgt attttatgcg ttgaaaaacg tgaatgtaga
    17161 aataaaaaaa ggtgagatga tcggtttcgt gggagaaaac ggatcgggaa aatctacaat
    17221 gttgaaaatc attacgggag tactgacacc aacatccggc acgatggaaa tcgaaggaaa
    17281 aatctcagct ttgctagaac taggatcagg attcaatcct gagtattctg gctatgaaaa
    17341 tatctattta aatggaatgg ttctaggatt ttcaagagaa gaagtcgatg aaatggtcga
    17401 tgatatcatt gaatttgctg acatcggcga ccatatttat caacctgtaa aaacctattc
    17461 aagtgggatg ttcgttcgtc tagcttttgc tgtagcgatc aatgtgaatc ctgatatttt
    17521 gattgtggat gaagcattgg cagtaggaga tcttgaattt caactaaaat gtatggaaaa
    17581 atttacagaa atcaagaact cgggaaagac gattttattc gtttctcatg atattagttc
    17641 gattcgccgt ttttgtgatc gttcctattg gttgaaaaat ggaaaagttg tcgaatatgg
    17701 tgatacgatg gaagtaacga cgaattacga aaactttttg aagaaaaaat cagttaaaac
    17761 agtagatcga aataaaaacc aagtagaacg cttcgcagct ccagatatcg tagacgtgac
    17821 gaaagccgaa ttgttggata aagataaaaa tccagtggat atgctggaac aaggagaaaa
    17881 actatacgtt aaagtaacct atgaagtaaa agacgataca atcaaaaatc cagttttagg
    17941 aattgctctg cgtacagtcg acaattttta tgtgtgcgga ctcaatactc tactagatgg
    18001 tatcaagatc ccttggaaaa aaggtgagaa tatcttttat ctggagtatg aaaagttagg
    18061 tttgttagct ggtgaatatt attttgacgt tgcggtcttt gaagaaaatg caacggttcc
    18121 tttggtatac aagacaaaat acatgaactt gtttgtcagc ggttcttata tcggagaagg
    18181 catcgtggtc ttagaccata aatgggaaga agggactcat agcaatgaaa tatgattttg
    18241 aaatggacct agatgaacaa agcagtgtgg gaaaaattgc cgcacagatc aaaccaggaa
    18301 gtaaagtttt agagtttggt cctggaaacg gccgattaac gaaacatttg atcggagcaa
    18361 aacaatgtga agtgagcatt gtcgagctag ataaggaatt gttcgatttt gtcagcgagt
    18421 ttgcccaaga tggattttat ggagatatcg aaagttttga atgggcaaac tattatgctg
    18481 gacaaacatt tgattacgtt ttgtttgccg atgttttaga acatttggta gatcctggaa
    18541 aaacgttgaa gaaagttaga gaatttttga atgaagaagg agaaattcta attacattcc
    18601 ctaaccttgc gcataattct gtcatgattg atttgttcaa taatcaattg ccatgggctt
    18661 cttatggttt gctagatgaa acccataatt ctttttatac tcatgatggc ttccaaaaag
    18721 tttttgaaaa agcaggatta tacatcaata ttgaagatta tttgtactta gctgtaggtg
    18781 atacagaatt aaaatcaact tatgaagaat tgccggaagc tgtacgttat gatttcaaaa
    18841 tgcgaccatt tggcgaagtc taccaatact tcttctcatt gatgaaacat ccagtagcac
    18901 aaagttccat tgcgcagccg caaaattcta attatgtgaa agtattagaa gtaatccagc
    18961 aaacgaaaca agatgaaata attcaacaaa taccattcaa taatttcaca ggggaaaatg
    19021 agacactaag tttccatgta tcagaattag cagaacgaat ggtgtttcgc ttcgcgcagc
    19081 aacctagctt tatcgaattc agcgcagaag ctgctggaga aaaactcaca tttatcgatt
    19141 caaatgctgt agtcaaaaca gtaaatgatt gttatttgtt tgatggtaaa gatttaccag
    19201 agttcttttt aacggatatt gcagggaaag aagtgactat ccattgtcac tatcgtttta
    19261 tcggagaatt gacaccaaca atgaaagaat tgctggaaac aatccgtcca atggctgaag
    19321 ttacaaagca gctttctgag aaaaatgatg aattagaaag agaaaatcat tatcttattg
    19381 agaataatac aagattagat cagacgttga aaacaaccat aaatcgctat tgcacgttgt
    19441 tggaaagtga tgaatggaca atcaaacata gattagggcg acgtaagaaa gaaacatcga
    19501 aaaaaatcca agaaaaagag ctttctctat gtattgacga aaaaatctgg gatgcagaaa
    19561 caaagatatt gaaaatcatc gggtggggaa tcgctaattc ggatcgccaa ccattgtcct
    19621 ataagttatc agccgaccaa tcaccatttt ttgaagcaat tcctgtttta cgagaagaag
    19681 tcaatcaagc aaaacagcta gctgaaggag caaaagcagg atttgaactg cggatccttt
    19741 gcgagaagga acgttcattc ttagttgaag cagtggctca aaatggtcaa tcatggatca
    19801 ttgaaatgta aataatgatt aacttataaa gataaggagt aaacgaatcg tgcaaaaaaa
    19861 agaaattgcc gtatttatcg atcaaatcac tcgtgaacgg gccactggcg atttgcttat
    19921 tgtgggctgg gcaattgatg aagtgaccaa agaaatccct acgataaagg tagaaaaaga
    19981 aaatgtgatt gctgaagcaa cacatgtcgt aagattggat atcaatcatt tatataatct
    20041 tgatgttaag acccaaagcg gttttaaggt acgtttatca gggaaaatga gaggaaaagc
    20101 aatcttagac ttccaaacag caagacatca aaatggcata gctgtgaaat taaacggtaa
    20161 atatccttat gatgacggca ttgaatcttc atgggaaaga aaaaaaagat tactgaaaaa
    20221 agggataaat tatgcaagga ctcatggcgt aaaaaaagca atccgccggg tcaaactgga
    20281 attgaaacca ggatcgatcg actatgcaga gtggatcagt cgtcatgaga aacctgatct
    20341 gaaagagcaa aggaaagaag tacaaggatt tgcctaccgc ccattgattt ccatacttat
    20401 gcctgtctat aacgttgaaa tcaaatggct cgaaaaatgc attgattcgg tacttgatca
    20461 aacgtatgat cactgggaac tatgtatcag tgatgatgct tctactgatc cagctatccg
    20521 aaagtgtcta gaatcctatc aagcaaaaga tgatagaatt aaagtcgttt tccgacaaga
    20581 aaatggacac atcagcctgg caactaattc tgcattggaa atggcagaag gagagtttat
    20641 agcgctctta gataatgatg acgaattacc tccatttgcc ttatatgaag tggcaaaagt
    20701 gctaaacgta catccagaac tggatttgat ctacagtgac gaagataaaa tcgatgcaga
    20761 tgggaatcgc tttgacccgc acttcaaagc agactggtct cccgatacat tgatgggaaa
    20821 taattatatc tctcacttag gcgtatatcg gacaagcatt gttaaggaac ttggcggttt
    20881 ccgtaaagga tatgaaggct ctcaagatta tgatttggtt cttcgtgtga cagaacaaat
    20941 tcctgcagaa cacatttacc acattgaccg tgtcttgtat cattggcgga cgatcccggg
    21001 atcaactgca agcaatggag aagctaaaag ctacatctat gattctggag tgaaagcatt
    21061 gacagatgct ctatctagaa gaaatatcaa gggaagcgta catcctggca gaatttctgg
    21121 tttttatgaa atcacttatg atgttttgca agaagacttg gtcagtgtga tcattccaac
    21181 taagaatggc tatgaagatc taaaaacttg tgtggattcg atcatcgaaa aaacgtctta
    21241 tccaaattat gaaatcatta ttgcagataa tggaagtaca gatcctaaaa tgcaagaatt
    21301 atttgcagaa tacaagcatc agctgaaaga ccgatttatc gtcgaattaa ttgacattcc
    21361 attcaattat tcacgtatca acaatctggc agcagaaaaa gcaaacggca aatacttttt
    21421 attcttgaat aacgatacag aagtgattga acctgactgg atgacagcga tggtttctta
    21481 tgcacagttt gatcgaatcg gctgtgtagg agcaaaactg ttttatccag atgatacaac
    21541 gcagcatgca ggagtattat taggaatcgg tggtgttgca gggcatgcat tgaataacta
    21601 tgatcgaaca cattgtggtt atttcggccg attggtcatt gacgtcaatt acttagcagt
    21661 cactgcagct tgtatgatgg tcaaggcagc tgacttcaat gctgttaacg ggtttgatga
    21721 aacattagaa gtagcattca acgatgttga cttgtgcttg aaagtttatg aactgggtcg
    21781 ttacaatgtc tatgcacatc aagcagaatt gtaccatttc gaatctaaat caagaggtta
    21841 tgaagatact ccagaaaaac aaagacgttt tgctggtgaa atcaaaaaaa tgcaagataa
    21901 atggccagca tatattgcgc atgatccatt ttacaatgat aatttaacaa aagaaggaat
    21961 cggcgacttt tcgttgagac cagattaaac tataataaaa agctgttatg ttgtgattcg
    22021 ctcgcacata acggcttttt atagtttttt gtcaaaaaaa ggcagaaggt gccgactcaa
    22081 aaaaataagt cgaggttggg acagaagtgt ttaactccga gaaataagaa gaaattcacg
    22141 aaaattgctt ttcaaatttt tgtgaatttc ggcttattta agaagaagtt gcttctgctc
    22201 ccaccgttta ttcgttttta aagaatgaga caaaagtgtt tcttactttt gtcccacgct
    22261 cttattcatg ctcagagcgc caatagatac aaaatcatag ccaaagtttc acgaacccta
    22321 gctgtcaaag aatcgatatt ttattttaat ataggctatt ttaaaataaa tggtttaagg
    22381 tattcctcta caaaagccac tctatcaatt atcatgggtt taaaataaga agtgatggag
    22441 ataaccagat tgttcttagc gtcaatataa agaacatttc cactattacc aattgctgaa
    22501 tatatttcgt tctcagaatc taatacccac cataaatagc catatctcat attttgtaag
    22561 tcttctaaag cactggttct aggtttgaac atctcgttta tccacatggg tggtacgatt
    22621 tgttttcctt tatacatccc tttatttagg cataatacac caatttttgc cagatcactt
    22681 gccgataaac acaaaccata tcctgccgtc ccgatattta gtggatcaca aaaccagaca
    22741 ttggttttag gagacttatc cattgtaaac tgcttatgtt cttcagctgt ttcagcataa
    22801 taattgacat ggtttggtat ccctatagga tcaaatagat acttattggc aaaatcaaca
    22861 gttgttagct tacttgtctg atacaacaag ccagttaata tgtgtaaaca gacagtttga
    22921 tagttgaatt cgccggttat accttttctt cctcccaata aatctagtga tgtttttgtc
    22981 caatcttcac ttgaacagac ttttgtccat ggatcgcctt tacatttgta gggtgctttc
    23041 attgttagca agtgcttgat tgtaatagcg taaatagttt tctccccgcg tttcacttta
    23101 tactctggaa aataatctaa tactttatca tcgatacttt tgattaatcc ttgctctaaa
    23161 gcaatgccaa tcagcaaaga tacaatactt ttcgttgctg acatgatatg acaagtatcg
    23221 tcttttttat aaccattcca ttcatctgta taaacttcta ccccatcctt atatgcgaca
    23281 atttgacata tatttggttg tcgaaaacca ataaaatcat gcaactcttg tttattcata
    23341 agatattacc tcactttctt ctataagcat aatgtcatta aaaatcgatt tcaatttttt
    23401 cgattcttca cgtaatacag gtattcttat ggactagaca gcgctttggg aagatggatt
    23461 ctctgaaaaa tataaaggtt cactttatga gtggataaaa aaagcagcaa atacttcgac
    23521 ttctttaatt taccaacatt agtgaaaatc gattcatctg gagatttgta atttagagtc
    23581 cttgtcttga ttagaggtgt ataaagcttt ttattagaca tttttaaatt ccattacaat
    23641 ttgcttataa actaggtttt taaaggtgac tatggtaaga taatagagat attattttgt
    23701 gattaggagt gtatatatga ataaaaatgg agaatggagt gctgcacgac ggatattcat
    23761 tatattaatg gatgttattg tatacaatgc agcaatatat agtagtttct tactgaagtt
    23821 tcgtggagag atcccattac gaaatttaga aactttccaa cactcagcaa ttattatttc
    23881 agtgattttt attgcgttga acattttgtt gggtgcatat gttttttata atcgaatgat
    23941 cagtgacatc gtgtttgtca cagtcatcgg tcaggtattg atgacactag ggatcatggt
    24001 agtgactttt gcgggccgct ggtttgcttt tccacgtctt gttatcttac tttcatttgt
    24061 tctcggtacg cttttcttaa gtatctatcg tattctcgta tataaacttt atttacgcgt
    24121 aagtcgagat aaaaaggtcg tcattattgg gttagaaaag gatgtcgcac cagcaatcca
    24181 aaattttggt tcaaagaaaa acaacaaaca taaagtcgaa gcagtcattc ttgataacta
    24241 ttatgcaaac atcaaaaata tgattaaaga catcgatatt gtctatcttg cttctcatat
    24301 cgaagaaact gaaaaaatca aaatctatca attagttact aaaaatgaaa aaaaattg
//
LOCUS       DAHHZJ010000026        23484 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.027, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000026 DAHHZJ010000000
VERSION     DAHHZJ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.027"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(201..908)
                     /gene="deoD"
                     /locus_tag="NBL02_000848"
     CDS             complement(201..908)
                     /gene="deoD"
                     /locus_tag="NBL02_000848"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCD2571553.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(925..1743)
                     /locus_tag="NBL02_000849"
     CDS             complement(925..1743)
                     /locus_tag="NBL02_000849"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCD2571554.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            complement(1767..2933)
                     /gene="deoB"
                     /locus_tag="NBL02_000850"
     CDS             complement(1767..2933)
                     /gene="deoB"
                     /locus_tag="NBL02_000850"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCD2571555.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            complement(3032..3988)
                     /locus_tag="NBL02_000851"
     CDS             complement(3032..3988)
                     /locus_tag="NBL02_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2571556.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            complement(3988..5121)
                     /locus_tag="NBL02_000852"
     CDS             complement(3988..5121)
                     /locus_tag="NBL02_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2571557.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            complement(5114..6676)
                     /locus_tag="NBL02_000853"
     CDS             complement(5114..6676)
                     /locus_tag="NBL02_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2571558.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            complement(6888..7970)
                     /locus_tag="NBL02_000854"
     CDS             complement(6888..7970)
                     /locus_tag="NBL02_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HCD2571559.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            complement(8036..8434)
                     /locus_tag="NBL02_000855"
     CDS             complement(8036..8434)
                     /locus_tag="NBL02_000855"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCD2571560.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            complement(8455..9117)
                     /gene="deoC"
                     /locus_tag="NBL02_000856"
     CDS             complement(8455..9117)
                     /gene="deoC"
                     /locus_tag="NBL02_000856"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCD2571561.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            complement(9163..10464)
                     /locus_tag="NBL02_000857"
     CDS             complement(9163..10464)
                     /locus_tag="NBL02_000857"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HCD2571562.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            complement(10605..11621)
                     /locus_tag="NBL02_000858"
     CDS             complement(10605..11621)
                     /locus_tag="NBL02_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCD2571563.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            complement(11929..12534)
                     /locus_tag="NBL02_000859"
     CDS             complement(11929..12534)
                     /locus_tag="NBL02_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCD2571564.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            12730..13653
                     /locus_tag="NBL02_000860"
     CDS             12730..13653
                     /locus_tag="NBL02_000860"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCD2571565.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            13712..15277
                     /gene="guaA"
                     /locus_tag="NBL02_000861"
     CDS             13712..15277
                     /gene="guaA"
                     /locus_tag="NBL02_000861"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCD2571566.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(15768..17399)
                     /locus_tag="NBL02_000862"
     CDS             complement(15768..17399)
                     /locus_tag="NBL02_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HCD2571567.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(17413..19086)
                     /locus_tag="NBL02_000863"
     CDS             complement(17413..19086)
                     /locus_tag="NBL02_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HCD2571568.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(19099..19416)
                     /locus_tag="NBL02_000864"
     CDS             complement(19099..19416)
                     /locus_tag="NBL02_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCD2571569.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(19441..20841)
                     /gene="lacG"
                     /locus_tag="NBL02_000865"
     CDS             complement(19441..20841)
                     /gene="lacG"
                     /locus_tag="NBL02_000865"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HCD2571570.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            21078..21863
                     /locus_tag="NBL02_000866"
     CDS             21078..21863
                     /locus_tag="NBL02_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCD2571571.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            21866..22558
                     /locus_tag="NBL02_000867"
     CDS             21866..22558
                     /locus_tag="NBL02_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCD2571572.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(22832..23203)
                     /locus_tag="NBL02_000868"
     CDS             complement(22832..23203)
                     /locus_tag="NBL02_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HCD2571573.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
BASE COUNT         6775 a         5239 c         4231 g         7239 t
ORIGIN      
        1 tattcatcaa acatgaaaag ctgttttttg aaattgttcc ttgtgactca gagttaaacg
       61 gcagattcac aacctcttgg taaacggtgt tcaaaagctt acatttgcaa taataagtcc
      121 agccaagtca aaaacatgag gagctatttt tgcttggatg ttcttattac ttaatgcagg
      181 gcagcttttt catttccttc ttattctgct atcgctgttt ccaatcctac ttcgatcatt
      241 tcattgaacg tagattgacg ttctgcagct gttgtttctt caccggtgat caaatggtcg
      301 ctaacggtca tcaaacttaa tgtctgcaca tggtatttcg cgcctaagta ataaagagcc
      361 gctgcttcca tttccactcc taaaacgcca agttcagcta attggaacgt ctctgtcaaa
      421 tcatctttgt agaaagaatc ttccgaaaga acatttccta catgaaccgt atagcccttt
      481 tctttagcta tctcatatgc tttcagtaat agattgaaat cactgattgg aggaaaatga
      541 aaggcttgga aatttttttc gatcatcgaa gaactggtcg ctgcaccttg tgctagtact
      601 aaatcacgaa catggacgtc ttttgacaat gcaccgcatg tacctactcg aatcaatttc
      661 tttacaccat acgactggat caattcatgg gcatagatgg tggctgatgg cattcccatt
      721 cctgttcctt gaacagaaat acgttctcct ttatatttgc cggtaaagcc taacataccg
      781 cgtacctgat tgtaacaaac tggatcttcc aaaaaagtct ccgcaatata tttagctcgc
      841 aaaggatctc ccggaagtaa aatcttatcg gcgatctcgc cttctttcgc ttcaatatgc
      901 acactcattt tattgttcct cctattacaa taatgtcaat gtttctttaa ccaatgcttt
      961 aaaagattgt ttgactctct gagttgtttc tacaacttct tcatggctca gattcgcttg
     1021 catacctgct gctaagttgg taatacaaga gattcctaac acttttaatc cgctgtgagc
     1081 tgctacgatg acttctggaa cagtcgacat ccctactgca tcggctccta ttgtccgaga
     1141 cattcggatc tccgcaggcg tttcataagt tggtccagaa tagcccatat atacgccttc
     1201 tttaagatcc aaacctagtt tttctgctgc tgcttttgct gttgcttgat agtcaggcgt
     1261 ataagcttga ctcatatctg gaaatctagg gcccattgca tcttcattcg gaccgatcaa
     1321 aggattttca ccagtaaaat tgatatgatc ggtaatcagc atcaaatctc ctggtacaaa
     1381 tgattcgttg acaccaccac atgcattagt cacaatgact gaatgggccc ctagtccggc
     1441 catcacacgt actggatagg tcactgtttg catggaatga ccttcatagt agtggaatct
     1501 cccttgcatg gctaacactt tcttgcctgc cagagaccca taaactagct ttcctgcatg
     1561 cccaactact gtcgaaacag gaaagaacgg gatctcatca taagaaatca ttacagcatc
     1621 ttcgatttca tctgctaatt cacctaatcc tgaacctaga atcaaaccaa aatcaatttc
     1681 ttttactcct ttatcaagga taaactgggt cgtttcttgt aacatctcgc ttaacttggt
     1741 catttgcttt ctcctattta aaaagattat tttaataatt tcaagaagct ttcgccattt
     1801 tcagtcgaag aaacactaaa gttctcagca atcgttgctg aaatatctga ataatatcct
     1861 tgaggcaatg ccccttgttc tttcattttt ttactgtaaa ccaataacgg tacatattct
     1921 cgcgtatgat cggtaccagt aaacgtcgga tcgtttccgt gatcggctgt aatcattagc
     1981 aagtcgtctt cttccatcgc ttcaagcaat tctggaattc gtaagtcaaa ttcttcaatc
     2041 gcatgcgcat agccgactac atcccgacga tgaccgtata atgcatcaaa atctactaaa
     2101 ttggtaaagc tgattcctga aaaatcgttt ttcataactt tcagcagctg gtcaacgcca
     2161 tccatattgc ttttcgtgcg gacagattca gtgattcctt gtccgttgaa aatatcgttg
     2221 atctttccaa cagcaatcac gtcctttcct gcttctttca aagaatcaag gactgtgtgt
     2281 ccgaatggat tcaacgcata atcatggcga ttgcttgttc gtgtgaagtc tcctggttca
     2341 cccacgtacg gacgtgcaat gattcggcca atcatatacg gatcatcttt tgttatatca
     2401 cgaacgaatt ggcaaatccg atataattct tctaaaggaa tgatttcttc atgtgccgcg
     2461 atctgaagga caggatcggc agaagtatat acaattaaat cgcctgtttt catttggtgt
     2521 tcaccgtaat cgtcaataac agctgttccg ctataaggtt tgttacatac gactttacgg
     2581 ccagaaaatt cttcgatttg tttgatcaga tcatcaggga atccattagg aaacacacga
     2641 aatggagttt tgatattcaa tcccatgatt tcccagtggc ctgtcatcgt atctttccct
     2701 acagaaactt cttctaattt cgtcgcatag ccttgatgat ctttcactga ttccacacct
     2761 tcaagaggac ggatcgttcc taaaccaagc tgctctaaat gtggaacgtt caatcctgct
     2821 tctttcgcaa tgtgtcctaa tgtatcagaa ccaacatcac cgaacttatc ggcatctggt
     2881 gcttctccaa ttcctactga atccatgacg atcagatgta cgcgtttaaa catgattatt
     2941 ccttctttct ttttattttt attgccttta aaacaaaaag ggcgagacga aaaacacttc
     3001 ccgtaaccca ccctttttat tcgctaaaag attattttga tttgatatag ttcttcccgt
     3061 ttgcttttgg accagaagct ttccctaaga aaacaaccag cacaatgatt gtcagcaaat
     3121 atggagcaat ttgcagcaat actggcggaa tcgaagaaat aactggtaat tgtgcgccaa
     3181 tgatactcaa gctttgagca aaaccaaaga acaatgaggc acccattgcc cctaaaggat
     3241 tccacttacc aaagatcata gcagccatgg agatgaaccc ttgtccaacg atcgtaccag
     3301 ctgaaaagtt accggaaatc gactgagcaa aaacagcacc gcccataccg cctaacaagc
     3361 cagaaatcag tacgcctgaa taacgcatag catatacatt gatacctaat gtgtcggcag
     3421 cttgaggatt ttcaccaact gaacggaggc gcaagccgaa tttcgtcttg aagatgatca
     3481 cccaagctag gacagcaatc aaaatagcac aataagcagg taagaatgta tttttaaaga
     3541 aaattggtcc aatgaccgga atatctgcta atcctgggaa agaaaaatac cccagattct
     3601 gcgtgatgtt atcagtttga cctttaccat accatgcttt gatcaagaaa acacctaaag
     3661 caggtgccat cagattaatt actgtcccgc tgataatatg gtcagcacgc aaactgactg
     3721 ttgctactgc gtgaatcaga gaaaatagga tacctacgac gccacccact aaaatagcga
     3781 tccatggtgt catagaacca agtgatggac tcatcgtcaa gttgaaaacg atggaactaa
     3841 aagcacccat gaccatgatt ccctctagcc ctacgtttac gatcccgctt cgttcagaaa
     3901 aagttccgcc tagtgacgtt agaattagcg gtgttgaata aaccagtgta gaagtaagga
     3961 tcaaggcaat taattcgata gtcgacatta tagttcgccc ccttcatggt tttgattcgt
     4021 tggtttcgat tctattgtcg caacgatctc ttcttgtttt ttcccttgga agactttagc
     4081 catgacgaaa cgaatcagga agttgatccc gacaaagaag ataatcaaag caatcgatac
     4141 attgacgatc tcaaacggaa cacctgctcc tgtctgcatg ccaagtccac cgatcttcaa
     4201 aatactgaac aagatcgctg ataaaagaat accgattgct gatccagaac caagcaagga
     4261 aacagccatt ccgtcaaatc caatagatag cgaagtcgtt tgaacaaaga agttttgata
     4321 cgttcctaat ccttcaacca caccgccaag ccctgccaaa gcgccagaaa tgaccatcga
     4381 caaaacgatc gtacgtttac tactcattcc tgcatactca gaagcaaaag gattcaatcc
     4441 aacagaacgg atttcaaatc ctaaagtcgt tttcttcatc agcagccaaa cgaaaataag
     4501 cgcgatgatt gctaaaaata atcctgagtt tacgcgagag ccgccaaaag cttgtgttaa
     4561 ccatggtaac cgcagtgagg cattttgact gatcatattg gaagagtcca aacttgagcg
     4621 atagctttca ggcatcactt catgtaaagc aaatgtgctt acgtataata agatatagtt
     4681 catcatgatt gttacgatca cttcacttgt tccaaaaaat gctcgaagta aacctggaac
     4741 agccgctgcc atcgcaccag ctaaggcacc taagatcacg cataacggaa gcaaaacaac
     4801 ttttggcaga tctggaaacg caagtgccac ccagacactg acgatccacc cgcaaagtgc
     4861 ttgtccagaa agcccaatat tgaaaaaccc agctgagtta gctacagaaa aacctaatgc
     4921 tgtaaaaatc aacggcgttg cttgccttaa cgtttcacct atccctcttt gcgaaccaag
     4981 ggctgcatct agcatagaag aataaccttg gatgggctga aaaccaaaag cagccatcaa
     5041 tatcgcgcct aagatcaatc ctaaaacaac tgaaagtaca ggcactaata aattacggaa
     5101 tttttcgctg cgatcattca tgtgcgccag cccccttttc taactctttt cgtgcttctt
     5161 ctaaggtata gcccgccatc aatagaccta attcattttc agatgtttct ttcggatcaa
     5221 cgataccgac aatttctcct tcatggataa cagcaatacg atccgaaaca tttaaaattt
     5281 cttctaattc gaaactgatc agtaacactg ctttgtattt gtccctttgt tcgatcagac
     5341 gtttatgaat aaattcgatc gcacctacat ccagtccacg agttggattt gcaacaatca
     5401 acagatcagg atctcggtcg acttcacgag cgataattgc tttttgctga tttccgcctg
     5461 ataaagcttt tgcgggaaca agttcattcg ttgttcgaac atcatattct tcgatcaatt
     5521 ctctggcatg ttcattcatt actgaataat tcagcatgcc gtttttactg taaggttttt
     5581 gatgataggt ttgtagtgcg atattttcag ataatgtcat atctaggact aacccgtatt
     5641 tatgacggtc ttctggcacg tgtcctacac catgttcagt gatttttcgg ggttttttat
     5701 tagtgatgtc ttcccctttt agcttgatat gtccgctttc tgcctttcgc aaaccagtca
     5761 aagcttggat caactccgac tgcccgtttc catcgattcc agcgatacca agtacttcgc
     5821 cagcacgaac ctctaagttc aagtttttca cggcttctaa tccacgattt tctttcacca
     5881 ctagattttc aatagacaaa acgacttctt gaggcttcgc ttcttttttc atcgttttga
     5941 atgaaaccgt tcttccaacc atcatatcgg ctaattgttg tgaggttacg tctttaacgt
     6001 tgactgtacc gattcctttt ccacggcgga taactgtaca gcggtcagct actgctttga
     6061 tttcatctaa cttatgcgta atcaaaatga tcgacttgcc ttctttgact aattccttca
     6121 tgatcacgat taattcatca atttcttgag gggtcaatac agctgtcggc tcatcaaaaa
     6181 tcagtacatc tgctcctcgg taaagtgttt ttaaaatttc tacccgttgt tccattccaa
     6241 ctgaaatatc acgaacgtaa gcatctgggt tgactgacaa tccatactgt tcagaaactt
     6301 tttggatctc ttttcgtgct tttttatggt caagcatccc tgcgcgacta ggctcgcttc
     6361 ccaacacgat gttttctgtt acagtaaagg catcaacaag cataaagtgc tgatggacca
     6421 tcccaatccc taattggttg gctttggtcg gacttgtgat ctttacttct ttcccacgca
     6481 tcaaaatttt ccctgatgtc ggctctaata atccggaaag cacgttcatc aatgtagatt
     6541 tgccagcacc gttttctcca agcaacgcat gaatttctcc tgcctttact tgtaaattga
     6601 tgttgtcatt tgccttgaat gttccaaatt ctttggtaat attctgcatt tctatgacaa
     6661 tattttcttg aaccaccttt ttttcacatc ctaacgattt ctatctaggg caacgattga
     6721 ctaagattca cgcttagtga gcgttcttct tttgaaacaa acattacttc agggagttgt
     6781 tcccttatcc aaactgtcac aaggcagctt ggataaatga acaattcttg ttctaatcaa
     6841 accatagcct tgtcaaacat gtgtctaaca aggctataga aatggtttta gttttcttca
     6901 ggagtttctg gaacttttac gtcacctgaa atgatttttt cttttgcttc atcaactgct
     6961 tttttcgctt cgtcagaaag ttgtccttct gttagaccaa caccgtcttc tttcaatcca
     7021 taaacagtgt gttcgccgcc agggaattta ccgtccgctg atttttgtgc caaatcttct
     7081 actactgttc caactgcttt cagtgtagat gtcaatgtga agtttttctt ttcgccgttt
     7141 aatgtgtatt cgccttcgtc tgattggtcg cggtcaacac cgataaccca aacttttttg
     7201 tcgcctgatt cgttcaatga ttttgcttct tggaatacac cgttacctgt accgcctgaa
     7261 gcatggaaga tgatatctgc gttttgtgca tacatacctt gagcaattga acgtccttta
     7321 tctggagcag agaagtcacc agcatattga ttcaatactt tgatttcttt tcctaattct
     7381 ttcgcacctg catcaacacc ggctttgaat ccagcatcaa aacgatcgat tacttcacct
     7441 tttacaccgc cgataaagcc gactacgttt gtttcagtcg tataagctgc tgcaacacct
     7501 gcaagataag aagcttcgtt gtctttgaaa gttgcagata caacgttatc taatccatcg
     7561 atgacatcat cgataataac aaagttcgta cctgtattgt ttgttgcttg ttcttggatc
     7621 gctggtttta gtttataacc aataccaaaa atcgttttga atccagcatt caatgcttgg
     7681 tcaatatttg ggatgtaatc tgattcattt gaagattgga aatattggaa gccatcattt
     7741 cctcgtgaaa gtccttgatc tttcccccat ttttctagtc cttcccaagc tgactggttg
     7801 aatgaacggt cgtctacacc acctgtgtct gtgatcaatg ctgcagttgt tgtgtctgat
     7861 gagcttttgc caccggatga atcagtagag ccgttgttgc cgcatgcacc taaaaataac
     7921 cctgctgcta gtgcgacagc acctaaacca aataattttg cttttttcat ttctgtaaag
     7981 cctcctaaaa taattgtttt atttttcaac agtcagctga ctgaatgaac atgagttata
     8041 gatctttttc agtaaatgca taaggcagca gtgcacgaac ggtcgttgcc ttcgtcacac
     8101 cattgtctcc tactagcgta accggcatat caggggaaca gaattctgcc attacttgtc
     8161 gacatgcacc acaaggagag ataggatcgg gggtgtgccc cgctacgact agatgcgtaa
     8221 atgttttttc tccttcagat actgctttga aaaaagctgt gcgctcggca caattcgtta
     8281 atccaaatga tgcattctca atgttgatgc cttgataaat cttgcctgat tccgttacga
     8341 gacaagcgcc taccggaaaa tgggaataag gaacataggc tttatctaac gcttcaatag
     8401 ctgtgttgat ccattcttgt ttgagttctg ccatgagctt ctcctttgaa aaaattaata
     8461 gccttcacca gttgaaccag atacgattgc tactcctgcg cttgttccga ttcttgtagc
     8521 accagcttcg atcattgcca ttgcttcttc ttcgttatgg ataccgccgg aagctttgac
     8581 acccatttct ggtcctacag tttcacgcat caaacggaca tcctctacct ttgctccgcc
     8641 tgttgagaaa ccagttgatg ttttgacaaa atcagcgcct gcagcttttg ctatttcgca
     8701 agctcggatg atttcttctc tatctaataa cgatgtttca ataataactt tgacaagcgc
     8761 acgatctttt gctgcttcaa caactgcttc gatatctttt tggacttgtt gatcatcctt
     8821 tgatttcaaa gcaccgatat tgatgaccat atcgacttca tctgcaccgt tattgatggc
     8881 atctgttgtt tcaaatgctt ttgtctcggg tgtgtttgct cctagaggaa acccgattac
     8941 tgtacatacg gctactggct caccttgtaa ttgttctttc gctagcgcaa cccatgctgg
     9001 attgatgcag acagaataaa aatggtattt ttttgcttct tcgataattt gcatcacagc
     9061 ttctttagat gcgtctgctt tcaaaatagt atggtcgatc attcggttta gttccatttc
     9121 ttttcccctt ttctttaaaa cggtaaagtc cgcttttttc ctttattctg tgatgatgtc
     9181 atgaatcaac gttggcttca ttgccgattc accaatttca atatttttat ataataattc
     9241 aatgacagaa gtgatttctt cgtcgttact atagatcgtc aatagtgact caccttttgt
     9301 cacagtgtca ccaatttttt tatgcagttt caatccaaca gcgtgatcaa tatcttcttc
     9361 tttggtttta cgcccagcac ctagcatcat tgcagcaatt cctaattcat ttgccacgat
     9421 ttttgacaca actccgcttt gtttagcagg aagctccatt gtgtagcgtg cagtcaaaat
     9481 tcgttctgga tgttccacga tcgctgggtc tcccccttga ttttggatca tttctttgaa
     9541 ttttgctaac gctttacctg attccagagc ttcttgaagc aacgttcttg cttcatcggt
     9601 tgtcttagct ttgccagcaa gaacaaccat ttggcttcct aaggcgtagc acatttctac
     9661 tagatcttct ggaccatttc cttgtaatgt ttcgattgcc tcgacaactt ctagactatt
     9721 tccgatagct tcgccaagag gttctgacat atctgagata accgccactg tttctcggtt
     9781 agccagtttc ccaatgctcg tcatggtctt tgccagacgt cttgcatctt cgatgttctt
     9841 catgaaagca ccgtctccag tggtcacatc caaaacgatt gcatctgctc ctgccgcgat
     9901 ttttttactc ataatcgaac tggcaatcag cggaagagaa tcaacggtcg ctgtgacgtc
     9961 acgcaaggca tacaattttt tgtccgccgg tgccaagttt cctgattgac caattaccgc
    10021 tactttactt tcattaacaa tgcgtacaaa ttcttcatcc gaaagctcga tctgaaatcc
    10081 gggaattgct tctagcttgt ctagtgtacc acctgtgtaa cctagaccgc gtccagacat
    10141 tttagccact gtcactccga cactagctac taggggggct aaaatcaatg tggtcgtgtc
    10201 accaacgcca cctgtcgaat gcttatctac ctttattcct tcaagagaac tcaaatcgat
    10261 gacatcacca gaattagcaa tcgccaatgt taagttcgtg atttcttcat ttgtcatgtc
    10321 ttgataaaaa attgccatca ataatgcact gacttgataa tctggaatct ttccattcgt
    10381 atagttcgtg acgatgtact cgatttcctc tttcgacagc tcgtttccat ccctcttttt
    10441 ttcaatcaga tccaccattc gcattgcctg atcctccttg tcttcattcg gtattataca
    10501 ttatttttag gaaaggtaaa ccactttagt aaactggttt actaaaaata aaaaaagcac
    10561 taactagcct ttgtaaatgc ttacataagc aatataccgc cgagttagcc ctttgtcaat
    10621 acttttacgc tctcacgaat aattaatttt gtttcgaaca ctttgttcgg aacttttctt
    10681 tgaggaaatt cgatcgtatc gatcaggaaa cgtgcagcat aaaatccaat atcaaaactt
    10741 ggctgctgca ctgtcgtcaa ttgcggaatc atgtattctg acagctttaa tccatcgaat
    10801 cctatgactg agatatcttc tgggattttt ttgcccaatt catcgatcgc ctgatagcat
    10861 ccgatcgcca tggcatcatt cccgcaaaaa atagccgtga tatctttatg tttgagtaat
    10921 ttttttgatg cagtatatcc accttggaca gtcaactctc catgttcaac aaatttccca
    10981 ttaaaaggca ggccatgatc tttcagcgca tgacgatatc cgttgaaacg ttcttctaat
    11041 tgatagtatc cagtggtttc cttcagcatg ccgatcttcg tatggccatt ttcaataaac
    11101 agcgaaacag cttgataggc gccctcatat tcctttacga taagccgtcc gttatcccga
    11161 cgattcaatc cgcgatcgat taaaatgatt ggcatttttt gataaaaacc gctgtctaat
    11221 gcatatttct caggtaaaat atttggtgtg gccagcaaga ttccgtccac tgagcggtgg
    11281 gaaagctctt gtaaatactt gatctcattt tcttggctat gacgagaatt gcataacatg
    11341 atcacatagc ccaaaggatt caaatagctc tctacacctt cgatcagttt ggagaagaag
    11401 aaatcagtga catcagggac gatcattccg atggtctttg aatgtcgatt gatcacattt
    11461 gaagcgaaat aatcgggctt ataatgatgt tcattcacga tcgacaacac tttttttctt
    11521 gtcttatcac taaatctgct tcctttgtta ttcaaaatct gtgagacagt tgttactgat
    11581 acgcctgcca tttctgcgat atctctgatg gtcagcttca tcctattggc tcccttctat
    11641 ctaaattccc tatcagtgta ccaaaacccc caagaaaaga atagagaaaa aaggaaagta
    11701 tgttttttga taaaaacctc ctttatctac cgacttggca gccttctata caagttctgt
    11761 atcttattta ttttatcgta aaaaacaacg actctgttcc tactatttct ttttgaagaa
    11821 aaaatagttc tttgttttta aaaacttctc aacaaaaaaa gaactgatac gtacccgtac
    11881 atgcacgccc agttccactc tttcgtcgct ccactatttt tcagctagtt attctttcac
    11941 acttttgatg atataatatc ctttatcttt tgtgacgatt tctgcattac caaatgtttc
    12001 ttccattttc ttttgcgcac tcggcgctcc ttgtttcttc tggatcacaa ttgtcagtgt
    12061 accgcccttc ttcaaccgag gatatgcgcc tgtcaaaatt tcatgtacaa ctttttttcc
    12121 agcacggatc ggcggattgc ttacgatagc tgcataggtt tcttcatgaa ctgcttcata
    12181 gatattcgat tgatggatat caacatgttc gatttggtta cgctttgcgt ttccttgcgc
    12241 tagacttacc gctcgttcat taatgtcgac catttcaaca aaccgctgtg tcgcaaatgc
    12301 caaagctaac ccaatcggtc catacccgca tcccacatct aagatctttc cttcatcagg
    12361 taactcttcc caatcgaaag catcaatcag gacacgtgaa ccataatcca ctgtttcacg
    12421 tgaaaataca ccagaatctg ttacaaattg aaaggttctg ccttttaact caaaagccca
    12481 ttgttcaaaa tcgtgcgccg tatctgggcg ttcagaatag taatgatttg tcatgtcttc
    12541 catccttctc atttctttca tgacaacagt ctatcctttt atgtatccgt gcgcaagtca
    12601 agaataagac tccgtttctt tctattataa agattataca aaaagatcgg caatctctct
    12661 taagtggtgg ggtttgcgtt tattttgtgt taaaatagtt ccattgatgg gagaacgcag
    12721 gaggctaata tgaatgatcc aatgaactac taccgtgtgg caagagaaga atggcgagaa
    12781 ttttatcgta atgggcaagc acctttgaca caagatgaac tagataatat caaaagtttg
    12841 aatgaccgca tttccatgca ggatgttcga gatatctatg tacctcttgc tcatctgatt
    12901 tatctttata tgaaagaatt tgaatctttg ttcttgagta aaggtttatt tcttcataaa
    12961 tacgtagaag taccgccttt tattatcggg atcgccggaa gtgtagccgt tggaaaaagt
    13021 acaacagcca gactgctgca gacgattttg tctcgtacct tcaaacgaag aaacgttcag
    13081 ctgatcacga ctgacggatt tctttatcca aatcgtatat taaaagagcg tggaatcatg
    13141 gaccgtaaag gatttcctga aagttatgat atggagatgc tgatcgattt cttgaaccgt
    13201 gtaaaatctg gtcaggaaga gataaaagta cctgtttact cacatgatgt gtatgatatc
    13261 attccagaca agtacgaatt gatccagcag cctgatatct tgatcgttga ggggatcaat
    13321 acgcttcaat tacctgcgaa tcagcaaatc tacgtcagcg acttttttga tttttctgtc
    13381 tatgtcgatg ccgatcccgt gctgatcgaa aaatggtatt tggatcgttt tggtgcgtta
    13441 ctggatacgg ctttccaaaa tccagagaat tattattatc gttttgctat cggagatcgt
    13501 gaagaagcct ttaaaatggc aaaagatgta tggaaaaatg tgaatttgaa aaatttacag
    13561 gaatatatct tgcctacacg aggaagagcc gatataattc tccataaaac tgaaaatcat
    13621 atcatcgatt cgattttcat gcgaaaatat tagcatttta aaatagtttg actattttga
    13681 gataaaattt aaagggatag gggtaataaa tttgacaaac gttgccgata tgacgaacgt
    13741 tgaaaagatt atcgtattag actacggtag tcagtataac caattgatca caagacgtat
    13801 tcgtgatctt ggagtctttt ccgaattatt gagccaccgc attactgcag aagaagtaaa
    13861 agcgatgaat ccaaagggaa tcattctttc tggcggacca aacagtgtat atgatgagaa
    13921 tgcatttggg atcgatcctg agatcttcaa tttaggtatt cctgtattag ggatttgcta
    13981 tggtatgcaa ttgatcacgt acaaacttgg cggttccgta gagcctgcta aaaaccgcga
    14041 atatgggaaa gccttgttag aagtaacaag cgatcaagct gtcttgttta aagaaacacc
    14101 aaaacaacaa acagtatgga tgagccatgg tgacttggta actaaagttc ctgaaggctt
    14161 tgaaacagtc ggaacaagca aagattgtcc gattgcttct atccaagaca caacacgtaa
    14221 tttttatggt atccaattcc atgcagaagt acgtcattcc gaatacggaa tcgaattgct
    14281 gcgccacttt gcctttgatg tttgtcactg tgaaggtaac tggacaatgg ataactttat
    14341 cgatatgcaa gtagaaaaaa tccgtgaaca agttggtgac aaaaaagtct tgcttggtct
    14401 ttcaggtggc gttgattcta gtgtcgttgg ggttctttta caaaaagcga tcggtgacca
    14461 attgacatct atctttgttg accacggtct gctacgcaaa ggcgaagcgg aacaagtaat
    14521 ggaaagtcta ggcggaaaat tcggcttgaa catcatcaaa gtcaatgcaa gagatcgctt
    14581 cttagacaag ttgaaaggtg tatctgaccc agaacaaaaa cgtaaaatca ttgggaatga
    14641 attcgtatat gtttttgatg acgaagcaac aaaacttgct ggtgaagaag gcgtctcttt
    14701 cctagcacaa ggaacgttgt atactgacgt tatcgaatct ggtacagaaa cagcacagac
    14761 gatcaaatct caccataatg taggtggact gccagaagat atgcaatttg aattgatcga
    14821 accattaaac acattattca aagacgaagt ccgtgaatta gggactcagt taggcatgcc
    14881 tgattcaatc gtttggcgtc agccattccc tggtcctggt cttggtattc gtgttcttgg
    14941 cgaaatcact gaagacaagc tagaaatcgt acgcgaatct gatgctatct tgcgtgaaga
    15001 aatcgcagct gctggacttg accgtgatat ctggcaatat ttcactgttc ttccagggat
    15061 ccgctctgtc ggtgttatgg gtgacggacg tacttacgac tacacagtcg gtatccgtgc
    15121 tgtaacttca atcgacggaa tgaccgctga ctttgcccgt atcccatggg aagtcttgca
    15181 aaaaatctct gtacgtatcg taaacgaagt agcccacgtc aaccgtatcg tgtatgacat
    15241 cacttctaaa ccaccagcaa cagtggagtg ggaataaaga gccaacataa tatggagggg
    15301 gaatccctat aaatcaacat tcttcaatag ctaaaatgct tgattttcat taaaaataag
    15361 atggttccct accaaaagag actataatat aagttaattt gtttcattct tatcataaaa
    15421 agaagttctc gaaaaacgaa ctgttttccg agaacttttt tctatccatc taagtgctca
    15481 aatcatattc acgacggaat tctaaggtta ccctttctta aacaaacttt atgttatctg
    15541 cacaactttt tgcaattttt tcattgcttc ttacgaaatc gttgaactct ctttagcata
    15601 atacaaagtg tagatgaagt ctggtgcttt actcgttttg gtaaaaatga ctggaaatcg
    15661 tttctggtta tttttggcaa aggcgaggtg caggttcgat tcctgccttc tacgctaacg
    15721 atgtggctga agggaacctc cgatcttgaa tcgaaacgat ccgcagtcta ttgtttcacc
    15781 aatgaaacaa aatcaacgtt cgtccaagca tttcctgaag ccttgctata aaccccaatt
    15841 tcacaaacac ctgttgtaat tggaatgtct ttgattgtaa tttttgtcca ttcacttgtt
    15901 ttaacaaccg cagtatttag ttccgctcca ccataatttt tgatatataa ctgattagta
    15961 tcaaactctc ccccagacat tacataggca ctcaatgtgt aagttccttt gcttatgcca
    16021 ctaacatttt gataagctga tgcctcgtat gcttgatcct gccaaaaagt tagtttgtag
    16081 tccccaaaaa aagtgcccgc ttccgttttg atcgtaccct tagcggttcc ttgttgaaac
    16141 caggtttgcc aattggaagg actggtcgtt ggtccatcgc tttcgaaact tgcattttta
    16201 actagattta tttgcgcccc tgcttgttta aacgcacgca agcttggcaa ggcgtttcca
    16261 gcaaaatcaa acatcgcttg gttgtcccat tcgtttccca tagtggattg aacactgaga
    16321 taattcatac ctgcttgtgt tgcccaactc acatttccgt tgtaccaaag aggttcccaa
    16381 tagaagaagc ccgccccgcg attattcggt actgcctgaa tccgatcggc aagatccagc
    16441 aaatactctg cttgtcctgt cggtgttggt tcatatcccc ccgcagctgc ttcggcttca
    16501 ccaaaagcat ttggcatagt atcggcattc gctgttgtat gcccataagc tgtttcaact
    16561 acgatcactt ctttaccata ccgttggctg atcgcattca tattggtttg aagttcattc
    16621 attgaaccgt gccaatacgg ataatacgac actccaatta aatcaaatga cacacctcga
    16681 ctcgtgattt catcaaacca ccaagtaaat gctcctgtat cgccaccatc agctaagtgg
    16741 agcattactg gagttgtttt aggttgagtg tctcgaacag cttggatacc agacttcaaa
    16801 aaagcagcca atcgatcaaa ttctcccccg ccttctcccc aagacttgcc ataaggccag
    16861 agcattccgc tattcagttc attgccgatc tgaaccatat caggataaac gtccagctgc
    16921 ttcatttccg acagaacgtt ctttgtatag tcgtacaatg ccgtattcat ttcttcaaag
    16981 gtcagacctt gccatgcttt aggaagattc tgtttcccag gatcaaccca aaaatcactg
    17041 tagtgaaaat ccagcagcac cttaagtccc agattttttg ctcgttttgc aaggtcaatc
    17101 gtcgtttcca gatcatttgt ccctgctcca taactttctc cattactatt ataaggatcc
    17161 tgccacaaac gcagtcgaac atagttgaca ccattatttt ttaaaatctc taaggcatct
    17221 tttgaaatat cgtcttcata atattttgca ccagattttt ccatatctga caggatcgaa
    17281 atatctgctc cgcgaataaa ttcgctggca gatgcttcaa cagtttttga aacagccccc
    17341 aaagccccta acacagctgc aaataaaaca gctttacgca accaaattcc cattttcatt
    17401 atggctcctc ctttattgaa attggttcag aataaactga acagcttttt caggatctct
    17461 cgttaatccg atatattctg ctccttttgt cgcaaccagc ttgattccta agcgatcggt
    17521 gtcttctttg atatcatcat agtaagaatt gacttgcggt gctaagacca ccatatcaaa
    17581 atcctgcagc atttcgtaat gagaaccata agcacccgcc attgcactga tcggtgcttt
    17641 cgattcagat gcaccttttt ttaaagcatt agctaacatc gcacttgtcc ctgcgccagc
    17701 acaaagtacc aacacattga tttctttatt tgcatcgctg ataacttgac tcgctgccac
    17761 aggtacttcc tcacgtacat cggcagaagc accttcctcg gttgcgatca cctctgcttt
    17821 ttgtgcttct ttctctacta agacagcatc gtatgctttg caaaatggta gatagatcag
    17881 gaagtcaacg acgattagaa ccgctgctag aacaaaagct aagataccca tgcccgttcc
    17941 tagaaccaaa ccgatcggtg ctggagttgc ccaaggcaaa acgtacataa agctgttcat
    18001 gcccaataca tcgacaaaaa acttgaacag gcaaacgttt acaacaggtg ttaacaaaaa
    18061 tggaataaaa aaataagggt tcaaaatcat gggtgccgca aatagcaatg gttcgttgac
    18121 agcgaaactt actggaataa aggaagcttt cccaactgcc ttcaactgct tagacttagc
    18181 aaagagtagg aagataaaag gaacaaccaa tgttgcccca gttcctccca tagttcctac
    18241 aaaattccct aaacccaccg tcagtacttt agaagcttgt tcaccggaat tgaacaattg
    18301 cagattggtt tccacattgg catagataat tgccgcaatt gccggctcca cgatcgacgg
    18361 tccatgaaca ccgacaaacc aaaacagcgc catcgctccc cagataatag cgattcctaa
    18421 gtacccgtca gctgctgtaa atagcggctg cagcaccgta atgattgctt ctgcaaatga
    18481 gatttggaag atttgtcgaa tgcctaaatc aattaaaacg gccgcaaata ctgaaaaaga
    18541 gaaaggaaaa atatctcgga aggtttgcga aatcgttcca ggaacttctt taggcatttt
    18601 aatggtaata tctttcagca cacaaaactt atataaatta actgttaaaa atgcagcgac
    18661 aaaggaagcc agtagtccct ttgtccccat atattccgtt gaaaatccac cttcaatcgg
    18721 agagacgctt aacaacagga aacttaccac agaggccagc atcacagagg tggtgttgat
    18781 tactttgttt ttcggtaagc gacgattcat cgaatcagcc aagcagcgag cagtggttgc
    18841 tgaaacgagt aaccctacaa cacccatcga ataattatag actttccata gccatgtgga
    18901 gacagattca ggccattggt aaccaaaaat ctcaggaacc gcagcaatca aaatgaaaat
    18961 gcttgaaaat aaaatagctg gcatcgctgt taaaaatccg tcacgaacgg ctcctaaata
    19021 actattacgt gagatttttt caaaaaatgg tttccctttt tcgatttgtt taatgattcc
    19081 atccatagcg ttcttctcct atccttgatt ctgtctgtac agtacaataa agtcttgaat
    19141 cagatctctc agcaataaag tggtcattaa atgatcctgc ccatggataa aaatgaatcc
    19201 catctccatc tctttcccag atgcttcttc cgctaaaatt tttgtttgcg aattatgtgc
    19261 aagggttaaa ttttcgtcgg cttctttcaa cgccgaatca acatgttcaa agtttccttg
    19321 tcttacttct ttcaataatt tcaataatgt actgcgagcg tcgcctgaat atgcaacgat
    19381 ctcaaaacca agcatttgta attcctcttt attcatcttt tcttcctcca ttatcactta
    19441 ttaaataatc gttttggttt cactgaccaa cttgtaccag taagctgatt cttttgggta
    19501 acgcttctgg gtttcaaaat caacataaaa caagccatac cttttgttat acccatttgt
    19561 ccatgagaaa agatccataa gcgaccacaa aaaatacccc ttcacattta ccccggctgt
    19621 tatcgccttg cttaaggatt ccaaatagac tctcaaatag tcgattctcg gctgatccat
    19681 aataatgcca tcttcaaact gatctttata ccccatcccg ttctcagtga tatagatttt
    19741 gttgtaatgt gggtaatcgc ttttaattcg cagcaacaag tcatatagac cttctggata
    19801 aatcagccag tcccaatcag ttcgaggaat tccttcttta taaatacgtt ctccgattcc
    19861 cttcacttta taaacagagg tccctttttc acccgtaccg ttatgatgga ttgcattttc
    19921 tccatcgtaa gctttgacaa aatggcattg gtagtgattg atccctaagt agtcattacg
    19981 cgtggatgct tttttcaatt caacgaaatc ttcttcagga aaatgatagc tggcctgatt
    20041 tgcttcacat atctcatcaa gagcagtcaa ggtttcagtt gaataatagc ctaaataggt
    20101 agcatctaat aagaaacgga tcgacaatgc atcgtctaaa aaggcagcat gcttatcttc
    20161 cggcgcgtct gttgccgcat atttcgtctc taatgagtga actacaccaa tttcacccgg
    20221 aagttcgttt tctttgaaat agttcactac acgggcatga gcaaccatca tattatgaag
    20281 acaggcgacg atctttgtaa aatcatattt gattcctggt gggaaaacac ctaacaaata
    20341 ttgattggtt gcaactggat agatttcatt aaaggtactc cacaccttga cttctttgaa
    20401 ttcatgaaaa caaaaaatgg cataggaaac aaatgcttct attgtttctc gattcaaaaa
    20461 atctccatgg tcaaacaaac gtttaggggt atcaaaatga tgcaacgtca caaacggcgt
    20521 tacgtttctt ttcttacatt cttcgaacac acgatgataa aaagcgattc cttctggatt
    20581 tggtttccca gctccatcag gaaaaatgcg gctccaagca atggataatc ttagaccatt
    20641 tacaccaaaa cgttcacaca attcaatgtc ctttgcatat tgatgataaa aatcacttgc
    20701 cggatcggga ctgaatcgcc cttgttcctc taaaaagtca tcccaagcaa cagcgccttt
    20761 gccaccttcc ttagtggctc cttcaacctg ataggctgct gtggctccgc caaaaataaa
    20821 atcttctggt aatttaagca tgtacatctc tcctcaaatc tataatcagt tttcgctctt
    20881 tctaactgat tgcattgtaa acgcatccaa aacatttgtc aacaaaaaca aacacaaata
    20941 atcataaaaa ccagaaaaca gcaataaaat ctattgtaac aaacataaaa accaccctaa
    21001 atgtttactt ttgttcgaat tagattgact gttgtccgtt tatatgatag attatcctta
    21061 aattagaggt gaagacaatg ttgaaaaaag aaagattgct tactatcgta gaaatggtca
    21121 ataaaaaagg aatccttacc gttaatgaaa tcatcaatca attagatgtt tctgatatga
    21181 ccgttcgcag agatttagat gaattagaaa aatcgggcaa gctgctgcgt gtccatggag
    21241 gggctcaaag tctttcctac actttagatc aagaactttc tcacactgaa aaagccactc
    21301 tgcagattca agaaaaaaaa gaaattgctg ccacagcagc ctccttgatt cacgagggag
    21361 aaacgatttt cttaggacca ggtacaacaa tcgaactatt ggcgacgcat ttactgaaca
    21421 agaaaattcg gatcattaca aataattatc cagtttttga tattcttgct caagcagatg
    21481 gaccagaaat tctactgatt ggcggtgatt tccggaaaaa taccggcgca tttgtcggac
    21541 cgatcaccaa cgacaattta agaagactaa agtttacaaa agcatttatc agttcaaatg
    21601 gcgttcacaa cgaagcgatc tctacttaca gtaccgaaga aggagaagca cagcagatcg
    21661 ctctaaataa ttcccgtaag aaatatctac tcgttgataa taaaaaattc aatcgagatg
    21721 atttttatgt gttttataat ttgcatgatt tcgatctgct cattacggat cgccagatct
    21781 cgcgggaggt tcaagagcac tatgcacaat atgtcgatct gcaaattgct gattcaaccg
    21841 aaaataccac tataggagcg taactatgaa agggattata tttgatttca acggaacaat
    21901 gtttttggac tctcatctgc atgaagctgc ttggctgcat atgatacatg atcatacaaa
    21961 agatggttta tcagacgagg atattttaaa aaatattcat ggtcggacga atacggagat
    22021 tttgaaacat ttcatttcag agcatttgac cgcctctgag atcgccgtat tgtcagaaga
    22081 aaaagaaagc tattatcgta tgctttgctt gcaaaatgaa aacgagcttc agttgacaaa
    22141 agggcttgaa gcgactcttg accagctgac tgaccaacag attccattaa cgattgccac
    22201 ggctactgtc aaagagaatg ttgcttttta ttttgatatt ttcgatttag cccgctggtt
    22261 tgactgggac aagattgtct atgacgacgg ctctttccct gggaaaccgc aaccggatat
    22321 ttttcttaaa gcggcagaca aactgagctt agctccggct gattgtcttg ttatcgaaga
    22381 tgcttactca ggattacttg ctgccaaacg tgcggggatc ggaacaatta ttgcaattga
    22441 tccattcggt aaaaacaaaa cagtatttga acaggcacag ctaaataaag atggaaccat
    22501 caaagatttt actggttttt ggacagagca cctgtctgcc acatccgtta attcttaaac
    22561 gattcaacta actaccaaag aaaagcaact gaaatgcaca cctcctttta taaaaaactg
    22621 ccagtgaaac gacatgaaag atacactgct atatccttag tgaaatatag cagtgtatct
    22681 ttgacttttt cattctttta accatatgac gcgttcatct ctatctataa ttaacattaa
    22741 aaatagtgtt ttcacattat ttctcaaacc aatcttaaaa atcatcagga gtactttttt
    22801 gatatacttt tattaatgct gggcgtaatg gttaaaagat ttccactagt tcctctccac
    22861 caagctgagt tgaaaaatgc ttatctgtaa acaacataga caaagaggaa ataaacgttt
    22921 ccgtatcttt ctctgtccca aggatattaa ttaattttcc tactaggtaa gtatcattaa
    22981 acttaagatc tgccttcaca tcaaaagttt tgataaaagc ttttatcatg cgtacaattt
    23041 tgtctttatc gccataattg atttcaccac tcatatcaat atagtcacta atccctgaag
    23101 aaataatggc aactgcttct tctttcgcct tatcagataa ttcatttaat gactgggatt
    23161 gattgatgac ttcttgagtt ttatctgtta cgtaatctct cattatccta tcggcatact
    23221 ccgcaaagta atcttgaaaa aattcttcta cagggtaacc aacttcgttt tcccaaccag
    23281 gagaaatttc attatcagta cttattaaat ctattaaaaa ccaacccgtt ctctgagtgt
    23341 tgattctata atatttttaa tttcctatat aaatcaaaaa tattatagaa tcaataagct
    23401 aattaagaaa caaactttag aaactctaga agaacttctg aatttatata gaagtgtgta
    23461 gctacttact aaatatacaa aagt
//
LOCUS       DAHHZJ010000027        23396 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.028, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000027 DAHHZJ010000000
VERSION     DAHHZJ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.028"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..788
                     /locus_tag="NBL02_000869"
     CDS             <1..788
                     /locus_tag="NBL02_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCD2571574.1"
                     /translation="ALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKYYI
                     SLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLSR
                     RALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIED
                     LNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHK
                     DGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            974..1645
                     /locus_tag="NBL02_000870"
     CDS             974..1645
                     /locus_tag="NBL02_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCD2571575.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            1704..2144
                     /locus_tag="NBL02_000871"
     CDS             1704..2144
                     /locus_tag="NBL02_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571576.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            2156..2824
                     /locus_tag="NBL02_000872"
     CDS             2156..2824
                     /locus_tag="NBL02_000872"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCD2571577.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            2888..3511
                     /locus_tag="NBL02_000873"
     CDS             2888..3511
                     /locus_tag="NBL02_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HCD2571578.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            3567..3953
                     /locus_tag="NBL02_000874"
     CDS             3567..3953
                     /locus_tag="NBL02_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HCD2571579.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            4166..5557
                     /locus_tag="NBL02_000875"
     CDS             4166..5557
                     /locus_tag="NBL02_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HCD2571580.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            6168..6536
                     /locus_tag="NBL02_000876"
     CDS             6168..6536
                     /locus_tag="NBL02_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCD2571581.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            6716..7657
                     /locus_tag="NBL02_000877"
     CDS             6716..7657
                     /locus_tag="NBL02_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCD2571582.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            7726..8457
                     /locus_tag="NBL02_000878"
     CDS             7726..8457
                     /locus_tag="NBL02_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HCD2571583.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            8601..10781
                     /locus_tag="NBL02_000879"
     CDS             8601..10781
                     /locus_tag="NBL02_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCD2571584.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            10801..11283
                     /locus_tag="NBL02_000880"
     CDS             10801..11283
                     /locus_tag="NBL02_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HCD2571585.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            11299..12060
                     /locus_tag="NBL02_000881"
     CDS             11299..12060
                     /locus_tag="NBL02_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCD2571586.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            12191..13525
                     /locus_tag="NBL02_000882"
     CDS             12191..13525
                     /locus_tag="NBL02_000882"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCD2571587.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            13832..14317
                     /locus_tag="NBL02_000883"
     CDS             13832..14317
                     /locus_tag="NBL02_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HCD2571588.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            14310..14993
                     /locus_tag="NBL02_000884"
     CDS             14310..14993
                     /locus_tag="NBL02_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCD2571589.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            15119..17764
                     /gene="polA"
                     /locus_tag="NBL02_000885"
     CDS             15119..17764
                     /gene="polA"
                     /locus_tag="NBL02_000885"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCD2571590.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            17809..18645
                     /gene="mutM"
                     /locus_tag="NBL02_000886"
     CDS             17809..18645
                     /gene="mutM"
                     /locus_tag="NBL02_000886"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HCD2571591.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            18609..19238
                     /locus_tag="NBL02_000887"
     CDS             18609..19238
                     /locus_tag="NBL02_000887"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCD2571592.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            19290..19622
                     /locus_tag="NBL02_000888"
     CDS             19290..19622
                     /locus_tag="NBL02_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCD2571593.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            19934..20425
                     /gene="nrdR"
                     /locus_tag="NBL02_000889"
     CDS             19934..20425
                     /gene="nrdR"
                     /locus_tag="NBL02_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HCD2571594.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            20448..21821
                     /locus_tag="NBL02_000890"
     CDS             20448..21821
                     /locus_tag="NBL02_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HCD2571595.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            21821..22747
                     /gene="dnaI"
                     /locus_tag="NBL02_000891"
     CDS             21821..22747
                     /gene="dnaI"
                     /locus_tag="NBL02_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HCD2571596.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            22876..23316
                     /locus_tag="NBL02_000892"
     CDS             22876..23316
                     /locus_tag="NBL02_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCD2571597.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         7959 a         4022 c         5071 g         6344 t
ORIGIN      
        1 ttgctttgat tgatagtaaa ttcatcaaag ttcctaaatt gaagtcatta gtaagaatta
       61 agcttcatag acaaccaaaa gggattatca aatccgctac aatatcacgt cattctagcg
      121 gtaaatacta tatttctttg ttgtgtaaag aagaaattat cgaattacct aaaactaatt
      181 ccacaattgg tattgactta ggtattatgg actttgcgat tctttctgat ggacaaaaaa
      241 ttgataataa taaattcacg tccaaaatgg aaaagaaact aaagcgtgaa caacgcaaat
      301 tgtctaggcg tgctttattg gctaaaaata aaggtaccaa tctatttgaa gctaaaaatt
      361 atcaaaaaca aaaacgcaaa gtagctagat tgcatgaaaa agtaatgaat caacgtactg
      421 attttctgaa taagttgagt acagaaatga tcaaaaatca cgatattatc tgtattgaag
      481 acttaaacac aaaaggtatg ttgcgaaatc ataaattagc taaaagtatt tctgatgttt
      541 cttggtcaaa gtttgtgact aaattacaat acaaggctga ctggtatgga cgaaaaatca
      601 tcaaagtaga taaatggttt ccgtctagtc agatttgttc agagtgcgga cataaagatg
      661 gcaagaaatc tcttgaaatt cgagaatgga cttgtcctgt ttgtcatact catcatgatc
      721 gtgatatcaa tgctagtatc aatattctga ccgaaggtct aagactacat tctatgggat
      781 tggcttagat ccaatcatca agaaccgtag gaactacggg gatagcttgg taaataagag
      841 acaccgctgt tagtaaagaa atacgctatc aagtatgctc tattcccaag aagctcccac
      901 ttcaagcgtt aagaaccgtc aggtttagct aagtggtgag tagttcacaa atgatgaaaa
      961 aagggggaga aaaatgaatt ttttagttgt tttaaaagat actctgattg agcgatctac
     1021 atttctatat gttcaattgc aggaaaataa aacattgctg catttttcac cagagttcca
     1081 tctagagggg ctaacattag gagaaatcta tcaagcagaa gggctgtcgg ctgttttgca
     1141 cttttttaag gtagaattgg aattaccagt aaaagattat atcgagcttt ctcttgaatc
     1201 ttgggatcga gcagtggttg aactgtttcc agaaggttta atgatcgata caaacgatgg
     1261 gaagatccat ctgactgcag cagaactgca aaaacaaatg cgttatcaaa tagatgacga
     1321 aaattctttt tctattttta gacgtcagca aaagattcta aaaagctttt taaaagtgat
     1381 cagtaaaaaa cggaatctga tcaaaacgac acagctgttg aaaaaatatc caggtctgct
     1441 tcacacgtct attcaatttc ctcaattgat tacgatgatc catcgtttta tacgtatgca
     1501 acagctacca tctaagaagc tgtttcttcc attgacggac acatttcgag tagtcaacag
     1561 agaagataaa aagaaagtga ttgtgattga ctttaccaga aatcggaatg tccttcacca
     1621 agttgcaatg gaaaaggcca attaaggctt tttctttttg gatttttttg tcataataaa
     1681 tattagatga tggaggagat aatatgatgc aaacatggtt agaaaggcta atacgatttc
     1741 agcagaacta tcacaaactt atgcagggaa gatatgcgaa atttgatcag ttgaatcgcg
     1801 gtctattgat cgctgcgctt gtctttagtt tgctaagtcg ttggttgccc tatagaatcg
     1861 gacaattgtt gttcatcgtc tttttcggat ggatgatatt tcgtttccta tcaaaaaaaa
     1921 tctaccccag actaaatgag aatcaacgtt atttgaaata tcttgataga gtcaaagaaa
     1981 aatatcatca gataaaaaac aaaaagcaag tcaaaatgga tcgaaaaagt tatacttttt
     2041 ttgagtgccc gaattgtcat caaaagcagc gtgcacctaa aggaaaagga cgaatacgtg
     2101 tgacatgcaa aacatgtggt ataaaattcg aaacgaatgt ttaagggaga aaagaatgaa
     2161 attagtatta ttacgtcatg gcgaaagcga agcaaatttt gaaaattact ggacaggatg
     2221 gctagatgtc gccttaacag aaaaaggaaa agaacaagca gcagttgccg gtcaaaaaat
     2281 gcttgaagcc ggacttcaat tcgatgccgt ttatacctct gttttgaaac gtgcgattct
     2341 taccggacaa gtcgcactag aagaaatggg tcagttatgg cttcctataa taaaaacatg
     2401 gcgtctaaat gaacggcatt atggggatct tgtgggtaaa aacaaagata agatgaaaga
     2461 aatatacgga aaagatcaag taaaaacatg gcgcagagga tatacggaag ttccgccgtt
     2521 aaccgcagaa aatagatttg accgcagata tgatcacttg gatccgcatt tgatcccata
     2581 cggagaaagt ctggaaatga cagtgaatcg gatcattccc ttatggcaag atcatctagc
     2641 accaaaacta aaagatcggc aagatttact agttgtgggt catggtaata gcatccgagc
     2701 actgacaaaa tatttagaag atgttcctga agatcaaatg gatacgatcg atatcccaaa
     2761 tgcacaacca atccagtata cattagcaga tgatttgaca attctggaca aaaaaatact
     2821 ttaaagaaca cctttatcta aaagtgagag atagaaaaaa gaagcaaagg aatggaacaa
     2881 aaggaacatg aaaaattata tatgggactt tgatggtaca ttgtttgata cgtatcccgc
     2941 aatggtagat ggtgcatggc aagcactaaa agattttggt atcagtatgg acaaaaaaga
     3001 aatctacttc aaaatgaaaa agtattctac tagttatttg atcaatgaat caaacttgaa
     3061 tgccagagag tttaatgaat tatttcaccg atatgaaaaa gaaagtacgg aagtttcccg
     3121 tccatttcca gaaacgaaac aagtactgga aatgctaaaa gacaacggcg gtcgtcattt
     3181 catcctgacc caccgtttga cagaatcaac atggggcctg ttaaaagaac atcgattggc
     3241 acatctgatt gaggaagtag tagggatcga tcaggacttt cctcgtaaac ctgatccagc
     3301 atctttaaat tatttgatcg atacttttca tttagaaaga acagacacga tgatgatcgg
     3361 cgatcgtcgt ttagatatcg aagcagggaa aaatgcagga gtcgctactt gtctttacga
     3421 catcgatcat tttctgggtg agatcccggc agattatgtg gtaggaaatc tgaatgaaat
     3481 ccttaccttg cagagcatca ttaaaaagta gtatcccctt tcaaaggaga tatgttataa
     3541 ttttcataaa ggagtgataa aattatatgg aaatcagaag acaaaaagaa gttgtagaag
     3601 catttcatta tgatatgaga ttgccgaacc aagaagtaga aacagatttg aaagtaggct
     3661 tctcgccttt aaaagaaacg atcgaaaact atccgaaaga acatagcatc gtaggagcac
     3721 gcttagagtt ccgtttagtg tttgatacat atgttttatc tggaagtgtg agtcagatca
     3781 atcatatcct caatcggaaa attgaaggtc aaaatgatgt cacacaagaa gaagtggacg
     3841 agctagtagc accgttattc gatatcgtac gccgaatgtc ttatgaggtg acagagatcg
     3901 ccttagatca gcctggggta aaattgaatt ttcaatcaga aaaacaagaa tagacagtaa
     3961 aaacatacct gcgatagatt aaagctatca caggtatgtt tttttattac taattgtaag
     4021 caaaaagaat gtcatttcga taaaaatcgt ctaaaatata aaagtcactg aaaaaaattt
     4081 tttcaggagg atggttgaag aatcattctc gggaaccttt acttccttcg atggacttgt
     4141 cattaataat atgaggagga gaaaaatgac aacactcatc acagttttag tggttctagt
     4201 ttttgcggca ttattatatg tgttctatcg tatgcaaaag cgccatacaa aattttcaac
     4261 acgcgtcttt acagcattag gagttggtat cgtctttggc ggtctgcttc aattgatctt
     4321 tggtgtagac gacaaagtcg ttactcaatc aatggaatgg atcgggattg taggtcaagg
     4381 atatgtatca ttcttgcaaa tgctagttat gcctttagtg tttatttcga tcattggtgc
     4441 attcacaaaa atgaaagttt ctgaaaagct agggaaaatt agtgcgactg tattgacttc
     4501 tttacttgga acaacagcta ttgcagcatt gatcggaatt ggttcaacaa tgctgttcgg
     4561 actccaagga gctaaattca cacaaggagc tgcagaaaca gcacgaatct ctgaactggc
     4621 aacacgtcag gaaacagttg aaaacttatc gattccacag cagattttat cctttatccc
     4681 taaaaacgtt tttgctgatt tagcaggtag tcgtgcaaca agtacgattg gagtcgttat
     4741 tttcgcggca tttgtcggaa tcgcttatct aggcgtgaaa agaaaagcgc caaaagaagc
     4801 agcattcttt tcagatttag tcgacagtct gtataaaatc attatgcgga tcgtgacgct
     4861 tgttttgcgt ttgacacctt atggagtatt tgctttgatg acaaacgttt tggcaacaag
     4921 tgattttgca gctatcgtca atttaggtaa atttgtcgtt gcttcttacg tagcattgct
     4981 tgctgttttg attgtccact cactgatttt gattggtgca aaagtcaatc caatcaacta
     5041 tttcaaaaaa gcctttccgg tattgagctt tgcgtttact tcacgttcaa gtgctggagc
     5101 tttgccattg aatatcgaga ctcagacaaa agctttagga gtagatgatg caacagctaa
     5161 tttctcagca agttttggtt tgtctattgg acaaaacggc tgtgctggcg tttatccagc
     5221 tatgttggca acgatcgtcg caccagcagc tggagtgaat gtctttagtt tggaatttat
     5281 tttgatgctt gtggcaattg taacaatcag ttcttttggt gtagctggag tcggtggggg
     5341 cgcaacattt gcttcactga tcgttttagg ggcaatgaac ttgccagtag caatcgttgg
     5401 gttagttatc tctgttgaac cattaatcga tatggcacgt acagcagtaa acgtcagcgg
     5461 tagcatggta gctggtatcg tgacaagtgc acgcatcaaa gatttagatc gcgatgtcat
     5521 caatgatgat tctaaagtga tcgatgtgaa tgcatagtca aaaagaggtt gtgatagaag
     5581 tggtcagctt taagaaataa gaagaagttt cctaaaaata gtttctcaaa tttttgtgaa
     5641 atttcggctt atttccgaga agctacttct gaaacaccgt gaataagagg ttgtgaaaaa
     5701 gctgccctgc atcaagtaat aagaacagcc aaacaaaaat agttcctcat attttacgtt
     5761 tggttgagct tattaccgca gatgcaagct tttgaacacc gtttaagaaa gaggtcgtga
     5821 gcctgccgtt tcactctgag tcacaaagag caatttcaaa aaatagcttc tcatgtttgt
     5881 tgaatattgt gtcttgtgac cgaggagttg tcaggagaac accgtttacc agaggttgtg
     5941 acttgttttt agtcacgacc tcttttttta tttattccat ataaatacac attatttatt
     6001 ccgaaaagtg aaagcgcaaa taacaaaaac aaccaactta tttattatta aagaaaagcg
     6061 tatttaagta ggacgattct gttgagagct ttcgtagatt ttcccatttt cctatggtaa
     6121 actgacaaag ttgagaatgt tagaacagat cgtttaggag gaaacagatg tttacgaatg
     6181 aaataaaaat catgctttat gtggacgatg tagaaaaaaa tgcaacgttt tggagagcag
     6241 ttggctttgc ggaaaaagat cgtcaagaaa tggatgggac gcttataatt gaagtaggag
     6301 ttgaggacag tcaaacctca tttctgttat atgatcgagc atttgttgag tcccattcac
     6361 ctggcacagc tactagtgcc ccatctttga tgttttacag taaagatatc tttggactat
     6421 ataaaaaaat gcagacgcaa tcagtcacat taggtgatat ggtacagctt ggagaagaat
     6481 atgtctttaa ctttgctgat cccgatggca actactttgc ggttacaggt aaataataga
     6541 aataaagact ttgtttttct gtgcgaaaca ggaaagcgaa gtctttttaa ttcaaataat
     6601 aatattatgt aaacaaaaaa ctttccatgc gtaattatac acaagcaaat aaatcgtcaa
     6661 atgcgaacgc cttctttata ctgtttagga acgaaaagaa ggaaggaaga aatacatgga
     6721 ttatcaagta ctgctttatt acaattatac aaaaatcgaa gaaccagagg aatttgccaa
     6781 aaatcattta gccttttgca aatctttgga tcttaaaggt cgtattctag tagctgaaga
     6841 aggaataaat ggtactgtgt ctggaaccgt tgaaaacacg actgcctata tgaaagcaat
     6901 gaaagctgat cctcgatttg ctgatacgat gttcaagatc gatccaagcg aaggacatgc
     6961 ctttaagaag atgtttgtaa gataccgtcc agagcttgta agtttaaagc tagaagagga
     7021 cattgatcca aaccaagtga caggtgccta tctttcaccg caagaattta aagaagctat
     7081 tttagatgaa cagacagtgg tgattgatgc acggaatgat tatgaatatg acttgggaca
     7141 ttttcgaggg gctgtacgcc cagatatacg cagttttcga gagcttccag actggatccg
     7201 cgagcacaaa gaagaattta tggaaaaacg ggtagtgacc tactgtactg gtggcattcg
     7261 ttgcgagaag ttttctggtt ggcttgtccg tgaaggattc aaagatgtcg gacagctgca
     7321 tggcggtatt gctacttatg gtaaggatcc tgaagtccaa ggagaattat gggacggcaa
     7381 gatgtatgtg tttgatgaac ggatcgctgt cgatatcaac caagtagata aacaaatcgt
     7441 cggtcgcgac tggtttgacg gaacaccatg tgaacgatat atcaattgta gcaatcctga
     7501 gtgcaatcgt cagattcttg cttccattga aaatgaagaa aaacatttag gagcatgtag
     7561 catagagtgt gcgaaacatc cgcgcaatcg ctatgttcaa gaccatggaa tctctccaga
     7621 agaagcagaa ctgcggttta cagctttaat taaatagaaa acaatagttt tgattgtcga
     7681 ccatttccaa tatgcgctat actagcgttg aggatggtga tccacatgaa atcattgatt
     7741 tatgctattg gggatgtcca tggcaaacat gagctttttg aaaaacttat tcactattat
     7801 gatccaaaga tccatcagct tgttttatta ggggatctca atgatcgagg tcctcgtaca
     7861 aaagaatgtt ttttattagg aaagcaatta gtcgaagaaa caggagccat ttacctccgg
     7921 ggaaaccatg aagaatattt tttgcaattt ctgcaatcac cggaggactg gtatgcgcct
     7981 tatatgagaa atggtggaaa agaaacgttt gaaagcctga ttcacccagg ggcatcggag
     8041 gaatactccc caactgaaat ctcgatgatg atccaatcga gatattcaga actgattaaa
     8101 tttttagtcc agcgcccctt gtattatgaa tgggaaaatt atctttttgt ccatgccggt
     8161 gtggatttga caaagaaaga ctggcgtaag acaagtctaa gagactttat ttggataagg
     8221 gaaccatttc atgaagggaa aaacaataca ggtaagacaa ttgtatttgg acatacgatt
     8281 acaccaatgc tccatggaga tatgcagaca acagacctct ggattcagga tcataaaatc
     8341 ggaatggacg gtggggctgt attcggaggg tcattgcatg gggttgtttt tgacaaggaa
     8401 ggtattcttc aagatattga atatcagaac aacgaaggac cttggcatgg aatctgacta
     8461 atggaaagaa tgaaggagct gtgactttgt tgcggctctt tttattttta tagagaatat
     8521 tgtttctttc agtagtttaa cccaggataa aaagtatgat agaataaaga agcaattttt
     8581 agcagaaaag aggcatttta atgacggaaa atttgaacca gacgatcatt cagcttgtac
     8641 aaaaagaact ttcagactat cgtcctaagc agttgactac tgtacttaat ttgttaaatg
     8701 aaggaaacac tgttcctttt atcgcgcgtt accgaaaaga aatgactgga agtcttgatg
     8761 aagtccagat tcgtgaaatc gaagagcggt atgcctatct tgaaaactta gaaaaacgta
     8821 aaactgaagt gattcggcta atcgatgaac aagggaaact gactcccgag ttagaagcag
     8881 aaatcaccca agccgtgaaa atgcaacaag tggaagattt gtatcgtcca tacaaacaaa
     8941 aacgacggac aaaagcaacg attgctaaag aaaaaggact tgaaccactc gcgaagtggc
     9001 tgatgcagct tacagatgga gaggtgcaat ctgaagcaga aaaatatatt gacaaagaaa
     9061 aagaagtctc ttctgccgaa gaagcgttgc acggtgctca tgagattatt gctgaacaag
     9121 tcagcgataa tgcaaaattt cggacttgga ttcggtctta tacgtataac aaaggtatgt
     9181 atgtcagcaa tgtcaaggat gagcaagcag atgaaaaagg cgtatatgaa atgtattatg
     9241 actttgcaga acccgtccat aaaatggttt ctcatcgaat attagctact aatcgtggag
     9301 aaaaagaaga tattctgaaa gtattctttc aagtagatga agcagcaatt ttagcttact
     9361 tggatcgcca aatagtcaaa aatcctgcct ctccttcttc ttcattcgtc agagaagcct
     9421 atcaggatag ttataaacgc tttattcagc cagcaatcga gcgagaactg cgcaatgaat
     9481 tgactgaaaa ggcagatgag caagcaatcg ctattttcgg cgaaaacctc cgtaatcttt
     9541 tgttgcaacc gccactaaaa ggtaaagttg tcctgggatt tgatccggct tatcgtactg
     9601 gatgtaaact agcagtcgta gatgcgacag ggaaagtttt agcaatcgaa gttatttatc
     9661 ctcataaacc tgcagcgcaa gcaaaaagag aagctgctgg accagctttt atccaattaa
     9721 ttaataaata tcaagtagat atggtagcta tcggaaatgg tacagccagt cgggaatctg
     9781 aactttttgt ggctgagcag ttgaaatccg ccgaccataa aacctattac gctatcgtca
     9841 acgaagcagg cgcttctgtt tactccgcta gcgaaattgc acgaaaagaa tttccgcatc
     9901 tacaagttga agaaagaagt gctgtttcta ttgcccgtcg cctgcaagat cctttagctg
     9961 aattagtcaa aatcgatcct aaagctgtcg gagtaggcca gtaccaacat gacgtttctc
    10021 aaaaacgttt ggctgaacaa ttggattttg ttgtggaaac agcggttaac caagtaggtg
    10081 tggatgtgaa tacagcaagc ccgcagctct tgcagcatat atcaggattg aataaaacaa
    10141 ctgcccaaaa cattgtcatt tatcgtgaag aaaacggaga attcaccgca cgaactcaat
    10201 tgaaaaaagt gccacgctta ggaccaaaag catatgaaca agcaatcgga ttcttgcgag
    10261 tccctggtgg gaaaaacatt ttggacaaca caggtatcca tcccgaaagt tattctattg
    10321 caaaagaaat attaacttca gtccaacttt cagaaaaaga gcttggtaca gaagaagcag
    10381 tagaaaaatt gacacgatta tccgtagaga aactggctga aagcctatca gtcggagaag
    10441 aaacgttagc cgatatttta gctgggctga cacaacctgg acgagatatg cgtgatgaga
    10501 tgccagcgcc cttattacgt acagatgtat tgagtatgga agacttgaaa ccagggatgg
    10561 aattgactgg aacggtccgt aatgtcattg acttcggcgc atttgttgat atcggtgtga
    10621 agcaagacgg acttgtccat atatcgaaac tcagcaaaaa attcgtcaag catcctacag
    10681 atgtcgtgtc agtaggagat atcgtaacag tttggataga acaagtcgat acgaaaaaag
    10741 gcagaatcag tttgacgatg ctttcccctt atgaagagta acaatgagga gtaacaaaaa
    10801 ttgacacaag aagaactgca gcagttagta gaagagatat cattaaaaag cttccagctt
    10861 cctttcaagc accaagcaat gttcaatcca cgcttacgta caactggcgg gcgctatcat
    10921 ttaaacgatc atcatttaga ttttaatgcg aagctatttg aagagtcaga cgaagagacg
    10981 attgcaggaa tcatcaaaca tgaactttgc cattatcatc ttcatctagc tggaagaggg
    11041 tatcagcata aagatatgga tttcaagtat cttttgaaaa aaacaggagg gttgcgctat
    11101 gcaccagcct tgacgaagaa acagataaag atggagtatt atcgatgcca agattgttct
    11161 gccacgatct gccgcaagag aagaatcgat gtacgcaaat atcgttgtgg aagatgtcat
    11221 ggagaaatca aatggattga gacaaaaaga gaagacttta atgaagcaca aaaaatcaga
    11281 tgaaaggata gaaggccttt ggaaaaaagt gagcagagga atctaaaaga ctggaatcag
    11341 ctattaaatt ttttagccgc acctgaagag ggaaattttg aggaactgac ggcacctaca
    11401 cttattttag ctggaaactg tcttcctatc ttagctgata aagcagcaca aatgtattta
    11461 aatggccaag tagatcaatt gtttttggtc ggaggggtgg ggcatgcgac tagaatcctt
    11521 tatgagaatt ttgaaaagca aggatttcat tttgaagatg gcatgagtga aagtgaaatc
    11581 tgtcgtcagt atctaaaaga agtctatgat ttgcccgata aggcttttct gatagaatcg
    11641 aagtctacaa attctggaga gaacgccact ttttcactgg agattcttca ttccttagga
    11701 gctgtaccag aaaaaatctt attgatgaat gatccgacac tgcaacgcag gactcgagca
    11761 acttttgaga aagtatggca aaatgaacaa actattttcg cgaatgctgt gccatttgtg
    11821 ccggaaatcc ttcacttttc tgaagagatc atctttacag caaaagaact gaatcatcaa
    11881 tggcctaagg aatatttcca tgcgctagta ttaggagaaa tggaacgtct ccatgacgat
    11941 gagaacggct atggtccgaa aggaaaagat tttattcctc acattgatat atcagaggaa
    12001 gtttggtctt cttacaccag aattaaagag tcaataaaaa cggatttttc tcgtacttga
    12061 ccattttagc aagcgaatcc tcctttccaa ataaatttgg ttgaaatttt caatcgttta
    12121 taagataatg aggagcgtgt tgaatgtaat agctaaaaga atggacgcta gataagagaa
    12181 gaggattcaa atggaaaatc aaaataaatt gtatcatttt gttggtatca aaggttcagg
    12241 aatgagctcc ttggctcttg ttttacatga acaaggtcta aatgtccaag gatcagacat
    12301 tgaaaaatat ttctttacac aaagagattt agaaaaagca aatatcacga ttctgccatt
    12361 taacgcagat aatgtaaaac cagggatgac gatcattgca ggaaatgcat ttcctgattc
    12421 acatgaagaa atccagcgag caaaagaatt aggtttggaa gtgattcgct atcacgactt
    12481 tattggtcat tttatccaaa attatacgag tatcgctgta acaggctctc acggaaaaac
    12541 aagtacgact ggactgctct ctcatgtatt gagtggtgtc cgtccaacaa gttatctaat
    12601 tggagatgga acaggacatg gtgatccgca agcggaattc ttttcatttg aggcctgtga
    12661 atatcgccgt catttccttg cctattcacc agactatgca atcatgacca atatcgattt
    12721 tgatcatccg gattattata caagtatcga cgatgtattt acagctttcc aaacaatggc
    12781 tggacaagtg aaaaaagcta tttttgcgta tggtgacgat gcttatttac gcaaattaaa
    12841 agcaaatgtg ccgatttatt attatggtgt cacagaaaac gatgatatcc aagcacgaaa
    12901 catcgaacgc acaacttctg gttctgcttt tgatgtctat catggggatg aatttgttgg
    12961 gcacttcact gtaccagcat tcggtaaaca caatatctta aatgctttag gtgttattgc
    13021 agtagcttat ttcgaaaaac tggatctgaa agaagttgca gaagaaatgc tgactttccc
    13081 aggagtaaaa cgtcgattca gtgaaaagat cgtagcagac atgaccgtag tcgatgatta
    13141 tgcgcatcac cctgcagaga tcaaagcaac gattgatggc gcacgccaaa aatatccaga
    13201 taaagaaata attgcagttt tccaacccca tacgtttacg cgtacgattg cattgatgga
    13261 tgaatttgca gaagcacttg atttagctga taaagtttac ttatgcgata tttttggttc
    13321 ggctcgggaa gaacaaggaa acgttaagat tgaagacctt ggagcgaaaa tcaaaaaagg
    13381 tggagaagtc atcaaagaaa ataatgtatc tccattgtta gattatcatg atgcagtagt
    13441 aatcttcatg ggtgctggag atgttcaaaa atttgaacaa gcgtatgaaa aacttttaag
    13501 cagcacgacg aaaaatgtac tgtaatacaa ttactgcagt gtccttataa aaatagaaag
    13561 agggggagtg gcaaaaatct tgccaagccc ccttttttgc aacttatgat aaacggtgtt
    13621 caaaagcttg catctgcggt aataagccca atcaaatcaa aaacatgaga agctgttttt
    13681 tcttggctgt tcttattatt tgatgcagga cagctttttc acgacctctg taaactatag
    13741 gaatctgtta aatcaaggga gcagaacttg ccttttacgc tacttttggt acaataatgt
    13801 atgattaaaa ataaactgga gggtttctat cttgaacata ggtaagccga gaaaactagg
    13861 aaaaacaata aatgaaatcg aggaaggcga ctcgttatcc ttaacagaat ctattgaaga
    13921 taaccagctt ttgctgtatt tgggattgac gaatgatgca aatcctttgt atatccaaca
    13981 tgactttgca caaaaaacag aatatcaaca gccaatcgtt ccatcagtga tgctgatggg
    14041 gatcatcaca agtgcaattt caaagcatct tcctggacca ggatcacatg ttgtcaattt
    14101 ttctgtcaat tttattgaac cggttttcca ttatgaaaca ctgacttttc agtttgaggt
    14161 cataaaagta gataagatga aggatgtggt gacgatttct gtagaagcgg agaatacgaa
    14221 cgaagatcgt gtgttggatg ctgtcgttat ggtccaaccg ccacaagttc ctgaattgga
    14281 aatagcaatc gaaagtgaag gtgaattgaa tgaataatcc aagtacatca atgtcaggta
    14341 tcaatcgttt ttatgcaaaa gtttatggat ttttaggtat gggacttgcc cttagtgcag
    14401 tcacttctta cttggctctt tgggtatttc ctttgcaatt agcaactttt atcaataatt
    14461 ttccactagg ctttgtgggt ctgtggatcg ttgaactgat tttagtaatg gttctaggcg
    14521 taaaagcaca aaagaatccg agcttagcaa tcggtggatt tattgcctac tctcttttga
    14581 atggcataac attagcggtt actcttgcat actacgatat tggaacagta acgcaagctt
    14641 tcttgacagc tactttgatg ttcatgggga tgtctgttgt cggagtaaca acaaaaagag
    14701 atcttagcgg ggttggaaga gcaggacagg cagctttaat cggagtgatc attgcactag
    14761 tcttgaacat cttcattttg agaagtagcg tagttgagct gttcatctcc ctactgttag
    14821 tcgtcatctt tgcagggatt acagcttatg ataaccaaag catccgtaat atctatgtcc
    14881 agacaaacgg aacagcacat agtggaattg ctgtattcat ggcattgaag ctgtatttgg
    14941 actttatcaa cttgttcttg gcgtttctga gaatctttgg acggaacaac taaaaaagaa
    15001 aaaaattcat cagttggggc tataccgaat tatttggtat ggtcccgctt tttcttttcg
    15061 cattccgcag gattctttgt tagaatgaat aagaacttta cagtaaggag ttaagaagat
    15121 gacaaaaaat aaattattac tcgttgatgg gaacagtgtg gcttttcgcg cattctttgc
    15181 gcttcacaat tctcttgaac gtttcaaaaa tagaaacggt cttcatacaa atgcaatcta
    15241 tgcattcaat acgatgtttg aaaatgttat gcaaaaagag caaccgactc atgttctggt
    15301 tgcgttcgat gcaggaaaaa cgacttttcg gacagaaatg tatgcggagt acaaaggtgg
    15361 aagatccaaa acacctggcg aattcaaaga acaaatgcca tatatacgtg atttgttgac
    15421 aggactaggg gtacagtatt acgaattgcc caattatgaa gcagatgata ttatcggtac
    15481 tttggcagaa aaagtagaca aagatcaatt tgatgtcgtc gtgctttcag gtgatcggga
    15541 tttgactcaa ttagctagta aagaagtaaa agtggatatc actgtaaaag gtgtcagcga
    15601 tatcgaaagc tatacaccag aacatgtagc ggaaaaatat gatgggttga cgccaaaaca
    15661 gattattgac atgaagggac tagccggaga tgcctctgac aatatccccg gtgtgactaa
    15721 aatcggtgaa aaaacagcta tcaagctatt gaaacaatat ggctcagtcg aaggaattta
    15781 tgaacatatc gatgaaatga agcaaagcaa aatgaaagaa aatctgataa atgacaaaga
    15841 gcaggctttc ttatcgaaaa aattggcgac catcaatact tcagcgcctg tggatgtctc
    15901 tatcgactca ttgaagtatg aagggaaaaa tctcgataaa ctcgttccgt tttataaaga
    15961 aatggatttc aatcagttcc tttctaaatt gaatatcgta gaagaagaag taaaaatgga
    16021 tgatattttg tttgaagtcg ttcatgaatt caaagaggaa atgtttacta cggatatggc
    16081 gttgtacgtg gaaatgatgg gcgacaatta tcatacagag gaaatcgtcg gagtagcgtg
    16141 ggggacagaa aagaaaattt atgtcacgaa cgatctatct gtattcgaaa accgagcgtt
    16201 ccattcttgg atcactgatc cgactcggct gaaaaaagta tatgatgcga aacgaactta
    16261 tgttgcttta aaccgctata ctggaaaaac gaaaggaatc gattttgatg tcttgctagg
    16321 tgcatacctt ctcgatacga atgaaaaaag cacagatatc gaaggaatag ctgctcatta
    16381 cggatacaac gatatccaat cagatgaagc tgtatacggc aaaggggcga aaaaaggttt
    16441 accagaagaa gaggaactct tttttgctca tttagcgcga aaagtagctg caatcaatgc
    16501 gttgacacca aaattgagcc aagaactggc agaaaaaaac caagaagatc ttttccggaa
    16561 aatggaactg ccattgtcta tcattttggc agagatggaa ataagcggga tcaaagtaga
    16621 tgcgactcgc ttacaagaaa tgaagggcga gttctctgct cgcttaagag agatcgaaca
    16681 aaaaatctac gaagaagccg gcgaagaatt caatttgaat tcaccaaagc aattaggagt
    16741 gatcttattt gaaaaaatgg gattacccgt tatcaaaaaa acaaaaactg gatattccac
    16801 agccgtagat gtactggaac agcttcgtga acaagcacca atcgtagagg atatcctcac
    16861 ctatcgtcaa attgcaaaaa ttcaatctac ctatgtcgag ggactgctga aagtgatcca
    16921 atcagatgga aaagtgcata cccgttatgt ccagacattg acccaaacag gacgattaag
    16981 ctctgttgat cctaatttgc agaacatccc gattcgatta gatgaaggga gaaagatacg
    17041 tcaggcattt gttcctagag aaaaagactg gctgatttat tcttctgact attcacaaat
    17101 cgagttacgc gtacttgcgc atatctcaaa tgatgaacat ttgaaagctg ccttcctaga
    17161 aggtcaagat atccatgcca gcacagcgat gcgtgtattt ggaatcgaaa aggcagagga
    17221 tgtcacacca aatatgcgta ggcaggcaaa agcagtgaat tttggtatcg tgtatggcat
    17281 ctctgattac ggactttcac aaaatctagg aatcagccga aaagcagcac agcaatacat
    17341 cgatacgtac tttgaaaaat acccaggtgt caaagaatat atggaaagaa tcgtacgcga
    17401 agcaaaagac caaggctacg tcgaaacgct gtatcaccgc cgtcgatatc tgccagacat
    17461 caattcaaga aattacaata tccgttcttt tgcggaacga acagctatca atacaccaat
    17521 ccaaggaagt gctgctgata ttttgaaaat cgcgatgatc gaattggata aacgattaaa
    17581 agaaacaggt cttcaagcta cgatgctgtt acaagttcat gatgaacttg tcttcgaggt
    17641 accggaaaaa gaacttgaat cattagataa attggtgaag gaagttatgg aacaagctgt
    17701 ttctcttcat gtaccactga tcactgacag cagttgggga aaaacgtggt acgaagcaaa
    17761 ataaacattg tttaagacag agcaatataa tgaaggtgaa aagaaaaaat gccagaatta
    17821 ccagaagtag aaaccgttcg taaaggattg gaacggttag tagtaggaaa aacgattcaa
    17881 aaagttcagg tcttgtggcc caagatcatc gagcaaccag agacacccat ctttgaggct
    17941 tctcttgttg gcgaaaccat ccaatccatt ggacgtagag ggaagttttt aatttttcat
    18001 ctcgatcatt gtgaactgat ctcacacttg cgaatggaag gaaaatatca atttacgaaa
    18061 gaaaatacac caatcgataa acatacgcat gtacttttct tttttgagga tggaagccaa
    18121 cttcgctaca acgatgtacg aaaatttggt cgtatgacaa tcgtcgaaaa aggagcaagt
    18181 gcaacttacc gaggaatcat gaaattggga cctgagcctt tgccagatag cttcttatta
    18241 gcagatttcg caaatggact gaaaaagtcc cacaaagcaa tcaagccatt gctgctagac
    18301 caacgtctag tgactggtct aggtaatatt tatgtagatg aagcattatg ggaagcgaag
    18361 atccatccag aacaaccggc gaacacactg cgccccaaag aggtagaaca attacgtcta
    18421 tcgatcattg atgttttgga tcgtgcaata gaagccggag gtaccacgat tcgcagttat
    18481 ttgaatgcat taggagaatc aggcggattt caagtagcac tgcatgtcta tcagcaaact
    18541 ggcaaaccat gtatacgttg tggaacgccg atcgtgaaaa caaaagtggc acaaaggggg
    18601 acgcactatt gtccgaattg tcaaaggcta aaaatcgcaa agtaggattt gttttaggtt
    18661 tgacgggtgg aatagctacc ggtaaaagta cggcagccaa agtctttcag tctcatggat
    18721 ttcctttgat cgatggcgat gtgattgcca gagaaactgt tgagcctgga acgcttgctc
    18781 tgaggaaaat tgtatcagtg tttggccaag aggtattgca gcctgatggg caactagatc
    18841 gagggaaact tgggatgatt gtttttccat caaaggaaaa acggcaaaaa cttgatcagc
    18901 tattagatcc gtttatccga aaagcgataa aagatcaaat cgcctctcta agttccaatc
    18961 atcctttagt aattgtagat atcccattgc tctacgaagg tcattatgac cactatatgg
    19021 atgcagtagc tgtcgtttat actacgccag aaactcagct tcagcgtttg atgaaacgta
    19081 atcaactgac gctagaacaa gcgcagcaga gaattagtag tcaacttcca atcgaagaaa
    19141 aaaagcagcg tgcggatatt ttgttcgaca acaatggaac aaaagaaaaa cttgtgggac
    19201 aaatcgaaaa ttggcttttt gctaatcgct ttctttgagt atctgtgtca attttgctat
    19261 cctgagtata tagaaagtga gggatagcga tgggatcatt aaaaaatcaa aaggataaag
    19321 tcgtaggtaa cgcaaaagaa aaaatcggcg agatgctcaa taaagaaaat ctaatcgatg
    19381 aaggtcgtac gcaatcagaa acagcagaag aaaaagaaaa gcagtaccaa gcaagaatcg
    19441 atgaaataga agaccagcag gcagaagaag aagcgaagcg gcaagatttg actcaagaac
    19501 gaaaaaatcc gacacctgat cgtagtaaaa caccgctccc agaaagtgac cacattcccg
    19561 atgagcaaaa aatcgaggaa cagcgaatga aacactatac aggaatcgaa gagaagttat
    19621 aaaatagtaa aaaatcgaga tgtgacttga agcagatgag aaggtgttca tctgcttcaa
    19681 gttttttgtg ctagatgact aaatcagctt ttgtgtaagt tatttctcag taatcaaggt
    19741 gatcagtatt ggaaaattga aatttagtaa ggacagggga cacaattacg catgaaagta
    19801 gaaaaaattg ttcttatatc cgtgtttttt gggtaggaca gtcgcgtttg tgatttgttt
    19861 cgtgttataa tacaactagt tgtaaagaaa gacacatcct ttatataaaa tgcgatgcaa
    19921 gaggtgagac aaaatgagat gtcccagatg tcaacataat aattcacgag taatcgacag
    19981 ccgtcaagca gatgatggac gtgcgatccg tcgccgtcgc gaatgtgaga attgttcgtt
    20041 ccgattcact acatttgaac gaattgaagc tgctccgtta ttagtaatca agaaaaacgg
    20101 ggaacgcgaa gagttcaatc gcgataaaat tttaagagga ctcatccgtt cagcagaaaa
    20161 aagacctgta gctatggaac aaatggaaca aatcgtcaac catgtcgaaa accgcgtacg
    20221 cagtctcgga gaaaacgaag tatcgacgaa tctaattggt gaatatgtga tggaagattt
    20281 agttgattta gacgaaatcg cttatattcg ttttgcaagt gtttatcgcc agttcaaaga
    20341 tatgtctgtt ttcttaaaag agttgcaaga tatcgtggat aaagcaaaag aaaataataa
    20401 agagaataat aaagaaaaca attaagaaac aaaaaggagg catttccttg gaaaatgctt
    20461 ggaaagagct tcaaccaaaa aatatttacc aaggaagaaa agcggctccg ctgagagaag
    20521 acagcaagaa cgccttgctg tatctttatc agccactaat cggcggagaa gccttgagta
    20581 tctatttcac cctgttatca gaaatctctc aagaatctgg caaaggtcct gaagggatgc
    20641 atgcagatct gcttagtagt ctaggctgcg gtatcccgca attttatgat gcaagaaaaa
    20701 agctagaagg aatcggctta ctggatgttt attttaaaga agatagtgat ctgggacctc
    20761 gttttattta tgagctgtta gaaccactca ttcctaaagc atttttcaaa gactctgttc
    20821 tggcttttct tcttctggaa aaagttggtg aacgacgatt ccagaagctt acccaattct
    20881 ttgaacctaa aaggcataca gcagagggat atcaaaaaat cacaaaaaaa tttttagatg
    20941 tttatcgctt caatgaagca gtgtacgctg ctaatcaaga aaacatcgaa tcgctgaata
    21001 aatcttttga agaatctgaa tcagtagcat tacttgaagc agattcgaca ttcgattggc
    21061 agtttttgaa tgattggctg gcaaaaaaac atattgcaag tcttgatgat ccaatcatca
    21121 aacaagtcga catgtatcat cagctttacg gtttcgatga attgacactt ggcgaactga
    21181 tcagtcaatc ttatgacttt agtgaacaaa aagtatctga aaaagaactg caaaaagcca
    21241 ttttggcaat gcagagacgt ccggtgaagc aaacaaaaga aacagttgcg gtttctgaaa
    21301 acagtaatga accctcgtca aacgaacatt tgacaaacgc agcgcttcag ctgataaaag
    21361 aagcacagag cattccaccg atgaaatatc tggaagcaat caagtcagaa aaaggcggat
    21421 atgtttctaa acaagaaaac tggttactgc aagatcttgt ttctcaatca ggtctttcca
    21481 gtagcgtgat caatgttttg ctgaattatg tactagtgat caagaataat gctagcttaa
    21541 atgccagtta tgtaaataca gtggcaaatg aatgggcaca aaagaaaatc gccacagcag
    21601 aagaagcgat ccagcacatt cggcaaataa gtaatcaagc aaaacaatca aaacaaaaga
    21661 agcaaacaaa ttatcaaact ggcaagcgaa atgttcgacg ggaaaagctt cctgattggg
    21721 tcaaccagcc aaaagatgaa aaacagatca gcagcgagaa aaaagcagag attgaccgcc
    21781 gttttaaaga atacctggca aaaaaggagg gtgatatcta gtggaagacg taggcaaaga
    21841 attgaagagg atcatcagca aacgcaatta tcaagaaaag tatgctgaaa tgatcgctga
    21901 agtcttaaat gatgcggatg ttcaagcttt tctaagcgca caccaagaag aattgacaca
    21961 agcggatatt gaaaaaagtt acgctaaatt gtatgaattc gtgcaagaaa agcgtaaata
    22021 tcaaaaaaac gatccaacga tgatcgctcc cggctatgag ccgaagctga cgttgaattt
    22081 tcattatgtg gatgtcactt atgtaccaac ggaagcattg ttagcacggc aaagacaaga
    22141 agaaatcaga agccgagtaa aagcaatgga tatgccaaaa gatattcaat cggcttcttt
    22201 tgaaaacttt gaacgtacag atggacgagg atatgcgagc ttagaagccc tagattttgt
    22261 agagaaatat actgctgatc caaaagggta tcataaagga ctgtatctag taggaagttt
    22321 tggtattgga aaaacatatt tattaggagc aacagcgaga gaattagcga ttgctggatt
    22381 caccacgacg ctagtccatt ttccgacatt tgccgttgag atgaaacagg caatcggaaa
    22441 agatcaagtg gcagaaaagt tagatgcagt gaaacgttca cctattttga tgatcgatga
    22501 cattggtgca gatgcgatga gtagttggat acgagacgaa gtattcggtg ttatccttca
    22561 atatcggatg caggaacagt taccaacctt cttctcatcg aattttacaa tggatgaatt
    22621 ggaacagcat ttgtccgtca ctcaacgtgg agatgaagag ccgctaaaag cgaaacggat
    22681 aatggaacgc atacgttatt tgacaaaaga aatcgaaatg actggtcgca accgacgcaa
    22741 tggttgaaca gttgacaagt ccaactgaca tttgacctag tagtaaaagc aggtcagcga
    22801 atcattatat ggacagaaat gaccggagac aatcaccgca acaagtaatg cagaaactta
    22861 aacggaggat tttttatgag ggctattttt tctagtatat ctgtagttgg tctatttttt
    22921 cttttagcag gctgcggtag cgcagatagc aacacaggtt ctgttgattc gattgatgcg
    22981 ggtcaaacag taaaaacaag cagtacgaaa acggagagac aaagcatatt tgtcggaacg
    23041 attgaaagta tcactcaagt agaagcagaa gaagaaacgc ttcagttgaa actcgtcgat
    23101 gtcatggcca aagaagatcc agcgaatatg ggaacgtcat tcagcaatga cggagtgatc
    23161 ttaaatgcta ctgctgaaaa aataagcgtg gacataaatg aattgaaaat cggtgacaaa
    23221 gtccaattta cattagttga gatgccgatc atgacgatgt ctatcccacc gcaagttcct
    23281 ggagattcga ttattgatgt ttcattgaac aaataaccaa taacattttt tcaaaaataa
    23341 gaaaatgttg ttttatagaa cgaaaaggta tattttttaa ggagatatgg gaattt
//
LOCUS       DAHHZJ010000028        23328 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.029, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000028 DAHHZJ010000000
VERSION     DAHHZJ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.029"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(10..2154)
                     /locus_tag="NBL02_000893"
     CDS             complement(10..2154)
                     /locus_tag="NBL02_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCD2571598.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(2511..3884)
                     /locus_tag="NBL02_000894"
     CDS             complement(2511..3884)
                     /locus_tag="NBL02_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCD2571599.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(3884..4279)
                     /gene="rbsD"
                     /locus_tag="NBL02_000895"
     CDS             complement(3884..4279)
                     /gene="rbsD"
                     /locus_tag="NBL02_000895"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCD2571600.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(4307..5224)
                     /gene="rbsK"
                     /locus_tag="NBL02_000896"
     CDS             complement(4307..5224)
                     /gene="rbsK"
                     /locus_tag="NBL02_000896"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCD2571601.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(5332..6345)
                     /locus_tag="NBL02_000897"
     CDS             complement(5332..6345)
                     /locus_tag="NBL02_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCD2571602.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(6426..7025)
                     /locus_tag="NBL02_000898"
     CDS             complement(6426..7025)
                     /locus_tag="NBL02_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCD2571603.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            7211..7912
                     /locus_tag="NBL02_000899"
     CDS             7211..7912
                     /locus_tag="NBL02_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2571604.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            7922..10633
                     /locus_tag="NBL02_000900"
     CDS             7922..10633
                     /locus_tag="NBL02_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2571605.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGTIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            11084..12712
                     /gene="treC"
                     /locus_tag="NBL02_000901"
     CDS             11084..12712
                     /gene="treC"
                     /locus_tag="NBL02_000901"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCD2571606.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12772..13953)
                     /locus_tag="NBL02_000902"
     CDS             complement(12772..13953)
                     /locus_tag="NBL02_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCD2571607.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            14205..15728
                     /locus_tag="NBL02_000903"
     CDS             14205..15728
                     /locus_tag="NBL02_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HCD2571608.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(15797..16525)
                     /locus_tag="NBL02_000904"
     CDS             complement(15797..16525)
                     /locus_tag="NBL02_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD2571609.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(16538..17818)
                     /locus_tag="NBL02_000905"
     CDS             complement(16538..17818)
                     /locus_tag="NBL02_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCD2571610.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(17959..18561)
                     /locus_tag="NBL02_000906"
     CDS             complement(17959..18561)
                     /locus_tag="NBL02_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HCD2571611.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            18806..19012
                     /locus_tag="NBL02_000907"
     CDS             18806..19012
                     /locus_tag="NBL02_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571612.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            19324..20043
                     /locus_tag="NBL02_000908"
     CDS             19324..20043
                     /locus_tag="NBL02_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCD2571613.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(20124..20807)
                     /locus_tag="NBL02_000909"
     CDS             complement(20124..20807)
                     /locus_tag="NBL02_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HCD2571614.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            21025..23211
                     /locus_tag="NBL02_000910"
     CDS             21025..23211
                     /locus_tag="NBL02_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCD2571615.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         7765 a         4200 c         4571 g         6792 t
ORIGIN      
        1 taatttattc tatttcttta aaggcagatc aagccagaat gatttgtcta aacttaacaa
       61 ggttagtaca ggaacagttg cagctttaac tgtacgagta gttgtttctg gtatctgtct
      121 tgccgtaaaa ttgttccaca aatcccagat tgaagcgaca cggaagatgg aagaagcgtt
      181 gttgacgcgt tttatcgttg aaaattctaa tcctgtgatt ggcacgccat ctgttgtcca
      241 tttgattggc acatcgaatc gcttagacat attgaggatt tcagcgtttg cttcttcagt
      301 tgttgctgct tcttttttcg gttcatcata ttttgtcagg tcgtacgctt cgatgatcgc
      361 gttcaatttt tgtgaatatt ggcgatctgt agcataacgt ccttccaaat atttggttgc
      421 ttctttgtag tcttttgttt ccgacttcca agttggtttg tagaaatcag gattgccagt
      481 cagtccattt ttgatcaagt cggcatagtc ttccaaactt tccttgtaag aaggatattt
      541 acggaattgt gcgcggattt ggtagctttg tccggaacta tcttgttcaa gtgtattgaa
      601 ggtcacgctg cttcctttat atgatccttt gataccaaaa aggttatgat ttggttcgct
      661 tgataataag ctgtttccag atccactttc aaggatagct tgagccatca tgactgatgc
      721 gtagattcct tctttttgcc cgatttcctg tgcatcatct gcaatctttt tcaagaactg
      781 atcagttgtt tggttttgac tgaaatggaa cgagccatct aatccatcta aaatagatga
      841 gccgcctgtc agcgaacctg aagcagcagg taaacctgct aagtttactc ggacagcaaa
      901 atcaggcgtg aaatctgaaa tagaagttac tcgaacttta tctcctggtt gaggcgcatg
      961 gataaattgt ccgttcccaa tatagatacc tacatggtga gtaacaccgg aagaaggtcc
     1021 ccagaagacg aggtcgccag gttgggcctg acttactgga atctgcgtac ctgcatattg
     1081 ctgttcgcct gtccagccac cgatataatg accggttgct ttcatatata cgtaataagt
     1141 caaaccagaa cagtcaaaag tatttggacc ttttgctccc catacatatg gttttccaag
     1201 atgcttgaat gcttctcgaa cgattgcttg ttggacattc gttgtatttg taggcaatac
     1261 agcatcaatt ggtgatttta caacaaaatc agaagaagca gaagatggat tctctatacc
     1321 gctttgattt gctgtatttg gacgagatga agcattgttc gttccgccat tcgatgaacc
     1381 attgtttgga tggtttggtt ctctctgagt atttccattg ttattcgatc cagatgggtt
     1441 tgtttgattc gaagaattcc cgctgttcga attattgtta ttattgtttg aattatttga
     1501 accattatta ttgttgttat tacccacatc tggtttgttt ggcgtttccg gatctggtgt
     1561 actagggact tcttcactag gatcagttgg tttctctgtt gaagtatccg gtccagcttt
     1621 aaattctgca gtaatagata aattgttcgt acctactgta aaggtctttg catcataatt
     1681 tacttctttg gcaccattaa cagcgactga agcaatttgt tcaccttcag ctggcgtaat
     1741 agtgtaagtt accttcgttt ccgctaataa accttctacc gttccacttt ccgtaatatc
     1801 caaaggttgc tgttcagggg aatccgctgt cgaagcagtc agttggatct taccaatcaa
     1861 agaaggagca attgtaaggg aatatgtctc ggctggcttt tcattttctg atggatcagc
     1921 cggatcaatt tcagttggct cggtcgatcc tgttgttgag ttagttgttc cagttgggtc
     1981 ttctgtaaaa gaatccgttg tttcactagt agctgagctg gatgtagtcg tatctgttgt
     2041 gactgtttga tcagctattt gattatctac ggcaaatacg gtattggtcg acaaaacaga
     2101 cggagcaatc agccaagtag cagctagcaa acgtatcgtt tttttatttt tcataataaa
     2161 ctagtccttt tcctttcatt gattttctgt cttattcagc attcattaga cattttttgg
     2221 aacagataat cgtgtcgaaa agctttttct tttcataaat ctgtctgtgt attctaaata
     2281 aaacaatttg taaataagca acgagcattc attcgttttt ctttgacgtt cattttattg
     2341 catagaccac ctttgtttgg ggataaacaa gtggggataa aaatggttat gcatatgtaa
     2401 attataccaa agtttggaaa gtatcacaaa aatttataaa aataatgatc ctaaaaagaa
     2461 aaagactgaa atatgtgata aacatctcat acttcagtct ttttcattaa ctattcataa
     2521 gacattaggt cttcatcaaa cccgctaacg agagtagtga tcattgctac gactagacta
     2581 acagccagtc ctgctaagta ccagaaaaaa gtaccggaaa tgaatgcagg caatgtggtc
     2641 agactaccaa atacaaaagc gatcgctttc acttgggcaa atcccattac agctccacca
     2701 gcagctgcac caatgcaagc agcaacaaat ggttttttca atgggatcgc aattccgtaa
     2761 acagttggtt cggtcactcc cgcaagtaaa atcgaagcaa aagcagaacc ggagatcgaa
     2821 cgggtttttg aacgtttact tcgcagaaat acacctaaag ctgcaccagc cattccaaaa
     2881 ttggctgctc ctgtcaacgg aacgattcgg tcgaacccat aagcagagat attactgatc
     2941 atgacagggt ttacagccca ttggatacca aatattacca aaatattcca accaccagcg
     3001 atgattgcgc cactgatcgg tccgttaata tccatcatcc aagtaattgc ggcagctaaa
     3061 ccttcgccaa ggtaaacacc cgcaggcccg aataccatta acgtcaaagg aaccgtgata
     3121 actaatgaaa gtaaaggtac aaatactaat tgcatatttt cgcgaatata ttttttgagg
     3181 aatttttcaa caaaagaata aaagaaaata cctaaaatcg ccggcatgac agtagacgta
     3241 tactgcatca gtactacagg aatccccaga aaatcagtag ctgctttgcc actgtctgaa
     3301 agtaaagagg tcagattcgg ttcaatcagt gctgcagcaa tgacagccgc aatatatcca
     3361 ttcactttaa acgttttggc agcagaaaaa gcaagaaaga ttggcagaaa ataaaagacg
     3421 gcattcgacg cagcagacaa aatatgataa gttccgcttg tttcactaag ccagccaatc
     3481 gtagtcgcta aaacaaccag accttttaat ataccagaac cggcaaacag cggaatgatc
     3541 ggcgtaaaga tttccgatat caaaccaaat agtttttcga tcggatttag cttttctttc
     3601 ggagctgaat gatcgatttg acgcgtcaat tccttgtaag caggagccaa tccctgatcc
     3661 atgattattt ctgcatatct atcgagtaaa atgaaatact taatggaagg gatttgctgt
     3721 tttaattttt ctgcattggc tactggctgt tttttcaatt tgatacgaag ccgagtcgca
     3781 catggaagga cagaatcaat attgtctttt cctcccaact gttcaatcag catatgaatc
     3841 tgctcggaca gtaccatctg ttcaggattc attgtcgttt ccattagaag accactccac
     3901 tcactaatat aatattggcg taaggtgtca tttcgcctgt acgaataaat gcatgacagt
     3961 tattcaactc ttttttcatt tcttcatgtg ggataaagct aatcggtgta tctggtaaca
     4021 ataatttgat tttttccaat atttccgggt tcattgtctt tatttcttcc gccaattcaa
     4081 tcgattgtac ctcaagttct tctaaaacgt tttctaaaac agataaaaaa cttggaagac
     4141 cgtttgagac agccaaatct attttttcag taccaaaggg gacaggcatg cctgcatccc
     4201 cgatacttaa tttatcaaag tgccccattt gtgcgattac tcgtgaaata tctgaattga
     4261 ttacttttcc ttttttcatg tttgtttctt cctctcattg ttaattttag actgctgatt
     4321 gttctaaagc ttcgcggtaa gggatagaag gttgcgcacc ataagactgc acagcaacag
     4381 aagaggctag atttccataa aggatcgctt ctttcaagtt actaaggtct ggctgaagtg
     4441 tgctgcttaa tgcgccaata aatgtatcac ctgcggctgt tgtgtcaacg gcatccactt
     4501 taaaagcagg gacgataccg tgttcttttt ctgtatgata gaaagcgccg gcactgccta
     4561 gtgtaatgat tactgttcca atacctaatt catggagttt ttcggctgct gcgaccaaac
     4621 tgttatgatc tgtcactctg attcctgtaa tgatttctgt ttctgtttca tttggtataa
     4681 tgatatctgt cttttcaagt agttcagtag gaatcgtttc acgagctggt gcaggattca
     4741 agatcgttgt ttttcctgcg tcttttgcaa tagaaaaagc taggatcgtc gcatctaagc
     4801 tactttcaaa ctgagcaata ataaagtcac tgtttgtaat cagttgcttg ttttttagga
     4861 cttcttgttc atggaaagca ttatttgccc ctgcatggat caagatacta ttttctcctg
     4921 ctgcgtctac cataatcatt gcttgtccgg ttgtggcgcc ttggatttca gctatcccag
     4981 atgtatcgat gttttctttt gtcagtagat ctaataactg ttgtccttcg ctgtcagccc
     5041 caacgcctcc aataaaagat gtccgtgcgc ctgaacgttt ggctgccact gcttggttgg
     5101 cacctttacc tccgccagcg tgaaaaatct catgcgcatg catcgtttcg cctggcttgg
     5161 gcatgttggt taaacgaagg gtagtgtcca tattaatact tccaattact gtaaccgtgt
     5221 tcattttaca aacctccaac tatctcattt attttgatgg gaaaattatt ttttaaaacg
     5281 ttttagtaaa acgttttaat agttcgataa aaatatagca caccttgaaa gctatttcaa
     5341 gggtgcagtt gaaaatcttt tttctaatcg tacaggtaat cttttttctt cccattcttt
     5401 ttctgggaat tggatccgat ctaacaataa cttggcagat gtttgtccca attcgaagat
     5461 tggttgtgcg atcgttgtca gtttcggctt gatatactcg cacatatcta tattatcata
     5521 gccaataatg ctgtagtctt cagggatgct ttttccagct tcctctaatc cgcgatataa
     5581 tccaaaagct aattcatcat ttaaagcaaa cacaccagtt gcagcagaat caagaagttc
     5641 tgctgtgatc tggtagccac cttgttttga gaactgtgta ggtaagagga tcagttggtc
     5701 gtgagggatt tggtaaacat caagagcaga tttaaacccc tcaatccgag cgtggacatt
     5761 ttccggtgga ttttcaggat agacaagggc gatcgtttga tgtcctagac taagcaagtg
     5821 catgcccgct aggtaaccac cgcgaaaatc gtctgttcgt acggcatcac taaatccttc
     5881 agattttttc tgatccaaaa caatgaaggg ccggccttgt tttttcaaat tttcgttgat
     5941 tgcatctgta gagacagcag aagtcgcgat aatgaatcca tccacacctc gacgtgtgag
     6001 ctcggctaga tattcgattt ctttttgatg atcagaatca gcattacata atatcgtgac
     6061 gaaattctgc ttatacaaga tatcctcaat tcctcgcatc agtgtactga agaatggatt
     6121 cgtgatatca ggaacgagta caccaattgt tttcgtttcc ttggtaatca tttgtctagc
     6181 aaaatagtca ggctgatagc ctaattcttc tttggcagct aaaactttct gaacagtttt
     6241 cggactgaat ttctccgtat ttccatttaa tatcaaggag acagttgtga ttgaaacgcc
     6301 ggaaagttta gcgacttctt tgattgtgat cttttttttc accatgttca tactcctatc
     6361 taaatcctgt ggttattttc ccatgaaatc aaaaagaagt aaatgaaaat cacgtgtgcc
     6421 agtacttatt tgctttgttg gatagcttcg attatttgtt tcgttgagcg tactcgtgca
     6481 gttttcggaa atatcttttt tatgaacaat tgatgggatt ctttgtcacg atcactgcaa
     6541 gcatcttcag caagcacgat atggtacccg tgttgaaagg catctaatgc agtagcatat
     6601 acaccgtttg ttgtcgctac accgcttaaa atgatagttt cgattccacg ccggcgaagt
     6661 tgaagatcaa gagaagtacc aaaaaaagca cttggattgt attttgtgat agtcaacata
     6721 ttatctgtat ctttcaaaga atccttcaaa agtaaatcag tgaattctgg aggtacagaa
     6781 gcagtcattc cacccatatc agtttgttga ctcaaataag ggaaagtgga gatgtctacg
     6841 ttcactaaaa ctttcaaggc agcggtacgt ttcaatgttc ttactaacaa atcattgttt
     6901 gtcagaatag atgctgcagt gtgtggagag agtgtaccgt tatttacgat ctctttttgc
     6961 atgtcgatcg cgacaaaagc agtttttgat aaatcaaaaa tcatattttc ctttaaatca
     7021 ttcatgatgt tccttctttc aaataacaat gttttttatg taagtaaatc ataaatcgtt
     7081 tatttgtcaa ctaaattaaa ggattccttt ctttagtcta gagaaattca gtggtaaaat
     7141 aaataagtgt gagaaaacgc attcgatgca tagatatagc gagaaatatc gtgaaaatgg
     7201 aggaatcaga gtgaagtatg tcgaagtaaa aaatgaatac aaacgataca aaatgggaga
     7261 aacgaccatt acagcaaatg acgatatttc tttttcaatt gaaaaaggcg aattagtgat
     7321 tatacttggt ccaagtggag caggaaagtc aacgattctt aatattttag gtgggatgga
     7381 tacacctgat gaaggtcaga tcattattga tgacacagat attgcgcaat tttcagataa
     7441 acagcttact gcttaccgac gaacagatgt agggttcgtt ttccaattct acaatctcgt
     7501 tcctaatcta acagcaaaag aaaatgtgga attggcaacg gaagtttctc ctgatgcgct
     7561 tgatcctgta gaggtattga ggcaagtggg cctagctcat cgattgaata actttccgtc
     7621 ccaattatca ggaggcgaac agcagagggt ttcaattgct cgtgcactag ctaaaaatcc
     7681 caaacttctt ttatgtgatg aaccgacagg cgcgctggat tttgaaacgg gcaagcaagt
     7741 attgaaatta ttgcaaaacg ctagcagaca acacggcaat accgttttga tcattacaca
     7801 taattctgct ttagcgccca tcgctgatag agtcattcac attaatgatg ctaaagtaag
     7861 gtctgttgaa ttaaatgatc atccttcatc cattgatgag attgtatggt agggggattt
     7921 tatgaaaaca agaacatatc ttaaagcagg ttttcgcgaa attcggcaat ctaaaggacg
     7981 attcattgca atcatcctta ttgtcatgct aggaacactt ttatttgtag gtgtcaaaac
     8041 tgctggacct gtcatgcaaa aaacgatgga tcactatgtt ggtacagcag gactttctga
     8101 tttgcaaatc gtttctactg gtggcttgac acaaaaagat attgctcaag cagaaaagat
     8161 cccagatgca aaagtagaaa caggaaaaca gatctattat tccaatactg gcaaaaacga
     8221 agtgattcaa atattttctt ataacaaaaa taacaagcag aacaagctac aattaactga
     8281 tggaaaactc ccggaaaaac caaaccagct tgtattggat gaaaaagcgg aagatgaagg
     8341 gtataaaatc ggtgatattt atcagattga ttcagatgaa ttgaaagaaa aagagtatac
     8401 catcacgggc tttgtccgtt ctccattgtt catcaacaat ttggagagag gatacgcgaa
     8461 cgtcggaaat ggtagcgtcg attattttgt ctatttacct gaatccgttt ttaaaacaga
     8521 tatttattct gtcttatatc ttgattttac aaacgtaaag aatttgaata cgtacagcaa
     8581 agcttacaaa gataaaatgg agcaaaatca agaaaaggca gaaaagtatt tacaagatcg
     8641 acccgaagaa cgcttagaag agctgaaaaa atcggccaac gaagaattag aaccggcaca
     8701 gcaaaaaata gcggatggca aagcacaaac aacgcaagca agaacacggt tagaagatgc
     8761 taaaaaacag ttagcgcaac agtcagcaac tatccagcag cttcctgaag cgcaacgaac
     8821 aatcgctgtt caaacattat ctcaacaaga agctcaactt gctgaacaag aaaggcaatt
     8881 agttgagaaa gaacgtgaac tggcctctgc agaggaagaa ttgaatcgaa atgcagaaaa
     8941 aattgaaaat ttaactaaac catcatacct atacaatgaa cgttcagaaa atgtcggttt
     9001 tcaagaattt ggtgatctgt ctgatcgtat cgctgcgatt gccaatgttt ttcctgtttt
     9061 ctttttcttt attgccgcat tgatcacctt tacgacaatg acacgtatgg tagaagaaaa
     9121 ccgcagggaa atcgggacat taaaggcgtt aggttatacg aaagttgaaa tcgcaaaaaa
     9181 atatgcaatc tatgcaagtc tagcctcagg gataggtatt gttttaggaa cgatattagg
     9241 aacgaatctt ttgccaagga tcatttttga gctgtctaac gagcggtatg atattgggag
     9301 tgcgcttatt ttttatgact ggccgccgat tttacaagca gctgccgcat tcttcattgc
     9361 cgcatttggc gctgcaatga tcgttttatt caaagattta agagaacggc ctgctgcatt
     9421 gcttcaaccg aaagcaccga agccagggaa acgcattttt cttgaataca tcactccgct
     9481 gtggtcacgc cttagcttta atcaaaaaat cagttaccga aatctcttcc gatataagtc
     9541 aagaatgctt atggcgatta tcggaattgc tggatgtgcc gggctgatgg ttgcaggcgt
     9601 gggattgaaa gattctctaa gttctgtttc agccaaacaa tttggaacga tcattgacta
     9661 tcaagcaatc gttacattgg attcatccac ggaagatagt cgtcaaaaag cagcggatat
     9721 gctgcagaag caagaaaaag taaatgagat cctagctatt catactcaaa ccgtagaatt
     9781 gagaaaaaaa ggacatgctt cgcaaagtct gacagtgatc gtaccaagta aaacagctga
     9841 tttggatcgt tttatccatt taactgatct tgaagagaac ccacgctcct tgcctaaaac
     9901 aggaatcgct attacgcaaa aagtagcaga actatttgac ttatctacag gagatcatat
     9961 ttcgctttat gatgaagatc aaaaagaatg gaaagtcgaa gtaaaagaaa tcgtgaaaaa
    10021 ctatctaggt agctttattt atatgtcccc tgaatattat gcttcagtta ctgatcaatc
    10081 gatgatggat aatgcatttt tgctgcgtac agataagatg acaacaaaag aagaagaaat
    10141 gctttccgag aaactgcttg ccacagacgc tgtgacaaat acgtctttca tgtccaaaca
    10201 aatcgaaacg caagaagaat caatggcgaa cttagactcg gttgtcttaa tctttgtcgt
    10261 attatcagga ctgctggctt ttatcgtctt gtataatcta actaatatca atatttccga
    10321 gcgtgtacgc gaattatcta caataaaagt ccttggattt tttgataaag aagtcacgat
    10381 gtacattgtt cgagaaaata tcatctttac tcttttagga atcatcggag gatttggtgt
    10441 aggatatata ttgactgact tcatcttgca acaagcgtct atggagaatg tcatctttcc
    10501 attagtgatc acatggtggg cttatgtctt atcagcagga ttaactatcg tatttacggt
    10561 tattgtgatg attgttaccc atttcaaatt aaagcatatc gatatgattg atgcgttgaa
    10621 atcgaatgaa taaaaacaga ggttgtgaaa tcagcggttt gacctgagta ttagagaaac
    10681 aaacggcaaa atagtttttc atattttgcc gttttttgag ctaataccga aggacagctg
    10741 attgaacacc atgaatcaga ggttgtaaaa aagccgtcct gcatcgagca aaaagaatac
    10801 tcaaataaaa ttagctcctc atgttttaca tttgagaaga ctgattgccg tagataaaag
    10861 cttttgaaca ccatttatag aagaaacaag aatattttgc tattttaaac aagaggatag
    10921 acaaatacat gtctgatctc ttgtttttta ttgggcaaca gacctcttag aatgtataaa
    10981 gataaatttt acaaacgctt tcggttcagt tataataata aatgataact tgtatatata
    11041 agttgtggta ggtaataaat cagttgaatg gaggtaaata tccatgacat ttaaagacaa
    11101 agtcatttac caaatctatc ccaaatctta caaagacagt aatggtgatg gaattggaga
    11161 tttaaaagga atcaaagaaa aactcccgta tctgaatgat ctaggcatcg acatgatttg
    11221 gctaaaccct atttatccaa gccctcaaaa agacaacggt tatgatatct ctgattacac
    11281 agccatcgat cctatctttg gtacgatgga ggaattcgaa cagctaatag ccgaagcaaa
    11341 gaaataccgg atagaaatca tgctcgatat ggtgctgaat cacgtttcga cagaacatga
    11401 atggttcaaa aaagcactag caggggacca gcattatcag gacttcttca ttttaagaga
    11461 agagccaacg gattgggtat caaaatttgg tggaagtgct tggtcgccat ttggagatac
    11521 ggggaaatat tatttgcact tatatgatcg gacacaagct gacttgaact ggcggaacca
    11581 agcagtaaga gaggaactat tcaaagttgt tcgtttttgg atggaaaaag gggtaaaagg
    11641 attccgtttc gatgttatca atgttatcgg taaagacgaa caattgaaaa ataataaaga
    11701 aaatgatggc aaacctgaat atacggacaa accaatcacc catacgtatc taaaagaatt
    11761 gaaccaagca acgtttggga cggattccac tatcatcaca gtcggtgaaa tgagctctac
    11821 gacgatcgaa aattgtatct tgtatacaga acctagtaga gaagaattat caatggtctt
    11881 taatttccat catttgaaag tcgattatga aaatgggaat aaatggtcta ctcctccatt
    11941 tgattttgca gaattaaagc gactatttca tacatgggga gaaaagatga gtgagggtaa
    12001 cggctggaat gctctatttt ttaataatca tgatcagcca cgtgcgctca atcgatttgt
    12061 agctatcgat cagtatcgaa aacaaggagc agagatgcta gcaaccatga tccatttgaa
    12121 ccgtgggacg ccctatattt atatgggaga agaaatcggc atgatcgacc cagactattc
    12181 atcgatttcc gactatattg acgtagaaag tttgaatgct tataagttac tccttgaaca
    12241 aggattcaca gaaaaagaag cgtttcgtcg gatcaaagca aaatctcgcg ataactctcg
    12301 aacacccatg caatggtcag acacagaaca agcaggattt actacaggga aaccttggtt
    12361 aaaagttgga ggcaagctag aagagattaa tgtagaaaaa gaactatctt ctgaagattc
    12421 tattttgtcc tattacaaga aattgattcg gctgagaaaa acatatccaa tcgttgcaca
    12481 aggagattat catgcttatg aagcaaatca tccggaagtt tatggctttc ttcgccaatt
    12541 tgaaggacag cagctgcttg tattaacaaa tttctatgat aaagatacaa ggatcgccat
    12601 cccagaagga ttcacagaag ccaatattct tatcgataat tattccgata ctgttcttca
    12661 tcaaagtctg gagctaaaac cataccaggc aattgctttg ctgaaaaatt aaaacaaaaa
    12721 cacaaaacgt cgttcctacg agctattctc gtaagatcga cgttttgtgt tttatttaaa
    12781 ataatcttct tcagaaaatg aagagttttc atactctcct ttatcgccat aaaatttatt
    12841 gtagcgttgt ttgaatttac ggaaaataaa agtaacgagt aatttgacta acgtatagat
    12901 aggaatacca aaaataacgc ccacaattcc cattagatca cccatgacaa gtaaaacgat
    12961 taagatcgta atcggatgga tctgtaaacg atcacccatt actcgaggaa tcaccaagtc
    13021 accgtggaga agttgtacgg caaaccagac aatgacaaat ttgacagcca taaaagtaga
    13081 gtcttggaat gcaatgatca aacctggaaa gaaggcgata aacggcccaa tatatggaat
    13141 gatacataac aacccagcag aaatagctaa aacactagca aattctaatc cgatcagtaa
    13201 gaaacaaacc cagtagactg ctccaaggat aaatgaagca atgatttgcc cctttagaaa
    13261 tgaacccaat tgttgattag caatctttgt caaattttta aaatctgtac gaaatgctgg
    13321 tgggacaatc gctaaaactg actgataaaa tttttgagga tctctcaaaa ggaaaaaagc
    13381 gacaattgga ccagtaaata aagtaataaa aatagctgta acagttgaaa aaatgttgct
    13441 gatccgttgt gcgccacttt cccaataatt tccaataaat ccagctatat cagacgtcac
    13501 gtcgtcaata gaagcgaaaa attgattaaa tgaatcggca aaaggtgttt tctccaaaaa
    13561 ttgtcggaga ctctcttgaa aatcttgaaa caaatctggc aaactactga tcaatgtctt
    13621 tgcctgttct tcaagaaaag gaataagcca tatgcttcca agccccaata aaaataaaat
    13681 aatcacataa acgataatga ctgacaagtt cctcgaaagg tgcttgctca aacgtttgat
    13741 gactggttcc aaaagataat acaagatcag tgcaaacaat aatgggggca agatagtagc
    13801 gataatgatt ccgacaggct tgaatataaa cgagatctga tggaaaaaat aaatgatcag
    13861 cgcaagtaag ataactattc ctaaaacata gtaagaggtt tttcctccta aaaagcggat
    13921 caaacgattc gttgtatctg tttctttttt cactcttttt cctcctttat ctataaattc
    13981 attatagaca aaagcgggta taaaaggaaa atgatagagg ttgattgcaa aaatatcgtc
    14041 aatcattcat ataaactttc tcatcaatta aaataagaaa acgcttgaca gttcttttaa
    14101 ccatatgata aattaaatcc tgttgctaaa acgtaactat tacggtttgc gaaaagcgaa
    14161 tcggaacatt caaatgaaag aagatgagaa gaggagtgtc ataaatgaaa cgattgatag
    14221 gtatattggc catgctggtt ttagcaggta tgttaactgc ttgcggggca agtgggaaag
    14281 ccgaggacag taaagaaaaa ttgagcgtga tgacgacctt ttatcccatg tatgatttta
    14341 caaaagcaat cgtcggggat gaaggagaag tagagttatt gattccagca gggactgatt
    14401 cccacgatta tgaaccttct gctaaagaca tggcaaagat ccaagataca gatatctttg
    14461 tttataatga tgaaaacatg gaaacatggg tccccgcaat ccaaaaaaca ttacaagaag
    14521 gcaatgtcca tacgataaaa gcgacagaag gtatgctttt acttccggga agtgaagaag
    14581 gacatgatca tgaccatgaa cacggggaag aaggccatac acatgagcta gacccacatg
    14641 tatggctagc tccaagttta gcaatcaagc aggtagcaaa catacgtgat cagctgattg
    14701 aagcttaccc agaaaaacag gaagtatgga caaaaaatgc agcagcctat accgagaaac
    14761 ttcaagcact ccatcagctt tatcaagaaa cctttaagca agcaaaacaa agatcttttg
    14821 tcacgcaaca tgctgcattc aactatctag cgttagaata tggattgaat caagtatcga
    14881 ttgctggtct ttcttctagt gaagaaccgt ctgcggcgag aattgctgaa ctgaaacact
    14941 ttgttaaaga acatggaatc aactatattt attttgaaga aaacgccaaa gatagtattg
    15001 ccagaacgtt agcaaacgaa gcaggtgtat ctttagaagt attgaatcct ttggaaggat
    15061 tgacgaatga acaaatagaa aatggagaaa actatctttc tatcatggaa gcaaacctcg
    15121 aagcgttgaa aaaaacgaca gagacagaaa acccgctaga agatactttg acccccaaaa
    15181 aagaaaagag cgtctacaac ggttattttg aagacgagga tgtacaagat cgctctcttt
    15241 cagattgggc aggaacttgg caaacagtag atacgtatgt agaagatggg acttttgatc
    15301 ctgtttttga atataaagaa aaattgaatc aggataagac agcacaagaa tataaagaat
    15361 attatacgaa gggctatcaa acagatattg cttcaatcaa gatcagtgag aaagagatgg
    15421 catttacgtt taagaatgga acaacgaaac gttcaaatta tcgttatgta gggaaagaaa
    15481 tcttgaccta cgaggcagga aaccgtggcg taaggtatct attcgaagca gaagataagg
    15541 aaagtggcgc ttttagatat attcaattca gtgatcacac gattgcaccg gctgactccg
    15601 aacattttca tttatatgca ggaaatgaaa gtcaagaagt attgctgaaa gaaatggaga
    15661 attggccaac attttatcca gatacaatgg atggacgtga aattgcacag gaaatgcttt
    15721 tgcactgata ggcaaaaaag aaccgaaggg tttggatcgg ctggtcattt agtcggtcca
    15781 aactattcgg ttctttttat gaaatcaatt tctttaatcc acggatagag gctgggatgg
    15841 tctcgccgtt tgccaacgtg acttctctgg tttttttatt aatcgaccgc acattttgtg
    15901 tattgataac ataggattta tgacaacgga taaaattccg atgcattttc tcgatttctt
    15961 tgatcttctc atagaattgt aattgggcat gagtcaaatg gacttccagt ttatgtggcg
    16021 ctcctagcga agtagaaaaa tattggatgt ctttgatcgg taaaaatttg atctcatcat
    16081 taaactctat tttgaagata tctttttgga tttgggacat ttgaaaatct tcgaaaattt
    16141 tgctcaggat atgctcgaca ttttctttta aatcataaag atcttctttg acgatataat
    16201 cagtcggttc gatgttgctt tttaagattt tcatcgacat ttccgtatgg gaagtcacat
    16261 agattatttt tcctaaagga tctaattcac ggatcaattt accgatcgct acaccatcca
    16321 tttcactgtc ttctagttcg atatctaaaa agtaaatacc aaaagaagaa gttctgatct
    16381 tggctgcatt gagaagttct tccggatcag aggtagaaat atggacataa ctgtctaagt
    16441 tttcaatcat gattctattt ttgatcaccg ttgataaaac ttctaattgt tttggatcgt
    16501 cttcacaaat ataaatggga atcatatcat tactccttta ttttatttgt atataaatcg
    16561 tttgagaaaa acgctggttt tcaattgatg tttcaaggaa aagataagga ttggtctttg
    16621 tcaattcatc caagataaac aagccaaggc cttcgttttt tggatttgtt tttgttgaga
    16681 accctttttg tttcaactgg tttaacggta atgtatttgt tgttgtattg atcacacgaa
    16741 tcatcacgga atgattcttc ttgagtatgg tcaattcgat ttttttcttc tttgcagcac
    16801 tagctccttc aatcgcattg tccaagataa taccaagcat acgtacaagg tttaccgtat
    16861 gtttcgtatc cagttcgatt ggctcaggaa aatgaagctg gacgatcaac cctttttcct
    16921 gtgccatcat gagttttaat gacagtaaac tacggatctc aggaactttc atattatcca
    16981 acatacttag attggccgga aaaaggttca tcatctggcg tgtaggtaat atcgtttgat
    17041 cataataaga ccgtaatcct tctaagtcgc cttctgaaat atagctgtga agagaaaaaa
    17101 gcagattttg gtaatcatgt ttgaattctc tcaagtcttg atagtttttt tctagatctt
    17161 tgatataatc ctgttgcatc ctgacttgtt gctctaataa ctgatcagct aatttcttat
    17221 tagagatttt ccaataataa accaagatga tcaggccaac acacagatat agaatgaaaa
    17281 aactcgtatt caaagctaag acatcaggtg tttcaccaga aaaacgggta taaaaaataa
    17341 tgatatagta tgtaaataat atcaatgtcc cgatcgtacc gatcatacgg cggtccacag
    17401 tgtgaaacca agtctctaac gattttttta aacaaaaagc taataaagca gataaaaaag
    17461 cggataagac aatgtgaaaa tactgcaatg aatctcctgg ttcaataacc gaagatttca
    17521 aaacaaaaaa atcgccgatg cttgttaagt gatctcctaa aagcgacgtc aataacccaa
    17581 aaggaagcgc gtaaaccaat tgttgacgcg aagtcgaaaa gaagaaaaaa gtaagcatca
    17641 gcattcctat agcaattaga gcagtaaaaa tccctaaaat cgaatagacc catccaatca
    17701 aaatacaaga aatcagaatg actcccgcaa acagatattt gtatcggatg tgatggtcaa
    17761 tcaataagat acaaattgtg taaatcaaat attgcaacgc tatggaccat aaaaacataa
    17821 agtacagctc ccattgattt ttaccaaatt atagcacaat tggcgtatta tttttttaat
    17881 ttttcaggga ttttgaattc agaactcata ccaaagcagc agccagatac aaaaaagtca
    17941 gataagttag ttaattgttt aatgattgtt tttttcatga aagattcctc caaaattcat
    18001 caagtagttt gtaataggaa gcaacgtagc tatttctaaa acaatcccaa gtagcaaata
    18061 ggtagaaaag taagaagctg agaaaaataa cgtaatgaga gaaaaaatta gaaacatgct
    18121 gatcgccttc tttaataatg ggggacgttt ctcgggttca atgggattga ttgctgtacc
    18181 tcttgatcca tagcgaagca gtaacaaaaa gcagctgatt aacaaaaaga ccatcattgg
    18241 gaaggagata gtcaattgca gatgtttcat caccaaggga acgccccata aataaagtag
    18301 tgtccacagc agacaaacga attcagagtc ggaatgcaat ccataagcat atctgcggat
    18361 gcagatataa gaaagaaagg tgattcctgt ttccgcccat gagcctgtca ccagagaaaa
    18421 gactagaacg accgctagtt ttccaagatt attcaataaa acttctaaga caaattttac
    18481 ttggacatac accatatctt cttccgattt gtctccgctg aataaggctt ctaataattt
    18541 gttcacaact ttatctttca tctcatacgt acctccacca caaattttat gctaaaaaaa
    18601 ataaaaaacg tcttttgttt cataaaaagt ggttttgaca tcaaaaccgt catgatctcc
    18661 taaaaaaatc ttcgttttcc aagaacctta ccaaaaaaag tgacagtttt gatacgaaaa
    18721 gtacttttta tgacaagttt gtgctttttc gagtgattta tgttaaaaaa ttactgacct
    18781 tataaaaggt gatggaggga ttcttatgga aaatgaacaa ttgtttgaag tcggcgaaac
    18841 atatgcattt aaatctgatt tatttgaatc accaataaaa ggaaaaatag aaaaaatcta
    18901 tgaaaatagt gtgatgatac gtgtcatgaa atgcgcagta gaagacaatg ataccgctca
    18961 taatctgcac tggcagttag ttgtacgcaa atcaagtaca atagaagaat aaaaacagat
    19021 aaaatgttga gagcataaag aggttgtgag tctcccgttt agctctgagt tacaaggaat
    19081 aatgttaaaa aacagctttt catgttttat gaatattgcc ttgtgaccga ggtgttggca
    19141 ggagaatact gtttatttga aaaaagggct gcgacaagac ttttgtcaca gcctttattt
    19201 tcatgaatag gagttacttt tggctacttt tagagaagta gcttttaagg agcagataaa
    19261 gtcaaggctt ttatttctat aaatgaagta gtaaaatgaa gaacagattt tattacagga
    19321 aggatgccga taattgtgat cattgttgca ggaacgatcg gtgcagggaa aagcacacta
    19381 actgaaatgc ttgcccaaga tttagaaaca aaaccttttt atgagaacgt ggaagataat
    19441 gaagtattgc cgctatttta ttctaatcca gaaaagtata cttttttatt acagattttc
    19501 tttttgaaca aacgcttttt ggcaatcaaa gatgctttca gccatgatga caacgtacta
    19561 gaccgctcca tctacgaaga tagtatgctg tttcatttga atgcagattt agggcgtgtt
    19621 tctgaagtag aagtaaagca gtatgaaggt cttttagaaa cgatgctgaa ggagttagaa
    19681 gaaatttcgc cacaaaagaa accagatcta ctggtttata ttcgtgtatc ctttgaaact
    19741 atgttagcga gaatcaaaaa aagaggccgg gaatatgaac agctagaaca agatcctgaa
    19801 ctctattcct actataaaga actcaatcga cgatacgaag agtggtatga acagtttgat
    19861 atatgtccca aaatagtgat cgatggggac aaatatgatt tcgtagccga tccggcatgt
    19921 ggagaacaaa tcgtacaaga aatcaagatg cgggcaaaga aaatgacaga agaggcagat
    19981 gcccagtcga tccgctacac tacaaataac ccgaaagcaa agggaatctt tacaactaaa
    20041 taatataaaa actaagcaaa ttgctcactc gcagatattt ctctgtgagc agcatcttgc
    20101 ttagtttttt tgattcattg agatcaaata gtgaaccgat tcgtatgata aaattgccat
    20161 aaatgaaatg tctgagtgag ggccatgatc aaaaataaaa tggaaacgat caaaatcgaa
    20221 tgggcgccaa acagatgatg acagaaaaca aggacaatag ctcctgcgat aaaggcaaga
    20281 acgacactta gatagtggaa aaatcgatca aaatctgctt tttctttaga aatacaataa
    20341 tcatataagt tcgttgctac cccttttaaa tttccagttg taaataaatt agtgtaggca
    20401 cgcccgttga ttttatcaaa agctacccac tggatcgatg cagtaaaagc aatcaaaatc
    20461 gttactaatg cgggatgctc atggacgggt gtcaaactaa caaagaaaaa aatcactgct
    20521 tcataatata gaacaaacaa gcgccagaaa tgtttttctt tttttctaaa ataatcaatt
    20581 agatactttg ttagaagaat ccctaagaaa aagaaaaggg tagacaatac ttttttacca
    20641 acacttagcc attctccgtc aaatgcttgg atgatcgata gaatgatatt gccagtttga
    20701 gcagaggcaa aagcaccata ttggatataa gtataactgt ccatggctcc tccgataaat
    20761 gtcagcaaaa gtcctattgt acgcccctcg tgaaaggaag tctgcatttt tttcatcacc
    20821 ttttatgaat atttttgtct tccttatctt agcataaacg attgaaaaaa aggaaaaatt
    20881 ttcagaaaac taaaatcata tcgcttccca ttttcatttt tagaaaggtc tgttagtata
    20941 aagaaaaaat agacaacgaa actttaaata accaacttga aataaacaaa ttcaaagaag
    21001 aaagatggga aggaaatcaa tcagttgaat gaaaaagaaa ttctccgatt acaaaaagaa
    21061 ttgaaacgct gggagaacaa gtataaaaag cttcaacaag tctttcggct acaagaagaa
    21121 atcattgcta cctatgaagc ggcttatggc gaagcacccg gacttattga ggaagaagta
    21181 caagtaaaag aaagtgaagg aaacagctct ttctttaaaa tgatggagaa acgttcctat
    21241 aatcgcttca atcaagaaca aaaagcagct atcacatatg atatgaagaa acatcttcga
    21301 atcatagcag gagctgggag cggcaaaacg caaacaatct gtgcaaaagc tgtttatctg
    21361 atgacgcaaa aacaagtaga cgaagaacgc atactaatga tcacttttac aaggaacgct
    21421 gcaaatgaac taaaaaaacg agtagataat ttcagccaga gaaaaaccgc tgtccatatc
    21481 ggaacattcc atagtatctt ttttaggata tacaatgaaa tttgtcgaaa atttcctgaa
    21541 gtagcaatgc aaggaataca aggagacttt tcaaaggata ctgcacaaaa agtgaatgct
    21601 gttttgcaac agctgatacg taaatataat ctttatatat ttgatcaata tggagaaaaa
    21661 acgattgctt ctcggttaga ctattggcag aatatgaacc tttcagtaga agaaatggtt
    21721 cagttagtta aagaaaaata tgattccatt gataagaatg cacgacagcc gatatcagaa
    21781 cgactgtatg gattgctcac ggaactccaa gaacaaaaaa gaagccagca attgctagag
    21841 ttcaatgatg tactccaaaa tctgaaaagc gctttggaga atgaggagat ccagcattat
    21901 atcggccaaa aatatgatta tctattcatt gatgaatttc aagatactaa tccacttcaa
    21961 tgggaaatcg tgaaacggat gacaaaggaa aacaggatca agttgattgt tgttggagat
    22021 gatgatcaga gtatctatgg attcagagga tcagagccag catacatcaa gaactttgaa
    22081 aaagaatatc ctaccaaaac tcttttctta ttaactaatt atcgttctcg ggcagctatt
    22141 gtccaaggag caaatagatt gatcagctat aataaaaatg atcggatacc aaaggcgatg
    22201 attccagcac aaaaagaagc aggtgttatg gaagcctatt tgttttctga tactaaagca
    22261 gaaagtcagt ggctaatcaa acaaattcag acatttattc aaaaaaatgg gaaatataag
    22321 gaatcaatca tattataccg atcagcttca caaacgcagc agcttgtcca atctctgctg
    22381 aaaacgaata ttccttttgt actggaagca gatagtccat atgaaggtat ctttggtatc
    22441 aagtctttcc aacatttcta tcaaaaaatt gttcattggc aaacagcact gaatctgcaa
    22501 aagaaaaatc aggcctacca gcaattgctc cggcagctga tggcagactg ctacctgaaa
    22561 aaaagtgaat gcgatcaata cttttctcct aaatatcaaa atatagccgt tgcagattat
    22621 attttgtcga taagccccaa tttaaaatcg aaatctgcag aaatccgaga gtttgaggaa
    22681 gcgttgcgta aaacagacca gcatcaagaa atctattcgc ttgttcaaag ctatcttcat
    22741 ctgccacgta tagctaagga aatcgaccag agtgatcaag aatggatacg agaagaagta
    22801 aagcagcatg ctactttcag aagactccaa gccttatcgc aagaaaccca agcgacagct
    22861 aaagaattaa aaacccgctt gattgcttac agacaaggaa aacttgatgc actatgtatc
    22921 cagagcattc acaaaagtaa aggtcttagt taccgaaatg tctttttgat tggttgtaac
    22981 gaaggaagtt tgccttataa cggagcgaca gaaaagaaag taatcgactt taatgaaata
    23041 aaagcagaac cagtaacgac gatagaagaa gaacgaagac tattttatgt tgcgatgact
    23101 agagcaagaa atcgtctgta tctatgtgta cctcaaatga aaaattcaag aaaattgaaa
    23161 gtatcgagat ttatcaaaga aaccgggagt cgtgtgaaaa aaggaaagtg atagaagaga
    23221 gggtgtgaca gaagtggtca gtttcaagaa agaagaagga atgtacgaaa attgcttctc
    23281 aaatttttgt gaaatttcag cttattttcg aagaagctat ttctggaa
//
LOCUS       DAHHZJ010000029        23225 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.030, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000029 DAHHZJ010000000
VERSION     DAHHZJ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.030"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            55..411
                     /locus_tag="NBL02_000911"
     CDS             55..411
                     /locus_tag="NBL02_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HCD2571616.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            398..616
                     /locus_tag="NBL02_000912"
     CDS             398..616
                     /locus_tag="NBL02_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571617.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            613..1026
                     /locus_tag="NBL02_000913"
     CDS             613..1026
                     /locus_tag="NBL02_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002393044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571618.1"
                     /translation="MIPKFRAWVKPDILSNHLNGVIAEALPNFIYGNCLVLREDLEED
                     KQFEEIIDFLDIELMQSTGMKDKNGVDIFEGDIVKTLDADLKVTFSTIKFEEGAFCVD
                     YKDLGTEFDFLYFVDSPLEVIGNIYENSELLEEQR"
     gene            1023..1607
                     /locus_tag="NBL02_000914"
     CDS             1023..1607
                     /locus_tag="NBL02_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HCD2571619.1"
                     /translation="MNKQELIDKLSSLVGSIEDFKGISTFHAGKYAGLEHALELIQQL
                     NESQKPVIPQLVAGWIEKSTDNLTKAEKIAYLIKSKDGDSYYFCDWFVRDGILTQEQG
                     EELLAWATRQSYETLLSLYNGYEVEKEPLYEVIIGDLYLIKKFNNRNDFCFDISRSLC
                     TWEKSAYQLTEAEIKEIDERYWPFAVPVEEVVER"
     gene            1604..1903
                     /locus_tag="NBL02_000915"
     CDS             1604..1903
                     /locus_tag="NBL02_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571620.1"
                     /translation="MSDYLTAEQYEYARKTGDWESGKVLTHGQALNDNQKIVLEWLEE
                     YYAKSQWPFPFYVLKHAANDMPTNIFGFYDDFTMKEEFEVLAAFAQWGLKQEEAE"
     gene            1900..2097
                     /locus_tag="NBL02_000916"
     CDS             1900..2097
                     /locus_tag="NBL02_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571621.1"
                     /translation="MNKRQKNKQFKKQLIKNGYPDKKVKLYCSECGNRLSFKNEYHKR
                     HLLCNEFCFMHSVGLGWNDFL"
     gene            2113..2418
                     /locus_tag="NBL02_000917"
     CDS             2113..2418
                     /locus_tag="NBL02_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571622.1"
                     /translation="MNVQVYLKRDLARFPRSWDEYSCPFEAEEESYLMSANMIEITRD
                     DADEFDKKRSWYSVSNPMYTAVIEDYHISDRFILVDLERPKKRIKQYRRCGFSTKKW"
     gene            2442..2738
                     /locus_tag="NBL02_000918"
     CDS             2442..2738
                     /locus_tag="NBL02_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571623.1"
                     /translation="MKLKDGFYSSSHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVARLVDIKLINDK"
     gene            2814..3230
                     /locus_tag="NBL02_000919"
     CDS             2814..3230
                     /locus_tag="NBL02_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HCD2571624.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHVNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
     gene            3491..3904
                     /locus_tag="NBL02_000920"
     CDS             3491..3904
                     /locus_tag="NBL02_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571625.1"
                     /translation="MDKEIKATVKLDLTELKELLNKASDQVEQIKYQGMDRKRLIDII
                     EQQRLELIQKEAVCKEYKKHLEQVIEYHSVEKYRSVVQKNRATSPDPRQDVSISIKTP
                     VFNSVKSEKLESISTALVEKAAEMNSLANEVSRLI"
     gene            complement(3897..4088)
                     /locus_tag="NBL02_000921"
     CDS             complement(3897..4088)
                     /locus_tag="NBL02_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCD2571626.1"
                     /translation="MVKNLYFVIREATNGQYYFVIKSNNNEVVATSETYLTKYSAEQT
                     INSIKNGITKDSQVIDMTK"
     gene            4559..4771
                     /locus_tag="NBL02_000922"
     CDS             4559..4771
                     /locus_tag="NBL02_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571627.1"
                     /translation="MNENQLRELFKTNEANQTMEATFYETQKSLALIAKQAKYFYDQL
                     ILQGFNEGQAMEFMMRTFSANNQQRE"
     gene            4778..5002
                     /locus_tag="NBL02_000923"
     CDS             4778..5002
                     /locus_tag="NBL02_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571628.1"
                     /translation="MRNYWYVSLSNKYPQPNADDPIRVVQSVQIKKKYSIIEMTREAT
                     PKEIDKYNLRYCGHGYFSEQNIQTNIERYC"
     gene            5029..5562
                     /locus_tag="NBL02_000924"
     CDS             5029..5562
                     /locus_tag="NBL02_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCD2571629.1"
                     /translation="MVNLTPKQKAFADEYIKNGGNATQAAIKAGYSKRSARVIGKENL
                     TKPNIIQYINERLNPIEKKRKLSAEDALNELIDIWQGEVQISVSKQIDRLEKNKVIKH
                     MQYEYTPDLESKLKALDLYLKYKSLLSQTQLEKAQTEIKLMQAKLEQLQINSERSTEE
                     KLDELLEKISGELDGTS"
     gene            5549..6793
                     /locus_tag="NBL02_000925"
     CDS             5549..6793
                     /locus_tag="NBL02_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HCD2571630.1"
                     /translation="MGLVDIYNPKQIDVLNKTIKNDWFITLLHGAKRSGKTKINNDLF
                     LFELRRVRKIADEEDIKEPMYILAGVSSATIQKNILQELYNMYSIEPKFDKHGNFKLF
                     GVKVVQAYTGNIGGVGAIRGMTAYGAYINEASLARQEVFAEIVSRCSGTGARILADTN
                     PDNPEHWLKKEYIDNSSKNIQSFHFELDDNTFLSERYRNNIKESTPSGMFYDRDIKGL
                     WVSAEGVVYRDFDASKHYIQSKDLPPLSNFYCGVDWGYEHWGSIVVIGETDDGTAYLI
                     EEHATQFEEIDYWVDIAKGIQERYGLRVPFYCDSARPEHVARFVREKINAKNAHKARL
                     SGVEEVAKRFKQDKLFICQDRVMKFRDEIYQYIWDKKKGEPIKEYDDVLDSVRYAIYT
                     HELLKKPKVNVNEKIKRVKRMF"
     gene            6806..8302
                     /locus_tag="NBL02_000926"
     CDS             6806..8302
                     /locus_tag="NBL02_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCD2571631.1"
                     /translation="MDKVNEFEYGTDIHYSNDVNTNYVKFSVESNLHYRFSSAEDLLN
                     DSNTLAAMIKHHHEYQVKRLSVLDDYYKARNTNIMDNRRRREKEKADHRSAHNFGKVL
                     CTFDVGYNTGNPIKVQIEDTNQQKEIEEFNTNNDIDGLNAELWLDMDKYGRAYEIIYR
                     DSDDTDYVDLANVFETFVVYDTTVKREPILAVRYPKTRFNKDADKQYIQPIVYTKEKS
                     ITYDETTLTAIELKNPQDEPHEYKEVPITEYSPNRFRMGLYEDVLSLIDLYDAGQSDT
                     ANYMTDLNDALLVISGDIEAAGLSTEDAIKQKEANMLLLESGTDVNGNKTSVTAGYIY
                     KQYDVNGVEAYKDRVRKDIHEISMVPDLTDDNFSGVQSGEAMKYKLFGFEQMTATKQR
                     LFKKGLMRRYRLLFSLKSSISEMDNSDLKGLRVIFTPNLPKAILEELKSLVDAGAELS
                     QETILGLASFVPDVQAELKRVNAETSADRGVFDSDKETDTEVEEDEST"
     gene            8289..8552
                     /locus_tag="NBL02_000927"
     CDS             8289..8552
                     /locus_tag="NBL02_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571632.1"
                     /translation="MKARKKPVTVEIVQLKMLSARSYRKCKEFVGEAWIDHNNMPNGL
                     PGIETLEGTMEISDGDYIIKGVHGEFYPCKPDIFIETYEIIEE"
     gene            8557..9513
                     /locus_tag="NBL02_000928"
     CDS             8557..9513
                     /locus_tag="NBL02_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCD2571633.1"
                     /translation="MKSQDYFIKREKAWQEQQIKDDKKRMNEIKKRLQYAQDAIQKEI
                     DAQWNSFSNGQKITRSEAMKRANEMDVKAFARKAKKYVKEKDFSPTANKELKLYNLTM
                     RVNRLELLKANIGLELIATFNELDKYFSNELTKAGLDELKRQAGILEMAIAKSGYAKL
                     VEQVINSSFRADGFATFSERLWMYQAELKADLDKLLVRSVTMGRNPKQLAPELSKFLT
                     EEGRENTKFNTQRLMVTETTRVQSGIQRESYLDANIEKYGWVTEPNACRLCKSIAADG
                     PYLVSEMEVGKNMMPVHPFDRCSTYPIVDRDAFEKSLKERRL"
     gene            9591..9818
                     /locus_tag="NBL02_000929"
     CDS             9591..9818
                     /locus_tag="NBL02_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571634.1"
                     /translation="MHLYQNDAQFKEDYDAVKQHLIDVLEPIVKEVYETARKVIEPVV
                     QSITELVQSNPEYFKALQNNMNQSVFQSASM"
     gene            9833..10006
                     /locus_tag="NBL02_000930"
     CDS             9833..10006
                     /locus_tag="NBL02_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571635.1"
                     /translation="MENEEAIVRAIASLNKILQNEKVLRVSVETDSEWEDGIQSIDIS
                     VKYLPSGDVSEAE"
     gene            10121..10723
                     /locus_tag="NBL02_000931"
     CDS             10121..10723
                     /locus_tag="NBL02_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HCD2571636.1"
                     /translation="MKKRLFMPMNLQFFSEPGDGGSGDEGQQGNLPAGSQETPTEAKE
                     ENNTGKTFSRDEVAKMIAAETNKAKAAWEKELEAKKEEAKKLAKMNAEEKLQHELEQK
                     EAEIAELKRGQALSEMTKEASKMLTDANLPHDDDLLGLIVSDDADATKQAVAVITNFA
                     SLIKRENARQTPPNEGGQFTASKNTKETVAKLAAKNRIIK"
     gene            10738..11625
                     /locus_tag="NBL02_000932"
     CDS             10738..11625
                     /locus_tag="NBL02_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCD2571637.1"
                     /translation="MAQTWNPDNVTVYETKEGKIPDKYNTLIINDIMENSKVMQLAKY
                     EEMTDKEKKFEYFAEGPGAYWVGEGEKIQTSKPKWLQATMVAKKLGVIIPVSREYLHY
                     KMSDFFTVMQPKIAEAFYKKFDAAALLNTDNPFPQSLEESVVAAANVIKGPLTYDNIL
                     ALEDALGKNEFEPNAFISNRKNRTELRSAAQTVGTNVEFIYDRSANTIDGLPVVDLKA
                     LGKGELYAGDFNYMFYGIPFNISFKISEDAQLSTLKNEDGTPVNLFEQELVALRATMD
                     VGFMIVKDAAFGKIEPAGE"
     gene            complement(11582..11869)
                     /locus_tag="NBL02_000933"
     CDS             complement(11582..11869)
                     /locus_tag="NBL02_000933"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571638.1"
                     /translation="MCDPSSVFIVVVYLPAGTVSVQVILPLEETVNPLVTGAIEYVTV
                     LFVAFCGATVAVNCLLPAVPATALEVLGVTVTPITGIVTPPPVQFYRTQHL"
     gene            11919..12248
                     /locus_tag="NBL02_000934"
     CDS             11919..12248
                     /locus_tag="NBL02_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HCD2571639.1"
                     /translation="MVIKDDVKKLLSGSTDDKLEVIERRTRERLASLLGVSVTPESLE
                     YIVFDVTNKRFNRIGQEGMTSYAQEGLSMAFPDSDFSEYASEIDDFKRKDDEELYKPR
                     KGSVRFL"
     gene            12245..12559
                     /locus_tag="NBL02_000935"
     CDS             12245..12559
                     /locus_tag="NBL02_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571640.1"
                     /translation="MRFLDEVIFEKDGEGNRYDPELGEWIEEAPITVTTNANVTDLGT
                     DRSVAIFGDIRQGAKVIRTMPLFVVPKYDRIIFNGKTYKDVAQRSLLSTNSIIVQEVI
                     AK"
     gene            12556..12927
                     /locus_tag="NBL02_000936"
     CDS             12556..12927
                     /locus_tag="NBL02_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HCD2571641.1"
                     /translation="MKVSLGYKGVDELLKHLEEAVTLRDVQMVVKTNGAELTKRMQEK
                     ARFNGHWEGDVFVHPTGFTRRSIRMWLLEGGFVAQVGPQSDYSPYLEYGTRFMSAQPF
                     VGPAFNVQKAIFMKDMQRLFK"
     gene            12924..13313
                     /locus_tag="NBL02_000937"
     CDS             12924..13313
                     /locus_tag="NBL02_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HCD2571642.1"
                     /translation="MIKTRDQSLFDEMFARSLALGYQTYDYKPLDDVGYPFVELESSQ
                     TVHNPNKTDIKGTVNLTISVWGLQKKRKQVSDMASALFNQALQIQATEGYYWDLNYQA
                     SGIQVMDDTTTNTPLKRAVVTLEFRIR"
     gene            13324..13836
                     /locus_tag="NBL02_000938"
     CDS             13324..13836
                     /locus_tag="NBL02_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HCD2571643.1"
                     /translation="MEALKGIDVILLYRLLKKETQEAAWKMAFQTEHENGLSRDSDST
                     VTKDGNVQSLSPVEYDFSATSIVAKGDSHVDEMKQALLNGDIIEIWEINKAEQGTDDD
                     ANKYKATYYQAYVSEFTPSAAAEDNVELSLSFAVNGVGQDGYATLTEDQADVVQYAFK
                     DTVKATSTGA"
     gene            13882..14250
                     /locus_tag="NBL02_000939"
     CDS             13882..14250
                     /locus_tag="NBL02_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HCD2571644.1"
                     /translation="MKLKIKGKEYSFKFGTKFVRELDKVMPFIDGNMEFGMGLSAKVL
                     PELRSYNANTLSRVLEIANRTEEETITLDEMDDYIDEVKDIEKLFDEVLKELAESNAG
                     KLAVRNLNQKLKEAEKQQAE"
     gene            14304..14591
                     /locus_tag="NBL02_000940"
     CDS             14304..14591
                     /locus_tag="NBL02_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571645.1"
                     /translation="MTNISDIERMTLYEYNIRMTAAQLSWLDKEKLIHELAWANQQVQ
                     AEKKVGKKTVPVYRSFEEFFNYQKIEDSIMGVSELSKQDKKFQSLLTKANS"
     gene            14608..17430
                     /locus_tag="NBL02_000941"
     CDS             14608..17430
                     /locus_tag="NBL02_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCD2571646.1"
                     /translation="MEQFSVEALLKATDSGFVKTFKDAQDAVKTFEEKSNSMTTAVGK
                     VMQGTGAAMTKYITTPLIGVGVAAAKVGGDFEEQMSRVKAISGATGDTFEQMKQQAID
                     LGAKTAFSAKESAAGMENLASAGFSAQEIMKAMPGLLDLAAVSGGDVALASENTATAL
                     RGFGLEASEAGHVADVFARAAADTNAEVGDMGEALKYVAPVANSMGISLEETAAAIGI
                     MSDAGIKGSQAGTTLRGALSRLARPTKAMQDTMDNLGVSFYDADGKMKPLKTQVELLK
                     KAFEGLTPEQQQNALVTLYGQESLSGMMALIDKGPDSLGKLTKSLKDSDGAADDMART
                     MQDNMNSSIEQMFGAFESAAIVIQKILAPSIKKVADAISGLVEKFVSAPESTQRLVVA
                     IGAIAIAIGPVLYALGMLVKAFQTMKVGLGVLGNGISLFKKLGSAIGFLTSPVGLVIA
                     AVALLVVGFIYLWNTSEDFRNFWIGLWEGIKSAVSSAVEWIQNAWKSTGEWFNNLWKS
                     IKEGADNVWTTIQEAPGKAADWIKNKWTETKEFFSSIWDGIKEAASSAWEGIVNILTP
                     YVIAIKNVFQPMIDFFTNLWSQIGSIAGSAWEIIKTAVMGPILLLIDLITGNFNQLKE
                     DASMLWTTLTTNIQNIITTFVDIVVGYYTALKDTVINIWNVLTSTIKDVWNSFTTWIK
                     ETTNNIVNSIKQGWNNLKQGTIDLFNNMIQGAKDLWNSFKAWFINLVIGTKDNIIQGW
                     ENLKQGTIDTFNNLVNGAQEVWDNLVNAVSDTVDRVTGWFDNLKNIDLLAAGKAIMDS
                     FLEGLQNAWKSVQDFVGGIGDWIREHKGPIRYDRKLLIPAGQAIMNGLNAGLTNGFAS
                     VQSNVGNMANMIADSFTRTPDIDLSANLKNANRNFTTQIEHSVNYGKNKRPAVFNIRL
                     GNQVFEAFVEDISNIQGKEADINLLF"
     gene            17448..18230
                     /locus_tag="NBL02_000942"
     CDS             17448..18230
                     /locus_tag="NBL02_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HCD2571647.1"
                     /translation="MEWHNPMYEFRDTVKNDSQKTWIPTSALNYDGKFIENYIEGYQT
                     LYVEGREMVSLEIESEAVSIGVRISSQRLPERILTIHFKLEEKNPIEFQRSFNKLMRL
                     LYRDKDVEIHFNDELDMYYYGRYQTCDNIPGNVHSVISSFSIICSDPRKYTRIFETNG
                     IVSEYLPYEVAPISISLKANNDGSLRITNGRQNISVTNSMIKKGDFIEMDIAEGKVFV
                     NGVNKTRILDLTSSFKNFMVRTGDLVKCDNGTPLIRYRGVWL"
     gene            18230..21004
                     /locus_tag="NBL02_000943"
     CDS             18230..21004
                     /locus_tag="NBL02_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCD2571648.1"
                     /translation="MDKDVYFFDDSQKLIKIVGEDKLFSVVQEKEITPSKDELINDKL
                     AVSMEFDDEIKESAYMAVRESESSFSMYKIIGIADPGSLLIFTGINFGPDELDAYIIN
                     DIRPANEFFQKTIQRVIDFTLGEWRVGHLDSTLPAVSMTFYYCSIREALKNLQTLGCE
                     IVFRCNLSGEGITDKWIEVYKQIGEYSNERYEYGDKALTIEKEVDRSNIYTSLIGRGR
                     GEEVGDGYGRRIEFDQVYWSKSKGDPLNKPTGQIYLEIPEMTEKYGIPTKNGKRRKRE
                     KVIIFEDCEDPVELIQLTYQELVNCSRPLVQFKATIFGADSLGNIIRIHRDDRGYHYE
                     TRIFSVKIDRLTGKVETGLGDNLNTSSTRQASNTQTAIQTLDEKKMTFYESTEVSKWQ
                     SDIIRGAKGGSIIMMNPWDTGKGESRQPYQMVWMNGDSIDTSNHFLVANSEGIGFIDG
                     KFNESNFKTAWTIDGNFNANYIQSGRIRADIFETSFNAVGDQLKLVKGALQIVNSNKK
                     IMELTKKGMEFWNTKESIGTIGTTDSAGNPFPGASTPTPIEDNSLVIRTNGDGKYILI
                     SPKVGKGLVLLGNGKAIYFGDLDVQGKLTVNGKEITGNNSGGSDPGTIPPQLTTEAEK
                     RAWKIWTMLKARGYSEYAAAGILGNIQGEVGASMNPDTEQLGGPAYGIVQWDGSAYPL
                     VGSPTWNGREYVQRLMNTAGIQEDYRSIEAQVKLLDWCMFNGQWLGKVNPTTVSGFKS
                     INDAKSAAYAFEMNFERPASAHPERQNYAQSWYNKLHGLTSPEPGGNFICPIQKPVTV
                     TSECGWRTSPINGGQEFHNGIDLVNGNPNTPVFAALDGEVVQAGANYYDWYGNYVVIK
                     HNNGKWTGYAHLSRIDVSVGQKVQKGAQIGLMGTTGPSTGEHLHFQIMKNYWPQPVVD
                     FENPRNYIQF"
     gene            21017..21925
                     /locus_tag="NBL02_000944"
     CDS             21017..21925
                     /locus_tag="NBL02_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HCD2571649.1"
                     /translation="MSKWNVVLSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            21925..22545
                     /locus_tag="NBL02_000945"
     CDS             21925..22545
                     /locus_tag="NBL02_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571650.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            22549..22998
                     /locus_tag="NBL02_000946"
     CDS             22549..22998
                     /locus_tag="NBL02_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571651.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            22998..>23225
                     /locus_tag="NBL02_000947"
     CDS             22998..>23225
                     /locus_tag="NBL02_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571652.1"
                     /translation="MADIVQLKEDGVAKYLKTHVKAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTG"
BASE COUNT         8124 a         3592 c         5167 g         6342 t
ORIGIN      
        1 gagcttatgg attgattcaa aaggacgaca gtttaggtat gtggaggaga gataatggat
       61 ctcattacac aatacagtga catcatcctc aagaaaatca tgatgaagat tcagaaagac
      121 aaaaaatcaa aagaacgagc tgaattagtt aagttagaaa tggctgaaac aggagcagga
      181 gtgcgaagta gcaggcattg gaaagcagca gcaaacattg aattttatta caacgaaatt
      241 caaaaagggt tcgatcagat gcgtgagctg gatcggcaaa caaattggag caagaaactt
      301 catcaagatc gtttcaaatt tgtagagaag tataaagaaa tattagaaga gtatttgagg
      361 aggacagcaa atgataaaaa aactcgttca attcagcatg gatttatatg atatcgaatc
      421 aggagcaaca ctatctgtgg aatcggacca tctaatcata aattttggtg gaaagcgcca
      481 gattattttg tgggtagttg atgatgtact gtttccagaa attgttcatg atttcgaaga
      541 atcaaaagcg gttgagtttg aaatagtgaa aaaagtaatg gaattgattg aaaaatacga
      601 ggaggacagc gaatgatacc gaagtttaga gcgtgggtaa aacctgatat tttaagcaat
      661 catcttaatg gggtgattgc tgaggcatta cctaatttta tttatggcaa ttgtttagtt
      721 ttgagggaag atttagaaga agataagcag tttgaagaaa ttattgattt tcttgatatt
      781 gaactcatgc aatccacagg aatgaaagat aagaatggtg tggatatatt tgaaggggat
      841 attgtcaaaa ctctcgatgc tgatttgaaa gtaacttttt caacgataaa gtttgaagaa
      901 ggcgcatttt gtgttgatta caaagatctc ggcacggaat ttgatttttt gtactttgta
      961 gattcacctt tggaagtcat cgggaatata tacgagaata gcgagttatt ggaggaacag
     1021 cgatgaataa acaggaattg attgataaac tatcaagcct tgtaggtagc atagaggact
     1081 ttaaaggtat aagtactttt catgctggaa aatatgcggg tttagaacac gcattagaac
     1141 taatccaaca actaaatgaa tcgcagaaac cagttattcc acaactcgtg gcaggttgga
     1201 tcgaaaaaag taccgacaat ttaacaaaag ctgaaaaaat agcgtattta atcaaatcca
     1261 aagatggtga ttcatattat ttctgtgatt ggtttgtgcg agatggcata ttgacgcaag
     1321 aacaaggaga agaattactc gcttgggcaa cgagacaatc atatgaaaca ctattgagcc
     1381 tatacaacgg ctacgaggtt gagaaagaac cgttgtatga agttattatt ggtgacttat
     1441 atcttatcaa gaaatttaat aacagaaatg atttctgttt tgatattagt cgctcgctat
     1501 gtacttggga aaaatctgct tatcagctaa cagaagcgga aatcaaagaa attgatgaaa
     1561 gatactggcc atttgctgtg ccagtggaag aggtggtaga aagatgagcg attacttaac
     1621 agcagaacaa tatgaatatg ctagaaaaac aggagattgg gaatcgggta aagttctcac
     1681 tcatggacaa gcgctcaacg acaaccagaa aatcgtgttg gagtggttag aagaatatta
     1741 cgcaaaatca caatggccgt ttccttttta tgtgttaaag catgcagcaa acgatatgcc
     1801 gacgaatatt tttggttttt atgatgattt tactatgaaa gaagaatttg aagttctagc
     1861 agcattcgct caatggggat taaaacagga ggaagcggaa tgaataaacg ccaaaaaaat
     1921 aaacaattta aaaagcagtt aatcaaaaac ggttatccag ataagaaagt gaaactttat
     1981 tgttcagaat gtggaaacag attgagtttt aagaatgaat accataaaag acatctattg
     2041 tgtaacgaat tttgctttat gcatagcgta ggtcttgggt ggaatgattt tctatgatga
     2101 ggaggaagcg gaatgaatgt tcaagtttat ttaaaaagag atttggccag gtttcctcga
     2161 agctgggatg aatattcctg cccattcgag gcagaagaag agagctatct aatgtctgct
     2221 aacatgatag aaattacaag agatgatgcg gatgagtttg ataaaaaaag atcatggtac
     2281 tcggtttcga atccaatgta tacggctgta atagaagatt atcatatatc cgacagattt
     2341 attctggttg atctagaaag acctaagaaa cgaattaaac agtatagacg ttgcgggttt
     2401 tcaacaaaga aatggtgaaa attaaaacag gaggaagcgg aatgaaacta aaagacggat
     2461 tttactccag cagccacggc atcggcggtt taatgctaga tatgccgaca aagaacccta
     2521 aaacacgtaa gaaaccaaaa ttcaaagtcg gcgacatggt tcgatgtgaa gcagaaggat
     2581 tcatctatcc atttcgtgga tatgtagaac atgtttataa tcactcagcg atcattcgta
     2641 ttgaaaacac gatggaatgc gacaagtggt tagcgaaaag caaagagaat ttagctgtag
     2701 cgagattggt ggatattaaa ctaatcaatg acaaataaaa aagccggatc gctccgactg
     2761 atctaataaa ttccacaagt ttattatatc acataaagga gcggtttgac ttgatgcaat
     2821 tgttacgaga ggtagatttc aaacagacaa gatgtaatgc gagagatgtg ctgaagaact
     2881 ttcggcgctt ggagcggatg gcaggtcgct ctttgataga cattaagtcg cctatcataa
     2941 ccgatatgcc gaaggcacca aagcacgtca ataaggcaga agacgcgatc attcaaatga
     3001 tggatataga agcagagaga gacgcgattt tagcggcttt gatggctctc agtctgatta
     3061 gtcgtcagat actctactac agcttctgtg acgtaaataa gcactctaat tatgaaatag
     3121 gtcaattgat acgaggatac ggagagaaga atgtagagaa gctgaaatcc atcgcattga
     3181 tcgaatttgc tgaagcatac aaaaaaggcg tattagttca gtatcgttga ttttgtaggg
     3241 tttttgtagg gatagtgtag ggtttttgag tgttttaacg tgatattatg gtagtgtcga
     3301 aagattagtg ataggtctga gacaaaataa taataaaagg aacatcgttt tattattgtt
     3361 tcacaattaa gcttcgatag acagcaacgg aaatattaag aataaggatg tgaatttcaa
     3421 ctccttctga ttgttcttat tatctatcat ccgttgctgt ctattaattt atgtattgga
     3481 gggaaaacga atggataaag aaatcaaagc aactgtcaaa ttagatttga ctgaactaaa
     3541 agaactgctc aacaaggcta gtgaccaagt cgaacaaata aagtatcaag gtatggatcg
     3601 aaaacggttg attgatatta ttgagcaaca acgtttagaa ttgatccaaa aagaagctgt
     3661 atgtaaagag tacaaaaaac atttagaaca agtaattgag tatcattcag tagaaaagta
     3721 cagatcagtg gtgcaaaaaa atagagcaac gagtcctgat cctcgtcaag atgtatcgat
     3781 tagtatcaag acgccagtat ttaattctgt taaatcagaa aagctagaaa gtatctcaac
     3841 tgctttggta gaaaaagcag ctgaaatgaa ctccctagca aatgaagtta gtcgtcttat
     3901 ttagtcatat cgataacttg agagtctttg gtgattccgt tttttataga attaatggtt
     3961 tgttcagcgg aatatttagt caagtatgtt tcgctagttg ctactacttc attgttattg
     4021 gacttaataa cgaagtaata ttggccatta gttgcttctc ttataacaaa gtacaaattt
     4081 ttcaccacct ataatttatt tcagcggacc actcgctgat aattaaaatt atacgcttag
     4141 tatttatttt cacaatataa atttgtcact gtggcggaaa ggtaacgcta ataacttaaa
     4201 tcagtggtaa tgagcacctg agcttagttt atgcagctag gcaagagacc gtcgagggta
     4261 aaggacaaga ggcatcttga aataaatccc ctaaagatag acaattctag aggtgagagt
     4321 ttttatgatg gagagtctgt aaaatatcgg aaaatggtgt tgcaatgttc gattcattgc
     4381 cagtgacttt ggtttacggt gatccataac tgcctgtcag taaaaccgaa tatacaggtt
     4441 ggaaactata gcgcgctgat gtttctgtaa tccacattcc ctcgaaggat agtgggtcct
     4501 gtattacata ttagatcact cattgagtgg tctttttatt ttgaaaggag ttttatctat
     4561 gaatgaaaac caattaagag agttatttaa aacgaatgaa gcaaaccaaa ccatggaggc
     4621 gacattctat gaaactcaaa aaagcttagc gttaatcgca aaacaagcta agtatttcta
     4681 cgatcagctt attctacaag ggtttaatga aggacaggct atggaattta tgatgcgaac
     4741 cttttctgcc aataaccaac agagagagtg atacataatg agaaactact ggtatgtatc
     4801 attatcaaat aaatatccgc aaccaaacgc agatgatcca attagagttg tccaatcagt
     4861 ccaaatcaaa aagaagtact ccatcattga aatgaccaga gaagctacac cgaaagagat
     4921 cgataagtac aatcttcgtt actgtggcca tggatatttt agtgagcaga acatacagac
     4981 aaatattgag agatattgtt aataaaattg aaaggtggtg gcttgaatgt ggtaaatttg
     5041 acaccaaaac aaaaagcttt tgcggatgaa tatataaaaa atggtggtaa tgctactcaa
     5101 gccgccatta aagcaggtta tagcaaacga tcagcaagag taataggtaa ggaaaaccta
     5161 actaaaccta acataataca gtatattaat gaacggctaa atcctatcga aaagaagcgc
     5221 aaattaagtg ctgaggacgc tttaaatgaa ttgatagata tttggcaagg agaagtacaa
     5281 ataagcgtga gcaagcaaat agaccgcttg gagaaaaaca aggttattaa acatatgcaa
     5341 tatgaatata caccagattt agaaagcaaa ttgaaagcct tggatttgta tttgaagtat
     5401 aaatcgctgt tatcacaaac gcaattagaa aaagctcaaa cagaaataaa attaatgcaa
     5461 gcaaaattag aacaattaca gataaactca gagcgttcta ccgaagaaaa acttgatgag
     5521 ttgttagaaa agattagtgg tgaattagat gggactagtt gatatttata acccaaagca
     5581 aatcgacgtg ttaaataaaa ccattaagaa tgattggttt attactttat tacatggagc
     5641 aaaacgttct gggaaaacaa aaataaacaa tgatttgttc ttgtttgaat tgcgacgtgt
     5701 gcgaaagatt gccgatgaag aagacattaa ggaaccaatg tatatcctag caggagtttc
     5761 aagtgcaaca atccaaaaga acatcttaca ggaactatac aacatgtaca gcatagaacc
     5821 taaattcgat aaacatggaa actttaagtt atttggcgtt aaggtcgtac aagcttatac
     5881 aggaaacatt ggcggtgttg gtgcaattcg tggtatgaca gcatatggcg cttatatcaa
     5941 tgaggcatca ctagctagac aagaagtatt tgctgaaatc gtttcacgtt gttcaggaac
     6001 aggagcgaga atcctagctg acacaaaccc tgacaacccg gaacactggc tgaagaaaga
     6061 gtatatcgac aattcaagta aaaacattca atcgttccac tttgaattgg atgataatac
     6121 ttttttatct gaacgctata gaaataatat taaagaatca acaccaagcg gcatgtttta
     6181 tgatcgtgat atcaaaggtt tatgggtttc tgcagaaggt gtcgtttatc gtgattttga
     6241 tgctagcaag cactacatcc agtcaaaaga cttaccacct ttgagcaact tttattgtgg
     6301 tgttgactgg ggatatgaac actggggttc aatcgtagtt ataggtgaaa cggatgacgg
     6361 aacagcttat ttaatcgaag aacatgctac tcaatttgaa gagattgatt attgggtaga
     6421 tatagcaaaa ggcattcaag agcgttacgg cttacgagtg cctttttatt gtgactctgc
     6481 gagaccggag catgtggcta ggtttgtaag agaaaagatt aatgctaaaa atgctcataa
     6541 agcacggtta tctggagttg aggaagtcgc caagagattt aaacaagata aattgtttat
     6601 ctgtcaagat agagtgatga aatttcggga tgaaatttat caatacattt gggacaagaa
     6661 aaaaggtgag ccaataaaag aatatgatga tgtgctagac tccgttcgat atgcgatata
     6721 tactcatgag cttcttaaga aaccaaaagt taatgtcaac gaaaagatta aacgtgttaa
     6781 acgtatgttt taaggagtgt gagaaatgga taaggtaaac gaatttgaat acggtactga
     6841 tatacattat tcgaacgacg tgaacacaaa ttatgtaaag tttagcgtag aatcaaatct
     6901 tcactataga tttagctcag cagaagattt acttaacgat tcaaatactt tagcagcaat
     6961 gataaaacat catcatgaat atcaggtaaa gcggcttagt gtattagatg attattacaa
     7021 agctagaaat acaaatatca tggataaccg tagacgtaga gaaaaggaaa aagcggatca
     7081 ccgatcagca cataactttg gaaaagttct ttgtacgttt gatgttgggt acaacacagg
     7141 caatcccata aaagtgcaaa tcgaggatac aaatcaacaa aaagaaatcg aagagtttaa
     7201 tactaataat gacatagatg ggttaaatgc tgaactctgg cttgatatgg ataagtatgg
     7261 gagagcctat gagattatct atcgagattc agatgataca gattatgttg atttggctaa
     7321 tgtatttgaa acatttgttg tatatgatac tacagtaaaa cgagagccta ttttggctgt
     7381 acggtatcct aagacaagat tcaacaagga tgctgataaa cagtacattc aaccaatcgt
     7441 atacacaaaa gaaaaaagta tcacttatga tgagacgaca ctaacagcaa ttgagttaaa
     7501 aaatccccag gatgaaccgc atgaatataa agaggtacct attacagagt attctcctaa
     7561 tcgttttcgg atgggcttgt atgaagatgt actatctttg attgatctat acgatgcagg
     7621 gcagtctgat accgccaact atatgactga tctaaacgat gctcttctag ttattagtgg
     7681 tgatattgaa gcagcaggac tatccacaga ggacgccatc aagcagaaag aagcgaatat
     7741 gcttttgctt gaatctggaa ctgatgtgaa cggtaataaa acaagtgtga ctgcaggata
     7801 tatttacaaa caatatgatg tgaacggtgt agaagcatac aaagacagag tacgcaagga
     7861 tatccacgaa atctcaatgg ttcctgatct tactgatgac aatttttccg gagtgcaatc
     7921 aggagaagcg atgaaatata aattatttgg atttgaacaa atgacggcaa caaagcaaag
     7981 gctattcaaa aagggtctta tgcggcgtta tcgtcttttg tttagcctaa aatcaagtat
     8041 ttctgaaatg gataactccg atttgaaagg cttacgtgta atatttacgc ctaatctacc
     8101 taaagccatt ctggaagagt tgaaatcttt ggttgatgct ggagctgaac tcagtcaaga
     8161 gacgatctta ggactcgctt cttttgttcc agatgtacag gcagaattaa aacgtgtgaa
     8221 tgctgaaacg tcagcggaca gaggtgtgtt tgacagtgac aaagaaactg atacggaggt
     8281 tgaagaagat gaaagcacgt aaaaaaccag ttactgtcga gattgtgcaa cttaaaatgc
     8341 tatctgcacg ttcatatcgc aaatgcaaag aattcgttgg tgaagcatgg attgaccata
     8401 ataatatgcc caatggatta cctggtattg aaacattaga aggcacaatg gaaatatctg
     8461 atggtgatta catcatcaaa ggcgttcatg gtgaatttta tccgtgcaaa ccagatatct
     8521 ttattgagac ctacgaaatc atcgaggagt gattaagtga aatcacaaga ttacttcatc
     8581 aaaagggaga aagcttggca agagcaacag attaaagatg acaagaaacg catgaatgag
     8641 atcaagaagc gtctgcaata cgcgcaggat gcgatacaga aagagataga cgcacaatgg
     8701 aatagttttt caaacgggca gaaaattact cgtagcgaag caatgaagcg tgctaatgaa
     8761 atggatgtaa aagcatttgc tagaaaggct aagaagtatg ttaaagagaa agacttctca
     8821 cctacagcga acaaagaatt gaagctatac aatctaacga tgcgtgtcaa tcgactggaa
     8881 ttgttaaaag ccaatattgg tttagagctt atagctacgt ttaacgagct agataagtat
     8941 ttctcgaatg aacttacaaa agcagggcta gacgagctta agcggcaagc tggcatctta
     9001 gaaatggcga ttgctaaaag cggttatgca aaattagtgg agcaagtgat aaacagctcg
     9061 tttcgagcgg atggatttgc aacgttcagc gaaaggctat ggatgtacca agcagagttg
     9121 aaagcagatt tggataagtt gcttgttcga agtgtgacga tgggacgtaa tcctaaacag
     9181 ctcgctccag aattaagtaa gtttctaaca gaagaaggac gagaaaatac taagtttaat
     9241 acgcaacgtt taatggtaac tgaaacaaca agagttcaaa gcggtataca gagggaaagt
     9301 tacttagatg cgaatattga gaaatatgga tgggtaactg aacctaatgc ttgccgatta
     9361 tgtaagagca ttgccgctga cggtccatat ttagtgagtg aaatggaagt aggtaagaat
     9421 atgatgccag tacatccttt tgatagatgt tctacttatc cgattgttga tcgtgatgct
     9481 tttgagaagt cacttaaaga aagaaggtta taacaatgaa gaaagattca tttcaagtta
     9541 tatctgacag atttttagaa acggcatatg ggaaaagcaa agaggaaatt ttgcatttgt
     9601 atcaaaatga tgctcaattt aaagaagact atgatgccgt taaacagcat ttaattgatg
     9661 tcttagaacc aatcgtaaaa gaagtgtatg aaacggctag aaaggttatt gaacctgtcg
     9721 tgcaatcaat aaccgaattg gttcagtcta acccagagta cttcaaagct ttgcaaaaca
     9781 atatgaatca aagcgtgttc caaagcgctt ctatgtagag gagagtacgc atatggaaaa
     9841 tgaagaagca attgttagag ctatcgcaag tttaaataag attctacaaa acgaaaaagt
     9901 gctaagagta tccgttgaaa ctgatagtga atgggaagac ggtattcaat cgattgatat
     9961 cagcgtgaaa tatttacctt caggagatgt aagtgaagcc gaataacggg ctttttatta
    10021 tgccctctac tgctcggggc aatataaatt gagctgttcc gactgaccgg cgtaactggt
    10081 caaatttatc gggtagcggc gtaaccgtgg aggattaatc atgaaaaaac gtttatttat
    10141 gccaatgaac ttacaatttt tttctgaacc aggagatggt ggatctggtg atgagggaca
    10201 acaaggaaac ctaccagctg gctcacaaga gacaccgacc gaagcaaaag aagaaaacaa
    10261 tactggcaaa acattttctc gtgatgaagt agcgaaaatg atcgctgctg agacgaataa
    10321 agcaaaagca gcgtgggaaa aagaactaga agcaaaaaaa gaagaagcta aaaagctggc
    10381 aaaaatgaat gcggaagaaa aactacagca tgagttggaa caaaaagaag ctgaaatcgc
    10441 tgaattaaag cgtggacagg cactatctga aatgacgaaa gaagcttcta aaatgctgac
    10501 agatgcaaat ttaccacacg atgatgattt acttggtctg attgtttctg atgatgcaga
    10561 tgccacaaaa caagctgtag cagtcatcac taactttgct tctttgatta agagagaaaa
    10621 cgcaagacaa acaccaccaa atgaaggtgg acaatttaca gcatcgaaaa atactaaaga
    10681 aacagtggct aaactagctg ctaaaaatcg aattatcaaa taggaggaat aaacaatatg
    10741 gcacaaacat ggaatccaga taacgtaaca gtttatgaaa ctaaagaggg taaaatccct
    10801 gacaagtata atactttgat tatcaacgac attatggaaa actcgaaagt aatgcaattg
    10861 gcgaaatacg aagaaatgac agataaggaa aagaaatttg aatactttgc tgaaggtcct
    10921 ggcgcatact gggttggtga aggtgagaag attcaaacat ctaaacctaa atggttgcaa
    10981 gcgacaatgg tagctaaaaa gctaggcgtg attattcctg tgtctcgtga atacttgcac
    11041 tacaaaatgt cagatttctt cactgttatg caacctaaaa ttgctgaagc tttctacaaa
    11101 aagtttgatg cggctgcatt actaaacact gacaatccgt ttccacagtc tttagaagaa
    11161 tctgtcgttg cagcggctaa tgttatcaaa ggtcctttga cttatgacaa cattttggca
    11221 ttagaagatg ctttaggcaa aaatgaattt gaaccaaatg cgttcatctc aaatcgtaaa
    11281 aaccgcacag aattgcgttc tgccgctcaa acagtcggta caaacgttga atttatctat
    11341 gaccgctcgg cgaatacgat tgatggtctt cctgtggttg acttgaaagc attgggtaaa
    11401 ggtgaattgt acgctggtga ctttaactat atgttctatg gtatcccatt caacatttcg
    11461 ttcaagattt cagaagatgc gcaattatct acgcttaaaa acgaagatgg cactcctgta
    11521 aacttgtttg aacaagagct ggttgcgttg cgtgcaacaa tggatgtcgg ctttatgatc
    11581 gtcaaagatg ctgcgttcgg taaaattgaa ccggcggggg agtaacaatc cccgttatag
    11641 gcgttactgt aacgcctaaa acctcaagcg cagttgcggg gactgctggc aatagacaat
    11701 taacagctac cgttgcacca caaaacgcaa caaataaaac agtgacgtat tcaattgcgc
    11761 cagtaacaag cggattgacg gtttcttcta gcggtaatat cacttggact gacacagtac
    11821 cagccggtaa atacacgaca acaataaaaa cggaagatgg atcacacaca gatactcacg
    11881 ttttgacact gactgagccg taggaaggat aggtacgaat ggtaattaaa gatgacgtta
    11941 agaagcttct aagcggttct acagatgata agctagaagt tatcgagaga cgaactagag
    12001 agcgcttagc gtcattgcta ggcgtttctg ttacaccaga atcactagaa tacatcgttt
    12061 ttgacgtaac gaacaaacgt tttaaccgaa ttgggcaaga gggcatgact tcatacgctc
    12121 aagagggttt atcgatggct ttccctgatt ccgatttttc agaatacgca tcggaaattg
    12181 atgatttcaa acggaaagat gacgaagaat tgtacaaacc tagaaaaggg agcgtgcggt
    12241 tcttatgaga tttttagatg aggttatctt tgaaaaagat ggagaaggaa atcgttacga
    12301 tccagaatta ggcgagtgga tagaagaagc accgattacg gtaacaacaa acgcaaatgt
    12361 aacggactta ggaacagata gaagtgtggc gatttttggg gatatacgac aaggggcgaa
    12421 ggttatcaga accatgcctc tttttgttgt tccaaaatat gaccgcatta tttttaatgg
    12481 aaagacatac aaagatgtcg ctcaacgcag cctgttatca acgaatagca ttatcgtcca
    12541 ggaggtgatt gcaaagtgaa agtttcattg ggttacaaag gtgtggatga gctgcttaaa
    12601 cacttggaag aagctgtcac gcttagagat gtgcaaatgg tagttaaaac aaatggagcc
    12661 gagcttacta agcgcatgca agaaaaagcc cgctttaatg gacattggga aggggatgtg
    12721 tttgtgcacc cgacaggatt cactagacga tcaattcgaa tgtggttgtt ggaaggcggt
    12781 tttgttgcac aagttggacc gcagagtgat tacagtccgt acttagaata tggaactcga
    12841 ttcatgagcg ctcaaccttt cgttggtccc gcatttaatg tgcagaaagc aatctttatg
    12901 aaagacatgc agaggttatt caaatgatta aaacaagaga tcaatcatta tttgatgaaa
    12961 tgtttgctag gagcctagct cttggatacc aaacttacga ctacaaacca ttagacgatg
    13021 taggttatcc gtttgttgag ttggaaagct cccaaacagt ccacaatccg aataaaaccg
    13081 acatcaaagg aacagtcaat ttgacaattt ccgtttgggg attgcagaaa aaacgcaaac
    13141 aagtgtctga tatggcttcg gcactattta atcaagcgtt gcagatacaa gccacagaag
    13201 gctattattg ggatttaaat tatcaagcaa gtggaattca agtgatggac gacacaacaa
    13261 ccaatacgcc cctaaaacga gcggttgtca cacttgaatt tagaattaga taggaggaag
    13321 aacatggaag cattaaaagg tattgatgtc attttgcttt atcgcttatt gaaaaaagaa
    13381 actcaggaag ctgcttggaa aatggcattt caaacagaac acgaaaatgg tttatcaaga
    13441 gattcagact ctacagtgac aaaagacgga aacgttcaaa gtttaagccc agttgaatat
    13501 gatttttcgg ctacttcaat agttgccaaa ggcgattctc atgtagatga aatgaaacaa
    13561 gccttattaa atggcgatat cattgaaatt tgggaaatca acaaagcaga acagggcaca
    13621 gatgatgatg caaataagta caaagctact tattaccaag catatgtgtc tgaatttact
    13681 ccatcagctg ctgcagagga taacgttgaa ttaagtttat catttgcagt aaatggtgtt
    13741 ggtcaagatg gttatgcaac cttgacagaa gatcaagccg atgttgttca atatgcattc
    13801 aaagataccg tgaaagcaac ttcgacagga gcataagagg gcttagatgc tctctttttt
    13861 attttaggag gatgaaaaac attgaaatta aaaattaaag gtaaagaata ttcgtttaaa
    13921 tttggcacta aatttgtacg tgaattagac aaagtgatgc ctttcatcga tggaaatatg
    13981 gaattcggaa tgggactctc agcaaaagtc ttaccggaat tacgttctta taatgccaac
    14041 acattgtcac gagtcttaga aatagcaaat agaacagaag aagaaactat tacgttggat
    14101 gaaatggatg attacatcga tgaagttaaa gacatcgaaa aattgtttga tgaagtccta
    14161 aaagaattgg cggagtcgaa cgcgggaaag ttagcggtcc gaaacctgaa tcagaaattg
    14221 aaagaagcgg aaaaacaaca agcggaatag attctgcact ggcatacgaa caaattctta
    14281 tcaattcttt tcgatatttg gaaatgacca atatctcaga tatcgaaaga atgacgttat
    14341 atgaatacaa cattcgtatg actgcagccc agttatcttg gcttgacaaa gaaaagttga
    14401 ttcacgaatt agcgtgggca aatcagcaag tccaagcgga gaaaaaagta ggcaaaaaga
    14461 cagttcctgt atatcgatcc tttgaagaat tcttcaatta tcaaaaaatc gaagattcaa
    14521 tcatgggagt ttccgaactt tcaaaacaag ataaaaaatt ccaaagctta ctaactaaag
    14581 ctaactcttg aggaaaggag gaaaatcatg gaacaatttt ctgttgaagc cttattaaaa
    14641 gccacagata gtggatttgt aaagactttt aaagatgcgc aagatgctgt taagactttt
    14701 gaagagaaat caaatagtat gacaaccgct gtaggtaaag tgatgcaagg tactggtgcc
    14761 gcaatgacaa agtatattac cactcctctt ataggagtag gcgtagcagc tgctaaagtt
    14821 ggtggcgact tcgaagaaca aatgagtcgt gtaaaagcta tatcaggagc aacaggcgac
    14881 acatttgaac agatgaaaca gcaagcgatt gatctaggag caaaaactgc ttttagcgca
    14941 aaagaatcgg ctgccggcat ggaaaatctt gcttctgctg gatttagcgc acaagaaatc
    15001 atgaaagcaa tgccgggtct tttagactta gcagctgtat ctggagggga tgtggctcta
    15061 gcttctgaaa atactgctac tgctttgaga ggatttggtt tagaagcaag tgaagcagga
    15121 catgtcgctg atgtatttgc tcgtgctgct gcggatacca atgctgaagt tggagacatg
    15181 ggagaggcat tgaagtatgt tgctcctgta gccaattcaa tgggtatttc tttggaagaa
    15241 actgcagcag ctattggtat tatgagtgac gcaggtatta agggttctca agcaggtaca
    15301 acgttgcgag gagcattgtc taggttagca aggccaacaa aggctatgca agatacaatg
    15361 gataatttag gtgtttcgtt ttatgatgct gacggtaaaa tgaaaccttt aaaaactcaa
    15421 gtagaattac ttaaaaaagc ttttgaaggc ctgacgcctg aacaacaaca aaatgcttta
    15481 gtaacactat atgggcaaga atcattatca gggatgatgg ctttgattga taaaggacct
    15541 gattcattgg gcaaattaac aaaatctctg aaagattctg atggtgcagc tgacgatatg
    15601 gctcggacca tgcaagataa tatgaattct tccatcgagc aaatgtttgg agcttttgag
    15661 tcagcagcta ttgtaattca aaagattcta gcaccatcca tcaaaaaagt agcagatgcc
    15721 atatccggct tagtagaaaa atttgtaagt gctccagaat caactcaaag attagtagtg
    15781 gccataggag caatcgctat tgcaattggg ccagtattgt atgcattagg aatgctggtt
    15841 aaagcgtttc aaaccatgaa agtggggtta ggtgtattag gtaacggaat ctctttgttc
    15901 aagaaattag gttccgccat aggttttctt accagtccag tcggattggt tatagctgcg
    15961 gtagcactac ttgttgtagg tttcatctat ctttggaata cgagtgaaga ttttagaaac
    16021 ttttggattg gcttatggga gggaatcaag tctgctgtaa gctcggcagt agaatggatt
    16081 cagaatgcat ggaaatctac aggagaatgg tttaacaatt tatggaagtc cattaaagaa
    16141 ggcgcagaca atgtttggac tacaattcaa gaagcccctg ggaaagcggc agattggatc
    16201 aagaataaat ggactgaaac aaaagagttc ttttcgagta tatgggatgg catcaaagaa
    16261 gctgccagtt ccgcttggga aggaattgta aacattctaa caccgtatgt tattgccata
    16321 aaaaatgttt ttcagccaat gattgatttc tttacgaacc tatggtctca aattggatca
    16381 atcgcaggtt ctgcatggga aattataaaa actgctgtaa tgggtccaat tctacttttg
    16441 attgatttga taacaggcaa ttttaatcag ttaaaagaag atgcttcgat gctgtggact
    16501 acattaacta caaatatcca aaacattatc acgacgtttg tagatatagt tgttggttat
    16561 tacacagcct taaaggatac tgtgataaat atctggaatg tgttgacttc taccatcaaa
    16621 gatgtgtgga attcttttac tacatggatc aaagagacaa ctaacaatat tgtaaatagt
    16681 attaaacagg ggtggaataa cctaaaacaa gggacaatcg atctgtttaa taatatgatt
    16741 caaggagcga aagatttatg gaattctttc aaagcttggt ttattaatct agttattgga
    16801 actaaggata acatcattca gggatgggaa aacctaaaac aaggtactat agatactttc
    16861 aacaatttag taaatggtgc tcaagaggta tgggataatt tagtaaatgc tgttagtgat
    16921 acggttgata gagtaactgg ctggtttgat aacttgaaaa atatcgattt actagcagcc
    16981 ggaaaagcta tcatggatag ttttctagaa gggttacaaa atgcatggaa atctgtgcaa
    17041 gattttgttg gaggtattgg tgactggatt agggaacaca aaggacctat tcggtacgat
    17101 agaaaattgt taatcccagc tgggcaagca atcatgaatg gactaaacgc aggtttaact
    17161 aatggctttg ccagtgtcca aagcaatgta ggaaacatgg ctaatatgat tgcagatagt
    17221 tttactcgta cacctgatat tgatctttca gcgaatttaa aaaatgcaaa tagaaatttc
    17281 acaacacaaa tagagcatag tgttaactac ggcaaaaata aacgtcctgc agttttcaat
    17341 attcgcttag gaaatcaagt gtttgaagcg tttgttgagg atatttcaaa cattcaaggt
    17401 aaagaggcgg atattaattt attgttctag aaagtgaggg aaatatgatg gagtggcata
    17461 atccaatgta tgaattcaga gacacggtta aaaacgacag tcagaaaaca tggataccga
    17521 cgtctgcatt gaattatgat gggaagttta tcgagaatta tatagaaggg taccagactt
    17581 tgtatgttga aggtagagaa atggtttctt tagagattga gagtgaagcg gtgagcatag
    17641 gtgttcgtat tagttctcaa agacttccag agaggatttt aactatacat tttaaattag
    17701 aagaaaagaa tcctattgaa tttcaacgaa gctttaataa attgatgagg ctgctttaca
    17761 gggataaaga tgtagaaatt cactttaacg atgagttgga tatgtattat tacggaaggt
    17821 atcagacctg tgataatatt ccaggaaacg ttcatagtgt aatatctagt ttctctataa
    17881 tctgttctga tcccagaaaa tatacacgta tatttgaaac aaacggcata gtctcagaat
    17941 atcttccgta tgaagtagct ccgatttcaa ttagtttaaa ggctaataat gatgggagct
    18001 taagaattac aaatggccgt caaaatatta gtgtgactaa ctcaatgata aaaaaaggtg
    18061 actttatcga gatggacata gctgaaggaa aagtttttgt gaatggagtg aataaaacga
    18121 gaattcttga tcttaccagc tcatttaaaa actttatggt tagaactggt gatctagtta
    18181 agtgtgataa tggcactccc ttaatacgat atagaggagt gtggctgtaa tggacaaaga
    18241 tgtttatttt ttcgacgata gccaaaagct tattaaaata gtaggggaag ataaactttt
    18301 ttcggtagtt caagaaaaag aaatcacacc aagtaaagat gagttaatta atgataagtt
    18361 agcagttagc atggagtttg atgacgaaat taaagagtca gcttatatgg cggttcgtga
    18421 aagcgagtcg tctttttcta tgtataaaat tatcggaatt gctgatccgg gttcattgtt
    18481 gatattcact gggattaatt ttgggcctga tgaattggat gcttatatca ttaatgatat
    18541 tcgtccggct aacgaatttt ttcagaaaac catccagaga gtcatagatt ttacattagg
    18601 tgagtggcga gttggtcatt tagattcaac tctgccagcg gtttctatga ctttttacta
    18661 ctgtagtatc cgtgaagcct taaaaaatct acaaacgtta ggatgcgaga ttgtttttag
    18721 gtgcaatcta agtggagaag gaatcacgga taaatggata gaagtttaca agcagattgg
    18781 cgaatacagt aatgagcgat atgagtacgg cgataaagcc ttgacaattg aaaaagaagt
    18841 agatcgaagt aacatctata cttctctaat cggccgcggc cgtggtgaag aggttggaga
    18901 tggttatggt cgtcgaattg agtttgatca agtatactgg tccaaatcaa aaggggatcc
    18961 gttaaacaag cctactggcc agatatattt ggaaatccct gagatgactg aaaaatatgg
    19021 tattcctact aaaaacggaa aacgtcgtaa gcgtgagaag gtaattatat tcgaagactg
    19081 cgaagatcct gttgaactaa ttcagcttac ctatcaagaa ttggttaact gttcacgtcc
    19141 gctagttcaa tttaaagcaa ctatttttgg agcagatagt ttaggtaata ttatacgtat
    19201 tcatcgtgat gaccgcggct atcattacga gactagaatt ttcagtgtga agattgatcg
    19261 attaacagga aaagtcgaaa ctggcttagg tgataattta aatacttcat caacacgtca
    19321 agcttcaaat actcaaactg ccatacagac ccttgatgag aagaagatga ccttttatga
    19381 gtccacagaa gtttctaaat ggcagtcgga tatcattcgc ggagcaaagg gtggatcaat
    19441 tatcatgatg aatccttggg atactggcaa aggcgaaagt cgtcaaccat atcagatggt
    19501 ttggatgaat ggggatagta ttgatacttc taaccatttt cttgtagcta attcggaagg
    19561 gattggtttt attgatggga aattcaatga gtcaaatttc aaaacggcat ggacgattga
    19621 tggaaacttc aatgccaatt atatccaatc tggacgtatt agagcagata tttttgaaac
    19681 ttcatttaac gctgttggtg atcagctcaa gttagtaaaa ggagctttgc aaattgtaaa
    19741 cagcaataaa aaaatcatgg aattaaccaa aaaagggatg gagttctgga ataccaaaga
    19801 atccattggc acaattggta ctactgattc tgctggtaat ccttttcctg gagcttctac
    19861 tcccacacct attgaagata attctttagt tattcgtaca aatggagacg gcaaatatat
    19921 tttgatttct cctaaagttg gtaaaggatt agttttatta ggaaatggta aagcaattta
    19981 ttttggagac ttagatgtac aaggcaaact cacagttaac ggaaaagaaa tcacagggaa
    20041 caacagtgga ggaagtgatc ctggaactat ccctcctcaa ttgacgacgg aagctgaaaa
    20101 aagagcatgg aaaatttgga caatgctgaa agctcgtggc tattctgaat atgcagctgc
    20161 aggtattcta ggaaatattc agggggaggt tggagcaagt atgaaccctg atacagaaca
    20221 acttggtggt ccagcttatg ggattgttca atgggatggt tctgcttatc cgttagtcgg
    20281 atcaccaact tggaacggac gagagtatgt tcagcgtttg atgaacaccg cagggattca
    20341 agaagattat cgaagtattg aagcccaagt aaaattatta gattggtgta tgttcaacgg
    20401 tcaatggctc ggaaaagtaa atccaaccac agtatcagga tttaaatcga tcaatgatgc
    20461 caaaagtgca gcgtatgctt ttgaaatgaa cttcgaacgc ccagcttctg cacatccaga
    20521 acgccaaaat tatgcccaat cttggtataa caaattacat ggattaacta gtccagaacc
    20581 tggagggaat ttcatttgtc caattcaaaa accagtgaca gttacttcag aatgtggatg
    20641 gagaactagt ccaataaatg gcggccaaga atttcataat ggaattgatc ttgtaaatgg
    20701 aaatcctaat acacctgttt ttgcagcatt agatggggaa gttgttcagg ctggtgctaa
    20761 ttattatgac tggtatggca attacgtggt tattaaacat aataatggga agtggacagg
    20821 ttatgctcat ttgtctcgta ttgatgtttc tgttggacaa aaagtccaga aaggtgctca
    20881 aataggcttg atgggaacaa ctggtccatc tacaggagaa catctacatt ttcaaattat
    20941 gaaaaattat tggccacagc cagttgttga ttttgagaat ccaagaaatt atatccaatt
    21001 ttaaggtggt gattctatga gtaaatggaa tgtcgtttta agtacaacag aaccatataa
    21061 ttatgtgggg atgattcaag ttcgacaagg caataagaat acagaggtta tggaagcgac
    21121 tatagttgaa aatggtcttc cctacgattt atcagaatgt aaggtatatt ttgaatcagt
    21181 tgtaggtggg aaatatccag tccaattaga aacaaaaatt gtggatgcta aaaaaggaaa
    21241 aattaactat atttttgata aatattccat gcagtgttta catcgacaaa cagccaattt
    21301 cattatattt aaaggagaag acttgattgg aacaactcaa gacttctctt attttgtcat
    21361 taatgctgtt tcaaaaacag aaggagaaat gggttcttat tggcaatcaa tcgaagattt
    21421 aattgcggat atgacggact ttattaatga aaataaaggc gattttacgg actggatgaa
    21481 tgaaaggaaa gaagaatttg aacgttggag acaagaacag gaacacagtt tccaagattg
    21541 gagagaagga caagaatcag actatttggc atggtttgaa tcaatcaagg atattttaaa
    21601 gacgattgat ccaggtggag tcatgttagc cgaactaacg gatgcgcgag tagatattca
    21661 aggagttcgt catgcgtcta tttcagagcg tttgctggca gatatggact atttatatca
    21721 gaaattgcga gcaacgcttt tcacgattga atacggggag attgaagtga ctgatatttt
    21781 gcaggatgat ctcttttcag ataatcatga agtcgaaaaa gttggaactg tagcattccc
    21841 gattgaagaa ggagccttga tcatcgcaac cgttgatgat ccaaagcaaa atgtgttcac
    21901 tcttgagaaa gttggggtga tctaatggct aaaactaaac gaatgatgga aacggatgaa
    21961 aaaacgggtg tacaacgcca attctttcca atcacacatg cttccgcagt tcttgggtta
    22021 gaagaaataa tagcaggaga agcaacagtt ttatctgtta atggaaaaat tggagccgtc
    22081 gtcattacta aagaagattt gggattagag aatgttctca cagaattacc ctatgcaagt
    22141 gaagaagatg acggcattat cacggcagaa atgtatcaaa aaattttaaa cagtggagaa
    22201 ggtgactacg tgttgccagt tgccactatc gaccgtttgg gtggcataaa agttggtgaa
    22261 ttattgacga ttgatgagac agggaaagtc tctgcagtca gacaatctga tgtcaatttt
    22321 tcgctggagt taaaagaaaa actcgattca ctgaaaaatt atactgctgg agaaaacatc
    22381 actattgatg aagatggaac aattagttct acaggtggta gtgggactgg tggtgtcaat
    22441 cagtcatatg tagatcaaaa attccaagaa gctgtaaatc aagcagaaaa ttacacaaac
    22501 gaaagaattc caaattttac ttttgaaaaa attggggagg tatagagaat gacagatatc
    22561 gtaaaaataa aacaaagcaa tgtacaggtt tatccacaaa ctcattggaa tgctatagaa
    22621 ggcaaaccaa caacggtgaa gggagataaa ggcgatcctg gccaagcagc aacaattacc
    22681 ataggaaccg tttctagtgg ttcaaccgct tctgttacga atgtaggaac atcatctgca
    22741 gctagattta attttgtttt accaaaggga gataaaggtg atcctggaat aaatgccaca
    22801 actacagctg tagctacaac tacagcaaat gggttgatgt cgtccacaga taaaactaag
    22861 ttggatggaa ttgcagctgg agcacagaaa aatccaggta atgctacaac tacgacagca
    22921 ggtttaatgt cagcaaccga taaagtgaaa cttgatggat tagccaatat tacatttgag
    22981 aaagtgggga cggtttaatg gctgatattg ttcaattaaa agaagacgga gttgctaaat
    23041 acttaaaaac gcatgtaaaa gctattgatg gtaaagaagc attggtgcag acagatggag
    23101 accaagccat tgcaggacat aagaattttt caggttctgt aacaatcaat aataaacgtg
    23161 ttttgacgac tgatgataac agatatgaag tggtaaatct ggttgtcact aacggtaata
    23221 caggg
//
LOCUS       DAHHZJ010000030        23106 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.031, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000030 DAHHZJ010000000
VERSION     DAHHZJ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.031"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="NBL02_000948"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="NBL02_000948"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCD2571653.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="NBL02_000949"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="NBL02_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCD2571654.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="NBL02_000950"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="NBL02_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCD2571655.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="NBL02_000951"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="NBL02_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCD2571656.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="NBL02_000952"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="NBL02_000952"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCD2571657.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="NBL02_000953"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="NBL02_000953"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCD2571658.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="NBL02_000954"
     CDS             complement(8053..8523)
                     /locus_tag="NBL02_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HCD2571659.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="NBL02_000955"
     CDS             complement(8569..8994)
                     /locus_tag="NBL02_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HCD2571660.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="NBL02_000956"
     CDS             complement(9061..9330)
                     /locus_tag="NBL02_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HCD2571661.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="NBL02_000957"
     CDS             complement(9341..10936)
                     /locus_tag="NBL02_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCD2571662.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="NBL02_000958"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="NBL02_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCD2571663.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="NBL02_000959"
     CDS             complement(14489..15049)
                     /locus_tag="NBL02_000959"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCD2571664.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="NBL02_000960"
     CDS             15402..16376
                     /locus_tag="NBL02_000960"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCD2571665.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="NBL02_000961"
     CDS             16546..17946
                     /locus_tag="NBL02_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HCD2571666.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="NBL02_000962"
     CDS             complement(18024..18746)
                     /locus_tag="NBL02_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCD2571667.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="NBL02_000963"
     CDS             complement(18825..19331)
                     /locus_tag="NBL02_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HCD2571668.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="NBL02_000964"
     CDS             19454..21253
                     /locus_tag="NBL02_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HCD2571669.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="NBL02_000965"
     CDS             complement(21321..22895)
                     /locus_tag="NBL02_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCD2571670.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT         6328 a         5014 c         4223 g         7541 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggttgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataa
//
LOCUS       DAHHZJ010000031        22650 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.032, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000031 DAHHZJ010000000
VERSION     DAHHZJ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.032"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            4..816
                     /locus_tag="NBL02_000966"
     CDS             4..816
                     /locus_tag="NBL02_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HCD2571671.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="NBL02_000967"
     CDS             813..1613
                     /locus_tag="NBL02_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCD2571672.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="NBL02_000968"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="NBL02_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HCD2571673.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="NBL02_000969"
     CDS             3110..4819
                     /locus_tag="NBL02_000969"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCD2571674.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9383
                     /locus_tag="NBL02_000970"
     CDS             5031..9383
                     /locus_tag="NBL02_000970"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HCD2571675.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9527..10000
                     /gene="rimP"
                     /locus_tag="NBL02_000971"
     CDS             9527..10000
                     /gene="rimP"
                     /locus_tag="NBL02_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCD2571676.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10024..11199
                     /gene="nusA"
                     /locus_tag="NBL02_000972"
     CDS             10024..11199
                     /gene="nusA"
                     /locus_tag="NBL02_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCD2571677.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNSKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11221..11514
                     /locus_tag="NBL02_000973"
     CDS             11221..11514
                     /locus_tag="NBL02_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HCD2571678.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11511..11822
                     /locus_tag="NBL02_000974"
     CDS             11511..11822
                     /locus_tag="NBL02_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HCD2571679.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11835..14141
                     /gene="infB"
                     /locus_tag="NBL02_000975"
     CDS             11835..14141
                     /gene="infB"
                     /locus_tag="NBL02_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCD2571680.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14168..14515
                     /gene="rbfA"
                     /locus_tag="NBL02_000976"
     CDS             14168..14515
                     /gene="rbfA"
                     /locus_tag="NBL02_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCD2571681.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14623..14844)
                     /locus_tag="NBL02_000977"
     CDS             complement(14623..14844)
                     /locus_tag="NBL02_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCD2571682.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            15292..17655
                     /locus_tag="NBL02_000978"
     CDS             15292..17655
                     /locus_tag="NBL02_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HCD2571683.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKLGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRNDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFHSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAKKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            18023..18946
                     /gene="truB"
                     /locus_tag="NBL02_000979"
     CDS             18023..18946
                     /gene="truB"
                     /locus_tag="NBL02_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCD2571684.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18950..19891
                     /gene="ribF"
                     /locus_tag="NBL02_000980"
     CDS             18950..19891
                     /gene="ribF"
                     /locus_tag="NBL02_000980"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HCD2571685.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            20243..21028
                     /locus_tag="NBL02_000981"
     CDS             20243..21028
                     /locus_tag="NBL02_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCD2571686.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            21041..21892
                     /locus_tag="NBL02_000982"
     CDS             21041..21892
                     /locus_tag="NBL02_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HCD2571687.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(22016..22468)
                     /locus_tag="NBL02_000983"
     CDS             complement(22016..22468)
                     /locus_tag="NBL02_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD2571688.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
BASE COUNT         7690 a         3950 c         4953 g         6057 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgtgtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccagctagga cgaaatattc
     5701 cagccgatga accaacgaca ccaatgatca gtatcgttga agaagaacga agagtcacac
     5761 ttgaggggta cgtattcgac aaagaagtca gagaactacg ttcaaaacgc aagatcttga
     5821 ctttgaaaat cacagattat acttcatcat ttatcgtcaa aaaattctcg aacggcgaaa
     5881 aagatgaaca agtatttgat gcgatcagtg tcggtagctg gttaaaggta cgaggaagta
     5941 ttcaagaaga tacgtttgtc cgtgatttgg tcatgaatgc gcaagatatc atagaagtca
     6001 aacatacacc tcgcaaagat tatgctcctg aaggggaaaa acgagtagag cttcatgttc
     6061 atagcaatat gagtacgatg gatgcaacaa acagcatctc tgatcttgtg gcacaagcag
     6121 gaaaatgggg acaccgagct atcgccatta cagaccatgg tggtgcgcaa gcctttccgg
     6181 aagcacatag tgctggaaaa aaagcaggtg tgaaaatctt atacggcgtg gaagcgaatg
     6241 tcgtagatga tggtgtccca attgcttata atgatgcaca tgaagcattg agtgaagcca
     6301 catacgtcgt atttgacgtg gaaacgacag gattgtctgc agtatatgac acgatcatcg
     6361 agctagccgc agtcaaaatg tataaaggaa atgtgatcga aagctttgat gaatttatcg
     6421 atccaggaca tccattgtca cgaacaacgg tagatctgac tggtatcacg gatgaaatgg
     6481 ttcgagggtc aaagtcggaa gaagaagtat tgcggatgtt ccttgaattt tcaaaagata
     6541 caattttggt agcccacaac gcggcttttg atatggggtt cttgaatact agctacgcaa
     6601 ggtatggtat tcctgaagcg gccaatccag tcattgatac gttggaatta gctagatatc
     6661 tgtatcctca attcaaacgt ttcggattag gtgtgttatc taaaaagttt ggtgttagct
     6721 tggaacagca tcaccgagca atctatgatg ctgaagcaac tggtcactta gcatggatct
     6781 ttgtgaaaga ggcaatggac aatcataaca tgctttatca tgatcagcta aatgagcata
     6841 tcggagaagg agattcttac aaaagggctc gacctttcca tgtaacgatt ttagcaaaaa
     6901 atcaggcagg actgaaagat ttgttcaaac tgatttcaat gtcgaatgtc gaatacttcg
     6961 aacgcgtccc acgaatacca cgctcccaat tgaaaaagat gcgtgaaaat ctattgatcg
     7021 gctcagcttg tgataaggga gaaatattcg aagccatgat gcaaaaaggg gtagaagagg
     7081 caagaaaccg agcaaaattc tatgattata ttgaagttat gccaaaagcg gtctatgcgc
     7141 ctttgatcga gcaagaattg gtaaaaaatg aacatgattt ggaagaaatt atccaaaatt
     7201 tggtagaaat cggaaaaagt ttggataaaa tcgttgttgc aacaggtaat gtacattatc
     7261 tgaacgaaga agatgccatt taccgaaaaa ttttgatcaa ttctatgggc ggagcgaatc
     7321 cgttgaatcg tcatagtttg ccggatgtcc atttccgaac aaccgatgag atgctaactg
     7381 catttcactt tttaggggaa gaaacagcga aagagatcgt agtagaaaat acgaataaaa
     7441 tcgcagatat ctgcgaagaa gtgattccag tcaaagatga actttataca ccgaagattc
     7501 caggttcaga agatgaaata tcagaattga gttacacaaa agcaaaacaa atgtacggag
     7561 atccgttacc tgagatcatt caaaaacgat tagaaaaaga gttgaattct attaatggta
     7621 atggcttctc ggttatttat ctgatcgccc aaaaactagt gcataaaagt aatgaagatg
     7681 gctatctagt aggttcacgt ggctctgttg gctctagctt tgttgcgacg atgacaggaa
     7741 tcactgaagt taatccatta gcccctcatt attattgtcc agagtgccaa tattccgagt
     7801 tttttgaaga tggtacgtat ggttctggat ttgacatgcc agaaaaacaa tgtccaaaat
     7861 gtggtgcacg cttaaacaaa gacggtcatg atattccatt tgagacattt ttaggattcc
     7921 atggagacaa agtgcccgat attgatttga acttctcagg tgattatcaa gcagaagccc
     7981 ataactatac gaaagtattg tttggcgaag actatgttta ccgtgccgga acgattggta
     8041 cagtcgccga caaaacagca tatggctatg taaaaggata cgaaagggac aataatctgc
     8101 agtttcgaag tgcagaagtc gacagattag caaaaggcgc tactggagtc aaacggacaa
     8161 ccggccagca tccagggggg atcatcgtta ttcccgatta tatggatgtc tatgatttta
     8221 cgccgatcca atatccagca gacgatcaaa attcagaatg gaaaacgact cattttgatt
     8281 tccactcgat tcacgataat gtattgaaat tagatattct agggcatgat gacccaactg
     8341 ttattcggat gcttcaagat ttatcaggga tcgacccaca aacgatccct acggatgacc
     8401 cggaagtgat gcggatattt gccggtcctg aagtacttgg agtaagtcag gaacaaattt
     8461 attccaaaac agggacatta ggtattccag aattcggcac tcgttttgtt cgagggatgt
     8521 tagaagaaac gcatcctaca acttttgcag aactactgca aatttccgga ctatctcatg
     8581 gtacagacgt atggttagga aatgcggaag aattgattcg acgaggagat gcgacactgg
     8641 cggaagtaat cggctgtcgt gatgatatta tggtttattt gatccatgct ggattagata
     8701 gcgggatggc atttaaaatc atggaaactg ttcgtaaagg acaatggaac aagatccctg
     8761 atgaattgcg agaaacctat ctatcggcga tgaaagaaaa taatgtgccc gactggtata
     8821 tcgattcttg ttcaaaaatc aaatatatgt ttccgaaagc ccatgctgca gcttacgtat
     8881 tgatggctct tcgtgtcgct tatttcaagg tttatttccc tattttgtat tactgtgcat
     8941 acttttctgt acgtgcagac gactttgatc tagtctccat gtgcaaagga aaagatgctg
     9001 tgaaacaagc aatgaaagaa atcacggaca agggattaga tgcttccgta aaagaaaaga
     9061 atcaactgac agtgctggag ttagccaatg aaatgctgga acgaggattc aaatttggaa
     9121 tgattgattt gtataaatcg gatgccgtta actttgttat tgaaggtgat acgttgatcg
     9181 caccatttag agctgtccca agtttgggaa ccaacgtggc gaaacaaatc gttgaagcaa
     9241 gaaaagacgg tccattttta tccaaagaag atcttgccac ccgcggtaaa gtttcaaaaa
     9301 cattgatcga atatatgaac gacaatggcg tattgaaaga tctaccagat gaaaatcaat
     9361 tatcgttatt cgatatgcta taagcatctg ttttcctgtc tgcttgtaat tttaagcaga
     9421 gtatggtata gttcttatag gtaatgatac taactgtgag tgagcggatt tttcgctcac
     9481 tctttttaat ggaattacgc acttaatttt taaggaggcg aaacttttga gtagcgtcgt
     9541 tgaaacagtc acagaaatgg tgacaccgat tttagatgaa caaaattttg aacttgtcga
     9601 agtcgaattt gtaaaagaag gaaaaagctg gttcttacga gtatttattg acaaagaagg
     9661 cggtatcgat atcgaagaat gcgcctttgt cagtgagaaa ctgagtgaaa aacttgatgc
     9721 agcagatcca gatccgattc ctcaagctta tttcctggag gtatcctccc cgggagcaga
     9781 gcggccattg aaaaaagaaa gcgactatga aaaggcactt ggtgattatg tccatgtctc
     9841 cttataccaa ccagtagatg gcgaaaaaca atatgaagga tttcttcaat catttgatgc
     9901 tgaacagctt acattgaaaa tacgcataaa aacacgagaa aaagaattga cttttgaccg
     9961 aaaaaatatt gcaaaagctc gcttagctat ccaattttaa tcacggagag gaatcagaaa
    10021 aaaatgagta aagaaatgtt aaatgcgtta gacgctttag aagctgaaaa agggatctca
    10081 aaagaaatcg tgatcgatgc attagaagca gcattggttt ctgcatacaa acgtcactat
    10141 ggccaagcac aaaacgtcga agtggaattt gatcagaaaa aaggaaagat ccatgtctat
    10201 gcggtaaaag aagtaacgga agaagtgatg gattcacagt tggaagtctc attgaaagat
    10261 gcacttttaa tcaatccagc atatgagatc ggtgatacga tccgttttga agtaacacca
    10321 aaagattttg gacggatcgc tgcgcaaacg gctaaacaag ttattttaca gcgtgtacgt
    10381 gaagcagaaa gaacgattat ttataacgaa ttcagtgctt atgaaaaaga catcatgcaa
    10441 ggaatcgtcg aacgccagga taaacgttat atttatgtca atcttggtaa aattgaggca
    10501 gttctttcta aacaagatca aatgccaaat gaattttatc aaccacatga tcgaatcaaa
    10561 gtttatgttt cccgagtaga aaatacgtct aaaggtccgc aagtatttgt tagccgcagc
    10621 catccagacc tattgcgtcg cttatttgaa caagaagtac cagaagtcta tgatggaatc
    10681 gttgaaattg tcagcatcgc ccgtgaagct ggcgatcgtt caaaagtggc agttcgctca
    10741 aatgatccaa atatcgatgc agtcggtaca tgtgtcgggc caaagggaca acgagttcaa
    10801 gcaatcgtca atgaattaaa aggcgaaaac atggatatcg tcgaatggaa tgaagatcca
    10861 gctgtattca tcgcaaatgc attgaatcct gcacaagtca tggatgtgat cttcgatgag
    10921 acaaattcaa aggcctgcac agttgtcgtt ccagactacc aattatcgct agcaatcggt
    10981 aaacgcggac aaaatgcacg tttagctgct aaattgacta accataaaat cgatatcaaa
    11041 tcagaatctg acatgacgga attttatgaa aatcaagaac aacaaaaaga aacagaagaa
    11101 ctgcatgatg aagcaatcgt tcagtcagat ttaacagacg atgaatatga aacaattgca
    11161 ttcaacaatg aaacggtcga agaaaaacca gaagcttaac tttagtaagg aggcaaagag
    11221 atgaaacaaa ggaaaattcc tttacgcaaa tccgtggttt caggtgaaat gaaacctaaa
    11281 aaagagatgg tccgaatcac ccgttcaaaa gaaggggaag tatcgatcga tccaactgga
    11341 aaaatgcctg gacgaggagc gtatgtctca cttgagccgg aagaagtaca acaagcttgg
    11401 gataaaaaaa ttctcgatcg agtactcgaa acaacattaa cagatgagtt ttatcaagaa
    11461 cttcttgact atgtcactca ccaaaaagca cgtaaagagt tatttggaaa atgaatacag
    11521 ttaatcggca aaaagccatg aatctcatcg gtctagcgat gcgcgcggga aaactaatca
    11581 cgggtgaaga actgaccatt gctgaaatcc gcaaacaaaa agcaaaattg gtgtttgtgg
    11641 caaccgatgc aagtgagaat acaagaaaaa agataaaaga taagagttca tattatgaag
    11701 ttccttgctt tgagctattt tctgaagcag aaatcacgca gatgatcgga aaacctcgca
    11761 aggtattcgg aataatggat gccggatttg cgaagaagac caaggagcta attgaaggtt
    11821 aggaaggtga ttgcatgggc aacaaaagaa tctatgaatt agcaaaagaa ctcaaaaaat
    11881 ctagtaaaga cgtcgtggaa aaagcacaac aacttggcat cgatgtgaaa aaccatatgg
    11941 gtgcgattac tagcagtgat gagaagaaat tgcaacaagc gttcaaaaac ccaagcacga
    12001 aacaacaagc tgcacaacaa gcaagcagaa aaccagccaa tcaacagccg aacgatcaac
    12061 gtaaaaaaga aacaacgaac aatcaacaaa aaaataaaaa taaccgcagt tatcaagatc
    12121 gcggtcaagg gagcggtcaa gtgaatcaag gaaaaaaacc aacaacaaac caaaataata
    12181 cgggaaatac cagcagccaa aatcgtcaag gaacgagcca aggcggcaat caaaaccgta
    12241 ctaacaataa caacaaacca aacaataaca atcgcggcaa atttaataat aataaccgta
    12301 atcgttataa taaaaaaggt aaaaaaggca aacagcaaca atcaactaaa ccagctgtac
    12361 caccacgcaa attccgtgaa ttgccagatg tattagagta tactgaaggt atgaacgtag
    12421 cagatattgc gaaaaaaatt catcgtgagc cagcagaaat cattaaaaaa ttatttatgc
    12481 ttggtgtcat ggtcaatcaa aaccaagcac tggataaaga tacgattgaa ctgttagcga
    12541 cggactatgg catggaacca caagaaaaag tccaagttga cattgctgat atcgataaat
    12601 tcttcgaggc agaagaagtc gatccagata aattagtctc acgtccgcca gtagtaacga
    12661 ttatgggaca cgtagaccac gggaaaacaa ccttattgga tacattgcgc cattcacgtg
    12721 taacaagcgg cgaagccggc ggaatcacac agcatatcgg tgcctaccag atcgatattg
    12781 atggtaaacc aatcactttc ttggatacac caggacatgc ggcctttaca agtatgcgtg
    12841 cacgtggagc aagtatcaca gatatcacga tcttagttgt tgctgcagat gatggcgtca
    12901 tgccacaaac agttgaagca atcaaccatg ccaaagctgc aggcgttcca atcatcgtcg
    12961 ctgtaaataa aattgataaa ccaggtgcaa atcctcagca tgttatgcaa gaattaagtg
    13021 agtatgaatt aattcctgaa gcatggggtg gagaaacgat ttttgttgag atctcagcga
    13081 aattcggcca aaacatcgaa gaattgctgg aaatgatcct gttagtcgca gaagtagaag
    13141 atttaaaagc agatccaaca caacgcgcaa tcggaacagt gatcgaagca cgtcttgata
    13201 aaggtaaagg tcctgtgtca accttacttg tccaagaagg aacacttcac gttggtgacc
    13261 caatcgttgt cggtaatact tacggacgtg tccgtgtaat gacaaacgat cttggccgtc
    13321 gcgataaaga agcaggacct gcaacgccag tagaaatcac tggcttgaat gatgtacctc
    13381 aagcaggtga ccgtttcgta gtcttcgaag acgaaaagac agcacgtgca gcgggtgaag
    13441 aacgcgcaaa acgtgcatta ttggaaaacc gtgctgccac aagccgtgtg actttggaca
    13501 acttatttga aagcctgaaa gaaggcgaat tgaaagaagt taacgtaatc atcaaagcag
    13561 atgtacaagg ttcagcagaa gcactggctg catcattgaa gaagattgat gtggaaggtg
    13621 tccgtgtaaa aatcgtccat tcagctgtcg gagcaatcaa tgaaagtgat gtaaccttag
    13681 cagcagccag caacgctatt atcatcggtt tcaacgttcg tcctacaccg caggcaaaag
    13741 tgcaagctga tactgaagaa gtcgatattc gtcttcaccg tatcatctat aaagccatcg
    13801 aagaaatcga aactgcgatg aaagggatgc tagaccctga atttgaagaa aaaatcaccg
    13861 gtcaaatgat cgttcgtgaa accttcaaag tttctaaagt tggtacgatt gcaggtgcct
    13921 atgtaacaga aggatatatt cgtagagata gcggtgtccg tgtaatccgt gacggaatcg
    13981 taatctatga aggccaactt gcaagtctga aacgattcaa agatgacgtg aaagaagtca
    14041 aaatgggtta tgaatgtggt gcgatgattg aaaaattcaa tgacatcaaa gtagatgacg
    14101 taattgaagg atttatcatg gaagaagtca aaaatgacta aaaactgata aaaggaggcc
    14161 aatcattatg gctaactacc gtgatcgccg agtcggtcaa gaaatcttaa aagaagtcaa
    14221 tgatattttg cgcaaaaagg tccgtgaccc tcgtgtggag aacgtaacga tcacagatgt
    14281 gaaagttacg ggggacttac agcaagcaac gatctattac agcattttat ctgacttagc
    14341 atcagataag aaaaaagcac aagatggatt gaataaagca tcaggattga ttcgtcgaga
    14401 gttagggcac aatctaagta tttataagac acctgaattg ttctttgaac ttgatgaatc
    14461 agttgtctat gggaaccata tcgatgaatt gcttcgcgac ttgaacaaag actaatcaaa
    14521 aaatcccgca aatcctgtga aatcaggggt tgcgggattt ttttgttttc attaagacgt
    14581 attacctaag agttccatga ctttagtctg gtagcgtgtc agctaaccaa aaaagagaaa
    14641 aagaaaagta aaaaattcga ctttcttttt tctatagtat ataaattttt gcatatagat
    14701 aaagatcaag ataccgataa cggaagctag attgtaatct attttagtga cagtcaggta
    14761 tagccaagta actagaagat cagtggaacc tgcaccataa aagtttgagt ttgtcggtcc
    14821 gttgacagtt aaaagaaaaa tcacgttgaa gttattgata tttccgatta cgccgcttcg
    14881 atttggtcac gatccaaatt ttggataatc cctgtagaaa ttagcatagt agctgggata
    14941 ccaattcaca tattgacaaa gataacagag acttttacat aaaacggatc aatcaggaaa
    15001 ggaattgctc gatccagccc cagttttcca acaatgtgtt aactggccta gcgtcattca
    15061 gcaaattatg catgatgagg agcgaaatga actgcggaac agccattgtg ataacgacaa
    15121 gttttttgga tcaatgtaaa aaacgtcagc gaaaggaaaa atttcgtggt tttttatacc
    15181 tgttcatttg gctagatata aaatccaaac aaatgaacag aagtgcttat taggtttcaa
    15241 ttgggagtat ctgcgctaga ataagagtaa taatgaaaag gagtgttatg tatggattat
    15301 tctagtaaag aatactttga taaaatgacg gcttggtggc gtgctgctaa ttatctttct
    15361 gttgggcaac tttatcttaa agataacccc ttactgagac gtacactgaa accagaagat
    15421 gtcaaaaaac atccaatcgg tcattgggga acgattcctg gccaaaattt tatttatgtc
    15481 catttgaacc gagtcatcaa taaatatgat ttgaacatgt tttatatcga agggccagga
    15541 cacggcggac aagtcatggt atcgaatgct tatttagatg gaagttacac agagatctat
    15601 ccagaagtaa cggaagatga gacaggaatg caaaaattat tcaagcgatt ttcatttcct
    15661 ggaggcattg cttctcatgc agcaccagaa acaccaggtt ctatccatga aggcggagag
    15721 cttggatact ctctttcaca tgcagtcgga gctgtattag acaatcctga agtaatctct
    15781 gctgtcgtga ttggtgatgg tgaagcagaa accggtcctt tagctggaag ctggttctca
    15841 aatgtcttta tcaatccagt gatagatgga gctgttttac cgatccttca tttaaatggt
    15901 gcaaaaatag ccaacccaac gatcttggca cgtaaatcag atggagaatt agcaaattac
    15961 tttaacggac ttggctggga gcctttcttt attgaaggaa atgatccaga aaaattaaat
    16021 cctgttatgg ctgaaaaaat ggatcaagca atcgagaaaa tcaaatccat ccaaaaagaa
    16081 gctcgcctga agacagcaac agatgtcgtg atgccaaaat ggccagtatt gatcgtacgc
    16141 acaccaaaag gatggaccgg tccaaaagaa tgggacgggg aaccaattga aggaaccttc
    16201 cgagcacacc aagtaccgat tcctgtagat caagaacata tggatcatgc agatgcttta
    16261 ctccgttggc tgaaatccta tgaaccagaa aaattatttg atgcacaagg acgtatttta
    16321 gaagaaatca gagaaattgc gcctactggt gaccaaagaa tggcaaaaaa tcctatcacc
    16381 aatggtggaa tcgatccaag accgcttatt atgccagatt ggaaaaaata tactttgcaa
    16441 tttgaaaaac taggctccgt taaagcagaa gatatgaccg aacttggtaa attcgttcgc
    16501 gagatcattg agaaaaatcc agaaaatttc cgtatatttg gaccagatga aacaaaatca
    16561 aaccgattga atcaagtttt caaaacgaca aatcgccaat ggatggaaaa aatcgaacca
    16621 gaaaatgatg aatggctttc gccatctggt cgcgtcatcg acagtcaatt atcggaacac
    16681 caagatgaag ggttcttaga agggtacgtt ctgactggac ggcatggttt ttttgcaagt
    16741 tacgaatcat tcttgcgagt ggttgactct atgttgactc agcacttcaa atggatacgg
    16801 aaatcacatg acctttcttg gcgaaatgat tatccttcgc taaatctcat tgcaagctca
    16861 acagtcttcc agcaagatca caatggatat agccaccaag atccaggaat attgacacat
    16921 ttggcagaaa agaaggcgga gttcatccgt gaatatctgc ctgcggatgc aaatacattg
    16981 ctcgcagtca tggataaagc attccactcg tcagaaaaaa tcaatttgat catctcttcc
    17041 aaacatccgc gtgctcaatt ttactcagca gaagaagcag ccgtcttagt gaatgaaggc
    17101 ttgaaaatca ttgattgggc ttcgacagca aaagaggagg aacctgaatt ggtcattgct
    17161 gctgcaggaa cggaatctaa cttagaagca ttagcagctg taactttgct gctggaagaa
    17221 ttcccaaaac taaaaattcg gttcatcaat gtcgtagatt tattgaaatt acgtcatcca
    17281 agtcaagatc cgcgtgggtt gagcgatgaa gaatttgatc aatattttac aaaagacaaa
    17341 ccgatcttat ttgctttcca cggttatgaa acattggtcc gcacgatttt ctttgatcgt
    17401 cataaccatc atttaatgat tcatggctac aaagaaaatg gagatatcac gacaccattt
    17461 gacatgcgtg tagtaaatga acttgatcgt tatcatttgg caaaagacgc agctttaaaa
    17521 atcaaaggaa gtcaagcaga agattttgcg aaaaaaatgg atcagaaatt acaagaacat
    17581 caaaactata ttcgtgaaaa tggtattgat ctgccagaag tattggattg gaaatggaaa
    17641 aatcttgacc aataaaatga taaaatcgat cccgcaaaat agaatttttt acattgatta
    17701 agaaactgaa aaaataaata aaaaaaacgg aaaaaagact ggaagaatgg ccagtctttt
    17761 ttttcgtttt tgggaagtga aaaccaaact atttacctgt aagccaccca taaatccaat
    17821 caaatggtgc aaagaaaaaa tcttccattc tattccctcc tttaaaaaag cataacatga
    17881 tttctattta cgtgagcatg aattttgtta cgtttttatt gccgaaaaat aaaaaccgtt
    17941 cattatgatg tcaaattcgt cagtaattga tgcaatgtgt tataataatt cagttaaatc
    18001 aaaaatgaaa tggagaaaag acatggacgg gttattacct ttatggaaag aacgcggaat
    18061 gacaagtcat gattgcgtat tcaaattaag aaaaatctta catacgaaaa aaatcggaca
    18121 tgggggtacg cttgacccag acgtagacgg tgtgttgccc atttgtattg gaaaagccac
    18181 aaaagttatc gaatatttga cagattctgg gaaaatctat gtaggcgaga taactctagg
    18241 tttcagtaca acgacggaag atcgatccgg agaacgggta gaaacaaaac cagtagatat
    18301 gtctttatct gaaaaaaaaa tagatgaagt gatggcttca tttattggtg agatcaccca
    18361 gataccacca atgtactcag cggtaaaagt caacggtaag cggttatatg aatatgcccg
    18421 caataatgaa acagtggagc gtcctgttag aaaggctcag atttaccgtt ttatccggac
    18481 aagtgaattg acaagagacg aagtgacagg aaccatttct tggcgcttcg aagtagaatg
    18541 cgggaaagga acctatgtcc gcacattagc tgtagataca ggcaagaaac ttggttaccc
    18601 tgcgcacatg tctgatctga cccgaacagc aagtgccggt atgtcccaga aggatgctct
    18661 gacattagaa gaagtagctc aagctgtgaa aaatgggaca atcgatgatt gtttgctgcc
    18721 aatagaaaaa ggagtggaac ggttcaaacg agtagatatt actgatgaag tatggcagaa
    18781 agtaaaaaac ggtatgagac ttgattatac tgtatttgcg ctcccagaaa tgccgtcaga
    18841 agaaattgct ttgttttata aaggaaaagt tgtcagtatt tatcagccaa acccgaaaga
    18901 aaaaaacaaa ttaaaaccta gtaaagtatt gagaaatgag gaataacgaa tgaatatcat
    18961 caagatcaga catccttacc aatcagaaca aattccagaa gacgatgtcg ttttagtgct
    19021 gggatttttc gacggcgtgc atatcggaca ccagaaagtc ataaagacag gtatggaaat
    19081 tgcacaaaaa gaaggattga aattagcatt gatgactttc aatcagcatc catctattgt
    19141 ctttaaaaag atcgatccta gcagcgtgaa atatttgact agccttaagc aaaaagaaga
    19201 aaaaatggcc gctttgggta tagattattt atatgaagtt gattttactt cttcttttgc
    19261 tcatttagca ccgcaggaat ttgttgatca atacatcgta gggttacatg caaaatatgc
    19321 tgtttccggt tttgattata cgtatggacc aaaagacatt gcagatgtcg ctcattttcc
    19381 tgaatatgcc aaaggaagat tcaaaatcgt cacagtttct aaagaagaag aacaaggtga
    19441 aaaaatcagc tctacccgta ttcgtggatt actagacagc ggacaagtaa aagaagcagc
    19501 aaaactatta ggttgcacat atgaggtaga aggggtagtt gtccatggcg atgcacgagg
    19561 cagactacta ggattcccta cagcaaatgt caaagtaaag agcacggttc atttgcctaa
    19621 agaaggtgtt tatgtgtcgg aaataaaaat tggggaaaca tggtatccag ccatgggttc
    19681 aattggacat aatgatacat tcggtgaagg acgggagctg actgtagagt tgtatatttt
    19741 ggactttaat caagatatct atggtgaaca tgtcgcggta cgctggaatc aatttttaag
    19801 agatcaagtg aaattttcta gtgtagagga attgattgaa caactcaagg aggatgaaca
    19861 agcaacagct gaatattttt ctcagcaata aaacattatg cataaaatct cttgtttatg
    19921 tataactagc aattacaata aaatgggtga taaataagcc caggaaaggg gctgtgacaa
    19981 aagtcttgtc acagcctttt tttccaaata aatggttttt cagaagtagc ttcttcggaa
    20041 ataagccgaa atttcttgaa gctgaccact tctgtcacaa cctctttctt tatagaaata
    20101 aaaggacact agcaattcat gaaaacgatt gctagagcgt atcatttgct ttagactata
    20161 tatcttggtg tacactcact ttaaggcgga aataaaagtt atccgtaata acaggtttag
    20221 gtatgaaaaa ggagtgggta ctatgtacaa ggaattagaa aacaaagtcg cagtagtaac
    20281 tggtggctca aaggggattg gaacagcgat tgctcaacga ttcgggaaag aaaaaatgaa
    20341 tgtagttgtc aactataact cggataaaga aggagcagaa aaagcagtca aagcaatcga
    20401 agaggcaggc ggaaaagcag tctctgtaca agcagatgtc agttctgaag aaggaataca
    20461 gcgtttactt gatacggctt tagaaaattt tgggacactg gacgtttggg ttaataatgc
    20521 tgggatggaa aatcagcatc caacccatga acttagcctt gaagattggg aaagagtgtt
    20581 aaaagtcaat ttgactggcg tttttatcgg aacgaaagcg gctttaaatt actttacaga
    20641 gaataataaa aaaggaaata tcatcaatat gtcttctgtt catgaacaga tcccatggcc
    20701 aacgtttgct cattacgctg cttcaaaagg cggggtaaaa ttatttaccg aaacggtagc
    20761 aatggagtat gcaccgaagg gaatcagaat caatagtata ggccctggtg ctatccgtac
    20821 acctattaat gcggcaaaat ttgatgatcc tgaacaaaaa gaactcttgg aaagtatgat
    20881 cccactagga cgcgtaggtg aaccaaaaga aatagcggcg acagctgcat ggttggcttc
    20941 agacgaatcc agttatgtta caggtattac actatttgca gatggtggga tgactttata
    21001 tccttctttc caaggtggaa aaggataagg gtgaataaaa atgggtttat tgattgcatt
    21061 gatcccggca gttggctggg ggatccagcc actgattttg aaaaagattg gtggcagacc
    21121 aacaaatgag atattaggaa ctggtattgg agcggtgctt gtcggcttac tcgttcaatt
    21181 attcgcttct ccagctggta tttcagtgac tacatttttg attagtttac tttctggtgc
    21241 attttgggtt attggacaaa caggacagta tacatctttc aacttgattg gtgtttctaa
    21301 aacgatgcca atttcgactg cattacagtt agtcggaacc tcgttgattg gtgtcttcgc
    21361 ttttggagaa tggtctggaa gtatggggaa aatcattgga gcagcagcaa tcattcttct
    21421 tgtatttggt tctgctttaa cggcaatcag tgatgatcct ggaaaaaaag gcggtatcac
    21481 aaaaggaatt agtctattag catcaacaag tattggttat tgggtataca gtgcattgcc
    21541 taaactagtt gatgcaagcg gactttccat tttctttccg caaatgatcg gtgtcttttt
    21601 aggggcagtg atttatgtga ttttccgtaa accaaccgca ttttctgaag acaagagttg
    21661 gaaagcaacg atcgttggag ccatctttag tgtgtcagca ttggcttata ttttttcagc
    21721 aaatgaaaat ggggttgcta ctgcatatat cattacacag ctaaacgtag ttatctctac
    21781 attaggcggt atgtacatcc tgcatgaaaa taagacacca aaagaaaaaa aattaacgat
    21841 tatcggatta ttgctgatcg tagtaggaag catggtcacg gtctttattt gaggataaag
    21901 atggtaaaat ttcatttatt aaaaaaatat ttgtttgaac caaacagtaa gtacttataa
    21961 aaaaatagcg ctagaatttc caacatctct ggaatttcta gcgcttttta attgtttaag
    22021 atacaaggga aatatctgtc tggatcaata atttttcatt gatccattga ctaaagttta
    22081 cgacataatt gaagttacca ccgattagta tctccgcatg attgtcttta taaagatcgt
    22141 attgtaaaag acggtctttt ttatttgcgt aaacatcgtt atggtactgg actaattgtt
    22201 ctcggaattg attgattgct gtaaaatcac gatattgaag gttgaaacgc atcgtgattg
    22261 gtgcatttag catgaatcgc tgcatttctt ttgctgcttc ctctgcttga tctccgtata
    22321 ggaactcaaa aagattacgt tcagaaaatt tccattgccc gccaaccttt gttcctttta
    22381 gttttccttc atgaagataa tttctgatag aacgtgtagt aacacctaat tgttcggcta
    22441 attcatttac tgagtaaaaa tctttcaatg ttcaccctcc tatgtgaatg gttctactga
    22501 cttagttgat atttgcttaa tcacgaaatg tagaaagaac gattttaatt aaggtcattg
    22561 taaaaatcaa gtcagaaaat tctaactttt atttctctaa agaaattcgt tctctcctaa
    22621 agtgatatta tatttaaata tacctgaaaa
//
LOCUS       DAHHZJ010000032        22216 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.033, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000032 DAHHZJ010000000
VERSION     DAHHZJ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.033"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            32..817
                     /gene="purR"
                     /locus_tag="NBL02_000984"
     CDS             32..817
                     /gene="purR"
                     /locus_tag="NBL02_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HCD2571689.1"
                     /translation="MTQYLLNHPHELISLTSFAERYESAKSSISEDLGIIKKTFKERG
                     YGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGYVYLSDLLGEPE
                     LLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVRRDSKITEGSTV
                     SVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSLIEEFESELVGI
                     TVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1320..2693
                     /gene="glmU"
                     /locus_tag="NBL02_000985"
     CDS             1320..2693
                     /gene="glmU"
                     /locus_tag="NBL02_000985"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCD2571690.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2787..3758
                     /locus_tag="NBL02_000986"
     CDS             2787..3758
                     /locus_tag="NBL02_000986"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCD2571691.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3764..3866
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3932..4501
                     /gene="thiT"
                     /locus_tag="NBL02_000987"
     CDS             3932..4501
                     /gene="thiT"
                     /locus_tag="NBL02_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HCD2571692.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4582..5355)
                     /locus_tag="NBL02_000988"
     CDS             complement(4582..5355)
                     /locus_tag="NBL02_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571693.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            5622..7322
                     /locus_tag="NBL02_000989"
     CDS             5622..7322
                     /locus_tag="NBL02_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HCD2571694.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            7292..7510
                     /locus_tag="NBL02_000990"
     CDS             7292..7510
                     /locus_tag="NBL02_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HCD2571695.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            7530..8420
                     /locus_tag="NBL02_000991"
     CDS             7530..8420
                     /locus_tag="NBL02_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCD2571696.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            8438..8722
                     /locus_tag="NBL02_000992"
     CDS             8438..8722
                     /locus_tag="NBL02_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571697.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            9209..9823
                     /gene="gmk"
                     /locus_tag="NBL02_000993"
     CDS             9209..9823
                     /gene="gmk"
                     /locus_tag="NBL02_000993"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCD2571698.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            9828..10133
                     /locus_tag="NBL02_000994"
     CDS             9828..10133
                     /locus_tag="NBL02_000994"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCD2571699.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            10386..12794
                     /gene="priA"
                     /locus_tag="NBL02_000995"
     CDS             10386..12794
                     /gene="priA"
                     /locus_tag="NBL02_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCD2571700.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            12809..13300
                     /gene="def"
                     /locus_tag="NBL02_000996"
     CDS             12809..13300
                     /gene="def"
                     /locus_tag="NBL02_000996"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCD2571701.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            13293..14231
                     /locus_tag="NBL02_000997"
     CDS             13293..14231
                     /locus_tag="NBL02_000997"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCD2571702.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            14231..15589
                     /gene="rsmB"
                     /locus_tag="NBL02_000998"
     CDS             14231..15589
                     /gene="rsmB"
                     /locus_tag="NBL02_000998"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCD2571703.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            15602..16342
                     /locus_tag="NBL02_000999"
     CDS             15602..16342
                     /locus_tag="NBL02_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HCD2571704.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            16339..18408
                     /gene="pknB"
                     /locus_tag="NBL02_001000"
     CDS             16339..18408
                     /gene="pknB"
                     /locus_tag="NBL02_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HCD2571705.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            18573..19472
                     /gene="rsgA"
                     /locus_tag="NBL02_001001"
     CDS             18573..19472
                     /gene="rsgA"
                     /locus_tag="NBL02_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HCD2571706.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            19485..20135
                     /locus_tag="NBL02_001002"
     CDS             19485..20135
                     /locus_tag="NBL02_001002"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCD2571707.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            20136..20774
                     /locus_tag="NBL02_001003"
     CDS             20136..20774
                     /locus_tag="NBL02_001003"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HCD2571708.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            20929..21783
                     /gene="mreC"
                     /locus_tag="NBL02_001004"
     CDS             20929..21783
                     /gene="mreC"
                     /locus_tag="NBL02_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCD2571709.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            21787..>22216
                     /gene="mreD"
                     /locus_tag="NBL02_001005"
     CDS             21787..>22216
                     /gene="mreD"
                     /locus_tag="NBL02_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCD2571710.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRF"
BASE COUNT         7402 a         3997 c         4825 g         5992 t
ORIGIN      
        1 gaaagtacgt cgcagtgaac gtctagttga catgacacag tatttattga atcacccgca
       61 tgaattgatt tccttaacaa gctttgccga acgttatgaa tctgctaaat cttctatcag
      121 tgaagatttg ggaatcatca agaaaacatt taaagaacga gggtatggaa ccttggaaac
      181 aatcccgggt gctgcaggtg gtgtattgtt catccctgaa atttcatatg aagaggcgaa
      241 aaaatatatt gaatctctag cagaacgtct atcagaacaa gaccgtctgc ttccaggagg
      301 atatgtttat ctctctgatt tgttaggaga acctgaactg ctgcgtcaag ttggaagaat
      361 cattgcttct aaataccttg gtgaaaaaat cgatgctgta atgactgtag ccacaaaagg
      421 cgtacctatt gcccaagctg tgtcctatta tctgaatgcg ccattcgtta ttgtacgccg
      481 ggactcaaaa ataacagaag gatcaacagt aagtgtcaac tatgtgtctg gatcttctga
      541 aagaattgaa aagatggaac tgtctaagag aagtttgaaa cgtggttcac gtgtccttgt
      601 agtggatgat ttcatgaaag gcggcggaac agtcaacgga atgaagagct tgattgaaga
      661 attcgaatca gaacttgttg gcatcactgt atttgccgaa tcaaaattca acggtcatcg
      721 cgccatcgat gattatacgt cactgcttta tgtcaaagat gtagatactc aaacgaaaaa
      781 tattacggtt gtgccaggaa attatttttc agaataagag gttgtgaaaa agccgcttag
      841 tttcgagtag taagaaaaat tttgtgaaaa tggctttcca cattttttca taaatttggc
      901 ttaataccga gaaactggct tttgaacacc gtttatctag aggttgtgaa atcagcgtcc
      961 tgtcctgagt attagctcaa taaacggaaa aattgctcct catattttgc cgtttattga
     1021 gctaataccg aaggtcagct gattgaaaaa cgtttaatga agaggttgta agcctaccgt
     1081 ttaactctaa gtcacaagaa acgatttcaa aaaacagctt ttcatgtttt atgagtagtg
     1141 tgacttgtga ccgaggagtt ggcaggagag caccggttac caaatagata ggcccgttat
     1201 ggtctatcta tttttttatc cagaaaacgc tgggataaaa gaaaatcact tgttttgttg
     1261 aaaaaatggg tatactggga taaactgaaa tcagcaaact atatttagga gtgacgcagt
     1321 tggacgcacg ttatgcaatt attttggcgg cagggaaagg tacccgcatg aagtcaaaat
     1381 tatataaagt tttacatccc gtttctggta aacctatggt agaacatatc attaatcggg
     1441 tcagtgaaac gaaaccagat gaagtgatta cgatcgttgg acacggagca gaacaagtta
     1501 aagcacaact aggagaacgc agtaaatatg cgttacaagc agaacaattg ggaacaggac
     1561 atgctgtttt gcaagctgct tctttcttgg aaggaaaaaa aggaacaacg ttagttatca
     1621 gtggcgatac accattattg actactgaaa cgctgaacaa tctttttgaa tatcaccaag
     1681 gaaaaaatgc aagtgcaacg atcttgacgg cacaagcaga aaacccaaca ggatatggac
     1741 gaatcatccg tgaccatatt gggatcgtag aaaaaatcgt ggaacaaaaa gatgcaacac
     1801 cagaagaagc tcttgtacaa gaaatcaata caggaactta ctgttttgac aacgaagctt
     1861 tgttcgatgc gttaagcaaa gttggaacaa acaatgctca aggtgaatac tatctaacag
     1921 atatcatcga aatcttaaaa gaagaaggcc atacagttgc tgcttatcaa acggatgatt
     1981 ttgaagaatc aatgggagtc aatgatcgga tcgcgttagc taaagcaaat gagatcatga
     2041 gaaagcgaat taaccagatg catatggtca atggcgtaag ctttgtcgat tcagcaacga
     2101 catacatcga tgcaggcgta gaaatcggtc cagacacatt gatcgaagca ggtgtacaaa
     2161 tccaaggaaa tacagtcatt ggttctgatt gtgtcatcgg gtcacattca aaaatcgtag
     2221 acagtaggat tgaggatcac gtcgtcattg aaaactcagt cattgaaagc agccatgtca
     2281 agaaacatgc agatgtggga ccttatgcac atttacgacc aaaagctgaa attggcgaaa
     2341 acgtccatat cggaaacttt gtggaagtga aaaatgctca aatcggcaaa ggaactaaag
     2401 tcggtcactt gacttatgtt ggtgatgcta cgctgggaga agaaatcaat gtcggatgtg
     2461 gtgttgtctt cgtcaactat gatggaaaga acaaacatca tacgactgtc ggcgaccaca
     2521 gctttattgg ttccagcacg aatatcatcg gacctgtcga ggtagctaaa aacagttcga
     2581 ttgctgccgg gtcaacaatt acagacaata ttccagaata tgcgctggca attgcgagag
     2641 caagacaagt caataaagaa ggatatgcaa aaaaactccc ttatctaaac taagactttt
     2701 gcaaaatcac ttgatttatt taaaaaatat gactactatt tattaggtag aattctagag
     2761 ttgtgaaaaa gaaagtggag gtccccatgt caaaacatta ctttgaccca agattaaaaa
     2821 tttttgcgtt aaattccaat cgcccattag cagagaaaat tgcagcagca gtcggcgtcg
     2881 agttaggaaa atcatctgtt actcaattta gtgatggaga aattcaagta aacattgaag
     2941 aaagtattcg tggatcgcat gtctatgtta ttcaatcaac gagcagccca gtcaatgata
     3001 acttgatgga gttgctgatc atgattgacg cactgaaacg agcaagtgca aaaacaatca
     3061 acgtagtcat gccttattat ggctatgcgc gtcaagaccg taaagcacgt gcacgggaac
     3121 caatcacagc gaaattagta gcaaacatga ttcaaaaagc tggtgcaaca agaatgctga
     3181 ctttggacct tcatgcagtc caaatccaag gtttctttga tattccagtc gatcatttga
     3241 tgggtgcgcc acttattgcc aattatttct tagaaaaagg aatcaaaggc gatgatgttg
     3301 tcgttgtttc accagaccat ggtggtgtca cacgggcacg taaattagcg gaatttttga
     3361 aatctccgat tgcgatcata gacaaacgcc gtccaaaagc aaatgttgca gaagtaatga
     3421 atatcattgg acaagtcaaa ggcaaaactt gtgtactgat cgatgatatg atcgatacag
     3481 caggtacgat cacattggct gccaatgctt tgaaagaagc tggtgcaact agtgtgtatg
     3541 cttcatgtac acatccagta ttatccggcc ctgctttaca aagaatagaa gattcagcta
     3601 tcgagcgtct agttgttaca gactcgatct atcttccaga agatcgtaaa atcgacaaaa
     3661 tcgatgaagt aagtgttggc ggactaatgg gagatgcaat caaacgtatc catgaaaaca
     3721 aaccagttag tccattgttt gaaacaaaaa ataaataata aataaactca caaaggggag
     3781 tccaattggg ctgagattga atctatttct aaacccttcg tacctgtatc ggttatgcga
     3841 gcgtaggaat tgtgaataaa cagctgtttg actattttcg aaagctgctt cctcctttgt
     3901 gactttttta ttcaaaggag gaagtttttt tatgggaaaa aatagagttt gggtagaagg
     3961 aacgattgtt gcagcgttag cgatggtgtt gtcgttgatt ccgatccaga tcggcagcag
     4021 tttttctatt tcattagggc agattccatt gactcttttt gcgttaagaa gagggacaaa
     4081 ggcagggatg ctggcaggtt tcatttgggg catcctacat tttccgttgg gacaagttta
     4141 ttatttaagt gtcgttcaag tgttgattga gtacccgatt gcctatactt ttgcaggcgc
     4201 agcagggctc atggcttcaa atgtccaaaa aggctatgta gacgataata cgaaacaaat
     4261 tagaaaaagc atcgtgtttg gtgcattgat tggcgcactt tccagatatt tttggcattt
     4321 tattgcagga gtggttttct ggggagcgta tgcactatgg gggatgaatc cttggctctt
     4381 ttctttcgta atgaatggtg ccagcgggtt gagtacagct attgtaacga tcatcgtttt
     4441 attagggatc caacaaacga ctccttcgat ttttgtgcca gataaacgac aggagctgta
     4501 aataaaaaag aagcaagaaa cttgagacag aatcatgtcc tagtttcttg tttccttttt
     4561 attttttgat aagaaataga tctacttcaa tggcagataa gaaagatcgg catcttctac
     4621 caattgccag tgtcgatcgg tgacagagta gattaaagta gcgatgattt cctcttctcg
     4681 ataccgggtg ccttttaatt tccaaatata atcttcaatc acatagattg ggatacgata
     4741 tttaccatcg atcaaaccaa cttgatcaaa agaaaaaatg atcccttcct gcatcaaatt
     4801 acgcatttct ttcaataatt ccacactgat tttttcaaca ggtgtttctt ttttgaaaaa
     4861 agattttttt acattgatct tatctgacaa gatcgaactt aatttactag caaaaatcct
     4921 ataaaattca tctaaatagc gataataaat ccgattcagc tgatcattaa gatcgaagta
     4981 tacaaaatta ttctgtagtt tataaaaaaa cggcgaatga agatgtgttt tcatatgacc
     5041 aaaatataat aattctgaaa tttctaaagg tgtcaattct ttcagcatcg ttaaatcagt
     5101 aaagtcgatc cacttcaatt catcgaatcc acgcctgtta cgagaagagg aaaagaaacg
     5161 ctgaatattt tctgtaccac ggattacttt cagccctgta tgggattcat attctccagc
     5221 agaagcagat ggatcaagca agagcaagtt ttgaggaaaa tgcacgatac tgtttgcaaa
     5281 gtcagtagca gtcagccctt ttgttaaaac agcattactt gtggtatcaa tatgtacata
     5341 aactgactca gtcatcatgt tcctcctctt atttcctctt aatctctttt attttacaac
     5401 atttgcatta aaaaagcttg tttcttttat tttaaaattc cattacaaaa tcacgttttc
     5461 attgaaaaca ctctcatttt caattaattt ttgtgatttt tgtatttttt tatttaaagg
     5521 aggttttatg aattgttttc ttatggaatc tacaaagtcg aatacgagac tcatgacctt
     5581 tttatgacgt actggtatac ttgaagaaaa aggagagcgc gatggagata aaaaaagttt
     5641 acttttcaca gaaaatgtcg ctgtattcgc ctatccagca agcaaaacaa ccccttccat
     5701 tttttcaaag tcatcaagaa agtagagacg ctttttggcg ttctgcaaaa aagcaatgtg
     5761 cttttaaagc agtatcgaaa aaatataccg tctattttac tattgctaaa agtagaggag
     5821 atgttatttt taataatctg ttgattgaag gtgaaacttt tgaatggaaa aaattttttc
     5881 ggtatatgga agcttgcgct cgttttttta ttaaagaaaa ctgttgtttt ttatttcaat
     5941 ctcctctatc agaagaatgg ttgcgtattt ttcataaaaa cggctatcaa ggaaccacac
     6001 aattaaataa aaagctggtc tatcatacag cacttgtttt aggtggagga ggagcgcacg
     6061 gtgcttatca gattggtgtg tggcaggcgc tgaaggaaca tgatatcaac tttgagatta
     6121 tcacaggtac ctcggtaggt gcgttgaacg gcgcattgat tttacaagga gatatgaaaa
     6181 aagctgtgaa tttatggaaa aagctgtcga caagacaagt gcttgcgtta ccagaaatgg
     6241 ctgcagttga agaccttcgt gaacgcttct atcgagaaag acgccaaatg acgaaaacag
     6301 cactaattga aggcggggtt tcttctgctc cattggaaaa gatggtcaaa gacaatttgg
     6361 atttgtcctt actcaaacat aatcccaaaa tacgtttata tacggtgtct actaaactgc
     6421 cagaactagc ggaagtagtg actgatatcc aacagacaaa aacagaagaa ataccagatt
     6481 ggatactcgc ctctgcttca ttttatcccg ctatggctta tcgaaagatc ggaaaaaata
     6541 aatatgcaga cggtgggtat cgcaacaaaa tacctattga tattgcgatt aacaaaggag
     6601 caacagaggc atttgtcgta gatgtgcaag ggccaggccc agcgaaaaaa attcgtatgc
     6661 cagacatttt tatccattgg aaatgtcaaa cattgtggac actaggcagc tttctgcttt
     6721 ttgatagcca aaggaaccaa ttaaatttac aattaggcta tctggaaatg aaaaaacgat
     6781 taggctatta ctatgggaac tggtatacat ttcattctgt taaacctgca agaacatact
     6841 ggcgaggatt tttgtcttac ttaacaaaag aaattcagtt agaattatct tttttcaaaa
     6901 gcataaaatt ttggcaaaaa ctgcgtaatt tatataagaa cagggtcgta ccagagacat
     6961 gcggattggc gatgctagaa cttttagcaa aaaaacattt tttactgccc aatgaaatct
     7021 atcaagtaga catgatgatt caaatgatct gccaacaaga tataaaaagt atgccggatg
     7081 atttattggc acaaattggt caattaagta ctgaagaatg gcgaaggtat cgaaaatacc
     7141 agcaaaaaat acaaaatgag cgcacgaaaa ctgtttattt tgattatctg ctacaaggaa
     7201 aacgcaagga tcgtttgcag catgaattgc ttaagcaacc agttgacaca ttactgatcc
     7261 tatatttata ttatctcaag gaggaacaac catggcacaa gaattttcat atgaaatcgt
     7321 agaagaaatc gctatattgt cagaaaacaa caaggggtgg cgtaaagaac tgaacctagt
     7381 cagctggaat ggtcgcccgc caaaattcga tcttcgagat tgggcgccag accatgaaaa
     7441 aatggggaaa ggcctgacat tgacgaacga agagtttgaa caactgcaaa aagcaataga
     7501 aaatatgtaa tgaggaagga ccctgtcgaa tgaaaagtat gactggtttt ggcaaagcca
     7561 tccgcgaaac aaaagcgtat cagttagata ttgaaataaa aagtgtcaac cagcgttttc
     7621 tagatgttca aatacgaagt cctaaaatcc tgaactttgt tgaaaacgat tttcgtcagc
     7681 ttattaaaca gcagttaagc cgcggtcgaa tcgaagtatt tgtgaatcta gcttatattg
     7741 gcaaaaacga aaaacagatc gtcatcaact gggaattgat tgattcactt atcactcaat
     7801 tgacagacgg aattcaacaa cgatacggta gtcaaaatca agtgtcaatt agtcggatgg
     7861 cggaacgctt cgtgctggac gaaaacttcg taacggtaga agaaaaaccg gaagagagca
     7921 tggaagaatt ggcacagtta gctctagata cattgaaaag cgcacttgat tctattgagg
     7981 cgagtcgaga aaaagaaggt caagctttag ctttagtgtt gaaaaagaat actgaagaat
     8041 tcaaagaagt actaaaaaaa ctcgatagtt ttgtagaaat ctatgaaaag gaatttcaaa
     8101 taaggtatca aaaaaagctg gaggaatttc ttggagcgaa agtcgatgag caacggttgt
     8161 tgacggagct agccatcttg ttggaacgcg gagatattca cgaggaactg gatcgtctgg
     8221 cgatccatgt acaaaaacta gatgaactta tcgaagcaca atgtcctgta ggtcgggaat
     8281 tagatttcct gatccaagaa atgaatcggg aaatcaacac gatcggttcc aaatcaaatg
     8341 caatcgaaat taaaaatcaa gtcgtccagt ctaagacgat cttagaaaaa atcagagaac
     8401 aagtacaaaa tattgaatga cgaaagaggt agagataatg aaatattatg acgtaacgtt
     8461 ccacgaatta agtggaaaaa ctgtggttaa acgggatatc ccttctgaaa aaaacggctt
     8521 tgacgtatgg aaagatgcct gtgcagacta taatgagaac gaactgttta ttttgatcaa
     8581 cgacggtgct tacgtgacga tgaatcggaa atttattgtt cgaattgaca cagaagaggt
     8641 agaagatccg acagaaaaag cacgttctcg aaaagatgag atcatgggtg tagtgaatac
     8701 tttgtccaat atgggctttt agcaaggatg ctgaacaaag aaatacaata aataaacaga
     8761 attggatttc ttcgcttagc ttctttgctc taggaataca tttaaaggtt gtgacaaaat
     8821 tcgtgtcaca atcttttttt attttgaata agtgatgttc aaaaaccatt ttctcggtat
     8881 taagccaaat ttatgcaaaa atgtgagaaa ctattttcac aaaatttttc ttcatactcg
     8941 aaactaatcg gcttcttcac gacctcttat acacggcgtt caaaagcttg catctgcgat
     9001 aataacccca accaaacgaa aaacatgaga agctgtttat gtttggctgt tcttattact
     9061 tgatgcagga cagctttttt acgacctctt ggtacagggt agctttttta ccaatttcta
     9121 gctataatta aagataacct tgaaagttgc agaaaaaaag tgcaaaataa acgataagta
     9181 caaaaagact agttgaaagg acgtcgtcat gtcagagcgt ggattattaa tcgtgttgtc
     9241 tggtccatcc ggtgtaggaa aaggcacagt acgaaaagca atttttgaga gtgaggataa
     9301 tgattttcag tattcgatct caatgactac ccgtaaaatg cgtgaagggg aggtcgaagg
     9361 cgttgattat ttcttccgaa gcaaagaaga gtttgaatca atgatcgaag ctggagagat
     9421 gctggaatac gcagaatatg tcgggaatta ttatggaact cctttgtcat atgtcaacaa
     9481 gacattggat gaaggaaaag atgttttctt ggagattgaa gtccaaggag caaaacaagt
     9541 caaagaaaaa gtgcccgatg gtgtattcat ctttttgact ccgccagatt tagcggaatt
     9601 aaaatcacgg atcgtaggcc gaggaacaga tgcagatgaa gtaatcgaaa aacgaatgaa
     9661 agttgcaaaa gaagaaatcg aaatgatggc tctttacgac tatgctgttg taaatgatga
     9721 agtgccgcta gcagttgaac gaatcaaaaa cattattgcc agtgaacatt ttcgagtaga
     9781 tcgcgtaatc ggtaaatata ttaaaatgtt aaaggagatg tagtcttatg atgttaaaac
     9841 cttcaattga ctcactatta gatcgtgtaa attcaaaata ttctctagtg attttagcaa
     9901 gtaaacgcgc gcatgaattg gatgcaagtg cacagcctac tctagattct tttgattcag
     9961 taaaaagcgt cggtcaagct ttagaagaaa tcgatgcagg aaatgtagtc aatgatccac
    10021 atcctgaatt aaaacgtgaa cgtttgaaga tggaagaaga ggaacgtcaa gcacaaaaag
    10081 agcgtgaaca acacgaattg gaatcacgta tccgtgaaga gcaaaaaatg taataaagaa
    10141 aaatagagcg aacatctaaa aagaaaagca aacccagctg tttccttttc aaacattcgc
    10201 cacttattgt ctggtagaaa aggctgtgtc tgaacttttt gacacagttc ttttttttct
    10261 tatctgctgt taattttttg ttaggatttg gcagaagata agacaaatcc atcgatctct
    10321 agtaaaatag gcaaggaact ttttgtcgct tgataagaaa agacaaaaaa aggaggaaac
    10381 gaaccatgac attaacagct gaagtcattg tggacgtacc tactatgcag accgatcagc
    10441 cattcactta tttggttccc tctgaaatgg aaacagcttt gcaagtcggg atgcgagtag
    10501 aagtcccttt tggtaatggc aatcgtcatg tccaaggatt tgttatggct atccaaaaat
    10561 cagaagaatc agctaatcca tctttgaagg cagttattcg tttgctagac cttgctccgg
    10621 tggtcaatga agaattactt tcattagctg attatatgaa aaaaattact tacgcattca
    10681 aaatcacttg tctacaaacg atgcttccga gtgtgatgaa agcagaatat gataaattga
    10741 tctatccttt ggcagacaca ccagaagttc aggtattgtt tgatcagcga gaagtgattt
    10801 cctggaagga agcagaagaa gaaggcagcc tttctcaatt gattcgctgg cgccaagagc
    10861 aattagtaga tatcaaatat gaagtccata cacgaaataa agtaaaaacg attcgtttgg
    10921 tccgttcttt gttaactgaa aagcaaatcg aagaagaatg ggcaaaatta cggcaaaatg
    10981 ccaagaaaca aaaagaactt cttttgtgtt tatctgaact gagccaagaa gaaccaattg
    11041 cttatttcaa aaataaagga atcagtactg ctgtcttgaa tcaaggaaaa gaaaagggct
    11101 ggctggagtt tatagaatca gaacgttatc gcgaccctta taaagaccga gtatttgacc
    11161 aaacgacggc attggaactg aatgcagaac aaaaaaatgc tgtcgaacaa atcatcacag
    11221 caggtcagca gcaaaaagat caggtctttt tgctggaggg aatcactggc agcgggaaaa
    11281 cagaagtata tcttcaggca atcgctgatg ttttgtcaga aaataaaacg gctattatgt
    11341 tagtgcctga aatcgcattg actccgcaaa tggtcgagcg attcaaagga cgcttcggag
    11401 aatcagttgc ggtcttacat agcggacttt cccaaggtga aaaatatgat gaatggcgca
    11461 agattgagcg ggaagaagcg caagtagtag taggcgcacg atctgctatt tttgccccgc
    11521 tgaaaaatat cgggctgatc attatcgatg aagaacatga atcaagttac aaacaagatg
    11581 agacaccgcg ttatcatgcc agagatctag caatttggcg cagtaaatat catcattgcc
    11641 caatagttct aggaagcgcg acgccctctt tagaatcaag agcacgagct caaaaaggcg
    11701 tttatcagtt attgcagtta aaccaccgag ccaaagcagc tgcacagctt ccagctattg
    11761 agatcgtaga tatgcgtgaa gagtttcaga atcatcggac ctctactttt tctgctaatt
    11821 tgcaggaaaa aatacaaaat cgattagaca aaaaagaaca aactgtccta ctgctcaatc
    11881 gtcgaggata ttcgtctttt gtgatgtgta gggactgtgg atttgttctt ccatgtccta
    11941 attgcgatat ctctcttaca ctgcatatgg atacacgttc aatgcgctgc cattattgcg
    12001 gacatgaaga acctattccc aatcgctgtc ctaattgtgg cggcaataaa atccgctact
    12061 atggaacagg tactcaaaaa gtagaagagg aactccgaga actttatccg caagctagga
    12121 ttttgcgtat ggatgtagat accactcgaa gaaaaggggc acacgaacag attttacaaa
    12181 aattcggtgc aaaagaagca gatatattat tagggacaca aatgattgct aaaggtttgg
    12241 actttccaga agtgacatta gttggggtgt tgaatgctga tacctccctg aatttaccgg
    12301 attttcgttc tagtgagcat acattccaat tgcttaccca agtttcagga cgagcagggc
    12361 gagcggaaaa agcaggcgaa gtcgtgatcc aaacattcaa tcctcaacat tacgccatag
    12421 aattggctaa aaaacaaaat tatgagcagt tttatcagca ggaaatgcat gtacggcatc
    12481 gtggaggcta tcctccttat tactttactg tgaaaataac cgccagccat cctgaagaac
    12541 aagtagcggc aaagaaaatc tttcagattg ccaatcagct aaaagaagtg ttaagccctc
    12601 aaagcttgct attagggcca acgcccagca tgattttgag agtgaaaaac cgatattatt
    12661 atcaactaat catcaaatat aaacatgaac cgaatcttcc tcatgttttg gatgaaattt
    12721 tgaatggaag ccagaaagaa cagcgacaag gtctttttgt ggcaattgac aacgaaccat
    12781 taaactttat ttaagaaagg gccaaattat gcgctatcct atattgatcc accctaatga
    12841 taaattaaaa agaactgctc agccaatcga tgtaatcacg gacgagacaa tcgctttatt
    12901 agacaattta tatgagacaa tgattgcgaa tgacggaata gggatcgctg ctcctcaagt
    12961 aggacaaaac aagagaattg ctgtgatcga agtagatgaa ggcgagaaat ttgaactgat
    13021 taacccagaa atcatcgaag caaaaggtga aagccttgac gtagaaggtt gtctcagtat
    13081 tccgcatgtc tatggaacgg tcaagcgagc agatgaagtg accgtgcgtt attatgatcg
    13141 agacggagaa gagatagaag tgacggcttt tggctattta gcccgtgcct tccagcatga
    13201 aatcgatcac ttagacggta tcttgttcat tgagaaaatg atacagcaga tcccggaaga
    13261 agaacttgaa gaatatatgg aggaacatct tgatgactaa gctagtattt atgggcacac
    13321 ctgctttttc tgtgccgatt cttgaagggc tgatagaatc aggttacgag atccaggcag
    13381 tggtaacaca gcccgaccgt ccagttgggc ggaaacgagt aattacgccg acaccggtaa
    13441 aaaaagcggc tctgaagcac ggtatccgtg tgctgcagcc agagaaaatc tccggttcgc
    13501 cagaaatgga agaaatcatt gaattggtgc ctgatgtgat cgtcactgcg gcatttggcc
    13561 aattcttgcc tgaaaagtta ttgcaagtac caaaattagg ggcaatcaat gtccatgctt
    13621 ctttacttcc taaatatcgg ggaggtgcgc ctgttcatta tgccatcatg aatggggaga
    13681 aagaaactgg tgtaacgatc atggaaatga tcaaaaaaat ggatgcaggc ggaatctatg
    13741 cgcaagaaag cataccaatc actaaacaag atgatgtagg gacgatgttt gaaaagctta
    13801 gccttttagg aagaaagcta ttgctagaaa cattgcccaa tattttggat ggacaaaaac
    13861 cagtacctca agacgagtca gaagcaactt tttcgccaaa catcacacgg gaacaagaag
    13921 caatcgattg gcataaaact gcagaagaaa ttgacaacca agtacgcgga atgcgtcctt
    13981 ggccaattgc attcacgacc tatgagggca tacgctggaa attattatcg gttgaagtat
    14041 tggcagaaac tacagacaaa gcacctggta cgatcatccg gaaagacaaa aaaaatctat
    14101 ggattgcttg cggtaaacag accgtcttag ccatcaatga attacagcca gctggtaaag
    14161 gaaaacaggc cgtacatgat ttccttaacg gaagcggtca gcaagttcag attggtcagc
    14221 aggtgaaata atgaaaaaaa tccctaaaaa aatgaaaaaa tcagtacgat atatggcatt
    14281 actgacatta gagcgagtga acaagggtgg cgcctactcg aatcttttgt tgaatgagat
    14341 gatcaaacaa agcgagctaa atcaaaaaga tgtgggttta tttacagaat tgatctatgg
    14401 tacgattagc catcagcgac tattggaatt ttacgttgca ccattgatcg ctaaagcaaa
    14461 aaaagtggat gactgggtca agatgcttct ttatctttct gtataccaat tagaattttt
    14521 agataaagtc cctgcacatg ctattttaaa cgaggcggtc gaaatcgcaa aagtaaaagg
    14581 aaatcctggg acagggaaat ttgtaaatgg tgtcttgagg aattatcagc gtcaaggagc
    14641 accagatctg caaggcattg ctgatcctat cgaaagactt tctgtcaaaa tcagcctgcc
    14701 tctatggatg acgaaacgac tggtggatca aatcggatac gaagaaactg aaaagctcgg
    14761 tttatcgcta tatcagccta gccatgctag tgcccgagta gatacacgcc gactttccag
    14821 agatcaggcc atcactgttt tgcaagaaga agagatcaca gctgaaaaaa gccaagtcag
    14881 tccatacggt atagtagcga aaaaagggca tttagcttct agctcattgt tccacgatgg
    14941 cgtcatgacg atccaagatg aaagttcgat gcttgtagcg ccgtctatgc aaatcgagcc
    15001 tgaacatcat gtcttagatg cctgtgcagc acccggaggt aagacgacgc atattgcttc
    15061 ttttttagat ccgtcatgcg gtggagaagt ggtcgcttta gatgtacacg aacacaaagt
    15121 caaactcatt gaagataatg cagcacgtct aggtgtctct tctgctgtct acgctcaaaa
    15181 gatggatgct agagaagtca gtaaacagtt cgaagcagag cagtttgatc ggattttagt
    15241 agacgcgcct tgttcaggtc ttgggctgct tcggcgaaaa ccagatattc gttataaaaa
    15301 acagccggat gaattagcga atttaccaaa aatccaattg gaaattttag aaagctgcgc
    15361 accaacttta aagtcttccg gtattctgac ttatagcact tgtacgatct taaaagaaga
    15421 aaaccaagac gttgtttctg ctttcttgga aagacaccct gattttgaga ggattgatgt
    15481 tttgaccaat cctatgctac aatcatcaat cgaagagaaa atgttgactt tgtatccgca
    15541 tcagttttac acagatggat ttttcatttg ttgtttgcgc aaaaagtaat gaggtgaaaa
    15601 aatgcagatt gaatatcaat cagatgtagg aagacgacgt aacacaaatc aagactatgc
    15661 cagtgtattt accagccgtt caggtctcaa gcttgctgta ttagcagacg ggatgggtgg
    15721 tcatcgtgcc ggagacattg ccagtcagat ggcggtaacg aatctaggcg aagcttggga
    15781 aaaacaagaa gctgttgacg atgaaaaaat cgcccaatgg ttcattcaat cgattcaaga
    15841 agaaaatatg ctgatttatc agcgtggaca agaacagccg gaatataacg gtatgggaac
    15901 aacaatcgta gcggccgctc tgtcagatga ctggttcacg attgccaatg ttggggatag
    15961 cagagcttat ttgattcgtg aagataaaat catgcaattg acggaggatc attctttagt
    16021 caatgaacta gtcaaatcag gagaaatcag cgaagaaatg gcagtgaacc atccaaggaa
    16081 aaatatttta acacgttctg tcggcatgcc tggaacagtc gaagtagatg tttctactca
    16141 tctttggcaa ctaaaagatc gactgctgtt atgttcagat ggcttgacga acatgctgtc
    16201 tgaagaagaa ataggaacga tcatcaatca agaaggccca ttgtctcata aagtaactga
    16261 gctgattgat cgtgccaatg aagcaggcgg agcggataat atcaccgttc ttcttatcga
    16321 atataaggag gacagtgtat gatcgagttg ggcaaaaaat taaatggccg ttatcaaatc
    16381 actggaaata tcggtagcgg tggtatggca aatgtatttt tggcgcacga tttaatcttg
    16441 gatcgtgatg tagcagttaa agtcttacga tttgactttc aaaatgacca gactgcgatt
    16501 cgacgatttc agagagaagc tttagcagca acagaactcg ttcatccaaa cattgttagt
    16561 gtttacgatg ttggtgaaga agacaacatg cagtatctgg tgatggaata cgtcaaagga
    16621 atggacttga aacgatatat ccaaacacat tatcctgttc cttatgaaac agcagtaaat
    16681 attatgcagc agatcctttc tgctatttct ttagctcata accatcaaat catccaccga
    16741 gatttgaaac cccaaaacgt actgatcgat aatgaaggtg ttgtaaaaat cacggatttc
    16801 gggattgcta tcgctttatc tgaaacatcg atcacgcaga cgaatacgat gctaggatct
    16861 gttcattatc tgtcccctga gcaggccaga ggaagtatgg caacgaagca gtcagatatt
    16921 tatgctttag ggatcatcct ttatgagatg ttgacgggta gcgtaccgtt tgatggtgaa
    16981 tctgctgtaa caattgcttt aaaacatttt caagatgatc ttccatctat aaaagcactt
    17041 gatccaaatg tgccacaagc actggaaaac gtgattttac gggcaacggc taaagaacct
    17101 gcagaccgtt ataagtcagc cgaggaaatg tctgatgatc tttctacagt attatcacca
    17161 gctcgtgcaa acgaagaaaa atggcagcct catgtgatgg ataatgaaac aaaagttatc
    17221 acacccctta cggaagatgc accaatgcct gattcattca aatctatgcc tctgccaaaa
    17281 gacaagccag aaaacgaaac cttaccagag gaggaagaag ttctgcctga tcctaaaaag
    17341 aaaggacaga aaaaatggtg gctgatccta ttagctattc ttgctgtttt aggaatcgga
    17401 acagctgtat tctttgcttc tggcgggcga ggagaagttg ttgttcctga tgtttctgat
    17461 ctttctgaag cttctgctag agaaacactc gcaaaagcga aattagaagt agcaacaaaa
    17521 acagaagaaa ttgcagatga caaaatcgaa gaaggtaatg tagtgaagac agacccggca
    17581 gcgggaacga cagtcaagca aaaacgcgaa gtcactctgt atattagtag cggcacgaaa
    17641 aaaatcaaat tagatgatta cagtggtaaa agttatgaag atgccgaagc aagtttgaaa
    17701 gaattagggt tttctgctga cttgattcaa aaagaagagg aattcagttc agaggtagaa
    17761 agcggaatga tcatttcgca atctccatca gaagatacag aagtcgatcc taaaacagac
    17821 acgattactt tcacagtcag caaaggacca gaaacagtga ctttgagtag tcatgtagga
    17881 gaaaactacg aaactgttcg taattggctt gttacacaag gattcaatat caataatatt
    17941 agtgctactt atgactatag cgacactgta ccatcaggtt cgatcatcag tcaggatcca
    18001 gcctcgggtg aagttgtagc agaagaaact tacatcaatt ttgtcgtaag ccaaggaaca
    18061 gagcctaaat tgaatgatat ctccggctat acaaaaagcg aagcacaaag ttatttagct
    18121 agtgtcggtg cagaatatat cgggcatgag acttccgaat attcaaacac ggtggataaa
    18181 gataaagtga ttcgtacagt accaggggca ggaacgacaa ttaccaaagg aatggttgtg
    18241 aacgtaattt actctaaagg accagatcca gcgtcttcta gttctgaaaa agactcttca
    18301 gacagttcta gcagctcaag tagctccagt agcacaagca actcttcaaa agaagaatct
    18361 tccagctcca caagttcatc agctagcacc tcagaaagtt caaaataagt gctgaataaa
    18421 atatatcaga agggaatgcg acattgattt gttgcattct cttttttgtg tttctatctc
    18481 ttttgatcaa ttttcaagcc atatactttg agaagtttgc atggaataac acctactttc
    18541 ctcttcgtat ggtaaaataa acaaaaacgg aggtggcact tatcaaagga caaatcagaa
    18601 aagcaatcag cggtttttac tatgtatacg caaacggaga gacctatcaa acaagaggaa
    18661 gaggaaattt ccggaatcgg aaaatcacgc cgcttgtcgg agatcaggtg atctttgaga
    18721 gtgaaaatca aacagacggc tatcttctgg aaatcttgcc gagaaaaaat caattagttc
    18781 gcccaccagt cacaaatgtt gatcttggaa tcattgtcac aagtatcgta gagcctgcat
    18841 tttcttataa tctacttgac cgctttctag ttaccttgga atacgagtcg atcgaaccga
    18901 ttattttttt gacaaagacc gacttgtcag acgacccaga aaaaatcgaa gagatcaagc
    18961 aaacctatac taaaataggc tatccggtga ttgtacccaa tcatacgggt gatacgacag
    19021 aactgatccg ttacttccca gaacggctat cggtctttat gggtcagtct ggagcaggaa
    19081 aatcgacgct actgaatcaa atatcaccgg aactgaattt agcaacagga gagatttctg
    19141 atgcacttgg aagaggacgg catacaacac gtcatgtgga actgattcct atctatggcg
    19201 ggttagtagc agatacacca ggatttagct cgattgactt tttgacaatt gaagcaggag
    19261 aactgccgag acaatttcct gaatttgtag aagcttctgt ccattgcaaa tttagagagt
    19321 gcatgcatgc taaagaacct ggctgcgaag taaaacatag agtagaaaca ggtggtatcg
    19381 cacagacacg ttacgacaat tatttgcaat tcttacaaga aatcgaaaaa agaaaaccaa
    19441 tgtatgcaaa aaaagaaggt aaacgaaaat aggaggaaac aagaatgaag atagcaccat
    19501 ccattttgag tgcagatttt gccaatttac aaagagacat tgagttagtg gaaaaaggag
    19561 gcgcagatta catccacgta gatgtaatgg atggccaatt tgtcccaaat atcacattag
    19621 ggccaaatat tgttcaggca atccgtcctg ttactaaatt accgttagac gtccatctga
    19681 tgatcgttga tccagaaaac tacattccag catttgcaaa agctggagca gatatcatta
    19741 ctgtccacgt cgaagcaact cctcatattc atcgggcact tcaaatgaca aaaagcttag
    19801 gagttaaagc tggagtagtt atcaatccag gaacaccagt ctccatgatc aaacatgttt
    19861 tgcctttggc tgatcaagta ctagtcatga ctgtcaatcc tgggttcggc ggacaaagct
    19921 ttattgaaga aacagtagaa aaaattacgg aactcgctga tctacgtaga gagaacaatt
    19981 ggacttatgc aatcgaagta gatggcggaa tcgttccaga aacagcaaaa atctgtaaag
    20041 atgcaggggc ggatgtcttc gttgcaggat catatatcta tggagccgaa aatccagtcg
    20101 gacaaatcga acgattaaaa gaggcattag actaaatgaa aattttatta gctgctggag
    20161 gtaatccgca aaactggccg cagtctctgc tgtccaaata cgatgcctat gtcgggatag
    20221 accgtggtgc gctgtacctg cttcaagcag gctacccagt agatctggca gtgggcgatt
    20281 ttgattcatt gtcgaacgaa gagcagaagg aagtatttgc aaaagccaaa gagtataccc
    20341 gttcccctgc agaaaaagac gatacagata cacagttagg tatcttaaaa acatttgaac
    20401 gttacccgca agcggaagtc actttgattg gagctacggg cggaagattg gatcatcttt
    20461 tagccaatct ctggttaggt atggaacctc gcttcaaacc ctttgcccat cagcttatta
    20521 tcagagattg tcaaaattat ttgtcttatt atttgcctgg agagcatata gtaagacaga
    20581 taccagatat gaagtattta gcttattgct gcttgactcc cgtctcgaat cttacattaa
    20641 aaggcagcaa gtatgtattg gaaaataaag aagtagaata tccgacatct tatgcaagta
    20701 acgaatttct ttcagatacc gcagaatttt ctttttcaga tggtatgatt gcagttattc
    20761 aaagcaaaga ttgatccata aacaaataaa aaaatttgtc tttcaggcta aaaatgacag
    20821 aatctattat ttgctaaaaa aaagcttaag aggagcaact taccttttgt ttcacggcat
    20881 ttagtgataa aattgaacac aagagtctaa ttaattcgag gtgtctttgt gaagaagttc
    20941 aatcctaata aaaatattat tattaccttg gttattgtta tcatcgtggt gacgatacta
    21001 agtgtgaccg ctgcgcagcg agcgaatgaa ggaaagccca atttcttcca atccatcgtt
    21061 aatgacagtg ttgcaatggt tgataaaaca atctctgccc cagtacggtg ggtacgaaat
    21121 ggagtagatt ctgtccataa tcttttttca acttatgccg aaaatgagcg gctaaaagaa
    21181 aaaatcgata gctatgatga cgtttcacag caaaacaaga attacgagag agaaatcgaa
    21241 gcgttgaaaa aagaacttga gttgaatcag acgctgacta gttacgaaaa agtaacggca
    21301 aatgtcatta ctcgttcgcc tgacagttgg caggacatgc tgatcgtaga caagggttct
    21361 aaagacggta tcgaagcaaa tatggcagtc atgtcgcaaa aaggattgat tggccgagtg
    21421 atcgaggtca atacggcttc gtctaaaatc gaattactgt catcctctaa tgaaagctcc
    21481 aatcattttc cagtacgggt atcttcggct aatggcgaag cgtttggttt gcttaaaaac
    21541 tatgatgaaa agctccatgc cttagtggtg acccaattaa ctggtgatac ggatatcaaa
    21601 gaaggggatg ttgtccagac atccggtctt ggagggaatt ctccagctaa cttgccgatc
    21661 ggtacggtta ttaaaacgaa accagatagt tatgggctgg atcgggaagt ttatgtgaaa
    21721 ccttatgcag aaatgtatga cgtgtcagtt gtgacgattg tccaacggct agcggaggat
    21781 tagtccatgt tgaagaaaga aacaatgaag tactatctgc caatcgtttt gttctttttg
    21841 atgttgatag atggtcattt aacaagaatg ctaggggagt ggtcgaaagg cacctatatg
    21901 tcaaatgccc actttctgat attggcatta ttatgttgca gtatggcgtt tgaaaaacgt
    21961 tatttactga ttaccacgat tgttctcggg gctatctatg acgcttacta tattggcgtt
    22021 atcggtatct atgcagtagc tctcccttta attgtatggt tgatgtatgt aatgaaagac
    22081 gttatccatg tcaacatctt tactgaattt ttcagtatga tcatctttgt cacgggttat
    22141 gaattgttta cgatggtggt ccagttgatt tttaaattag cagtagtaaa taacacgtat
    22201 tttattacaa ggtttt
//
LOCUS       DAHHZJ010000033        21927 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.034, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000033 DAHHZJ010000000
VERSION     DAHHZJ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.034"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            211..1215
                     /locus_tag="NBL02_001006"
     CDS             211..1215
                     /locus_tag="NBL02_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2571711.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            1216..2160
                     /locus_tag="NBL02_001007"
     CDS             1216..2160
                     /locus_tag="NBL02_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2571712.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            2160..3122
                     /locus_tag="NBL02_001008"
     CDS             2160..3122
                     /locus_tag="NBL02_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2571713.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            3149..4063
                     /locus_tag="NBL02_001009"
     CDS             3149..4063
                     /locus_tag="NBL02_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2571714.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            4089..5870
                     /locus_tag="NBL02_001010"
     CDS             4089..5870
                     /locus_tag="NBL02_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCD2571715.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            6218..6904
                     /locus_tag="NBL02_001011"
     CDS             6218..6904
                     /locus_tag="NBL02_001011"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCD2571716.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            6924..10505
                     /gene="smc"
                     /locus_tag="NBL02_001012"
     CDS             6924..10505
                     /gene="smc"
                     /locus_tag="NBL02_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HCD2571717.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            10514..11323
                     /locus_tag="NBL02_001013"
     CDS             10514..11323
                     /locus_tag="NBL02_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCD2571718.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            11334..12332
                     /gene="ftsY"
                     /locus_tag="NBL02_001014"
     CDS             11334..12332
                     /gene="ftsY"
                     /locus_tag="NBL02_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCD2571719.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            12519..14786
                     /gene="gshAB"
                     /locus_tag="NBL02_001015"
     CDS             12519..14786
                     /gene="gshAB"
                     /locus_tag="NBL02_001015"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HCD2571720.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(14875..15327)
                     /locus_tag="NBL02_001016"
     CDS             complement(14875..15327)
                     /locus_tag="NBL02_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HCD2571721.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            15477..16427
                     /locus_tag="NBL02_001017"
     CDS             15477..16427
                     /locus_tag="NBL02_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCD2571722.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            16420..17379
                     /locus_tag="NBL02_001018"
     CDS             16420..17379
                     /locus_tag="NBL02_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCD2571723.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            17376..18131
                     /locus_tag="NBL02_001019"
     CDS             17376..18131
                     /locus_tag="NBL02_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD2571724.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHIGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            18154..19107
                     /locus_tag="NBL02_001020"
     CDS             18154..19107
                     /locus_tag="NBL02_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HCD2571725.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(19751..20047)
                     /locus_tag="NBL02_001021"
     CDS             complement(19751..20047)
                     /locus_tag="NBL02_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HCD2571726.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            20402..20758
                     /locus_tag="NBL02_001022"
     CDS             20402..20758
                     /locus_tag="NBL02_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCD2571727.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            20768..21667
                     /locus_tag="NBL02_001023"
     CDS             20768..21667
                     /locus_tag="NBL02_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HCD2571728.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT         7247 a         3867 c         4665 g         6148 t
ORIGIN      
        1 tttaatcttt ttgacacgta aaagtcattt ttctgataat tctggcgata aacagaaatt
       61 atgttgcaaa gtaaagcggt tttctatata attattaata attcattaca caaagataag
      121 aataaccttt gttataaaac gatttattct gtgtaatgtc cttttttatt aagaaaaact
      181 tccatttagg aagcagagag gggtattcga ttgtccgata accaattatt agaagttcag
      241 aacctacata caggattccg actcaaagat gactattacg atgcagtaga tgacgtttca
      301 tttactttag acaaaaacga gattttagca atcgttgggg aatctgggtg cgggaaaagt
      361 accttggcta ctacgattat gggattactt gatcctaata acacaaaaat tacaggtgag
      421 attttatata acgatctgaa tttaatcgac ctgaacgaaa cgctctataa taaaatccgc
      481 ggaaatgata tcggaatgat tttccaagat ccgttgtctg cattaaatcc tctcatgaga
      541 atcgaagacc agatcaaaga aggactttcc tatcacacaa agatgaatgc agaacagcgt
      601 caagctcgtg cactagaatt gctagaacaa gtagggattc ctaatcctgc gagagttgga
      661 cgccaatatc cccatgaact atcaggcggt atgagacaac gtgtcatcat cgcaatcgca
      721 atcgcctgca aacctccaat cgtcattgca gatgaaccga cgacagccct agatgtaacc
      781 atccaagcac agattcttga tttactgaaa gaccttcagg aagaaacaaa aacagggatc
      841 atcttgatca ctcatgactt aggagtcgta gcagaaatgg ctgatcgagt ggcagtgatg
      901 tatggcggac agttcgttga agtagcaggt gtaaaagaac tgtttgatca tcctaagcat
      961 ccttatacac gctctttact gaactctata ccacaagaag gaaatcatga tgcagaactc
     1021 catgtgatcg aaggtgtcgt tccttctctg aaaaacatgc ctcgtaaagg ttgtcggttt
     1081 gcaccacgta ttccatggat accagcaagc gatcatgaag aagagcctat ccttcatgaa
     1141 gtcgcaccga accacttggt acgttgctcc tgctataagc atttccactt tagagacgaa
     1201 aaaggagagg tgtagatgac agaattaatc acaatcaaag acctgaaagt tcattaccct
     1261 attcgcagtg gattcttcaa tcgagtaacg gatcatgtat atgctgtaga tggtgtggat
     1321 tttattatcg aaaaaggaaa gacatatggt ttagtgggag agtctggctc tggaaaatct
     1381 actacgggaa aagccgttgt gggattagaa aaggtaacag ccggacaaat catgtatgaa
     1441 ggaaaagatg taacgaagcg aagcaaccgc aaaaaaatgg gttataacaa ggatgtgcag
     1501 atgatcttcc aagattcaat gtccagtttg aatcctaaaa agcgagtgtt ggatatcatt
     1561 gctgaaccca tccgaaattt tgaacggtta agcgatcaag aagaaaagaa aaaggtaaaa
     1621 ggactgctag atatcgttgg gatgccggaa gatgcgctat ataaatatcc tcatgaattt
     1681 tcaggcggac aacgtcaacg tctaggagta gcccgtgctg ttgctaccaa tccaaaactg
     1741 atcgtagccg atgaacctgt atctgctctg gatctatctg ttcaagcgca agtattgaat
     1801 ttcatgaaac gaatccagca agaatacggc ctcagctatt tattcatttc acatgattta
     1861 ggtgtagtaa aacacatgtg cgacaatatc gcaattatgt acaaaggtcg atttgtagaa
     1921 atcggtacaa gagaagacat ttataatgac ccgcgacata tctatacgaa acgtctgctt
     1981 tcagctattc cgcaaatcga tgtagaaaat agggaaagcc acaaagaaaa tcgacgaaga
     2041 gtagaacaag agtatatcca aaaccaaaaa gaatactatg atgcgactgg tcgagtctac
     2101 gatttgcgta cgatcacaac tacacacaaa gtggcgttga aagatggagg tgcaagctaa
     2161 tgtggaaaac gattctgcga cgtgttttat tgatgatccc gcaagttatt attttaagtg
     2221 ttctgatctt cttattagcc caagcgatgc cgggggatcc atttaccggt ttgatcaatc
     2281 caaatcagga tccggcagtg atcgaacaga tgcgacaagc agctggcttg aatgacccat
     2341 ggtatgtcca gtacttccgt tggatcggaa atgctttgca aggcgatttt ggtcaaagtt
     2401 tcttatacaa acgctctgtt gctgacttga tcggcgaacg gatcatcaac actttatatc
     2461 tatcgatctt gacagttgtc cttacttatt tgattgctgt gcctctgggg atgcttgctg
     2521 gccggtatca aaattcaatc ttggataaga ttgtcgtaat ctacaacttc tttagttttg
     2581 ctgttccttt atttatcttc ggcttgatca tgctatttgt ttttggttat cgtttaggct
     2641 ggttcccaac aagtggatca caaacactag gtttgcaagg tggatttatc actcaatgga
     2701 tagaccgttt gaaatatatt ctgttaccat caattaccca agcatttttg gcgactgccg
     2761 tcacaatcca atatctgcga agtgaagtaa tcgattcgaa atcgctcgat tttgtcagaa
     2821 cagcccgttc caaaggggta ccgacgaata aagtattcag tcgacatatt ttccgaaatg
     2881 cagcgttgcc gatggcttct caaatggggt atgaaatcac ttcgttgatt gctggttctg
     2941 tagttatcga aaagattttt ggttatccgg gagtcggaaa actatttatt gattccatcg
     3001 gccaacggga ctataccgtg atcacatcct tagtattgat tttaggaatc gcgacgctag
     3061 tggggacatt actttcagat atcatcatga gtatcgttga cccgcgtatt cggattcaat
     3121 aaaaccaatc agaaaggaga atggatcgat gagtgaaaaa aacaatacag cagttcaaga
     3181 aagtattcct cctatgggga tacgaatgat tgctcgagaa tttaaaaaag ataaaattgc
     3241 tattttttca ttaggtttgc tagtggtttt attattagtg atctttatcg gtgcgatgct
     3301 gaccgatcaa gacaaagtga tgtatgttag tatcttcgac aaatacgctg caccaggaac
     3361 agtttcgaca caaggacaaa agttcattct gggagcagat gaaggcggac gtgatgtatt
     3421 aggacaattg atcataggtg ctagaaactc tgttacgatc ggatttgcga tcacgatcct
     3481 tacttcgatc atcggggttg gattagggat catctctggc tactacggag ggatcattga
     3541 caacatcttg atgcggatcg ttgattttat catgattcta ccgatcatgc tgatcattat
     3601 cgtcttcgta tcgattattt ccagatacaa tgtctggtcc tttatattca tcatgagtgc
     3661 attttattgg gtagctaaag cccgattatt ccgaagcaag acattatctg aagcaagaag
     3721 agattatgtc agtgcctcaa aaacaatggg aaccaatgat tttaaaatca tgttccgcga
     3781 aatcatgccg aatctaagtt cattaatcat tactaatttg acgatgaact ttgcggcaaa
     3841 catcggtatc gagacaactt tgactttctt aggtttcggc ttgccggcaa atgtacctag
     3901 tttaggtaca ttgatcggtt atgcaagtaa cggagaagtc ttagtgaatc ggcaatggat
     3961 ctggttaccc gcatcaatcc ttattttagt tttgatgttg agtatcaatt atgttggaca
     4021 agcattcaag cgttctgcag atgcgcgcca acggttaggc tagtatagaa gcgaaggggg
     4081 aaataggaat gaagaaaacg atttttggat ttgcagcgct gatgtctgct gtcgttcttg
     4141 cagcatgcgg gggcggaggt acagatacct ctggaaacaa ctctggaggc aacggcaata
     4201 acaaagagga attaactttt ccaattgcaa caaaaaataa tgatgatgca atcgaagacg
     4261 gcgaattaga agctgcggtc gcaacggaca gtcagtttaa aggattgttc cagtgggagt
     4321 tttaccaaga ttcatatgat gcagctttta tggctccttc tcatgaaccg ctatttgcaa
     4381 acgatgacga tttcagaatc acagacgacg gagctgcaac gcttgatcta gatgaagata
     4441 cgaaaaaagc gacaatcaaa gtaaaagaca acgtcaaatg gtcagatggt gaagatgtaa
     4501 cggcggacga tgtgattttc ccttacgaaa tcattgggaa taaagattac actggtatcc
     4561 gttatgacga cactttccgt aacatcgtag gtatggaaga atatcatgat ggaagtgccg
     4621 acacaatctc cggaatcaaa aaaatagacg acaaaacagt tgaaatcgaa tataaagaag
     4681 taaacccaag catgcttcaa tcgggtggtg gtatctggtc atatgcagca ccaaaacata
     4741 cgttagaagg tgtagcaatc aaagatatgg aatcaagcga tcaagtacgt aaaaatccag
     4801 tgacttttgg cccttattac atgagtaata tcgttgcagg agaatcagta gaatacttgc
     4861 caaacgagca ctactggaat ggtacgccta aattgaaaaa gatcacgatg aaatctgtac
     4921 cgatggcaag tgcaactgaa gcattgaact ctaaattata tgatatgatt ttccaaatgc
     4981 caacagatac ttacgatacc tataaagaaa ttgctggcta tacaaactta ggccgtgaac
     5041 aaacttctta tacttatcta ggattcaagt taggtaaatg gaatgcagat acaaagagtg
     5101 tggaatacaa tccagattca aaaatggctg ataaatcatt acgtcaagca atgggctatg
     5161 cattagataa taatgcagta ggtgaacgtt tctatgcagg tttgagaagc aatgcgactt
     5221 cattgattcc tccagtattc cagtcatacc atgatgatga gatgaaagga ttcacacaag
     5281 atctagacaa agcgaaaaaa ctattagatg atgctggcta caaagatgtg gatgacgatg
     5341 gtttaagaga agacaaagat ggaaataaac taacaatcaa ctttgcttcg atgtccggtg
     5401 gggaaacagc gcaaccattg gcagattact atatccaaca atggaaagaa attggtttgg
     5461 atgtccaata cacaacaggt cgtctgatcg aattcaattc tttctatgat aaattagaaa
     5521 acgatgatcc tgaaattgat gtttaccaag ctgcttggaa tacaggaact gatccaaatc
     5581 caagtggact atggggacca aatgctgcgt tcaactacac tcgttatgaa tcagaagaaa
     5641 atacaaaatt gatcaacgat atcagttcaa gcaaagcatt tgatgaagag taccaaaaag
     5701 aagcatatca aaaatggcaa caatatgcat ttgaagaagc atttgcgatt ccaacgttgt
     5761 tccgtaatga agtattgcca gtcaacgacc gtgtcaaaga ctgggattgg tcttatgatg
     5821 taccaaaccc atgggcaaat gtttctgtta cagcagattc acgttcataa tcactaaaaa
     5881 tgaccgaaaa agggcgatgt cctttttcgg ttttttgata tttttctgtt tatttctcta
     5941 atattcaggt caaggcgctg atttcatgac ttctccaata aacggtgttc aaagtccagt
     6001 ttctcggtat taagtcaaat ttatgcaaaa atatggaaag ccattttcac aaaatttttt
     6061 cttcatactc gaaactaaac gaatttttca caacttctcg tcaatcacta ggattattaa
     6121 agctttactt accttttgac cgaaccattt caaatttgac aaaaaaccag tataataagg
     6181 aagtatcgaa aaaatatcca taaaaggaat gtggaaaatg gacaaccagc tgacaaaaga
     6241 actaaaagaa aactataaca tcgtttttca cgatctaaat ttgcttgaac aagcttttac
     6301 ccattcatcc tatgtgaatg agcatcgcaa tctgcaatta tcggataatg agcgacttga
     6361 atttttagga gatgccgttt tagaactgat ggtttctcag tatttattcc gtttatatcc
     6421 tcatttgcca gaaggaaaat tgacaaaaac acgagctgcg atcgttcgcg aagacagctt
     6481 gtcgaaattt gccaaagaat gtcactttga tcaatatgtt ttgttgggca agggagaaga
     6541 gaattcagga ggacgtacgc gaccagcatt gctttgcgac ctattcgaag cttttttagg
     6601 cgcgttatat cttgaccagg gattcgacac gacccatgca ttcatcgaaa aagtgatttt
     6661 tcctaaagtc aaagcaggtg ctttttcaca tgagatggat cataaaacaa aactacaaga
     6721 agtcttgcaa aaatcaggtg atgtatcaat cgaataccga ttgatcaatg aagaaggtcc
     6781 agctcatgaa cgagtatttt ggatcgaagt ttatgtagat gatcaattga ttggcactgg
     6841 acaagggaaa tcgaaaaaat tagcagaaca agctgcggcg gaaaatgcat tggcagcact
     6901 gtaaaagtaa gtgagggaaa gctttgtatt tgaaacgaat tgaaatagcc ggttttaaat
     6961 catttgcaga caagacagtc attgattttg aaaatagcgt cacagcagtg gttggaccaa
     7021 atggcagcgg aaaaagcaat attacagagg ctatccgctg ggtattagga gaacaatctg
     7081 ctaaaagtct gcgaggcgga aaaatgccgg atatcatctt tgccggttcc gatacccgta
     7141 agcagctgaa tattgcagaa gtcacagtga ttttagacaa tagtgatcat tatctgccat
     7201 tggagtacaa tgagatcagt gtaacccgcc gttatcgacg gactggggaa agcgagtttt
     7261 tcatcaataa acaaagctgc agattaaaag atatccaaga attgttctta gattctggat
     7321 taggaaaaga atcgttctca atcatttctc aaggaaaagt agaagcgatt ttcagtagta
     7381 aaccggaaga ccgtcgtggg atctttgaag aagcagctgg tgtcttgaaa tacaagcagc
     7441 ggaaaaagaa agcagaacag aagctttttg aaacagaaga caacctcagc cgcgtccaag
     7501 acatcatcca cgaattggaa gaacagttaa ctcctttggc cgcccagagt gaggcagcaa
     7561 aggagttttt acggctaaaa gaaacactga ctcaaaccga tgtctctttg atggttgctg
     7621 aaatcaagac agcaaaaaaa gattgggaca acaaacaagc acagcttgcg aaattcaatc
     7681 tagaattagg caaactatct gagtcgatcc aagaacaaga atccattttg gcgaagcagc
     7741 gtaaagaaaa tgcgcaagcc gatcgtctga tcgaaaaaaa tcagcaagta ttattagatc
     7801 tttcggaaaa actgaagcag acagaaggac aaaaagatgt tttgcaggag cggacaaagc
     7861 atacacaaaa aagctcgcaa gaatatcaaa cttcattagc agaagcacaa aaaaaggtta
     7921 aacattttga aaagcttcaa gaaagcttga tgaaagcagc agcagaaaaa gaaacagaga
     7981 tccaaaaagc tgaagcaaat ctcataaaaa cacagcaaga attagaaaaa taccaaaaat
     8041 caacgaaaga attattggca gaattaagag atcaatatgt tgatttgatg caagaacaag
     8101 cagctgtagg aaacgaactg aagtatctag aacgtcaata tatacaagaa acagcaaaaa
     8161 gcaagcagac gttagccaag caatcggaag tagaagcatc agtagaccga ttgatcttac
     8221 aaaaagaaga attgacacaa aagcaggcgc agctaaaatc atctttaact gaaacaaaag
     8281 agaagttaga aatgatccaa caaaacggga aaaagttcca agaagcatta gcaaaagaac
     8341 agcctaaaat gtatcaatta atgaatcaag tccagcagct acgtgctcgt aagaaaagcc
     8401 ttcaagaaat ccaagaaaac tattttggtt tttaccaagg ggttcgattg gtcttgcagc
     8461 ataaacagca gctttcgggt attgtcggag cagtggctga actgatcgat gttcctgcag
     8521 atttcacgtt agctatcgaa gcagctttag gtggggcagc ccagcatgtc atcgttgaaa
     8581 atgaaaaaga tgctcggcaa gccatcacgt atttgaaaca gcaaagagga ggaagagcaa
     8641 cattccttcc actgacgaca atcaaaccaa gacaattacc ggctcatata ttgacacaag
     8701 cagcagctgt agaagggttt atagggattg ccagtgaaca agtcagttat cccgatcaaa
     8761 tccaaactgt ggttcaaaac ctgctaggaa cgatcctttt agccaaagac ttaactagtg
     8821 ccaatgcaat cgcgcaaacg atccgctatc aatatcgagt ggtttcctta gaaggtgatg
     8881 tgatgaatgc cggaggatcc atgacaggtg gagcgaataa acgaggaaac caaggcagtt
     8941 tgtttgtaca aaatcaagaa ttgaaacagt tgactagtga atttgaagaa gcagataaac
     9001 aactccaagc acaagaaaaa aaagttcagg agctgcaaca agaaacagct cgtttagccg
     9061 aagaacaaga agttttgcgt acacgaggcg aacaattacg ttttgaagaa caagaagcga
     9121 caaatcagct acaaaacatc ataaatgaac ttgaacgttt tgaaaaagaa aagcaaatct
     9181 caacttttga gacacgtgaa ctgcagcagt ttatcgaaga ttacgaaaag caaacgaacg
     9241 aattgaaaga caaacaaaca gatcttgaat cgcaacgaca aaaaatcgac gaagaaatca
     9301 aatcgttaag tcaagaaagc gaccagatgg aagccagaag agcacaagta caaagtcaaa
     9361 aagcacaaga acaagcagat cttgctgtct tgaaagagca gtttaatcat ctgcagatcc
     9421 agctccgcgg tgcccgagtc caaaaagcag aggcaaccga acgtcaagaa gcgatagaaa
     9481 agcagttggc gacattgaca gcagactttt ctgatcatga agtcacagaa gaaagtttgg
     9541 aaaaacaaat taacgaatta tctgctcagc gagaaacatt aaaagccgag ttggcaaaag
     9601 ccaaagagca gcgagataga aggcaaaagg agatcgataa gcttgaagca gtcttggcag
     9661 agcgtaatcg tgagcaaaaa gcacgactgt cggaacaatc caaattagaa gtccaaaaag
     9721 accgagctga aatgatttta gataatcatt tgacctatct gcagtcagaa tatcagctga
     9781 catttgagaa agcttcccaa gactatcaag aaacaaccga tatcgaagac agtagaacaa
     9841 aggtttcatc cttaaaagaa cagattgaaa aactgggacc agtaaactta aattcaatcg
     9901 aacaatacga acaagtcagt gaaagacata cattcttagc aacacagcga gatgatctgt
     9961 tagcagcgaa aaaccaattg tttgaaacga tggatgagat ggatgatgaa gtgcggacaa
    10021 gattcaaaga agtatttgaa gctatccgtc aagaattcaa aattgtcttt ccaaatatgt
    10081 ttggcggcgg taaagctgaa ttagtactga cggaaccaac tgatttgctg aaaacaggta
    10141 ttgagattga agtacaacct cctggcaaaa aactgcaaag tctaagtttg ctttctggag
    10201 gagaacgagc acttacagcc atcgcgttgc tcttttccat tatccgtgtc tgtcctgttc
    10261 cgttttgtat cttagatgag gtagaagcag cgttagatga agccaacgtg aaacgttttg
    10321 gtcgttactt gagcgatttc caagatgaca cacaatttat cgtagtcaca caccgcaaag
    10381 gaacgatggt cgcagcagat gtactgtacg gagtgacgat gcaggaatcc ggtgtatcaa
    10441 aaatcgtttc tgtacggatg gaagatatca atgaagaggc gaaaatcaac gaaacgaaag
    10501 tgtaggaacg aaaatgatca aattagtagc aatcgatttg gacggtacat tattaaatga
    10561 acaaaagctg atttcagatg agaataaaca agcattagca caagcaaaac agcaaggggt
    10621 caagatcgtt ctatgtacag gacgcccctt agctgcgatg gcgcattatc tgcaagaatt
    10681 aggactggta gatgaaggtg actacagcat cactttcaac ggcggtttgg tacaaaaaaa
    10741 tgatactggt gaaatcatgg aaaagaaagt gatggaagta tcagatatcc aacgcttata
    10801 caaactggct cagcaattgg atctgccact agatgttttg tctgacaatg tggttttaca
    10861 gctgcctagt gccccaagga agcaatcctt gtacaatgtg ttgaataagc tgctgcaatt
    10921 ccaaccagcg gatataagcg atattacaga tgaatttgtg cttaataagg cagtaatcgc
    10981 ttatgaccaa gaagaattag atcctaaaat caaagaaata ccagcagaat tccatgaaat
    11041 gtatgagatc atcaaaacaa gaagtatgct tttggaattt atgccaaaag gtgttacaaa
    11101 agcttatggt atttctttat tggcaaaaga tcttggtttg aaacaagaag aaatcatggc
    11161 gatcggtgat gaggaaaatg atttaccgat gatccaatat gctggactag gcgtagcgat
    11221 ggagaatgct gtgcctttcg tcaaagaagc agcagatttc gtgacagcca gcaatgaaga
    11281 aaatggcgta gccaaagtga tccaaaaatt tgtattagag tagggggagt caaatgggat
    11341 tttttgataa aatcaagcgt gcttttactg gcgaagagaa aacagaagaa gttgaagtac
    11401 aggagaagta cgacaagggt ttagaaaaga cgcgtaaaac atttggtgaa cggatgaatg
    11461 agttgtttgc caattttcga acagtcgatg aagacttttt tgaagaatta gaagaaacac
    11521 ttatcggtgc agatgtaggt tttgaaacag cgatacgaat caccgaagca ttacgtcaag
    11581 aagtgaaact cagaaatgcg aaaaaatcgg cagaagtcca aaatgcgatc atcgagaaac
    11641 ttgtggatct ctatgaagcg gaaggaatca atgaagtaaa tgaactaaat atccaagaag
    11701 acggactaac agttattttg tttgtcggtg ttaatggtgt agggaaaaca acaagtattg
    11761 gtaaacttgc ccatcagttc aaaaacgaag gtaaaaaagt attgatggca gcagcagata
    11821 ccttccgcgc tggagcaatc gaccagttag ttgtctgggg cgaacgcgct ggtgttgaag
    11881 ttgttcgtgg caatgcagga ggcgatccag cagctgtcgt attcgatgct ttggaaagag
    11941 caaagacaga gcaggcagat gttttactag ttgatactgc aggccgactg caaaataaag
    12001 tgaacttgat gaacgaatta gaaaaaataa aacgagtaat ccagcgggaa ttaccagaag
    12061 caccccaaga aacacttctt gtattagatg caaccactgg tcaaaacgcg atggttcaag
    12121 caaaacaatt caaagaaaca acagatgtga ccggtcttgt tttgacaaaa ttagatggta
    12181 cagcaaaggg cggtatcgta cttgctatcc gtaatgaatt gcatttacca gtgaaattgg
    12241 tcggtcttgg tgaaggaatc gatgacttgg agccgtttga tccaaatgat tttgtcgttg
    12301 gattgttcaa aggattacta aaagaagaat aacccatgtc taaaaagcag gagctgtgac
    12361 aaaagttgca gctccttgtt tgaatgattg tttttaacat agctgtgttt ttgatgcctt
    12421 catttttata cttatttgta agcggttctc aaacaatttc gttctttttc ttagactttt
    12481 tgattataat tagattcata tgaattggag gtcacttaat gatgaatttt aagcaattat
    12541 tattgcatgt caatgcgcgc ccttttatcg accaagctcg atttggtata gaacgggaag
    12601 gacagcgggt tgatcttgca ggaaatctag caaaaaccga tcatccagca atctttggcg
    12661 atcgatccta tcacccctat atccaaacag attttagcga aacacaaaca gagatgatca
    12721 cccctgttac cgattctatt cccgaattat ttcagtatct cgctgctgtt tatgatgtga
    12781 ctgctcgttc tataccgaaa gaagagatga tctggccatt aagtatgcca cctgccttac
    12841 cggaaaaaga cgaagagatc attattgcaa aattaaaaaa tttcgaagat gtcttgtatc
    12901 gacgttattt agcaaaagaa tacgggaagc gaaaacagat ggtaagcggt attcatttca
    12961 attttgaatt tggcgatgag ttgctaagaa cattgttcag ccatcaggaa gaatttcaag
    13021 atttttccga attcaaaaca gaactttatt tgaaaacagc gagaaacttt atgcgctatc
    13081 gctggatgat cacttattta ttcggtgctt caccaatgag tgagaagaac tactttttag
    13141 atgaatcaca tccgcaagaa cctgttcgca gtattcgcaa cagtgcgctg ggctatacga
    13201 atcatccaaa tgtgaaagtt tcctatgctt ctatgaaaca gtatttagca gatatcgagc
    13261 gaatgatcga agaagggaaa ctttcggagg agaaagaatt ttatacaccg cttcgtttcc
    13321 gaggcggaaa gaaagtcgca gatttagcaa caacaggtgt tcgttatatt gaattgcgga
    13381 atatcgattt gaatccttat gcaagattgg gtatcaatcc agagcaagtt cggttcttac
    13441 aattattcct gatgtacatg ttatggacag aagaaaaaga agactgtgac caatgggtag
    13501 cagaaggtac gactcgaaat aacaaagtag cactagaaca accatcagat caaacagaat
    13561 ttcatcaaga aggtagagaa attcttgaag gaatgaagca aatgctggtt gaattggatt
    13621 ggttggattc tctttattta gtagaggaag cattgactca aatggatcat cctgaacaaa
    13681 cgttagcagc gaaactctat caagaagcac agctatcgag tcagcaagaa gttgctgtgg
    13741 cattgggaca tcaatattat aaagaaagcc atgaacgtcc ctatcagttg gccggttttc
    13801 gcgaaatgga actttctact cagatattta tgttcgatgc catccaaaaa ggtgtccaag
    13861 tcaaagtatt agatgagtcg gatcaatttt tgcgtctgca attccaagac catgtagaat
    13921 atgtgaaaaa tgcaaacatg acaagtaagg acagctatat cgtcccactt atcatggaaa
    13981 acaaaacagt aacgaaaaaa gtcttaaaag aagctgggtt ccgggtacca ggaggcgcag
    14041 aattttcatc catggaagaa gcggtaaaag cttatccgag atttgccgag caggcatttg
    14101 tgatcaaacc taaatcaacc aattacgggt taggtatcac gattttcaag gaaggagcta
    14161 gtctggaaga ttatcaagca ggtttagcca ttgcttttcg tgaagacagt tcggttttag
    14221 tagaagaatt tatgccggga acggaatatc gtttctttgt gatcgacgga gaagtccaag
    14281 ccatcatgtt gcgagtccct gctaatgtta ttggtgacag tatccgaaca gtgaaagaac
    14341 ttgttgaaga aaaaaatagt gaccctttgc gtggaacgaa tcatcgtgca cctttagaat
    14401 tgatccaatt aggtgaatta gaacaattga tgttgaaaga acaagggttg acgatcgaat
    14461 cagttcccca agccaatcaa attgtttatc taagggagaa ctcaaatatc agtacaggtg
    14521 gtgattcgat cgatatgact gatgaatttt ctgaagctta caaaaaaatc gccgtttctg
    14581 cggtagaagc actaggagct aagattagtg gtatcgattt gattatacca gataaagaaa
    14641 tcgatcctac aactgataaa aaagcatatg ggatcatcga agcaaatttc aatccagcca
    14701 tgcatatgca tgtttatcct tttgcaggaa aaggcagaag attgacgatg aatgtattga
    14761 aattgttata tcctgaagta ttttaaaaga aaaagctttt gacagattgt cctatttgat
    14821 gtcaatctga tcaaaagctt tttttgcatg taaaaattta tgtgtttttt cgttttaaaa
    14881 taaacgatcc cagaagcttt ttttcttttt tggtttttct ttttcagctg gcgaagagtt
    14941 ttcttctggg tatttttttt cgatatcaat cacaggttca tttactgctt cttttgctgt
    15001 taagagcaat ccgaattcct caggatttgc agtgacgtaa tcgttgacta cagtgaaacg
    15061 gctattgttt ttcgttgcta gctggatata ttggttttgg acagaaaggg gtagagcccc
    15121 attcaagagt atggaagcat ctggatgttt tttgagttct tttaaaaagt tttctttgtt
    15181 ttttggttgg atcatttcag caacagtcat actaagatag cagcgttccc tgaatgtccc
    15241 taaatattta tgctgttctt cagggttcac taaaggtgta ccataaagtc ccttatccaa
    15301 atggcttttt acatcatctt gtgccataaa aaataccctc cttcttctct ttgtctttct
    15361 tacgattata gcatattccc aataatacct aaaacatatg aaaaagcggt tgcttttgtt
    15421 caacgattca cttaaaataa gaatgaaaat cattctcaat tagaaaggtg gattggatgc
    15481 gctacggacg actagtagca ttattagtta ttttaagtag tatttcattg ttcatcggag
    15541 tatcgaatgt acaattgtca gaactgctcc agttagatgg gcaaagctta caaatcttgc
    15601 tagtgactcg tttgccacga acgatttgtt tgatccttgt gggagcgacc agtagtattt
    15661 gtgggttgat catgcaacat ttgacccaaa ataaatttgt ttcaccaacg actgcgggca
    15721 cgatggatag tgcgcgttta ggtattttag tggctatgat tttcctacct ggtgcatcct
    15781 tgattgtccg atccttgaca gcattttgct ttgcttttgc aggaacattg ctttttttat
    15841 cccttacgcg attgttcctc cagaaaaatc aattgatgct tccgttgatt ggagtcatgt
    15901 tagggaatat cattggttca gttgctactt tttttgctta tcaattccag ttggtacaaa
    15961 atatgacttc atggcttcaa gggaattttg cgactgtgat acgaggaaat tatgagttat
    16021 tgtatgctgt tgttccattg ttgctcttgg tttggctatt tgcttaccga tttacgatca
    16081 ttggtctggg cgaatcgttt gcagtaaatc tgggaataga ttaccagaag atgcagtttt
    16141 taggcattgg cttagtatca ttggctagtg cagtcatgtt gatcatggta ggaagcattc
    16201 cttttatcgg agtgatcatt ccgaatcttg tctctagaat atatggagat caagtgcaca
    16261 aaacaattgg cgtaactgct ttagcaggta gtttgttctt agtattttgc gatcttgtcg
    16321 ctcgcgtgtt gatttttcct tatgaaatac cagtcagtgt gatcgttgga atcatcggtg
    16381 gggctatctt cctttatctt ctgataaggg gacgacgata tgcttaaaaa aaagccatct
    16441 atttttattg gtatgcttgt tcttgtttta tttgttacag gaatgtactt gacgatccat
    16501 acatatggaa actggcattt tgcatgtgca ttgaggagta agaaaatttt tgcatttatc
    16561 cttgtctcgc tagctgtcag cagtgccaca atcagctttc agacaattac gcataaccag
    16621 tttttgactc cttcagtatt gggacttgag tcgttatatg tccttgtcca aacggtcctt
    16681 ttcttcttta tcggaggagt gacgatgctt cagcagacag gtacagtttt tttcttattg
    16741 aacgttgggc tgatggtcgg gctgagtcta ttgttatttt tctttctttt gaaaaaaatg
    16801 agagcgaatc tttttttatt actgatgtca gggatgattt taggtacgct ttttggtagt
    16861 gtcagtacct ttttacaagt catcatggat ccgaatgaat atgatttgct tcaaggcaga
    16921 ttatttgcaa gctttggcaa tatccagagt cagtatcttt tgcctgcttc tttgatcatt
    16981 atcggctgct ggtacctgtt atttcgatat cgacatgtgc tagacgtttt ccatctcgga
    17041 aatgaacaag cagtaaacct cgggatagac acagatcgtt ttgaattttt gattttatgt
    17101 tgtgtcagtc ttttgacagg gacatcaacg gctcttgtgg gtccagttac ttttttaggg
    17161 tttattgtcg caaatatcag ttatcgtgtc ttttctactt atcgtcatgg tcagctattt
    17221 atcggaagct ttctggtagc tatgctgttt ctgacaggag gacagctcgc agtagagcag
    17281 attttcggct ggaatacaac agtgagtgtc gtcgttgaat ttgtcggagg aatttatttt
    17341 gtcacaaaat tgattcgcga aaggaagagt atcctatgat tgatttacaa aaggtcaata
    17401 aactgtacca tggaaaatca gtggttaaag atgtacagtt aacgattcct gaaaagaagt
    17461 tgactgcttt tatcggtcca aatggtgctg gaaagtcaac ggtgttgtcg atgatcagtc
    17521 gtttgatacc aaaagataca ggtgaaatct atttagacca taacgaagta aaggcttgga
    17581 aatccaatga attagccaaa aaattatcga tcctacgaca atcgaatgaa ataaatgtcc
    17641 aaatcaccgt gaaagaatta gtagaatttg gacgatttcc ttatacgaaa ggaaaattga
    17701 cgacaaaaga cgaggaaatc gttcaggaat cactggaaca cattgggtta gaagaaatag
    17761 cggatcaatc gattgacact ctttcaggcg gtcaactcca acgtgcgtat attgcaatgg
    17821 tgctagcgca agacacggat tatattttac tggatgaacc tttgaacaat ctagatatga
    17881 attttgcggt tcaaatcatg cagattttaa aaaatttagt acaagaaatc gggaaaacaa
    17941 ttgttattgt tcttcatgat atcaactttg ctgcaagtta tgcagacgaa attgttgcaa
    18001 tgaaagatgg acagttattt acgcaaggaa cgacccaaga aatgataaaa aaagatgtat
    18061 tggatgaatt atacgaaatg gacatacgga tttgtgagat tgaaggccgc agattctgtc
    18121 tttatttcta aaactaacat taggagagaa gagatgaaaa aattagtagt aggtatcttg
    18181 attctagcat catttggttt agcagcatgt ggaaatagca acaacaccag tcaggcagac
    18241 acaaaaaaaa gcagcacaca aactgaaact acattgacga taacagatag taatggagac
    18301 caaatcgagg tgccaaataa tccagaaaaa gtcgtcgtat tcgacaatgg ttctttagat
    18361 acgatggatg ctcttggtgt tggagataag gtagttggag cagctacaag cagccttcct
    18421 gagtatcttt catcctataa aaaagtagaa tcagcaggtg gaataaaaga accagactta
    18481 gaaaaaatta atcagcttca gccagatcta atcatcattt ctggccgaca aagagatttt
    18541 caaaaagatc taagtcaaat tgctccgacc attttccttt ctttagatgc gaaaaatcca
    18601 tgggaatctt tccagcaaaa tgtcactgct ttaggggaga tctttggtaa gcaagaagaa
    18661 gctaaaactc aattagagga attatctagt gcaatcgatc agacgaaaaa gaaagcagaa
    18721 gctactgaca aaaaagcact agtcacactt gtgaatgaag ggcaattatc agcctacgga
    18781 tctggttcta gattcggttt catccatgat ttatttggct ttgaacaagc agatgatcaa
    18841 atcgaagcat ccactcacgg tcaaagtgtt tcttatgaat atgtgctgga aaaaaatccg
    18901 gatatcttat ttgtagtaga cagaacaaaa gctattggtg gagacgacag caaagatgat
    18961 atttctgcca atgaactggt agcacaaacg aatgctggta aaaatcagca gatcatctcc
    19021 cttgaaccag atgtctggta tttaagcggg ggcggtttag aatcaatgaa actgatgatc
    19081 gaagatgtga atcaagcatt taaatagcat aaataacaga ggattaaact ctcatactat
    19141 gtaacaataa tgtttcttac attcttaaat agcaagagcc tatcctcaaa gaagttatca
    19201 aaaaccatcc gtaaaatcgt attgatgatt ttacggatgg tttttattta tttgagaaat
    19261 cttttttcaa cgtgaattca ttatctacat catacgggtt gattctagaa tggggagtgg
    19321 caaaaaatag tgtgatccat tttttcagca tcaagatcgc ctcctttgat ttgaatcaaa
    19381 ggtacaaagc gaaacttaga taaaacttaa atttagatat ttttattttg taccgatctc
    19441 ttctttaaac gatgttccat ctgctgcttc tcggcaataa gtccactaga ctagaaaata
    19501 tgagaagcta ttttctgtcc agcgttctta ttactcaaaa caaatcgcag ttgtcacaat
    19561 ctcttctaaa cgatgttcca tctgctgctt ctcggcaata agcccactag actagaaaat
    19621 atgagaagct attttctgtc cagcgttctt attactcaaa gcaaatcgca gttgtcacaa
    19681 tctcttctaa acaatgttca atcagctgac cttcggtatt agctcaaagc actgatttca
    19741 caacctctat ttaggcatgt gtctcagcaa gcggttgata gttgatgatc tgcatttttc
    19801 gataaaaatc ggcgggaagc ttactccacc gttttagtaa ttgatagaaa taactctctt
    19861 ctgctttgat tttatactcg cttaatatca atccaaaact attcgttttt tttgaagcta
    19921 attttttata aatatccaat gctttggttt tatcttcatt gacaaagagt gtttttactt
    19981 caatataaga accctcgata ttttgtgttt cttcgataaa atagatgttt tttatatatt
    20041 ccttcatagt gattcctcca aagaagtctt ttatttgata agtttactct accgaagagt
    20101 tgttaataat ccgtgaacaa aaagtaattc tttttccaat agttgttaaa ggttttttat
    20161 tttttatagt attttttgtt atttaagaaa ggaataaggt taaacaaata acaggttgct
    20221 ttttgggtct ttttccgttc aagaaactga acgaagaaaa gggaaagttg tctcttttgt
    20281 gataagagaa atcataataa acgaatgact tactttccaa taaattgaga acagtcagaa
    20341 ccagacaaat tagttgcatc tgatgaataa agtgattatt ctatgacatg aggtgataca
    20401 gatgaaatta gaagtgacaa aagaagcaca agaagtattg aagaataaat tagaagcaga
    20461 cgatcaactt ttattagata tcgaaaacgg tgacggtcca tttgcagaaa gccaagtcag
    20521 ttgccaattg gatacggcat ttcgattgat cattgtcaaa aaagatacac aaacagatct
    20581 aagtctttat tctgtagtgg ctgatacacc agttggacca atcaaaatca aagacagtgc
    20641 aatactttac atggatgatc catccacact tgctttagaa ccaacttata atagtttaca
    20701 attaaaagga ccaagcggct tgagaaaagg caacttgcaa gttgttcgaa aagactagag
    20761 ggataaaatg ggaaaattgg cgatcatcag cgacctacat gtggacatca ataaattcgg
    20821 cgaatcagag ctgatgcagc tttggcaagt tttgcaaaag cacaagatca ctcggcttca
    20881 tctagccgga gatacggcta ataaagtaga ccgttgccta gctgttgtcg aatttttcgt
    20941 caataaagga ttatctacga catttcattt cggcaatcac gagttggcag atgtgcccaa
    21001 tgaacagatg atggaccatt ttccagaccc acatttttta aatgaacggt atattgcttt
    21061 aaatgagcag accgttcttt tagggatgaa tggctggtac gactatcagt tttccgaatt
    21121 gaaagattac gaggcaatcg tacgaatgaa gcgtttgtat tggtatgatc ggttcatcca
    21181 gcgtgggaaa acagatctcg aggtcaatgc agaaatgctt gcagctgcaa aaaatttgct
    21241 agaccagctg gaagcaaaaa aacttgaggt gattttatcc actcatttcg ttcctaaacg
    21301 ggcatttatc atgtatcaat ctgctcctta tgagcgttgg aacaaattaa atgcattttt
    21361 aggatcagaa tcattcggag aattacttga ccgttattca aatatcaaac aagtagtttt
    21421 tggccatacg catcgtcgct ttgaagataa atccatcggt cacacgattt acagctgcag
    21481 accttttggt tattattatg aatggcagct gacccgagat tttgtcttga aagaacagtt
    21541 gctagacagc tatgatccta tgaaattacg tgtcctcttg cgtgcccatc acccagcttt
    21601 tcaggcctat caaacacaac atttgcaaga ggaatttgaa caagcgatga caatcgttcc
    21661 gtattgagac agcattagta atgtgtaaaa gaaaatacct tgtaaaacat aaggataagt
    21721 aagagcagta gatccattta tcgtggatat gctgcttttt ttgcggaaaa taatcagcga
    21781 atgtttattt gatcggatga tttgtaactc gcagagtact agatgcaagc gtgtattctt
    21841 tttttgaacg ataatcacaa aaaaaaatat aatatacatt ttttatttaa ttataattaa
    21901 tattgattga agtctaggca gtcaagt
//
LOCUS       DAHHZJ010000034        21771 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.035, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000034 DAHHZJ010000000
VERSION     DAHHZJ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.035"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(27..878)
                     /locus_tag="NBL02_001024"
     CDS             complement(27..878)
                     /locus_tag="NBL02_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCD2571729.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            1001..1468
                     /locus_tag="NBL02_001025"
     CDS             1001..1468
                     /locus_tag="NBL02_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HCD2571730.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(1731..2213)
                     /locus_tag="NBL02_001026"
     CDS             complement(1731..2213)
                     /locus_tag="NBL02_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HCD2571731.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(2313..3176)
                     /locus_tag="NBL02_001027"
     CDS             complement(2313..3176)
                     /locus_tag="NBL02_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCD2571732.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            3774..4649
                     /locus_tag="NBL02_001028"
     CDS             3774..4649
                     /locus_tag="NBL02_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571733.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(4794..4946)
                     /locus_tag="NBL02_001029"
                     /pseudo
     CDS             complement(4794..4946)
                     /locus_tag="NBL02_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(5214..6545)
                     /locus_tag="NBL02_001030"
     CDS             complement(5214..6545)
                     /locus_tag="NBL02_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCD2571734.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(6694..7407)
                     /locus_tag="NBL02_001031"
     CDS             complement(6694..7407)
                     /locus_tag="NBL02_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCD2571735.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            7582..9006
                     /locus_tag="NBL02_001032"
     CDS             7582..9006
                     /locus_tag="NBL02_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCD2571736.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            9305..10012
                     /locus_tag="NBL02_001033"
     CDS             9305..10012
                     /locus_tag="NBL02_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HCD2571737.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            9999..10322
                     /locus_tag="NBL02_001034"
     CDS             9999..10322
                     /locus_tag="NBL02_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HCD2571738.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(10411..11448)
                     /locus_tag="NBL02_001035"
     CDS             complement(10411..11448)
                     /locus_tag="NBL02_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HCD2571739.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(11769..12752)
                     /locus_tag="NBL02_001036"
     CDS             complement(11769..12752)
                     /locus_tag="NBL02_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HCD2571740.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(13167..13937)
                     /locus_tag="NBL02_001037"
     CDS             complement(13167..13937)
                     /locus_tag="NBL02_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2571741.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMIFGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(13930..14865)
                     /locus_tag="NBL02_001038"
     CDS             complement(13930..14865)
                     /locus_tag="NBL02_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2571742.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(15181..16830)
                     /locus_tag="NBL02_001039"
     CDS             complement(15181..16830)
                     /locus_tag="NBL02_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCD2571743.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(16947..19550)
                     /locus_tag="NBL02_001040"
     CDS             complement(16947..19550)
                     /locus_tag="NBL02_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCD2571744.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(19562..20611)
                     /locus_tag="NBL02_001041"
     CDS             complement(19562..20611)
                     /locus_tag="NBL02_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCD2571745.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(20637..20951)
                     /locus_tag="NBL02_001042"
     CDS             complement(20637..20951)
                     /locus_tag="NBL02_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HCD2571746.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(20967..21413)
                     /locus_tag="NBL02_001043"
     CDS             complement(20967..21413)
                     /locus_tag="NBL02_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCD2571747.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(21414..>21771)
                     /locus_tag="NBL02_001044"
     CDS             complement(21414..>21771)
                     /locus_tag="NBL02_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCD2571748.1"
                     /translation="IITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
BASE COUNT         6608 a         4450 c         3565 g         7148 t
ORIGIN      
        1 cggctttttc acaacctctt tatacattat tcctgatcca atatttcttt taatgctgct
       61 tcaagagaag ttaatggttt tcccaatact ttttctaaat catcagagga aatagctaac
      121 tgatcctttt taatatcatg ttggatcgct aaaaacatgt cagctgcttc ttgagggaat
      181 ccggcttctt ttagattttc gacaaacgct gcatcacttg cttcaataat ttctaacttt
      241 ttacctgtcg cacgctctaa tgccttggct aattcttcat agtcagttag attgccggat
      301 aattctaaga tttctggaga gtcactttct actagtactt ttgctgcagc ttctgcatat
      361 tcacgtttca atgcccaacc tgtttttcca tttcctgcag cataaacgaa ttgaccagtt
      421 tttaaagcct gcccaatgat aggtaattca ttctctacat accaattatt ccgtaaaaat
      481 gtatgggaaa tacctgaatc cttaatcatt ttttcagtga attggtgatc tggcgctaat
      541 acactgatcg agtgatcggc atctgcaaaa cttgtataag cgatataaga taccccagct
      601 gcttgtgctg ccttcactac attcttatgt tcggcttgac gatttcctgg aacgccagag
      661 acaaaaagca atcgatctat tcctttcaac gcttctttca ttgagtctaa atcatcataa
      721 tcaccaatac gtatattaaa tccagcttct ttcagtgctt ctcctttttc ttcactacgt
      781 actagtacat agatttcatt ttgtggaacc atctttttta agaattccag cgcataacca
      841 ccaaaacctc cagttgctcc tgttactaaa taatgcatat ttgccactcc ctattctttt
      901 tatttgttaa gatttacagc tgtacattta taaattacac ctttatcata aagagtgtgg
      961 taagataagt caagcaaaaa aaatgaaagg atgacaactg atgaagtatt ctctgcaatt
     1021 tagtgatgca atccatgtac ttgcctatat tgaaatgttt caagataccc gatcactttc
     1081 tagtgagatg attgccagta gtatcgaaac aaatgcagca aatgtacgta aaatcatgag
     1141 tcagctcaaa aaatccggtt tgatctttac aaaaaccgga aaaccgcagc cttcccttgc
     1201 caaaagtcca gatcagattt ctttactaga tgtctatcgc agcatcgaag gtaatacaca
     1261 gcttatccaa gtcgatccaa aaacaaatcc aaattgcgtc gttggtgcga atatccaaga
     1321 agctttagaa gaaagctacg ctcggctgca gaaaaaggca gaagaagaaa tggctaagat
     1381 cacacttgat agtctgatac ataaaatagc cgttttggaa aaagaaaaaa gaccagaaaa
     1441 cacaggcctc gtagagagat ttttataatt ctctctactg tgcctgcatt tttctgatcg
     1501 gaatcgtcta cataaattct tttattctcc gggggacaaa agccttgtca tgcccctttt
     1561 tcctctctaa tgctcaggtc aaagcgctga agtcatgacg tcttgataaa cggtgttcaa
     1621 gtagctgacc ctcggcatta gttctgaaaa atgcaaaata tggaaagcta tttcccattt
     1681 ttcctctcta atgctcaggt caaagcgcta ctttcacgac ctctatatta tcatggttta
     1741 ttgactaccg gtatccagat ttctcccata tatttcccgt tggtaactcc catttcgact
     1801 acagcatttg gacccccaac ataggcgtaa tcttctactt ctcttaaaac atgcccaaag
     1861 gccgtattcg tcaaatcatt tcttaattct tcaggcgatt ctgctaaagc gattacaaca
     1921 agatacgttc cttttgggaa tgggatgatt cgggtagtgt ttggaacatc ctgactcgtt
     1981 tgaacaccga catagtacat cattttcccg ttatagggtt cgttgactgt aaagaaataa
     2041 tgattttcag cagcttgttt caatgcatct attgttccat taccgataac tttttgctca
     2101 aatgcttgtt tctctgcagc caaaccgaca aaatccgtat tgctactctt caattccaca
     2161 ccaatacccg tcaccgtaaa atctggtttt tcttcgatcc tatactttgc catttctccc
     2221 accctttcta ttatattaag ttcatcttac ataaaattaa agggaaatga cacgattacg
     2281 ctcttttttc tagggatatt tttatttttt aactattttt ttgctttcac tgcaatccaa
     2341 atctcactgt aaacattact gaaatcctct ccccattttg taaaagagat ctcgggagct
     2401 tcgactaact cataatctga agaaggaagc cattctgcat agattttccc ccaagtatct
     2461 tgtaaagttt ggggaaatgg ccctttatta ggaaaaatgg cccaagtgtg ttcttctata
     2521 gcaagcattt ctaaatcagc gtacggattt tcttttgtcg tagcaaagcc aatcatgtgc
     2581 atcaaactgc ccttttcttc caagcgggct ccatcaaatc cataagaaac gttgattact
     2641 tgatttggat ataaatctcc taacttatgc atctcttccc tttgagtctc cgtgatcgtc
     2701 tgcgctaact tttgtatttc ttgattgact ccttcaaatt tgacaggaac ttttttcgcc
     2761 acacctgcca cataaaactg ttctttcttt tcgatacgaa attccatcga ttttcctcct
     2821 ctgacatcaa taaaaaatgt caagcgtgga aaagattttt gtatgccgta tctgctgatt
     2881 tctgaaggca gatatcctga ccaatcacga aaagctcttg aaaaaccttc gactgtttca
     2941 tatccatatc taaacgccgt ttctgtcact cttgatccat ctaggagatc gcgattagcc
     3001 aaagccaatc tacggttttt gatatagtcc attagcgaca tccctgcaat aaacgtaaag
     3061 atgcgtttta attgatattc agagattcct gtattttcgg ctattatttt ataggaaagt
     3121 ccttctgtca aatgttcttc gatgtaatcg atcaattggt tcagatcttt aagcatattt
     3181 atcttcctcc ttgcttgcct ttttatcata ttatcaaaga aagtcaatta cttgatgaat
     3241 caggaaccaa tttagtcaga tgtagaaaat gttgtatgga tctaaagaaa attctcaagc
     3301 agcgtttcgt aacggataat tggtattatc tgcctatcta tgttttacct ttagaaaaat
     3361 aaattttggg gaagacacat tctaagtaaa caaagcggta aaaatatctt ttattccctt
     3421 agttaatccg cttactacct actgtacaaa gcaaattgtt gttcaaactg ctgatcagaa
     3481 cgctgcttgg atttttcttt gcgatacatc atacctcttt tatcaattgg aacaattatt
     3541 tatttctaac aaaatcactt catttttgtc agataaataa aaaacaaaaa aagttccatc
     3601 tttaaaacac gctattcttt tactttttct cacattctct aagaaaagaa aaggagaatg
     3661 aatttttgac aattcgagca aaagtgatta tcaaatatgt aaaatcagct acttttttct
     3721 taaacgtttc tttatactaa atataaataa gtgtttcatg aaaggagaac tagatgaata
     3781 attggcttga gtattttcct gaaaacgttt tggaacgcgg ttacagctat catctgcacg
     3841 gttttgtccg tcatttgaac tatacaagca agtacttgtc cgcaacagtt tctggcacgg
     3901 aagattataa ggtagtgatc acttgggatg aaaagaccaa catgacttgt gattgtctat
     3961 atgccataga aggaaaaaaa tgcaaacaca tggctgctgt cttattcgct tacgaagaac
     4021 gtccaataaa aaaatccaac tactcacttt ccgaactctc gtcccttgtt tcctccgcta
     4081 gttcctctct tgtacgggag cttttaacag agattcttat agaacatccg caatttatcg
     4141 aacgcttcaa agttaagatg ccatttcatg caataaatta ttcagataaa ttaacaacta
     4201 tcatccataa atacgatcat attatcaaga aaaacaaaaa caggaaaaca gcgaagttca
     4261 tcatggagat gcgaaagttc atccaagaag cagttgaatc gctcattcag caaaatgcct
     4321 atcttcccgc attcgaattg atcaatgaag tgattgctac tctcgaaaca ttctattggg
     4381 agcctgagga tgaacgaacc cttcttctga tagaggactg ttattattta tggaaagaat
     4441 tattagcaga agcacctcat gcagaaaaaa ggcaaatgtt ctcttggttc gtctgccaag
     4501 tcgatcatac tgacgcctct tattcaaaac gctactccat aaaaatcctg aaagaagact
     4561 ttcgggaaaa agaattttct aaccaaaaga aaaaaataga taaaaaaact aaagaaacgg
     4621 taaagaaaga cgatttcaat gaaaaatagc aagtaaacga taacaaccat cctaaatcca
     4681 caagaaatac agcagacctt gctttaaaaa cacgaatcat gcaaaggatg aagtaaagaa
     4741 gacttaacgc gtacccaata ttagtcattt gactattagg tacgcgtttt gttttataag
     4801 tcatgacaaa tttccttcgt tgctgggtag attttccggc attgttgata gattttatga
     4861 tacttttcta cattttgtaa gagaggttcc actgcatctg tatattggat aaaattctga
     4921 caacaacttt caggtgtgtc gaaccactct aatctttaaa gataaatatt aaaataacga
     4981 ttaataatat taacagtctt tatttttcac caccaaaaac ctatctttgc atatactaaa
     5041 tagacgtata tacacagata ggtttgtctt gtgaaaaata atgattatcc ttaccaaaaa
     5101 atgtttgttt ttctacatgt tcgtacttat caaaaaatat gatccttatt gaaattactg
     5161 gctatttata ttccaacgac ttcttgtgtt aatttttgtt atctcatagt aaattatgtt
     5221 tcattttcac ttaatttagt tgtcacgtgt tcgtcaattc gtaaaaacgg taaatatata
     5281 aacacacttg cggctataat taatatttgt aaaacagctg ctcgccagtc tccggctgtt
     5341 gctaaaaatc ccgatataac tggtggcgta gtccaaggaa tttgcactac tacatgatta
     5401 attaatccca cagcagttgc aaaatatgct actgtcaaag aaaaaatcgg tgcaagcaca
     5461 aagggaataa tcagactgat attaaataca ataggcaatc caaaaataat aggttcgctt
     5521 atattgaata tagcgggagt taccgataat ttagcaaagt ccttataatc ttttctacga
     5581 ctgaagataa atattgcaat caataaagca atcgtatttc ccgatccacc cattaccgca
     5641 aaggtatctt taaaggccat attgataata tgtggaattt gctctttgtt tgcataagct
     5701 gccatatttt cttgcatatt ctgaatcaaa aacggatcta acaatgaacc tgatattact
     5761 gcttgatgga taccaaatcc aaaaaataga ttagcaatag atgtaattaa caaaaatcct
     5821 ggtaaaccag ttgtaatttt acttaatggc tctgtaacta tcgtggtaat tagcttattt
     5881 atatccatat taaacaattg aatgattata aatgacaata aagcaaacga ggtaatcgta
     5941 ataatgattg ggattaatac attaaaagac tttaaaacag cgggaggaat atttccggat
     6001 agctgaatct gtagtttctt attattagaa agtttaataa ataattctgt agccgctaat
     6061 ccaacaaata tacctacaaa cattcctgaa gctcctgtat aggatatagg tatcacaccc
     6121 gtcacttcaa cagctttatc cgcattttca ggagtaaaca tcatctgcat aggtgttaat
     6181 acaacaaatg tcgaaatagc aactaatacc gctgcaatat gatttgtata atttctgctt
     6241 gcacacatat gataagatac tgctgctaca actaataatg tagttatgct taatgtacca
     6301 ttggcaattg aagtaccaat ttgttgtaac gtggtcaatg tactagtatc gacaatttcc
     6361 cccatgaatc ctgttggttc taagatcact gaattaataa gtgtcacaaa tcctgctaac
     6421 atcataaatg gcataaatgt agcaaacgca tctcgcaaag ttcttaaatg gatctgattc
     6481 cccaacttag tcgcaactat accaaatttt tcaaatacaa tgtccttcct gctacttttg
     6541 cccatatttt ttctccttta tgcttattgt aatcgattac tttttccacc caaataatac
     6601 atcacttatc aaaaaaagta aatactttta attaaatata tatatctttt ttagtataaa
     6661 aaaataagct ctgaaaaaat aattttcccg agcttatttt tcaacaaaaa cagcttcaaa
     6721 aacaaagtct tgatataaat gtctcgatac cgaatactcg aaaggtttac cagacgatag
     6781 agcaacaact ttgtctatct caatcataaa ttcacctgga tctattttta atatttttgc
     6841 gtcaaattca ttcgcagtat ctcctttgat acgtatgtgc gaagtcttta tttccagacc
     6901 cagttcattt gtgatataag catatacaga tttctttaaa tgttttggtt ccaagcctgg
     6961 tatgactgct aatggcatga atgtttgctc aatcgagtat ggttgacaat caataaaacg
     7021 gcttcgtaca atattatata catagtcttt tacactgata cttaatttat cagctacttt
     7081 tttcgatgga agttcaattg agaaagtatg gatttctgaa tctacgtgtt cttcaccaac
     7141 cacgctaaac aaaccttcgt ttggaccaag aactcgtgag ttatcaattg gtgttctgac
     7201 gacttcagtc ccatacccac ttctacttcg caaaactcct tcggaaacaa gtaagtctac
     7261 gcctttcttt actgttaact tgcttgtatg ataaataact gccaattcat ctcctgtagg
     7321 aagtttctca ccaattttat actctccatt tacaattttt ttctttatat ctaagtaaat
     7381 ttccatatac ttcgcaattt ttttcaatgg tgaccctcca atcaaaaaca tatcttcatc
     7441 tatactttaa cataaataaa ccgataaaat ccacatcaaa attatacaca caataaaaaa
     7501 gatatatata tttaaacaaa actatttact ttaagttgta agcggtgtta tcattaagtg
     7561 gaaacaggag gtaaaaaaat tatgacaact atgaaaaagc taacttttcc agatgatttt
     7621 tggtggggtt ccgcttggtc agctgagcaa gcagaaggaa aaggagaaac aggaaaagca
     7681 gagactatct gggatcgctg gtttaaagaa caaccttacc ggttttatca acgaatcgat
     7741 tcctctatta caaccgatca catccatcaa tataaagaag atatagaact aatgaaaaaa
     7801 actgggcata actcctttcg cgtttctatt tcttgggcac gaatgtttcc aaatggcggt
     7861 accggaaaag taaatcccaa agccatttct ttctatcgaa aattattttc cgaaatgaat
     7921 aataacggaa tcaaagtatt tgccaactta tatcattttg acatgcctgc tactcttcaa
     7981 gataaaggag gatgggaatc acgtgaagta gttgatgctt atgttcatta cgctgaaact
     8041 tgttttaaag aatttggtga cttagtttgt cattggttta ccttcaatga accacttggg
     8101 cctattttag gtacttatct tgaagatttt cattatccaa acctcattga ctttaaacgc
     8161 ggagcgcaag cagcattctt cactatactt gcacacgcaa aagcaataca agtatttaaa
     8221 gaactagctc ttcctagtaa aataggcgtt attctaaatt tgagtcctac ttttcctcgt
     8281 agtcaaaatc ctgctgatgt gaaagcagca gaaatcgctg atttatttta tacgcggagc
     8341 tttttagatc ctatggttaa agggaatttt tctaaaaagc ttatcctgtt attacaagaa
     8401 tataatcagc ttcctgaaga ttatacgaaa gaagatcttg tatgtatcta cgaaaatacc
     8461 gctcaaattc ttggactaaa ctattacgag ccacgtcgcg tcaaatctcg ttcttctatg
     8521 gtggatacat ctagtccatt tttaccagag tggtttttcg aaccacatat catgcctgga
     8581 cgacgaatga acgaataccg gggatgggaa atttacgaaa aaggaattta tgatttgtgc
     8641 atggatatca agaaccatta tggaaacatc gaagcattta tctcggagaa cggaatgggc
     8701 gttgcggatg aagaacgttt catggatgat acagggcaaa taattgatga ttatcggatt
     8761 gagtatatca aagatcatct tgcttactta tggaaggcaa taaatgaagg atgtaacatt
     8821 aaggggtatc atctttggac atttattgat tgctggtcat ggatcaacgc ctataaaaac
     8881 cgatatgggc tggtttcact cgaccttact actcaaaaaa gaacaattaa aaaaagtggc
     8941 gaattttaca agcaattaag tgatacaaat ggatttagat acgatgtaga cttgttagtt
     9001 gaataatatt ctaaaattcc acttgaagtg aacacattag aaaacaagaa taatgagaaa
     9061 taccaataac aaatactttt tcttcatact tcatttatac ttgtcagtat aagagactgt
     9121 gacaaatgta gtgatcctca gaagttaggt ctggaaatct aacttgagaa gattttttta
     9181 aactacgtcc agtctttttt atttgtttaa aattgaagtt gacaagtctt cattaaaaat
     9241 ttagaatatt cacaagtttg acgacaatta tgaaaattta tgtcgtgcta aaggagtttt
     9301 gttattggat gaaaagttag atataaaaac tgcgatcaaa gacacgttac ctaccgtttt
     9361 tggttatatc ggtattggac ttgcatttgg tatcgttggg aaagctgccg ggtttcatcc
     9421 gctggtcgtc acgttgatgt ccctgctgat ctatgctggt tctgcccaat ttatcacggt
     9481 cagcatgctt gctagccata gtccactgct ctccatcgtt ctctcgacct ttctagtcaa
     9541 ttcccgaatg attctgatga gtatgacgat tgctccttat ttcaagaaaa atcggctgct
     9601 tcaaaatctg ctgattggca cattgctgac agatgaaagt ttcgcactgg gaatgaataa
     9661 attgaactat actggacaaa agttaaactt tcgttggatg aatacagcta attggatttc
     9721 ttatttgacg tgggtgggtt cttctcttgt tggcgcactt ttagggaatt ttattactga
     9781 tccgaaaaag tttggtttgg atttcgccat cgtcgctatg tttatcgggt tactttatct
     9841 tcaagtcatt tctgatagaa acacctctaa acgattacag ttgatcctga ttggtctcac
     9901 tttgattctg gtatatgtcg gactgatttt tatcccaagt aacttagtga ttttagttgt
     9961 aacactgatt gggtgcggat tgggggtgtg gatcaaacat gccttctttt gagtttacat
    10021 tgctgactat tttaggttgt actttagcaa cttggctttc taggatccta ccatttgtct
    10081 tactgaaaaa attcgatttg ccgcaaccgt tgcttgagta tttaagtttt gtcccgatcg
    10141 tcattatgtc tgctttatgg ttcagcagtc tatttaccca aaatatcgga catctgccgc
    10201 agatcaatct cgaaaatgct ttggcttcag ttcctacact tttagctgct attctttcaa
    10261 aaagcttatt ggtgatcgtc ttggtaggaa ttctttccct ttcgttgatt cgcttattat
    10321 aaataaaaaa acaagagctt atggcaaatt actgtcatgc gctcttgttt ttatcaaagt
    10381 caaataagaa aaaaatcggc gtattcttgc ttatccgttg aacggaccac taacaacgac
    10441 ttttcctaat atatgtcctg tttctacgtc tttggttgct tgtttcaagt tcttcgcatt
    10501 catcccatct gaatagacct tgctcaatgt actgcggata atcccttcat ttgctaaagc
    10561 agcaatgtgc gctaaagctt ctccttgcgt ttcgatttca tagttatagt ctgttttagc
    10621 gaacatatac tcccagtcga agctgataga ttggtttttc caaacaccta tatctaacgg
    10681 ctctttgatc cctacgatcg ttcccacatg tccaaatggt cggacaaggt gtttgatttg
    10741 atcaaagtag cgggtaatat cgaataatac agcgacatac tctacttgat cgattcctaa
    10801 ttcacgaaaa cttttactta agtcgtgatg ataatcgatt ggatagtcta cacctgtttt
    10861 tttcagccat tcaaaattct tcggacttgc agtcgcgtag acttctaggc cagcccaatg
    10921 cgctagttga ttcaaaatcg aaccgacgcc cccagctccg ttgatcacta agattttttt
    10981 cccgcggttg gcattttctt ctggagtcag accaaatttt tcaaataaca attcataagc
    11041 ggtcaatgag gtcaacggca aggctgcggc ttcttcatct gaaagatttt ttggtgcaag
    11101 agcgacaata cgttcgtcaa ccagttggta ttcttgattg cttcctgctc ttgtcgttgt
    11161 acctgcgtaa aaaacgcgat cgccgacaga aaatttttgc acttcatcac ctacagcaac
    11221 aactttcccg actccatcaa accctaagat acgtaaagca tttcttatcc cgttatcttg
    11281 gcgaagtttc gtatcgacag gattaacaga tactgcgttg acttttacca gaagatcccg
    11341 tgaactgggt acaggtatct gttctttttc atcaataaag ctttttggat catcgatcga
    11401 caacccttca taaaaaccga ctaccttcat taattccttt tctgacatac gaattcctcc
    11461 ttagcttcat caagttggag catccataca gttttcattt caatatatat agtatgctat
    11521 ttgttccact tttattcatc ataattattc tcttacttaa agtaaactat ttgcactttt
    11581 ttggttaaaa aagaggttgc gacaagactt ttgtcacaac ctcttttaaa cggtgttcaa
    11641 tcaactgacc ctcggtattc gctcaaaaaa cgacaaaata tgaggagcta tttttgtttg
    11701 gctgttctta ttacttgatg caggacagct ttttcacgac ctctaattca cggtgttcaa
    11761 gcaagaaatc attgttcact gaaaagcgtt ggatgttctt tttcaaaggt gtctaagatc
    11821 tctcgtactt catcagtaga tttcgtattc atcaactgaa ctcgcaagtc atttgctcct
    11881 ggaaatcctt tgatatagat tttaaagaaa cgatgtagtc ctgtgatgga acgcgggatg
    11941 atttctgaat aatgatcttg cagatctagt tgcagacgaa gtagtccgat cagttctttt
    12001 tctgtatgtt ccctcggttc tttttcaaat gcataaggat tcttgaaaat cccccggcca
    12061 atcatgatgc catctacgcc atatttttcg gccaattctt ctcctgtctg gcgatctggg
    12121 atatctccgt tgatcgtgat cagtgtttgc ggagcgattt ggtcacgtaa agcaatgatt
    12181 ttagggatga cttcccaatg tgcatctact ttactcatct ctttacgcgt acgcaaatgg
    12241 acagaaagat tggctatatc ttgatgaagt aggtgactga tccattcttc catctcagac
    12301 atttccgtat agccgatacg cgtcttgacg ctgacaggca aaccgccagc tttagccgct
    12361 tcaatcaatt gtgccgctac ttctgggcga aggatcaagc cactgccttt tccacgttct
    12421 gctacattag ggactgggca gcccatgttg atatcgattc cttggaagcc catttctgcc
    12481 atagcaatgc tcatttctct aaaaaattct ggcttgtctc cccaaatatg ggcaaccatc
    12541 ggctgttcat cttctgtaaa ggtcaatctt cctcgtacac tgtcttttcc atctggatga
    12601 cagaaactgt ctgagtttgt aaactcagta aagaagacat ccggtgctgc tgcatgcttt
    12661 acgacatggc gaaatacaac atctgttaca tcttccattg gtgctaaaac aaaaaaaggc
    12721 tttggcagct ctttccaaaa attagtagtc atttatttgt tactctccat tctatcaagc
    12781 agtgaagtcg caatctttct caaaaacatt gtcattatac taggaaacaa aaaaagaagc
    12841 aaagtgaaca gcttaaaaga tgtttctgat tgagcaaaaa aagactgtga caaaaatctt
    12901 atcacaattc cttttccgga ataaatggtg ttcaaaagct tgcatctgcg gtaataagtc
    12961 caaccaaacg aaaaatagga ggagctattt ttgtttggct gttcttatta cttgatgcag
    13021 aacagctttt tcacaacctc tccactaaac ggtgttcaat cagctgaccc tcggtattag
    13081 ctcaacaaac gacaaaatat gaggaactat tttcccgttt gtttctctaa tactcaggtc
    13141 aaaacgctga tttcacaacc tcttttctaa cttctcaatc ctcttccttt attgatcaaa
    13201 taccaagtaa cggcgtacaa aacaacgatg aatagcacaa gtacgatcat cgatgttgct
    13261 actggcacat cgattgttcc aaggaatccg taacggaaac cgaagatcat ataaacgatc
    13321 ggattgactt tcgaaacagc ttgccaaaat ggcggcaaca tagagatggc atagaaaacg
    13381 ccacctagat aagttaacgg ctgcaagaca aatgtaggca cgatcgatac atcgtcataa
    13441 gactgagcaa aaatcccgtt gagcagtcct gctaacgaaa acaagattgc cgtcatcaat
    13501 aatgtaatga tgaccatgaa ccatgaatac acatgaagtg gtacaaagaa caaggaaatg
    13561 atcgttacga gagagccgac gagaatactg cgacccactc cgccaatcac aaagccccaa
    13621 atgatcacat gcgtaggaac aggcgctacc aagatttctt cgatgttttt ctggaatttt
    13681 tgagagaaga aggacgaaga aacgttggca tacgaactag tgatagcaga catcataatc
    13741 aaaccaggca cgatgaattc catatatgaa aaaccaccca tgtcaccgat ccgaccaccg
    13801 atcatttttc cgaaaataac aaagtacaag gatgtcgtga tcacaggcgg aaccagtgtt
    13861 tggacccaga tacgcaaata gcggttcgtt tccttgatag ctaaactttt taatgcagta
    13921 aaataaagat taaacatttt tctcctccgt atgattattt tcttctgtga ttttcaaaaa
    13981 cagttcttct aaacgattcg acttattcct catagaaaga actttgattc cttgtcttgt
    14041 cagctgatcg aagattccgt tcaccccttg atttcgttca acttcgacag cgattgtctg
    14101 ttcatcttca aatgtacaag cgtatccttc aatcacaggt ttggtctcat atggcgctaa
    14161 atcaaagata aatgtttcaa attgcagttt ggatagaagc tctttcatac tagtattctc
    14221 gatcagttca cctgattgga tgatcccgat gttgcgacaa agcatttcag cttcttctaa
    14281 atagtgcgtc gtcaaaatga tcgttgtacc attttcgttc aattctcgga gaaactccca
    14341 catctcgcgt ctcaattcaa tatcgactcc tgctgttggt tcatccaaga tcagcaaacg
    14401 tggttcatgc atcaatgccc tagcaatcat cagtcttcgc ttcattcccc cggaaagcat
    14461 acgtgcacgg acatttctct tttcccaaag attggactgc ttcaaatatt tttcgctgcg
    14521 tttcatcgct tctttgcgag agacaccgta gtagcctgct tgattcacga cgatttgctg
    14581 gaccgtttca aagggattga aattgaactc ttgcgggact aatccaatct gttgctttgc
    14641 tcggactaga tccgtatcca gatcataacc gaagacattc acttttccag atgttttatt
    14701 gactaaagaa gtaatgattc cgatcgttgt agacttccca gctccatttg gtcctaatag
    14761 cgcatagaaa tctccttctt tcacggaaag atcgattcct cgcaatgctt cgaccccact
    14821 tccataaacc tttttcaaat tatttatttc aagcgcattt gccatttatt tcctatctcc
    14881 ttatagacat gattctttat tgtatcatgg aatacagcag atgaaaaaac aacttggttc
    14941 cagcgaaaaa tcaggttgct gggctcagga aaatctccta atgaaagaca tcaatcggac
    15001 gaagtcagac aaccttttca gaaaaaacaa atcgaagata aaggattact attcatatgt
    15061 gcaacaaaag gagcgtgaga caaaagtaaa gaacactttt gtctcacgct ctaaacacgt
    15121 ataaacggcg gaagcaaaag ccaagatttt tgatccaata aatactttcg atcacttcgc
    15181 ctatcccaat acatgccact gccgcttttc atcaaataac gcggcttccg tttttgcggt
    15241 ttctactaaa gtctctgggt aatccaaaac tttcagcaag gcaatcgcat ttcgggtcct
    15301 tgaaggtcct tcttttaatt tgaaatcaaa actgattcct tttccttctg ttacttgttc
    15361 ttcaaagtgg acattctcac aataattctt taaaatctct gtcaattcga tatcatgtgt
    15421 ggcgacaaat gctaaagatg gatactccgc aagccaccga acgactgaag cggaagaagc
    15481 aatccgttcg atcgtatttg tcccttttaa aatctcatct acgaagcagt agcagcgttc
    15541 tttggaagcc acttggtcca acaaacgttt gatcgatttg atttctgaaa caaaataact
    15601 gtcgccttca aataaatcat cttcgattgc catggaagtc aacacatgtc caggttgcag
    15661 ggtaaactgt tctgcaatcg ctgtttggat cgtctgtgct agaatacagc tgatggctat
    15721 acttttgaca tacgtagatt ttcctgatgc attggaaccc gtgactaatg tattcttctg
    15781 ccagttgacc ggattcacta ccgcttcttt taacaatgga tgatagatat ctttcgctgt
    15841 cacaccgccc tcagtaaact ctggtcggca agtgatgggc atataagtac ggtaattcaa
    15901 tattgcagcg gctacttcta attctcccag caattcccac agcttgatgg cttcagttga
    15961 atgcttttct aatttagtga gaacgaaatg ataggagata aatggcagca tgaacatgat
    16021 gttgacatat tcaaaaatca actctgcttc actcccactc ttcatacgaa atgaaaaacc
    16081 taatttaggg atgtctctta atggtttagt ttgctgtttc agctcttctt gaagtggtgt
    16141 cggcagtttt gctagctgat tggcggctga aatcgttgaa accaaataac tcatgctatt
    16201 cagctctgtt tctagtgttt gtttcttcac tagataatag accgtattga atactaaccc
    16261 accgattgcc aaatagattc ctagtacttg tcctaaaaga agcaaaccca aacccaccag
    16321 cggaagcaag ccaagaaaaa taaataaacc tacgcgtcca atctttttac tttttccatc
    16381 cgctagatat tgttttgcaa aattatgatc tttttttcct aaacgagcga attgatattg
    16441 gattttttct ctcagttgag gattttcctg ataaaaagca atcagttttt ctaactgctg
    16501 atcttcacca aaatcaaagc tgcgtaaacg ttgatacaac gcttctgaac ctacactaga
    16561 ataggtactg ttgattcctt cgaatacttc gaacatatcc aggtcatacc aagtaagatc
    16621 atcgatttcg ctatcccatg aacggaactt cttctccgtg agccatgctt ccttcaagct
    16681 ttcttcttta tcaaatctcg gttgataagg aattttttcc catttgctac gaactgtttc
    16741 ctttaatttc aaacgtgaaa aaatgtctaa agcaactaat aaaataacag ctgcaataat
    16801 aattaaaacg ataaattctc tttgacccat atccctctcc gttcctttat ttgttagctt
    16861 tagtatagca ctgttgcgct atttgaaaag agggtaaaaa aacacctttc aaaataaaag
    16921 gtgttttttt cttgtatatt acatatttat ctctcattta tcaaaattga caaataatca
    16981 tagggaggaa ttgttgttaa ttcttgttta ttctccagtg cattgacaac tgtcccaagg
    17041 gaaaggatag cctcaggtac tattttttct tgttctgttg gattagctaa gcgaagaaca
    17101 aatccttgtt catgaaaata agaaggataa atcgatgata caattaagtc ttcaggtaat
    17161 tcaatcaatg aaaatttacg aggaacatca agttcatttt gtaatggcca aatcttgtta
    17221 tctaacctgt gaataaatac attcagtttc tgcttttgat aagaaataga ttgttctaac
    17281 cgtttttctg cctgttttgc cactttgaac tcattaaatc ttccttcttc tacattaaat
    17341 ccaaaagtga ctttatactc acctatctct tgagctagcg gtgtttccat catgacatgc
    17401 ccttcatttg tcgtatctcc tgaagctcgc ccaggtcgcc aagcaagatt aggttttcca
    17461 agttcccctg ttgttgaaaa taaggttatg attaattgat tttctatacg ttcatactct
    17521 tttaaaccat ctgcataaaa cgtaaaataa tgatcatttt ctgccacaga gacactctta
    17581 tcgaatattt ctaaattaac aggtttctct accatagttt ctgaccaatt cggaggaatg
    17641 acatcagggg tattttcaat aaatccgttt tgaatttgtg caattgattt ttcagattta
    17701 tctaaaacat taattgctaa gcgcatacga tgcgagtaaa cagtattatg aattttgagt
    17761 gtgccttcaa cgatttgact ttctcccaga ctgaacgata tttcataaga tatttcttcc
    17821 atttcgggtt tttcagataa acgatctttt aaatctttag gaagttgagc ttttccgtaa
    17881 acaactaatg tttcttgtaa agaatcttta tatactttag ctgtttctaa ttttagagat
    17941 agttctgtat ctccttctaa tggtgaataa tcatacgtat cgccatcatt tgctgaatcg
    18001 atcaaatgaa cgaaatcaag atattgtcta tctccaacta tcaaattcaa ttgcccatct
    18061 ttaaaagaaa gtttatattt attgttactt atctcagttt tgtctatctc gattgctgtt
    18121 tctaattttt cattattttt ctcaaattca acaacagtgt atcctagtgc tggcacattt
    18181 acatttattt ttatgtcaag ttcataataa gctggttcat caaaatattc gaaacctgta
    18241 gcaatcatcc gataagcatt tttccgttct ggaaaataaa cttctttttc aatgatatta
    18301 tagggactac ctttgaagtg tattttcttt gaatcagcta ctacatgcac gattttcgat
    18361 ccactataat tctcagtcgt tgtgttgaaa acaaggactt cattcgattt tagctttaat
    18421 gcatcagcca tccgttttac aataaggttt tcgattccgt cagctatctc attagcttct
    18481 ttgattcgat ggaaaatatc ttccgcaaca ttatctgaaa cacagccacc aatactatca
    18541 tgagcctggt tttgcaaaat ctttttccat aaccgacgaa gtaggccttt actgatatgg
    18601 ataccatttc tttctgcaat cagcatgagc ggctcaactc gttttagaat tttttgttcc
    18661 aatttaaaat tttctatttt catttttgtt cttactgagc caatagaacg atgaacacga
    18721 gagtaaaccg gctgacgaag ttcaccttta tattcaggta attctttttt gtttctgata
    18781 acttctacat aagaaggata atctgaaata atattgtgga atttactgta tttattcaac
    18841 ttctctacga cttgatcaaa atttaaaatc attgactttt gatctacacc cgaaggtact
    18901 aaaatatctt tgttttttcc ttgcatatga agcatttcaa ctgcttcatc caacttttct
    18961 tttgcaaatt caggaatatc tttaattgcg tctaaggtca tcattccagt catataacca
    19021 aatggaaaat tcatggcata aacaaattta tctcccaatc ctttccagat aaaataagga
    19081 gatggaacca tcgtttcaaa gtttaatcct cgccaacaca tgaaattatc gatttctact
    19141 tgatgcaaaa gtgtaggcaa attcgcattg aacccaaacg tgtcaggcaa ataccctacc
    19201 atcattggat ctccatattt aaggatcgta tcactgattc caatcattaa gtttcttaaa
    19261 attgattcag catccactaa taaggcatct gtttgtgcat accacggtcc cacgaatagt
    19321 tgtcctgttt tcatcagtcg ttggataacc ttaagctttt gcggattgat ctccaaatac
    19381 tcatcgacga ttgaagtttg tccatctaaa caaaagttta ctttagggtt cctttccaat
    19441 tcatctagaa cttcagtaaa gacctgatca gacaatacga tggcatcttc atttgtaaaa
    19501 taccattctc tgtcccagtg cgtgtgttga attatatgtg catttaccat atcagattct
    19561 cctactttcc ttcttctact gaatttactt gttgttcttt tttatacttg atggatttta
    19621 gtgcattgac cattaacgct gtaaccactc ccccaataac aattgatact aaatataaag
    19681 gtatattcgt aacaaaagga attgccataa aggttgaaag cattgtttct tgagtaacac
    19741 caaaaaatcc atttagacca catgctactg cagtgcccac cacgatgctt ggtattactc
    19801 gtaaaggatc tttcacggca aacgggatcg tcccttcagt aatccccaca aggcccataa
    19861 tcatagctgt ctttgcatta tctcgttccg attcatcata aagttttttg tttaaaattg
    19921 ttgctaaaaa cattccaaat ggcggaatag caattcctaa taacatggct gtactaggtc
    19981 caaagcttcc gcttgaaaaa gaagcaaccc caaaagcata tgcgatttta ttgataggtc
    20041 ctcccatatc aaacgccatc attcctccta aaatagccga catcaaagca gcatttgctc
    20101 ctgacatgcc ttctagccaa ttagtcagtc ctgttgtcat tgcttgaata ggcgtagcta
    20161 gtacatacca taatatgatt ccaacaacta atgtagtaac aaaaggaatc agcataattg
    20221 attttagact aataacttgc atcggtactt ttactttttt caatgcatta gctaaatagc
    20281 cacaagctaa tccagcaacc attcctccaa taaatcccat accaagattt tgacacatca
    20341 tcccagtgat aaaagctggt gctagaccgg gacggtcagc aattgaatat gcgatatagg
    20401 ctgaaatcat cggtacgata aactgtaatc ccgtctgacc aatcattcgc aggactcccc
    20461 agaatgtatc cgtctggtcc caaacacctg tgccaccaaa aatgactgca atagaagtta
    20521 gtaaccctgc tgctacgaca agcgggatca tataggaaac cccactttgg aaatgattta
    20581 gaatttctct tccttgtttc ttgaattcca ttagtaactc ccccacgata tattttttat
    20641 ttgttaatta atgcaattgc ttcatcaatc gtctcttcac catcttttac tacctctgag
    20701 gtccctactg ttaaaatcgg tttaccgtca aagcgacctc gttctgaaac atttgtatct
    20761 actgcaaata tgacaacatc tgcctcggaa acatccgaaa ttgaaagttt gttttctatc
    20821 cccatagccc cctgtgtttc tactttaata tctaaccctt tcttcttcgc aaacttttct
    20881 aaattagctt ttgccatata agtatgggca atccctgcga tacatgctgt tactgctact
    20941 atttttgtca tatgaaattc ctccttttaa aatgatagag cttttaccaa ttctttttta
    21001 tttctcgaat cttttattct tttaacaaac tcgtcatcca tcaatcttcc agataattct
    21061 gctaaaatac gtaaatgatt agtaccttta tcagtgtctt ttattgctaa caaaaagacg
    21121 agatcaactg gttgatcatc taatgcgccc cattcaacag accgctttaa cttggctaca
    21181 gccactgttg attcaattac tgctaggctt ttgccatgag gaatggcgat atttttccca
    21241 attcctgtgg tcatatggtt ttctctgtca aatacgtctt tttgaaattg tttgtggtca
    21301 gacacaaacc cattagtctc taacaatttc gataattttt cgactacact ttttttatca
    21361 gatgcattta aatctatcac tatattattt atattgattt tttcactgat catttataag
    21421 ccttctttca atatataaac taatttttca aacgacgaac tcgtttttaa atttgctaat
    21481 aactcatgat tcgaagcgat ttcattgaat gtttcataaa ttttattcaa ttcaggtttt
    21541 actttttcat taagtgcaaa aaagaaaaca gaatgaacaa gtgtcccatc ccaatcaatt
    21601 ccttctggat taacgtagac acagattact ggctttttta taaaatctac ttcgatatgc
    21661 ggcatagcaa aatcattcat cgctgttgaa gatagttttt ctcttttgat tgctccctct
    21721 agtatccctt ctttcgcgta attttctttt accaattttt tggtaattat a
//
LOCUS       DAHHZJ010000035        21688 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.036, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000035 DAHHZJ010000000
VERSION     DAHHZJ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.036"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            434..598
                     /locus_tag="NBL02_001045"
     CDS             434..598
                     /locus_tag="NBL02_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571749.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKENNEV
                     ISFRRRRKAK"
     gene            595..825
                     /locus_tag="NBL02_001046"
     CDS             595..825
                     /locus_tag="NBL02_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571750.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQQNIERWIDR"
     gene            1010..1555
                     /locus_tag="NBL02_001047"
     CDS             1010..1555
                     /locus_tag="NBL02_001047"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD2571751.1"
                     /translation="MHMHIGEKIYKLRRLNNETMNDLVKQTGVSKSWLSDIESGKKQR
                     VDFNKICAIAKHYKVSLEYFRDDYLDSDEHEDIIPLTGETRVKSVKSLEQSLKYLNNK
                     QEPNLLEIKLKDTDSVPEVWYKGERLDELPKGLVDVSYHWKTDDFTNDDRGANDITIQ
                     YFSSFNDKYPDKKTIGHKRDM"
     gene            1556..1873
                     /locus_tag="NBL02_001048"
     CDS             1556..1873
                     /locus_tag="NBL02_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCD2571752.1"
                     /translation="MKEVRPRDEIDKLYKTKRWRDLRQVVIARDFGMCQECKRRGRNT
                     RGTIIHHIVEAREDLSLFWSVDNLECICVACHNREHPERSGGKKKPKPKSHIVKMYST
                     PER"
     gene            complement(2030..2194)
                     /locus_tag="NBL02_001049"
     CDS             complement(2030..2194)
                     /locus_tag="NBL02_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HCD2571753.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGQEVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            2296..2817
                     /locus_tag="NBL02_001050"
     CDS             2296..2817
                     /locus_tag="NBL02_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCD2571754.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            2804..3277
                     /locus_tag="NBL02_001051"
     CDS             2804..3277
                     /locus_tag="NBL02_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003666010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCD2571755.1"
                     /translation="MTTSNILDMSYTERVDYWQSYLEEQASWGGFLKCPYPELLTTWY
                     AERLIDGSIPASKENIQAAKRHMRDLQRQGTDDFPWIFDEEKGHRPIRYIEKKCKPTE
                     GDFGSFVLQPWQHFIIGSMYGWVHRDTGERRFREALIFVGRKNGKFCRLKIGQNR"
     gene            complement(3284..3442)
                     /locus_tag="NBL02_001052"
     CDS             complement(3284..3442)
                     /locus_tag="NBL02_001052"
                     /inference="COORDINATES: protein motif:HMM:NF024064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HCD2571756.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            3535..4296
                     /locus_tag="NBL02_001053"
     CDS             3535..4296
                     /locus_tag="NBL02_001053"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCD2571757.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            4516..5835
                     /locus_tag="NBL02_001054"
     CDS             4516..5835
                     /locus_tag="NBL02_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003666013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCD2571758.1"
                     /translation="MVAYDDEQGANVYVLANARDQASLLFDKAAEMVKQSPALFKKFG
                     KPKRSSINYAPAFSKMEPRASDSRKLDGLNTHFGIFDEIHEFTNYNLINVIKKSRGTR
                     KQPLIVYITTAGYVLDGPLMSYFEQGVDCLEHLEDDIDERTFYYLAKLDSAEEADDPR
                     LWIKANPNICLMNFVGMLDDYVKDKKDPKEYADWITKQFNLFSDIDELSFVDMPTIKR
                     NNKTIDIETLKGKKCVGGFDLSETEDFTAAVLEFPLETGEVFILQHTWIPQARFDRDN
                     NQERIKAWEKVGDLTIIPGDYVNYEYVLNWFVENSKIYDIVKINYDKAKALRLNKELE
                     NAGFETNEIRQGFLSLGGPMQNFKEMLLDGKVIFNNSKLYRWYLSNVKLVMDRNSNWM
                     PSKQSKSRKIDGFAASLNSHAEVLNMLVNPVGTGKVTYYSISDLMNM"
     gene            5851..7071
                     /locus_tag="NBL02_001055"
     CDS             5851..7071
                     /locus_tag="NBL02_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCD2571759.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDVEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALVKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGSNEGLKVKFASNMDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGNNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTTSSKSVAENG"
     gene            7106..7783
                     /locus_tag="NBL02_001056"
     CDS             7106..7783
                     /locus_tag="NBL02_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCD2571760.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGINCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAEAPQEETLLQKKLKA
                     LNGGKNE"
     gene            7780..8973
                     /locus_tag="NBL02_001057"
     CDS             7780..8973
                     /locus_tag="NBL02_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCD2571761.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRRFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     ITPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSKWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIVNVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTTIANE
                     TPAKV"
     gene            8988..9314
                     /locus_tag="NBL02_001058"
     CDS             8988..9314
                     /locus_tag="NBL02_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCD2571762.1"
                     /translation="MILDPKMDLGEIKNALKIDTDDDDVEVSRAAQAAIAYIRGAIGN
                     DKPSFYKQENETVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKASYL
                     LFVEEE"
     gene            9318..9653
                     /locus_tag="NBL02_001059"
     CDS             9318..9653
                     /locus_tag="NBL02_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCD2571763.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVPTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            9650..10045
                     /locus_tag="NBL02_001060"
     CDS             9650..10045
                     /locus_tag="NBL02_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571764.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEELRQNT
                     PYWDGTKSNGKRGSYMQEHAKNHVVTSSIKNGLVEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            10042..10413
                     /locus_tag="NBL02_001061"
     CDS             10042..10413
                     /locus_tag="NBL02_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571765.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EISGNYEDFASNNPLTIQFNVQVDVWVSTMKEVDAFYFALDEVMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            10429..10989
                     /locus_tag="NBL02_001062"
     CDS             10429..10989
                     /locus_tag="NBL02_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCD2571766.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGANVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLTKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEGMTLQKFKDLVNNKEGE"
     gene            11114..11425
                     /locus_tag="NBL02_001063"
     CDS             11114..11425
                     /locus_tag="NBL02_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571767.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     K"
     gene            11449..11640
                     /locus_tag="NBL02_001064"
     CDS             11449..11640
                     /locus_tag="NBL02_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571768.1"
                     /translation="MDQIYKDLIEAGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            11640..15496
                     /locus_tag="NBL02_001065"
                     /pseudo
     CDS             11640..15496
                     /locus_tag="NBL02_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
     gene            15497..16369
                     /locus_tag="NBL02_001066"
     CDS             15497..16369
                     /locus_tag="NBL02_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HCD2571769.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSESVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            16366..17415
                     /locus_tag="NBL02_001067"
     CDS             16366..17415
                     /locus_tag="NBL02_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571770.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGVDEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            17419..18243
                     /locus_tag="NBL02_001068"
     CDS             17419..18243
                     /locus_tag="NBL02_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571771.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            18261..20321
                     /locus_tag="NBL02_001069"
     CDS             18261..20321
                     /locus_tag="NBL02_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HCD2571772.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDQTNQQIGDLGKLKKMYSNSIDFGGYDYSGNPNL
                     LSKLSYDLIENQNTSAGTLSKGENSFKYNKISAEVEGGVELYYKRRGIANWLPSNKTL
                     VMTVKLRAGADYSPVDGKLILIRYRYVDSGTGKIVLDLPINSNSITQEWKEFSITGTT
                     PTFSPQAYHPWIQFRAQDGVLGEIEMSYDIKIEEGSTATPYQPNLLDDSYWLGKTPLG
                     ENLITNAKFPIITRSNPISGFDISEELIIGETYTVSLKGTKPATKEFHLYYGEANYQQ
                     LQYQATLLPVEGLTDVWSATFTARNTSETTNLLRVALWQKPNSATYDTVQIDWLKIEK
                     GDTRTPNISQFKYFGEGLKDSNNPNDYSWDVTPEYTEKSLNNTVSLTEPQSIEGLKNF
                     EDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNGKSSGGN
                     DSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAI
                     TYTI"
     gene            20338..20487
                     /locus_tag="NBL02_001070"
     CDS             20338..20487
                     /locus_tag="NBL02_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571773.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            20484..20930
                     /locus_tag="NBL02_001071"
     CDS             20484..20930
                     /locus_tag="NBL02_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571774.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            20932..21069
                     /locus_tag="NBL02_001072"
     CDS             20932..21069
                     /locus_tag="NBL02_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HCD2571775.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADRIISKEESA
                     S"
     gene            21104..21346
                     /locus_tag="NBL02_001073"
     CDS             21104..21346
                     /locus_tag="NBL02_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HCD2571776.1"
                     /translation="MKEEALQDVVERLVRIETKLDNYESLREKADSAKDLADKAYSVA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            21362..21559
                     /locus_tag="NBL02_001074"
     CDS             21362..21559
                     /locus_tag="NBL02_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCD2571777.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            21570..>21688
                     /locus_tag="NBL02_001075"
     CDS             21570..>21688
                     /locus_tag="NBL02_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCD2571778.1"
                     /translation="MKKKIILSLSLIMALFLLPINGFAYTINNEFNLGVNEGSS"
BASE COUNT         7509 a         3690 c         4833 g         5656 t
ORIGIN      
        1 aactccttct aaattgttct tattatctat catccgttgc tgtctattgt ttttttaatt
       61 attcacacga taactaaagg tgagcgaaga gaaatgattc cattaataat ttcaattttt
      121 gcgctttgtc ttaatgtcta tatgattgga tttaaaaatg ggcaaaataa aagatagtag
      181 taactaagaa agaaattttt atattgtcac tgtggcggaa agggtagacg ctaagcatgt
      241 gtgctaggtc aatgcttcgg caactatgca atgttcgatt cattgccagt gactttggca
      301 accgaggcat cggcggttta aaaatatagg ggtgcgcaat ttcgtacgcg ttttgtgcat
      361 cgtgtaggtt gctattacat attagatcac tcgttgagtg gtctttttat tttacataaa
      421 ggaggtaaca acgatgtata gaccacaata cttagaacag aagtatgaag taatcactgt
      481 gcaaaacggt aacggtgaga tagtacgaaa gtatagaaga ccaataaaga gcgatacata
      541 taaacgaaag gaaaacaatg aagttatttc ttttcgcaga aggagaaaag ccaaatgaga
      601 aactactggt atgtatcgct aactaatgaa tatcctcgaa ccattgatga ttgttcagtg
      661 cgtgttgtgc gttctgtaca aatcaaaggg aagtactcta ttgtcgaaat gctaagagaa
      721 gctacaccaa acgaagtgga taaatgcaag ctgatatatt gcggtcatgg ctattggaaa
      781 gacgagtata tccaacaaaa cattgagagg tggatagata gatgagttac ctcgaacatt
      841 tgaaacgttg ctacatgcat tctaagaata aacttcctga cagctacact gtagatgatg
      901 ttgctataca tgtattgaag actgagagcc atagcagtcc tgatggtagc agtaaggagc
      961 agacgctcgc gtggttcaag ttctttaagt ggattaagga ggaagagtaa tgcacatgca
     1021 cataggagaa aagatatata aattgagacg gcttaataac gaaacaatga acgacttagt
     1081 taaacaaact ggagtgtcaa aatcatggtt atctgatatt gagtctggaa aaaagcaacg
     1141 cgttgatttt aataagatat gtgcaatagc caaacattat aaagttagct tagaatactt
     1201 tcgggatgat tatttggata gtgatgaaca cgaggatatt attcctttga caggcgaaac
     1261 aagagttaaa tcagtgaaat cgcttgaaca aagcctaaag tatctgaata acaaacaaga
     1321 acctaacctt ctagagatca aactaaaaga tactgactca gtaccagagg tttggtacaa
     1381 gggtgagagg ttggatgaat tgcctaaagg attagtagat gtctcgtatc attggaagac
     1441 tgatgatttt actaatgatg atagaggagc gaacgacatc acgattcaat acttttctag
     1501 ctttaatgat aagtatccag ataagaaaac aatcggacac aagagagata tgtaaatgaa
     1561 agaagttaga cctagagacg aaatagacaa actatacaag accaaacgat ggcgagacct
     1621 aaggcaagta gtaatagcta gggacttcgg catgtgccaa gagtgcaagc gtcgagggcg
     1681 gaacacaagg ggcacgatca tccatcacat agtcgaggcg agggaagacc tgtcactgtt
     1741 ctggtccgta gataaccttg aatgtatctg tgtagcttgt cacaacagag agcatccaga
     1801 gagatcaggc gggaagaaga aaccaaaacc taaatcacat atcgttaaaa tgtattcaac
     1861 tcctgaaaga taagtttgca gcgaaatgaa ggtagccccc ctactctaaa agattaaaga
     1921 gtaaggcttg agaagaacgg tgctgtcctt ccttcgtaaa aagaccgctt ttcaagtttt
     1981 ttggagaaaa aggaaaaagc cgaccaattt aagccggctt tggacgaagc tatttcttag
     2041 tccatttgtt tcctttttga gaagtagggg gtaatcggtc gcctggatca atttttactt
     2101 cttgtccgcc ttggacattt ccaccacgag gacccacttc tttgtaggtt ccttttggtt
     2161 tgttgtcttc gccgggttta tagagttctc ccataggaat ccctccttaa caaatttcgg
     2221 cacagcactg ccgataacct aattataagg attgtgataa cgatttcaat ctatcttttg
     2281 aaagaaggtg atattatgcc gcaaccagcg aagagtgcaa aattacaatt attaaacgga
     2341 aacccaaata agaagaatac cgaagaactc cgcaagcgag cggccgcaga agacaaatta
     2401 aaaatggcta ctgacaaaat caaaccgccg ttatggctag attcgctagg aaaggatacc
     2461 tttgagttta tcgccgatga attgctgtct gtggatttaa tcagtaatcc ggacgtccat
     2521 acaatggctc tatactccaa ttggtattcg caatacgttt ctttagaaaa acagcttcga
     2581 aaactacaac gagagtacaa gttgaactat gcgcttgcga aaaaggaggc agaggcgaga
     2641 ggtgagccgt ttaatgaacc taatgaatta attggtaatc cgctctctcg gcagatggat
     2701 acagcgtctc ggaatctccg ttcttttggc gctgatttag gactatcacc agcagccaga
     2761 gctaagttag ctattaagat ggctgatgat ggtggtgatg acgatgacga cttctaatat
     2821 tttggatatg tcttacacag aacgtgtgga ctattggcaa agctatcttg aggagcaagc
     2881 ttcttggggt ggctttttaa aatgtccata tccggaattg ttaactactt ggtatgcgga
     2941 acgattaatc gatggaagca taccagccag caaagaaaat attcaagctg ctaaacggca
     3001 tatgcgtgat ttgcagcgcc aaggaacaga tgattttcct tggatctttg acgaagaaaa
     3061 aggtcaccgg cctattagat atatcgaaaa aaaatgtaaa ccaactgaag gcgactttgg
     3121 ttcgtttgtt ttgcaacctt ggcagcattt cataattgga tccatgtacg gatgggtaca
     3181 tcgtgataca ggagagcgtc gcttccgcga ggctcttatt tttgttggac gtaaaaacgg
     3241 taagttttgc cgtttaaaaa tcgggcaaaa tcggtgaaaa cctttatttt tggctttctt
     3301 ttagtaataa ttcgatagct ttatcaagca atttagacat aggaaccata gtttcttccg
     3361 acattttttt taatccttcg tataaatctt tatcaattgc gttagaaatt ctaattctgt
     3421 tttttagtcc gtatttgtcc atgtgtgaca ctccttttta ttttactata acatccgatt
     3481 ccaccgcttg caactaccat caattgatgg tagtatataa aagaggtgag tagattgaaa
     3541 aaatacatta tttacaaaca cttaaataaa ataaatggca aaatttatat cggtgtcaca
     3601 aatgatattg gcagaaggtg gaggagcggt gggattgaat ataaaccacc aaagaacgaa
     3661 acccaacacc acagaagttt ttggaatgca atacaaaaat acgggtggga aaattttgat
     3721 catttgatta ttgaagaaga cttaacgatg aaagaagctt ttgagaaaga aaagttttat
     3781 attgaacttt atgactcaac taataagaaa aaaggttata acatcgcaaa aggcggaaat
     3841 ggcggaataa tatataaagt tcaccccaaa ggaatgctag ggaaaaaaca atctaaagaa
     3901 ttttcttcaa atcacagtaa atgggcaaag aaccataaga ataattgtat gacaaacggt
     3961 gatgttgtat ggggtgttac tcacgaacac ccaaaaggca tgttgggaaa gcaccacagc
     4021 aaagaatcaa ttatgaaaaa gaaagcatat agtggtgaaa atgctgtaac ctcaaaacca
     4081 attgtcgcaa ttgaacggga tggaactaaa agagaatttc acagcgctaa gctttgcatg
     4141 gcttactatt cgattagtac atgtgttttt taccgacttt taaaagatgg cgccccttat
     4201 gtgataaatc ctaaagcgaa ctacagaaat aaagaaaaaa tattagctat cgaaggaatt
     4261 atgttcaaac ataaagaaga taccgaggta agttagtaga ttgcgaaagg ttactaacta
     4321 ccgtaacgcg taggagttga ataaatataa tgctcccaag agtgtccgac agcagataat
     4381 ttgctgaaaa tgtacgctaa actgggccag aactgactga ccgatgaaaa tgagggtgac
     4441 ctccagagtg cgagataaaa agctcgcaga taataacaat cgaaaacatc actaatttca
     4501 ggcctttcca catacatggt cgcttatgat gatgaacaag gcgccaacgt ttacgtattg
     4561 gcaaatgctc gtgatcaagc aagcttgttg tttgataagg ccgcagaaat ggtcaaacaa
     4621 tcgccggcgc tctttaagaa atttggtaag cctaaacgat caagtattaa ttatgctccc
     4681 gccttttcta aaatggaacc acgcgcctca gatagccgga aattggatgg gctaaacact
     4741 cactttggta tttttgacga gatccacgag tttacgaatt acaatctgat caacgttatc
     4801 aagaaatcaa gaggaaccag aaaacagcct ctgatagttt atatcacaac tgctggatat
     4861 gtattagatg gtccgttgat gtcttatttt gagcaaggtg tggactgttt ggaacatttg
     4921 gaagatgaca tcgatgaacg gactttctat tatctggcaa aacttgacag tgcggaagag
     4981 gctgatgacc caagattatg gatcaaagcc aatccgaata tttgtctaat gaattttgtt
     5041 ggcatgctag atgactatgt taaggataaa aaagatccaa aagaatatgc tgactggatt
     5101 accaagcaat ttaacttgtt ttccgatatc gatgagctgt catttgtcga tatgcctacc
     5161 attaaacgaa acaataaaac catcgatatt gaaacgctca aaggtaagaa gtgtgtcggt
     5221 ggttttgact tgtccgaaac ggaagacttt accgcagccg ttttagaatt tccgcttgaa
     5281 acaggcgagg tattcatttt gcaacacaca tggatcccac aagctagatt tgatcgagat
     5341 aacaatcaag agcgtatcaa agcgtgggag aaggtgggag atctaacgat tattcctggt
     5401 gattacgtca attatgaata cgtcttaaat tggtttgtag aaaattcgaa aatctatgac
     5461 attgtaaaaa tcaattatga caaggccaag gcgctacgat taaataaaga actagaaaat
     5521 gcaggatttg aaaccaacga gattcggcaa gggtttctat cattaggtgg gccaatgcaa
     5581 aacttcaagg aaatgctatt ggacggtaag gtgattttca acaattccaa gctttaccga
     5641 tggtatctat ccaacgtcaa gctggtgatg gatcgcaact caaactggat gccgtctaag
     5701 cagtccaaga gtagaaaaat agatggtttt gcagcaagtt tgaacagcca cgccgaagtg
     5761 ttgaatatgt tggttaatcc tgtcggaacc gggaaagtaa cctattactc gatttccgat
     5821 ttaatgaata tgtaagaaag gtgtggagga atgagtattt tagatcgttt gcgttctttt
     5881 ggccgagcga agccgaaagc gagcaaacaa gagtattttt tgaatgaccc gggattgata
     5941 ccgtatttag tcggaaaaga tgaaatatca gaagggattt tttccgtaat tagccgtgta
     6001 tcgaacgttt ttgcgtctct ccctctcaaa atgatagatg tggagtttgg ccaaccggac
     6061 gactgtcctg catacaactt gttgagcgaa ggccctcgat attttacaaa gtttgatttt
     6121 ttccgggacg tggaagtttt gagaaactac caagggaatg cgtatgtgca gattttccga
     6181 aatatcaatg gagaagtagc agatatggcg ttagtaaaac ctggtgcttg ccatccagtg
     6241 attgatatgg atagcgggga gctttactac caagtaactg cgactgacaa aggcagttac
     6301 aagcaagtta tctatgtaca ttacatggaa atgcttcact ttaaacaacc gaggtttggc
     6361 ggcttggaag gtacagaccc cacaaaagta ttaacgaata ccctcggata tgatcgagaa
     6421 gtccgaaaaa tctctttaag tcagcttaaa ggaagtaatg aagggctaaa agttaagttt
     6481 gctagcaata tggatgaaga agctaaaaaa gctacagtta aaaacattgc tgatttttat
     6541 cgacaaaacg gtggactact tgtggaagaa aacggtgtag aaatcgaacg tttacaacga
     6601 gagctggtag acagcaagct tttagatact gataaagtat ctcgctccag aatcgctatg
     6661 gtctacaatg tgccggagca tttcatcggg aataaccagt cgagttactc ctctcaggaa
     6721 cagctcaata tggaattttt gacgtacaat ctagtaccga ccgttaatca atatgaagcg
     6781 gaactaaata agaaaacact atcgagaacc gaaaaagcaa aaggttatcg atataagttt
     6841 aatgtcgcaa gtttgctaag agctgacact caggcaaggg gacaattcta tcaaatcatg
     6901 cgacgaggcg gagcatactc agcgaatgat gtccgaagat ttgaggactt gcaaccgata
     6961 aataaaaccg gtatggatga ttaccatatt tccggagacc tatatccaat cgatatggat
     7021 ccaacattaa gaaaaacaac ctcgtctaaa agcgtagccg aaaacggtta ggttttttta
     7081 gtttgcaccg aagggaggtg gaaaaatgaa aaaagtgacg ttaagcggcg atgtcgtgga
     7141 taacgatacc gcgtggcttt atgactggtt tgggatcaat tgtatctcac cagggaaaat
     7201 ttctgccgct cttacagaag cagcggggga tgaagtagaa cttgatatct catcgaacgg
     7261 tggggatgtc ctagcggcaa gcgaaatata taccgctatc cgtgcctatc cgggaaaggt
     7321 atccggaaat gttgtgagta ttgcagcaag cgctgcaagt gtaatcgctt gcgcttgcga
     7381 accgcttaga atctcaccta cggcacacat catgattcat aacgcatggg tgaccactag
     7441 tggcaacgct gaggaattaa aagccaatgc agaaatgtta agcagtgtgg atgagtctat
     7501 tgttaatgct tacgagatca aaacaggact cgatcggaaa aaacttgctg atttaatggc
     7561 gaaagatact tggttaaatg ctcaaacagc agtagcagaa gggtttgcag atgaaattat
     7621 gtttgcagaa gcaccagtaa cggtactcaa tgcctctcaa ccggttattc caaaaaacgc
     7681 agtaactaag ttgaaaaatt taatactcaa agcggaagca ccgcaagaag aaacactctt
     7741 acagaaaaaa ctaaaagcct taaatggagg gaaaaacgaa tgaatttaga acaattaaaa
     7801 aatgcgtggg tcgaggcggg aagtaaagtc tctgacttaa atgcacaact caatgcagca
     7861 ttggttgacg atgaaaaaac agaagaagat gtagtaagtt tgcaagcaca agtaaaagca
     7921 gcacgggcta aacgggacgg attgaaagag caagtggcaa atatggaagc cgaacaagtc
     7981 ttaaacgtca aaaaagaacc attagataaa aaagatgaaa acttgaaaaa caagtttatc
     8041 aaagacttta aagcgatggt caatggtgat cctgctatta tggctacttt gacatctaat
     8101 acggatgaat ctggtaatgc tatcggattg actattcctg tagatgtgca aacgactatt
     8161 catactttgg ttcgtcggtt tgactctttg caagaatacg taaacgttga aaaagtgacc
     8221 actaccagcg gttctcgcgt ttatgaaaaa tggtctgata ttacaccgtt gactgctttg
     8281 gatactgaag acggtgaaat cccagcaaat gatgatcctg cactttactt gatcaaatac
     8341 ttgatcaaac gctatgcagg tatttccaca gtaactaaca gcttgctaaa agatactgcc
     8401 gaaaacattt tggcatggtt gtctaaatgg atcgcgaaaa aagtagttgt tactcgcaat
     8461 acaaaaatct tggcagctat tgatggaatc aaagcggcac aaaagaaaga tgttacagat
     8521 gttgatggaa ttaaagatat cgtaaacgtc caacttgatc cagctatcga agctacatct
     8581 atgtttatta caaaccaaga tggcttcaat gttttagata aagtgaaacg tgctgatgga
     8641 tcttacttgt tacaaaaaga cgtagcttct gcaactggat ataccttctt aggtaaacca
     8701 atcaagaaaa ttgcttctcg tttcttgcca aacaaaggta cgcaagctac tcctaaatat
     8761 ccactgtaca ttggtgatct gaaagaagcc gttacattgt atgatcgcga aaacatgagc
     8821 ttgctgacaa cgaatattgg tggtggagct tttgaaacag acaccactaa agtacgcgtc
     8881 atcgatcgct tcgatgtgca actagttgat gatgaagcgg ttgttttggc tacttttaca
     8941 actattgcga acgagacacc ggcgaaagtt taaggagctg attccttatg attcttgatc
     9001 ctaaaatgga tttaggcgaa atcaaaaacg cactaaagat tgataccgat gatgacgatg
     9061 tggaagtaag ccgtgcggca caagctgcaa ttgcatacat tagaggggct atcgggaatg
     9121 ataagccctc tttttataag caagaaaatg aaacggttga tctgattaat ttagctatcc
     9181 tgcaattagc agatcattat tacaacgctc gatcagcaac cgtgagtggg aacttgcgag
     9241 agtacgattt aggttttaca agcctaatct tgcaactcaa agcaagttat ttgctttttg
     9301 tggaggagga gtagcgtatg ccccttatcc aaacaggaaa tttaaatcaa cgcatcaagt
     9361 ttgtccgaga tacgacggtt aaggatgagg acgggcaagt tgtcccgact tctacaacca
     9421 ttcttacttg ctgggcaagt gtgcagacac aacgcctgaa cgatattaag acgtcgattg
     9481 gtacggcttt ggaaggaaca ctgacgttca ttatccgcta ccaacaaaaa tcagagctga
     9541 ccaatgatat gaaagtgcgt tggaatggaa aaacgtttga aatcattacg attacgaaag
     9601 gcgagtttgc gaaggacttc acgacaatca ttgcaaaaga ggtttcaaaa tgagtgtaga
     9661 agtcgatgca accgaagtgt acaaagcgct tagggaagta aaagcaaacg ttcaacgagt
     9721 ggaaagccca gcacttagaa aagctgggga gtacgctcaa gaagagttac gacaaaacac
     9781 accttactgg gatggaacga agtcaaacgg taaacgtggt tcgtatatgc aagaacatgc
     9841 taagaaccat gtggttacaa gctcgataaa aaacggattg gtagaagtcg gctatgacaa
     9901 agatgtttct tggcggatgc actttatcga gttcggaaca atcaaacaac gtccaaaagg
     9961 tttcgtacaa aaaacacaaa agcaaatcga aaaacaagta acacaaatca ttgctgacga
    10021 agtaaaaagg aggctaggac tttgaaaacg gcagtatcac aagtctattc aattctgaat
    10081 agcaatgaaa aaacaaagaa cattgatttt tacaccaata gtgttccgga atcagctcaa
    10141 acagtaccta gccttccagt tggcagaatt acagagatat ccggcaacta tgaagatttt
    10201 gcaagcaaca atcctttgac cattcaattt aacgtacagg tagatgtatg ggtgtcaacc
    10261 atgaaagagg ttgatgcctt ttattttgcc cttgatgagg ttatgagggg gaatggttgg
    10321 caatgcgcat acacggaaca aacagatgac gaggacttgg aaggtgcaaa gcggattatc
    10381 aaacgatatg tagcaaatat ttcactaaat taaaaggaga gaaaatagat ggcaacagta
    10441 ggattcgaga gcgtcatttt tggcgtaaaa acaggtgcag gcggcactct aaaagaatta
    10501 gtagcagata agtcgaaagg cggagcgatc gaagctaaaa ttactggatt aggcgcaact
    10561 tctaacacaa catacgcttc aaacgtaccg ttcttcattg caagtaaagg ggtttcgtcg
    10621 ccaaaagtta cgcttgacgt ggcagactta atggataacg gcatttacag cgaaatcatt
    10681 ggtgctaaaa ccgtggatgg tgcaaatgta attggttcag aaactgaagc gccttacgtg
    10741 tcggtagtca tggttacagc gaacaaagaa ggaaaacgct tgttcatggg attgacaaaa
    10801 ggaaaattca gtcatccaga tatcgacatg aaaacagctg aagacaaagg ggtagaattg
    10861 caaaccgatt ccatcgaagg ggaattcatt tctgatgaac gtggctatgt atacttaaca
    10921 gccgtagaat cagaaggaat gaccttacaa aaattcaagg acttggtaaa taacaaagag
    10981 ggggagtagt taaccctgca tctacaccaa cgacagatac agggacacca aaagaaccag
    11041 aaccaaaaat tgatacacaa ggttagccat ttttggctag ccttattttt tgtaaaaaca
    11101 aggaggaaaa caaatgattg aattgcaatt gaaacttgac ggaaagaaaa aaacattcaa
    11161 acaacaagat atttccgcac gtgcaatgcg tgagtgtatc aaattttacg agaaagcgga
    11221 aaaagcagac ctaactgatt tagaagcgat tgattcaatg attgcaatta cagcagatat
    11281 tttccaagat ccagcagtta catttgatgc tattttagac ggtttgactg cgagcgagtt
    11341 agtaccggca ttagaaagtg tttttgaaca aataaatgaa ctgggaaaca atgaaaaaaa
    11401 gcagacggcg agcaaaaaga aataagtttt tctgaagcta ggaaagcaat ggatcaaatc
    11461 tacaaagatt taatcgaagc aggttggacg atgagagatg tggacgaagc cgactatcat
    11521 tatttgttac acctttttgg agaagtggag agtggcgaag aatatgtaga tggtgctgat
    11581 ttcatcaaac aatttttatc ggctgaagac ttagtaaaac ttgaggaagg aggtaaataa
    11641 tggcaggaaa aggacaaccg gcaggaaata tcaagctagg gattagttta gatagcacta
    11701 gttttggtaa cacgctggac gaaatcaatg cgaaagtcaa acaagctgag tcgaatatgc
    11761 gtgccaatct aaaggcttat gattcagcag gacgttcata cgaagcactt agtcaaaaga
    11821 cgaaagactt gtctacggtt atggaagggc aaaacgccaa agtaagagaa ttaacaaagc
    11881 gccgtgatga agcgattagc aagtatggcg aggaatcgaa acaagttgct aaccttaaca
    11941 cacagataaa caatgctacc gcaaaatata atgcttacag tcgccagttg aacgacacaa
    12001 aaaaagaatt ggtgtattcc aaaacagccg tcaatgattt atctaatgaa atcaaagaaa
    12061 atgaacgaca aatgaacgcc gaagtaaaag cgttgaaagc cgctggtgat gaatctggtg
    12121 cttttgaagc aaaacaaaaa gggctagcca aacaaacgga attatccgag aaagctatcg
    12181 aagaacagcg caaagttgtg aaactgatgg ctgatgagtt tggcgattca gcaaatgaaa
    12241 ccgaagatgc aaaaagggca ttagaaaagt tagaacgaca aagccaaata tctagcaggc
    12301 aattagaagc actcaaaagc tccagcgatc aatcaggaaa agaaatagaa gattttggcg
    12361 acaagtccac aaggtcagct aggaaactgg acggactaaa agacaaatta agctcgctaa
    12421 aaagcgcatt ttcgtttggt gcagttgctg gattagcgca taacgctatt agcagtgtag
    12481 taagtggcgt gcaaggtttg gttggcgaag cagtaaacgc atcggattca ttgatgaagt
    12541 tttccaaaac catggagttt gctaactttg ggaagtcaca gatagaaagc tcgaaaaaag
    12601 aaatgaaaga ctacgccgat aagacggttt atggtttaga agaaattctg aacacaaccg
    12661 cacaattggc atctaatggg attcctaact atacagaact aaccaaggcg gcaggtaact
    12721 tgaatgccgt tgcaggcggt tctagtgata cattcaaatc cgttgccatg atgctaacgc
    12781 agacggcagg agctgggaaa ctaacaactg aaaactggaa tcaattagca gatgcgatac
    12841 cgggtgcttc aggattgtta caagacgcta tgttgaaaaa cggagcttat acaggaaact
    12901 tccgtgatgc aatggcgcaa ggtcaaatca cttccgacga gttcaaccaa gcaattgtac
    12961 agttaggtat gaatgacgga gcagttaagg cagccacttc tacagacaca ttgagcggtt
    13021 cttgggagca gatgaaatcc actgtaataa atgggctaca aagtattata gaaaaaatag
    13081 gcgttgaaaa tatcactggt ttcatcaaca gagtaacaaa agggattgaa aattctattc
    13141 ctaaaattac tcaatttata ggttggttga gagatattgg aacgtggatc gttgaaaata
    13201 gagagccact aacatggatt gtcggaatca taggcggaat tacattagca gtaaaagcat
    13261 tgaacgtagc aagtatgttg ctggcaatta ctggcggaac attggcagcc ccttttgtgg
    13321 cgattggtgt agcattaggc gcactagcag gtgctttggt gatagcttat acaaaatctg
    13381 aaacatttag aaatatagtc aatgcggctt ttacagctgt gaaaaacgta gttatgagcg
    13441 ttatcaataa tttggtggaa tactacaaaa tgttgtggag cgtgttgcag tggctttggg
    13501 aaaaaataaa agaatgggct tcatggattg gcaataaatt cattgaaatg aagaacagcg
    13561 ttgtgaatac ggttcagaac ctatggaatg gcgtgaaaaa cttcttcagc aatggcgttg
    13621 gagatacgtg gaataaggta gtcggttggg taaaaaacat tttcaacaaa gcaactgaat
    13681 tgaagaacaa cgtttctgat gtaatcggta acctgtggaa tggtatcaaa gacacattcc
    13741 gtagaggtat cgatacggta ttcaattggt tttcagaact accaacgaag atgaagaatg
    13801 ccattattgg cggtaaaaac gccattgttg atgcgttcaa aagtattttc aacgcagcac
    13861 ttaaagcgat aggtaaacca gttaacgcaa tcatccatgg agcttcatgg gtactagaaa
    13921 aattgggcgc agaacctcta caagaatggg atgtaccaca atacgctaca ggtacaccag
    13981 caggtgggca cccaatcaat ggtccaatga tggttaatga tggacgtgga gcagaaacag
    14041 ttatcacacc agatggtaga gccttcattc ctaaaggacg caatgttgtt ctaaacgcac
    14101 caaaaggaac gcatgtcttg acagcagaag agaccgcgca acttcaaggt tctaaagctc
    14161 ctaagtatcg ttacaaaaaa ggtactaact tctttggtaa catgtgggat agtgtgaaaa
    14221 aggttgctgg taatgtaggt cacacaatta aaaacgtagt aggtgacgtg tgggacttta
    14281 tttcagaccc gggagcgtta gctagaaaag tacttggggg gttagatgta ttaggtggat
    14341 tgacaaagta tccattagaa gtaggtaaag gcatcctatc taaagcaaca agtgcactga
    14401 ctgaaaaaat cactgggttg ttctcatctg gtaacttaga tacctccgta ggaacaaatg
    14461 gcgtctataa atatttggct gatgttgcta agtctgtgat gaagaaattc ccaggatttg
    14521 tggcaactag tgggtataga cctatagacc aggtgaccct tattcacatg gtaaacgtaa
    14581 tgccattgat attgcattac caggcgtcac aggaggctca cctagataca cagaagcagc
    14641 aaactacgct tttgacaaat tcgcttccaa aattggttac gtaatcacta atgggaaagt
    14701 tcgtgaccgt tcaggacaat caggtcaacc agcaactggt gcatgggagc catggcccga
    14761 tggagatcac tatgatcatg tgcatttaaa cggtgtgaaa gacccacaaa acactcaaat
    14821 ttcaggagat agcgtgggag gcagtggggt agaaagatgg cgcaatgtag caattagagc
    14881 gttgaaaatg accggtcaat acagtactgc aaacttaaat gcattactaa atcaaatgcg
    14941 tacagagtca aatggtaatc ctaatgcagt taacaattgg gatattaacg ccaaaaatgg
    15001 aacaccatca aaagggttgc tccaagtgat tgacccaaca ttcagacagt atgcaatgcc
    15061 aggattcaac agcaatattt ttgacccact atctaacatc ttagcttcaa tcagatacgc
    15121 actatcaaga tatggctcac taacaaatgc ctatcgtgga gttggttacg caaacggtgg
    15181 aattgtaaac caacatcaaa ttgcggaaat cgcagaagga aacaagccag aaattattat
    15241 tccgttagat aaggctaaac gatcaagagc gatgcaatta ttgtctatcg caatggacaa
    15301 actaggagtt tctcctaaaa cttatggaaa tgcgacaact gtttccagtg attacgaaat
    15361 cctagaagcc attgaaaaac aggcggcaac aacgaatcag ctgttatcgt tattgctggc
    15421 atttttcaaa ggaaatagcc gaactgatag agatttagct ttagatattc agaagatctt
    15481 ggttaggagg atgtagatgc gaactgtatt actgagaaat agaataaacg aagaaattga
    15541 tttgtccaca gaagactatt ttgctactgg gttgagtaat atggggtttg aagtaaaaaa
    15601 agaacatgtg ggacagtggg ggaatttcag agaaagcagt gagagcgttg aaatatctga
    15661 atttcagtca tctgtaatca tttctgtgca tgggtttcga gaaaaagaac tgtacaattc
    15721 acttgtgcag ttcctatcgg aaggtccgtt tgaactggaa tttgcttttg acggcgaaac
    15781 aatggtaaga agatgcagtc taaaatcttt aagtaagact gaaatcgatc cgaaaacatc
    15841 gttactaaca gacactttag aactatattt cacatcaaac tggtattccg taaaacgcga
    15901 aaaattaatt cagcgaccga atgtagtgaa aacacgtggt aaggtttttc cgtataaaag
    15961 atcttatatc tacactcaaa acttgtggga gaaaaaaggt gtattcaaat tcaataataa
    16021 ttctgtatac ctaacgaata gcaaggaacg gatgtccccg ctaaaaattc gtgtgattgg
    16081 gaagtgttca aatccgtatt gggaagtaat ccaaaactca caaatcatcg caacggatgg
    16141 atacttcata gatatgactg aaacacagac tctagaagta tcaagccttt ttgaagatac
    16201 aacggcgatt ttaaaagata ttgcaggggt agaatcttct gtctaccaac aacaggatta
    16261 tacaaaaact aattttgttc aggccccaac tggagaattc agcattgtgt ttcatgttgg
    16321 gggggcagac gtagagattg agttatacga ggagcgtgat ctgttttgat tttagcagtc
    16381 acgctttttc atcgtgattt aatgctttat aacgaacatt tattctctaa cagttttgaa
    16441 tttggtgtgg acgagatcaa cgaagaggct agtagcttta cgatggataa gtacgttcct
    16501 gtaaaaacag gcgattttct actagcaaaa tatattccta gtggaaaatt tgcatatttt
    16561 ggtgtaatta cgtcgcagga agacgaaaaa atcagttgta aaagcttact tagtttagct
    16621 gatagtgaga taccgactgc acgtgtgtca ggagataact acgaagagca tatccggcgg
    16681 ttgattgaat actatttgct gaatgatcca acgaaacaac taaaagatat tttagacgtc
    16741 aaagcagaaa gtgcgacctc tcattcgtat caagctaccg atacgaataa acacaagtta
    16801 agtgcgtata ttctcaatgg tttcaaaaaa tacaacgtaa aatggtattt caagggaatt
    16861 caaaacagaa aaatttatac aggcatacgt gctgtaaatg aatcaattta cattaaagac
    16921 aattcttctg aatttagcga ttgggatgtg tttgttcagg cgccgggcgc tggaaacgag
    16981 aacaagctat taatcgttga taaagcaatg aaagatatag agaaaccgat aatactgtca
    17041 acatggtatt tggacgaaga aaacaatttg acacaagatg gatcgaaaga aaatatcacg
    17101 aaaccaactg tcaatttggt taatatctac gatcaaaccg cagaagataa agcatcctac
    17161 gaagatgtgg caaaatcaga gttgaaaggc aatacgtatt cgcatgaaat caaagtgaac
    17221 gttgtaagaa atgcaaaaaa tttgaatgtc gaaacgattg aaacagggat gtttgccacg
    17281 atttcttata aaggaaagat atacaagtcg gttttgacag cttggcgaat atcaagtgat
    17341 aaggagtttg tggaattgac tttcggaaac ataagaagtc gttttatgga ttattttgaa
    17401 gataatgggg gataaaaaat ggttagcaat gtggatggat atcaatttga aaacgtgaaa
    17461 gtaagcgcag aaaatgatgc tagactttat cacgttttat ataatcggaa aaatcaggtt
    17521 attgatggtt acgatcagtc tatgaattta tcttcaagcg gattaacagt aaaagttgct
    17581 gcaggagcag cgattattca aggtcgtatg gtcgttgttc gacaagaaga aagtataaca
    17641 gttccagcaa actcaagtgg ttatatagca ttaacagtgg atttgacaca agaagttata
    17701 cctggatcta ttcttccaga atcggaagaa tatgaatgga ctaataatca agtcaagcta
    17761 gaatttataa caaaagttat aaaaggtaat ttaaacaatg gtgataaggt ctataattta
    17821 ccactatgct cagttaattc tactggatca actgtttcaa tctcaaagat atcggatagt
    17881 tacgagctga ctctctctaa aggagaaatt ttgtggaggg ggactgcgtt aatgcatgat
    17941 actcaaactg tccaaccttc aaaaaaaatt tggcagacag tttcaggttt tttgttagta
    18001 tggctcccat acgaaaacgg gcaagcaatt gaggatagat atgtaactac gcctttttat
    18061 aaggagcgtg taacttttac taatgtttta ggagaaattg tttcaggatt tgatgactat
    18121 cacaaaaagt ggtttagtaa acgaataaac tataattcga atactaatat atttacaggt
    18181 gcagcaagca atgcaagtgg agataacgca aatatggtgc ttaggtatat agtttctttt
    18241 tagttaggag gtatggaaga gtggcaaatt tagaaattaa attatctgca aataagagac
    18301 agccttatct acgtcaccgt gttgttggta gagttggtga tggggggctc acaacaatca
    18361 atgtacaact tcttgaggaa gatgaaatta caccttttgt aattaatcta aacggtactt
    18421 tgaaatttgt gggcgaagtt tcaaacggta actataccga gggagaacca gaaataatcg
    18481 attcgactaa tgggttaatt agttacacgt tcactaagtc aaatttcagc acgagtcatc
    18541 aattcaaaca agcatatttt gaatatgtag atcctaacgg caaaaaagta acttttcaga
    18601 acttcatcat agacgtgtta gaacgagtgg atattaattc ggagcaagcg aaatactata
    18661 tttcttcatt ggaaaaatta cagagtgaaa tgcaaaccac tttcaatcag ttcattagtg
    18721 ataaacaggt ccaatacgat caaatctact cgaaatataa cgaattagta agattgataa
    18781 atgaatcaga taagcaagta aatgatcgta ttgatcaaac caatcagcaa atcggcgatc
    18841 tcggcaagct gaaaaagatg tacagtaaca gcatcgactt tgggggctat gattattcgg
    18901 ggaatccgaa tttgttaagt aagctatcat acgacttaat tgaaaatcaa aatacttcag
    18961 ctggaacact ttctaagggt gaaaactcgt ttaaatataa taagatatca gctgaagtgg
    19021 aaggtggagt agagttatat tataaacgaa gaggtatagc taactggtta ccctctaata
    19081 aaacgcttgt aatgactgtt aagcttagag ctggagcgga ctatagtcca gttgacggaa
    19141 aacttatact gattagatat aggtatgttg acagtggaac tggcaagatt gttttagact
    19201 tacctattaa cagtaattcg ataactcagg aatggaaaga gtttagtatt actggaacta
    19261 ctccaacatt tagcccacaa gcataccatc cttggataca atttagggct caagatgggg
    19321 tacttgggga aatagaaatg agctatgaca tcaaaatcga agaaggctct acagctacac
    19381 catatcagcc taacttactt gatgactctt actggctagg taaaacaccg ttgggtgaga
    19441 atttgataac gaatgccaag ttcccaatta taacacgaag caatcctatt tctggttttg
    19501 atatttcaga agaattaata ataggtgaaa cttatacggt atctttaaaa gggactaagc
    19561 cagctactaa agaattccac ttatactacg gagaagcaaa ttaccagcag ttgcagtatc
    19621 aggcaacttt acttcctgtg gaaggattaa cagatgtgtg gagtgccact tttacagcca
    19681 gaaatactag tgaaacgaca aatttactta gagtagcgtt atggcaaaaa cctaattctg
    19741 caacatatga tactgttcaa attgactggc tcaagattga aaaaggcgac acacgcacac
    19801 caaatatcag tcagtttaaa tactttggtg aaggcttgaa agacagtaac aatccgaacg
    19861 actacagctg ggatgtcaca ccagagtata ctgaaaaaag cttgaataat acggttagtc
    19921 tgactgaacc gcaatccatc gaaggattga aaaatttcga agatggtatt caatcaaaag
    19981 gaaaatctgt attgacatca gacgacaaca aatatgaagt cgtaacctta acagttacaa
    20041 acgggaatac cggatcagca aagctttatc gtgaaggaaa aaccgtcact atttattttt
    20101 ttgcgttgaa tgggaaaagc agtggtggaa atgattcaac gatactaaca attccagaag
    20161 gctatcggcc accaattagt tttgagcaac tggttggctc gatagaccgt tctactttga
    20221 acagtgctca gttatctatt ggtgcagatg gagccattaa atggcgaaga aactcaagtt
    20281 atggatcgga ttataccttt gcaattactt acacgattta gaaaggcgtg aatcgatatg
    20341 aaggcagcat atagaccaat tgaaccttac ggattcgagc aaatcattgt gaatgatgaa
    20401 gaacatttac cggaagaatg cacagaagtc gaaccaccga ttccaaattg gaaaccgaaa
    20461 ttcaattact gggagggaaa taaatgaaaa acatttggaa atatggccgt actggcggag
    20521 agtatgcagg aaaagtattg gacgacatgc ttgtatccgt tccttacaca gatcagccac
    20581 cgctcgaagg ggttcgttca gatggcgaac cgctaacgat tgctgatcag atgtttgatc
    20641 ctaaattgaa ccaatggatt attttagcga acgcgttaga tcacaacgat ttaaacaatc
    20701 tcaaagcgat gtacgaggct cttgaacatg aaaacggcaa cctaaaacag ctaaatgcca
    20761 aactcatgct aagcgatgta gcgattaaac aggaaaatac tgcattgaaa gaaaaagctg
    20821 acagtttagc acaaatcaat tcaaaaatga tgcttgcttc gttacaaaat agcaaagaca
    20881 tttcagaaat taaagagcaa ctaaatccag cttcaaaggg aggtgagtag tatgtttagt
    20941 tttagcgatg tgaaaatgat gtatgattgg ggctgtttta ctgacgatca agttcgtcta
    21001 ttcgttccac tatgtatcac agatgaagaa gcagatagaa ttattagcaa agaagagagc
    21061 gcatcttaag tgatgcgttt ttttgttgga aagttggtgg aacatgaaag aagaagcgct
    21121 ccaagacgtt gtggagagat tagtaagaat tgaaacaaaa ttagacaact acgaatcact
    21181 tagagaaaaa gctgatagtg caaaagattt ggcagacaaa gcctactcag tagcgctaaa
    21241 caacgcagaa gatatcaaag aaatgaaagc caataataaa tggtcgtggg gttacatgat
    21301 cggtttaggc attacgatca ttggctattt cttgactaaa ttgtaaagga gttaagaaga
    21361 aatgatttta cccgataagt attatcaagt cattaaatgg acggttttaa cagtattacc
    21421 agctgcatct gttttagtag ccacgttagg aaaagcgtat ggatggaatg gaacagatat
    21481 gacagtactc actatcaatg cagtagcaac atttttaggc gttatcactg gtgtgtctgc
    21541 atataactta aaaaaatagg aggaaaaaaa tgaagaagaa aatcatttta tcattgagcc
    21601 taataatggc tcttttttta ttgccaatta acgggttcgc ctatacgatc aacaatgaat
    21661 ttaatttagg tgtaaatgaa ggtagctc
//
LOCUS       DAHHZJ010000036        19976 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.037, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000036 DAHHZJ010000000
VERSION     DAHHZJ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.037"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            27..3416
                     /locus_tag="NBL02_001076"
     CDS             27..3416
                     /locus_tag="NBL02_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCD2571779.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            3419..4840
                     /locus_tag="NBL02_001077"
     CDS             3419..4840
                     /locus_tag="NBL02_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCD2571780.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            4837..6714
                     /locus_tag="NBL02_001078"
     CDS             4837..6714
                     /locus_tag="NBL02_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCD2571781.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            6806..7648
                     /locus_tag="NBL02_001079"
     CDS             6806..7648
                     /locus_tag="NBL02_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCD2571782.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(7800..8675)
                     /locus_tag="NBL02_001080"
     CDS             complement(7800..8675)
                     /locus_tag="NBL02_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCD2571783.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            8882..9913
                     /locus_tag="NBL02_001081"
     CDS             8882..9913
                     /locus_tag="NBL02_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCD2571784.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            10002..10307
                     /locus_tag="NBL02_001082"
     CDS             10002..10307
                     /locus_tag="NBL02_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HCD2571785.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            10486..12501
                     /locus_tag="NBL02_001083"
     CDS             10486..12501
                     /locus_tag="NBL02_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCD2571786.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            12503..12787
                     /locus_tag="NBL02_001084"
     CDS             12503..12787
                     /locus_tag="NBL02_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCD2571787.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            12813..14186
                     /locus_tag="NBL02_001085"
     CDS             12813..14186
                     /locus_tag="NBL02_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCD2571788.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(14269..16284)
                     /locus_tag="NBL02_001086"
     CDS             complement(14269..16284)
                     /locus_tag="NBL02_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HCD2571789.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16306..17520)
                     /locus_tag="NBL02_001087"
     CDS             complement(16306..17520)
                     /locus_tag="NBL02_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD2571790.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            17741..18685
                     /locus_tag="NBL02_001088"
     CDS             17741..18685
                     /locus_tag="NBL02_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD2571791.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            18981..19925
                     /locus_tag="NBL02_001089"
     CDS             18981..19925
                     /locus_tag="NBL02_001089"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCD2571792.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT         6811 a         3680 c         3823 g         5662 t
ORIGIN      
        1 agaaaaaaag aaaaaggagg ggaaaaatga cgaccacagg gaagaaactg aaagttattt
       61 tcatgctgat aatattgagt ttatcaaact ttgtgccatt atctgcaata gcagacacta
      121 cagatgatcc aacagtttta gaaacaattt cagctgaagt catttcggat cagtctggaa
      181 aaaaagcact gaacatcaag ctaaatgcga ataacaccag tgctgaaaag atagaaaaag
      241 aaattggtct agtcgaaaat tacttaagtg atgtggaaag aaaagaagga gatggctatg
      301 cttatcaggt aaatagcggg aaaattacgt tggaaatctc atcaaacact aaacaaacta
      361 tcgatctgag ttttccaatc gatccagcac tttaccacag ccaggcaaac aagctgatcg
      421 tcgataataa agaatatgac attattgatg agacagaaaa taagaaagat acagatgtgt
      481 cagtaccaaa gccagacgaa atagaagaag aatcatcaaa agaaaacgaa aattctgtca
      541 gcccatttac attgcctaca ttatccttgc cagctgtgag tgtgccatct aatcaaacga
      601 ttcctacaga atatacaaca gatgatcagg gcacttatcc taaagccagt tggcaaccta
      661 caggaaatac aaatgttctt gatcatcaag gcaataaaaa cggaacaaat caatgggatg
      721 gtataaattc ttggaatgga gatcctaatg atcggaccca ttcgtatatc gaatatggag
      781 gaaccggtaa tcaagcagac tatgcgatac gaaagtatgc aaaggaaaca agtacacccg
      841 gattgtttga tgtttatttg aatgctcgtg gaaatgtaca aaaagatatc acgcctcttg
      901 atctcgtatt ggtcgtagac tggtcaggaa gtatgaacga caataatcgg atcggtgaag
      961 taaagattgg tgtcgatcgt tttgtcgata ctttagcaga tagcggtatc acagacaaaa
     1021 tcaatatggg atatgtcggc tactcaagcg aaggatatag ctacagtaac ggtgcagtac
     1081 agatgggttc atttgattca gtgaaaaatc aagtaaaatc cattacacct tcacggacaa
     1141 atggtggtac ttttacacaa aaagcactaa gagatgcagg aagcatgcta tccgttccaa
     1201 atggacataa aaaagtgatc gttttgctga cggatggtgt accaacattt tcctataaag
     1261 tacagcgggt acacgcacaa tcaagcagca attattacgg aactcagttt tctaatacgc
     1321 aagatcggcc gggaaatact tctctaatct caagaatcta tgatgcacct gaccaaaaca
     1381 atctatccag aagaatcgac agtacgttta tcgcaaccat cggagaagcg atggcactca
     1441 aagaacgagg aatcgaaata catggtcttg gcatccaact tcaaagcgat ccggcagctg
     1501 gtctctcaaa agcagaagta gagtctcgta tgcgacaaat ggtttcatca gatgaaaaag
     1561 gcgatcttta ctatgaatca gctgatcatg caacagatat ctctgaatac ctagccaaaa
     1621 aagctgtaca gatctcagca actgtaagca atggacaaat aaatgatcca atcgcagaac
     1681 cattcattta tcagcctggt acactttcag tcaagagtgt ggggacaagt cctacaacgg
     1741 tcactccatc tatttccata gaaggaaata ccatcaagag caatcagatc tatttaggaa
     1801 aagaccaaga aatccaaatc cattaccaag tgagaatcca aacagaaaat gaggacttcc
     1861 atccaaattt ctggtatcaa atgaacggca ggacaacttt ccagccaaac attgatacca
     1921 atgaattagc tgaattcggt ataccatctg ctaaagctcc cggagtcagt cttcacatca
     1981 aaaagttatg ggaagaattt gacaacaatc tagctgatcg tccagatcaa gttacttttg
     2041 agattcaacg ggaacatacg acaaatgctg cagcttggaa aaacggatat attcgaatca
     2101 ttaaaccagc taaagataca acaaatacgt gggaacgtgc agacattgac aaattatctg
     2161 caaatagcgg agaaagttat caagagatat tatcactacc tcaatacaat aatcaaggtc
     2221 aagcattcag ttaccaaaca atcaaagaat tacctgtacc aggatacgat tctcaacaaa
     2281 tagatgcaat gacatggaaa aatactaaac aattcacacc gttaaacttg aaaataacga
     2341 aaaattcctc tacaggtgaa aaggatctta ttggcgctgt tttcaaatta acaggagatt
     2401 ctattgatac tttactaaca gatcatggcg acggaaccta ttctcttcca gaaaatgtca
     2461 aattgcaaaa agaaatgacc tatacgctga cagaaacaaa agctccagaa gggcatggat
     2521 taagcaaaaa gactacttgg gaaatcaaga tcgcttctga tggtacggta accattgatg
     2581 gaaaaacagt cactacttcc gatgatacga tccagttgac tattgaaaat ccttttgttg
     2641 aagttcctgt agcagtacgt aagtatgcga tgcaagggac ggacaaagag ataaatctta
     2701 aaggagcagc attttcccta cagaaaaaag aagcaaatgg tacttatcag ccaattgaca
     2761 gccaaacaac gaatgaaaaa ggtcttgcca gttttgattc actcacacct ggtaaatatc
     2821 gagtcgttga aacagctggt cctgccggat atgatacttc gccgggaaat tatgaattcc
     2881 aaatcgataa atatggaaaa atcatttaca cgggaaaaaa taccgagatg acaaataatg
     2941 tatggacgct cactcatcaa aatcgactaa aagcgtttga tctaacggta cacaaaaaag
     3001 aagacaacgg acagacatta aaaggagcaa aattcagact gcagggacca gaaatggact
     3061 tagaatcgcc aaaagatgga caagaaacag atacctttct attcgaaaat ttaaaacctg
     3121 gaacttatac gctgaccgaa acttttacac cagaaggata ccaaggtcta aaagagccag
     3181 ttactatagt tatacacgaa gatgggtcaa ttcaagtgga tggacaagat catgaatctg
     3241 ttctgtcacc aggagccaaa aacaaccaga tttctttaga catcacgaat caggcaaaag
     3301 taccattacc tgaaacggga ggaattggcc gtttaggaat ctatctagta gggatgattg
     3361 gttgtgcgtt ttctatttgg tatctttttt tgaaaaaaga aagagggggc agctaaatat
     3421 gaaaaaactt ggttggctta gtatgtgtct cttcttgtta ctatttaaac cagcttttac
     3481 tcaggtagca acagaaacag aaacagaaat ggttcagatt actttacaca aattgctttt
     3541 cccaaacggg caactgccga aaaatcatcc aaatgacgga caagaaaaag ctttattaca
     3601 aacgtatcga ggattaaatg gtgtcacatt ccaagtttat gatgtcacag attcttttta
     3661 ccatctacgg gaaaagggca aaacggtaga agaagcacaa gcagagatcg caaaaaacgg
     3721 tgcgtcttcc ggtatgttta ccgcagaagc aacaactaca actcttaaca acgaagatgg
     3781 tatcgcttct ttttctctgg ccgctaaaga tcaagaaaaa agagataaag cgtatctttt
     3841 cattgaatcc aaagtaccag aagtcgtcaa agaaaaggca gagaatatgg tagttgttct
     3901 tcctgtacat ggacaaaaca atcaaaaact ttcaactatc catttgtatc ctaaaaatga
     3961 agaaaacgac taccctgatc caccttttga gaaggtatta gaagagccta gaaatgattt
     4021 tacgattggt gaaaaaatca cttattcctt gcatacgaca attcctgtaa atatccttga
     4081 ctatcaaaag ttcgaattgt cagatagtgc ggatgaagca ttaacgtttt tacctaatag
     4141 tttaacgatt tcatcgaatg gagaaaagct gacagaaggc tttgtcatac acaagaaacc
     4201 tcacggattt gatgttttat tttcgatccc ttcgttggaa aaatatgctg gaaaaaaact
     4261 gaccatttct tatcagatgc agctaagcag tacagcacag gcgaacaagg aaatcaacaa
     4321 caacggaaca ctggattttg gttttggtgt cagtacaaag aaagtctctg tatatacagg
     4381 gagtaagcaa tttgtcaaaa tcgagacaaa taaaccagat aaacgattag ctggcgcagt
     4441 attccttatt aaaaacaaag caggaaatta cctccagcaa acagccaacg gatacaagtg
     4501 gacaaagaac gaatcagatg cgcttcacct gatttccgat aaaaatggcg ctttttcaat
     4561 ttccgggttg aaaacaggaa gttatcgatt aaaagagatc gaagcacctt ctggttatat
     4621 tttaagtgaa acagaaattc cgtttaccat ttcaactttt ctttctgagg ataaagaggc
     4681 ggacagtata ttgaaagtag tcaataaaaa agaaaatagc cgtccatttc ttccaaaaac
     4741 aaacgaaacg aaaaatacac ttttaggcgt tgttggtatg gtattcgcaa gctttgcaat
     4801 ctggttgttt atcaaaaaaa gaacaggagt gaaaaaatga aaaatcataa aaaaataaac
     4861 gttatgttag gagtcctttt ccttatttta ccattactca caaacagctt cggcgcaaaa
     4921 aaagtgtttg cagaggagac agcagctcaa gtcatccttc ataaaaagaa aatgactgat
     4981 ttacccgatc ctttaatcca aaacagcggg aaagaaatga gcgaattcga tcaataccaa
     5041 ggattagccg atatttcatt ttcagtttat aacgtcactc aagaatttta tgcgcaacga
     5101 gataaaggag cgtccgtgga tgcagcaaaa caagcagtcc agtctttgac tcctggtaca
     5161 ccagttgctt caggaacgac agatgctgat ggaaatgtca ctttatcttt acctaaaaaa
     5221 caaaatggga aagatgcagt ctacacgatc aaagaagaac caaaagacgg agtgtcagct
     5281 gccgcaaaca tggttttagc tttccctgta tatgagatga tcaaacaagc agatggctct
     5341 tataaatacg ggacagaaga actagatact atccatctct accctaaaaa tacagtcggt
     5401 aatgatggaa cgttgaaagt tacaaaaatc ggtactgccg aaaacgaagc actaaatgga
     5461 gcagaattta ttatttctaa agaagaagga acaccaagcg tcaaaaaata catccaaagt
     5521 gtcacagatg gattgtacac ttggacaact gatcaaacca aagccaaaca tttcattact
     5581 ggtcattctt atgacatcgg caacaatgac tttgccgagg catctattga aaaaggccag
     5641 ttgatcgtta atcatttaga agttggaaaa tataatttag aagaagtaaa agctcctgat
     5701 aatgcggaaa tgattgaaaa gcaaacaatc acgccttttg agatcctggc aaatagccaa
     5761 acaccagtag aaaagaccat caaaaatgat acgtctaaag ttgataaaac aacacctcaa
     5821 ttgaatggaa aagatgtcgc aatcggtgaa aaaattcaat atgagatttc tgtcaatatc
     5881 ccattaggta tcgctgataa agaaggaacg caaaacaagt acacaacatt caaacttatc
     5941 gatactcatg acgctgcttt aacatttgat aatgattctt caggaacgta tgcttatgcc
     6001 ttatatgatg gaaataaaga aatcgaccca gtaaattatt ctgtcactga gcaaacagac
     6061 ggattcacgg tttcagttga tccgaattat attccttcat taactcctgg cggtacattg
     6121 aaattcgttt actatatgca tttgaacgaa aaagcagatc caaccaaagg attttctaac
     6181 caagcaaatg tcgataacgg gcatacaaat gatcaaacac caccgtcagt cgatgtcgtt
     6241 actgggggca aacgatttgt taaagtagat ggtgacgtta catcagacca aacacttgct
     6301 ggagcagaat tcgtcgttcg tgatcaagat agtgacacag cgaaatattt atcgatcgac
     6361 ccatccacaa aagccgtcag ctgggtatcg gcgaaagaat cagcaacggt ttttacaacc
     6421 acaagtaacg gtttaatcga tgtgacaggt ctaaaatatg gcacgtacta tctggaagaa
     6481 acgaaagcgc cagaaaaata tgttccatta acaaaccgtg tagcatttac tatcgatgaa
     6541 caatcttatg taacagcagg acagttgatt tctcctgaaa aaataccaaa taaacacaaa
     6601 ggtacacttc cttcaacagg cggtaaggga atctatgtgt atatcggtgc aggagtagtc
     6661 cttctactga ttgctggact gtactttgct agacgcaagc acagtcagat ttagtctttt
     6721 tgcaagaatt ggaaaaagga gcagcggatt acgcaaatgc tcctttttct tttattatca
     6781 aacagtcatc acaaggagaa cacaaatgaa accaaagaaa aggaaacttc tagatctgct
     6841 gatgcttttc ttgcttctct caggaatagg gatactagct tatccatttg ttagcgacgc
     6901 gctgaataat tatctggatc agcagatcat ctctcattat cagcaacaag cggtcaaaga
     6961 aaatgaagaa gtcatggcta aaatccaaga aaacatgaca aagaaaaacc agcaattagc
     7021 aaaagaaggt ggaaatccag gtgcagatcc ctttactaag aaaaaagaac ctgcgaaaaa
     7081 ggatagaacc tattttgaga aacatacgat cggtattttg acgatccctg agatcaatgt
     7141 ccatttgcca atttttgatg aaacgaatac tgtgctgcta gaaaaaggtg cctcattatt
     7201 ggaaggaacg tcttatccta cggggggaga aaatacacat gccgttattt ccagccatcg
     7261 aggactgcct caagcaaagt tgttcacaga cttaccgaaa ctgactttag gtgattcttt
     7321 ctatatcgaa atcaatggac ggacactcgc ttatcaagtc gatcagatca agacggtaga
     7381 accgactgat acaaaagatc tgagaatcga aaaagggcag gatttcgtga cactcctaac
     7441 ttgtacacct tatatggtca acagtcatcg cttacttgtc agagggcacc gggttcctta
     7501 tcatgcaaaa gaagttcaaa aagaagtaca gaaagtatcc gagaacaaac gatttttcct
     7561 gtatggattg ttagctgcat gtatactgat tttcctcctt cttatatatc tttttcgtca
     7621 atcgttcaaa ataagaaaca gaagttagct attcctcatt tctataatat atctattcga
     7681 atcgttccgt aaacaaacag gctgaggttt gaaaaagaaa acttcagcct gtttttatga
     7741 tgatatcaaa gagagcctat caaacactat ctttcttatt actaaatgag gatcttttct
     7801 taagttttcc gatattcttt aagaaggcct ttttgcatca attcttgttt gtttttcaag
     7861 ttgcggatat tctcttcaaa attttgagaa tatatcactg agaataacgt atttaatacg
     7921 taatcgaagg caatttgaga ggagaaagtc gctactttag caaaatcata ttcctcctgc
     7981 gatataacaa gacacatatc agcctgtttg gccatttctg aatgctgatt tccggtaatc
     8041 agcaaggtag gggcctctac atgcttcaaa taggtcatta ttcgttcata atgatggatg
     8101 cgaccgctgt agctaataaa aatggcgcaa tcctcgctag tcaaattggc tgcattccaa
     8161 gaagaatcac tgtattcttc agccatgatc aaaaacttat tcaacttgac cattttattc
     8221 tggaatttcc ttgccgtgat ttgagaatca ccttttgcaa aaagaaagat tcgctgtgcc
     8281 ttgttcaata ctttaccgat ttgatctaaa tcttcatgtt caatctgcaa ttgtgctttt
     8341 tttattgact ggatcgataa atccgccatt ttctttgcaa tttgctgagt gctatcttca
     8401 cttgaaaaag gaaaattagg atcaatcgtt tgattgaact gagcaatcaa ttgatgaact
     8461 tcccgcccca gctcgaaacg gaattcttta aatccgctga atcccatttt tttgcataaa
     8521 cgaatgatag ctgaatggga agtatacgta gcttttgcta attcttcaat cgtcatgtag
     8581 actgcagctt cgatattcct acggatgtaa tcgacaatcc gtttctctgt cgtggtaaac
     8641 aactcttgtt ttgttagttt ttcttcaata agcatgatct gacctccaaa ttctatctta
     8701 ctgataaaca cttggttccg ctactatctt tttgattttt atccttactt tgaacattaa
     8761 tttcaccaaa ccgaatgata acaagtgttt ttctgaactt ttttaccaga atagctatta
     8821 taaatacttt ctgcgctttt ctaggatata ctgaaaacat caataaaagg aggcaccact
     8881 catgcttaca atcgcttatc ttggcaatgg aaagagtaca aatcgctatc atttgccttt
     8941 ttcaagaaaa cttacgaaca aaatcaatat caaaaaaatt ttttccccat ctgaaccagt
     9001 gtggaaaaca ttcgaagaca tcgaatatac tgatcagctg tctgatcttt gggatgatcc
     9061 tgagatccaa ttagttgtca tcactacacc tagtcaattt cattatgaat atggaaaaat
     9121 ggcactcgaa catggtaaga atgtactagt agagaaacct tttgcagaaa cgtcaaagga
     9181 agcagaagag ttatttgcat tagcaaaaga aaaaggtcta tttatccaat gttaccaaaa
     9241 cagacggttc gattctgact tcttgacagt ccaaaaagta atcgaaagcg gaaaattagg
     9301 ggatatttta gaagtagaaa tgcattatga ttatttcaga ccggaagtac cagaaaatac
     9361 gcatgaattt tctactgcga acagctattt atatggacat gcttgtcata cagtagatca
     9421 agttctttct tatttcggcg aaccagatga tatcagctat gatgtacgtc aacttttagg
     9481 gaaaggacga atgaatgatt acttcgatct ggattttttc tatggaaata tgaaaatttc
     9541 gataaaatcc agttatttcc gcatcaaaga acgtccaagt ttcgtcgttt acggaaaaaa
     9601 agggatgttc gtgaaacaaa caaaagatcg acaagaagaa catctgaaaa tgttttacat
     9661 gcctgatcat gaagactttg gtatcgattt gcccgaacat tatggtgtat tgacctatat
     9721 agatgaacaa gggagttatc atgaagaaaa agtaatttcc gaggttggtg attacagccg
     9781 tgtctacgaa gggatttacg agacattagt taatggcgca gaaaaagtca tcaaagatga
     9841 agaaacaatc ttacaaatga aaattttgga agaaggaatc aaaagcattc aagaatcaga
     9901 aggaaaaata tgacatattt gtaagaatcg ggtttgtttt ttgacgcata aactgtctta
     9961 ttgtacgatg aaaaagtata gaactactag gaggaaccaa catgacaaat tatgacggtg
    10021 aaaaacacaa acgattaaga ggttcaatgg cccctaaaac agaagaagaa gtggctttag
    10081 caggaactaa ttctggtgaa acagaagaca aagaagaatc attagctggc acaaactctg
    10141 gtgaaacaga aaataaagaa acagctttat ctggttcaaa ttctgtagaa gaggaaacaa
    10201 agcctaatga gcccaatcac tttgaaaaag atatcgaaaa agcggttgaa gatgtcaaag
    10261 aaaaatttga agaactgacc tcaaaaaata aacatgatca tcattgatcg gacgaaatca
    10321 attcataaaa gcaggagaca tttggtcacc tgcttttttt ctgtatccat tcgatagaaa
    10381 tcgttcggta cagtcgtaaa aaaaatccaa ctgtaagttg taccaaacac ccctcgattt
    10441 aaaagcgcta ccatactaga atataagtag gaggtgaaca gcgctgtgaa ctacaaaatt
    10501 agttatctcc tgaaaacacc aagagaaatc agtctagaaa aagcagcaga gatcatcgaa
    10561 gtaaatacaa gcgaggcgat caaacaatta actccttttg ccgaatatgt ggatgatacc
    10621 cgtatccgta taaagaaatt ccgagcaatc gactgggcag aaaaattttt ggatattact
    10681 cgatttgaag ctgtgtatac cgaacaagag cgaagagatc ttctttattt gatggtttat
    10741 tcgactgcaa cgtctgtttc tgtttttcat tttcaagatt ttcttcaggt ttctaaaggg
    10801 acagtcctgt cagatatcaa aaagataaga aatgaattag aagtggaaga ttgtcagctg
    10861 tcgtatacta ggaaaactgg ttttaggctg ctagggtcag aagcaatatt aagaagagaa
    10921 gcaaaaaatc gcactgctcg tttgatgcag tcacttgctg gaagatttgg gctcacgtat
    10981 ttaaccgcaa ccctttccat tgattattac gccaaagtta gagatttgct agataaaacg
    11041 atttcactat cagatcttcg attcgcacca aatcgaatgg atgaattagc ttattatttg
    11101 agtttgacaa ccattcgaat tggaaaaaca tccatggaac tggtaaaaga tgctgaactg
    11161 ttggcttctc ttgcagtcac tgacattagt cgacaagtat tgactggcct tggctattcc
    11221 gaacatcaaa attcggagat tatatttttc tctctttgct tagcatcgat cgttgaagga
    11281 gatgtccagg agcctgcact tgattttttg ctggaatgtt cttcagaagt catccatcgc
    11341 atggaattcc tgatggccgt agaatttgct tcgtttcgtg aattattgat gaatgtcttt
    11401 tatcacttgg tccctgccta ttttcgtatt tcctattctt tttatttacc taatgtgatg
    11461 atcgatcaaa ttaagcatca atatgcttcc atctacgaaa tgacaagaaa agcattactt
    11521 ccgttagaaa gacgaatagg gaagagcatc cctgaagaag agattggttt tttcactata
    11581 ctatttggcg gagagatcag aaaagcggac gcagaagaaa gaaatagaaa aataagagcc
    11641 gtgatcgtct gtccaagtgg cattagctct tcattgatct taaaaagtga acttcagcaa
    11701 ttattcccga tgatcttttt tacagagact aattcagctg accgcttaca cgaggttgac
    11761 gaaacttctt acgatattat tttttctact gtacctctca cacttactga taagaaaaag
    11821 tctctctatg ttctgcatcc aatcatgact gacttggaaa aaaatcagct gatcaatcaa
    11881 gtacaaagag attggctgat accaggattt tcaatgcctt ctgcccaaga attgttgtcg
    11941 gcgctactgc cttatatcga attaaaagaa ggcgtagacg aagaaaagct ctatcaagta
    12001 ttaaatcgta aaatgaacaa acttttagaa aagaaggagg atcaacgtcc tatgctatct
    12061 gaattattaa cagaagaaat gatccaatta tcggatcaac caaaaaactg gcaagaagca
    12121 atcacatttg ccgcacagcc gctattatca gaaaataaaa tagaagaaag ttatatcaaa
    12181 gctatgatcg agcgcgtgga gcagtatggt gcgtttatcc atattggcga tcacatcgca
    12241 ttacctcatg ctcgtccaga agatggcgta aacgaagtag ggatgtcttt gctgaaactt
    12301 caacaacctg tggatctgct tgataatcca gcccatccca ttagtttgtt catctgttta
    12361 gctgctatcg ataatgaggc acatcttcgg gctttggcaa atttaacaaa gctactttct
    12421 aataaacaaa atcttcaaga tttattacac gcatcaacaa aagaagaaat attagcgatc
    12481 ataagaaaag gagacgaatg atatgaaaat ttttgcagta tgtcaaagtg gattaggaac
    12541 aagttttatg gtagagatga acatcaaaca agcacttgca gatcttggag cagatgtgga
    12601 cgagtttgaa gtaagtcata cggatgttgg aagtgtatct agcgatatgg ctgattattt
    12661 cttcattgag cattctttga aagatacgct aacttctata cctgatgaaa agctcgtacc
    12721 tttgcaatcg atcattgaca gcgaagaagt aaaagaaaaa gtaacgaaga tcttgtcaaa
    12781 agaataaaaa tgattcaagg gaggaactat caatggataa attgttgaat ttgtttgtgt
    12841 caatcgtcag tcagccctcc atcctcgttt ctttgattgc actgcttggt ctcgttcttc
    12901 agaagaagaa attttcagaa gtgatccaag gaactatcaa aacatttgtc ggattcctag
    12961 tactgatcgg cggtgctggt ttaatcagta attcactaac cccttttgct tcgatgttcc
    13021 aaacagctct gcatactcaa ggagtagttc ctagcaatga agcagtagtg gcgattgctt
    13081 tgcaacaata cggtacacct actgctttga tcatgctagt agggatgatc gtgaatattt
    13141 tacttgctag attcacaaga tataaattca tttttcttac aggtcaagca atgctttatg
    13201 tttcttgtct aacagcagtc attttagtaa gcgcggggtt tactgcaaat ttgtggatga
    13261 tcttattagg cggggtcttc gaaggaacac tattaactat tacgcctgct ctttgtcaac
    13321 cgtttatgaa gaacatcact ggaaacaatc aagttgcgtt agggcatacc ggaaatatcg
    13381 gttatgcttc tagtggatgg atcggaaagt tttttggaaa caaagaccgt tctacagagg
    13441 acataaagat tcctaaaagt ttcagctttt tgcgagattc tactgtatcg atcatgctct
    13501 tgatgtcgat cgtatatgtc gcacttgctt tgttagctgg tcctggattt gttgaaagag
    13561 aactaagcaa tggagacaac gctgtaatct ttgcactgat ccaagctgga acattcacag
    13621 ctgggtttgt tatcgtcctt caaggggttc gaatggtgtt ggcagaaatc gttccagcat
    13681 tccaagggat tgctaaaaaa ctcgtacccg attctaaacc agcattagat gttcctatcg
    13741 tttttccata tgcacctaat gctgtattaa ttggcttttt tgttagcttc tttgtaggaa
    13801 cactatccat gtttgctatg ataggcttgc aaactacagt tatcattcct ggggtagtag
    13861 gacatttctt ctgcggagct gcttctggtg tcttcggcaa ctcgactggc ggtagaagag
    13921 gggcggtatt aggttctgct ttcaatagct tattgattag ttggctgcca ttatttatcc
    13981 ttccagtatt aggagatctt aaaatcgctt cttctacatt tgccgatact gattatctag
    14041 taccaggact tgtacttggg aaactaggag aatacggtca aatagccttg acagttggaa
    14101 tcatcgcttt ctttttactt gtcgtattat ctagctttct ttttgccgga aaaagaacaa
    14161 acattgtttc taaagaacag aattaagcaa acttcaaaat aaaaattgct tggacaatat
    14221 cagaagaaga tttaaagtct tctgatatgt cgcaagcagt tttttgtttt atgcatttat
    14281 ttttgaatag cttgttaaga caagtggctg ttttgctaga agaggataac ttttaccatt
    14341 cattttcact gttccgcctg ttctatcaga agtaatcacg atcttatctt ttgttttttc
    14401 aagcgaaatc tttccatctt tgataggtag cgtacaattc agtgtttcaa atgaatctag
    14461 attaggttgt atcataaatt cttcataacc tggctttgtt ggatacaaac cgataaaata
    14521 gcgtcctaaa agatagattg ggcttgctcc ccatgcatgg cataagcttt ttccataagg
    14581 atctccatac atactgtact tttcatttcc tgtttgagta gggtcgtatt cttcccagaa
    14641 agttaccgca tcatgagata acatccctcc ccaataatca agaatcgttt ggtagacgat
    14701 attcgtctgt ccgagtttgc ataaagcatc ttgttcatag aatttgaaat atggagtagt
    14761 aatctgtgtg atagtgtcat tcaataagac attctttaag atcgattgct gttggtcttt
    14821 gttagcaaaa tcgaataaaa cagcgaaaat attcgcatga cgcgtcacat ttcttctccc
    14881 agattcatag caatcgataa aagcgccaag ttcttcatcc caaaagtagt caaataggtt
    14941 ttgtttcaat agttgatatt tttcttgata gagatgagta tgataatttt cgttctctcc
    15001 atatgtttca gctaagatat ctccgcaagt gatcatggaa tgataggctt tcagtaataa
    15061 gacttgttct gcagcaagcg ttccttcttt atccatttca gaccaatcaa taaatatcca
    15121 atcttttgct ctcccataaa taaaccctaa ttcattcgtt tgctccatca agtatctcat
    15181 taatgattgc atcttagggt agacgatgcg taaaaattca tagtctttcg tcatcatata
    15241 gtggttttcg ataccaatca accacaagat cgaatagtca acgatagtat tcatatgctg
    15301 cttgatttca ttttgtcctc ttaatgctaa tatcgttcgt ttattgattt cctcgtcaaa
    15361 aaaaagatat tgattgatca tgaatgattg atacgcatct ccagaccaaa tccagcgatc
    15421 tcgtttgata ccgtcgataa aaaacaaatc actacataac ttgaaggctt cttctgatac
    15481 tgaccagatc tgattcagta aagaagaatc gcttgaaaaa gcagcttgtt tcgaaagaga
    15541 taaaaattca tgaaaaactt gaagttgaac cattccagta gaaacctctg gtataaacaa
    15601 atagcgaaaa gcgcgtttac gagctttcat ttttcttgtc attcctattt gcttatagta
    15661 gcaataatcg atatccatag cttctacatc ggactctcca taacatagag tgatagattc
    15721 gatatggtca tccagagata ttagttctac acagccattc actgctcgcc caaaatcgaa
    15781 taagattcct ccgccaatct ccgtttcagt gactggatgg acgcattctt tttgataata
    15841 gatttgattt ggatcttttg cgatatcgac ataaagcgga ctccagccag ctggatattc
    15901 ttcaataaaa ttacttgctg tccagccaat atctgatttt acttcgtctc cctcgataaa
    15961 catcgctggt agccctgatg tatgtcctac aaagatacga atttttgctt taccagctgg
    16021 acaagtcagc cattttccac atggatgttt ttgtccatta atttctacat acccaactcc
    16081 ttggatcgtc actttaaaca tggaaggttg atcaagaaag taatatctct taaacttgac
    16141 gttccgatgg caatcatcca tgtaccagta cgctggccag tcaaaacctc gttcttcccg
    16201 ttggaaattt tgtagcagtc catggcggat ctcaaagtct ccaggatacc aaagccatct
    16261 atctttatgt ttttcatttg tcattttaca cgacctccat caaatttatt ttgttgtcaa
    16321 ttgttctttt atttcttcat ctactcttat cactgtcttt ctattcgtcc tgtgtactac
    16381 tagaaggcta ataagcgcgc taatcaacag caagatactg ccattgacga aaacagcaag
    16441 tgttttactc ccaagagaag caaaagagag aaagagcaaa ccatatggtg caatgaaagt
    16501 acctaaatta gcgccaacca ataagattga agtattgaaa ctctccgcgt acttgggcgc
    16561 ttggatactt acttggttga ataagtaagg aatgaataac gaaaaagaaa ttcctgttag
    16621 gatcgcacca aacccaatca catacaggtt tatcacagtg gcaatcaata caaagccgat
    16681 tcccatcagt agtaaagaaa gtgtaagcat atagcgatgt gcttttgtct gaagccagcc
    16741 aaaacaaact ccggcagcca tacttgccaa ttgcatgatt gttaagatat tcgctgcatt
    16801 agtttcgttt cctagagaat aagtcgtcag gactgtggct aatttaatcg atacagcatt
    16861 atataccacg atgaccagca agagaaaaat accataacta atcattttag gattcgtttt
    16921 gattacttgt cttgttgggg ctgtgccatg cgattgaagt tcagttgttt caggtgtagg
    16981 aacaaacaaa acaaacaaga caagaatagg taagctgatg gcgtagacta aaaaggtata
    17041 ttgccaaccg aactgtacta gctgtcctgc aacaaatgtc attaaagctg caccaaatcc
    17101 ttgaaatgca ctttgaaaac caatcataga ggctcgttca tctccgtgat aaaaggcagc
    17161 aatcatgctg actgccaaag aattgatcaa tccaaagcca atacctagtc cagctcttga
    17221 agctaagaca atactgtaac tttgtgagaa gagggggagg ataccacaaa tcgatgcaat
    17281 cactaatccg atgatcacag tttgtttttg tccgattttc ctagcaataa aactactaat
    17341 caaaacaaaa atagtgacca taaaagaggg agcagtcgtt aataattcaa cagcagataa
    17401 agaacgcgtg gcataagtag ctgacatact gggaattgca gacgtgactg caccagcaga
    17461 agtcaaaata agagagactg acagcagaga aaatttcatc atctttttat tcataagcat
    17521 gttcctttca tttttgtaag cgcttatcaa aaatatagta tgaagtggtt attctctcaa
    17581 tagcggaatc acaggaagtt catgtcccaa ccatagaaag tctttatgaa accaaaaata
    17641 gctttgcata taatagcagt acagcgtgaa ctaataagcg aaagaaaccg cttacaaaaa
    17701 ggagggagtc aattggaact agctgaatta gacatttttt ttgcgtaacg aagaatcaat
    17761 cgaaactctg cagcgtgaac aaggaatgaa tatcaatgat ttaaacttag cttaccatct
    17821 tcagacaccg ccaaaaatcc accgagggga tctatttcaa cagggaaata ttcatatcag
    17881 taaacaccgt cgatatgcgg aagttcccga gcatacacat gattttgttg aaatcaatta
    17941 tatgtatagc ggtacatgtt tacaaattat taatggagaa aggatagaga tggaagaaag
    18001 tgacgtggtg atcattgata aagaagctac tcaaagaatc gaatacacag ggcaagagga
    18061 tattttaatc aacatcttat taaaagaaga aaccttatct acagagatat tgaatcattt
    18121 ggcaagcaca aataatctac tttcttcttt tttgataaat gcatcaaaag aaaaagggga
    18181 acatacacaa ttcattcatt ttcgcggcgg aaaaacaagt cgtttgacgc atttgattga
    18241 aaatatgctg ttgatttatt ttgatacata cagtcataag atccgttcgt taaatctcta
    18301 tacatcacta ttactgatcg aattgactca tttattagaa caacaaagct tttcatcagc
    18361 agatggatgc gcagaaaatg agatgcttag agtattagat tatatcgata agcattttac
    18421 ttcttgtacg ttaaaagaaa cagcagctca ttttggctac aatccggtct atcttagtaa
    18481 taaattgaaa atgtattttg atgcttcctt tcaagaactg gttctaaaga aaaaattcga
    18541 ctttgcgttg gaactgattc aagaaacgag tttttcgttg gaagaaatcg ctaatcgtat
    18601 cggatataaa caaacgactt ctttattcaa attattcaag aagtattcaa catgtactcc
    18661 caatgaatgg agaaataaac attaagaaga aaatctttca ctaaagcata tgaaggaaaa
    18721 ttgttttagg tgcgattgct gtcttattat atcgttgcag cgaatttttt tggatttatt
    18781 ttcagttcta aacttttggt tgtattatca ggggatatag gtgtaaacat ttgtcattta
    18841 tctgttatgc gtattcagga attcgtttcc atcaaaaatt ataataacaa ctattataat
    18901 taacatgttg ataaaatgaa taaacaagac tatacttttc atgaaagtaa caaatagtta
    18961 tgaaaaggag ctgtttattc atgaaaaaaa caagtcgtaa agtagtaatc gtcggtacag
    19021 gttttgttgg aacaagtatc gcttatgcaa tgatcaacca aggaatctct aatgaattag
    19081 tattgatcga tgtgaatcaa gaaaaagcag aaggggaagc attggacctt ttagatggta
    19141 tggcttgggg cgatgaaaat gtggctgtat ggtcaggtgg ctatgaagaa tgtaaagatg
    19201 ctgatatcgt tgttatcaca gctggtatca accaaaaacc tggtcaatct cgtttagatt
    19261 tagttaaaac aaatgcgtca atcatgcgcc aaatcgtaaa agaaatcatg ggatcaggat
    19321 tcgacggtat tatcgttgtt gcttcaaacc cagtagatat tttgacatat atcgcttgga
    19381 acgaatctgg tctgccaaca tcacgtgtta tcggtactgg aacaacattg gatacaactc
    19441 gtttccgtaa agaaatcgca ttgaaattaa aagttgaccc acgcagcgtc cacggctata
    19501 tcttaggtga acatggtgat tcagaagttg ctgcatggtc acatacaact gtcggtggta
    19561 aaccagtgtt tgaaattgta gaaaaagacc atcgtattgc ccaagatgaa ttagatgtga
    19621 ttgctgataa agtccgtaat gctgcttatg agatcattga tcgtaaaaaa gcaacttact
    19681 atggtattgg tatgagtact gcacgtatcg taaaagctat cttgaataac gaacaagctg
    19741 tattgcctgt atctgcttat ttaactggtg aatacgacga aaaagatatc tttacaggtg
    19801 tgccatcaat cgttgacgaa aatggtgttc gtgaagttgt tgaattatca atcaacgaag
    19861 aagaaaaagc aatgttcagc aaatcaacaa gtgctttaag agaagtattg aacactgtat
    19921 tataagaaac aatgaatatg aactaaaaat cctaaaactg cttaacagct gaaata
//
LOCUS       DAHHZJ010000037        19754 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.038, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000037 DAHHZJ010000000
VERSION     DAHHZJ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.038"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            149..1600
                     /locus_tag="NBL02_001090"
     CDS             149..1600
                     /locus_tag="NBL02_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCD2571793.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1949..2581
                     /locus_tag="NBL02_001091"
     CDS             1949..2581
                     /locus_tag="NBL02_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HCD2571794.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLTGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3169..4608
                     /locus_tag="NBL02_001092"
     CDS             3169..4608
                     /locus_tag="NBL02_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD2571795.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4903..5121
                     /locus_tag="NBL02_001093"
                     /pseudo
     CDS             4903..5121
                     /locus_tag="NBL02_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5370..5585
                     /locus_tag="NBL02_001094"
     CDS             5370..5585
                     /locus_tag="NBL02_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571796.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5802..6058)
                     /locus_tag="NBL02_001095"
                     /pseudo
     CDS             complement(5802..6058)
                     /locus_tag="NBL02_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6245..7156)
                     /locus_tag="NBL02_001096"
     CDS             complement(6245..7156)
                     /locus_tag="NBL02_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCD2571797.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7175..8179)
                     /locus_tag="NBL02_001097"
     CDS             complement(7175..8179)
                     /locus_tag="NBL02_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HCD2571798.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8176..10299)
                     /locus_tag="NBL02_001098"
     CDS             complement(8176..10299)
                     /locus_tag="NBL02_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCD2571799.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10304..12751)
                     /locus_tag="NBL02_001099"
     CDS             complement(10304..12751)
                     /locus_tag="NBL02_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCD2571800.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12738..13127)
                     /locus_tag="NBL02_001100"
     CDS             complement(12738..13127)
                     /locus_tag="NBL02_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCD2571801.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(13187..13690)
                     /locus_tag="NBL02_001101"
     CDS             complement(13187..13690)
                     /locus_tag="NBL02_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HCD2571802.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13703..13927)
                     /locus_tag="NBL02_001102"
     CDS             complement(13703..13927)
                     /locus_tag="NBL02_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571803.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(14031..15941)
                     /gene="ltrA"
                     /locus_tag="NBL02_001103"
     CDS             complement(14031..15941)
                     /gene="ltrA"
                     /locus_tag="NBL02_001103"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCD2571804.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(16687..16824)
                     /locus_tag="NBL02_001104"
     CDS             complement(16687..16824)
                     /locus_tag="NBL02_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HCD2571805.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(16821..18005)
                     /locus_tag="NBL02_001105"
     CDS             complement(16821..18005)
                     /locus_tag="NBL02_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCD2571806.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(18264..18554)
                     /locus_tag="NBL02_001106"
     CDS             complement(18264..18554)
                     /locus_tag="NBL02_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571807.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(18544..19686)
                     /gene="dcm"
                     /locus_tag="NBL02_001107"
                     /pseudo
     CDS             complement(18544..19686)
                     /gene="dcm"
                     /locus_tag="NBL02_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
BASE COUNT         5838 a         4031 c         3502 g         6383 t
ORIGIN      
        1 tttaaaggca gtaacaggat taaacattga aacataatcg ttactaaatg aaatcaagaa
       61 atatactgta ttactcaatt cattgaaaat atttatcata atttttaata gaaaacacta
      121 ttaaagagaa aaaagagagg taaatcaaat gaaaaaatct ttaaaacttg catcagcagt
      181 tatattaatg tcccaactcc ttatagcaac acctttccat gtgttggcaa gcgaaaattc
      241 aacatacaaa attgcaaaag aaagaaccga gacgactgaa atgagtaatt ccgatgtaac
      301 agcagagagt agtgaacttt tgacaacttc atcaagtgaa agtagtacga cttcaaatgc
      361 cgagtcttca acaacttcat caagtgaaag tagtacgact tcaaatgccg agtcttcaac
      421 aacttcatca agtgaaagta gtacgacttc aaatgccgag tcttcaacaa cttcgtcaag
      481 tgaaagtagt acgatttcaa gtaccgaatc ttcaacaact tcaagtagta caagtgaaat
      541 aacttcttcg aaagtgacaa aaccaaacca tatagaatct tcaaaaaagg aagcgccaaa
      601 aacggaagga caaacaaaaa ctttttcgac accacagcaa atagaatcaa cagaatcttt
      661 ccaatcacaa ccaatactat ctggtaatga agaaattcat tttgacaaaa atgaatctgt
      721 agaaaatttt attatgaaaa ttggtgagtc tgcgagaaaa attggtcaga aatatgattt
      781 atatgcttct gttatgattg cacaagcgat cttagaatct gccagtggtc aaagccagtt
      841 agctcaggca ccgaattaca atttatttgg gataaaagga acttataatg gaaactttgt
      901 cattatggta acaaacgaag atttgggtaa tggaacgctt tatacgaccc aatcaaaatt
      961 tagagtgtat gaaaactatg aggaaagctt tgaagattat gccaaattat taactaaggg
     1021 aattagtgga aataaagatt tttatgcagg agcattaaaa gctaatagta aaacatatcg
     1081 agaagcaact aaattcctta caggacggta tgcaacagat acacaatact atttgaagct
     1141 aaatgaatta attaaaacct acgatttaac caactatgat aaagaagtta aaggtccaca
     1201 aatgagtaag actggatacc aagtgcctat tcataattat tcaataacca gtttgtttgg
     1261 aaatcgtgga aatgaatttc accgaggact tgatatggca gctacacagg gtgagccgat
     1321 tcatgcagtt aaagctggta aggttgttaa agcagagttt cactactctt ggggaaatta
     1381 cgtggtgata caacacgaag atggatcaac cgcgttatat gctcaccagc aacaatacct
     1441 agtaaaaact ggagataatg ttttgcaagg acaaataatt ggctatgtag gaagtactgg
     1501 gaatagtact ggcccacacc ttcatttgga aatttgcaaa gatagtagtc tttctcagag
     1561 catgttagtt gatccgaaaa ccttattatt cggagactaa aaaaggtaat caatctgaca
     1621 aagtaacaag aaaatggttg gattcttaga cctttgatca ggatattgag actggaattg
     1681 atatctcgat tacgatgggt ttgacaaata aaacaagtcc attatcgaat atcgagtgta
     1741 ttattgccgt ctttatgctc atattttgag caggtttcac tagatgaaaa caatttatct
     1801 atcttgagga tttcgtgtac gtatcgttca gtcttgtatt gtaaattagt cataaaatca
     1861 gactaagaaa aattggaaat atttttttct gatttattaa aagttaattg aaattatatg
     1921 gtacttcttt cttgggaggg taggtgcagt gaaggaaaga tatggtcacc ttactgttgt
     1981 tgaggagatt actcataatg gatataaaaa atatcgttgc ctatgtgact gtgggaatga
     2041 aacgattgta tatcgatcaa acttactaac aggtagaacc aaaagttgtg gatgtggaaa
     2101 ttataaaaat aggcataaat ataaaaattt gacaaataaa acttttggta agttagttgc
     2161 aatcaagcca actaaactgc gaagtgaagg aactgttgtt tgggaatgtc aatgtctgtg
     2221 tgggagaaaa aagttagtat cggcaaagcg acttctaaaa ggtgatgtaa aaagctgtgg
     2281 atgtttgaaa tcaaaaaagc atcacattga aaataaaaaa ttcggattac ttactgcaaa
     2341 gtatcctttg gacaataact gttcatggaa aacaaaatgg gtttgtcatt gtgagtgtgg
     2401 cagtatgtgt gagataagtt atagtaactt attttttgga catactcaaa gttgtggatg
     2461 tttaaaaagg caagaaacaa ggacatgtgt tgaagggaca gtaatagagt gtctaacgtc
     2521 aaaattaccg aaaaataata caagtggtgt taagggagtt tatcgttcta aagagaaata
     2581 gattgcgtat ataactttaa aaaaaaagag gtactattta ggatcgtatg attctattaa
     2641 gagtgcagct caagctagaa aagatgctga aaataaattg tttcaacctt tgattgacaa
     2701 atatcgaggt aatcattaat tattgtggat aataattaac agtgatatat aaacatctat
     2761 aatatattat tcatactatt gatatttagt ataatcctat ggaattgaat atcatagcaa
     2821 tgtatttgaa aagcaaattg gaaaacaaat aggtgcaaca ccataaatat gaaaaaattg
     2881 ggaaaaagta gaatggaaat ttgcttacgt ctcacgagat agctcacact attaatctga
     2941 agaacaatga agtcgacttt tagaaattgc tagaagaaag taaagcgaat tatcactagt
     3001 tactatagag taagttcaaa taaagcatct ccaacttaca tggttacatg aggatatcaa
     3061 atcgaaatta ctcaagcttc ctatggctag agtcgcgaga agttgttggg cagtagacat
     3121 aggttatatt gttcttagct acacgatcat tagaaaggaa atcctattat gtatagttat
     3181 caagaagatg caaaagtcca aaaaaatcgc tggtggattt tgattgctat atctatgttt
     3241 accttcatgt ccacattgga tggcagtatt gtcaatattg cactacctac catctcagat
     3301 gatatgggca taccaatgaa tcaagcggaa tgggtagtgt ccgtttatct gatgattgtc
     3361 tgttcttgtt tgttgctgtt tggtaaaatt ggcgatagcc gtgggaaaat caaagtattt
     3421 cggattggta cgctgatctt tgtcttggga tcgctgttgt gtgggttcaa tcattcgcta
     3481 ggtattttat tgttcgcacg ggtggtacaa gccattggtg ctagtatgac tatggctaca
     3541 aatgccggaa tcatcacgga agtctttccc atacatgaac gtggtcgggc attgggagca
     3601 ataggtgcgt ttgtttcatt aggctcgatt gctggaccag gtcttggcgg cttgattttg
     3661 tcccaatttt cttgggcgta cattttttgg atcaatgtac cggtgggaat cgtaacgatg
     3721 ttgattgctg aaaaattctt accaaaagat atcaccatga gcggccatac aatcgatgga
     3781 gggggctttg ctgccattgc ggctacgatt atcagctttt ttggagcaat ctttttgggg
     3841 caagaaagtg gctttttcca tccagtcccc cttgtactgt tcgtaatttc agtgattgcc
     3901 tttgtcagct ttttaaaagt tgaaaagaaa gcagcctctc cattgatttc tttcggtatt
     3961 tttaagaaca agatttttac catgagcttg attacagcct tgttgatttt ttcttccaac
     4021 ttctttgtca atgtagtgat tcctttttat ctgcaaaatg ctcgcgggtt gagtcctagc
     4081 tatgctgggc tgttgatgat ggtctttcct ttgatgatgg tcgtcggggc gcctttaagc
     4141 ggctacctga ccgaccgggt tggcccggag attttggtat tagtaggctt gggcttattg
     4201 tcggtaaccc aggtcatgta tatgtttttg tcagtggact ctcctttgtg gctatacatt
     4261 gtcgctactg ccatcatggg tgccggcaat gccttgttcc agtcccccaa taatacgatg
     4321 gttatgagct cggtcactat cgaaaacttg ggactggcag gctcgatgaa ttcctttgcc
     4381 cgaaatttgg ggatggtcag cgggatttcg ctttctacga cggtcttata tcaagcgatg
     4441 tctgctaaga tgggccagca agtaactaac tatatcgcaa atcggccgga tgtcttttta
     4501 tatggaatga agattacctt tttaggcagc tttgttttgt gttttgttgc ctttttcctg
     4561 actatctggc gtatcaaaaa acaaggaggt attcagaaaa atgtctgaaa aaaccactgt
     4621 tgaagaattt ttggaggcct gcgtatgggt gagtgggatt tccatggatg atggaagatg
     4681 aagtgaactt ctaaaagccg aaaaactcaa gtattatctt caaaatacta gtgctcaatg
     4741 agatgcttga tttgatcaca agaagactgc gaaagccgct ggcaaccgca agctaggtat
     4801 cccaccgatg gactacgttt tagaggctac tggttatgaa tacggagcca acgctctcat
     4861 ctgcataaga atacatcacc cagattatgt aaagattttc gatacttctg tcaaaaaatc
     4921 attaagatag ataaatgatt tccgtctagt caaattggtt cagaatgtag acacacagat
     4981 ggtaagaagt cactcacaat tcgagaatgg acttgtccta tttgccgtat acatcataat
     5041 cgaaacgtca acgatagtat caatattttg accaagggct atgaatacaa gcattgcctt
     5101 agaaaacatc aagaacctta ggaacaacgg ggatagcttg gttaataaga gataccgctg
     5161 tttaggcttg caagtatact cttttatcaa gaattccgtg gctttagtca tgagaggctt
     5221 aatactatga aaattttctt tagcattttt gatttaggtt ccttttctaa ttataataag
     5281 atgaccacta cagtaaccta ctgaaatata taaacagcca ctgtagtacc attttcaaag
     5341 tagtctatga gaggagaatc ttcattctta tgaacgctgg aattgcggat atgaatttaa
     5401 taaaaaaaac ccttaatgat tttacttcca attcaatatc aaaaggtact ggtatcaatt
     5461 taagcacaat aaaaaagctg aaaagtggtg aaagatcagt ggaaaagctg aatcttttag
     5521 atgctataaa aataacggaa tttgctatga aaaatggaaa agctgagatt gaaatatgga
     5581 gataattatc gtctagtgaa ttacttgcca cttagctgaa tttaacgatc cttggtgctt
     5641 gaagggggag attcttggga atagagcata cttgcaagcc tatgtcttta ctaacaacga
     5701 tatctcttat taaccaagct atccccgtag ttctaaggct tttactttta tctaagcaat
     5761 aatcgtattc aaaggaaatt tttgaccctt agaaagtctc attttatcag acgtgagctt
     5821 aggcattgct gaattttgtt aattcggttt taggttgtgc tttgagcata atatgaaagt
     5881 attctttatc acgatttcat tcatctaagg taatgtgatg tgattctgaa attctttcaa
     5941 aggcagtttt tgcataagct gacattacat catcaatgac atgtttacaa tgtttcacta
     6001 ctaaaataag ggggtaacca agaagaaaaa ctgaatgatt atttgtatct aattccatgg
     6061 tatatcaacc atttcctata tagactgatt atgacatgga ttaaaataaa aaggcactat
     6121 gtcttaattg tcggttttcg agttaccgaa tggcattcat cacccaccta tagaggatgg
     6181 gcgacttctg cctgaaataa gttacactac ttataacttc taataaaatt agattgagtc
     6241 attattattt aacatcaacg attttccagt ttccattttt ttgtaatact aatttgtact
     6301 gaaagtagct agtggttttt gatacattat caaggtattt tatctttgta ctcactagaa
     6361 aattttttcc gttttgtaga taaactggat tctctaactc cgaaaacttt aagtcaatat
     6421 tgattggtcg catcgcacca ttttcaacat aatactccaa ttctttttca gaagctgatg
     6481 gataaagttt aaaaaatgtt tctaaaaaat cggtgatttc ttccgttgtt ttggcatcaa
     6541 tttctgaatc gttttctact tgttttgctt catattttgc cttgtctggc tttccaatca
     6601 ttgttggcaa actagttact aaatgattcc tgttttcatc ttcaaagatt gtcactcgat
     6661 atgcagaaga aattgattgc gtcttttttc cctctgtaat atgttgctcc actaaaaatg
     6721 aaacgacaaa ttcttccgag tgtttttcaa catctagaat ttttacagat tgaacttcgg
     6781 aactcgtggg aatatctgct cgaaccatat cttggattaa agcgagaccg ttttctgtaa
     6841 ggtactgttc gagattactc attctctttt caattgcttc tttgttattt ttccatgaaa
     6901 aatattcttt cgcaaaatcc tctgtaaaac tttcaacgcc agaagtattc actaccttct
     6961 cttttataac cactttttca tgagttgtat gttgatcaat ggcggtgaaa tttttataaa
     7021 cgccaaaagc aagactacaa gacaataaaa gccaaagggc aagcaccatt tttcgatgct
     7081 tacccacgga tacaactcgt tcttttttct tttttgggct cttttccttt tgattccgtt
     7141 caatttttat cttcattctt tttcctcttt tctcttattt gtatcggcca gctccgataa
     7201 gatgctgccg ccaatagttt tccgttagat tcgtccaccc cactggatct cctgcatgaa
     7261 acatgcgatt ctcccctgcg taaatgccca catgggtcac ataagtaccg gcatcatagg
     7321 ttgaatgaaa gaaaactaaa tcacctggtt tggcatcttt tagatcaata tgagtcatgg
     7381 catcatattg atcctgagcc acccgaggaa gtttcaggtc aactttggca taacaccatt
     7441 gggtcaagcc actacaatca aaccccgttt ctggacttga gccaccaaac acataggccg
     7501 ttccttcata cttaaatgcc tcatcaaaaa tttcttggac tattttgtca tcaaactgag
     7561 ttgtagttag gtattgcttc actaataaac agtaaaacat attgccataa ttataccgcc
     7621 acccaccatt ttccttaatc gcgattgggt tggggtaatc gactttttga ccacctgatt
     7681 tttcctttga aaaagattca gctaaagcaa aactatgctt cttcccgttt ttcccgacat
     7741 gatctagata cccgccacca tagttatagg cctgaataat actatcttga tcacacccta
     7801 gttctttgga acgcttaacc aaagacgcaa aatagagaca gccttgtttg atggatgcct
     7861 cggtacttaa tgaattaggt ggtaatccgg ctgactcaga actttgcata acgtcttcca
     7921 ttgtgccacc agattccact tgaataatcg ccaaaagaat tggaatatac tctgttatat
     7981 ccgattcttt ggcatacttt tccaccattg cacggtgttt caatacttct tctgagacac
     8041 tagttccgcc aggagaagtg ccagaactac catttccctc actgtcttca ccaaacaaca
     8101 aacccacaca catcaaaata cccataaaag agatggtcag aaaagttaat gaaataacac
     8161 ccactttctt caagttcatt ttttctcacc tgaccttgat tttggacgat ggttcattct
     8221 tttttgtact ttttgattgg tctgctttgg cagagtggac ctctgtttca cttgttggaa
     8281 agaatttttc ttacttgctg attggctttt cccttttgaa atgatttctt gactagttag
     8341 ttttcgtttg atttcgggat tcagatttgc tctcacaatc cgctttggtg cttctttttt
     8401 cagcatcata tctttctgag gttgagctgc tttatctatt ggaatattcg cccttctttt
     8461 tccctgacga tttgttgctt cactcagtat cttgcgtttt tcgtccattt tcgcatttct
     8521 tcgttgtcgt tgtttttctc gcaaatccac tcgatccgct ttttcttgga acagtccgcg
     8581 cttgaattct tctggtgctt ttttcgactt ttcgattcct ttatggagat tgtatttcaa
     8641 attcgttggc gtattcctta cctgatcctt tacttgttta gtcttatctt tcaccaaagc
     8701 ttgggtatcc aaaactttgc cgatttttct cccagccaaa ttcattcggg attgtttctt
     8761 attatttgaa actggattct ccttagttaa gtcagatagt atttcgtgat cgttcggtaa
     8821 tcgtgaattc tttcgtagag aagaaccgga tggcttaaat ttggattttg attgttcctt
     8881 tgccaactta tttccgactg ctgcaccacc accaaaagcc aaagctcgtc ccacatttcg
     8941 attgagttgc cgcaattttc gattcattaa catttgcggt tttcgcatga cgcgtcttcc
     9001 cattgactgg ctatcattac tctggaggtt gaacatactc ataatgtcgc ccaatttgaa
     9061 atagataccg gcaaaggtca caatttgcag aaaagcaacc atgaaaaatg gataggttgc
     9121 cgaaatattg aaaaacatcg tggagatgct aaatgcagtc gtaatcacca aagtaacacc
     9181 cgctcgcatc ataatcgtat tgaataaacg aataatcgcc ttctttccta aactttcata
     9241 agtcggcaac atggacaata agaaactaat tggcagaaac atcgcaaaga taatgaagag
     9301 aatttgcgag aacaacataa tccctgttag aagaaaaaca aagaaggaaa tgccaatatt
     9361 gaacaatacc aaaaagacca cggtccctaa acggttcatg gtcttggtaa tactgagatt
     9421 cgcattgtca ttgtcttcaa tctctgcctt gaccgcttct tcccgatctt tgcctttgtt
     9481 ttcatctgga ctcacagata gaatcttgtt gacccggtct tcaccaattt cttctatgtt
     9541 ggaatcatca aattgcagta gtaaccaagg ttgttggact tgaatcgaaa acaaactatc
     9601 ccgaatcaaa tccacactat cttttccttg gctttctgaa ttgggtacaa cgatctcggt
     9661 tccaagggta agggccgctt cactaacatc tgagctaaag tcattgattt ttgtgatata
     9721 ggtaggcgca taagcaatga acgaacccga tagtagaaaa atcatcacaa aattaatcac
     9781 ggctcgaact gctttcgtgg tttctcgttt taataatccg gtatacgcca catacacccc
     9841 cagagctaaa atcatcaata atagaaaccc aaaataaaat ccggaggtct ggagtccatt
     9901 ttcagtaata ccagccaatg tttggatatt ctttccaata ctttcagccg tatccgaaat
     9961 gaaatctaat ttgtaagcct cttgaaccac ataacccgtg gcgttcgaca aatacaaact
    10021 caccgtccaa atgaaattag taatggcata aagaccatac atcacgcttt tccctaagcc
    10081 atcaccccaa ttccaaggca accaatccca agagctatcc acaaagaaat ctagttgata
    10141 gttgttcagt gaatatttgg aatatagatt tcctgcttca actgtatcat caactagccc
    10201 tgtcgcctcg gctaccgtac caactaaact caatagaact atcagagtga gtatgctaat
    10261 aaaaacgatt cccacaatcc gaaaaagttt ctttttcatc ccctcactcc attctctctt
    10321 gaacaggtgg acgggtatca aaggcatgga agagttcttc aaagactgga tgtacctgaa
    10381 ccacgcctac ccgaccatac aaatcctgca tcaagcattg gccattttcc aaatctcgta
    10441 ggcgtttttg attgctttca tcttctcgat ccaagccaaa gaattccaag gttttcttga
    10501 tttcatgaat atcggtactg cgaaacgcaa acttcaaccc gatattattc ttcaagcgtt
    10561 catccagtaa atcatcacta ttttgcgtga caaagtaaac cccggcattc atagcccgcc
    10621 cagcacgaac gagcttattc gataatgttt tcccttgcgc tacttgaaga aaactccacg
    10681 cctcatctaa gtctacaatt ttgaagattt ctcgattact atggataaag tccaaggcaa
    10741 aagtggaaat gacgattagc atggaaacac taagcaattc catggtggta tattcttcaa
    10801 aagtggtttc tgcatctggt aaaaccaaat ccgctacttg aatgatattt aactgcctat
    10861 ctaaactaat cgactgggta atcgaaccat ctgagaaaag caaatgggca aaatcataat
    10921 cggtaaagga ctcgatgtga tccgcgatat tttccgcaac tggtgagcct tcttttcgaa
    10981 gctcattaat tacttgtaat aatccccgtt gattgctttg ggtaaccgtc cgaatggcct
    11041 tacgcaacac tggaaacttc tccccatctc gactagaaat tcctgttaaa aaggtcagaa
    11101 tatcaattgc tagactttca gaatccttct ttttctccat aatcacataa ggatcaagta
    11161 atccttgatt ttttggctta ctagttaagt tgagaatatt gatttcatcc gcaatttctg
    11221 gcaaggtttc tttccagcca ccccgttccc cttttggatc gacaatcaca gcttgtccac
    11281 caaaaaggac ggcgtaataa actaagagat tgttactaaa ggactttccg ccaccgagtg
    11341 aaccgaggaa tgccgctgct agcgcattgg taactgaatc cttgacccct tgtgaggcca
    11401 atgccggttt caggtaaaca ttgcgccccg tatctacgtt ataaccaaag taaatgcctt
    11461 ccaactcccc taacatctga gtggcgccaa aaccaagtcc ggcgagaaaa tcagacgtaa
    11521 catactggat ataatcattc atgtatcgct tactagaagg taagaattct tcatgtagcc
    11581 ccatcatatc gccaaaaggc cggactagtt ttacattggt atcatcataa aaatctagca
    11641 cttcatcaca ccggcgcttt agttcatcta ctgattcggc acttactcga accacatagc
    11701 tcagtttgta catggattct ttggactgat ccaaggtagt ttccaattca tctacggaat
    11761 ccaatgcatc taagacattg gaatttgttt cattgtcaga ttgataggca tggttgtcca
    11821 agtcttttaa ttcctttttc ttattgcgaa cggtagcaag tgcctttttg ttcgtcacaa
    11881 tctccacatt catactagtg tcaattggga atgtaaattg ttgttgctgg taatagaaaa
    11941 gttctgagga gggaaactcc atttccccca caattgtatt aattgttaag tacgctacat
    12001 atgattcatg attctcatgt tccatgcata agtaccgggg cttttcttca atcaaacaac
    12061 gactgggacg taataagtcg taacgtttca ccaaagtttc cgacttcagc tttttctttg
    12121 gtagctgaaa ttcatattct tcaaatggtg tacccttttc gccatagata tgttcaatca
    12181 aatacatcag atcactcggt gtgactcgtc tgactttaaa tcttcgagcc aatttacttt
    12241 ccattagttt ttcaagcttg gtatatcggc gaatttcttc attggataag gaaacaaaat
    12301 cgcccatcaa atgatgattc actccataga caaattcttg aaagccggag aagaaagatt
    12361 tcttgagatt ctttagattg acctcttcat ctgtcgcaat cagtttgaag ccaataaaaa
    12421 agcgataatc aatctgacta tcgccaatca tggatactaa tgcctctgtc tgtaaatcaa
    12481 ttcgttgttt cgctacttct gttaatcgcc cggtaatttc cttttttgat cgttcttgcg
    12541 tggcacgaat actactttct gtggcaattt gtaaggcatg aatcttgcct tcacgatttt
    12601 gcgcaatcaa ttgacgaaag ttatcgtgga cttgatattt ctgttcaggt gacaaaaagg
    12661 agtaattgta aggtattaac tcatagtagg caaaacattc tccctcttga ttaaagacaa
    12721 gattattttc aatatactta atcgggtaca tagttcacac tccttaccag agtaatgggt
    12781 tcgtcccatc gtttcttctg ttctttgact ttctttcctg cgaaagtgac cttaaatcgt
    12841 aagtgatagc taataaaaga tcgtaaaaat cccaatggtt tcttaccgtc aaacgttttt
    12901 tgcgacatga accaagtaac tgccaccggt atacctaagt attttaaaaa ggccccctct
    12961 atcatagata gtggaggcac atgggcaaat acgatgacaa acagcaagga taaaacaaac
    13021 caagccatct ggttaaaagt cacaggaaaa ggcagttgaa agtcattaat tgcataaatg
    13081 accttttcaa ctgcccaaat actggtgtag ctttttattt tcttcatcaa atcattcctt
    13141 taaaaagaag gatagtttag aaatcaacta cccttcaata attttgttaa tatggatact
    13201 caaaaacacc tcgactcgtt accaaataat tgcctccaat ctccaagtct ctaccacacg
    13261 catcaaaatc aatatacgtc ttcaagtgtt ctggtacttc tccaaaatca ccagcttcta
    13321 caatcaagaa atatgctaca tcactcatat catcgcaatc tggataatag ataatatcat
    13381 ccacatgctc taccatctcc tcaaaagaat tgaagaaagc atgttgaatc tcagaagcaa
    13441 cttcaccaac tggcgttcct tcaagttcct ccgctaagcc acacaaccga ttaatttcct
    13501 caattggcgt atactcatca atctcaaagg gaagttcata atcatgaatt gcatattcct
    13561 cgtattcgtc atttaaccca attcgttctt tcacttcatc aaaatcgaca ggtggggtaa
    13621 accacgcccc caccaattca ccttcgttat atttcccgag gttcgcaata taaacacgca
    13681 tctgttccat cgtgcttccc ccttacgcac cgacaattcg gttaaacaaa ctgagtaaga
    13741 catccttcac cccagaagca ttaaagacca atccgaccgc aatcaaagca atcaccaaaa
    13801 agccaatcag tttggaaaac tcccgcttaa atcctaaaaa caaaccaatg accacaattg
    13861 ccagtaagac taaggattgg gcattggtta aaaaccattg atatatggat aggtaagttc
    13921 ctgatattat aaatatctca aacttttccc cccattcaca cggagcgtgc gactttcacc
    13981 gcactccgcg ttccatcgtg tttatattga aaagtattta tgtagtagac ctatatcaag
    14041 tttaattctt gattgccagc taatactcta tatttattta gtcttatgag ctggacttta
    14101 tttagcttca atagagctat atatttttgt attgtttctt ctgttttagc gtgtattaga
    14161 cggtgtaccg cctcttttac caaaataaga tttttataat cgtcatttcc accagctcca
    14221 gttggtacaa tatgatgaca atgtatttca ttagcttgtt gaaattcttc cagagttata
    14281 gcacattttc catgttgagc agaaaatagc gaaattcgat tgtctgcgta ttctattgtg
    14341 cgattttcca aagataattt cattaactgg tacatcagta atttatcaac acttagattg
    14401 gtgtggataa attttcttcc ctctagagtg tagcaattcg ctaaatacac ctttgccatt
    14461 ggttttctgt gttggatata tccaacaggg aaaataggtt cgccctcgcc agcaacatac
    14521 cgaagcattt tagatttacc atatcttgtt gcctcctttg tcgtaagttg tctgcctgtt
    14581 tttctcaatc tgcccctttt gtttgtacct aatctgttag tgaagatagc cattacagct
    14641 ctattcagtt tcatacaatc cagatttaca ttcgttgcaa tctgataata attttgcata
    14701 cctaatacca ttgaattata aagtcttgtt tcttctaatt ccgttttccc ttttgaggga
    14761 gaagcaattt tctttgcctg tgataccagc ttgtcttttt ggtttttgaa ttgtttatca
    14821 cttatatgtg atttaacaac taacttcttt cttctcggta tcattttaat cttgaaacca
    14881 agaaagtcgg agtatctttt tctaacattt acaattcttg ttttttcttg tgatacttcc
    14941 aattttaatc gttgttcaat ccattgagtg attgctattt tggctttttc agcactttcc
    15001 ttgtagcggc agaaaactct aaaatcatct gcataacgaa ccatatacat ttcctttaac
    15061 tttgttttct tcatttcttt gtaagcgttg cttttacatg ggctaccgct tttattgata
    15121 tgaaccacat agttttttgt aacaggattt tcctgccatt ggctttctac ccaatggtct
    15181 aactcattaa gaacaatgtt tgctagtagt ggggatatta ttcccccttg cggagttcct
    15241 ttctctggat atgttattgt accgttagtc attttgattg gagcttttaa tatccttttt
    15301 aaaatgaata tcaagtgctt gtcccgtatt cccatagccc atatctgttt tatcagcttg
    15361 gaatggttca cgttgtcaaa gaaaccctta atatcaaatt caatcacata atgtagctgg
    15421 ctgatttgta ggcggttata ggttgctttt attgcatttt caactgacct attaggtcta
    15481 aaaccatagc tgttttcact aaatttagct tcgcagattg gttctaatac ttgttttata
    15541 cattgttgta taagcctgtc ccacatacaa ggaataccta gaggtctggt tttaccattt
    15601 ggttttggta tttcttttcg cctcactggc ttaggtcgat acccatgtgg actgcctgca
    15661 acaatgtatc ttactttttt gacaactgtt tctgctggca atttgccaat atctttaatt
    15721 attgtaccgt ctgttcctgc tgttttactg cctgcatttg ctttgatatt tcgatatgcc
    15781 agtaatatat tttctctcga taggataata tccataagat gagtgaaaat atctccattc
    15841 ttacttttgg catataaatc atcaaaggtg ttttgcattt cgtaatattc agcgtgtctt
    15901 aaatcttcta cacacaatgt ttcattttta tttttaggca taaggcttca ctcctttact
    15961 tgaaattgag tgagcttttt tatcttactc gtaatcctta ttgaaacaac ataatacttt
    16021 tctaatatat acacgacttg agggcattcc tccattctca ttacaagaat ttcttaggtt
    16081 cgccctctgc ttttcagcat tagttcaact gcttattgtt cttcgtcagt ttattctctt
    16141 gatgagaacc caataccttt cctcgttccg ataatcttat ctatacatat atatccttag
    16201 gtcactagct ataagcctgc tggcttgata ccgcctgtaa cgttatatgg catttcataa
    16261 cctatattct tactttgcca caccagctac gatttctcgt acctttgcat ttctacaaag
    16321 ttcaaattta gacccgtaca ttcgctgttt cgtcagttcc ttatagaaca ttctaaccat
    16381 agatatttta tagacccccg acctgttgga tacgctaccc acctctggat agttttcgat
    16441 agaagatata tgttcatcaa ctattacggt atctttcagc cgtctttacc gagcttcaaa
    16501 cattctgagt ttcctcatac aatgcttgtc ggaggctcag ggcaggcgtt tcagagcgtt
    16561 acttcatcat tccacctgtc agattatcag ttctcctaat aaccaaactt tttcgttctt
    16621 ttattagtgc cttagctttt cactaaggaa cgagtcgcac ataaattttg tccgaaattc
    16681 atcttgtcac tttcctttct tttccattgc tcgatccgct gctgcacttg cattcatcaa
    16741 gcacatcaca gtaacaccaa gcatactgcc accaaatacc aaaaaaatat cttttacaat
    16801 tcccatgacg attcctcttt tcatataatc aaattttcaa ttgccatact ttgttgctcc
    16861 aaaatctttt tatgtcggtc tgataagcgt gcattctgta ccatatcctg gatataggtt
    16921 gtccgattca atcgatccaa ctccttagcc attttccacg taggggcgac ttgtcggcct
    16981 agccaattca aggttcgttc aatggtatac ggttttggct cggaagttaa ttgaatctct
    17041 ttccgattcc gtccaataaa acgcccccat tgttcattca agggccagtt ggttcgcctt
    17101 ttaccttcta ctttatccgc aaatcgcaag taacgattga taatggaaaa cgtggtgctc
    17161 tcaatgctac gaccttttaa caaatcttga atcgcatgaa aagcacggtc atttttcagt
    17221 cgaatctcaa aacgattttt cgtctcagtt tggtctaatg gaatccctaa cttgatatat
    17281 tgttcatagt ctttttcata caaacagaaa taaacctcac tcttgagact gccgatatat
    17341 aaggtattgc ccatgccgtc cttttcatca gcctttaaaa gttccccgga acgataactt
    17401 ttaaaggtac gaaacaagga gatacattct tccttctgac attttttcgt tagctcaggt
    17461 atatccaata gccccactcg atcattgatt gccaagtcca agcgtttcat cacgccaccg
    17521 gcctttagac aatcctcgaa aaagtcgtac cagctacgct tttgagcgag taaaaaagtt
    17581 tcaaattgtc ggcaacctcg cccctttaat tctaaaagca cacctttatc ttcttcatga
    17641 gacaacatca ccacaatatc acccatgaca tactgttctt gataggagta aaaggcataa
    17701 tcttcatgga gcatatactc aaatttcaaa tggagaattt cttgaataat ttttcgtgca
    17761 tccgttgtgg gaaagcgaat ccttacatag tcaaacagta acgtcaaagg caaatcggga
    17821 ttaaagcttt ccagttggtc aaaaattttc ctcatggttt ttatagatgc ggttacgcca
    17881 ccattttcta tctgatttaa gtacggccga ctaagtccac aagccgtagc caatttattt
    17941 tgtgaaacgc cgtattcgtt gcgtcggtct tttaatagcc gataatctaa attccgttgt
    18001 gccaagttct cacctccaaa ttcaaggtgt taactttttc aaatgagtta acacctatac
    18061 aaattactta ctatctttga tacaacaagc attcttaatg gttgcttact tattttttgt
    18121 gtttttgtac ccccctgtta gatacggggg tgagacatca tggtcggcta acgccgtcca
    18181 tgaacaggca cagcaccacg gaggatttct gcttcgcgaa tcctccgcgg tcagcgccta
    18241 acagtcagtt gtgttggttt aggttactca tctgtaatac catttacttc caactctgct
    18301 ttcatttcta acaagtattg acgaatatct ggttcttttg cttggtccgc aatccgctca
    18361 aattctgcaa ttacttgatt tatttcaatt cgagcatccg tctcctcatt cattaaatat
    18421 ggatcgccag cgactttgac accattcttg cgaatatcaa attttaaccg tcgcaattgt
    18481 tcttctaatt ctgggtctga ctgaatcttt ccaatcaccc aataaatttc tccaatcaac
    18541 tcatcataaa ccatgtacat ccaactcttt taatcttctg gcaatatcaa aaatgaccgg
    18601 aactgttaca gagtttccgg cttgcttata caactgacta tctgaattga cttgagaatc
    18661 tcgatcaaaa gcccaatcgg gaaagccctg aagtcgccaa cactctctag gtgttagctt
    18721 ccgaatttga tactgatccg tgacgatcgc ttgttggtct cctgttagta aggtatgaac
    18781 actctgtttt cccagacgac ctcggcgact tgtggaatta ggaaaagcca gattcacact
    18841 gtcttctggc ttcacctctt gatattctgt ttcatttttt cgaatcatga tttttgtacc
    18901 accttgtttc gttgacaagg tgggggcggt tccagtaact tcataaactc gattaccttg
    18961 ttcaaacttt cccggtaata gtcctgctac agagattcca tcccttgctg gcagtgcgat
    19021 tttgattcct tcatcacctg ttaaggttgg cgctacgcca tctgaaccat agacttgtcc
    19081 acccattcct cgattggaag gattgatatt taccaaattt ctgatgtttt gatcagatgt
    19141 tgaagacggt ccaatgatag gaaatacttg ttcggtacat gcgtctcgag aatgtccgat
    19201 aaggaatatc cgctcccggc tttggggaat gtaggccttt gagttaagca cctaccactc
    19261 cacgtcatac cccaatccat ccagcgttcg gaggatcgtc tcgaacgtag cccctccttc
    19321 gtgattgagc aatcctcgga cgttctcaag gaataaaagc ttaggtcgga gaagagtggt
    19381 gaaccgagcg atttcaaaga ataaagtacc tcgagtatcg gaaaatcttt gccgctttcc
    19441 cgcaattgaa aaagcttggc acggaaatcc gccgcaaagg atgtccactg gtccgaggga
    19501 ttgaataaac tcatctgata cacttgtgat gtcatgcatt tccacctcct tacttgtatc
    19561 atgaattgct ttataactac gtctagcaaa tttatcaatt tcacaaaaac cgatacaatg
    19621 atggctcgcc tgttccatac ctaaacgaaa gccaccaata ccggcgaata aatctaaaaa
    19681 tctcatacat tacctcattc attgcaaaga aaaaagccaa gaacattttg ctcttggctc
    19741 atacattatt aatt
//
LOCUS       DAHHZJ010000038        19695 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.039, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000038 DAHHZJ010000000
VERSION     DAHHZJ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.039"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            70..717
                     /locus_tag="NBL02_001108"
     CDS             70..717
                     /locus_tag="NBL02_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCD2571808.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            801..1745
                     /locus_tag="NBL02_001109"
     CDS             801..1745
                     /locus_tag="NBL02_001109"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HCD2571809.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            1880..3331
                     /locus_tag="NBL02_001110"
     CDS             1880..3331
                     /locus_tag="NBL02_001110"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HCD2571810.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            3352..4188
                     /locus_tag="NBL02_001111"
     CDS             3352..4188
                     /locus_tag="NBL02_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCD2571811.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            4188..4847
                     /locus_tag="NBL02_001112"
     CDS             4188..4847
                     /locus_tag="NBL02_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCD2571812.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            4973..5662
                     /locus_tag="NBL02_001113"
     CDS             4973..5662
                     /locus_tag="NBL02_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571813.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            5668..6624
                     /locus_tag="NBL02_001114"
     CDS             5668..6624
                     /locus_tag="NBL02_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HCD2571814.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            6668..7180
                     /locus_tag="NBL02_001115"
     CDS             6668..7180
                     /locus_tag="NBL02_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD2571815.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(7436..7984)
                     /locus_tag="NBL02_001116"
     CDS             complement(7436..7984)
                     /locus_tag="NBL02_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCD2571816.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(8073..9254)
                     /locus_tag="NBL02_001117"
     CDS             complement(8073..9254)
                     /locus_tag="NBL02_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCD2571817.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(9490..10998)
                     /locus_tag="NBL02_001118"
     CDS             complement(9490..10998)
                     /locus_tag="NBL02_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HCD2571818.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            11157..11348
                     /locus_tag="NBL02_001119"
     CDS             11157..11348
                     /locus_tag="NBL02_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571819.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            11551..12468
                     /locus_tag="NBL02_001120"
     CDS             11551..12468
                     /locus_tag="NBL02_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HCD2571820.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            12461..13534
                     /locus_tag="NBL02_001121"
     CDS             12461..13534
                     /locus_tag="NBL02_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571821.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            13682..14200
                     /locus_tag="NBL02_001122"
     CDS             13682..14200
                     /locus_tag="NBL02_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571822.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            14181..14573
                     /locus_tag="NBL02_001123"
     CDS             14181..14573
                     /locus_tag="NBL02_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HCD2571823.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            14649..15584
                     /locus_tag="NBL02_001124"
     CDS             14649..15584
                     /locus_tag="NBL02_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HCD2571824.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            15717..16496
                     /locus_tag="NBL02_001125"
     CDS             15717..16496
                     /locus_tag="NBL02_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HCD2571825.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            16489..16806
                     /locus_tag="NBL02_001126"
     CDS             16489..16806
                     /locus_tag="NBL02_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HCD2571826.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            17050..18660
                     /locus_tag="NBL02_001127"
     CDS             17050..18660
                     /locus_tag="NBL02_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCD2571827.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            18817..19665
                     /locus_tag="NBL02_001128"
     CDS             18817..19665
                     /locus_tag="NBL02_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCD2571828.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         6364 a         3519 c         3962 g         5850 t
ORIGIN      
        1 atgacagaaa attttttctt cgttatactt gtaatagaat actattttat aacaaagggg
       61 atcgaatcaa tgaaaaaaac tgttatttat actagtttat ttgcagcttt gtttttagct
      121 gcttgtcaat caaataccaa tcaaggcaca tctgggaata cgtcttccag tagcgaacag
      181 cgctcttcac aggtatcgga ctcctcaaca aaagcaagca catcttcgtc tagccagaca
      241 aaaagttcta gtgaagcaag tactcagtca gatatcaaag tgtctgcagc tcgtgcgatc
      301 gaattgttcc aagataaata cccagatact gcaattacta gtttggaatt agatagtgat
      361 tggggatctt acttctacaa gatagaaggt gtcgatgatc aaaaagaata cgaagcaaga
      421 attgatgctc aaaatgaaaa aatggagaca gagaatccag aaactctaga tcgagatgaa
      481 caaaatggtc aaaagaaaaa agaagatggt ctggatgttg ataaattgat cacgattgag
      541 gaagcagcaa aaatcgccgt cgacaaagta ggaagcggta cggcaacaga ttgggatttg
      601 gataaagatt taggtactac ttattgggaa gtcaaagtaa aagacggcaa tcaaagtaca
      661 tcagtcaaga tcaatagcca aacaggcgaa gtactgtcaa ctgaacaaga tgattaagaa
      721 tttcttcgga aaacaagagt tgatttttct tttagtcatg atacactaga caaaataact
      781 tgtttgaagg agcgatttac ttgagtttag aaacgacctt tgccaatcat atctttgcca
      841 atcctttgat gaatgcatct ggtgtccact gcatgactac ccaggaatta gatgaactgg
      901 ctcattcaga agcaggagca tttatcacta agagctgtac gatcaatgaa cgaaaaggaa
      961 atcccgaacc acgctatttt gatgttcctt tagggagtat caattctatg ggacttccaa
     1021 acttaggttt ttcttattat ctagagtatg ctcttgcgta tgaaaaagtg caggaaaacc
     1081 aaaatcaacc gttatttttt tccattgccg ggatgagtgt ccaggaaaac ttggaaatgc
     1141 ttgagaaaat agaaaaaagt gggtttaatg ggatcactga attaaattta tcttgtccca
     1201 atgtgcctgg aaaaccacaa ctagcttacg attttgaggc cacttatgaa acactgaaag
     1261 aagtattcag catcttttct aagccgctag gaatcaaatt gcctccttat tttgattttg
     1321 ctcattttga tcagatggct gatatcttga atcagtttcc attgacctat gtcaatgcta
     1381 tcaacagtgt gggcaatggt ttgtatatcg atacagaaca agaagcagta gtcatcaaac
     1441 caaaagaagg gttcggcggc attggcggag agtatatcaa gccgactgct ttggcgaatg
     1501 ttcgcgcttt ttatacacgt ttgaaaccag aaatccaaat cattggtaca ggtgggattc
     1561 ggacaggaca agatgcattt gaacatctgc tatgtggcgc aagcatgtta cagattggta
     1621 ccgagcttca taaagaaggg ccggaaattt tttctagaat cataaaagaa ttaacgcaga
     1681 ttatgtcaga aaaaggatat acgtcaattg acgaatttaa aggcaaatta cgcaccattt
     1741 catagcaaag agacgaaggt catgattgat cttcgtcttt ttttataaaa aacactcgtc
     1801 tgttcattcc tgtgcaagcg tactcattgt atgttagacg aacttgtggg aaaataacag
     1861 aggcaaaaag gaggataata tgaatgatgc aacagaatta gcaaaagagc ttaaagaaaa
     1921 agaatttagt tttcaagaac tagttacgga tatacagaaa aaaataaaga agcaaaatca
     1981 tgaattgaat gcgtttgtcg cgcttgaacc attcgagagt aattcccacg agctattgat
     2041 tccccagtca gaaaaagggc cattggctgg tatcccattt cctttaaaaa tgctcggtca
     2101 agaaaaaaag ggatggctag ctacttcagg atctcgttta tttgaaacac accgagcttc
     2161 tcatacatct aattatgtgc ggcaagcaga agcgataggt ctagttcctt ttggacaaac
     2221 caatgcgcca gagtttggtt tcaaaaatat tacagaccca gtcatctatg gcccagcaag
     2281 aaacccttgg aaccttgaat gtacaccggg cggatcaagt ggaggagctg ctgctgcagt
     2341 ttcatcagga atcgttcctt tagctggtgc tagtgatggc ggtggttcta ttagaatacc
     2401 cgcttcgttt tgcggattga ttgggctgaa accttcgcga gggacgatgc cagttggtcc
     2461 atatgcttgg agaggatggc aaggagctgc tattgatttc ggcttgacag tttctatgag
     2521 agacacggag gctttatttt atgggatgag aagtatccat tccggtgcgc catatcaagt
     2581 atcgccagtt gagtggcaaa cacatccaag aaaaaaacgg ttgaagatag ctttttgtgt
     2641 tgattcaccg atccactcac agattagtga agaggcagta cttgctgtaa aaaaagctgt
     2701 agatttcctt ggaaaagaag ggcatgattt gacagagatt ccttacccac tagatggtcg
     2761 taaactgatc acgtcttatt atcagatgaa tggcgcagaa acagcagcaa tgatccgctc
     2821 gattgaaaca ggcatccagc gagctgtcag aaaagaagaa cttgaaccca tttcttggac
     2881 tttgttccaa tatggtcaga agctctctgc cgctacttac atcgattctc tgcatgtatg
     2941 ggatgaagca gcaattgtca tggaaaacct acatcaagaa tacgatctat tcctttcacc
     3001 aacgactgca tttaccgccc ctaaaatcag cgaagatttg caaagtgatg cgataagagc
     3061 acagatggaa cacatcagtg attgttctga agtagaaggt ttagacgttg tttcttccat
     3121 gtttgaggag agcttgaaga tcacacctta tacacagttg gctaatctga ctggacaacc
     3181 agcaatcagc ttgcctactc atttgacaaa agaaaacatg cctctaggaa tccaatttat
     3241 ggctgccaga ggaagagaag atttgctatt tcaaatcggc tatctatttg aagcaaatca
     3301 tcagttccat ttaccaaatc catggaaata gcagaaacaa ggagaagaag aatgaagaaa
     3361 aaaagattgt ttgctctaat aactagtgcc ttttttattt taagcgcttg cggtacagct
     3421 gccgctaatc aggaagataa cagctggagt aatgtagaaa aaaagaaaga attagtagta
     3481 gcgacttctg gcacactgtt tcccgcatct tattataatg atcaaaatga attagttggt
     3541 tatgatgtag atttggcaaa agaggtggct aaacggttaa atgtcaaaat ctcctttaaa
     3601 gaatacaacg tggatggaca aatcacttct gtttcaaaaa gagaatcaga ttttgctgcg
     3661 aacgattttg gtatcacaaa agaaagagaa aaaaaattct ccctttcaga gactttcaaa
     3721 tattcatttg acagtatgat cgtacgaaaa aaagatgatt caggaatcca ctcacttgaa
     3781 gatcttaaag ggaaaaaagc agctggcgaa cccaatacta gctatatgcg tttagcggaa
     3841 aaacacggag ctaaattagt cacatatgac aatgccacga atgatcagta tttaagcgat
     3901 gtagcaaatg gtcgaacaga tgttattcta aatgattatt atctgcagaa aatggccgta
     3961 tctgctttac cagatatccc tgtaaaaata ctggagaacg tttatttcaa tgaaaacgaa
     4021 agtggttttt tattgaaaga aggaaatgat gctctgaaaa agaaaatcga tggcgtatta
     4081 aaagagatga aagaagatgg gacgacaaag aaaatatctg aaaaatattt tcaaactgat
     4141 gtttcagtca aaccgaaaga aacgatggct gagtcggtga gtgagtgatg tactttcctc
     4201 taattgattt ttccctcatg tggacttcac ttcctttcgt actgcaaggc ttggtataca
     4261 cattagggat tggcttcgtg tcgtttcttt taggaaatct ggtagggttg cttttgacag
     4321 ttttaggtct tttggattgg ctgcctctga atgtatttat tcgtttttat ttatcgttct
     4381 ttagaggcat tcccgcacta gtcctgctgt ttctgttata ttttgggtta ccttatcaat
     4441 taagtgcctt aacggcttca gtcatctgct ttacgattac cagcagtgca tttattggtg
     4501 aaatctatcg aggatcacta gcaggcgttt cttcaggtca gtgggacgct ggtttagctt
     4561 ctggtttttc tttctttggg gtattgaaat atatcgtttt gccgcaagct tttcgaatct
     4621 cagtaccggc actcagtaac gtagctatgg atctagtgaa aggtacttcg ttagcagcaa
     4681 caatcacagt gccagaaata tttcaaaaag caaagatcat aggcggtcgg acattcgatt
     4741 atttttctct ctatatttta gttgcactga tttattgggt catttgtatc gggatcgaaa
     4801 aagtgcaaca gtatttagaa aaacgttcta tgcatcagtc aatctgaaaa atagaaatgg
     4861 tgctagcgtg gctggcacca tttctatttt ttgtttcttg tatattctgc ctagtatttt
     4921 aaaaccattt catgtaaaat gtaaatcgaa aatatgttcg gaggtagaaa acatgaagac
     4981 cccgctaacc atcgaaatag aatcctcgtt ataccaatat tgcatcgagc aaggcggctt
     5041 ggttgtagaa gaagtcacga tgcctgatga ccaaggaatt gtagatacgc ttagctgctt
     5101 gacaaaattt gatggaagcc gagaatggcg atgctatgag ctgaaagtga caaaagcaga
     5161 ttttcgctct acagccaaac tgtcatttgt tggacattat aattattttg ttttaccaag
     5221 taaactatat gaagaagtaa aaactgaaat ccctacagga ataggtgttt tagtgtatcg
     5281 tccgtacacg gttgaagagg agatgcctgc ctctggtaca tttctgattg cgaaaaaagc
     5341 agcaaagaaa gagcttgctg taccggaaga agctttgaca aaccgcttca tggcttcttt
     5401 attcagggaa gtaagaaaag caaagcaaat ggagcgaggc gtaaaatatt ttccaacaga
     5461 tcaattgtac aaagaattga aaagacgttc agaaaatcaa gatccatttt ctaacgaacg
     5521 atattatgaa cggtttttag aggaaatagc tagtcaggca atcaatgagt tgcaagaaga
     5581 agttatcgcg cttcagcatg attatgaata tttgaggcaa caacagcaga tccgtcggtt
     5641 gcctacagag ccattggagt gaaaaatatg tactatcctt atttaagagg aaaacaattt
     5701 gacctattag cattaaaaga agcactgtcg cgaggattgc tctccaataa aatccaacca
     5761 gtgatcgaac cagtacgtga ttccgctacg cttaaaaatg tgatcgaatt atttcaaaaa
     5821 aagcaccatc caatcgctgt gatccaaaat ccccaagtag gacaattcaa attattcgat
     5881 cagcatgtgc acagttggac agtcaaagaa aatagctctg tcgtccctgc acagatctta
     5941 acgccagaaa atctcagtga agtactggat tcacctccag cgtttcttgt tttcgatgga
     6001 cagcactacc caaaagatac agaggtgtgg aaacagttgg caggagttga ttcgaaattt
     6061 ttgataccag atactagtcg ctttcgcatc tggctgcccg aaaacaaaat tgtgatccgt
     6121 gactcttttc aaacgagaaa gcatgttgaa agttatgcag ataaaacaga tgactttttt
     6181 agtgatgact atcttttttt ccacgcagac ggatatattg gattttctga tttcacgatc
     6241 gaaggtagcc gttatttcga taaagggttc cctagccgag cgcttgctat ccatctaacg
     6301 tatattgatg cttacggcaa tatacgcgtg aaacattttg tctctgacag taacgactct
     6361 gctaaagacc aagcattgaa gtttcttgaa gcaggagata aaatgaaaaa atggatcatg
     6421 cgtcaccatc atcaactctt gattacttca ggaatgattg aattattggc tctttaccag
     6481 caacaaaaat tccctggact gggagtgttg aaaaagtggt cacttatgca tcatttggaa
     6541 cttattagtc acctgctaga tcatccaaaa gattggctta aatattattc aaaagttcag
     6601 gaactatatg aagtgatcca atgattagat aatgtaaatg aaaaaggaat agaaaagagg
     6661 aaactggatg tatattcggc aaacaaggaa agaagaagta gaagaagtgg tagaaatcat
     6721 taatgccgca gcggatttca ttgcttcgca aggctctcct caatggcaaa acgggcagaa
     6781 accaacaaaa gaaacgattt tgaacgatat tgaaagaaaa gaaagctatg tattaatagt
     6841 ggaagatcaa attgctggaa cagctgcatt agtctcaagc gtggatcccg tttacacaaa
     6901 aatagatggt agttggaaag aatcaactgg tccttattta tcgattcatc gtgtcgcttt
     6961 agcagaaagt actcgaggaa aaaaactagc aaaaccgttc ttagaacatt tgttgacgat
     7021 tgcagccagc aaaaatatcc aagatatccg tatcgatact tatccagcta atgaaccgat
     7081 ggaaaagacc atctattctg ctggattcac ttatcaaggg atgattgaat ttccttttga
     7141 acacggcgaa agaaaagcat atcaaataat catcaattga ctataaaatc atctgagcaa
     7201 agataaagac attaacacta ctgaaaaaaa caatcatatg atagccaaga ggttgtaaca
     7261 gaagtggcca gcttcaaaaa acaagaaaga atttccgaaa atgcttctca aatttttgtg
     7321 gaattttagc gtattttacg aaatagctgc tttctgaaac tcccttcatc gagaggtcgt
     7381 gacatagctt ttgtcacgac ctctcggctt atcctacgag tattattcga tacatttata
     7441 tcaataatcc tttttttatt ttatctgcag cttcttggaa cgctccatcg tttgacacga
     7501 ctaaaagtga ggggatagct ttcaaacgtt tcaactgtgc taaatcttct acataaaccg
     7561 ccagtctggc tgaaatctgt tcagatgaat ctccacgttt tttcaggcgc gattgcaatg
     7621 tttctttaga gatcgtaata aagataggac ggactaccgt cccgaaatac tcatataaat
     7681 gccaaaaacc atttgctgtc aaaacggcgt aacaatttcc tactgtcagt ttctttttaa
     7741 tttctgcttt actggaacca tatttatgtt gagcatattg gtcccactct gcaaactcat
     7801 ttcccttgat catctgattg aatgtattgt cagaaacaaa ataataatcc tcttgatctt
     7861 tttccccagg tcttggtgat cgattcgtgt aagtaatgat cttttgttcc ggctgaaaaa
     7921 tagcttctgc gagtgaagtt tttccagaac cagaaggacc gataaataca tagcaataat
     7981 tcattgtcat ttctcctttg atgattgaac aagtgaaaaa gctttggaaa atatttccaa
     8041 agcttttaaa agtaactttg cttttttgta tattatacca gcaagccagt cacaatagca
     8101 cttaaaaagc tgactaaagt cgctgttagc aaaattttca atccgtgttt tgcgacaaga
     8161 tttccttttt cttcatttaa tcccttcatc gcaccagaaa tgatcccgat agaagagaag
     8221 ttggcaaatg aaacgaggaa gacagatata attgctgttg tacgctcagt aaaatggaaa
     8281 ttaccatttg ctaattctgt catagcaaca aattcatttg taaccagttt agtcgccatg
     8341 atactaccgg catccactgc ttctttccat gggattccgc tgatgaaggc aagaggggca
     8401 aagatgtaac ctaggatctc ttggaaagtg atgccgaata tcccgtggaa gatagcattg
     8461 atcatggcaa tcaaagcaac aaaaccgata agcatagcag caaccgtgat cgccacatgg
     8521 aaaccatcaa gaatgtattc tcccaacatt tggaaaaatg tctgttcttt gttttcttca
     8581 atgatcagct catcttcttt ttcttctaat acatagggat ttacaattga agccagaata
     8641 taaccgccaa ataagttcaa aacaagggca gtgatcacat atttagaatc gatcagtgcc
     8701 atataagatc cgacgatcga catagaaact gtagacatag ccgatacact catggtgaac
     8761 aaacgtttct cagataggaa gggtaattcc ttttttatgg aaataaaaac ttccgattgt
     8821 cctaaaatag cagaagctac tccattataa gattccaatt tgcccatccc gttgatttta
     8881 cttaatccta gaccaacagc tttcataaat aatggaagaa tcttgatata gcgtagaata
     8941 ccgataatag cagaaataaa tacaattggc atcaatacgg aaagaaagaa aggtgcactt
     9001 cctttatttg ctacaccggc aaaaacaaag tcaacacctt gcccagcaaa agcaagaaga
     9061 tgatcaaaca tttttgccaa tacagaaaca accgctgtac caaaagttgt acgtaataat
     9121 acgaaaccaa gaacaagctg taaaacgaac atcacaatta gtggtttcaa tttgattgct
     9181 tttttgttac tgctgatcaa aaaagcaagc cctaatgtga caaataaacc aataatacct
     9241 aataaataac tcaaataatc actcctattc caaattccgt tttattttac ctgttttgca
     9301 aataaaatac taatctttag tgaaattttc attacttttt tagagtgaaa cgcattcatt
     9361 ttccattaga aaataaagcg aacaaaataa aaggtttaaa aagtgtaacg tcttagtaaa
     9421 gtctataaaa aaacgaaact atcgattaag ctaatagttt cgttttcaac atttataagt
     9481 aaactttttc tattggtaat atagattttc ggaagggtag acaggtgcgc aggtaatatc
     9541 gattcctagt tttttcaatg tttgttcatc atttttattg accatcacag tagagtgggc
     9601 ttgtgctccc tcaagttcag ctagtttatc ataagcaact tgagcgattg gattcgttac
     9661 tgcactgatt gataaagcga tcaagacttc attgatgctt agtgcatcca aagaggaatg
     9721 aagtgtcttt gatttcaatc cttgtattgt ttcaagaact aatggtgaca ataaaggaat
     9781 gtcatctgcg atatgggcta aatgttttac tgcatttaag ataactgccg ctgacgcatc
     9841 catcaatgaa cttgtacgcc cggtaatgat gacttcatct ggtaattcga ttgcaatcac
     9901 agcagaagga tcttttgtag gactttgcgc ttgaatctta tctgcatagt catgggcagg
     9961 gactaccacg tttcggtctt caggacgtaa accgacttct tccataatca gtttgattcg
    10021 attaactgct tcttcgtcga tcaatccttt tttgaagtcg cattctgttt ggaaataacg
    10081 acggatgatt tcttgtttag aggcttcttg gacaacggta tcatccacga tcccaaatcc
    10141 tacacgattc acacccatgt cagtaggaga ttgataaaca gattctttac cagtaatttt
    10201 ttcaataata cgtttgatta caggaaatgt ctcaacatcc cgattatagt tcacagctac
    10261 tttattgtag gcatcaaagt ggaaggaatc gatcatattg acatctttta gatcgactgt
    10321 ggctgcttca taagcaatgt tcagcggatg tttcaacgga acattccata ctgggaaagt
    10381 ttcgaacttg gagtaaccag ctgcatgtcc tttttggctt tcatgataca gttggttcaa
    10441 ggaagtagcg agctttccac ttcctgggcc aggagctgtc actacaacga ttggtttagt
    10501 tgtttcaata tactcatttt ttccaaaacc atcttctgag acgatttttt ctacgttgac
    10561 tggatagtct tcgatttctg agtgtttata gaccttgatg tttcgtcttt ctagtttatt
    10621 gataaagact ttcgtggcag gctgtccact atagcgagtg ataacgacac tgttgattaa
    10681 taagccataa ccacgtaatt catcgattaa ccgcaagata tccatatcat aagtaatccc
    10741 ataatcacca cggatcttat tacgttcaat atctccagca tagacgcaaa tcagtatttc
    10801 agcttgatct ttcaattttt gcagcaattt gattttcgca tcttcttcaa atcctggaag
    10861 tacacgtttc gcatgcttgt cgtcaactag cttcccacca aattctaaat ataatttgtc
    10921 atagtggttc actcgttcca aaatatacgc tgactgctct tcaatatatt tttctgaatc
    10981 aaaaccaatt tttttcacta ggttccctcc ttaagtacta aagcattttt tcattttttt
    11041 gaggattcgt caactggaag agcgaaattc ctcaaaaaaa caaagaaaat ggtttaaacc
    11101 aacttgtcta gatagttatt cattggtatg atagtttttg ataaggaagg tgctacatga
    11161 aaaaaagaca gaaaaagaaa aacgcatata aacactatat ccgttcgatt tttaccggtt
    11221 atgaaagaat gcttgaagat cccgaattgg aacaattgac gtttacttat ctaaacgaag
    11281 aaacgcagct aactcgtgat gagcatcagc gcattcattt cacaacaaga gatctgccta
    11341 gtaaataaaa aataagataa aggtcatcgg attcgattct gtcctatcag atggaaagac
    11401 tagttgatcg aaaaaaaggc tgtaccaaat tttggtgcag cctgttttat ggagcattgc
    11461 ccgctctttt ttataacaat tttttacaga aaatggtact attgaagtat taataagtga
    11521 aaaacagatg agtaagagga ggcaagtagt atgaaagagc cagatacaaa tgcaaattat
    11581 gagccagacg aactagagga gacgaatgcg gatcaggata caggacaaga atcgtcttct
    11641 ttttacgata tcaaagaaaa agcaagcatg aatctttccc aattattaga agacttacag
    11701 gcttttgaaa aagctgtccg cgaagaagat atccctgaga tctaccgttt atacaatggc
    11761 cgattgaatg aagaattaaa aagaagctca aatgaaaatc acgagatcga tcaattgctg
    11821 atgcgtaaga tccatgatcg ttttttacag gaatttcctt ttatggaaca agtagaaaac
    11881 atctcgccaa cgatgagcta ttacaaaatc ggcacttatt accatgatcg gcccacggtc
    11941 ggtattgatg caagtctgcc agaaattttt gttttgccta agatcgatga agagtgggag
    12001 aaatattccc aaccggagtc aacggactat gacaaaaaaa tcaatgagct agacgcaaag
    12061 gtgatcactg ctcagacaga aattgaacgt ttagatgaac aaataaaaga aatcaatcga
    12121 caaatgactg atttgaacga caataaagga ttcttgaatc gtaaaaaaat cgaggaagaa
    12181 attcaagaat tagagaagaa gaagcaagta ctatctaatg aaaaattagg ctggcttcct
    12241 tatattgaga cgcctgaaac aatccagcag caaaaagaag cattgaagca agaagcaaga
    12301 gcagaccagc ttcgtgcagc catcgtagaa aaagaacaaa ggcaaatcag acgctatttc
    12361 ggtagcaaag agggattcgg tcaagcaatc catgaattct tgatgaatta tcttggcaat
    12421 gagaatccta ataatcaatc taatgaagga ggttccgagt atgaatgaac acgatgaagc
    12481 agaatggaca aaaaatgaag aacaaacaga agatcatgaa gaaacgaaca cttcattgga
    12541 agaacaaaca agtaatcctc tggatgacac agaagaaatc cctatgctag aggaaacaaa
    12601 aatacacgaa aaagaaaaag taactccgac gaatacgaat actgcagaaa acacccatgc
    12661 ttacgaaata actgaaaatc ccgggtattt agaaggaaaa gagcaagatc atcaagatat
    12721 cgtcagtttg attgaaacag cggaagcaga attagcggat ttacgtatga gaaaagattt
    12781 gatcgaatca gattttccac aattattaga atcatatgat cgttttatat tgaatgaaga
    12841 accaatctcc acgatcggaa agacaagcct gatcgaatac ttcacgtatg aattactgaa
    12901 accgttgaat gatattggtc tggaacggtc aaattcagct tatgacacat ggaaaacaag
    12961 acatttttcg atcaattata cgttgaatgg acaaaacgaa ttagtctttt cctttgtatt
    13021 accgaccatc gatcaacgat accaagtgga atcgatgagt ctcctaaacg tatcaccaga
    13081 gacaatggaa atcaatgtcc aagatgaccg ggtgctttct ttgatccgac actggtcagt
    13141 ggatcgagta ttctctgcag ggcagatcac gatattcaat cacaaattga atcaattatt
    13201 agcccatgca agaacacttg gttttaccgt taatcagaca ttactagaca atacgaagcc
    13261 acttcgtttg aatcttcaga gcgaatttga attgacgaat gaagtattgg atgatatttt
    13321 tatcgtggca atgaaaaatc catcctacga tatggaaaag atcaacgaaa ctacttatca
    13381 agtacgatta gataaaggac agtctttaac gataacaaca aacgaaaaag atcaaacagt
    13441 cttgtcgatc tcatctggag actacccacg atctgtcatt gatttcttta tcaattacga
    13501 attcttggtc ccattaatgg tgagaaagtc ctgattggat aagttggatt accagatgtc
    13561 attcagataa ttctgttcca aaggacttgc ggcagttatc cagaataatt gtcacaagtc
    13621 ttttctgtat gtactaaatg gacgtgaata acttgctgat gaaggaggga tttcattagg
    13681 aatgtccaca aataaaatag cacagcttag cttattgtct gctgcttgtg tagcaggtag
    13741 gatcgctttt caatttattc cgaatttcca gccaatgact gctatttttt tatttatagt
    13801 actttactta agcttaaaag atgcgttaat tgtcatgagt ctttctattg cgatcagcag
    13861 tttttatttt ggtgttggac catgggtcat cggccaatgg atcagctata ctgttgttct
    13921 gagtttgttt tcaatcgtct gcttgagaaa aacagtgcaa aggaatctat ggttaaaggg
    13981 agtctgtttt tttctggcgg gcatggttta cggaataggt atgaccgttt ttgatacgct
    14041 gttatatcgt ttgcctcaac cttggatcta ttaccttcaa ggggtgtcgt ttgatttgat
    14101 gcacagtata ggcaatgttg tgttctttct tgtttttctg ccagtcgtca aacgttttta
    14161 caatcattca ggaggaaaaa atgaaaaaaa taatttatag tttgcttgca atgagtgtca
    14221 gtatactcat tttgacaagt tgcagtacaa cctatacaca atcggcttcc aaccaaaagg
    14281 aggaagtaaa aacatcgcaa aaagcaacaa tcacgttaca agaaaatggt caaaactttt
    14341 cggaaaaaga agtgttcttc aaaaaaggcg atgatttact gagtattttg aaacgaaatt
    14401 ttgaagtcaa agaagataac ggcttcatca cttcgcttga agggcactca caggatgaga
    14461 aaaatcaact ctattggatg tttactgtgg acgggaaaag tgctactact ggtgccaaag
    14521 agatcaaatt gaatgatgga caatcggtca ttttcaattt gtctaaacta taagtttcat
    14581 aaaaaactgt taaagggcga atattgccat aattcagtga gttcagttga tgaaagaagg
    14641 aaaataacat ggaaaatttt aaaaaaagcg gaatgttttc cgtaattgct gctctagggg
    14701 ctaatattct agttgctatc agcaaatttg tcggctatgc aatcagtggc agtgccgcca
    14761 tgctaaacga aagcatccac agtatcgttg attgcagcaa tcagattttg ctgttattcg
    14821 gtgataaaca agccaataaa ggacagagcg agttgcatca atttggtgaa ggacgagcaa
    14881 aatacttttt cagtacaatc gtcgcaatga tgcttttctt cggcggtggg gcacttgggg
    14941 tgatggaagc ttttgaaaag ttgacgcatc catcccatga agtgggtaat ccgatcattg
    15001 ttatagcaat actgttattt ggtttggtaa tcgaaggaag ttcgttacgc gtggcaatga
    15061 aagaaatcaa agaattgaat actgaaaaac tttccacgat gcgctttttg cgcgaaagtc
    15121 gacacagtga aatactgatt atttttacgg aagacttgtg tgctgtaatc ggtttggtct
    15181 tggctttagc aggaacaata ttgacttatg caaccgggat tgctgcattt gatgcgatca
    15241 gcggattgct gatcggtttc ttgctgatgg gagccgccat tttcctagca aaagaatttt
    15301 atagtctgat catcggtgaa agcgtgacag caagcgattt atcaaaaatc aagttagctt
    15361 ttgaacgtcc tgagatcagt cggctgatcg atataaaaac agttcattta agtccaacgg
    15421 agattttagt tgcagcaaaa gcagatatcg ttggtaacaa agagaatgaa gcttactcgg
    15481 tcatcaatga tattgaaaaa aatatgcgtc agtctcttcc tgacaagaag atgtatatat
    15541 acattgaaac agataaatat gacccaaatt attctcgtaa ataaaacgtt tattcatgaa
    15601 ttgaagcaaa aatgagaaaa atataatctt gtttttaaat aaaacggcta caatttagcg
    15661 aatttatgtt acaatgagtc cgctgtttat ttgttgtaat taaaggagat attttaatga
    15721 acaaaaagaa aagtgcgcca tggcttcaag ctttgcatgc agtgatgcca ctttgtataa
    15781 gctatatacc agtaggatta gcttgcggag tattgttgca aaaagttgga tttgatcctc
    15841 tcttatcagg actgttatcg attctggtct tttccggtgg cgcgcagttt ttagttgcat
    15901 ctatgcttac gacgcaagca tcatttgcaa cgacattatt gatggttttc tttttagaat
    15961 tacgctatac attattagga gcaagtcttg ccggttttat gaaaaaagaa aagcggacgt
    16021 ttttggctgt cttttctcaa tcactgaatg atgaaaacta tgcggtgaac tacttaaagt
    16081 tttcaactga cccttcctgg aataaaagaa aggctttata tgtcaattgg ttctcaatga
    16141 ctgcatgggc cggtagcaat atgatcggta attttttcgg ctcggtgatt cgagtagatg
    16201 ctgaccttgt ccattttgct ttgacggcca tgttcatttt catgtttatc atgcagatga
    16261 aaagcgcact actgatcttt acaggtttat tctcaggcgt attaggggtc gtattcatgg
    16321 tattattcca aaatactttc ggattgattg cagcaacagt cattgcttca tttgccggat
    16381 ttatgctgga gaaggcattc aaaaaagaaa aagctaagaa actaagagac cgcggtaaag
    16441 atgtcaatga gccgctcttc aacttcccgg aacaggaggc caatcaccat gactaaacta
    16501 tatttgctac ttttagttgc ttgtctgttc atcgtggcat atatcccgcg cttgttcccg
    16561 atgctttatt ttactcatcg aaaagtgcca tcctggttca gtgactggat gaaatatgta
    16621 ccggtggcat tatttgcggc gttagctttt aaagatgttt tcattactca tgaacatctg
    16681 gatattgctt ggaatatcaa aatagctgca atgattctag tagcaggcgt ggcttacaaa
    16741 acacgttcga tggcgttgtc agttttgaca ggacttgcgt ctgtcttttt gctgtctatg
    16801 ctttaaactg ttttatttta ttaaaaacag agattgcgac ataagttgtt caaactgatc
    16861 tatccgaact gcatttgcaa cagtcaattc tggatgcaag tttattcgta gggataaaaa
    16921 agattagtgt tggcttatga tgcaagctct tttttatttt aggaattaga gagggatttg
    16981 tccttctatg attttttgct ataataggga agatgtttat tttatgaaag aatagttgag
    17041 ggaatttaaa tgaataaaaa attgatgcaa ggaacatttt ggctaacctt tgctaatctt
    17101 ctatgtaaag ttttaggggt agtctatttg attccctggc taagtatgat ggggaacaat
    17161 caagatggta tgcttgctac gacattgtat aatgttggct atcttcctta tggattgttc
    17221 ctgatgcttg gtacagtcgg atttccaaat gcgattgcaa aaaaagtagc tgtagccaca
    17281 aaagaaggag ataacgctgc ttgtcggctg atttttagaa gtacgatcaa tatcatgttt
    17341 gtcatcggta tcgcctctgc tgcactgatg tatttatttg ctcctttact agccggaatc
    17401 agtccgattg ctaacgttaa taatgggata ctagctatca gaagtttatg cccatctttg
    17461 attgctatcc caattcttag tgcaatgaga ggatatttcc aaggaaaaaa tgatctgcgg
    17521 ccttatggaa cttctttgat tatcgaacag gctgtgcgag tgatcgttat cttggctggt
    17581 acttattact tgcgcgtatt gactgacgga accatattag aagctgtatt gatcagtaca
    17641 gtcgcttctt tcttcggtgg gttagctgcg attatccata tgtatatcgt cggacgcaga
    17701 aaagacttct tcgaacttaa agattttttg atttccagcc gttatttcga aagagaaaac
    17761 cgatctgcct ctgtctctat cattcgggaa acactaccgt ttatttttgt cggctcagtc
    17821 attactatcg ttcaaatgat cgaccaagta acgatgaaac cactgcttca ctttttccgt
    17881 ccagagatcg cagatcagca actggaattc ttattcagcc gtgcttctgt caatccaaat
    17941 aaattgacat tgatcctgat ctccatggta ggaacagtag ctataagcag cttgccaata
    18001 ttaagcacga tgaagaaaaa agatcggata caaatcgaaa aaacagtagg agatagtttt
    18061 tcgattgcgt tattgattct tcttccttca ttgactggaa tgtctttatt agctggacca
    18121 ctatacacat tgttttttgg ctatgatcct gaaagtgtcg gatatttcca aatggctttg
    18181 ctagcttctt tgtttttctc tttattcacg atcctatcta cgatgcttca atctttgaat
    18241 caccatcgtt ttgcgatccg attaactgta gaagcaatta tcctgaaagt catcttccaa
    18301 gtaatcggat taggattagt tggcggatac gggatgagct tatcaaatgg cttagcattc
    18361 ggtattgtct ttgttcgcgg ctatcacttt atgtgcaaag aataccgaat agcaccttta
    18421 gcaaagatca gcaatttttt cttaaaaact ttccgctcca ccctgattat gcttgcagta
    18481 tgttttgccg ttttcttttt gctgaatcag cgattgtcaa tggaatcaaa aagctatgca
    18541 atgatttatt gtgtggctgt aggaagtatt ggcggcttag tatttttatt ttctcaattc
    18601 ggaaaaaatg ggtttcgaat gttgaaaaat gttaaacatc atagcagaag caaagaatag
    18661 gttaactaat cgttttttgc tgtcacttga aaaacggcaa ctcgtatgat tacgcttgtc
    18721 gtttttctat tttccaattt tagtttcata tctaatctta cttatgatat agttataagg
    18781 tctggaaaaa ttctttgaaa cgaggaaatt aaacacatga aacttgcagt agttacagat
    18841 agttcggcat atctgccgga acgtatcaag aaccatcccg acttattcgt tattccaatc
    18901 cctttgatta tggatgggaa aatttataac gaaggaatcg atatcgaagc ggatgaatat
    18961 tatggacttt taaaaaacag taaagatttt cctactactt cacaacctgt cgtaggggaa
    19021 gttttgggtt tatatgagga attgaaagag aaagggtatg acacgatttt aagtatccat
    19081 ctttcttcag ggatttctgg ctttgtcaac acattatttg cgatgaaaga tgacatcagc
    19141 ggagtaacgg tcattcctta tgactctaag atcactagta tgccgatggg acacatggta
    19201 gaagctgcct tagacttaaa tgatgaaggt cagtcccttg atgaaatact tgcacatatc
    19261 gatcgaatcc gagataatac ctatgcttat ctgattgtag atgatttgaa caatttagta
    19321 cgaggaggac gtttgacaaa tggtgcagcg ctgattggca gcttattaaa aatcaaaccg
    19381 atcctgacat tttctgaagg gaaaattgtt ttattcgaaa aaatccgttc aagtaaaaaa
    19441 gcatttgctc gagctgaaga aattattggt agaagaaata acgaaataca accgccagtc
    19501 aaactttacg tgatacatgc aaataattta gaagtagcga ttgcggagaa agagaaattg
    19561 cagaaaaaat atccaaatgc agaaatcgaa attggtcatt tcggaccagt cattggtaca
    19621 catttaggtg aaaaagccat cggcattgcc atttcagcgc aataattcct tttattcaaa
    19681 aaacatacca aaaat
//
LOCUS       DAHHZJ010000039        18504 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.040, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000039 DAHHZJ010000000
VERSION     DAHHZJ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.040"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            99..746
                     /locus_tag="NBL02_001129"
     CDS             99..746
                     /locus_tag="NBL02_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HCD2571829.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="NBL02_001130"
     CDS             1139..3784
                     /locus_tag="NBL02_001130"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCD2571830.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FYRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="NBL02_001131"
     CDS             4094..5407
                     /locus_tag="NBL02_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCD2571831.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="NBL02_001132"
     CDS             5397..6050
                     /locus_tag="NBL02_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCD2571832.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6797
                     /gene="radC"
                     /locus_tag="NBL02_001133"
     CDS             6105..6797
                     /gene="radC"
                     /locus_tag="NBL02_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCD2571833.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6947..7147
                     /locus_tag="NBL02_001134"
     CDS             6947..7147
                     /locus_tag="NBL02_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCD2571834.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7401..9080)
                     /locus_tag="NBL02_001135"
     CDS             complement(7401..9080)
                     /locus_tag="NBL02_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCD2571835.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9082..9294)
                     /locus_tag="NBL02_001136"
     CDS             complement(9082..9294)
                     /locus_tag="NBL02_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HCD2571836.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9687..11417
                     /gene="efrA"
                     /locus_tag="NBL02_001137"
     CDS             9687..11417
                     /gene="efrA"
                     /locus_tag="NBL02_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HCD2571837.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11414..13174
                     /gene="efrB"
                     /locus_tag="NBL02_001138"
     CDS             11414..13174
                     /gene="efrB"
                     /locus_tag="NBL02_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HCD2571838.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13269..13466
                     /locus_tag="NBL02_001139"
     CDS             13269..13466
                     /locus_tag="NBL02_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571839.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            13619..14104
                     /locus_tag="NBL02_001140"
     CDS             13619..14104
                     /locus_tag="NBL02_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCD2571840.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="NBL02_001141"
     CDS             complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="NBL02_001141"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCD2571841.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            14981..15508
                     /locus_tag="NBL02_001142"
     CDS             14981..15508
                     /locus_tag="NBL02_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571842.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            15599..16336
                     /locus_tag="NBL02_001143"
     CDS             15599..16336
                     /locus_tag="NBL02_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCD2571843.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            16340..17257
                     /gene="miaA"
                     /locus_tag="NBL02_001144"
     CDS             16340..17257
                     /gene="miaA"
                     /locus_tag="NBL02_001144"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCD2571844.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            17259..18488
                     /gene="hflX"
                     /locus_tag="NBL02_001145"
     CDS             17259..18488
                     /gene="hflX"
                     /locus_tag="NBL02_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCD2571845.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         5984 a         3200 c         4021 g         5299 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta ttttatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
     6661 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
     6721 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
     6781 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
     6841 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
     6901 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
     6961 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
     7021 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
     7081 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
     7141 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
     7201 gcgagtaaca aaaatacctg ccacaagaga caatttctct tgtggcaggt atttttttgt
     7261 atgaaaaagt aaataaattt aagaggctat gaaagagcca ccttgtatca aggaataaga
     7321 acaaacaaaa atggcttctc atgtttttca tttgttttct cttatattca ggacaaggtg
     7381 ctgatttcat aacctcttat ttattcttca tctggtgtca ttaccattgg caaaatcatt
     7441 ggatgtcttt ctgtacgttc gaataagaaa ggctgaagcg cgccagtcat cgcttctttc
     7501 aatttatatt ctgaacagtc aggtccattc aaacattctc ttaaagcgtg gaataagatt
     7561 cgctgagctt cattgatcat ttctcctgat tctcgcatgt agacaaatcc acgagagaga
     7621 atatctggac cagccaagat ttcttttttc ttgatatcca ctgtcgcgac agctaataca
     7681 agaccttctt ctgaaagcag gtgacggtca cgtaaaacga cattaccaat atcaccgatc
     7741 ccgctaccat cgatatacac atctccagcc acaaaatgcc cagcaggacg cgcagaatcg
     7801 ctagttaacg ctaaaacatc gccattttcc atgatgaagc agttttctgc aggaacacct
     7861 acatcttgtg ctagagaagc atgtactttc aacatgcgga attctccgtg gacaggaata
     7921 aaatattttg gcttcatcaa acgaagcatt aatttttgtt cttcttgtcc accgtgaccg
     7981 gaagtatgga tgttattgat ttttccgtga atcacttctg caccagcttc agacagcaaa
     8041 ttgatcaaat gattgacact tgttgtatta ccaggaatag gtgaacttga aaagatcact
     8101 gtatcgtctg gctggatttg gatttgacgg tgagtaccat tcgcgatgcg gctcaacgcg
     8161 gccatcgctt ctccttgaga acctgtacat aggatcatcg tttcattggc aggcaagcga
     8221 ttgatttcgt gtgcatctac aaatgtgccg tctggaactt tgatatatcc taaacgttga
     8281 ccgttgacga tggcattttc catgcttcgt ccgaaaacag cgattttacg accagttgcc
     8341 acagcggcat ctgctgcttg ctgcaaacgg aagatgttag aagcaaagct ggcaaaaatg
     8401 atacgtcctt cgatttttga aaagatcttc aaaatagaat gcccaatcgt tttttctgat
     8461 ttagtaaatg ttggaatctc agcatttgta ctatcagata aaaggcacaa gactccttct
     8521 tccccgatac gagccatttt atgcaagttt gctggttcgc caacaggagt gaaatcgaat
     8581 ttgaagtctc cagtcgctac gatatttcca gaaggtgttt tcaccacgat ccctaatgca
     8641 tctgggatac tatgggtcgt tctgaaaaag ctgacagaag tctttctaaa acggatgatc
     8701 gtatcttcat tgatctcatg gagctctgca tctcgaagca agccatgctc atctaatttg
     8761 tttgtgatca acgctaatgc gagaggacca gcatagatag gaatatttgc ttgtttcagc
     8821 aagtagggta ttccgccgat gtggtcttcg tgcccgtgag taatcaccaa ggcttttact
     8881 ttgtgtaagt tttgtatgat gtagctgtag tcgggaatga catagtcaat tccaagtaaa
     8941 tcgtcttccg ggaatttgat cccggcatca atcaaaatga tttcatcttg gaattgtacc
     9001 ccatacgtat ttttcccgat ttcacctaat ccaccaatag caaagacgcc tgtctcgtta
     9061 tttttgatgg aaagtttcat attaaaactc cactaatgag aaatcagcat tttctttttc
     9121 gtactctaga tgacttcctt ctagaagttg aacgaattca atattgtatg gggtattttc
     9181 ttccacacgt tggcgagcgt ctacatcgct ttttgcctca atgtaaagag attttgtttc
     9241 ttcccgttta gggttgcgta cttttgtttc ttgataataa actttataga tcattgtttt
     9301 gtctccttta tctgcgtaaa gcagtgattt agctagttga tttttttaga caacacaaaa
     9361 cgaggggaca agccacctgg tttttcatat gctttgacta gaatactgga ttcgagtcaa
     9421 agagacaagc ctcgtttttg cttatctgtt cggatatttt ttcaaccgta ccttctgtat
     9481 aaaaaatata caacaattaa ttgttattct agcataaaac agcaaataag tatagaaatc
     9541 aactagattg aaagaaaaaa tcaatgttta tcatcccgct ataatcataa tgactttttt
     9601 atgagagaag tgttctatta ataggctcgt tgtgctatca tttttagtaa tgaaaatgta
     9661 acaaaagatt aaaaggagat gagaaagtga aactaatgtg gcattatacc atgcgttata
     9721 agaaattctt gtttttcaat ttcatatgcg tatttggttt tattttgatt gaattaggac
     9781 ttcctacgat tttagcacgt atgatcgatg taggaatccg caatgatgat tttgattatg
     9841 tgaagcaaca agggatattt atgatcgtta ttactgtaat cggtattatt ttgaatatct
     9901 tgttaggtta ttttggttcg aggattacga ctaatatcgt agcagatatt cgagatgatt
     9961 tgttcgaaaa gatccaaagt tattcgcatc aggaatacga aaatctaggc gtatcttcat
    10021 tgatcactcg tacaacaaac gatgcttatc agatcatgtt gttcctgcaa aatattttac
    10081 gtatcggttt tatggcacca atgatgttca ttgtcagttt gtacatggtc atgcgtacaa
    10141 gtgtgacact cagtttgtat gtagtaggtg cattaccttt actattattg gcagtcgtag
    10201 cgattgctaa ggtgtctgaa ccgttatcta aaaagcaaca aaaaaactta gataaaatca
    10261 acagtatctt gcgggaaaat ctttcaggat tgcgtgtgat tcgtgcattc gtcaatgaaa
    10321 agtttgaaga aaaacgtttt gctgtagtca atgagaatta tgcttctagt tcaaaaagct
    10381 tattccgttt gatggctgtt gcacaaccgg gtttttattt cttatttaat atcgtaatgg
    10441 tattgatcat ctggagcggg gcagtgcaaa tcgatgcagg cgagcttcgt gtaggagatt
    10501 tgattgcgtt tatcgagtat atattccatg ccttgttctc attcatgtta tttgctagtg
    10561 tgttcatgat gtatccaaga gcagctgttt cagctggacg tatccaagaa gccctagatg
    10621 ccaaaccttc gattgtagaa gatccaaatg gtatatctga tacaaaaact aaagggtata
    10681 tcgaatttca aaatgtaaca tttgcttatc caggccattc gcaagagcca gtgatacgaa
    10741 atgtcagttt tacagcctct ccaggagaga ccgttgcttt tattggaagt accggtagcg
    10801 gaaaatctac gttgatccaa ttgatccctc gcttttatga tgtgagtgaa ggaaagatct
    10861 tgatcgatgg agtagacgtg agagattatc ggctaagcaa gttgcgagat aagattgggt
    10921 ttattccgca aaaagcgctg ctatttactg gaacgattgc agataatttg cgatacggga
    10981 aagaagatgc tacgcaagaa gaaatggaac gtgcagctga gattgctcaa gcttcagaat
    11041 tcatttctcg aaaaccagat ggatttaatg aacatttatc agaaggtggt tccaattttt
    11101 caggtggaca aaaacaacga ttggcaatag ccagagccat catacgccgt ccggaaatct
    11161 atatttttga tgacagcttt tctgcattag attatcaaac ggatgcgaag ttacgtgctc
    11221 gtctgaaaaa agaaacgaca gaatctactg tattgatcgt agcacaacgt gtgggaacga
    11281 tcatgcatgc agataagatc atcgtattaa atgaaggaga agtcgtcggt atggggacgc
    11341 atcgggagtt gttggaaaaa tgcccgattt attatgatat tgccgcttct caattatcaa
    11401 aggaggaatt agcatgaaaa aaaccttttc ttccttaaaa cgtttagctc gatatatcag
    11461 accgtatcgt gagacttttc ttttagtgat cctatttaca gttctgacag tggctttcaa
    11521 cgtggctttg ccatatgtta caggacttcc tactacagaa atcagccgta atattgccaa
    11581 tggcgaatca atcaattttt cttatatatt caaatgtctc atttggatca cagttgttgg
    11641 aatagggtat tgtatttcgc aattactttc tggtgttttg atgacaaatg tcgttcaaag
    11701 tgccatgcat gatttgagaa gagacataga agaaaaaatc aatcgtcttc cggtttcata
    11761 tttcgataaa aaccagcaag gaaatatttt gtctcgtgtg acgaatgatg tagatgcagt
    11821 cagcggtgct ttacaacaag cttttattgg tatcgtaaat gcgatattgg gaattacgtt
    11881 ggctgcagca atgatgtttt atatccagcc gatgatggcg ctgatctcta tgatcatgat
    11941 tccaacatcg atctggatct caaaacgagt gatcaatgct tcgcagaaat attttcaaag
    12001 catgcagaat tctcttggtg agctaaacgg ctacgtccaa gaaaatatga caggttttag
    12061 tgtattgaaa gtatatggac gcgaaaaaga aacattggaa ggattcaaaa aagttaatca
    12121 cagcttgaaa tactttgggt ttcgtgcagc attcatttct ggcttgatga tgcctctagt
    12181 tcaattaact gcctatgcta cttatattgg tatggctgtt ctaggaagct tttatgttat
    12241 tactggggcg atcgttgtcg gtcaactaca agcattcatc caatatattt ggcaaatcag
    12301 tcagccaatg gggaacgtga cacaattgtc ttcagttttg caaagtgcat cagcggcaac
    12361 gaaacgtgta ttcgagattt tagatgagcc agaagaaaaa gaaaacaaag tagatgcttc
    12421 actgccagaa acgattttag gagatgtgac attcgatcat gtcgattttg catatgatcc
    12481 gaagaaacca ttaatccaag atttgaattt tgaagtgaaa gctggtcaaa ccgtagctgt
    12541 ggtcggtccg accggtgccg gcaagacgac attgatcaat ctattgatgc gtttttatga
    12601 cgtagacaaa ggagcaataa ggattgatgg aatcgacacc aaatcaatga gtcgcagtga
    12661 tgtgcgttcg ctatttggta tggtcttgca agatgcatgg ctttatcaag gaacgattgc
    12721 agataatatc cgcttcggaa agcttgatgc gacagattat gaagtagttg atgctgcgaa
    12781 aacagcaaac gtagatcatt ttatccgaac gatgccagat ggatatgata tggccatcaa
    12841 ttctgaggga gacaatgtgt ctttaggaca aaaacagttg ctgacgattg cccgagcagt
    12901 ggtctccgat cctaaaatct tgattttaga tgaagcaaca agttcagtag acacccgttt
    12961 agaagcgttg attcaaaaag caatggatcg cgtgatggag ggacggacaa gtttcgtcat
    13021 cgctcaccgt ttatcgacaa tccgtgatgc agatctgatt ctagtgatgg atcaaggtca
    13081 gatcattgaa aaaggaacgc atgagagttt attggcacaa ggtggatttt atgaaaaact
    13141 gtataacagc caatttgctg aggaagcaga ataagtcata taagaataaa acagtttaaa
    13201 acttattttt tgttctctat tcttgataat ggtaaagtaa agttatcaag aaaagtgagg
    13261 acacggagat ggaacagttt ttaaatgaaa aaattactga agccaaaaga gaagttgatc
    13321 gtttaacgca cgtctgtcag acgaagcctt ttgattcagc tgattttttt gaaaatgagt
    13381 ttcaaatcga acgcttggca gcacaaatag aagtgtacga attaatttta aaaaaagtaa
    13441 tagaagaaaa tatagatgta caataaaaac aggtaaaacg tcagttacca gatactgtaa
    13501 atagacatct ggcctgagca ggttttacct gttttttttc ttatgaaata aatatatgct
    13561 atgatacctt tattcaatag gattagtttc tacaaaaaat gaaaggagaa aaataaatat
    13621 ggaacggttt atagcatcaa atgccacagt gattggtgat gtgacattgt cagaagatgt
    13681 gacgatctgg tatcaagctg ttttacgcgg ggacagtaat tggataaaaa taggacaaag
    13741 aacgaatatc caagatggta caatcatcca tgtcgatcat gatgcacctg tagatattgc
    13801 ggagaatgtg acggtaggac atcagtgcat gctacatggc tgtacaatcg aaaaaggggc
    13861 gctgatcggg atggggacca cgatattgaa tcatgcggtt attggtgaga atagtttgat
    13921 tggtgctgga tcattagtaa cagaaggaaa agttattcct cctaatgtct tggcttttgg
    13981 tcgcccagct aaagtgatcc gtcctttgac aaaagaagaa atacaaaaaa acaaagaaaa
    14041 catccagcat tatgtcgaga taggtcggat gcatgcgcaa ggaaaattca aggagcaaag
    14101 ataggcagta gcatccataa aataaaaaag gatggtcagt ttgagttgac catccttttt
    14161 tatttggttt agataagaga gcttaaaaaa gcttcctttg aaacagatta atttaatgaa
    14221 gaactgtttt ccatgacttc atcgatcaat ccatatgctt ttgcttgttc agctgtcata
    14281 tagttgtcac gatctgtatc tttttcaatc acttcaagcg gttgtccagt gcgttcagct
    14341 aaaattttgt ttaagcgttc gcgtgtttgc aaaatatggc gtgccgcaat ttcgatttca
    14401 gttgcttgcc cttgagcgcc gccaagaggt tgatggatca tgatctcagc atttggtaaa
    14461 gcaaatcgtt taccttttgt accagctgtc agcaagaagc ttcccattga agcagccata
    14521 cccataacaa ttgtttggac atctgctttg acaaagttca ttgtgtcgta aatagccata
    14581 cctgctgtaa ctgaaccacc aggagaattg atataaagat agatatcttt ttcagaatct
    14641 tgagcgtcta agaaaagcaa ttgggcgatg atcgaattag caagatcatc tgttacttgg
    14701 ccgcttaaca tgatgatacg gtctttcaat aaacgtgagt agatatcgta agctctttca
    14761 cctcgtgaag attgttcaat gactgtagga attaaattca taatagagtt cctccttgat
    14821 ttcgtttaga aatagtgtac cataatttag tttctaatac ctaaaatgta gtatacattt
    14881 ttggtcaaaa aaggtcaaat aaaaaactta ggcaaattag tcgcataaac ccgctttatg
    14941 gtatgataag cagagaaaca aaacaaggag gaacaacaga ttgatagttg gtattactgg
    15001 ccttatcgga gtgctgacag gtgctgtagt tgtctatatc ggcatgatac tgcaaatgcg
    15061 tcacttggaa aataaagaac agcgtagacg tgaattggaa ttgaaagtca aagaaatcga
    15121 gacgctgaat caattaaata aaaagatcaa cgagatcttg caaaaacgga caacgctgat
    15181 ggaagaatat gtcagctttg atgcatttga tgattgttat atcacgattg acgattttgc
    15241 gtatctccaa tcattttcag cgcaaaatag tttttatctg ccaaattata ttttagacga
    15301 gctatttaag aatatctccc atcgaaaagt gattttatct ccagatgaaa caatgaagat
    15361 tggtggatat gcttataaag gcggacgcgt gattttagag aattttacgg atagtctgac
    15421 tgaaatgatc aatgaaaaaa aagctcagtt gaagactatg acgcgccagc cgattggttt
    15481 cttctcgaaa gatgatgttt ggagctgata ttttgaagca ttgccgttgc acatgagagc
    15541 gtggtaatgc tttctttttt tcttaatgat acaatagaac tgaacggagt gaagaaagat
    15601 gttacaaatc tatgcccatc gcggaagcag cggcacccac ccagaaaata cgcttcctgc
    15661 atttgcagaa gctgtacgag taggtgcaga tggtatcgaa ctagatgtcc atctaagcaa
    15721 agatggttat ttgatcgtta tgcacgacga agaagtggac cggacgacta acggtaaagg
    15781 gctgattcga gaaaaaacat tggaagaact caaaaaattg aatgcagggg gcaaattcag
    15841 caaagaattt ccagcagcta aagttcctga attgagagaa gtgttaggac tgctcctcca
    15901 aaaaaattac cgagggatgc taatgattga aattaaaact gatcagtatg aatatcccgg
    15961 tatcgaagaa aaattagccg aaacattaaa aagtagagat tggccttttc aacatgcata
    16021 ttgcagcttt catttggatt ctttggaaaa aatcgcatta ctggaaccgg agatacagct
    16081 ggacttattg atgagtactt ctgataaaaa gatccaacta gcagcagaaa ccccttatat
    16141 agaaggagta catccaaaaa atacttggtt gttcgaacat ccagtccagg cagctgattt
    16201 tccaaaagca attcgtgcat ggacgattaa cgatgaagaa gaaatggcca aatgtcaaga
    16261 actcaaaata gatggaataa taactgattt tccggagaaa gctcttcggt ggaaacaatc
    16321 aacgaaaagt aggtaaaata tggaaaaagt attagtgatc gtcggtccga cagcagtcgg
    16381 taaaacagcg ctaagtgtgg aactcgcaca actatttgga ggagagatca tcagtggtga
    16441 ttcattgcaa atctataaaa atctggatat cggtacagca aaaatcaaag aagaagagat
    16501 gaataatgtt ccgcatcatt tgattgatgt gatcgaacct actcaacgtt actcagccgc
    16561 tgattttcag cattcaggtc gacaattgat taccgagatt gcgaaccgag ggcacctacc
    16621 tatcatagct ggtggtacgg gtctctatat ccaatccctt ctatatgatt atcagttagg
    16681 agcgacagaa gaagcgaatc ctgagattag aaaaaagtat gaaacgttag ctgaaacaat
    16741 tggttcccaa gcattatgga atcagttgaa agaaaaagac ccggcagctg ctgagaaaat
    16801 ccactggaac aatcaacgca aagtgataag agctctggaa gttttagaga cgacaggaca
    16861 tagtatcacg gcacctaaag aagagccaaa gcaattgtat gattacctca tgattggatt
    16921 agatacgaag cgtagtattc tttatgaacg aatcaatcaa agagtcgatt tgatgctagc
    16981 agaaggttta gtagcagaag cgcgtaaggt gtatgagctt ggagaagtcc aggccagtca
    17041 aggtattgga tataaagaat ttttccctta ttttgaaggg aaagaaacat tggaagaagc
    17101 aacagaacaa gtcaaactac attcgagaag atacgcgaaa cgtcagttga cgtggtttcg
    17161 taaccgtttg gatgcacagt ggtttgatct tataagtgag ccaaatcaaa aaagtcagat
    17221 tgagcaaaca atcaaaaaat ggctggaggc ggaataaaat ggaagaaaaa gtgatcatcg
    17281 taggggtaga aaccgaagca aaccagcgtt acttttcaga gtcaatggaa gagctagttc
    17341 agctgacgaa taccgcatca ggagaagtcg tattcacaat tacacaaaag agaccacaag
    17401 tggatcggca aacgattatc ggtaaaggga aattacaaga attagttcaa caagcggatg
    17461 cgcatgaagc ggatttgatc atctttaatc atgagctaac tccgagacag agccaactga
    17521 ttaccgatgc tgttggtctt ccggtaattg atcgcgtgca gcttatctta gatatttttg
    17581 cgatgcgtgc tcgttcgaaa gaagggaagc tgcaggttga acttgctcag ttagaatacc
    17641 tgctgcctcg tttagtaggg caagggaaaa cattatctcg tcttggcggt gggatcggaa
    17701 caagagggcc aggtgaaaca aagttagaaa cagaccgccg acatatacgg aataaaattt
    17761 ttgctgttaa gaaagaattg aaagaagtag aagctcatcg agagcgcaat cgccaaaaaa
    17821 gaaaaaatag cgagattttt cagattggac ttattggcta cacgaatgcc gggaagtcaa
    17881 ctatcttgaa tctattgaca caagcggata cttattcgaa agatcaattg tttgcaacgt
    17941 tagatccatt gactaagaga tggcgttttg ctgaaggatt tgagattact gtaacggata
    18001 ccgtcgggtt catccaagac ctgcctacgc aactgatcga tgctttccat tctacattag
    18061 aagaaagtca aaatatggat cttttgctac atgtagtcga tgctagttct ccagatcgta
    18121 ttttacagga gcagactgtc ttaaaattga tggatgaact gaatatgaaa gaaatgccga
    18181 tactgactgt ttacaataag gcagaccaga tcgacccagc gatgttcacg cctacgctat
    18241 tcccaaatgt attgatttct actcagacga aagaaggtaa gatggcgttg atcgaagcaa
    18301 tcaaacgtca gctgatggaa ctaatgaccc cttatacact ttctgtgcca agcaacgaag
    18361 gacagaaatt aagcgagttg agaagacaaa caatggtttt ggacgaagcg tacatggaag
    18421 aaacaaacga atatgaagtt aaagggtttg caaaaaccaa ttcaaaatgg ttaagaaaaa
    18481 accaataaaa ttttattttt ttaa
//
LOCUS       DAHHZJ010000040        18055 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.041, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000040 DAHHZJ010000000
VERSION     DAHHZJ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.041"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            214..498
                     /locus_tag="NBL02_001146"
     CDS             214..498
                     /locus_tag="NBL02_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HCD2571846.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(779..1198)
                     /locus_tag="NBL02_001147"
     CDS             complement(779..1198)
                     /locus_tag="NBL02_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HCD2571847.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(1200..1784)
                     /locus_tag="NBL02_001148"
     CDS             complement(1200..1784)
                     /locus_tag="NBL02_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCD2571848.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1831..3306)
                     /locus_tag="NBL02_001149"
     CDS             complement(1831..3306)
                     /locus_tag="NBL02_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HCD2571849.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPTMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3487..3798
                     /locus_tag="NBL02_001150"
     CDS             3487..3798
                     /locus_tag="NBL02_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571850.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3913..6249)
                     /locus_tag="NBL02_001151"
     CDS             complement(3913..6249)
                     /locus_tag="NBL02_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD2571851.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6391..7059
                     /locus_tag="NBL02_001152"
     CDS             6391..7059
                     /locus_tag="NBL02_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD2571852.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7062..8105
                     /locus_tag="NBL02_001153"
     CDS             7062..8105
                     /locus_tag="NBL02_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCD2571853.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8342..9223)
                     /locus_tag="NBL02_001154"
     CDS             complement(8342..9223)
                     /locus_tag="NBL02_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCD2571854.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9382..10110
                     /locus_tag="NBL02_001155"
     CDS             9382..10110
                     /locus_tag="NBL02_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCD2571855.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10266..10583
                     /locus_tag="NBL02_001156"
     CDS             10266..10583
                     /locus_tag="NBL02_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCD2571856.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10576..11184
                     /locus_tag="NBL02_001157"
     CDS             10576..11184
                     /locus_tag="NBL02_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HCD2571857.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11239..12030
                     /locus_tag="NBL02_001158"
     CDS             11239..12030
                     /locus_tag="NBL02_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HCD2571858.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            12168..12656
                     /locus_tag="NBL02_001159"
     CDS             12168..12656
                     /locus_tag="NBL02_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HCD2571859.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12707..13579
                     /locus_tag="NBL02_001160"
     CDS             12707..13579
                     /locus_tag="NBL02_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HCD2571860.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13755..14117
                     /locus_tag="NBL02_001161"
     CDS             13755..14117
                     /locus_tag="NBL02_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCD2571861.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14126..14878
                     /locus_tag="NBL02_001162"
     CDS             14126..14878
                     /locus_tag="NBL02_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2571862.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14892..16694
                     /locus_tag="NBL02_001163"
     CDS             14892..16694
                     /locus_tag="NBL02_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2571863.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16971..18011
                     /locus_tag="NBL02_001164"
     CDS             16971..18011
                     /locus_tag="NBL02_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCD2571864.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
BASE COUNT         5664 a         3133 c         3731 g         5527 t
ORIGIN      
        1 aggaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca
       61 gcttctcatg tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt
      121 ttattcgaaa aaaggaactg cgacaagact tttgtcacag ttccttcttt tttttgctat
      181 gctatactag atgtaaaaaa taggagaaat aaaatgaggg aaatttattc ttttgaatca
      241 aaaatctatg cttccgaagt cggcaagggc ggggcatata ttatttttcc gtatgatata
      301 agaaaagaat tcggtgctgg aagagttaaa gtccaagcaa cttttgacaa tgtgccatat
      361 gaaggcagta tcgtgaatat gggcgtgaaa aatccagatg gttcagtctg ctacatcctt
      421 ggactacgaa aagatattcg taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt
      481 acagtgaagc aaaaataaaa gaggttgtga caaaagacgt gtcacaacct cttgataaac
      541 ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca
      601 tgtttacaaa atttttctta ctactcgaaa cgaagcggct ttatcacaac ctcttgataa
      661 acggcgttca atcagctgtc cctcggtatt agctcaaaaa cggcaaaata tgagaagcta
      721 tttttccatt tttttctctc atactcaggt cataacgctg atttcacaac ctcttctttc
      781 ataatggttt ttcaccatct ttataataaa ctggttcttt gatatggaaa tctatttttt
      841 ccaaccaatc taaaatcttg taacctaact cttctgacat cggaaaagag gcgttgattt
      901 gtgcattgga gatatggtca acatggatac cggaagtgat cgtacaactt cccggaacgg
      961 cagttttgat gatcacagct attttttcgg ctaagacatc gtctttatgg aaccgaccat
     1021 cgtggctagg gaaacgtaca gtttgcatct ctgtatcacg agaaaatgta gtgactgttc
     1081 cgatatgtgg tgtatctccg cctgttaaag tgatcaataa gtctttaccg attactttaa
     1141 cagcagcttg gatcgtgaaa cctagtttgg ttacttcaac tatcgtttct ttttccatat
     1201 taatcaccct gccaacgttt tgaatgctca tttacaattt caaattgatc caaaagtttc
     1261 attgtatggt gattaacaag atcttggatc gtttgaggct gattataaaa agcaggaatt
     1321 ggtggtatga tttgcacacc tagtcgtgct aatttagtca agttttccaa gtgaatggca
     1381 tttaatggcg tttcacgagg aacaatgact aactttcgtt gctccttcat tgttacatca
     1441 gcagcacgtg caatcaggtt gtcgcctaaa ccaacagaga tacctgcgac tgttttcata
     1501 cttgccggta cgatgatcat tccatcggtc aaaaatgatc cgctggcgac agcagcacct
     1561 aaatctttgt ttgaataatg gtaatcaaac aggctttcga attcagttag tgataagtcg
     1621 gattccagct ttaagttctg ttttgcccat ggactgatga ttccgtgtgt ttctatcatc
     1681 ggatattctt ttagtttctt cactaggttg atggcataaa tggtgcctga agcgccactt
     1741 acaccaatca cgattttttt cttccgttgt gccaattgtt ccattttccg cactcctttt
     1801 tatgatgtca aaacagttgc agatcattca ttatttcaaa tatttttccc aatcttctac
     1861 ttcttggaat tttgcacgta caaaatgttc tttcatatcg aaaggaactg taccgtcaaa
     1921 gatagttttt gcacttatcc cacggaaacg aatagagcca ggattatatt ctggtaattc
     1981 tgatgggtcc aatggatggt tcctcattcc tgggatcacg atgatatctt tatctccttg
     2041 gaaacgagtg ttgaaagtcc aaactacatc gttcatgtcg aaaatatcaa catcttcatc
     2101 gactaaaatg actgtcttca attctttgaa ggctgataaa gcaagaatag ctgcttgacg
     2161 ctgaatacct tcatccgctt cgttttcttt acggatttgc atgatggtca tcaatttacc
     2221 accacctgca ggaggattgt agacatttac tacttttcct ggaatagctt tatcgaccaa
     2281 ggcaagaata cttgcttctg taggaatacc ggccatagat acgtgttctt cactgggacc
     2341 aatcgttgtt tgcatgattg gattgtcttt tcggtgagta actgctttga ctttcactac
     2401 ttgtacagct ggattagcat ccccagtata gccaggaaat tctggcattg cttttcctga
     2461 gttcgtgttg atatcttctt gcattgtttc atttggcatg atttctgctt caatgatgaa
     2521 ttcagcacga gcaataccca cttcatcgac tgctatacca tcaacgattt gaataggttc
     2581 gttacgcaaa gctcctgcac accatagttc attgtagcca agtggagtgg taggtggttc
     2641 gaaagtcgtg ccaatggcaa ttgctggatc taaaccaata ttgatagtga taggcattgg
     2701 tttattcaag gcttcgaatt gtttttggaa gtgaccaatg tggcgtccgc caggcatgat
     2761 gtacataccg atcgtatctt tatcttctag aaccatacga tggatcgtta catcgctcat
     2821 tgttttttct ggatctgatc ctaagactac acccattgtg atgtatggac cagcatcttc
     2881 aggagtattt gtaggtgcag gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag
     2941 atgaacgact tcttgtgctg gagcgtctgc tttgtctact ttcacaggtt tgattggatt
     3001 ttctacagcg tctttcaaga aacgaccaag tgttcgataa tcatggtgca aaatttcacc
     3061 tacacgttta cgactagcca ttgttccgat gttcacacgt gtgccgggga aacctttgat
     3121 attgttgaaa gtcatagttg gtccttcttg agtcggacgt tttactgtac cgccagcacc
     3181 gatataacgg taaacaccag ataattctgc ttcaggatcg atttcaatat ctgtttcgtg
     3241 atattgacct ggaagtgttt ttagttcttc gatgactttt cttaaatcat acggttgatc
     3301 tgtcatgaga gattcctcct tagttgtctt tacctatcta tagtaaaagg tgaaaaagag
     3361 gaaaacaatt caacttgttc ggtaaagtat tctacaatgg aatggatgcg tttttttgat
     3421 aatagaataa actatgatat acttatcact aaaagaaagc gcatacttag tgttggagga
     3481 aggaaagtga aaaaaatgga ggagtcattt cctaaagcag taaaagtgga aaatatagcg
     3541 aatattttga aagttacatt tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca
     3601 gaagagataa ccgatgcctt gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt
     3661 ctcgcacttt ctcgtaatat gtggattggg actgaagtaa cgattgaagc agatggaaca
     3721 gtattcataa atggaaaaga caggtatacg ccagaagagt tgtggtacaa aggaaagaaa
     3781 agtataccag aattataaaa aataattaaa aaaaacagcg cgaaacaagg aacatattac
     3841 ttcctcgttt cttgctgttt tctactattt cagcgctctt atgactcaaa tctaaatgtg
     3901 gttgtcacaa ccttattctg cacgcaaagc gatagcggcg tcttttttcg ctgccattcg
     3961 tgcaggaaga tgaccgccaa gcatcgtcaa tatagtagag acgatcacta gaataatcgc
     4021 atggacagga tttagttgag caacattttt tagatccgtc atgttgtata agatgctatt
     4081 gatagggaac gtagctagcc atgcaataaa cacacctaat atcccagaag aaatacctaa
     4141 aatacacgtt tctgcgtcaa atacgcgagt gatgtctttt ttacgtgcac ctaaagcttt
     4201 tagtacaccg atttcttttg tccgttcaat cacagatgta taagtaatga tactgatcat
     4261 gatcatactt gttactaaag agatccctgc aaaagcaata aggacataag tgatagcatc
     4321 catcaatccg ccagtcagtt cagtcatcgt tccagctaaa tcagtataaa tgattttatc
     4381 ttcatctgat ttgcctttat tgtaatcatc tagataatcc aagatctttt ctttgtcttc
     4441 aaaattgttc ggataaatca tgatacttga aggcaagctg tctccaccta aataactaag
     4501 caatgtttgt ttggcatttt cgtcaacttt ttcagtagtt aagacattta caccactgtc
     4561 tctttgagct ttgacgattt ctgattcttt gttttcgttg acgatttgag tcgttaattg
     4621 atcgctataa gcgattcctg gagataagag attcattgta gaagaagatt tcacgcgtag
     4681 gatccccgag atcttcaatt catcagaagc attctgatac atcgcatcat aatctgtatt
     4741 agggataaaa ttcccagttg gaagttttgt atagtaagta ttgttgttta ctaatttgaa
     4801 tgttgtacca acgatatcat caaaatcaag tttttcgtct tctttcacat caaatccaag
     4861 attcttcaat gcgttgatat tagtattatt attgccgtct acgatcagca cgacatctgt
     4921 tgctgaggct ggataacttc cggcaagtaa agaataattg tcttttaaaa agttttcttt
     4981 gctagtgtca agctgtgtag ggaaacttga cactcccaca cctgtcattg ctgacatcgt
     5041 actagataaa gaaagagatt ccgtatcagg attttgattc gagaagctga caggctgtac
     5101 tttgccattt acatcacgta aaagattgat tcctgtagta cgggtaaatc cgatattatt
     5161 acttaagtta ggatcgatgt ctgttacgta atcgacgtat gtttgatcaa tcttattcgt
     5221 gtgctgtgct ttatcttctt cgctgacttt tgcagtaatc gttttgctgt ctggataatc
     5281 cgctttgctt gcacccaatc cggcatcgtc acgagtctgg ttagtcgtta ccttagaaat
     5341 agtaatcgga aatttcgcca tcgtttcagc ttgcgtgtta tcgatttgtt tttggaatcc
     5401 agaagaaagg gacagaacga tcgcgattcc gatgatccca atactggaag caaaggaggt
     5461 caagaaagta cgtccttttt ttgttcggat attgttgaag gaaagcttta acgctgtcca
     5521 aaaactcatc tttgttctgc gtaagttaaa ttgatcatct tttggtcgtt cgatgtgagg
     5581 atgggaatct gttaagatct taccatcaga aaattcaatg attcgatctg cgtattgatg
     5641 ggccagttca ggattatgtg tgaccatgat cactaatttt tctttcgata actcttggat
     5701 caattccatg atctggatac ttgtttctgt atctaacgct ccggttggtt catcgcacaa
     5761 aaggatatct gggtcattgg ctagtgctcg agcgatggcc actcgctgca tttgtccacc
     5821 agataactga tttggctttt tatgcatatg atttgtcaag ccgactcggt gcaatgcatc
     5881 ttctgcccgt tttcgttttt cgtctttaga aacaccactt aatgtcatcc caagttcaac
     5941 gttttcaata atgcctagat gaccaatcaa gttgtaactt tgaaaaatga aaccgataga
     6001 gttgtttcgg taggcatccc agtcactgtc cttaaagtct ttagtagact ttccgttgat
     6061 taccatatca ccggaatcat aattatctag tccaccaatc acattcagca tagtcgtttt
     6121 accagaacca ctaggaccta aaatcgctac gaattctttt tgtctgaaag cgacagacac
     6181 accatccaat gctttggtgg tggtttcgcc gactttataa tattttttta tatcttttaa
     6241 ttgaagcata atcacccttc tcccgtattg aaatattcgg tatactaata agtatatcag
     6301 aacaatgatc gtagtatacg aaataaatgt taactgaaga tgaactacaa gaaaaaatct
     6361 agaaattttt caaaaaacag agggagatca atgaatcgta ttttagtagt agaagatgat
     6421 ccgcaattac agttgcttta ccgttctgtg ttggaacgag cgggcttttc agtcatcacc
     6481 gcattgaatg gagaggatgc gctgaaaaaa ctagaagcga cacgtataga attaatcatt
     6541 acagatatca tgatgccgaa aatggatggc tatgacttgc tagactcatt acgtagcacc
     6601 agatttgaca cacctgttct aatgattacg gcaaaggccg attttgagga taagaaacgc
     6661 gggttcaaat taggagcaga tgattatatg acaaaaccca tcgatgtcaa tgagatgatc
     6721 ctacgtgtgg aagcattgct tcgacgttca aagatcaatt acagcagtag cttgataatc
     6781 ggacaaacaa aattagagct agatgcttat acagtgttac aagatcagca ttcgttgatt
     6841 ctgcctcaaa aagaatttct gcttctgtat aaattactgt cttatcccaa caagatcttc
     6901 actcgccaac agttgatgga tgatatttgg ggattagata cgaatacaga agaacgaacg
     6961 atcgatgtac atatcaaacg tttaaggacc cgttttgaaa agacggaaga ttttcaaatc
     7021 atcacagtac ggggactagg ctataaggcg gttatttaaa gatgaaaaaa aagctatttt
     7081 cacaattatg gatctatttt gctagtattg tcttcatttc gatcttagtg acactgctgt
     7141 gttttctcgg ttttattttc ttattgtctc gacattctat ctcgcctgct gaatcggctg
     7201 gaaaaccaac gttatacccg ttattcgtct ttgcggggtt cagtatggtc gtgggtacag
     7261 gaatatcgat ttttgttggg cggcgtatcc tgcatccaat cagtgcgcta gggacaaata
     7321 tgagtctggt agcaacggga gatttttcga ttcgaatgga tgaacagcag aaagtagcag
     7381 aagtacagca actgtataaa gatttcaacg ttatggtcca agaactcaat agtatcgaga
     7441 cactgaggaa tgactttgtg tccagtgtat cccatgaatt caaaacacca ctagcaacaa
     7501 tccaaggata tgttcaattg ttacaagccc caaatctctc tgacgaagaa cgccaaatat
     7561 ttttatatcg gattatcgaa agtatcacgc agctttcaca attaacagag aacatcttaa
     7621 aattgaacaa actggaaaac cagcgtatcc aactggaaaa gaaagagtat cgattagatg
     7681 aacaaatcag agaagtaatc gttttttttc agcctaaatg ggagaaagaa cagctagaat
     7741 tggatatcga acttgctgca gtaaattaca caggcaatga agagtttttg taccaagtgt
     7801 ggctgaatat catggataac gccataaagt acaatcaaat caatggacag attcatatca
     7861 aactatttga gacagccaca gagattgtac tggaagtgac cgattcaggt gtaggaatga
     7921 atgaagagac cagagatcga atgtttgaaa aattttatca aggagataca agtcgtcaaa
     7981 tttctgggaa tgggctagga ctttcactgg ttaaaaaaat tctagaactg catgatggac
     8041 gtattgatta tagcagtatc gaaggagtag gaacgacagc aatgatacgg ttaagtaaac
     8101 aataggaaaa agaataaaac ccctaaaaat tctattgaag gtttttaggg gtttttagtc
     8161 gtttttttgt gattagggaa atgttcttac ggttcttttg gcataaaaat cgctgctttc
     8221 ttattgaact acgagaaatg ttagaggtgt cattgaaaca aggacatgat gcaagaaaga
     8281 gagcgaaagt catgcgtcgc tctctttttt tgggtggata gatggacaga aaatgtaggg
     8341 cttagttttc ttcttgattt aaatactccc aaaatgtatc tgaagccaaa gaatgctcac
     8401 cgactttacg agtaaatgca agataactct ctgttgttgg agaaataggg agaatcacag
     8461 tattttcttt taatagattc ttgactgttt tttccataac aatagcaatc cccatgtcat
     8521 ttgcaatcat attgatgatc aagtcgattc ttgctccttt ataagagatt tttggttcaa
     8581 atccagcttc gcgacacaga tctagtacag gttgatacaa gttcgtagtg gggccaagga
     8641 gcaaaaagtt ctcctttttc agttcgctta gatccaattt ctttttacta gctaaaggat
     8701 gatttttagg tagaactgct acaaaatcgt ctgtttcagt aggcaaccac tcaatcgaac
     8761 ttggtggggc ctggaaactt ctagcaaagt ggatcgtgtc actatgttca ttggcgtcat
     8821 tgtaagttaa ttcgacactt tccacttctt tcaactggac tgtaaattca ggatggcgtt
     8881 tcaaaaactc ggtgattttg gaaaaacctt tataattgat cattgtcggt atcgttaaga
     8941 tatttaatga aagattgttg tctttcgtat aggtttcaag cacttcttgc atagcatggt
     9001 attgttctac gacatttttt acatatggga gcaataagct tccagcttcg gtcaattcga
     9061 tttttctatg ggcgcgttcg aataatttga cgtctaattc tgtttctaac gaaagaatct
     9121 gtttagaaat atttccttgg gtggtgaatt gtcgatcagc agttttggtg aaattcattg
     9181 tttcggctaa atcaagaaaa acttcgaatt tatggatatc catcatattc ctcctactta
     9241 ttccaataaa gaatacttta acacaaaaaa tgaattggtg taatcttctc tgtctgttac
     9301 aatctttctt gcagcgaaag caataattac agaaattttt ctgatttttt tcgcttcgta
     9361 gaaaaagaga ggagtttttt gatgccaact ggagttttga tcaatgtagg ttccgttttg
     9421 tttggcggac taattggcgg attagtagga aataaattaa gtgaacattt taaagcacag
     9481 ctgacgatgg tttttggtgt gtgttcgatg gggatgggaa tctattccat tgcgccgatg
     9541 aaaaatatgc cggctgttat ttttgcttta gtgataggaa cagcgattgg tttgattgtt
     9601 catttaggta atgggataaa taagggggct gcgctgatgc aagtacctat ttctaaaatc
     9661 ttccctagtg aaaagcttgg tatgacccac gatgaattta tcagcacatt agtgacggtc
     9721 attgttctat tttgtgcaag tggtacgggg atctatggaa gtttagattc tgggatgaca
     9781 ggagacagta cgattttgat ttctaaatct gtccttgatt ttttcacagc agctattttt
     9841 gcgtgtaatt tagggtatgt cgtttctgtg atagcaattc cccaattcat tattttttat
     9901 attttgttct tattggcaaa atttatttat ccattgacta caccagacat gatcttagat
     9961 tttaaagctt gcggcggatt cttgatggtc gctaccggtt ttagaatgat caacgtgaaa
    10021 atgttcccag tagctgacat gatacctgca atgatcgtta tcatgccact gagctggatg
    10081 tggacaaatt ggattatgcc actgttataa taaaaatcaa agagaagcca ttttttgctg
    10141 actaaaaagg atcaaatctt ttagtcagtt tttttgtatt tgacaaactg attttgaaac
    10201 tatattatat gttgtataat atagattgaa ataaaatatt acacttgaga atagaaggtg
    10261 acgtaatgca catccagatt ccaactgttc ttcttgatgg tactgtgcta gcagcattaa
    10321 gcaaggaaga tatgtatggg tatgccttaa caaaaatcgt acaagaaagt ctctctgtca
    10381 gtgaatctac actatatccg gttttgagaa gattgaaaaa agaaggatat cttgagacct
    10441 atgatgaacc ttttgaaggc agaaaccgtc gatattactc gttgacacca atgggaagtc
    10501 gtcatttaga agaaattatt gatgaatgga ttgtttttcg agattcttta gaccggttat
    10561 tggaggagag cacaaatgaa tgaatatttg aatgaagttg tggatcatct tcaagaaatt
    10621 cctgaagacg atcgttttga tttgatccag tattatgagg aatatttttt agattctgga
    10681 aaaacattag atcagatcat tgatgaatat ggtacaccaa agcagtttgc cttaaaatta
    10741 aaaatcagtt acttttcaga agaagaacaa gcagaaaacg agttagtgaa aaatccaaaa
    10801 agacaaattc gcctgatttg gttaatcatt atcggtttat ttgcttctcc tgtcctcatt
    10861 ccactagcac tggggtttat attggttatt gggggactat tggttgcttt actagcgctc
    10921 atttttagta tctatgtttt atgtatcagt atcttaggtg ttggcctatt catgatcatc
    10981 tcaggaatca gtgtgtttgg acaatcgatt ctgagtggat tattctttat cggtgtcggt
    11041 ctgttcgcca caggaagcgc agtatttatt tcgccactgt tgctgaaagg aacaaaaggg
    11101 cttttttatg tcatgatgaa ctttgttaaa tggatgggac gacgttttat tacaaaaaga
    11161 gatctgcatt cagcgggcgt ttgaaggagg acacataaat tgagattgaa acattttttg
    11221 attaccggta ttggtctgat gcttactggt ggcctcctag caggtgcaac ttatacatta
    11281 ggcgctcaaa agagtctgac ttgggataat ggtcctcggt tgatcgatat ggttactgag
    11341 acaaaaaaaa tcaaggatgc aaacaaaatc gtcatccatg cagaaaatca aacagtttcg
    11401 gtcatacgcg gattggattt tgagataaaa acgacttatg ataagacaga aaagccagaa
    11461 ataaaggaga aaaatggcgt gttgactgtg aatgcagtca aaaaatcaac aggtgcaatc
    11521 attagtttag aagacacaag tgcacaaatc actattgttg tccctgcaga tcatgaatta
    11581 tctgaattga caatcgaagg gaataatagt gcaattgata tcaacgaagt atcaatcaaa
    11641 aaagtgaata ctaagacaca tgatggctgg gtatcgttca atgaatttac aggaggaaca
    11701 ataactgcga aagatgaatt aggctcactt cgattagatg aggccaatgt ggataacttg
    11761 atcgtcacag gtgaaaacag caacttccat ctagaagaca gtttgatcag caaagaagga
    11821 aaagtcatcc taaacggtgg caatttatct ttgcatgaga tggaaaacag tggctatact
    11881 atccaaacgt ctggtgaatc ttatattgaa aaagactacc agccaataac aaatactttg
    11941 ttagaacaag gacagaaaaa gctaaagatc actgctaaag acacatcgat caatattacg
    12001 acggatcaag aggacaatga aacagattga tcctgtactc aactggatat tttaagaaaa
    12061 aacagcaaag atccttccaa tctttgctgt ttttcatcgc ttaacgcaca taactatagt
    12121 aaaataagaa agaaaatatc taaaaagaaa acagggtgaa agagtatatg aaggtagaag
    12181 aaggtcaaag agaaaagtta aaaacagaaa tggaaagatt acatacttat atcactcaac
    12241 taagccaaac cttctatgat ccagacaaag aaaaagtgat ggttaattac ccgaacaatt
    12301 cagaaggtcg ccaattagag caagtgtacc atgaagtttt caaacattta ttgacggtga
    12361 agaaagagct ggattattat tctttaccga ttatcgatac aggaatattg aagtatgatg
    12421 ggaagaagga acgatttatc tttaaatctg ttcgggaaga tttgcctctt tcagcaggga
    12481 tggatctaga ggtcttggta gaagactatt ttacagaaga aaagcactgg gttcgaacaa
    12541 aattggatta tttaccgcag gcagccggag ggacacaggc aagcggttgg tacatcactg
    12601 aagacaaaga gttagaacta gaaggtgtaa tggcgcgtat tcgaaaaaaa aattaaaaaa
    12661 gtaacattag atatttattg aaggataaag gagcgcagga agtagcatga gagaaaaaag
    12721 agcattttat atcaatggtt atgcagggat tgtcggattg gtcgtattag ctttagttgg
    12781 attgtttttc ttctatttag gtatgtggca agcgaaagtt tgggcacttt ttttaagtat
    12841 ttttctttgg ttgatcacgt tattgttgtt aagttcggca actgtagtca gtcctaatca
    12901 agcaaaagta attttgtttt ttggacaata tttaggtacg attcgagaaa atggcttttt
    12961 ccttacgata cctttggcac aaaaaatgac tgtttcgttg aaagttcgga atttcaatag
    13021 ttctgtttta aaagtcaatg atttggatgg aaaccccatc gaaatctcag cagtagtagt
    13081 ctttaaagtc gttgatacag caaaggcatt attcgatgta gcgtattatc aagattttgt
    13141 agaaatccaa agtgagacag ccattcggca tatcgctagc caatacccgt atgatacatt
    13201 caatgagaat gatttgacgc ttcgaggaaa cacgacggca gtgtctgacg agttgcaaaa
    13261 agagctgcaa gagcgtttag cagtagcagg tgtcgaagta atcgaaacac gcttgaacca
    13321 tttagcctat gcaacagaaa tcgctagtgc catgctccaa cgtcaacaag caaaagccat
    13381 tttgtctgct cgacaaacca ttgttgaagg agctgtcaca atcactcaaa tggccttaga
    13441 gcaaatcgaa gatggacaag aaatcaattt caccgatgat cggaaagtcc aattgatcaa
    13501 taatttactt gtctcaatca ttacagacaa aggaacgcaa cctgttatca atacaggcga
    13561 catgaaagac cgtaagtgac atactttttg taagatagga agaaggagtt taggacggat
    13621 tattgtcttg aactcttttt ttagttgttt taaagaaaat gacaaaattg aaagaagaat
    13681 gatagttgat tcgaaggcag aattgttcgc ttttttctat aatcagtata taaagtaaga
    13741 gagaagggga aaaagtgaaa aaacttctga tcggtctact tgttttcatc gttgtggtac
    13801 ttgcaggggt gaaaatcgca gattacgtag ttatgggtgg agaagaatac tatgtacaaa
    13861 ttacgacaga tggtacaaaa acagtttcaa aatcagactc tggacaagaa acagtcaact
    13921 atagatatat cctgcctggc tatacgaaag agggtaagga aaaagaactg gacttcaagg
    13981 gacaattgac tcgaccgctt cgtaaaggag catatttaaa aatcacctgg aacaaaaata
    14041 aaggtgtcac ttcgtatgaa gaagtcgaaa aaaaagacgt gccgaaaaca gcactggaaa
    14101 aactaattaa agggtaggga aagaaatgaa caaaatagtc gaagtacaga atgtaacaaa
    14161 agtatacgga aaaaacaatg agaaaaagac gcaagcatta agcggcatca gcttcgatgt
    14221 agaaaaaggc gaatttattg ggatcatggg cgcttctggt tctggtaagt cgacactttt
    14281 gaatatttta tccacattag ataaaccaac agatgggcat atccgaatca atcaaaaaga
    14341 tgtgaccaca ttaaaaggaa atcaactagc agattttcgt gcgaatgaga ttggctttat
    14401 tttccaagat ttcaatttat tagaaagctt gacagcacaa gaaaatatcg ctgttcctct
    14461 ttctttacaa ggcgtcagac caaaagaaat caagcaacgt gtccaaaaaa ttgcagaacg
    14521 tttatccatc tctcatattt tggaaagcta tccttctgag atttccggtg gacaaaaaca
    14581 acgggtcgcc gccgcacgtg cattgatcac acaaccaacg attctattag gggatgagcc
    14641 tacaggtgcg ctggattcca aaagtgcccg ggatctttta gatacgatgg acgaattgaa
    14701 tacaaaagat catgtatcga ttcttcttgt gacgcatgat ccgttttctg caagttattg
    14761 ccaacgtatt ttattcatca aggatggcgg tatccatcaa gaagtgaaac gtggagatca
    14821 aagccgtgat tccttctatc gagaaatact atctattctt ggaaacttgg aacagtaggg
    14881 ggagattgac tatgctttgg aaattatcac ttactgggat caagagccgt ttaagagact
    14941 acttggtgtt attctcagga ttagttatgg catctgctat tttttacatg tttgaatcaa
    15001 tggcaagcaa cgaagctttt ttgaaaagta atacgatcgt tgcggctaca gttatcattt
    15061 tccgattcgg ttctgtttta ctagggatca tcacattcgt ttatatttta tatgcaaatt
    15121 cctttttgat gacgatgcgg caaaaagact atgcaatgtt tatgatgctg ggagcaaaag
    15181 gcagaaaaat cgcacagatg attttttctg aaacattctt agtagggatc gcagctactt
    15241 tagtgggttc agctgtcggt gtaggattga caagtatcgt ccaccgatta ttagtggatc
    15301 agttagatct caatatttcg cattttacac cttttagtat tcacgggttg ttgatcactg
    15361 ttttgttttt tgctgttttg tttttattag ctgctgttgt caatgctttt tcgattgtca
    15421 aaaaaccgat tttagcattg attcgtgcgg atcagacacc aacaagaatc aagcagcata
    15481 aattcttgtt tttccttgaa gtagtactgg gaattgcttt tctaggctgc ggttactata
    15541 tgatgaaaca cgtggggacc tatcaagtct ttggtatagg ggtagcctta gttacgatcg
    15601 tattaggaac ttatttcatt ttccattctg tgatcatttt ctttttatca ttactaaaga
    15661 aatcagaaca gatttcattg aaaaaattga acaatttcac attgtcacag ttaaactttc
    15721 gtattcgtga atatacacaa atgctctcaa tggtcgctat gctgtttgcc ttggcactgg
    15781 gcgctttgac ggtaggatta ggttttaaaa atgaaattgc agacttgaca gaaaacaatt
    15841 ctggatatga tctagtgatg aataatcctc gggcagaaga tcagcaaaaa atcaaagaac
    15901 tttcaccaac actttctgct tcttatactc aaaaagaaga tgcgggaacg atttactata
    15961 atgcaagtga atttgcggct caaccattga tcatgataaa acatgaagat ggaacaccgc
    16021 cgaagatcac gaaagaaaaa gttcctgcta aaaaaatgaa tgaactgact gtacaagagg
    16081 aacttcgaag ctatgaattg ccggaacagc aagaaaaaga aatcaaggta gtcagtcaat
    16141 cagaatttga tcaactgaac ttgccacaat ccactttaca gctagttcag gtaaaagact
    16201 tccaagcaaa cctgaaacca atcgaaacac ttgttgctca aaataaaatg aacaatccat
    16261 ctttgcaaaa agtagagtac agcaaccaga aatatgaaat gtatgaagtg tataatggtc
    16321 tattctctgg ttttgaattt atgggattct tcctaggttt agctttcttg accatgcttg
    16381 ctagctgttt gatgttcaag atcttgtcag gaagcaagag tgacattgct cgttatgaaa
    16441 tgcttgaaaa gataggaaca cgacgcgggt tgcttaaaca atcgatccgt agagaagtag
    16501 gcgtgttatt cttagcacca ggaatcttag gtgtagtcca tgtcttattc ggattacaaa
    16561 tgtttggtct attcatgcaa aatccataca aagatatttg ggttccgttt acgatattct
    16621 tcgtattgta tttcatctac tatgtactga cgacatggtt atatactgga atcgtattga
    16681 aaaagagatt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa gcaaaaatag
    16741 ctcctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
    16801 ttgttcggaa aaagaggttg tgacaatatt tttgtcacaa cctttttttc acggtctttt
    16861 attagaatat agtaaatttt ttctcgttac tcacggaata agctgttaag aaaacggttt
    16921 ccatgtatgc tggaatcagt gcaaatgaaa gaaaagaggg tgcaaggaac atggaaatca
    16981 atgaacaaaa ttttggcgaa aactatcact tgattacgat cacgaatcaa aatgggacat
    17041 ctttgtctgt cagtgatctt ggggcacggg ttgttcattt tcaaacaata atcgataatg
    17101 aaaaacgaga gttgatctta ggttttgatg aagcaaaaga atatctagaa aaagaccctt
    17161 atatcggtgc ttcaatcgga cgtatggctg gacggattac gaatggcaca ttcaaattag
    17221 atgatgaaac gttcgaagta aaaacagacc cggacactgg tcattgttta catggtggtg
    17281 cacctagctt tgaagccaag cagtggcatt atacagtcat caatggtgaa aacgaagcat
    17341 cggtcatttt ctatcagact agcccagata tggaaaacgg atttcctgga actttagatg
    17401 tagaagtacg gtatacgctg acgaacgatg atacatggcg agtgacggtc caagggatca
    17461 gtgaccgcaa aacagtattc aatcctacaa atcatgtcta tttcaattta accggtgatc
    17521 cagcacagtc aatcgacgaa catatacttt ggttgaatag ccgattattt gctacgctca
    17581 ataaagatac aacgcctaca ggagaagtag tttcagtaag tggcacagca tttgacttcc
    17641 aacgtcctaa aaaattaggt gaggtctttg ccagtgattt tcgccaatca atgatagtgg
    17701 gcggcatcga tcatccattt ttgttgaaaa aacaatctgg tttactaaat gctgcggaat
    17761 tgacaagccc agatgaaaaa gtatcgatcc aagtaaaaac agatgcttca ggaatcgtga
    17821 tttttacagc aaattttgga gaagatggac cggaaatgcg cgggaataaa ttagtaaatc
    17881 atggcggtat cacatttgag actcaagaac tgccaggagc ggagcgactt gaaagttttg
    17941 gagatgtcac tctggaacct aaccaagtac gcgagtttgt aacggaatac aaaataaata
    18001 taaagaaata gaataaatta aaaagggagt gtgacagtgt cacatcccct tttta
//
LOCUS       DAHHZJ010000041        17732 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.042, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000041 DAHHZJ010000000
VERSION     DAHHZJ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.042"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            591..1280
                     /locus_tag="NBL02_001165"
     CDS             591..1280
                     /locus_tag="NBL02_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD2571865.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="NBL02_001166"
     CDS             1267..2475
                     /locus_tag="NBL02_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCD2571866.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="NBL02_001167"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="NBL02_001167"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCD2571867.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="NBL02_001168"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="NBL02_001168"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCD2571868.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="NBL02_001169"
     CDS             complement(6578..7273)
                     /locus_tag="NBL02_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HCD2571869.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="NBL02_001170"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="NBL02_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCD2571870.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="NBL02_001171"
     CDS             complement(8472..8663)
                     /locus_tag="NBL02_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HCD2571871.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="NBL02_001172"
     CDS             complement(8684..9574)
                     /locus_tag="NBL02_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCD2571872.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="NBL02_001173"
     CDS             complement(9561..10328)
                     /locus_tag="NBL02_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCD2571873.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="NBL02_001174"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="NBL02_001174"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCD2571874.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="NBL02_001175"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="NBL02_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCD2571875.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="NBL02_001176"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="NBL02_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCD2571876.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="NBL02_001177"
     CDS             14899..15282
                     /locus_tag="NBL02_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571877.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="NBL02_001178"
     CDS             complement(15450..16208)
                     /locus_tag="NBL02_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HCD2571878.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="NBL02_001179"
     CDS             complement(16226..17044)
                     /locus_tag="NBL02_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCD2571879.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="NBL02_001180"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="NBL02_001180"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCD2571880.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="NBL02_001181"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="NBL02_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCD2571881.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
BASE COUNT         4928 a         3686 c         3237 g         5881 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tgtattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tc
//
LOCUS       DAHHZJ010000042        17402 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.043, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000042 DAHHZJ010000000
VERSION     DAHHZJ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.043"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            19..456
                     /locus_tag="NBL02_001182"
     CDS             19..456
                     /locus_tag="NBL02_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571882.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            551..1435
                     /locus_tag="NBL02_001183"
     CDS             551..1435
                     /locus_tag="NBL02_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HCD2571883.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1659..4826
                     /locus_tag="NBL02_001184"
     CDS             1659..4826
                     /locus_tag="NBL02_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571884.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            4846..5391
                     /locus_tag="NBL02_001185"
     CDS             4846..5391
                     /locus_tag="NBL02_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HCD2571885.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            5419..5874
                     /locus_tag="NBL02_001186"
     CDS             5419..5874
                     /locus_tag="NBL02_001186"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HCD2571886.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            5871..6629
                     /locus_tag="NBL02_001187"
     CDS             5871..6629
                     /locus_tag="NBL02_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HCD2571887.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(6715..7455)
                     /locus_tag="NBL02_001188"
     CDS             complement(6715..7455)
                     /locus_tag="NBL02_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD2571888.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            7516..8769
                     /locus_tag="NBL02_001189"
     CDS             7516..8769
                     /locus_tag="NBL02_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCD2571889.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLIGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            9091..9318
                     /locus_tag="NBL02_001190"
     CDS             9091..9318
                     /locus_tag="NBL02_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCD2571890.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            9441..9767
                     /locus_tag="NBL02_001191"
     CDS             9441..9767
                     /locus_tag="NBL02_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCD2571891.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            9835..10548
                     /locus_tag="NBL02_001192"
     CDS             9835..10548
                     /locus_tag="NBL02_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HCD2571892.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            10858..11709
                     /locus_tag="NBL02_001193"
     CDS             10858..11709
                     /locus_tag="NBL02_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCD2571893.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            11830..13695
                     /locus_tag="NBL02_001194"
     CDS             11830..13695
                     /locus_tag="NBL02_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCD2571894.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            13709..15151
                     /locus_tag="NBL02_001195"
     CDS             13709..15151
                     /locus_tag="NBL02_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCD2571895.1"
                     /translation="MQSRFSEKFLWGGATAANQLEGAYDRDGKGLSVADAMPGGKQRF
                     AIIGSEEFDWTIDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPN
                     GDEGTPNEAGLEFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYE
                     RFAQTVLERYSSKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVA
                     SSKAVKIGHELRSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGE
                     YPAYTARTHKKYGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSE
                     GNLLGGVKNPFLEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDG
                     TIIDDYRIDYLRRHIEAMANAVADGVDLMGYTPWGCMDLVSASTGEMSKRYGFIYVDL
                     DDEGNGTLNRSKKKSFDWYKEVIASNGVNL"
     gene            15259..15960
                     /locus_tag="NBL02_001196"
     CDS             15259..15960
                     /locus_tag="NBL02_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCD2571896.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQEILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            16132..16611
                     /locus_tag="NBL02_001197"
                     /pseudo
     CDS             16132..16611
                     /locus_tag="NBL02_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            16649..17128
                     /locus_tag="NBL02_001198"
     CDS             16649..17128
                     /locus_tag="NBL02_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HCD2571897.1"
                     /translation="MFLDSGVVLKETELERIEYTDFGLGAIETEGLNLFVYENNERYC
                     GKELVLLPYQTCPEHRHPPRDENEGKKETFRCRKGLVYLYVEGKETKKLSCIIPKGQE
                     RYYTAKKEIILHAGDQYTIEQNIKHWFQAGATGAVITEFSSSSDDPSDSFTNPAIYR"
BASE COUNT         5792 a         2921 c         3755 g         4934 t
ORIGIN      
        1 gcacgtagaa gtagataaat ggagtggttt tgcaaatttc ttttatttct agaagttatt
       61 cacgtatacg gatttgtttt tctcacaaat aaaaaggcag gaagtacaat ggatcatttg
      121 tatataaaat atattcatca attttccgtt ttggtgttag gcacactaat tatagcgggg
      181 attgtcgcat ttattattca aggccgtgac atattaacag tacatcaaga gtttttcagt
      241 tatgcatttg gtattcctct gatcgctata aggtctgata acaaaaagtg gaagaaacgc
      301 tattacattc tcttgctatt tttgtgtttt tatctgccac ctaagatttt tactttatta
      361 ggtatacaat gcctatcatt ttcttttatt tcctatatca ttgttttaat tttcctcagt
      421 ttcatagtat ggatgtttta tcttgtcaaa aaataaatga atgtttcatg aacagtactt
      481 catcacagcc taattttttt ctttatgcta tcgttatatt aaagaaatca atcagaaggg
      541 aaagaaagtg atgaaaaaag ttttattagt ggtaaacccc agttcaggcg gtgaacaagc
      601 aaaggaattt gagcaattag cgatagcaaa attggaatcg gtgtttgatg aagtggtcgt
      661 cttgcataca aaaaaagcag gggatgcaaa aaactttact cgcgaagcgg ctacggaagg
      721 gtatcatagt gtgtttgtca tgggcggaga tggaacagtc aacgaaggaa tcagtggaat
      781 cgctgaacaa gaacatcggc caaatttcgg atttttccca ttgggtacgg tgaatgactt
      841 ggctcgagca ttagggatac cgctagaacc tgaggaagcg atcaatcatt tttcgattga
      901 atcagtcaag ccattggata ttggaaaaat aaatgacgac tattttatga atgtcgtcgc
      961 aattggctct atccctgaat caatcaatga tgtagatgcg gagaagaaga cgaaatttgg
     1021 aaaactagct tatttcatgt caggaatcaa acagttagct tccacacaat cctactcgtt
     1081 tcacgtagaa gttgatggaa agaaggaaga aattgagagc agtaccttgc tgataggact
     1141 aactaactca gtcggcggtt ttgaaacatt acttccaaat gcaaaagtgg atgatggtaa
     1201 gctccatttg gtttatttaa aagattcttc tttgatcgat acgcttaaaa cattgccaga
     1261 cctattaaaa ggagtagatg aatctacgaa aaatctagcc taccttactt gtgagcaatt
     1321 agttgtgtca ttagctgatg aggaagaatt gacgacgaat gttgatggag atgaagggga
     1381 taaattacct gtgacaatca aagtattgcc ctcacattta caggtttatt gttaaaatgg
     1441 aggttgtgac cgaggagttg gcgggagaac accgcttgtt cgggataagg gactgtgaca
     1501 agacttttgt cacagccttt tttttgtaca aaaaagaaat ttacacctaa tagaaaaatt
     1561 tttccataaa aatacatatt ttaaatatga aagtacaatt ggaaatagat taaaacgata
     1621 aaataaaatt gtcaaaacgt cagagaggat ggtggagaat gaatcaatgt gtaaaaaagc
     1681 tgattgcgtc gattttgttt gtcttccctc tcttgatggt gacatccgtt tttgccgatg
     1741 tagagaaaac aaacgaatca acgcaatcag agaatcaaag ttttgacaag actacttttt
     1801 cagaagaatc gattacttct gaaactacag aatctcaaag tgaggcttct actaataaaa
     1861 ggcaagaaga aaataagaca agtgagacct cagaagtagc tgaacaaacg tctgatagca
     1921 gtgaagcggc actggcaaga gctgtttcag cggatgggta cagcgaagct gccacaacag
     1981 aagaactggc gcagcttctc gcagacgaat cagtaactaa gatccgactg attcagcctt
     2041 tgactttaga cagggaatta gagatcaaac gagatatcgt tattgatttt ggtggattcg
     2101 ctcacaattt cggcacacac catatttata tcaatgagat acctcagtcg atcgagttcc
     2161 agaattttaa aggaacagct gcacatccag gtggattgac accaaatatc gatggaaatg
     2221 ccattatcat tgcttatatg accgattttt ggggaagcag atatcatttt acgggagaaa
     2281 taaaatttac cgggacactt gatttgtatg atgggtccaa attaggactg atttacgctc
     2341 ctcgtgcaac tgtcacatta gatggtgttt ccggtgtgtt ggatgtccaa ccagtgaagg
     2401 aaggactgaa tgccgctccg ggtaaagcct atttttgtag aagctatcaa ttgaatgtga
     2461 tcaatggtag tcaactttat ggaccgtatc tagggaaatt ctacggtttt ctagattcag
     2521 gagatgagac tggtagtgga aaaacagcgc caggaatcca tatcttaagc ggttcaaagg
     2581 tctctcttga ttatgatcgg gcagatggcg ttagcgatga aggagaggca gtcgatacgc
     2641 tacctggtaa tgtcatgttt gaagtcagtg gttcgggaag cgaattttcg gtaaatacaa
     2701 agatcaatat taccaatgac aacaatcgcg gaatcatcca aatgagaggt tcaggatcga
     2761 aagtcactgt atcgaatggt gggaaaatca ccatcaatac agcaactacg ggcggatttc
     2821 gcttgcaagg agattctagt caaatccatg tatcaagtgg tggacaaatc tcggtcaaaa
     2881 tggctggaga tgacgatcaa cccgggaaca atgggatgcg ttttgtagga aaaggccttt
     2941 ctttgatcgt agaaggagcc ggatcagtta tcgacatcga taagcagagt gggcgttcca
     3001 gcgcagttcg ttttgagaat ggtacgcaaa agctgacagt aaccaatgga ggctcattga
     3061 gggtaagaaa tacaggtgat ggcaaatctt acgtgaacag actcaatcaa ggaaatcaag
     3121 cgatccaatt ttatgacgct tctggttttc tagaaagtcc aggttcagct gattttacag
     3181 tcactggaga aaaatcaaat atcgatatac gcgcagattc cggagcaact gtagatacaa
     3241 ctggagatat tgatttaaat tttacggcag gtgaaaaaac ttatttagtc atggtcggga
     3301 aaacagggga agataagacc ggcatcatca gtggggatgt gttgaatgtg gacttagaat
     3361 ctcctaccta ttttgatttc caaaacaaac ggattggaag cgagaccaat ggcataggcg
     3421 gctgggtatt ccaaggaaat ggagattctt ctttaaagat cgcccattca gaaatcgctt
     3481 tatggcgaaa aacaggcgca acattcgata actttgaaaa agacccggat tatttttctg
     3541 atatgacaga cttatcgatc aatgggaccg acttaggcaa tttcgtatca agccctagtg
     3601 caggtttaac aaatgaattt gctcagacag gtgccggaat gaaaaattat aatcgaatca
     3661 gtgccaataa tcagctcccg atgattgaat ccttaagagt gccaacgaat gcagataaaa
     3721 aaatctatgg gcatgtcgtt gttcccgaag gtgtcgaggc aaagccaaga gatgcttgga
     3781 caggcgaagt agaagtgact ttacgtatta cttacgcaga tacgacgaaa tcatctgtag
     3841 aaattactgc tctcacgcaa ggacaaacag atgaaaatga aggggcagga tataacccat
     3901 ggggagaagg cgaacaaggc ggcttgttcg aagttcaagt acccaatgat gagagattaa
     3961 aaacggagga tcaagtgcaa gttgttgctg ctaaaaaagt aagaggtggc caagaagtga
     4021 cagaactgga ctctgccaaa acgacagtcg atgtgattcc tcctgaacct gctaatttaa
     4081 gtaccacatt gatcaatgca gcttctcgca cgatcacagg tacaggtgaa gcaggagcct
     4141 tggtttcatt gaaaaaaaat caagctgttt tgtcagaaac aactattgat gatacagggg
     4201 aatttgttct ttctatccca gaaaatcaac tttttgcagg agatgtatta gaaattgtat
     4261 taaacgacca agcaggagaa gctactgcta agggtgtgca gaacaagcca agtaccaacg
     4321 atgaaattgg gaatcacaat ccaagtgata cgccagttgt ttatcatgat gccccgcctt
     4381 ttgcacctgc tacgaagatc gtagtagaag gtggtctctc atttcttgcc ccttccaaac
     4441 tcgattttgg tcagatcaaa gcaactggtt tgacacaaga agcatttggc caaatggata
     4501 tacaggaaaa attatggatt tatgatcagc gagaaacgaa aaatccgtgg atcttaacga
     4561 taaaattatc tcagcctttc acaaatgatg caggattttc tatgcaggat gtcctatatt
     4621 tcaagaaagg agaggagtat caattagtga atgaagaaga actggaaatt gtcagaggac
     4681 aaaattcaac gaatgtgcaa gcagattatt cagaatattt aaataacggt cgggcgttta
     4741 aacttgtctt aaccaaagaa aatcagatag ctggtaatta ctcagcagag ataacatgga
     4801 cattagcaga tgcgccgaca gaataaagat caaggaggat gattgatgaa tcgaatatgg
     4861 aataaaagtc atcgaaacaa ctgctcttta cttatgatcc ttctccttgg attcatcctt
     4921 tggcagacac ctgtccatgc ctccgaattc aattttgcgg tagattttga attaccggct
     4981 aatcagattg ataagaacaa aacatatata aatgtaagga tggagcctgg gaaaacacag
     5041 gaaatcccct acacggtctt taaccgaaca gataaatcag taacagttga gacattgatc
     5101 cactccgcta cgacgaattc aaacggtgtg atcgaatatg gagaatcaaa aagtaaattt
     5161 gatgaaacat taagttatga tatgaaaaac ttagcaaaaa cagtgggaga aataactgtt
     5221 cctcctcaaa gtgcggtcac tgagacactt tcactgaaag cgccagaaaa agagctggct
     5281 ggtgtggtcg caggagggat cacgtttcaa gaaaaaaaag agaacgtttc taacaaaagt
     5341 acagttggtg tcgtcaatca gttttctcaa gtgaaagcaa tcttgatttg aaatagcgaa
     5401 aaagagtctt ccccagattt gaaattaaaa aaagcttatg cggatcaaag cgatggaaga
     5461 aatgtgatta aggcgaatct tcaaaatcca aatgcttctt atatgttcgg cttgactaca
     5521 tcggttgaaa tctacaaaaa agaaacaaaa gagccttacc tttcaataaa aaaagaaaaa
     5581 atagatgtag cacccaatac aaattttgat ttacttgtcc cattagaagg aaaaaaatta
     5641 gctccgggac attatcaagc ggttgtgaag ggtgcttgga aagaaaaacg ctggaatctc
     5701 cataccgatt ttgaaataac tgatgatgca gcggaccaat tgaatgaaaa ggatattgat
     5761 ttaaaaaatc agccagaaac atttgactgg cgatggctga tacttgttgg agtcattctt
     5821 attggcttat ttgctggatt atttatttta aaagaaagaa ggaaaaagaa atgaaaaaaa
     5881 caattgcatt aagtaccgtt ctagctggaa gtctattatt atgtacacaa agtgtttttg
     5941 ctgagaataa gagctcagcc actacagaag gaacgatttc tgtgaccaaa ccagatgctg
     6001 caaatcctga agacgttgtt ccagttcctc ctgttgatcc aaatgatcct gatactcctt
     6061 ttacacctga cgaaccaaat cagggaacat ccggattact gacgattgac caagctccag
     6121 atcttgattt tggaacaatc aagttaggta cagcaaaaac agtatatgct gcattacaaa
     6181 gcggaacgga cagtactgga gcacaaacac aagtacagaa tttcgtacaa gtaacggata
     6241 aaagcggaaa ttatgccggt tggaaattat cagtcaaacg aacagaattt attggtacaa
     6301 catctgatac gaataaacta actggttcaa aggtgaaatt caaaaacgcc gtgattcgaa
     6361 atagcgcaac taatagcagt ggaatgccat ctaccgtttc tggaactggc gctgaaggaa
     6421 tcgaaatccc agcagatcag gcagtagatc tagtaactgc aggagcaaat gaagggattg
     6481 gcacatggat ttattcatta ggagagaatg tggaacaagg gagacaatca gtggaattat
     6541 ttgttcctgt atccaattat gctgtagaca actacgtttc aacattcaca tggtcgttaa
     6601 cagatgctcc tacagaagag gaagcgtaaa atcatatcaa cgacttcagg gagtggagcc
     6661 gattcaaggc ccactccctg aaatctgcta tgcttacgtc tcagctctta ttcatcaact
     6721 cgaagcagtt ttttggaagt catggaaacg ttcctttgtc ttttcaaaga tctttcgacc
     6781 aacgagtaag gagtcaccgt tgaaaaattc taacacataa tgagcagaat caattttctg
     6841 caattgcgat aagttgatac agtaagattt taaaagtaaa aagtaagcat acttttctaa
     6901 ctctgtcttc aattgtttta aagaagtatt cactaattta tcttctgtag ttgttttgac
     6961 cataacttgg tttttcaaac ctttgatact ttcaatatat aaaatttccc gaggatacag
     7021 gacgagactt ccaccttttt gatcaataaa gattttatcg acatttacga tctgctgttc
     7081 tttccaattg atctgttcta gcacttgacg aatttgttca gataaattcc tgtcttttct
     7141 gatatatcca atcggaagga ttttccgatt gatgatctct actgcaaaat tcgtataagc
     7201 ggtgaaaaaa acaatattac tatgtgcatt ttttttacgt atttgttcgg cgacatcaat
     7261 acctgtgagt gattgcttca agtcgatatc gataaagaaa acatcgtgat tatcaatagt
     7321 gcccaactgg atcaactgat tgatttgaga gatagaagtg agctgcacga tattctcgat
     7381 atgatgattg accaattctc tgatgatcca ttcgctgtga aactggttat cttctatgat
     7441 aaaatacttt ttcatatgca ggctcctatt aaaaaaatat gtgtacattt tgaaacaaaa
     7501 caatggagga ataagatggt cgcaacagtg acactttatt tgacctttac tttgatttta
     7561 gatttaggta tctatttatt cttttcgaaa caactgctag taaaaaacag ctttggttgt
     7621 atagctttga tggtggtgac acttttactt cctatcccta aagtgagctt tccttaccta
     7681 attggcttat tattgatgtt tcttttttat tttactacga aaaacctgtt tatcagctgt
     7741 ttgatcagta atcttttttt tgctatcact ggactcagct tttttctggt ttttgatctg
     7801 atggattatt taggaaagag acatcttgga acagcaagtt tttttgcact acttatcgca
     7861 tttattttct atctattagt aaaaagtata gacaaacaga cgagatttct caacttgctt
     7921 tttacttatc caactgaaca ctattttgga ggtatttgta ttttattaag ctggggcgtg
     7981 atttacttgg tattgggaag tgttcacttc cgctcatcta attatctgct tctatctctt
     8041 ttgtcgatag tcatcagttt tttttctttg tttttttcca ttatgttgat cagtttccaa
     8101 gtaaaagaaa gacaacatca ggaatcgctt caactgtatc aaataaatga agaatattac
     8161 catcatctag aggaattcaa acatgattat aaaagtctac tgttttcatt aaaaattact
     8221 cttcaggaaa ataaaaaaga aacgttggaa tttttagaaa tattagaaga tcattctgca
     8281 gctatttttg aaaaaccgca aatacaacag ctaacaaata ttgtatcccc agctgtcagg
     8341 ggtgtctttt taaaatttct agaaaaagca gaatcggaga aaatcccgat ccaagtagtt
     8401 attcctgtcg aagtgaaaac aatcccgatt gatctggttg tttttattcg ctgtttatct
     8461 atttttattt ctaacgcatt cgatcatcgt gtagccgatc agagtccgat tacgatcaat
     8521 ctgctggaag aaaaagaggg atcacgtttt agtatagcga acaaggcaaa tccaacgaat
     8581 ttgacgctat cggagatggt caaaagagga aaaacctcta aaaaaaatgg cgggttaggt
     8641 ctatacagtg cgaaaaaaat gttggatgtt tacgaaaatg cagaactaaa aatcgaattt
     8701 tcaaaacaga atcactattt ttttgtagaa atgtatctgg catctagaaa gagcaaatca
     8761 gcctactgac ttactgtcaa aggataagtt tgaggaaagt ctacacggag atagagacaa
     8821 caacacagca tattcaaaag aaacagaagc gtgatgaccg ttgtcaccct tctgtttctt
     8881 ttgctatgta gttaaacaaa gtagttgaca cttccgctac ttagtattag tacgaaagta
     8941 tacttcatga tactaagtag ataagaagtg aaaagcggaa cccacgatcg tcaaaatgct
     9001 aactgctttg cttacaccag ataaagggaa gagcaatatc gcttatttta ggcgtggaga
     9061 agcaagcaaa tccctacgat cacaaaaaaa atgctgaccc agcagttaag agagttagaa
     9121 caagatgaaa gtatccatcg aaaagcctat aaccaagtcc cgcctaaagt agagtactcg
     9181 ctcaccgaag aaggaaaatc attaagagag attctagtag cgttgtctgt ttgggaagaa
     9241 gaacgtatcg aaaaacaaaa agcctcaagg gaagaagaca cgctactgca tacacatggt
     9301 ggctacctaa attattgatt ttctatgaca tttattttaa ataaaagaaa acaggagttt
     9361 ttttaaaagc atcatctatt ttttatgtaa aaaaatggta tgattaatca agtaagaagt
     9421 gattggaaga aggcaagcaa atgaatagta tggaaaaagt caaccagttt agagatgaac
     9481 gtaattggcg acaatttcat aacgaaaaag atttggctat ctctatttct ttggaagcat
     9541 ctgaattgtt agaacttttt caatggaaaa cgccggaaga agtaaaagaa acacaattag
     9601 aacgaatcaa agaagaactg gcagatgtat tgatctattc atacatgatg gcagacaacc
     9661 ttcacctgga tctagatgag atcattgaag aaaaattgat caaaaataat ttgaaatatc
     9721 ctgtagaaaa aagcgcaggt gtacgaaaaa aatacacaga gttgtagaaa tgtgtcattc
     9781 ggctgttgga aagagcaaaa gaaaaacagt aaaatgagtc tggagggaga gaaaatgaaa
     9841 aaaaaaggaa aagcaaaaag atggctagtc aatatcctct tgttcctctt gttattagta
     9901 ggactagctt tgattttcaa tgaacagatc aaggattatt ttgtcagaga gacaggagat
     9961 aaatatgcta tagcgaatgt aacaaaagaa gatctgaaaa agaataacga taaagatgtc
    10021 agttttgact ttgatgcggt agaaccaatg acgacagaag gggtcatgag gtcgcaaatg
    10081 agaggaacag atctaccagt gattgctagt atcgcagtcc cttcagtttc aatcaattta
    10141 ccggttttca aaggcttgga taatacgtca ctactttacg gagcaggaac attatctcca
    10201 gatcaagaaa tgggaaaagg aaattatgcc ttagcaagcc atcgtgcaac gaaccctgaa
    10261 ttgctgttta caccgctaga aaatttagag atgggtgcca agatctattt gacagattta
    10321 gaaaatgtgt atacatacaa aacatttttt aaagaaaaag ttgctcctac agatacccaa
    10381 ttactgaatg aagtcgaggg aaaagaaatt gtcacactaa ttacttgtgg cgacatggac
    10441 gccgtcacgc gactagttgt acaaggagaa ctggaaagtg ttacatcgat aaaagatgcg
    10501 acagatgata tgagatctgc tttcaatcta gagacaaaaa cattttagga tactgggaca
    10561 taagtcgtta cagattaaaa acagccaaag gatatgaatc gataaggaaa aaccaatttg
    10621 ctgaaaaaaa gagaaacgca aagaggtgca tctctgattt ggttgttttc tagatagact
    10681 tgtgttccag tttttttatt ttttatatga aaaggcttgc ataaataaaa attcgatggt
    10741 atgatgattt tgaataagaa atggattgtt actgtacggc aggcaaaacc agaattcagc
    10801 taagaaacag taggcgaatt ctaggttttt ttatttttct tgagagggcg aagcaaaatg
    10861 aaaattgata aaattttgaa taacaatgtc gtcatttcga aaaatggatt cggggaagaa
    10921 gtcgtttgta tgggaaaagg actagctttt caaaaaaaga ttggggacga gatttcgcca
    10981 gaagcggtcc aaaaagaatt tgttttaaga gattcttttg ctaaaaacca attccagcag
    11041 ctgatggctg atattccggc agaggagatc gaatgggtca aacaagtagt cgaactggct
    11101 gaagataaac taaaagttga attttcgcct aatatttatc tgacattgac cgatcatctt
    11161 cattatgcga tcacacgagc aaaagaaaat atccaactgc ctaatccatt attatttgaa
    11221 acaaaaaaat tttatccgaa agaatatcaa gcagcgaaag aggctctttt gttcgtgaaa
    11281 caaaagacgg gtgtggaact ttctgaggat gaagccggtt ttattgcctt tcattttgtg
    11341 aatagccaac aaggaaacga agacatgcaa gtgacgatga ccgctaccac aatggtaaga
    11401 gatatactga gtatcatcag taaattcttc ggcatcgtgt tcgatgaaga atctttgaat
    11461 tatcagcgta tcattactca tctgcagttt tttgcccagc gttatctcaa aggggaagga
    11521 tctgacgagc aagatgaatt tctctacgca ttagttcaag gaaagtatcc aaaagcgttt
    11581 cattgtgtcg aacggatcaa tgagtatctt cgacaaacgc aaaaacagga aatggggatc
    11641 gcagagcaga tttatttaac gattcatatt caaagagtag taagtgagaa aaaaagcatc
    11701 caatcataag gattgttact gtatggcagg caaaacctgg attgaaaaaa taggttcacg
    11761 aatgtttgtg actgtttttt tcaatctggg tttttatttt ttaaataaaa aagaaagaag
    11821 gaaaaaacaa tggataatca agcagttggt cgtcgtgtat gggaagcggt aggaggacaa
    11881 aagaatgtca aaagtttagt acattgtgct acgcggttgc gattcagatt aaaagatgaa
    11941 tctttagcag atacgcaaaa gttaaaagaa gacccggacg tgatccaagt cgtacaaagt
    12001 ggcggtcagt atcaagtagt gattggcagt aatgtagcag atgtttatca atcgatcgtg
    12061 gatgaggaag gtcttgcagt ttcggaggat ggaaaagaag agtccaaaaa tattttgaat
    12121 cgtctgatcg atattatctc tagtatattt acaccttttc taggggcgat ggcagccgcc
    12181 ggggtattga aaggattctt atcattggca acggtgatgg gctggctgac agctgatagc
    12241 ggtacttatc aaatattgtt tgctgcagca gatggtgtct ttaccttctt acctgtcatg
    12301 cttgctttta cagcagctaa aaagttcaaa gccaaccagt ttttagcagt agcaattgct
    12361 atggcacttg tgtatccagc tatcacagca gttgctggtg cgggagaagc tatcagtttc
    12421 tttgggatcc cagtgattct ttcaccatct ggttatactt cttctgtcat accaatcatt
    12481 ctagccgtat gggttcaaag taaattagag ccattcgtaa aaaaagtgat tccgcaattt
    12541 ctgcaaatga ttttagttcc gcttgtcgtt ttagtagtga tggttccatt aacatttctt
    12601 gctttaggac caatcgggac ggttgccggt aatgctttag gcggtctctt caactcgatt
    12661 tatggattta gtccaatcgt agcaggtcta atcatgggaa gtttatggca agtattcgtg
    12721 atgttcggca tgcattgggg attcgtaccg atcatgtttt tgaatatcga acaatatggg
    12781 tttgatgtat tgatgccaat gcttcttcca gccatcttgg cacaaggtgg ggctgcttta
    12841 gctgtagcat tgcgaacaaa agatacgaaa cttcgagcat tagggatttc ttctacagtg
    12901 acttcgttat ttggaatcac tgaaccaact gtgtatggtg tgacattacc tttgaagaaa
    12961 ccattcattg ccgcttgtat ttctggaggt attggtggag ctattatcgg attttccgga
    13021 gtaaaagctt tctccagcag tttggtcagc ttattgacga ttccgacttt cattaatacg
    13081 gtggatggtg tagagtcaaa tgtgacagta gcagtaatag ctacaggaat tgcttttgtt
    13141 cttgcattcg taggtacatt gattttagga tttgacgaac aaacacaaga caatcaatta
    13201 gaaaacaaac atgcgaatgc aggagaaccg attacttcag cacggcatac cttaaaaagt
    13261 cctttaacgg gtaaagtact gccattgagt gaagtaccag accaagtttt ttcttctggc
    13321 gtgatgggaa aaggtatcgc tattgatcca gaagtaggag aactagttgc acctgcagac
    13381 ggagagatca cgactatttt tccaactgga catgctgtag ggatcacaac gactgacgga
    13441 gcggagattt tgattcatat cgggatggat acagtagaat taaatgggaa tggctttgag
    13501 atcttagtca aacaagggga tctcgtcaaa gcaggagatt tattgattcg ttttgacatc
    13561 gaagcaatta gagcagctgg ctatagcgta atcactccag ttgtcattac gaatactgat
    13621 gcatttgccg atatacttga attagaccaa aaagagatta ttgcaaacga agatgttctg
    13681 gcaattgtaa aataaggagg aaatcaggat gcaatcacgt ttttcagaaa aatttttatg
    13741 gggtggagca actgctgcca atcaattaga aggggcttat gatcgagacg gaaaagggct
    13801 atccgttgca gatgctatgc ctggaggtaa gcagcgcttt gcgattatcg gcagtgaaga
    13861 atttgactgg acgattgatc aagaaaaata tatttacccg aatcatcgag gaattgatca
    13921 ttacgatcgt ttcaaagaag atcttgcctt gtttgcggaa atgggattca aatgttatcg
    13981 tttttcgatt gcctggacac gaatttttcc aaatggagac gaaggtactc ctaacgaagc
    14041 gggattagaa ttttatgatc agctgattga tgagtgctta aaatatgata ttgaacctgt
    14101 gatcacgatt tctcattacg aaatgcctct tcatttggca aaagaatacg gtggctggaa
    14161 aaatcgaaaa ctgatcgact tctatgaacg atttgctcag acagtattag agagatacag
    14221 cagtaaagtc aaatattgga tgactttcaa tgaaatcaat tcggcctttc actttccagc
    14281 tttaagtcaa ggattagtaa aaagcaatgg agcaggagag taccaaaata ttttccaagc
    14341 atggcataat caattcgttg caagtagcaa agccgtaaaa atcggccacg aattgcgttc
    14401 ggatatccaa atcggctgca tgattatcta tgcaacaact tacagcatcg atgccaatcc
    14461 agtcaatcag gcagcaacaa tgatccaaaa ccaagaattc aattttttct gcacggatgt
    14521 ccaagtacgt ggagaatacc ctgcatacac agcgcgtacc cataaaaaat atggagtcga
    14581 tccggaaaaa ttagagcaaa cggaaaaaga cttccagttg ctaaaagaat atccagtgga
    14641 ctacatcgga tttagttatt atatgtctac ggcaatcaat gagactgatc cacaagcagc
    14701 aacttcagaa gggaacttgc tgggaggagt aaaaaatccg tttcttgagg caagtgaatg
    14761 gggctggcag atcgatccag aagggctgag aatcgcattg aatgaattat acaatcgtta
    14821 ccagaaacca ttattcattg ttgaaaacgg tcttggagcc attgatcata tggaagagga
    14881 tggcacgatt attgatgatt atcggatcga ttatttacgt cgtcatatcg aagcgatggc
    14941 taatgcagta gcagacggtg tagatttgat gggctatacg ccttggggat gtatggatct
    15001 tgtcagtgca tcaacaggag aaatgagtaa acgttacggc tttatctatg tggatctaga
    15061 cgatgaagga aatgggacac tgaatcgatc aaagaaaaaa tcttttgatt ggtataaaga
    15121 agtaattgcc tcaaatggcg taaacttgta attaaagcag ctgaatgaaa aagaacacac
    15181 ttctcacatg tgatttgtgt tcttttttta ttttttatgt gctattcttt tagtaatagt
    15241 aaagcaaagg gtgacgttat gaaaaggaca cgtgtactat acttggaagt cgcagataaa
    15301 attaaagaag atattttctc aggaaaatat ccagtcggtt cgatgttacc gacagaaagt
    15361 gaattagaag aactgtttca agtcagcaaa atcactgtcc gaaaggcaat cgaactgctg
    15421 gcatcagatg aatatgtcgc aaagaaaagt ggtcgtggga cgacggttct aagcaatcgt
    15481 ccttacaatc gattatccaa agcggcaagc tttacgcaaa tcttggagaa ctcccgatat
    15541 gaagtccgga aagaaacgct ggatttgcaa ttggtccata taaagaagga agatcctttg
    15601 ttttcctttt tcggtgagca agctgtttgt tttaagcgct tatattattt gaacaatcag
    15661 ccttatattt attttgttca ttatcttcca caagaaatga tgcaattcag cagagaagaa
    15721 tttggcaaag aatctttgta tcgtttgctg acgaaaaaag gatatactat tgactcattt
    15781 tctgatgatt tctcggcagt atttttaaca gaagaagaac aagaaattct gaaaacagac
    15841 gcacagatcg gcatcaaacg gatacggaaa tcgtctacgc aaattgggga aatgattgaa
    15901 tattcggagg cggtctacca tacggagctg catccttacc atattgatta tgaaacataa
    15961 ataaaaaaag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca gccaaacaaa
    16021 aatagcttct catattagct tctcatattt ttcgtttggt taggcttatt accgcagata
    16081 cccggctgtt tctggattac catttattct ggataagggg gtgtggcacg attttcagaa
    16141 ggaatctatg aaccaatcac tgtattgatt ttagcttcga tggtaacccc attcggcttg
    16201 acgatcgctt atttcttagg gaaaatcatt cgtaaaaata tattcaatcg tcaagaaatc
    16261 gatacattga agaccgcttt tcctatgggg atctgtcaga tcacagaggg atgtttccct
    16321 atcgtattga acgacttggc acgcaatgtc atagcaacgg gagtcggtgg ggcagtcggc
    16381 ggtggattga gcatgttttg gggcgcagat agccgtattc cggcatcggg aatgtttgca
    16441 gtcgctacga tgaccagacc atgggcattc attggtgcat tgttagctgg ttcttttgct
    16501 acgggaataa ctattttatt attgaaaaaa ccagttgacc caaatgcaga agtcatccaa
    16561 gaagaaaaag aggaagaaga gatatcttgg gatgacttga caatcagtta ggaggaacag
    16621 aatggattac aagaaaagag tacaacaaat gttcctagat tcaggagtag tattgaaaga
    16681 aacagaatta gaaagaatcg aatatacaga ttttggatta ggagcaatcg agactgaagg
    16741 attgaattta tttgtttatg aaaataatga gcgatactgt gggaaagaac tggtattatt
    16801 gccttatcaa acttgtccag agcatcggca cccacctcga gatgagaatg aaggaaaaaa
    16861 ggaaaccttt cgttgtcgaa aaggcttagt atatctttat gtcgaaggaa aagaaacaaa
    16921 aaaactttcc tgtattattc caaaggggca agaacgttac tatacggcaa aaaaagaaat
    16981 catacttcac gctggagatc agtacacgat cgaacaaaat attaaacact ggttccaggc
    17041 aggtgcaaca ggcgcggtta tcaccgaatt ttcttccagc agtgatgatc caagtgatag
    17101 ttttacgaat ccagctattt atcgttaata caaaggtttg ggaacaagct gatgaaccaa
    17161 aatgatcaaa tttcgattat ttggttcgtc ggctttttta tttagagaaa catcaggcgt
    17221 tggtgaaaca taacacgaga ataaaatgga tttttcatta ataggagaaa caagtgtgtg
    17281 actatttaaa taatgcgtgt tattgacatg caggacgaaa tcccaatttc ttctataagt
    17341 aagtcgtttc aattttatat ttactagtta tttatttttt tggttactgt aaaatagctg
    17401 aa
//
LOCUS       DAHHZJ010000043        17400 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.044, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000043 DAHHZJ010000000
VERSION     DAHHZJ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.044"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(307..1440)
                     /locus_tag="NBL02_001199"
     CDS             complement(307..1440)
                     /locus_tag="NBL02_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCD2571898.1"
                     /translation="MLLNFKMTLTMLAFSLLVTGVILVSFFFLRNNLEGISIASTLGE
                     DVYFMEEGKTVQAETQMEASKSSNNQFMTISMIDKAYQDELFKYLPAMVLVICSAVML
                     LTFILWLVLRKIYSNQILAITRDLTFIDQVPKNTVKDEALNAAFNKLKDKFDGHLEDY
                     RRLNSYLTHEQKNAIAILRTNLEIERQHADQGNLMLLDRMTDSIDDILTLSNNSEDLS
                     TEVVDVSLICAEVCDAYKMNYPNLLFAFTEDQPTLIHGRDRWIYRAVSNLVDNAIKYG
                     EGKLVIVTISNQKGSVIIEVKDQGIGIPQEKQASIFSHQYRVHGLKQDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            complement(1431..2099)
                     /locus_tag="NBL02_001200"
     CDS             complement(1431..2099)
                     /locus_tag="NBL02_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD2571899.1"
                     /translation="MRVLVVEDNRELVLSVKKGLEQENISVDVAYDGESGEEKAFINS
                     YDGILLDLNLPKKDGLSVLQSLRAEEVESPVIIITARDEIEERAKGLDFGADDYLVKP
                     FELLELKARIRAVIRRYHGRALPQISIGALKIDPLGRRVSYQKTEVVLKPKEFDILLC
                     IAQRHPAVVSSEEISEHVYDEDFDPFSSVLRVHLARLKKKLAEAAEKEVLKTIRAKGY
                     QLCY"
     gene            complement(2555..3541)
                     /gene="lacD"
                     /locus_tag="NBL02_001201"
     CDS             complement(2555..3541)
                     /gene="lacD"
                     /locus_tag="NBL02_001201"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HCD2571900.1"
                     /translation="MNEEKRQYIKQLCNEDGVIGALAIDQRGALKKMITKFKGSEAEE
                     SEIVEFKEIVSSQLTKYASSILLDPEYGLPAADVRAKNAGLLLAYEKTGYDASTPGRL
                     PDLLSIWSVKRLKEAGADACKFLLYYDVDEAEEINNQKHAFIERIGSECQAENLPFFL
                     ELVSYDANISDATSKEYAIKKPHKVNDMMKEFSDPRYGVDVLKVEVPVNMNYVEGYGT
                     DEVVYTKAQAANFFKEQAEATNLPFIFLSAGVKAELFQETLRFAKNAGSTFNGVLCGR
                     ATWADGVESYIVDGKEAAMEWMNTQGRKNIEELNAVLKETATPVEVLTSSLS"
     gene            complement(3554..4480)
                     /gene="lacC"
                     /locus_tag="NBL02_001202"
     CDS             complement(3554..4480)
                     /gene="lacC"
                     /locus_tag="NBL02_001202"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HCD2571901.1"
                     /translation="MILTITMNPSIDISYPLETFKLDTVNRVRNVRKTAGGKGLNVTR
                     ILKQLDTEVIASGLIGGFLGEEIKKDLTQNEIAHDFLPISGETRNCIAVLHDGKQTEI
                     LENGPVITEQEARAFLQHFEKLAVKAEIISFSGSLPEGLPADFYRQMLQVCQPKPVVL
                     DCSGTALREVLRGSVKPKLIKPNTEELAGLLGREISTELSDLKAALSDSMFDEIEWIV
                     VSMGGDGAFAKHHETFYCVTIPKIDVVNPVGSGDATVAGLTAAIQQGLSDEAVLKQGN
                     VLGMLNAQEATTGYVQLANYQKLYDQILVEKV"
     gene            complement(4493..5008)
                     /gene="lacB"
                     /locus_tag="NBL02_001203"
     CDS             complement(4493..5008)
                     /gene="lacB"
                     /locus_tag="NBL02_001203"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HCD2571902.1"
                     /translation="MKIAIGCDHIVTDTKMAVSDFLKSKGHEVLDMGTYDFTRTHYPI
                     YGKKVGEAVTSGEADLGVCLCGTGVGINNAVNKVPGIRSALVRDMTTAIYAKEQLNAN
                     VIGFGARITGEFLICDILEAFINADYQETEENKKLIAKIDHVEKASDKQSDPHFFDEF
                     IEKWDRGEYHD"
     gene            complement(5027..5455)
                     /gene="lacA"
                     /locus_tag="NBL02_001204"
     CDS             complement(5027..5455)
                     /gene="lacA"
                     /locus_tag="NBL02_001204"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HCD2571903.1"
                     /translation="MRVVIGSDSDGMELKELIKKKLIKEGITVVDKSETASEDFVEST
                     LAIANDLNENEGSLGIAVDGYGAGSFITATKVKGMVAAEVSDERSAYMTRKHNNSRMI
                     TLGSKIVGDELALNIVKEFLQANYDGGRHQVRVDMLNKMA"
     gene            complement(5475..6248)
                     /locus_tag="NBL02_001205"
     CDS             complement(5475..6248)
                     /locus_tag="NBL02_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCD2571904.1"
                     /translation="MLKTERQRSILAMCDRYGTITVKFIQEVLNVSDMTIRRDLEELA
                     QKKKLLRVHGGAQKIDYLEMSTSIEANELSHTEKKKLHTKEKEYIAKNAAAHINQEDD
                     TIFLGSGTTIELMTRYIKPSSLRIVTNSLPVFNLLSEIKRFELYLIGGLYREKTGAFV
                     GSIANEAVQKIGIKKAFVGVNGLKGSEVSNFSIEEGTLQKIVLDNAAEKCLVSDANKF
                     DHSDFYNFYDLRKIDKLFTDSTLSAELKSYYQQFTKIIQ"
     gene            6413..6892
                     /locus_tag="NBL02_001206"
     CDS             6413..6892
                     /locus_tag="NBL02_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCD2571905.1"
                     /translation="MNEFFFKDTVYVSDKQSQEEVFEEVYQDLLKKELVTEDFLENLL
                     ERERNYPTGLDLSPVSKILPDIAIPHTESEFVRTTRIIPIKVKNEIPFYNMISPSDQI
                     SASFLFMILNENGEAQAGMLANIMDFINSVDEEKLFSFFKLEDTEAIYHFLEEHFYI"
     gene            6949..7251
                     /locus_tag="NBL02_001207"
     CDS             6949..7251
                     /locus_tag="NBL02_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003063077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HCD2571906.1"
                     /translation="MIKILAACGAGVNSSHQIKSALETELKNRGYQVTCDAVMIKDIN
                     EEMLSHYDIFAQIANTDLGFDINIPVVDAGAILYRIPAMAEPVYQSMESAIQSLNK"
     gene            7273..8730
                     /locus_tag="NBL02_001208"
     CDS             7273..8730
                     /locus_tag="NBL02_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCD2571907.1"
                     /translation="MNTIIDIANKVFTPLINLGAAPLMTIVLTVIALFFKVKPSRALE
                     GGLKLGIALTGIGAIIGILTTAFSDAMTAFVERTGISLNITDVGWAPLATITWGSPYT
                     LYFLLIMVIVNIVMLAMKKTNTLDVDIFDIWHLAIVGLFAIFCGANLFIASALVIFIG
                     VLKIWNSDLMKPTFNDLLNAPDSNPMTTTHMNYMMNPIIMVFDKIFDKIFPWLDKYDF
                     DAAKLNSKIGFWGSKFAIGIYLGIFVGLLAGQTPTQIFSLAFTAAVCLELFSLIGAWF
                     IAAVEPLSQGITDFANKRLKGRTINIGLDWPFIAGRAEIWAAANVLAPIMLLEAIILP
                     GNKLLPLGGIIAMGVTPALLVVTRGKLIRMIVIGAIELPLFLWSGTLIAPFVTQTAKA
                     VGAFPAGLNGSAMISHTTMEGPIEKFLGYLVGNASQGQIEFVIYAALALGAYLLIFIW
                     YARQMKKRNAAYAAEKEENASPATAHNMTYAAEAK"
     gene            complement(9323..10075)
                     /locus_tag="NBL02_001209"
     CDS             complement(9323..10075)
                     /locus_tag="NBL02_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCD2571908.1"
                     /translation="MLKTDRFQAIMSLLEVNGTVRVSDIMEKFSVSDMTVRRDLDEME
                     KQGLLERVHGGAKLKDFYRHVELSHREKQIMLQEEKQRIVEKANEMINDGDTIFLGPG
                     TTIELMSKIIQGDSLRIVTNCLPVFEELNKRKGNRKIYLLGGEMRETTQCFVGEITNK
                     SLQDMNFHKAFFSCNALNEQKIMTSTFEEGQTQQIALNNSVERFLLIDHSKIGKRDFS
                     VYYNLNDVTVVITDDDEQKHYEKIEKEEKYVV"
     gene            complement(10290..10526)
                     /locus_tag="NBL02_001210"
                     /pseudo
     CDS             complement(10290..10526)
                     /locus_tag="NBL02_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
     gene            complement(10536..11500)
                     /locus_tag="NBL02_001211"
                     /pseudo
     CDS             complement(10536..11500)
                     /locus_tag="NBL02_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(11706..13388)
                     /locus_tag="NBL02_001212"
     CDS             complement(11706..13388)
                     /locus_tag="NBL02_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HCD2571909.1"
                     /translation="MNTIIKQIEKGKPFFEKISRNIYLGAIRDGFLTAMPAILFSSIF
                     IMIAAVPEVFNVTLPEAFSTWLWKVYGYSMGLVGLLVVATTARCLANSMNRQLSDSGK
                     QINEVSVMLASICGFVMMAADQIDGGIATDYLGTKGLLAAFVSAFVTVNVYKFCVKRD
                     LTIRMPKEVPGTITQMFRDIIPFSIAVFIAVLIDTGVRQFFGTSFAAGVIQLLQPLFS
                     AADGYVGIAIIWGAMAMFWFVGVHGPSIVEPAIAAIIYANVETNFELFKNGAQASKVL
                     TVGLGNFVGTMGGTGATLVVPFLFMLFAKSKQLKAVGKASFIPVSFAVNEPLLFATPI
                     ILNPYFLIPFLLAPIANVWIFKLFVDVLGMNSFISVLPWATPAPIGLVLGTGFSVLAI
                     VLAVVLIVVDAIIYLPFVKAYDSELVEQELKNIAAEEVKEELVEEIQEVGATAAVIVD
                     EAKASVGEGKRVLVLCAGAGTSAMLANALEEGAKETGVNLSAKAGAYGSHYDMMKNFD
                     LIVLAPQVNSYLDDLRKDADKLGIKVIATKGAEYINLTRDPKGAVDFVLKQF"
     gene            complement(13399..13722)
                     /locus_tag="NBL02_001213"
     CDS             complement(13399..13722)
                     /locus_tag="NBL02_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCD2571910.1"
                     /translation="MDRKELQLLGFEIVAYAGDARSSLLTVLKEVREGNFDNVERLLA
                     DADENLTLAHNSQTKCLAEEASGNEMEVGVIFIHGQDHLMTTILLRELINDFVELYKE
                     KFAKA"
     gene            complement(13745..15151)
                     /gene="lacG"
                     /locus_tag="NBL02_001214"
     CDS             complement(13745..15151)
                     /gene="lacG"
                     /locus_tag="NBL02_001214"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HCD2571911.1"
                     /translation="MNYKTLPEDFIMGGATAAYQAEGSTKEGGKGQVAWDTFLVEKGR
                     YTADPASDFYNQYPVDAELCKKFKIDSIRMSIAWSRIFPKGYGEVNQTGVEYYHKVFA
                     ELNKHGIIPFVTLHHFDTPKELFDNGDFLNRENVEHFVNYAQFCFDEFSEVHYWSTFN
                     EIYPVASNQYLIGSFPPEVQFDIEKTVTCLHNMMYAHAKTVNIFKDGGYKGEIGVVHS
                     LETKYPASDKEEDKHAAKLDDALSIKFLLDATYLGYYSDETMAHINEILAANGQSADI
                     DSNDFREMKKAANRNDFLGINNYGSHWVKEYKGESTLHYNTTGEKGSQVYCIKGIGER
                     IVREDLPKTDWDWIIYPKGLYDLLVRIQDEYPNYNKIYITENGLGYKDNFEDGIILDD
                     DRIDYLRQHFNAISDAIKMGVNVKGYFIWSLMDVFSWVNGYTKRYGLFYVDFDTQKRY
                     PKESAYWYKFVSETKTII"
     gene            15618..16625
                     /locus_tag="NBL02_001215"
     CDS             15618..16625
                     /locus_tag="NBL02_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCD2571912.1"
                     /translation="MVTIRDIAKKSGYSTATISRLFKGDESLSITSDTKNKIIVTAKS
                     LGYDLTKIKTTLYKIAILFWISEEQLKKDVYYQHVKDDLKNYSKKANLDLIFISKNDG
                     VKSIPDDITGFIAIGQLTKKELYYLNQKGYKGVIRGINPLPEHFDTVEPDFHEMARQA
                     INYFINEGFTKIGFIGGNFFNLETGKEEIDSREYAFRYHLEKSGLLNEKYIFSQGSFT
                     VDEGYQLTLKMKAELNEDMPEACFIPSDTIAAGVLQGCHELGINIPKDMAVISINDDD
                     IAKFVAPPLTTFKIDIEEIAKTCVDLLSDQLVYSRDISKKTLIGAKLTVRKSFIPKEE
                     V"
     gene            complement(17019..>17400)
                     /locus_tag="NBL02_001216"
     CDS             complement(17019..>17400)
                     /locus_tag="NBL02_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-dehydro-3-deoxyphosphogluconate aldolase"
                     /protein_id="HCD2571913.1"
                     /translation="NSLRYLEFREGAFDFQTSQQNLLTIPGAMSPTEIFKMFEMGADI
                     VKVFPANCLGPEYFNQVQAPLGSLPLMAVGGVDQTNAQNYLNNGASYVGIGSKFFEKS
                     AVHQLNYERLMELAESFIDSLRVE"
BASE COUNT         5218 a         3674 c         2860 g         5648 t
ORIGIN      
        1 acggttgtgt aaaaatatca ttttagagca tgaaaaaaag agtggatttc tccactccaa
       61 aaaagtctaa cttttggggg tcactacatt tttgatcctt tttatttgat taattatatc
      121 cagctggata ataattttgg gggcaacatt tctatcttac tattatcttc ctaaagtagt
      181 agactttctt attgggaaaa attacaacaa aaaggagtgg cttagttgac aactcctttt
      241 tgtttacaaa tcaattggat atctataaat tgtattttct aacgaatttt atagacatct
      301 gatttgtcaa attaagctag acaatatgtc tttatctatg cagctcaaaa atacttccaa
      361 tggtgttcga taatttaatg attttcttgg aatatggttt cttttcgagg caacggattg
      421 aataaaggtt tcatccgtgt ccctaaagtc cattgtttta ggtaagccat cctgcttgag
      481 cccatgcact cggtactgat gagaaaaaat tgatgcctgt ttctcctgag gaatgccaat
      541 tccctgatct tttacttcaa taatcacgct acccttttgg tttgaaatgg tgacaattac
      601 cagttttcct tcaccatatt tgattgcatt gtcaactaaa ttcgaaaccg cccgataaat
      661 ccaacgatct cgtccatgaa tcagggttgg ttgatcttca gtaaacgcaa acaaaagatt
      721 cggataattc attttgtagg catcacaaac ttccgcacag atcagtgaaa cgtctacgac
      781 ttccgtgcta agatcctctg agttattgga taatgtcaaa atatcgtcaa tactgtcggt
      841 catccgatct aaaagcatta aatttccttg atcggcatgt tgccgttcta tttccagatt
      901 cgtacgcaaa atagcgatgg catttttctg ctcgtgggtc agataactat ttaatcgtcg
      961 ataatcctcc aaatgaccat cgaacttatc ctttagctta ttaaaagccg catttaatgc
     1021 ctcgtccttc acagtatttt taggaacttg atcaataaac gttaaatcac gcgtaatcgc
     1081 taagatttga ttactgtaaa ttttgcgtaa aaccaaccaa agaataaacg ttaataacat
     1141 gacggcagaa caaataacca gcaccatcgc tggtaaatat ttaaatagtt cgtcctggta
     1201 agctttatct atcatcgaaa tagtcataaa ttgattgttg gaagatttcg aagcttccat
     1261 ctgcgtctct gcttgaaccg tcttaccctc ttccataaaa taaacatctt cacctaaggt
     1321 agatgcgata ctgattccct ccaaattatt tctcagaaag aagaaactca ctaaaatcac
     1381 gcctgtcacc aacagtgaaa aagccagcat cgttaacgtc attttaaaat ttaatagcat
     1441 agctggtacc cctttgctcg aatggttttt aagacctctt tttcagctgc ttcagccagt
     1501 ttctttttta gacgagccag atgcactcgc agaaccgacg aaaaaggatc aaaatcttca
     1561 tcgtagacat gttcagaaat ttcctcagac gaaacaaccg ctggatgacg ctgagcgatg
     1621 cacagtaaga tatcaaattc cttgggtttc aggaccacct ctgtcttttg atagctgacc
     1681 cttcgaccta atggatcaat cttcaaagca ccaattgaaa tctgcggcag cgcacgtcca
     1741 tgataacgac gaataaccgc gcgtattcgt gcttttaatt ctagtaactc aaagggtttg
     1801 actaaataat catctgctcc aaaatccaag ccttttgctc gttcttcaat ctcatcacgt
     1861 gcagtaataa tgataactgg actttcgact tcttcagcac ggagactttg taaaacagat
     1921 aatccatctt ttttaggcag atttaaatct agtagtatcc catcgtatga attgatgaag
     1981 gccttctctt ctcctgactc gccatcataa gctacatcta cagaaatatt ctcctgctct
     2041 aggccctttt ttacggataa aaccagctca cgattgtcct caaccaccag tacacgcatg
     2101 tatattcact cctcatacgg atttagtaat tacacttatg ctttttagca tagtacacgc
     2161 tcttcttcaa agtccacgtt ctttttgtga agatttgaag aaattcatac attctgttaa
     2221 aaaacacata attaagaatg attttactgt ttgaaaatac ctagaataga aaaagactgg
     2281 aacctttcct ccagtctcac tactttctat ttgcttggtt ttcttgtata tacttttcaa
     2341 agttcgttgg tttatgacca ataagcctcg ttagaatact gaaaattgcc atcaagtcat
     2401 taagtaccac atgtaagaaa atctctctaa agaaaaaccc cttcgtttta actcacttac
     2461 tattttagaa ttcgttcccc taataatgca gcatgatttt acgctttatg tataaaaaaa
     2521 gactgcaagc aattttgctt cacagtctga tttcttatga tagtgagctt gttaacactt
     2581 ccaccggtgt agcagtttct ttcaatacgg catttaattc ttcaatattt ttacgccctt
     2641 gcgtattcat ccactccatc gctgcttctt ttccatcaac gatatagctt tctacaccat
     2701 ctgcccaagt cgcacggccg caaagtacac cattgaaggt tgagccggca tttttcgcga
     2761 agcgcagtgt ttcttggaac aactctgctt ttacaccggc gcttaggaaa ataaatggca
     2821 ggttagtggc ttcagcctgt tccttgaaga agttagccgc ttgtgccttt gtgtacacga
     2881 cttcgtctgt cccatagcct tccacataat tcatattgac gggtacttcc acctttaaca
     2941 catctacgcc ataacgtgga tcagaaaatt ccttcatcat atcgttgact ttatgaggtt
     3001 tcttaatggc gtattctttc gaagtcgcat cagagatgtt cgcgtcataa gaaacaagct
     3061 ccaagaagaa gggtaagttt tcagcctgac attctgagcc gattcgttca ataaaggcat
     3121 gtttttgatt attgatttct tctgcttcat cgacatcata gtaaagtagg aacttacaag
     3181 catcggcgcc tgcttctttt aaacgtttaa ctgaccagat cgataataaa tctggtaaac
     3241 ggccgggagt tgacgcatcg taaccagttt tttcataagc taacagcaag ccggcatttt
     3301 ttgctctaac gtcagctgct ggcaagccat attcgggatc taataaaatc gatgaggcat
     3361 atttcgttaa ttgtgaagaa acaatttctt taaattcaac gatttctgat tcttctgctt
     3421 cgctgccctt gaattttgtg atcatcttct tcaaagcccc gcgttgatca attgctaagg
     3481 ccccaatcac tccatcttca ttgcatagtt gcttgatata ttgacgtttt tcttcgttca
     3541 tttccgtatc ctcctacact ttctccacta agatttgatc atatagtttt tgataattgg
     3601 ctaattggac atagccagtc gtcgcttcct gagcattcag cattcctaaa acattaccct
     3661 gttttaacac ggcttcatcc gaaagacctt gttgaatcgc agcggtcaat ccggcaacgg
     3721 tcgcatcacc agagccaact ggattgacga catcaatctt tggaattgtc acgcaataaa
     3781 aagtctcgtg atgtttggca aaggctccgt cgccccccat tgaaaccact atccattcaa
     3841 tttcatcaaa catcgaatct gacaaggcgg cttttaaatc tgataattct gttgagattt
     3901 ctcgtcctag cagaccagcc agctcttcgg tattgggttt tattagctta ggctttacac
     3961 tgcctcgcaa cacttccctt aatgctgtac ctgaacaatc taaaacgaca ggctttggct
     4021 gacaaacttg cagcatttgt cgataaaaat cagcaggcaa tccttctggc aagcttcctg
     4081 aaaaagaaat gatttcggct ttaacagcta atttttcgaa atgttgcaag aacgcccttg
     4141 cttcttgttc agtaataaca ggtccgtttt ctaggatttc cgtctgtttt ccgtcatgta
     4201 aaacagcgat gcaattacgt gtctctccgc tgataggtaa aaagtcatga gcaatctcat
     4261 tttgagtcag gtccttttta atctcttcac ctaaaaaacc gccgattaaa ccactagcaa
     4321 tcacctccgt atctaattgc ttcaagatac gtgtcacatt taggccctta ccgccagctg
     4381 ttttgcgaac atttcttact cgattgactg tgtcgagctt gaaggtttct aatggataag
     4441 aaatatcaat cgatggattc atcgttattg ttaaaatcat ttgtccaccc gcttaatcgt
     4501 ggtattcgcc acgatcccat ttctcgataa attcatcgaa gaaatgcgga tcagactgct
     4561 tgtcactggc tttttcgacg tgatcgattt tggcgatcaa ttttttgttt tcttcagttt
     4621 cttgataatc ggcattgatg aaggcctcta atatgtcaca gatcaaaaat tctcccgtaa
     4681 tccgagcgcc aaagccgatc acattggcat ttagttgttc cttcgcatag atagcagtcg
     4741 tcatatcgcg aaccagcgca ctgcggatac caggtacttt attgacagcg ttgttaatcc
     4801 caacacctgt tccacacaaa caaactccta gatctgcttc tccgctagtc acggcctctc
     4861 cgactttttt tccgtagatc ggataatggg ttctggtaaa gtcataggtc cccatatcca
     4921 acacttcatg tccttttgat tttaagaaat ctgatactgc catttttgta tctgtgacga
     4981 tgtggtcaca gccaattgcg attttcattt cattcatcct tcctaattac gccattttat
     5041 ttaacatgtc gacacgtact tgatgacgac caccatcata atttgcttgt agaaattcct
     5101 taacaatatt caacgccaac tcatcgccaa caatttttga tccaagtgtg atcatccgag
     5161 aattattatg tttacgggtc atatacgcac tgcgctcatc cgaaacttct gctgctacca
     5221 ttcctttaac ctttgttgcc gtgataaagc tgccagcgcc gtagccatca accgcgattc
     5281 ctaaactacc ttcgttttca ttcagatcat ttgcgatcgc caaagttgat tccacaaaat
     5341 cttctgaagc cgtttcactc ttatctacta ctgtaatacc ttctttgatc aattttttct
     5401 tgattaattc ctttaattcc atgccgtcac tgtctgaacc aattactaca cgcatttttg
     5461 tttcctccta ttttttactg aatgatttta gtgaattgct gataataact cttcagctcg
     5521 gctgaaagag tagaatccgt aaataatttg tcgatcttgc gcaaatcata gaaattataa
     5581 aaatcgctat ggtcgaattt gtttgcatct gaaaccaaac atttttctgc cgcattatct
     5641 aaaacaattt tttgcaaggt accttcttca atactgaaat tcgaaacctc cgaacccttc
     5701 aatccattca ctccgacaaa agccttctta ataccgattt tttgaacagc ttcattggca
     5761 attgaaccga caaaggctcc agtcttttcc cgatatagcc caccgatcaa atacagttca
     5821 aatcttttta tttcacttaa tagattgaaa accggcagac tattggtaac gattcggaga
     5881 gatgacggct tgatgtatcg tgtcattaat tcgatcgttg tacccgatcc aagaaaaatc
     5941 gtgtcgtctt cttgattgat atgagctgcc gcatttttag cgatgtattc cttctctttt
     6001 gtatgcagct tctttttttc agtatgagaa agctcatttg cttctataga agtcgacatt
     6061 tccagataat cgattttttg tgcaccgcca tgaacgcgta aaagcttttt cttctgcgcg
     6121 agttcctcta gatctcggcg gatcgtcata tctgaaacat tcagtacttc ctgaataaat
     6181 ttgactgtta tcgtcccata gcgatcacac atcgctaaga tcgaacgctg tcgttctgtt
     6241 ttcagcatat tctgtacctc cacgacctta taataaaaca tttacgaaca tttttcaaca
     6301 catattgttc gtttattttc gtttatgcgt tgacaatgat tttgtaagcg gttatactca
     6361 acttgtaaac atataaataa acataaacga actaataaag gagctgtttt gtgtgaatga
     6421 attctttttc aaagacactg tctatgtttc tgacaagcag tcgcaggaag aagtgttcga
     6481 ggaagtctat caagatctgt taaagaaaga attggtaacc gaagattttt tggagaacct
     6541 tttagaacgg gagcgtaatt atccgacggg attagatctc tcacctgtct ctaaaatatt
     6601 gccagatatc gctatccctc ataccgaaag tgagtttgtg cgaaccaccc gtatcattcc
     6661 aatcaaagta aaaaatgaaa tccctttcta caacatgatc tcgccaagcg atcagatttc
     6721 cgcttcattt cttttcatga tcttgaacga aaatggtgaa gcacaggctg gcatgttggc
     6781 taatatcatg gatttcatta attctgttga cgaagaaaaa ctgttttcgt ttttcaaatt
     6841 agaggacaca gaagcgattt atcatttttt agaagaacat ttttatatat agaagtctct
     6901 gcctaaccgc aggataaaaa ataaacttat tttaagggga gaattattat gatcaaaatt
     6961 ttagcagcat gtggagcagg cgttaattca agccatcaaa tcaaaagcgc tttggaaact
     7021 gaattgaaaa atcgtggcta tcaagtcacc tgtgacgccg ttatgatcaa agatatcaat
     7081 gaagaaatgc tgtctcatta tgacattttc gcacaaattg ccaacaccga cttgggtttt
     7141 gacattaaca tccctgtcgt tgatgcaggt gctattcttt atcgtattcc tgctatggct
     7201 gagcctgttt accaaagtat ggaaagcgca atccaatcat taaacaaata ggactaaaga
     7261 aaggaaaagc atatgaatac aattattgat attgccaata aagtatttac ccccctaatt
     7321 aatttaggtg cggcaccgtt aatgaccatc gtattaactg ttattgcttt attttttaaa
     7381 gtaaaaccgt cacgcgctct tgaaggcggg ctcaaattag ggatcgccct gactgggatc
     7441 ggagctatta ttggaatttt aactactgct ttttcagacg ctatgacagc ttttgttgaa
     7501 cgcacaggta tttcattaaa cattacagat gtcggttggg ccccactagc aaccatcaca
     7561 tgggggtcgc catacacgct ttatttttta ctaattatgg tgatcgtcaa tattgtcatg
     7621 ttagcaatga aaaaaaccaa cacattagat gtggatattt tcgatatttg gcatttagca
     7681 attgttggtt tgttcgcgat cttctgcggt gctaatctgt ttattgcttc tgcattagtt
     7741 attttcattg gtgtattaaa aatttggaac tccgacttaa tgaaaccaac cttcaatgac
     7801 ttgttaaatg cgccagacag caacccgatg accacgactc atatgaacta catgatgaat
     7861 ccgattatta tggttttcga taaaattttc gacaaaatct tcccttggtt ggataaatat
     7921 gatttcgatg ctgccaaatt gaatagtaaa attggcttct ggggatcaaa attcgcgatt
     7981 ggaatttatt tggggatttt tgttggatta ctagcaggac aaacaccaac gcaaattttc
     8041 tcgctagcct ttaccgctgc cgtctgtttg gaattgttct ctttgatagg cgcttggttc
     8101 atcgcagccg tagaaccgtt atcacaaggg attaccgatt ttgccaataa acgactaaaa
     8161 ggacgtacca tcaacatagg tttagactgg cctttcatag caggccgcgc tgaaatctgg
     8221 gctgccgcta acgtattagc accaatcatg ttgctagaag ccatcatctt accaggaaat
     8281 aaattattgc cactaggcgg aatcatcgca atgggtgtta ccccagcact attggtcgta
     8341 actcgtggaa aattaatccg tatgatcgta atcggcgcga tcgaattacc attattctta
     8401 tggtccggca ccttgatcgc cccgttcgtt actcaaacag ctaaagcagt cggagccttt
     8461 ccagccggct tgaacgggtc cgccatgatc tctcatacaa cgatggaagg cccgattgaa
     8521 aaattcctag gttatttagt cggtaatgct tctcaaggac aaatcgaatt tgtgatatac
     8581 gctgccttag cattaggtgc atacctttta atcttcattt ggtacgctcg tcaaatgaaa
     8641 aaacgcaacg ctgcatacgc agctgaaaaa gaagagaatg catctccggc aacggcgcat
     8701 aatatgactt atgcagccga agctaaataa gctaaaaaca ccccgaatgt ctttagactt
     8761 cggggtatta ttttacctat aaaaaataaa tagatgtaaa atccatttca aaaagcatag
     8821 cccatctctt gccacaccca ataaagaaat tatcaacgtt tatgcttttt ttaactcctt
     8881 taagcttgcc tttgttggta ttcctcaacg attctttttg tgttcttgtt tgttttgtgc
     8941 tttttgactt tttgttgttt aattgcgaat tccttgattt cttttttcct gaatcgcttc
     9001 gcatcccgcg gttatgcgtg cgatttcatc attgcttttt ctgatctaac tcgtttcatg
     9061 tcaaagagct tcagctgact tagtgtccat ggttgttttg atatcccttt tcctccttaa
     9121 taaagcggtg attgatactg ctattttttc cttcacataa acataacatc ccaaaaaaat
     9181 cgactatctt ttttcaaatg gtgtattcaa gtattcggag gaattagtaa tgatgcaccc
     9241 taaaaataac ataattcgct taaattcaag gctaccaaaa aactaccaag ttctaattat
     9301 ctagagtttg gcagtttctc atttacacaa catatttttc ttctttttcg attttttcat
     9361 agtgcttttg ttcatcatca tccgttataa ctactgttac atcattcaga ttataatata
     9421 ctgaaaaatc tcttttccca attttcgaat gatcaatcaa taaaaagcgt tcaacagaat
     9481 tattcagagc gatctgttga gtttggccct cttcaaaagt cgaagtcata attttctgtt
     9541 catttaaagc attacagctg aaaaatgctt tatgaaaatt catatcctgt agcgatttat
     9601 tcgtgatttc accaacaaag cattgagtcg tttcccgcat ctcgccacct aatagataga
     9661 tttttcgatt cccttttcgc ttatttaatt cttcaaaaac aggcaaacag tttgtaacaa
     9721 tccgcagtga atccccttga atgatttttg acatgagctc gattgtagtt cctggtccaa
     9781 gaaatatcgt atcaccatca ttgatcattt cattcgcctt ttccacaatc cgctgctttt
     9841 cttcttgcag cataatctgt ttttctctat gagaaagctc cacatgccga taaaaatcct
     9901 tcaatttcgc tccgccatgg acgcgttcta ataatccttg tttctccatt tcgtccaaat
     9961 cacgtcggac tgtcatatcc gaaaccgaaa acttctccat aatatcgctg actcgtactg
    10021 taccgttaac ctctaataac gacatgatcg cttggaatcg atccgttttt aacatttcat
    10081 cgcctctttt ctttttaaga ctatcaaatt cttaagaaaa gtgctagaac aagttttctt
    10141 aagaagcctt ctgcaggaag gaaagcaaaa tttttcagaa tttgcaagct gctattctaa
    10201 aaaataaaac cgaacgagca tgactgtcat agtgacagcc ttgttcattc gacttttatc
    10261 tttaataaat cattttattt aatctgaagt tattgtgcaa gttcaaaaac aaagtagtta
    10321 atgtctcctc gatatttcga aatcgaatac tcaacgggca gacgttctag cgaaaaaccc
    10381 tgtgtatgga aatagaacat tggatctcct tcttccacat ctaacaaatc gctgatcgta
    10441 tcatccgctt tgattacttc tagttttctg gtgaccgttt taattggata acccatttct
    10501 tcaaaaactt gatacatttt ttcttttctg atttgtcaaa ttaagctaga caatatgtct
    10561 ttatctatgc agctcaaaaa tacttccaat ggtgttcgat aatttaatga ttttcttgga
    10621 atatggtttc ttttcgaggc aacggattga ataaaggttt catccatgtc cctaaagtcc
    10681 attgttttag gtaagccatc tcgtctaagc aagccgtttg agtgctcgtg ctcatttagc
    10741 cctctttgcg aaggtgttcc tggatctgcg aaaaagatgg caatatcgtt cgagttactt
    10801 atagatcgcc agttggaaaa ttcttttcca caatcaaatg tcatggattt aaacaagtgt
    10861 ttaggcatgg atttgaacca atcatttaac ctgttttcga tatcttcggc tcgtctgcct
    10921 gtcggtttca aagtaataat aaccttggat aaacactcaa ccaatgtaat aacagcgctc
    10981 ttgtgtttaa ctcccacaat ggtgtcgcct tcgagatgac caaattcttt agtcaggtgt
    11041 ggatagagac tgttccgttc atgaatcgtt ctgcgaaagg cctgttttcc acgcttttct
    11101 tgatgattat tcggtttacg tttccccttc ataggtaacg actgtgtgtc aaaaaggcct
    11161 tttttaaaca tccgatagag tgtacgaaca gaacaatcaa ttggaaactc gccacggccg
    11221 ataatcacat cgggtgacca cccttgagct accttttctt gaacatatgt tgattgttca
    11281 tcagataaaa cggttgaacg tcgtccacaa cgagatttat tttgtttgta ttgacggtaa
    11341 taatctagca cttttttcct tgtttgagaa acgaataaac attataaatg gtttgtctag
    11401 agcgatttaa actttgtgcg acaagagcga caggtttctc gatattgaaa taagactcta
    11461 tgattactag ttcgtctgtt gtaagatggg tgtaggtcat ttgtactcac tccttattaa
    11521 ttctttggtc ggaactattt tgagtgtagc acaaatgatc tttttagttg tctagcttaa
    11581 ttttacaatc gacgcctcaa aaagaatacc cgaaatttaa acagcaaaaa aagatagcgc
    11641 ttaccattgt gtttaagata aaacgctagc taagttggct agcgctttat taatctaagc
    11701 aagctctaaa attgcttcaa tacaaaatca actgctcctt taggatcacg tgttaaattg
    11761 atatattctg cacctttagt tgcaattact tttatcccta atttgtctgc atctttacgg
    11821 agatcatcca aataggaatt cacttgaggt gctagaacga ttaaatcaaa atttttcatc
    11881 atatcataat gagaaccata agctcctgct ttagcagaaa gattaacacc tgtctctttt
    11941 gcgccctctt ctaatgcatt agccaacatt gcactcgttc cggcaccagc acaaagtact
    12001 aaaacgcgct ttccttcacc aaccgatgct tttgcttcat caactataac tgccgcagtt
    12061 gcaccaactt cttggatttc ttcgaccaat tcttctttta cttcttctgc agcaatgttt
    12121 tttaactctt gttctactaa ttcagaatca taggccttaa caaaaggtaa ataaataatc
    12181 gcatcgacaa caatcaatac taccgctaat acaatcgcca acacactaaa cccggttcct
    12241 aaaaccaagc caatcggtgc gggcgtcgcc caaggcaata cggatataaa gctattcatc
    12301 cccaagacat cgacaaaaag tttgaatatc catacgttag cgattggtgc taataaaaat
    12361 ggaattagaa agtacggatt caaaataata ggagtagcaa atagtaacgg ttcatttacc
    12421 gcaaacgaaa ctggaataaa tgaagcttta ccaactgctt tcaattgttt tgatttagcg
    12481 aacaacatga acagaaaagg aacaactagt gtcgctccag taccacccat cgttccaaca
    12541 aagtttccta gaccaactgt taatactttt gaggcttgtg caccgttttt aaataattca
    12601 aagttagttt ctacgttcgc ataaataatt gcagcaatcg ctggttcaac aatcgatggt
    12661 ccatgaacac caacaaacca gaacattgcc atggctcccc aaatgattgc aataccgaca
    12721 tatccatcag cagcactaaa taatggctgc aataattgaa ttacacctgc tgcaaatgat
    12781 gttccaaaaa attgacgaac tcccgtatca atcaaaacgg caataaatac tgcaattgaa
    12841 aaggggataa tatctctaaa catctgtgtg attgttccgg gaacttcttt tggcatacgg
    12901 atcgtcaaat ctcttttaac acagaattta tacacattta cagtaacaaa cgcagaaacg
    12961 aaagctgcta ataatccctt tgtccctaaa taatcagtag ctatcccacc atcaatttga
    13021 tcagctgcca tcattacaaa accacaaatt gacgctaaca taacagaaac ttcattaatc
    13081 tgcttacctg aatcagataa ttgacggttc attgaattag ctaaacatcg agctgttgta
    13141 gcaacaacca aaagaccaac taatcccatt gaataaccat aaactttcca aagccacgtc
    13201 gagaatgcct ctggtaacgt cacgttaaat acttctggaa cagccgcaat cataataaaa
    13261 atacttgaaa atagaatagc tggcatagca gttaaaaagc catcgcgaat tgcccctaaa
    13321 taaatattac gtgagatttt ttcaaaaaac ggttttccct tctcaatttg cttaataatc
    13381 gtattcattt ttcttctcct atgctttagc aaatttttct ttgtacaatt caacaaaatc
    13441 attgataagt tctcttaata gaatcgttgt cattaaatgg tcttgcccat gaataaaaat
    13501 tacgcctact tccatttcgt tccctgacgc ttcttcagct aaacatttcg tttgtgagtt
    13561 atgcgctaag gttaaatttt catcagcatc cgctaataat cgttcaacat tatcaaaatt
    13621 accttctctc acttccttca acactgttaa taatgaactt ctggcatctc ctgcataagc
    13681 aacaatttca aatcctaata attgcaattc tttcctatcc atttccttgc cctcctaact
    13741 atttttatat tatcgttttt gtttcgctaa caaatttata ccaatatgca gactctttcg
    13801 gatatctttt ttgtgtatcg aaatctacat agaatagtcc ataacgctta gtgtatccat
    13861 taacccaaga gaaaacatcc attaatgacc aaatgaaata cccctttaca ttaactccca
    13921 ttttgatagc atcggaaatc gcattaaaat gctggcgtaa ataatcgatt cgatcatcat
    13981 ctagaataat cccatcttca aaattatcct tataccctag cccgttttca gtaatgtata
    14041 ttttattata atttggatat tcgtcttgga ttcgtacaag taaatcatat aacccttttg
    14101 gataaatgat ccaatcccaa tcggttttcg gaagatcctc acgcacgatt ctctcgccaa
    14161 tccctttaat gcaatagact tggctacctt tttctccggt agtgttgtag tgaagtgtgc
    14221 tttctccttt gtactcttta acccaatggc taccatagtt attgatacca agaaaatcat
    14281 ttcgatttgc tgcttttttc atctctctaa aatcattaga atcaatatct gcagattgcc
    14341 catttgctgc taaaatctca ttaatatgtg ccattgtttc atctgagtaa taccctaaat
    14401 aagtagcatc taataagaac ttaattgata acgcatcatc taatttcgca gcatgcttat
    14461 cttcttcttt gtcacttgct ggatattttg tctctagcga gtgaacaaca ccaatttccc
    14521 ctttatatcc accatcttta aaaatgttta ctgtctttgc gtgcgcatac atcatgttgt
    14581 gtaaacaagt tacagtcttc tctatatcaa attgaacttc tggaggaaac gatccgatca
    14641 aatattgatt tgaagcaact ggataaatct cattaaatgt actccaataa tgaacctcag
    14701 aaaattcatc gaaacagaac tgtgcatagt ttacgaaatg ttcaacattt tctcgattta
    14761 aaaaatctcc attatcaaat agttctttag gggtatcaaa atgatgaaga gttacaaaag
    14821 gaataatacc atgcttattc aattcagcaa ataccttatg ataatattcc actcctgttt
    14881 ggttcacttc cccatagcct tttggaaaaa tacggctcca tgcaatagac atcctaatac
    14941 tatcaatttt aaattttttg cataattcag catcaactgg atattgatta taaaaatcac
    15001 tagctggatc agcagtatag cgtccctttt ctacaaggaa agtatcccac gctacttgcc
    15061 cttttccacc ttcttttgta gagccttctg cttgataagc tgccgtagct cctcccataa
    15121 taaagtcttc tggtaaagtt ttataattca ttttaatctc tccttgaaaa ttagtttgat
    15181 caatatgcca ataatccata aatcacctca gcataatttt aacatgtata agtaaatttt
    15241 tttacacaac gaaaaaccga cctcgaatat ctattttatt gcgatataca agcacttata
    15301 tcccttgatg taaaataaat ataccattca aaaaccgttt tgtaaacgtt tttttgtaaa
    15361 ataaatttat ctttttacta atttaattta cacgaaacaa gaaaacggac tgattcccac
    15421 taaaatcaat cccacctact aatcattctc caagtggcta tccattatcg attcaatctt
    15481 ctctgttaat ggattaggct ctctgctgat atcagagttt cgtttcctgc tagaaaaagt
    15541 aatttaagtt actaaaagag cagtttcgat ttactttaac aaagaaaagg attattatat
    15601 ccctaggagg atgaacaatg gttactatta gagatattgc aaaaaaatct ggttactcaa
    15661 cggctactat atcaagactt tttaaaggtg atgagtcact ctctattaca tctgatacta
    15721 aaaacaaaat aatcgttaca gccaaatctt taggatatga tctgacaaaa attaagacta
    15781 ctctgtacaa gattgccatt cttttttgga tctcagagga acagttaaaa aaggatgtct
    15841 actatcaaca tgtcaaagat gatttgaaaa attatagtaa aaaagctaat ctagatttga
    15901 ttttcatctc taaaaacgat ggtgtaaaaa gcattcctga cgatattact ggatttatcg
    15961 caataggaca attgacaaaa aaggaattgt actacttaaa tcaaaaaggg tataaggggg
    16021 taatccgagg aataaaccca ttacctgaac acttcgatac tgtagaacct gatttccacg
    16081 aaatggccag acaagcaatt aactacttta ttaatgaagg tttcacaaaa attggattta
    16141 ttggaggaaa tttctttaac ctagaaacgg gtaaagaaga gattgatagc cgtgaatatg
    16201 ccttccgata tcatttagaa aagagcggac tactcaatga aaagtatatt ttctcccaag
    16261 gatcatttac tgttgacgaa gggtatcaat taactcttaa aatgaaagct gagttaaatg
    16321 aagatatgcc cgaggcatgt ttcataccat ctgacactat agctgccggc gttcttcagg
    16381 gctgccatga attgggtatt aacatcccta aagatatggc agtaataagt attaatgatg
    16441 acgacatcgc aaaattcgtt gcaccaccac ttacaacttt taagatagat atcgaagaga
    16501 tagctaaaac ctgtgtagat ttattaagtg atcaactggt atattcgcgg gacatatcaa
    16561 agaaaacatt aatcggcgca aaattgaccg ttaggaaaag ttttatccct aaagaagagg
    16621 tgtaaaataa gtaattgaaa cttatcttta aaactataat tgtgtccaca tagtagccga
    16681 gcaatcgggt ttatgcctat tatgttttcc ataaataatt tgatggtgat attttgacgt
    16741 aaatacaata tgattttttc atcgccaaat tgtgcgcgct aatttttgtt tatcattcag
    16801 aacatttcct cctttttatc taagtatgag gttggataaa cgcttcatct tagtatcttg
    16861 ggggattttt tatctcgttg ggaatatatc aagcatcaga gcgcgcaaaa agaactggtc
    16921 gaaaaatcaa tctaccagtt caatctcatt cactgtgata ctatgtttta tacagataac
    16981 gccatattat aaaacaagtt gtattccgca aatgggttct attcaactct taaactatct
    17041 ataaaagatt cagccaactc cattagcctc tcgtaattta gttggtgaac agcacttttt
    17101 tcaaaaaatt ttgaaccaat tcctacataa gaggctccat tgttcaaata gttttgtgca
    17161 ttagtctgat ctacccctcc tacagccatc aaaggtaaac ttcctaaagg ggcttgcact
    17221 tgattgaaat attccggtcc aagacagttg gcggggaaca ccttaacaat atcagcaccc
    17281 atctcaaaca ttttgaatat ctctgtgggc gacatggccc caggaattgt tagaaggttc
    17341 tgttgcgaag tttgaaaatc aaacgcgccc tctctgaact ccaaatatct taggctgtta
//
LOCUS       DAHHZJ010000044        16913 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.045, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000044 DAHHZJ010000000
VERSION     DAHHZJ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.045"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..1152
                     /locus_tag="NBL02_001217"
     CDS             <1..1152
                     /locus_tag="NBL02_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HCD2571914.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="NBL02_001218"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="NBL02_001218"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCD2571915.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="NBL02_001219"
     CDS             2728..3003
                     /locus_tag="NBL02_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HCD2571916.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="NBL02_001220"
     CDS             3009..3776
                     /locus_tag="NBL02_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HCD2571917.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="NBL02_001221"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="NBL02_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HCD2571918.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="NBL02_001222"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="NBL02_001222"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HCD2571919.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="NBL02_001223"
     CDS             7920..8222
                     /locus_tag="NBL02_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HCD2571920.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="NBL02_001224"
     CDS             8225..9388
                     /locus_tag="NBL02_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HCD2571921.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="NBL02_001225"
     CDS             9414..12842
                     /locus_tag="NBL02_001225"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HCD2571922.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="NBL02_001226"
     CDS             12863..13987
                     /locus_tag="NBL02_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571923.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13977..14414
                     /locus_tag="NBL02_001227"
     CDS             13977..14414
                     /locus_tag="NBL02_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCD2571924.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="NBL02_001228"
     CDS             14398..14736
                     /locus_tag="NBL02_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HCD2571925.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="NBL02_001229"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="NBL02_001229"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HCD2571926.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="NBL02_001230"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="NBL02_001230"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCD2571927.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..>16913
                     /locus_tag="NBL02_001231"
     CDS             15893..>16913
                     /locus_tag="NBL02_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HCD2571928.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEAL"
BASE COUNT         5723 a         2930 c         3735 g         4525 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttg
//
LOCUS       DAHHZJ010000045        16902 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.046, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000045 DAHHZJ010000000
VERSION     DAHHZJ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.046"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            387..1721
                     /gene="dnaA"
                     /locus_tag="NBL02_001232"
     CDS             387..1721
                     /gene="dnaA"
                     /locus_tag="NBL02_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCD2571929.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            1919..3049
                     /gene="dnaN"
                     /locus_tag="NBL02_001233"
     CDS             1919..3049
                     /gene="dnaN"
                     /locus_tag="NBL02_001233"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCD2571930.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            3278..3520
                     /gene="yaaA"
                     /locus_tag="NBL02_001234"
     CDS             3278..3520
                     /gene="yaaA"
                     /locus_tag="NBL02_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HCD2571931.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            3507..4631
                     /gene="recF"
                     /locus_tag="NBL02_001235"
     CDS             3507..4631
                     /gene="recF"
                     /locus_tag="NBL02_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCD2571932.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            4628..6574
                     /gene="gyrB"
                     /locus_tag="NBL02_001236"
     CDS             4628..6574
                     /gene="gyrB"
                     /locus_tag="NBL02_001236"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCD2571933.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            6594..9065
                     /gene="gyrA"
                     /locus_tag="NBL02_001237"
     CDS             6594..9065
                     /gene="gyrA"
                     /locus_tag="NBL02_001237"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HCD2571934.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            9301..9600
                     /locus_tag="NBL02_001238"
     CDS             9301..9600
                     /locus_tag="NBL02_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCD2571935.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            9650..10162
                     /gene="ssb"
                     /locus_tag="NBL02_001239"
     CDS             9650..10162
                     /gene="ssb"
                     /locus_tag="NBL02_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCD2571936.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            10188..10424
                     /locus_tag="NBL02_001240"
     CDS             10188..10424
                     /locus_tag="NBL02_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCD2571937.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            10567..12540
                     /locus_tag="NBL02_001241"
     CDS             10567..12540
                     /locus_tag="NBL02_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HCD2571938.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            12546..12998
                     /gene="rplI"
                     /locus_tag="NBL02_001242"
     CDS             12546..12998
                     /gene="rplI"
                     /locus_tag="NBL02_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCD2571939.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            13272..14639
                     /gene="dnaB"
                     /locus_tag="NBL02_001243"
     CDS             13272..14639
                     /gene="dnaB"
                     /locus_tag="NBL02_001243"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCD2571940.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            15419..16711
                     /locus_tag="NBL02_001244"
     CDS             15419..16711
                     /locus_tag="NBL02_001244"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCD2571941.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         5619 a         3051 c         3616 g         4616 t
ORIGIN      
        1 tatgggaatc ctatgtggaa aaagttatgc acagggatta tccacagata ataaaaggct
       61 ggttttacac agaaaacaca cgtgtaaaaa aagtaatcca caatcaggcg atttcctgtg
      121 tattccgtct cctaactatt ggtatatctg tattttttat ccacagacgc aagaggaaat
      181 ctattggttt ttacgagttt tccacctgtg cattagtggt gtggaaaact ttttttgaca
      241 actgtgtttt tctctttttt ctttttgtcg attgtgtgga taattgtaat taaaaaattt
      301 ttttcttcgg tttctgtgga aaactattga aaaaaatgct aaattagtac tagttattca
      361 ctttttaatg aaggaggagg ccttatatgg tatccctcga tgctttatgg aatgaattaa
      421 aagcaacata ccaaaaggat ttatcgcctg ctagctataa tacatggatc gaaacagcga
      481 accctcgaac ccttgatcaa aaccagctcg ttgttgaggt tcctagcaag attcataaag
      541 aatattggga aaaaaacctg gcgacaaaaa tcgttgaagt cggttatatg ttatcaggaa
      601 atgagattat ccctcgcttt attactggcg aagaagcaga gcaagaagaa gttatagaag
      661 aaaaaaatcc aaaagttgtg gcacctagtc cgctaaaaaa agccatgctg aaccctaaat
      721 atacctttga tacgtttgtc attggtaaag gaaaccagat ggctcatgca gcagcgctcg
      781 ttgtagcaga agatccaggt tctatttata acccgctctt cttttatgga ggcgtgggac
      841 ttggtaagac tcacttgatg catgcaattg gtcatcaaat gctgcaaagt cagcctaatg
      901 ccaaagtaaa atatgtaagc agtgaaactt ttgctaatga tttcatcaat tcgatccaaa
      961 acaaaacagc agaagagttc cgtcaagaat atcgaaatgt tgatttactg ctagtggatg
     1021 acattcaatt ttttgcagaa aaagaagcta cgcaagaaga gtttttccat actttcaatg
     1081 ccttgtacaa tgaagggaaa caaatcgtac taaccagtga tcggttgcca aacgagattc
     1141 cgaaactgca agaacgcctt gtttctcgct ttgcatgggg gctgtctgtt gatattacac
     1201 cgccggatct tgaaacaaga acagcgattt tacgtaaaaa agcggcggct gaacgtctag
     1261 agatcccaga tgacacacta agctacatcg caggacaaat tgattctaac attcgtgaat
     1321 tagaaggtgc gcttgttcga gtgcaagctt ttgctgcgat gaatagtgaa gatatttcga
     1381 ctagtctagc tgcagacgca ctgaaaacgt taaaatcagg aaaaggacat cctcaattgt
     1441 caatcctgca gatccaagaa gaagtggcaa agtattatca catccaattg aaagatctca
     1501 aaggaaagaa acgcgtcaaa tctattgttg ttcctagaca aatcgctatg tatttggcac
     1561 gtgagctcac agataattct ttaccgaaaa tcggagctga attcggtggc aaggatcata
     1621 caacggttat ccatgctcat gaaaaaatcc agcagctgat ggattctagt gtcagtatgc
     1681 aaaatgaagt atcagaaatc aaaaactttt tactaaatta atttgtcgat tctatcaact
     1741 gaaaatagca tgtggataaa agaaaaaaac gttcaaaagt tatacacact ttttccacag
     1801 ctgttttttc gtttctctct tacgtttttt gagttttcca cagaatcaac atgccctatt
     1861 acttttatta ttctttttaa ctaataataa ataaacaaac attcaaagga gatacatcat
     1921 gaaagttact ttaaaccgag ctagctttat gcaggaattg caaactgttc aacgagctat
     1981 ttcaagcaaa accacgatcc ctattttgac aggtgtaaaa atcacactga cacaagaagg
     2041 tttgactttg acggggagca acgctgatat atcaattgaa acttttttgt ctgttgaaaa
     2101 cgaaaaagca aatatgcaaa tcgaatctac tggttccatt gttttacaag cacgtttctt
     2161 tagcgaaatc attcggagac ttcctgaaga aacatttact ttagaagttt tagaaaataa
     2221 acaagtagcg atcacttctg gaaaagcgaa ttttatcgta aatggattag atgcagataa
     2281 ctatcctcat cttcctgttg tcgaaagcca taaccagatg aaattacctg tacacgtatt
     2341 gactaaacta atcaacgaaa cagtttttgc tgtctcacaa catgagagtc gtccaatctt
     2401 gacaggtgtg catttcattt tatctgataa ttctttatta gctgtagcta ctgattctca
     2461 ccgtctaagt caacgcgtga ttccagtaga acaagcggct gatcattttg atattgttat
     2521 tcctggaaaa agtttgatcg aattatctcg ctcattaaca aatgaagaag aaatcgtcga
     2581 aatcagcatt atggaaaacc aagtgttgtt caaaacagaa acgatgtatt tctattcccg
     2641 tttgctagaa ggaaactatc cagataccaa tcgtttgatt ccttcaagtt ttaatacaga
     2701 ggttgaattt tctgttccta gctttttagc ggcgatcgaa cgggcttctt tactttctca
     2761 tgaaggccgt aacaacatcg ttcgtttatc tattcgtcca gatgcggttg ttttatacgg
     2821 aaattcgcca gaaatcggga aagtagaaga aagcttgagt tatacagcaa gcagtggcga
     2881 tccattagat atttctttca atccggatta tatgaaagca gcgttgcgtg catttggaga
     2941 tatgagcatc aaagtgaaat ttatctctgc tattcgtcca tttacattgg aaccaacgga
     3001 agatggcgtt caatttattc agctgattac tcctgtacgt acaaattaac aatgattttc
     3061 aatgttcttg aaaatttaat tgaaaagaaa tagaaaaaat aaaattttaa acagaaggcc
     3121 ctaaaatctc ttttaaggct ttctgtttat ttttgtttaa atcacctttt ttagattaaa
     3181 tcgtcttata tggaaaaata tatggataaa tttgttttat tgcgaaaaaa aaggtataat
     3241 agagtatact tttaataatg aaaatgaggg atgggcattg aaacaacaag tcattctaaa
     3301 tacggaattc atgacgttgg gccaaatgtt gaaagaagtc acagttatcg gtagtggcgg
     3361 acaagcaaaa tggtatttgg cagaaaacac tgtgttagtc gatggcgaac cagaaaatcg
     3421 tcgtggacgc aaactttatc ccggcatgat ggttgaagtt ccagaagtag gaactttttt
     3481 tatggtaaaa aaaggaaacg acgataatga ggctgaataa gctgtattta aaaaattatc
     3541 gaaattacga agagctgaac atcgaatttt ccaagaacct cgtcatcttt ttaggagaaa
     3601 atgcgcaagg aaaaacgaat cttttggaaa gtatctacgt attagctatg acgcgaagtc
     3661 atcggacaag tagtgagcag gaattgattg gctggactga cgatcaggcg atgattcaag
     3721 gagagatcac aaaaggttca tccactcttc cattagaaat cttattatct aaaaaaggcc
     3781 gaaaaaccaa gatcaaccat atcgaacaga aaaagttaag tacttacgtg ggacaaatga
     3841 atgtcatttt atttgcacca gaagatcttt ctttagtaaa aggcagcccg cagatcaggc
     3901 ggaagttttt agatatggaa ttaggccaga tcaatccaat ctatctttat gatcttgtcc
     3961 aataccaagc aattttgaaa caaagaaacc agtacttgaa gcaattgaat gaaaaaaaac
     4021 aaactgattt gctttatttg gatattttgt ccgaacaact agcagcgttt ggcagcaaag
     4081 tattgaaagc aagagcacag ttcgtcaagc gattggagtt ttgggctaat tctttgcatc
     4141 agcaaatcac ccatcaaaaa gagcagctgg aaatcgagta tctcaccgca gtcgattctt
     4201 tagaaacaca tacgcaagag caaatccaag aacaatttct cgcacttttg aaccaaaata
     4261 aaaagaaaga cctttttcgg ggaacaacga cagttggtcc tcatcgtgac gatctttcct
     4321 tttttattaa tcagaaaaat gtgcagactt atggatcgca aggacagcaa agaacaactg
     4381 cactaagtgt caaattagca gaaatcgatt tgattaaaga agagacaggg gaatatccaa
     4441 tccttttgtt agatgatgtg atgagcgagc tggatgataa tcgtcaactc catctgctag
     4501 aaacaatcga aggaaaagtt caaacgttct taacaacaac gacacttgac catgtaaaaa
     4561 ataaaatgac tgttgaacct gagatttttt atgttcaaca agggaaacta aaaggagaag
     4621 aaactgaatg acagaagaaa gaagtttagt agaacgcgct aaagagtatg atgccagtca
     4681 gattcaagta cttgaaggac ttgaagccgt tcgtaaacga cctggcatgt atatcggttc
     4741 gactagttct gagggtctgc atcaccttgt ttgggaaatt gtcgataatt cgatcgatga
     4801 agtattagca ggttttgcaa cgaaaatcca tgtgattatt gaaaaagaca acagtatcac
     4861 cgttattgac gacggtcggg ggatacctgt agatatccag gcaaaaactg gacgtcctgc
     4921 agttgaaacg gtctttactg ttttacatgc tggaggaaaa ttcggcggtg gtgggtacaa
     4981 agtttctggt ggacttcacg gagtaggatc ttctgttgta aacgcgcttt ctacacagct
     5041 tgatgtcaaa gtatacaaag aaggaaatgt gtactatcaa gaatatcgtc gtggagctgt
     5101 agtggacgac ttaaagatta tcgaacaaac ggatcgccat ggaacaacgg ttcactttac
     5161 gccagaccct gaaattttta cagagacaac tgagtttgat ttttcaaaat tggctacccg
     5221 gatccgtgaa cttgctttct tgaaccgcgg gatgcgtata tcgattgaag acaaacgaga
     5281 agaagaacca gtcatcaatg aataccatta tgacggcggg atcaaaagtt atgtcgaata
     5341 cttgaatgca aacaaaaccg tgatttttcc agaacctgta tatttggaag gtgaacagca
     5401 agatatcgca gttgaagtct ctatgcaata tacagacggc taccattcca atatcatgag
     5461 ttttgccaac aatatccata cgtatgaagg tggaacacat gaatcaggat tcaagactgc
     5521 tttaactcgt gtgataaatg attatgcccg aaaacaaaag ctgatgaaag aaaatgacga
     5581 caacttgacc ggtgaagacg tgcgagaagg attgacagct gttatttcta tcaagcatcc
     5641 tgaccctcaa ttcgaaggac aaacaaaaac aaaattgggc aattccgaag tacggacagt
     5701 cactgatcgg ttatttagcg aacatttcat gaaattcttg ctggaaaacc caagtgtggg
     5761 ccggcaaatt gttgaaaaag gattactagc ttccagagct cgtctagcag cgaaacgtgc
     5821 gcgtgaagta acgagacgta aaggtgcttt agagattagt aatctgccag gaaaattagc
     5881 ggactgctct agcaacgatc ctgaaaaatg cgaattgttc atcgtcgaag gagattcggc
     5941 cggcggatca gctaagcaag gtcgtaatcg agagtttcaa gccatcttgc ctattcgtgg
     6001 gaaaatcttg aatgttgaaa aagcaagcat ggacaagatc ttggcaaatg aagagatccg
     6061 gtctttattt actgctatgg gtacagggtt tggagaagat tttgatgtat cgaaagctcg
     6121 ctaccacaaa ttagttatca tgactgatgc cgatgtcgac ggcgcgcata tccgtacatt
     6181 gctgctgact cttttttatc gttacatgcg tcctattgtt gaagctggat acgtctatat
     6241 tgctcagcca cctttatacg gggtaaaaca agggaagaat attacgtatg tacaaccagg
     6301 aaaaaatgca gaagaagagt tgaaacaagt cgttgcttca cttcctgcct caccaaaacc
     6361 aagcgttcag cgttataaag gacttggaga aatggatgat catcaactat gggaaacaac
     6421 tatggaccca agcaatcgga tgatggcccg cgtcagtgtg gacgatgcta ttgcagcaga
     6481 tcagatattt gaaatgttga tgggggatcg agtagaaccc cgtcgtgcat tcatcgagga
     6541 aaatgcacac tatgtgaaga acttagatat ttaatcatga aggaggaaaa agaatgagtg
     6601 aagaaatcag agaaaacatc catgatgtca atctaaccag cgaaatgaaa gactccttca
     6661 tcgattatgc gatgagtgtt attgtcgcac gtgcacttcc tgatgtcaga gatggtctga
     6721 aacccgttca tcgccgtatt ttatacggca tgaacgaatt aggtgtaaca ccggataaag
     6781 cccataaaaa atctgccaga atcgtcggag atgtcatggg taaataccat cctcatggag
     6841 acattgcgat ctatgagtca atggtacgga tggcacagcc attcagctac cgttatatgc
     6901 tagtggatgg tcatggaaac tttggttctg ttgacggtga cggtgccgcc gctatgcgtt
     6961 ataccgaagc acggatgagc aaaatcgcta cagaaatgct gcgtgatatc aataagaaca
     7021 ctgttgattt ccaaagcaac tatgatgata cagaaaaaga accagttgta ctacctgctc
     7081 gcttcccgaa tcttttagtg aacggaacaa cggggatcgc cgtaggtatg gccacgaata
     7141 tcccacctca taacttgtct gaggtagtag cggctatcga tttgctgatg gataatcctg
     7201 atgtgacgac caatgaattg atggaagtat tacctggacc agattttcca acaggtggtt
     7261 tggttatggg gaaatcagga atccgaagag cgtatgaaac aggtaagggt tctattaccg
     7321 tacgtgcaaa agttgagatt actgagatgc caaatggtaa agaacggatc atcgtgacag
     7381 aattgcctta tatggtcaat aaagcaaaat tgatcgaacg gatttctgaa ctgcacagag
     7441 acaagcgtat cgaagggatc acagatttgc gggacgaatc ttcccgtgaa ggtatgcgga
     7501 tcgtcattga catccgtcgt gacgccagcg catccgttat cttgaataac ttatataaat
     7561 tgacatcctt gcaaaattca tttggattca atatgttggc gatcgaaaaa ggtgtgccga
     7621 aagttttgag cttgaaacaa atccttgaaa actatatcga acatcaacgc gaagtcatca
     7681 cacgacggac ggtctttgaa aaagaaaaag cagaagcaag agcacacatc ttagaaggtt
     7741 tgcgtattgc tttggatcat atcgatgaaa tcattgcgat tatccgcggg tctaaatcag
     7801 atgacgaggc aaaagcaaca atgatcgaac ggttcgattt atccgatcgc cagtctcaag
     7861 ctattttaga tatgcgttta cgccgattga ccggtttgga acgggaaaaa atcgaaaatg
     7921 aataccaaga actcttaaaa ctgattgaag atttaactga cattctggca agaccagaac
     7981 gtgttacaga aatcatcaag aatgaattag cagacttgaa tcaacgattt ggtgataaac
     8041 gacgcacaga attactagtc ggtgaggtct taagtctgga agatgaagat ttaatcgaag
     8101 aagaagaaat cgttattaca ttgacaaaca atggttacat caaacgtttg gcaaataatg
     8161 aattcagagc tcaacgaagg ggtggacgtg gtgtccaagg aatgggtatc cacaatgacg
     8221 acttcgtcaa aaatctggtt tcttgttcta cccatgatac attgttattc tttacgaata
     8281 acggaaaagt gtatcgggca aaaggatacg aaattccaga atacgggcgt acagccaaag
     8341 gaatcccaat catcaattta ctaggtatcg attcgagtga aagcatccag gcaatcattg
     8401 cggtgacggg agaagcagaa gaaggccact atctcttctt tacgacgcgt caaggtacag
     8461 taaaacgtac ttctgtcact gcttttgcta acatccgaag taatggtctg atcgcaattg
     8521 gtttgaaaga agacgatgag ctcgtcaatg tgctactgac agatggtcaa tctaatatca
     8581 ttattggaac acataacggc tactctgtca ctttcaaaga agaagcagtc cgcgatatgg
     8641 gacgtacagc ttccggggtc agagggatac gcctgcgtga ggaagattat gtcgttgggg
     8701 cagccttaat gaaggaaaat caagaagtat tagtgatcac agaaaaaggt tacggaaaac
     8761 ggacaaaagt atcggaatat ccagtgaaag gccgcggagg taaagggatc aaaacggcga
     8821 acatcactga aaaaaatggt ccactagctg gattgaccac tgtttctgga gatgaagaca
     8881 tcatgctgat tacagataaa ggtgtcatta tccgtttcaa cgtatcgact gtttctcaaa
     8941 ctggacgttc aacattaggc gtacgtttga tgaagatgga agctgatacg aaagttgtca
     9001 cgatggcagc agtcgaacca gaagctgcag aacaaacagt tgaagaacaa ggatcagaag
     9061 aataaccatc aagactaagt gtgtaagcta gcaagaataa ccgattaaaa aatctggtta
     9121 ttcctgctgg cttttttgat tagttgaatt tctcttaaaa tattcgaaac tgttcattct
     9181 gtattgcttt tgaccaaaat agagtgtata attagagctt gtgagtagtc taattaatta
     9241 gataactctc cttgctctta ggaacctaag agccgaaagt ccacagggag gtgaatatca
     9301 atggaaaaca cgaaatatga aatcatgtac attattcgtc caaacattga tgaagaagca
     9361 aaaactgctt taatcgaacg tttcgatgca atcttgaaag ataacggagc agaagttatc
     9421 gaatcaaaag attgggaaaa acgccgtttc gcttatgaaa tgaacggtta ccgtgaaggt
     9481 atctatcaca tcgttaaagt atcttctcca tcatcagcaa atgctgtcaa tgaatttgac
     9541 cgtcttgcta aaatcaacga cgatatcatt cgtcacatga ttgttaaaga agaagcttaa
     9601 ttgttgtttc atgtgaaaca atagaaaacg atgagaggag atgactattt tgattaataa
     9661 tgttgtatta gttggacgtc tgacgaaaga tccagatttg cgttacacag caagtggtac
     9721 ggcagtcgca acatttactt tggctgtgaa tcgtaatttt acaaaccaaa gcggcaatcg
     9781 cgaggctgat tttatcaact gtgtgatttg gcgcaaatca gcagaaactt tagcaaacta
     9841 tgctcgtaaa ggaacacttt taggtgttac tggacgtatc caaactcgtt cttatgataa
     9901 tcagcaagga caacgtgtat acgtgacaga agtagtagct gacaacttcc aattgctgga
     9961 aagtcgttct gcatctgaaa atcgccaaca aagcggcgga ttccaaagtt ctggtcaatc
    10021 agctggcgga tttggcggaa acaataactc taatcaaaca tctcaatcat ccaacggtat
    10081 gcctgatttc gaccgtgata cttctgaccc attcggctca agttcaacga tcgacatttc
    10141 agacgatgat ttaccattct aaacgaattt gataggaggg aaatagaatg gcacaacaaa
    10201 gaagaggcgg acgtaaacgt cgtaaagtag actacattgc tgctaaccat attgaatata
    10261 tcgattataa agatatcgaa ttactaaaaa gatttatttc agaacgcggt aaaatcttgc
    10321 cacgtcgtgt aactggtaca ggcgctaaaa accaacgtaa aattactgtt gcaatcaaac
    10381 gcgctcgtat catgggcttg ctacctttcg ttagcgaaga ctaataaatc ctaggaattg
    10441 aatgactgat ccttttgatc agctattcaa ttcctttttt tctgtaaaaa taacctctat
    10501 tcgtttagtc gctcgaatga cgaaagttat ggtaaaatca aattagtcat atatgggagg
    10561 aaaattatgg aaaataaaaa caatcattta tccccgaaac ctctcttcct tatcctgttt
    10621 atattaatcg aagtattggt agtcttaatt ttaccatttc gttttgttag cgcttcggtc
    10681 attctattga tgaatttatg gggtgttcgc tattggttga aaatgaaacg ttggcttatt
    10741 ttaaataaac gtgaaaaaat ccagcaggct tcgactgtag ctgaaaccaa tctagcttat
    10801 gtctcagaag tcatgcctgt aggagtgatt gcttatcagc catcaagcca aaaaatcgaa
    10861 tggctcaatc cattagcatt gaaaatcaaa gatcaaagtg gattaacaga ggaagaattg
    10921 ctagatactt tttttactgc aaaagaaaaa aagaaaaacc aagtctatct agcagataaa
    10981 acatttcagt ttgatatgga tacagtaaag ggtgttgttt attttaatga tattacccaa
    11041 aaccagttgt taaaactcca tcagacgaac agtcagccgg tgattggtat tttatctatt
    11101 gataactatg cagatgccat tgataaatta gatgacaaag agatttctta tttgaactct
    11161 ttcgtcacta cactagtttc tgactggatg aatgaatacc atgtcttttt caaaagaatc
    11221 aacgcagaac ggtttttctt cgtagcacag atgatcgatc tgaaaaagat gatggataaa
    11281 aaatttgatt tgctggatcg ctttcgagca gaagctgaaa aacagagctc gcctatcacc
    11341 cttagtatgg ggattgctta tggctcaaga accctcgatg aaatcggtgg agaagctcag
    11401 aataatttag atgttgcttt agtccgtgga ggcgaccaag ttgtcgtgaa agaggtaaaa
    11461 gaaggagcaa aacctgttta ttatgggggg aaatctgcta agaatgtcaa acgcacgcgt
    11521 gttcgttctc gagcgatgag taccgctttg cgtcggattt ttgatgaaac aggcgatgtg
    11581 tttatcatgg ggcacaagtt tccggatatg gatgctttag gctctgcttt tggtgtcagt
    11641 tgtctggcac gcttcaacca gaaaaaagct tacatcgtta tcaatgagaa cgagatcatt
    11701 ccagatgtag aacggtgtct tgaagaaatc cataagcatc ctgaattaga aatgcaacta
    11761 atctctccag aacgtgccat agagctgaaa aaagaagagg acctcctcgt gatggtggat
    11821 taccataaac cttctctatc gatctctcag ggcctatatg agcaatttga taagattgtg
    11881 atcattgatc accatcgtcg tggggaagag tttccaagta aacctctgct gacttatatt
    11941 gaatcttcag cttcttcagc ttcagagcta gtttctgagc tgatccaata ccaaagcagc
    12001 aaaacaaatc gcttagatcg ttttgaggcc acattattgc ttgcagggat ggttgtagat
    12061 acaaatagtt tcaatgtccg tacaactgca cgaacttttg acgttgccag ctatttaagg
    12121 tcttgtggag cagacgcatc cttagtacaa tatttattga gctcggattt agcgacatac
    12181 ttggagatca gcaatttgat tgctggaagc gactatgtga cttctgatat tgttgtggcg
    12241 gcagggacag acgaaaaaga gtataatagt gttatagcag caaaaacagc agatacattg
    12301 ctttcgatga tcaatatcaa tgcagctttc gtcattacaa agcggacaga cggcttgatt
    12361 gggatcagtg ccagaagtac cggcacgatc aacgtccaaa tcatcatgga atccttaggt
    12421 ggcggaggac acttcacaaa cgcagctact caactggaaa atacgacagt tgaggaaaca
    12481 aaacagaagc tatttgctgc aatcaaagaa aatatgaatc aaatatatga taaggagtaa
    12541 tccaaatgaa agtaattttt ttacaagatg taaaaggaaa aggtaaaaaa ggggacatca
    12601 aagaagttcc agcaggttat gcacaaaatt atctaatcaa aaataacttg gcaaaagaag
    12661 caacgaaaag cagtatcagt cagttagcag gacaaaagaa agcacaagaa aaacacgaag
    12721 ctgaaatctt ggaagaagct aaaaagttaa aagaattctt ggaaaaagaa gatactgtag
    12781 tcgaattgaa ggcgaaagca ggggaagaca gccgtttgtt tggttcgatc ccttcaaaac
    12841 aaattgctga agggttaaac aaacaataca aaatcaaatt agataaacga aaaatcgaat
    12901 tagaaaatcc gatccgttca ctaggctaca caaacgtacc tgttaagctt catcatgagg
    12961 tgacggctac gatcaaagtt catgtcgtac aagaataaca aatcttgata gcgatgttca
    13021 gctggtaagt ataaccagaa ggaaaaaaga caaaccgaaa tttatctgag aaaacagctg
    13081 cagcaatgaa agcaaaagct gcaaatcgat ttcagattga tttcggtttc ttttttggga
    13141 acagcaaaat agcataaaga aaacgagtat aataaaagag aactattttt ttattacact
    13201 ttcggtacga atgatgactt ttctctcatt ttcttgtaaa atgaagcaat tgaagacaag
    13261 gagcgatacg tatgaatgaa gtgtggcagg atcgaatccc accgcaaaat attgaagctg
    13321 aacaagcggt tttaggctct gtttttcttg acgcagaaac gatcattgat gccatggagt
    13381 acatagagcc aaaagacttt tatcgtcgtg gccatcaaat tatttttgag acaatgatcg
    13441 agttaaatga tcgtaatgaa gcgattgacg tggttactgt caaagaccgg ctggaacaag
    13501 ccaatttgtt agaagatgcc ggtggtttaa gttatctctc agacttggca ttagcagttc
    13561 cgacagcagc aaatatcgtc tactatgcga aaatcgttga agaaaaatca ttgctgcgta
    13621 cattgatcca gacagcaaca ggaatcgtta cgaaaggatt cgagcaagga gaagaagttc
    13681 aaacaatttt agatgatgcc gaacgcagta tcttggaggt ttcggaaaaa agaaatcgaa
    13741 gcggcttttt atctattgct gaagtactga actccacgat tgaaaatatc gatcgactag
    13801 ctcaaaacaa tgaagaaatc acaggtctac caactggcta tcaaggtctg gataaaatga
    13861 cagcaggttt ccagcccgaa gaactaatca ttcttgctgc acgtccagcg gtaggtaaaa
    13921 cagcgtttgc attgaatatt gctcaaaatg taggaacaaa aacagatcgt tctgttgcga
    13981 tatttagttt ggaaatgggg gcagaatctc ttgtaaatcg gatgctttgt gcagaaggtt
    14041 cgattgaggc cagccattta cgtacaggtc agctttcgga agaagaatgg tcgaatctga
    14101 tcgttgcgat ggggagtctt tctcgagcaa atatttttat cgatgataca ccagggatca
    14161 aaatctctga aatccgtgcg aaatgtcgga aactagcgca agaaaaaggc aatctaggct
    14221 tgatattgat cgattatttg cagttgattg aaggtaacgg taaagagaac cgtcaacaag
    14281 aagtatcaga tatctctcgc caactgaaaa aactggcgaa agagctgaaa gtacctgtca
    14341 ttgctctttc acagttatct cgtggagtag aacagcgaca agacaaacga ccagtgctaa
    14401 gtgatattcg tgaatccgga tcaattgaac aagatgcgga catcgtagcc ttcttgtatc
    14461 gagatgatta ttatgaaaga gaaggcggag atgacgatga tggaaacgag ccaaaaaatg
    14521 acaacgtcat cgaagtcatc atcgagaaga accgaagcgg tgcacgagga acagttgaac
    14581 tactgtttat caaagaatac aataagtttt cgtcgctttc gccaagagaa gaattttaaa
    14641 gaagaggttg tgagccttga gcaatcccga gagctctcgt aagaagatgc aaggagtaac
    14701 cgcaggaacg actagagtac ttaatttaaa cagatctcca aagggattgt gacaaaagtc
    14761 ttgtcacagt ccctttttcc gaataaacgg tgttcaatca gctgaccctt ggtattagct
    14821 cagcaaacgt caaaatatga ggaactattt tgacgtttgt ttctctaata ctcaggtcaa
    14881 aacgctgatc ttacgacctc ttttccttaa acggtgttct cctgccaact cctcggtcac
    14941 aagtcacaat atcatcaaac atgagaagca gttttttgaa attgctcctt gtgactcaga
    15001 gttaaacggc aggctcacaa cctctttctt aaacggtgtt caaaagcttg catctgcggt
    15061 aatacgccca atcaaacgaa aaatatgaga agctattttt gtttggctgt tcttattact
    15121 tgatgcagga cagctttttc acaacctctg attcacggtg ttccaaaagt agcttcttcg
    15181 aaaaataagt cgaaatttca caaaaatttg agaagcaatt ttcgtacatt ccttcttatt
    15241 tcttgaagct gaccacttct gttacaacct cttttttaat aaccattcta aaaagataaa
    15301 gttcgttttt taagcgtttt tataaaataa aaataaaaat cattcgattt tatttgaaat
    15361 atgtatctat tattgataca atggttcagg attaaataat atagaacgag gtgttcacat
    15421 gtcatctgta gtagtagtag gtacgcaatg gggcgatgaa gggaaaggaa aaatcacaga
    15481 ctttctcagt gaaaatgctg aagtgattgc acggtaccaa ggtggcgaca atgcagggca
    15541 cacaattaag tttaacggtg tgacttataa actgcatttg attccttctg ggatttttta
    15601 taaagacaag atcagtgtga tcggaaacgg cgttgtagtc aatcctaaat cattggttaa
    15661 agaattagct tatttacatg aaaaaggtgt agaaacaagt aatttgcgta tttctgaccg
    15721 cgcgcacgta atcttgcctt atcatatcaa attggaccaa ttacaagaag atgctaaggg
    15781 cgaaaataag attggtacaa cgattaaagg aatcggacct gcctatatgg ataaagcagc
    15841 tcgtgtaggt atccgtattg cagacttatt agataaagaa atttttgaag aacgtcttcg
    15901 tttgaatcta gatgacaaaa accgtcaatt tgtaaaaatg tttgattcag aaccacttga
    15961 ttttgacgat atttttgaag aatattatga atacggacaa caaatcaaac aatacgtcac
    16021 agatacttcc gttatcttga atgatgcttt ggatgcaggc aaacgcgtgt tgtttgaagg
    16081 cgcacaaggt gtaatgctgg atatcgacca gggaacttat ccttttgtta cttcttctaa
    16141 cccagtagcc ggtggggtaa caatcggaag cggtgtaggg ccttcaaaaa tcaataaagt
    16201 tgtcggcgtc tgcaaagcct atacgtcacg tgtcggtgat ggaccatttc caactgaatt
    16261 atttgatgaa acaggagaac aaatccgcga agtaggacga gaatacggta cgacaactgg
    16321 tcgtccgcgt cgtgtaggct ggtttgattc tgtagttatg cgccattcta aacgagtttc
    16381 aggtattacc aacctatctt taaactcgat cgatgtatta agtggattgg aaacagtaaa
    16441 aatttgtacg gcttatgaat tagatggcga actaatctac cactatccag caagcttgaa
    16501 agagttgaac cgctgcaaac caatctatga agaattgcca ggctggtcag aagacattac
    16561 aggatgcaaa acacttgcag aactgccaga aaacgcacgt aactatgtac gtcgtatctc
    16621 agagcttgtt ggtgtacgta tctcaacatt ttctgtcgga cctgatagaa cacaaacaaa
    16681 catcttagaa agcgtctggg cgcaaatcta aggataaaaa cgacgactga atcaggacgt
    16741 tcttaccaga gcgagtcgaa gtgtttatct atacgataat aataacttga agaggtagtg
    16801 aaagagacgt ttagccttat tgccgatagg ctgtctcttg aacactgttt attcagaata
    16861 agggactgtg acaaaaaccg tgtcacagtc cctttttccg aa
//
LOCUS       DAHHZJ010000046        16818 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.047, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000046 DAHHZJ010000000
VERSION     DAHHZJ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.047"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(<1..454)
                     /locus_tag="NBL02_001245"
     CDS             complement(<1..454)
                     /locus_tag="NBL02_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCD2571942.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKE"
     gene            complement(559..1422)
                     /locus_tag="NBL02_001246"
     CDS             complement(559..1422)
                     /locus_tag="NBL02_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCD2571943.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1618..3717)
                     /locus_tag="NBL02_001247"
     CDS             complement(1618..3717)
                     /locus_tag="NBL02_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HCD2571944.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3937..5046)
                     /gene="rpoD"
                     /locus_tag="NBL02_001248"
     CDS             complement(3937..5046)
                     /gene="rpoD"
                     /locus_tag="NBL02_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCD2571945.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(5070..6911)
                     /locus_tag="NBL02_001249"
     CDS             complement(5070..6911)
                     /locus_tag="NBL02_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCD2571946.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(7093..9408)
                     /locus_tag="NBL02_001250"
     CDS             complement(7093..9408)
                     /locus_tag="NBL02_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCD2571947.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(9593..10453)
                     /locus_tag="NBL02_001251"
     CDS             complement(9593..10453)
                     /locus_tag="NBL02_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HCD2571948.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLCGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(10512..11033)
                     /locus_tag="NBL02_001252"
     CDS             complement(10512..11033)
                     /locus_tag="NBL02_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HCD2571949.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            11311..12072
                     /locus_tag="NBL02_001253"
     CDS             11311..12072
                     /locus_tag="NBL02_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD2571950.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            12098..14689
                     /gene="mprF"
                     /locus_tag="NBL02_001254"
     CDS             12098..14689
                     /gene="mprF"
                     /locus_tag="NBL02_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HCD2571951.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(14877..16064)
                     /gene="tuf"
                     /locus_tag="NBL02_001255"
     CDS             complement(14877..16064)
                     /gene="tuf"
                     /locus_tag="NBL02_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCD2571952.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            16432..16788
                     /locus_tag="NBL02_001256"
     CDS             16432..16788
                     /locus_tag="NBL02_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCD2571953.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         4617 a         3491 c         2946 g         5764 t
ORIGIN      
        1 tttctttttc atagcattcc tgacagattc catggaacgt tagccgatga tcttttacta
       61 aaaaatgata acgttcttct actacttgct ccacttcaag taaaagatcc tcttctactt
      121 cctcgatcgt gccgcagttc aagcatagta aatgatgatg gaaatgtttg gcgccttctt
      181 ttctcaaatc ataacgggct aatccatcat taaaacttac tttatcgaca attttcaatt
      241 ctgtcaagat ttcaagggtt cgataaacag tagcaagtcc aatttccgga ctcttttttt
      301 tcactaaaaa gaagatttct tctgcagata aatgctcttt ctcattttcc aaaagcacaa
      361 gcagagtagc ttctctttgc ggtgtcagct taaaccccgc ttcatgtagc tgctgttttg
      421 tttttttcat cgccgaaata gcattcatga ccatttactc aaccccaaca atttataata
      481 attacaaata aaaaaagata taacactatg ttaattaaaa ttattataag ctagttaaga
      541 aaaacatgca agggattctc aattagattt tttgactagt tttgtcacac cgttttcttg
      601 tgtaatcaat ccttgtttca aaagattacc taatgcgcgc ttaaatgctc ctttgctgat
      661 accaaaagct ttcatgattt cttctggatc cgatttatct gtaaagttga tttgctgatc
      721 tgcagaacgt tctaaaatgg ttaaaatcat ttgtgcatca tctgatatag cttcatatgc
      781 tcttggcttc aatgaaagat tcagtacacc gtctggtcgg acaccaatca ctcgaccttt
      841 tatctgctcg cctagacgag gttcattata tcgttcagaa ggatggataa acccaagata
      901 gaaatcttcc gttaaaacgt acgtacctgt taatttcaag cggtatgctg ttcctatcac
      961 atctttgttt ttcatttctt ctgttccata tttgcttaaa gatttgaata ttttttcatc
     1021 tgctaaactg ccccaaatac ggtcttttgc atcaactcta agggaaatca tcaagcgatc
     1081 ccctttttta ggccatagct ctgtcattgt cggcaattca tcaagagata cgacaaagtc
     1141 tttatctgga agaccgatat cgacaaacgc acctaaatct tttcgtgtag cagtaacgat
     1201 tccaaaagcg taatgaccga tacggctttt aggaatttgt gtagtgatcc gattttcatg
     1261 tttttgattt tggtaaccga atccttcaac tgcttcaccg attttgtgtt caccttctga
     1321 tttgcttaag ccaaaggttt gtccgttttt ttggacaaag tagttttttt cattttcatc
     1381 aataataata cctgtaaata tagttgctaa taaatcgttc atttttttct ccctctatag
     1441 tgaaaggttt tcttaataca tttctttgct atcataacat aatgttatct acttgaatag
     1501 ctgagccttg gtttggaaag gtgaaaaaag gagtatgaca acagttgtgt catactcccc
     1561 tttttcgaat aaacggtgtt aaacggcagg ctcacagcct ctttcatttg cctaaaatta
     1621 attgctccca tttcctccga accatccagt tttacagttg atggttacga tgtaaaatgg
     1681 caaagcactt tctccaatgc ctttcatcaa tgcagatttt gttgaagtgg catatagatt
     1741 ataatagcct tccccatttt cgattctggc tttttctaat ctgtaacctc cttcaacgat
     1801 atatccacgt tggatacatg tagcaatcac tttgctttgg acacctggtg cccacgtcca
     1861 tgctggatta gatgcatcgg caatatcact tgctctggca gatttgattg gctgattcgc
     1921 tgagtttgaa gaggaagggg tcgaggatgc cgcttcttgc tgtgctgctt gtgcgaccgc
     1981 agcttgttga gcggcttgag ctgcagctgc tgcttgagcg gcttgttttg cttgttcttc
     2041 agctgctttt ttcgcttctt ctgctttttt cttttcctct tcttcttttt tctctttatc
     2101 agtgagcatc ttatctactt gtttcaattg ttcattcaac tgagcagata gatctgtatt
     2161 tttcactttt tctactgctt cttttgccgt cttgtattgg tcgcgtgttg ctgaatccac
     2221 tacttttccg tctttaaaga ctatagctgt tttttcttta gctgtttgca attcagcata
     2281 ttgattttta gcttctgttt gtgcttgttg aatcaatttt ccaaaatctg tgttatccgt
     2341 ttcgattgct tggatctctt tatctgcttt caacttgatg tcatctgcta gatgatcatc
     2401 gacgatggca ggtgctatga accactcatt cactttttgg atatctcgga tcttagattg
     2461 gatatcttgg taagttgttt ctaactctga atatcctttt tcattcttaa gactggataa
     2521 atcatttttt agttttgata aatcttgatg aatcatatct gcacgaataa attgatggtg
     2581 atcatctgta taaaaggcag caagtttctc atgaagttct tccatttgtt gctgtctttt
     2641 tgcttctgct gctacttcgg cttctacttt ttgatgatgg tcataataag cccagcttcc
     2701 tccaccgagt agaagtactg ctgctaaaac gagataaggt actttttttg attttttctt
     2761 ttgcgcttta tctggatctg ttttttctaa gtttgattct tgatgtgttt tttcagtatc
     2821 tggcacgatt gttattgatt tcgcttctgc ttcaaacagc ttaggctggt tctcttcttt
     2881 ttctattttt gcttcaagat cagtaccctc tttgcttaca gattgatttt cagcatcgga
     2941 ttcactattt atttcatcgg gctctgttga ctttttgtct gtgttttcga gtatttcttt
     3001 tttctctgct tccggcagtt tattcgtttt ttccttttct aattgttttt cagtaaatac
     3061 ttcagttttt tctgtgatat ccgtttttac gtcttttatt tttactgttt ctttacctgt
     3121 ggcgttttct ctatcagagt ctttttcagt ttcttgttcc ttttctggct ctactttgtt
     3181 ttcctgtgca attttttcac tgatcatttt gagcagctct ttcgcgtgct cttcttttgt
     3241 atcttcacga tgtgctttga tataagcgga tagtatcgaa ttttcttcct cattatcgac
     3301 gatttctttc agttcagcta ctcttctttt ttgttttgat tcaagcgttt cttcttgatt
     3361 ctttcctgat tcttccgaag tctcatgaac agttgctttc ttctgttcat ttttctctat
     3421 attagttact tgatcgtgag caggtggttc ttcgtcttct ggttctgctt tttcgaataa
     3481 ttccattaca gattctagag gtaaatcttt caattccgac cattcgattg gatcgtttaa
     3541 ttctgtatca ggaaagactg catcttcagt tgatttatcc tgcatagctg catctgtatt
     3601 tgtttcttca attgattctt ttatttgctg atcttctgca gttatttgat gaccgcagtc
     3661 tggacagata gtattctgct ccattttttt cgatccgcaa tttggacatt ttttcatcac
     3721 ttagcgatcg gctcctttac taggtttacg ctaccattta atctatcata ttctatacgt
     3781 cacgccaata gcttattttt aggatttact taaaaataat gaatgaattt ccttcctata
     3841 gaaaaaaaag cttgagactg cgtagtctca agcttttttt cttttaaaat aaaccaaaca
     3901 gttttactga tttctcttct ggtcaatagt ccacgcttat tctaagaaat cttttaattg
     3961 ttttgaacga gaaggatggc gcaattttct taatgcttta gcctcgatct gacgaatacg
     4021 ttcccgagta acaccaaata ctttccctac ttcttccaat gttctcgtcc ggccatcatc
     4081 taaaccaaaa cgtaagcgaa ggacattctc ttcccgatca gtcaatgtat ctagtacatc
     4141 ttccaattgt tcttttagta actcataagc tgcgtgttct gctggacttg ttgcttcctg
     4201 gtcttctatg aaatcaccta agtgagagtc atcttcttca ccgattggcg tttcaaggga
     4261 aacaggttct tgcgcgattt tcaagatttc tcggactttt tctgttggaa gatccatttc
     4321 cgcaccgatt tcttctggcg taggttctct tccaaggtct tgcagcaatt gacgttggat
     4381 acgtatcaat ttattaattg tttcaaccat atgaactgga atacggatcg tacgtgcttg
     4441 gtcagcgatt gctcgagtga tggcttgacg aatccaccat gtagcatagg ttgaaaactt
     4501 aaatcctttg cggtagtcaa atttttcaac tgccttcatc agtcccatgt ttccttcttg
     4561 gatcaagtca aggaactgca tgcctcgtcc tacataacgt ttagcgatac ttactactaa
     4621 acgaaggttt gcttctgcca gacgctgttt cgcttcttgg tcgccttctt cgattttcaa
     4681 tgctaacgct acttcttctt ccgcagttaa aagggagaca cggccaattt ctttcagata
     4741 catacgaacc gggtcattga tttttacacc ggttggcgca gaaagatctt ctgtttttgc
     4801 ttgttcagct tttttctcag cactcttcaa gctgtgaatt gatggatcac cattttcatc
     4861 caccacactg atacctgcgt cttcaatctt ttggatcaat ttgtccattt catctgcatt
     4921 caaagtgaat ggtgtagcaa gctgattgga taaatcatca tatacaactt gtcgtttggg
     4981 tttgttttct ttgatgaaag ctgctactgc tgcttcatac gtttttttat ctgttccatt
     5041 tgccataaaa gaaggccccc cttgagtagc tatttggctt gttttaactg tttcgttaaa
     5101 ttgatgattt caatcgtcaa ttcaagctca agctgtttgt ttccagttcg tctggcttct
     5161 tgctgcattt gtttttttaa tgttatttca tcagctaagc cagatttgct gatcacatct
     5221 agcaagtctt ggatttctcg tggcgaactt tcttctgcca ccggtaaata ttcgatttcc
     5281 accaccaacc gccttaaatt ttcatcttgc aaaaagtcta aaaactctgc aaggttgaat
     5341 tcataatgtt gtgagacata tgcatcgaat aaaaaataaa cttctgcata tgcatcatga
     5401 ataaactgaa attctcgttc ttttaaaata ttacggacag aaacttcttt aaatgcacga
     5461 tataatagaa tctgctctgc cttttctact tgcgtcaaag gtctcttttt ccgtgcagtt
     5521 tcttgtctcg taacctgagt gtgttgactt tcctgaggtt cttgacgatt ttgctgattt
     5581 gtccttcgaa gatcgcgcag ttgtttttgt atcgtctctc tgccgatctg aaactctgtt
     5641 gacagttgag atagatacat atcttgttcg attggtgaaa caactcggct aagttcaact
     5701 agtaattctt cgaggtattc gatcttgtct ttttcattat ctaaattttt cccttgtttt
     5761 aaataacgtg atttaaaagt aaagactgtt tcgcgcccat gagccactaa ttcttggaaa
     5821 gatgcttctc catacttacg gagatattca tcaggatcaa gcttttctgg aacgactacg
     5881 acagaaagtg ctaatcgact gttttgccga agcaattcaa tccctcgatt ggttgcttca
     5941 aaacctgcgt tgtctccgtc atagcaaaag accaattctt tcgtcagctt ttccatttgt
     6001 tggatctgtt gagctgttag acttgtcccc atagaagcta atccagtttt gataccagcc
     6061 tgccatgcag caatcacatc cataaaccct tcgaacaaca aaatttctcc gtttttccgg
     6121 atatcgcttc ttgctttatc aagattaaat aaaacttgtc ttttattgaa cagttctgtt
     6181 tctggactat tcaagtattt gggctgatct ttttcctgcc ctttttcagg tgcaagccag
     6241 cgtcctgaaa aaccgatcgt ctggccttga gaattacgaa gtggaaacat cactctttga
     6301 taaaaacgat cagacatacg gccatcatca tgcgtgacaa acaatccaga gcgttctaac
     6361 aattcatttg aaattgattc attcttgaat acttgctgaa gaaaagaacg ctcattaggt
     6421 gcaaaaccta tgttgaattc ttcaattaat tctaaagaca acccacgact ttgcaaatat
     6481 tctaaagctg gctgtccagc ttttgtatgc aatagcatgt gatgatagat ttccgctgct
     6541 ttttcatgag cagcaatcag tttccccgta gcagattcag gattttggac agctatagta
     6601 cgctgacgcc attgatcttc taacggtatc tgttcgaagt ctgccacttt taccactgct
     6661 tctggaaaac ttagaccttc tagttcttga agaaaagtaa acacgtttcc gcctttacca
     6721 caaccaaagc aatgaaaaat ctgcttgtct tctgcaacag aaaaagacgg tgtacgttct
     6781 tcatgaaaag gacacagccc caagtaattt ttaccagact ttttcaactg gacatattgt
     6841 ccaatcacct caacgatatt cgtttgactg cgtactgttt cgataacctc ttgaggaatc
     6901 cgttgagcca ttttttcacc ccctgctcta ttgatatctt ttgggtttaa cagaagatat
     6961 ttcaggagaa taaccggtaa aaaatcgcac taactgaaat cagtgcgatt ttaaaggaca
     7021 caattataca tgtttgattg tatcatattt cattgctttt tcaagtataa cacatttcta
     7081 tctcattaag catcaatttg ctactctttt cagtaactga ttttgaatat cagccttcca
     7141 gtacaatact cgaagaacaa tatttacgag catgaataca aagaagtatc ctacgctctc
     7201 tcccaaccct aagtttactt ccaaaattcc atggaacagc attgcagcta catatatccc
     7261 tacgatcgtc gttactgcta ggaagatgcg caatgggttg aagggataac aagcttttac
     7321 aactgccatg caactgatcc caatcagcaa gtaatacatc aaagtagtag tttcagcatt
     7381 tccccatgcg aatgtttttc caagtagata caccgcaatg atattcacta ctacaagcaa
     7441 ggcatttggt aaagcattaa taagtgctgt aggcaaataa cggtaagtga tttttctttt
     7501 atcttgttca aaagataaaa agaaggaagg atacccttca atcgctaaat caatcagcgt
     7561 gatttggata gggataaacg gaaatccaat tgctgtaacg gcacatataa tcgataaaat
     7621 aaatgaataa atcgttttga taaaaaagat gcctgagact ttcgtcacat tgttgaccac
     7681 tcggcgtcct tcaaacagga cttctggcaa tgtagtaaaa tcagagtcca acaataccag
     7741 attcgcaatc tgacgtgttg ctccatcacc ttctgccata gcaatgctgc aatctgcttc
     7801 tcttaacgcc aaaacatcat tcacaccgtc gcctgtcatt gcaaccgttc tgccgctttc
     7861 tttcaattca tttaccagca atttcttttg ctgcggactc actcgtccaa aaaccgtata
     7921 gcgatgagca gcttctcgca cttccatttc ttcagtaatt tgagaaagat cgacatacgc
     7981 atcataattc ggtaatcctg cacgtctagc gatattggag acagtaaaag gattgtctcc
     8041 agaaatcact ttcagatcca caccttcctc atgaagataa gctagtgttt cttgagcgtt
     8101 ttttcggatt ggatcatcga tttcaaacag agcaaccggc tcaacaaatg ctggtttttc
     8161 ttcgttcaat tgctgatttg gagcgatccc caacataagt acgcgatagc cattcgcttg
     8221 ggctgttttt agactgtccg gaagccgatc ttcttccacc aaacgttcag gcgctcctac
     8281 gtacacgact cccagttctg gaaattccat cgcgccccat ttgcgatcag aagaaaatgc
     8341 gactttgttt gtcacatcat accgattaga aagctcaaaa taatctctca tagcccgcat
     8401 agtgatgttg ttatcactgc tttctgccag atacgtcccc ataatttctg ggatggcttt
     8461 ctcgtacagc ggatgtagtg cttctacccg ctggatactc attttccctt cagtaatggt
     8521 ccctgtttta tccaagcata atgtatcgac atgggccagc gtttcaattg aatacatgtc
     8581 ttgaacaagg attcgttttt ttgccagttt ggttacccct gtcgttaatg caatacttat
     8641 tagcaatacc aaaccctttg gaagcatgcc taacaaagct gctgcagatg caataacaga
     8701 aattttgata gaagcatcgc ggatgaacag tgcttccaaa aatagtatga tccccaaagg
     8761 aataatcacg aaactagtga atttggatac tttgcgaatc gaatctacaa gttcagaacg
     8821 aatagctttg tgagtctttg cctcgctagt caagcggaca gcataattat ctgcacctac
     8881 tcggataact tcagccatga cagaaccgct tgtcaaaaag cttccagaaa gcaattcagc
     8941 tccagtttct ttgacaatca aatcagattc gcctgtcaaa agagcttcat ttgcttctgc
     9001 ccgtccagat ataacctgta aatcggctgg tatctgttct cctgctccga tttttaccaa
     9061 atctcctaat accagctctt tagtagaaat cgattgttct ttaccgtccc ggatcactag
     9121 tacttctgat tgggaaacaa tcgataattt ccgtacaaga tttcttgcat gtatttcttg
     9181 ataaattcca ataacgatat tgacgacaat aattgctaaa tacgccatgt tggaatacgc
     9241 acctacaaat aacagacaaa tcccaatacc aaagttcaga aaattgaata acgtcaatat
     9301 attatcgctg aatatttgtt ttgtactttt tgatgcatct tcctggtagt cattttgctg
     9361 gccagaagcc attttctgaa ctacctcttg ctgtgtaagt ccattcattt tctttttctc
     9421 cattttccgc caactttcaa aaataatcac gtgtttaagt ctagcgtgtt ccaagattaa
     9481 aaacaacttg tttttataaa atttatagaa aaacacacga atgattttac aaaaaagatg
     9541 ccaacctttt tgtttgaatc acccgtgtgc tgtttttatt atcaatctat ctttacttat
     9601 attgttcctc caaacggctc atccaccaac ctagtgctgt accggctata aaaagaacag
     9661 gacaagctag taccccagca actcccaatc ccgcgtaatc ggtaggaatg atcataatcg
     9721 ttgaagcaaa aactacacct aaaatgaaat ggaatagctt gctatatgcc tttgagaaaa
     9781 tgtaatccat gatttttgac aatccaagaa cacaaagaat cccaccaatt ccgatcggca
     9841 caatcacttc aagtcgtact tctttaaatc cgtcagccat cgctttatac aatcccatat
     9901 acaccaaaaa attagaaggg ctcaatcctg ggacgatcat acccaaacca atcaatccgc
     9961 cggcaatcag ccatgttcca aaattctgat cgacttgccc gtcaaataat tgagcgccgc
    10021 ataataaaaa caaataagcc agaacaaaag taacaaccat gatgatcaca tcagaagtat
    10081 ctcttccttt tttccctgct tctttccata acgcaggaac agttccagcg atacatccga
    10141 caaaaaacca taatatcgtt gtttcatacg cacctaataa aaaactgact aaaaaagaaa
    10201 gtagaaagat gcctgtcact ccgcctagac caacaggcag aaaaaacaaa acattttctt
    10261 tgaaattttt tgtaaggtgg gctaaaaaag aaatcagccg ctcatacatg ccaaagacag
    10321 ctgctaatgc accaccgctg actcctggca agataaatcc tgaaccgata aacattccct
    10381 tcacaaaccg taacatccag tctttggttg tgtaccccac ttcttgattt tccatttttt
    10441 gccttttttc cactatttgc gcactccttc tttcatttaa ttttattcat gattgaacat
    10501 ttcctgctat tttacttaaa gactttttca taggtctcta ttattttttc attcattttg
    10561 acaatatcag gataaaacat gttacctcca ttatgataga gatagccgcc attgtaaagc
    10621 aaggactgga tccgccaata tcggtattgt ttcccttcct catttcctaa aagtgggctc
    10681 agcacatctt tagaatacgc ttctgctaat tctactgtgt tttctccgcc atgatttttt
    10741 acataggcaa tataatcttt cccaaaatta tatgcttgga cagctgtcca taaatcgcat
    10801 ccttgtttct ttgcttctac tatcatttcc gccaaatatt tgacaccttg atcaatactg
    10861 tcagctggat tatcaatgat ctcagcttct ccatgcagac tttccgagct ctgcatgaga
    10921 tctgtccctt ttccttttga ttcggtcaat attatcgcct tgatcaaatt ctcgtagttt
    10981 tccatatgat aattttttgc agctgttgtt atttctattt gatagctttc cactcgtttt
    11041 accgaacgat aagttcttcc tcataaaaac aacaccgcac caataaccgt cagaatgatc
    11101 atgtaaaaaa gaatacggat aatagataat cgttttcgct tcatttcatc acctagttat
    11161 tttgacatct ttttttatct tactatttct ttatcttctt ggctatttct tccttgattt
    11221 ctgtgtacgc cttaagaaaa aatgaatttt cctgtatttt ttgacatctt acgaaaatga
    11281 tcgttgtttt tcagtaaaaa caattgccca atgactgata taatcgttat actttacaca
    11341 gtaggtgatt atgtgaaaaa agaaaagaaa tatgcaaaaa tgccagatgg gagcgaaatt
    11401 tactatgaaa agagcggtca aggcttccct ctctttttac tgcatggcaa tgacggcagc
    11461 ggccgctttt tttcagaaca ggtccctgta ctcgaacgct actataccgt gtatctagta
    11521 gatagccgag gacatggtcg ttcgacaaac gaagcatcta tgttgaattt tcagttaatg
    11581 gccgaggatc tgaatacgat catgttgctg gaaaaaatcg atcaggcaga ttttcttgga
    11641 tttagtgatg gagcaaatct agcactggta tttgccagca gcttccctaa aaaagttcat
    11701 cgattgattt taaattctgg aaacacgctt gttaaaggcg ttcgtttttc tgccagagtc
    11761 atcagtaata ttcattatgc atgggtatgg ttactctctt tatttcgccc aagtcttcgc
    11821 aaaaatcttt tagttatcaa acttttgctg catgacatcg ggcttacaga aaatgattta
    11881 aaaaaaatca actcccccac tttgatcatc gtgggaaaaa aagatgtaat caagctaaaa
    11941 cattctttgt atatagctaa aactatacca aaagcttcct ttgtgcttgt gaaagaacaa
    12001 ggacatgaat tagctagaaa agatcctgaa cgcttcaatc gagaagttct tcaatttctt
    12061 tctgaaacat aactattagg aatagaaggt gatttatttg ttaaaaaata ccatacaatg
    12121 gctgaaaaac cacctaggac tgttcaaaac aatctttttg atttctgtca ttgtcattat
    12181 tgtcggtgaa ttgatgtcca tcggtaaaac tctatctatc cagcaattag gagaaacgtt
    12241 tgccactata cctgtatgga aaagcggact aatgctattg attggtctta tctctgtgtt
    12301 acctatggtt ggttatgata ttattttgaa tcggatgctt gaacaaaagc agaatcctcg
    12361 ttacctgttt gagacaagct ggctgatcaa tacgatcaac aatattgccg gtttcggcgg
    12421 gtttgttagt gtggggcttc gctctgaact atatggacaa aagaaagaga gcagaaacgt
    12481 gatccaagca ttatcgaaag ttttcttgtt tttgatggct ggactttcta tttacagtct
    12541 gctttctttt ttacttgtag tcttcacgcc agttgctcct tttttgaagc aatattggat
    12601 atggttgatc ggcggcggtc tatattttcc tgtcgtcttt ttatttacta ctttcaaaaa
    12661 gaaaggattc cttggtggta cctcacttaa aactcaagga cagctattgc tagtttcatt
    12721 gttggaatgg accggtgtcc tgtttagttt cctttctgtt ggttatttaa tggaaatccg
    12781 gattgatctg tggcaaacaa ttccattatt cattgctgct tcagtgatag gaatcgtgtc
    12841 tatgattcca ggagaaattg ggagtttcga tgtaatgatg attatcgggt tgtccgctat
    12901 tggtgtgcca agagaaatag tcgttgtgtg gatcttgctc tatcgcctgt tctactacat
    12961 cattccgttt ttgattggaa tcgtcttttt cttcaaaaat atcggttcca cttttgatca
    13021 gcgttattct ggtatcccta aacaattagc aacagaaatt gcgcataaaa tcgtggttgt
    13081 cttattatac ttttctggaa tcatgctagt actttctgcc actatcccgc aagcttttac
    13141 agagtttcgc tggcttcata gtttgaaccc attgaaattt cattttatta tccaatttcc
    13201 tagtatacta ttgggatttc ttttaatcat tatgggcaga gggatcgcag cacgtgtgaa
    13261 acgagcatat ttgcctacaa tcttcttgat tgttctagct ttactttatg ttttattaag
    13321 tgattttagt ttcacaccgg tggttttctt gtctattctg cttctgatca ttcttgcttc
    13381 taaaaacgaa ttatttcggg aacaattgat ctattcatgg gagtggcgta cgatcgatgg
    13441 aatcattatt ggtgcactgt ccttgcttta catcgttatt ggggtataca atctgccgga
    13501 tttccctcat cggcgtcacc attttatttc ctttttctta tttccttctg aaaagatttg
    13561 gttttcggga ttgttggcca tcatcgctgt tagtttcatg atcgttctat ttgttcattt
    13621 ccttcaagga gaaaagaaac agattggcga agcctttaat gaagaaaagg ccctgaagat
    13681 ccttacaact tatggaggaa actccgatag tcaattgatc ttcttaaaag ataagcggat
    13741 gtttgcctat gaaaaagacg gtgaaccaac tgttcttctc caattcgctt gcttcaataa
    13801 taaatgtatt gtaatgggtg atccttctgg taagaaagaa gattttccag aagcaatcga
    13861 agcttttatc gaagaaacag atcgactatg ctatttacca gttttctacg aaacaagcga
    13921 agagattgtt atgattttgc atgaattcgg ctacgatttc attaagatgg gggaagaagc
    13981 ttatgtagac ctgaatagtt ttacaacttc agggaaaaaa atgaagggca ctcgtgctgt
    14041 cttgaatcgg atcgaacgag aaggatttac atttgatgtc ttacaaccac cattttctac
    14101 tgagcaaatg agtactttca aaaatatctc tgataattgg ttgggttcac gaaaggaaaa
    14161 agggttttct ttaggctttt tctctgaaga ttatctacaa agagcgccta ttgcagttgt
    14221 caaaaatacc caagaagaaa ttgtcgcttt tgcaacgatc atgcctactt acacgaacaa
    14281 taaagtagga acgattgatc tgatgcgata tgattctaaa aaagcacctt ctggcagtat
    14341 ggatttcttg ttcttgcatt tgtttgccta tatgaaagaa gaacatatcc aatggttcaa
    14401 tttagggatg gcccctcttt caaatgtcgg aacttccaga aagagttttt tacaggaaag
    14461 gattgcatac ttggtttacg agtttggttc tcatttttat tctttccatg gattaagaga
    14521 atataaaagc aagtacgcta caaactgggt ctcacgttat actctatatt ctagagatag
    14581 ttggattgca tatgttatga ttgctttgct gattattgat aatgcgccag ttgagcagac
    14641 acaaggtaga ataagtggtt tgaaaaaatg gatacgaaga aagtattaaa aactgcatac
    14701 cgatagatga acactctcat gatgaataaa agagactgaa gtaaaacgaa agttcatgct
    14761 tctacaatct cttcttatac aattgaagaa caaagagcgg tccaaacaga aatcctgttt
    14821 ggaccgctct tttattttaa acatttttca ttaccaaaat attcgagtcg cgtttcttat
    14881 gcttcgatct ctgtaacgat acctgaacct actgtccgtc cgccttcacg aattgagaat
    14941 gtcgttccgt tttcaacagc gattggatgg atcaagtcaa catcaatcgt cacgttgtca
    15001 ccaggcatga ccatttctgt atcttctggc aatgtgattg ttcctgtaac atcagttgtg
    15061 cggaagtaga actgtggacg atagttgttg aagaatggag tatgacgtcc accttcttct
    15121 tttgtcaaca cgtacacttc tgctttgaat tttgtatgtg gagtaatgga acctggttta
    15181 gcaagtactt gtccacgttc gatatcgtca cgttggatcc cccgtaacaa cacgcctacg
    15241 ttatccccag cttccccgta atctaacgtt ttacggaaca tttctacacc tgttacaact
    15301 gctttttgtg tttcaggttt gatccctacg atctctacct catcaccgac acgaacagca
    15361 ccacgatcaa tacgacctga ggcaacagtt cctcgacctg taattgagaa gacgtcttcc
    15421 actggcaata gtaatggttt atctgtatca cgttctggtg ttgggatata ttcatctact
    15481 gtatccatca attccataat agcagcttca gcatctggat cgccttgcaa tgcttttaat
    15541 gcggaacctt tgatcacagg agtgtcatcg cctgggaaac catattcgct caataactcg
    15601 cgaacttcca tttctaccaa atcgatcaat tcttcatcat cgactaaatc aactttgttc
    15661 aagaaaacaa tcaggtattt tacaccaact tggcgtgaca ataaaatgtg ttcacgtgtt
    15721 tgaggcatcg gaccgtcagt tgcagaaaca actaagattg caccatccat ttgtgctgcc
    15781 cctgtaatca tgtttttcac atagtccgcg tgtcctggtg catcaatatg cgcgtagtgg
    15841 cgtttttctg tttcatattc tacgtgtgcg gtattgatcg taatcccacg ttcacgttct
    15901 tctggagctg catcaatact agcatagtct tgaggatttg ctaggccttt tttgcctaat
    15961 actgtagtga tcgcagcagt taaagtagtt ttcccatggt caacgtgtcc aatcgtacca
    16021 atgttaacgt gtggtttact tctgtcataa tgttcttttg ccataattta caaacctcct
    16081 aaataatata gatcactgaa aaatttcagc tctcaccttt ttattatagt ctttttttga
    16141 ctaaatcaaa aagaatacgt ccgacttact ttttgtaaga ttcgaactaa gcttattata
    16201 cgtcaatatt ggtcaaagtc aactaagagc actcgtctgt cttatacgcg aaaaaagtac
    16261 cttttataaa taaaaaaatg tgttttttga gaaaaacttc aagatagtcc ttaactttct
    16321 tttgaagcaa taagaacaaa atgaggaaaa gtttaaaatt cagctgttgc caacaacttt
    16381 caatagacga aatcttctat ttcgttaata ataaggaaag aggtgaagta catgaaaaaa
    16441 ttaattggcg tacttgtcgt tttaggcata tttctcggcg gaagttttgc agcttatcat
    16501 tatttttacg gcggcgaagc ttattataca aaaatcacga cgaatggcga aatgtcagct
    16561 acccaagcag ataacggaga gaaattcacg acttatacct acaagcaagc ggcttataat
    16621 aaagatggtg aaaaaaaaca ggtgacgctt cgcgaagaaa gagaacatcc attaaagatg
    16681 aatgcgtatc ttaaactcaa agtcaatcct cgtaaaggtg tattaagctg ggaagaaata
    16741 aaggcttctg aagtgcctaa aaatgcggca gaaaaaatcg ataattagta aatggtctta
    16801 aaattttatt agataaaa
//
LOCUS       DAHHZJ010000047        16567 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.048, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000047 DAHHZJ010000000
VERSION     DAHHZJ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.048"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..598
                     /gene="scm"
                     /locus_tag="NBL02_001257"
     CDS             <1..598
                     /gene="scm"
                     /locus_tag="NBL02_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HCD2571954.1"
                     /translation="SDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTS
                     STTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSK
                     RALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(753..1739)
                     /locus_tag="NBL02_001258"
     CDS             complement(753..1739)
                     /locus_tag="NBL02_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCD2571955.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1762..2328)
                     /locus_tag="NBL02_001259"
     CDS             complement(1762..2328)
                     /locus_tag="NBL02_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HCD2571956.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2342..2758)
                     /locus_tag="NBL02_001260"
     CDS             complement(2342..2758)
                     /locus_tag="NBL02_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HCD2571957.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(3215..5149)
                     /locus_tag="NBL02_001261"
     CDS             complement(3215..5149)
                     /locus_tag="NBL02_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCD2571958.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5430..6386
                     /locus_tag="NBL02_001262"
     CDS             5430..6386
                     /locus_tag="NBL02_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HCD2571959.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6476..7579
                     /locus_tag="NBL02_001263"
     CDS             6476..7579
                     /locus_tag="NBL02_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HCD2571960.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7592..8545
                     /gene="menA"
                     /locus_tag="NBL02_001264"
     CDS             7592..8545
                     /gene="menA"
                     /locus_tag="NBL02_001264"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCD2571961.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9359..10705)
                     /gene="gor"
                     /locus_tag="NBL02_001265"
     CDS             complement(9359..10705)
                     /gene="gor"
                     /locus_tag="NBL02_001265"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCD2571962.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFCGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10839..12092)
                     /locus_tag="NBL02_001266"
     CDS             complement(10839..12092)
                     /locus_tag="NBL02_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCD2571963.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(12098..13021)
                     /locus_tag="NBL02_001267"
     CDS             complement(12098..13021)
                     /locus_tag="NBL02_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCD2571964.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(13242..15734)
                     /locus_tag="NBL02_001268"
     CDS             complement(13242..15734)
                     /locus_tag="NBL02_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HCD2571965.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15741..16208)
                     /locus_tag="NBL02_001269"
     CDS             complement(15741..16208)
                     /locus_tag="NBL02_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HCD2571966.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(16344..16417)
                     /locus_tag="NBL02_001270"
     tRNA            complement(16344..16417)
                     /locus_tag="NBL02_001270"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16381..16383),aa:Arg,seq:acg)
     gene            complement(16475..16548)
                     /locus_tag="NBL02_001271"
     tRNA            complement(16475..16548)
                     /locus_tag="NBL02_001271"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16512..16514),aa:Pro,seq:tgg)
BASE COUNT         4894 a         3481 c         2948 g         5244 t
ORIGIN      
        1 aagtgacagc acggacacgt cagcttcaag cgacagtaca gatactacct ctaccacaag
       61 tgatagcacg gacacatcag cttcaagtga cagtacagat actacctcta ccacaagtga
      121 tagcacggac acgtcagctt caagcgacag tacggataca tctaccacaa caagtgatag
      181 cacggacacg tcagcttcaa gtgacagtac agatactacc tctaccacaa gtgatagctc
      241 agattcatct accactacaa gtgatagcac ggacacgtca gcttcaagtg acagtacaga
      301 tacatcctct accacaagtg atagctcaga ttcatcaact tcaagcgata gtacagacac
      361 gtcgtctacc acaagcgata cacgtcatgc aagtgtctcc ttctcaaaaa caagttcgga
      421 taacaggacg aactttaact taactaataa aacaagcaat gacaatggaa acaaatcaaa
      481 aagagcactt cctaaaactg gaagtcaatc gaataattgg ataacactcg caggcgttat
      541 attacttgta atcggtttaa gaatcacttt tatgagttat aagaaatcga aaaggtaaaa
      601 aaatattcaa gataaaaaag agcaacgtcg ctaacggacg ttgctcttta tttttgcttt
      661 ttatcataaa tacaagaact acaattcaat ttgtcaaaaa acacgtagat ttccttctga
      721 gaaatttatt tacgtatcga acctttccct attcagtctt tgcgatccaa aatcagttgg
      781 gtaagggaaa agagattctc ttttgttcga agagaggtgg caggtaattt ctttatttcc
      841 ttcaaagcct tttctgtgta agaggccgct aacttcctag cttcttccac gcctcctgta
      901 tgttcaacga ttttgcgtac ggcgtcgaca tcttcttctg tcattgccgc tttcttctct
      961 aaataaggta gtagctttgg tttgtttttt tgcaaagagt agatcaacgg taaagaatag
     1021 accccttgac gcatgtcttc tagtacaggt ttgccgattt cctggctctc ttttgtataa
     1081 tctaaaatat catcgataat ctgaaaggcc atcccgatag aaagtccgat atctcctgct
     1141 ttttttgcga agcgctcgct tgttccactt tcaaaagcgc caacagaaca acttaattgg
     1201 aacagttcag ctgtcttacc tgaaatattt tccaaatatt cttttacaga aagttcataa
     1261 ttatagcgat catccatctg ccctaattcg ccattcaata ctttttccat actgcgagaa
     1321 ttcaattgga gacttttcaa ggaagtggag taatcagaca atagtttaaa acagcagaca
     1381 aataaatagt ctcctgcata gacagaaacg acattaccaa actgactacg gatattaggc
     1441 tgaccacgtc gcagatccgc ttcatcaacg atatcatcat ggatcagtgt ggccgtatgg
     1501 agcatttcga tagaagctgc caaagcgatg gctttgtctc gatcttgctc tggtccaaat
     1561 tgtgagaaca atagctgata ggcaggacgc aacagctttc cgccggcatg gatcgtttct
     1621 ttgattgctt gttcgacagg tttgtttttc aggcggacag tttcttccat ccgttttagt
     1681 gtttctgtca attctttact taaagcagga tagttttccc acattggatg tagcttcata
     1741 aaaaaactcc ttgtgtgacg attatttttt ggtaaacatt gggctccatt tttgtttggt
     1801 tccctgctca taatcttttt ggaatcgttg aagtgtctgc acatgtcgta aaagataatt
     1861 cgctgcgatc ccgattgcaa tccctgataa aatccctaaa actgaaagga ctggcaagta
     1921 caacatgact gtccatgatt gtgcaatgaa agaagccatc aaaagctgtc cgacattatg
     1981 catgaaaccg ccggccgcac taattccgat gatactgaca cgttttggtc ctaattgctt
     2041 gaccaataac attccaaaat aactaagcaa agcaccactc atactataaa ggaatgtgga
     2101 aatcgttccg cctagcaaag tagtcaggaa tagtcgtagc caaagtagta agaagctgtc
     2161 tcgttttttc atggtaaaaa tggcaacgat cgtgattaga ttggctaagc ctagttttgc
     2221 accaggcgca aaagaaaaag gataagggat catattttcc aataaaccaa tgatcactcc
     2281 ttgtgcaaca agtagcgaaa tataaatcat tttttgtaat ttactcatgg tgtgtgaagg
     2341 tctaataaat cagactgtct tcgtcacctc catctgttga acggacttcg atcactaatt
     2401 tatgtggcaa acagacgatg gtttcgccat ttttcgttgc ccagcctcga cgtacgcaga
     2461 tctggtcatc gcaatctgct tcttttatcc gtactcgatc gccatctatc tcgatcaagt
     2521 tataatctcc gtgttcatct ttgtaagtat atgtgttgct ctcttctcca gccatcaaag
     2581 ggaatgtttt gatttctgtg ccatccacac gcagtaccgc ttctttttct gctgttgcgg
     2641 tgttttgctg aagaccaaaa atcatgactg gtaaaaaaga aagaacagtc aaaagcaaaa
     2701 taataaaaac atcccaaggt ttgagcagac ttttttcagc aaattcttta aggctcattt
     2761 ttgttctcct ttttccagat taactcaccc aattttagca taaaaacaag aggttgtgac
     2821 acctgtgtct tgctttgagt aataagaacg aagaatagaa aacagctttc catgttttct
     2881 aattctttgg gcttattacc gagaagcagc aggtggaaca ccgtttataa caagaggttg
     2941 tgacagaagt gggcaacttc aagaaataag aaggaattta cgaaaattac ttttcaagtt
     3001 tttgtgaaat ttcggcttat ttttcgaaga agctacttct ggaacaccgt ttataacaag
     3061 aggttgtaac agaggttgtg acacctgtgt cttgctcatg gaggcttatc tcgggcatct
     3121 tattccttta attttacata aaaaggaggc tgtgacggtt tgtctcagcc cctttctatt
     3181 gaaatcttgc attcaaataa cgttctttat ctatttattt cttgccgtac tgggaacttg
     3241 gtctaccgta ccaccaatta cctagtgtac gttgtagcca tggaattacc cagcgatcca
     3301 acccaatcgc acgtccggaa ccgttcatca atgcgaaagc aacaaaaatg aaccaaatgt
     3361 ttacccagta aaacatgcct gataagcaga acgtgatcac caatacaatc gttgtcgcac
     3421 tgcatagcca tgtgaataat ccagcaatca atgctaatgc caaggcaatt tcaaagaata
     3481 ccatgaattt ttggaagaac attgctacat cattgtttgg catgatcact tgcatgatct
     3541 ttgtgaacca gtctggtgac ttctcaatca cagccattgg ttcttcaccg tatgcataac
     3601 tcaaaccaaa atgtgcagct tgagtcgctg cttctccggc acttgcccct gctccgctgg
     3661 cagctgtggt tgcatcacca gcagcagaag caccactggc aacatctgtt acattgtatt
     3721 cccatgtcca agggaacaca ctgtttccag tgaaccatga tccttcacca aaccaagtac
     3781 ttggtttgaa cacttcacca ttcccaatga tttttttgga agcttcaacg aaccaaacta
     3841 aaccatagaa aatacgaagt ggcacactcc ataagacatt gccgtaacga gaagaatgtc
     3901 ctcgagtcac gttgcgatca tctttgatat ggaaaaactc atgcattaaa tattggaaca
     3961 tataataacc agaacgaata tcaaagaagt atttcaaatt cacgatatgt ttcattacga
     4021 tagctaaaaa tccgcttaaa tggatcttat caaataggtt agctaccccc catttggcac
     4081 caatagaaac cataaatcct tgatagtttc ctttaaattt atgcttttct ccgcctttga
     4141 tcgaagccac gatattcgct gcggctgtgt gtcctgtttg ttcagcagct tgaacgatct
     4201 gcggtgtcgg tgttcctggg aattcttcat aaaagactaa atcaccaatg atatagatgt
     4261 ttttatcttc gtagcctttt gcctgcatat attcattggc gatcaaccgc tgtccgcgtg
     4321 ctgattctaa tccaaagtca gcagcgtcag aagttccttt gacaccagct gtccaaatca
     4381 gtgtgtgagt cggaatgttg cgtccgtctt tcaatttgat atgatcagca gccacttcta
     4441 cgattggtgc attcaataat aattccacgt tgtgcttacg caaatagcgt tcagcttttg
     4501 cagcatcatt tcgatccaac atattcaaga tcgtcggcat tgcctctacg accatcagtg
     4561 tgatttcgtt tggatctaat ttataatctt tggctaggcg atttttccag tcgatcagtt
     4621 ccccaatcat ttcaataccg gtaaatccag aaccacagac aacgaaagtc agcatggctt
     4681 tacgcagttc atcatctggt tccaatgcag cttttgctac tgtcttctca atatgttccc
     4741 gaatccttac agaatcttca aatgaccaaa gggtgaatcc gtgtgctttg acaccaggtg
     4801 taccaaagtc attcggctct ccgcccattc caaggatcag ctgatcaaaa ggataggaac
     4861 ctaatttggt cttgacgatt tttcgttctt tatcgatacc gattacagtg tctgtgacaa
     4921 gttgtacatt cttttgacga gcaaacaaat gctgcagatc gtattggatt gcttctggtt
     4981 ctacacgtcc gccagctact tcatgcaact cggtcatcat agtatggtac gaatgacgat
     5041 cgatcagagt aattgctaca tcttcttttt tcagtttctt tgccaagtat tttgtagctg
     5101 aaacaccggc atatcctgct cctacaacga caatattttg ctttgccatt gatcatccgc
     5161 tccttataga ctatctatat aatgaatata tgataattct tttagtttga tacttattac
     5221 acaatctcca tatattatat agatgttctg ttcttttgtc taactccttt tggatttttt
     5281 tcaaatttta aaataaagcg ttttttcacg aaatttgctg aaagaaaacg tttgaaatat
     5341 tacatgggtt atttatcaaa aatagttgac ttataggcta aaaagattag aatgtataat
     5401 gaatcacaaa gaaaaattgt ggactagaaa tggaatggtg gggtattaaa atgaagaaaa
     5461 aacgttttgt aactggattt gccgtattag cattttcagc tctagtgtta ggcgcttgcg
     5521 gcgcagatga caacggaagc tcaaactctt cttctgaatc atctacagca caatcttcga
     5581 ctgcaaaaag cacaacagaa tcatctgcaa aagttgtagc tggcggagat ttgcaagacg
     5641 gcacatataa attagaagaa aaaaattatt caaatggtta ccgtgcagtc tttgaaatgg
     5701 ttgtaaaaga cggcaaaatc actgaatcaa aatatgataa cgtcaatgaa aacggcgaat
     5761 caaaaacaaa agatgcagaa tacaacaaaa acatggaagc aaaatctggc acaaacccag
     5821 aaaaatacat tcctgagttg aatgaccaat tcttaaaagc acaaagcgca agcggtgtgg
     5881 aagtcgtaac tggagctact cactcatctg aaagcttcca aaactacgca caacaattga
     5941 tccaagctgc tcaagctggt aacacagata caatcgaaat cgacaatggt gctgatttga
     6001 aagacggcac ttacaagctt gaagaaaaga actactctaa cggctaccgt gtgcaattcg
     6061 aaatgacagt tgctggcggc aaagtaactg aatcaaactt tgattatatc gacaaagacg
     6121 gtaaatcaaa acaagatgat acagaatata atgaaaatat gaaagcaaaa agcggtacag
     6181 aaccaaaaac ttacatccct actctgaacg acgaattcgt caaagcaatg ggcgaagaag
     6241 atggctcacc tgctaatgta gaagttgtta caggtgctac acacagctca cattcattca
     6301 tcatgtacgc acaacaatta gtgaatgcag ctgaaaaagg cgacactcaa acaatcgaag
     6361 tagataatat cgtaacagaa aaataatcac ataatcatac taataaaagt tggagggtgg
     6421 gacgatagcc agattaggca gtcgtctcac ctttcaaaat gaagagagga aaaagatgcg
     6481 gaaaaaacta ctattttcct taatcacact gggcatctct tttatggtgc taactggctg
     6541 cagcaactca gcaaataaaa caaccactga aagttcagca gctaccaaga tattgaaaga
     6601 tccatattct gatgaacagt tcttgttagg gacttacgtt cgtatccgtg tgtatgatga
     6661 aggcaaagaa tcagcattga aacctgcctt cgatcgagta aaagaacttg gtgataaaat
     6721 cacgatcaat caaaaaggtt ctgagatcga tgaagtgaat gagcaagctg ggatcaaacc
     6781 agtaaaagtt tctgatgacg tctacacgtt agtcaagcgg gcctacgaat atagccaaga
     6841 ttctcaaggc ggattcgata tggcgattgg tgcaatcaca caattatggc gaatcggttt
     6901 tgatgacgcc agaaaacctt cccaagaaga aatcgatcag gcattgaaat tagtggatta
     6961 tcataagatc gaactaaatg acaaagaaaa aactgtttat ctaaaagaaa aaggaatgat
     7021 catcgattta ggtgcaatcg ccaaaggtta cattacggac gaagtagtga aagtcttgaa
     7081 aaaacaagga gtgaccactg ccatcgttga cttgggcggt aatgtctatg tcttaggcca
     7141 cagcccaaga ggcgaaaacc aagactggac agtcggcatc caagatccga acatggctcg
     7201 cggatctgtt ttaggttcta ttaaagaacg gaacaaaaca ttagttacct ctggaattta
     7261 cgagcgttat ttagaagtag atggcaaaaa ataccatcat ttgtttgatc caaaaacggg
     7321 ttatccgttc gacaatgata tcgcaagtgt cacgatcata acagataaat caattgatgg
     7381 tgacgggttg tctacagcag tcttttcaat gggtgtgaaa aaaggactgg aatacgtcga
     7441 aagcgaattg aataacggga cggaagcgat cttcgtgaca aaagacgata aagtatatgt
     7501 gaccgatcca atcaaagaca ccttcaaatt atcggaggac tcccattaca cgatgggtga
     7561 ccgttctgaa ctaaaataag gagtacgaac catgacacta aaaatctttt tagaagtagt
     7621 tgaaatacga acaaaggtgg ctagtgtatt tccttttgcg gtaggggtat tattctctat
     7681 cgcttttttt catgagatac attggctaaa cactttttta ttttttattg gaatgctagt
     7741 ttttgattta gcgactaccg ctatcaataa ttacatggat tatgaaaagg ctcattctga
     7801 agtatataag tatgaagaaa atgtcattgg ccgcagaaat ctatctcctg tattgattcg
     7861 aaacatgatt ttagctatga ttgcatttgt ttttgttatc ggcgttattc taacgatcaa
     7921 tacgggttgg cttttacttt tgatgggatt tatctgttgt tttattggag tattctatac
     7981 attcggaccg attcctttat ctcgaatgcc tttaggggaa attttcagtg gggtgactat
     8041 gggccttgga atttttgcca tggtcgttta tgtcaataca ttcggttcag atgtctatga
     8101 tctgacagtc aacttttcat ctggacaatt tgctttggta ggaaatcttt gggctatagc
     8161 agcaatcgta ttagcttcct tacccttggt gtttacgatt gccaatatca tgttggctaa
     8221 taatttgcgg gatcttgacc gtgatattga aaatcatcgc tatacactag ttttttatat
     8281 cggacgtcct gcgggaacag tcttattcca gctattgatg tatgcttgct atttcgtgat
     8341 tttaatgggg ttgttgagcg gtatctatga atggccgatc ttggtcacct tcttgacctt
     8401 gccaattgtg aagaagcatt tggatcaatt caagcaaacg ttaccccagc ctatcagttt
     8461 cagctattcc atcaaaaata tggtcttatt caatgccagt tatgtattgg ggctattact
     8521 aacaatcatt atgcaagcca tttaacagca tgaagaggtt gtgaaaaaac tgccctgcat
     8581 caagtaataa gaacagccaa ataaaaatag ctccttatat ttttcgtttg gttgggctta
     8641 ttaccgcaga tgcaagcttt tgaacaccgt ttatcaggag gttgtgaaaa agtcgcttag
     8701 tttcgagtat taagaaaaat tttgtgaaaa tggctttcca catttttgca taaatttggc
     8761 ttaataccga gaaactggct tttgaacacc gtttatcaga ggttgtaaaa tcagtgtttt
     8821 gtcctgagta ttagagaaac aaatggaaaa atagttcctc atattttgcc gttttttgag
     8881 ctaataccga aggacagctg attgaacact atttgttcgg aaaaagtgac taactatgac
     8941 acaatttttt tcacagtccc ttttttcact tgatttgatt cgtaccagcg aacttgacga
     9001 gaactttctt ggtttggtaa ctatatggta ttcttttaac atcctcgttt gcttcttcta
     9061 atttatttat acaaagtcac acttttttaa acatctgttc ttcgatacat ttctatttct
     9121 tttaaacatc gataccaata ttttgaactt aagagtgcat accaaatgga ttcatttact
     9181 cttcgtcagt tacgaagata atcaaaatct ccaacttccc tacttatttc gttttgagaa
     9241 ttatcgaagc aatcttacat aaataactaa aaaacaaaaa accagaaaat cagtatactg
     9301 attttctggt tttacatggt ttgttcaata aaaagctgtt ggaactgtta atattaaatt
     9361 atcgcatcgt tacgaattct tccgctcctg ttggatggat ggctactgta ttgtcaaagt
     9421 cttctttagt tgcacccatc ttgatagcca cggcaaaacc ttgcagcatc tcatcgacac
     9481 caacaccaat agcatgtaag ccgacgattc tttcttcttt tccgacacag atcaatttca
     9541 tttcgcattt ttggcgataa tcatttaatg caaagtacat tggggtaaag cgtgaacgat
     9601 aaactttcaa ttcattttcg ccatattttt ccaatgcttc ttcttctgtc aaaccaattg
     9661 ttgcaattgg tggatgcgta aagacgactg tcggaacaag tttatagtca agataagaat
     9721 ctttctttcc attaaataaa cgttctgata aacgtctacc agctgcgatt gcaacaggag
     9781 tcagatctaa tttaccgatc acatcaccaa cagcaaaaat atgttcatcc gttgtatttt
     9841 ggtatttatc tactttgata aacccttttt tgtccaattc cacatttgta ttttccagac
     9901 ctaatttttc ggtattcggt acacgtccac cggcaaaaag tacacaatca ccagttaagg
     9961 tttcaccatt ttcaaatagc accgtatatt cgtcattgtt tttagtaatt tctttagcaa
    10021 tatgatgtgc gtaagttttt attccagctt cttggtacat ttggatcaaa ttgtccgaaa
    10081 gcattttatc aaacgttcta agtggtcgtt cttttcgata tgcccacatg acttcactac
    10141 ctaaaccaca aaatacacct gaaagttcag ccgcaatgta tccagcacct aaaacgattg
    10201 ctctcttagg taactctgtt aattcaaaaa atccatttga atcaattgcg tattctccac
    10261 caggaatggt cattttactt gcacggcctc cagtagcgat caagatttta ggagcacgat
    10321 aggtttcacc gtttacttcg atcgtttgac tatcgataaa agtagcgtaa ccttttatag
    10381 cctcgacatg attgctatct aatcctcttt gataagcacc gtgtagaaaa tcaatatatt
    10441 tttctcgtct ttctactaga ccggcgaaat caacattttt taaatctgct tggataccgt
    10501 aacttgaggc atctcgttca attgtttcca aaatcgtact tgcttgccac ataacttttt
    10561 taggtacaca accgacgttg acacatgttc cgcccaattc attcgcttca atcagcaaaa
    10621 cttttgcgcc atgcatccct gctctgtttg ctgaagcaat accgccagag ccgccaccaa
    10681 ttacgatgta atcatatatt tgcattttgt tcctccttat tgaagagatc aatcaaaacc
    10741 tttatttaac gatccttttt ctacagaaaa caagtgtaac acttttcttg aattttgtat
    10801 gcgaagatgc ttaaaacagg gatatatcag ctttttcctt atttacgttt tcggatcata
    10861 tctccaggtt tcaccggctg tgcgactggc atagttaaaa tcatcattgg atttggtgct
    10921 cggtcaatgg attcgccttc ttcattatgc ataacttcaa cggtttgact gaaatgactg
    10981 aatcctggac cataaaattc gatttcatct cctacagaaa acaagtttct ctgacgtatc
    11041 gtagcaatct gtgttgcctc atcataagat aatacttcac cgacaaattt atatactggg
    11101 attttacgac gttcaccgaa taattgttcg ttttcagttg gtgtattgta gtaaaaacct
    11161 gtagctaatt ctcgttgtgc tactttccat aactcgtcga tccattcttg cttgcagaca
    11221 tagttttcag gatcttccat ataactgtct acagccgctt tatagacatt tgacaccgta
    11281 gatacataat ggatcgattt cattcttcct tcgatcttga agctgtctac gccgttttca
    11341 atcaattctg gaatatgttc gatcattgac atatccactg cactcatcga gaattcttct
    11401 tccacttcgc ctgtatcagt taaactgttt cgttcttgac caaaaggcat atcgaacaat
    11461 ccatacttcc agcgacaaga ttgagaacat ccgccacggt ttgcatcccg catcgacata
    11521 tggttggaca atgtgcatcg accagaataa gagatacaca tggcaccatg gataaatgct
    11581 tcgatttcgg tatccgtatt tttacgaatc tctgccactt cctccattga aacttcacga
    11641 gctaaaacga cacgttccaa cccttcttct ttccagaatt ccaatgtctc aaaattagtt
    11701 gctgaagctt gagtggataa atggatcggc agacctgggg cttctgtcgc acaaatttcg
    11761 atcaaagctg gatcagaaac aatcactgca gaaataccga catcacgcaa actgcggaaa
    11821 aattcccctg ctccatcttc atttccttca tgggtgacca tattagcagc aacgtatacc
    11881 ttcgcatcat gttcttttgc aaaagcaact ccttcagcca tttcttcata agtaaaatta
    11941 cctgcacggc ttcttaaacc atatgcattc ccgccaatat atactgcatc tgctccataa
    12001 tggatcgcgg tttttaactt ttccagtgtt cctgctggcg ctaggatttc tggtcgtttc
    12061 aattttcttt ttactgtcat ttctttcatc atactcctta tttgatagca gaaggatcaa
    12121 tataaaagaa tcctgtatct aattcacggt tttcaggatg caatttactg atttcgctag
    12181 tcattttttc cgctttttct tgatcccagc taccttctaa aatttgtttt tttgcttcgg
    12241 cgaatagtcg agtgatgttt acgaattttt cgcctggagt aaataatccg tccaatttcc
    12301 atgtacagta atgatgctga taaagttcat tcaattcatt cgagagattc agatcattgc
    12361 tggcaaaaat atgtgtgcca tgactatctt cataaataga ataatgtgtc tcttcttttt
    12421 taggttccga gataaaaaga cctctttcac gatctttttg ttcgtcttgt ttcgtatagt
    12481 tatagtaatt ctgtagcagt ggacgtttgg attgatggat gcatgtagca ccatacacaa
    12541 gggtttctac aggaatatcg agtttttctt ccatcgcctt catctcttca aaaggaactt
    12601 cacgagcaag tactgctcca actgcgcctt ttttcgccca gaaattgatt tgacgtgcgc
    12661 tcgttactaa tgtttcagca tcataaatat acggtatacg caattcagga ttttttttca
    12721 tcgtataaat aatacctgta tcaccaactg tgatcacatc tacgtttatt tcttttaaaa
    12781 aagccaaata ttcaggtaca tttttcattt tttcaggatg aatgatccca tttacagcaa
    12841 tattcgcttt tttaccatac ttgtgtgcta attctaccaa ttgtcgctgt tcttctcgtg
    12901 taaatgaagc aggcagacgc aatccaaatt gctcttcacc gaaatatagt gtatctgcac
    12961 ctgcagcaag caactgttct gcttgttcaa tagtttctac tgtggttatg agttcgatca
    13021 taattttctc ctcctacaac ttcataatag tagcatagct ctaaaaactt tccaatactt
    13081 cttctattta aagtcggatt tttgaaaatt atttcacaaa aaatcaaaat aaagttcgat
    13141 atattctgtc caaatataaa aagagattgt gacaaaaatc ttgtcacaac ctcttaatca
    13201 agtcttacta ttataaaaat tcagctacac aggccaagta actataggga acctactgtt
    13261 tcctttttag gtccgcgaac attcaatgtg atcttacctt ttgaagcacc tactgttacg
    13321 tggtcaccta aacgaatttc tccgccaagc aaagcctcgc tcaatcgatc ttcaatttct
    13381 ttttgaagtg cgcgacggat tggacgtgca ccatattcag gatcaaatcc agctttcccg
    13441 atcacatcga ttgcagatgg tgtgattttc acttgaatat cttgttcttt caatcgtttg
    13501 ataatcgctt tgctcatgat ttggacgatt gtatggattt catctttact caatgaatgg
    13561 aagacgacag tctcatcgat tcgattcaag aattcaggtc ggaaagcttt ctttaactct
    13621 tctaagatac gtttttgcat ggctttgtga tctttagtaa catcttggac attgaatccg
    13681 acattttttt cttctcgaat ttgtgtcgct ccgatatttg aagtcataat catgattgta
    13741 tttctaaagt ccacttttct acctttagaa tctgttaaat gaccatcatc caatacttgc
    13801 agaagtaaat tgaaaacgtc tggatgtgct ttctccactt catctaagag aatcactgaa
    13861 tatggttttt gacgaatttt ttctgtcaac tgtccacctt catcataccc tacgtatcct
    13921 ggaggagacc cgattaatcg gcttgtactg tatttttcca taaattctga catatcaaca
    13981 cggatcaggg catcttcact tccaaacatg acttctgaaa gtgctttggc caattctgtt
    14041 tttccgacac cagttggtcc aaggaacatg aatgaaccaa ttggacgatc aggatctttc
    14101 aatccgcttc gtgcacggcg gatcgcacgt gaaactgctt tgacagcctc atcttgacca
    14161 acgacacgtt catgcagaag tccttccagt tctaacaagc gttcgctttc ttttttctcc
    14221 aactgctgta gagggacacc agtccactcc gatacaactg tagcaacatc ttcttctgtt
    14281 acacgatcag aataaccttg ctcctgtttt gcttcaattg cagaaacttt ttgcagcttt
    14341 ttacgtagtg ctttttcttg tctgcgtaat tgagcagcat tttcaaagtc ttgtttttgg
    14401 atcgctgctt ccttttcttg tactagttca tcgatttcta gtttgatcac agttgatttt
    14461 gttaagtgat cggtttgatc caaacggact ttcgctgctg attcatcgat caaatcaatt
    14521 gctttatcgg gtagttgacg agaagtgata taacgaacag aaagattgac tgctgctcgt
    14581 acagcttcat ctgtgatctc cactccatgg tgctcttcat atcttgtacg taatccttga
    14641 agaatcactt ctgcttcttc aggagttggc tcatctactt gaatgcgagc aaaacgtcgc
    14701 tctaatgcag aatctttttc gatatatttt tgatattcat ctagggtcgt tgctccgatc
    14761 gtttgtaatt ctcctcgagc taacgccggt ttcaagatat tcgaagcatc aattgctcct
    14821 tctgctccac ctgcaccaat taaggtatgc aattcatcaa tgaataagat tacttgacca
    14881 tcattataga tttcgtcgat gacctttttc attcgatctt caaattcacc gcgatatttc
    14941 gtaccagcaa ccaaagatcc catatcaagc atcatcaggc gcttttcttg catgtcttct
    15001 ggcacttcac ctaaaatgat tttttgagct aacccttcag caatggctgt tttgcctaca
    15061 ccaggttcac ccaccagtac agggttattc tttgtccgtc ggctaagaat ctggataagc
    15121 cgtttcactt ctctcgaacg accaacaact gggtccagct tgttttcttt tgctaatttc
    15181 gtcaaatcac gtgctaatga atctagcgta ggtgttcctt gcggtgtttg ccgtttatct
    15241 tgtacattta catttctacg acgattcatt ccttttccag cagaatcacc agctagcccc
    15301 attttttgtt tcagaacttg acgggctttt gaaaggctga tacctaagtt gatcatgatt
    15361 cgagtagcca tgatactttc atcttttaat aagctcatca aaatatgttc tgttccaact
    15421 ttttgagaac cagttttctt cacttcgctg ctagctaaag caaaaacttg tttcattctt
    15481 ggtgaatatg gaagaaagac tcctttaggg taagaacgga taccaccata accaatcaga
    15541 tgttcaattt cttcataaat ttccgtttca ttcaaatcca tttcacgtaa tacttttcca
    15601 gcaattccat tttgctcgac cactaaggct agtaaaacat gttctgaact aactgattga
    15661 tgtttcaacc gttttgcttc ttcttgtgca atttctaata ctgcttgggc tctttcagta
    15721 aaaagttctt ccatctaatc tcacttccca tcttcataac ttaaacgttc cagaaatgct
    15781 cggagaatcc gagcacgtgt atgatcttca tattcattga aattcaatgt gttttttgca
    15841 attgcactca acatcaagtt tccttctttt tttgtaatta tttggtcttc ataaagtttt
    15901 tgaataatag caaaagcatt tttctcactg attgtatcat caaataactg atttatctgc
    15961 tcaagtaatt gttttttatc tgaaatcctt acttttgcaa ttcgtatata accaccacca
    16021 ccacgtttgc tttctactag ataaccacgt tggatggtaa aacgggtatt aatcacatag
    16081 ttgatttgtg aaggaacaca gttaaagagg tctgccattt caatccttcg aatctcgatc
    16141 atttcactat tttccagaat tttctttata tatgattcaa ttaaatccga ggtattctga
    16201 tgtgccatct tattcattcc ctttgactaa tattgacctt aattataacg aatttgataa
    16261 tagaaagaaa gaagaacgct ttttgttaaa taaatccttt agaatcaaca aaaaaaaata
    16321 cggaatatac tattccgtat cgacgcgccc aagaggagtc gaacccctaa ccttttgatc
    16381 cgtagtcaaa cactctatcc agttgagcta tgggcgcata ttcaatttaa aaaaattata
    16441 aatcatttga aaacaaaaag caagataaaa aaaatcggga agacaggatt cgaacctgcg
    16501 accccttggt cccaaaccaa gtgctctacc aagctgagct acttcccgtt taaaaaataa
    16561 aaaagac
//
LOCUS       DAHHZJ010000048        16363 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.049, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000048 DAHHZJ010000000
VERSION     DAHHZJ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.049"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..888
                     /locus_tag="NBL02_001272"
     CDS             <1..888
                     /locus_tag="NBL02_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HCD2571967.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
     gene            complement(930..1622)
                     /locus_tag="NBL02_001273"
     CDS             complement(930..1622)
                     /locus_tag="NBL02_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCD2571968.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            1617..1883
                     /locus_tag="NBL02_001274"
     CDS             1617..1883
                     /locus_tag="NBL02_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCD2571969.1"
                     /translation="MHRTFGSRGCPTLHLADRYAERKTPTLCFAFSARTRQAGIPLED
                     IKDFLGHKDVSTTQVYAHISPEVKKRSMNQLENYIEEQIKKHSN"
     gene            complement(2059..2628)
                     /locus_tag="NBL02_001275"
     CDS             complement(2059..2628)
                     /locus_tag="NBL02_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCD2571970.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(2702..3025)
                     /locus_tag="NBL02_001276"
     CDS             complement(2702..3025)
                     /locus_tag="NBL02_001276"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571971.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVITDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(3179..3991)
                     /locus_tag="NBL02_001277"
     CDS             complement(3179..3991)
                     /locus_tag="NBL02_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571972.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            4345..5193
                     /locus_tag="NBL02_001278"
     CDS             4345..5193
                     /locus_tag="NBL02_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCD2571973.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPRPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            5338..6279
                     /locus_tag="NBL02_001279"
     CDS             5338..6279
                     /locus_tag="NBL02_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HCD2571974.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLKKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFTHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            6269..8440
                     /locus_tag="NBL02_001280"
                     /pseudo
     CDS             6269..8440
                     /locus_tag="NBL02_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(8552..10723)
                     /gene="copB"
                     /locus_tag="NBL02_001281"
     CDS             complement(8552..10723)
                     /gene="copB"
                     /locus_tag="NBL02_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HCD2571975.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(10743..12929)
                     /locus_tag="NBL02_001282"
     CDS             complement(10743..12929)
                     /locus_tag="NBL02_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HCD2571976.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(12929..13138)
                     /gene="copZ"
                     /locus_tag="NBL02_001283"
     CDS             complement(12929..13138)
                     /gene="copZ"
                     /locus_tag="NBL02_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HCD2571977.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(13151..13591)
                     /locus_tag="NBL02_001284"
     CDS             complement(13151..13591)
                     /locus_tag="NBL02_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HCD2571978.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(13665..14204)
                     /locus_tag="NBL02_001285"
     CDS             complement(13665..14204)
                     /locus_tag="NBL02_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCD2571979.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            complement(14819..16285)
                     /locus_tag="NBL02_001286"
     CDS             complement(14819..16285)
                     /locus_tag="NBL02_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD2571980.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         4793 a         3356 c         2886 g         5328 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt caacttttgg
      901 ggttcagttc acaaagaggg ggggattttt tatcgttgtg gaatatttaa ataccaacgc
      961 ttgtcatgaa aatcttgtgc tccaccctta gtatttcctt ctggatcgtt tgtcgcacgc
     1021 atcatgacgt atactttctt attagggaaa tcacgcatat tgaaagatac atgatagcca
     1081 acatttccta aagtattata agcttgattt acgtctggtc tataaatccc atcagctctt
     1141 actctagcta gttcttttcc agtattgtag tccataatga aaatatactc gtatttataa
     1201 ttagcaatgt gccatccagc tacatgcaag tttgcgtttt cgatttctcc gaactgatca
     1261 atgtgggcgt aatttgttcc atctgtcagc gtagaatttg cagcacctgc tcgagttgga
     1321 tcaattactg gtttatcctc cgaagtagtt ggattatcat ccgtaaaacc atgagccaaa
     1381 tcataggcta gtttttcttt acttacaccc atttcagaaa gataaccgta aggatctgta
     1441 tgatcgcccc aaatattttg tgttacccat aaatgcgatt tgattcctgg ttggttataa
     1501 ggagtgtcta atgttaatgg aataccatat ttcattgctg aatctcttgc caattcaacg
     1561 tatactttat agttcttttc aaaagtttct ttatcatgtg tgtgttgtaa ctcgatttgc
     1621 acaggacttt tggctcacga ggttgtccta ctctacatct tgctgataga tatgctgaaa
     1681 ggaaaacacc aacattatgt tttgcttttt cagctagaac gagacaagca ggaatacctt
     1741 tagaagatat caaagatttt ttaggtcata aagatgtttc cactacacaa gtctatgccc
     1801 atatttctcc cgaagtgaaa aaaagatcaa tgaatcaact agaaaactat atagaagagc
     1861 aaataaaaaa gcactcaaat tgagtgcttt tttaagctca ctatcactaa aactatcact
     1921 aattttattt ataagcgaac atgtttctat cactgatttt ttaaatctag cgaaaaaccc
     1981 ttatcttaaa ggcgttcgtt caattctttg gcaaggtctt caaagcctgg cttgcctaga
     2041 agtgcgaaca tggttttctt atagctcatg tagaaaaatt ttcttgatga tgattttatc
     2101 catcaattga gattgactgt tttgctctcc tatgatttca gctatgattg tccgattttg
     2161 cggttttctt cttaactggt agttgatttt cccagtttcc atcaactcgc taagcatatc
     2221 cacgactccg ccaaatccgg tctttttcgt aataaaacct agtgctacca ttcgttcatc
     2281 tagatttttt gctgtaatcg tcacaccagt aatgagcgag tgctgtaatg tttgggccag
     2341 ctcttccatc ccttcagcat tggatacgac ggaagctgtt ttgttaatag gttcttcgtt
     2401 tatttctttt ggttctttcc tctccacttt cactgtttga cgaaggacat ctagttcttg
     2461 caagagcgtt tcgtattgtt cttgggtcag catcacgcca gctacttttt cacgattaaa
     2521 tatatatacg ccagttcccg cgtctctcgc ttgagcaaat acatccatcg gcgatcgttt
     2581 cacttctgtg atcgaagatg tgggaacttc taatttcttt aattccaatg gatttcctcc
     2641 tttactaatc gctttttatt atagcacaga ctgacttgtt ttgatctttt ttccaaaggt
     2701 tttatcggtt tttgcctaac cagtaattga tatcggtaag aagtctttcg ataccaaatg
     2761 aaggatggta cagatacgtt ttttgatctt cgttatggtc tagtggaaaa aaatgatcgg
     2821 tgatcactag atctgcatac cgaggcggct tatttcgagt acatacatgt atagagatcc
     2881 gttttcgatg tcttagcgtt ttgttcagta ccccagcaag tcttcttgct tcttggtgtt
     2941 catagcgaaa aaaaaagacg attttcactt caggagggtg gacatctaaa taccctcgtt
     3001 cctggagtgt tttcagtaaa agcatccccc actctactgc ggggtgtgca agtgcttgtt
     3061 ggatcgctag ccgaaattga tatagtttat ctttggaatt gggaaatttt tctaatgttt
     3121 cctgataata acgaacttct tctatcagcc ggtcatggat accaattacg taacgaagtc
     3181 attcttggta aagtactttt gtcagctttg atttgctttc gctctcaaat tcgctattca
     3241 gccaaaacag cgaatcaagc ttttcagtca caatcgtcaa tttccgatag tcttgtgtct
     3301 cttctatccg tttccaccgc ttttcagtaa tctggttaca gctcaagcgc aaaattgcta
     3361 gataattttt ttcttttatt ggcaaaatct gctcctctct aaatagcaat tcaggaaaca
     3421 attcaaagaa tagtgttcga ctgtctccta aaatctcgat ttctgccgga tcctttcttt
     3481 gcttcaataa ttggatgaac atctcagaaa aggactgctc tccatcatct gcaaaaaaat
     3541 gcggaaaatc tttcaaaaag caaaacagca gatatcttac ttttctttct gaaccaaaga
     3601 tctgtgcttt tttataagaa aaagaaaggt ggtactcgcc aaaccacttt ctcaatcgct
     3661 cttgcgtaga acgatcgtac ttctgctcgt aaggttcatt caatagcaga tttttcagta
     3721 aacaaaacgg gggtgattgg atcagaaaaa caaacaagag ataaggttcg ctatatcctt
     3781 gatcataaac taactgaacg gttttcttat gaagaatcag ctgaatggga cagtcgcttt
     3841 ccgccaaacg tttcagatgc cgatccactg ttttaacatc gattttcagt tcttcagcta
     3901 aatcagcttt tcgccagatc aaatctttca ttagattgat tagtgaaaat aaattctgtg
     3961 cattttttga taatagtttt tgccatctca tcgatcattt ctcacctgat ttctaaacat
     4021 attgtttgat caaaatgggt tcactcctaa aaagataaaa aactcccttt ctatttattt
     4081 aaatacgcaa atcttcctca ttatttttta ttttactcaa tttaatcgta ttctcatttc
     4141 aaaacgaatg tactttaaaa aaacataata agatcacttt gcttgtagga tgtattccct
     4201 tccattacaa tgaaataaat agtgacttta tgagaaggtg atgtgaaaat ggaagaaaaa
     4261 atagaagaat ccaaacaaaa aatcgatcag ttgatataag aattagaaaa agcggtcatc
     4321 ggcaaacgtt caactatcca attgatgatc gtggccctat tagcaggtgg tcatgtctta
     4381 ttcgaagata ttcccggtgt aggtaaaacg ttactggtca aagcactagc aaaagctgta
     4441 cagggaacct tcagcagagt ccaatgtact cctgatctgc tgcctagtga tattcttggt
     4501 ttttccgtat acaattcaca atcaaaagag tttgaatttc gtccgagacc gatctttacc
     4561 acgatcttgt tggcagatga gatcaatcgt acgactcctc gtactcagtc agcactttta
     4621 gaagcaatgg ccgagaatca tgcaacgatc gacaacaata cataccctct agacaatcat
     4681 ttttttgtct tagctacgca aaatccaatc gagtacgaag gaacatatcc tttgccggaa
     4741 gcacagctcg atcgtttttt attccgacta caaatcggtt atccttcttt tgatgacgaa
     4801 cttttgctac ttttggataa attcgaaaaa gtgttggata acttaaatga agtattgaac
     4861 agttatgaaa tagaagaaat aaaaacaaat gtggcgaatg tctttgtcag tcccgaagtg
     4921 gcatcttatg ccttgcagtt agtgtcagca acaagaagtc atgctgccat ccagcttggc
     4981 atcagtccac gaggaagcct ttcctttatt caggcagcaa aagcttatgc tttaatccat
     5041 gggagaaatt acgtcacgcc caaagatttg caggatttag ttccttacgt gttttcccat
     5101 cgcctgattt tttacgatcg ttcattgaag gaagacgaaa agaaacaaat cattcatact
     5161 cttgttgcac aaattcctat tcctgtgagg tagattgagg tagacaatgg aaaaaatcaa
     5221 agattatttc attaattttg tgttatttct ggtatatttc atcattgtgc tttttgctct
     5281 tgtattcaac aattctttag gctggttctt gttggccttt tttactcttt atgtatcatg
     5341 gagcttgatt tcattaagca gctttctaaa gaaactttcg gttacttgta cattgcagcc
     5401 actcgtttat cgcaaaaaaa gtatgcaggt tattttttcc atagcaaaaa agagaacttt
     5461 ttctttactt ctccctcgtt tacaaatcgg cttcccagca atctttgcag aaaaaaacaa
     5521 gagattttga ttcttacaaa taaaccgcaa gaagtccaat tgctttggca gccaaaagaa
     5581 agaggatgcc atgaaacatt aactgtcatt tttacagcct atgatagtct aggacttttc
     5641 agaaaaagtg caaaaagaga gatctctttt cctgtgactg ttctgcctgc tttttgtaag
     5701 attcaagcag aaaaattaca gagagttctg gaaaaaaaac agcagctgaa cgcgtaccaa
     5761 agaggtcttg attttcggga acatcgcgct tatcgtccag gtgattcttt caaccgaatc
     5821 gattggaaat tgtctgctca tactcaagag tggttatacc gtgaatacga ttatcaagaa
     5881 gaagaatgtt ctcctctttt ttgcttttgg ggttcgccca gtccaaaatt tgaacctctg
     5941 ctagacttgt atttttcttt atggcatttg aaaaaagaaa agcagccgga acttctgata
     6001 ttaggcaacc gcgcctttca tggcaaagac cccggtcata cctattttgc ttctttggaa
     6061 gctggcgatg agaaagcttt tttcacacac ttgaaaaaat atgcaaaaaa acaaatcgtc
     6121 ctctttgttc ctgagaattc tcctgaagtc tcagaagcta ttgaaacatt gtcaaaagat
     6181 cgaacggttt atcttgttta tttcgtagaa aaacaactca tggtccaaga aaaagacaag
     6241 atatgttcat tgccaggagg tgaatggatt gacgattaaa cgggaaaaat ggctgcgtgg
     6301 cgtttactcc tttgtgatac taagtgtagg tgctccgata tttgtagtag ctaatcaatt
     6361 gcaaaacagc taccttctta tcctaactat tggtgtccta tgccttattg tctgctgtat
     6421 tcctaaaaaa tgggtacgtt tgctgctttc tttgccagta atgataggtt gtttgttttt
     6481 atattttcct tcgaagacat tttccatatt atggatctta cagttttttt cgtcagttac
     6541 agaggaattc ttccatctat cccaaggtaa actttattat ttgccagaaa agacagcttt
     6601 ttttctcata atgttgctga tctatttatt tattacattg accgtggatt acgactattg
     6661 gtacgcaccc tttctttctt tgatggctta cttcatgatg cttaccgttt ttcggaaaaa
     6721 agatttactg tacgaaatga ttgcggtagt aactgttttg ttcgtgtatc tggcagcaag
     6781 gcaatttttc tccatccggt tgttgtcggg aaacactcga ttccagtcat tgactacagt
     6841 tctgcttatt tgtttcttaa cttttagcgc tatcttgcct ttatatcttc cgaaagtaca
     6901 ccaatctctc gaggaaacga gtgaaccgat ccgagaatat cttaatgatg aagggcttta
     6961 tgacacactc catgagtatc aattaaccgg aagtgcaaga acaggcttca gtgagaatga
     7021 tgaacagctt ggtggacctt tatatgataa tggcgaagtt gtttttacag cgaaccaaga
     7081 aggaaatggc tattggaaga tcgaaaataa aaatcattac accggttctg gatggataga
     7141 agatcaggac gcacccatcc aaccaatcca agaattccct gttgacatca cgagctacca
     7201 aaccgttata gaagacaaag aaaacattac attgacccta aaaaaacaag ttttcttacc
     7261 ttatccttat ggaaatacta ccttgactga tttttcatta gctagtggac aaacgcttta
     7321 ctatttgcct gaaagccagc gattcattcc tgtcccgaat gaatcgacac gttctgcaac
     7381 tttacaaaca gaaaggttag tttttacgag agaagaatta aaaatcagtc agttaaaagc
     7441 tgatgattta gaaaatcagc agacaaatct tcaattgcca aaaagtttgc ctaaacgtat
     7501 tgaggacttg gcggtcgaat tgaccaaaaa tgaagcaact ttaattcgta aagtcgaagc
     7561 tgtctaacag tatttaaaaa caagcggaaa tttccgctat tccaaaacgg ataccgggca
     7621 tactccaaaa gaacacgatt atgtggatta tttcctcttt gattcaaaag tcggctattg
     7681 tgacaacttt tcaacttcta tggtcgtcct tcttcgaaca cttggtatcc cagctcgctg
     7741 gacaaaaggt tttacaaatg ggctcagaac aggcacagat aatcaaggga atgaggaata
     7801 cagcattctc aatagtcatg ctcattcttg gccagaggta tactttcctg gatttggctg
     7861 gcttccattt gaaccaaccc cttcatttgc caatccaatg ggttcacaac cagttgctga
     7921 aacaaccgaa accgtccagc ctgctatcga acaaccaaat gaaagcgaaa atatggcaga
     7981 aactatttcc agtgagagat ctgaggtcat tccatcagaa gaaacaacac tcacaccagc
     8041 ttataaaatc gtcttattca gccttggagt gtgtttatcc gtcttgttgt tctttctatt
     8101 gttattcaga aaatctctgc gctggacaag ttatcttatc tatcttcatt tttttgcttc
     8161 tactaatacg attgcatatc aaaaacttct taaccctttg gaaacattgg taccgcgcaa
     8221 acccgcagaa tctttgcagg aatatagcct gcgaatagaa caagaattca agctagatct
     8281 gcgtttcatt cgattaacta ctcaatatga agcagaaata tatggacgac aagcagatcc
     8341 ggtaaaattc gataaaaaaa cctatttaga aataacgcat ataataagaa aaatggtata
     8401 caaaaaaatt ctgacaaaag ggagccgctt caaaaaataa gataaaattt ctggagaatc
     8461 tacttctgga atgccgagaa ccagctgatt gagtatcgat tactcgaaaa aaaggttgtg
     8521 acacgttttt tgtcacaacc ttttttcatt attatctgat tttcaatgtc aaagcattta
     8581 gagctacaac taccgtactt aacgacatca agacagcacc aactgctgga cttaatatga
     8641 taccaattgg cgcaagaatt ccggctgcta gcggaattgc aagaatattg taacctgccc
     8701 cccaccagag gttctggatc attttctttc gcgtttgctt ggctagatca agaaaatgca
     8761 aaatatcttt tggatcgctg tttgtcaata cgacatccgc agaatcgatt gcgatatctg
     8821 tccctgcacc gattgccatc ccgattgaag cacgggctaa acttggcgca tcattgatcc
     8881 catcacccac catgattact tttttccctt ggtcaaggta tttctggaca acagcttctt
     8941 tatcatctgg taataaaccg ccataatatt cttcgatccc taaataatct gctactgctt
     9001 gggcagcgtt tttattgtca ccagtaagca tgacaggagt gatttttctt tctttcaatg
     9061 ttcggataaa ttcttttgct tctgttttta ctacgtcacc taaagcaacg attgcagcta
     9121 gatgattttc taaaatcagg aagctgatgg tatttccttg ttcttggtaa ggtttcaata
     9181 atgcttgttc tacgtttaat ccgagacgct caacttcttt ttcattaact attttcactt
     9241 gttgtttttt gactgttgcc tctagcccga ctcctgaaag attttttagg ttttcagctt
     9301 gataaggctt gatttcatgt tctttcaaat aattcataat gccaacagcc aatggatgat
     9361 tggcattcgc ttccaaggct cctagatatt gaaggatttc atttttattg aacgcatcat
     9421 caagtacttc gacacccgtt acagtaaatt ttccttctgt tagtgttcct gttttgtcca
     9481 gcatgatcac atctagatca ttggcttgct ccagtgcatt gcggtttttc aaaagtaatc
     9541 cgtttttagc tgctattgat gtggaacgag caacaactaa tgggatagct aaacctagag
     9601 catgaggaca ggcgatgata aagactgtta ccatacgttc taatgcatta ggaagatctg
     9661 tcaagaacaa ccaggcgatg aatgccagga tacccacaat taatgcgaca taaaacagcc
     9721 attttgccac tttgtcagat aatgactcta gcttggattt ttctccttgt gcttttcgca
     9781 ccatttccat aaccttcgct agataaccat tttcaccagt tccagttact tcgatttcga
     9841 tcgttccatc cccattgata gaaccgccaa tcactttgtc attggcttgt tttttgattc
     9901 ctcttgattc gccagtcaca gctgattcgt caacaattgt ttcacctttc aagattacac
     9961 catctgttgg cattttatca ccagaccgga cgatcaaacg atcaccttcg tttacttctt
    10021 tcaatgaaac tgtctcttct tttccatctt tcgtcaagcg tttcactgat tctggcagta
    10081 attctgctag tttttgtaaa gcattgctgg cattcgacac agcattcatt tcaatccagt
    10141 gacctagtag cataataacg atcaaagtag ccaattccca gaagaaatcc atgacgtgtg
    10201 tatgtggatt gagcagattt gcgataaacg aatagacact ataaatatat gcaactgtaa
    10261 tccccatcgc gatcagtgtc atcatagcag gacttttttg tttcaattcc atcttagctc
    10321 cacttaaaaa tggctgaccg ccatagataa atagtatagt tgctaagatc aatacgagcc
    10381 aatcagaacc aggaaacgtt acttgaaatg gaaattccat tcccatcatt ggtgaaaaaa
    10441 gaatgattgg gatagctagg ataagcgata gccaaaattt ttgtttgaaa ttccccatgt
    10501 gcatggaatg atccatgccg ccgtgttcgt gatccatgtg catcgaatgg tccatttgtt
    10561 tgctgtttgc tatatctcca tggttatgtc ccatgtgcat ggaatgatcc atctgactat
    10621 ggtcatgttt catctccatc tgtttttcat gagattctgt actttctttg tttactactt
    10681 cttttttatg ctcatgtttt ttctccatat cgttcccatt catttttact ccatcccttc
    10741 tcttactttt tcatgtgtcg atttaagctc agggagttca acaatacact gacggaacta
    10801 aacgccatcg ctcctcctgc aatgattgga ttcaaaaagc ctagtgcggc aaaaggaata
    10861 ccaatcgtat tatagacaaa agcccagaaa agattttgtt tgatttttct caatgtcaac
    10921 ctagacaact cgattgtttg tgcaatggat gccaaactgc tattcatcag cgtcacatcg
    10981 gctgtttcca tcgcaatgtc cgttccactt cccattgcca taccgacatc agctaatgct
    11041 aaggccggtg catcattgat tccatcgcct gccataccga cttttttgcc atctttctga
    11101 agtttttcta cgtaggctgc tttatcctct ggaagtactt cagcaaagat atgctttgga
    11161 tcaataccaa cttgtttacc aatcgtttcg gctgccagtt tgttgtctcc tgtcagcata
    11221 aaaacatcta atcctttatt ctggagctgc ttgattgctt gtttgacttc tagtttgatt
    11281 tgatcagcca cggcaatcaa cccgatcact tgcttttcat cagctaaaaa catgactgtt
    11341 tttccctgct gttctaattc taacgcgtgt tcttggtatt catcaaatga taggttcaat
    11401 tcaattaatc gtttacgtgt acccgcaaaa tagcgtactc cgttaatcgt accgctgatt
    11461 ccagcacctg ggtgggctgt aaaatcaatc atatcgtatg ttgctgcttc ttgcagtttt
    11521 ccgtattcca cgattgcttt acctagtgga tgttcagaag cttgttctaa agtataaaaa
    11581 atagatagga cttctttaga accgactaga tctgtaactt tgggctttcc ttctgtaatc
    11641 gttccagttt tatccaaaac aatgctgtct aagtgagcag ctgcttctaa tgcttctccg
    11701 cctttaatta aaattccatt tcgtgcacca acacccgttc ctaccataat agctgttgga
    11761 gtcgctaaac ctaatgcaca tggacacgca atgactaata cagagacact atggatcaat
    11821 gcgagttgcc aatcaccagt gatcaaacct gtagctatga gcgtgataaa cgcgatgact
    11881 aatacgatcg gaacaaaaat ccctgagatc cgatctgcga ttttttggat tggggctttg
    11941 ctcccttgag catcttccac catttgaatg atttgcgcta atactgtctg cttgccgact
    12001 tgtgtaactt ccatgtgaag tagaccattc gtattgattg tcccgccaaa gagcgtgtca
    12061 tccggattct tttctacagg aagattttca ccagtaagca tactttcatc tatcgcactg
    12121 cttcctgaaa taattcgacc atctgctgga atctgctcac caggacggat gactagttga
    12181 tcaccaacga ctacttcttc aatcgctaac gtttcttctt tcccgtttct tatcacttgg
    12241 gcggtttttg tttgaagaga catcaattgt ttgattgcat tacctgtttt cgtttttgct
    12301 gtatgttcca aatatttgcc gagcaaaatc agcgtgatga tcatactgct gctttcaaaa
    12361 taaagatctt gggggtgtcc acggaaaaat ccgttgtaaa tgctcaatgc aaaggcagcc
    12421 gatgttccaa ttgctaccaa tacatccata tttggtgctt tcgtcttgat tgcatggtac
    12481 gcacctttat aaaaacgagc accgacataa aattggacag gagcagacag cactaactgg
    12541 acgattggaa aatgaaaaaa gtgaacaatc gccgcatggc ttcctaacat catagcaatc
    12601 attgacaaca tcaacggcaa agtcaaaacc gtactcaata taagatcgaa tagcattttt
    12661 ttcaaatacg cttgcttttc ttctgcaatt ttttgtttgt gggcttcatc gtacaaaatt
    12721 gcaccatagc cgatgttttc tacgctttgg attaattttt cagtggtcgt cccttcatat
    12781 ttgacacttg ctttttcagt ggccaagttg actgtggcat tgaccacacc aggttgtccg
    12841 ttcagctctt tttcgatcct agcagaacaa tttgcacaag tcatacctgt aatgacgaac
    12901 gtatccattt tggtttctgc tgttgccatc aaatcacctc tgctttataa cccaatccat
    12961 tgattgcttg acaaatcttt tcggattgaa cttttgtttc atcaaacttg accgtaccgt
    13021 ttgctttttt caaatttact ttcacttttt gcacgccgtc aagtgcgctt actgtttctt
    13081 ctactcttgc tgcacaatga ttgcagctca taccttcgat tgaaaattgt tgtttcattt
    13141 tttcttcctc ctattgatgt tgtttacatt cacattgacc tgggatacag ttacattcga
    13201 tcgtatctac tggttgcttt tgttccagtt gtttcaaaat ctcattgata tcttcttgag
    13261 tcaaagtggc ttcttctact agatccgcaa tcgtctctcc aatccttttt gcacagatat
    13321 gggaaaaaag attttccgtc gcactgcgaa ctgtttccat ctccgagaca gctggatgat
    13381 aaatgaattt ttttccttct tgttctgtcc agatcacttc tttttttacc aaacgaccca
    13441 gcaaagtttt gatcgttgct ggtttccaat ccatcgaatc agctaagatc tgcgtgattt
    13501 cttgggctgt cgtctgtcca agtgtccaaa taacacgcat gatctcccac tctgaatcac
    13561 tgatctttac tggcgttttg actttttcca tttatttcac acctttcgtt tacaaatgta
    13621 atcattatct tatgaataca gattacactt gtaaacaaaa actgtcaatt atttttgctc
    13681 tctattttca atttagttaa aaattcttgt ttctctcgtc ttgttttaag gatgatcagt
    13741 ttatttcttg cattagctac ttggatcagc tgctgtattt tcggttcatt gttttcttca
    13801 aaatgccaga taaaagataa aaattctaag tattccctgt ttagtttctc cgaaaatgat
    13861 tcaggcatat ctggccgaga ttttttgttt atctttcttt ttagagaacg ccaaactact
    13921 cggtagatgg cgagataacg cggcacttta agccaaatga tcttatctgc ttctttaatc
    13981 ctttcctcta atgtagaagt ataatttcct tcaacgatcc atgacggatt agataccata
    14041 aacaattgct gttcttgcaa aaaccacttt tttgcctgaa tactgtagtc tgttgtatgc
    14101 cataaaaggt ctagcggcag tagcggaaag tcggtgattt ctttgatctt ccttgcgagg
    14161 gtcgattttc ccgacccact ttgtccaatc acgatgatct tcattcttct ccctcacttc
    14221 tctttggaat ttggttcgat cataccactt ataacgccgc aaaaaaacaa gggactgtga
    14281 caaaagtcgt gtcaaagtcc cttgtttcga ataaatggtg ttcaatcagc tgaccttcag
    14341 cattagctca aaaagcggca aaatatgagg agctattttt ccgtttgttt ctctaatact
    14401 caggtcaaat cgctgatttt acaacctctt ccttaaacgg tgttctcctg ccaacacctc
    14461 ggtcacaagt cacaatagtc aacaaacatg agaagttgtt ttttgaaatt gctccttgtg
    14521 actcggagtt aaacggcagg ctcactacct ctttcttaaa cggtgttcca aagcttgcat
    14581 ctgcggtaat aagcccagcc aaacgaaaaa tatgaggggc tatttttgtt tggctgttct
    14641 tattacttga tgcaggacag ctttttcaca acctctgatt cacggtgttc cagaagtagc
    14701 ttcttcgaaa aacaagccga aacttcacaa aaatttgaga agcaattttc gtaaattcct
    14761 tcttatttct tgaagctgac cacttctgtc acaacctctt gtaacaagta tttctatttt
    14821 aaggctgaac cactgtttct ggttcagtga tttctttgtt tttgtctcga cgagcaattg
    14881 ctggcaagat catacctaat aagatcaaga cgattggtgt gatgatattc gtaaataatt
    14941 ggaaccacca tgctttagga tcagcagtat aatctacttt tggcaccatt cctaggatac
    15001 atgcaaacgc agtgaaggca aagcaccaag caccaaagat aaaaccgatt tttggatttt
    15061 taacaaattt atattcagaa ttgaatttct tataagcttt attcaacatc atatatgcca
    15121 agaacaccca taggtaacgc attggcatga caactgagtt caagttcgtc aaccatttta
    15181 ctaattcatt catatctttg ataccaaaaa tcggcaacaa aataatgatt cctactagta
    15241 tacctgtcag aagataacca ttcaccaatg tacctttacg attacgttta cgtagccagt
    15301 ttggtacaaa ttctggatcc gcatctgcta acaagatttt tagtggtgca tcaattgaaa
    15361 aggctaatgc ggagatttgt ccaatcatgt ttgtcaatgc gtaaatgatc atcaatgagt
    15421 taccgatacc atagtaatta cctaaaattt gaaaagcact gtatgcccca tttgccatca
    15481 agtctttagg aatattatca ctagcaaaca gcatacccat tgcgactgag cctaatacag
    15541 cacaaatccc taccattgct gctaagaaga tcatcccttt aggaaattcc tttgcaggat
    15601 ttcttgtaga gttgacataa ggggatattt tttccgcccc tccaactgca aatactagca
    15661 tcgaaacggt tgtgaaataa ctgaaatcaa atgttggaat atatgttttc acttgattca
    15721 tatgtggtgt ggcaaattcc atcgttggat tgatgaacgg tgccgttaca gctaaaagaa
    15781 taaataagat cgacatgacg aacatcgctg ttccggccag tccgccaatc actttcaaag
    15841 tagaaagtcc ttttgttgat aaatataaaa aggcaagaaa tataataagt gaaataactg
    15901 ctaccatttg tactgacatc gtatttacga tatttccatt tccttggact gcccaaccac
    15961 cagcaatcag aattgcttga ggcttttgtg ccaaataagg aatatggacg acccagtaag
    16021 tccaagcggc atagtatgcc agtcgtttat tagatgtcgt attttcgacc catgaactga
    16081 cgcctccttt actatccttg aaggctgatc ctaattgtcc aacgatcaat gcgtatggaa
    16141 taaaataaat ggccaaaatc aatagccaag aggtgacaac tgatactcct tgctgagcat
    16201 agttattaac tacattcccc atgccccaaa ccatgttaaa tgcaattaac gcaacagtaa
    16261 accagcgtaa ttgtttgttg ttcattcgtt ctctccttta tgataaaaat ttgttatcca
    16321 gatgtaatca tattcaattt ttaacaaact tacaagacag tgt
//
LOCUS       DAHHZJ010000049        16356 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.050, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000049 DAHHZJ010000000
VERSION     DAHHZJ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.050"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            40..1380
                     /locus_tag="NBL02_001287"
     CDS             40..1380
                     /locus_tag="NBL02_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD2571981.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGIILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHAADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFAAYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLIIFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLTLRKGTLW
                     DKGISFFALAVLWLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1575..2018
                     /locus_tag="NBL02_001288"
     CDS             1575..2018
                     /locus_tag="NBL02_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HCD2571982.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINAMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2280..3698)
                     /gene="nhaC"
                     /locus_tag="NBL02_001289"
     CDS             complement(2280..3698)
                     /gene="nhaC"
                     /locus_tag="NBL02_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HCD2571983.1"
                     /translation="MEKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKKYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDILEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPKLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4102..5325)
                     /locus_tag="NBL02_001290"
     CDS             complement(4102..5325)
                     /locus_tag="NBL02_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HCD2571984.1"
                     /translation="MTTTLSTNQLKDLIQYRLPEYQALALAIHDHPEVSNYEFYSSAA
                     LIKQLEKEGFWVEKEVAGHRTGFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDEIGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDAALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAALSTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSEKGLASIAIAAELLANTALDLLREPKLLEKIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5357..6196)
                     /locus_tag="NBL02_001291"
     CDS             complement(5357..6196)
                     /locus_tag="NBL02_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HCD2571985.1"
                     /translation="MKKNIRFAVITFVAIIWLAGCSQGKAATTEKKETTVKLGIIGED
                     TDVWDDVKDRLKKENIHLEYVKFTDYNQPNAALADGSIDLNSFQHQFFLDNYNEEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENKKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDEHARPYVNIIVARKEDEDNKTYQKIVDAYQTEETAKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6213..6893)
                     /locus_tag="NBL02_001292"
     CDS             complement(6213..6893)
                     /locus_tag="NBL02_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2571986.1"
                     /translation="MTEFFKTYLPNVYLIPDEFIEATKQTLYMSFWTAFIGGIIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILVLVFLSQFISN
                     QLIKKVTH"
     gene            complement(6895..7968)
                     /locus_tag="NBL02_001293"
     CDS             complement(6895..7968)
                     /locus_tag="NBL02_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD2571987.1"
                     /translation="MIELRDISVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEAGK
                     IIEKGSSIQIFSHPEEELTKDFIRTATHLDQALETISAHAAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRQKALDYLIKS
                     GVKVTILQKNELDNRIKVIEGGN"
     misc_binding    complement(8027..8265)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(8308..8541)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8618..9289)
                     /locus_tag="NBL02_001294"
     CDS             complement(8618..9289)
                     /locus_tag="NBL02_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCD2571988.1"
                     /translation="MKGAIFDLDGLLVDTERTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVQAAGSPEKVQEVRAKREEYFYQQLTSGAIQLKPYAKEILTDLKERHM
                     KLGIATTTVTKRATDILDHFQLADYFDTFTFGDEVSENKPSPVPYLTALERTGLEALS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYSVEEKEKLNLLAEGKDLRTVIEMLD
                     KKTEK"
     gene            complement(9377..12055)
                     /gene="mgtA"
                     /locus_tag="NBL02_001295"
     CDS             complement(9377..12055)
                     /gene="mgtA"
                     /locus_tag="NBL02_001295"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCD2571989.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASPNGLS
                     EEQIERSRETYGENAISYGKKTPFIIEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGEYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNDTLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEAAETLDTDTINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVAPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELITGEKISEMNDHELAQVAEEHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGKFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQQVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKIPFIQSNATFAMLVITTLGIIVGSVLPFTGFGAELGLM
                     PLPGNYWLWLFITVVAYLALVTAVKKIYIRRFQELL"
     gene            12715..13152
                     /gene="ndk"
                     /locus_tag="NBL02_001296"
     CDS             12715..13152
                     /gene="ndk"
                     /locus_tag="NBL02_001296"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCD2571990.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFATMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKGADAVPGTIR
                     GDYALPGTENIIHASDSRDAATKEIARFFPPFDTNKPSEKAAK"
     gene            complement(13174..14040)
                     /locus_tag="NBL02_001297"
     CDS             complement(13174..14040)
                     /locus_tag="NBL02_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD2571991.1"
                     /translation="MYDGELIKKLRVNKKLTQTQLAEGICSKTSLVGIETNSVKKVSF
                     GTLKSFLERMNMTLAEYEWIRNQLDEPKKLKKKRRMLDRIQEDSFDPYKEIANNRRHY
                     KKTEDLYYLVLNLHMSKKTEVSETEFEFLRYECRIIEEYLMQVPEYGQFEVSILADFP
                     WIFSDRFIENNYLKIKKRMRRIAQLNNHEQYLFTFLTNLTLFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKSENHQEFFAVIEYTLGKNQRNSL
                     EKKLLAIAKPSS"
     gene            complement(14274..16352)
                     /locus_tag="NBL02_001298"
     CDS             complement(14274..16352)
                     /locus_tag="NBL02_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCD2571992.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVSKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPATLGQTEGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTIL
                     YPVTGTNAEQ"
BASE COUNT         4698 a         3433 c         2895 g         5330 t
ORIGIN      
        1 atgcattttg attgtgaatt taaaagaaag aggttattta tggaagataa aaaattgaaa
       61 aaacaactta gctcccgaca tatcaccatg ctagctttag gtggagcgat tggtgcaggt
      121 ctatttaaag gaagtggcga agcgattgga atcgccggcc cttctgtatt gatcgctttt
      181 ttgattggcg gcataatttt atatatcgtg atgaaaggtc tgggaaaaat tgttttaagt
      241 ggtggtgata cgcaccacgg attatctgga ttgattcgtc catatctagg tgctcatgct
      301 gccgatttta ccgattgggt ctactggtcc atgtggatta tcaatatcat tgctgaagct
      361 gttgcagctg cttccttttt acagttgtgg ttcccccaca tcccagcttg ggtatttgtt
      421 tttatgttgg ctgttttaac tactatcatc aatctttatt cggtacgatt attcgcagag
      481 acagaatatt ggctggcctt tgctaaaatc agtgtcatta tcctattgat catttttgct
      541 gcttatcttg tcggtcagca aatgttgggt actggtgttt tccctaccct tcaacaatta
      601 actgatcatg gtggttttac tccccacggt atgaagggaa tcatcagttc tttgcttgta
      661 gttatctatt cttacggcgg ttctgagttg attgctatca cagtaagcga agcagatgat
      721 cctaaacgtg cgattcctaa agcaatcaaa ggcgtcatgg gaagaatcat ttccttttac
      781 attattcctt tatttttgct tttgattatt tttccttgga atactttagc aggcacgaac
      841 gttagtcctt ttgtgatggt atttgaaaaa atgaatattc catttgcggc agatatcgtc
      901 aatttcgtga ttgttcttgc tctgttctcg tctatcaatt caggtgtata tgcttcttcg
      961 agaattttgt tcttccgctt gaaagatcgg aaaggttcct ctagaaaact agctgtttta
     1021 aacaagcatc aagtaccgca acgtgctgtg ctgttttgta ccagtgttct ttatctaggt
     1081 gtggctcttt cttattttgt cggtgacaaa ttatttggct atctggcagg ttctctttcc
     1141 tatacagtat tattgatctg gatcattatc agtgctgcgt cctttgtact gacactaagg
     1201 aaaggaacgt tatgggacaa aggaatcagc ttttttgcat tagcagtctt atggttgatt
     1261 ttccttggta tcctattcac gaactccatt ggggtaactg tattgaccgg tttgctttac
     1321 ctgttcattt atttcagtta tcaaaaaaag aatgatgctt tcgtcttgac gaatgaatga
     1381 aaccaataga gaattttatt ctatttatat tgaatatctg gagctattga aaaaaaggag
     1441 ctgcgacaaa aattgttgca gttctttttt ccgaataaac ggtgttcaaa aaccatctgt
     1501 gcagattaat tgattgttct ccacgctcat gacatgatag aagaaaggaa tgaagaaaaa
     1561 ggagtttgtt ttaaatgaaa aagtattgga agtttttgtt gatgattggt gtttcgacgg
     1621 tagtcatgtt tggtatgatg tatttgaatg tttatgcgct tgatcatatc ttcttcagtc
     1681 gcacacgtgt attcatggcg ttgatgatgg gtgcagtcat ggcagtcatc atgttgttgt
     1741 tcatgtggaa aatgtatgaa aacaaaaaga taaatgctat gattttagcc gctagtcttc
     1801 ttgtttttgc cggttcctta tttatggtac gaagccaaac aactgtcaac gatacggcgt
     1861 ggatgaaggc gatgatccct catcactcta ttgctatctt gacaagtaaa cgagctgatt
     1921 taaaagatcc tgaagtaaaa aaattagcgg aagaaatcat tcaggcacaa gaagaagaaa
     1981 tcgacaaaat gaaaaaaatg atcaaagaaa tggaatgacc attatccatc caaataaaaa
     2041 aagaggatgt tccagaagtg atcaacttca agaaataaga agaagctact tctggaacac
     2101 cgtgaatcag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca gccaaacaaa
     2161 aatagcttct catatttttc gtttggttag gcttattacc gcaaatgcaa gcttttgaac
     2221 accgtttatt cgtaaaaggg agtgtgacaa aagtcttgtc acactccctt tttacgtttt
     2281 tattgttcta attgcgctgt cttgcttact gcttcttgcg gtttcacaaa tttctttgag
     2341 atcccccaac ctgtaaaggc actgaccatc gaaaacacag gtgaaagcaa actgaaaaac
     2401 acgaatggca agtaagtgag tgtaggaatg ccaaatgtac ttgctacgaa acttccggcg
     2461 attccccaag gcactaagta gttgattact gttccaccat cttctagtgt tctccctaat
     2521 acagttggat caaatcctgc tttttcgtaa gtttctttaa atgcattacc aggcaggata
     2581 acagatagaa attgttctcc aataaagata tttactccga tgcttgtcat caatgttgta
     2641 aaaatcaatt ttggggtgtt cgtcatcttt cttgatactc gttccataac ggttgagatc
     2701 aacccaaatt cgatcaacaa accacctaat gacaatgtta gaataatcaa tgacacagtt
     2761 ggcatcatgc tttcgattcc tccacggcta agtagtgaat cgatttgttg gtttccagta
     2821 tttgaaacaa atcccgattc gatgacccct gccaacttcg ttaaggaagt ttgtgggttt
     2881 tgaataaaga tcattcctac tgctacagca atattcaaca aaatcgtcaa aattgccggt
     2941 acctttttcc atgcacacaa gagcattaaa aatagaggaa tcagtgacca gatcgaaata
     3001 gtaaagtttg cttctaaaat atcagtcact tcacgaatag atgaaagact tgcggcttta
     3061 cttgtgtgtc ctaaaatcat gaataaaaag aaagaaacaa caaatgccgg aacagttgac
     3121 cacatcatat gcttgatgtg tttgaacaaa tcggcatcaa caatagctgc tgctaaattt
     3181 gttgattcgg ataatggaga catcttgtct ccaaaaacgg cacctgaaac aatggctcca
     3241 acaacaaggg ctggatttag tcctagtgtt gtcccaatac cgaaaaatgc aatcccaatc
     3301 gttgacataa cagtaaaggc actccctaca gcgctaccta cgattgcaca aacaacaaat
     3361 acagaaggga cgaaccactg gacactaatc aatttaaatc caatgaccat gagggttgga
     3421 ataattcctg cttgtatcca tacagcaatc aatgaaccga ctaaaataaa aataaagatt
     3481 ggaatgatcc ccggtttgat gccattgatg atcccttcgt gcagccaatc tgtcgggtat
     3541 ttttttatca ctgcgtacaa catgaccatt ccgatagctg ccaaaatcgt gatattaggt
     3601 gaaaccttta atccgacaac gccaatcgca atgcctaaaa caatgaccat caatacaagt
     3661 gttgcttcta aaaaacttgg tgttcctttt ttttccatga taatctctcc ttttttagtt
     3721 gttcttatgt tctaaaaaag ttaagatatc ggattactcc gccacaattc atccgcgatt
     3781 cctcctttat aaaaataaaa agacctgctt tcaactaagg acgaatgact ccgcggtacc
     3841 accttaatta aaaacagatc tgtttttcac tttcaaacga ttattcaaat gtatggactt
     3901 tccatgtaat tcgtcaatta ggcttgatcg gttcacagcg accaccgact ttctgaacaa
     3961 aacacctaac cactactgcg tgaaaagttg acttgtatga agtttacctt ccataagatc
     4021 atttgtcaat gaaaaagaaa atattttttc atttttctga aaattatttt tataactttc
     4081 gcgcacaact ttatcttttt actagaaagt agcgccctct tgtttttttc ttgctactat
     4141 ttcttgatgt tcttgtttga ttttttctaa tagcttgggt tctctcaata aatctagagc
     4201 tgtattggca agtaattccg ctgcaatcgc aatagaagca agtccttttt cacttttagc
     4261 agcagctttg aattcttctg tatgtccggc aatatatgtc tcagagattg ctacagtagg
     4321 ctgtatcgtt ggaataactt ggctaacatt tcctacatca gaagaaccta gacttgttct
     4381 ttgttccgtt tctatttctt catcaggaac ccctgcttct tttgcatgtt ggaagaataa
     4441 gtcatcgaaa gaaggcgtta cgatgacatc atcgactgca ttttggaaca atccaaactc
     4501 aaaagtcgta cccgtgctta atgcagcacc tcgtacgata ttttccactt tttcatatac
     4561 tttgtctaat gtttgtcgat ttcctgcacg taaatagaaa cgtccagcag cgtagtctgg
     4621 tatgacattg gccgccacgc ctccatctgt aatgattcca tggatattga catcttttgg
     4681 caattgcaag cgtagagcgt tgattccgtt aaacacttgg atcaaggctt ccaaagcatt
     4741 tactcctttt tcaggtgctg ctgctgcatg agaagcgaca ccgtaaaatt tgatatcgac
     4801 tggatcattt gctaaagatt ctgtcgtttt cccataacga aaagctggat ggacacataa
     4861 tgctgcatcg acatcctcaa aaaaaccttc ccgaacgaag cttcccttag ctgatccatt
     4921 ttcgcctcct tcttcccccg gagtaccata cacacgaatt tctccgccaa tctcatcaat
     4981 gaattgtttc actatgctgg ctgctaatac ggagtaagta ccaaataaat tatgtccgca
     5041 tgcatgacca atacctggta aagcatcaaa ttctgccaaa aaggcaattg tcggacctgg
     5101 tttattggaa atataacgtg catcaaagcc tgttcggtgc ccagctactt ctttttctac
     5161 ccagaaccct tctttttcca attgtttgat taaagctgct gaggaataaa attcataatt
     5221 acttacttca ggatggtcgt ggatagctag tgcaagtgct tgatattctg gtaaacggta
     5281 ttgaatcaga tccttcaatt gatttgttga gagtgtggtt gtcataaaaa tttcctccta
     5341 tgctagtcgt tttttcttat tttctcccga atgtttccca cgcaggtacg cttgaacctt
     5401 ttgatgtttt ttcgattact ttggctgttt cttctgtttg ataggcatct actatttttt
     5461 gatacgtttt attgtcttca tcttcttttc ttgcaacgat gatattgaca taaggacgtg
     5521 catgctcatc gactggttct aagaagattg catcagaagc gggattaaat ccagcatcta
     5581 cagctacacc gctattgatg actgaaatat ctacatctga caaagcacgt gctgtttggg
     5641 aagcatctaa ctcagaaatg tctagatttt ttttattctc ggtaatatca gaaacggtag
     5701 gcgcttggcc ttttgccttg tccactttga tcaatccagc agtttgcaat aacagaagag
     5761 ctctgccgcc attcgtgaca tcattaggga ttgcaacttt tgctccatct ttgatatcag
     5821 ccacatcttt taacgtattg gaataaattc ccaacggtgc attaaccgta ttcccaatcg
     5881 atactaaatc agttccgtgt tcttcattgt aattgtctaa aaagaattga tgttggaagg
     5941 aattcaaatc gatcgatcca tctgctaaag ccgcatttgg ctgattgtaa tctgtaaatt
     6001 tcacatattc caaatggatg ttttcctttt tcaaacggtc tttcacatcg tcccatacat
     6061 ctgtatcttc cccaatgatc cctagtttta cggtcgtttc ttttttttca gttgtagctg
     6121 cttttccttg gctgcatcct gccaaccaaa taattgctac aaatgtaatg actgcaaaac
     6181 gaatattttt tttcattttt tctcctccat tttcaatgtg tgactttttt aatcagttga
     6241 ttactgataa attgactcaa aaaaactaag accagaatca ataatgtagc gacaatcgtt
     6301 acatctgttt ggaatcgatt atatcctctc gatatagcaa gattccctaa tcctccggca
     6361 cctactgctc cagccatggt agtcagtccg atcacattga tgatcgtcaa cgctgagacc
     6421 cgaatgatac caggtaaccc ttctaacaaa taaacacgta aaatgatgcc gattgggctc
     6481 gtgcccatcg cttctgctgc ttcgatcacc ccaggatcaa cttctagcag tgcattctcg
     6541 atttgacggg caaaaaacgg gacaacgcca aaaatcaaag ggactaaagc tgctttggca
     6601 ccaatcgtcg ttccaactaa gaaacgagta gtcaaagcta aaagagaaag caagataatg
     6661 aaagggatgg aacgaatgat attgatcaac ttatcaagta tttcgaacag cagcctgtta
     6721 gctagtaatc catttggacg tgtcactact aacgtcacac ctaagatgat tccgatgatc
     6781 ccaccaatga atgccgtcca aaaagacata tatagtgttt gctttgtcgc ttcgatgaat
     6841 tcatccggaa tcaaatagac attcggcaaa tatgttttaa aaaattcagt cattttagtt
     6901 tcctccttcg atgactttga tacggttatc taattcattt ttttgtaaaa tagttacttt
     6961 tacaccagat ttaatcaaat aatccaatgc tttttgccgc tgtttggttt ctccagctaa
     7021 agtaacgatc aaacttccta agggtgtttc ttgtaatagc tctacattac cgtataagat
     7081 attcgctgtt acttggtact tgctgtaaag atgagcgatt agaggttcgt tcgtctggtt
     7141 gccgatataa cttaattcca ccaaccattt atttgcaatc tgatcggcaa aagcagcatg
     7201 ggcactgatt gtttccaatg cttggtcaag gtgtgtcgct gtccgaataa aatctttagt
     7261 caattcttct tcaggatgac tgaaaatctg gatgctgcta cctttttcaa tgattttccc
     7321 cgcttccata accgctacct tgttgcagat ttctttgaca acttgcatct catgagtgat
     7381 caggacgatt gttagaccta gctgacgatt tagttttttt aacagtgcaa ggatctgaag
     7441 ggtcgttttc ggatcaagtg cactagttgc ttcatcgcat aacaacactt taggttcatt
     7501 tgccagagct cgtgcgattg ccactcgttg tttttgcccc cccgacagtt gtttaggata
     7561 cgcagatgct ttttcttcca gaccgacaag ttccagtagt tcattcactt tttgacgacg
     7621 ttcctgtttt gattttccag aatactttag agaaaaatct acgttgtcaa aaatcgttcg
     7681 actttccatt agattgaaat gctgaaagat cataccgatg gcctttcttt cttttcgcaa
     7741 ttcttttgga gagagctggc ttaaattcgt ctgattgacg atgacttctc ctgctgttgg
     7801 tttctgcaac aaattcatca cgcggactaa cgtactcttt cctgcacctg agtaaccgac
     7861 gatcccataa acatcccctt tttcaatcgt taaatctaca gaatctactg cctgtatctc
     7921 tttttctttt tgtttgaaac atacagaaat atctcgcaat tcaatcatta acattcctcc
     7981 ttgtctataa aaaaagcatc ctcaagaaaa tgaaaatagt cattttcttg aggacgcctt
     8041 tttgcgtgtt accacctcat gttcaagctg tgttcacaca caactcctct tcaagtacca
     8101 tcatactcta gcaagatatc ggtcgcagcc gtcaccgcct tacatctatg ctcgggatga
     8161 attgctcgat ggccattttc caatagtcgt tccttgcttc ttctcagcta ccgaagctct
     8221 ctgtcaaagg ttcttctatc gtacttacca tttcaatgca aataatattc aaaaaaaaaa
     8281 tccttttact aacataaact atgtcagtaa aaggacgacg tatcgtggta ccaccttttt
     8341 tcggaagttt ccttccctca agcccaacat ctcaaaaaga gagattggcg ttcgttttaa
     8401 cggtcgaaca tttccgaatt tgcctactta tcgacaaatt attctccaag gccattttca
     8461 gcttttctta cttgactctt cacaccagcc agagcctctc tgcaaagttt ccaagcttac
     8521 tttccttctc aacgaaatca atattttgtt gttgggaata atctacagga ggaaaactga
     8581 aaagtcaagc atgaaatcca aattattttt tctatattta tttttctgtt tttttatcta
     8641 acatttcaat aacagttcga agatcttttc cttccgcaag aaggttcaat ttttccttct
     8701 cttcgacgct ataatttcga acaaaactgg aatcgggaat caaaacaacg cctagtcctg
     8761 cacgtgaagc ggctgtcgct cctactaaag aatcttccac tgcaaaagct gaaagagctt
     8821 ctaatcctgt tcgttccaaa gctgtcagat aagggactgg tgaaggcttg ttctccgata
     8881 cttcatctcc gaacgtaaac gtatcaaagt aatcggctaa ttgaaagtga tccaagatat
     8941 ctgttgctct tttcgtaaca gttgtcgtag caatccctag tttcatatga cgttctttta
     9001 agtctgtcaa tatttctttt gcatagggtt tcaattggat agctccactt gtcagctgtt
     9061 gataaaaata ttcttcacgc tttgctcgga cttcctgcac tttttcagga ctgcctgctg
     9121 cctgcactaa aatatcaaaa ctttgtttcc agttttttcc gccccaagcg tcaacgattt
     9181 ctttcgggat atgaatatca aattgctgga atccccaaag ccaaccatcc cgatacgttc
     9241 tttccgtatc cactaacaaa ccatctaaat caaatattgc acctttcatc tatacattaa
     9301 tcctttccaa aacaatacac ctagtttagc atgactgatc caaaatgaac agaagcatag
     9361 agcccagccg catcacttat aagagttctt ggaatcgacg gatatagatt ttcttcacag
     9421 ctgtcacaag cgccagataa gcgacgactg ttataaacaa ccataaccag taattgccag
     9481 ggagcggcat caagcctaac tcagcaccaa atcctgtgaa tggaaggact gaaccgacaa
     9541 tgattcctaa tgtagtgata accagcatgg caaatgttgc attgctttgg ataaatggta
     9601 ttttcggtgt acgcaatgca tggagtacta atgtttgtgt ccacagggat tcgacaaacc
     9661 atcctgcatg gaataatgcg ataaaaatca cttgttgtga gctatccaat gtatggaaac
     9721 ttccaccaac tacagcagga caaataacaa aatacatcaa caagtaagta gtgatatcaa
     9781 aaatcgaact tgttggacca aaataaacca tgaattttcc aatactagat gcattccatt
     9841 tctttggctc atccaaatat tctttatcca tccgatccca tggtaaggaa atgcaagaaa
     9901 cgtcatagat cagattcaaa aatagaagtt gcaaaggcaa cattggtaaa aatggtaaga
     9961 acgtactggc aatcagtacg gaaaacatat tgccaaaatt agaacttgct gttgccttga
    10021 tatatttcat gatgtttcca aaagtttctc ttccagaaag aaggcctctt tccaaaatcg
    10081 tcaaatcttt ttctaaaagg atcacgtctg ctgattcttt tgcgatatcc acagctgtat
    10141 ccacggaaat accgatatca gccactttca ttgctggagc atcattgata ccatctccta
    10201 aaaatccgac agtatgccca tttttacgta aagctgtcgt caatcgtgct ttttgctgag
    10261 gattcaattt tacgaatact tcgtgttctt cagctacttg cgctaattca tgatcattca
    10321 tttccgagat tttctcacca gtaatcaatt cattgatttc taggcctact tgacggcaca
    10381 cagaccgtgt gactaattca ttgtccccag tcagaacttt tacagctaca ccatgttctt
    10441 ttaaagcttt caacgcttgt tttgttgttt cttttggtgg atcaaggaaa gcaagataac
    10501 caatcagaac catgtctgac tcatctttga ctgaaaattc tccaacaacg cttggattgg
    10561 tcttttgtgc gacaccaatc actcgtaagc cgtcttcatt aagtgattgt acttttgcaa
    10621 gcaccgcttc ttttcgttct tttgttaatg gtgcgacagt accaccaaga tcaataaatt
    10681 tagaaatgtt caacatttct tcaatcgcgc cttttgtgat catctgtgtt ttgccagccg
    10741 gatcagcgac tacaacactc atacggcgac gttcaaaatc aaaaggaatt tcatctactt
    10801 tttgataatt gattgtatct gtatctaaag tttctgctgc ttcatcaatg atagccacat
    10861 ctaacaaatt tttcagccct gtttgatagt agctgtttag ataagcatgg cgcaaaacac
    10921 gatcatcttc atttccttca caatccatat gatattcaag aatgattttg tcttgtgtca
    10981 atgttcccgt tttatccgta cataacacat cgatcgcacc aaaattctgg atagcattta
    11041 aatttttgat gactgttcct tttttggcca tcacacttgc tcctttgacc aaattcgtcg
    11101 taacgatcat tggaagcatt tcaggcgtta aaccaactgc cacagataat ccaaaaagaa
    11161 aagcttctaa ccaatctcct tttgtaagcc cattgataac gataactgct ggagccatca
    11221 atgacataaa tttgatcagc agcatggatg ttttgtggat gcctaattca aaacttgatt
    11281 ggatgggttt ttcagatagc gtttctgcta catgcccaaa tagtgtgtca tttccgacag
    11341 aaacaatgat tccttccgcg cttccactga tgacattgct tcccataaag acaagatttt
    11401 cataacttgt ttcactttgc tgatcgtctt cacatacttt cgcatgcttt tctactggat
    11461 agctttcccc tgtcaatgct gcttgagaaa tgaatagatc ctttgcggac aataaacgca
    11521 tatccgctgg tatcatatct cctgctgaaa gccgaacaag atctccacag acgatttctt
    11581 ccataggaat ctctgtatac tcgccatttc gtttgacagc tgccgttact ttcacaagtg
    11641 atttcagttt ttcagctgct tgattagaac gcacagactg gatcaacgtc atcgtcccac
    11701 tgacgataac catagtgaag ataatggcta caccaaagag atctttatct cctggagcgg
    11761 ctaatacgta ttccgtaatg aatgaaataa ttcctaatgc aatcaacact aatgtaaagg
    11821 gtgtgatgta tgctttcaat atttctataa tgaatggtgt ttttttgcca tatgaaatcg
    11881 cattttcacc ataagtttct cttgaacgct cgatttgttc ttctgacaat ccatttggtg
    11941 atgcatggaa caattcaaat aattcttgct tcgtttttcc agcaaaagct gcatagattt
    12001 tttgtgtatc tttctttttt tccatctcga tctctccttt gtgtccaaaa gacagctgtt
    12061 ctcgctgttt ttggtctttt gctattcatc ggatcgatag agaaaatgct gaaaggggct
    12121 gagaacagat agataatagc cctggccttt cctctaaatc cttgtttttc gaatgactgt
    12181 catccatttg ttctcatctc ctttactaag taattttaat tcatgacttc caatcatttt
    12241 tcgaatagtt attttcccaa ctcgctcacc tccctttttg ttttttcacc tatgggattt
    12301 gcggcaacaa aaaaacctct cgccaaatac tcagcgaaag gtttgaaaaa gcaaatcaaa
    12361 aaaagcttct cagatctcat cgttgagttt tgacactgta caacgtaaaa agcagatatc
    12421 tgcttttagc taccttatga aaagccttat ctcgacaatt cctgttctac ccattggcgt
    12481 ctctcgacgt ttttgggcag tagcctatgt ttgtatagga gcctcaccta acaaatccta
    12541 ttcggttttc cctcgttaat ataacaacta aaaaagagga tgtcaaatca attttccatg
    12601 tttttcatac ttttttaaaa tacacgcgtt atatttcctg tctcacgcag ttttatttgt
    12661 ctcattttcc acctcccgct ataatagaat tatagtagga aacgaggtgc tgttatggaa
    12721 cgaacacttg ttattatcaa gccagacggc gtaagacgcc atttggtcgg aagcatcatc
    12781 caacgttttg aagcaaaggg actggccatt gctgaaatga aatttgcaac gatgacacct
    12841 gaactagcaa aagaacatta tcagcacttg actgaacgta gtttttttga tgagcttatc
    12901 gattacatga cttctggtcc ggtcgtttat cttgtgctgg tcggcgagga agtgatcgat
    12961 atcgtccgta agatggtcgg agcaacaaaa ggggcggatg ccgttccagg tacgatccga
    13021 ggagattatg cactgccggg gactgaaaac atcatccatg cttctgattc ccgtgatgct
    13081 gcaacaaaag agatcgcccg ctttttcccg ccattcgata caaacaaacc gtctgaaaaa
    13141 gcggcgaagt gatacaaatc ctaaactgtt ggtctaactg ctgggtttag caatggccag
    13201 cagttttttc tctaaagaat tcctttgatt cttccctaat gtatattcga tcactgcgaa
    13261 aaactcttgg tgattttccg atttttcttc ccctaaagca gcagaaatca gtcgttggta
    13321 ataatccgcc atcaatgttt cataaatcac tgtacttctt tctttatgcg ctaaactccg
    13381 tttcatatct tcattcacgc tacgtgcctt ttttaagcga ccattttcta tataaaacaa
    13441 agtcaagttc gtcaaaaaag taaagagata ctgctcgtga ttattcagtt gcgcgatccg
    13501 tcgcattctt tttttgattt tcaaataatt attctcaata aaacgatcac tgaatatcca
    13561 aggaaaatct gctagaatag atacttcaaa ttgcccatac tctggtactt gcatcaaata
    13621 ttcttcaatg atacgacatt cataacgcaa aaattcaaat tccgtctcag atacttctgt
    13681 ttttttggac atgtgcagat tcaaaaccaa atagtacaga tcttctgttt tcttatagtg
    13741 cctgcggtta ttagcaattt ctttgtaagg atcaaagctg tcttcttgaa tccgatctag
    13801 cattcgtctt tttttcttta atttttttgg ctcatcaagc tgatttcgta tccattcata
    13861 ttcggcaagt gtcatgttca ttcgctctaa aaatgatttc aatgttccaa acgatacttt
    13921 tttcactgaa tttgtctcga tcccgactag cgaagtttta gaacagattc cttccgctaa
    13981 ttgggtctgt gtcagctttt tgttgactcg aagcttctta atcaattctc catcatacat
    14041 aatagttctc cttttttaat tgaaattaac ccttggcttt tccagaatgc ttgccaataa
    14101 gaggaatgat tatttcaatt taaattttcc gcattcaata aatagatacg ttaaaaataa
    14161 aaaatacacg aaaaaaataa aaaaatgttt ttctcataaa aaataccaac cttcaagcat
    14221 gagactgctg tctaatccct gaaggttggt ttgattgttt attgatcatt tttttactgc
    14281 tccgcattgg ttcctgttac tggatatagg atagtcgttg ttttattgcc gttttttgaa
    14341 aagacactgg tcgaatgttc acctttttgc ttttcgattg cttcattacg ttccgcttcg
    14401 tttttatagt catatttttt cggatcaatc gtctcaaatc cgtttggcac ataaaaccgc
    14461 aagagatttt cctgattcag cttatctgaa agacttaacg cagtcttgac tttttcctga
    14521 tctttggaga tttcctcgtc gattcctgct gctttcgtat cgatgatttc tcctgtctga
    14581 ttattgtaag cttttcctcc taggacggtg tatttgggcg tcacgaagtt cccatttcga
    14641 aaagcaacta cttgatcgtg ctgagaagat aataagtctg taccaaaatg aagataatct
    14701 ttatcatcga ttcccaatag atgtagtaat gtcggcaaaa catcgatttc tccaccatat
    14761 tcatgattga ctgtcccttt agtgtaacca ggcatatgga tcatcaatgg cactcgttgc
    14821 atctgtgcgt tgtcaaattc atcccatgta tctggatctt taccaagagc acttgctaaa
    14881 tctttattat ctgtattcga gatcccaaaa tgatccccgt aaatcacaaa gattgaattt
    14941 tggtaaatac cagatttttt caaatacgta aaaaattgtt ccaatgattg atccagataa
    15001 tgggccgttc gaacataatt atctactgtg ctattgccag tattcaaaga tggaaagtcg
    15061 aaattcttgt catccgtata ataaggcgtg tggttggtta cggaaagaaa tttcgtgtaa
    15121 aacggctgtt gtagatgttc aagatattgg gcagattctc tgaataaatc tttatctaat
    15181 atcccataac ctaacgtttc atcggattca tcaaaataag atcgatcaaa gaaattgtca
    15241 taccctaaat ttttatatac atgatctcta ttccagaaac tagctacatt cccatggaaa
    15301 acagcacttg tatagccttc tgtttgacca agggtcgctg gtgctgcttg aaagacatta
    15361 tctgacccaa gttctgtgaa taatgagcct tgaggtaaac caaatgtgcc agtttccaac
    15421 atgttttcag catcacttgt ctttccctgt cctacttcat gaaagaaatt gtcaaaactg
    15481 atcgttgctt gattctggaa aatactattt aaaaacggcg tgacttcttg tccgtctacc
    15541 ttcatattga tcaaaaactg ttgaaaactt tctaaatgaa ggacaatcac attttttcct
    15601 ttagcaatcc caaatgtttc aggatttggc tctgcataat gtttctttgt atagtctaaa
    15661 acttcatcaa ttccattact gcttgcatgc gcgcgaacac tgctagtcat tcctgttttg
    15721 attccgtcat aaatagtgta cgcatcgatt cctagatatt tgacaatata ggaacggtca
    15781 aatgtccgct gtagcaattg gggacggttg gcttcgctca acgctaaatt tcctgaaaaa
    15841 atcaatcctg aaagacaggt aacagcgata gccatcggtt tgctgacagg gttgcttttg
    15901 ataggtattt tcttgacctt caaccaaacc agtagcccaa tgaacacagc gatatccaac
    15961 caatagatga catcatgggg tttcatcaaa gaaaaggagc ttcctgacaa cccttgtgac
    16021 actttcgaaa agcctaatac agatttgact gtaatgaaat ctgtgaattc ccgataaaaa
    16081 ataatattca agtacaacaa aagcgtattt aaaatatcaa tgatcaaaag aatggctaac
    16141 gctggctttg gctttttaat atacaaagaa aggccaaaaa acaataaggt agtcgcaatc
    16201 gggttgatcc acaagataaa gtactggatc gtaccttcca cacctaatga aaaatctgca
    16261 tagtaggcaa tgatcgtctt cagccagaaa aagaaaacag ctgtcaaaac gattgctaaa
    16321 cgattttggc acaatttttt aagttgtgtc acgtat
//
LOCUS       DAHHZJ010000050        16064 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.051, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000050 DAHHZJ010000000
VERSION     DAHHZJ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.051"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            226..894
                     /locus_tag="NBL02_001299"
     CDS             226..894
                     /locus_tag="NBL02_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HCD2571993.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1324)
                     /locus_tag="NBL02_001300"
     CDS             complement(1094..1324)
                     /locus_tag="NBL02_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2571994.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1451..2833)
                     /locus_tag="NBL02_001301"
     CDS             complement(1451..2833)
                     /locus_tag="NBL02_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HCD2571995.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2989..4176)
                     /locus_tag="NBL02_001302"
     CDS             complement(2989..4176)
                     /locus_tag="NBL02_001302"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCD2571996.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4183..5214)
                     /locus_tag="NBL02_001303"
     CDS             complement(4183..5214)
                     /locus_tag="NBL02_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCD2571997.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5230..6684)
                     /locus_tag="NBL02_001304"
     CDS             complement(5230..6684)
                     /locus_tag="NBL02_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD2571998.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            6859..7764
                     /locus_tag="NBL02_001305"
     CDS             6859..7764
                     /locus_tag="NBL02_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCD2571999.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7855..8532
                     /locus_tag="NBL02_001306"
     CDS             7855..8532
                     /locus_tag="NBL02_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCD2572000.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8529..8699
                     /locus_tag="NBL02_001307"
     CDS             8529..8699
                     /locus_tag="NBL02_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572001.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8813..9709
                     /locus_tag="NBL02_001308"
     CDS             8813..9709
                     /locus_tag="NBL02_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCD2572002.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9764..11134)
                     /locus_tag="NBL02_001309"
     CDS             complement(9764..11134)
                     /locus_tag="NBL02_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD2572003.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11372..12118
                     /locus_tag="NBL02_001310"
     CDS             11372..12118
                     /locus_tag="NBL02_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCD2572004.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12115..12954
                     /locus_tag="NBL02_001311"
     CDS             12115..12954
                     /locus_tag="NBL02_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCD2572005.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12955..13905
                     /locus_tag="NBL02_001312"
     CDS             12955..13905
                     /locus_tag="NBL02_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HCD2572006.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14046..14798
                     /locus_tag="NBL02_001313"
     CDS             14046..14798
                     /locus_tag="NBL02_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572007.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14817..15968
                     /gene="queG"
                     /locus_tag="NBL02_001314"
     CDS             14817..15968
                     /gene="queG"
                     /locus_tag="NBL02_001314"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCD2572008.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5209 a         3002 c         3231 g         4622 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt tgataccgtt
     1381 gactagttct tccatcgcaa aagcagtccc ccattttaat ccaaatggcc gatctcgata
     1441 ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc ttttccacct tctaatccac
     1501 tcataccacc tgcatgcccg ccaacgagag aactacgtgc gatggcgcgt cctccttgca
     1561 agagactatg cgcatggatg attgattgaa agccgtattt ttttctaatc gtatcgacaa
     1621 caaaatcaac tttcgcttca tttatctgct tttcaggatc ttcaaaaagg ttaagctgta
     1681 aacctgtcgc gtatacgagg tcggaacagt tcacgcctac acgccgaata tcctggtagt
     1741 ggtaatgctg gtggaaaatg gtcaataaag catctgctag atctctgcta gcatttgtcg
     1801 cttcgatttt catttgctgg tgaaacccgg tcttgccatt tggatcaaga tagcctaaag
     1861 agtatcctac ccataagctg actacttgtg tttttgctgc agatcgtctc aagcgagtgc
     1921 cgacttggtc ggccatttct ttgataacga tctcgatttc ttctcgtctt gtataatctc
     1981 tatctaggat ttgactattt ccgatacttt ttgattttgg tgtatattct tgtcccaaaa
     2041 aacttcgatc gatgccccaa gcatgggcat aaagctgagt accgatgacc cctagcttgc
     2101 ttttcaagtg ataataatcc gcgtgagcta aatcataaac agaatgtata cccatttgtt
     2161 tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc agtgatatct gagattttcc
     2221 ataacttttc tggtacatct tcataacgcc attcagcaaa catttgcttt ccgtcttttg
     2281 agccattgtc taaggctagt ttagcaagta gcgggttatc gccaattcct atagtcgtgt
     2341 agattccggt ctgctcaaac acatctttat ggatcagttt agccatgtcg tacgcatttg
     2401 ttttaccaaa gagtgccatg ctatttgtta catcaaggaa gctttcatcg acggaataga
     2461 catgatggtt cgcttcgtct gcatattttt tatagataga attgatttct ctgttctttt
     2521 ccatataata ggccatcctt ggggcaacaa tatataaatc acttggatag gggaagggaa
     2581 gatctctggc acggctgata ttggtgatgc cgtaagcttt ttttgctgca ggactggaag
     2641 ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga cggataggac atgactacta
     2701 atttagttgt aaggggatca agatcctttt caacgcactc tacggaggcg taaaaagatt
     2761 tgcagtcaat acataaaata tctcttgaag gttctttttc ataaacaaat accggatttt
     2821 gattcgccca cataacaatc acctcttcaa cacaattaga acacacgttc tctttccgtg
     2881 tcaataaaaa acagcaattt tcaaatacga gcaaaaggct cacatttgga aattgctgag
     2941 aggttatttt tatctatttg tttttagctg agatcgttga tttttttatc aaacatgaac
     3001 agcttctgga tcgatctttg ttgtcccatt tccaatgtgt gcaacataaa attccggagc
     3061 atagccgaca actttcatgt attcatcata gacattgttt ttgaaggcag gtatattttc
     3121 ttctttgaca agagcaatcg cacagccacc aaatccagct cctgtcattc gtgcacctaa
     3181 gacaccttct tgtttttgag cagcatctac tagtgtatct aattcgatcc cagttacttc
     3241 ataatcatag cgaagagagt ggtgagaatc atttaacagt tgaccaaatg cttctaagtt
     3301 cccagcttct aattcagctt ttgccttcaa agtccgctga ttttcagaaa cggcatggcg
     3361 agctctgcgt atcaatgtat catcgccgat ttggtcaata gttgattcaa attcttcttc
     3421 atctaagtct cctaaagaag agataggcaa agttttttgc aaacgtgcca aggcttcttc
     3481 gcattcgcta cggcgttcat tgtattttga atctgccaat tcccgccgct tattcgtatt
     3541 catgatgacg attgcataac catctagttt cactggaacc atttcgtatt tcagcgtatt
     3601 tgtatctaat aagattgcat gatctagctc accgaagccg atggcaaact gatccatgat
     3661 acctgaattg acaccgatga attcattttc tactttttta cctgtttgga ctagttccaa
     3721 gcgatctgtt tttaaatcaa acagatcttc taatacaaca cctactaata attctaatga
     3781 agcgctactg gaaagtcctg caccattagg gattgttcct tccactaata actcaaaccc
     3841 atgatcgatt gagtgtccag cttcttttaa tttaaggacc attcctttta cgtaatttgc
     3901 ccagtttgct tttttatcat attctagctc atctaaagta aaactaatga caccgatatc
     3961 gtcaaagttt gtggaataaa ctaatatttt attatcgtct cgaggtgctg ctacaccttg
     4021 cgttccataa gtgatggaag cggggaagac atggcctcca ttgtaatctg tgtgttcacc
     4081 aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt tttttaggtc cgaatgtttc
     4141 ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg agttattcct ttcttgtttt
     4201 gattttgaat tctgttactg tttcaaatac tgatccggct tttaaatgaa tcgaaccaaa
     4261 tgctgagaag cgctctgcac ctggagctgt ttgagtctca aatgtgatgc caccgtgatg
     4321 tgccagtttt cggttgcgca tttctggtgt ttctgtgccg aaatttgcag tgaaaataac
     4381 cacactagaa gcatctgtag caatatctac ttggattctt ttatcggggc tagtcaagcg
     4441 agcggcttct ttacccaagc cggtttcttt taagaaaaat ggatgatcga ttccgtcaac
     4501 aagatttttt tgatccaaat cactagcaaa aacggacgct aattttttag gtatttgaaa
     4561 atcgaaagca cttcctgcag cattttcttt tacccctata ggaatactat ctgttcgcaa
     4621 aggagcataa gcttcgctat tgagccaaag ctcatgttga tcgattgact gagaagcatc
     4681 tcccgtcaag ttgaagtaaa catgatttgt cggattgaat aaagtatctt ggtcactggt
     4741 gccgcgagag gtcactcgcc agatattatc ttttgtcaaa gtatagcgga tttccacatc
     4801 cattgtacct ggaaaaccat gttctccgtc gggacttgta gtagtaaaaa tgacagaagc
     4861 ttcattttcg ccattcaaaa tgacataaga ccattttttt aattcaaatc caggagcacc
     4921 accatgcaga ttatgtccag tttttggatc tgtggctaat tggtaagttt ttccattgag
     4981 agaaaagcgg ccattttcga ttctccctgc ggtgcgaccg atagaagcac cgatatacgg
     5041 atctttttcg atatattctt cagcggtgtc aaatcccaaa actaattctc gatcatttga
     5101 ttttaaactg acgattctag cacctaaatc agagatagaa agtgctagct tgtttttgtt
     5161 ctccaaggtt atcaagtgat aaccttggcc aaaatctttt tctgtaatct tcattttcaa
     5221 gatcctttct taatgggaag gaactactgc atcgtgaagt tcatcttcca ttgtatcagc
     5281 tggagccaat ttgttttcca atttctcaac gatttgtgca tgttttttct ctgtcaatgt
     5341 aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa gcagcaggag catagaatgc
     5401 ataagctttg aaagtcgtta cgccatgagc agtgatatca gcagcagtag cattaccagt
     5461 catcccagaa gcgatagcga caaaccctac gatcccgtta gacaaagcac cagcgatttt
     5521 atctagtaaa gggcgaattg atagagtaac agcttcgtta cggataccat ttttccattg
     5581 accatattca acagagtctg tgatcgtcat caaagcggat aagaaaatca attgttgagg
     5641 gaagtagaac aatactagcc cgatgataac gattggcaaa cttgaacctg cgatcgtaaa
     5701 gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg taaacgtagc gacgagagat
     5761 gacgcgtgta agcattggga atagtggaac agctactaaa ccaagtaagg cagcaactag
     5821 accaacgatc caaaattcag ttggttggcc aagtacatat ttgaagaagt agaataatac
     5881 ggcagttgtt gctacattgg caatcgcata agcgatataa gcaagggcaa gccacatcaa
     5941 ctgatcattt tgagcaactg ctttgaacac atctttgatc ccagcttttt cattttgctg
     6001 acggatcgca gtatcgactt cttttgtgcc caaggcagtg ataatcgcag taccgcctga
     6061 aataacagca gcaatcacac cgaaccagaa ccagcctgaa gcgccttcaa tatgtttatg
     6121 agtaacaaga taggtaaaga aagtaacgat agggacaacg actagagtag taccatttgc
     6181 acctagagaa gagccgaaac gagcaaacgt agcaaatttt tcacgttcct ttgtttccgt
     6241 actcaaagct ggaatcattg accaaaaagc gatatcctta aatgaataaa aactatccaa
     6301 taagatgaaa acgattgcaa ataatatgat aaataaagtc gtattatgag tagctaagcc
     6361 aaagaaattg gtaaagattg ctaccaagag tacggaacta acaacgccgc cgataactaa
     6421 ccatggtttg aatttaccgt attttgtacg agtattatcg atgatgccac caatcaaagg
     6481 atcaaaaata atttcaacta agcggatacc tacaactaag cttgtaactg tagcaatcat
     6541 ttttgcattc gttgccttat ctgttcctgc gaacatctgg cttgtcacga ataccataaa
     6601 ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa gcgcctaaag cgtaggacac
     6661 acgttggatc attttgcttt tcataatctg tattcctctc ctgttcaata tataatacta
     6721 agtaaaccgg ttacaacgaa taacaaagat cttttaatga cttaaactta acaagaaatc
     6781 gcttcctttt taatatattt ctgtgacttt tatttaactt tttgttgcat tatactaaag
     6841 gagtaaggag ggatagacgt ggagcaatcg atttttttaa aaaaggaaca tcaacaagta
     6901 gaaaaagaat tgtattttga tttttgtggt ttctctaaaa cgatgccgtt tcattctttt
     6961 ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga tggaaggcaa aggatcttat
     7021 catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc tgttcttgat tcgtccagga
     7081 gactcgacgt tttacttatc agatggagaa gatccgtgga tgtattgctg gctttcattt
     7141 ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt tcagagaaga ccaatacacg
     7201 atggtctctt ctgacataca aaaatatatc gacattattt tagaatgcat gaaagatacg
     7261 gaagaaacct tggaaaatga attacagtta acagcattaa cctaccgttt tttatcagtc
     7321 cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa aggttcattc ctctttagct
     7381 gttgaaacag tcaaatacat cgaggcacat tatgctgaaa aattgtctgt tgaggaaatc
     7441 gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag tattcaaaaa tcatatgggt
     7501 accagtatca aggaatattt gatcggggta cggatcaatc gaggggcgtt tcttctttct
     7561 ctgacggatg aatccgtgga aagtattgcc tatcaagtag ggttcaatag tctagtcgta
     7621 tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta cgaactatcg taaacgtatg
     7681 aaaaacgaaa attttaagaa tattgctttg gataccttga aaaaacagtt ggaaaaacaa
     7741 accgcagttt cttggacaac ctgattagtc tggttaaaat gacaagcatc ggcaaaagaa
     7801 acttgtggcg gttatgctac actgtaaagc gaagactaaa aaggaggaaa agggatgcgt
     7861 acacttatca tcattagcca tccaacgatc gaagaatctt taaatcagca atttttcaaa
     7921 gaagcttctg ctggtttacc agctacttgg catcatttgg aatcaagtta tccagacgga
     7981 aaaatagata tagagactga actacagctc ttggcccagc atgatcggat cattttccag
     8041 tttccgtttt actggtatag ttctcctgct catttaaaaa tttggcaaga taatgtcttg
     8101 gaacgagcag cacatgtatt aaaagggaaa gaactaggaa tcgtattgac aacaggtgtg
     8161 gcggaaaaag aataccaagc tggaggaaaa gaagaatata cgatttccga atttttgagg
     8221 ccgtatcagc gaatcgcaaa taaattccat atgacctatc ttccgccatt cgttttagca
     8281 caatttatgt atttatccca ggaaaaacgt tgggagaagc taattgctta tcagcagtat
     8341 ctctcattgg aaggcaaacc atcgctgact cagcggattg actggtttat tcaaagagta
     8401 caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac aagcatatat cgtagaagca
     8461 ctgactgagg caaaagagca gatcgaagac ttgaccttta cactgcaaga aatgaaagga
     8521 acttcattat gacaaatgat tggctgatcc aacaattaga tgaattaagc gaaaaatcag
     8581 aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact gatggctgag atagacgaac
     8641 gaacaatgca gctgcaagga gaaatcgacg gacaatcttg gaatcatctc aactggtgaa
     8701 attttcttat aaataacaaa gataaaaatg tgcacagcga caattatttg ctacaataag
     8761 aatatgaagc gtttgataac gctattatac ataaaaattg gtggtgaaaa aaatgtatga
     8821 tgaaaatgag tggggagaaa atgttcggat gacccgaaga agacagcaaa aacaggcagc
     8881 tcgtaagcgg atgatcatga ttggggcagc agttgccgtg tttttgatca tagcaggagg
     8941 agttgcgggt ctatattttc atggaaaaaa ttcgtcagaa gcgaaaagta cgaccataac
     9001 ggaaacaacg acaaagaaaa cagagacgaa acaaacaagt gagtcatcaa ctaaaacaaa
     9061 agagacacaa acttctgaag aaaaaacagc aaatggtttg ccagagcaac ttcaagcaat
     9121 cgtaaatgat tattcgaaaa agatcgaaga aaagacgcct gcattgattg aagaatacca
     9181 gacagaaatt cagggaaatc aagagggaat agctggttta tcagcagttg ctaaccaaaa
     9241 agcacgtgag cttcaagcaa tctccgacga agggatcaga aaattacgtg cagcgtatca
     9301 ggcagcagaa aataaagacg gtgtagactt agatactttg atcaatcaat tatcggctaa
     9361 ttatacgaat catgtcgcta agatttccga tatttatttg cagactagcg cctctcttca
     9421 agcagagtca acaagcacac aagacacaac atcttctgat tctgagacag cagagtcggt
     9481 agaagcttct gaaatgcaag ctcgaacaac acaagacaca acagattcct ctgaaactaa
     9541 taccaacagc gcgacttcac aggctgcgac aaccgtagtt ttggaaggag aaggtccaaa
     9601 tcaaatagcc gaacgaactg gcgtaccagt agaaacgatc ttgtcgctga atgggatgag
     9661 catggagaat tatttcctga ctcccggtga gacgctaaaa ttgaattgat aaaagcaata
     9721 aaaaagaacg aagcttccac taaggtgaag cttcgttctt tttttatttt ccggttcttt
     9781 tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa aacgagtcca ataatcaatg
     9841 agattctcgt ttcaggattg ataaacatga aaatcaatac gatagctaaa aaagcaagtg
     9901 tcacataatt cgtataagga gatagtggca ttttaaaagg atgatcatgc attttatctg
     9961 cgtgcttctt tttgaagcga atctcgctga tcaaaatcac tacccaagga accataccag
    10021 gcaaaatact tgagctgtat acgaaaacga aaatattttc tgactggttg atgaacattg
    10081 gtagaagata atttaagatc agaccaatta agattccacc tgagattgct acgacaggat
    10141 aaaaaggcgc accgtgacga gatagtttca gaaatttttt agacatcttt ccatttatgg
    10201 caagtgtata gaccatacga cttgcgctga aaatccctga attgcaacct gataatgccg
    10261 ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt aatgccgact ttagaaaaag
    10321 tttgtacaaa aggtgaacca atctcactca attgattcca cggatagata ctcacaatga
    10381 taaaaatagc accaatataa aaaatcaaga ttcgaccgat agtggactgg acagcttcaa
    10441 tgatcgtctt tttaggattc tctgcttcac cagcagtcat tccgatcagt tcgattcctt
    10501 gataggaagc aacaacgatc gataatgcaa agaaaaatcc tttgacgcct ccagtgaaga
    10561 atccaccatt tttccaaaga tttgaaatcc ctactgcctg accatgatta ccaaatccaa
    10621 ataagattac cccaagacct gcaatgatca tcaaaatgat cgttatgact ttgatgatcg
    10681 aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat aagatttgcc agcataagaa
    10741 acaagacgac aatgattccg ggaataattg ccggcaaatc aggccaccaa taattcatat
    10801 aagatcctac agcaatcact tcacttattc ctacgacaat atattgaaaa atattactcc
    10861 aagcggtcaa gtagccagcc acaggatgga tgtactcgct cgcgtaattt gcaaaagaac
    10921 cagtcgaagg atcaacgtaa agcatttcgc ctaaagcacg cataatcaaa tacaaaaaga
    10981 atcctgcaat cgcataggct cccatcactg agggccctgt ccaacgtatg gttgaggagg
    11041 cacccataaa cagaccgaca ccgatcgttc cacctagtgc gatcatttgg atatgacggg
    11101 cactaagact tcgagttaac ttttcttctt ccatacaaaa actcctttcg ttgttgagtc
    11161 aacaagaaaa aataatcaaa cgtttcaaca catgtttttc attattaggt tagccttatt
    11221 gaactcttga tgtgattcta cagaaaattt tcataaaatg cgaggaaaat tcgaaaaaaa
    11281 ctttttcatc attctacttg aaaatgcttt ccattttagt taaactatat ttaaataaaa
    11341 agttaggtaa acctaaaaag gagtgtaaga tttggaacgt acggccattg taattcaaga
    11401 cctttcagcc gcttatcaag ggaaaactgt tttgaaaact atcaacttga cgataaatcc
    11461 acaaaaaatt accggtatta tcggaccaaa cggtgcagga aaatcaacat ttatgaaatc
    11521 tttgctagag ttgataccag ctactactgg taaagtcacc ttttctggac agtcggtaaa
    11581 ctcagtccgt aaaaaaatag cctacgtgga acagcgaagt gaattggatc tttcctttcc
    11641 aattgatgta ttaggcgttg tacttttagg aacatatcca tctttacgaa ttggacaaag
    11701 acctgggaaa aaagaaaaag aacgtgcaag acaagctttg aaaaaagtag ggatggaaga
    11761 atatgccaaa agacagatca gcgaactatc gggtggacag cttcagagag tttttattgc
    11821 aagagctcta gcccaaggag cagaatggat ctttttagat gaaccattcg tagggattga
    11881 tgcgttaagt gaacgaaaga tctttgacat cttgcaggaa ttgaagaatt caggaaaaac
    11941 gattttgatc gtccatcatg atcttcataa agtagaagaa tatttcgatg aggttattct
    12001 tttaaataaa cagctgatcg cttccggtcc agtacaagaa gtctttacat cgaagaacct
    12061 tcaattggcc tatggtgaga tcatcagaca tttagtaaaa ggaggagaga aaaaatgatc
    12121 caatccttta ttgatggatt aatgaattat caattcctgc aatacgcttt ggtcacttcc
    12181 atgcttgtag gacttgcttc tggagtcatc ggttctttta ttattttacg agggatgtct
    12241 ttgatgggag acgctatctc tcacgcagtt cttccaggtg tagccatttc ttatatgttt
    12301 ggttttagtt acatctttgg agccactgct tttgggatgc taacagcagc agtgattggt
    12361 ttcgtgactc agcggagccg attgaaaaat gatacagcga ttggaattgt ctttagctcc
    12421 ttttttgcat tagggattat cttgatttca tatgcccaga gtgcgacaga tttgtatcat
    12481 attttatttg ggaatgtctt agctgttagg gaaagcgatt tgttgctgac agcactcgtt
    12541 agtggaatcg tcttgatatt tgtctttttc ttttataaag aattgaagat cacatcattc
    12601 gatccgacaa tggcaaaagc ttatgggctg aacacatcgc tgatccatta tcttttgatg
    12661 ttctttttga cattagtggc tgtagtcagt ttacagacag taggaacaat cttggtgatt
    12721 gccatgttga tcacaccagc cgccacggct tacttgctaa cgaaccattt actgaaaatg
    12781 atcattacag ctgcaggaat cggtatgcta agtgcagttg tcggtgtgtt tttcagttat
    12841 agttacaatt ggccatcagg agctacgatc gtgttagcat gtaccgcatt ttttatcctt
    12901 gcttttttat tttctccaac aaaaggaatt ttatttcaga aaggaagcag atagatgaaa
    12961 acaaaaaaat cactcttttt aatcttggca gttagtttct tggtattagc cggttgcgga
    13021 aagcaggctt ctgaccaagc ggatgaagga agtaaagaaa agttgtcagt cgtggctacc
    13081 aattcgatct tggcggacat ggcaaaagag gtcggaacag atcaaataga tatccacagt
    13141 atcgtaccag tcggaacaga tccgcatgaa tatgaagtac taccagaaga catcaaaaag
    13201 gcaagcgatg cagatgttat tttatacaac ggtttgaatc ttgaaacagg taacagctgg
    13261 ttcgataact tgatggaaac ggctaaaaaa gaagaaggaa aagattattt tgcagttagc
    13321 aaaaatgtag aacctcttta tttaactagc ggtgaagaac atacaaaagc agatccccat
    13381 gcttggctag acctatctaa cggaataaaa tatgtggagg aaatcgcacg tatattctct
    13441 gaaaaagatg cagaaaatgc gacactctat aaaaaaaatg cagaagcata tgtggaaaaa
    13501 ctaaaagaat tagatacaca agcgaaggaa agttttgctt ctatcgaaga gaacaaaaaa
    13561 ttattagtaa caagtgaagg ggcattcaag tatttttcac gagcatatga tttgccagca
    13621 gcttatatat gggagatcaa tacagaaagt caaggtacgc ctgatcaaat gaaagcgatc
    13681 attgatcaga taagagcaaa agaagtaccg gttttattcg tggaaaccag tgtagactca
    13741 agaagcatgg aacgggtagc aaaagaaacg ggtttgaaaa tctatgataa actgttcact
    13801 gattccatag caaaagaagg agaacaagga gattcttact atcagatgat gaaatggaat
    13861 attgaaacga tccatgaagg tttgtcacaa acaaaagaaa gctgaaaagt gcaaggtcta
    13921 gcttgaggag cagaccttgc attttttata agagatatga aaaaaaatta tgctgttttt
    13981 cgtcgtacag ataccttttt tgaagaaatt ccgttatagt tagagtaagg aaggaaggtg
    14041 tggctatgaa ttttacacgt aaagataaat atacgattaa agaagaagtc tttcgattga
    14101 tcagccagca ggaaaatacc gacaaggatc aagaccttca atttcttgaa gcagaattag
    14161 ctaaattatt catagcaaat caaaatgaga caacagaacc agatgaagag ctgtacatag
    14221 gcaaatggtt gtttgatgaa tttcagcctt tagatgaaaa taccggtatc ggttattttg
    14281 tgttcctttc tccagtcggc ttcacacctg gcgtattagt taaacaaaac gacgaaataa
    14341 ccgtcaaagc atggcctgca ttattttcag tcatcaaagc agaaggagta atcaaaaaga
    14401 tccagtcggc ctattataaa agtcaattta ttgccaacag caaattaatg aatatgtttt
    14461 ataatcgtct agtagaaatt gctaggcctg ggaaaatccc gcaaacattc acgtacacac
    14521 agcaaaaaaa tctctatgaa gaagtattgt attcccgcga tctgtctgag ttttcgccat
    14581 gggaagaggt aaggctccat gaactgttaa gtacaaaacc agtttcattg aattttttta
    14641 atgcattaga taacaaaaga aaattaattt caacaaatta ttgggaagta tttgctgcaa
    14701 gaaaaaaatg gatggaagaa aagaagattg ccgatcagca gatccagttt gtccagctga
    14761 aaaaagaaaa actatattat gggttataca gccgttaaca gcaagcgagg aagaccatga
    14821 cattaaaaga agaaatcata caagaaagca aacgattagg aattgacaaa atcgggttca
    14881 ccacggcgga accttttgat tcattaaaag aatcgttgga agaacaaaaa gcagctggcc
    14941 atacttctgg ctttgaacat ccaaatatcg atgagcgtct ttatccagaa atgacatttg
    15001 atcagccgaa atcgatcatt tccattgcac ttgcgtatcc tactaggatc catgaagaag
    15061 tgcctaggga tgaaaaacgc gggcagtttg ctcgtgcttc ttgggggatc gattaccatg
    15121 atatactcag agaccgtttg aaccgattga tcgaattcat ccaaacaaga gcagaaaaat
    15181 ggcaaaaaga agaggaatgg cgtctagctc cgcaagtaga tactggagaa ctgatcgatg
    15241 tcgctgtagc acagcgagct ggtttaggat ttattggacg aaacggattg ttgatcactg
    15301 aagagttcgg ttcatttgtc tatcttggtg agatcgttac taatattgta tttgagcctg
    15361 ataaaccagg agaatttggt tgcggtgact gtatgcgctg cgtgactgct tgtccgacaa
    15421 aagcattgct tggtgacgga agaatgaatg cccagcgatg tttgtcttat caaactcaga
    15481 caaaagggat gatgccggaa gaatatcgta aaaaaatgcg aaatgtcatt tatggatgtg
    15541 atatctgcca gttagtctgc ccttacaatc aaggaaaaga ttttcatttt catgaagaga
    15601 tggaaccaaa agtagacgaa atatatccaa aattaaagcc aatgctttcg atgtctaata
    15661 aagacttcaa acagcaattt gggcatttag ctggttcatg gcgtgggaag aaacctttgc
    15721 agagaaacgc attgattgct ttagctaatc taggtgatcg aacggcattg cctgagattt
    15781 cagaatgttt aacagatgtc cgccctgtca tcagaggaac agcagcttgg gctattggac
    15841 gtctaggaac aaaagaaccg gaaaaatggc ttgatgtatt agatgaaata ttagctaaag
    15901 aaacagaaga agtcgttata aacgaattgg aacatgccca gaaattattg cggagaaagt
    15961 taaaataaaa aaatagttaa cattattcaa agtttttgtc cagtaattgt tgtaacaatt
    16021 actggttttt ttgtattttt tacatttttt taagagaatt tgat
//
LOCUS       DAHHZJ010000051        15922 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.052, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000051 DAHHZJ010000000
VERSION     DAHHZJ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.052"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(59..187)
                     /locus_tag="NBL02_001315"
     CDS             complement(59..187)
                     /locus_tag="NBL02_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCD2572009.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="NBL02_001316"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="NBL02_001316"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCD2572010.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="NBL02_001317"
     CDS             complement(1674..2729)
                     /locus_tag="NBL02_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCD2572011.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="NBL02_001318"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="NBL02_001318"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HCD2572012.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="NBL02_001319"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="NBL02_001319"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HCD2572013.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="NBL02_001320"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="NBL02_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HCD2572014.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="NBL02_001321"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="NBL02_001321"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCD2572015.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="NBL02_001322"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="NBL02_001322"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HCD2572016.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="NBL02_001323"
     CDS             10491..12050
                     /locus_tag="NBL02_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HCD2572017.1"
                     /translation="MKKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="NBL02_001324"
     CDS             complement(12161..14092)
                     /locus_tag="NBL02_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCD2572018.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="NBL02_001325"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="NBL02_001325"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCD2572019.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="NBL02_001326"
     CDS             complement(15021..15773)
                     /locus_tag="NBL02_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCD2572020.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
BASE COUNT         4114 a         3486 c         2906 g         5416 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcccgc ttttttgaag taagcgtcat aataaccagc acttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tctgagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 agttggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcttcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgcttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttctgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgacagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcaattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg tgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ttccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggga attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat atcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt tgccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccgatcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatatgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atgaaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaaggtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtggtg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagagac tgggaaaaac cagcctcttc ttttttattt ctatgcttct gctgctgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgcca tgaggagcca
    12301 gtaatttgat cccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcagctc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcagagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccggcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagcaat tttttaccat acggaatgat ttcttctaca gattggaagg ttgtattgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaat ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagccggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cccaaaccgt taatttcagt ttcagtatct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg ggtccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattaaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctacgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctactc gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcaa aaagactggt
    15901 tttttatttt tttgtctatc at
//
LOCUS       DAHHZJ010000052        15468 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.053, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000052 DAHHZJ010000000
VERSION     DAHHZJ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.053"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(89..808)
                     /locus_tag="NBL02_001327"
     CDS             complement(89..808)
                     /locus_tag="NBL02_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCD2572021.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(939..2090)
                     /locus_tag="NBL02_001328"
     CDS             complement(939..2090)
                     /locus_tag="NBL02_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HCD2572022.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(2307..3047)
                     /locus_tag="NBL02_001329"
     CDS             complement(2307..3047)
                     /locus_tag="NBL02_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCD2572023.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(3051..3374)
                     /gene="rsfS"
                     /locus_tag="NBL02_001330"
     CDS             complement(3051..3374)
                     /gene="rsfS"
                     /locus_tag="NBL02_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCD2572024.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(3431..4024)
                     /gene="yqeK"
                     /locus_tag="NBL02_001331"
     CDS             complement(3431..4024)
                     /gene="yqeK"
                     /locus_tag="NBL02_001331"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HCD2572025.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(4008..4658)
                     /locus_tag="NBL02_001332"
     CDS             complement(4008..4658)
                     /locus_tag="NBL02_001332"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCD2572026.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(4668..4979)
                     /gene="yhbY"
                     /locus_tag="NBL02_001333"
     CDS             complement(4668..4979)
                     /gene="yhbY"
                     /locus_tag="NBL02_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCD2572027.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(4995..6104)
                     /gene="yqeH"
                     /locus_tag="NBL02_001334"
     CDS             complement(4995..6104)
                     /gene="yqeH"
                     /locus_tag="NBL02_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HCD2572028.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(6101..6628)
                     /locus_tag="NBL02_001335"
     CDS             complement(6101..6628)
                     /locus_tag="NBL02_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HCD2572029.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(6808..7593)
                     /locus_tag="NBL02_001336"
     CDS             complement(6808..7593)
                     /locus_tag="NBL02_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCD2572030.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(7586..8455)
                     /locus_tag="NBL02_001337"
     CDS             complement(7586..8455)
                     /locus_tag="NBL02_001337"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HCD2572031.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(8456..9835)
                     /locus_tag="NBL02_001338"
     CDS             complement(8456..9835)
                     /locus_tag="NBL02_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCD2572032.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(9843..10262)
                     /gene="fabZ"
                     /locus_tag="NBL02_001339"
     CDS             complement(9843..10262)
                     /gene="fabZ"
                     /locus_tag="NBL02_001339"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCD2572033.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(10287..10763)
                     /gene="accB"
                     /locus_tag="NBL02_001340"
     CDS             complement(10287..10763)
                     /gene="accB"
                     /locus_tag="NBL02_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCD2572034.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(10766..12001)
                     /gene="fabF"
                     /locus_tag="NBL02_001341"
     CDS             complement(10766..12001)
                     /gene="fabF"
                     /locus_tag="NBL02_001341"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCD2572035.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(12020..12757)
                     /gene="fabG"
                     /locus_tag="NBL02_001342"
     CDS             complement(12020..12757)
                     /gene="fabG"
                     /locus_tag="NBL02_001342"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HCD2572036.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(12769..13686)
                     /gene="fabD"
                     /locus_tag="NBL02_001343"
     CDS             complement(12769..13686)
                     /gene="fabD"
                     /locus_tag="NBL02_001343"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCD2572037.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(13793..14017)
                     /locus_tag="NBL02_001344"
     CDS             complement(13793..14017)
                     /locus_tag="NBL02_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCD2572038.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(14070..15032)
                     /locus_tag="NBL02_001345"
     CDS             complement(14070..15032)
                     /locus_tag="NBL02_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HCD2572039.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(15042..>15468)
                     /locus_tag="NBL02_001346"
     CDS             complement(15042..>15468)
                     /locus_tag="NBL02_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCD2572040.1"
                     /translation="KTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIEAIGMY
                     KKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGKLLFRV
                     HQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         4052 a         3534 c         2664 g         5218 t
ORIGIN      
        1 taaatcacac aaaaaagcaa gacaatctgt tagtgagatt gtcttgcttt tcgaaaaaga
       61 ctgaatactt ttcgttaaaa ctggttgctt agtcttctaa tgccacatta tggaacacat
      121 gctgtacatc ttctagatct tccagtgcat caatcatttt ttcaaatgtt tcttgatctt
      181 ctggagatag tgttacctcg ttttgaggga tcatttggat ctcagcgatt gaaaaatcag
      241 tgattccttt ttcttttaat gcttcttgta tactatggaa atccgctact tcaccgtaaa
      301 cgatgatttg tccttcttct tccattacat cgcgaacatc gatgtctttt tccattaggt
      361 attcaaggat ctcgtcagcg tcttctcctg caaaagcaaa gattgctgta ttgtcaaaca
      421 tgtaagaaac cgctccggaa acacccatgt tcccgccatt tttcccaaat gctgcccgta
      481 cgtctgctgc tgttcgattc acattgttcg tcaatgtatc aacaatcacc attgagccgc
      541 ttggtccaaa tccttcatag cgcaattcag agtatgtttc atctcctgca ccttttgctt
      601 tttcgattgc tcggtcaata atatgttttg gtacattata agttttagcg cgatcaatga
      661 cgaaacgtag tttttggttt gcatgcggat caggatcacc tgattttgct gccgcgtaga
      721 tttcaatccc aaattttgca taaatccggc tattattagc atctttagca gttttctttg
      781 ctacgatatt tgcccattta cgtcccatat tttcacttcg ctttctgatt tgatagtttc
      841 ataacgcaaa aacgtcctgt tccattatac ttgtgaatca cgattctgtg aagctttttt
      901 tctttcttcc ttcattcttc tcatttttag tcaattatct atactcggat tgggatttgc
      961 ccgaaattct gttccggaat attgcctccc aattgataga tttgatcact tctaactaac
     1021 agttccgctt cttgtgcctc tttttttccg attcgggaat aaatagaaat ctcttttttt
     1081 tcttttaaga atcttcttcc agcatcggta aatccaagta gtcgaagata atttttattt
     1141 tgttggacag gaatctcttg ctttttgata ttcagcaaga cataacaaga taatcgttgg
     1201 atccgtgtcc atgtatatct ttttgttttt acctttgaca caaaatcctc aaaactatca
     1261 gcagttgcag ctgctgtttt catgcgcgat tctattcctt cggtcatctg atagatatct
     1321 gtcagttcca gcaaagtaga agataacaac cgataacgca aatacggcca ataagattcc
     1381 caagtctgca gcgaacaagt tgaaagatct tcttctgtct gaggcggcac aagtccagct
     1441 attctttttc cttcttctaa cgcatggcga atggctgttg cactggctat cctctgatct
     1501 gtgatgattt tttcatgata gcctgcactt ttacgtcgga tcggatgtaa ttccataggg
     1561 tggacatact tggcattttc ctttgcataa cttaatccca agatatgatt tggcgaagaa
     1621 aaatcaagcg taatatcggg atacagactg cgaaaaacct cagtcatttt ttgcggataa
     1681 ctatacgttt gatcagacag ttcttgaaat gcctcttcta tcctatcttg attctcttgg
     1741 acaaagcgtc cgaaactgct gtaatcaaac gaagtctctt cttctgttcc aaaacacaaa
     1801 ctgtcacagt ccagtgtttg caagattttt actgcacctt tagcaaaata gtcagctgac
     1861 tgaaccgccc attcgacggg aagctccact actaaatcca caccattttg taaagcttca
     1921 ttcgcgcgat gccatttatc gatgatagcc ggttcgcctc tttgcaaaaa attcccgctc
     1981 attacagcaa tgacgacctc tgctcctgtt atttttcgtg ccatttccac atgatagcga
     2041 tgtccattgt gaaaaggatt gtactctacg ataatcccgc agcttttcat gcgcatcctc
     2101 cttcatagtt atcgataaaa aagaaataga gacaagtaaa aaaaggcaaa aatcggaaaa
     2161 aatacccgaa ctatataaac tattctcgca aacacaatac ttctgcgacg aaaagctgca
     2221 ctgcaagagc aatgcctata tcgttcggat atataattta tgttgaagat ttattctttt
     2281 cttgttaact cgagcaggca aaaccgctag tctttgtatg ctacaaagaa ccagcgcttt
     2341 gatttctctg atgctgcttc gtctgtaaaa tcagaaaatg cttcgacttt cgaaaagccg
     2401 ctgttttcca gcattcttaa ataactttcc aatggatagg tacgctcttg atgtagttca
     2461 tcttcacgaa taaatcgacc agacccttct tgttcttcaa cgaaaaaggt aagaaaatgt
     2521 tcgatgctgt gtttctcgct tcctggataa ctatcccaca aaaaagcaaa attatcccct
     2581 tgataatgat agctatattc tggaaaaacc tcatctactt gataggtgga gtgtacgtca
     2641 aataaaaatc ggccattttc ttctaacgct tgatagactt catcaaacac ttgctgtacc
     2701 tcttgtctat ttttcatata gcacagcgaa tcagaaaaac aggtaattgc ctgatattgt
     2761 ccgatttctg ataaatcgag catattgcct tccacaaatt ggatcgatgc gtcttcttca
     2821 tatgctcgct ggctggcaat catcaacatc tcttctgaaa ggtctaaccc tgtcacttta
     2881 tatccatctt ttgcaaaggc aaccgctaat gctccagtac cgcatgccag ttccaaaata
     2941 ttttttgtgt cttcttgtaa atgacgtttc gaaaaagcta accattgctg ataaagtgtt
     3001 tcgtccatca cttcatcgta aatgaaagca aatgtttcat aagccatatg ttaatccaac
     3061 cattcactaa gatcgactag cggagcatct gaccatagtt tttccagatt atagaaattt
     3121 cgttctggcg catggaaaac atgtacgatc acatctccaa gatcgatcaa tacccatttt
     3181 ccgccttctt ttccttcaat acgttttact tcatatttgt tttcttcttc tttatcaatg
     3241 atttcttctg taattgcgtt gatttgacgt tcgctatttg ctgaacagat catgaaataa
     3301 tctgccaaca aggaaacatt tcgaacgtct aaagcgataa tgtcttctgc ccgttttgaa
     3361 tctgctgctt caatcgcaat ttttaattgt tctttactat ctatgatgat ttcctcctaa
     3421 tatgagcagc ttattttttt gctacccagt aattatatgt ttcgattgtt tttggataaa
     3481 tctgttgttc ttgcgcaagc aaatgggcca atgtatgttt tgtttcaaat gctacagctt
     3541 cgtctagatc agcccatgca atggctcgtg catcttgtac cccaggaaaa tctcgacctg
     3601 gttcaatata gtctgctaca taaatgattt tgtcaaggag actcatctta gcagccccag
     3661 tcgtatgcac ccggattgca tgaagaatct ctgcatcggt gattcctaat tcccgctcta
     3721 caaacgaagc gccaacaaga ccatgccaga ttgcattatt ataattcaat aactccggat
     3781 caaaaccgtc tcttacgatt accattttga actcctcatc tggtcgttct tttgcataat
     3841 catgtgtcaa tgcagcaata ctagcttttt caggagaagc accgtatttt ttggcaagag
     3901 ccacggctgt ttcttctact ccaagcacat gcttgaagcg ttgttcgctc attctcatct
     3961 gtattttttg catcaggttt tctctagaat aagcagtata ttcattacta tagtccatat
     4021 tcatacaacc cttcattttg aatataatca attactttat ctggaacaag gtagcggata
     4081 gaacagcctt ctttgatctt ttgacgtatt ttcgtggaac tgatgtcgat ctcaggtaca
     4141 tctacccaaa ttacaggata tggagtatcc gttgtgtatc ctggtcgacg tatacccaca
     4201 aagttgacca tagaagtcag ctcatcgatt ttataccatt ttggcaaata ttctaccata
     4261 tccccaccga taataaagta ataatctgta tccggattgt tttgcgtcaa ttccttcata
     4321 gtatcatagg tgtaactttt cccaccacga gctaattcaa tcgtttcaat ctgcaaaaaa
     4381 ggattgtctt ccaccgctaa ttccaacatg ttgagtcgat gttttgcatc aatcgtctgt
     4441 ttctcatcaa catgaggtgg ctgataagaa ggcatcaaaa atacgcggtc aagacctaaa
     4501 ttccggccag cttgttcagc catcactaaa tgtgcaaggt gaacgggatt gaatgttccg
     4561 cccaagatcc ctacttgctt tttcttctct aagaattgag gcatttcttc aggaaataat
     4621 tgtgaacgaa caaatgtctt agcttgcgta ttcatcatgg tcactcctta tattgctttt
     4681 acttcttttg atagcctttg atacttttct tttgtggaag gtttgtataa tacaataaca
     4741 cgtccaatga tttggacgat ttcgcaatct atttcttttt ctaatacttg agctgcttct
     4801 tctgcaatct catctgtatt ttgaagtaac gatactttga tcagttctct tttttctaaa
     4861 gcttcttcaa tttgaacgat cattgctgaa ttgatcccac cttttccgat ttgaaaaatc
     4921 ggctgaagat gatgcgcttt gctacgtaaa aaacgctttt gttttcctct taaactcata
     4981 attttccttc ttccttaaat caatgctttg cgtttcaata catctacacc ctttggcgcc
     5041 cagcccgcaa ttactactgg cgctgttaca gtgatccagc ctagaccggc aaaaacgatg
     5101 tctgtttttt ctttcacaga aaactcaaaa cgaacaagct ccggaaatgc ctcgacttca
     5161 tcaggacgag gaggctgtaa taatccacct acatgttttt gataaaattc gtctgctttt
     5221 tccagtttcg ttcgatgaat atttacatca tttgacacat aagcaataaa tgaagcacga
     5281 ttgccgctga taaaatcaaa tcttgcaagt ccgcccaaga aaagtgtttg cccttcattc
     5341 agctgataaa ctttaggttt gatttctttt aacggtgcaa tgatccgcaa atctttttta
     5401 cctaagtagt gcgccatttg atggcgatgg ataattcccg gtgtatcaat caaaaaatgc
     5461 ccgtcatcta aaggaatctc gattttatcc aaggtcgttc ctggaaactg cgaagttgtg
     5521 attacatctt gtactcccgc tgtatttttg atgatttgat tgataagcgt cgattttccg
     5581 acatttgtta ctccgacgat atagacatcc cgtccatcgc ggtattcttc gattttttct
     5641 aatagttccg tcatctcatg agatttctta gcactagtta ataatacatc tactggacga
     5701 attcctgctt cgtaagcacg ttccttcatc cattgtgtta atttagtttt tttcaatgat
     5761 ttaggtaata tatccacttt gttcccaact aaaagtactg gattgtcacc gacaaaacgg
     5821 tgtagtcctg ggattagtga accattgaag tcaaaaatat ccaccacatt gacgatcaat
     5881 gcatcttttt gccctaattc attcagtaag cgcaaaaagt cgtcatccgt caatgagaca
     5941 tcttggatct cattataatg gcgtaatctg aaacaacgct gacaatagac ttccccggaa
     6001 gctaaacctt tttccaaagc cgtttgcggc gtgtatccta attcaccggg tttgtttgtt
     6061 tgtataacgg caccacagcc gatacattgt aattcttctg tcattcgatt ccacctcgcc
     6121 agcccatatc agggtgtttt gctaatagat aggccataat tcttttttcc caaaaacgat
     6181 taaattgtgt cttccaacta tctgttgcga taatgggttt tacaaggatg ctacgtatcc
     6241 ctgcacgatt ggctccacgt atatcagtca taatctgatc gcctaccatt actatctcat
     6301 catctgctaa atccaattgt ttctttgcta tgttgatccc tcttgctaaa ggtttcattg
     6361 ctcgtgcaac atacaaaaga tcaaattttt caatcgctcg cgcgacacgt tttgaattat
     6421 tgtttgaaac aacaacaaca gggattcctg catttttcat ttctaaaatc caagtaagca
     6481 actcttctgt tccatctgga ttattccatg caatcaacgt attgtctaaa tcggtcaata
     6541 ctgctttgat gcctagattc tttaattggg caggtgtaat ctgatagatg gcttctacca
     6601 tccatgttgg tttgtaattt gaaaacatgc tgtctcctca tctgcaaaaa atgaaggagc
     6661 acataaagta ctcctcaacc ccgtattata ctgcatatca gcaaaaaaaa ctacctctat
     6721 actcgttcgt ctttcagaaa aagcatttct ttacaaaatg accaaaaata aagattgatc
     6781 acagaaaaat ttagaaacaa aaaacaacta atattggcgg aacctcttgt atcgtttttc
     6841 cagtaattta tgctgatcta atcgaacaag ttccgtcaat ctttcaatca acgctttttt
     6901 cataatgtgt gcgactttat atcgtggcaa ctgctcgccg ttgaacgttt caggaataac
     6961 tcgatcaacg attcctaact cgcgtagttc tggcgcagta attttcatca actcagctgc
     7021 ttctgcggcg cgactaccgt ctttccacaa aatcgacgca aatccttctg gagaaagcac
     7081 agcataaatc gtttcttcca gcatccagac ttcatccgct aaacctagag caagggcacc
     7141 gccacttcca ccttcaccaa tgatgatggc gatgatcggt acctttaaat cagacatttc
     7201 gagcagattt ctggcaatag cctctccttc gccgcgctct tctgctccta caccgcaata
     7261 tgcacctgct gtattgatca aggtaatcac cggtctattg aacttttccg cttgtttcat
     7321 cagtcgtaaa gcttttcgat agccttcagg atggggagaa ccaaaatttc tgcggatatt
     7381 ttcctgcaga tcccgtcctt tttgaatacc gatcactgtt accggtttgc catctaatcg
     7441 agcgattcca ccaacaatcg cttcgtcatc tgcaaataaa cgatcaccat ggcattcctc
     7501 gaaagaatcg aagatctctt caaaaaaatc caagcttgtc catcgttctt tagctcttgc
     7561 taactgaacg acatcatgtg ctgtcttatc cacgatctat ccatcctttc tgtgtatgca
     7621 attcaagaag cgtatggatt ttctgtttca attccatgcg gggaacaatc tgatcgacaa
     7681 aaccatgaga taataagaat tcagcctttt ggaaatcttc tggaagttcc tgcttgatcg
     7741 tctgttcgat gacgcgtctt ccagcaaaac cgatcaagct ttggggttct gctagaataa
     7801 tatcgccttc cattgcaaaa ctggcagtta ctcctccggt cgtcgggtcc gttaaaacag
     7861 ttagataaaa aagaccttca ttgctatgcc gtttgacagc agcagagatt tttgccatct
     7921 gcattagaga aaagattcct tcttgcatcc gtgcgccacc tgaagctgtg aacaagacga
     7981 ctggaagact ttcctttgtt gctcgttcga ataatctggt gatcttttct ccgacaatcg
     8041 ttcccatact tcccatgata aaattaggat ccatcactcc aataatgaag ggaatcccct
     8101 gaatggttgc tttaccagtg aggactgctt catgaagtcc tgttttatct tgcatttttt
     8161 taattttttc cgtatattcg ggaaatgcta gcggatcttt ggttacaaga tccgtatccc
     8221 attcttcaaa acttttttca tcaataatca aagctagacg ctgccatgca ccgattcgaa
     8281 aagcgtatcc gcaatgaggg catacttttt cttcaccgat gtctttcgta taaaggatat
     8341 gtttgcaatt tggacacttt gcccacatgt tatcaggaac agatggcgag ttagcaggtt
     8401 tgctctctct attaggattg atccgaatat atttcttttt cttaaacaga gccatctatg
     8461 cgcctcctat ctccgtttct ggtgtccaat tcggtaaaaa ttcttcttgt aaaaatgctg
     8521 tgctgtaatc acctgctatg actgcaggat gactaatcaa atccatctga aattctgcgt
     8581 tcgtgatgat tccttctgtg ataaattcac ttaaggctcg ctgcatcttc attaatgctt
     8641 caaaacgcgt atttccatgg acgattattt ttgcaatcat tgaatcataa tatggcggga
     8701 tcgtatatcc cgggtaaact gcactgtcta ctcgcagtcc catcccacct gctggaagca
     8761 gtaagttttg gatcttccct ggagaaggag caaaatggaa agcaggattt tctgcattaa
     8821 tccggcattc gattgcgtgt ccttggaata ctacttcttc ttgacacaca ttcaaaggct
     8881 caccggctgc tatctccacc tgttttttta ctaaatcgat tcctgtgacc atttctgtta
     8941 ctggatgttc cacttggatt cgagtattca tttccatgaa ataaaaatcg ccttcttcat
     9001 ccatcagaaa ctcgatcgtt ccggcatttt cataattgac tgctttagct gctcgaacag
     9061 cagcgtctcc tagtgcttct ctttttgttt gtgagatcac tacagaagga gattcttcca
     9121 gtactttttg attgttgcgt tgtaaagaac aatctcgctc gcctaaatga atgacatgac
     9181 caaatcgatc acctaagatc tgaacttcta tatggcgagc aggataaatg attttttcca
     9241 aatacatatc atcatttcca aatgccgctg ctgcttcttg ctgagctgaa gaaaaatgct
     9301 gtgggagctc ttcttttgat tgaactttcc gaatgccttt cccgccacca cctgcagctg
     9361 ctttcagcat cactggataa ccaattctgt ctgctatttc caatgcttct tctactgtag
     9421 ataatacacc agtacttcct ggaatgactg ggacatttgc ttcttgcatc aattgacgcg
     9481 cattgatctt atttcccatt gcatcgatcg ttgcactttt aggaccaata aatgtgatat
     9541 tgcactcttc acacatggag gcaaatcggc tattttcaga taaaaaacca aatcctggat
     9601 gaattgcttc ggcttttgtc acgattgcag cgctcagtac ttcctgaaca ttcagatatg
     9661 aatcaacggc ttttgccggg ccaatgcaga ttgcttcatc tgctaactgt gtatgaagtg
     9721 cttcggcatc tgcttctgaa tatatcgcta cggtttggat ccctagttcg cggcatgcac
     9781 gaatgatacg tacagcgatt tcaccgcggt tagctatcaa tactttagaa aacatatttc
     9841 acctatccaa tcataaaggt taattccgct tcggctactt ttttaccgtc tactttagcg
     9901 attcctttgc ctattccggc tgaagctttc actttcaata tctcaacttc aaggtataag
     9961 gtatctcccg gtgtaacttt cttacggaat tttgctttgt ccaatccgcc gaaataagcc
    10021 gtctttccct tgaattgttc aagagaaagt aatgcaactg ctcctgcttg agccattgct
    10081 tcaacaatca atacccctgg cataacaggt tcttctggaa aatgtccttg aaaaaagggc
    10141 tcattgatcg ttacattttt cttagcaacg atccgttctc cttcaaccat ttcttccact
    10201 cgatcaatca aaagcattgg ataacgatgt ggaatgattt cttttatttc ttgtatgttc
    10261 attgttttcc ctctttcctg ctttgcttat ttttgctgaa tcctaaagag tggctgtcca
    10321 tattcgacaa ctgactcgtt ttctacaagg atttctgtga tcactccgtc ggcctcacta
    10381 gttatctcat tcatcagttt catcgcttca ataatgcaga taacttctcc tttggcaact
    10441 tgatcaccta cttttttgaa agctggtttg tcgggcgctg gttgcagata aacgattccc
    10501 acgattggcg aaacaacctc gattccatca aatactgccg attctttttc tttcattatt
    10561 tctggagcag ttgttgcttg ttcagatatg acattgactg gctccgaaac tggcaacgtt
    10621 tcgtttgttt gaaccatatt catgcctcga cttgttttat ttttattcat atacaattca
    10681 aattgacctt cacgcaaatc gaattccgtt agcgaggatt gatcaaattg actgactaat
    10741 tctctgattt cattgatatc caaaattatt cctcccagcg tttcatgcac agaaccgcat
    10801 tatgtccgcc aaaccctaat gaattactca ttgcataagt aaaacttgca ggtttgcttt
    10861 cgtttttgac aactgtcaag tcaatctcag gatcttgttt ttctacattg atcgtaggtg
    10921 ggataaattg atgttctaat gccagcaaag ttgccaaggc ttcgatcccg ccggctgctc
    10981 ccaataaatg tccagtcatc gatttcgtgc tgctgacgta agtatctctg aaattttcac
    11041 ctaatgcgat ttggattgct tttgcttctg cctggtcatt ggctggtgta cttgttccgt
    11101 gagcattgat atatccgact tgttccggct gtacaccagc ttcttccatc gcaagcttca
    11161 ttgctttaga tgcaccagaa ccatctggag taggagctgt catatggtaa gcgtcacagt
    11221 ttgcaccgta tccaacgatt tctccataga ttttggcacc gcgagcttgt gcatgttcta
    11281 aagattcaag aactagaacg cctgctcctt cacccatcac aaaaccattc cgattttcgt
    11341 caaacggaat cgaaccacga ttaggatcag ttgattctga gagtgctgtt aaagctgcaa
    11401 atccagcaat ccccatctca caaatcgttg cttctgttcc tccagcaaga atgacatcag
    11461 aatagccatg tttgatgtta cggaatgctt ccccaattga attcgttccg ctagcacaag
    11521 ctgttacgat agctgtacaa ataccttttg cacctacacg caaagcaata tttccagctg
    11581 ccatgttggc aatcgccatt ggtacgaaca ttggagaaac tcgttttgca cctttttcat
    11641 gtagacgaat aacttggttt tcgattgttg gaagaccacc gatcccagat ccgatgatga
    11701 cgccaaaccg gtcttgattc attttttctt tatctaaacc gctcatctcc attgcttcta
    11761 aagcagcgta gattccatag atcgaaaaca tatccattcg tttgctatct tttttaataa
    11821 aatatttatc gtaagggaaa tctttgactt cacccgctaa ggtaatccct gtttctgaag
    11881 catcaaattt agtgatcggt gcgatgccat tcgtcccctc ttttaagcta ttcaaaaagc
    11941 tgtctgcgtc atttccaata ggtgaagtga ctccatagcc agtaattacc actcgattca
    12001 tttgtttgct ctccttttct tatccgtgca tgaccaatcc gccatcgaca ttgatgactt
    12061 gtccggtgat atatggattt tgtgctaaga aaactgctgt ttgtgcaata tcttctactt
    12121 gtccgaaacg ctgcatcgga atttgctttt ccgcttgttc cttgactttg tctgctaaaa
    12181 cttccgtcat atcagttgtg atgaatcctg gcgcaatggc attacatgta atgcctctag
    12241 ttgcagcttc tcgtgccaca gattttgtta atcctactac gccagcttta ctagctgcat
    12301 aatttgcttg accaatattt ccaatcaacc cagatacact agagagatta atgatggccc
    12361 cttctcgctg cttcatcatt tttttcaata cgtgctgcgt catattgaag gttccaatca
    12421 aattgatatc cagacatttt ttgaaatctt ctgcatccat tcgcatcaca agtttgtcat
    12481 ttgtgattcc ggcattgttt actaacacat ggatcgatcc aagcttttct tctgcttctt
    12541 tgatcatttg tcccgctttc tcataatcag agatgtcacc ggaaactcct acgcatttta
    12601 caccgaatgc ttcgatttct tctatctttt cttttggaat ctctccgcga ccattcaaaa
    12661 tgatatttgc tccagctttt gcaaaagcta aggccatcgc tttgccgatt cctcttgtac
    12721 tacctgtgat aaatacattt tttcctttta catccataac gttcctcctt atagtattgc
    12781 tattgtttca tcaaatgtct gcttattctc aacacgcaga actggtattg ttttatcgat
    12841 ttttttcata aatccgctta atacttttcc ggggccgact tcgatgactt gttcgatgcc
    12901 gattgttttt aacttatgga tactctcata gaaacgaaca ggttgcatga cttgctgttc
    12961 aagtagtccc gcgattgttt ctttcttcat tatttccgtt gttgtgttgc ttatgatagg
    13021 aaaagaaggt tcagagaatt gaatctgact taggtccttc gccagttttt ttgctgccgg
    13081 ttctaaaata gctgtatgaa aaggcccgct gacatttaga gggatcattc ttttgaaccc
    13141 tttttctttt aatagtaaaa cagcttcgtc tactgctttt tcttcgccac caatcacgat
    13201 ctgctgaggt gtattataat ttgcaggaga tacgatgccg tacttgctag cttcatgaca
    13261 gctttcctcg atcgtttcaa tcggcgcatt catcactgca accattttcc cgcttccagc
    13321 tggggtagct tctgtcatat aagcgcctct ttttgctact aacgcaactg cttcagtgaa
    13381 ggatagcgca ttactagcta ctaatgccga atattctcct aaactcagtc ctaaagctgc
    13441 atctgggatg attccatgct ccttcaataa gcgataatac gcgatgctga ctgttagaat
    13501 agctggctgt gtatattgcg tctgatccaa acgttcattt tctgtaaagc aaagttcagc
    13561 catatcgtag ccgagaatct cacttgcttc atcaaatgtc tgctttacaa cggcttcttc
    13621 atataaatct tttcccatac cttgatattg tgcaccttgg ccactaaata agaatgctgt
    13681 tttcatgttt ttctccaata tatccagaat atatgtttga ttcttcctat tcaaaaaggc
    13741 cgagacattc ctatttcagg aaatgtccca gcccccaacc taattttaat gattatgcag
    13801 tttgtttttc tacgtaagtc actaaatctt gaaccgtttt gattccgtct tctgtttcga
    13861 ttttcacgtc aaattcatct tcaatttcat tgatgatttg gaataaatcc aagctgtcag
    13921 cttctaagtc ttcttggatg tcagtagtca atttgatttc ttcagtttct tttcctaatt
    13981 cttctgcgat gatttcttga attttttcga ataccataat aatatattcc tccgatattg
    14041 tttgcttttt atttttataa tttaacagtc tacaactcca tgagaatact tccccatgtt
    14101 aaacctccgc catagcctgt aaatattact ttctgctgtt ttcctaaggt gatctttcca
    14161 ctagaaactg cttcatccaa taagataggg atgcttgcag cagatgtatt cccatattta
    14221 tccatattgg tcaggaactt ttcttgcggc atctttactt ttcgggcaat tttctcaata
    14281 attctgaggt ttgcctgatg caaaagcaga taatcaactg gtttgtcttt catgacttct
    14341 tggatattgt ttgcaacgtc tcgtacggca aaatcaaaaa tatctctgcc agtcatttgc
    14401 aaataatacg aaccttctga atcttctgaa tagaacggat ttttattttg ctggtagcca
    14461 gcggtcaatg agctacttct ggtaccgtca gctgctaatt tttctttcag caattttttc
    14521 tgtggattcg cttctaagag aatcccacct gctccatcgc caaacaacac agcagtcgta
    14581 cgatcagacc aatcgattag cttagatagt gtttcaccac caatcaccag tcccttagtt
    14641 gagccgcagc ggatcatttt ttcagccatg cttaacgcat aaacaaatcc tgagcacgca
    14701 gcactgatat caaaagcgat agcatgagtt gctcccaatt tcccttgtac ttgtgctgct
    14761 acagatggtg tcgtgtaatc gggcgacatt gttgctacta aaataaaatc caaatcttta
    14821 gggtttagat ttttctgttt tatcaatttt tccgccacta aaatgcataa atcagaagtc
    14881 tcctgatttg ttacacatct tcgttcacga atacccgtcc ttgacgaaat ccattcatca
    14941 gaggtatcca tcatcttgga aagatcatta ttagtaataa tcttttcagg aaggacgctg
    15001 gctgttgcag taatcattcc aaattgttcc attaaatatc ctcacttata gtcttgcaag
    15061 aagtcgtgca agtttttcag tgcatccagc aaggcatgct gttcctccgt agccattccg
    15121 tctaagatgt tcttgaccat ttcacgatga aaatgctgat ggacccgaaa tagtaacttt
    15181 ccttttttcg ttagtcccaa ctttacaacg cggcggtcat cttcactgcg gatacgttcg
    15241 acatagcctt tttttactaa cttgttaata gcagtggtca aggtcccaac agtaattgat
    15301 agttctttgg ctacttctga agttgttttt ttcttgtaca tcccgattgc ctcgatcgtg
    15361 tgcatctctg tgattgacag atctttgaat tgagattttt tcagctcaga ctcttcgatt
    15421 gtcaaaatat cattgaagac actaacaaga taatcattta ctgttttt
//
LOCUS       DAHHZJ010000053        15307 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.054, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000053 DAHHZJ010000000
VERSION     DAHHZJ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.054"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(820..1161)
                     /locus_tag="NBL02_001347"
     CDS             complement(820..1161)
                     /locus_tag="NBL02_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCD2572041.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1161..1391)
                     /locus_tag="NBL02_001348"
     CDS             complement(1161..1391)
                     /locus_tag="NBL02_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCD2572042.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1678..2016
                     /locus_tag="NBL02_001349"
     CDS             1678..2016
                     /locus_tag="NBL02_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572043.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2221..2460)
                     /locus_tag="NBL02_001350"
     CDS             complement(2221..2460)
                     /locus_tag="NBL02_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HCD2572044.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(2436..2783)
                     /locus_tag="NBL02_001351"
     CDS             complement(2436..2783)
                     /locus_tag="NBL02_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HCD2572045.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(3515..4096)
                     /locus_tag="NBL02_001352"
     CDS             complement(3515..4096)
                     /locus_tag="NBL02_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCD2572046.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(4279..4905)
                     /locus_tag="NBL02_001353"
     CDS             complement(4279..4905)
                     /locus_tag="NBL02_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCD2572047.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(4927..6258)
                     /locus_tag="NBL02_001354"
     CDS             complement(4927..6258)
                     /locus_tag="NBL02_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HCD2572048.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(6413..6814)
                     /locus_tag="NBL02_001355"
     CDS             complement(6413..6814)
                     /locus_tag="NBL02_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCD2572049.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(6900..7106)
                     /locus_tag="NBL02_001356"
     CDS             complement(6900..7106)
                     /locus_tag="NBL02_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572050.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(7201..8703)
                     /locus_tag="NBL02_001357"
     CDS             complement(7201..8703)
                     /locus_tag="NBL02_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HCD2572051.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(8721..9176)
                     /gene="argR"
                     /locus_tag="NBL02_001358"
     CDS             complement(8721..9176)
                     /gene="argR"
                     /locus_tag="NBL02_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HCD2572052.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(9274..9891)
                     /locus_tag="NBL02_001359"
     CDS             complement(9274..9891)
                     /locus_tag="NBL02_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCD2572053.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            10112..11485
                     /locus_tag="NBL02_001360"
     CDS             10112..11485
                     /locus_tag="NBL02_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD2572054.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            11560..12672
                     /locus_tag="NBL02_001361"
     CDS             11560..12672
                     /locus_tag="NBL02_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCD2572055.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(12744..14381)
                     /locus_tag="NBL02_001362"
     CDS             complement(12744..14381)
                     /locus_tag="NBL02_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCD2572056.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(14549..15157)
                     /locus_tag="NBL02_001363"
     CDS             complement(14549..15157)
                     /locus_tag="NBL02_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCD2572057.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         4224 a         3136 c         2676 g         5271 t
ORIGIN      
        1 atagaaggta ttgtaaataa taatattgac aaaaagtgac tcaaaataaa aaaaaactta
       61 cattatactg gaatgaactg tattgatagt gtacctaaaa gctctactga cataagatcg
      121 tgtaatcggt aaagtaattt tattaattat acgggatgta atcaattaga gaataaacta
      181 atcataaaag gtacttttac attcataatt gtcgctttga gattctactt tggttcacta
      241 tctgacccaa ttgcacattt tcgatcatta caagtattgg tgtcgttaga acaactaaat
      301 aaaaactagc ggtagatttt tcagaatcta tgctaatttc tgtatggatg ttccagtgat
      361 ggcaaatttc tcttttcatt gtttttcttg tcgcatgata tgcctttttg tgttattcag
      421 ttaaactata gaaattcttt cctagacaga tcttgatccc aaattttacg tttttgagca
      481 tcgatatagt ttaaaatgac attactcata tctaaagagt ttagattttt taggactaaa
      541 actgtcagtt gttctttttc ttctttctgt taaagtattg tcgcgacttt tcgtatacgt
      601 taagatatgt cgtgaaaaaa caagttttgt gacacaaaaa aacgaatgac ttgaggaaat
      661 cacctcaatc attcgtttaa aatgctatat attctttagt ttaagatacg gaaattaatg
      721 ccatcgacca cactagtaga atgaacgaca tttacttggc ctttttttaa tttcgttatg
      781 tttagcatat aaaaatatta tagagtttac ttacaatgct taaaacatat actgtataat
      841 ctgatcaatt ttagccatgt cttgcaatgg taagttttct atttttttga gttttcgttc
      901 cttaaaatca agagatttta attgtgtaat aattacttgt ccttttatat ctagctcttc
      961 aggtaatgag taacgtgtcg gtaaattctt tatagtggaa gtaattggac atatgaccga
     1021 aaacattgtt ttacggttaa attcataacg agagacgact agtcctggtc gtcttttttg
     1081 aatttctttt cctacggagg gatcaaaatc aatccataca atatcacctt ttttaggaat
     1141 ataaattctt tcttctgaca ctataaaatt tctctccctt caggagttaa atcttcccat
     1201 tcgtctttct cataatactc ggcctcttcc ccaccactaa atggatcttc aatttttgga
     1261 actaacgtaa ttgttccatc atcagaatac acaacgatat attcttggtt ttctgaaggt
     1321 ttctttccgt tatcagaagg taaagttacc actacagaac ttccttgtaa tctagatttt
     1381 gtaacaagca ttatcatcac tcccttaata aaagtatact atataacact aagtaacgca
     1441 aaattcccct ttgtaaacta tctagaaaat tgttttgttt aaagtatatc ttattggata
     1501 taaatagaaa taaattgtag ccagttcatt ttttctttaa ctaaaaaaat aatccaagaa
     1561 acattgaacc tatggatcca aaagctaccc aaatactata gctaaccccc cgctaccttc
     1621 aattttgcag ataaaacgct ttgggagaaa atttcaaaga caacaggcca tcctatcatg
     1681 cccgattata aacaaaaagc tggctattcc ttttgctctg acaaatttca attgggaaag
     1741 ttcacgaaac tagcaggtga agaagtttca actgttcgga tcaaagaatg cccgcttcaa
     1801 atagaaacat tggttcaacg aatttcaact agacaagact ttgctattgt agaacgtgag
     1861 atcgtagcta tttttgtaga agaagggatt ttacatgatc caactcatat caatgtggat
     1921 aaatggcaac ctcttatcta taaattcaga gaatatacaa ctgccaacca gtcgttaact
     1981 ttgaatttta gattccaaga attcaaagag tcataatgat aaacatcgtt aggtataaat
     2041 aaaaaagact cgcagcacga gtaaaaaaca aatagaagct atacattaga aaggtcgact
     2101 gtgaccctat agatgcagta tatgcagctt atgagcattt cttctgttct ctttgttttt
     2161 gggatctgtg ctgaagtctt tttcgttttt tgccgctctt tctttgaaaa atcgattatg
     2221 ttataccttc tccattacta attcaggcat ctgctgagta atgatttttc gcgcaaaaga
     2281 aatatctgtt acccaaggtt cgatttgttc ttcagtcaag acaagcggca ttcggtcatg
     2341 gattggtgcg actgtgcggt taggttcagt agtcattatg attgattctg ccattttttc
     2401 atcctgatgg ctacgataaa aaccgccaat atacactatt ttttccttat ttgaaaacat
     2461 aaatttgtct ttttcttcat tccactcatt gggaaaacac aacgattttg caaaaatggt
     2521 tgatgaaagg tctttttttc ttctactgtc tctgcacggg cattaatcat caattgtcct
     2581 tttttaaatc cggtaaatcc ccacttcatg atttccggag tgaggatgtt ttcttcctct
     2641 tttcttaacg tcacaacaag atttgacgga aaaacttctc cagctgctaa ttctgctgcc
     2701 tgcttcggtt gtttcgtctg ggcttctcga taataattct gaaggagctc tgactctaga
     2761 tcaaaaaaat aacgaccaca catcctaatc cctcccaatc tttttttcta tcatacactg
     2821 caacaagaag ttcgcaccaa caaaacgctt tcaatttttg tagaactaaa aaagttgtga
     2881 caagtgacaa actctccagt gaatggtatt ctcctgccaa ctcctcggtc acaagtcaca
     2941 atattcaaca aacatgagaa gctgcttttt gaaattgttc cttgtgactc ggagtgaaac
     3001 ggcaggctta cgacctctgg gtaaacggtg ttcaaaagcc agtttctcgg tattaagcca
     3061 aatttatgca aaaatgtaga aagctatttt cacaaaattt ttcttaatac tcgaaactaa
     3121 gcgacttttt cacaacctct tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa
     3181 gcccaaccaa acgaaaaata tgagcagcta tttttatttg gctgttctta ttacttgatg
     3241 cagggcagct ttttcacaac ctctccacta aacgatgttc aatcagctaa ccttcggtat
     3301 tagctcaaaa aacggcaaaa tatgaggaac tattttgccg tttgtttctc taatactcag
     3361 gtcaaagcgc tgatttcaca acctctaata cacggtgttc cagaagtagc ttcttcgaaa
     3421 aataagccga aatttcacaa aaatttgaga agcaattttc gtaaattcct tcttatttct
     3481 tgaagctgac cacttctgtc acaacctcta attattaaag aaccttttgc gaagttaaca
     3541 atctttcttt gatcaattga aaattctgcc taagtagtga tagatcctgt gtgactttca
     3601 acaataagat acttccctgg atttcagcaa tcaacgcttt ggcttgacgg tctgcttctt
     3661 catcggacag tttttcttca gaaagtgcca ggatattttg tttcgtatac ttgacccatt
     3721 gattgactaa ttctagtagc aacagacggt attcttcatc cactgtactc aactcaataa
     3781 tcaaattacc tacaggacag ccatgataag tgatctgtgt ctcatgatca gcgaaaatcc
     3841 agtccaacat ttcttctaat gcttcttctg gctgttttcc agcatttaag atgtcttcga
     3901 acaattctgt ctgccatacg tccatatgcg catggatcac cgctagacaa acttcttttt
     3961 ttgaagaaaa gtaataataa aattgacctt ttcctgtatc agaagctgct agaatatctc
     4021 ggattgacgt ttcatgatag ccttttttat aaataagatc tttggtcgct tccaagatct
     4081 tttcctttgt tttcaatgat attctccttt ttcatttctc atagtcctat attatacagt
     4141 aaattcatca ttttttcttc ttaatcgttt aaaaaagact gtgccaaatt atctgacaca
     4201 gccctcacta taaaactgtt ctcatatagc tagatgttca ttgattcaag aactgcgagg
     4261 acaaaagcaa ttcctatctt atgatagttg ctctaatact tcctctgttg tcatcacttg
     4321 atctgcaata aagccaaagt tcgtcaacgc tgcatccatt gcttcttggc caggagcacc
     4381 aattgcatct gtcgctacca caacattgaa tccactctct accaattcac gcatatggga
     4441 atcgctacac aagtttccgt tcattccacc aagaattact gtgtcgattc cattttttct
     4501 caattggagc gccaaatcat tgctttctgg tccaaagatt ttatgaggag agctgatgat
     4561 cgtattttca tccaaataag gtttcaattc ttcgataaaa tcggctcctg aaccttcttc
     4621 cactgattca tattgacttt ttctagcaaa catctgctgc tcaagcatca tatgttctcc
     4681 tgcaccgcca aattgccatt tatggtcatg tgggtaaaaa taatgaggag aaataaagag
     4741 tttgtatcct ttttcttttg cttgttttac catttcaatc atattttcga tcgtattgta
     4801 tttttctaag atatcttttg tcaaaccaaa gccaccgcct gctggtttca agaactcgac
     4861 ttgcgggtca gtgagaacta ctgctgtatt tttattattg aacatggtgg attctccatt
     4921 cgttatttat tttaatacat catttagagc ttcactcatt gtcggatgag aatagatttg
     4981 atctctcaat aacgtataag gaagttttcc attcatagct agcgaaatca agttgatgac
     5041 ttcatgagaa tcttccgcat agatacttgc ccctagaatc aagtcagttt cagggtcgac
     5101 taaaactttg aatacacctt taggatcttc taatacttgt gctttaggga caccgcttgt
     5161 catatacttg aataatttgt aattgacgcc ttgtgctttg gcttgttttt ctgttaaacc
     5221 tacattggat aaagtcggag tgataaatac actgtaaggc acagctttcc ggttattcgt
     5281 tgttcgttta ttctcgccct tcaactgatc cataatgatt cgataatcat ctagagagat
     5341 ataagtgaac tgcaatccgc ctttgacgtc tccaatcgcc caaatgttgt ctgcatctgt
     5401 acgaaggtat tcatccacag cgattgctcc gcgatctgtt agtttaacat ctgtattttc
     5461 taatccaagt gatgctgtac ttggttttct tccagttgca gccaaaactt ttgacgcttg
     5521 gattgttact tcttggccat cttctgtaaa ggtgacggca gctgttgatt cttggtctgc
     5581 tacttgatct acagatacac ctagatggaa ttcgattccc gcatttgtca tgtcttctaa
     5641 aatcatttgc gcgatatctt catcttcacg tggaagaaat tcagaatggg catcaagaac
     5701 gacaacttta gatccgtatt cattgaacat tgatgcaaat tccaagccaa tatatccagc
     5761 cccgatgatc actaattttt ccggtaattg ttcctgatcc atcgcttgcg tagaatcaat
     5821 caagtagcga cttgtattca atcctttgat tggtaaaatg attggttcag aaccagtatt
     5881 gatgaaaatc cgttcgcctt caacaatcac tttttcacca ttattcgtca cttcgatttg
     5941 atggttagaa agaaaacgtg ctgtaccgtc catcactgtc ccagttgcct catcgctgat
     6001 catatgataa tttttggcgc gaagcatgcc cgtcaatttt tctttccgag caacagcctc
     6061 tgtaaagtcg attcctcttt ctccattgaa aatcaatgat ttagaaggaa tacagccgat
     6121 gttgatacat gttccgccat acatacgtgt tgacttttca acgactaaaa ccgattcacc
     6181 ttttgaagct aacgccttcg ctaaagtctt accgcccttt ccaaaaccga tcacaatatt
     6241 ttgatatttt tccatcattt ttccctcgct tcttttttac ctaccgattg gtacaaaaat
     6301 atgataaccg ataattgtct attctccaaa tcatctgctt tatttgtcaa aaaaaaccag
     6361 acaaaaagcg gtcctttcac gtttttagtc tggttcgatt tcctttattc ttctaaacaa
     6421 tcgttacttc tacatcgata tttcctctag ttgctttgga gtaagggcag aattcatgcg
     6481 catcttctgc aattttttgg gcttcttctt cgctgatgcc atcgatatgc acttctaata
     6541 ctacaccgat ctgatagccg ccatcatcta aattataaag agagacacgt gcttttactg
     6601 tggactctgc tgtcattcct gcttctttga tcatatgttg taccgcccca ttgaaacaag
     6661 aagcataacc tgctgcaaat aattgttctg gatttgttcc ttctgcatgg attcctggag
     6721 gagtgatttt catatgcatc gagccatttg gtgcctcgac ttctccattg cgcccgccat
     6781 ggttgatcat tgctgtttcg tatgcttttt tcattgtact gctcctttcg attgaccatt
     6841 tgctacttgg ctataattat agtgtatgac aagtaaggcg ctttctacaa aaattctgct
     6901 tattcgaata ccagaatgcc gtcttctact atttttccaa aaaacaaaaa aatactactc
     6961 gtgaacaaca gcgtcatatt tgttccaaaa taaatagcaa tcttttctac ataatctttt
     7021 atatgattga ttttaagata catatatacc agaaagttga ccccataaac caatactgtt
     7081 aaaaaaataa ccatattcgt taccattttt tcctcctttt gctggtctcc ctaccagcac
     7141 tcgatttaac cataccactt tttttgccgt ccaagcaact agcctttcaa aaagactggt
     7201 ttattctgct gttaatgatt caagatcaga aaaatgtccg aataattttg ctaattttac
     7261 taacgccaac ttgttctttc cgaattcatc attttctatc tgtaatacta gtaacggctc
     7321 atgtaagctt aaacggatca aaaaccagcc gtttccgtat ggctcttcta gatcatatcg
     7381 aataccttct tcattttccg ggtcgaagtt catccctttt tcttcttcca acagtcgctt
     7441 catttcttct aatatcttgt ttccttgttc gcgtggttca tcgctgaaca attttaatcg
     7501 gatttcttgt acttctacgg gctgtttcaa ctctttgatt tgttcatcta atgtgatacc
     7561 ttttcttttt aattctggca aaagcatcag gatctttgcg atgacatatg caccatcatc
     7621 cagaaaatag ttttccttga atgcagcgtg tccgcttgtt tcgatggcta gctgtgtatc
     7681 tttcccaaga cggttaagtt ctaatgcttt attgattaca tttcgatagc cggaaatata
     7741 acgaacttgg atgcctcctt tttcttcaat gaatctttgt agatgagaag aagtagggga
     7801 attcgtcacg attcttgttc ctggatgctc ttttaatacg attgtactta atactgcgat
     7861 caaattattt cgattgatca ctgttccaga agcactgaca actgcagaac gatccacatc
     7921 tgtatcgaaa atgatcccaa gatctgcttc atttgctaaa acagcctgct tgatactttc
     7981 catcgcttct ttattatcag gatttgggat atgattagga aatcggccat ctggttccaa
     8041 aaattgggaa cctgttgaat ccgcacctaa gctattcaat actttttcag caaagaatcc
     8101 accagcacca tttccggcat caacaatgat tttccaacca tttagtggtt tgctttctag
     8161 tgttccaatc ccctttttaa tttttgtgac taaatcttgc gcataaggtg tcaaaaggtc
     8221 tgctttttct acgcttccag tttgttcgct ttttattttt tgctgctgtg ataggataaa
     8281 agcaatatct tctttttctg ctcctccttc tgaggtaaag aattttattc cattataata
     8341 atagggaaga tggctagcag tcagcataac cgcagcatcg cacgagaact gttcaaattg
     8401 agtggacata aacatagcag gcgtagtagc taattcgaaa tcgatgacat gtatcccttg
     8461 ttctttcaga ctttggatca acgcctcttt cagtgctggt ccgcttatcc tactgtcatg
     8521 accaatccca atcgtccatt ttttatttgg atcggttgat ttcttctttt tctttatcca
     8581 gtttgctagt ccccagccga tcttttttac catgtctgtc gtcaagttta cttcatatcc
     8641 ttcaactggt aatgcgattc ctcgaatatc cgatccattt tgcagcgctt tcatatcttt
     8701 catataaaag tcctcctcat ttattccctc attttttcca gcttttctgc aaatcgctgt
     8761 gcttcttctt ctgagcggca aataataatg aatgtatcta caccggccat cgttccgaca
     8821 acttcagggt aagccacttc atctagccaa ttcgttacca catcggcacc atttcgttca
     8881 gtcaatacga taaccatgaa ctgaacagac tggatacgca aaacctcgcg tctaattgct
     8941 gtacgcaaac gttcttcatt taaagaaaca gatggctggt taaataatgt atagcgtact
     9001 attttgtttt cattttgact ttttactaac ttcatttctc gaatgtctcg agaaatcgtt
     9061 gcttgagtca catcggcgcc tgcttcatgc agccgcgcaa gcagctcttc ctgtgtccca
     9121 ataggttgtt ctgtaatgat ctggcggatc agtgcttgtc gctgtttctt tttcatatta
     9181 gacacctctc aatgttgatt atagcaaaag ataagtaata tttcttcata ttttggtatt
     9241 ttttactttt attttttgtt ttcattcagg ttgctaacct ttttctattt taacacgcaa
     9301 aatcgtcttc attttttcag gtgctgattt tcttggatcg ctgatgtaaa tctctttgtg
     9361 ttctttagaa gctctttcat agccttcttt tgcgatgaat gcttctaatt tctcaaacgt
     9421 tgctggttct tcggcatagg gcccgacgtg taagatttgg gcacaaagac cttcttccat
     9481 ctttttgaat actacttgtt ctttcaagtc ttccggaagt ttgctagaac gctgaatggc
     9541 ttcttgtgct atcgcttctg taacaaagtc aggctgtcgg atcatcaatt gataagaaaa
     9601 atgttctttt ttcatcactg gttcatgcaa atacttttct tgcagtcccc attgtccttc
     9661 cagtggatag acagtataca tctgataatc aggaatcagc caattttttt taggcgacat
     9721 gcgtattgca tagctgactg catatagaca ggctactctt cgtttaaagt cttcctgatt
     9781 cgggtcgccg atccctctga tgctaaagaa tgatttttta ggaacctcta gtatttgcgg
     9841 gctttttttc atactatagc tcttgtcttt acgccattca attttttcca tttttcttcc
     9901 tccgcgaaat tccatttagt tatttattat catacctctt tttgagtgac ttggctaatc
     9961 gtgttgacac tatttttcag tttgtgctag aataagtgaa gtaaataaat cgtacagata
    10021 acacgtttcg gcgtgtcgag agagaggcgg tttctatttt tataatagaa atcactctct
    10081 ttttttacga aattttttag aggtgatgta aatgacaaaa caaaaacagc tgcttttagt
    10141 acctgcagta attgcaacag ggatgatgtc ttttgcgggc gtattgatcg agacggccat
    10201 gaatgtaact ttcccaactc ttatcagaca gtttgattta acgactgcac aagtccaatg
    10261 ggtaacaacg atttatcttt tgatgatttc gatcattgtt ccgctgtcta cttatttgaa
    10321 tcgcaatttt agtctcaaaa aactcttttt agcttcaaat ctgcttttct tggcaggtat
    10381 aatgatcgat ttcttctctc ctaccttcat ggtcctattg tttggtcgat tgctccaagg
    10441 tgcagcgaca ggtatcgctc ttccgttgat gttccatatt atcttgacat ttgcccccct
    10501 caatcgacga ggcgcgatga tgggtgtcgg tactttgaca acggccattg ctccagcaat
    10561 tggccccact tatggaggca ttatgacttc acattttaca tggaatcata tcttcctgtt
    10621 tcttgtgccc gttttgtttc tttctttgat tatcggctgg tttgctattc ccgagatggc
    10681 agtacaaaaa agtggttatt tggattggat aagcgtttta ggaatttgtc ttttgtttag
    10741 tggaagtcta acatttttga gtacgttggg aacagtttcc agctggattg ctttaatcat
    10801 cggcggtatt ggctggttcc ttttttacta tcgaagtaaa aaagtggagc atccactgat
    10861 ccgactgtct attctttcaa ataaaacatt ccgcttgttt ttgattggct tcttagttta
    10921 tcagttttta ttacttggtg tatcttttgt cttgccgaat ttccttcaga tcgttcaagg
    10981 aagtagtcct tttgtcgctg gattagcgat gcttcccggc gcagcggttg gtgctttgct
    11041 ttctcctttt tccgggaaaa tgctggatca atatggtccg aagaagccaa tttttgctgg
    11101 gctgattctt tctttgattg gttggctagc attattccta ctcataggac acgcttcagt
    11161 gggtgtattg attgcttgtc atgtgtttta tatgatcggt atcggattat cttacagcaa
    11221 tatgatgacc accggaatga atgcactgaa tgaagaacta caaggagacg ggaacgcaat
    11281 gttcaatacc ttgcagcagt tttctggcgc tgtggctact tcattagtag cggtcattat
    11341 caattacgtc cagcatcata cttcccataa ctatgaagtg tctactacat taggatcaaa
    11401 agcagcatta ggcgtcttgt tgctgttgct tctcgtaagc tttgctcgat tcgcctatta
    11461 tctgttcttt gctaaaaaag cgtagattcc atatacggca ggtgttgcat ctgccgtttt
    11521 tttgtgtgaa aatggttgtt tgtaaaagga ggctttttta tggaaaaaat tgccattatc
    11581 ggtggcggga tcattggtat gaccctagcc agttacttag atacatcaaa attcaatgtt
    11641 gctttatttg atgaagggac aggacaggca actaaagcta gcgctgggat tatctcccct
    11701 tggttgtcga aacgcagaaa caaacaatgg tatcgtctgg ctaaagacgg cgctgctttt
    11761 ttccctaaac ttataaaaga tttccaactt gatcaaacga tttacgcaca atcagggaca
    11821 cttttgcttc gacctgaaaa agatttagct gaattggctg ttcttgccga gaaacgaaaa
    11881 gaagaagcgc cagaaattgg aaacatcgaa tggctttcac ctgaacagac atctcaagca
    11941 cttccgttat tgaaaccagc agtttcactg cgtatcagtg gtggtggtcg tctggatgga
    12001 aaagcatatt tagaacatct tggtcagcgt ttagcacaaa aacaaatgga gcagatccag
    12061 gcaaaagtca ctttgcataa gaagagcgat tcttggctca tcaagggacc gtttggagaa
    12121 tacacagctg atcgtgtaat tttgacacct ggcccggcac ttaagccttt attacaagaa
    12181 ttaggctatg ctgcagatat acgtccgcaa aaagggcagc tcttagcatt tcagacatct
    12241 tataagatga gtcaagactg gcctgttgct atgcttgacg gagaagctga tttcattcct
    12301 ttcatggatg gaaagatcct tttaggtgct acccatgaaa acgatcaagg ctgggatttg
    12361 gctcctacta gcgaagctta taagggattg actaagagta cagaacagtt cttgactgat
    12421 ccagatcaga taaaaaactg gccatacgag ttccgtgtag gtacccgtgc ttatacgtca
    12481 gatttttccc catttttcgg cgtactgcca gatgatccaa gcttactggc tgctagcggt
    12541 ctaggctctt ctggcttgac cacaggtcct tatatcggct atctattagc caacttctta
    12601 aatactggtc attggcctac ggaagattat caaaaggaca tctccaccta tattacgaaa
    12661 aatccatcat agaaaaacag ctttttcaag taaggatccc gagcgatatt gcgcatggat
    12721 tacttcttga aaaagctgtt attttagttc aatttctgaa tcaaattctc caaactttct
    12781 tttgcttcat ctggcaagag atcttttcct atctgagctt caatgaatcg taaacgaata
    12841 tcaagtcgtt gtttcaccag atttttatag gacgtatcct caaaagaaac aggaaaatct
    12901 ggtataggta aatagtggat gtcttcaaat ggaccaaacg acttgaattc tgccgtttgt
    12961 tgcacgattc tttcaacgat tgacaatgtc gtttcaggtt tgattttttt atcatcgaaa
    13021 tatccagtat tcaggacata gcaggctgtt tgctgtttca aaaacaactc tcgaaaagct
    13081 tgatagtctt ccctaagagg atagacacgg aaaggattag caaatggttc aatgactagc
    13141 tcatctagct gtctttttgt agtgatattt tccgcgtctg atcgttttgt tgctaatgtt
    13201 gcaccaaaag acgctgctaa aactgggtca ttgatcttga taattggcgg aagcgaatca
    13261 tctttcatga tccaaaaaac agcatcgatc ttttcgctaa gatgatcgac tcggtcgggt
    13321 gtcgcataac gtgatttgac tgtccgccca tttccgtttc gtatatctcc tgttactagg
    13381 actttttctc ctttacttgt acatgtcacg cccacattct gacaagtaag gaaagaagaa
    13441 atggccggat catcagacgg atagtcttgt gttttatcaa agtaagaagg ttccaaggca
    13501 gtagcaaaac cattttttcg agaaataacg aaagcatcgt catgcaagat cgaaacagag
    13561 tgaccttccg tatgttttgc taaggtaatc gttgattttc cagaaccgga caacccaaat
    13621 acagccatcg tataggtctg gtcattacta gcatattgtt tcatcccacc gtggcaggca
    13681 ataaagccat tgcgatttcc caatgcccaa gcaagcgtca aagtcgcttt ttttaattct
    13741 ccaaagtaac gtaatcctag cacagccgct gtattatgga gagcatcaaa caaaatgagt
    13801 ccatttggaa atgtttcatg tgtccattcg ggatcacaaa agatataaag atctccctca
    13861 tcaaaggaaa cagacttttg ataagtctct aagatcttcg aattcgctgg ctgaaaattc
    13921 agcaaataag aatacaaatt gttctcaaat ccttctggaa tcagcaaatg actttgcaca
    13981 gaaaattctt cagataggcc aacgattact tttgtatggt aaaactggcg cttgcgactt
    14041 tgatacacag cttctcttaa aattttctcg tatttgcttt gttccttgtc taagtcctca
    14101 aagatccgcc gagcacttgc tgttcttccc acaatgcttc catcattgaa tactagtgtc
    14161 ctaacatcag ctggtaaatc tagttcttca gcatgtgaga ttgttaaatc tgtgatgatc
    14221 gttcctggac tcccttttgc tagttgataa gctgtcttcc tatcaaaaac ttcatgaaca
    14281 ttattaccgt aaaatgtcgt ttctacaatc gatcgaatac tggataacag tggatttcca
    14341 cttgtgattt cgtttaattc gagactctct tttgtactca tggctcctcc tcattcccta
    14401 aacaaaaaaa cgttttcttt attatacatt attcgagttt ttatttgtac tgctgcgctg
    14461 tgggaagcaa acgagttatt tttcacacaa aaaaaccgag aacttgaata aacaagtttc
    14521 tcggtttcat cattttcctt ttatcggctt agtaccagcc gtttgctaac cagaatgttt
    14581 tagcagcatc ccatgaacca taacgagatg ttacatattg ttctgctaca cgttcttgat
    14641 tggcagctga atagtcgccg tttaagtatg aagcatctaa ttgataacgg ccgatgtaac
    14701 gtccgttagt agctgtgtat gaaccgcctg attctttttg tgcgatccat tcttttgcag
    14761 agcttgaagt tggcgctgtt gcagcagctg gggcagcagg agctacttca actgtttcaa
    14821 caggtgcaac gtatgtatgt tcttgcgttg ctgtttcagt tgtagatgct gtattttctg
    14881 ttgttttgtt ttctgttgtt gctgaagaat catttgaagt tgggattgtt aattgttcac
    14941 caacataaat gcggttgatg tttgcgatcg aatttttttc agcaattgca tcgatcattg
    15001 agttgtctcc tgcaaatttt tgtgagatct tagataatga atcacctgat tttactgtat
    15061 aaacttcatc agcgtgagca tttgtagttc ctacaaacat tgcagtagca ccagcagcaa
    15121 aagttgtacc taaaacaatt ttcttaaatg aattcatatt aaactccttt ttttctccgt
    15181 tttttattct ttcgacgcta ttaactttat catttagaaa tatttcatag gtaaaagaaa
    15241 catgacattt aatgacaaaa taacgaatta tttaatattt gtaacattta aacaaatatt
    15301 tttttga
//
LOCUS       DAHHZJ010000054        15217 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.055, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000054 DAHHZJ010000000
VERSION     DAHHZJ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.055"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(24..722)
                     /locus_tag="NBL02_001364"
     CDS             complement(24..722)
                     /locus_tag="NBL02_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HCD2572058.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="NBL02_001365"
     CDS             complement(715..2067)
                     /locus_tag="NBL02_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HCD2572059.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="NBL02_001366"
     CDS             complement(2290..3690)
                     /locus_tag="NBL02_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HCD2572060.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="NBL02_001367"
     CDS             3851..4588
                     /locus_tag="NBL02_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCD2572061.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="NBL02_001368"
     CDS             4713..5192
                     /locus_tag="NBL02_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HCD2572062.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="NBL02_001369"
     CDS             5189..5956
                     /locus_tag="NBL02_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572063.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="NBL02_001370"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="NBL02_001370"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCD2572064.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="NBL02_001371"
     CDS             complement(6859..7461)
                     /locus_tag="NBL02_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCD2572065.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..8036
                     /locus_tag="NBL02_001372"
                     /pseudo
     CDS             7567..8036
                     /locus_tag="NBL02_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8142..8726
                     /locus_tag="NBL02_001373"
     CDS             8142..8726
                     /locus_tag="NBL02_001373"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCD2572066.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="NBL02_001374"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="NBL02_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCD2572067.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="NBL02_001375"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="NBL02_001375"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCD2572068.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="NBL02_001376"
     CDS             10627..10806
                     /locus_tag="NBL02_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572069.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="NBL02_001377"
     CDS             10828..11853
                     /locus_tag="NBL02_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCD2572070.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12028..13272
                     /locus_tag="NBL02_001378"
     CDS             12028..13272
                     /locus_tag="NBL02_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCD2572071.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13425..14372
                     /locus_tag="NBL02_001379"
     CDS             13425..14372
                     /locus_tag="NBL02_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD2572072.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14506..15135
                     /gene="upp"
                     /locus_tag="NBL02_001380"
     CDS             14506..15135
                     /gene="upp"
                     /locus_tag="NBL02_001380"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCD2572073.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
BASE COUNT         5188 a         2720 c         3111 g         4198 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaaaggagct gttgtttgtg gtagattaca aaacgtttga ccctgatctt
    12061 tgggctgcga tcgccaaaga agaagaaaga caagaacata atttggagct gattgcctct
    12121 gaaaactttg tctccgaagc agtgatggct gcacaaggaa gtatcttgac aaataaatac
    12181 gcagaaggtt atccagggca tcgctattat ggtgggtgtg aatttgtcga tatcgttgaa
    12241 aatttagcga ttgaccgtgc aaaagaatta ttcggagcga aatttgcgaa cgtccagcct
    12301 cactccggct ctcaagccaa cacggctgct tatctcgctt tggtagaacc aggggacacg
    12361 atcttaggaa tggatctttc cgcaggcgga catctaacac atggatcacc agtcaacttc
    12421 agtggcaaaa cctatcactt tgttgcttac ggtgttgatc cgacaacaga agttattgat
    12481 tataatgtgg tacgtatttt agctagaaaa catcaaccaa aattgatcgt agcaggtgcc
    12541 agtgcttacg gacgaacgat cgactttgcg aaattcagag aaatcgcaga tgaagtaggc
    12601 gcaaaactga tggtagatat ggcacatatc gcaggattag tagcagcagg acttcatcct
    12661 aatccagttc cttatgcaga tatcacaacg actaccactc ataaaacatt gcgtggccct
    12721 cgaggcggca tgattttgac aaacgatgaa gcattagcaa aaaaaataaa tagtgcggta
    12781 tttcctggta ttcaaggcgg ccctctagag catgtgattg cgggtaaagc agtagcattc
    12841 aaagaagcat tagatcctgc atttaaagaa tatagtgaac aaatcatcgc caatgcaaaa
    12901 gcaatggtca aagttttcaa tcaagcaatt ggaaccagag tcatttcagg ggcgacagat
    12961 aatcatctga tgttgatcga tgttcgcgag ttaggtataa atggaaaaga agcagagagc
    13021 attttggaca gtgtgaatat cactgtaaat aaaaactcga ttccttttga aacactaagt
    13081 ccattcaaaa caagcggtat tcgaatcggc acgccagcaa tcactactcg cgggttcaaa
    13141 gaagaagatg ctgtaaaagt ggctgaacta gttgtcaaag cattacaagc aaaagatgat
    13201 aacgctcaac tagacgaagt caaaacaggt gtacgcgaat taactgaaaa atttccgttg
    13261 cataaaaaat aaaaatttaa attccattag taaacagaaa agttcaatgt tgggcttttc
    13321 tgtttatttt ttgggaaaac ggattctttg acaatatcgg tgatgctctc tagtatatga
    13381 ggtgtaattt tagtcttcac actaaatctc agggaggaaa agtcatgact gatcgtatct
    13441 cgttagaaaa ggaagcagta gcattcagtg atgcaaatgc gcctcatccg cgtatctatg
    13501 aactagcacc ggaagaagga agagttttac tagaagaagt acaagaatca cctgtgaaca
    13561 aatattccgt cgatattgaa gacttgatgg tggatactgg cgaatgggga aaactcaata
    13621 ttcgtctcgt acgaccagaa gggaatatgg ataaattacc agttattttt tatattcatg
    13681 gagcaggctg ggtttttggc agtgctcaaa cccatgataa attgatcaga gaacttgcgg
    13741 ttcgtacgaa ttccgtcgtt gtctttccag aatatagtcg atcaccagaa gcaaaatatc
    13801 caacagcaat tgaacaaaat tatgctgtgt tgcaacagct gaataaatta gccaagtcaa
    13861 aaaatctgga tctcgagaat ctaatcgttg caggagattc agtaggtggt aacatggcta
    13921 cagtcatgac gatcttaaca aaagaacgaa agggcctccc aatatccaag caattgctgt
    13981 attatccagt cacaaacgct gattttgata cagattccta tcatgaattc ggagaaaact
    14041 attttttgac aagagaagga atgatgtggt tttgggacca atatacgacg aatgaagaag
    14101 agcgtgctga aatcacagct tcgcctttga gagcaaatat aaatgaacta aatgatcttc
    14161 cttcagcaat gattttaacg ggagaagcgg atgtattaag agatgaaggt gaggcttatg
    14221 cgcgcaaact tcgtgatgca ggggttcctg ttacacaagt acgcttccaa ggaatcatcc
    14281 atgattttgt catggtaaat gctatggatc agacaaaggc aacgcgtgca gcaatggcat
    14341 tatctacttc ttggatcaag aaaaaagaat aagctttgct tgtccgcagg aaaacttctt
    14401 ttcaaaaacg gattttctta ggaaaaatgg cgagtttgac tagtcaatca cttgattttg
    14461 ttttacaatt aaaaagaaac gacagaacaa aggagagctt aaaaaatggg caaatttcaa
    14521 gtgatcgatc atccattgat tcaacacaaa ctaactatta ttcgggacaa aaactgcgga
    14581 acaaaggttt ttcgtgaagt agtcgatgaa atcgctatgt tgatggcata tgaagtatca
    14641 cgggatatgc cattggaaga catcgtgatc gaaacaccga tcactgaaac aacacaaaaa
    14701 acgttgtctg gtaaaaaagt agcgattatt ccaatcttgc gcgcaggtat tggaatggtt
    14761 gacggtatct tggaattgat tccagctgct aaagtgggac atatcggtct ttatcgagat
    14821 gaagaaacat tggaaccaca tgaatatttc gtcaaacttc cagaagacat tgatgcacgt
    14881 cagttgtttg tagtagatcc aatgttggca acaggcggat cagcaatcat ggcaatcgat
    14941 gcattgaaag aacgtggcgc gtcgaacatt aaatttgttt gtttggtagc agcacctgag
    15001 ggagtaaaag ctttgcaaga agcgcatcct gatattgaca tctatacagc ttctctagat
    15061 gaaaaattga atgaacatgg ttatatcgtt cctggactag gggatgcggg tgaccgtttg
    15121 tttggtacaa aataagtaaa tacgaaaaag ggtgtgacac aacttttgtc acatcctttt
    15181 ttggtcattt ttaacgtttg atgcagaaca gcttttt
//
LOCUS       DAHHZJ010000055        15189 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.056, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000055 DAHHZJ010000000
VERSION     DAHHZJ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.056"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..1271
                     /locus_tag="NBL02_001381"
     CDS             <1..1271
                     /locus_tag="NBL02_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2572074.1"
                     /translation="KDATDRASKETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIK
                     KISQTDSIDSYVKRINSVADLVDYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTK
                     ETKFVSEAYKLVEGKHLENEDKNKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGA
                     DETVEVEIKGLFDGHNSGVVSAAQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFV
                     KGDKNLDSVIKDLGKLDINWREYNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAG
                     VVVSLLLFLWMNARKKEIAVLLSLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTA
                     DKLGNNILNKVTGDIAKQIAKQSASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVI
                     FMSLVLLVSLIISSFNILRRNPKELLIDNN"
     gene            complement(1557..3179)
                     /locus_tag="NBL02_001382"
     CDS             complement(1557..3179)
                     /locus_tag="NBL02_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HCD2572075.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYSFEEKIP
                     PFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANANKHKFT
                     KEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPESGYYVK
                     GEREKQFAYSVHTSCDDNGFILSTIITPRNIHDSQVAFQLVKQSKRLFPEINCVVADA
                     GYKTPKFVHFLTHLNLRPCLPYSRPKGKKELLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTREGYREYKSNPKECVNCPLLNQCTISKNHQRVITRHVWGDLMDEVEHLRLTDLNKS
                     IYKKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKE
                     PLFFCSKIRNEVDKKLFQARVTSLEQLLSTVCDVGDISFSIRNQLRFKRSLPVSGNIN
                     VYFTKARFKRLIAITVSSIVMFLWLFRMGVIP"
     gene            3831..4802
                     /locus_tag="NBL02_001383"
     CDS             3831..4802
                     /locus_tag="NBL02_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCD2572076.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            complement(4880..5116)
                     /locus_tag="NBL02_001384"
     CDS             complement(4880..5116)
                     /locus_tag="NBL02_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCD2572077.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(5085..5216)
                     /locus_tag="NBL02_001385"
     CDS             complement(5085..5216)
                     /locus_tag="NBL02_001385"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCD2572078.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            complement(5292..5675)
                     /locus_tag="NBL02_001386"
     CDS             complement(5292..5675)
                     /locus_tag="NBL02_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCD2572079.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            complement(5708..6493)
                     /locus_tag="NBL02_001387"
     CDS             complement(5708..6493)
                     /locus_tag="NBL02_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HCD2572080.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            6671..7771
                     /locus_tag="NBL02_001388"
     CDS             6671..7771
                     /locus_tag="NBL02_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCD2572081.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            7761..8585
                     /locus_tag="NBL02_001389"
     CDS             7761..8585
                     /locus_tag="NBL02_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HCD2572082.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            8582..9397
                     /locus_tag="NBL02_001390"
     CDS             8582..9397
                     /locus_tag="NBL02_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCD2572083.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            9394..10224
                     /locus_tag="NBL02_001391"
     CDS             9394..10224
                     /locus_tag="NBL02_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2572084.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            10231..11262
                     /locus_tag="NBL02_001392"
     CDS             10231..11262
                     /locus_tag="NBL02_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2572085.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(11369..12642)
                     /locus_tag="NBL02_001393"
                     /pseudo
     CDS             complement(11369..12642)
                     /locus_tag="NBL02_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            12919..13128
                     /locus_tag="NBL02_001394"
     CDS             12919..13128
                     /locus_tag="NBL02_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572086.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            13178..13894
                     /locus_tag="NBL02_001395"
     CDS             13178..13894
                     /locus_tag="NBL02_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HCD2572087.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(13796..14316)
                     /locus_tag="NBL02_001396"
                     /pseudo
     CDS             complement(13796..14316)
                     /locus_tag="NBL02_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            14604..>15189
                     /locus_tag="NBL02_001397"
     CDS             14604..>15189
                     /locus_tag="NBL02_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD2572088.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKEL"
BASE COUNT         4533 a         2739 c         3323 g         4594 t
ORIGIN      
        1 ttaaagatgc tacggacaga gcttcaaaag aaacatttgc taatataaca aatagttttt
       61 ctatggagat aaatagacaa gtaaatccag gaacacctag aggtggggga aatgtaaaag
      121 gtgaagatat taaaaagata tctcaaacag atagtataga ctcctatgta aaaagaataa
      181 acagtgttgc agatttagtt gattatgata ttattgaaac tcaagagact cttgcgaatc
      241 aatcacctga aagagcaaag aattttaaaa gaacagttat gttaactggg gttaacgact
      301 caacaaaaga aacaaaattt gtatcagaag catataaatt agtagaagga aagcatttag
      361 aaaatgaaga taaaaataaa atcttaatgc ataaagattt agctgaaaaa aataacctta
      421 aagttggaga taagataaaa ataaaatcta atttatttga tgctgataat gagaaaggtg
      481 cagatgaaac agtagaagta gaaattaaag gtctattcga tgggcataat agcggtgtag
      541 taagtgcagc acaagaacta tacgaaaata cattaatcac tgatattcat acagctgcta
      601 aagtttatgg taataccgaa gatacagctg tataccaaga tgcaacattc tttgtaaaag
      661 gtgataagaa tcttgatagt gtaataaaag atcttgggaa attagatata aattggagag
      721 aatataattt gattaaaagt tcatcaaatt accctgcatt acaacaatct atatcaggta
      781 tttactcaat ttcaaataaa ttattttttg gctcattaat atttgcagga gttgtagttt
      841 cattattatt attcttatgg atgaatgcta gaaagaaaga aatagcagta ttactatcat
      901 taggtatatc aaaattagaa atttttggtc aattcctaat tgagatggta tttatatcaa
      961 tccccgcatt cgttggttcc tatttcttag ctcaatatac agctgacaag cttgggaata
     1021 atatattgaa taaggttact ggtgatatag ctaaacaaat agctaaacaa tctgcatcta
     1081 gccaattagg tggtggggca gaagctgaag gatttaacaa gacattatca agtttagata
     1141 tcaatgtatt acctaaattc atgatttatg tagttatatt tatgagttta gtacttcttg
     1201 tatcattgat tatttcttca ttcaatatat taagaaggaa tccaaaagaa ttattaattg
     1261 acaataatta aaattttcgg tgctttttag caccaaaaat gagagtgtaa tataaaccgt
     1321 gtaagtaacc atcaatccta ggcgggtggt tgcttcacgg tttttatttt tctaaaataa
     1381 gatcaagagg tgattacacg atggctagaa agaaaagaaa tcctgatgct gagaagttag
     1441 ctgaatccat tttgaatact tatcaacccg aatctgtaga ggatatgcaa gatgttttaa
     1501 aagatgtgtt tggtccctta tttgaaaaaa tgcttcaagg agaattgaat aaccatttag
     1561 ggtatgacgc ccattctaaa gagccaaaga aacatgacaa tcgacgaaac ggttatggca
     1621 ataagacgct taaaacgagc tttggtaaag taaacattga tgttcccaga gaccgggagg
     1681 cttcttttga acctgagttg attcctaata gaaaacgaga tgtctccgac atcgcagact
     1741 gtagacaaaa gctgttccaa actcgtaact cgagcttgga acagcttttt atctacttcg
     1801 tttcgaattt ttgaacaaaa aaataaaggc tccttgcctt tccatagcca ggtggctaat
     1861 ttctttaaat tcattgcagc aaacactagc atcgtatgcg tcgcgacttt ttccaagcct
     1921 cgatacttgg tccaacgcat cccatgcttt tcttttgcat ccgcaaatat tcgttcaatc
     1981 gtttgtttcc gttttttgta aattgattta ttcagatctg ttaggcgaag atgttccact
     2041 tcatccatca gatctcccca aacatgtctc gtgatgactc gttgatgatt tttacttatg
     2101 gtacattggt ttagcagagg acagttcaca cattcttttg gattggattt gtattctcgg
     2161 tacccttctc gagtaaccgt tgaaaaagcg agcatttgat cttgcggaca aatataacaa
     2221 tcaaaatact cgtcatagag aaactcattt tttgataata gctccttttt ccctttgggc
     2281 cttgaataag gcaaacaagg tcgaaggttc aaatgagtca aaaagtgaac aaacttaggt
     2341 gtcttatacc cagcatcagc gacaacacaa ttgatttcag gaaaaagtct cttcgattgt
     2401 ttgacgagct gaaaggcgac ttgactgtca tgaatatttc taggagtgat gattgtgcta
     2461 agaataaatc cattgtcgtc gcatgaagta tggacagaat aagcaaattg tttttctcgt
     2521 tcgcctttga cgtagtagcc gctttccgga tcagctttac ttatttttct ttctttcagc
     2581 ttcacttcct tttctaagtc caaagtaaaa gggcgttttc cagcctctat tcgttgacga
     2641 ttgatctcat cttctaattc atcctgatag gctttcgctt catcatgggt catttctttc
     2701 gtaaacttat gtttattggc gttagctttg atgtgggtag aatccagata caaattatcc
     2761 tctgttacaa aacctgcttt aactgcttgt tctaaaatat aggcaaaaat atcctcaaac
     2821 accgtcgttt ctctaaatcg gcgaacataa ttctttccaa aagttgagaa aggggggatt
     2881 ttctcctcga aggagtagcc taaaaaccag cggtaagcga cattggtatc gacttcctta
     2941 atggtttgtc gcatggaacg gataccgaat aaatactgaa taaacaccaa tttgatcaga
     3001 atcactggat cgatacttgg gcgacctaaa tcggagtaag tggcttcgac gattggataa
     3061 ataaattcaa aatcaagcgc ggcatcaatc tttcgaacga gatgatcttg aggaactaga
     3121 tcatcaatcg taagcatcgc aaattgattt ctaccattcg tattgttttt agacatcata
     3181 gctatcactc cttgcattcg ttttcataaa tctattataa aagaaaagac tgtagacatc
     3241 atccataaaa tggactttgt ctacagtctg gataacgtga ttttttgctg taatgacaag
     3301 gttttagctg tttcagatac catgtatcca aaaatagttt attgagaaaa taggagagta
     3361 tttgatacta actagtagga aaaaatgaga taaaaagtaa atggaatcat ttaaggtaat
     3421 ctgaaataaa aaatatacag ccctgtggta ctttacctac aaggctgtat atatttttta
     3481 ctctcagcgt ttttgactcc gtgtctgact ccgttttatt gattttgaat gttattcaat
     3541 gaccatttaa caggtcaaat ttgatgtttt tgattccgta tgacgccgtt tgatatctag
     3601 tgatatagtg atttgagtct acatcgaata agggaataaa ccaataaaaa atgatttaga
     3661 atattaaaat aacaaaaaag aggactacct gtattagtcc tcttttttga ccagattttg
     3721 accagtctat tttacatcct agtatattta tatgagctaa ttttatgatt aaatggtaag
     3781 tttttccttg tgtgttttag tttaaactag tgtgtaattc aaaaatctat atggaaggaa
     3841 cagtattaag aatagaacag ggttctctac atgatggtgc tggtttacgg accgttgttt
     3901 atttaaaggg ctgcccatta cgctgtgcct ggtgttcgat tcccgaatca caaagcaagc
     3961 agattgaaaa aggatttggc cagaccatga ccgctgaaga ggtaatggat gagattgaga
     4021 aggatgctgt tttttacttt cactcggatg gcggagtgac cattagcgga ggcgaggcac
     4081 tggttcaggc ggattttgct aaagaaattt tgcaaaaatc gaaatacatt ggaatcaata
     4141 ccgtactgga aaccagcttt tgtggtgctt ataatgaaat ccaaaaggta gctccctatg
     4201 tggatacatt gtttgtggat gtaaaaatgt ttaccagcaa gctccacaaa caatggaccg
     4261 gcttagataa ccaacaaatt ttgaagaata ttcgtcgctt tttaattgag tatcctaact
     4321 gcgaagtgcg aattcgagtt cctgtggttc caggtatcaa tatgaacctg accgagctgc
     4381 tgacgattgc ttgttttgtg gcagatcttg atcggttcgt accgttagaa ttactaccgt
     4441 accactgcta tggtatgcac ggttaccaag cattgggact ggagtatccg ttggcagaca
     4501 cgccagcgcc ttcacctaaa gaaatgtttg cattggcaga tcaactggct cgcacggttc
     4561 ctcatttgct ggtcacagct ttaaccaaaa catttattta tttattgaat ccaagcttta
     4621 tcttatggat aaatgaaaga cactcactag ctgcagccag ctcaagaata gagcttttgc
     4681 tgaagtcagt gagtgtcttt ttacgatgtt ttttcgaagt taatttgtcg aatgaacact
     4741 ggctcaatcg gtacatcttc actgtttgtc ggctgagctg cgatcgctgc tacgatttct
     4801 aacccttggt atacctgtcc aaaaacagta taacgaccat ccagctccgg tagtcctcca
     4861 agctccaagt atgcttgctt tacattagca ggtcagcctg cgcctttgtg ttttgcacaa
     4921 taaaaaactg gttgccgttg gtgtgggggc cgtggtcccc taaggccaag gcgccattaa
     4981 aatgataaag tccgcgatgc acttcatcag cgaaaaagcc ctcatagctg gaaacagctt
     5041 catcaccaga accatccaaa gcgccacctt gaataacaaa ttccttaatg acacgagccg
     5101 aaaggtgtgt tgtcataaac gccttgctca gctagggagc gccagttggc tacagccagg
     5161 ggagcttggt caggaaataa gcggagacga atttcaccag catcggtgac cagcacacaa
     5221 acttcttcct gctgcggttt ttctaattga aagagaggaa ggtttgcaac tgaaacgcct
     5281 tgatcctgcc atcaaatatc gaaaatccgc cactcgacct tcgcctgatc accaaggcga
     5341 gccattccca ataaaacggt ataggcggtt tcatccgctt gaaaggtcaa ttgcagctgt
     5401 cctcgtaaat cgttgtagtc atctggggcg gctacttgat aaaaaagatg aaactgcgca
     5461 tgcaaatatt ccaaaggctg atctttaaag gctgcaaaat aagcctgaac tttttcccgc
     5521 tgcaagctgt cagccaccaa aaaattttcc agttgttgtt ttggaacggc tgctaacaag
     5581 gatcggcaaa ccgcctcaat tttcaacgca tcccaatgtc gcgcaattaa ataatggatg
     5641 ggtgcctctt gattgtagtc ccacaggttg ctcatttttt tcaccacttt cttctattat
     5701 ataggaatca caaaatcggc agcggcgtca ggcggcgttc ttttccttga atgactagca
     5761 acttttctac ctgacagtct gccaacagct gactcatttt tttaaagccc agctgataat
     5821 cttcggcaac atgagcatcc gagcctaagg taaagagctt tccgccgaca ctttgataca
     5881 aaggaacggc ataacggtaa aggtcttcgt tgccgtattt caagaagctc ttggcattta
     5941 actccatcgc caattctcgt tcaacaacct ttttgaaaat cgttagcagc tgagcctcaa
     6001 aatgctccgc gaattcttcc gctgtgaagg tgaagcggcg taatccataa tcaaagtgag
     6061 tcaaaatatc tccttctgga aaatccgtca agacctgcaa catctgatca aaatattcct
     6121 tggtagtttg aatgggatct ttggttaaaa ccacatcgtc catataatca aagcggccat
     6181 tttgatgaat acttaccagc tttaaatcat aaggatgctg agcgaggtaa tccacgatcc
     6241 gctcttcttg tcctggaaca acgccaatct cgatacctcg cagaaatttg gtggaatagg
     6301 tttcttttaa agagtccagt ttgtttatat aggctggata atccggaatg tcatcttttc
     6361 cggtagatgg attgtttaaa tcaaagtgat ccgtagaaac gaaaaactcc ggctgatagg
     6421 ctaaatagtt ttcaaatttt tcctctgaat caaaggaaaa atacgtgtgg agatgctgat
     6481 cataatactg catagggagc ctcctttttt ctactatcat accgcaaaag ctttagaagg
     6541 ttaacgggaa ttcgtcgaaa tttacaaacc atatacaacc tttacattgt tcatgaaacc
     6601 ttttcaagaa actgattctc tcattacaaa gctttgttag aatggaatta tcgaaagggg
     6661 agaagaatga atgaaaatga atgaaaaaat tcgtaacatg actatcggtt tattttgtgc
     6721 agcaacagta agtatcctgc tgacaggctg cggctcaggt gaagcaacga agcaatcggc
     6781 tgcggcagac gactctttag atacgattat agaaaaagcc aaggaagaag gagagattgc
     6841 cagcttggga atgccggata cttgggcaaa ttgggtaggc acttggaacg accttgaaaa
     6901 agaatacggc ttaagtcacg tggatacgga tatgtccagt gcagaagaat tggctaagtt
     6961 tgaatcagaa ggaaaaaatg gaaccgccga tattggtgat gtcggtatca gcttcggtcc
     7021 gttggctgaa agcaaagagt taaccttacc ttataagacc aactattggg aggaaattcc
     7081 ggattgggca aaggatgatg atggggattg gttgttaagc tatacgggaa ccatttcctt
     7141 tatcaccgat aaagagaatg tgaagcaagc accgactagc tggaaggagc tgcaagaggg
     7201 tgactatcaa gttagcgtgg gggatgtgac gattgccaac caagcacaat ttgctgtatt
     7261 ggctgcggcg attgccaacg gaggtgacga aaaggatatt cagccaggtc tgaattacta
     7321 caaaaaaatg gcagaaaaag gccgtttgtc caccgttgat gccagcgtag caaatcttga
     7381 aaaaggtgaa atcgatgtgg cagttgtttg ggacttcaat ggactaaatt atcgtgacca
     7441 aattgactcc gagcgttttg aggtaactgt gccaacagac ggctccatca ttagcgggta
     7501 tacaacctta atcaataaaa atgcccctca tcccaatgcg gcaaagctag cacgggaata
     7561 tattttatct gatgaagggc aaatcaattt ggcaaagggc tatgcgcgac ctattcgcaa
     7621 ggtagagctg cctaaagaag tagctgaaaa attactgccg gaggatgctt atgaagcggc
     7681 ggtgccagtt gaagacaatg aagcttggaa caagacctct gtagagcttc cagagctttg
     7741 gcaggcggag gtactgacct atgcaaagta gacttgacaa actgattgta gttatactag
     7801 acggctgtcg ctacgacaca gccgtcgccc agcttgggtt catgaatcat ttagttgagc
     7861 accagcaagc aagcttattg aaggtaaaaa gtgagctgcc cagcaattca cgtccgttat
     7921 acgaagtact gatgaccggc gttccagcct atgaaaatgg catctatagt aattaccatg
     7981 ctcagcgatc caaagagtgg tcgctgtttg aattagttaa ggaagctggc ggaaaaacag
     8041 gagccgccgc ttaccactgg tacagcgagt tgtacaatca agccccctat aatcgtttgg
     8101 ttgatcgcat ccagcatcag gaggagcgac tgattcagca tggcatcttt tatagtgagg
     8161 acacttaccc tgattcgcat ttatttgcgg atgcccatta tttagtgaat cagtatcagc
     8221 cagacttttt ggtcgtacat tcgatgaata ttgatgatgt cggtcacaaa tttaccgccg
     8281 attctcatga atatgcagca gcggttaatc aggcggacag attattggct atttaccttc
     8341 ctgaatggct gcaaaaaggt tatcaagtcg tggtgactgc ggatcacggg atggatgcgc
     8401 acggactgca tggcggcagc ttggctgcac atcgtgaagt tcctttttat ttgatttcaa
     8461 ataaacgcca aaagctgaag gaacaaactg taccacagct gctggcggct ccattgtttt
     8521 gctggttatt aggaattgca ccgtcagaga aaatgcagga cattcaagaa ttgatgaggg
     8581 gatgaaagag atgaaaaaac gcttggttct ggttcctgcc tcctttatct tctgtgcatt
     8641 tttactgctg ccgctgcttt ttatggttct gtgcagcttg cacagtgatt taactaacca
     8701 gtggacattg gccaattaca gtgatatttt cagcaacaaa tattacacac aggcctttgt
     8761 taacagcgta ctgattgctg tcagctccag tgtgattggg ttaatcggga cgtttatttt
     8821 atgtttatgc ttaatgaatc tatcgactaa attgcaggag aaaatcacct ttgtttccaa
     8881 tttggcagct aattttgctg ggattccgct ggcattctct tttattattt tgttggggaa
     8941 tgtaggtgtg ctgaaaatga ttttgccatt tctagcggac ttcaatttgt attcttggtg
     9001 gggattgacc atcacgtata cctatttcca aattccgtta ggggtcttgt ttttgtatcc
     9061 cagcatgaag gaagtgaaaa aggaatggct ggagtccgca caattgctgg gcgcttcatc
     9121 agcgtatgct tggttcaagg tggctttgcc ttttttgcga ccatcgattg ccagcacctt
     9181 tgtcatctta tttgctaatg gcatggggac ctatgaaaca gcgtatgccc tgaccagcag
     9241 taatgtaaat cttttaacga ttcgaattgc ggctttagtg tcgggagatg tgttcgccaa
     9301 gcctaattta ggcagtgcgt tggcagtcgc tttaggtgtc ctgctggtaa cggcaatgtt
     9361 gattattcaa aagaaaggca aggtggtgca gacgtgaaaa agttaataaa accgattttg
     9421 ttctgctttg tcctgtatta cttgctgccg attattggaa cgattttgta tgccagctcc
     9481 actaaatggt caaaatcgct tttacctagt gattttacct tgcaatggtt ccaacagctg
     9541 ctgacggaca gggaatttat tgctgcggtg ggtcgctcac ttttattagc aggtgtggtc
     9601 ttggtgacga ttctgttgtt gatgattccc accattattt ggattcattt gtatttccct
     9661 cgtttgaatc gttggctgga aaagctgctc ctgctgccgt atgctttacc aggagtgatt
     9721 ttggtaacgg ctttgcttcg aacgtatgct gaaactggta tcccaatgtt tgtagtgttg
     9781 gtaggtgcat tgtttattac ggcgttgccg attgtctatc ttagtcttaa caaccagatg
     9841 cgcttaatta atctgaagga attagtggat gcagcagaaa ccttgggcgc cccgatgtct
     9901 acgattatta tccaggtgct gtttccaaac atccgtatcg gtgtgaccct agtttctctg
     9961 atgatttttt cttcggtttt tggggagtac atgctgacga acctgttgat tggcggtcga
    10021 tttcagacgg tacgcatcta tatgctgcgg cggatgaacg aaaacggcca tttggctagc
    10081 gctgtgatgg ttttgtattt gctgtttatg gtgggaattg ctttaactac ctttatgctg
    10141 acttatcgac aaaagcaaca gctgtccctt aagcagacga gggaagagaa gcagtcgaaa
    10201 aagaagccct taaaggagtt gtgacggaaa atgtttttag aaatacatga tctcaatctg
    10261 ttttttgatg aaaaacagat tttagctatt gatgaaattc agattgaaaa gggtgagctg
    10321 gttactttac taggtcccag cgggtgcgga aagtctacgt tgctgcgctg tatcacggga
    10381 ttggaacaac caaagagcgg tgagattgtg ctggacaatg agaatattgc cgataagcct
    10441 acgaaggatc gcaatatcgg ctttgttttt caacagtatg cattgtttcc aactatgact
    10501 gtttttgaaa atgtagcctt tggtttgaag gtaaagaagc tgagtcctga ggtgattcag
    10561 gaaaaggttt ttgagatgct atcattggtg gatatgacgg agcaggcaga taaaaatgtc
    10621 cagtttcttt ctggcggtca acgacaacgg gtggcactgg ctcgctcatt ggtcactgaa
    10681 ccgaaagtcc tgctgctgga tgagccatta agtgctttgg atgcccgaat tagaaaacag
    10741 ctgcagcggg atttgcgcgc tatccagcaa tcgctaggga tgacgatgat ttttgtgact
    10801 catgaccaag aagaggcgat gcgaatcagt gatcggattt ttgtgatgga agctgggcgg
    10861 gtggctcagg tttccacctc gaaggaattg tatcagaatc ctcagagccg atttgttgcg
    10921 gaatttatcg gcaactataa tcgtttcgaa tggtatgagc tgaatcagta tacccacgag
    10981 ttttccgcta agggcaatcg ctgcatttat tatttaagac cggagctgat tcaatttgaa
    11041 ccggttcctc agggggttaa gattccggtt ctatggaagg aatcatttat tttaggcaac
    11101 attcaacggt atgtttttca aacgctggag ggcaaagaaa ttttagtgga tcggttgaac
    11161 gatcgggaag agaaaattag cgatgcctta tacattcagc tgaaagatat tctagcaatc
    11221 cctgtggaaa ttgatactca gcattttgca caacaaagct agtgtatagt cggcaaacat
    11281 ttctggctct ttgtcaaata ggactgatag agtgatataa gaacatctaa ggtagaattg
    11341 attttctgct ttagatgttt ttttatgttt aataagtgat cagatgattg attaagaaaa
    11401 ttctggttct catcctaacc cgtaagctat tcgtttcaat gctttgatgt gggtatgcag
    11461 attttctatt ttgccatttg agtaaggcaa ctctaaggct aaacgaatgc cggattcata
    11521 gtttataaac gcttttttta tatgatgcag ttctcttgga accgacttgg gcattgtttt
    11581 tacaagctga aagaagatgt ccgcttcttt gtcataaaaa gcttgaagaa tttcttgata
    11641 gtaagcataa gctattcgta gttcatcaga agtagaaagt agacgttcta cgacttcact
    11701 ttcggttaaa taggcccaac caaaattacg ccattttgtc catttatcat aaacaagcgt
    11761 gtcctgtcgt ttgatcagaa gtcgagataa agccttcact tgtcgatagt gtttggcttg
    11821 tattggatcg tttcggtcga agcttttcat gactcatagt caaatatcct taaaactgcg
    11881 aatgaggtgc ttgacgatgt gaaagcgatc cacaacgagt ttggcatgag gaaagcactc
    11941 ttttatcagc aaagcataag aggcgttcat atctgttacg agtattttta cgtttaaacg
    12001 tgcacgtcgt tcaaattgca taaaataaga aatgagatct ttcttcttac gtgaaggaag
    12061 aatatccagt aatttcccag tatcgccatc catacaagaa aaagaatagg gaccgacttg
    12121 atttttcaat gaacgaaatt catctaccaa tgaacagcga ggcaaccaat ttattttcac
    12181 gtttgtcatg gggagcagag aagcgagtgt tcgcgaaacg gttgaccaag aaacgccgca
    12241 atgtttagaa atcgtcgtca ttgcttgttt ttcagtcaat tcctccaata ttttataatg
    12301 tacttgtttg gcgataaaac agtttttttc aacaatgtag gtattcgccg taaaataggt
    12361 gtgacagcca cgacagaggt accgttgctt tgccaaagca agataagtcc gttgattcgc
    12421 acacttattg agaaggacca gcgatttttt ctttccgttc ttaacgatca gcccatgttg
    12481 attcacctgt ttacactgag gacagggctg aatcgtttta atgagcgtgg cagaaacaac
    12541 gatactgttg attgaatgta tttttttctt ctcgacatga gtgatttgaa tgtttccatc
    12601 tttgattcct agtaatagtt tgataagatg aagatcaggc atacagaacg ctcctttgat
    12661 tgtgttgtgg tgattcaatt ctaacggagc ttctgtatgt tttgtttttt tatgctcaaa
    12721 aggactgata gaatgattta ctatcagtcc tagaaattat agcgccacat ttctttcacc
    12781 tttgactttt cagacggcag tttttatgct gatactgtag agagagcagg acacagcttt
    12841 tgcttgtttt gggtaagggg gaaagaagat gaccaaagca cttatttttg attttgatgg
    12901 gacgatgatt gacagtgaat ggatgtttta cgagagtatt catcactatt tactgaagcc
    12961 tatttactgg agcagttcgg ctataaaatt ccttttgcag actatcaaca aggtgttggg
    13021 acggcagcag aactgtcctt ttatgaaggg ataagtcaag cggttgggag accagtggat
    13081 gctgaacatt ttgagcagcg gatcagcagc actggaaaat cggagtagtc tcaattctga
    13141 actgcgggag ctagactatt tttttgaagc acatccgatg attaaggaaa aaattgattc
    13201 ctttgtgacg atcgatgatc ttcaggtcgg caaaccagat ccagaaggtt atttgttgtg
    13261 cttgaaacgg ctgagggttg aacaggctgt tgtagttgag gatagtccag tcggtgccca
    13321 agcagcaccg catatgacag ttgatgtaca agcgttggat gcagattttt tcgcctttag
    13381 cgggcataaa atgtgcgggc cgacaggaat cggtgtatta tatggcaaac gtcaattgtt
    13441 agaacaaatg gagccagtcg aattcggcgg tgaaatgata gattttgtca atctttacga
    13501 tagttcatgg aaagagctgc catggaaatt tgaagctggt actccgaaca ttgcaggagc
    13561 aatcgattac ttgaatgaga ttggtatgga gaatattcat cgttatgagc aagaactggt
    13621 tgcctacgtt ttacctaaat tacaagcgat cggcggtatc accatttacg gaccgcaaga
    13681 tcctgaacat catactggcg tggttgcttt taatttggat ggcatccatc cccgtgatgt
    13741 ggcgactgct ctggatatgg aaggtgtcgc tgtacgttca ggacaccatt gtgcacaacg
    13801 gttcatcgac gatttctgtt tgactaattg gtcgtttaaa cagtcgatga taatgccgtt
    13861 tgagcggctc caatgtcgtg gaatctttga gtaagagttt ccaatatcgc ttgagccgac
    13921 ggtacttctt ctgattttca gattacgtga ttgattttcg tacagttcta tgagaatcgc
    13981 ctgcttcagt tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa
    14041 ggtctgccca caatcatagc atagaaaacg ggtacgtttt aattctaaaa tagtcaactt
    14101 ttctttgact ttcaacatct gagtacgtgt cgtataggaa ccgttgcgga taatacgaat
    14161 cgatccacag tgttcacaat gggaaggtga gtcatccaat ctccctttaa taaactgcgc
    14221 ttgaacgcct ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc
    14281 tgttaatccg agtaattttc tagtatgatt atccatagag cgactccttt ggtagggtga
    14341 tgttttggtc gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa
    14401 agtgttagta gaaaatcgcc ttcgattttc caacaacaca aaatattata gaagcctttt
    14461 ttctaaagga tttcaaggtg tctgtttgca tttattaaaa aaaagaaaaa gtattggcaa
    14521 cagaaggcgt caccaacact agatattata gagccataat atgctgtata atatctagtg
    14581 tataggctta agtggaggta tatatgaaaa tattaactgt tgaagatgat aatatgataa
    14641 gagagggaat aagtgaatat ctttcagaat ttggatatac tgttattcaa gctaaagatg
    14701 gaagagaagc cttgtcaaaa tttaatagcg atataaattt ggttatctta gacattcaga
    14761 taccttttat aaatggttta gaagtgttga aagaaattag aaagaaaagc aatttaccaa
    14821 ttctaatctt gactgcattt agtgatgaag aatataaaat tgatgcattt actaatttag
    14881 tagacggata tgtagaaaag ccattttcat tgccggtctt aaaagctaga atagattctt
    14941 taattaagaa gaattatgga catttagaga agtttgaata taaggatcta tcggttaatt
    15001 ttaatagcta tacagctaaa ataaatggtg aagaaataga tgtaaatgca aaagaactag
    15061 aagtattaaa atgtttattg gataataatg gacaagtgtt aacaagaatg caaattattg
    15121 attatgtatg gaaagatagc gaagaaactc cttatgatcg agtaatagat gtatatataa
    15181 aagaactta
//
LOCUS       DAHHZJ010000056        14911 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.057, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000056 DAHHZJ010000000
VERSION     DAHHZJ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.057"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="NBL02_001398"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="NBL02_001398"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCD2572089.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="NBL02_001399"
     CDS             complement(2260..2511)
                     /locus_tag="NBL02_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HCD2572090.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="NBL02_001400"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="NBL02_001400"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HCD2572091.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="NBL02_001401"
     CDS             3461..3679
                     /locus_tag="NBL02_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572092.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3968..4480)
                     /locus_tag="NBL02_001402"
     CDS             complement(3968..4480)
                     /locus_tag="NBL02_001402"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCD2572093.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="NBL02_001403"
     CDS             complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="NBL02_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCD2572094.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6933..7376)
                     /locus_tag="NBL02_001404"
     CDS             complement(6933..7376)
                     /locus_tag="NBL02_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HCD2572095.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7377..8165)
                     /locus_tag="NBL02_001405"
     CDS             complement(7377..8165)
                     /locus_tag="NBL02_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCD2572096.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8162..9103)
                     /locus_tag="NBL02_001406"
     CDS             complement(8162..9103)
                     /locus_tag="NBL02_001406"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HCD2572097.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9280..9867)
                     /locus_tag="NBL02_001407"
     CDS             complement(9280..9867)
                     /locus_tag="NBL02_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HCD2572098.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10088..11395)
                     /gene="obgE"
                     /locus_tag="NBL02_001408"
     CDS             complement(10088..11395)
                     /gene="obgE"
                     /locus_tag="NBL02_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HCD2572099.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11689..12699
                     /gene="gap"
                     /locus_tag="NBL02_001409"
     CDS             11689..12699
                     /gene="gap"
                     /locus_tag="NBL02_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCD2572100.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12784..13719)
                     /locus_tag="NBL02_001410"
     CDS             complement(12784..13719)
                     /locus_tag="NBL02_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD2572101.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            13905..14864
                     /locus_tag="NBL02_001411"
     CDS             13905..14864
                     /locus_tag="NBL02_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HCD2572102.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT         4219 a         3291 c         2625 g         4776 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa
     3721 cttttacggg ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta
     3781 aagccagttt ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg
     3841 gtctttgctt gtttccattt ataaaaaaat agcgaccctc aaaagtcaga tagagaatct
     3901 gacttttgag ggtcgcttta aaatcgcttc tttaagaata acatcaagaa tagcttcttt
     3961 tttctgctta atattccatt aatgtaatga tgtcatatcc tttgattttg tcgcgtccat
     4021 ggagatccat caattcgatc aaaaatgcgc agcctacgac tacaccgcct aattgttcga
     4081 tcagttcgat cgtcgctttg attgttcctc cagtagctag caaatcgtca caaatcagca
     4141 cacgttgacc tggtttaatg gcatccttat gcaatgtcaa tgtatctgtt ccatattcta
     4201 atccataagt cacttcgatg gtttctcgag gtaacttccc ttttttgcgg actggggcaa
     4261 aaccgactcc taactcataa gcaacaggac atcctacgat gaatccacgg gcttctggcc
     4321 caactaccat atcaattcgt ttttcttttg cataatttac gatttgttta gtagcttccc
     4381 gataagcgtc accatctgcc atcagtgggg aaatatctcg aaacacaatt ccttttgacg
     4441 gatagtcggg aatactggca atataatctt ttaaatccac tctttgtttc ctcctaggtt
     4501 gttaaccatt tctttatggt aggaatatcg ctcaaaagca aaaattcctc aactttgatt
     4561 ttattgagcc gtcgctggta taatctgctc tcagtcaaag gatgactttc tgggctagcg
     4621 actttttgta aaacaccgtt ctctattgta acaaatttca attcaaaaaa cacctgtatc
     4681 ataaaaatta ataatttttc agggatacgc aaatattgag ctattgcttt tattttatga
     4741 cgtatatcaa ttcgttcttg agaatggatc aatttaaaca attttgcgta ctgttctctt
     4801 gtgcctaccc cattcaaata ggcctcatca ggtgaaatcc ccatcagata aacacgattg
     4861 aatgaataag accgaaaaac ttctttcaga tccgctaaat catctggaca atccataaca
     4921 actagagagt ttggttttct tttttcaatt attgccttta cagaatctag tccttcgaaa
     4981 acaatcgcag cttcctggat aatctcaggc acgaatttca acgtcgaaga atcaaatgct
     5041 aaataaaggc taccttccga cgttcggctc tgttcacgga aacgttttgc ccgccaatca
     5101 aatacttgca gaccacccac agaaaaatca gataccatca gctgcggttt cttacgcccg
     5161 ttccattcat tgatcgaaag ctttcctacc acatctacct gattattcag cagttcttct
     5221 tcttgagtac caaaaccaaa agcgaccgca tcaagctgac tcgatgcatc ggaaaggaca
     5281 aatttcagat gctgttggtt tgctccgatt cttttgatat tttctgcttg gacttgtcga
     5341 aataaaaact gaggcaaagg attatccgta ccaaatggcg ccaatagttt cagctcatca
     5401 attcgctcta ctgttacttc atttggcagc aatacttcat caattctcaa ctcaggccct
     5461 ctcatcaagt caatttgatg atcgactata tattggttca tcttctcctg tagtatagtg
     5521 acattttcac ttggcatcgt taaaccgact gctgcatgat gtcctccgaa gtaagtaaat
     5581 aaatctcgca tctggtcaag catttcaaaa agattcaagg cttcaatact acgtccagat
     5641 cctttagctg atccatcttc ttttaaagta aggacaagcg ttggtttacc cgtttcattc
     5701 attattttcc cagcaacaat gcccaaaacg ccttcatgcc atcctcggtt taccaataga
     5761 tggatatgat tctccttatt gaccatcgct aatgcttctt gtgttatctg ctctacgatt
     5821 gcttttcttt cttcattgat ctcattcaat ttttttgctt gaacggatgc ttcctcttca
     5881 tcaaacgtcg caagtaacga gacggcagga cgtggatcgc ccatccgacc gattgcattt
     5941 agtctgggag caatcgaaaa accgatcgtt gtttcgtctg cgtccctcat ttttacgccg
     6001 ctttcttcaa atagcgcctg aagtcctatc cgttctgaat gatgaatggc atcgattcct
     6061 aatgaaacaa ggattcgatt ctcatcagtc aatgaaacta aatcggcgat cgttcctatc
     6121 gctactaaat ccaaaaattc tgcaggcggt tcttccaata atgcagaagc tactttgaat
     6181 gctactccga ctcctgccaa atctggaaat ggatagtgcc ccgctggatg acgagggtga
     6241 acaatcgcat aagcgtctgg taaattctca ggtagttcgt gatgatctgt aatgatcacg
     6301 tctacaccag cttgctgagc gtatgctacc gcatcatttc ctgctacccc gttatcaaca
     6361 gtgatgatca actggattcc ttcttcgatc ttttcttggt ataccgattg gtttggtcca
     6421 tacccatgct caaatcgatt tggcaggaat acttctactt ctgcaccaag taattccagt
     6481 gtttctttca ttaccgttgc actggtgatc ccatctgcgt cataatcacc gtaaacgagg
     6541 attttttctc cactgattac agcttcttgg attcggctga ttgctttatc catatcgtgt
     6601 atcagatacg gatcatgtaa ttcttgttcc tttggatgta aaaatcggtt gatatccttt
     6661 tcctcatggt aacctctttg ccacagtaac ttaccgacaa atggagataa tttcagttca
     6721 cggatcattt ggagaaagga ttcagaaggc tctgtctcgc ttaacttcca atcaaaattt
     6781 gcttgtctca ctttctcact cctaacttag ttattatagc aaaggaaatc agcaaaaaaa
     6841 agcagaaaga aagaataaaa acagatcaga tccgctagac agatgttcag caggacctga
     6901 tcttttgtac gaccatttat gaaatatccc tattagtcaa aataattgaa gctttgtttg
     6961 ggcgattgag ttttttgttc ttccggcgct ttcaattgac tgatttgtgc ggttttcgct
     7021 tgtaactccg cctgataagc agcttccaac tccgcacgtt cgtttctaaa ctcgcgttgc
     7081 tgttcttctt ttgcttcttc aattttcttt tgaagttctg tttgatattc tgacatttcc
     7141 tgagtcagct gtttgatttg ctttttttgc tgccatagtg ttgccgaagc agtcaaaaat
     7201 acaatcagtg caccaatcag cgcagaaccg atgatcacta agatcaatgg cgcagaaaag
     7261 gaagtgaagc caaaacttac aggaacattt ttattgttca atacagcaaa aagaacaata
     7321 atcaaaacta aaacaaatcc aagaatcaca ttgctttgct ttttcaattc cttcacctac
     7381 tttttgttaa aaattccgct ggctaaataa tcaccaatcg ttggaaacag cgtataaaat
     7441 tttgatgcaa cttctaaaat aaacggttga ttgatttccc gttttggatg tctcatattg
     7501 cggacgatca tttttgccaa cttggcaggt tctaaaacga tttgtccgac tttttccaaa
     7561 tagcttccgc ttggatcagc cttatcgaag aaatttgtct cgattggacc tggattcact
     7621 gtcgtaacag ctacgccaag tggtttcaat tctaatcgca aagcgttgga gaagccaagg
     7681 actgcaaatt tcgtggcaga ataaaccgtt gattttgcag ttgccatttt tccagccata
     7741 gaggcgatat taatgatatg accttgtctt ctttctgcca tatcgatcgc aaatttttgt
     7801 gtcaaaacca tcattcctaa gatgttgact tcaaacatat cgtatgcttt tccaaggtcg
     7861 aatgtaagga agtcttcgaa aatcccaaaa cccgcattgt ttaccagaat atcgacgcgc
     7921 ccgacttctt ccatcacctt ttcatataat tcgtccacgc tttcaggatt gctgacgtct
     7981 accttgaaag cataagcctc tttgccgctc aagcgtctgc actcgtctct gacaccttct
     8041 acaaatgcca ttcttcgtgc gcaacttata acgattgctc cttgctttgc tgcttcataa
     8101 cagatttgtg cgcccagacc gcctgaacta cctgttacta aaacaacttt atcttctaaa
     8161 ttcatgcctc ttcctcctcg cgaaatggaa tctcaatgct atcaaaatct ttcataatcg
     8221 tcgtgttttc aaacacttct tgtgcttctt tttcaagctc taaagctgct tttcctaaat
     8281 atctcgcact gatatgagta agtagcagcc gttttgcctg cgcttctttt gcaacttcag
     8341 cagcttgatg agtcgtggaa tggaaatagt tgtgcgccat tcttgcttca tctttattaa
     8401 atgtgctttc atgtaccaga atatcgctat tttccgcaag cacaacggaa ttatgtgttt
     8461 ttctcgtatc gcctaaaata gtcacgattc gtcctttttt atctggacct acgaaatctt
     8521 ttccattgat cgtccgccca tcttctagtt ggatcgtttc accttgcttc agtttgccgt
     8581 acagtggtcc agctggaata cctaattctt ttagacgatc cacctgcaat tctcctttat
     8641 gatcatgttc aaccacacga taaccaaaac tgtcgatccc atgatttagt ttttttgtat
     8701 aaacagaaaa ttgctgatca gtaaaaatcg gggctgtttc atttagctca atgaatttca
     8761 gcggataact taaacgcgtt tgcgaaatac ctaaagagac ttttatgtat tcctctatcc
     8821 cttttggccc gtaaatctct aatggcgtat ccccaccttg aaatgaccgg ctgctgatga
     8881 gcccgggaag tccaaaaata tgatcgccgt gtaaatgtgt aatgaaaatt ttaccgattt
     8941 ttctcggacg aatagtcgtt cgcaaaatct gcatttgcgt gccttcgcca cagtcgaaca
     9001 accaaacttc attgcgctcg tctaaaagtt tcaatgctat acttgtcaca tttctatgtt
     9061 ttgcaggaac acctgcacct gttcctaaaa attgtaattc cataatctac ctgactttct
     9121 ttaagttcct ctttaatttt agggtaaaaa gaagggataa tcaaccgtaa gtcgagaatg
     9181 ggttcaaatt aaaaataacg ttctaaagcg atagcgtttt ctttgaaaat tactataaaa
     9241 acagcagcga acccgtttga gatcctctgc tgttttttat taatttgaag atccagaact
     9301 ttcattgtcg ttggaagttt gcggatgttt gtccttccaa gtcttttgtg cagcttcttt
     9361 cgcatgattt tcagcttctt tcgcatcatg cacatctttt tcgacatctt cataatccaa
     9421 gcgagtaatt tctccgccta attccatcat taccaattga ttcagtgggc gattgtcctc
     9481 ttcatcagca atcaagatca gtcctgtagc tccttcaccg attttatttc ctacaaagtc
     9541 gaacacggtc tgggcatctt ttatttcttt ggcatcttgt gtcgcaccga tcattcctcc
     9601 ggcaaaccat cctaataaaa tccctagcgg accacctaat acaccaacca acattccgat
     9661 taatccaccc gtagaagatt tgttattacc ggtaaagtcg ataaaatctt ctattttgaa
     9721 ttgatgtgtc ccattaggat catggctgac aactgccatc tgttctcctt taatcgaacg
     9781 aacagcatgt aactttttga tttctgaaaa tgcctgatat gcctggcttt ctacttgaaa
     9841 atttaaaatg attactcgtt tgctcattaa aaacgcccct ttcatacctt cttgttattt
     9901 attttacctt taattgaaaa ataagaaaag aaaagcactc tttttttcca ataaaatagg
     9961 gctgtgacaa aagtcgtatc acagctcctt tttccgaata aacggcgttc ttgaagctga
    10021 tcacttctgt cacaacctcc cttaaatctg aatctatatt ttttggtatt tgtttttatt
    10081 tgcttgccta ttcgacaaat tcaaattcaa actcaccgat acgtacaagg tcgccatctt
    10141 tcgcaccgcg tgcccgaaga gcttcatcca cacccatacc acgtaattga cgagcaaaac
    10201 gcatcactgt ttcatcatga tcgaaattcg tcatttggaa taatttttcg atcttctcac
    10261 cagataagat ccacgttgca tctgaatcac gatcgatttg gaactcagga ccttctggat
    10321 tgaatccata gtgtacagtt tcttcttcca gttcttcttc gtacaatggg aattctggcg
    10381 ttacttctaa caaatctgct gtagcattca ataacgcgtc caatccttgt cgagtcactc
    10441 cagaaattgg aaatactggg atgtcatcag caaattcatc ttctttttcc ttgttcagct
    10501 gttctttgaa tttcactaga ttttccggtg catccggcat atccattttg tttgccacga
    10561 tgatttgcgg acgctctagc aagcgtaagt tataagtaga taattctttg ttgatggcaa
    10621 gatagtcttc ataaggatct cgtccttcca tcccactcat atcgatcaca tgtaaaatta
    10681 ctcgtgttct ttcgatatga cgaaggaatt gtgtacccag cccaacacct tgcgaagctc
    10741 cttcaatcaa gcctggcaag tctgctgcgg caaaacttct gccatcactt gtagtaacca
    10801 ttcctagatt aggtacgagc gtcgtgaaat gataagcgcc gatttttgga cgagcagatg
    10861 aaataacgga tagtaaagtc gatttcccga cagatggaaa acctacaagt ccaacgtctg
    10921 ccaatacttt caattctagt tcgatttttc gctcttgtcc tggttcaccg ttttcagcaa
    10981 tttccggtgc aggattccga ggagaggcaa aacggatatt ccctcgtcca ccgcgtccac
    11041 cttttgccac aactagtgtt tgaccatttt caattaaatc tcctaaaaga gcaccagtct
    11101 ccgcatctcg gacagtggtt ccttgtggta ctttaacata ggtatcttct gaaccacgac
    11161 cgtgcatccc tttactcatc ccattttcac ctggctgcgc tttgaaatgg cgattgaacc
    11221 gaaaatccat caatgtacgt aacccttcat cgacgatcaa gatgacatcg ccgccacgac
    11281 caccgtctcc gccagccggt ccgccgtcag gaacgtattt ttcgcgacga aatgcaacca
    11341 ttccgtctcc gcccttgcca gctttcacat cgatcgttac ttgatctaaa aacatggaca
    11401 taattaagtc ctcctattat tcatttattt atttgactgc ctgaatttcc acaataaaca
    11461 aaagtcttac ttattttatc ggtgaaatct gattttgtct atcatttcag caaaaagatt
    11521 gtttaccttc ttcttttttt atcatttggt aaggtttcca accttttttc tgctaccaag
    11581 tataattttt tgacaaaata ttctttgcgt agcagaataa cagtgtatta attgtattaa
    11641 ttgaggatgc cgtttcacag cacccctaat aaaggaggaa cttttgatat gacagtaaaa
    11701 gtaggtatta acggatttgg gcgtattggc cgtctggctt tccgccgcat caaagaagtc
    11761 tcagatgata ttgaagtagt agcaatcaac gacttaacaa gtccaactat gttagcacaa
    11821 ttattgcaat ttgactctac tcatggtact tatcctggca cagttactgc aacagaagac
    11881 tcaatcgtag tggatggcga agcaactcgt gtttatgcag aacccgacgc aagcaaaatc
    11941 ccttgggcaa aagaaaacgg tgtagatatc gtactagaat gtactggttt ttatacatct
    12001 gaagaaaaag caaaagcgca tttagatgct ggtgtcaaac gagtagttat ttctgcccca
    12061 gctggtgcta tgaaaaccat cgtctataac gtcaatgatg atacattaga tgaaaacgac
    12121 aaaatcatct cagcaggttc atgtacaaca aactgtttag cacctatggc ttatttctta
    12181 aataatgaat ttgggatcga agttggtaca atgacaacag ttcatgccta cacttccaca
    12241 caaatgctct tagatggacc tgtaaaaggc ggtaatttgc gtgctgctcg ttcagctgct
    12301 gacaatacta tcccacattc tactggtgct gcgaaagcaa tcggcttagt tatccctgag
    12361 ctacaaggaa aattacaagg acacgcacaa cgtgttccag tcgttgatgg ttcattaact
    12421 gaattggttt cagtattgaa aacaaaagta actgctgatc aagtaaacga agcaatcaaa
    12481 aaacacacag ttgacaatcc ttcattcggg tatgatgacc gtcaaatcgt ttctagcgat
    12541 gtcattggaa caactcaagg ttcgatcttt gacccaacgc aaacagaagt aacaactgct
    12601 ggtgatttcc aacttgttaa aactgttgca tggtatgaca atgagtatgg atttacttgc
    12661 caaatgattc gcttactaga aaaattcgct aacttgtaat ttgatagatc aatcgataaa
    12721 agaagccttt gactggatcc agtcaaaggc ttctttcttt tcatccccta cttttaaact
    12781 cgttcaatga acatattggc ttaaaaattg ttggactact tgctgatatt tttcaggatc
    12841 tgtcttgtat gcttccgcat gctcggctcc ttttacaaca tacttttctt ttggtccctt
    12901 cgttgcacga tagaccttat ctaacatttc aaatggtaca aatgtatctt cgtctccatg
    12961 tataaagagc attgggcgct ggtttttctt caattgttcc actgcatttg cttcaccgaa
    13021 aaagtagcca gcacggactt ttgtcactag actcgttact tgcataagcg gaaatgctgg
    13081 aagactaaac atatcgtcta gctgataagc aagctcatca tgaacggaag aataaccgca
    13141 atcttcaatg attgctttca cattatctgg tagtttttct ccgctagtca tcataactgt
    13201 tgctgctccc atgctgacac cgtatagaac gatttcttgc gattttccat tgttttctag
    13261 cactttgttt atccactgta catagtcttt acgctctggc catccaaatc caatgtaatc
    13321 cccttcgctt tttccatgtc ctcttgcatc aggaacaagg acattgtaac ccatatcatg
    13381 aaacatcttt gcgtaattgg tcatcgtttc agcgtttccc atatagccat gagccacaag
    13441 agctgttttt tcacttttcg tttcagcagg aagataaatc gctgataatt tcagaccgtc
    13501 ttttgaagtg atggtccaat cgcttcggtt ttcttttgaa ctaaaccatt tatgtgcatc
    13561 cgtttctttg gatgtctgtg ttgtaccagg tgtatcttcg tctaaaaagt ctttctcaga
    13621 aggcacaaca gcataattat aaaaataatt ccctgcaaac actaacgcaa caatcagagc
    13681 taaagcgacg cctaaaagga ttttcatcca aagtttcata aatcatccat ctcatttctc
    13741 ttttttcata taaccttgaa ttagtataat caaagaatct ctactatgca atgttcattt
    13801 ttataacccg cgaattgtaa aattaagtta gaaaaataaa aaggcattta tggtacactc
    13861 aaattgtatt tccgacgaaa gaaaacaaag gagtgtaacc ataaatgacc tacaaacatc
    13921 ttaccataga cgaactgaca atgatagaat catattatct tcaacataat aagccggttg
    13981 aaatcgctaa ccgaatggga cgtgctatac aaactattta taatgtagtc aataagttca
    14041 agcaaggcaa gactgctctt gattattggc accagtataa agaaaataag aaaaaatgtg
    14101 gtagaaaagt cattcaatta cctgctcatg aagtagatta cattaaagag aaagtcactc
    14161 ttggttggac gcctgacgtc attatcgggc gaaaagaaag gtctgtttca tgcggtatga
    14221 gaacacttta tcgtttattt tctaaaggaa tatttgatat tgacacacta ccgatgaaag
    14281 gtaaaagaaa acccaatggc catcaggaaa aacggggaaa acaacagtat cagcgctcaa
    14341 tccatgatcg acctgataat tatcctgatt tcaattctga gtttggtcac cttgaaggtg
    14401 atacgatcgt tggcattcat cataaaagtg ccgtcattac tttagttgaa agattatcta
    14461 aagtcattat cacgattaaa cccaacggcc gtaaggcatt agatattgaa actgccctta
    14521 atcaatggtt ttctcgcttc cctaaaaact tctttaaatc tattacgttt gactgtggaa
    14581 aagaattttc taactggaaa gccattagta accaacatga tattgatata tattttgcgg
    14641 accctggaac accttctcaa cgcccattaa acgagaattc taacgggatt ctgcgtcgta
    14701 atggactgcc gaaatcaatg gattttagaa aagtgaatca gacatttatt tccagtgtca
    14761 gcaatcaacg taatcatatt ccaagaaaat cattgaatta cagaacacca atcgaaatat
    14821 ttttgagcta tgtacaagaa gcattttatt ctagcttaat ttgacaaatc ataacctata
    14881 aaaaaacaaa ataaaaaagc agaaataact g
//
LOCUS       DAHHZJ010000057        14636 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.058, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000057 DAHHZJ010000000
VERSION     DAHHZJ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.058"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            19..294
                     /locus_tag="NBL02_001412"
     CDS             19..294
                     /locus_tag="NBL02_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572103.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            336..758
                     /locus_tag="NBL02_001413"
     CDS             336..758
                     /locus_tag="NBL02_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572104.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            777..2369
                     /locus_tag="NBL02_001414"
     CDS             777..2369
                     /locus_tag="NBL02_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572105.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            2381..2716
                     /locus_tag="NBL02_001415"
     CDS             2381..2716
                     /locus_tag="NBL02_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572106.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            2863..3057
                     /locus_tag="NBL02_001416"
     CDS             2863..3057
                     /locus_tag="NBL02_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572107.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            3089..5461
                     /locus_tag="NBL02_001417"
     CDS             3089..5461
                     /locus_tag="NBL02_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572108.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSSEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            5522..6982
                     /locus_tag="NBL02_001418"
     CDS             5522..6982
                     /locus_tag="NBL02_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCD2572109.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFIPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            6993..9197
                     /gene="topB"
                     /locus_tag="NBL02_001419"
     CDS             6993..9197
                     /gene="topB"
                     /locus_tag="NBL02_001419"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCD2572110.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAESIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ENDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            9213..9362
                     /locus_tag="NBL02_001420"
     CDS             9213..9362
                     /locus_tag="NBL02_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572111.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            9436..10029
                     /locus_tag="NBL02_001421"
     CDS             9436..10029
                     /locus_tag="NBL02_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HCD2572112.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            10045..10944
                     /locus_tag="NBL02_001422"
     CDS             10045..10944
                     /locus_tag="NBL02_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HCD2572113.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            10947..11432
                     /gene="ssb"
                     /locus_tag="NBL02_001423"
     CDS             10947..11432
                     /gene="ssb"
                     /locus_tag="NBL02_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCD2572114.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            11798..12058
                     /locus_tag="NBL02_001424"
     CDS             11798..12058
                     /locus_tag="NBL02_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572115.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            12499..12705
                     /locus_tag="NBL02_001425"
     CDS             12499..12705
                     /locus_tag="NBL02_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HCD2572116.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            12705..12956
                     /locus_tag="NBL02_001426"
     CDS             12705..12956
                     /locus_tag="NBL02_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572117.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            12971..13408
                     /locus_tag="NBL02_001427"
     CDS             12971..13408
                     /locus_tag="NBL02_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572118.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFESIEKNGAFYTIESEEKDNE"
     gene            13401..14108
                     /locus_tag="NBL02_001428"
     CDS             13401..14108
                     /locus_tag="NBL02_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572119.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGK"
     gene            14489..>14636
                     /locus_tag="NBL02_001429"
     CDS             14489..>14636
                     /locus_tag="NBL02_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572120.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEK"
BASE COUNT         5571 a         2390 c         3013 g         3662 t
ORIGIN      
        1 ataaatttgg aggaaaatat ggacttatta attagaaata ttgaagaaaa gtatattaat
       61 aaaattgaca aacgttgtga agaacttacg gtcaagactg gcaaaaaatg gagtcgaaat
      121 caatacctaa aagttttgat cgaaaatgat tttgatcacg ctcttctgaa ttataaaaaa
      181 gaccaatttg atcggttgtt agaaaaattt gtggatattc aaacttataa tacgaaaact
      241 ttagaagaat atattgcaac aaataatcaa ttaattggtt tgttaattga ataataaaaa
      301 tagtattgtg aatttataaa aggaggaaat caattttggc aaaagcaaca agaaaacatg
      361 tatatcttta cgatatgcat atcgaagtac ttgatgaaat aatttctact catgaagata
      421 tatctaatta tagtgaagca gtcagacatt tgtgtatgat gtatgatcca aaaatcgaag
      481 aaaaaataaa tccaattcaa attaaattga atgcaatggg aaaagaaatt tctattttaa
      541 ccgaaatggt ttcagagttt gcggatcttc atttgaaaga tacaggaata ctaattggta
      601 caaattcaac tgtttatcaa gaagcagaag aacgtgtgga aggaaaaatt aaatccaatc
      661 aaaccaaaaa atattcttct aagttaaaga aaccagtgaa tgaatctgtt cataccagag
      721 tagatccatt tgatccaacg aagattctaa aaagataaaa gtagaaggag aacgtgatga
      781 ataaatcgcc tgcagttatt cttacttgtc aatttacctt gcccaataaa aaatcttttt
      841 caacatacat tgattatatg acaagagaaa aagcactgga agaaatggaa cgacgtacgc
      901 ccgaagaaga acaggaactg caacgaatca agaatgcttt agaggatttt tatattcctg
      961 aaggagaaac tttttcggaa agtaagacgg ataaagaatt cagtgaaacg tcgaatgaag
     1021 ctcaatcgtt gatggaacat ggaatcaatt ttgacaagtt agaggaacaa gattttacca
     1081 aatatctttc ttatatgacc cgtcactatg cgttagaaca aaagaaagaa ctgacgcaaa
     1141 gggaacaaaa agaataccaa cggttgaacc aagcaatgaa aaaatatcaa acagagccac
     1201 ataaaaaagc ttcaggaaat cttcctggag ttttttctat ggcttcagac gaagtgaaag
     1261 gaagtgatct gaaggagatc aaacaaatct tccaaagagg acaacaaaaa ggctcaatca
     1321 tttatcaaga tgttatctct cacgacaatt cgtatcttga aaaaatgggc ttgtacgatc
     1381 ccaaaacaga taccttagat gaagaaggtt tgaaagcagc tggaagaaaa atgatggaga
     1441 cattattcga gaaagaacaa ttgaatgaaa caggttactg gatggcaacg attcaccgta
     1501 acactaagca tatccatatt cattttgcag ttgttgaaaa agaaaatacg cgtaaaccgt
     1561 atactgagat tgaaaatggt gtgagttata tcgtaccgaa ggggaagaga agacaagcaa
     1621 cattagacgc aatgaaaacc aattatgtac atgagttaga acgtttctct tatgaaaagg
     1681 atcgaatgat cggtatggag aaacgaaatt tattgacacg aaaaagtgat ttgcgtaaca
     1741 ccttaacaaa agtagtaaaa gaaccagcac gttacgatcg acacgccatg aacttattaa
     1801 atgaagtata taaaacccta cctgaaaaac gttcggaatg gaattatgga gatgaaaatc
     1861 gaaccaagct ttcaccattt acaaggggaa aacttgatga gttaaccaaa tatgttttaa
     1921 caaaagaacc tggatatcaa gaatatatac agatttcaaa agcattgaaa gaagaagcca
     1981 aaagccttta tggggaaagc aaaagagaaa caaagaatgc agaaaaaaac gccctctttg
     2041 atttaaaaaa gcgcacagga aatgcgattt taacagaact gaagagacag gatagtcaaa
     2101 taaaaccatt aatagaaagt gtcaatcaga tgcaatctat ttacaatgaa cgatcctttc
     2161 gaacactcaa acagttgcca acaaatgaat acaagtatag ttatcaagaa ttcgtaaagc
     2221 gtaggaaaca attccaacaa agagttgtca cgcagcgaag cttgcgtcaa ttaaatcgaa
     2281 caattaatgc acatgaagaa aaataccacg caagtaaagc atatgaagag caacagcgaa
     2341 gaatccaaat ggaacaagaa agacaatagg aggaagagaa atgaaacaat atttagatca
     2401 atggaaagtc atagaaggat cactaaaaga agaacgaatc gaacaacttc ctgattgttt
     2461 agaaaaagag catttgtttc agataagcga aatgttgaga aacgaacagt ttgatccaaa
     2521 tcaattttta gttgttgagt acccagctac tggtgtgtat tgttgtaatc atgtgaaggg
     2581 tgaaaaatat tttattattc aagaatacga aggtaagtta gcgccttatt atactacttg
     2641 ggaaataaat gaagagggaa tcaataattt cccatgtgaa agcattgaag agtctatttc
     2701 tctcacggaa tgttaaataa atagagacgt aaaagagtcc tgaacgtgat tgtccaggac
     2761 tctttttcaa tgaattgttt tccgccaatt cactgagtgg tgcgattcaa ttttattata
     2821 tcaaaagaaa gtgagattcc aaagtgaagg gaggaagaag aaatgactca gacaacgatt
     2881 ggtgttgtgg ttgaaaaaag cagggataat ctcgtttttg tgacagaaat tcaaacaggt
     2941 agagcttttg tagtcacaga caaagctgca aaagcgtatc aaaatggcaa tattttaacg
     3001 ttaaatctga ctacgaagat gttcgttgat gcagtagaaa attatccatt tgtttagaca
     3061 aacgatattt tacaaggagg aaacggacat ggcagaagaa aaagaaccac tagataatag
     3121 tcggttggga aagtccaaaa gaaaattagt acggttgcaa aacgaattaa ataatcaatt
     3181 tcaaaagcta tttgatcacc aaaaattgac aaatgggcaa ccaatgaatg acaaacgaaa
     3241 tggtcgttca tggtttaacc agcaagaaaa aatagaaaat aaaatacaaa gtttgagaga
     3301 agagatcaag caacaagaaa aacaggtaga aaaacttgaa agacaagaag agctaaagga
     3361 aatgggctat aacaagtacg gcggactgga tatgacgatc gataatattc caagaatcaa
     3421 agaagaaatc gaacgctttg aaaaaggtga atcgactttt tcagccgcca cgatccgaaa
     3481 atatcaacgg aagttggaaa cattagaaca gctgaaagaa aaatcagaaa aaggaaaaga
     3541 aaatatcctc cctgaagtac aagcgatcat tgacagcgga cgagtgacgc aatggaaaaa
     3601 aaatccaacc atttattttt tgaaaggcta tcgaaaagtt gctttagaac tgaatgaaaa
     3661 cgggggattt gaggaatcaa aaaaatacca agccacgtcg gaagaggaaa aagcagctgt
     3721 tcgtgaatta cttgaagagg ctgcaccaac tgattctctg aaggagaaag aaaaagatca
     3781 tttctatcga gtagaattca acgaaaatga tccagaaaaa ggattgaaaa gttatggtgg
     3841 ggagatcgta acattagaat tgatcgatca acttaaaacg ttggataacc aatacgctgc
     3901 agaagcaggt tattataaat tctattttga agaagtgaag aacggagaag tagtagatca
     3961 gatccgaatg gatctagggg atggcgtcaa cagtaatcac cccatctatt tagagttagc
     4021 aaaattgtgc gaacaagtac ctgagaagga acaaaaacaa tcggtcaaaa atgaacctgt
     4081 ttcgaaagaa caaatgcagt cgaaagaaac aaaaacaaaa caaaagggct atcaaaaacg
     4141 aacggcagaa gagatcaaac aagaagttaa atccctttcg caacaggcac tggatgcggt
     4201 gaaaaagcat acccattctt ctgaagacgt caaagaactt ttgaacttta tgtctcgttt
     4261 tccagaacga tcctttagaa atcaattatt gattgaggaa caatttccag gtgcaacggc
     4321 ttgtttaggt cgtgccgcgt tgaaaaaaga aggaatctat atcaaaaaag gggaacaagg
     4381 gaacaagatc tttgtcagaa aaacggtcaa aggattttat gagaaaggac gtggctttgt
     4441 tcgagaaaca gaagctacac cagaagataa aaaacgaatc gaaagtggtc aaatcgaagt
     4501 gatcaaaaaa ccttactaca cgattgaaaa agtgtttgat atcacacaaa cacagttaaa
     4561 acctgaagac tatccgaaaa tctttccgaa ccgtgtcttt gattttcaat tggataaaaa
     4621 aggacagtca gaacttcaag ctgggattca agcggtagcc aacaatattg gaatacaaat
     4681 tcgagatatg acagaaagtg aagtctatca aagagaatta ggacaagcac gtggggcgta
     4741 tgttcgtaac gaatttactg gcgaagaaga aatcgtcatg aatactcgaa atactcctac
     4801 gcagcattta gctacaagta ttcatgaatt agcccatgca agaatgcaca aattttcaga
     4861 attagatacc gcaactaaag aattgcaggc agaaatgact tcttatattg tgtgcaaaca
     4921 ttttggaatg gacacgtcgg agaaagctat tccttatatt gcagcatgga cgaagaatgg
     4981 tcagacgttg gatgataagg aagcggcgga acgtgggaaa atcatgaatg atgtcagccg
     5041 tgtcgcaaat gaattcattc aaaccatttc taatgaaatc aatcaataca gagaaagaga
     5101 accagagatc caaccagcag aagtgaagca ggagcataaa aatactgtag aaacggcaga
     5161 gaataagaat attgtgcccg ttggttcact ttggatcgac aagaaagacg ggaaagtaat
     5221 ggaagaagat acgggtcgaa cgttacattt gaaggatgcg gcgtttattg aagatccgca
     5281 gcataatcaa gtgattttaa aaggaaaaag ttatggaaaa gagattcaaa cggcgttacc
     5341 ttcagaagaa tttgcgctag gaaatccatt ccgattttca cttgaaacag tgtcagaagt
     5401 ggccaaacga aaaaatgaaa tctattatga acaacaattg tcacaaaaag aacgcgtata
     5461 aaacaagtaa aaaagactat ctgaaaaacg gatagtcttt tttcttttgg gaggtttgaa
     5521 aatgaccgaa aaaaaaatga gtcttattga tcgatgtaaa cagatcgata tagtggattt
     5581 tgctcgaaac aatggaatgg ctgtagtcaa taaaggacga gactatcgct tagaagacca
     5641 tgattcattt gtttttgatc gaagaaaaca acgtttttat tggaacagtc aaaatattag
     5701 tggcgacatt attgaattag ctaaactttt tttcatagac aaagagatcc aagactctaa
     5761 gcaacaattt aaagcagcca ctgattttat tctaaagaat gaagacaaaa cagaacgagt
     5821 ggaaaatctt cattttgaaa cagaaaagta caaagatcac cccgttgatt atcaaccact
     5881 cacagaaaaa ggaaggaatt atctgaagga agaacgaaaa ctaccagagt ggctgattga
     5941 ttatgcagaa aaagaagggt tgattgccga actaaagcca aaatatgaac ggcaaaattt
     6001 cttagttcga gacgatcgtt tagatcatgc ggtcgccttt ttatggaaag atccacagac
     6061 aaaagagaca gtaggggcga gttatcaagg aacgttcatt gattatgaac gttttggtga
     6121 gagaggcaca tataagcata tagataaaaa ttcgacagcg aatcatggct ttaacctaaa
     6181 gatcggtgac cctaagcagt taaaattttt tgagagtagt attgatttac ttagctatgc
     6241 tgcactgaat cgtgatcagt taaacgatac gtggttagtc tctatggaag ggttaaaaca
     6301 ccatgtgatc agtcattatt ttggtgaggc agtatctgaa ttgagaaaga aacaagcctt
     6361 tcctcaatcc atcgagattt gtgtggataa tgaccgtgca ggacatattt tctacgaaaa
     6421 agaacaatta atgggcgcag tcgatccatt tacgaatcag aaagtacgtt gtgaacgagg
     6481 aatcgctaat gattggcaag tgccaaaaga atacaaaatc atttatgaag aagtcgcaaa
     6541 agaagaaaag gtcacaccag aagctattat ggccatacac aagacagaaa acaatctgca
     6601 attaacgaac caactggttt ccgctcacaa agtaaatgcg ttttttggcc agcagctttc
     6661 tgttaacgat tcaatagaag ccattaattt gaaagatatt tgtagagaag tcgcaaaaga
     6721 attaaaagtt tgtgaacgcg tggatggtac gtacgatttt gatcgttttt atcaagaaaa
     6781 aggggatatt aatgcgcaga ttcttttctc ttataaagca gaacaatact acaaaggcta
     6841 taaaaatcat gaacatgaat ttatacctga agtaaaaaag gactggaacg atcaattgaa
     6901 gcatgagatc cagcaacaag aaatcagaaa gcaaaaacgt gccatgttgt ttcaacaagg
     6961 cagacaacaa gaaagggagt gaaatcgtca tgatgaaaac cgttatttta gcagaaaagc
     7021 cgtcacaagc gaaagcttat gccgatagct tctctaaagc cactcgaaaa gacgggtatt
     7081 ttgaaatcca agatcgattg tttcctggtg aaactgtgat cacgtatggt tttggtcatt
     7141 tagtcgaatt agattcgccc gatatgtacg atgaaaattg gaaacaatgg tcattagaac
     7201 atttgcctat tttccctaca cattatcact atcatgttcc taaagataag aaaaagcaat
     7261 tcaaggtggt gaaacagcaa cttcaatcgg ccgacaccat tattattgcg acagatagcg
     7321 atcgtgaagg ggaactgatc gcttggacga tcattcaaca agtaggcgcc gatcaaggaa
     7381 aaacctttaa aagactttgg atcaactcct tagaaaaaga agccatttat cagggattcc
     7441 agcagttacg agacgcaaaa gagacctatc caaaatttga agaagcacaa gcacgtcaga
     7501 tcgccgattg gttaattggg atgaacggta gcccactgta tagtttactc ttgcagcaaa
     7561 aaggcatacc aggaagcttt tccttaggca gagtccaaac cccaacgctt tacatgattt
     7621 atcaattgca ggaaaagatt agaaatttca agaaagaacc ttattttgaa ggaaaatccg
     7681 aagtaatcgc tcaaaatggc gcatttgatg cgaagcttga tccgaatgaa acccaagcga
     7741 cgcaagaagc atttgaagat tatttaaaag aaaagggggt tcaattggga aaacagccag
     7801 gcatgattcg ccaagttgaa accgagaaaa aaagcgcggc aagtccacgt cttttttctc
     7861 tatccagcct tcagtctaaa atgaatcagc tcatgaaagc tagtgccaaa gacactttgg
     7921 aagccatgca aggcttgtat gaagggaaat atctaagcta tccacgaaca gacacccctt
     7981 atattacgga aggggagtat gcgtatttgt tggatcattt agatgaatac aagcattttt
     8041 taaaggccga atcaatccca acccctattc atacgccgaa tagtcggtat gtgaataaca
     8101 agaaagtcca ggaacattac gcgatcattc ctacaaaaac ggttatgaca gcagcggctt
     8161 ttgaacaact aagtccactg cagcaggcca tttatgaaca agtgttaaaa acaacggtgg
     8221 ccatgtttgc ggaaaaatat acctatgaag aaacaacgat cttgacgcaa gtccagcagc
     8281 ttcagttaaa ggcaatcgga aaagtaccga tagatctagg ttggaagaag ttatttggca
     8341 aagagaacga cggaaaagag aaggaagaag agccgctact tcctaaagtg acaaaaggag
     8401 aaatggtgac ggtagatcta caagtccttg aaaaagaaac caagccacca cagccttata
     8461 ccgaaggtac gttgatcacg gcgatgaaaa cagctgggaa aaccgttgat agtgaagaag
     8521 cgcaatccat attgaaagaa gtggaaggaa tcggtacaga agcgacacgg gcgaatatta
     8581 ttgaaacctt gaaacaaaag gaatatatca aggtagagaa aaataagctc gtggtgacga
     8641 ataaagggat tttgctttgc caagccgtgg aaaaagagcc gctgcttacg agtgccgaaa
     8701 tgaccgccaa atgggaaagc tacttgttaa agatcggtga acgaaaagga acacaaacca
     8761 cttttctagc caatatccaa aaatttgtct ctcacttatt ggaagtcgta ccagggcaaa
     8821 tccaatccac tgattttgga tcgacgttac aagaagtgaa agcagccagt gaaaagcaag
     8881 aagccgcgcg tcatttagga atttgtccaa aatgccagga acaagaagtc ttactctatc
     8941 aaaaggtggc agcatgtacg tctgaagcct gtgatttcaa actatggaca acgattgcca
     9001 aaaagaaact tacagcgaca cagctgaaag agatcattca aaatagccga acaagccaac
     9061 cagtgaaagg gttaaaaggt cagaagggtt cgtttgaagc aacgattgtt ttaaaagaag
     9121 acttcactac aggctttgaa ttttctgaaa agaaaaaaac gaacttcaag aaaagaacac
     9181 gacgcactac aaaataatgg agggttaatc ttatgcgtaa tacaacacac aaacaacaag
     9241 aaatcgaaaa tcaaaaagcc aaaattcgtt cagaacagga aaaaatgcgt gccagcaaac
     9301 gaagactgca gcaattgaag aaccagcagc ggattcaaaa agaaatcgaa ttagatcgat
     9361 aaattccttt cttgttaata taaaactaac atgttatagt tgtgttagtt ttatattaac
     9421 tttaggggaa gagtaatgaa agacaaaatc caatgtattt ttgataaaca taatggcaac
     9481 ctatccttga aggatgctag aagtgaagga ttatcgccta tgacgttaga tcgattagcc
     9541 gaaaaggata aaatagaaaa ggtagcacca ggtttctata ccttaaagga tgcgttcgtg
     9601 gatgaattgt atctcgctca gagtatctat tccagaggga ttttttctca tgagactgcg
     9661 ctcgatcttt ttcaagtagg aacatatatc cctaaaaaga tcaatatgat gtttcctaaa
     9721 ggatacaatg tatcaaagga gaaattagat cagtatgcca ttcaaccgca ttacactaat
     9781 aaagaaaatt ttgacctggg agtcacagaa acagaaagct tttacggcaa taaaatttta
     9841 gtctatgatt tagagcgaac gttatgtgac atgtggaatc caagatacaa agcaagcgta
     9901 gaggtaaaac aagaagcatt aaaagaatat atgacaagta ccagtagaaa tacaaataag
     9961 ctaagaagat acatggatat tttaaaatca ccaaaagaaa tgactttgta tatgttgcca
    10021 ctttattgag aggagtaaag atgaatgaca ccgacacaat ttaaagagaa aacaagaaat
    10081 ttcgcagcag agaaacattt aaacccacaa attgtccaaa gatattatat gatggaaaaa
    10141 ttattagaat gtattgttga atcgccttat catgatgatt ttgtattaaa agggggattt
    10201 ctgattggat caaaatacgg gttagaaaac cgttcaaccg ttgatattga tacgacttat
    10261 cgaaattcga aactaacaaa agaaaagtta gaaactgttt tttctgaatt aacagctaat
    10321 cctacaaaag atggaattcg ttttgaatta aagggattaa cagagacgag ggaagccgat
    10381 tattatccag gttttcaagc aaagtttatt gcttatcttg aaaatgcacg tgtacctttt
    10441 aaattagata ttacttcagg tgattctatt attccagcag cattgatgta tgaacacaaa
    10501 ttaatgtttg aagataaaac gattcatatt ccagcttatc ctacggaaca aattctagct
    10561 gaaaaattac atgccacgtt tagttttgga aaagataatt cgcgcatgaa agatttttat
    10621 gacctgtata tgattccgaa attagaacgt atcgatgaga aaaatttgta taagagtgtt
    10681 caaggaacat tcaaggaaag aaataatcaa tcggtgttta agcatttgta tgatcaggaa
    10741 atgcctcaat tggtgaaaga tgagggcttg agagaaaagt ggaaaagata ccaacaagaa
    10801 aactattttg ccaaagacat ttctttcgaa tctacactaa cttccgttga ttccctgatg
    10861 caatcaattg tagagcaaga gaaggcagaa cgaaaacaaa attttaagag atcacaacaa
    10921 atgaatcaag aaatggagag ataaagatga tcaataatgt aactttagta gggcgattaa
    10981 ctaaagatcc agatttacgt tataccgcaa gtggcacagc agtggcgact ttttccctag
    11041 ccgttaatcg taatttcacg aatcaaaacg gcaatcgaga agctgatttt atcaattgcg
    11101 tgatttggcg caaaccagca gaaacgatgg cgacattagc taaaaaaggc agcttgatcg
    11161 gcgttgtcgg acgaattcaa acacggactt atgacaacca gcaaggacaa cgtgtgtatg
    11221 tgacagaagt tgtggccgat aacttccaac tattagaaag taaagcagcg acagaaagcc
    11281 gtatgcacgc tgatcaatcg tccagcagtc caagccctac cacctttgaa caacgggata
    11341 cagcgacgcc taacaacaat ggattgaacg cttcacagaa tccatttggt ggacaatcaa
    11401 tcgatattag cgatgatgat ctaccgttct aagtagcatt ttctattggt tgcatttgct
    11461 acggtttatg ttataattta gacaacaaaa agaattttca atctataacc aaaaaccact
    11521 agctggcggg ctagtggctt tcttttacgt ttctattata agaattaacg ggtaggctta
    11581 ctgtctttct tggcaaattt acaatcaagc catttgcaga tatagtgcgc ggctacttct
    11641 gccatgattg caaaaacaaa atcaaaaaga attttcaaac ctataacctc ctccccttgc
    11701 cagattaggc cggggtagca gacagcaaga ctattatagc ataattgaaa tatccaaatg
    11761 attggatttt tgctataatt ttagcgaggt gattgtgatg aacaaatatg ttggtttgct
    11821 tgataaaatc cgagtaataa aaacgcaacc gttattggtt cgctttactt tacaaacgat
    11881 tcatgaatcg attaattgtg tagttgcaga tattgaaatt atcgataaat tgctaattat
    11941 ggatgatgga aaatacaata ttgccgtcac aggtcacttc aataaacgaa atcagctagt
    12001 tattgctagc atgtatgtta gaaatcccga tcattttacg cgatcgatgg gcatataaat
    12061 aaaagaagag ttgtagtcct aaatgaggat tgcagctctt tttgttttac atagaaacag
    12121 gaaaagacct ttttaggaag acacaagtgc cttctcaatt gaaaaacctt tctctcactc
    12181 caaccgccac cgccgttcga gaagaaagga agtgaacgct gcacgttcac taagatatgg
    12241 tgtcttgcaa agcaagacaa aatagacaaa acttcctctc accaaaaggt tcgttcctgt
    12301 tcgttatctc tttattgatt tgccagaccg gcaacaagtt gccttttatc accaaaaagc
    12361 gacaaggaca agggtaaatg gcacgctgcg cgcccttgtc cttgtcgctt ttcgttgatt
    12421 tattctggcc aacaaatcaa taaagaaaga actaacgaaa ggaagtaaaa cctttcctaa
    12481 gaggaggcag aatcaaacat gcgaaagaaa tttgattatt ggggtgttcc cttcagcgaa
    12541 ttattcccaa actatcatgc accacatact gttgaatgcg attgtggaga aagagcgaaa
    12601 tgtatcaaaa gctatcgttt gtatcaatgt ccaacatgcg ggaaaaaata taccttgtct
    12661 tatggcgatt acattttgat tgatgagaaa ggaaagaaac gctgatgaaa acacaacaca
    12721 aattattgac tgtaaaagaa gtcgttcaaa ttttggtgga caaagaagtc atttctgaaa
    12781 aagtagcaga agctttactt attccgcggt actggacgat agaaacccct ttagtagagg
    12841 atgcaccgac tatgctattt gaagacctag tgaagattgc aggatatgaa ctgcattatc
    12901 aagaaaacat ggaaacttat gaaatcgaat attggttaac caaattagag aactaaaaag
    12961 gagaaaaaat atggctcaaa attactatga cgaatttgtc aaattacctt tggataaaat
    13021 ggcacaaaaa atggaggata tgacgttcct ataccatgaa acacgtgtac ccaaaaaaca
    13081 ttacaaggaa aaattgtcag tcgcagtgga agaaatgatt gaatcaggtg tcgaaatgaa
    13141 tctcattgcg acgtactatc gaacactgga agaactgaaa aaacaaaatg gaaaatggtt
    13201 tttccaagcc ttactatgct tggaagtagg tgtcaaacca agcactatca aaccttccga
    13261 atatcaagcc ttagaactga cttatgcaaa attcgcacag attaagaaag ctaaagcaat
    13321 ggctagcgac tggctagata cttttgaaag tatcgaaaaa aatggtgcat tttatacgat
    13381 tgaatcggag gagaaagata atgaataagg tggtttatca gctaacaaca gaagatgttt
    13441 cgcaattttt aacaagcaaa gaacacttga atgaagtaat cagacggatt caaaaagact
    13501 ttagttgtat ggaagaaatg aagtacttta ttcaaagagt ggaagaacag ttgtatatgg
    13561 aaagcgaaac aagtcaaacg aaggtcacag tcagccataa tcatttagac gtgtataaac
    13621 gtcgtcctga aacggtaaaa aaaggaacag agcttctacg actagaaaca gaaaaataca
    13681 tcatgaatgt gtacctagaa aggagtgagc tggtcgaaaa ctttgtcagc tacacgattt
    13741 gtaggaaaca ctattactca gaatcagaat attattcaat tttcgagagc atggaattaa
    13801 tggaagaaga aatggaagag ctaaacagca aaaatgcgac ggtagacgat tattaccttg
    13861 tatttactag gtttttgacg tgtgggacgg aatctatcgt gttaatggga atggattatg
    13921 aactcgctgt acccgaagca tttttgaggg aatatgtaga agaagtagat aacccaggcg
    13981 acgactggta cacgacttta actgattttt tagacaccta cacttacgac gatacagaag
    14041 aaatccaaaa tgaacttgac cgtaaacgaa tgccatatca acgatttgaa aggaaaaaag
    14101 gtaaataacc atttataccg aaaagtgagg atgtgtgaaa taagaacaaa aaaccttgaa
    14161 attaagggaa tggttcttat ttcgcaccaa tcgctccaac cgcccccgcc gcttaggtgg
    14221 tgcgaaataa gcgggcaaag cccgataacc taagagaaag aaggagatcc atatgaataa
    14281 aatcattcct ctattgttcg tactgtttgt tggcgagata cttatgcgtc atgctgcagc
    14341 tgtacattta aacttcgtag tattcttagg aatactggta ttttgtttga tcttttggcg
    14401 atttgtatta gcccttgtga gtctactatt caagatgatt gcagccatat ttttcatagg
    14461 gttatttcga tgaaaggaga gagtactaat gccaattgca ataggaaata aacggttacc
    14521 cgtgacatta gatgaaaaga gacaaaaaga attgcagcaa ctaaagcaga agtacggaaa
    14581 aagtgaatct aagattatgt gtattgcgtt agatctattg atcgcgcaag aaaaag
//
LOCUS       DAHHZJ010000058        14459 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.059, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000058 DAHHZJ010000000
VERSION     DAHHZJ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.059"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(269..631)
                     /locus_tag="NBL02_001430"
     CDS             complement(269..631)
                     /locus_tag="NBL02_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572121.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(705..1085)
                     /locus_tag="NBL02_001431"
     CDS             complement(705..1085)
                     /locus_tag="NBL02_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HCD2572122.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1149..2342
                     /locus_tag="NBL02_001432"
     CDS             1149..2342
                     /locus_tag="NBL02_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCD2572123.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2418..3002)
                     /locus_tag="NBL02_001433"
     CDS             complement(2418..3002)
                     /locus_tag="NBL02_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCD2572124.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3203..4201
                     /locus_tag="NBL02_001434"
     CDS             3203..4201
                     /locus_tag="NBL02_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCD2572125.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4313..4822
                     /locus_tag="NBL02_001435"
     CDS             4313..4822
                     /locus_tag="NBL02_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCD2572126.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4852..5511)
                     /locus_tag="NBL02_001436"
     CDS             complement(4852..5511)
                     /locus_tag="NBL02_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HCD2572127.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5513..6277)
                     /locus_tag="NBL02_001437"
     CDS             complement(5513..6277)
                     /locus_tag="NBL02_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HCD2572128.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6285..6962)
                     /locus_tag="NBL02_001438"
     CDS             complement(6285..6962)
                     /locus_tag="NBL02_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HCD2572129.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(6999..8384)
                     /locus_tag="NBL02_001439"
     CDS             complement(6999..8384)
                     /locus_tag="NBL02_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCD2572130.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8594..8678)
                     /gene="ffs"
                     /locus_tag="NBL02_001440"
     ncRNA           complement(8594..8678)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="NBL02_001440"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8701..9216)
                     /gene="tadA"
                     /locus_tag="NBL02_001441"
     CDS             complement(8701..9216)
                     /gene="tadA"
                     /locus_tag="NBL02_001441"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCD2572131.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9323..10180
                     /locus_tag="NBL02_001442"
     CDS             9323..10180
                     /locus_tag="NBL02_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD2572132.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDGTEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10261..10596)
                     /locus_tag="NBL02_001443"
     CDS             complement(10261..10596)
                     /locus_tag="NBL02_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572133.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10663..12033)
                     /locus_tag="NBL02_001444"
     CDS             complement(10663..12033)
                     /locus_tag="NBL02_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HCD2572134.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12111..12647)
                     /locus_tag="NBL02_001445"
     CDS             complement(12111..12647)
                     /locus_tag="NBL02_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCD2572135.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12956..14437
                     /locus_tag="NBL02_001446"
     CDS             12956..14437
                     /locus_tag="NBL02_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD2572136.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4455 a         2881 c         2353 g         4770 t
ORIGIN      
        1 aatgtgatat aacatccact tcagaatcaa cgctgttaaa tttatcatgt atttcttcct
       61 tggtcaacta taaattaagc attactttgt gaaatgattt ttcgatcaat tattattatt
      121 cgttatacaa ccgttttcaa catattttga aaataaaatg cactaaaaaa ctatctttag
      181 cagaacattt cattgtattt tttggaccaa acaataaaaa aagaagtttg aaaaaaattc
      241 aaacttcttt gttcgcttaa tctgtatttt agaagaaagg cactccctct acttcgtaag
      301 ttccaagaac agagcgcatt tgacctggtt cttcccaaat atatccttcg cctaattctt
      361 ctctttgatt cttttgttca gtttcaaaga caaacaacgg aacattttcc tctttaatat
      421 gtgtatttgt caattccatc aaactaatct tgctaacatc gatatgaact atttctttta
      481 agaaattaag tatattcgct aaaccagtct ttccttcaga aagttccgct aacgcaaatt
      541 ccaacttgtc atcaattgaa tggaccaaaa attttttagt accatcctcc aaatgcaaca
      601 taattgtacc agcaactctt ttgcttgcca aaatttccac ctcgatcaac ttgttctatg
      661 gcttacataa aaattctagc acattttcag aaaaaataca ctttttattt cttattttct
      721 ttccgttcta gttcagataa tgcttcttct atccaaatag gtaactgctc cgcaatagtg
      781 gtaaatccga tcttacgatt tttattcgtg aaataccttt ttctgcgata tggaacagct
      841 ggatttgttt tttctaacgt tcttaaagat aaactgattt ttttggaata ttcatcaatg
      901 tcgatgatct gaactgttac tggctgtccg acttttaaca atgaatggat atttttcgta
      961 taaccggatt gtacttctga aacatgaata agtccttgcg tctcgttatc aagagaaacg
     1021 aatgcgccat aaggctgtat ccctgtcact tttccttcaa ttatttgtcc aattttataa
     1081 gtcatctact tccctctctt cgcatttatc attatactgt aaatagctca aaaaaagaag
     1141 cgacgaaaat gactggaggt cttaagatga tcgaatttga taaacagatt tcaagaaaag
     1201 atacagaaag tgtaaaatgg gacgcaattg ctgagacata tcaaatggat gacttacttc
     1261 ctttgtgggt agcagatatg gacttcttgt ctcccggtgg ggtagctaaa gcgtttagtg
     1321 aatacatcaa acatggaatc tttggttatg ccactctgtc tgataaacta tatcaggcta
     1381 tcattcattg ggaacgccaa caccatgctg tggagttgac gaaagaaaat atcgttttta
     1441 caagtggtgt gttaaccagt ttagctgcag cagtacaaac ttttacaaaa ccaggcgatt
     1501 ctattttgat tcatgaccca gtttatcctc cattttcctc tattatagaa acaaatcaac
     1561 gtcacatcgt tcgaagctct ttgctagaaa aaaataatca tttcatcatg gacttgtctg
     1621 acatggagaa aaaaatcgaa gaaaacaatg taaaagcaat gattttatgc aatcctcaca
     1681 atcctggagg gcgtgtttgg agcaaagaag aattgaaaca gttaagcgaa ttgtgtttaa
     1741 aacatcaagt gattctgttt agtgacgaga tccatcaaga tttaacttta ttcgatcata
     1801 catttacttc catgctaacg attgatccta atttagatga tctactagtt gcttttactt
     1861 ccgcaaccaa gactttcaat ctagctgcaa tcaaaaattc aatggtattt atcaaaaatc
     1921 cagagatgaa agctgatttt gaaaaacatt tagtgatgaa ccaacagcac gaaatcaaca
     1981 cttttggact gattggaacc caagcggctt atgagacggg tggagaatgg ttaagccaac
     2041 tgattcctta tctagaaaaa aatgttcaaa cagttaatca gttttttgaa cagtatcttc
     2101 cgaaagtgag aataatgaac cctgaaggaa cttacttaat gtggttagat tttaccgcct
     2161 atatttcaga cgaccaagaa ttggagaaca ctcttgttcg gaaaggaaaa gtcgtattaa
     2221 atcctggcat cacgtttggt ccttccggtc atgggcatat gcgcttgaat attgcttgtc
     2281 cagaagaaac tttactggaa ggacttaatc gaatcaagta tgctttagag acaatcgatt
     2341 aaatatttat tcaaaataaa aggacgagac cttactaggt ttcgtccttt ttcataatta
     2401 ctccgtacca taccaaacta ttctgaaatt tcaattgttt caatcactac atcatgtaca
     2461 ggtcggtctt gtggaccacg ttgtacattt gcgatttcat ctactgtgtc catcccttct
     2521 agtacatgtc caaagactgt atgtcggaaa tctagccaag gagttccgcc acctttatac
     2581 gcttcaatga tttctgatgg gaatccagca ccttctagtt gtccaagcat attagctggc
     2641 acatttgtgt tatttacaat aaagaattgg cttccgtttg tgtttggacc tgaatttgcc
     2701 atagataaag ctccgcgaag gttgaacagt tcacgactga attcatcttc aaaactttcg
     2761 ccataaatac tttcgccacc cattcctgtt ccagttggat cgccgccttg gatcatgaaa
     2821 tcaggaataa cgcggtgaaa aatcacgcca tcataataac cttttttgga aagttcaatg
     2881 aaattttgaa ccgtcttcgg tgctagttca gggaataatt gaacagtaat gtcaccacgg
     2941 tttgttttga taacagcttt tggtcctttt tcacttttta aatctagttg tgggaatgcc
     3001 atattgtttc ctcctaatat ttgcatactt cataataata ataacataat acctaacctc
     3061 tcgtaaaaat actagatttg ttttctttta tttttcatca ctagatacat tctataactt
     3121 accgtttcga tttcgaattc aaacattttt gagaagcatt ctcaattaaa ttctgctata
     3181 gtgtgaaaag ggaggaattt ttatggaaat ctatgacatt acaatcatcg gtgcaggtcc
     3241 tgtagggatg tttgccgctt tttacgcagg aatgcgccaa gctaaaacaa aaatcattga
     3301 cagtctacct caacttggtg gtcaattagc aactttgtat cccgaaaaat atatttacga
     3361 tattcctggg tatccagcaa taaaagcaag tgaattaatc gatcaattag aaaaacaatt
     3421 aactactttt aaccatacat tccatttaaa agaagaagtt ctctcgctta ctcgtgaaga
     3481 cgaagttatc gagattacta caaataaagg catccattat tccaaagcag tcattttagc
     3541 tttaggaaat ggttcattcc agccaagaaa attgaattta gataatgctg aatcatttga
     3601 aaaccacgga ttggattatt tcgtgaatga tttgatgaag tatgctggaa aaaaagtcgc
     3661 tattgctgga ggtggtgatt ctgctatcga ttggacgttg atgttagagc ctattgcaag
     3721 cgaagtttac ttgattcatc gtcgcccgga atttcgtgcg cacgaacata gtgtatcacg
     3781 cttgaaatct tcatctgtca acttgcttac tccctatctc atcgatggat tatcaggcaa
     3841 taatggggaa ctgacagata tccggttaaa aaaagtgaaa agtgacgaga cgattgattt
     3901 gatgattgat tctctgatcg tcaattatgg attctcttct aatttagaac atctttcttc
     3961 ttggggactt gatagtacca gaaatgccat tactgtaaaa tcagatatgt ctacttctat
     4021 tccaggcgtt tatgcagctg gtgacatctg tacctatgaa ggaaaagtga aactgattgc
     4081 tactgggctc ggtgaagcac ctactgcagt gaacaacgct ttacactata ttaatccaaa
     4141 ggaacgaact cagcctggtc acagcacaag cttatacgat aaaaatcttc gtcctagttg
     4201 attttttata tctctttgca tttcaagtta aacccgaaag cagtttcttg catttttttc
     4261 atgttccgtt aacatgaaat agagaaagaa aaaataaagg gggagcaata atatggtcgt
     4321 caaaactgag actttagaag aaaaggcaag agaactattg aaggaacgtg gcgtaactgt
     4381 agaagatatc gcacaacttg tcatgttttt acaaaaagac tatatttctg atttgacttt
     4441 ggcggattgt actgaaagtg tcaacagcgt gctaacaaag cgtgaagtac ataacgccat
     4501 tatcactgga atccaattag atatattagc agaaaagaaa aaattgatga gtccgttgca
     4561 acatattatc gaagatgacg aaggccttta cggtatcgat gagatcctag ccttgtcaat
     4621 cgtgaatgtt tacggtacga tcggctttac gaattatggt tatatcgaca aggtgaaacc
     4681 tggtatttta aaagaactaa acagccacga tggcgttcat gtccatacct tcttagatga
     4741 tatcgtcgga gcgatcgccg ctgctgctgc cagtcgatta gcacacaaag aaccagaaaa
     4801 aagaagtcgc cttactcaat aattcaacaa aaaaagaaag aaaacgtcta tttagacgag
     4861 ttctttcttt tttgtttgtt tcaagctatt ttttcctaag aaaagaaaaa cagcaaaaaa
     4921 taattctaat aatacaaaaa ataagataaa gctatgcatg aaaaattctt ctggaagtac
     4981 attgatgatt ggatcagtaa cgggatcgaa taaccagtca tcgttgttga aaaacgtttc
     5041 atgaaaaagg ataaaaaagc gatcaaatcc tatcatcatg aaaaaaccga aaacaactgg
     5101 cagtaacatg ccgatttgaa acggacgaat caaccgccaa agccgatcat tcttttgcaa
     5161 atatttgata aacaaaatac tcgggatcaa agtgatgaaa aacacagcat aatccactaa
     5221 aaacaaaatt ttcacttcat aaaagtgatg ggctccgcta gcagaaactg ggaaatctgg
     5281 caagctgagg atggttttga agggattatt gagataattc attaggtgat caaaattttt
     5341 cagtaacgtc tcttgatcta aagaagtata atccaaaatt tgcaaatgtc cgatatcaaa
     5401 tacgtatagt ggtcgaaagt tgatcgtcac accaactgcc agactgatca gtgtcagaaa
     5461 taggctgaac aatccagcat attccatcca ctgccatctc gaatttttca tattagaagc
     5521 tccattcatc cagtgattgt ttcacatagg tagctgggat aggcaaccca ggtacatctt
     5581 cttcttttgt aaaacctgat aaaacgagca aagtatctat attattgcgt atcccagctt
     5641 ggatatctgt ttcatagtta tctccaacca tgattacttc ttctttttgc agccctaata
     5701 cttcaacggc tttatccata atgatggctt ctggttttcc gatgtagatc ggcggcgttt
     5761 gggttgctgt ctctacaaaa gagatgactg accctgctcc aggaagaagg ccgcgttcag
     5821 taggtatatt cttatctggg ttcgttccaa tgaaagtagc acctttttga atagcaagtg
     5881 ttgctttaac tactttttca taggtcagat aattatctaa gcctaccact acatagtcag
     5941 gagcctcttc ttcccatgta aatccggcag ctaaaatcaa gtcaatcaat cctgcttctc
     6001 cgataacata gacttttttc ccttttccat ctgctttcat aaaatcgatt gttgccaaag
     6061 tagctgtgta gactgtctct ggagctacat gaatgtcaaa ctcatctgct agacgattag
     6121 ctactgtttc tggtgactta gtcgtgttat tggtaacaaa caagaaaggt aattttcttt
     6181 tttgcaattc ttccacaaat cgtttaccgg ccggtatggg ttgtgtccca cgataaatcg
     6241 ttccgtctaa atcaattaaa tatcctttat agtccattca tccactactc ttcccttttt
     6301 ctgatcgtaa atgctgtttt tgttttttct acttgtttag gttgttcttg tttttttact
     6361 ttttcagagg tttttgctga ctctgttttt cgatttttaa taaccggttt tttgcgatta
     6421 gatgcgactg gtcctttttt ctcatcgata tacgcacgat tatggttttt tttctttttt
     6481 cggcgctgag ttgtcttttc ttttttaccg ccgattctct cgatgacaaa atacgcacaa
     6541 ccaaaattgc aatattcgta aagatagtct tccaaggaat cgattcgctg atctggatgc
     6601 gcccgtcgat tatccgcttg gaaaaatcct tttaggcgta gttgttcata accccaatct
     6661 cctacgatat aatcatatcg ggctagtaca tcactgtaac gttctccaag cttttctgca
     6721 ttaaatcctt cacgataatc ggacacaagc cgataatgtc tttcacctat caaaaaatta
     6781 ctgtcatcta acagtgtcac tagctcgcct ttgacttttt ctgctttagg ctcctcaacg
     6841 atttcttcca aagcagctgt cagttcttcg gtgactgttg tttcctgttc agctgatgat
     6901 tttttcttag caatttctgt tttatgctca acgttctgct gttcattttg ttttgcagac
     6961 atgcgttcac cacctatctt ttttattata ccttgttttt atttgattgg gtagtgagct
     7021 ttcaagtaat ctcctaccac acttcggata aattcaggga acaaaaattc attttttcca
     7081 gcaatctcaa tcgttggaaa aaatggaaca aacatcaaat gatctacggt agtaaaagta
     7141 taatttttat tcagatcgat tggcttgttc ttccaaaaga cttgatggtt aatagaatca
     7201 tatgtgattc cattatagac catctgccca aaaatcttac cgcgaaatcc cattcccttc
     7261 ataggatagt tacgtaaaaa gttacgattt ttttctattt ctaaaactaa gcggatcaga
     7321 tcatttccac ccaatgtgac atttaacaag tgcatcggat gcggcaacgc agtgtgaagc
     7381 tgatcctgat tgacccgccc ttttggaatt tcagttaaaa acagaccact gtttaatatt
     7441 gctgcttccg tctttcctcg ttctttcacc gcctcaaagg ctacttggat aaacgaatgc
     7501 tcattgaaga tatctagact cagagaaaat ggcaaatctg ctacttcttt ttcttttaat
     7561 aattcatgcc cacgttctag ataactattt atttcttgtt catcttctgt aaaagctgtc
     7621 attgtttcgg tagggatggc tttggctgaa tgcttaagga tttttttctc ttcatcgatg
     7681 gttaaatgga cttcaccgac atactgcccg tactttccag cggctgctat ttggacatca
     7741 ttgattacgt gaccttctct gaataaatga tgggtgtgcg atcctaaaat aacatcaatt
     7801 gctggtattt cttttgcaat ttggaaatca tcctgaatcc cgagatgact catcaaaact
     7861 aaaacatcca cttgtgaacg taaatcttcg actaattctg gcaaaatatc ataaggattc
     7921 cgtatgtccc agccattcgg attataagta agcggaaatg gagcagtaaa tgcaattagt
     7981 ccgattttcg tctggtgttt cgttgtaatg attttatatg gtttcgtcca tgccggtggc
     8041 tggagcgtat ttttatcgaa taagttatcc aggatgatat caaactcagc atgatcgtat
     8101 aaatgattca actcttcttt ggaatttcct acgccttcat tgtttcctat cgtcgctgca
     8161 tcataaccaa cttcattcat cagcaagata ttttcctgtc cattcgttgc ttctgacaat
     8221 ggatgccaac gatccacaaa atcacctaga tctactgcaa tcgttgtcgt tttttctttc
     8281 ttttcgtttt ctcgtttacg ttgatccaaa aaccttctga tccttggcca attttctaaa
     8341 tgagaatgca gatcattcgt atgtaaaatc actatttttt ccatgtaccc tctcgcctct
     8401 ctttatcttt atcataccat aggcagcttt ttcttccatt gaaacaagct ctatttcaga
     8461 gtttttcctg ccttgaagca aattgtataa gtacaatatt cgataaatca gtctggctaa
     8521 aaaatcagga acattcattt ttacagtact ataaaaataa aaaaaacaga cccgaaagtc
     8581 tgttgtcgtt tcattataat cagacacatg gcggaacctg cttagtgctg cttccttcca
     8641 gacctgacac gcttcacaag cccaccattg tcctagcctt tcggcaaaga atagtatacc
     8701 ttatttttca ttcgttgacc agtctttttt taatttcttt tttcgctggc ggagctttcg
     8761 gaaaaagtca gtcaaaatca tcccacattc gtcttctaaa atgccacctt ctacatatgc
     8821 ttggtggtta aaccgctcgt cctccaaaag attcatcaag gtaccggctg ttccgccttt
     8881 tggatcatac gcgccgaaat aaacttctgc tacacgagac aaaatcatcg cgccgctgca
     8941 cataggacaa ggctctaagg taacaaaaag tgcagcatct tccaagcgcc agcttcccac
     9001 tttttcacag gcttccttga ttgcaagcat ttctgcatgt gtagtagcat cttgcgtggt
     9061 ttctctaagg ttatatcctc ttccaatcac ttttccatcc aatacaacca ctgctccaat
     9121 cggaacttca tgaagcattt ctgctttctt tgcttctgcc aatgcaaggc gcatccattt
     9181 ttctttttct actgcatcaa gatttgtatt taccaattac ccttccctgc ctttgttttt
     9241 tcttgtttta ctttagcata tgataaaaag gctgtaacta actttttttt ctatggtaaa
     9301 ataaatacaa gaggtgaaca agatgaaaat ggataaatta cttgctcttt acccacaagc
     9361 acagaaaaaa aactctccag catcagaagc ggatatcctt tcttttgcaa ttgatgatgc
     9421 ttttatctgg atcaaacaag attcattatc tcaacaagaa accagccttt tgaaagcgtt
     9481 attccctgta atcaacgatc aaaaaaaaca cccatggtat cgctatcttt ttaaagatgc
     9541 ttcttgtgtt caagaaaaaa gctttcgttt gatccagctt cacgtcgaat caaaaggaga
     9601 attcttaaaa aaagaatggc aagatactat tttggaatta tttcgctatt ctgctgattt
     9661 ctttttttat acagacacag atgcgctctt agtcgaacaa ataaaccgtg attatcttga
     9721 tggtactgaa atcaacggta tttttctatc tttagatgct gattttgata cgacaacgtc
     9781 tgtattcgtt ggttctttcc attcacctgg caccgacctc gtccctttgt ttgctgaaga
     9841 aagaaaaatt tttttaacag agcaaaacaa tctttatact cacagccgta ctttttctat
     9901 gcaagatgtg gctttacatt actatacaaa agatactgta aaagatagta agttgatcaa
     9961 aagttacata gaactgatca aacaaaatga aatggaagtg atcattcaag aattatggaa
    10021 tgaattaggc aatgtcagct ctgctgcaaa aacactgttt cttcatcgta atactctaaa
    10081 atatcgaatc gaaaaatttc aagaacagtc tggtttcaat ctaaaaaaag ccaacgattt
    10141 actgttttgc tatcttttat tgcttcatga acataattaa agatagatta aaggggttgt
    10201 gacaataatt ttgtcacaac cccttatttc gaatgaatag tgttcaaaag ctagtttctc
    10261 ttacatcatt ttttcaacaa aatgcattag ccggtctttt acatcttcgg ttgaatcttt
    10321 gattgaatca ccataaacag agattttctt ttttcctgat ttgatttttg ctaacattcc
    10381 catcaaagaa tccaagtcac tatctgaaag accatctacg atttctagta atttttcatt
    10441 tccatcaaat ttgtcataga cgaatttttt tactttcgtc cgagtagtca tatgatggat
    10501 cttttccatt acttttcccg atacaacgac tgctgtcgct acactggcag cggctgctac
    10561 gcttaatccg attgttactt ttgttgatgt tttcataagt atcagctcct ttttatctat
    10621 ctccatcata gcatagacaa aaaaataaac gaactaatcc actcagtgaa atctcaatcc
    10681 tttagggaaa aagttcttta ctgttccttt tacttgtttc ccaaatccga caggtatctt
    10741 atctaatact agcaaaaccc atccttgatt cccatcacgt tgaaatgttt ctcctgctgt
    10801 gtaagattgc cattcctttt gagtgatcgg caagcaaggt atattctcta tcttttttgt
    10861 agcaagcgct aatgcatagc taggttcaaa tcgattcttt ttgaaatctc ctaaatgcaa
    10921 acctgtacgt acgactttta aaccatcgag agaaggggct aactcaggaa cttcccaaag
    10981 atggtcgttg aacactaaaa gtcttccagt agcttcataa tgaaaatcat ttgaaaactc
    11041 cgtccagagt ttttcctgct cttttgtcat ttgcacttta cttttttttc gtgtcttctt
    11101 ttctttatgc atctgatttt gtccatgaaa agtcagcttt gcaacaaaat gtccttcacc
    11161 ctgatcttta tgaggccata tacgtattgt tttttccaaa ccagctacgc ttccccattc
    11221 agaacgtcct gaactaacgg attgagtcaa aggaatctcc tcaatcgtta ctggataatt
    11281 ttctacaagc cagctaatga tctcttcatt ttcttctggt gcaaacgtac aagtcgaata
    11341 aatcagttgt cctttatttt ttagcatttt tattgctgaa gacaaaatct cttgctgacg
    11401 tttttgacag tatagcggag attcttctgt ccactctttt attgcatttg gatcttttcg
    11461 gaacattcct tctcctgaac aaggagcatc aaccacgatc cgatcaaaaa agcccgaaaa
    11521 atgagggaca agttctgcgg gtgcatgatt tgtcactatt gcgttcgaaa ctccccatcg
    11581 ttctatattt tctgataaaa tcttcgcacg tttcggaaag atctcattcg tcactaataa
    11641 ccctttcccc ttcatttgag cagctaattg ggttgattta ccaccgggag ccgcacacaa
    11701 atccaaaact ttttcaccgg gttttgctgc agcagctgtt ccaacgatca ttgcacttgg
    11761 ttcttggcta tattcatacc cagcttgatg gaggaaagac tttccattga cagtgccaag
    11821 aaatccctca ttgctgtagg gagcgggttg taattcttcg ctatggtaag tttggaccat
    11881 gtccaatcct gccggtttta gtggattcca ccgaaatcct tttttcacgc ttccttgttc
    11941 aagcgcagaa aaaaaatcgg atgcttcttc gcctaaaaga agacgatatt ttttgataaa
    12001 ttgctgtggt aacgttgtcg cttcctcctt cattccaatg tttcgttaga cattttacaa
    12061 tcttttttac gaaaaataaa ggtggctgat ctactttttc atgttgctgc ctaaggtgac
    12121 tgttccttta agatattttc caagtattca tccgtcacct cgtttttatc ccaaacagcg
    12181 ttcatcattt caagaaaatc ttgagaagaa tcatctgagc gcaaacttcc caatgcatta
    12241 agccatacca attcaagcga cttttcttct ttgatcttcg tgtgaccttt gacatatgag
    12301 aaaaatggcc gcttaaatag cgaatccatg tctaaatcag attttgcaat gatttcacga
    12361 atactttcat ggatagaaaa aaaatctttc aataagtaga caatgcttaa aacatcatcg
    12421 gaactgcatt cagctaagta gttttcaaaa taaacagtga tttcttctaa tatcttctct
    12481 tttaacccgc tgattgattt gaaatgtgta taaatcgggt atgtagaaca accgcagaac
    12541 tctgctaact ctcttgcagt aattgacttc attccatatt cttccacgta taccatactt
    12601 ttgaacagca aatattcttt actataattt gttactctcc ccatcattct tacctccgtc
    12661 tcttttcatc tacccataaa taaataacat aaataattct aaaaatatta ttgataatta
    12721 tcaaaacata tttagttata tccattaaaa atcaattttt ccgccttaaa aacaagaata
    12781 tcttgacaaa ataccctagg aaataaacaa tagaataaac aaggtaacaa aaaaattaaa
    12841 taacgttata taaaaaagaa ggcgacactc tgtgagaata acacagactg aaatgacttt
    12901 ccactcacag tttttgtcta actatataca aaaacagaat aaaaaggagg tatttatgct
    12961 aattgaagca agaaaaaaag agttacttgt tctacaaatg gtcattcaaa aaaataattg
    13021 ttctttacaa gaattatcca atgacttaca tattccaaaa cggacaatca aagaactgat
    13081 ccgaaaaata aatgtatcac tcaagcaatt tttattgatt gatgaattta tttattctaa
    13141 tcataaagga gagatccaaa ccaaagaaac cgatcaaact aaaaagttag ctgttttttc
    13201 taagttaaaa ttatgctacc taaaagaatc caatcgtttt aactatttga tgttactaat
    13261 caattatcca caaactgctg taccaaaaaa atatttactt gaacaacttt atatatcgcc
    13321 ctcctatctc gaaaaactga caaaacagct caatcattcc ttaaaaaaat tccatttacg
    13381 gatcctatct gtaaaaaatt gctgtactct tgaaggaaat gagttatcta tccgtctcta
    13441 tctttacttt tttctttcag atacatttgg aggtaacgat tggccatttt cctcacttga
    13501 attaaatgca ctaaaaatga acagtacaga tgagcaaata gaaaaaattt caaaacaaga
    13561 aaaaaatatc tacttgctgt taactttatt catattacga aacaaacaac aggcatattt
    13621 tccaaaaact tcttctgaaa tcactgaatt gatggactta ttgcagcata cacatgatta
    13681 ttcagaatac tttacttctt tttcagtgag tcgcccttgt gaagagacag aaacagagag
    13741 agcctacttt aattattttt tctattgttt ttttccaaat caaatctctt ttcgacaaaa
    13801 acaggagatt ggttttttat tttccacaag caaaaacctt atttgtcgac aagtcggtca
    13861 attgatgact tacttaactc aacagtttcc tctcaaccat ttagaaaata aaataccaat
    13921 ttatttttat tttcttgtgt tggcagttgt ttttttcgaa ctgttgggtg aatccagcag
    13981 tagtttttct acactttgct ttccagaagc cacgtccaat cattctagtt cttttataca
    14041 ggaaaagaaa ttaagagaaa aaatcgatac aattttttca aaaaatgggg aaagaaacca
    14101 aatcagtcaa taccttagtc aattgatcca acacttacta caaacagaaa cgaaagagca
    14161 gttactcatc catttgcaga taaccaaaca gtttacagga aattatcatc ttcaaagcca
    14221 tttgcagcag ttatttcgca aagatgctgt taaaattacc gcatattcag aacatgctga
    14281 tttgatcatt acagatcagc atgagaaaat ttcagatgaa acggagactt tctatctgga
    14341 tacaacgcac aatacagtgt tatttggcaa gttaataaaa gcggttcatg aactgtatat
    14401 gaaaaaaggt tatgagaaca aaacaaagag gatgtgacaa aattcttgtc acatcctct
//
LOCUS       DAHHZJ010000059        14301 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.060, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000059 DAHHZJ010000000
VERSION     DAHHZJ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.060"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(<1..534)
                     /locus_tag="NBL02_001447"
     CDS             complement(<1..534)
                     /locus_tag="NBL02_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCD2572137.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQP"
     gene            complement(534..1490)
                     /locus_tag="NBL02_001448"
     CDS             complement(534..1490)
                     /locus_tag="NBL02_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCD2572138.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1490..2146)
                     /locus_tag="NBL02_001449"
     CDS             complement(1490..2146)
                     /locus_tag="NBL02_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCD2572139.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2516..4534
                     /locus_tag="NBL02_001450"
     CDS             2516..4534
                     /locus_tag="NBL02_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCD2572140.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    4604..4810
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4961..7375
                     /locus_tag="NBL02_001451"
     CDS             4961..7375
                     /locus_tag="NBL02_001451"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCD2572141.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            7723..9276
                     /locus_tag="NBL02_001452"
     CDS             7723..9276
                     /locus_tag="NBL02_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HCD2572142.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(9657..11441)
                     /locus_tag="NBL02_001453"
     CDS             complement(9657..11441)
                     /locus_tag="NBL02_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2572143.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11456..13177)
                     /locus_tag="NBL02_001454"
     CDS             complement(11456..13177)
                     /locus_tag="NBL02_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2572144.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13277..14038
                     /locus_tag="NBL02_001455"
     CDS             13277..14038
                     /locus_tag="NBL02_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCD2572145.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4627 a         2661 c         2863 g         4150 t
ORIGIN      
        1 cggctggttc ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag
       61 tttggaacaa aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta
      121 gacgacaagg atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt
      181 gcgtgtagtc tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt
      241 gaaaatccct gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc
      301 ttgtagaaac aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc
      361 atcgattgct tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc
      421 tgctagaaaa accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg
      481 ttgcaagatt tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg
      541 ctcacctttt tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca
      601 tcaattgcat tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc
      661 cacggcgcat cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg
      721 tcgcaaatga cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag
      781 tcccgcaaca ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata
      841 tctgaatcta aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt
      901 aatccgttga tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca
      961 tctaaataag tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt
     1021 gtttgcaatc caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca
     1081 actacttcat ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt
     1141 aaattgacta cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta
     1201 aaattttgga aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg
     1261 atttctttct ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa
     1321 ccagatacac tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag
     1381 tcaaatcctc catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg
     1441 taattccaag aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct
     1501 tctttctgta agagttatct atttgttttc ttttttctct atacactgga taagtcaggc
     1561 acagccaaga aagactgagt aagtagccgc cgatgatatc agtaggaaaa tgaacgccca
     1621 gataaatgcg gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg
     1681 tgcgtaaggt tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa
     1741 tcatactgcc tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg
     1801 ccaatgaagg acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa
     1861 ttacaataaa tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc
     1921 ataaagccgc acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa
     1981 agagttggta gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat
     2041 cgaaaccaga aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga
     2101 caagaaagaa gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt
     2161 gaagcaatga cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac
     2221 caatgtatga ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga
     2281 aagacatttt ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa
     2341 tccttttaaa caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt
     2401 acggtaaatt acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat
     2461 attaagagaa gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga
     2521 aaacattgca agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa
     2581 acgaggggca gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt
     2641 acagccggtt cctgttcaag cggatgaaac acctgttcag ttcggcgcaa gaatcaatac
     2701 agcggcattt atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct
     2761 atatgcatca gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt
     2821 atctcaagca ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt
     2881 ctacatggat acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg
     2941 acaatatcct tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc
     3001 atttggcaac ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga
     3061 tgcgacagct tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa
     3121 taatatcatc gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg
     3181 cggtggagcg actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag
     3241 caattctggt aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg
     3301 tgattctgtt tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg
     3361 gaacaacatc caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca
     3421 ggaaaatggc tcttcgacga ataattccgg taacaatacc aactcttcag gaaatgctgg
     3481 tacatcaaac ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt
     3541 ttggaagatc tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat
     3601 caagaacaat ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa
     3661 tgctggagca aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc
     3721 aagttcttct aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg
     3781 gagcgtctca aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca
     3841 aaataacttt atctacccag gacaacaact gactgtttca aacagtggat caacaaacag
     3901 cacgtcaggt aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg
     3961 cagcagttcg tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc
     4021 aaataaaaac ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt
     4081 catttatcca ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg
     4141 ttcaactact tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc
     4201 atccactggt aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa
     4261 caaacatcat atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat
     4321 ctatccagga cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc
     4381 agttactggt tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga
     4441 cagtcatgga attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat
     4501 ttatccaggt caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt
     4561 gaattccaca aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg
     4621 aagtagtaaa aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc
     4681 ttttgaactc gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg
     4741 gccgttacaa cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta
     4801 cgtcctataa aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag
     4861 aaaacaaagc aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat
     4921 aatagaaaca agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga
     4981 aattgagaaa aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga
     5041 tcctgaaaag ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct
     5101 acatgtaggg catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag
     5161 tcaaggatac aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca
     5221 atatgcgcta gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt
     5281 ccgacgccaa atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac
     5341 tgatccggag tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct
     5401 agcttatgaa gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa
     5461 tgaagaagtg atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat
     5521 gcgccaatgg atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt
     5581 agttgattgg ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg
     5641 agcaaatatt gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg
     5701 accagatact ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag
     5761 agaaattacg acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa
     5821 aaaatcagat ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc
     5881 ttatgcaatc aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt
     5941 atcttcatac ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga
     6001 attcgctcaa actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa
     6061 agctgcttac acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa
     6121 acaagaagca atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga
     6181 agtcagctat cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat
     6241 cccaatcatc cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt
     6301 acggttgcct aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa
     6361 tatcgaggag tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac
     6421 gaatacaatg ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca
     6481 taacaaaaaa gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta
     6541 cattggtgga gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt
     6601 cttatacgat atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg
     6661 aatgatctta ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc
     6721 tgatgatgtg gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg
     6781 gccattagat gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt
     6841 ggatcgtgta tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac
     6901 attgaatgat ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga
     6961 ctatgaaaat cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc
     7021 ctataaagtg gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc
     7081 gattgctcct cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc
     7141 ttacgcacca tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt
     7201 tttccaagta aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga
     7261 tgagctagaa aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac
     7321 agtcagaaaa gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc
     7381 tctttgtcaa taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca
     7441 ttctgtctca gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga
     7501 ttttcatgtt ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg taagagatct
     7561 ctgtaatcat caaatttttg tctattaatc ttagtatttt ttgatgtata aactacctga
     7621 aattgagagt acataccaaa ttcataaatt ttttattagt gggactagtg attgtatgga
     7681 ttttgacgca taatgaatga agtatgagaa aaggagagac aaatggatca agaactattt
     7741 attatcgaag tattatcagc taaacaaaaa attatcgaga aagcgaaaaa aagagcgaaa
     7801 agaataatgt tcaagctttt aagcttagta tttttctctg gtttgctttt tttcggagct
     7861 gtatcgttcc tatatcgcca tatgagtgca gtagtccgtt cagctgaagg gatgcaaaaa
     7921 ttaaataatt tgtatcactt ttcgtgggga gttggcggaa taacaattgt tgtgcttatt
     7981 gtttttcttc tacttatttg gctccgttac catcaattgt cgcgtcattt cgatcaatgg
     8041 cttagtcaaa gagcacaaca gtttttacat aataaaccaa cttctcaaga tgatgactat
     8101 ctttattttg agaagacttc gacgagtcaa gcttttcgga tacaaaaaag tgattgccag
     8161 ttattgctga gatcacttga tgatttacct atctacttag gcgaaagcaa atcaaaattc
     8221 ggaatgaaca cattccatat ttttttacta gaaaacgaac ccagatggga aaaaagccag
     8281 tcttcgcctg gtggatacct gacaactaag atgagtacat tagtcactgg ttttttcctc
     8341 attcttttag ctggaacaat ctattatggg agatacactc agccaaaaat gtcttctcat
     8401 ataaatgctg cttctcacat gacaatgaac tcaactaacg atacgcatat acaaaagaca
     8461 aatgaagggt tgttagctga tcctaagcaa aaggtaaatt tgacaacaca aaagataaat
     8521 gatctcaaac ttgatacaga gacgcactct ctctatatga caacagatag tggtgctaat
     8581 tgggtatttg tgccgattga ttcgcaatgg ttgcgttttg gcgaatacac cctgacaacg
     8641 gggctgattc ctgaaggtta ttggatggac aacacatatg atatttccgc cgatttctca
     8701 tggtttatct attcttctgg ggatgatgta cgtctgctga cttcaagaga taatggtaaa
     8761 acatggcaaa cgtctattat cacccaatca gttcagccga tacgttatcg aaaagcggta
     8821 ttttatggca acgatggtct atttatctgt acatcagcca gtgggatgtc tgcagaaact
     8881 ttgtacattt acactactat ggatggcgga atcacttgga taaaaagtgg aagtacaatg
     8941 atcaatcagc cagtacaaaa tgtcagcttc gttacaactt ctttaggttt tgtttcgaca
     9001 cgtgaaagtt tgttttatac aaacaacgga ggacgctcat tcaaagaatc agttctgtcg
     9061 attccggaaa actatcagac aggggggata gatctttttc aatcaccaaa tgaagttact
     9121 caaataagcg cgaatacgtt agagacaaaa ttttatttgt tgaaaaaagg accaatcgat
     9181 gcagggaaaa tgtttgcctg cctcttccgt tcaactgata acggggaaac atggcaattc
     9241 gttcagcaac tatcgcaagt agaacaagga gattaaagaa gagagattgt gagcctgacg
     9301 ttcagcaccg agtcacaaga aacaaattca aaaaacagct tctcatattt gttgaatagt
     9361 gtaactcgtg attcggagtt gacaagagca acaagaagcg attttgaagt atagcttctc
     9421 gtattttaca agatcgtgtc ttattgccga taggttgtct cttgaacacc gtttattcgt
     9481 aaaaagggat tgtgacaagt cttttgtcac tacttaccga acgaaactga aggagctatc
     9541 caaaaagtat cattagtatt attctttgtg tttctagtgg caccactagt acatttaaaa
     9601 aagctatcga tcatggacta atcatacatg atcgatagct ttttagataa tgtttgttac
     9661 gctatctgaa attgactatt ataaagactg gcataaagcg ttggtgattg gagtagttca
     9721 gaatgtgttc ctttttcgac gatatcgcca ttcttcatta caaggatcat gtctgcattt
     9781 tcaatcgttg acaaacggtg tgcaatcaca aaacttgtcc tatcttttgt cacagcttcc
     9841 attgcttgct gaatcaatgc ttctgttcgt gtatctacgc tagaagttgc ttcatctaaa
     9901 ataacgacag gaggattcgc tagaatgact cgagcaattg tcaataattg ttgctgtcct
     9961 tgagataacg tgccgttttc gctagaaatg atcgtatcat atccgtctgg cagtgttcga
    10021 ataaagtgat cacattgtgc gatttcagcc gcttcgataa tttgttctct tgtggcatcc
    10081 attttcccgt aggcgatatt atcagctacc gttccttcaa acagccaggt attttgtaaa
    10141 accatcccaa aagaacgacg tagttctcca cgtgaaagct tggtaatatc aagaccgtcg
    10201 aattcgatct ttcctttatt cagttcataa aaacgcatca gcagattgac taaagtcgtt
    10261 tttcctgcac cagttggacc aacgatcgct accgtttgtt ttggctttac tgaaaaatcg
    10321 acttgattca tcaatatttt atctgatgtg taaccaaact gtacattttt gaaagctatt
    10381 tttcctttcg gttgttctaa aagtcttgca tctggaaggt ctggaatttc atctgtttca
    10441 tctaatatct caaatatcct atcgattgct gccattgcgg attggatcga gttgatcaca
    10501 taagaagcgg tagaaattgg ttcagaaatc tgattgatgt actgaagata agcttgtaag
    10561 aagccgatcg tcacaccacc tgatagaaca agcatagcac cgaataccgc acttatgatg
    10621 aaggcgagtt gattaatgaa acgaatggcc ggatagatag cacagttcat aaattgagca
    10681 cgtttaaaag cgttgtattg ctttttattt acttcgtgta tagcagcttc tgcatgagct
    10741 tgctgattga atgttttgac cactagatta ccagctaaga tttcttccat tcgattattt
    10801 aaaacaccca gttcatactg attctgttca gaaatctgtt tgtttttgtt cgcaatctta
    10861 gttgtaacaa ttgaactgcc aacgatcagc aataagacga gcaaagtaag cttgctttcg
    10921 atataaaaca gcatcagacc agcagcagct attgttacga cagaagtaaa aaattgattg
    10981 atacctgtca ataacacttg agaaagttga ttgagaccgg tagttgtccg actaatgatg
    11041 tcacctactt gatgattgtc aaaaaacgac atcggtaatg ttttgaattt ttttgtgatt
    11101 tcttttcgca ccctcaatgt gatcttttcg cttaacgctg ccattgtccg ttcctgaata
    11161 aatgaagtga tgctgcttaa gactgaacag ccgatcagca ataagacagg gaacaacaat
    11221 gcttgtttga tatcactgaa agaaagctgt ccaatacctg tttttcttat ttctgataag
    11281 agatcatcga tcccgattcc catgatcaag ggcatggcta cgactaatag gttcccgaat
    11341 aaactgcata gaaacaatcc gtaaaaatat ttatgttcag gtttgatcat tttccaaaaa
    11401 cgtatcaaag cacgcgtgtt caacgttttt ttcttttcca tcgtatccct tctttctata
    11461 ttccttgtga ttttgcaaac tcctgataag tttggcaatt agacagtagt tcttcatgag
    11521 taccttgtcc aacgattttt ccttcatcta aaacaatgat gttatctgct tgttggattg
    11581 tactgaggcg ttgagcaaca atcacgagag ttttctccga catgtgttta tgaagggctg
    11641 ctcttaatgc tgcatctgtt ttatagtcta aagcggagaa actatcatcg aatacataga
    11701 tctctgctgg tttgatcaac gctcgagcaa tacatatccg ctgtttctgg ccccctgaat
    11761 aattagaacc gccttgagca acaaagctgt ccaatccgtc aggaagggtg tccactagtt
    11821 cttctaattg agcgatcttg atcgctttag acaactcttc tgttgaggca tattcatttc
    11881 ccatctgcaa gttgcttaaa attgtcccgc tgaataagaa agccttctga gggacataac
    11941 tgatgtggga gcggatctgt tcttgagtca tttgagtaag aggaatacca ccaagggtta
    12001 cttttcctat cgtagggtca ctaagtctca agagaagttt cgccaaacta ctttttccag
    12061 atccggtccc gccaacgatc gctgttgttt ttcctttagg tattgtgaaa tcaatatctt
    12121 ctagaactgg atcagagatt tcagtatatt taaatgtgac atgttcaaaa gcagctgcat
    12181 ttgtctgatc aaaggttggt tcagctactg ttttgatcgt ctcttcctca atttcgatct
    12241 ctgcttgcag tatttcttct atccgtcgta atgaggcaaa cgatttaggg ataatgacca
    12301 ataccatagc tgcgatcatc aagtaagcaa gtgtcagcat agagtattcg actacagcag
    12361 tcacgacacc gatttgtaat gctccttctg ctacaagatt tccgccaaac cacaaaatag
    12421 aagcataaac gattcccatg agcaaaaaag caattggagt gacccaagca aatattcggt
    12481 tcactttgat catttgatta gcatagtcag caaagctctt attcgttcgc ttttcttcat
    12541 atgattgatt atcaaaggca cggatcatat taatcccagt aaagaattct ctcaatgtga
    12601 tggtgatccg atccattttt ggctggatac tcaaagataa aggtgcccct cgtttcatca
    12661 gtcgataaac aatccataca taaaaaacaa cagagatcaa tgtaacccat gttagtaata
    12721 gagaataacg aaacatcatg agaatggtga aaacgcaaat aaccggtgct ggtaagatca
    12781 tttggcaaaa taaaacaatc atttgctgga cattatcgac atcattcgtc attcttgtca
    12841 aaagagatcc agaactaaaa cgactgatat ccttcattga cagctgttgg aatttacgga
    12901 aatatttctc ccgcgtttgg aatccaaacc tagcggaagc caatgctgaa aagtaactag
    12961 aggcaactgc agcgattgtt cctagaaacg ctacaagcag catttcaact ccaatttttt
    13021 ttataactga ggcttcgcca cttgctatac ctgtgtcgat cagctttgct actaattttg
    13081 gtacacctaa tgtaccggcg atttctaaac agatcatcac aaaggtaagg agagcgagtc
    13141 ctttgttttt ctgtaagaat gtttttaaga tagacatatt attcctcctt ttttcctgat
    13201 acttttcgat tctatacctt caagtaactt gaaggtcaat agaaaaaaga ataaactgaa
    13261 aaagaggtga gaaaaaatga aaaaaatgta tacgattggc caagtatcgg aactatttga
    13321 tttgccaaaa tcaacgatcc gctattggga tgaacaagga ttaatccgtt caagtagaca
    13381 agaagagaat gattatcgat tgtttgatat cgatgatatt tttatgcttt atgatatcgt
    13441 tttttataga aaattagata taccgatcaa acagatgaag aatttatatg gaaaaacatt
    13501 gacggagtta tatgagacac ttgatgaaac agaaagaaga atccataaag aactggtggt
    13561 gatgaaacaa aaacaaaaag agataaggga gcaaaaaaaa cagttgaaat tgatgatcga
    13621 tacaaacgag gaagaatttc ctgtagaaga gatccctttt gactgtatga tctcaacgga
    13681 gtttgaagat attgtggaaa tcaaaaaatt tctacctaac tattcaagct ttgggatgat
    13741 gagtatgcca gcttccagtt cacagacgat gtatggattt tttatcgacc ctagtgaagt
    13801 acatttattt actcaagacg tgatttggga gaaaaaagat acggctgttt atcgtcgatt
    13861 cttattgaaa tccgagatga accatgcgga acgtaataat attttagaaa tcagagaaag
    13921 aatgtatgaa aaaggatgga aaacaggaga agtgatcggc cagtatctat tgacgaatac
    13981 ggatgaaaac aatatcagaa ctgaatatta tcatgcatgg atagaaatga aaaaatagga
    14041 aatatgtgtt taagataact cgaaaatgat ggttgtttta gttataaaga aaaattttct
    14101 ggttcgatta cgggtttaat gtgggggatt attagtcgtg gatagataga attttagttt
    14161 tttagaggaa atatgtcttt aaaaatggaa tggttgcaat tcttagcgat atatacgatg
    14221 taattgtcct cttaaaaaag tatggaaaaa taattgtatt gggtgttatg tataattcat
    14281 ggtgaactga gactaatgag a
//
LOCUS       DAHHZJ010000060        14186 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.061, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000060 DAHHZJ010000000
VERSION     DAHHZJ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.061"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(<1..327)
                     /locus_tag="NBL02_001456"
     CDS             complement(<1..327)
                     /locus_tag="NBL02_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HCD2572146.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            504..1232
                     /gene="sfsA"
                     /locus_tag="NBL02_001457"
     CDS             504..1232
                     /gene="sfsA"
                     /locus_tag="NBL02_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCD2572147.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            1248..2261
                     /locus_tag="NBL02_001458"
     CDS             1248..2261
                     /locus_tag="NBL02_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCD2572148.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            2261..3538
                     /locus_tag="NBL02_001459"
     CDS             2261..3538
                     /locus_tag="NBL02_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCD2572149.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            3601..6303
                     /locus_tag="NBL02_001460"
     CDS             3601..6303
                     /locus_tag="NBL02_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCD2572150.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            6455..6772
                     /locus_tag="NBL02_001461"
     CDS             6455..6772
                     /locus_tag="NBL02_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCD2572151.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            6802..7122
                     /locus_tag="NBL02_001462"
     CDS             6802..7122
                     /locus_tag="NBL02_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCD2572152.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            7230..8690
                     /locus_tag="NBL02_001463"
     CDS             7230..8690
                     /locus_tag="NBL02_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCD2572153.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            8758..8979
                     /locus_tag="NBL02_001464"
     CDS             8758..8979
                     /locus_tag="NBL02_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572154.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            9010..9192
                     /locus_tag="NBL02_001465"
     CDS             9010..9192
                     /locus_tag="NBL02_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HCD2572155.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            9192..9605
                     /locus_tag="NBL02_001466"
     CDS             9192..9605
                     /locus_tag="NBL02_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HCD2572156.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            9728..10909
                     /locus_tag="NBL02_001467"
     CDS             9728..10909
                     /locus_tag="NBL02_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD2572157.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(10980..11144)
                     /locus_tag="NBL02_001468"
     CDS             complement(10980..11144)
                     /locus_tag="NBL02_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572158.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            11298..11371
                     /locus_tag="NBL02_001469"
     tRNA            11298..11371
                     /locus_tag="NBL02_001469"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11332..11334,aa:Arg,seq:cct)
     gene            complement(11440..12579)
                     /locus_tag="NBL02_001470"
     CDS             complement(11440..12579)
                     /locus_tag="NBL02_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCD2572159.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEVK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(12693..13277)
                     /locus_tag="NBL02_001471"
     CDS             complement(12693..13277)
                     /locus_tag="NBL02_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HCD2572160.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(13404..13835)
                     /locus_tag="NBL02_001472"
     CDS             complement(13404..13835)
                     /locus_tag="NBL02_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCD2572161.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(13853..14173)
                     /locus_tag="NBL02_001473"
     CDS             complement(13853..14173)
                     /locus_tag="NBL02_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD2572162.1"
                     /translation="MITFERIKELAKKQGKSLNKVEEDLGYGKNVLYRLKNSNPSTER
                     LQEIADYFDVSVDYLLGREEKETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
BASE COUNT         4545 a         2422 c         2820 g         4399 t
ORIGIN      
        1 tttttcagat aacaacttat atgaagtgtc tgcagattcg tcataaagag tttgttcggg
       61 ataagaaaag ataagataaa aaccatactg atcacgtacg actaaacgat attcatctat
      121 ttctaccaca gatgtgccaa gagactgacc gttgatcaaa aacgtgtaat cagatgtaat
      181 ctgtaattgt tgtttttgct ggctatcact aaagaaccat gtaccttgat actttgagag
      241 tccttctgac ttttccgctt gttttttctt tttcaagtaa ataaaacttg agagaacggt
      301 aatgagtgaa ccaagaaaat agatcatttt ttttatcacc aaatcactcc tttatgagaa
      361 aataatcgca tagacatatt ctttcttctt tatttcattg taccgaataa aagctaaaat
      421 ttctctaaga ctttcgactg gtttttttac tgtttttcta caaattatga taggatacat
      481 agtaggaaga aaggtgacat aatttgaagt atcagcaagt ggaaatagct ttttttattg
      541 accgcccgaa tcggtttata gcacattgta tgaaaaaaga cggagaaata attattgcgc
      601 atgtcaaaaa tacaggtaga ggaaaagagg tcctgatacc aggcgctgaa gtggctattg
      661 tatttgctcc tggacctaaa cggaaaaccg cctatgatct gattgctgta aaaaagaagg
      721 gtgactggtt taatatcgat agtcaattgc ctaatcggct ggcaattgat ggtattttag
      781 acggcacgat ccttttacca ggattaactg gtccatttac ggtatacaag cgtgaagtaa
      841 cttatcttca ctccaaattt gatatttatt tagagacggt ggtcggacaa aaagcatttg
      901 ttgaagtcaa aggtatgaca cttgaaaata aagcgattgg tgcttttcct gatgcgccca
      961 cactcagagg attgaaacat ataggagaat taacgtatgc cgcacaagat ggttatgccg
     1021 cttatgtctt gtttattgct cagttcgaac atcttcatct ggcaacgatc catgaagaaa
     1081 tgcaaccagc actagctgat atggttcgac atgcgcagca aagcggagtt caaatcctcg
     1141 cctataattg tcaagtatcg gcagatgagg taactgtcaa gcaggccatt ccatttgatg
     1201 tagatatccc ttttgaagat cctatacaat agaaagaagg taaaataatg atcaacttag
     1261 gtattatcgg aacaaattgg attactcatc aatttgtcca agcagcttta agcacaaaaa
     1321 aatatgaatt gacagcggta tattctagac atttggaaaa agcacaggaa tttggttctc
     1381 attatgaagg ggatatcgaa ttcgcaacag atttgaagac ttttttcaat attactcata
     1441 tggacaccat ttatattgct tctccgaata gtcttcactt tagtcaagct aagcaagcaa
     1501 ttttggcagg aaaaaatgtg atagttgaaa agccagcttt ttcgacgcca aaagaaatgg
     1561 atgaaatcat ccatttggca aatgaacaca atgtgtattt ttttgaagca gcaagaaata
     1621 tccatgaatc aggttttaaa aagattgccg attacttacc attaaaagac gagataatcg
     1681 gtgcaaattt cagctatatg aaatattctt cgcgctatga ccaagtatta gatggagaag
     1741 aaccgaatat cttctcgact catttctcag gtggtgcatt gatggattta ggtgtttacc
     1801 ttgtatatgc agctgttgct tggtttggaa tgccaaaaga agtccattat ttcccaagaa
     1861 agatttctac tggtgtagat ggtcttggat gggggatctt acgttatgag aattttgatg
     1921 tatcgatcca gccaggtaaa atcggtgatt cttatttacc gtcagagatt tattttgact
     1981 caggaacgct agtgatgaat ggagtcaatg caattgaaca agcacaattt catgatcgcg
     2041 cgcataaaga aatcattgat ttggcggtaa cagctgcaga aaatccaatg gcagatgaag
     2101 cagaggattt tgccaatgtc atggctcatc cgaaagatcc agcttgggga ttgttgtatg
     2161 aagagtggat cgagctagct agaaatgtga accaagtgat ttatgattta agaaaaaatg
     2221 gcggcatcaa attcgatgca gataatgagg aagattttta atgaacgaat ttgtacaagt
     2281 atttcccgaa aattggcaga aaaaatatga ggataaaggg tttatctcac cttctcttat
     2341 tcaacgagca gtttttcagc cactaagtaa taaacaaagt atagtagcgg tttctcctac
     2401 aggttcaggt aaaacactgg cttatctttg gcctctacta ctaaacgtgg aaccaggtga
     2461 agcaagtgcg ttggtcattt ttgcttcttc tcaagaacta gctattcaag tagcagatgt
     2521 tgcacgtgaa tggggaaaag acaaagagtt gaaagtccaa tctttggtgg gcggagccaa
     2581 cgtcaaacgt cagattgagg gtttgaagga aaaaccagaa atcttgattg gtacaccagg
     2641 aagaatattg gaattgatga aagcaaaaaa aataaaagca catcaagtga aaacaatggt
     2701 atttgatgaa gcagaccagc tatttgacgc tgggaacagc caaatcattg atcaaattct
     2761 tcatcaagct ccaacagagt atcagctagc ttttttttca gcgacagcag atcgttccct
     2821 tgaaagcatt gaaaaaatca ctgggaaaac gtttgagaca attgacgtga cagcagagga
     2881 cgattctcga aaaggactga gacattactt tttacgtgta ccgatacgaa agaaagatga
     2941 ctacttgaga cgtctcacgc atatagaaga ttttcatggt ctgattttct ttaatcaact
     3001 gactgaatta ggcgtaatgg aagaaaaatt gctgtataga ggtgtaccgg taggaagtct
     3061 ggcctctgat cagaacaagc tactccgaaa agcagcgcta gaacaattca aaaagaaaat
     3121 ggtcagagca cttttgacca cagatgtggc ggctagaggt ttagatatta caggtcttcc
     3181 ttatgtagtg aatgcagaag ttccgttaag tgaagaagct tatttgcatc gttcgggaag
     3241 aactggtcga atgggtaatg aaggaatagt aattacactc gtacaagata ataatctaag
     3301 agatttaaaa cggcttttac gtccatcaaa tataacactt gaagagattt ttttatacgg
     3361 aggtaagctg caaactgagc agccaaccaa agaagatgca gctcctgcag taaataaaaa
     3421 gtatgcgaaa actttttctg gaaaggagca gaacccagaa tataacaaag aaaaaataag
     3481 aaaaaaagtt aaaaataaat caaaaaaaga gagaaataaa ggagcaaggc gcaaataagc
     3541 gcctttttat gctatgatta tgaaaacggg aacaaaattg gttgccagag gaggggttat
     3601 gtgaaagaac gtcaagaaga gatcttgcag ctattgacag aaaaccaaca aggattgaca
     3661 gcaagtgatg tagctgagcg cttaactatc gatcgaagca atgcaagccg atatttaagt
     3721 gaactataca aggcccatca tattgtaaaa accgctggcc gaccagtcgt ttactctttg
     3781 ccaacggaaa agtcaaaatc tgatgaagtg catgtcgact cttctacgca agtgactttt
     3841 gaaacactcg ttggagaaaa tgattcttta aaagtcagca tacagcaggc aaaagctgcg
     3901 attctttatc caccgcgggg gttgcatacg attatttttg gagaaactgg tacagggaag
     3961 tcgatgtttg ctgaatgtat gtatcatttt gcaatcgatt cagagatgct ttctgcagat
     4021 gcgccatttg tttctttcaa ttgtgcggat tatgctcaaa atccgcaact gctctttgga
     4081 cacatctttg ggatcaaaaa aggggcttac acaggagcag ctcaagatag tcccggtcta
     4141 atagccaaag cagacggcgg tatattgttt ttagatgaga tccaccgttt accgccggaa
     4201 gggcaggaaa tgctgtttac tttcattgat aaaggtattt accgtccgct tggagaaagt
     4261 gcacaagtac atgaagcatc tgttcagatc attggggcga cgactgaatc ttcagaaagt
     4321 ttcttatcta cgttcaatcg acggattcct atggcaatca ccttgccgaa tcttgcttca
     4381 cgttcactgg atgagcgtta tgaaatcatc tctcttttta tcaaacaaga agcgaatcgt
     4441 ttgaatcaac gtatcgatat cgaaaaagaa gcgattcttg ccttcatgct ttatgatgcg
     4501 gaaggaaata tcggtcaagt gaaacgagac ttgaaactag tctgtgcaaa atctttttta
     4561 cattaccgta ctcatcatga gaaaaagcta atcattcgaa aagaagaact ttcccttcaa
     4621 gtacaaaaag gattactgaa aatcaaggaa ataccggaac gtctcgaccg attcatggac
     4681 agcaaaagtc agtatttgac atttgaacca ggatttgctg atgttgtctg gtcacaagat
     4741 ccagaacgaa acatgcaggt ctataacgat attgaggaaa aagtcacttc tctttccgaa
     4801 accggtgtgg agaacattga tctagagacc ctcatttcaa aagatgtgga tgcttatttc
     4861 cagacatatg tcaaagaatt gactaaaagt tcgattccca aagatctttt gccagatgaa
     4921 atctggcggt taaccaatca attgtatgat gtggctgaaa aacggttaga ccgtatttat
     4981 aatgaaaaag ctcgttttgc ttttgctttg catctgcaaa gtacattgga tcgtgtgaga
     5041 gaaggtcata tgatcgtcca cccagatttg aataacgtac ggaaaaattt taagagggaa
     5101 ttccaggtag caatcgatct ttcaacgatc attgaagaag aagaacaagt tgagattcct
     5161 tttgatgaaa tcggctttat tagtatgttt ctttctatca atctaggaga aagtgagcct
     5221 gttaaagaaa acaaagtaga tgttctcata ttgatgcacg gcagacagac tgcttcaagc
     5281 atgttggaga tggcacaaga actattagga acaaaagtag ggaaagcttt taatatgcca
     5341 ctggttatgg aagttcaaac aatgtatgaa gaagtgttac aatatgtcaa aaactatcaa
     5401 gagcatttga cgaatggcct actcttgtta acagatatgg gatcattaaa caccttcagt
     5461 gatttgattt atgaagagac tggcattcgg acgaaagcta tcacgatgac aagtaccatg
     5521 attgtgatag aagcgatacg gatggcaagt gttggaagaa gcttagaaga tatttatcaa
     5581 aatatccagc tttcctttga atcgatcgta cgtgatcagt tcaaagcgga aaagtctgaa
     5641 actttacagc gtaaaaaagc cgtaatcgtg acatgcttta ctggtgaagg agtagctgcc
     5701 aaattatatc agcgtattat gccagtgatt aatcaaaata aggtagaact gatccagatg
     5761 cagtttttag aacgtgaatc attcaaaaaa cacatcgatg ccttattgga agaatatgag
     5821 atcaaagcaa ttgcgggaac agttgatgtt gattatcaaa atatcccatt tttttcagcc
     5881 tatgacgttt ttgatgatga aaaactaaat attttaaagc gtatagctga tgatgagatc
     5941 ccgacagcta aaattgtcga ttcacttgct ggatcattaa gcgatgtctt ttctattgat
     6001 gaactgatcc gttcattgca aaagacagtt cagcagatcc agaccgattt gtcgattatc
     6061 gtggaacctg gagtagatac tggaatcgtt atccatttag ccttcatgtt agacggattg
     6121 atcaaacaag aaagccatcg gcaatttgag aaactgtcta ttttctcaaa aatttatcgt
     6181 ttggaaatgg atatcgttcg ggcaaacttg atggcactag aacgaaaata tcaggtaaaa
     6241 attccagaag atgatatcgc atttcttaca caaatgtttc ttgaaaataa aataaaaaaa
     6301 tgaacacact aatcatactg tttacacact aaacatctgt gtgcgcacta taaaaaagag
     6361 ctaaaaagca tgaaaacgct atttcagctc ttttttttat tggcatgctt cttgcgttat
     6421 ataaaagtgt aaaaaaataa aaggagtgtt aaaaatggca aaaaaaacaa tcatgttagt
     6481 ttgttcagca gggatgagta cgagtttatt ggtcacaaaa atgcaaaaag cagcagaaga
     6541 aaaagggatg gaagccgata tttttgcggt ctctgcctca gatgcagata ataatttaga
     6601 atctaagaat gtcgatgtat tattattagg tcctcaagta cgtttcatga aagctcaatt
     6661 tgaacaaaaa cttgcgccta aaggtattcc attagatgtc atcaatatgc aagactatgg
     6721 catgatgaac ggagaaaaag ttcttgcaca agcagaaaat ctaatgaaat aaatcaagta
     6781 tgcagttggg ggtataacaa cgtggaagat caacaaaatc tagaagccat catggggtta
     6841 atcatgtttg gcggtaatgc aaagagcgat gcaatggaag cgattgcggc tgctaaaatc
     6901 ggtgattttg agttagcgga taggaaaatc gctgatgcag aagaatcact tatacaagcg
     6961 catcatgcac aaacagacat gttgacacaa gaagcacaag gaaatcatat gcaagtgaca
     7021 ttattgactg ttcacagtca agatcactta atgacatcta ttgcgtttac tgatttagca
     7081 aaagaaatca tcgaacttta tcgtcgcgta tacagcgaat aaatacaact tcttttttga
     7141 acggaagaag aaaaattttt gaacggattt tttattttgt tataaggaga actctcttta
     7201 tataaaaaac ttataggagg aagtaaaaaa tgaacggact atcaagttgg atggagaaat
     7261 acatcctgcc ggttgccgca aaaatcggag cgcaaaaaca tttagttgct ttgcgtgatg
     7321 cttttatcgg cacactgccc gcaacaatgg cgggatcagt cgctgtaatg atcaatgcaa
     7381 ttattcgtga tttaccgcaa cagtttaaca gtacctatgc tgacagttta gcagcagaca
     7441 aaggaatctt tgccgtattg aatgtcatca tcggaatcaa tggttttgtc tggaacggta
     7501 cactagcaat cgccggattg atctttgcct tttcttgggg gtataatctt gcccgagctt
     7561 atggagtaaa tgatctagct ggggggatcg ttggtttagc taccttgatt caaggtattt
     7621 cctttggtcc agcattatca gcagcattag atgtgaatgt tccttcaaat gtcgcagatg
     7681 caatcaatag ttctgagatt ggtgcaacga ttgcagatgg cacactgtca gttggattat
     7741 ggggttggct aaaactagat catttgaatg gaaacgctta ttttacagtt atgatcatgg
     7801 gagctatttc agtcattatt ttttgtaaat tgatgcttgc caacattacg atcaaaatgc
     7861 ctgattcagt accaccagca gtatctaaag catttgcagc cattttacca gcgacgatcg
     7921 ctttatacgt cattgctatt atcaattata tcgtaggtcg tgtagctgat gggcaactga
     7981 tcattgattt ggtgcaaaaa tatattgcag aaccattcct aggattatct caagggttgg
     8041 gagcagtatt gatcgtcaca gtatttgttc aagtattctg gttctttggt attcatggac
     8101 caaatgtatt ggcaccagtg ctagagggaa tctggggaca agcacaacta atcaatattg
     8161 atattttcca aaaaggttat aacggaaaaa ctggtacacc agccgtgtta gatgcaatcg
     8221 aagatggaaa agcttatatg tgggtccgcg gatcgttcga tgcttttgca tggtttggcg
     8281 gatcaggcgg aacgatcgtc ttactgatag ctatcttgat tttctctaaa cgtgcagatt
     8341 atctaacagt agctaaatta tccattgggc ctggtatctt caatatcaac gaaccagtga
     8401 tgtttggtct gccaatcgta ttgaatgcaa tcatgtttat cccattcttg attgctccag
     8461 tagtagcaac agcaattgga tggacagcta cttatcttgg cctagtcgct ccggtatcgc
     8521 agcaagtcac atgggtagta ccgccgttct tattgtcatt cttagcaaca ggagcggatt
     8581 ggcgggcacc gatcgtaaca ttagtatgta tggtcgtgac tttcttgatt tgggcaccat
     8641 ttgtcatctc tgcaaacaaa atggaccctg ccgcaggcga acaacaataa gatgaacgtg
     8701 aggagcggga cgctttaagt ttcgttcctc ataatgaatt ttcgaggagg aactgaaatg
     8761 attacaattg ccagtgcaat gcaacaagat gaaatcaaag aattgttaga aggatacaat
     8821 gaagaaggaa tgacttttac atttaaagaa aaacaaggaa tcaagctgtt ctttgaaaca
     8881 agtgcagaag atccggaagt tgcggctaaa aaagcaaagg aactaatcaa agcacagcct
     8941 tggggaacag tcctttattt tcaatcaaca gctgtttaga aaatggttta aaaagatagg
     9001 atggttgaga tgataaaaaa cggtttattt ttatgtagta ttggttcttt gatcgtgttg
     9061 tatagtttga atccacattc gcaaaggtat gatttgtata gtatggtcac aggtcttttt
     9121 ctaacaggat taggaatcta tttcttcatg aaggggaaaa aaagagaaga agcaaaggag
     9181 aaaaataact aatggaaatc acgaaaaaat taaatattcc agcatcgttt ttttacgata
     9241 aagttatgga ctctgtcctt ttcgatgtac gtaaagcgac cggcaaatca gtaactagaa
     9301 aacagctgaa aaatttggag tacgtcaaac aattctcgca aaataaccgg gctcgcataa
     9361 aaatcgaaga agtcattgaa aaccagtctt ataagtttcg tacatcgaca acgaagaatg
     9421 aattcgtcgt tcattatcaa atcaaaccat tggacgattc gacctgtgag gttcattaca
     9481 cagaaaaaat ggagtcttat ggcatgcttc aaaagttgaa tgatatgctt ttaggaacaa
     9541 tcttgatgta ttttaaacgg agacgtttca aaaaaatgct agatatgatc gaagcaacgt
     9601 attaaatcaa tgaaacatgg tactatttag ttattgaaaa atgaagtaag ctagaaggaa
     9661 tttttcatag gaagatagat ctttcctgcg aatcctcact agcttttttg taggaagggg
     9721 cagcaaaatg acaaacaaag taagagagct aggtgtatct tcgattgagg aaatgtttga
     9781 tttagccact tatgcattta atggggcaga tacacctgaa aggcgagaac gttttcgtac
     9841 acttgcggag aattcttgga attacggatt ttttgatgag gaggatcggc taacaagtca
     9901 agtcatggcg accccttttc cagtgaattt ttacggacaa gagtacctaa tggccggcat
     9961 tggctatgtt gcttcttatc cagaagcaag gggacaaggc gggatcaatc agatcatgga
    10021 aaagatcctt gaagattgcc gtgatcgtaa agtaagctta tcttatctgg cgccattctc
    10081 ctatcccttc tatcggcgct atggctatga acaatcattt gataaaatca gttatcaact
    10141 aaacagtcgt gatgtgccgt ttatcaaaaa aaaatctgga aaaataaagc gtatggattt
    10201 tgaagatgca aaagctgcaa tcagaagaat ttatgaacag atgccggaaa accaacgagc
    10261 tggtctgaag cgcgaagact ggtggtatac gtataaattc aagcaaaaag gcaattacca
    10321 gtttgctttg cattttgatg ataatgagga agcaacggga tatatcgttt atcagcttgc
    10381 aacgtcttcc tttattattg cggagtgggg atttttggat catgatgctt tttgcagcct
    10441 tgttcgcttc gtaagttctc acaatggtgc ttttgaaaca tttgaatatt catgcggcta
    10501 cggcggtaat gatcaaaatt atctattaga aaatccattt gcttctgtaa tgatcacacc
    10561 ttatatgatg gcgaggatcg tagatatttc tttgtttttg gaaaattatc catttaaaaa
    10621 gagacaagct gcatttgccc tacaaataga agaagatcat tatgcgaaat ggaatgaagg
    10681 aatcgtggaa gtaacaatcg aaaatggaaa aaatcatctc tcaaaagtag aacaaacaaa
    10741 actcccaatc gttcgaggaa gtatccaaca tatcacgcag ctattattag ggtatcgtaa
    10801 agtggaagag ttggtattcc aagaaagaat acaggtgaca aaagaatatg aagaaacgtt
    10861 aagctcaatc ttgccacagc aacagccaat tcttaatgac tatttttgat aaaaaacggg
    10921 tgatgacaat atttttgtca ccaccctttt gtttttcatt tggcatttga aggaaaatgt
    10981 taaagtaaat cctctttttt cgtaaatttg aattttttgt tttcttttgt tttagggttt
    11041 tcttttttgt ggtcttggat tgcttctttc gtaatctttt cccgattttt tttagatgaa
    11101 tctaagcgtt ctgcgtatac tttttctttt ggcttatcat tcattggaaa ggacctccct
    11161 acattatttc attctacctc tagtttatca tctgttaagg actagtggaa acgttttgcc
    11221 taacttataa gctgattttt ttagcagata agcttgcaag taaaaaacaa acaagctaca
    11281 attgatttat tgaaattgtt cctgtagctc agtaggatag agcaatcgcc tcctaagcga
    11341 taggtcgctg gttcaattcc agtcaggaac gtacaaataa cccctgtatc ctttgtggta
    11401 caggggtttg ttttgtattt gtgccaaaaa tgtgccaaac tagttatcat ttttatataa
    11461 actatcgaac acagtattga ttttttcttt atcctgatct tcaagctctc gaacaatatg
    11521 aagataagta gacattgtag tttctaaact gctatgtcct aaacgtttcg atacgctaag
    11581 tatatttact ccttgataaa gtaaaataga tgcatgtgta tgccttagtc catgcaaagt
    11641 aaattgcttt tcaatcccta attcttttaa ttttttgcgc aaatatttgc tgacggcgtt
    11701 tgatgaaact aatccatttt ttaagttgaa aaaaacaaaa ttatgagggt ttttaacttc
    11761 aaaattctcg aataattctt tttgattgat tttaaacttt tttaagagat caatcgtatg
    11821 tttatcaatg gaaattttcc tatttgaagt ttcgtttttt gtttttcccc attcatttaa
    11881 tttataattc catgttttat taatagtgat gatttgatct tcgaaatcga tatcttccca
    11941 tgttaatcct agaagttcag caaatcgcat tccagtagca ccagcaacta acacgagcat
    12001 aggagaagag tactgcgcag aaaggttttc ttctacgagt ttcattaaag atttaaattg
    12061 atcataatct aaatatttat cttcttttgg cttcaatgaa tcttttcctt tgattactgc
    12121 ttttctagtt ggatcaaacg gaattaatcc ttcttctacg gcatctttca acgatgctct
    12181 aatatggtta ttaaacttaa taacggtgga ttttacatgg tctttcgcat acttatttaa
    12241 aaagcgttga tatccaactc tatctaaatc agaaatcaat accgctggca tatatttctt
    12301 tatgttcatt aacgtatctt cgtattttct ataagtgata ggagatactg ttccttcttt
    12361 ataaagttgc atccaatcct caaagtaatc tgataataat agatttttgc gatccccttt
    12421 aaggcctttg ttcaagttat attctaattc gttggcggca tcttttgctt cgccttttgt
    12481 cttaaatcct gattttctta tcttcgtgta ttttccgtca tcttttttgt atgaaatttc
    12541 gtattgccaa ctatttccac gtttgactaa tcttgccata attgaatcta ctctctttct
    12601 ctgatacaat aggcactata aagaagccta ctgtataggt ttgtttttct tagaacacgc
    12661 ttgctttgga cggtgggcgt gttttttatt tattatacag ttgctttaaa aatagctgct
    12721 ttttcaaatg atactaaagg agagaagtgg atttctattt ctcccttttg gtttaacgtg
    12781 aaatgagcag ttacatccat ttgttttcct ggagcaacag atcccattgt attttcgttg
    12841 gcatatgtct ctgatttttt gtcatctggt ccatatactt ccacatctgt acctacaggg
    12901 atatctgaat cgccatcatt ttttacggta taagtaattt ttactacttg tgcaggttgg
    12961 ttttcttcaa attgatttct ttcatcagtt agttctacac tatttagtgt atattcagca
    13021 tcaccaactt ttacagtgtc accaatctta tagaaggtat cgcttttttc ttctttagaa
    13081 gatgaagcag aggttgaaga tttagttact ttttcgccac cattatcact tgctttatta
    13141 ctatctgatc caccatttaa agcagaacca ataattataa ttaaaattac tgctaatacc
    13201 caaaaccata cttttttgta aaaaggttta ctaactttat acatttttcc gtcttgaccc
    13261 ataacttttt ttgccattta aatattcctc acttcttgtt ataatatatt tgcgatctca
    13321 gaaatgaggt atgagtccgt gtgccagcac gggctttttt aatgttttgc tgaaatcggt
    13381 ttttttaata attcttgatg tattcataca tattgccttg cgtaagaata ttttttctta
    13441 aaatagcatt ggcagacagc ataacaagtg tatctgagct tattctgaat agaataatat
    13501 tccataaatt tttcgaggtt aaactgagat tcatcagtca gttcgttctc aatatagata
    13561 tttaataaaa ttagaatagc tattttatct gcttctgttt caaattttga gtgaaaagtt
    13621 gtagaagtat cgtacaaaac tgaaaattca aaatgagagg cgctgaaatg tgcaagttca
    13681 tgagataaat gaaaggcttc tgcagtttcg ccatatagat tttcattcaa aaaaatgatt
    13741 ctaggcttcg gataatagaa gccaggctct ttcatttcca tgtaaactac tttcaaattg
    13801 tattcgctca acatttcttt caatttcaaa tacatacaac ccatcactcc aactattcat
    13861 tttcctctaa agctttagca attgcaatcg ctttacgcat tgtctcctta gatatttctt
    13921 ttccatcaaa agaaaaaaca gtatcgtttt ctgataaatc cacatgttta ggggtttctt
    13981 tttcttcgcg ccccagaaga tagtctacag agacatcgaa atagtcagca atttcttgca
    14041 aacgttctgt agaggggttt gagttcttaa gtctatataa gacgttttta ccatagccta
    14101 ggtcttcttc aactttattt aatgattttc cctgtttttt cgctaactct tttattctct
    14161 caaacgttat catatcaata ttcctt
//
LOCUS       DAHHZJ010000061        14065 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.062, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000061 DAHHZJ010000000
VERSION     DAHHZJ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.062"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(<1..779)
                     /locus_tag="NBL02_001474"
     CDS             complement(<1..779)
                     /locus_tag="NBL02_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCD2572163.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIV"
     gene            complement(752..1432)
                     /locus_tag="NBL02_001475"
     CDS             complement(752..1432)
                     /locus_tag="NBL02_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCD2572164.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1455..2393)
                     /locus_tag="NBL02_001476"
     CDS             complement(1455..2393)
                     /locus_tag="NBL02_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HCD2572165.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2638..3501)
                     /locus_tag="NBL02_001477"
     CDS             complement(2638..3501)
                     /locus_tag="NBL02_001477"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCD2572166.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(3643..6216)
                     /gene="mprF"
                     /locus_tag="NBL02_001478"
     CDS             complement(3643..6216)
                     /gene="mprF"
                     /locus_tag="NBL02_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HCD2572167.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6357..7163
                     /gene="proC"
                     /locus_tag="NBL02_001479"
     CDS             6357..7163
                     /gene="proC"
                     /locus_tag="NBL02_001479"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCD2572168.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7242..8798)
                     /gene="rny"
                     /locus_tag="NBL02_001480"
     CDS             complement(7242..8798)
                     /gene="rny"
                     /locus_tag="NBL02_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HCD2572169.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(9209..10258)
                     /gene="recA"
                     /locus_tag="NBL02_001481"
     CDS             complement(9209..10258)
                     /gene="recA"
                     /locus_tag="NBL02_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCD2572170.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(10367..11608)
                     /locus_tag="NBL02_001482"
     CDS             complement(10367..11608)
                     /locus_tag="NBL02_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HCD2572171.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(11848..12426)
                     /gene="pgsA"
                     /locus_tag="NBL02_001483"
     CDS             complement(11848..12426)
                     /gene="pgsA"
                     /locus_tag="NBL02_001483"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCD2572172.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(12635..13558)
                     /locus_tag="NBL02_001484"
     CDS             complement(12635..13558)
                     /locus_tag="NBL02_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD2572173.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
BASE COUNT         4029 a         3140 c         2461 g         4435 t
ORIGIN      
        1 tttacaatca ggaacaaagc aataaagact aatgtgattg ttgctcctgg aggtgtatct
       61 agataaaacg agctagttag tcctgacagc atccctagaa atcctacaca aacactgata
      121 gcaatcacga cattgaagct tttgcctaat cgcataccga ttgctgcggg aagaaccatg
      181 attgcggata tcaaaagtgc accggcaatc gggatcatca cagcaatagc cacaccagta
      241 atcacattga acatcatcga catcaaatga acgggtagcc catccacgtg ggcggtatct
      301 tcatcaaaag tcagaacata catcggccgt ttgaataaaa agaacaatat accgatggca
      361 acgaataaga ttcctaaaaa gatgacttgc tcccatgtaa tggtcacgat cgaaccaaac
      421 agataggatt ggatacttgt tgctgaattc cctccgctca aattcatcaa tactaacgct
      481 agcgccaaac caccagccat taaaatggca atggagattt ctgaataggt tcggtagatc
      541 gttcgcagat actccaatat cacagctgct aatacaacga ctagcatggt cgtaagtgta
      601 ggattaaggt tcaaaaagaa tcctaacgct acccctgcta aagagacatg agaaagcgta
      661 tcagccatca gcgattgtct tctgatcact aaaaacacac ctagcatcgg ggcgattccg
      721 gcaatgaaga tagcggcaag aaacgctctt ctcatgaatt catatgaaag caacgccatt
      781 gagaatcctc ctttctcacc agtcggatct gtctgtccgc atactgtttg atatcttcat
      841 gatcatgggt gatcattaga actgatttcc catggacatg cgcactatgt cggagaagac
      901 gataaaattc atttctggat tcttcatcca tacctgttgt tggttcatca agaacaaata
      961 agtcagggtc agtagcaaaa atacgcgcta aactgattcg ctgcttttgt ccgccagaca
     1021 attcacctat tcgccgattt cgcatatccc acatccctac agcttctaaa gcacgctgta
     1081 catgttgatg atctttttca gagagacgct tgaaccagcg gcctcttgga tatcttcctg
     1141 aacggactaa ttcgatcacc gtactaggaa aacctgcatt gaaagatgca acttgttgag
     1201 gaatataacc gatacttaat ttttttcctt cttgattttt ctcagcaatc tttatcattc
     1261 cttttgaagg tttcagtaat cctaacgatg ctttcaccaa ggttgacttg gctgctccat
     1321 tttctccagt taaaatgaca aattcacccg catctacatg ataagagaca tcttctagta
     1381 caggttcatc atcatagtaa aaggtcaaat cagatatttc gatataacgc accttacaac
     1441 ctccattcta aaacttaatt gatactttct tttaaagctt tcagattttt ttccatgaca
     1501 gaaatataat tttcgcctgc tttgatttgt tcattagtca agctttctaa aggattcaac
     1561 acttctagct tcactcctgt ttcattcgac aatgtctcag ctaccttcga agaggcattt
     1621 tcttcaaaat aaatcacttt gatgttgttt ttcttcacaa attcttttag ctcagctaag
     1681 cgacttggtg tgggttcctg atctggtgaa acgcccgaaa tcgctacttg ttttaagtcg
     1741 tattgattcg ctaaataagc aaaagcagca tgctgagtga caaatgtgcg gtttgttgca
     1801 tcttttagct catcggagta tttttgatcc agtgcttcaa gctttttgat ataagcatcg
     1861 gcattcttcg taaaagtttc ctttttatct ggatattttt cgcctaattc ttcagcaatc
     1921 gttctgactt cttttatagc taataccgga tcgagccaaa catgtggatc gtattcatgt
     1981 gaatgaccat gcccttcttc tgattcttca tgttcgtctg cttcttgcga ttctaaacgg
     2041 tcgattcctt tagctgcttc gatcatcaag gtctgtttgc tatccactga atcctttaac
     2101 gaatccacaa aaaattccat gtctgaacta ttatacacga aaacatctgc atcggaaatt
     2161 tctgctcttt cttttgcact tggttcgaaa tcatgcggct ccgttccagc agggatcaaa
     2221 agtttcacgt ttccttcatc ccctacgatc tcttttgtaa aatcatacat cggataaaaa
     2281 gtcgtaacga tcgtcaaatc ctcttttttg tctgcttcct tgttcgtatt tccacatgct
     2341 gcaaacagta aagcacctat cattaccgta gctgtcaaaa gatatttttt catttatatg
     2401 ctcctttcaa tgaacaaatc ggaattttac gatttattaa cgtgaactaa tgtatcaaaa
     2461 taaaaaaaca cagtcaatag aaatccatca atttctgatt taagcatgta tattcttgat
     2521 taaatcgtaa aatggatgag ttgctgctca tatctagcca tttcactaca ataaaaaact
     2581 gaagaatacc attttaggat tcctcagttt ttcatcacct attttacttc ttatatttta
     2641 ttgcagggtt cgtactaaat aaacttcctc acaaaaacct tttaacccat tatatactct
     2701 ctgcgctctt gaataatgtt gacacaaggc aaaaacggtt ggtccgcttc cactcatcaa
     2761 tgcagcatct gctccgtact tgatcatacg ctctttgatt tgatggatga ctggatgccg
     2821 ctttcccgtt atatcttcta aactgtttcc catttttttg atcatctgct gataatcttg
     2881 ctctcggatg gcttcagcaa gtccgtggat gtcttgatgt tggatggctt gcgtatctac
     2941 ttctctaaac actgtacgtg tagagacact cattcttggt ttgaccaaca caacccagca
     3001 ctgtggcata gaaggtaata cttggatttc ttcccctttg ccaccgacaa atgccgtact
     3061 tccgtagaca cagtaaggaa catctgtacc gacttccaaa ccgatttctg ccagttcttc
     3121 taacgaacag cccagattcc ataattggtt gatccctctt agagcagctg cacagtctgt
     3181 acttcctccg cctaatccag ctgctactgg aatattcttc ttaacaaaga tcttgacacc
     3241 ggttttgata ttgtatttat tttttagcaa taaagcagct tgataaacat tgttccgttc
     3301 atctaccggt aaaaatgctt tatttgtttc tacaatgatt ttgtcttctg ttatttcttc
     3361 aaaaaataga tgatctgcca agtctacgct cgacatcacc atttccaact catgataccc
     3421 atcttgccgt ctatataata catccagtcc taaatttatc ttggctggcg ctttttcaat
     3481 caattccatc ctctcaccca ttcgaaaagt ctaagtcgca agtattctat catagggaaa
     3541 aataaaagta tagcttatgt ctgcctagtg gttgattttg tcatcgtttc aacagctttc
     3601 acagaatttt ctcttcataa tccaaacaag ttctagtgat tcttataatt ctgtgagcac
     3661 ttcttctgcc aaactcggaa catttccttt ttcttttcct atcagactga gtagctgcat
     3721 ggtagcaaat ccgaaattac ttcctttcct ataagcaaaa taacgtcctt cccaatgatc
     3781 gacatatttt aatttctttt cataagcaga ctggaaggaa tagaaaaaat taccataatt
     3841 atatacgata tgcatgactt tttcttgaaa gaaagaaaaa tctgtttctc caacattagc
     3901 taatggagct aagcctagat tggcttccga gtagcccatt ttctcgtata caataaacag
     3961 attggctaac agatagtcag ctaattcctc agattcttta ggtaagaccc gcaacaaatc
     4021 atatcccgcc tgcttcttcg tcatcggctg agcggtgatg aagccaacga ctttttgatc
     4081 tttacggaca ataccgacac cgctagataa tacatatgat ttatcaaatc gtcccacaga
     4141 gaagtatttt tctcgtgcac cttgaagcca tttttgtgaa acttcattta cagcttgatg
     4201 taattcttcc ggcagttgat cgtaccaagt aaaatggtat ccttctttca tcaaacgttg
     4261 atattcaagt tgctgactca gccgagacaa gtcttctgtt gctagatcaa caatccctgc
     4321 ttcacctact ttcgtgaagt gaaaacccaa ttcatgaagc atcactacgt agctttctga
     4381 tatcttataa aacgcaagct gatagcctaa acaatctgct tgtttcataa actgattcgt
     4441 tgctgcccgc atcattttag gattgccaac tggatctcct aaaacaaagc atttgtttcc
     4501 tttgatctga taaccaaaaa cgacttgatc ttggtcgtct tcttggtaat aaaaataact
     4561 gtaacctgac aagcgaagct gatggcttgt agctgtgcct ccgtatgttt ccaataaacg
     4621 gtcaaaacgg tctttttcgt actcactgcc aagcttcgta gtatcttcag ctaaatattg
     4681 ataaagcgcc actaaagcta gcaatgagat gcccaaacca ataagccctg agaaccacac
     4741 atcatctgaa ggaaacaaaa taaaatgatt tcctgttatt cgaccgacta aaaagcgtcc
     4801 gctgtgatag cctgcaattg cgtaaacgat aaacaaaaag ccgaacaagc tagcatcaaa
     4861 gattatagct ccccaagaat aaacgaattt tgttcgataa aattcttttc tggataacca
     4921 cacagctaac agaatcaaga catataccaa tatcaactgc caagataacg tacgtgtgat
     4981 tgtatttaaa ataccaaaag atagtaaagc aatcgtaggc agaaatgcct ttttgacttt
     5041 tactgaaagt gctctagcaa ggccgagtaa taaaaagcca accaatagat tcactgtctg
     5101 atctaaaaaa ttgaatgaga acggcaataa tacttgaaat agccgactga ttgcagacag
     5161 attagtgatt gttgatagaa gtaccatcat gatccccgca aaatagacag cacttacgag
     5221 cacgatatgc gcaacctttt ggaaaaacag tcgcggaatg ttatctaaaa atcgattgat
     5281 tttgactcct gtatgtgtga tgaacaatag aagtcccgtc ataaaaggta gaacataata
     5341 aaacaaccga taatataata gccaaactac tgctacatct tgccgaacag acaattgact
     5401 cattcctaag atcatcaaaa cgtcaaaagt ccccattcca cctgggacca tcgtcagcat
     5461 tcctatcaat gtagcaatca tgaacatagg atagatcgaa agcatcgaaa tatctattct
     5521 catcaacttg ccgatgatca aaaataccgc aagggctccc gtccattgtc caaaggatgc
     5581 cagtagcaga ccaagcaagc cttttggata aaactcgaag aacaatgtct ttctctttag
     5641 ataagcaaaa aagaaaatga ctggtgctaa aaaactaccg ccgaaaagcc agatccaata
     5701 gcttcgaaaa atactttctg actgaataaa aaagacatca ataaatgtaa gaaacgacca
     5761 tacagacaaa ccagtcagca taaatagtgc gacttttgat acagtggcta aaaccatttt
     5821 tctatttgtc tgctttccat aaaaactagc ccgcagactt gctcctacca caccaccaaa
     5881 accggcgaga ttgttgatcg tgtttgttac ccacgcagca agaagaaact cttttctttc
     5941 catttttggt tttccttgtt tttctaagac tctgatcgtc acccaatcat acaacaacat
     6001 cggagtgaca cccagcagtc cagccagaat catgagcagg atcgtcatgc ggctttgttg
     6061 tcccatcctt tgccaaatat cttgccaact catgccatta gcaatatttg cgacttgatt
     6121 tgccacaaag actaacacgg agccgaaaaa aatcagtttt aaaatcagac tatgttcctt
     6181 tatccattgc agcatcaagt atcgtttatt tttcacattt tcccattcct aacttgtata
     6241 cttttgtgtc ataccccaat gttatcttat ttattttaat ttttccactt tagacctgtt
     6301 ctttgcttgc gatgatagaa aaagaaagat atcctaatgc taaaggagag gataacatga
     6361 aagtaggatt tatcggcaca gggaatatgg cacaagctat tattgatggt ttattaaaaa
     6421 aaggaacagt ctcgaaagaa gaggtgttcg tttccagtgg acattttgaa aatgcacaaa
     6481 aatttgctgc tgaaactggc gtccatgcat gcaaaagtaa tcaggaagtt gcatcacaat
     6541 cagatatcgt tcttttagcc gtgaaaccaa aaatcattga aaaagtattg gaagaactaa
     6601 gagaatatag ccagaaagtc ttattcattt ctattgcttc aggaaaatca ttaatagatt
     6661 tagaaacagc gcttgaagat cctcaggcct ccatcgtacg tgtgatgccg aatgtcaatg
     6721 tcagcgtagg agcaggcgtt tctgcgattt gcaaaaatga agctgccacg gaggaacagc
     6781 ttcagcaagc tacgatgatt tttgaagcaa tcggcagcgt ctatccttta gcagaatcag
     6841 atttcagtac attcattggc cttgctggca gctcacccgc attcgtttac atgctgatcg
     6901 atgcgatggg acggactggt gtactgaatg gtttgtccaa acagacagcc actgagattg
     6961 ctgcaaaagc aattatgggt tcttgtcaaa aattcctttc aagtgataaa aatccatggg
     7021 aacttgtaga ccaagtttct tctcctggcg gtacaactgt tgcaggtgta gttgccttag
     7081 aagaagcagg attcatccca gcaatcatca aaggaatcaa tacgacgatc gagaaagatc
     7141 ttgagatgtt gaataaaaaa taatgatagc aatgtttttt cgtgccttac aaaaaaagaa
     7201 gagctgtggc ttttgccgcg gctcttcttt tgtatttttt attatttagc ataatcagtt
     7261 gcacgagttt cccgaataac gattactttg atgtgtccag gataatccag ttcttcttcg
     7321 attcgtttac gaatatcacg tactaaacga acagcgtcta aatccgagat ttcttctggc
     7381 ttgaccatta cccgaacttc gcgtccagct tgaactgcgt aacttgattc tacaccggcg
     7441 aagctgttcg aaatattttc cagacttctt aaacgacgga tgtagttttc taatgattca
     7501 cttcgcgcac caggtcttgc tgctgagagc gcatctgctg cagctacaag gacagagata
     7561 acagaagtag cttccacatc tccatgatga gaagcgatcg cgttgattac tactgggttt
     7621 tctttgtact tggcagctaa ttccgcaccg atctcaacgt gagatccttc aatctcatgg
     7681 tcaagtgctt tcccaatatc atggagcaac ccggcacgtt ttgctaattg gatatcttcg
     7741 ccaagctctg ctgctaagat accagctaat tttgcaacct cgaccgaatg cttcaagacg
     7801 ttttgtccat aactagttct gaatcgcaga cgtccgatga tcttgattag atctggatga
     7861 agtgtatgag caccgacttc aaacgctgcc tcttccccgt attcgcgaat acgttcatcc
     7921 atttctttac gagatttctc gaccatttct tcgatacgcg ctggatggat tcgtccatct
     7981 tgtatcagtt tttccaaggt catccgtgcg atttctcttc ggattggatc gaatccggaa
     8041 agaacaactg cttccggtgt atcatcgatg attaaatcga taccagtcag cgtctcaagc
     8101 gttcgaatat tacgaccttc tcgtccaatg atacgacctt tcatttcatc attcggaagg
     8161 gtaacgactg aaacagtcgt ttctgaaact tgatctgctg cgcagcgttg aatcgctaat
     8221 gataataagt ttttcgcttt gcgatctgct tcttctttgg cacgctgttc agattctttt
     8281 accattagag ttaactcatg attcagctct tcttcagtcg atttcatgat gacatctcgt
     8341 gcgtcctctt ttgataaacc agcaattttt tctagttctt tttgttgatc ttcaatcatt
     8401 ttttcaacgt ctttttctcg ctcttcaatt aattgctgtt ttgaactaag tgcttcttct
     8461 ttactttcaa gtgagttctc gcgtttttct aacgagtcat ctttacgatt cagcgtttgt
     8521 tcgcgttgaa tcaaacgatt ctcttgagat tttaattcta acttactttc tctcagctca
     8581 ctttcaactt cagaacggta tttctgattt tcttccttag cttctaacaa tgcttctttt
     8641 tttagagttt ctgcttcttt ttttgcacta tcgatgattc cagcagctga tgattgtgca
     8701 ccagctattt ctttctcgtg acgtgacttt gcgaccataa accctaaacc aagaccgaca
     8761 attaaaccga tgatagcgag gagaatgttg aataccataa aatccacctc catactatct
     8821 ttttcatttt tatcatctat acagcttcta tgataaatta ttctaaactc gtcaatatgc
     8881 atcttcgcat atatgtgtac aaaaaacatg cacaacttta ttctaatgtt tgtaccccgt
     8941 agtgtcaact ttaaataaac aaagaaacgg ctttctactt attcattaaa cgatggtttt
     9001 taaaataaat gacactagtt tcatataggt aaacctttaa attgagaaac ttttctatta
     9061 aattattctt tgtagatgaa cagatatttt tattttatgt acccactcgt ataattaaat
     9121 aactcacgat ttctttattc aataaaaaaa cggcaggtca aggagagtac ctactcttca
     9181 gaccagccgt ttttgtttat cataattttt attcttctac taaaggcaat tcttcttgtc
     9241 ctttttcatc ttctgggaca gcgacttctt caccaatacc ataagctgct ctaactagag
     9301 cagaaacttc tgccatcatt tcaggatgat tagccatata gattttagcg ttttcccgtc
     9361 cttgaccaat ccgatcttct ttataagaat accaagcacc gcttttatca acaatgtctt
     9421 tttctactgc catgtctaga agttctcctt cttgtgagat ccctaaacca tacatgacat
     9481 ctacttcagc gattttgaaa ggaggtgcta ctttgttttt tacgactttg atctttgtac
     9541 ggttaccaac aatatctgta ccttgtttca attgttctgc acgacgtact tccaaacgga
     9601 tcgttgcgta gaatttcaat gcgcgtcctc caggagtgat ttctggattc ccaaacatca
     9661 cgcctacttt ttcacggatt tggttgatga aaatagcgat tgtttttgtt ttgttgatcg
     9721 aaccagagag tttacgcaat gcttgagaca tcaaacgtgc ttgtaacccg acatgtgagt
     9781 cacccatttc gccgtcgatt tctgctcgtg gaactaaagc agcaactgaa tcgactacta
     9841 cgatatctac agccccactt gatactaaag catcagcgat ctctagacct tgttctcctg
     9901 tgtcaggctg tgaaagaagt agttcatcga tattcacacc taatttttgt gcatattgcg
     9961 gatctaacgc atgctcagca tcaatgaaag cggccgttcc gccatttttt tgtacttctg
    10021 caatagcgtg tagtgcaact gttgttttac ctgaactttc aggtccatat acttcgatga
    10081 tacgtccacg ggggtaaccg ccgacaccta aagcgacatc taacgcaaga gagccacttg
    10141 gaattgtaga gatttgttgg tcgatttttt cgcccaattt cataatagag cctttaccat
    10201 agctcttttc aatttttttc agtgcagcat ctaaggctgc tttacgatca tctgccaata
    10261 cgataatcct ccttatattt acaaatcaga gcaacatttc attatcctta tcatatcttt
    10321 tttagtgaaa aaaagcaaga aaaaaccgaa caaatattcg catttatcat tttcttaaaa
    10381 gctgttttct caaaagatca aatccttgca taaccgcatt atatctgata gcagcacgat
    10441 ctctggcaaa gtgatatttt tccgaaacca cgccgtcttt agatgaaata gcgatccata
    10501 ctgttcctgc agattgacct tctaacgtgt ccggtccagc cactccggtg aaagctacac
    10561 tgtaatctgt tcttgctatc tcacgtgctt gaatggccat ctgctccaca cattctttgc
    10621 tgactgttcc atattctgct aatagctccg gatcaatctc cagaaaacca gcttttgttt
    10681 ggagcgagta ggtaacgaat ccgcctggaa atacttcaga tacaccagca atgtcgccta
    10741 atgcggcttg gaaggcgccg gctgtcaaac tttccgctgc tgttacagtc tgcttgtttt
    10801 ctttcagtag ttccactacg acttttgcta gcgaattatc atcgccgtat ccataaaaat
    10861 attcccctac tcgttcttgg attttttctt ctaatgctaa aagagcttga ttccctgcct
    10921 gaacatcatt tgttttcact gtcagtcgta atgtcacttc atttggtttg gcatatggtg
    10981 cgatcgttgg attgatctgt gtctcaatca gatcttttag ctcagtgacc agtcgcgatt
    11041 caccaatccc ataaaagcgc aaaactttag aaattagttt ttcttcagaa ggaaaacgtt
    11101 cctctagtaa aggacgtact tgttccacaa acattggttt caactcatta ggcggaccgg
    11161 gaagaaggat atacgcatgt gtatctgttt gataaaaagt ccctaaagca agccctgtac
    11221 gatttggtag aggaaccccg ccttcgataa tctgggactg ttgcagattg tttttggtca
    11281 tttttcgatg ggttgtttca aaatatgcca ataatttttt gtatccttcc gtattttgta
    11341 tgagagactt accgaggtga gccgcagttg cttcttttgt caggtcatct tctgtcggtc
    11401 ctaatccgcc acataagagt atgagttcac ttcttgtttc agctagagca atcaactctt
    11461 ctaaacgctg ctgattatct cctacaactg tttgataata aacttcgata cctagatcag
    11521 ctagctgttc tgacaagaaa gtggcattcg tattcacgac ttgtcctagt aaaagttctg
    11581 ttccgacagc tattatttct gctttcatta cttcccgctc ccttatatag tatacgctgt
    11641 atttttatat ttttttattt aaaaagaggg caaggagctg tgacaaaaat cttgtcacag
    11701 ctccttgttc caaataaaca gtgttctcct gccaaaacct cggtcataag tcacaatata
    11761 cataaaacat gaaaagctgt tttttgaaat tatcccttgt gactcagagt tagacggcag
    11821 gctcacaacc tctccactaa acgatactta cattgatcct ttgaacacac cgctgttttt
    11881 gatgaagtaa tccacacctg aatagatagt aaagatcaga caagcataaa gcatgatttg
    11941 atctactgga ataccgatca ggctgaatgg gatattattg ataaacagca agataatagc
    12001 aaccatttgt gtagccgtct tgacttttcc cggccaagct gcagccatga cttctccatg
    12061 ttcgacaagt agtaaacgca agcctgtgac ggctaattca cgacagacaa tgatcgctac
    12121 tacccaacca ggggcctttc cttggtcaac caacatgata aaagcagtca taactagcat
    12181 tttatctgcc aacgggtccg caaacttgcc aaaattagtg acaaggcctc gcgcacgggc
    12241 aatttttcca tctaaccagt ctgtgatact agctaaagca aaaataactg cacctactag
    12301 ctgtgtgaca gccagtgtgg tgtctcctac gttaaattgt ccccaatcta aaggtaaacc
    12361 tacaaccaaa atgaaaatcg ggatcataca aattctaagt acagttaatt tatttggtaa
    12421 attcacacta tcgactcctt tcttccctat tctacgtgac tatcagcgaa gtttccaccg
    12481 atattattta cttttccaaa tgaatttatt caaaggagta tttttattga aattgatctt
    12541 tttgaattaa cgaagaagga ccgcgacaca gatttgtcac gatccttctt ttttatacct
    12601 acaagtaaac agatgcgtaa actaatttat acgatcattc tgcgtattga agagtaaggt
    12661 tcacattttt tggactctga ttgttttccc ctggattaac tggtacggat tctccattcg
    12721 ctttgatcgt tgcattgctt gctgcaccaa tcgtgatggt cgcatttgtt gctgtttccg
    12781 gtaacacggt actctgtgtt tcatttgctg ccagtgtccc ttgataaaca taggcattgt
    12841 ctactagcac acctaaccat acacgattga cagcagtaaa ttcaagggtt acaggcttgg
    12901 tagcgtctgt gatatttact gcgatggcac tgccagaatc ttctcctgta gctattgcca
    12961 ttttcttttc ttcacttgat tttgttgttg attctactgt agaagactct ttggtctgcg
    13021 tgctttgctc tgaggaagag gactcctcga ctgatccttc tactttaagc gaagaagctg
    13081 tgtctccgat tattggatca gcttgacgat cttgccaagt catatagccc acgacaacga
    13141 caatcgccaa agcaaccaat cctaataaaa tgacaggaag acttgtccaa aagcgacttg
    13201 gatgcgattc ttcctgatgc attgcttttc tagaagtact tactgtttca ggctgcggac
    13261 gttcagggac cgatcctgtc aatttgcttt tcccatcaaa aacatcgact aaatggtctc
    13321 catcttctcc tacagcttca gcgtattgcc gaataaatgc ccgcacgtaa aatttaccag
    13381 gcaattggtc aaaatcatca ttgtcgattg cttccaaata acgtttttga atttttgtaa
    13441 tctgctgtaa ttcatctaaa gatatttttt tattcaggcg tgcttgccgt aatttttctc
    13501 cgatggtttc aaaagccact cgttcatctt ctccagtctg tttgattctc cgaagcatct
    13561 gctgttgcaa cgaaagacgt tatgtttggg tgctgtgaca aaagtcatgt cacagctcct
    13621 tttttccgaa taaacattgt tctcctgcca actcctcggt cacaagtcac aatatccaat
    13681 aaatataaga agctgttttt tgaacttgtt ccttgtgact cggagttaaa cggcaggctc
    13741 acgacctctt tcttaaacgg tgttcaatca gctgttcctc ggtattagct caaaaaccgg
    13801 caaaatataa ggagatattt ttccgtatgt ttctctaata ctcaggtcaa aacgctgatt
    13861 tcacaacctc ttccttaaac ggtgttcaaa agcttgcatc tgcggtaata agtccaacca
    13921 agttaaaaac attaagaagc tgtttttact tggctgttct tattacttga tgcagggcag
    13981 ctttttcaca acctcttggt acacggtgtt ccagaagtag cttcttcggc aataagccga
    14041 aatatcacaa aaatttgaga agcaa
//
LOCUS       DAHHZJ010000062        13756 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.063, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000062 DAHHZJ010000000
VERSION     DAHHZJ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.063"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(<1..274)
                     /locus_tag="NBL02_001485"
     CDS             complement(<1..274)
                     /locus_tag="NBL02_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCD2572174.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRF"
     gene            complement(353..1264)
                     /locus_tag="NBL02_001486"
     CDS             complement(353..1264)
                     /locus_tag="NBL02_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCD2572175.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(1283..1627)
                     /locus_tag="NBL02_001487"
     CDS             complement(1283..1627)
                     /locus_tag="NBL02_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572176.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(1647..2203)
                     /locus_tag="NBL02_001488"
                     /pseudo
     CDS             complement(1647..2203)
                     /locus_tag="NBL02_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2284..2784)
                     /locus_tag="NBL02_001489"
     CDS             complement(2284..2784)
                     /locus_tag="NBL02_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572177.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(2785..3330)
                     /locus_tag="NBL02_001490"
     CDS             complement(2785..3330)
                     /locus_tag="NBL02_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572178.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(3327..4583)
                     /locus_tag="NBL02_001491"
     CDS             complement(3327..4583)
                     /locus_tag="NBL02_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCD2572179.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(4944..5255)
                     /locus_tag="NBL02_001492"
     CDS             complement(4944..5255)
                     /locus_tag="NBL02_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572180.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(5278..6588)
                     /locus_tag="NBL02_001493"
     CDS             complement(5278..6588)
                     /locus_tag="NBL02_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HCD2572181.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLV
                     RMLIDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGH
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     MLIAGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEE
                     MIICLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDME
                     ARNRVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(7202..7654)
                     /locus_tag="NBL02_001494"
     CDS             complement(7202..7654)
                     /locus_tag="NBL02_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572182.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(7656..8021)
                     /locus_tag="NBL02_001495"
     CDS             complement(7656..8021)
                     /locus_tag="NBL02_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572183.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(8120..12403)
                     /locus_tag="NBL02_001496"
     CDS             complement(8120..12403)
                     /locus_tag="NBL02_001496"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HCD2572184.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(12550..12789)
                     /locus_tag="NBL02_001497"
     CDS             complement(12550..12789)
                     /locus_tag="NBL02_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572185.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(12879..13151)
                     /locus_tag="NBL02_001498"
     CDS             complement(12879..13151)
                     /locus_tag="NBL02_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572186.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            complement(13234..13485)
                     /locus_tag="NBL02_001499"
     CDS             complement(13234..13485)
                     /locus_tag="NBL02_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572187.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
BASE COUNT         3942 a         2980 c         2051 g         4783 t
ORIGIN      
        1 caaaacgatt cataaaggat tcaatttcag gtgtgtacgt taattctgac cgactttgta
       61 cttctttctt ttgtaattgt tggatttcat ctttcaaaaa cgacacttga accttcgttt
      121 tcatggattg aaaaaatgcc aagccccctg aaaaacaaag aaataaaacc aaacaccaaa
      181 tccatttcac tgcttttttt gaatggattt tcttttgctt cggtactttc tctgtataaa
      241 ctttttcttt ccgccgtagt tgtcgttttt tcataagcta ctccttctag tatgttttta
      301 ctagtacgta cgattgacaa cgataagctt gtttttctga aaaatagtcc attcaattac
      361 cctcattttc tattcctttt ctctttcttt tatatccaac aaatccagca atacttgtca
      421 acactcctcc tagaacagaa agtgcaacat gtatttcttc accagtcttt ggtaagactt
      481 ttgtttttcc atctgctgtt ttgatttcaa tattgccact tggaagtttc tttacaggaa
      541 tcgacaaatc ctttaccaaa ttcccttcac tatcttggat taatggaata ccatctttta
      601 caccaacaat tgtttgatca cctgctactt ttaatggggt ggttggtgtt ggaatatggg
      661 tcaattcctc tacattacta gtgacaatcg gtacactcat tccttgagaa gtattcgtcg
      721 taatttcacc gcttggggtt accgatactg tgacttcttt tgctggaaca ataggtttat
      781 tttcttttgg aattgcttct gatttttcaa caggttgttt cggttcttct ttttgttctt
      841 cactggtaga cgacatcgtt ggctgttccg tacttgacga atcagtattc ttttcagaag
      901 aactggttgt tcctgattgt tccggagttg tgactatact tgaatccgtt gttgttgaac
      961 tatgcacacc ttcttcttcg atcgaatcag tcggtaaagt tgattcagga aactctggtt
     1021 ctggtgtcac tggctctttt tcaggtttct ctgactgcaa ttgttcaggt tctgctggtt
     1081 tttccgttgt tcctccattt agctctgtct caacatctgg taatccttcc cctggcaatg
     1141 tttctacaat ttctgactta ggtgtttcta cagtttcatc tgcataaacc aatgaactcc
     1201 ctaattgaaa cgaaaataat gaaattccta gtgttgttac gactgctaat gttgtttttt
     1261 tcatgattat tcctccaatt ttttatgcaa ttcgtgtttt gttcatagac gccgcactaa
     1321 tattatctga acgataaaag aactcccgtc cgttttcttc taacccaaca tatggattat
     1381 ttttgaataa ttgaatcaat agttcactgg tgttgtattt tggatagttt gcaaaaaaat
     1441 aatactgtgc ttgtcgccca tccgaaaatt caacaagtaa ttcataacga taccgcttca
     1501 atccttttcg ttgttttaaa ggatctctat accaacttgt ccatcgttcc attcgtggat
     1561 agagatagac acgatacaca tagaacaatg ccataccaca taccaccaaa aataataatc
     1621 gccacataaa aattccccct tttcttctac ctttcttgaa tcatcgaaca ttcataccct
     1681 ctatagagat ttgaaaaatc catcatcaat tgttcatata aacgttgttg caaatagcga
     1741 ttgtttcgaa tacataattc ttcatagcca tcaatcactt gatcttcttt ttcatagcat
     1801 cttgtagctc tatgacgctc agcttgcatg acataaccaa actctgcacc atatcgttct
     1861 actgttaata actgttgact acgcaataat gcaatataac aatcacaaaa atgttcaacc
     1921 gacatttgat ttttctcacg tttgtgaaat aggtattctt tagaaagcac attattcgtt
     1981 aaacaatctg ataaatagcg ataatctggc ggtcggttat tttcatctgt gaatttttcc
     2041 cataataaac tgatttttat tcgttcagcg gctagatagt cttgttcgcg tggataaaat
     2101 tcatcaatcg cacaaagttt tttatcacct gaataaacaa atccatataa tgcgaaacga
     2161 actcttcctt ttcaattggc tctcctaaaa attccagtga cataccatct tctccttcct
     2221 aaaaaaaata aaaaagcaga aaccgatttg gcttctactt ttctttcctt ttatagctct
     2281 tttttacgga agaatccgca aatgacattt tttcaaaaaa tcaatcggca caagttcacg
     2341 aaaacttgga gcaattttgg atagaatcac ttccccatct tgtaaaatac aatcataaaa
     2401 atcatcagtt acttgatact tacgcttctt acaataattg ataaaatagt agcgatcatt
     2461 ttctatttcc attcgttgtt ctactaagcg catacgaaca tcatcataga aataagccac
     2521 aaatcgccct tcaagttcat gtttttcaat gggatactct tctggtaaca atctattttt
     2581 tcgtcgttta tacattgctg gataccttag aagaagttca tctaaacgtc tgtcagtata
     2641 tggtaactga cgaatcaacg attctaatcg ttctttatac agatagaata attggcgcaa
     2701 gcaatagtgt accgcctctg gattttctaa tactgttgga tcacgtgacc aatacgcacc
     2761 aacataaatc ataatttttt tcatttagac aatcacacca gcaatactaa tgaccgatcc
     2821 acaataggta gaacaagtgg catagcgacc aatttcctct aattctatct caatctcttc
     2881 taaagaaaga tatgtctgtt cttctgttgg ctggcgactc atttcccaag tgacagtcac
     2941 caatgaaatt tcttccataa gaggtttttc gctgctaagt gctgccacac agttttcata
     3001 tatcttcatt ttatcgcagc cttttttaac cgctaaaatc gcaggtattg gcaatgaaaa
     3061 tcgataaaag cgaatgaatt ctagccttcc tttccttcgg ctaaattcca actcttcgag
     3121 ttcttgttcg tatcccttac gaacttctaa taagcgtgca atttcttgcc agttctcttt
     3181 ttcctgataa cgtccagctt ttaccgcttg ttcaatataa ggaactcgaa aacgaaaata
     3241 agacaaatca accatctttt catttaatgc ttcgattgca gcctttaaat ccaacaggcg
     3301 cagccattct gttgtcgtat atgcattcat gcaattttcc ctcctgacgt tcccatccat
     3361 tctagatagt tttgttgctc gtcatttagt tcggcattat caatcccttc ttgtacaaag
     3421 tctcttccta aaatacgatc tgcttcttgt aacaacttta atgtcggagc tacttgtttc
     3481 aaaaaccagc gttctgaacg atctaatgag aatttttctg gctttactgt aaaatccaat
     3541 tcttccatcg aacgaaataa ttttgcccat cgtttatcaa caatccgtgc atcactttcc
     3601 agtctatccc aaacaataag ttttgcatta attaaaccaa cgccaatctc tcctaaacgt
     3661 ttcggtgaat cacctaaaat aaatgcttct actgcttgtg ttgctttctc attggctaac
     3721 cgtaactcat atcgattata aataccatat ttcgttaatg cttctaattc tgaaattttt
     3781 tctcggttcg caatttcaaa acgtttctca taaaaattaa aaaagagagg actctttcgc
     3841 gatccaaaat acaaagataa tccttcactt cgaaaatagc catcatctaa aaaacaaccg
     3901 ccaatattct ggaatctctt aaagtccatt tctaataaac ctgctttttg tttttctagt
     3961 aattcaaaga gatcgaaatg ttcttccgtt tccaatcccc aaagttcatc taacgcaata
     4021 tcgaatcggc tacattgcac acgtgtataa ccagcttcca actttccttg atttttccaa
     4081 atggaatata gccatgttcg ccaatctgtt tgttgttcac gcatgacttc ctcatattcc
     4141 cggcaacctt gtcctgaaaa ttgcacaagt acattttctg ttacagttcg ttttgaatgc
     4201 cagaacaccc agatattccc acgaacagaa agacagtcaa aaagatacaa gcccgttgga
     4261 cgtgtttcaa attctgtcag atccattcgt aatacacgtt gaatcactgt tttgtagtct
     4321 gtcccaaaaa aggaaagacg caaataatca atcattgctc gaagatctat tccgcttttc
     4381 atatacaatc gaaagcgttc cttgattcgt tgttcaaaat cctctgggat ttttcttttc
     4441 ccttgttcca tctttctaat gtaatctttt gagcagccca tcaattgcgc aaatcttgct
     4501 tgtgatagcg gctttttatc tccacttata ttatgaattg cacttaactt ctttctgtag
     4561 ctttttagct caatcggtgt cattttctcg ctccttttat aaaataacaa aatcgatttt
     4621 ttttaaccct ttagaaacac cgatatatca actagtcccc ctcgtttttt gggaaaagta
     4681 gtacaactgc tgaccttaga taatcacttg atacaaaagg gtttataaaa cttttttcta
     4741 tgtgttatgt tatttctgcc ccccatatta tatatcgggg ggctttacac aaagaggccg
     4801 tgcgcaagcg cacgacctct ttgtgagctg cacacgctgc tagcgagtgc gcattatagg
     4861 cccatgcttc gcagccttaa tgcgcaccgc caggcagagt gcggctacca gactacgtct
     4921 agagccctcc cgatagataa taactacaaa attaaatgat ctaattttgt tatttcacta
     4981 tatttttcag gagaaaaaac gcgataaatt gacaaagtta aatcctcttc taaatctact
     5041 cgataatgat acaatcgagt caatcgttct atatcttttt tcgttagcaa tacttgatta
     5101 ataacttttc ctgtaaaaac acgttctttg cgcaaaaagc ggtaatcagt tacttgttca
     5161 atacgattca accaatcata aaacgttctg gaagaaagag aaggattaat cattcgtatt
     5221 gctgtagcta ctctataata acaactctct gccatttaac ttcacctcct tagttgatta
     5281 caaaatatgc gcatcagaaa aacttgttcc ttttggatct tttgctaaat acatttggcg
     5341 ctctagttgt tcttgtaaca actgattatc atctcccatc gtttgatctt gactatcctc
     5401 ttttactgta aaatcataga caatcatttc agtaaatttc tcaaattctt tgataaagtg
     5461 ataatctttg taaacaaatg gcgcaaaaaa ctctacaggt acatcaaatc ctggaaaagc
     5521 atatccacga ccaggttgat ctttgttgta aaatggctta tttttaaaat cttctccaaa
     5581 agtcattaaa taaccaatgg gagacaatcg accaacagaa atacggaatg ttaaattatc
     5641 ccgaacacca cttggaaaat attctgcatc tggtcgttgc attgctaaaa taatgtacat
     5701 ccctgcttga cgacccttta atactaatgg ctgaatcaat ttatcgactc tatttctcgc
     5761 ttccatatcc aacgaaccat aaaaagctgc ccattcatca aaaaagataa aatgcggtgg
     5821 taacccataa taagcataat ttttccctgg ttcatattta ggatctttca tggttttcat
     5881 ataggcatac cgttgattca tttcttttaa tgcatttcgt aaacaaataa tcatttcttc
     5941 atttattttg taaaacacat gtcctttgaa tacatcgacg tcacctaatg ctgctaaatc
     6001 tgccgctttt ccatcgcaaa tatctactgt cccaacactt aaacaagctt gaattaaaga
     6061 atacaataaa taggttttcc caccaccgat tgcaccagca atcaacatat ttggtgattg
     6121 atcatatacc caatccaaag ttttcattaa atgaatacgt ccattttctg ctatacattg
     6181 atcaatagga atccgaccag aatctgaaag gaatacataa ttgattcggg ctttttctcg
     6241 ttcttcacga attaaatcac gcaaaaatac ttcagataac aaatgaccca tttttaaaaa
     6301 tcgttcgtga aaacgcccac catctaaagg aaaagaaacc actaattctt gtttcttttg
     6361 ataaaaataa acttttggta atctcatttt ttcccttgtt tgtttctcac taacttgttt
     6421 ctttgtataa acaaaccgat tgtcaattaa cattcgaaca agtaactgac gccaataaac
     6481 agaggcaaaa tatccttctt ttagtcgcga ccaatgcatc aaccctaaaa ttaacccact
     6541 aaccagcaac aaatacaaca caatacttat tccaggaata agatacattt gccaattcga
     6601 aaacgtactt aaacgtggat aaaatacttt ccaactaaac caacctaaca gaatcaaaaa
     6661 tggaaacaaa aagaaaccat aatagcaaaa ccataaataa cgattgaaat agcgaattcg
     6721 ccgaccacga tacttgaaaa aatgagcttt caattgtcac attcctcctt tctgttttta
     6781 cttttcctgt gctaaaaact tttgacaaaa agcaactaat acaggaatat cacatttagt
     6841 aaaaaaacat aatcgaaaaa gatttaatcg atttttataa gggtgtgcac acatattaca
     6901 tattcgtact atacgaatcc aaatcacttc ctgatgatct tgtttccact ggtacggtaa
     6961 tgaaaaacga acaagatcac gtaaaaacat acactgccca ataaccgcac aaatatatgc
     7021 atttgtttgt acaaaaatcg ttggattgtc tccaaaatac tcaatcaatt gttggatatt
     7081 ttgataacaa gcacaatctc tcaaagtatt tgattggttt tatctgaatt caaaatctcc
     7141 ccttccttct atcttagata aaaagaatag gaagactcct tcctattctt tcaaaaaaac
     7201 cttatttttt ctctttttcc ttagaatcaa cacttggttg tttttgatct aaaccttttt
     7261 cttgtgattg tttcatatta ggattatttg ttggagcttc tactttctct aatcctttcg
     7321 cataaatcac taaatctgaa tattcacgat tgccatcttg tgctggaata tataccacat
     7381 gatattctgg atcaattaat ttcacaggat cacgtcgttt aaaaccagtc aaatctacat
     7441 ctgctggaaa tttaactcgt cgtgcaatct ttggttcatc tggatttcca aaaactaatt
     7501 gtgctcgctg tgatcgaatt tctcccgtat aatcgcctgt ttcattatca aatacacgat
     7561 cagactcttc taatgcgcca aagcgtacgg ctcctaaaca agtttcatct aatgcttctg
     7621 taatgtcata attatttgga aattttaatc ccatcttaca aaacctcccc aatttttaca
     7681 ccagttaaaa agacaccaac atcttgatag ccagttgttc gacgctctgt ttgtcccata
     7741 tcatttgttt cacgactatt cactcgttct ttacgaaaaa caaaataagc ttcttcaaat
     7801 gaaatacgca cctctttttc tggtaatcgt aatcttttat cctcttcagc tgtgagataa
     7861 acaaaaaaag caggctgttc ttctcgcgaa cactctgctt gtaattgaac atatcgccca
     7921 gacacaccat taatttcctt tggtggatta ggttcccgtg aacccaataa aaatacttga
     7981 cctaaaaact ctttcttcat acgcaatgct ttttctgcca taaagttttt cctcctctta
     8041 aaaaccaaaa taaattctaa atatttctac attacaatca aacaagcggt taaaaatcct
     8101 aaccgcttgt tcctcatgac taatctttgg ctttacgtac tttataaagt gttataccta
     8161 aagcaatgac taatactact aatccaagaa ccataagtat actacttgtt tgttcacctg
     8221 tttttggcaa cattggtaca acagtagctg gtttaacccg ttgttctggt ttcactggtt
     8281 gcggtggttt tggcgcttcc acaatttgaa ccgtttgatc tttgtttgtt agatctacat
     8341 tgtgtttcgc cacttcttca aacgtttctt tctcatcttc cgttttcgct tggagccatt
     8401 ccgcaaaact caagcctttt cctgcaagat tgcgaccgtc cactaagaat tcaaataaga
     8461 attccatcat ttctttatcg gccgcaaaga ctttttccgc atgagtgcct tcaaatggtg
     8521 tgccgtctgg tgttaagtag ccttgaacaa ttaagcgata gatttgacca atcgcaagac
     8581 cgtctacttt gacccattca agcacagttt gatttggcaa ggccaagatc tcttttccgc
     8641 catttttatt cgtcgctttc gtcgtaatgg ttggattact gattttgatg gtttggtcat
     8701 tgtcggtaat atctgcgtga gaagccaata atacgccttc tctaaacaga tcttcaaaga
     8761 ccacgacttc tttgcctcgc aacgcacgtg cgtctagaat aaagtctaga ttgactgtac
     8821 catccgttgt ttctgccaca aacgttaagg tacttgtgac ttcttttcca tctactagca
     8881 ctggtttttc cgttactttg tccattagaa caccgttgac tgtgtatgtt tttccaacga
     8941 ccaaatcgtg gtatttcaca gcgtctgtaa tggtgacttt cgttaatgga ttaacctctt
     9001 tcaagccagt gggtttatgt gtagccgttg tacgtacact tgggttagtt actcgtaccg
     9061 tttgcccttt gtcgttgatg tctttatggt ttgctagttc ttcatcattt ctaaaaagac
     9121 tttcaaacac gacgatttca cgaccattta tacctgtcaa atcaaaggta aatggtacga
     9181 tcaccgtttc attttctgct ttggcaataa agccaacact cgtttctacc gtatttccat
     9241 gcacaagcaa gggttcaccg gttgctttat ccattaagat ccctttgacc aagtatgctt
     9301 ttcctggaag aatctcggta atttcaacgg tatcgtttaa ggtaaacaat cctgttgggt
     9361 ctgcgactgt caatccgtct tcaaagacag ccgtggtatg aacggttgga ttattcactt
     9421 tcacactttg ggcttcatcg ttgatatccg catgtgctgc aacttcaatg ccatcacgga
     9481 aaagtttttc aaaaacaacg atcgtttgac ctttcagaac actagcatta accacaaatt
     9541 caaggtcttt tgtgccattg actttgtctg caataaaggc caaggtactt gtgatttctt
     9601 tgccatcttg taaaattggc gcattcgttg ccttgttcat caatactcct gaaatggtgt
     9661 attcttttcc gacaaacaag ttggtataag aaacactgtc tttaatgacg atcgtttcta
     9721 atggatcaac cactttttca ccatcggctt tatttgacgc ttgtgtttct agttttggtg
     9781 gaatcaagta attacgtaca gaaaccacta acgtttcccc atcatctggc gcattttgtt
     9841 tgaatgcaac cgtgaatgct tgtttatctg tatttggttg gaaactttct aatggggcat
     9901 tttccaagaa gtaatagtca tcggcaacta gacctttcac taaaatttcc ccgttttgat
     9961 cagtggtata ttcaccaatt gcttgatttt taccgttata caaggtaaag ttcacacctg
    10021 cgagcggcgt tttcttcttg ttccattctt catacttgaa taattttaca cttttccgtg
    10081 cttgacggtt cgcaaaatgg atcactttga tcggttctgt atcattggta atcgagaaca
    10141 ctaatgggtt ttgattcaaa acgtaattta cgggggcttt gatttcttcc aaacgatagc
    10201 gatcttttcc ataagtaagt gtaccgttag ttaggaaaaa gcctttttca ttggttttaa
    10261 agatatccgt tgtttctgta tcatttggaa tctccattgt gacataacga ttggctaaag
    10321 tatcaaaaat cttgaattct gtattagcaa gtggaatcac ttttcctgtt tcttgatcca
    10381 ctttcaccac tttaagacgt tgttcaatta ctttatcttc taaaacataa tggaaagttt
    10441 gcccattttc agaaattgtg acttcaagtg gttcaaatag tttataccct tcttttcctt
    10501 tggtttcaga aagaatgtac gtatcaaaga caacggtatt tttaccaaaa gtagcataac
    10561 catcattgtc tgtcacaacg gttttcacca ctttgccagt tgtttttgac gtcaaagtga
    10621 tttcaatgcc agctaatttt ggtttgttat ctggattcgt tgaatcgact aaatctttat
    10681 tagcaacttt cactaaatca atggttcctt tgatcacttg atcttttgcg gtcgcattga
    10741 cagtttttcc aggttcaaca acaatttcaa ttgcttcatc agaaagcaca taaccttttg
    10801 gcgctttgat ttcttttaaa acatatttgc caggatcaag atctttaatt tctgcctgta
    10861 ctttgccgtt aatttttttc aaggtaattt ctttaatctt tttcccatct gcttgataca
    10921 aaccatagac cgcattttct aaagaagctg caccttgtgc tttcacacca gtttccgcat
    10981 cttctttaaa cacacgaatg tttccttgtt ttagaatttg taaattaata acagacgcat
    11041 taggttctgt tggatgtaac acaccaattg tttgagctcc tggacgacga taggctaatg
    11101 gcgcaccttg aactttaatt ttattcaatt gaatttttgc tttatccggt gcctcttttg
    11161 tgactgtaat ccatacttca ttgccgtgaa tagatacatt gacacctttt ggtttttgaa
    11221 tgtctttcac atatttcaaa aaatcatttt tattctttac tttcaacgtt tcgccaattt
    11281 ttaccgtatg tgtttgatta ttaaaagttg ctttttcacc aaagttatta atcaaacgtt
    11341 gaatttctgc ttttcgacga tcataatcaa cacggatatt tttgattggg atttttgtgc
    11401 ccaaagcttc ccaaatcata aattgtgccg caatgtattg ttcatcggat ttgtctttat
    11461 ttgtgacata tcctaaataa gagatcaagc ttgttttttt ccgtacattt gaatttttca
    11521 aatagttttt caaagcagta gctgtataaa cttgaccttc tgcggctgag acaccaggct
    11581 caatacaaaa cgccacttgt ccattaatct caaaacgaac catataattt aaagtgaagt
    11641 ttagattgtt cggactagaa gtaacaccaa tattttcgcc ccaatcaatt ttccgaccac
    11701 ctaaaacagc tgcattggtt gcacggcgaa cacgaaccga acgattggtt ggtactgtta
    11761 ctaacagtgt aggtgtacct gtttttggaa tgttctgctc ttttttcttt tgattttcaa
    11821 tactagccgt agattctttg tttggtttcg ttggttgatt ttgactagct gcggttgaag
    11881 agattgtttt ttcaacgttt tcctcatttt ttccttcttt gtcagcagta gccgctggtt
    11941 gttccactgt ttcttctatt cgtttctttt cttccgcttg tttctcagaa gactctggct
    12001 gcacagaagg cgttgcttcg gcagcttttt ctccttctag agtggacgtt tctgaagcag
    12061 gggttgtaat tgaggcagtt tcttcaactg gttgtgaagt accaggacta ttccctgggt
    12121 gttgtgtttc cccaatcgtc tgatcgagag tcgttttgga attgaccgtt ggcgcttctg
    12181 tcacaggggc ctgttcaaca gtggcttcac cagctgggac cccggcttct gctggttgga
    12241 cttctgtcac ttcagaagcg acttgggcaa tggcttccat cgtaggttgt atagccgtcg
    12301 tttcttctgc atgtgcttga gcagttccca ccaagaaaca gaattgagaa aaagccacca
    12361 atgcgagtgc tttcaaccaa ttcattttcg tttttgcata cacaagaatt cctccttttc
    12421 ctgagaattt tatataataa aacaaaacgc cgttaccaaa agtgataacg acgtgaactg
    12481 tctcttctat ttttatctgg cgctcttatc tgaacacatt tcatcaatga ttttaccctc
    12541 ccactataat tacagaacaa aaaaagaacc tgcttcgtgg aaatcaactg tccatttccc
    12601 aattggaata ccagcttttt tggcaacttc accatcacca aatctcatgc cataccaatt
    12661 actaccttca tccttaattt ctttccaacc atatgacata gcttcatcta atgaatcaaa
    12721 aatcatgaca ctattgccaa ttgttacagg atctggctta agatcttctg gaagagaaat
    12781 atcaatcata ccataatgat ccgattcctc agtattattg tcattactag ctactggttt
    12841 ggttgttcta ctggcttatt ttgatacaac aagtactctt attcttcttt tacttctccg
    12901 ttttcaattt cccattcaaa ccactccgca aagtcttgct taaaaccatc tactcgatac
    12961 ctattttgta aataggcatc ttctgacgtc gtaggatcta aataggtagg tttattctct
    13021 ggacgagcat agacacctac ccatgtttcg taatctcctt caaaataact atctaccaaa
    13081 tagccttttt ttctaagaat ctcccgaact tgatttttaa ttattgatac ttgttcaaaa
    13141 ccatcactca ttctatcacc tcaaataact ctagattctc ataaatattc ccgacaattt
    13201 caatcctatc aatgatctct aacaaatcgc tacttatgct tccccattcg tataagaatt
    13261 tcccttccgc aaaataaatt ttccataaac ttcttcatga tcgtcccaac caatatcact
    13321 ctcgaaaaat tccacgttgt tcttatcttt tagctcagta aatgccataa gttcaacatg
    13381 ttttccaacc tcgaaacaat cacaccaccc atcaaccgtt acacgtgtgc cttcaatacc
    13441 taagaaactt tgtcctcgtg tattaaacac taaaactttc cgcatttttt gctcattaat
    13501 taatcacgcg cgaaatctag gaatcacaac ttattcgctt cttctttata aataaaaaaa
    13561 ctccaataat cggaaattac tatcttttcc tcattccttt tttaaatctt catacttctt
    13621 cttatctttg tcctaaccta ataacacaaa ttttgaagaa aaactcagta aaacctgatt
    13681 ctttttttat cttcacaaca accaactttt tcttcagatc cttttactag aacttgtata
    13741 caaaaattgt tgaatg
//
LOCUS       DAHHZJ010000063        13574 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.064, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000063 DAHHZJ010000000
VERSION     DAHHZJ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.064"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(<1..1489)
                     /locus_tag="NBL02_001500"
     CDS             complement(<1..1489)
                     /locus_tag="NBL02_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCD2572188.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFS"
     gene            complement(1482..2933)
                     /locus_tag="NBL02_001501"
     CDS             complement(1482..2933)
                     /locus_tag="NBL02_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCD2572189.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIRESKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(2947..3963)
                     /locus_tag="NBL02_001502"
     CDS             complement(2947..3963)
                     /locus_tag="NBL02_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCD2572190.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            4183..5271
                     /locus_tag="NBL02_001503"
     CDS             4183..5271
                     /locus_tag="NBL02_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HCD2572191.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            5325..6851
                     /locus_tag="NBL02_001504"
     CDS             5325..6851
                     /locus_tag="NBL02_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCD2572192.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            6853..8058
                     /locus_tag="NBL02_001505"
     CDS             6853..8058
                     /locus_tag="NBL02_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCD2572193.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            8315..9292
                     /gene="guaC"
                     /locus_tag="NBL02_001506"
     CDS             8315..9292
                     /gene="guaC"
                     /locus_tag="NBL02_001506"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCD2572194.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            9556..10980
                     /locus_tag="NBL02_001507"
     CDS             9556..10980
                     /locus_tag="NBL02_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCD2572195.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            11441..12550
                     /gene="pdhA"
                     /locus_tag="NBL02_001508"
     CDS             11441..12550
                     /gene="pdhA"
                     /locus_tag="NBL02_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HCD2572196.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            12553..13530
                     /locus_tag="NBL02_001509"
     CDS             12553..13530
                     /locus_tag="NBL02_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HCD2572197.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT         4334 a         2334 c         2941 g         3965 t
ORIGIN      
        1 aactgaaata gttagggtca ttataaccaa tttcaaaagc aatatctgaa atacgatagt
       61 ctgattgctg caataatttt tttgctaatt cgattctttg ttctgtcaga taatcgatga
      121 atgtttggtt aaacgattga gaaaaaatcg tactgaaatg agagggactt aatgcaactt
      181 gttgagcgac ccaagttaag gacatatctg gatcggtaaa attttctttg ataaaatcca
      241 atgcatggtt caaaattggc tgatatttgt tccttgaagg ctgtgagggc agagcagtta
      301 agaaagaaat gaacatataa actgtcgaac ggtaaagcga gtcgtcggcg ctgattgcta
      361 agcgttgact ggtggatgtg caatgaacca tttcttgcgg gattttcttt tctttcaggc
      421 agacatgctg gagtagctta gtcaagacaa ataaacgata taatcgtgtt cgagttggtg
      481 tgccttgtgg tttcgtcaag tgttcgatgt acttagatac ttcgtttttc gataatgaag
      541 acaaatcaag tgagaggtca aagtaaaaga cagaatcaac tggaagtttc gcttcttgaa
      601 tcaaatcctc atagctgata atttgttctt taagggaact ggcggtttca agtatcatct
      661 gcgtgatctc aaaagatgca ggtaattcgc tgattctttc tgcgacagga ccaatggcga
      721 cagaaaaatt atttttttgt cccttttcta attcatggac caacgtttgg gctacctgat
      781 aactggtctc gatcacattt tcttcaagag aatcaactat taaaaatttg atgaatcgag
      841 aggaaacagc agagaagatc acttgttgat tctccctaaa taaaaacttc aaataatcac
      901 tgagatgagc gtaatcttca aagtctgtac tgacttggtt tgctgctaat aatacggtca
      961 tttttttccc tgctaaagag cgattgaaag attcagcaag tttcaatgtt tcactgatcg
     1021 taagtgagcc tttgaacaaa ccgttcaaga aatgattttt tttcaattcg aaaacaagat
     1081 cagaagaagt gtgcgtttct ttttgctgca aatctaaagc agttactgct ttttctattg
     1141 cttgtttcaa ttcgctactt ttaatcggtt taagcagata atcatctgcc tgtacctgga
     1201 ttgctccttt catataatcg aaatcgtcga atccactaat aaatatgaga cgagtccaag
     1261 gaagtagttt ccttgcttct tttgaaaaag tcaacccgtc catgaaaggc atttggatat
     1321 cagtcagaat gatgtcgggc tgtaagtcga taattgaaga cagtgccatc tcaccatctc
     1381 cagcttcgcc gacataagtg attggataat aagaaccaag gacagttatt tgttcccgca
     1441 agttattgcg aatgaggtat tcgtcttcga tgataaaaac tttatacatg tttgtttcct
     1501 ccaattgttt tgggagatag agggatcatc agtgtgacag ttgttccttc tcggtatttg
     1561 ctgtcaatcg tgataggttt ttgatcattt ccgtaataaa gaaggagacg cttgtttacg
     1621 ttgtaaagtc cgtagccgat actgaaatct gaatcgatcc ctttggctaa ctctttacgt
     1681 atttctgcca gtttttctgc agggatgcca atcccgttgt ctataacttt gaattggatc
     1741 atctcatcaa ttttgcatgt ggtaatacga ataaatcctt tgtgacggac gtacttcgtt
     1801 ccatggtata cagcattttc aatcaatggc tgcaagatca tttttaaaat agaaacagga
     1861 aaaagtgttt ctggtatatc gatcttatag tcaagcatat ggccgtatct tatttttaag
     1921 atgactagat aatcattgat atggctaatt tctttttcaa gaggaatcca gtcttgtcct
     1981 ttgcttaaag acgtacgaaa aaagccggaa agtgcatggg tcatagtcag agcttcttca
     2041 aagcagtctt gttcgatcaa agaaataatg gcatccaaac tgttataaat aaaatgggga
     2101 gtaatctgag cttgcaatac acgaacttca ctttgtgcta aatggtattg tttcaaagcg
     2161 ttctcccgta aagactggtt tagatcttcc gccattttat tcatgctagc ggtaagtgtt
     2221 tgtaattcca gtgtttccgg cagggttaaa cgaaagccaa ggttccctga agaaagttgt
     2281 tctgacatct cgatcagctg attgatcggt tcttggacct ttctctctaa aaagcgacga
     2341 ttctttttcg catagaaaaa aatggtacta agaactccaa gttccactat tgttaataga
     2401 atgagcgagc ggacaagtgt cgtatttttt ttgcttgcta agtcgatctc cacacgaaca
     2461 aaatcttgta agatatctga cagcagttga gtgacagaat ctacctgatt gatgatctct
     2521 tcattggtat ccataaaatc ttttgccaag atattattga tcagctcgtt ttgataatct
     2581 tccagtgtat cgatcgtacg tagcgataca tctaacgtac taatttcttt aagtgtattt
     2641 gtattatttt tgctctctcg aatatcattc ctaagttctt ctaggatatt ttcctcattg
     2701 aactcatcta cggttacttg gccagaaatc aagtcccaca tactttccaa aactttttga
     2761 tcaaccttca aagcaattgc atctgctttt tggatatttg cgatcgtctg ttgataaatc
     2821 aaaagctgtt gcgaatataa cagtatgctc agtagcaaag ggataacagc aaatagaagc
     2881 atggctttgt ttgcttgggc aaaaatctgt tttatgctta aattttttgt catgatactt
     2941 ctccagtcaa taatttctgg ttgggatgtt agctatattc tgctgggaaa agacagtctc
     3001 ggaaataaaa atatctttag gtaatgtttt tcctgtttga atatttccaa aataacgagt
     3061 aacggtattg gctacatacc agccgacatc aggtttacac tcgactacac aattgacgat
     3121 tcctgagcgt agcttctcga tcatatcttc ttgtccatca atagttacaa tgatcaaatc
     3181 ttctccaggt ttgattgatg tttcttttat cgcagctaaa gcaccgtgag tcatttcatc
     3241 actatggctg tataatacat cgatttcgga taaatcattt gtttctaaat atcgtttcat
     3301 ctggtctttt cctttcatcc gaataaaatc accgacaatt cggtgaacaa tagtcatcgg
     3361 aggtttatgg tcgatggttc catctttttt tagggttggt ttacgattga ttgattccaa
     3421 aaatccttcg gagcgtagct tggtcggtgt ggagttctct gaaccagcaa gttctaggat
     3481 tctgatttcc tgtttctttg aatttgtgaa gtgattgctg acaaataaac ccgcccgatt
     3541 tccttctgct ttgaaactgg ggccaatgtg ggtgacgaaa agctcgggat cattcgtttg
     3601 aatatgtctg tcgacaagta tgacagggat ccctgctcgc tttgcttctt ttaatacaga
     3661 gtcccacccg gattcttgca aaggagtaaa aatgatcata tccactttat aggcaatgaa
     3721 cgtccgtata tcttggattt gccgttcctg attcatgtag ccatttcggt agagaacttg
     3781 gtaatcttct ttttcaagcg cagtgataat tgattcagta tgctttttcc gccagctact
     3841 ctccgttcct gattgcgaaa aaccaacgac tagtgggtca ggtgcttcct ttgtattagg
     3901 tgtacacccc atcatttggt aagcgattac aacaagggct aaaaaaaata aaacacgttt
     3961 cactttaaat cactccatct ggatttgtta gtactatctt atcagataaa agggaaaatc
     4021 aaagtcaaaa ggaatcaatc gaaaaaaatc caagaaactt aaaaaaatcc aagacaaaaa
     4081 aagtacacct aaaaaattta aagtaaatcc cataaaaaaa tcaatgttaa cgctttcttt
     4141 attagctata ctcggtgtat caattgaaat gggggataaa aaatgaaaaa caaatttaga
     4201 aagattgttg ggactatcgc tattttgacc gcaagcattc tattaagtgc gtgtggcaat
     4261 tcaggatctg cagatgatag tacggggtat gtaggaatcg ctatgccgac caaatcagca
     4321 gaaagatgga tcgctgatgg gaacaacatg gtctcggaat tagaaaaact gggttataaa
     4381 acagatcttc aatatggaga agataaagta gaaaatcaag tagcacaaat agaaaatatg
     4441 attacaaaag gggtagatac acttgtgatt gcttctatcg atggttcggc tttaactgat
     4501 gtgttagcta aagcaaaaga agcagatatc aaggtgatcg cctatgatcg tttactgatg
     4561 aattcagaaa atgtcgatta ttacgcgact tttgataatt ttggagtagg tgtatcgcaa
     4621 gcagcttata ttgaagagca cttaggattg aaagaaggaa aaggaccatt cacgattgaa
     4681 ttatttggtg gctcgcctga cgacaacaac gcactaatca attacaacgg agtgatgtct
     4741 gttttacaac catatatgga caacggtcag ttggtcgtgc catctggaca gacgagtttc
     4801 agtcaaatcg ctacgcttcg ctgggatggt tctacggcgc aagcaaggat ggataatctg
     4861 ttaagcgcaa attatactga ccaaacattg gatgctgttt tgtcgccata tgatccaata
     4921 agtttaggaa tcatttcgtc attaaaaggc gtggggtatg ggagtgaaag taaaccttta
     4981 cctgtgatta ctggacaaga tgcgacagtt gctggcgtta aatcgatcat tgccggggaa
     5041 caaacacaaa cgatctttaa agacacacgt atcttagcaa aaaatacgat tgaaatgatc
     5101 aaagcaatca gtgatggaga agaggttccg gtcaatgata ctgaaactta tgataatggg
     5161 gtgaaaactg tccctacgta tttagcaaat acagtatctg tcgataaaga taattatcaa
     5221 gcagagctga tcgacaccga ttattataaa gaatccgatc tgaaaaatta agaggaacca
     5281 aacaaggaac taaagagatc taagtcagag agaggataag aattatggcg gattgtattt
     5341 taaaaatggc agatatcacc aaaaaatttt ctggaatccg agcgttagat tgcgtgaatc
     5401 tgatgattga acgtggggaa atccatgcat tgtgcggaga aaacggagcg ggaaaatcaa
     5461 cattgatgaa tgtcttgagc ggcttgtatc catatggctc ctatgacggg gaaatctttt
     5521 atgatggaaa gctgtgtcaa tttaaagatt tgaaagatag tgaagaaaaa ggcatcgtca
     5581 tcattcatca agaattagca ctaagtcctt atctttcaat aaaagagaat atttttctcg
     5641 gaaatgaaca agcagaaaga ggcgtgattg attgggaact gacagaaaag aaaacatatg
     5701 aactgctgaa taaagtcggt ttgtctatcg acccgaatct tctagtatcc cagattggcg
     5761 tagggcaaca gcaactagtt gaaatagcta aagcattatc aaaatccgtc cgtctgttga
     5821 ttttggatga gcctaccgct gcattgaatg aagaagaaag cgcgaatttg ctgagattga
     5881 tccgagagtt gaaagagcaa ggtgtaacct ccatcattat ttcacataag ctgaatgaaa
     5941 tcgtggatat cgcagatcga atcaccattt tacgggatgg acagacgatt gagacactag
     6001 aaaatgaaat gatcagcgaa gagcatatta ttcgcggaat ggtgggcaga gatttagttg
     6061 atcgttatcc tgaaagaaaa ccggatattg gagaagttta ttttaaagta gaagactgga
     6121 cggtctatca ccctcttgat cacgagcgaa tcataaacga ccacatcaac ttgcatttga
     6181 aaaaaggaga gattgtagga atagctggac tgatgggagc gggcagaact gaattcgcta
     6241 tgagtctatt tggacattct tatggaagca agatatccgg acgtatatta aaggaagggg
     6301 cagagatatc agttaagacc gttccttcag ccattgcaaa cggactcgct tatgtctcgg
     6361 aggaccgaaa atctttagga ctgaatctgc tgatggatat tcgggaaaac gtgacgctga
     6421 gcagtttgga taaaataagt caaaaaggag tattagataa agaaaaagaa gtaactgagg
     6481 cagaaaattt ccggaaaaag atgcgtataa aaacaaactc catccatcaa attgtcagta
     6541 gtcttagtgg tgggaatcaa caaaaagtcg ttttatccaa gtggctgatg acacaacccg
     6601 atattctttt tttggatgag ccaacccgtg ggatagatgt tggggcaaaa tatgagatct
     6661 acacgattat cgaagaaatg gcagcaaatg gcaaagctgt ctgtcttatc tcttctgaac
     6721 ttcctgaaat attagggatg tgtgatcgaa tctatacgat gaacgaagga cgtattacag
     6781 gagaagtctc gcgagaagat gcagatcagg aagtcttgat gaaattaatg acgaaagaag
     6841 aggtgggtta agttggaaca ggcaaaaaat cttacggata aaaaagagaa aaaactaaat
     6901 ttgaaaagta gagcagctga tctttttggt cgctacagca tggtgatcat tttagcggga
     6961 ctacttattg cgtttcaatt gatgacggat ggtattttct ggcgtccatt gaatataact
     7021 aaccttgttc tgcaaaatag ccatatatta gtactagctg cagggatgtt attagttgtg
     7081 ctcttaggtc atgtggattt gtcggtaggg tcagtgatgg cgtttgttgg tgcaattgcg
     7141 ggcatgatga tggtcaatcg ccaaatgaac ccgtggatga cagttatcgc atgtcttgca
     7201 gtaggcgggc tgatcggcgc atggcaaggt ttttgggtag cttatgcagg gataccggcg
     7261 tttatcgtga ctttagcagg attgctgatg tttcgtggac tgacacaagt tgttctagga
     7321 ggccaatctc tagcaccttt tccagctgtt ttccaaaaaa tgtctacggg ttatttacca
     7381 gacttaagcg aaggaagcag ccttcacttc ttgacattga ttttaggaat gatcatcacg
     7441 atcgtgttag ttgttggtca atggcgaaca agaaatcgtc ggaaaaatca tttatttgaa
     7501 gtggaatctc caacagcttt cttggtaaag gcggtattta cagcagtcgt tgtactagga
     7561 ataacttata tctttgcctc ttatcaaggg tatccgatta ttttgatcat tcttggtttg
     7621 atcgttgcag catatgggtt tttgacaaat aaaacagtag caggtcgaca gatttatgcg
     7681 acgggcggaa atcgaaaagc ggctgaacta tcagggatca aaacaaagcg aatcacgttc
     7741 tgggtattcg tcaacatggg agtcatggca gctttagctg gattagtttt agctgctcga
     7801 ttgaatgcgg caacacctca agcaggtaca tcgatggaac tggatgcaat ggcggcagta
     7861 tattttggtg gtgcttcgac ttccggcggc attggaacaa tcatgggtgc gattgtcggt
     7921 ggcttggtca tgggtgtatt gaacaatggc atgtcgattt taggcgtcgg ggtagactgg
     7981 cagcaggcaa tcaaaggatt gatcttgctt ttagccgttg tgctagactt gtataataag
     8041 aaaaaacgaa caacgtagga gaacgccggt tattcagaaa aaagactgtg acaagacatt
     8101 tgtcacagtc tttttttcgc taaaagcgaa cattcattgt ttcgtttgta tagactaaaa
     8161 ataatatatg tttttttacc attcgtataa attctctttt tgctcttgat ttttttgctt
     8221 aattgagtta aattacatag ggcgctaaaa aaagccttga ttaaattaat aatgttcgtg
     8281 ttttgctgga tgtgaacatc aggaggataa tgtaatgaaa gtatttgatt acgaagatat
     8341 tcaattaatt cctaataaat gtatcgtaaa tagccgttct gaatgtgata caacggtcac
     8401 attaggtaaa catacattca aaatgccagt agtgcctgcc aacatgcaga cgattattga
     8461 tgaaacaatc gcagaattct tagcggaaaa tggttatttc tatatcatgc atcggtttga
     8521 tgaagcagct cgaattccgt ttatcaaaaa aatgaaaaag cgtggattga ttacctcgat
     8581 cagtgttggc gtcaagaaag aggaatattc ctttatagag aagttggcag aagaatcgct
     8641 aaatcctgat tatatcacta tcgacatcgc gcatggacat gctaactcag ttattgatat
     8701 gattcagcat attaaaaaat atttgcctga aacttttgtg attgccggta atgttggtac
     8761 accggaagcc gtccgtgaat tggaaaatgc gggcgcagat gcaacaaaag tcgggattgg
     8821 tccaggaaaa gtatgtatta caaaaatcaa gacaggcttt gggactggag gctggcaact
     8881 ggcagctctt agatggtgtg cgaaagcagc tcgcaagccg atcatcgctg acggtgggat
     8941 cagaacacat ggcgatattg caaaatctgt ccgttttggt gcgacaatgg tcatgattgg
     9001 ttcattgttt gccggtcacg aggaatctcc tggcgaaaca aaagtcgaaa atggaatcgt
     9061 gtataaagaa tactttggtt ctgcttcaga attccaaaaa ggcgaaaaac gaaatgttga
     9121 aggaaagaaa atctggatcc aacacaaagg atctttgaaa gatacactag tggaaatgca
     9181 acaagacctt caatcatcta tttcttatgc tggaggaaga gatctggaag ccattcgaaa
     9241 agttgactat gtcatcgtga aaaattcgat ttttaatgga gacacaatct aaaaaataaa
     9301 taaaatatta ggatgttgtg aaaaagttgt cctgcattaa gtaataagaa cagccaaaca
     9361 aaaatagttc tttatatgtt ttgtttggct ggaattatta tcgcagatac aagcttttga
     9421 acatcattat tcagaataag ggactgtgat aagatttttg tcacagtccc ttatgttttg
     9481 caaaaaaagt ttttcttttt tctcgtaaat tcggtaaaat caaaagaaaa aatagacaat
     9541 agaaaagaga agcttatgtg ggaaaaagta gagaaaaaac aaggaccgat ttatcgtcaa
     9601 attatggaac agattatgag agaaatcgaa aacggaagga tcaatcctgg aagtcaactt
     9661 gaatcagaac gaaaactagg gcaacttttt ggtgtgaatc gttccaccgt tgtccatgca
     9721 ttggaagagc ttagggagct tggggtacta acgagcaaac gtggcagcgg atggtttgtt
     9781 aatcagactg aatgggggaa attcgctgtt ccaagaatcg attggcggca aatgatctct
     9841 cctcgttatg agcagacgga ctggtatgag caaaaaataa gagaagcaaa gcagatccaa
     9901 aaaacatcgt ttcttgattt atacgctagc gagatgccag atgagctttt gccgaatttt
     9961 gagtttcctt cttattctct tgaagaaatc ttagcggaag aaaaagaaat ggatctttta
    10021 ggctatactc cgttacgtaa caaaatcaaa cggtatttgg aaagtaaatt tgaattggca
    10081 ttttccccca accagcttct tgtgactggt ggcggtcagc aagcaatctt tttgatttta
    10141 caaacgattc tttcagctgg agaggggata gctgtagagt caccttcttt tttttaccga
    10201 ttacctcttt tcaaagcttc aggaacgcgt ttatttggga taccgatgga tgaagaaggg
    10261 atcgacttag aaaaactgaa agaatcgatc cataaaaata aattgaaagc agtgcttatc
    10321 aacccgaatt ttcaaaaccc tacaggaaaa gtgatgagtc aaaaacgacg agaaaagcta
    10381 gtagaattgt gtcgacagta tcggcttcca attattgaag acgatgtatt tacggatctt
    10441 tcatttctaa accaagaaga agtccttcct atacgaacga ttgatccgga taatgtgtta
    10501 tacgtcggtt cgttatctag ggttttagga aaaacaacga aaatcggttg ggtgatagga
    10561 cctgagactt ttatctcgca actagctcaa gcgcaagaaa tgatggagtt ttcgatgaat
    10621 atccttacac aaatcacagt ggctaatgta tttaatgaag caattgattt gaaaatgcaa
    10681 gatgtaagaa aaaagttgaa agaaaaaagc cgactgataa gcgcttgggg agattcacaa
    10741 tcgtttttca agcttcattc aattttaggt ggttactatg cctggattac ttgggatggg
    10801 aaaaaactag accccgaact agcagaaaaa gtagtagata tgggtcttgg aattgcacca
    10861 agtctacttt ttgggcaaga tataaatgga atcagaatca attacagtag gctggatgaa
    10921 aaccaaatac cgttgtttta ttcaaagatg aaaattctag aaaagtggtt aacagaataa
    10981 tttcacaaag taaatagatt aaagaatata acagcttgga tttaaatttc aagctgtttc
    11041 ttttttttcg gaaaaagaat acgatataaa gaaactaaga aaaaaccatt atttgtacgt
    11101 ccgttcacga tgtgtaaata aaataaatgt ttgcaacaaa actgtataac accaacgttt
    11161 ttaaatagaa acagatggtt gtaagtttga ttagaaaata gaaaaaatgc agatgaaata
    11221 taaaacgctt tccttattgt gattttattc actaatgctg ttaatatgcg gattggcata
    11281 atttcctttc atcgtgaaaa tttgtgcttt tagcttgtat ttctcataaa tccatgataa
    11341 tttattaatg aagacggtta aatagtgata cagttttatt cactgtttca atcgcagaat
    11401 gaagagattt tcttatagat gagagaggaa tgtgtgacaa atggcaaaca aaaaagcacc
    11461 gatcgacttc caagcactgc tagatgaagt caatgcagat ttcccagtat atcaagcact
    11521 tgatcaagat ggaaacgtag ttaacaaaga tttagtacct gatttatccg atgatgaact
    11581 cgttgaatta atgactcata tggtatggtc tcgtgtatta gaccaacgtt caacagcgct
    11641 gaaccgccaa ggacgcctag gcttttttgc accaactgct ggacaagagg caagtcagct
    11701 ggctagtgct tacgcatttg ataaagaaga tgttcttctt ccaggttatc gggatgtacc
    11761 tcaactagta aaacatggtt tacctttatc tcaagctttt ttatggtctc gaggacatgc
    11821 tgcaggtaat tcttatccgg aagaattgaa agctctacca ccacaaatta tcatcggtgc
    11881 acaatatgta caagcagccg gtgttgcatt aggtttgaag aaacgaaaca agaaaaatgt
    11941 cgtcttcact tatactggtg atggcggttc atcacaaggt gatttttacg aagcaatcaa
    12001 tttcgctggc gcttaccatg caaatgccgt attttatatc caaaacaacg ggtatgcgat
    12061 ctctacacct cgtgaaaaac aaactgcagc aaaaacactt gcacaaaaag ctgtcgcagc
    12121 tgggattcct ggtattcaag tcgatggaat ggatccgctt gcagtatata ctgtatctaa
    12181 aatggcacgg gaatgggcga tcagtggaaa tggaccagtc ctaatcgaaa cactgactta
    12241 tcgttatgga ccacatacac tatctggtga cgatccaacc cgctaccgta caaaagacac
    12301 agatgacgat tggcagaaaa aagatccatt gatccgcttc cgtaaatatt tgacggaaaa
    12361 aggactttgg tctgaagaaa aagaagaaca agtcatcgaa gcaactaaag aagaaatcaa
    12421 agcagctatt gctgaagccg acaaagtacc aaaacaaaag gtttcagatt tcttgaaaaa
    12481 catgttcgaa gtatctccac aatcaatcaa agaacaaatt gcaatttatg aagcgaagga
    12541 gtcgaaataa tcatggcaca aaaaacaatg atccaagcaa tcacagatgc cttagcactt
    12601 gaactagaaa atgacgagaa tgtcgttgtt tttggtgaag atgttgggaa aaacggagga
    12661 gttttccggg caactgaagg attgcaggaa aagttcggag aagaccgagt attcgatact
    12721 cctttagctg aatctggaat cgcaggattg agtttcggtc ttgcgttaga aggatttcgt
    12781 ccagttcctg aaatccaatt ctttggtttt attttcgaag caatggacga agtagtagct
    12841 caaatggctc gtacaagata tcgaatgagc ggcactagaa atctacccat cacaattcgt
    12901 tcgccatttg gtggcggtgt tcatacacca gaattgcatt cggataattt agaaggtttg
    12961 atcgctcagt ctcctggtat tcgagtagtg attccttcca acccttatga tgcaaaagga
    13021 ctattgattt cttctattag aagtaacgat ccggttgttt tcctagaaca catgaaacta
    13081 tatcgctcat tccgagaaga agtgccggat gaagcttacg aagttccttt ggataaagca
    13141 gcagttactc gtgagggaac agatgtatct attatcacat acggagcaat ggtgcgtgaa
    13201 gcaatcaaag cagcagacaa tcttgcaaaa gaaaatatat ctgttgaaat cattgatctt
    13261 cgtacagttg cgccattaga tgtagaaacg atcattcaat ccgtggaaaa aactggtcgc
    13321 gtagttgttg tccaagaagc acaacgacaa gcgggcgttg ccgcgcaagt agtttctgag
    13381 atctcagaac gcgctatcct ttcattagaa gccccaatcg gacgagtttc tgcacctgat
    13441 acagtcttcc catttggaca agcagaaaat gtctggttgc caaatgcaaa agatatcgaa
    13501 gacaaagtaa aagaaatcgc ggaattttaa gaaaagaagg gacgaaacaa agtcatggct
    13561 tatcaattta aatt
//
LOCUS       DAHHZJ010000064        13428 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.065, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000064 DAHHZJ010000000
VERSION     DAHHZJ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.065"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(<1..913)
                     /locus_tag="NBL02_001510"
     CDS             complement(<1..913)
                     /locus_tag="NBL02_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HCD2572198.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1341..1904
                     /gene="efp"
                     /locus_tag="NBL02_001511"
     CDS             1341..1904
                     /gene="efp"
                     /locus_tag="NBL02_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCD2572199.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2005..2175
                     /locus_tag="NBL02_001512"
     CDS             2005..2175
                     /locus_tag="NBL02_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572200.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            2390..3649
                     /gene="lysA"
                     /locus_tag="NBL02_001513"
     CDS             2390..3649
                     /gene="lysA"
                     /locus_tag="NBL02_001513"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCD2572201.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3636..4436
                     /gene="dapF"
                     /locus_tag="NBL02_001514"
     CDS             3636..4436
                     /gene="dapF"
                     /locus_tag="NBL02_001514"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCD2572202.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4439..5320
                     /locus_tag="NBL02_001515"
     CDS             4439..5320
                     /locus_tag="NBL02_001515"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCD2572203.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5317..6033
                     /locus_tag="NBL02_001516"
     CDS             5317..6033
                     /locus_tag="NBL02_001516"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCD2572204.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6039..6734
                     /gene="dapD"
                     /locus_tag="NBL02_001517"
     CDS             6039..6734
                     /gene="dapD"
                     /locus_tag="NBL02_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HCD2572205.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6750..7751
                     /locus_tag="NBL02_001518"
     CDS             6750..7751
                     /locus_tag="NBL02_001518"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCD2572206.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7748..9109
                     /locus_tag="NBL02_001519"
     CDS             7748..9109
                     /locus_tag="NBL02_001519"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HCD2572207.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9069..10220
                     /locus_tag="NBL02_001520"
     CDS             9069..10220
                     /locus_tag="NBL02_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCD2572208.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10195..11364
                     /locus_tag="NBL02_001521"
     CDS             10195..11364
                     /locus_tag="NBL02_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCD2572209.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11539..12918
                     /locus_tag="NBL02_001522"
     CDS             11539..12918
                     /locus_tag="NBL02_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCD2572210.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13199..13428
                     /locus_tag="NBL02_001523"
                     /pseudo
     CDS             13199..13428
                     /locus_tag="NBL02_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4483 a         2339 c         2924 g         3682 t
ORIGIN      
        1 agtaaatgtt ttttgataaa tagcggtctg ctgcgtctgg aaaaatcgta acgacacgat
       61 gaccaggtgg taaacgacga atttctctta atgctgctgc aaaagccgca ccactcgaac
      121 ttccaactaa tagtccttgt tctcgtgcta actgcctagt atagttaaac ccttctacat
      181 cagagattgt ctcgatctga ttgattgaaa ggggagaaag aaacggcgga atgaattcta
      241 caccgatacc ctcaatttcg tgaggggctg gatcaccacc atttaacaca gagccctctg
      301 gttctacacc gattattcga atgtcgggat atttttcttt caaaaaggta gaagttccag
      361 caaaggttcc accactccca attcctgcta caaaactatg gatattttct ttgatttcat
      421 gaaagatttc tggccctaac gtatgatagt aagcagcagg attatcttta ttttcaaatt
      481 gaagaggcag atagctgttt gatatctcct ctgcaagttt tttacttttg ctgatggcac
      541 cgatgattcc ttgctcacta ggagaatgga tgatctttgc tcccaatgct ttcatcaatt
      601 gttgtttttc taagctaaac ttctctggaa caacaaaaac cgttcttaat ttgtatgtga
      661 gtgcggccag agcgatccca attcctgtat ttccggcagt tggttcgatg atcgtcgttt
      721 tttcattgat ttttccttgg gcaaatcctt ctttgatcaa ataactgccc aatctatctt
      781 tgatacttcc tccaggattt aaatattcta gttttgcata aatagcagaa cctttaggca
      841 tagaatagtg ctgattgtca aagcgataaa gcggtgtgga accaatagct tctgtaattg
      901 tcgagataat catgtgggcc tcctaaaaaa agataataaa aaaaggagct gactattaaa
      961 tcagctcctt gaaagacata gagaaaaatt gagtagataa aaagataagt tacctggtac
     1021 tcattctatg aagggcaatt aatagacatg acaaatagta tgctgctttt atgcaccata
     1081 caacagcaac gattcaattg tttttggttg ctgactgtca tgtgtatccc tccaagtaaa
     1141 atttttatta acgatagcat gaaaaaaaat aaaagtcaaa ggcttgaaag aaaaaaatat
     1201 ttcgatagca tcgtttaccc gtgtttattt ttctgacaaa taggcatgag accttaaaag
     1261 atagagaata ctctagtttt ctggccggtt gtctgataca ataaagttga ttatgataaa
     1321 gaaaagaagg agcaaaaata atgatctcag caagtgattt aagagccggt atgactttcg
     1381 tacaagacgg caaactgatt aaagttctgg atgcaagcca ccataaacct ggtaaaggaa
     1441 atacagttat gcgtatgaaa ctgaaagatg tccgttcagg tgcaacatac gacacaacgt
     1501 tcagaccaga agaaaaattt gaaaaagcac atatcgacac aaaacctgta caatatttgt
     1561 acacgatgga tgatacagct ttcttcatgg atttggaaac atatgagcaa tatgaaattc
     1621 ctgtagaaac agtagaagaa gaaatgaaat atatcctaga aaacatggaa gtaaaaatcc
     1681 aatttttcgg tagcgaagtc atcggcgttc aattaccaac aactgtcgta ttacgtgttg
     1741 tggaaacaca accttccatc aaaggtgcaa cagtcactgg ttcaggtaag ccagcgacaa
     1801 tggaaactgg cttagtagtc aatgttcctg acttcatcga aacagatgaa ttgttggaaa
     1861 tcaatacagc agaaggttct tatgtaaaac gtgcgtcaaa ataattcgct aacggattga
     1921 taagaatttg aaaacagagc ttgacctctt gaaaggcaga tcatcggatc tgcctttcaa
     1981 gaggtttttt gtggaatcag caaattgaaa aataaaaaag tatttgattt tatttatatt
     2041 ttgaaagatg aatggcaaaa gctagagaaa gaaaaaactg cacttattta tgaagaaatt
     2101 gaaatgattc ataaaattga aaatatccga actattccga actatattga agcgaaaata
     2161 ttttttcatc actaaatagt gttgaattta tgtttttatg tataaatagc ttctaatagt
     2221 tcgggtattt tgtttttata accattttta ttcttatttt taaaaaaata tttcgaaata
     2281 tactagacaa ttcagaaaat tttgtttaat attattccaa taaaattcaa atctttatca
     2341 aatgaaattc caataaaaac ggaaaaaaag tgaaaagagg aatgaaaaaa tgacagaatt
     2401 gataattggc ggagtcacag caagcacatt ggcagaaaag tataagacac ctctgtatgt
     2461 gtatgatgaa gaaaagatga gacaaacgat gctgtcattc aagcgaggct ttgtatcgaa
     2521 acgttttgat acaaaagtgc tttacgcctc taaagcattc caaacgattg agatgctgca
     2581 tttagttcgt gaatatggat tcgggctaga tgttgtcagt ggtggggaaa tctataccgc
     2641 tttgcaagct gattttccta gagatactat ctacttccat ggtaacaaca agacgccaga
     2701 ggaactgatc tatgcacttg aaaatgattt gttgcatatt gttgccgata atttgatgga
     2761 agttgaatta ttagcagaat taagccaacg ctatttaaaa aaaatctgta tcatgcttcg
     2821 gttgaatgtc ggagtagaag cacatactca tgaatatatc gtcactgcgc atattgattc
     2881 aaaattcggc atgagctatg aaagtaaaga gtgccaacaa gcccttcagt tgataaaaga
     2941 aagcagctat ctagagttag aaggatttca tgctcatatc gggtcacaaa tttttgatgt
     3001 tactgcttgg ttagctgaaa tcgacaaatt gacgaattac ttaaccgatt ttgaccaacc
     3061 tctttccttg aatcttggcg gagggtttgg gattcgttat acacaaaatg atcagccgat
     3121 cccagtcgaa gaggcactaa aaaaactgat tgaatatacg gaagaagctt tggaaaaacg
     3181 aaaactgacc attcgtcaat tactgatcga accgggtaga agtattgttg gagaagctgg
     3241 tacaacacta tacacgattg gtttcatcaa aaagacacca cataaaaaat attactttgt
     3301 agatggcggg atgacagata atatccgtcc agctctgtat caggcagaat acgattgtga
     3361 tttagcagca aaactgtcgg aagaaaaaac agagaaagtg acgattgcag gaaaaatgtg
     3421 tgaatcaggc gatgtggtaa ttaaagaagc ttatttgccg aaagcagaat cgggtgatct
     3481 tttagctgtt tattccacgg gagcttatgg atactcgatg agcagcaact acaatcgagc
     3541 gacacgacca gcagttgttt ttgtcaataa tggccaatca agactgattg ttcgcagaca
     3601 agcattttct gatctactaa ggggggaagt ttcctatgat ccttcataaa tataatggct
     3661 gcggaaatga ttttatctta ttagattacg cagaagacac agattatagt caattagccg
     3721 cttctttatg cacaaaagaa aaatatgata tagacggatt gatcgccgta aaaatcgatc
     3781 cgttggaaat gatctattac aacaaagatg gcagccgcgc gccgatgtgc ggaaatggca
     3841 ttcgctgttt tgctcgatac gtaaatgatc agaacatgat gaatgaacag gaattcaacg
     3901 tagagacatt agcaggcaca agaaaaatta cggtgttgga tccctcacct ttttattgca
     3961 gcgtagatat gggagtgcca gatgattctc cggaaaaaat caaagctaat caaacttatc
     4021 caataaaaaa ccaggaaata gagatcaatg gtgaaaaagt aacgatcact agtttatttt
     4081 taggaacgat tcataccgtt gtttttgttg aaaatgcgcg tgaggaagtc tggagaaaaa
     4141 gaggtgaact gatctgtcat catcctttgt tcaaagaaaa aaccaatgtc aactttgtag
     4201 aagtagtcaa tgaacaagaa ttgatcgtcc gtacctatga aagaggagta ggttggacac
     4261 ttgcttgcgg tacaggttgc tgtgcctctt atgtggttgc taaggaaact gggaaaattt
     4321 cagcagatca ggtagttgtt cacttggaac aaggagactt gatgatctct ggtgaggatg
     4381 cgatacaaat ggctggccca gcagttcatg aatggacaag aatattggag gaatgaagat
     4441 gataaaaggt tcgatcgtgg cgctgatcac gccgatgaat gaagagggat cagtagatta
     4501 tgctggtttg gaaaaattga tccaattcca tttagatgaa caaacagatg gattattagt
     4561 tttaggtaca acaggagaat cttctacgct cacacaatct gaggaggagc agatccttca
     4621 actgacagta aagaaagtgg ctggccgtgt gccggtgatt gcaggagctg gaacgaataa
     4681 tacgaaagaa acgattgaaa aagcgaagca ttttgcttct ttgggggcag acgcactttt
     4741 ggtcatcacc ccttactaca acaaaacaag tgatgcagga ctagctgctc attttacagc
     4801 aattgcagaa gcctcaccga ttccgcttat tttgtataat gttccttcta gaacaggcat
     4861 gtcgattcca atccatgtat tagtaaattt agcagaacat cctaatatca ttggtttgaa
     4921 agaagcctca ggagatatgg cttatgtgat ggatgccgct cggttgattg gagaagaatt
     4981 cttcctttac agtggaaatg atgatttgat cctaccagta atgagtgtag gaggaagcgg
     5041 tgtaataagt gtatgggcga atatccaacc taaaatagtc catgaattag taaaagatac
     5101 gcaggacgga agatggcagc aagcaaaaga aaagcaatta aatgcacttg aactgatcca
     5161 tgccttgttc agtgagacca atcctattcc tgtcaaagca gccatgtcat tattagactt
     5221 accttccgga cctctacgcc tgcctttagt ttctctttca aaagagaaaa agaaacaatt
     5281 agctcagctt ttgttaaaag aaaggacggt tgaaaaatga aaatcggact gattggcagt
     5341 ggacagatgg gaaatagagt cgaagaagtt gcacaagaaa gaggagatac ggtccttctt
     5401 ttacggacgg cacctaaaga agcaactacg cttgctttta cggaactgcc agaactgctt
     5461 attgattttt ctcatccaga taatttagag aaaatccttt cgtatagctt atcatatcaa
     5521 cttccgttag tcattggcac gacaggttat acacaatcac agttccaaga aataaaagaa
     5581 catgccaaac atgtacctgt gatgtatagt gctaattttt cgttcggcat catggtgatg
     5641 aatcaactaa tcaagcaagc agctgcaatg ttgcaaggct ggcagatcga actgattgaa
     5701 aaacatcata gtcgtaaaaa agatgcacca tctggtactg caaatatgct gcttcaaaca
     5761 atccaagaag tgcaaaagct gcagcccgta tacaactggc aagggaaaaa aagagaagaa
     5821 aaccaaattg gtgtacacag tattcgtgca ggttctttac ctggtgaaca tgatgtattg
     5881 tttgctgcta ctgatgaaat atttacgatc aaacatgaat ctttttctaa tcgaatcttt
     5941 gctgcaggcg cagtagaagc agcggactgg ttgaaagatc agtcaccagg ttattacaaa
     6001 ttagaagatt tattaatgga caaaggggtg taaaaaacgt ggaagctcaa gaaatcatcc
     6061 gctacattca aacggcatct aaaaagacgc ctgtgaaagt ctatctgaat gtaaccgagc
     6121 caatcgaatt taaagaaagt aaagtttttg gtgaaggaag cagcttcgtt gtttttggtg
     6181 actatgagac gattgctccg gttttagaag ctgaatcaga aaaaatcatt gaaatcgaaa
     6241 tcgaaactgc tgcaagatac agcgcagttc ctttgctaga tattaaaaaa attaatgctc
     6301 gtatcgaacc aggtgctatt atcagagatc aagtgtcaat cggcaacaat gccgtgatca
     6361 tgatgggagc gattatcaat attggtgcag taatcggtga aaacacgatg attgatatgg
     6421 gtgctgtctt aggtggacga gcgacagtgg gcaaaaactg ccatattggt gcaggagctg
     6481 tcttagcagg tgtgatcgaa ccagcatcgg ccaaaccggt tatcgtagaa gacggcgtac
     6541 tggtaggtgc gaatgcagtg attgttgaag gtgtccatat cggaaaagat gcagtcgtgg
     6601 cagcaggagc agtggtttta gaagatgtgg cagcagaaac tgttgtcggt ggaatacctg
     6661 cacgtgtatt aaaaataagt gatgatcaga caaaagaaaa tacagcattg attgctgcgt
     6721 tacgcgaatt atagaggaga tggagaaaaa tgcgcgaaat agatacctgt attgtggggg
     6781 caaccggatt agtcggccaa acgatgctca aagtgttaga agaatatgat tttccagtag
     6841 gacgattgaa attattagct tcaaaacgtt cagccggtaa agtttgtgta ttcaagaaaa
     6901 agaaacacac tgtacaggaa ttgacagaaa cttcatttga aggatatgat ttagcgctat
     6961 tttctgcagg tgcttctgta tccaaagagt ttgcgccaat cgcacgaagt cagggactat
     7021 ttgtcataga taattcctca gcatggcggg aagattcgga tattccatta atcgttccag
     7081 aggtcaatct ggaagactat acattgaatc ggctgattgc aaatccaaat tgttcaacca
     7141 ttcaagcggt acttccgctt aaagctttac aagaagcctt tggtatccag cgagtcaatt
     7201 attcgaccta tcaagcagtt tctggttccg ggcaaaaagg cattgaagat cttcatcgta
     7261 cgcaaaatga cgaggaacca gttttgtatc cttataatat tagccaaacg gtgattccgg
     7321 aaatcgatca gcctttggaa aatgggtata caaaagaaga acagaaaatg ataaatgaga
     7381 cccgtaagat tctgcatcag cctgctttgc cagtatcagc aacctgcgta cgtgtgccaa
     7441 tcgaaaatgg acatggagtc tctgtggcag ttcaactgca aaaagagttt acgacggaag
     7501 aaattagaga gtgtctcagc cagtttccag ggattcaatt agaagatgat ctagcagcac
     7561 ataaatatcc gacgagcatt cttgctagag gaacagatat ggtttatgtt gggaggatca
     7621 gaaaagacac ttctttagag aacggattgt tgttctatac gactgccgat aacatacgca
     7681 aaggagccgc tgcgaatgcc gtacagattg ctgcagcttt aatgcagaaa atagcggagg
     7741 tggcaagatg agtaatacgc tgaaagtagc gaaattcggc ggaacatcga tgtctcatga
     7801 agagcaattc aaaaaagtcc gtacaattgt tctttcagat cccaatcgcc aagtgatcgt
     7861 tgtttctgct ttaggcaaac gtacgtcaga tgatgacaaa gtaacagact tactttattt
     7921 gatttatgct taccttcagc atcatgttga ttggacaccc ctttggaaaa ggatttgtga
     7981 tcgtttcatt caagtaagag atgttcttgc cctgcagtta cctattgaag atgaactagc
     8041 acgcatacag aaacaactgg aagacgaaaa gataactgaa gattatttag taagtagagg
     8101 tgaatatctg actgctcaac tgatggctga gtatcttggt taccagttta ttgacgcaaa
     8161 agacctgatt ttctttgact atgatgggaa aatcgatcag gaaaaaacag cacaggaaat
     8221 cacaaaagca tttaaaagat atccgaaaat ggttgttcca gggttttacg gggcgaaccc
     8281 tgcaggaaaa gaaaaattat tgggccgtgg gggaagcgac attaccggcg cgatactggc
     8341 gaagatgttg caagcggaaa gttatgagaa ttggacggat gtttcaggaa tcatgatggc
     8401 tgatccgcga atcatcgatc atcctaaaat catcgaggaa atctctttta gagaactgag
     8461 agagatggct tatatggggg ctaacgttct tcatgaagca gctgttttcc cagttcaaga
     8521 ggctgatatc cccatccaaa taaaaaatac caatgatcct ttcgctcaag ggacaaaaat
     8581 atcgaatcac gaaatagaaa aggaaaatgg actgacagga atagctggac gcaaggattt
     8641 tctttccatc actttattca aacgtcatat gtccgatgaa atcgggttta tctggaaagc
     8701 aatgagcatt tttgctaaac atgagatatc aatcgaacat attccttcag ggatcgacaa
     8761 tatcggagtg atcgtctcag cagaagcgat tgccgataag ttatttctca tcacgaaaga
     8821 attaaaagaa acacttggcg tggaagaaat cgtcgtgatc gaagacttag ctttgatctc
     8881 tgtcgttggg ggtccgcata aagaattgat tggattatct gggaaagtgt tatcgatatt
     8941 gaacaaatta gaaattcgca catcgatctt atcacaagga gcacaggagt tgaatctgat
     9001 catcggtgta ccgaataatc aatatgaaac agtagtgaag ggtatctatg aagggatggt
     9061 gaatacggat gctgaaagaa tatcgaatgg cactccatca aattcctgaa atcgggtttc
     9121 atgaattcaa gacaaaaaaa tatttatatg aacaagtaaa aaacagcgag gccgttatcc
     9181 atgaaatcgg cgaaacagga ctactccttt attttgataa tcagcaagaa gaaacgattg
     9241 cttttcgaac agatatcgat gcactgccag tcaaagaaga aacgaatctc tcttttgctt
     9301 ctaagcatga aggttatatg catgcatgtg gtcatgacgg acatatggcg atgcttttag
     9361 ggttgactga ctttataact aaacacattg aggatcttat ttataatgta gtactgatct
     9421 atcagccatc agaagaaatt tccggtggcg cacaaagcgt tattcagtca ggattgttgg
     9481 ataaatatga agtacaagca atatttggct ttcatctatg gcctggacta gctaagggag
     9541 aagttttctc tcgaccagga cctttgatgg cacaaagcag tgaaacagac attatcatta
     9601 aaggccagtc tgcacatatt gcttctagtg aaaaaggaat cgatagtctg gaggcagctg
     9661 tacgattttt gaagaatgtt tatgattttg accggcaatt accggcagag tggaatcatc
     9721 taatgaaatt tggccaaatc caaggtggaa cgatccgaaa tgttttagct ggagaagtta
     9781 ttgtatccgg aagtatccgt tcttacagtt cagatatgca aaattatttg aagaggggat
     9841 tagaccattt agcagaacag tttcactcta cagctactat ttcttttcgc tataatgacg
     9901 gctatccagc tgtaatcaac catagaggct tatatgcagc actgttagat tcaaacaaac
     9961 tacatgaatt agatgctcca gttcttcaag ccgaagattt tggcatatat acgcaacaat
    10021 atccatgtgt atttttcttt ttaggtgtcg gtgatacccc tgctttacat gatccgacgt
    10081 tcgattttga tatggatgtt ttagaaaatg gtgtagaatg gtacaaaacg attcttttta
    10141 cagaaaatct tcttgtaaaa atcgatagca gttcaaaaga ggagcgagta aataatgatg
    10201 aatttatcca atcgatttag tcctaggctt tctagaatag aagtatcaaa aatacgacaa
    10261 ttcgatcagc aaatatcgtc gataccagat gtgatcaaac tcaccctggg agaaccggat
    10321 tttccaactc ctgaacatgt caagcaagca ggaatagcag cgattgaaga agacttttct
    10381 cactataccg gaatgagagg attagaggaa ctgcgggaag cggcttgtat atttcagcaa
    10441 cagcgttatg gattaactta tgatccgcaa acggaagttt tgacgacagt tggtgcaacg
    10501 gaagcgattg cctctgcgtt gttatctgtt ttggaagaag gagacaaggt tcttattcca
    10561 gcgcctgctt attcaggata tcagccatta gtggaactag ctggtgctga attgatcccg
    10621 atagatactt ctgacacagg gtttgtctgt cagccggaac aatttgaacg agcatttgaa
    10681 caatatggat cagctgtcaa agcagtcatt ttaaactatc ctaacaatcc tactgggacg
    10741 acattgtctg caaaacaatt gaaagcaatc gctgaggttt taaaaaaata tccggtattt
    10801 gttataagtg atgaagtata tgctgaattg acttatagtg gaacgcatat gtccatagct
    10861 acttatcttc cagaacaaac aatcgtgata agcgggttat ccaagtcaca tgcgatgact
    10921 ggttggcgag tgggctttat ttttgctcaa aaagccctta ttgacgaact aattaaagtc
    10981 catcaatatc tggtgactgc cgctacaacg atgtcgcaaa aagctgcggc tgaagcgcta
    11041 ataaatgggg tgaaagatag cgagaatatg aaagagcaat atcaaacacg aagagactat
    11101 ctggtccagc aattggcgcc tttaggattt gaagtgaccc agccaaacgg tgcattttac
    11161 ttgttttgca aactcccagc tgccattcaa actgactctt ggcagttttg tgtagaatta
    11221 gcggaacagg caaaagttgc gtgtatccca ggaattgcat ttggcccaga aggagaaggt
    11281 tatatccgga tttcttatgc tagcagcatg gaaaaattac atgaggcctg caaacggatc
    11341 aaagactttt taacagaaca gtgattcata gattttatca aaaaaacaat cgttaatttt
    11401 gcggtatatt ctgcaaggag acgattgttt tttaaattta gttcaaatga acgcgtttct
    11461 tttgattcat aactaatgta atgttaagtt taaattgttt agaatgattt taaaagaata
    11521 ttcaaggagg tttcatcaat ggaaaaaatc aaagttgtca ttgtaggtgc atctcatgga
    11581 gggcatcaat cgatcttgga actattgaca cgatacggtg aaaatgtaga tatcacgcta
    11641 tttgaagcag gagactatat ttccttcatg tcatgtggta tggagctgta tttagaagac
    11701 aaggtaacag atgtaaatga tgtacgtaat ttcagaccag aaaactttcc gcaatccaat
    11761 gttcatattt taaataatca cgaagtaaaa acgatcgatg ccgataaaaa aacagtaacg
    11821 gttactagcg catcagataa cgaaacaaaa gaatatcctt atgacaaatt gattttgagt
    11881 tctggtgtca aaccaaacaa tctgccagta ccaggagcag atctagaaaa tgtctacttg
    11941 atgcgtggat acaattgggc aaccaaaatc aaagaaaaat taaatgatcc ggctattaaa
    12001 aaaatcgctg tcgtaggtgc gggttacatt gggatcgaag cagcagaagc aagccgaaaa
    12061 gctgggaaag aagtcgtatt gcttgatgtg atcgatagac ctttaggcac ttatttagat
    12121 gctgagatga cagatatttt agaacaacac ctaaatgaaa acggtattga agttttgaca
    12181 aatgcaaaaa tcgaagcttt tactggtaac ggaaaagttg aggcaatcaa aacaagtgaa
    12241 aaagaaatcc cagcagatct agtcattcaa gctgccggtg tgaaggcaaa tacagaatgg
    12301 ttaaaaggga tcgtcgactt agatgaacgt ggctggatcc aaacaaatga atacttgcaa
    12361 acgaatcttc cagatgtgta tgcagtagga gatgctacac ttgcttattc tatcccagca
    12421 cgcaaaaaaa tgccgatcgc tttagcgact gttgcacgta gagaagcacg ttatgtagta
    12481 aaacatcttt ttgaagaagt accaaacaaa ccgttcggcg gagtcgtagg ttcctctgca
    12541 ttaagtgtct ttgactatca ttttgcagca agcggattga atagctttac agcagcaaaa
    12601 gcaggagtag atgtacgagt gtcttattat gaagatacgt tacgaccaaa atatgtacca
    12661 gaagaacaag gaaatccaaa agtgtccatc caattattct cggatccaat gacccatcaa
    12721 ttgctaggag gagcagtatt atctacctat gatgttacag gtcaaggaaa tgtattggca
    12781 ctagcgatcc aaaacaaact gactttagaa gaactagcag aagctgactt cttcttccag
    12841 ccaggctttg accgacaatg gagcatgttg aatctagccg ctcaacaagc gttaggagaa
    12901 gaaccatttg tagaataaga aaacgaaata tttgaaaaga ggttgtgaaa aagctgcctt
    12961 gcatcaagta ataagaacag ccaagtaaaa acagcttctt atgtttttaa cttggctgga
    13021 cttattaccg cagatgcaag cttttgaaca ccgtttattt tcaaaaaagg gactgtgaca
    13081 cgatttgtgt accgaccccc aaaagttaga ccaaaaaatc taacttttgg aggtcggtat
    13141 tttttatggt taaatatact tttgaactta aattaaagat cgttcatgat tatttggatg
    13201 gcaaaggcga ttggaccgta aaaatccaga tcaagagatt gaagcggaaa tgcttaaaat
    13261 tcgggagaag cacaaggatt atggctgttt acggatgacc aatgaactaa gaaaccgcgg
    13321 tttctctatc aataaaaaga aagttcagcg cttgatcaag aagctaggaa taaaagtaac
    13381 ggcatacacg cgtaaatcac gtcgctacaa ttcttatcga ggaaaagt
//
LOCUS       DAHHZJ010000065        13399 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.066, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000065 DAHHZJ010000000
VERSION     DAHHZJ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.066"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(<1..159)
                     /locus_tag="NBL02_001524"
     CDS             complement(<1..159)
                     /locus_tag="NBL02_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572211.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENNQ
                     PVIAKKETV"
     gene            776..1438
                     /locus_tag="NBL02_001525"
     CDS             776..1438
                     /locus_tag="NBL02_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572212.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            1517..4744
                     /gene="fss3"
                     /locus_tag="NBL02_001526"
     CDS             1517..4744
                     /gene="fss3"
                     /locus_tag="NBL02_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HCD2572213.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITDAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            4858..5172
                     /locus_tag="NBL02_001527"
     CDS             4858..5172
                     /locus_tag="NBL02_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCD2572214.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            5185..5559
                     /locus_tag="NBL02_001528"
     CDS             5185..5559
                     /locus_tag="NBL02_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCD2572215.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            5569..5913
                     /locus_tag="NBL02_001529"
     CDS             5569..5913
                     /locus_tag="NBL02_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HCD2572216.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            5984..7336
                     /locus_tag="NBL02_001530"
     CDS             5984..7336
                     /locus_tag="NBL02_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HCD2572217.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFWVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            7396..8538
                     /locus_tag="NBL02_001531"
     CDS             7396..8538
                     /locus_tag="NBL02_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HCD2572218.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            8563..8817
                     /locus_tag="NBL02_001532"
     CDS             8563..8817
                     /locus_tag="NBL02_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572219.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            9073..10257
                     /locus_tag="NBL02_001533"
     CDS             9073..10257
                     /locus_tag="NBL02_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCD2572220.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            10254..10391
                     /locus_tag="NBL02_001534"
     CDS             10254..10391
                     /locus_tag="NBL02_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HCD2572221.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            10396..10617
                     /locus_tag="NBL02_001535"
     CDS             10396..10617
                     /locus_tag="NBL02_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572222.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            10630..11130
                     /locus_tag="NBL02_001536"
     CDS             10630..11130
                     /locus_tag="NBL02_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HCD2572223.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            11227..13074
                     /locus_tag="NBL02_001537"
     CDS             11227..13074
                     /locus_tag="NBL02_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HCD2572224.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            13077..>13399
                     /locus_tag="NBL02_001538"
     CDS             13077..>13399
                     /locus_tag="NBL02_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HCD2572225.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNFYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLT"
BASE COUNT         4513 a         2152 c         2971 g         3763 t
ORIGIN      
        1 tactgtttct tttttcgcaa ttacaggttg gttattttcg gaaaaagcta atatagctaa
       61 gattgaaaca tccatctgga atttatcttt tttgctgctt tgctcataaa agtctgcata
      121 ttcatcactg atattttttc taataaagtc ttccatcata aaaatcacct caaaacgatt
      181 atacgaactt acgttctcgt tgtaaagcga tatttgaggg gcaaaaaggg ggcaaaaata
      241 cgtttacctt tgatatatct cagattgaac atagtgttta ttatatgtcg gtattgttga
      301 tttatagctg ttttgagggt attagataca taatagaata tagtggtttt gtttacgtgc
      361 attacacgga aaagctgctc gtatcaaaga aatcagaaga taaacacgga aaacttcctt
      421 ggaaatctga aatttgtgaa aagtccttgt atatcaaggg ctttttcttt tttctctatc
      481 acagttttaa gccatttttg gctgttttgg accatttttc aatcatattc ccttaaaaat
      541 tgtcctagat tgtccgacat tgactatcag tgtcgtcaaa atagggcaaa atttagtagt
      601 cagcttgacg acaaatataa tacgaaaaat accaaaacat ttaccgaatt tagcaacttt
      661 tgaacccaag tttctcaaag ggtcagctat tagcaaaaaa gacaaaacat gtacttttct
      721 actaaaaagt tgatgtgata ggcttctatc taaataacta ttaggagttt ttatcgtgaa
      781 gcaaaatgat ggacgtatca tttggaaaaa tcaaagtgag ttaaaactga ttttaacaat
      841 taatgaattc atcgaaaaac acggaattac atctagccgt caataccaga aaaagctatc
      901 agaaaatcca aattctgcac caagtatgtg gttcattaac aaaaaatatg gatcatggga
      961 gaatttattg attagtatag gacgagaaaa tactgggtat ggaaaatggg ctagaatgtc
     1021 agaacaagaa ttattggaaa tagtagaagc ctttattaag tgcgagaaga taacctctca
     1081 acgaatgtac gaacaaaaat cagtagggaa gaatattcct tcacttagta ccattaaaaa
     1141 gatgttagga gatatacgtc cactttttaa agaaaaaaat gatggatctc gatttactga
     1201 tttcgaactg ctgttagaac taaaaaatga aataatccga ttaaaactac aggatgattt
     1261 atcaatgaca aagtttcgaa aactagttca atccccaaaa ctaccatcag ttgatacaat
     1321 tatgaaaaga acgaacaaaa attgggagga attgatggca gagattggat ttgattatcg
     1381 aagaatcaaa atttataagc agagaaacaa cctatccaag acaaagaaaa ctaaatagta
     1441 tcactctaaa tcagacgttc aaaattaaaa tttggacgtc ttttttatta cccaaaaacg
     1501 aaaggagatt tattttatga aaaagccaag atttaagaat tggcgtttgc tcgcaacact
     1561 gagtctatta tgccaaacaa ttggtggatc gcttggccca acgattgcct ttgctgatga
     1621 aattactcat ccacaaacgg taactgtgga attggatttg gctcaccagt atgctgtcga
     1681 aggtacattt agcgatggtc gtcccatgtc agaagttacc gttccgcact atgcagttta
     1741 caatggtgtg aagcaagata ttttctgtat tgagccaggg gtgccgattt acaatgagtt
     1801 cactcctggt tatgagaaaa atccattgcc tgatatgtcg gagaaagcga aattagtttc
     1861 cgttttatgg aaggaagctg gtacggatgt ggatacacat attgtggccc agaagatgat
     1921 ttggcaagaa gttaatggct acacgcttca ctcaattaaa cgttctgatg gtagtgcagt
     1981 aaatatcgca gccattgagt caaaaatcaa tcaagccatt gcggattatc aaaagaaacc
     2041 aagcttccat aactccacag tgaaaaccgt gttaggtcaa tcgactactg tgacagatac
     2101 gaacggcttg aatttatcgg agtttgatga agttgtcgag aatatcgcaa atattgatta
     2161 tcgtgtgaac ggcaatcaac tagtgattac tccaaatgcc aattcaaaag agagtggtga
     2221 attaacccta aagaagtcag ctaatacagg aacaccggta gcttataaaa tggctggtca
     2281 acaaactttg atggcggggg caattgataa gcccaatacc tacacaatca aaattgatgt
     2341 ggaaactgag ggttctttga agattaaaaa agtcgataaa gaaacaggaa atgttgtacc
     2401 gggaaccgtt ttccatttag actttggaaa aacattacct gcaaaagacg tgaccaccga
     2461 taaagaaggc attgcgacat tggatgagat tcctcatggc gcgaaagtaa ccattactga
     2521 aaaatcagtg ccagctcctt atacgattga cactacacca atcaccgcaa ccattaaatc
     2581 tggtgagact atttctgtga cttcaaaaaa tactcgcgaa aaaggtcaaa tcattttgaa
     2641 taaaatgggg gtggaaacag gaactgatct ttggaacgac aaatactcat tagccggaaa
     2701 tacgtttgcc attcgcaaag acagtccaac cggtgaaatt gtccaagaaa tgaccacgga
     2761 tgaaaaaggt catgcggaaa caccaaaaga gattgccaat gcgttggaat tgggaaccta
     2821 ttatgtgacc gaaaccaaag ctagtaatgg ctttgtgaat acctttaaac cagtaaaagt
     2881 cgaattgaac tatgtcaatc aaaccgtggc tcttgttacc agtaacgtaa aagggcaaaa
     2941 ccaagaaatc actggtgaaa ccactttgac aaaagaagac aaagataccg gtaatgaaac
     3001 tcaaggtaaa gctgagttta aaggagctga atatactctc tttactgcaa aagatggtca
     3061 agcggtcaaa tggagtgagg cttttaaacc agagttagtg aagggaacga aagcttctga
     3121 tgaaacggtg actttgactt tagatgaaaa gaatcaagtt gctgttaaac acttagcgat
     3181 taatgagtat tactggcaag aaaccaaagc acctgaagga tataccttgg atgaaacaaa
     3241 gtaccctgtt tccatcaaaa aggtggatga taacgaaaaa aatgccgtga ttacccgaga
     3301 tgttacggca aaagaacaag tcattcgctt tggctttgat ttctttaaat ttgctggatc
     3361 agctgctggg actgccgaaa ctggcttcaa tgatctgacc tttaaagtat cgccattgga
     3421 aggaaccagt gagattacag atgccgaaga tgaagcaatt acagcttata acgagcaact
     3481 aggttttgat ggctatggca agtttgaaaa tcttccttat ggggattatt tacttgaaga
     3541 agtagaggct ccagaaggat ttcaaaaaat tacgccatta gaaatccgtt ctacttttaa
     3601 agaaaataaa gcggattaca ctaagagtga atacattttt acgattaccg aaaaaggaca
     3661 aaaacaacca attaaaacgg tgaccgttcc ttacgagaaa ctgacgaata aagcattttc
     3721 tgttagcttg aatcgtttga tgctctatga tttacctgaa gaagaagata gcttaacttc
     3781 tcttgggact tggaaagacg gaggcaaaga attgacagac cttgattcta ccgagctagt
     3841 tgataaattg agctataact tgcatgaaat caaagaagac tggtatgtgg tagcgcaagc
     3901 cattgatgtg gaagcgacaa aagctgccca agaaaaagat gaaaaagcca aaccagtggt
     3961 aattgccgaa acaaccgcaa cgttggcgaa taaagagaaa acaggaactt ggaaaatttt
     4021 gcataaatta accgcagaac aagttttgga taaaaccatc gtcttattca actatgtgta
     4081 tgaaaacaag aaaccctatg aagcaggtga tgatccagta gcgaaagatg ctagcttgaa
     4141 caatcaagcc caaactgtca attgtacggt ggaacgccat gtttccatcc aaacaaaagc
     4201 ccacctagaa gatggttcgc aaacctttac tcatggggat gtggtggata tgttcgatga
     4261 tgtgtcgatt acccatgatg tactggatgg ctcaaaagaa gcttttgaaa caattctgta
     4321 tgcattactt ccggataata cgaccaaaga aatttggaaa tctggcaaaa ttgattatga
     4381 agtgaatgac aaagaattca ctaaaaccgt acttgcggaa aaagtggaca caagtaaata
     4441 tccagaaggt acaagtttta ccttcaaaga aattaactat ggcaaagacg gaaacatcaa
     4501 tgggaaacac aatgaggatc taaaagaaaa aactcaaact ttaacaccta aaaaagttcc
     4561 gacaacacca agtgcaccag aacaaccgga aataccaact gttccaagtg actctcaaga
     4621 atctagtcct acagtaaaga cattcccaca aacaggggag aaaaattcta atgttctgct
     4681 gtttattggg tttaccttga tctttgcgac tgctgggtat tatttctgga atcgccgaaa
     4741 ctaaggtgat gtgatgaaac aactaaatag gaaaaaccaa cgtgcgcgac cgcctcctaa
     4801 aaaaagtggt cgcgctattt ttgtatccaa aactaaaaat gaaaagagga aaagaagatg
     4861 gaattgaaat ttgttgtgcc agatatggcc gaaacctttg gaaagataag gtatgccggc
     4921 gaaggcgaag ttttaactga aggatatggg cggaatacta cagtaattgg tcgtagttat
     4981 catttgtatt ccagtaagca acgagccgat gatattgaag tggtggtagc tgcggaagcc
     5041 ggcgaaaagg attttgatca agatcaacca ctaaaagccg tgaatcccca tttggttgcc
     5101 aaaggctatg agattgagaa tcgtgggttt accgactacg tgttgtatgt cgatgattta
     5161 gtgaaagcat aggaggaaaa gaagatgaga ttaacagaag gtattgttgt agattcagga
     5221 ctaacgtttg ggaaattacg attttccgca ttacgccgag aagtacggaa gcaaaatgag
     5281 gatggaacgg ttagtaacga ggtaaaagaa cggacttata atttgaaatc ctctgcgcaa
     5341 ggtcgaatga tccaagttag tatcccagca aatgtaccct tacgcgagtt tgcctatgac
     5401 gcagaggtag agttggtcaa tccaatcatg gatacggtcg ccaattatgt tttccgagaa
     5461 ggaactactg tcaattggtt cattaaggcc gatgatttag tcttgaaacg acaaccagat
     5521 cagggaaatt ctacaaacca aaatgaagga aagaaatagg tgaagcgtat ggaaattaat
     5581 gttgaacata ttttagattg tctcgatcaa tatggaaaag gtgagctaac ggaggaacaa
     5641 ctcactaaag cattaaccta tgatgaaaaa atgtttctga ttatgcatca agggttactg
     5701 gaagtaggta acgatgccga agaagatttt gatgtactga attggttagg ggaagaagag
     5761 tccttcttta tggttatcga agtaaatgaa agtctatgtc gagaagccga aaccgtattg
     5821 gaagaaattg gtgtggaaat gccggatgcc attgaagttt ttttaaagca actagtggaa
     5881 acgaaacaac tccctatagc agtaaatgac tagcataact gcttgaaatt ggatcaatga
     5941 aatcctatga agcttttttc gattggaggt ggaaaccata aaaatgtaca aagggcatcg
     6001 tatacgggca ggagatcagc acttggttta tcactttgtt cttggctggc tactcgctct
     6061 gttcatcggt tggatgagcg tcttttattt tcaagagctt agacaatttg atatttctaa
     6121 gctatcactt tctactatag aaatcgttcg ttctataaaa gatttaatct atttgctagg
     6181 aagtctcgtt ctttcaggaa gcacgatgtt gctttatata cacttttttc aggatcattg
     6241 gcgcagtttg tggcatcgac aaaagctagc ccggatgatt ctagaaaatc attggtacga
     6301 agtaaaacaa actcagagtg aaggattttt caaagacctc aatagtagtc gaaccaaaga
     6361 gacaatcagt tacttcccca aaatctatta ccgtatgaag gatggtttac tttctattcg
     6421 cgttcaaatt tcactgggaa aatatcaaga tcagctttta aagctggaaa agaagttaga
     6481 aagtggtcta tattgtgaac tagtagaaaa ggaactcaaa gactcctatg tggagtacac
     6541 tttactgtat gatataattg ccaatcgaat tgggatagac gaagtagtgg cagaaagtgg
     6601 ggctttgcga ttgatgaaaa atcaagtatg ggcttatgat tctttacctc atatgttaat
     6661 cgctggcggc acaggtggtg ggaagactta ctttttactc actatcatcg aggctttatt
     6721 aaaatctgat gccgagcttt ttgttcttga tcccaaaaat gcggacttag ccgatttagg
     6781 tacggtaatg cctcatgtct attcccaaaa ggaggaaatt tctgcttgtg tggaagattt
     6841 ctatgagcgc atgatgactc gtagcaaggc aatgaaagaa atgcccaact acaaaacagg
     6901 agagaattat gcgtttcttg gacttccacc aaacttttta atctttgatg aatacgtggt
     6961 ttatatggaa atgttaacga cgaaggaaag tgcggtgatt ttgaataagc tcaaacaaat
     7021 cgtgatgtta ggtcgtcaat ctggtttctt tctgattcta gcttgtcaaa gaccggatgc
     7081 gaaatattta ggagacggga ttcgcgatca atttaatttc tgggtggctt tgggtcgtat
     7141 gagtgaactg ggatattcca tgatgtttgg cgaggttgat aaaaaattct ttatgaagcg
     7201 tatcaaaggt cgaggctatg tagatacggg agggagtgtg attagtgagt tttatacgcc
     7261 acttgtacca aaaggatatg atttcttaag ggaaattagc gacattggtg gattaaacgt
     7321 taatacggaa ggataaaata atagtatgta gcctaggagt gtcaagaatc ctgggctttt
     7381 tttacgaggt gatttatgac atttttagat ttattcgctg ggattggtgg cttttgctta
     7441 ggtatggaac aagcgggtca ccagtgtatc ggcttttgtg aaattgatga ttttgctcgc
     7501 cagagttata aggcaatcca tgatacaagt aaggaggtgg aaatgcatga catcacaagt
     7561 gtatcagacg agtttattca atctctcgga ccagtggata tcctttgcgg tggatttccg
     7621 tgccaagctt tttcaattgc gggaaagcgg caaggatttt ccgatactcg aggtacttta
     7681 ttctttgaaa tcgctcggtt cgccgctatt ctccaaccta agtttttatt ccttgagaac
     7741 gtccggggat tactcaatca cgaaggaggg gctacgttcg agacgatcct ccgaacgctg
     7801 gatggattgg ggtatgatgt ggaatggcaa gtgcttaact caaaggccta cgttccccaa
     7861 aaccgtgagc ggatcttcct tatcggacat tctcgagacg catgtaccga acaagtattt
     7921 cctatcattg gatcgtctcc aacatctgat caaaacatca gaaacttatt aaatatcaac
     7981 ccttccaatc gaggaatggg gggacaagtc tatgggtcag atgacgtagc gcctaccttg
     8041 accggtgatg aaggaatcaa aatcgcattg ccggtagatg atggaatttc tgtagcgggt
     8101 atgttactgg gaaattttga acaaggcaat cgggtttatg agataactgg aacagcgcct
     8161 actttgtcaa cgaaacaagg cggaacgaag atcatgcttc ggaaaaacga aacagaatat
     8221 caagaggtga agccagggaa cagtgtgaac ctggcttttc ctaattccac aagtcgtcga
     8281 ggccgtctgg gaaaacaaag tgtgcatacc ttattaacag gagaccaaca agcagtcgtc
     8341 acggatcagt atcaaattcg aaagttaaca cctagggagt gctggcggct tcagggcttt
     8401 ccggactggg cttttgatcg agcttctcaa gtcaattcag atagtcagtt gtataagcag
     8461 gctggaaatt ctgtaacagt tccggtcatt tttgatattg ctagaagatt aaaggaggta
     8521 aaaacagatg atttatgatg agttgattgg ggaatctact gggtgattgg caagattcag
     8581 tcagatccgg aattggaaga agaactgtac cggctcaatt ttgatattcg caaaaacggg
     8641 gtgaaagtcc ctgatgatcc gtatgtaatt gatgaggaaa cggatgctcg aatggagctg
     8701 aatcaagtca tcagtgaatt ggaacgaatt gcggactttg ctaaagaacc tgatatccgc
     8761 caatacttgt ttgagataaa agcagagttg gaaattgagg gtattaccgc ggagtaacgg
     8821 ccaaataaac cactgatagg cactgaccgc gggggatttg cgaagcagaa atcctccgtg
     8881 gtgctgtgcc tgtttatgga cggcgttagc cggccatgat gtctcacccc ccgtatctaa
     8941 caggggggta caaaaacaca aaaaataagc aagcaacgat aaggaatggc tgtcctatca
     9001 aggataataa gcaatttgtg tagatgttaa ctgatttgaa aaagttaaca ccttaaattt
     9061 ggaggtgaga acttggcaca aaggaattta gattatcggc tattaaaaga ccggcgtaat
     9121 gaatatggcg tttcacaaaa taaattagct acgacttgtg gacttagtcg cccgtactta
     9181 aatcagatag aaaatggtgg cgtaaccgca tcgacaaaaa ccatgaggaa aatttttgac
     9241 caactggaaa gtttcaatcc cgagttacct ttaacgttac tgtttgacta tgtaaggatt
     9301 cgctttccca caacggatgc gaggaaaatc attcaagaga ttctccattt gaaatttgat
     9361 tatatgctcc acgaagatta cgccttttac tcctatcaag aacaatatgt tatgggagat
     9421 attgtggtga tgttgtcaca tgaagaagat aaaggcgtcc ttttagaatt aaagggtcgt
     9481 ggttgtcggc agtttgaaac ttttttactc gcccaaaagc gtagctggta tgactttttc
     9541 gaagattgtc taaaagccgg tggcgtgatg aaacgcttag acctggcaat caatgatcga
     9601 gttggcctgt tagatattcc tgatttaacg aaaaaatgtc agaaggaaga atgtatctcc
     9661 ttgtttcgta ccttcaaaag ctatcgttcc ggggaactgt tgaaacctga tgataaagat
     9721 ggtatgggca atactttgta tattggcagt cttaagagtg aggtctattt ctgtttgtac
     9781 gaaaaagact atgagcaata tatcaagtta gggattcctt tagaccagac cgaaacgaaa
     9841 aatcgttttg aaatccgact gaaaaatgat cgagcttatc atgcaattca agatttgtta
     9901 aaaggtcgca gtattgaaag taccacattt tccattatta accggtactt gcgatttgcg
     9961 gataaggtgg aaggcaaaag gcgaactaac tggcccttga atgaacaatg ggggcgcttc
    10021 attggacgga atcagaagga aattcaatta acttctgaac cgaagcctta taccattgaa
    10081 cgaacgttga actggcttgg acgacaagtc gcccctacgt ggaagatggc caaggagttg
    10141 gatcggttga atcagacaac gtacattcag gatatggtaa ataatactcg actatcagac
    10201 cggcacaaaa agattttgga gcaacaaagt atggcaattg aaaatttgat tatatgaaaa
    10261 gaggaatcgt gatggaaatt gtgaaagata ttttattggt gtttggtggc agtatgcttg
    10321 gtgttactgt gatgtgcttg atgcatgcaa gtgcagcggc ggaccgagca atggaaaaga
    10381 aaggaaagtg atgaaatgaa tttcggtcaa aatttgtatc aatggttttt aaccaatgct
    10441 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
    10501 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
    10561 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
    10621 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
    10681 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
    10741 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
    10801 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
    10861 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
    10921 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
    10981 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
    11041 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
    11101 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
    11161 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
    11221 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
    11281 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
    11341 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
    11401 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
    11461 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
    11521 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
    11581 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
    11641 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
    11701 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
    11761 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
    11821 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
    11881 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
    11941 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
    12001 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
    12061 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
    12121 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
    12181 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
    12241 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
    12301 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
    12361 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
    12421 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
    12481 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
    12541 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
    12601 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
    12661 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
    12721 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
    12781 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
    12841 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
    12901 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
    12961 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
    13021 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt
    13081 tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa
    13141 ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca gcaatgtcaa
    13201 gaatgaataa atcatatcaa aatttctatt ctgagagaag tagttcctcg cgcgaaattt
    13261 tttctgatat tacaggaata gaggctacta ttcaaccaaa tagggatttc aataattatg
    13321 ttaaagagct cagagcttgt attaataagg aggacacttt tgacagtgag tttggatctc
    13381 ttattgataa gctgacaaa
//
LOCUS       DAHHZJ010000066        13226 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.067, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000066 DAHHZJ010000000
VERSION     DAHHZJ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.067"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(237..309)
                     /locus_tag="NBL02_001539"
     tRNA            complement(237..309)
                     /locus_tag="NBL02_001539"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(274..276),aa:Lys,seq:ctt)
     gene            690..1259
                     /locus_tag="NBL02_001540"
     CDS             690..1259
                     /locus_tag="NBL02_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HCD2572226.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1246..2106
                     /locus_tag="NBL02_001541"
     CDS             1246..2106
                     /locus_tag="NBL02_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCD2572227.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2296..3000
                     /locus_tag="NBL02_001542"
     CDS             2296..3000
                     /locus_tag="NBL02_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD2572228.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            3005..4840
                     /gene="walK"
                     /locus_tag="NBL02_001543"
     CDS             3005..4840
                     /gene="walK"
                     /locus_tag="NBL02_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HCD2572229.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4837..6153
                     /locus_tag="NBL02_001544"
     CDS             4837..6153
                     /locus_tag="NBL02_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572230.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            6154..7023
                     /locus_tag="NBL02_001545"
     CDS             6154..7023
                     /locus_tag="NBL02_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HCD2572231.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            7078..7887
                     /locus_tag="NBL02_001546"
     CDS             7078..7887
                     /locus_tag="NBL02_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCD2572232.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7990..8619)
                     /locus_tag="NBL02_001547"
     CDS             complement(7990..8619)
                     /locus_tag="NBL02_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCD2572233.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8789..10081
                     /locus_tag="NBL02_001548"
     CDS             8789..10081
                     /locus_tag="NBL02_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCD2572234.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(10157..10819)
                     /locus_tag="NBL02_001549"
     CDS             complement(10157..10819)
                     /locus_tag="NBL02_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCD2572235.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            11044..11328
                     /gene="groES"
                     /locus_tag="NBL02_001550"
     CDS             11044..11328
                     /gene="groES"
                     /locus_tag="NBL02_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCD2572236.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11379..13004
                     /gene="groL"
                     /locus_tag="NBL02_001551"
     CDS             11379..13004
                     /gene="groL"
                     /locus_tag="NBL02_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCD2572237.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4394 a         2284 c         2849 g         3699 t
ORIGIN      
        1 tttagagagt tcattggcag catgattttg caaagcttct cgaattattg ttatattaga
       61 aactttactg ttcataatta tgcttctttt ttaactcaaa gtccaaaggg ctacagtgga
      121 ctttgagtta aaaataaaaa gccctagtta ttttgaaaaa taaaaaaaga acagcgtaaa
      181 tgttttcgaa atgctttcgc accaacattt acctgttccg tgggtttggc acccaatggg
      241 ccgtgagggg ctcgaacccg cgacccgctg attaagagtc agctgctcta ccaactgagc
      301 taacggcccg tacaaaaagt acttaataat agtagcacta agaattttaa aatgcaagac
      361 tacatttaaa gaaaatcaga aaaatcttta cttccttata ggaaatcatt catagattat
      421 tcacatttgc tcgctattat ataagcatac caacaaacaa agttcttgca agagaacaaa
      481 ccctaacact tttttcattt tttactcctt tcccgccgtc tccccggcgg gtctcctgtt
      541 cttaacgata aaagacaaga aggcttagcc ttcttgtttt ttgttttcat ggaatcccgc
      601 taatcaaatc acctagttaa agcaactaga ttgaatgttt atcataagtc tgatatcatg
      661 tttatgaaat gaaaaaagga gcggttatga tggaaaaaat gcgtcagcaa ttaatggagt
      721 gggggaagga actagaaaca ttccgtctgc ctcactggga agaactccct gacttagaac
      781 tttatatgga ccaagtcaga acgttagttg atcgttatct ttcacctgtg atacaaggag
      841 aaaagcatcc attgcttacc tcttctatgg taaacaatta cgttaaacta ggtttgatac
      901 cagcaccagt taaaaaaaga tataacaaag aacacgtagc atttttactt gcaatcacta
      961 cgttgaaaca agtattgact attcctgaaa tcaaagaagg gatattgttc caaggaaaaa
     1021 cagtaggaat tcgtgaagcc tataatctat tttgcgatga acaagaagca gctgtgtgga
     1081 tggtcagcca attagcacaa ggtaaatctc atccgcaaaa attcgaccag gcaacccctg
     1141 tagaatatat tgctgtaaga gcggcaactt tatcgtttgc aatgaaactt cttgcggaaa
     1201 aaacgattgt tctagaaacc gaatatttaa aagaggagaa aacaaatgaa aaacaataaa
     1261 atcgcaattt tagtagattc aggtacggat gttccagaag aattgataaa aaaatacgag
     1321 atgtatatga tcccattgaa aattatttat aaagatcagg tatatacaga taaaattgac
     1381 attacccctg aagagatcta tcaacgtttg ccagaggaaa tccctggtac atctttgccg
     1441 gatggtgagt cgatcacaga aatctttgag caaatcaaaa aagacggtta tgaaaaggta
     1501 cttgcggtaa caatttccag tggattgagc gggacatata atgtggttcg tttaattgca
     1561 caacagcaag aagaattgga agcatatgta ctagatacga aaaatattgg tattggtgca
     1621 ggtttttctg cgatccaagc agcaaaatgg ctggaagaag gagtagaatg ggatcaactg
     1681 atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt tctttaatgt tgcgacattg
     1741 gaatatcttc aaaaaggtgg acggatcggt ttagttgctt ctattgtagg aaccgcttta
     1801 aagcttaacc caatcatctc atgtaatgaa gaagggattt attacacagt aggaaaagct
     1861 aggggacgta aaaaaagttt ggataaaacg gttgcttacg tcaaagaaaa aatcggtaat
     1921 gcaaaggtct tcaacttagc tgttgcccat ggggatgcga aagaagaagc aattgaaatg
     1981 gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa tctactttgg ccagatttct
     2041 ccggcattag tagtccacac tggtccggga ttattgggtg tcggtgtaca agttttagaa
     2101 ggataaatca aaaaaaggaa acactgaggt gaaaatcagt gttttccttt tttattgaag
     2161 ttagaacaaa aaataataaa gtattttgca ctttcatttt aattttctct tttgttttat
     2221 aggcaaagat gaaacaaaag cagaaatacg gtataatgag aaggtatgac aataatatat
     2281 aagaggtggc tctttatgaa aaaagtttta gttgtcgatg atgagaagcc gatttcggat
     2341 atcgtcaaat tcaatttagc caaagaaggc tatgacgttt atactgcata tgatggggaa
     2401 gaagccttgg aaaaagttgc tgaggtagaa cctgatttga ttttacttga tttgatgcta
     2461 cctaaaatgg acggcttaga agttgctcgt gaggtacgca aaacttatga tatgccgatc
     2521 attatggtaa cagccaaaga ttctgaaatt gataaagtcc taggcttaga gttaggcgcc
     2581 gatgattacg tgaccaagcc attttccaac cgagaattag ttgcacgtgt caaagcaaat
     2641 cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg cagcacctgc ggaattgacg
     2701 attggtgatt tgacgatcca tccagaagct tacatggtga cgaaacgagg agaaacaatc
     2761 gagttaacac atcgtgaatt cgaactactc ttttacttgg cgaagcacct tggacaagtg
     2821 atgacaagag aacatttatt acaaacggtt tggggttatg attattttgg agatgtacga
     2881 acagtggatg taactgtacg tcgtttgcgt gaaaagatcg aagataatcc aagccatcca
     2941 aattatttag tgacacgtcg tggtgtcggt tactatttgc gtaacccaga acaggagtaa
     3001 ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat tttaagatcg ctattacctt
     3061 tattctgatt ttattgatct cgatcgaaat tattggtgcc tactttatac gtggtttaga
     3121 acgttccacg atcaatacat ttattaaaga tatgaatcaa acggtggaat cattagcaac
     3181 aacaatcagc ccggaattga atcgaaaaga taatgcagat gatgaagagg taaatgcgaa
     3241 tatcaaaaga tttattgaaa acagtgcgac tagcgatatc atcgaaatac gggtcgttga
     3301 cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa caaagcgctg tgggaaagaa
     3361 aaatgattat gttgatatca atgatttcac gactaaacgt tatgttgctt tagataacga
     3421 cgataaacgg gtgaatatca atgttcaacc gatcctgtcc ccgaccggcg atgccgttat
     3481 cggggctctt tacgtcaaaa gtaatatcga acaaaaatat tcggaaatca ataacacagc
     3541 agtcattttc tttactgcct cactaattgc ggcatttatt tcaatgatcg tatctgtttt
     3601 agttgctcgt tcgattacac agccgatcgg tgaaatgaga gaacaagcca ttcgaatcgc
     3661 tagaggagac tacagtcgga aagtcaaagt gcatggacaa gatgaacttg gacagctggc
     3721 tgataccttc aatcagttag ctgaacgtat tgaagaagct caagatacaa tggaagctga
     3781 acgcaatcgt ttggatagcg tgctatccca tatgacagac ggcgtcattg cgacagatcg
     3841 caggggtaaa gtcattacga tcaatgatat ggctgtttcc ttgctagata tcaaaaacga
     3901 ggaagcaatc ggacaatcga ttttgacact tttagatatc gaagaagaat atacgttgcg
     3961 gaaattatta gaagatccaa acgaaatggt acttgaccgt tcaaccagta cattggaaag
     4021 tgatcagatg atcttgcgag tagacttttc tatgatccgt cgcgaatcag gttttatcag
     4081 cggattagta gctgtcttgc atgatgtgac agaacaagaa aaaacagaac gggaacgtcg
     4141 tgaattcgtc tctaatgttt cccatgaact tcgtacaccg ttgacaagta tgcgtagtta
     4201 tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa gtagcaccaa attttttgaa
     4261 ggtaacgcta gatgaaacag accgaatgat tcgaatgatc aatgacctct taaatctatc
     4321 tcgcatggat acaggtaata cccagcttca attagaatat gtcaatttca acgagttagt
     4381 gaattttgtt cttgaccggt ttgatatgat ggtgggaaat caggaaaaaa attataagat
     4441 ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta gatacagata aaatcatcca
     4501 agtagtcgat aatatcatga ataacgcgat caagtattca ccagatgggg gaacgattac
     4561 ttgtcgctta ttagaaacgc ataataacgt gattttaagt atcaccgatc aaggattggg
     4621 gattcctaaa aaagatctga accgagtgtt cgagcggttc tatcgagtag ataaagcacg
     4681 tgcaagagca caaggtggga caggacttgg attagctatt tctagagaag tgattaaggc
     4741 gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa gggtcgactt tttatatctc
     4801 gctgccatat gaaccttatg aggaggaatg gtgggaatga gaatctctga aaaagtcgtt
     4861 cgtataggac tcattttgat ggttgcgtta agcatatact tctcttatgc aatctggctg
     4921 agcccagcag gaaaaagttc aatcaacgta gatgagacaa acactcaaat gatcgagtct
     4981 caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc atgttacatg gcaccattcg
     5041 ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc gattgcaatt gatcatcgat
     5101 aaagcaagat ttggaaagct ttctgaagta gtttcaggag acaaatcaca gtttgaacag
     5161 gtgaaaaatc tgaactctgg ttttgagttg gcctataatg cgcctttttt gctaagagaa
     5221 tacaaggaag catttgattt agatttagat gtgtcgaact tggcaaaaga aagcgaaaat
     5281 atgtatttta ctagaataca attcgatcaa ggaaataaaa aaatccgatt tttgaattat
     5341 tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt ggacagatat caataaagaa
     5401 ttaacagcaa aagacacaaa atggacagaa atgacaacag aaccatcgac gatcgacacg
     5461 caatatgcta cgaaacattc gatcaaactg aagaagtata gttatatcct gtcacagcaa
     5521 ccctatacac tttttagaaa tgcttttttc cagaaaccag atagcgtgaa aaataatgat
     5581 gaaagcaatg aattgatttt ttacggtggg aatgaaacaa tgtctatcca ttcggaaacg
     5641 caagtagttg attttcgtgg agagctgcca gagaaaaaag aaaatgctag tgtattttct
     5701 gaaagtttcc cctatattag caaattaggc agttctttag ggaacctcag atattttgac
     5761 cgcagtgaca atacgatcga ttatcggata ttcgtggaag gcttcccggt ttttgggaca
     5821 gacagcagag gacagattgg tgtggaagtt ggcggtgaaa cgaatggttc tgtgcaagtg
     5881 aatatccaaa ccagtttgaa tacgattcaa gtgccgatac cttcagacga agaagtagaa
     5941 cttgcaagta cgaatgaaat catccagcag ttaaaagcta agggagcaga tagcaacaag
     6001 attggttcag tgatcgttgg ctatacatgg caaaatgtca aagaaacgaa tcgtgtcgtt
     6061 gatctcttac ctgaatggta tgtgaaatat aaagataact ggtattcagc taacgacctt
     6121 ctggaaaaat tacctggatt ggaggcgaat taattggatt tcaaaaaaat cgaatggata
     6181 tttttcgtcg cttttttagg gttgaatgtg tttttattca gcagttatca tgacgcggta
     6241 taccaagaaa acaatgtcat tcgctctaat cagactgaat caatccaaca acgtctggct
     6301 agcgacgata tcacttacac ggggaagatt tcgtctgaga acaagcaagg ctattatctt
     6361 agcgcggagc agaccaatct tagcacttca ttagcgaatt atcgcaaggc tggtaaaagc
     6421 ggcttgctgg caagtggtgt gtttttagac ggtgatgttt tgacgaaatc tttatccagt
     6481 tcagatgctg aaaagaacgt gatcgatcca gataaaatta gtagttcctt ggaagatttt
     6541 ttatctaaca aagacaatat tttattcggt aatgattatg tgtacacgaa aaacttttct
     6601 catttagaag aagaaccact agaaatccaa gcaagccaag agtatgaagg catcccattc
     6661 aatgatgata cagcaaaaat cgttctttct ttagaaaaaa acgataatga ttatagcatc
     6721 acgaaataca tgcagacgca tttatctgat atcgagcagc tgcgcgaaaa gacggaactg
     6781 tatacggaag aggaagcaat caatacattg tacatcaata acaaaatacc aagaggatca
     6841 aaaattttat ggcgtcagct agcttattcc tgtattctga aagtccggga aaaaaatgtg
     6901 tatgttcctg tttggcatgt agcgatcgaa acaagcgata aagtggtcca aattgagagc
     6961 gtcaacgctt tcagcaatac gatcgtgaca aacaatactg taccaaaagt ggaagatcaa
     7021 taaaaaagcg agtataatga aaaaagttta caaagtaaga agggaagtag gagtcaaatg
     7081 gctgaagaaa aaccagcttt taaaatcagt atcctagcaa gtggaagttc gggtaattca
     7141 ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg caggacttag cggtaaaaaa
     7201 atcacttcct tgatggctca aattgatcgg acaccggacc aattagatgg aatacttgtg
     7261 acgcatgaac atcgagatca tatacatggc gtgggtgtct tggctcgtaa gtatcatttg
     7321 gatgtttttg ccaatcagca gacttgggaa gcgatggaac cactgattgg aaatgtacca
     7381 gtggaacaaa aacatctatt tgagatgggg aaagtcagaa cattcggcga tatcgatatt
     7441 gagagtttcg gcgtctccca tgacgcagcg gctccgcaat tttatcgttt ccataaagaa
     7501 aatcggtcat tcgtcatgtt gacggacacc ggatattgca gtgattatat gcgaggtgtc
     7561 attgaaaatg ccgatggtta tttgatggaa agtaatcatg atcttgagat gttacgcatg
     7621 ggaccttatc cgtggaattt aaaacagcgt attttaggag atagaggaca tctttccaac
     7681 gaagacggtg cacttgtcat gactgatgtg atcggtaatc ggaccaaacg aatctatctt
     7741 ggccatctta gcaaggaaaa caatatgaaa gaacttgctc atttaacaat ggaaaatgtt
     7801 ttgaaagaaa aagattttgg cgttggacat gattttgaaa tctatgatac agatccagat
     7861 gaagcaactg agctattttc gatttaaaca gttcaatcaa taaaaatata aaaagcagaa
     7921 gataaaatca atcagggttg gttttatctt ctatttttat ttttttagca tattaaaaaa
     7981 ataaccgtat tatcgactga ctacactgat gtgctgcttt tcatgtttct catccagtat
     8041 ctcatctact aatgcaatcg cgtaatctgc catgctgatc gtactgtcgc tagcagcgtt
     8101 tctttgtaaa taatcatcac ttaacaaata agtacctgta cgtttgccat gtggatcaaa
     8161 taaagaagct ggactgatat aagtccaatg tagattttta gattgtttca attttaacaa
     8221 tccttcgtac atattgaaag ctgttggata gaagggtgca tttttaggta cttggtcaat
     8281 catacgagtc gtcttttctg tatctaagaa taacgaagag gcacccccaa cgacaattaa
     8341 gcgtgtatcg gttccttcaa gcatctcaat caaatgggac aaagaagttt gatgcatttc
     8401 ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca tcaaaagatg caagatcagt
     8461 agttgtcaac tcaaataaat ccttttgtat aaacggaaca tctgatggca atttttctgg
     8521 atgacgcacg attgccgtca cgtctaaatc tctggacaat gcttcgtcta aaataaatga
     8581 acctgcatgg ccagtagctc caatgagtgc aattttcata aaaaacctcc ttttttattc
     8641 ctgtccattg taagcagttg ctgaatattc gtcaaagaat ccgatttttg aaaagctcag
     8701 tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt aaattaagaa aactgattga
     8761 taaaaataga agttgaagga gataagaaat gaaagagtgg ttagatcgtg cctttttacc
     8821 aatgataaca tctgttgtga atcttagacc cttaactgct ttgcgtaatg gaatgactta
     8881 cacgatccca ataattattg ttggctcgct gtttttctta tttgctcatt ttcctgtgga
     8941 ttacatcgct cagtggatag acaaaacagg attttctatg tattttacac aagtatacca
     9001 ggctacattt gatgtgatag ccttttggat cgtttttgcc attgcatacg cttatgcaaa
     9061 agaagaagac atcgatcgct taccagtcgg cataacagcc ttgtctgctt atctactgct
     9121 tattcagcca ctaaatactc agatagatac tgtctggaca ggtgctgaag ggatactagg
     9181 agcagttaca actgggctca tcatcggatg gggctatgca cgtttagtaa gacttgcgaa
     9241 agggtggaag caaagagaga agatacctga aaatgtttgg cagtctttta cttcattgat
     9301 tccaatcatt gttatagtca cttttacgct tatcggttca gcggtttttc aacatttgtt
     9361 cagaatgact gttgttcaag gagtatggac atatgtccag cttcccattc aatcgttcat
     9421 agacacgttg tttggtgtgc ttttattagg tttttttgtt ccgtttttat ggttatttgg
     9481 tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc gtcttgcaag cgaattcatt
     9541 agcaaatgca gaaatcttag cctcaggaca agagttgacg gtggcaaatg gcggacatat
     9601 cgttactcag caatttctcg atcagttcat gacggttacg ggtgctggat tgacgttagg
     9661 ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa tacagagaat taggaaatct
     9721 tgctctttta ccagcctttt tcaacatcaa tgaatcaatt attttttcca caccgatcgt
     9781 gatgaacccg atgatggctg ttccttttat tttcgcaccg gtcgtttcag gattgattac
     9841 ctattccgct ttatattttg gattcgttcc tttatttaca gcagtccaag tcccttggac
     9901 aacaccgccg atcctctcgg gtttcttagt aggcggagtg cctgcagcga ttttacaagg
     9961 tatcgtgttg tcaattagtt ttttcattta tttcccattt ttgaaaaaaa tagattctgc
    10021 aaactgcaaa aaggaaagac agacaaaaaa tgatggagca gcagaaaaga tgaacgacta
    10081 aaaaacaaaa atactcctgt cgctttagat tgggaaaatc accaatctag gatgacagga
    10141 gtatttatag tcatgtttat actagccctt tttggacagc taattgaaca agaacaacta
    10201 atgtgttcat tcctacgtgt gtgatcatcg atgtccagat ccgaccagtt gctttgtact
    10261 ctaggctgaa gaagaaacct aggaaaaagt agattagcag atgaccatca ttatgagcaa
    10321 acgcaaacag caaagagctg atcactgctc cgacccaaaa atttgaatat cgactgatga
    10381 tccccagtat actgcggcgg aatacgaact cttccatgat gggtccagca acagttgtcg
    10441 ccaaaataaa agcgggtgct tccatgatca tctggatgat attttgtgta ttctcggaag
    10501 gggttgcttt gccaaacaat acagattcta acatgacagc gatgctttgc agaatcaatg
    10561 cgacaaagat tccaaccaat ccataaataa caatctttgg agcagaagct ttttttaccg
    10621 ctgattcgat cgctaagggt tcacgttgct taaaataaaa aatagctagg acaacggcgc
    10681 ctagtatata catgactgca gttgctgtga tcaattcaga cgtagatttt acaagtccta
    10741 tcgaagctag gatcaagggt gagataaaaa ttagaccata gcaaaaaatg ctgaggaagc
    10801 tatatttttt taaagacatg taattcctcc ttttaaggga taacaagcat gcaaggcaag
    10861 cttatatcga ttgatttcaa ttatatcata atttcaataa gcagctgtca gaataaaatt
    10921 tcttttcttt gatttaaaag cttgcaaaaa agagggagaa tgagtattat taaaaatgta
    10981 ggttagcact cgaaggttaa gagtgctaac agattaaaaa aagagttatc ggagggattt
    11041 atcgtgttaa aaccattagg tgatcgcgtc attatcgaag tcgcaaaaga agaagaaaaa
    11101 actgtcggag gaatcgtttt agcttctgct gctaaagaga aaccccaaac tgggacagtt
    11161 gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa aagttcctgc cgctgtaaaa
    11221 gcaggcgacc aagttatgtt cgaaaaatac gccggtacag aagtaaaata cgaaggaaaa
    11281 gaatatctga tcgtcgcagg aaaagatatc atggcgattg ttgagtaaat ttggaagata
    11341 aaaaaataat caaaaataat caatgaggtg aatcaatcat ggcaaaagaa ttgaaatttg
    11401 cagaagacgc acgcgcagct atgttacgcg gggtagataa attagcagat acagtgaaag
    11461 taacattagg acctaaaggc cgaaatgtcg tactagaaaa atcttacggc tctccattga
    11521 tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt agaagatcat tttgaaaaca
    11581 tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa tgatattgca ggagacggta
    11641 caacgacagc aactgttctg acacaagcaa tcgttcgcga aggtttaaaa aacgtaactg
    11701 caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt ggcaacaaaa gcagcagtcg
    11761 aagaattgca taatatctca actgttgttg attcaaaaga agccattgca caagtagcag
    11821 ctgtttcttc gggttctgat aaagttggac atttgattgc tgatgcaatg gaaaaagttg
    11881 gaaacgacgg tgtcatcaca atcgaagaat caaaagggat cgaaacagaa ctagatgttg
    11941 tagaaggtat gcagtttgat cgtggttact tatcacaata tatggtaaca gataacgaca
    12001 aaatggaagc tgttctagaa aacccatata tcttgatcac agacaaaaaa atctctaaca
    12061 tccaagatat tctgccattg ttagaacaaa tcttgcaaca atcacgtcca ttgttgatta
    12121 ttgctgacga tgtagatggt gaagcattgc caactcttgt attgaacaaa atccgtggaa
    12181 cattcaacgt agtagctgta aaagctcctg gtttcggcga tcgtcgtaaa gcaatgcttg
    12241 aagatatcgc tattttgaca ggtggtacag taattactga cgatctaggt ttagaattga
    12301 aagatgcaac aatcgaaaac ttaggaaatg cttctaaagt tgttgtagat aaagacaata
    12361 caacgatcgt agaaggttct ggagaaaaag aagcaatcga agctcgtgtg caattgatca
    12421 aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga aaaattacaa gaacgcttag
    12481 ctaaattagc aggtggtgta gctgttgtta aagttggtgc agctactgaa acagaattga
    12541 aagaattaaa attacgcatt gaagatgcat tgaatgctac acgtgccgca gtagaagaag
    12601 gaatggtctc aggtggtggt acagctctag tgaacgttat cagcaaagtt tctgctgtag
    12661 aagcagaagg agacgtggca acaggtatca agatcgttgt tcgtgcttta gaagaaccaa
    12721 ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt gattgttgat aaattgaaga
    12781 acgtggaact aggcactggt ttcaatgctg ctactggaga atgggtaaac atggttgaag
    12841 caggtatcgt agacccaact aaagtaacac gttctgcctt gcaaaatgcc gcttccgttt
    12901 ctgccttgtt attaacgaca gaagcagtcg ttgcagataa accagaacca gcagcgccag
    12961 cagctcctgc aatggatcca tcaatgatgg gcggtatgat gtaagaaaaa gcaacttgct
    13021 tatctacaac tgatcattag tgaaagacag ataaaaagag gctggaacag aagcgtttaa
    13081 ctccaagaaa taagaagaaa ttcacggaaa ttgcttttca aatttttgtg aatttcagct
    13141 tatttccgaa ggagttgctt ctgcttccgc tgtttatacg tgtttagagc gtgagacaaa
    13201 agtgttcttt acttttgtct cacgct
//
LOCUS       DAHHZJ010000067        13121 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.068, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000067 DAHHZJ010000000
VERSION     DAHHZJ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.068"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            45..212
                     /locus_tag="NBL02_001552"
     CDS             45..212
                     /locus_tag="NBL02_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HCD2572238.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(274..2085)
                     /locus_tag="NBL02_001553"
     CDS             complement(274..2085)
                     /locus_tag="NBL02_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCD2572239.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(2082..2447)
                     /locus_tag="NBL02_001554"
     CDS             complement(2082..2447)
                     /locus_tag="NBL02_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HCD2572240.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2610..3005)
                     /locus_tag="NBL02_001555"
     CDS             complement(2610..3005)
                     /locus_tag="NBL02_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCD2572241.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(3023..3985)
                     /locus_tag="NBL02_001556"
     CDS             complement(3023..3985)
                     /locus_tag="NBL02_001556"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HCD2572242.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(3985..4197)
                     /locus_tag="NBL02_001557"
     CDS             complement(3985..4197)
                     /locus_tag="NBL02_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HCD2572243.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4218..4916)
                     /locus_tag="NBL02_001558"
     CDS             complement(4218..4916)
                     /locus_tag="NBL02_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCD2572244.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(4936..5478)
                     /locus_tag="NBL02_001559"
     CDS             complement(4936..5478)
                     /locus_tag="NBL02_001559"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCD2572245.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5610..6614)
                     /locus_tag="NBL02_001560"
     CDS             complement(5610..6614)
                     /locus_tag="NBL02_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCD2572246.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6611..7600)
                     /locus_tag="NBL02_001561"
     CDS             complement(6611..7600)
                     /locus_tag="NBL02_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCD2572247.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7597..8403)
                     /locus_tag="NBL02_001562"
     CDS             complement(7597..8403)
                     /locus_tag="NBL02_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2572248.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8569..9525
                     /locus_tag="NBL02_001563"
     CDS             8569..9525
                     /locus_tag="NBL02_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCD2572249.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9602..10120)
                     /locus_tag="NBL02_001564"
     CDS             complement(9602..10120)
                     /locus_tag="NBL02_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCD2572250.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10208..10357)
                     /gene="rpmG"
                     /locus_tag="NBL02_001565"
     CDS             complement(10208..10357)
                     /gene="rpmG"
                     /locus_tag="NBL02_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCD2572251.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            10585..11031
                     /locus_tag="NBL02_001566"
     CDS             10585..11031
                     /locus_tag="NBL02_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HCD2572252.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(11226..13121)
                     /gene="asnB"
                     /locus_tag="NBL02_001567"
     CDS             complement(11226..13121)
                     /gene="asnB"
                     /locus_tag="NBL02_001567"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HCD2572253.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         4078 a         2789 c         2250 g         4004 t
ORIGIN      
        1 ttgttccata atagatataa caaataataa atgaggtgga ttcaatgaaa aaatttgtat
       61 ctggaatatt agtaggaagt ctggcaacag ccgctgccgt tgctggtttg gttgcttctg
      121 tgaaaaaaac agtgatcgat ccaattgacg aaaaagaagc aatgatcgaa gaaaaccgta
      181 aaaaagcaat gcgtaaacgc gtttctcgtt aataattatt taaaaatacg catgaagact
      241 ttctaaaaaa cagataagtc atcatgcgta tttttatttg tcagtcaata taagttgtgc
      301 tattttatct gcctgtctca gattccatgg atctgtataa ggtctagcta ctgctgcagt
      361 tagccaatcc acaccttcaa caggtattcc atagcaataa aagtcagact ggatttcccc
      421 tgaagcatca atcacgcgat tcgtacctcg ctcaacatct atagcttctg aatgataact
      481 tcctgtatta tctgaaaatg aaaaagggcg aacaagtttg ttttccttca aattttttat
      541 gacaggatta cttgaacgag aaaagcttgt ttgtggcaat cgagcttcaa tcaaatagcg
      601 actgctgatt tctttatctt gtagagaaat aacatagcgg ttgtgctttt gctgaatatc
      661 aaatggcggt tcgatcaaat gaaaaatacc cgcatctatc agtgctgcta attctttcgt
      721 ccgaatgatt ggcggaccga tcgttaaaaa gctatttaga tgagtaaacc atccccatag
      781 tatttctttg tattccttag cagaaaaaac gtgccattca atagcctgcc ggattggatc
      841 acgccagtct tttaatgcat caaatgttga agcaaatgct cctgtgcaat tgcctttttc
      901 cgcttctttt atctgccctt caataaatat tcggctagct tctgaaaagg gctcattctg
      961 ataggcaaga ggagtttcta aatacgacca atcccatcta taagggatta gtgctggatg
     1021 ctttccgaga cactcctctg aagagaggga gagaaaatca tgctcaaaat ttttctttgg
     1081 aatcggcaag tgatattctt cgatgatttt cttgtaataa aacaactcaa cgtctttttt
     1141 cagatagtca aaaaatgttt cgccagtcaa aaatcccgac tgatgccatt gctccaggtt
     1201 ttcttttgtt agtaaccgtg gccatgccat tgctccgcct tgcttttgat ttcttcctcg
     1261 agggtaataa gggagccccc ttcttgatcc actgtataca atcggttcct tgcctgatgg
     1321 atgatagacc agttttccct tttcgttctc gaagcgccct cctcttgata ctgtaaacaa
     1381 tccgacataa tcaaaaaaac ttaagcctag acctcttaaa aagacatttt ctttaggcgg
     1441 tatcttcgaa agatctacat ctgcaggatt agaaggaagc tgatacagta aaacattttt
     1501 ctttgcgtag tctacccaca ctctttcttc ttctgtctca tcattttccc agtgaccgct
     1561 tgctaaaaca agctgatcga tagaatagct ttgctgatct gtctgtaaaa gataaccatc
     1621 ttttcttttg cttacttttt ttactaaaga ataaacaaac tgaatagaag ctggaaattc
     1681 ttcttgcaac tttgagaaaa accagcgttg atagagtccg tataaacatc gagtagattg
     1741 ctgatctttt tccagacgat tgatttcttc tataattagt tgcttatttt tggttaccgt
     1801 ctgttcgata taatctgtag caggtccttt actccattga aacaagttgg gtccctgtgt
     1861 taccaatcct ccactggaca atgtttcatc tgtaaaaagc gtcacttgtt gagaaacaga
     1921 attcattaat aactccggtg gctgattcaa ccgccaaact cgacctccag gaccattggg
     1981 atcgaaaaga aaaagttcaa gagaatgtct gtcctgatta ttatggagaa tcctttctgc
     2041 tacacttaat cctctaggtc cggcaccaat gatccctatt ttcatttgca tgcctccatt
     2101 tcgataaatt ttttcaataa aacagcatgg ttctgatgtt cattttttgc accataaaca
     2161 aaagtaatga ctggatgttc tttcataagt tgactgagtt taaaaatagc ttgcttcttt
     2221 tcttcttcga aaatcaattc ttgctggtat ttctttgcaa atgaagcaaa tcgttctggt
     2281 acatggtcaa aagttttgcg tgcttcatta ctaggtgcaa tttctttcaa ccacagatct
     2341 aactgcgctt tttcttttga aattcctcgt ggccagaatc tatccacgag tattctatag
     2401 ccatcctctt tttcagcatc aaggtaaaca cgttttaatt tgatcatagt tgtttccttc
     2461 tttctacctt ttcgcttagc ttatatatgt agataatagc acaaaataaa aaaaatgaac
     2521 ggcacaacta tccattgcgc cgttcatgtg atcttggtca aaatctaatg attgattcgg
     2581 tttttaaagc tgtcatacaa tcatctgttt tatacttttt tacttttgat ttttccagac
     2641 catccgtcat atccaccctt aagtaaataa atctcttgat aacctgcttt acgtaattta
     2701 tttgctgtac ggatgctcaa acttttcttt tgatcgtaaa tatagacagg ctggtctttt
     2761 cgaatagatc ctaatgaatc tttcaaaaca gtgtaaggga tgtttcttgc acctaaaata
     2821 tgcccagcat caaacacatc tttttcgcga acatcgatta cttgtgcttt tctcattcct
     2881 tctcgaaatt cttcttctgt caaagtcgtt gctgaacgtt tggccattac tcgtagatag
     2941 acttcccaaa ggatgattgc aagcacaatc aatagcaaaa tgatattaat tacccataaa
     3001 accatgtaat gcctccgttt ttttaagcaa atttcaaggc taatgaggca gcaccaatga
     3061 caccagcttc attaccaagc tctgctaatt tgatttttgt tgattctctt acttgcggga
     3121 aagtaaattc tttaaaatat ttctctacac ggctacgtaa aaattctcct gctgccgata
     3181 ctcctccacc taacacgata cttgacggat ttaatgtatt ccccaagttt ccgcaagcta
     3241 aacctaagta gaagcatacg cgatcaacaa ccatcaatgc aaacggatcg tcttcttgag
     3301 ccatttcaaa gatatcttta cttgttactt cttctccgtt gtctagcatt gctttcagtt
     3361 ttgaatcccc tgcgtattct tctgccaaga aacgacctag gcgtaccaca cctgtagcgc
     3421 tagatacagt ttctaaacat ccgcgtttac cgcaagtaca ttcgaaccct tcaggatcca
     3481 cagtgatatg tccgatttct cctccagctc cgctcacgcc gtgaatcaaa tttccgccag
     3541 caacgatacc tccgccaaca cctgttccta gagtaacgaa tacaacgtct ggatcgtttt
     3601 ctccagcacc tttccatcgt tcacctaaag cagcaacatt cgcgtcatta tcgatagcga
     3661 atttgattcc tgttcctgcc tcgatcagtt ttttcacaaa ttgaacttct gtccagttta
     3721 agttatacgc accaataacc gttccagctt cgctgtccac gcttcctggg gtacccatgc
     3781 cgattccgat aaagtcttcg gctttcatat tatataaatt caaacgatga ttgattgatt
     3841 caatgatttc aggaacgata tgcttaccat catccaaaat attggtatcg atgctccatt
     3901 tttgctgaat ctctccttct ggtgttaaaa ttgcaaattt tgctgttgtt ccacctaaat
     3961 caatcccaat aatcttcttg tccattacaa atttcctttc tttcggcttt cttcaattcg
     4021 gtgctctctt tttaatatgc gccacgctgt catgtatgta tcatgatcaa tcaaacggct
     4081 atcatacaga ttacgcaatt cgatagtcat taattcaatg tcatagatgc gtgcacccac
     4141 atagataaag atgccaaatt ttttgaacag ctgctgtaca tcatataagc tctccatctt
     4201 taagcacttc cttcatatta tacaagtaaa ccatattttt ttaaacccag taaaatacat
     4261 attacaaaaa gaaatacaaa aactagtgta gaaagaatcc tttggtgtat cgaatagctt
     4321 gtctgttgtt tcggtagacc caaaacattc cctaacacta atccgccaat tagcccaccg
     4381 acatgtcccc aaacatctac agaggtatcc agaagaccaa aaacaaatgt caacacaata
     4441 aataaaagaa aatgtctgac tagctgctgg atcgctgggt tatgtttaaa atgaaaccct
     4501 aagataaaca acgcaccgaa caaaccgaat atcgaagtac ttgcaccacc agacagaaca
     4561 ttcgcttcgt taaaagcaaa gcttgctgta tttcccatta tcccgctgaa catataaatc
     4621 aagaaaaaac gccaatggcc atagattgcc tcgatttgct gccccataaa atacagtatc
     4681 agtgagttca ttacgaaatg catcagtcca aaatggatga agattggtgt cactaaacgc
     4741 caccattcat tgaagtgaac aagataagga ccatacattc cggcataata agggatctca
     4801 agtcgcggaa ataattccat catgaggaag accgctgtct gtatccctaa aaaagcatag
     4861 gtccataacg gtcgtcttaa ccatgcacgc aattttattt gctgttgata attcatgaaa
     4921 acctcccatt tcattttagt caataaacaa ttgttggatc ttctgatcaa agctctccgg
     4981 ttgccattgt tcattcaatt gttcacgaaa aacaaggcta catgtttttt tatgaaaatt
     5041 atttaaatac cggtcgtaat aaccaccgcc aaaaccgatt cgataaccgg ctctattgta
     5101 gataatacct ggtacaatga ggagatcgat catctccggc aaatagagct tctctgattg
     5161 tggctcaaga acaccaaaac tcgaacgtac aagaacggac tgttcatcca tttcataaaa
     5221 cgccatctga cgtttcggca aagttttagg tacaactatt ttcttttgtt ctttttctgc
     5281 tcgttggata accggctgtg tatccagttc aaattcattc gataaagtcg tccctatcac
     5341 ttttgctgac ttccagtact cgctggcgaa tagctgttta tagattcgct gttctttttc
     5401 ctttttcttg ccacttttgg ccagattttt cagcttttcg attcctttcc ggcgtaatct
     5461 tactttcaat tggtccaaaa actcaccctt ccttctctca taaaatggta taggaaaaaa
     5521 agaaaaatag caatcgaact ttcaaataaa tgcataaata gaataaaaag aagacctaga
     5581 aatgattggc cttccttttt taatgaattt caactgttca gcgcgtgttt ttgtctgata
     5641 aaaagtgcat acagaaaata tggcgcacca aaaagggcaa tcaaaattcc tgtaggtact
     5701 tccccactta gtagaatcac tctagcaacc atatctgcac taagcagaaa aatggaaccg
     5761 aacaaaccgc tcagaacaaa ggtccagtta ttttctttac gtacgactag cttcgcagca
     5821 tgaggagcta gtagtcctac aaaagataaa ctacctgtca ccgctacact accgcttgcc
     5881 aaagcaacag ctaacaacaa aaggattttt tgacttttag ccacgttaag gcctaatcct
     5941 tgcgcgcgtt cttgaccaaa tgatagcaat acaatcctct gtgcataaag tcctcctagc
     6001 ggaattaaga taaccgccca tggaagcaat gcccaaacat aaggccagct cgcaccccag
     6061 attgatcccg ctaaccatgc attcacaaat ccataatttt ccttagataa ttggatcgtg
     6121 catagcaatg ttaatgcaga aaggccggca ttaacggcaa taccggacaa taagattcga
     6181 ttcataccca aaaagccatt tcgatcatag gccgcaagat agacacagca agcagctaaa
     6241 acaccaccta gaaaggctac aactagcaat agaaacggca gcgaatagtg cgaaaagaaa
     6301 cctaaataaa gcatcactaa aaatcctgct ccggcattaa tgcccaatat actagaatct
     6361 gccaattcat ttcttgttac tccttgcaag atataaccag aaacgccaag acaagctcct
     6421 gctaataaac tgatcagtaa tcgaggaact cgaaattctc taacaatcag cgccaaagaa
     6481 gatgattctt tccctatgaa cgtggcaaaa agctgatcca ggtaaataaa aggcgtacca
     6541 atcattaaac tacctataga caggatgaca agtacaaaaa gcaagaaata gaacgtccaa
     6601 tatatttttt tcaccgttgt tccctcctta cttgtatcag taaaaacgga acgccaatca
     6661 atgcaatgac taatccaaaa ggtgtttcaa aagggggatt gatcatcctc gctgtaaaat
     6721 ccgccaacat aaccaatcca ccgccaacta aaaaagagac aggtagaaca taacgatagt
     6781 ccgttccaac cattccgcga acaactggcg gaaccaacag cccaataaat gaaacaggtc
     6841 cgactaacga aacggcaatg gctgctaaca ctgcaactag acagaaaaca atcaaccgcg
     6901 ttcgtttagg atgacttcca agagctaatg catgtgtttc actcaaatga atcaaagata
     6961 ttgcttttcc taatgaaaaa gaaaccaaaa cagcaagtat atataaagga aacaatactg
     7021 ctaattgttt ccatgtgata ttggcagctc cgcctacaaa ccaaaaagca agatcctgct
     7081 gcagattaaa gaattgggta agtgcttggc tgatagcagt aaaaaaagaa ctaatagcta
     7141 cgccagctaa aatcagctgt aaaggattca tgcccatttt cgaaaactgc aaaaaaccaa
     7201 atacgagcaa agttacagca aaagaacctg ccaatgacat tagaaacaca gaaaaatcag
     7261 aagctttagg ccaaaaagca aaactaaggg ccattgccaa cgaagcaccc gcgttgattc
     7321 ctagtaagcc tgaatctgct aaaggatttc ttgtcactcc ttgcatcaat gcaccgctca
     7381 atgcaaaaca actccctaca agaaatgctc ccgccacacg aggcaaacga atatttcgta
     7441 tgatttggtg actttgtttt gatctatcaa aatcaaacaa cgcctgccaa aaatctgctt
     7501 ttcccacagt cgctgcgcca tttgaaagag caacaaaaaa aataatcaaa agaacaaaac
     7561 agaccaaaag caggagtcta tttttcttca tagtcctcat aattgatcct cctgctcttt
     7621 tggcaacaaa tcataggtta gaaaaactgg tttatttgtt cttggaagcg tgacaatcgt
     7681 tgcatcaatc tcaaatattt ttttcataca ctcttttgtg atcacttctt caggtgtacc
     7741 ttctgtgatc aacttaccgt ttttcattgc taataaataa tctgaaaaac gtgaagcatg
     7801 attaatgtca tgaatcgaca tcaagatcgt tgtttgtttc atttgattga tttcagtcaa
     7861 taactgtagg atctctaatt ggtgtgccgg atctaaaaat gttgtcggtt catccaagat
     7921 caagatatct gtttcctgag caagcgccat agcgatccag acacgctggg tttgtccacc
     7981 agacagcgtt gcgatatccc gcatacgaaa tccagctaga cctgtagcat ccaacgccca
     8041 ctcaattgct tcatgatctt gcgtgtccat gccactgaac agtttttgat aagggtatcg
     8101 tccgtaagat actacttctt ctactgtcat acctgtaggg tgttcactag tttgtgctaa
     8161 taatgcaatt ttttttgcaa gttcttttga actgtatgta gagatatcag agttattgac
     8221 aatgatcttt cctgatttca caggtagtat acgggtaaaa cttttcaata aagtagattt
     8281 tccacttcca tttggcccaa tcaaactagt gattttccct tcaggaatcg taacagacat
     8341 gttttcaatg atttttttgt tttcgtagcc ggctgcaatg tcagctgctc ttaatctcgt
     8401 catttcaccc atcctttcgc tgaaaatcat tctcaattat gacgctgttt caacatttcg
     8461 tcaagcgcta ctttcatatc tatagccaaa tgaagcggtt gacagcgttc gaaatatcag
     8521 gaataataac caatgagaaa gattctcaat tatgttagga ggaaagaaat gaaattaaaa
     8581 acaaaagtat tcacattatc tagcatattg ctgctggctg gagtactgag cgcttgctcc
     8641 ccctcttcag ataaagaaac agctacaact gccaattcga cgacttcatc caatagtact
     8701 catacagtca cagatacact tggacataaa gtagagattc caaacgcgcc caaacggatc
     8761 atcggcagtt acttagaaga ctatctgatt gcactagggg aaaaacctat cgcccagtgg
     8821 actgttggca gtggttccat tcaagattat ctgcaagatg atttaaagga tgtcccaacg
     8881 atttcttatg accttcctta tgagaaagtg ttaagctttg agcctgattt gctgttgatt
     8941 tcttcttccg ctactgtcga aggcgggaaa tacgaacaat atagcaaaat cgcgccaact
     9001 tatgttgtaa aaaacggaac agatgtcaca tgggaagaac aactaaaaga tgtcggcaaa
     9061 gctttggcaa aagaaaaaga ggcagaaaag atcatttccg attatcaaaa atccatcaag
     9121 aaaactcgag aagaactagc agataaaatc gagaacaaga cagctgcagt gttgtgggtg
     9181 accaacaact cagcctttat ggtcagcaaa aatcggtcaa gtggaagaat cgtatacgac
     9241 gatctggaat ttggtgttcc tgatctagtg gaagaggtct ccaaaaaagc cacttctgac
     9301 tggtctgctg tttcctctga aaagctagct gaattagatg ctgactacat catccttgtg
     9361 aacagtgaca aagatgctgc catgttcaat gaaccaagct ggaagaatct taaagcagtg
     9421 acagacaatc atgtactgca attcggacct gaatctagtt ggctatataa cggacctatt
     9481 gccagtcagg aaatggtcaa tgatatcaaa gaaaaactaa attgatttta actaaaaaaa
     9541 gcatcttcac gattagtcgt gatatgcttt ttttcttatt aacaaactag acgcatagat
     9601 tttattcttc ttttgaagca accctatttt ttactctttc ccatttttga aaacgatggg
     9661 catatagatt ttcttgatcc gttatcccct catactcctc tataagggcg aagtctgaaa
     9721 aatcaatatt cccaatatac gtatcgcctt caaacgtgct atcgatcaat gttcgccaga
     9781 tccgaccagt gtaaggaaga agttcttgaa aaatccgact accacccgaa acatagatat
     9841 cttctgagat cgtttgacta tacgacaaaa gttcatcgat cgaatgcatt acttccgcat
     9901 tttctttcgt gcgaaaattt ggctgcgttg tcaacacgat gatatggcga taaggaagtg
     9961 aaagatcccc cattccttca taggtctttc ttcccatcac taaaattcga tccattgtat
    10021 gttcgcgaaa gaaacgcata tcatttggca atcgccatgg taacaaacca tttttgccga
    10081 tcagaccatt tttatcttgt gcccacatgg aaataaacat attatccgct ccttatgatt
    10141 ttagatgttt tgagataaaa aaagtctcaa ttctttgacc agcaaggaat tgagaacttt
    10201 aaaaacatta ttttgtttca cggtgcaaag taactttgcg ttcgcgtggg caatattttt
    10261 gtttttccaa acgatcagga ttgttacgtt tgtttttgct tgttagatag ttacgttctt
    10321 tacaagaagt acactctaaa gtgatgttta cgcgcatgtt tttccctccc atttttacaa
    10381 gaatcctcta agatgactct cagacttgat tatcttacca tgattttttc tagattgcta
    10441 gtgtttttca cgattttgtt aagtaaaact acttgacgat aaatacaacg gttttacttc
    10501 tcaaacaaaa tctttttctt tttgtataaa tacccttaca cttgtttata tagattatgt
    10561 tttttaataa agggggatac taatatgcca tggaacatga aggactttcc tgcatcaatg
    10621 aaaaacttag ataaattgac aagaaaaaaa gcaatcgaca tcgcaaatgc attattagat
    10681 gaagggtacc ctgataaccg tgcgatccca attgccattg atcaagcaaa aaaatggtat
    10741 gaagatgcta gtgaatcaga aaaacgaact ttcgaaaaag aaaaaaatcc aagcaagacg
    10801 gatgaacacg atacgaatcc tcgtgctgga aaactgcttg attcagatgt gatcgtagaa
    10861 tatgaggaag aacaatggat cgtgaaatct aaaggggcaa aaaaggcaag caaccatttt
    10921 gatacaaaaa aagaagcaat cgaaaaagga aagcaagtag cacaaaacaa agaatcaact
    10981 ttgttcattt ataaaaaaga tgggacaaaa gaaaaagaaa tcgattattg aacatatgta
    11041 aaggggttgt gacgaaaatc gtgtcacaac ccccttatgt tcgaaacaat ggtgttcctc
    11101 tgccaactcc gaaccataag tcataatatc tataaaacgg gaagagctgt tttttgaaat
    11161 tgtttcttat cactcagtgt taaacggtag gagaaaacct cttttttatt gatatttgat
    11221 tttcatcaaa cagtcacata gtcgattgct tctgcttgcg gaatcgtttc atctacaaag
    11281 tagactttat accatgtcaa gaaactgaag attgtccaga tttttctttg ctcttcttta
    11341 cgtccttcgt gatggtcatc tagcaacttc atgatttttt cttgatcaaa aaattctttt
    11401 gcaaaatctg cttcgaacaa tgctttgact tgttcgtagc cgtgttcttc tttcaaccaa
    11461 gctttgattg gcacagggaa tcctaatttc acacgattcg accatttttc cggaagatgt
    11521 ttgttcgcag cttgtctgaa aatatctttt gtgttgtttt gattcaataa aaacttactt
    11581 ggtatttgtt gagccaattt catgacttcg atatccaata aaggtactcg gacttccaaa
    11641 gaacttgcca tagaaagttt atctgccttt aacaagatat cttttggcat ccattgatga
    11701 agatcgacat attgcatctt attgacttca tctttgattc cttctgtttt ctcgtaatga
    11761 acagtcatga tttcatccac agatggtgct gtttgatatt ttggtgttaa cacttctgat
    11821 gcttctgatt cttcgaacac tcgagcatga ccgataaaat attcttttgc aggagcaagt
    11881 gattcatata aatggatccg tccatggaaa tttttcattt ttccgatttt acgtcctaaa
    11941 ttatagcgtg ttccttttgg taattttttc aatccttgag caatcacacg gatgaatttt
    12001 gaattcgtat ggaatccata cgtttggtat cccgcaaaca gttcatccgc cccttctcca
    12061 gattggacta cccgcacact ttgtgaagca agatttgcta aaaagtacaa aggtacgcaa
    12121 gaaggatttg aatcaggttc atctaagtga tactggatca aagggaatgc tttgaacgac
    12181 atgtctccgt cgatcactgc tgctgtatta tctaagtcca atagttcggt tagttttctg
    12241 gcttcaatcg cttcattata ggttttatca tcaaacccga ttgagaaaga atgatctggt
    12301 ttcaatacag aggttacata actggaatca accccgctgg ataagaaaga accaacttct
    12361 acatcactga ccgtatgtgc ttcaatagaa gcttgaacgg tttcgtcgat tttttcgatc
    12421 cattcttgtc gggaatgcgt ttcttttgtt tcaaaatctg catcccaata ttgctgaatg
    12481 tctatttttc catctttata cgtataataa tggccttcag gcaaacgata gacatctttg
    12541 aagaatgttt cgccgtttaa tggagaatat tggaaagtca tataaggttt caacgcttct
    12601 ttgttcaatt ctttgttaaa atcaggatga ggcagaaaac ttttgatttc tgaaccaaac
    12661 ataaatgttc cgttcatttc tgcataataa tatggtttga tcccaaagtg gtctcttgca
    12721 ccgaataact cattcttttc attatcccaa atagcaaaag caaacattcc gcggatcttt
    12781 tgcaataatt ctgttcccca ttcttcgtaa ccatgcaaaa ggacttctgt atctgcatgt
    12841 gtttgaaaaa cgtggccagc tgcaatcaat tcttctctca aaggctggta attatagatt
    12901 tcgccattaa aaataatgat ctttgtccga tcttcattgt aaataggctg agtaccgccc
    12961 tctaagtcaa taatacttaa tcttctgaat cctaaagcaa catgtttatc gatatattct
    13021 cctgaactat taggtccacg gtgaacgatt cgatccatca ttgtattaat aattgttttt
    13081 ttgttgtcct tgtcgttaac aaaacctaca ataccgcaca t
//
LOCUS       DAHHZJ010000068        13070 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.069, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000068 DAHHZJ010000000
VERSION     DAHHZJ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.069"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            35..1774
                     /gene="yfcC"
                     /locus_tag="NBL02_001568"
     CDS             35..1774
                     /gene="yfcC"
                     /locus_tag="NBL02_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCD2572254.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1931..3115
                     /gene="iadA"
                     /locus_tag="NBL02_001569"
     CDS             1931..3115
                     /gene="iadA"
                     /locus_tag="NBL02_001569"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCD2572255.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3226..3429
                     /locus_tag="NBL02_001570"
     CDS             3226..3429
                     /locus_tag="NBL02_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCD2572256.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3530..4192)
                     /locus_tag="NBL02_001571"
     CDS             complement(3530..4192)
                     /locus_tag="NBL02_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2572257.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4205..5707)
                     /locus_tag="NBL02_001572"
     CDS             complement(4205..5707)
                     /locus_tag="NBL02_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2572258.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVTVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6255..7934
                     /locus_tag="NBL02_001573"
     CDS             6255..7934
                     /locus_tag="NBL02_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCD2572259.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8061..8540
                     /locus_tag="NBL02_001574"
     CDS             8061..8540
                     /locus_tag="NBL02_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HCD2572260.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8759..9439
                     /locus_tag="NBL02_001575"
     CDS             8759..9439
                     /locus_tag="NBL02_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCD2572261.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9447..10778
                     /locus_tag="NBL02_001576"
     CDS             9447..10778
                     /locus_tag="NBL02_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCD2572262.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10793..11755
                     /locus_tag="NBL02_001577"
     CDS             10793..11755
                     /locus_tag="NBL02_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HCD2572263.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11866..12204
                     /locus_tag="NBL02_001578"
     CDS             11866..12204
                     /locus_tag="NBL02_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HCD2572264.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12283..12990
                     /locus_tag="NBL02_001579"
     CDS             12283..12990
                     /locus_tag="NBL02_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HCD2572265.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4153 a         2312 c         2876 g         3729 t
ORIGIN      
        1 caaaattact gaaaaaagaa aggaagaaat aaatttgaag aaaacaaatt cttcaggaaa
       61 accgaaacgc gacttcacta aattatcgac accccatacg tatgtcatta tttttggtgt
      121 agtcatcttt gcatggatac tgacttttgt cgttccagca ggaaagttca gcacgcaaga
      181 tattgaatac aaagatgcaa atggcgaaac aagtactcgt actgttttac gtcaagattc
      241 cttccgttat gcttacgagc tggataagtc gtatgtgttt gatcaattag aagaactgca
      301 agatcatcca gcagaacgtg aaaaactgga tgttccggaa aaaggattgg aaaaagtcat
      361 tgctgacgga gaaaaaaatc ttacgcagga aaaactagat gaaatctcct tgacagatga
      421 tgtgctttac gatcagtacg gcgaaaatat ctatgatact tctaaaaaat tgcataaaac
      481 agccaaaata tggggaacag atgattttgg cggctttggt ttcttaaatt ttgtttttga
      541 aggactagta tctggagata agtatggatc tgctgtaggg atcgctgcat tgatccttgt
      601 tgttggaggt gcttttggta ttattatgcg taccggagcg atcgacgcag gtatctatgc
      661 gtttatcagt aaaactaagg gactggagcg tttagcttta ccgctattat tctttgcttt
      721 ttcattcggt ggcgctacat tcgggatggc agaagaggta attccattct caatggtgat
      781 ggttccattt gtcattgcgt taggatatga ctcgatcgta gcagtaaccg tgacttatgt
      841 cgcatctcaa gtcggaaatg cgacctcttg gatgagcccg tttagcgtag cggtggctca
      901 agggattgct ggaatccccg tgttgtctgg tgcgacattc cgattgatta tgtgggtagt
      961 tgttactgca ttagccgctg gttatatgat gatatatgca gaaaaaatcc gtaaaaaacc
     1021 agaaagttcg ttgacttaca aatcagatga ttatttccgt tctcatatca aaaagacatc
     1081 tgatgaaaat aaaaaattca tgcttgggca taaattgatc ttattagaga tgttagttgt
     1141 ccttgtctgg atcgtttggg gcgtgactca aaaaggctat tacattccag aaatcgcttc
     1201 tcaattcttt gtgatgggat tagttgctgg aatcattgct gttcttttca aattagatgg
     1261 aatgaaagtc aatgacatcg cttcttcttt ccaatctggt gcagctgatt tagctggtac
     1321 agcaatcgta gtaggtatgg ccaaagggat tcttcttgtc ttgggaggat ctgatgcatc
     1381 tgttccttca gctttgaata cgattttgca tgggatcggg acggcgttga ccggtgttcc
     1441 agctgtcatt ggtgcttggg cgatgtacat cttccaaagt ttgttcaact tggttgttac
     1501 atcaaattca ggacaagcag ctttgactat gccaatcatg gctccactgg cagatttagt
     1561 aggtgtatcc agacaagtag cagttcttgc ttatcagctt ggttcaggtt ttatggacgc
     1621 ttttacaccc gtatcagcca gcttgattgg tgtgctaggt gttgcacgga tcgaatgggc
     1681 aaaatgggca agattccaaa tcaagatgca aggattcctg tttgttttag gaactatttt
     1741 catcatgatt gccattgcaa taggtctgca gtagtactgg agacctgtaa ataaaaaagt
     1801 aatccaaaca gtagataagg actccaagaa ccgtcatttt acggtaaaat caaaggagta
     1861 aagatcctat cgtttggatt attttttaag atcctatcat cagttatact taaagaaaga
     1921 ggaaaaatgt atgaaaatca ttcgacaaat tgaagtgttt gcaccagatt acttaggaaa
     1981 aatggatgta ctgattgccg gtgggaagat tttggctgta gaagaacagc tagaaggcgg
     2041 ctacgaagga gtggaagtag aagaactttc aggtgaggga aaagtactaa ccccgggttt
     2101 cattgactgt catttccata ttttaggtgg aggaggagaa ggcggttatc aaaatcgaac
     2161 gccagaagtc acattgagcc aattgactac agctggtgta acgacagttg tcggttgttt
     2221 gggaacagac ggagaaggta gagatatgac tgcgttgatc agcaaagcga aaggtctgga
     2281 agcagaagga atctccactt atgtatacga aggttcgtac cgattgccgg taaaaacagt
     2341 aacagattct atcatcaaag actttttgac aattgataaa attatcggaa tcggagaaat
     2401 cgcagtatcc gatcaccgtt cttctcagcc aagttttgaa gagttcgctc gtgcagtagc
     2461 agatgctcgt gtaggaggca tgttatccgg aaaagctggg atcatcaatg tccacttagg
     2521 cggaggaaaa agaaagctag agctattgac acgaatcgta gaagaaacag aaattccgat
     2581 cacacaattt ttgcctactc atgcaaatcg cacaccagaa ttatttgaag cttgcgtggc
     2641 atttgccaaa cgtggtggca cgattgactt cactgctagc gaagatcctg atttttggga
     2701 gaagacagat ggtgaagttc gcttcagcaa agcactgaaa cgattgatcg aggaagatgt
     2761 ttctttagat aatttcacga tgacctcaga tggacaagga agcttgcctt attttgatga
     2821 aaacaatcat ttcctagggt taggagtagg aagtgccaag gcattgctag tagggatcaa
     2881 agaagctgtg caaaaagaaa gtatcccttt ggaaatcgcc ttacgagcta taaccagtaa
     2941 tccagcacgt atattgaaat tggataaaaa aggaaaaatc gaaattggtg ctgatgcaga
     3001 tctgtgtatc ttggataaag aaacgcttga tattgataca gttatcgcta aaggagaaat
     3061 aatggtccaa gaaaaagaag tcaaagtatg ggggactttc gaaaaaagtt tttaatcaac
     3121 agctctaact atctttttta gaaaacgagc gttctgtttg tctgtatctg ttgttgatgt
     3181 taccctgaag gtagatcaat aaacaaacta agaggaggaa gcacaatggc agatttaaaa
     3241 ggacgttcta aagatatgaa agataaagta gtcggtgaag gaaaagaagc atacggcaaa
     3301 gcaacagacg acaagagcaa agaaacagaa gggaaagcac aatcactagg tgctgacatc
     3361 aaagaaaaag ctcgaaattt aggcgacgaa gtcaaagaca agttttctcg aaaagacgaa
     3421 aaaaaataaa aaccacttat tactaattga atcaagcaaa taaaaaatcc gaaccatatc
     3481 tgcgagacag atatggttcg gattttttat ttgatcatta tcctgttttt taaacttcga
     3541 ttttttgaaa ttttttattt tttagttcaa agacttcatc acagcttttt gctacttcaa
     3601 tctcatgagt cacgatgata atacagcgat tttgttcatg ggcgatttct tggaaaagtt
     3661 tgacgatatc ttgtgaagtt gtttcatcta gatttccagt aggctcatcc gctacgatta
     3721 gttgatgttc acagcagata gcacgaacga tagcgactcg ctgctgctga cctcccgaaa
     3781 gtttggtcac ctgttttttt gctaatattt catcaattcc tacacgagcc agtcgatcta
     3841 aagcaacttg tcgataattc tcttgcttgg tcttagtgat ttccattgct gctatgacgt
     3901 tgtctagagc cgataaataa ggaagcaagt tgtaagcttg gaaaataatc gagacatcat
     3961 ttttacgata acttcttaac ccgattttgt tcagtgaacg atcttgatag tatacctttc
     4021 cttcttttgg tgcatctagt ccagaaataa gcgaaagaaa agtcgttttt ccagaaccac
     4081 ttgctcctaa aatagcgtac atttttccag cttcgaaatt cagttctaca tctttataaa
     4141 gcgcatcttg cggatcttga taccaaaatc ccaattgttc tgtccgtaac atcaaattcc
     4201 tcctttagtt taataggatt tttcttgggt ttaatcttaa tatccctata gaagctaaaa
     4261 agacagaaaa cagactgatt ccaaatgcta ctccggctaa tagtcccaat tgtgcaggtt
     4321 tgacattgat ttccaattcg ctgacttgat cagaaacagc aaatggatta ttcgtggttc
     4381 cttgttgacc atcgcgttga ccgccttgtt cttgtctgtt acctggcatt tgtccaccgc
     4441 caggagcttg ctggttttgt gtcgtggttt ctgtctgttg gtcaagtagc tgctgtccca
     4501 cggcattcgc gacgacgttt ccgctaagcg aagcaactcc taatgcaaga atcatacaaa
     4561 tagccacttc ggtaaacaat tggagaatga ttttacttct ggattctcct aaagatagca
     4621 agacgcctaa ctcatgccga cgctcccgaa tggtaatcat aatgatcaga gtaagtatga
     4681 taataccggc tacagtcact agtaaaacaa catttttaga aaaacttgcc acgttgttta
     4741 aaggttccaa cattgactga tacatggaat catttgattg taagctaaat gtatctgtgt
     4801 cgatcagctt ctcagcttct ttgagaaact catccatttt atctggatca gatagagtgt
     4861 aaatagcaga atctatcgtg ttatcgtcag atgtcccatt tagttcattt gcaaaagtgt
     4921 aagaagtgta aagtgtattc gacggattca tgaagttgaa tttcataccc atactgccgc
     4981 ttgtttcgct ggattcataa attcctttga tcgtcatttc ataagtggta tcttcatctt
     5041 cagtactcgt gattgtgaaa gtatctccga ctgccaggtc atttgtttct gctagagtag
     5101 aatcaatgac tacattgttt gtgcctttgt cgctgtcatc tattccttca ccatccgtga
     5161 tttttgctgt tccatcagaa aaattactat tttgtgcagt tgctgacaca ccactgattt
     5221 gaaagtctgc ttgagtcatt tgcggattcc cgcccattcc tccagcaggt tcaccgcctg
     5281 catcagtagt ttctgaactg tcttcacttg aatcagaact agtgatggca gttattccat
     5341 cttttgccat agctgaggcg gaactttcaa aggaataact ctttacatta tctaattcag
     5401 ctattttttc cgcgtccgtt aaggaaactg gtgtaagaga aaaactgcct ggatctggac
     5461 gttcttcagt ggtagaaccg cttgaagaag tttgttcctg ttttttaaat gaagcttctc
     5521 gattggcact taaagtcact gttgctccaa cttctttttg tgcttgattc gctgcttctt
     5581 ctgcagcgct tcgaatcgtg aggccagcta gcacaaaaat aagaatggct gtgaacacgg
     5641 caaacaataa caatgaacgt ccccatttta cttttgtact tttccatgca cgtttcataa
     5701 aattcatatt ttgcatccct cctagtagat gcatttgata atagacaacc tatcttaaag
     5761 ctagcttaaa tcttatgctg tttgcacacc atcaacatga ggttctgtat atataaaaag
     5821 tcactatagt tattgcaaaa aatgtggtaa aatgaaaaaa gtatgtttca aaccgacgaa
     5881 gaagaaatga aagaggaata gcaaaagaaa tcctagatgc tacagaggga tttttacgtg
     5941 catagttgtt ctaacaattg ctgatactag cgatttagtt tatttgttgt ataataatgg
     6001 ggaacagctg aatacttata taagtattct ggctatagcg tttcgcaaaa gatccatttt
     6061 gttaatgaag tatatccaaa ataaaatgcc catccaactg tcagaacgcg gatgtgctgg
     6121 taattttcct gctgattctc cataagttga gaaatagtga ttttgcatat gcttcattgg
     6181 aatggctcat atatttagat aaagaagaaa atcgacatct ttaaacttgg atgtcataag
     6241 aagaggtgaa gtttgtgagt accattaaaa ttatcccatt aggcggcgtt cgtgaaaatg
     6301 gaaaaaacat gtatgtcgca gaagtgggcg aggatatttt tattttagat tgtgggctaa
     6361 aatatcctga aaatgagttg ttggggatcg acgtcgtaat ccctgatttt acgtatttag
     6421 aagaaaacat tgatcgtgta gctggtgtct ttttaacaca tggacatgca gatgcgatcg
     6481 gggcacttcc ttacttgttg tcaaaaattt ctgtaccagt attcgggaca gagttgacga
     6541 tcgagttagc aaaattgaat gtcagtcgta atgaagcagc caaaggattt aaggatttcc
     6601 atgtggtaga tgagcataca gaaatagact ttggtgaaac agtagtgagc tttttccgta
     6661 ccactcatac gataccagat tcagtgggga tcaatctgaa aacaaaagaa ggtagtatcg
     6721 tttatacggg agattttaaa tttgaccaaa cggctatccc aatgtatcaa acagattatg
     6781 cacgattggc ggaaatcggc aaagaaggag tcttagcctt actaagtgaa tcggcaaatg
     6841 cagaaaatcc tcagcctgta gcttctgaag cccaaatagc agatgaagta tttgatacga
     6901 tcaaatattg ggaagggcgt atcatcgtcg cttgtgtagc aagtaatttg caacgagtgc
     6961 aacaagtatt ggatgcagca gaaaaagcac aacgaaaagt tgtcttgact ggtcaagatt
     7021 tcgaacgaat tattcgcaca gcgatgcgtt tagaaaaact tcaattacca agtgaagatc
     7081 tgcttgttac gttgaaagac atgaaaaact atgatccaga agaactgatc attttagaaa
     7141 ctggccgaat gggtgaaccc atcaagtctc tgcaaaaaat ggcaaacggt acacaccgta
     7201 atgtcaagat tgaagaagga gatttggttt atatcactac tacgccaagc atcgcgatgg
     7261 aaaccatcgt ggcgaaaacg gaagatatca tttatcgagc tggtggaaca gtgaagttga
     7321 tctctgagaa tatgcgcgtt tctggccatg ccaatcctaa tgacttgcaa ttaatgatca
     7381 atttgatgaa accaacatat tttgtgccag ttcaaggaga gtatcgtgaa ttagctgctc
     7441 atgcagattt ggctcatgca gtagggatgc catacaaaaa tatctatatt actggacgcg
     7501 gagacgtgct ggaatataag aacaagcgta tgagtgttgc aggaactaca agtgcagaaa
     7561 atgtaatgat tgatggatta ggcataggtg atatcggaaa cattgtttta cgtgatcgaa
     7621 aaatcttgtc agaagacggg attttcgtag cggttgtcac tatcagtcgg cgagaaaaga
     7681 aaatcatttc aaaacctcaa attacttcac ggggattcgt ttacgtaaaa gctagccgtg
     7741 atctgatgaa agaaagcagc aatatgattg aaacaatcgt tgagaaacat ttagaaagca
     7801 atgattttga gtggagcaaa ttaaagcaag acatccgaga tcaattaggc cgttacttat
     7861 ttgaacaaac aaaacgccgc ccagtgatct tgccagtcat tatggaagct actcaacgtc
     7921 gaaataaaaa ataaacaaaa agagtaagat gaacctctct atagagtgct cgtcttgctc
     7981 tttttttgcg aaaaacaaat aatttttgaa tacagcatcc ctcgctttat aataaacgat
     8041 agaataaagg gagtgaatca atgatgagtc aaacaaaagt cgaacgttat ttagaagaac
     8101 aaaagatcag ctatcaacca cttgatttta gtcatgtggt agaagaatct ttcacagaag
     8161 aactacaaaa aagagggatt gatccagctt tgatatgtaa aactctcgta gtgaaaggaa
     8221 acaaaactgg ggtgatcatc gcattgattc ctttgaacga tcacttagat tacaaaaaaa
     8281 cgagacaagt gacgaaagat cgtaaagttg gttttcctgg aatggatttt gtattaatgc
     8341 atacaggata tccccacgga gcaaacaccc ctattggaat aaaactcgca catcctgatt
     8401 attgtttttt agcagacagt tctttaaaag aaaaagaaga agtaattgtg tcaagcggtg
     8461 aaatcgggag aagccttcgg ataaaagtag ccgatttaga aaagatcgtc catcctattt
     8521 acgctgattt aattagttaa gggacgagaa gagaagctaa gcagagcaga gacctcaaac
     8581 ttgagattat atctgcttgg tacttggtgc atcctgtatt atctgttaca attgctttgt
     8641 acatactgta ctatcaatcc aatgaaaata tatttagcag ctatattttt tcattgatgt
     8701 gcaagtgatg aaatcgtttt taataaaaaa gatacagaga aaacgcgagg tgtaaaatat
     8761 gaaaattacg tatcatggac attcctgttt aacaattgaa atggaaaatg gccaacgcgt
     8821 gatgattgac ccgtttatta caggaaatcc attgacagac ttaactgcag aagaagtaaa
     8881 gacagattgg ttgctgatta ctcatggtca tagtgaccac ataggagata tgattcctat
     8941 tgcaaaagaa aatcaagcca caattatcag tattgttgag attgctgatt atgcgaacag
     9001 cagaggagta gattcattcg gtatgaatat cggtggaaaa catgcgtttc cattcggcgt
     9061 ggtaaaattc gtacatgcac agcatagttc aagctatgaa gtagacggta tcgttcagta
     9121 tatgggcgag cctagcggaa ttattattca agcagaagga aaaacgattt atcatgcagg
     9181 ggatactgct tatttcagtg atctaggatt gcttgcagaa gaattcgata ttgatgtcgc
     9241 tttcttgccg attggcgata actatacgat ggggccagaa gatgcagcaa aggctgcgca
     9301 aacaattcgt gccaagaaag tagttccaat ccattacaat actttccctg tcattgaaca
     9361 agatcccgaa tcttttgtag agttactgga agatagtgaa ggaaaaataa tgagcgtctc
     9421 ggaaacgctg gaagtgtagg cagaaaatgg cgacaaaaca tgatttgatt ttagaatata
     9481 ttgaaagttt acctgttggc aatcgaattt ctgtaagaag catcgcaaaa gaattgaatg
     9541 tcagtgaagg aacagcgtac cgtgcaatca aagatgcaga aaatgtcgga ctagtttcaa
     9601 caattcaacg agtagggacc atccgtatcg aacgcaaatt aaaaaaacat attgaacgtt
     9661 tgacatttgg cgaggtcgtc aggatcattg aaggggacgt tttaggcggg gcggcaggat
     9721 tagacaaggt cttgaataaa ttcgtcatcg gcgcgatgac taaacatgcc atggaacgct
     9781 atattacgcc aggatcacta atgatcgtcg gcaaccgggc ggaagtccaa aaattagcat
     9841 tggaagatgg cgccgcagta ttgatcacag gaggattcaa tacgactgct gagattgcaa
     9901 agctagctga tgatcttgaa atgccgatct tacgcacctc ttatgataca tttacagtcg
     9961 caaccatgat caaccgagcg ttaagcgacc aattgatcaa aaaagatatc atgcttgtca
    10021 gtgatatcta tatgccagct gaaaagactc attatttgca gcagacagat acgatttctg
    10081 attatcagcg tttgtcagaa gaaacgcagc attcgcgttt tccagtcgtc aaccgccatc
    10141 atcgtttgat ggggattgtt acggcaaaag atgttttagg gaaatcgcca aaccagatca
    10201 tcgatcgggt gatgacgaaa gaaccaatca gtgtaaagaa aacaatgagt attgcctctg
    10261 ttagccatca aatgatctgg gatgggctag aagtcatgcc ggtcgtttcc gatgacttga
    10321 cattggaagg cttgattacc cggcaagatg taatgaaagc gatgcagctt gtccagcgac
    10381 aaccgcagat tgcagatacg atttcggatc agatatctgg aaatatccat acgattgata
    10441 ctgaccgtga aggaaatcgt ttggaaaacc ctaagttcaa attcgtagta gcaccacaaa
    10501 tggtcaatgg cgttggaacc atttcttttg gcgtacttag cgaaatcatt tctgatgtcg
    10561 cacaaaaaac aatggtgatg aaccaaaaac gaaatatctt gattgaacaa gtgaacctac
    10621 attatttgcg gctgatccaa ttggaaagcg aattagacat tcgtccacgt gtattggaaa
    10681 tcggcagacg ttccgctaaa ctggatatcg aagtgtttat tgaaaacacc atcgtttcca
    10741 aagcaatcgt tgtttgtcaa gtaatggaac gttcttagaa agcaggagtg aaatgacagt
    10801 acagcaagaa atagtagata caataaaaaa atacgataaa atcattatcc acaggcattt
    10861 gcgccctgat cctgatgcat taggatcaca agtaggatta gccgaactat tacgggaaag
    10921 tttcccagaa aaagaaatac gtcaagtagg tgaaacgatt gaagggcttc gctttttagc
    10981 agaaatgcaa gaagttgaag atgtcttcta ccaaggagcg ctagtcatcg tgactgatac
    11041 agctaattca cccagaatca gtgatcaaag atatcagttg gcagaaaaac tgatcaaaat
    11101 cgaccatcat cccaatgatg acccttatgg agatcttgtg tgggtaaata caaaagcaag
    11161 tagctgtagt gagatgattg cagaatttgc tttgatgttt cctgaatctt taaagatgaa
    11221 cgttaaggct gcacggttac tttatgcagg aatcgtcgga gatacagggc gctttttgta
    11281 tccctccacg acgtcccgta cattagagat tgcttcgata ttgagaaatt atccatttga
    11341 tgcaagtgct ttgaacagag agattgaaca aatgccgatg aaagtcgcta aactttccgg
    11401 ttatctttat caaaatatcc aggtggatga aaatggtgca ggaagaataa tccttccgca
    11461 agaaattctt gataaatatg gaatcgatga ttcagagaca gcttcgatcg tttcacttcc
    11521 tggagtgatt gacgaagtgc tggcttgggg gatttttgtt cagcagcctt cagggtatta
    11581 ccgcgtacgt ttacgttcta aggggccgat catcaatgaa ttggctaaac gccatcatgg
    11641 aggaggccat ccattagcaa gtggtgcgaa tgcaaaagat ttagcagaag tagaggaaat
    11701 ctataaagag atccaagata tatgccaatc gtataagagc agaaaaacag aatgatactt
    11761 cgttcgaaaa cgtgcctttt gtagtaaaaa tcacaaatgt atcttttcaa gatcttcttt
    11821 ttccgttaca atagtgctca ctaaagattt taaaggagaa agaagatgac attaccaaat
    11881 tgcccagaat gtggctcaga gtacacatat gaagatcgag gcttgttgat ttgcccagaa
    11941 tgcgggaacg aatggaacaa agaagaacaa gaaactgtca tagaagacgg attagtggta
    12001 ttagatgcaa atggaaaccg tctgttagag ggcgatagcg tgacagtgat taaagacttg
    12061 aaagtaaaag gtgcgtcagg tgccattaaa caaggaacaa aagtcaagaa catccgttta
    12121 gtagaaggcg atcataatat cgattgcaag gtagaaggat ttggaccgat gaaactgaaa
    12181 tcggaatttg tgaaaaagaa ttaaagaata ttcaaggtct tttcacattc ttttcctact
    12241 actaatgata aaataaagta aacgaaaagg agtgaaacga aaatgtatgg ttatggaatg
    12301 tatggttttt tagatccgac atttcttttg gtcattcttg gattagttat ttccggaatt
    12361 gcgtcagcgt acgtcaatag tactttccga aaatacgatc aagtgagaag caaaaacaat
    12421 gtgactggta cacaagcagc acaatatata ttacaaagtc aacagataaa taatgtcggt
    12481 gttcaacaaa ttgccggaga tctcacagat aactacaatt caggtaataa gatgttgagt
    12541 ttgtctcaag caactgcaca gtctacttca gttgctgcga taggtgtagc agctcatgaa
    12601 tgcggacatg cggtacaaga tgcttcgggt tatgtcccat tgaagattcg tgcagcactt
    12661 gttccagcag ctaactttgg ttcgatgatt tcgttcccct tgattatggt aggtgtattg
    12721 cttagctgga actctacgtt aatcaatatc gggattttcg ctttttcttt agcattactt
    12781 ttccaattgg tgacattacc agtcgaattt aacgcgtctc ggcgagcaat ccaaattttg
    12841 agtgaaggcg gactactgac ggaagaagaa gtaccgatgg caagacacgt attatttgca
    12901 gcagcactga cttatgttgc agcagcgctt tcgacattcc tgcagctgtt gcgcttgatc
    12961 ttattgttcg gcggcaaccg aagagattaa aataaagaag ttgtgaaatc agcgctttga
    13021 tctgagctaa taccgagagt tagctgattg aataccattt atttggaata
//
LOCUS       DAHHZJ010000069        12926 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.070, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000069 DAHHZJ010000000
VERSION     DAHHZJ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.070"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..406
                     /locus_tag="NBL02_001580"
     CDS             <1..406
                     /locus_tag="NBL02_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HCD2572266.1"
                     /translation="EDALVIKKEQGFTGTETKSLFLKDKKDNNYVYLTFTTKQTDFKA
                     LGKIVGKRLSIVSSERMEEQTGQKQGAVSPFGYEKQIPIIIDEELLSNEKLVFAPGRP
                     DQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN"
     gene            443..1453
                     /locus_tag="NBL02_001581"
     CDS             443..1453
                     /locus_tag="NBL02_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HCD2572267.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(1568..2281)
                     /locus_tag="NBL02_001582"
     CDS             complement(1568..2281)
                     /locus_tag="NBL02_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572268.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(2471..3061)
                     /locus_tag="NBL02_001583"
     CDS             complement(2471..3061)
                     /locus_tag="NBL02_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HCD2572269.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            3545..3772
                     /locus_tag="NBL02_001584"
     CDS             3545..3772
                     /locus_tag="NBL02_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCD2572270.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            3788..4954
                     /locus_tag="NBL02_001585"
     CDS             3788..4954
                     /locus_tag="NBL02_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCD2572271.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            5188..6231
                     /gene="hrcA"
                     /locus_tag="NBL02_001586"
     CDS             5188..6231
                     /gene="hrcA"
                     /locus_tag="NBL02_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HCD2572272.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            6325..6888
                     /gene="grpE"
                     /locus_tag="NBL02_001587"
     CDS             6325..6888
                     /gene="grpE"
                     /locus_tag="NBL02_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCD2572273.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            6939..8768
                     /gene="dnaK"
                     /locus_tag="NBL02_001588"
     CDS             6939..8768
                     /gene="dnaK"
                     /locus_tag="NBL02_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCD2572274.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            8919..10085
                     /gene="dnaJ"
                     /locus_tag="NBL02_001589"
     CDS             8919..10085
                     /gene="dnaJ"
                     /locus_tag="NBL02_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCD2572275.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(10167..10832)
                     /locus_tag="NBL02_001590"
     CDS             complement(10167..10832)
                     /locus_tag="NBL02_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD2572276.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            10808..10981
                     /locus_tag="NBL02_001591"
     CDS             10808..10981
                     /locus_tag="NBL02_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572277.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(11209..11415)
                     /locus_tag="NBL02_001592"
     CDS             complement(11209..11415)
                     /locus_tag="NBL02_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD2572278.1"
                     /translation="MICKASSGDEMAVKEILKFYDGYISKLCLRPFYHSESGKIIMQV
                     DEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(11441..11860)
                     /locus_tag="NBL02_001593"
     CDS             complement(11441..11860)
                     /locus_tag="NBL02_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCD2572279.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            12364..12717
                     /locus_tag="NBL02_001594"
     CDS             12364..12717
                     /locus_tag="NBL02_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD2572280.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            complement(12777..>12926)
                     /locus_tag="NBL02_001595"
     CDS             complement(12777..>12926)
                     /locus_tag="NBL02_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HCD2572281.1"
                     /translation="NKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTVITEPDA
                     KTQSR"
BASE COUNT         4337 a         2229 c         2650 g         3710 t
ORIGIN      
        1 tgaagatgct ttggtaataa agaaagaaca aggatttaca ggtacagaga ccaaaagctt
       61 attcttaaaa gataaaaaag ataataacta cgtatatctg acttttacta cgaaacaaac
      121 ggatttcaaa gcattaggga agatcgtcgg taaaaggctt tctattgttt catcagaacg
      181 catggaagaa caaacgggac aaaagcaggg cgctgtttct ccgtttggtt atgaaaaaca
      241 gattccgatt attattgatg aagagctctt atcaaatgaa aaacttgttt ttgcaccagg
      301 tagaccagat caaacgatgg tcatccaaac ggttgactta aacaaaatca tccagctact
      361 tgagatgccg atatttttga tgccggcaaa cgaaaaaaac aattaaccaa gtaaaatagt
      421 gcatacttgg aggtgaagca acatgataag ttatccatta ttagaaagaa atgcaacgat
      481 tggggttact gcaccatcct caggcgtatc agaggaacac cattcgttac tcgaatcagc
      541 tttaaagaga atggaagaaa aatctttcca agtagttact ggaaagacgc tttggacgca
      601 agataaggaa aaatcaggtc cagcaagaaa acgagcagat gaattcaatc aaatgatgca
      661 agatgaaaat atccaaatga tctttccgac ttggggagga gaattattga ttgagattct
      721 cgattttgtc gattttgatg ctctacaacc aaaatggata gtaggttatt cagatattag
      781 tacgttgcta cttgctgtca cattaaaaac agggatagcc actgcacatg gaactaacct
      841 tattgattta agaggagagt acttggatga aacgacagca atgtgggaaa gtgtactgaa
      901 aacaagagaa ggagaatcga ttctacagca atcttctgaa aagtatcaaa aagtgtggcc
      961 ccatgagcat tcatcatctt ctgttttcca tctcactgaa ccaacaaatt ggcaatcagt
     1021 cagcggagat gcagtaagga tgaagggacg attattaggt ggttgtattg atacgatcag
     1081 gcatttaatc ggtactcctt atggagatgt ccaatctttt aaaagaaagt ataatgatgg
     1141 aaagccgatc ttgtggtttc tagaaaactg tgagctatcg tcagttgatt taagaagatc
     1201 acttgttcag atgaaactgg caggctggtt tgatggcagc tcggggatca tgttcggtag
     1261 aagtgcggca gaggatttgt tagattataa aatgaaggat gtatacgaag atctgtcaaa
     1321 ggaattaaaa ctaccgatct tgtatgatat tgattgtggt catgtaccgc cacaactgac
     1381 attgattaat ggtgcctatg cagaagtagc ggtagaagat agaaaagcga ctgttttgca
     1441 aacgtttatt taaaaaattt tttcctgcct tggagtctat caaagaccaa taaatctaaa
     1501 tagaaaagaa agtgaaagaa gctgacaaaa tgctgtcagc ttctttttgt ataacatatt
     1561 agaaagtcta attatctata actgtcacat cgattaactg tttcacgtca ttgggatttc
     1621 ctgtaggaat agctactaca gatgcattat caatgatttc ttgagttgtt tctctaatga
     1681 acgctggata agatgtttga tctgcaggca tttgccaatg tgaaggaact ggataactag
     1741 tgattgtccc gtcatggtta cttgaataca ctaaaactcc ctggtaactg tatttcccag
     1801 tgagcataac cacatctttc ggatagactg cactccctgc atcataaggg gcgataggtg
     1861 ttcctgcagg ataatgaata acatgtaatt cgaaatcacc cgatgtatct ctaaaagtat
     1921 tacctctaat agctagccaa acacgtgcgt actcaatttg tagatcagaa tatcctgcta
     1981 acggatctat tgactgagaa gactgtgagg aggttgtgtc tattgttgaa gaagaaatat
     2041 ttgtctcaac tgtactacta gatgtagaag aagtattggg ctctgagact ttactgcttg
     2101 ttgaagatgt attcgtttca gtagaagctg atttctgagt acttgatttt tcagctgaac
     2161 tggtttgtct gattgaactt ttgtctgtgg tcgcagatgg atcactattt tctttactag
     2221 gagacgtaca tcctacaagg aataaagaga ccaacaataa aataaaacct aactttttca
     2281 tataaaatac ctcattattt ttttaatacc agtatagcat aatcctgaat ttttgaatcc
     2341 gtaaaacctt gtctatgaat ataaatagag tgtatcttct ttgataaatg tcagaaaaag
     2401 tgtgacaaat aatcatcagt aaaattactt gtcacactat tgataaggag tatttaattg
     2461 aagagaatga ttaggagaaa ggatctccgt actttttata gtaatcatag aatccaagtt
     2521 tgtatagacg aatcgttttt tctcttattt cttctgtttc aaaatggact gcttgaagaa
     2581 tcaagtctgt gaattctata ggagctgttc cattcgcagt aatcaaatta cgatctttca
     2641 cagcttgttg ttctataaaa tcttctttat tctgataatt ttccgattct aaccaaggaa
     2701 attgcgcatt tccggtgtgt ttgtaacctt gcaataatcc gctctgagct aaataatcaa
     2761 ctgccccaca aatagcagct actggtacgt ctgtttttaa caatttatcg attatttgat
     2821 ataaatgatg attttttatg tcccatgaat tgccaccgat cataataaaa aggtcaactt
     2881 tttcaggtat ttgttcgatt aagtaatcga tttttgtttt taatcctcca atggaagtga
     2941 cctctgattg gatagaagca gttttgtttt gccatttctc attttggttt agtgtgctag
     3001 taatataagc accttcccaa tcggcatatt cctctaatag aaacgtaatc gcagttttca
     3061 ttttcaagct ctcctttact atctataaca aggacaacag ttttaaccta cagacattca
     3121 ttaaaattgg gtagcgacag cgaaaacaac aattcttaag ttttagacag agtatacaga
     3181 tagtcaactt aattaaatac gaactaaata aaaatgacag gtaatcattt ttgtttacca
     3241 gcattataac attttgcata catggatgac aaaagaacca attcccactt gcttgagtac
     3301 aatattaaaa gaaactggaa ggcaaaaagt aattatagaa acgaaaaata aatcatatcg
     3361 ggcactcaga catgaaaaca accctagaga tatataacta ggtaaccagt ttaatgaaag
     3421 aaaaaatcat ccaagaagtc gattcttgga ttttttagtg gatttgttcg ccaaatacgt
     3481 ttagtatgcg tatatgagag gttgtaagca aagcgttgtg gtctgagcac ttaaggagga
     3541 atctgtgaaa atagcttttc atatttttgc agattttgac ttaatgccga aggtcagctt
     3601 tgcgattacc gtttatcgag aggctgtaaa agcaccgtgt agctccgatg acaataagca
     3661 agcattttta gagctaggtt tccttgattc taaatatgcg gcttatttca atgagttggt
     3721 gcttgaacgc gattttaaga aggtcgaaaa caggcacgac ttttcgacgt aaggagaaag
     3781 aatttcaatg atagaaacaa taaatcccgc agaaaacaag gtttctgcgt acattcatat
     3841 cccattttgc gagcatatct gctattactg tgattttaat aaagtttttt tagaagggca
     3901 gccagtagat gagtatgtgg aaatgctgct gaatgaaatg aaaataatgc tagaaagtca
     3961 tccgacagcg aatttagaaa cactttacgt agggggcggg acaccaactt ctttatctgc
     4021 taaacaactt gatcgactgt tatcaggtgc tagagagatc ctgccttttc aaaatggtaa
     4081 tgagtttact gtagaagcga atccaggtga tttgacccgt gaaaaacttc aagtaatgaa
     4141 aaactatggc gtcaatcgtt tatccatggg agtgcagaca tttgataatc gcttattgaa
     4201 aaaaattgga cgtaaacaca ccgcagaaga tgtgtatcaa actatgcaat ttttagaagc
     4261 agaaaatttt accaatgtaa gcatcgattt gatttatgca ttaccgggac aaacactaga
     4321 aggatatctg gatactttgg aaaaagcttt ggctttggat ctgcctcact attctttgta
     4381 ctcattgatc ctcgaaaaca aaacaatgtt catgaactgg gtacgacaag gaagactcga
     4441 actgcctgat caggaaacgg aaaccagaat gtttgaagag acgattgaag caatggaaaa
     4501 gcatggacgt caccaatatg aaataagtaa ctttgggctc tccggacatg aatctcaaca
     4561 taacctcatg tactggaaca atgaccacta ttttgggttt ggagctggtg ctagcggtta
     4621 tcttggagat attcgttacc gcaacaaagg accaatccaa cactatttac gcccattacg
     4681 agcaaatgaa cgcccggtat tggaagaaga aaaattgaca cgaaaaaatc aaatagaaga
     4741 agaaatgttc ttaggactaa gaaaaaaaac agggattact aaaaaacatt tctatgatcg
     4801 ctatcaaaaa acaattgaat cggtgtacgg tgatgtgcta agggaattag agcaagaagg
     4861 gttacttgtt aatgaagaag accgtgttta tttgacacca agagggacct ttttggggaa
     4921 caatgtgttt gagcgctttt tgtttgaaaa atagtttaaa gaatatttgt tttttatctg
     4981 aatttaaata aaaaaagttc aaaaatacaa taaaggacta ttgaaacgtt gatataactg
     5041 gatttacatc aattatttag aattagcact cttgtgttta gagtgctaaa aaatggagtc
     5101 tttctcttga caggagaagg gcttcttgct atatttataa gtggattagc acttagaata
     5161 tatgagtgct aatgagaggt gaggaagatg ttaacaaagc gacaaaacga cattttacgt
     5221 ctgatcatcc aacactatac gagtagtggc gtgcccgttg gttccaaaac attgatgtct
     5281 gagggagtca aggccagctc agcaacgatt cgcaatgata tgaaagcatt agaagatgaa
     5341 ggattgctgt tgaagactca ctcttcttct gggcggattc catctgcttt aggctatcgt
     5401 tattacgtgg atcatttgtt aaaacctgct cgcgtagcag aagatgattt gctgctgatt
     5461 cgtcagtctc taggcagaga atttcatgaa atcaatgaga ttattaagca atctgctgag
     5521 atattatctg agctgacaag ctatacgact ttttcattgg gacctgaaat aaaagaccgc
     5581 cgactgactg gctttcgtat cgtacccttg aataatcgac agattattgc tatcgtcgta
     5641 acagatatgg ggaatgtaga aagccaagtg ttcactcttc ccgaatatat gggaagtcag
     5701 gaactagaaa aaatggtaca gattgtaaac gaccgtctag tcggagatcc tctgatgacg
     5761 gtgtaccata aacttcgtac ggagattccg atgattcttc ataaatattt ccaaacgaca
     5821 gaagggatgt ctcagttatt tgacaccgtt cttggtcatg tttttgaaga taaggtttat
     5881 gtcagtggac aaatgaatct cttggattat gagccccgtc aagatcttga tcagttcaaa
     5941 tcgatgtttt cttttatgaa agactcagac ggacttacac aaatgatcac tccgatggat
     6001 agcaatattc atatcaagat tggtgctgaa ttaggcaatg atttattgca gaatatgagt
     6061 atgattcaag caagttacga gatagatggt catggaagag gtacaatagc cttgttaggg
     6121 cctactagta tgccttattc tcggatgttc ggtctagtcg atgtttatcg aagagaacta
     6181 gcagctaaac ttgctgatta ctaccgttcg cttgacttgt caaactcgta atgtgaataa
     6241 cgaagctatt tgcagctgat cgttctctct ttcttagtaa gagcaatcaa acagctgact
     6301 gaataatgaa aggaagactg tatcgtgaaa gaaaatcaag aagaactaga taaagaaatg
     6361 acagatgctc agccagaacc ggaaatcgat gtcgaagctg cagaagattc gggtatttca
     6421 gaagctgaag cggaagaatt cgaaacagcg aaattaaaag ctgagttaga agaaatggaa
     6481 gataaatact tgcgggctcg tgctgaaatc gcaaatatgg caaatcgagg aaaaaatgag
     6541 cgtgagcaat tacaaaaata ccgttcacaa gatttagcca aaaaattact tccatcaatt
     6601 gacaacctgg aacgtgcatt agccacagaa gtgtcagatg atcaaggggc aggtttgaaa
     6661 aaaggcgtgg aaatggtctt ggaaagtttg cgtaacgcac tggaagaaga aggaattgaa
     6721 aaaatccctg ctaaaggaga agcctttgat cctaatcttc atcaagcagt acaaactgtt
     6781 ccagcgactg aagatacacc agccgatacg attgtagaag tactacaaga aggctacaaa
     6841 ttacatgatc gcgtgttaag accaaccatg gtcattgttg cacaataatt gtgcggatat
     6901 aaaacttaca ctttcaatta aagaaggaga cgttaattat gagtaaaatt atcggtattg
     6961 acttaggtac gactaactct gcagtagcag tattggaagg aaacgaagca aaaatcatcg
     7021 ctaacccaga aggaaaccgt acaacacctt ctgttgtatc attcaaaaat ggagaaatcc
     7081 aagtagggga agttgcaaaa cgtcaagctg tgacaaaccc aaatacgatt tcatcaatca
     7141 aacgccatat cggtgaagca ggttacaaag tagatgtaga aggtaaatca tatacaccac
     7201 aagaaatctc agcaatgatc cttcaatata tcaaaggatt cgcagaagac tatttaggtg
     7261 aaaaagtcga aaaagcagtt atcactgtgc cagcttactt taatgatgca caacgtcaag
     7321 caacaaaaga tgctggtaag atcgctggtc tagaagtaga acgtatcgtc aatgaaccaa
     7381 ctgcagctgc tcttgcctat ggtttagata aaacagatcg cgacgaaaaa atcttagtat
     7441 tcgaccttgg tggcggtact tttgacgtat ctatccttga actaggagac ggtgtctttg
     7501 atgtattgtc aacagctggc gacaaccatc taggtgggga cgactttgat aacaaaatca
     7561 tcgactacat ggtagcagaa ttcaaaaaag aaaacagcat tgacttgtct caagacaaaa
     7621 tggctttaca acgcttgaaa gacgctgctg aaaaagcgaa aaaagattta tctggcgtat
     7681 caagcacaca aatcagcttg ccatttatca cagcaggaga atctggacca ttgcatttgg
     7741 aaatgacatt gacacgtgca aaatttgatg aattaacagc tgacttagta gaacgtacaa
     7801 aagtaccagt acgtcaagcg ctaaaagatg caggtttatc acaatcagaa atcgacgaag
     7861 ttattttagt cggtggatct actcgtatcc ctgcagttgt agaagcagta cgtaaagaaa
     7921 caggaaaaga accaaacaaa tcagttaacc ctgatgaagt agttgctatg ggtgctgcta
     7981 tccaaggtgg cgtgatcact ggtgatgtga aagatgttgt tttacttgac gttacaccat
     8041 tgtcattagg tatcgaaaca atgggtggag tgttcactaa attgattgac cgcaatacaa
     8101 cgattccaac aagtaaatca caagtattct caactgcggc agataatcaa ccagcagtag
     8161 acatccatgt attacaaggt gaacgcccaa tggctgctga caacaaaact ctaggaagat
     8221 tccaattgac agatattcca gcagctcctc gtggtattcc acaaatcgaa gtaacattcg
     8281 atattgataa aaacgggatt gtaaatgtat ctgcgaaaga tctaggtaca caaaaagaac
     8341 aaaaaatcac gatcaaatct tcttcaggtc taacagacga agaaatcgaa cgtatggtaa
     8401 aagatgcaga agcaaatgca gaagcagata aagctcgtaa agaggaagtt gacctacgta
     8461 atgatgtaga tgcattgcta ttctctgtgg acaaaacatt gaaagaatta gaaggcaaag
     8521 tggacgaaga agaagtgaaa aaagcagaaa cagctcgcga tgaattgaaa gccgctgttg
     8581 aagcaaacaa catcgaagaa atgaaaacta aacgcgatgc attgaacgaa atcgttcaaa
     8641 acttgactgt taaactatac gaacaagcgg cacaacaaca agctcaagaa aacccagaag
     8701 cagctcaagg cggagcagat gatgtcgttg atgctgattt tgaagaagtc gatggcgacg
     8761 ataaataatc agttcactga atttcgagag aaggccaaag ttgctacttt ggctttttct
     8821 tttgaaaaat agtttgaagt atcggttttt gtttcaaaca aaatatgata tgataacaag
     8881 cgacgtgaaa atgatgagaa ccaataggag gaaagcctat ggcaacaaaa cgtgattatt
     8941 atgaagtctt agggctctca aaaggagcat cagaagatga aataaagaaa gcttatcgta
     9001 agctttcgaa aaaataccat cctgacatca ataaagaacc tgatgcggaa gaaaaattca
     9061 aagaagtatc agaagcatat gagattttaa gtgacccgca aaaacgcgct gcatatgacc
     9121 aatacgggca tgcaggcaca gatccaaatt acggtgctgg tggagctggt ggcggatttg
     9181 gtggattcgg cggcttctct ggtggcggct tcggtggttt tgaagatatc ttcgactctt
     9241 tctttggagg aggcggtcgg tcagtcgatc caaatgcgcc tcgtcaaggt gctgatttgc
     9301 aatatacggt cgacttgtct tttgaagaag cgatttttgg tgtagaaaaa gaaatcaagt
     9361 ataatcggga agaaatctgt catacttgtg gtgggaacgg tgcaaaacca ggcacacaac
     9421 caactacttg tcataaatgt catggtgcag gtacgattaa tgtagaacgc caaacaccac
     9481 ttggacgagt gatgagccgt caaacttgtg atgtttgtca tgggacaggg aaagaaatca
     9541 aagaaccttg tccaacctgt catggctcag gacatgagaa gaaagcacat tcagttaaag
     9601 tgaatgtacc agctggtgta gaagaaggac aacaaatgcg tttagctggt caaggagaag
     9661 ctggagaaaa cggcggacca tttggtgatt tgtatgtggt cttccgtgta gaagaaagtg
     9721 atatctttga tcgtgaaggt tctgaaattt actatgaatt gcctttaagt tttgtacaag
     9781 cagctttggg ggatgaagtc caagtaccaa ctgtccatgg agatgtgaaa ttgaagatcc
     9841 ctgctggaac acagacaggt acgaacttcc gtttacgtgg aaaaggcgct ccgaagttac
     9901 gtggtggtgc aacaggcgac caacatgtca aagtaaaact gatcacaccg aaaaatctaa
     9961 atgaacaaca aagagaagca ttacgtgcat tcgctgaagc tggcggcatc aaagttgaag
    10021 aacagcaaga agacggtttc ttcgataagt ttaaagatgc ttttggcggg aaaaagcgga
    10081 agtagttttt ctagtaaaaa cgctaagtca ttaataatta aaaaccagac gaacaatcat
    10141 atgttcgcct ggtttttttg tagttgtcat gtttgtacca acaaaaaagc cagctttaga
    10201 atatgataat cgtcatagtt tctaggggta taaccagtga gagaatggaa cttgttcaat
    10261 cgatattgca aggtgttttt atgaataaat aattcttctg agcaattttt gatgcttccg
    10321 ttatttttac tgaagcatgc caaagtcttc ttgaacaaat gtacttcttc ttctgtcagg
    10381 cttttcaaaa tacgttttac atataatgtt cgttgatctg acgggattgc tggtaaaacg
    10441 atcccgaggt ctaaatcttc aaaaaagcgg atctcgttca cagattgatg gaaagctatc
    10501 cattcgcaaa cttgtttggc atgaaggaaa gattgcggtg tctcttgctc tgtataacaa
    10561 agcgacccaa ttccaaagtt tagctgtaac tgatacattt tattaaactt tgtctggatt
    10621 ttatcaagca atgatagcaa tgaggctctg gaaatccgat tgactaggat aatgatttgt
    10681 tgtctatgta cagtgagcaa agcctgtccg taataagaga agaccctttc gagcaactcc
    10741 aataaaggat tgatccgttc ttgacacaat tgctgattgt cagaaagttg tcccacaact
    10801 acataatatg gagaagaaaa atcaagctca attaagtgag ggtggtatcg actatgtact
    10861 tgatatcatc aatgaaccga tggtactttc ttatgcgatg gaacaggtag agcgattgct
    10921 tgtttgtgca ggaaaacaag cgatgcggct gatacgatta ggtgagaaaa taagaagata
    10981 aaaatacacg gtcccttcat ggcgaccgtg catttttggt ttttattaaa ttttatatct
    11041 aattttcaat tatccgcata aacactatac attcaagcat tttgaagtat agaaaaacac
    11101 tcatcttacc acgaatttac cacgattgaa tgagccttga tatgaccgta aaactactaa
    11161 gggagataac acgaaactgt gtatctcccg atttttttat acagttgatt acttcactcc
    11221 gatttcaaat ttcaatattg ctttcatcat gtctgtatgt atttctcctt ttagttcttc
    11281 atcaacctgc ataatgattt taccactttc agagtggtaa aaaggacgca agcataattt
    11341 agaaatataa ccgtcataaa acttcaaaat ctctttaact gccatttcat ctccgcttga
    11401 cgctttgcaa atcaattcaa aagagggata gccataatct ttacttcctt tcgctcaaaa
    11461 gcatttttaa cttccttagt cctctagttc ttctactgca aatagcagaa cgtgatacat
    11521 ggtacagttc tgaaatttct ctgtcgttca ttccttgaaa ataacgtaat aaaatcgcac
    11581 tacgctgttt ttctgatagt ctatgtaaaa ctgtaccaag tgtttcatca gatacataaa
    11641 ttgtaaagtg caagacttta aatgatacac aatcataaaa acaggtatca atcgttccac
    11701 gttcaacttg tttcgtttcg gctatttcac aaaacaatac ttcacgttta gaatgtcttg
    11761 caagctgtct gtttcgactt ttcactttgt tcttaatgac aatcagcatt aaagaattga
    11821 actgtaatct gacgatatgc tcgaaagaag atgtcctcat aatctcaccc cctttctgtc
    11881 taggaaagga agtgagcctt gctcgtttat ctcctttcac tcttagtccc aatgtgaaag
    11941 ggggatttgt tgcattactg ataaataaac tttgtaaaaa agttctgaat agccaaaaaa
    12001 gcatataaac agatttattt ctctgtttac atgcttctgt tattctatct atatgattta
    12061 taaaaccaca ttggtggacg tacttatcta ttgcagatag acgacttttt ttgacaagaa
    12121 cccaatgtaa ggaaatttat tgtatatgat gtacttcatg gcgacgttga cctccaacaa
    12181 accgccattt ggaagtaata tacaatattt taacagcgta aatagcacta ccatataacg
    12241 gtttttttta ttggcgttta gtagtgcttt ttattaaata taaacctata aaccatataa
    12301 cacgtttttc tatacctgtt tttaattcag taggaacaat aaaatgtata gaggtggtct
    12361 actatgcgta aaaaagaaga taaatatgat tttagagcct ttggtttagc cattaaagaa
    12421 gctcgattga aacgaggttt aactcgtgaa caagtgggag cattgattga aattgaccca
    12481 cggtacttaa ctaatattga aaataaaggg caacacccca gcatacaagt tctttatgac
    12541 cttgtatcgt tacttcatgt ttccgttgat gaatttttct tacctgctaa taacttggta
    12601 aaaagcaccc gacgattaca gatagagaaa tacatggata gctttacaga caaagaacta
    12661 tccttaatgg aatctttagc cagcggtatc aacgaagcaa gaaacatcga agactaatta
    12721 aaagaatcca tacataacgg aaagagccga taaaatgaga ttgtattaat ctcattttat
    12781 cggctctgcg tctttgcgtc tggctctgta atcacagtta ctttgaactg ctttatttca
    12841 attaaatttt cttgtctgca tttcggacaa tagaggggga atttttttaa ttcagtatct
    12901 tcccttatct ttaatcgtgt tttatt
//
LOCUS       DAHHZJ010000070        12877 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.071, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000070 DAHHZJ010000000
VERSION     DAHHZJ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.071"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..435
                     /locus_tag="NBL02_001596"
     CDS             <1..435
                     /locus_tag="NBL02_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HCD2572282.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="NBL02_001597"
     tRNA            complement(759..842)
                     /locus_tag="NBL02_001597"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="NBL02_001598"
     CDS             1370..1870
                     /locus_tag="NBL02_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD2572283.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="NBL02_001599"
     CDS             complement(2060..2404)
                     /locus_tag="NBL02_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HCD2572284.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2558..3478)
                     /locus_tag="NBL02_001600"
     CDS             complement(2558..3478)
                     /locus_tag="NBL02_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCD2572285.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3665..4156
                     /locus_tag="NBL02_001601"
     CDS             3665..4156
                     /locus_tag="NBL02_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HCD2572286.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(4180..6555)
                     /locus_tag="NBL02_001602"
     CDS             complement(4180..6555)
                     /locus_tag="NBL02_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCD2572287.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6881..7507
                     /locus_tag="NBL02_001603"
     CDS             6881..7507
                     /locus_tag="NBL02_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCD2572288.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7530..8234
                     /locus_tag="NBL02_001604"
     CDS             7530..8234
                     /locus_tag="NBL02_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCD2572289.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            8426..9259
                     /locus_tag="NBL02_001605"
     CDS             8426..9259
                     /locus_tag="NBL02_001605"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCD2572290.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            9456..11384
                     /locus_tag="NBL02_001606"
     CDS             9456..11384
                     /locus_tag="NBL02_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HCD2572291.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            11522..11977
                     /locus_tag="NBL02_001607"
     CDS             11522..11977
                     /locus_tag="NBL02_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCD2572292.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            12447..12716
                     /locus_tag="NBL02_001608"
     CDS             12447..12716
                     /locus_tag="NBL02_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572293.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
BASE COUNT         3943 a         2428 c         2339 g         4167 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tagctgcttg
     2461 taaatgagaa tttagaagta agattctcgt ttaaatttat aaaaatgttc ttattagatg
     2521 taatagatta aatttattct aaaataaacc tgattcctca ttttttttgt gaacgatttt
     2581 ttatttctgg caatacgcca gttgttcgat acattttttg ttcgttttca tttgcaaacc
     2641 ggccaaatac tgaatgagtt ggtaaaatat tgataaatgc atcgtcgtcg atttcatgaa
     2701 cgatctgatc aagatcatac atttcgtagc gggtgatgac catcatgatc atgttccctt
     2761 gtttttttga atagccaccg acagacggca gtaatgtcat tccacgtacc atccgttttg
     2821 ctagttcttc tgttacctct tctggcttga cggtaacgat cattgctgtt actttttgat
     2881 gactcgtgtg gatggcatca attacttgag tcattgcata aatggaaata atcgtataca
     2941 aggcactttc ccagttaaag aagaagcctg cgatgactac aatgatacca tttagaatca
     3001 gcatgtagtt tccgactgtt tttccagttg tttgtgaaag tacaagagag atgatatcca
     3061 tcccaccagt tgtaaagccc atttttaaag ataatcctac gccaagacct aataaaacac
     3121 cgcctactag ggcattcatc aaaatattat cagtgatctg tccgactgga agcagcatcg
     3181 tcatcaccga tacacataca acattcacaa aactaaggat cgttgctcgt tttcctactt
     3241 tgagaaaccc taatacaaac acagggatat tcaaaagaaa aataaatgtt cctgtgtcta
     3301 tcttgatatg aaaccattca tataaaagcg aggaaatgat ttgagcgatc ccattcatcc
     3361 ctgcagaaaa gacattggct ggaatcaaaa aataatttaa agcgaccgca gcggtcaatc
     3421 cggttatcaa aataaccact attttcttta atctttcatt tttatgatac gcagtcataa
     3481 ttttactcac aagcacctct cccctacttt ggtattccaa atgtaagaat aacagtttat
     3541 ctattaaaat aaaatacttc gaactcaaat gagaagaatt ttagcaaaca ggtaagaatc
     3601 tgccctaatc ctatgataag atagatgtat ggtaaatatt tatatgtata tcaggagggt
     3661 tcctatggca aaagatgcta gcttcgacat tgtatcagaa gtcaatacag aagaagtaaa
     3721 aaatgcgatt cagcaaacaa caaaagaatt gtccaatcgc tttgacttca aggattctac
     3781 agtagaaatc aaattagaga acagcaatct agttgtattg agcgatgatg atttcaaaat
     3841 cgaacagatc aaagatgtat tatttggtaa gctgatcaaa agaaatgtgc caatcaaaaa
     3901 tatccatttt tccgaatcag aacacgctct aggtggcaaa gctcgacaaa ctgccgattt
     3961 ggtcaacgga atcgatcgtg aaaacgcgaa gaaaatcact acagctatca aaaatgaaaa
     4021 actgaaagta aaagcacaaa tccaagaaga tcagattaga gtgacaggta agaaccgtga
     4081 tgatctacaa gctgtgattg ctttattacg taaacttgac ttaccaatcg agttgcaatt
     4141 cactaactat cgttaatcaa aaaacgaggc tgacgtctgt cattcctcgt ttttttgatt
     4201 tactgcttct tttgataacc aaaaacgctg tattccttct tccaattccc caacacttcg
     4261 tacttctctt gctgtttgcc aatgttgtgg gaataaacgt cgataagaaa aactagaaaa
     4321 tcgttgatct gccaatacaa cgattccttg atccgccatg ccacgaatca ctcgaccacc
     4381 agcttgcaag actttgttca ttccaggaag ctgataagcg taagcaaatc caaggtgttc
     4441 cttctcatca taatatgatt tgatcagttc ctgctcatga ttcatctgcg gcaaacctac
     4501 acctacgatc atactgccga tcaatcggtc ccctctcaaa tcgattcctt ctgaaaaaat
     4561 cccgccaagt acgcaaaatc caatcaatgt ttcagcggga tttgtttgaa attttgttaa
     4621 aaatcgttcg cgttcttcct cattcatgtc cgtttcttgg attagcacat tgctgtcggg
     4681 atattgctgt ctgaatgctt ccaccgcaag atctaaatat tgataagaag gaaagaaaac
     4741 gaaataattc ccgacctttc catcagcaaa agcatgaatc gtctctacta attttgccag
     4801 actgtgttcc ctttttcgat aagtcgtctc taggtaattt gcgatgaaaa cttgctggtt
     4861 tccttctggg aaaggactcg gcagtttgta agccagactt ttttgtcccc ccagtgtttc
     4921 ctgataataa gagagtggtg aaaaactagc agaaaaaaga atactgcttt ttcctttatc
     4981 caacgactgc tcaagaaaca agcttggatc aatacaaaac tcttttacta tcagatcgcg
     5041 atacgttttt tcgactgttg tttcgtagtg atcatcataa aattcactga ccttcaaaaa
     5101 atgaagcaaa tcaaaataat aaggaagcaa ttgttcttgt gatgggtgct caggaaattc
     5161 tgccagccat tctttaattt tttcgcttaa ttgataacct gcctttacta aagattcagc
     5221 cggtgctttt tgatgatgat aatcccaatg atcttctttt gctatttctc ggatcgaatc
     5281 aaattctttt aatagtttgt tgaaacggcg atgaagtttt ttgaattcct ttggtatagc
     5341 ttccttactt cgtttcgttt tttcataaga tagttctgcc gaatacatct ctcgggaacg
     5401 attcaccaaa ttatgtgctt cgtcaatcaa aaacagatag tcctcgtttt tttcatcttc
     5461 aaaaaaacgt cttaaataaa cagttggatc aaataaataa ttgtaatccc caatgattac
     5521 gtcacaccag atactgacat ctaatgatag ttcgaaagga caaagtgtat gctttcttgc
     5581 ataagtttcg atgacttcac gagtgatctg attctcatga ttcaaaagat cccataaacc
     5641 ttcattaatc cgattgtaat aaccattggc atatgggcac tgatctggat tacaggtcgt
     5701 ctcatctaaa aagcagatct tgtctttcgc cgtcaaagtg acacttttta cttcagctcc
     5761 tgtatctttt aatgctgtca tagcatcttc agctacttgt ctagttattg ttttagccgt
     5821 tagataaaag atcttttctc cctcttcttc ccctacagct ttcaaagcgg ggaataacgt
     5881 agagattgtt tttcctgttc cggtaggtgc ttctacaaac aatttctgtt tcgtacggat
     5941 cgttttataa gcagctaccg ctagttcacg ctgacctttc ctatactcat catgggggaa
     6001 tttcaaagcc atcaaagagg cattgcgtac agtgcgccag ttttcttgaa aaacaagcca
     6061 ttctttataa tcttcagtca gctttttaaa aaaagtttct aactgctctc ttgtctgatg
     6121 ttcaaccttt ctcgtgatca cttcttctgc cgtctggaag taagtcaatt gcagatttat
     6181 ttcttgtaaa tcatgttctt gactatagat ataggcatac acccttgctt gatgaaagaa
     6241 aagttccact tgttctggtt ctaaatcttc aaatcgtggt tcggaggttt tgatttcatc
     6301 tatataataa ataccgtctc ttttgaaaat accgtctgca cggccttcaa cgatcaggta
     6361 atattcatct aattgaactg ttgtttttaa aaagacttct ttttcatagt tttctcccgc
     6421 atctttttgg agcttgcggt ggattctcgc tccttctaat gcagtatggt tacttttttt
     6481 acgattgtcg atacttccac gtcgcaaaat aaattctact aagtgccgca cagatatttt
     6541 tgactgtttg atcatttcca ccacctcttt ctctctaaga ttacctgaaa aaacctagtt
     6601 tagcaaaaag agctctcttt ccaaacataa aaaaacaccc tgctgattag gcagggatat
     6661 aggatataga atttggattt ttggatgaat ctaatgtaac aaggacatat taaaatgcta
     6721 tgaaaactta ctttccttct ctttagaata tccaaatact tttcatatag tctgataatt
     6781 cagataagta aagagaaata agtacctgtt tttgtttcct agcaatgaat gcgttatact
     6841 aatggatgaa agaagttttg gctatcaagg aggaatgcac atgcatatcg aaagattaaa
     6901 aacaggaatg attgaagaaa actgttattt ggtctacaac gaagaagcac ttttgatcat
     6961 tgaccctgga gaagatgcgg aacgaatcaa acaagaaatc aaaaaaacaa accaacagcc
     7021 ggtagcgatt ttgcttaccc atactcacta cgatcatatc ggtgcagtgg aacctttacg
     7081 cgcgtattat caaatcccag tttatgtcag cccactagag caaaaatggc taggtgatcc
     7141 gattttgaat ctttctggac tcggtagaca tgatgatatt gctgatgtta ttgtctcgcc
     7201 tgcagaatat gaatttgaaa tgaaaactta tactttagga aacatgtctt ttcgcgtcgt
     7261 ccctacccca gggcattcta taggaagcgt cagctttatt tttgatgatt ttgtcgtttc
     7321 tggcgacgcc ctgtttagag gaagtatcgg tagaaccgat ctgtatacag ggaatttaga
     7381 acaattactt catagtattc ggacccagct gtttgtatta cctgatgaat ttgcagtcta
     7441 tccaggacat ggcgatgcaa cgacgattga acaggaaaaa aggacaaatc cttttttcaa
     7501 tcgttaaaac gacttaggag gagaataaaa tgacagaaac tacactttat atcgtacgac
     7561 acggaaaaac gatgttcaat acaattgaac gagtacaagg ctggtgtgat acacccctca
     7621 caaagcaagg acaagaaggc attcattatt taggtcgcgg gttaaaaaat gtcgactttt
     7681 catttgctta ttctagtgac agtggtcgtg caatcgaaac tgcccgtatt attctaggag
     7741 aacattctaa gggaaaagaa attccttatt ttattgaaaa aaggattaga gaatggtgtt
     7801 tcgggtctct tgaaggtgga tatgacatgg aaatgtgggg tgtgatccct cgtgtattga
     7861 attttaaaac atatgatgaa atgtttacca cagaagtttc ttttgaacaa attgctaatg
     7921 ctgtatacaa cttagatacc gctgggtggg cggaaactta tgaaacattg cgagaccgtg
     7981 tatggacagg atttgaagat atcgcccatc actgcgaaaa aaatggtggt ggaaatgttt
     8041 tagtcgtatc tcatggtttg acgatcgcct ttttgcttaa tctgatcgat cctaaacagc
     8101 ctgttcgtgc tgggctttta aatggcagcg taacaaaggt cacttatgca aacggtcgat
     8161 tttctatcca cggcatcaat gatacgtctt atatcgataa cgggaaaaca catagtcgcg
     8221 actcgtttct ataatgggga tattttaatt tttaccagag aatcataaaa ggcaaagtct
     8281 gaacgagggc tttgtcttct tttttctctt cgtgtactta tctgctttat ttctcaagtt
     8341 tttcttaata ttaaaaaaca gattgggttt ctcaaaaaat aacggtatac tataagatgt
     8401 atcttaattg ggaaggaagg aatttatgtt ttttgcaaat attatcaaag cagtgattct
     8461 cggtattatt gaaggaataa cggaatggct gcccatcagt agtactggtc atttgatcct
     8521 agcagatgaa ttcatcaaac tggatgccag ttcacaattt atgtcgatgt tcaatgtcgt
     8581 tatccaatta ggggcgatct tggctgtcgt cgttctttat tttcacaagt taaacccatt
     8641 ttctccaagt aaatcaacgg tagaaaaaaa agatacgtgg acactttggt ttaaagtgat
     8701 cgttgcttgc ataccagcca tcatcattgg tttgcctttg gatgattggt tagaagcaaa
     8761 tttccacaag ttcttaccag ttgccattgt tttgatcgtt tacggtattg cctttattat
     8821 tgtcgaaaaa cgcaacaaaa acaaaacacc tcgttggact tctctgaatg actttacgta
     8881 ccaagcagct cttcttgttg ggttattcca agtcttggca cttgttcccg gaacatcccg
     8941 ttctggcgca acgatcctag gatctatctt gattggtgct tcgcgttttg tcgctacaga
     9001 gttttcattt ttcttaggga ttcctgtcat gtttggcgca agcggcttga agatcgtcaa
     9061 atatttagct gaagggaatt cattcaaaat ggaagaaact gttgttcttc ttgtaggaac
     9121 attcgtatct tttgtggttt ccgtcttagc tatcaaattc ttactaagct atttgaagaa
     9181 aaacgacttt accgcttttg gctggtatcg tattatcctt ggtatcattt tgattggtta
     9241 ttggtttatt gcaatgtaga aaaaaccaga acacttctct tatttgagaa atgttctggt
     9301 tttttatttt ctttaataat aatcattata aataataaaa acaacatcaa gcttaatcac
     9361 caaatgataa ccattatcgt tctcatttag ctgttattat tatcaattac atttttatta
     9421 tgctatcctt gttttgacaa ggaggtctaa taacgatgac taatcagaaa aaagcttttg
     9481 cagcaacaat cggctgcctt ttatttatga tcttcggttt tatcttcgac cgattctctc
     9541 ttaccatcac cccacttttt tatatcagtg ccattatcat cggtggatat aaacaaacat
     9601 cggaaggctt gaaagaattg tggaatgatc ggacactgaa tgtggattta ttgatggctt
     9661 tagctgctat tggtgcctgt ttgatcggta attactttga aggagcaatg ttgacattca
     9721 tcttttgttt aagcggtgca ttagaggaat acacaactaa taaaagtcaa aaagaaatta
     9781 ctgctttgat gaatcttcaa ccacaaaaag cccaattatt aaaagaaaat ggacaaatgc
     9841 aagaagtaga tgtttctcaa ctaaaggtgg ataacttggt atttgtcgct aaaggagcag
     9901 ctgttccgat agatggcttt ttagaaagca cacaggcaac gatcgatgag tctgcattaa
     9961 gcggtgaatc tgtgcctgta gaaaaatatt taggcgatga gatgtatgca ggaacgctga
    10021 atcaaggaaa tcccatcacg attcgagtaa caaaaacgag ttccgaaact gtctttgcta
    10081 agattatcca attagtagaa gaagcgcaga atacgccaac tcaaacagct tcatttatcg
    10141 aacgaatcga aaataattat gtaaaattga ttttgcttgc tgttcctcta atgatccttc
    10201 tcccccactt tttcctaggt tggagttggg atgaaagctt ttatcgagga atggtattat
    10261 tagttgtggc ttctccttgt gcgcttgtcg catctgctac accagcttct ctagctgctc
    10321 tttcaaattc tgcaaaaaat gggattttaa tcaaaggcgg tatccattta gaaaagcttt
    10381 ctgaattaaa agcgattgcc tttgataaaa caggaacgct cactaaagga aaaccagttg
    10441 taactgacag ctttttcttt gatgaaaagg atttggcaca acaacttctt gttgctatgg
    10501 aacaaaaaac gacgcatcca cttgctcatg cgattttgca atatttcgat tgcacaatac
    10561 ctgaagaaat ccaatactta ccaatagaag aaattactgg ttttggatta caaactacct
    10621 atttaggtgc gcaatggaaa gttggcaagc acgcatatga tcctgagacg atgataatca
    10681 gcaaagagat tgcggaaatg atcgaacgtt tggaaaatca aggcaaaacc gtgatttatc
    10741 tttctaaaga tcagcagtta attgctgttt taggtctgct agatattcca aaagcaaata
    10801 cccaacaagt aatttcctac tttaaatccc aaaacatcca tacaagtatg ataaccggcg
    10861 accattctgg cacagcaaag gccattgctg aacaagtagg gattgaacat tactatgcaa
    10921 gctgtactcc ggaagaaaaa acacaactag tcaaaaaaga gaatgaactt taccaagtaa
    10981 atgcgatggt tggtgatgga gtcaatgatg cgcctgcttt agcagccgct tctttaggtg
    11041 tcgctatggg gcaagggaca gatgccgcta tggatatcgc agacatcgtc ttaatgaaaa
    11101 gcgacttaaa taaatttgtc tacagtcatc ggctatcttt aaaaatgcgg cggatcatca
    11161 aacaaaatat tattttttcg attcttgtta ttctcttgtt gattgcttct aacttcctgc
    11221 aatttttaaa tctacctctt ggagttgtag gacacgaagg aagcacaata ttggttattc
    11281 tgaatggctt gcgtttacta agacctttac ctgatctttc tgattcttca aaaaaatgta
    11341 agtcttgtcc actatacaaa gcgcggctgt cttctgtcaa ataagcaaag tgttttttta
    11401 tgattttgtt tattttatga aaaaaacagc ttatgaatag gtcttgaagg atgaattcct
    11461 acttttgtaa ctttctaaaa acatgttatg attgtttcga agaaagtgag gaatatattt
    11521 catggaaagt tggctttttt taggaattat tttacttgta gcaattttag ctaaaaatca
    11581 aagtctgatg atagcggcag cttttgtttt gattatgaag ctcataccag cctcccgttc
    11641 ctttttccct tggcttcaag gcaaaggaat caatattggg gtgacgatca tcacagcggc
    11701 tattctaatc cctatcgcaa caggagaaat cggtctacgt gatctattag aagcctttaa
    11761 atcccctatt ggctgggtag ctgttttttg cggtgggcta gtggctgtct tatcttccaa
    11821 gggtgtagga ttgattgctt ctgatccaca aatcactgtt gcacttgttt tcggcacgat
    11881 tttaggtgtt gtcttcttaa gaggaatcgc cgccggaccg gtcattgctt ctggtatcac
    11941 ttactgtatc ttacaagtat ttgctttagg aaaatagaac aaattttgtc tattttagga
    12001 gattcttaac acccccaatt tactgatccg tgatatactg acaacgaggt gatacaatgt
    12061 tcgaaaaatt aaagtatttc ataggaacaa tcggctggtt tcttctagcg gtacttttga
    12121 ttctgggctt agcttatcta ttatttcctg atttgataca aaatgtgttt gatactgctt
    12181 tttgattgac ctattaaaac gatatagggg atgctcctgc gatgcagggg ctttttttaa
    12241 tcaatgtttt gtaataattt aggccgtgcc aatgagactt cctatagaat tcgatgctct
    12301 tttttctttt tggctcttct gctctttttt gttatactag attacagtgg acaaaagaaa
    12361 gaatgatcgc atccacttca acgaatatca aaatggaaga attagtgcct gttttcaggt
    12421 tactttgaaa agaaaggaag acgcttgtgg aaacttttga aggtctttta attgatacgt
    12481 acgttttaca agaacagcca atgatgctga ctgggatcaa cttgcagctt gaggaccgtg
    12541 tgatcaatgg cctgcttaaa gatcgctatt tggcaaaata tatcttacgt ttgcccctaa
    12601 ataaatatgt gctggaagta agtggcaaat ggaaccgtcg gaaccagttg gtcataaaaa
    12661 aaatgttgat aaaaaatatc gatgaataca ttaaaataat cggtacacca gaatagcctt
    12721 tactgatcaa aggatactgg ttataaaata actgcagttt cttaatagaa aaacaaagaa
    12781 caacagggaa tcgtatccct tttcaaaaaa aactttgcaa acggttctct ttgttatata
    12841 ataacttagt accaaacatt cccattcaaa ctttgtt
//
LOCUS       DAHHZJ010000071        12797 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.072, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000071 DAHHZJ010000000
VERSION     DAHHZJ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.072"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(26..328)
                     /locus_tag="NBL02_001609"
     CDS             complement(26..328)
                     /locus_tag="NBL02_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCD2572294.1"
                     /translation="MNKLNILFVCGAGLGSSFAEQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(353..2446)
                     /locus_tag="NBL02_001610"
     CDS             complement(353..2446)
                     /locus_tag="NBL02_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCD2572295.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(2935..3699)
                     /locus_tag="NBL02_001611"
     CDS             complement(2935..3699)
                     /locus_tag="NBL02_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HCD2572296.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="NBL02_001612"
     CDS             complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="NBL02_001612"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HCD2572297.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHDASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(4704..5780)
                     /locus_tag="NBL02_001613"
     CDS             complement(4704..5780)
                     /locus_tag="NBL02_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCD2572298.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(5804..6397)
                     /locus_tag="NBL02_001614"
     CDS             complement(5804..6397)
                     /locus_tag="NBL02_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HCD2572299.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="NBL02_001615"
     CDS             complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="NBL02_001615"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HCD2572300.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(8067..8366)
                     /locus_tag="NBL02_001616"
     CDS             complement(8067..8366)
                     /locus_tag="NBL02_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HCD2572301.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="NBL02_001617"
     CDS             complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="NBL02_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HCD2572302.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(10121..11032)
                     /locus_tag="NBL02_001618"
     CDS             complement(10121..11032)
                     /locus_tag="NBL02_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCD2572303.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            11152..12009
                     /locus_tag="NBL02_001619"
     CDS             11152..12009
                     /locus_tag="NBL02_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCD2572304.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(12196..12558)
                     /locus_tag="NBL02_001620"
     CDS             complement(12196..12558)
                     /locus_tag="NBL02_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCD2572305.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
BASE COUNT         3603 a         2719 c         2223 g         4252 t
ORIGIN      
        1 tttttaccct cttttctttt tgtatttaga taactccctt atcttgcaga actggggtga
       61 ttttttcttc aatttcattt tttgaaacaa tatttttcaa atagatcatc gccgttttag
      121 gatcaactgc gaacttttcg aactgcgaac gaaaattttc tgctgtgatg atcacatctg
      181 gtttcatgga aactgcggaa gaaatatcac aatggtccag tttggcttct actcctaatt
      241 tgttcaaaac atcctcagca gacatctgtt ctgcaaaact acttcctaaa cctgcaccgc
      301 atacgaataa aatgttcaat ttgttcatct ttgttctccc ccgtatcatt tattatttct
      361 caactactga aaaaagcagt tgtttgaaat cttcgatatt tgtgcattca atcaatcgat
      421 ccaacttctc ttcgttgttg atcaattcaa ttagttcttt catgatgttc agatgcgagt
      481 aggagtctac tgctgccaaa caaaaaataa tctttactgg atccatttca ggattaccga
      541 aatcgattgg ttgctcaatc gtagctacgc tgactcctaa tttattgacc ccatcttctg
      601 gtcgggcatg ggctagtgcc atgtgtttgc caatcacgat atacggtccg taatcattca
      661 ctgcacgaat catcgcttcg acatagcgct cttcgacaat gttttcttta accaatggtc
      721 tagcagctag ttcaatcgat tcctcccaag tagtggcttt ttctttgatc atgatattgc
      781 tgtccgtcaa aatatctttt aacataggct ggatctccct tttgttgatt tctaaattgt
      841 ttgtttcgaa taatttttct aacttgccat agatgattcg atctactcgg ccaccacttt
      901 ctttcatgac atctattatt tgagtgaata actccgtcga ttcatgactg ttaggctcta
      961 accgctgtag ttcttcattt cttttcaaaa agtcttctat aaccaagata ttcgtctcag
     1021 ttaaaatcgg atcgacaact aaaagcggct tatttcgata attcaattga aaggtgctga
     1081 aaaccagatt gacatccaat ttatctacta attcgatttc ttttgaactt aaaactgcag
     1141 taatctcgat ctgaggaaac ttttctttca gattctctgc taacagattt ccagtagcca
     1201 tcccatggtt acagacaacg actgatttat aaacataatt caagtctcgc ttgattacac
     1261 tggcgatcgt cgaaaagtgg atgacaagaa aagctatctc gtcctccagt atcttgccac
     1321 caattacttc ttcgatcgta ggaacaaaat gagtgactgc gcggtagatt gggaaataat
     1381 ttgttcggat gttttctttc aatggattca tgacttggat atgattcttg attcttacaa
     1441 tcagtgcagc catgtgctta tagagatttt cgaataaagc ttcttccttt agatggaaag
     1501 gtacgtgtgt ctcgttctct acaaattgga tcaattggat cgttagtaat tgagcttgta
     1561 cccactcgat tgaattggag atatctctag cattgaacgt ctcaatagtg aaacgaagat
     1621 aatttctttc tacttgcggg acattgtcca attgaaattc ctcgatcatg tggtcaataa
     1681 atgcagaaaa gctactgtct tttatttctg tatttttcca attaatggca gagataaatt
     1741 catgtttcct gattcgctgg atccagatca atgtaaaaag caggatttgt tttttataaa
     1801 tatcatcttc ttgtttcaac aacttattgg aatagatctc ctgtattttt ttgatttcca
     1861 ccaaaggaaa ataatggaga aagatttttt gagccggtgt taattgtgca tcttgattca
     1921 taaagaagtc ggtcattccc agattctgat tgataaaatc atagatcatc gtacggatag
     1981 atcgttctga acctttataa accagtcctt gctttccata actgacgatc tctatcccat
     2041 aactgaccag tgttgctcgt aaccgtctca tatcttcatc taatgtactt ttagagattt
     2101 gaaattcttc ttcttttttg tttagatgga tcgggattcg gctgtaagca acggctaaaa
     2161 ctagatcaaa aatacgttcg cgccgattca aaaagccgtc tttcacatct tttatttcag
     2221 tttctaatcg agcttgcttt tccttttcca gcttcaacaa aaaacctttg ttgcgaattg
     2281 tacggatctc cggaagcttt ttactgctca accagatatt cgcttcaaga acttcctgac
     2341 ggatcgtccg ctgactgaca ttgaactcac ttgctagatg ttttgctttt actggatatt
     2401 gggctgtcat gattctgtga agtatttcaa ttgttcttgt tttcatagaa atgatctcct
     2461 tctgatttca agtatatggt tccgcttaca taataaaaag tagatatttc tgccacttga
     2521 agtggaaaga aagtaagcaa agcaaacaaa aaataagcca tatgcttttt catctcctca
     2581 acgaaaaaga catacgccta ttattacaaa aattttgccc atctatacaa aaaagaggtt
     2641 gtgaaaaagc tgtcctgcat caagtaataa gaacaactaa actaaaatag tgcttcctat
     2701 ttttcgtttg gttgttctta ttatcgcaga tgcaagcttt taaacaccat ttagtggaga
     2761 ggttgtgaca gagatgttca gccttgagcg ataagaagcg attttgaaaa atcgcttccc
     2821 atattttaca aaatcgtgcc ttattgccga taggctatct ctggcacacc gtttattcag
     2881 aaaaaggagc tgcgacaagg cttttgtcac aactccgttc ataaatattt cctgtcaatc
     2941 cagcataatc ggctgaataa aatcggcttg ccatcttttt tctttctcag aaaaatcaaa
     3001 ataccatatc tgagaaaaat actgtcgttg tgtcttactt cctgagatca atcgcccatc
     3061 ttttcgatcg actgaataat cacagcatct gaaatcaatt cggatggcaa aactattttc
     3121 gtggatctgt cgataatttc ctacttcctg aatagttatt tttttagtat gatttcttac
     3181 accgtctgct ttgttttttt gcagaacttt ctgatgttcg gcaaacagct tgtctgtata
     3241 acagcttctg gctttttcag gagtttcttc ttcccaagcg gattggatct tcatgaatat
     3301 cttttggatt tccagaaact gtgctttctt ttctttagcc gttcctgaca tgcgggacaa
     3361 tatctccgct ttcagccttt tcttttgtct tttattcttg acgatagaaa aagcagaaac
     3421 ccctcctact gacaaaacag ctaatgcaag aaatcccgtt tgctcataac tagaaggacc
     3481 tgtacctgga ccataatacc cataagaatc tctgccgtaa gaacgactgc caaaataact
     3541 gcccccatat gaacgaccac caaatgaacc tctgcccgaa cttcgaccgc tagatgtacc
     3601 tccaccatga ccacccgcga cagcgaatcc ttgggtcggc agagcaaaca aacataggcc
     3661 cactaacaaa atgattgcta aataattgat ttttttcatt gtcttcctcg ttatctttta
     3721 ttacaaaaaa ctatttttat gatagccttg ctaaaaggaa gagtcaataa ccagatgcat
     3781 aaactgtttt tttagcaatt ttgtcttatt tagtcattga gtaacatcgg acaaatgtat
     3841 gtgtcggcag tccataattc ctctatcttc agcatgaaca agcttgcaga caagagatct
     3901 gcgcttcctc caggagagat ttggttttcc tgacaaaagt gcaacagtgc tgtaaatgga
     3961 cgatttggta aagcactttg actagcatca tgagcaagtt tttgcatttc cactaacgtt
     4021 ttctgatcac tgcgatgcaa tacgcatgtg tcatctagcg aactcatcaa aactaacaac
     4081 atatgcattt gtttctgtac atatgaatca ttatctagct ccttagctgc ttgaattgca
     4141 agcctgatat gcggataccc taatatcgct tcttcatatg caccatttac gccatatttt
     4201 tgcttcaccc tatgaccatg agtggttttt ttatgacgat attttgtatc aggaaaagca
     4261 gcaagcttgc tgacatcatc aagtattttc gataaagaaa aaggctgttc catactgatt
     4321 ttttgtgcaa tgacactgat caatagcccc attgcccaaa tcgctccttt atgggtattg
     4381 atgccatttg tagcaatcag cattgccttt tccgcttttc gcccaatctc agcaattctt
     4441 tctcttaact cctgatcaat agcatgatgc catgctattt gagccatttc ttcaaaaaaa
     4501 ggagttagcg tcttagcaga cttaagaaat aaagcatagt ccatatcgga atgtgcaccc
     4561 gcatccagtg catccactaa tccaggtttt ggacttaatg ttacttctgc gatcaatgct
     4621 tcctctacca acgcagcgag ttgctgagtc actgcttttt ttcgttcgtc atttatcatg
     4681 acactcactc cttcttccta ctattacttg ctgaaacctt ttttgatagc tacccaaatg
     4741 atatgaagta accagcttaa agcaaatgca caacttgccg tcattagata agctacaaga
     4801 atcatgactc ctgaaggtaa ctgcagccaa ctatctttaa acaataataa cagtacttgg
     4861 aaaacaaata cattggctaa atacgtacga taagcataaa cagacaacca tttgaacaat
     4921 ggcaattttg gcgaatcgtt tacaatcatt ttggaagcgc ctatgaagac agcaaagatg
     4981 atgactaaac tgtataaact ttggatcgta ttcaagtaag gtgcattcgc aaagctcaag
     5041 tctcctggat gtgcgagcaa tgaacggaca gaaaagaacg cgataactaa ggcaacaggt
     5101 aagaaaagat aacgtactct gtccaaatat tgatagactt tttcgtgata aacaaatgcg
     5161 gctaccccat aaatcatgaa actagcaaca aaccgatcta ataagtacca gctgtcatga
     5221 taaggcccag tcaatacttg tgtttgatag aacagatacc atgcgacata taaaatcgtt
     5281 gaaccaatca atactggcca tacatatttt ttattagcaa atactttgta accgagccaa
     5341 acaaaaaacg gcatgaacaa ttggatctgc agcatcataa cggtatacca taaatgaggc
     5401 gctccatcac ctgtgacata tttcagtaaa aagcttgcag tatccgtata cggagtttgt
     5461 tgttggactg ctggaaatag ccaaagataa gccgtcgtcc atagaagata cggaacgact
     5521 agttcaaaaa acttttcttt caaaaattcc caataagaca aatgctgact tgtttttacc
     5581 atattataaa caattgcaaa aataaacatt ggtgctgtgt atttaacgat gacgtaaaag
     5641 gtaccaagaa aagctgtatt tgttgcgctg tggctgtttg ccaaagcaaa gctcaatatc
     5701 gtttgcatca taacagccgt tacagctgat actttctgga agtcacttac gttgattttg
     5761 atttttttgt tttcaaacat ctctttctct ctctttttct ttattaccaa agttctttgg
     5821 ttaatttagg tccttcgact gttttcaata gaattttttt ctctggctct ctcaaatact
     5881 cttttagtga aaaaccgcct tgccgagtca ctacttgggt atcgaattgc tcaaaaaaag
     5941 caggatgcga ttgaattacc tgaattggca attcaatcgg tgaatttgcg tacagcagca
     6001 aatcaaaatc actggtttct tttactgccg gaatcccagt cgataattca aatcctaagc
     6061 tgccgccgat tccccattta catcctgata atatgtttct agcagcatga aattgctgat
     6121 aaacaggata atccatgatt ttctcttgtc tatacgattc ttgttcgatg atgtcccacg
     6181 gatgaatgac ttgtttgacg gcatgttttg agatttctgt tgaccatctc tgattgcggg
     6241 caggtccacg taaaccaatc ggtagtattt ctgcgtgatc cgtcgacgca cgacgaactg
     6301 ttccgaacat cttttcagtt agaacgaccc attcaggcaa ggtgaaccct tctatttcat
     6361 gtttatcaaa aaggacaata tcatgtgctt gaatcatttt ttatgcatcc cattcttcgt
     6421 ccattttggc acggacttta tttgtttcga cacgtccgaa tgttttggct gtttcgtttg
     6481 tgtatcgatt tgtcagcatc gtgtcatttg tattgcggac atcttgaatg gcttcattga
     6541 ttgcttcgat gactttgctg acattttgtt ctgaagcagc ttggtcggtg atatcttcta
     6601 ggaaatgata gatcgctcct agttttgtat aattattgat atcgtaagcg atagctggaa
     6661 ctttttcagc agctgcttcg atttcagcaa ccgaacgttt tgtcacacgt gcagcacttt
     6721 ctttagacat cgcttggacg gtaatttctt tattgtttaa agcaatcaag cggcttgatt
     6781 gtaatccatg tgctaaaaat gcacctgaaa tagcatttcc tgcaatcaat ccaataactg
     6841 gatgtccagc ttgacggagt tttgcataag cagcagcact attagccaaa gccacatgga
     6901 taccgatcaa ttcttcgtta tagccgtatg cttggcttgg tacatcgatc acaacgatga
     6961 ttggccgttt cacttttttg tctttatctt cttcgtaaac gtgatccaaa atttgtccca
     7021 tgcggaaacc ttcaactaaa ccaacttcac cgtttcgtac acggtacatc gggtttttag
     7081 gatcaggtac gattgatgtc acgatggctt cttctatgaa tttacttgat ttaccataat
     7141 acacagtact aatagaagaa gttggatttt tcatgccagt tagtgccgca aaccaatcgt
     7201 aaccaacact tgctgcagct ggttcatttc cttctgctac ttctggtaag tcaattgatt
     7261 ttgcttgatg gttagcaatt gcttcattgt aatcgtcaat ggccattggt tttgaaagat
     7321 ctaaagaatc aagtaatgat aaataaaatg ctgagctttc actgcgatgc gtgtcttttc
     7381 gttcgtccat tgctgcgatg acagcagctt tgattgcttc tacactatca gtcactaatt
     7441 catcaatgat ttttgattct acccgttgac gagatcctaa tgtattccaa atcaagtctt
     7501 tgtcactaga atcaaattct cgaacgcctg cttcttgttc aataacttct ggtccattca
     7561 aaccgactct cgcttttttt gttgcgatta aataactcat taatgctgaa gtaatcgaca
     7621 taccgccaaa tgatcctaca cgtcctggaa tcaaaccgat gactggtaca tattgtttta
     7681 gagcaatgat tacatttccg atttctgaaa tagataacag tccgtagttt gcttcttgca
     7741 aacgaacacc acctgtatct agtacaatga ctggaaagat ctcatttcct tttttatttt
     7801 cttctaacac atgttcaagt gcagcaacga tttttgcacc ggaaacttca ccgataccgc
     7861 caccttggaa ttttccttcc atagcgatta ctactgcttg tttgccattg atcgtgccac
     7921 ggctgacaac gatcccgtca tcactttcag ggacgattcc ttgtgctggt aaacttggcg
     7981 cgatgatttg atcgaatgga tcaatcaatt cacgtccatt gtcaagtaga gcgttcactc
     8041 gttctctggc gtttaattct acaaaactat ttttcatctg aaagttcctc cattgcttgc
     8101 gtcaatcgta ggtacacaac tcctggtgta gcaccgaagt cattgatcac gatatctgct
     8161 gaaattggat aacgatcaaa aaatctcgtt aatacattgt tccatacttc gccgaagcca
     8221 tcgcttcctg tacaaatttt tacagtagat tctgtttttt ctgttggaaa catcaagatt
     8281 tccaaatctc ctgaaccgac cactcctaca tggattttct tattgatcgg ctgtttcgtt
     8341 ggatatttat aagctaattg ttccatggat ctctcccctt tttgtaaaac agagcataca
     8401 cgttctgctt attcgtcttt cttcttccat ctgggtaaat ggcgacaaaa ggtttttctc
     8461 gccaatttta tgaccaagtt ctgaatttca ttggtggttc atacaggcca tctgaccatt
     8521 cgatcaaatc atcgatggtt tgtgcagcta ataatgaacg tttcgcttca ctacgtttga
     8581 tgcctagatc ttctggtaat ttcacgatgc cggctgcacg caagctatct agtgattctt
     8641 tcgtgctagt cattccaaga ggtgtaacac cgccaattgc tgcaattgct gcttgtcgtt
     8701 cttcttttgt ttttgctttg tacaaataag caattccttc ttctgtcacg atatgtgtag
     8761 tatcttcgct ataaatcatc actggtgcat tggctaagcc tgcttgtttt tgaacagcta
     8821 gtgcatctaa ttcttcgaca aagactggtt ttttatttga accatatgtt tcaaccattt
     8881 gaacaactaa tttttggcct ttccctaatg gatcatcatt gtcgcgaagt gattgccatg
     8941 ctggtgtaga atgacgacga ccgccaggat tatgtcccat gttaggcgca ccgccaaaac
     9001 cagataaacg tccttttgtt acggtagaag agtttccttc gtaatctaat tgcaaggttg
     9061 aaccgataaa catatcgatg gcatattgtc ctgcagcttg tgataatgtt cggttactgc
     9121 gcatcgtgcc gtctttaccg acaaaaaaga catctgggcg agcagcaata tatttttcca
     9181 taccaacttc gccaccaaaa ctatggatgc tttcaaccca acctgattcg atggctggaa
     9241 tcatcgttgg atgtgggttc aatacccagt tcttcgcgat tttccctttt aaccctagtg
     9301 attcaccata agttggcagt aacaattcga tagctgctgt attgaatcca ataccgtgat
     9361 tcaaagattg tacgccgtgt tctgcgtaga ttcctttgat tgccatcatt gccattagaa
     9421 tttgcaattc tgtgatgttt tgaggatctc gtgtgaataa agcttctaat tgataaggtt
     9481 tatctgcttt cacgacaacg tctacccagt caccagggat atctacacgt ggtaaagaat
     9541 cgacaacttc gttaacttga acgatcacga taccatcttt gaaagctgca gcttcgatga
     9601 ttgttggtgt ttcttccgta ttgaaacctg tgtacaagtt gccgtcttta tctgctttat
     9661 ctgcagctac taaaacaaca tttggaatca aatcgatgaa taagcgtcca taaagttcca
     9721 aataagtatg gatatcacct aatttcattg tgccgtcttc aatcatttgg gcgacacgca
     9781 agctttgtgc accagcataa gagaaatcga tcttgcttgc gattcctttt tcaaaaatat
     9841 tcaaatgttc tggtcgtgag atactagaca tgatcaaatg cagatcattc actttttctg
     9901 catctacttt ttcaagactt tctgaaagaa aacttgcttg tttttgattg tcgccttcta
     9961 atacaacttt gtcgccggga gcaatcagtt tttccattac ttctacgatt ttttcagttt
    10021 ctacatactt gccattgagc aagttgctga ttttttccaa tcgctgcgct tttttttcgc
    10081 gtttagaagc ccagttgcgg tttggttttt ccataattag tcatctccat ttttgatata
    10141 gaaacgtggc gtcatcaata ccatgttggt ctagattgat cgtacggatc cctttatcta
    10201 caccaaattt tgtatgaatc agctttgtca aagtattgcc ggggggaaat tcaatcgtca
    10261 gatccattcc taattctttt gccaagtcca tcatttggtt ccactgcaca gggaaagcta
    10321 ggttttccaa caaatcttta cgaatctctt ctgcgtcctg agtggctctt ccatccgtat
    10381 tttttatata caaacatcta gcatcagata gatggacaga ctgcatatac ggcttgaatt
    10441 tcttgaccgt atcattcatc aagagacagt gagatggcac agggactttc agcagcttcg
    10501 ctgtttgcgc ttggtgccgt ttggcttctg ccaacgtaac ggataaacct tcgatactcc
    10561 cagataacgt atgttgcatc ggacaatttt catttgacag ataaactgga tggtcaggat
    10621 caaatgtttt tgctacaatt tcctcagctt cgcttctcgt cacacctact actacgccca
    10681 ttgcataacc gcttggataa gcttctttca ttaagactgc gcggtgatag actagttccg
    10741 ctgcattctc aaatctcaaa gatcgacaag caacagctgc acaaaaagca ccaatcgaat
    10801 gtcctgctgc aatatcaggc tgaataccgt ttttttccat ttcatctaaa taaaatactg
    10861 cttttgtcag taatgctagc tggataaaaa ccgtatcttg atagttttct tctttatcaa
    10921 ctaaccgata ccccgtcaca ttttctaaac gttccatgta catttcaggg atatccgcta
    10981 acatccctaa cttctgactg ccttgtccag gaaataagaa acctgtactc atgagcctcg
    11041 cctcctcagc acttactttt tccactgcta tcttagtcaa tttctgctat aataaaaaat
    11101 gccaaaaaaa gaaaaaacca taacaaaaag gtatggatgg gatggataat tatgaaattg
    11161 cttcatctgc agtattttaa aaaagtattc gaaacaaaaa atgttaccca agcagccaaa
    11221 gaattattta tttctcaacc tgccttgagc cgtgcgatca aacatttgga aaacgaatta
    11281 ggtgtccctt tgttttacca tagcggcaga aacattgaga cgaccgttta tgcagaagaa
    11341 ttttatccat atgcagtcaa gacattagac acattagaag aaggaatcaa attattgtct
    11401 tcggtcaatg agaagatcgt tacttccgtc atattgtatt tagaggtagc ctctgtttct
    11461 attccgaact tggtccgaat ttttatggaa aagcatcctg atatccaatt aacgatcatg
    11521 caacatcatt taccagatga tattaccgat cctgtactgt acatcacttc ggagatgaag
    11581 cctggcatga ccaatattcc tatcatgaaa gaacctgttt atgtcgccat tcctaagaaa
    11641 catcctcttg ctaaaaaaga acatctgcaa ttagaagata tccaacgcac ccccatcttg
    11701 atgctgtcaa agagcaacgc cttccgtcat acactggatg cagcacttga agcgaaaagt
    11761 atcgaattga cgatcagttc gacgacagat gatcctgcga cattacgttc tattgtcaag
    11821 caaggattag ctgttagctt tttcccaaaa gtttcttggt cttacgataa aaatgatcct
    11881 ttcgtcttgc gggaaatcaa agatttgccc ttgacacgaa caatttattt atcttctagc
    11941 ttcacagaag atcatccttt gatcaaaatg attgcaaaaa cgatgcggga attttattta
    12001 ggaaaataaa aaaaaaagag ctgggacaaa attcttgtcg cagctctttt ttcgaacaaa
    12061 cggtgttcca gaagtaattt cttagaaaat aagccgaaat ttcccaaaaa tttgagaagc
    12121 aattttcgta aattccttct tatttcttga agctaaacat ttctgtcaca acctcttctc
    12181 tattgtccta gacattcatt gcgttgattt aaaacttcca tcaatcaaat ctgctagttc
    12241 atctagcttt tctattttac gtagatcaat agagatccaa tgttctttat tcatgtgata
    12301 ggctggaaaa tacccctctt ttcctcgcaa aattccgcca agttcaggat cgaccttcaa
    12361 atcaagaata ttgatgatcc tatggtcact tgcatcatca tagatttttt cttctggaac
    12421 agacatcagg accccaaacc attttttgct tttttgatgt ctgaatacgc aataatcagg
    12481 aaatttttta aaaatatatt ctggagacaa attgtatgtt tcattgatgt gatcgattag
    12541 ttcttttttc gttagcatct gcttccatct ctttcttatt gattcgcttt attcaaagat
    12601 accacgtaag aatagaaaaa tgaatacaag tagttgctaa tcaaatactt cgatattttt
    12661 cttttttatc aactcttaaa tgttcaatcc gcctgtgttg gtcttgctac catctactat
    12721 taaaatgttt attattgttt gcccacctat tttttgattt tagtggtatc atttttcaag
    12781 ttcatgatga aattctt
//
LOCUS       DAHHZJ010000072        12768 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.073, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000072 DAHHZJ010000000
VERSION     DAHHZJ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.073"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(102..785)
                     /locus_tag="NBL02_001621"
     CDS             complement(102..785)
                     /locus_tag="NBL02_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCD2572306.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(803..1420)
                     /locus_tag="NBL02_001622"
     CDS             complement(803..1420)
                     /locus_tag="NBL02_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HCD2572307.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(1430..2255)
                     /locus_tag="NBL02_001623"
                     /pseudo
     CDS             complement(1430..2255)
                     /locus_tag="NBL02_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
     gene            complement(2248..2808)
                     /locus_tag="NBL02_001624"
     CDS             complement(2248..2808)
                     /locus_tag="NBL02_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572308.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            2982..3938
                     /locus_tag="NBL02_001625"
     CDS             2982..3938
                     /locus_tag="NBL02_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCD2572309.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(4243..5199)
                     /locus_tag="NBL02_001626"
     CDS             complement(4243..5199)
                     /locus_tag="NBL02_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCD2572310.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            5303..5905
                     /locus_tag="NBL02_001627"
     CDS             5303..5905
                     /locus_tag="NBL02_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCD2572311.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(5993..7492)
                     /locus_tag="NBL02_001628"
     CDS             complement(5993..7492)
                     /locus_tag="NBL02_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HCD2572312.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(7508..8032)
                     /locus_tag="NBL02_001629"
     CDS             complement(7508..8032)
                     /locus_tag="NBL02_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCD2572313.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            8330..9079
                     /locus_tag="NBL02_001630"
     CDS             8330..9079
                     /locus_tag="NBL02_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCD2572314.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            9070..9855
                     /locus_tag="NBL02_001631"
     CDS             9070..9855
                     /locus_tag="NBL02_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HCD2572315.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            9824..10540
                     /locus_tag="NBL02_001632"
     CDS             9824..10540
                     /locus_tag="NBL02_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCD2572316.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(10642..11676)
                     /locus_tag="NBL02_001633"
     CDS             complement(10642..11676)
                     /locus_tag="NBL02_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HCD2572317.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(11680..12738)
                     /locus_tag="NBL02_001634"
     CDS             complement(11680..12738)
                     /locus_tag="NBL02_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HCD2572318.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT         3713 a         2655 c         2310 g         4090 t
ORIGIN      
        1 aaaaatttct cttttttttg aattctgaac aaatagtact ggagtacaaa agacgaggaa
       61 gagccactat ctgctcttcc tcgtcttttg attctcacct gttattcatg attctttttc
      121 tcagtttccc gatatttttc cattttacga ctaaaaagct ctccgctact tggtggcgta
      181 tatgtgatgg catttgctcc tgcttgaatg gtctcagcaa tactttcttc cgttggacca
      241 cctgtcgcaa tgataggaag ttcaggatat tttttacgaa aatggcgtac tgcttctgca
      301 gtttctttac ctgcactgat attgataatc gaaataccag ctgctaactt ttcatctatt
      361 tctgtatatt tcgacgttac cgtactgata actggaatat caactactcg acaaacttct
      421 tttactgtct ctaaaggcgt aggaccgttc aaaacgaccc ctaacgaacc ttgcgcttct
      481 gcgaacatgc tcatgtagga agaacgagca ccttgtgtca aaccaccacc tacaccggag
      541 aaaactggaa tgtcagctgc ctcaataatg cttttagtaa tcgctggatg aggtgtaaaa
      601 ggatacacag caattactgc attcgcattt gtattacgta tgatcgcaat atccgtagta
      661 aagatgatcg aacggatcaa ttttccaaat atcttgatcc cgcttgcttc taaaatgatt
      721 tccggtactc ttacgatatc ttttctcaat tccgtcatga tttctggcgt aaattgttct
      781 gccatcagcc acttctcctt tgctatctcc ggtcatactg attcaattga tacgtctcga
      841 tgacctggat gattttttgc gcataaccgg ggtctgtcgc ataacctgca tcttgcaatg
      901 cttgtgcggc ttcctgatag ttggtggccg taatcacttt ttcatacagc gcaggattcc
      961 aatctactcc ttggacgaac agttgtgtat ggtcatccat ggactgttcc catgaatcat
     1021 acactttgaa gtctccttgg atagttatcc attcttcgtt aacaaattcc tttgtttcca
     1081 ggcttacttt cttctgatcc ccataagcct tgatcccgaa aagattattg tatttgctag
     1141 ccaaggtact ttttccccaa tttgattcta atatagcttg tcctaaaatg atactaggaa
     1201 gcactccata accatcttgt agttcttggg catgcggaac aagccgttca ataaattgct
     1261 gatgggattg cgcttcattt tcttgctgat tatcagtaaa aggagaagac agattcttca
     1321 aggaaaaaac aaaggctaag agtatcactg ctacaccggc aaccactgct ggccaacgga
     1381 tattcttgcg cttttgcttt ttccactttt ttttcgccat gatcaattcc tacttttcat
     1441 ttaattcatt cagatattct tctagcgtca attggtgttt ggtgatatac tcagcgtttt
     1501 ctttaccgac ataacgtaag tgccaaggtt catatgtgat cttagttata tcttcgtgtc
     1561 cttttggata acgaacgata aatccatact gcgcagcatt atctgcgatc catttagctc
     1621 caggctgatc tccgtaactt gcatccaata cagtagaagg ataactgttg taccagtttt
     1681 gatcgacgac atcgactgcc agacctgtgt ggtgttcgct gtatcctggc tccgtaatcg
     1741 tttctttagt aatctttgtc gcctcctctt ctgatacacc ttgacgactc atcacatctt
     1801 ggacattttg ttcgaaaact tgttgttggg atgataacag accggtaagc tgagaccatc
     1861 actaatggaa atccagctgc ttcagcagcc ttggcaaatg cttcatattc tggagcaatg
     1921 cgtttgtcga tcaagtatcc gttacttaat gatgccaatt gagtagactc atcaatttct
     1981 ttttctaatt tgtgatctgg accaaccaaa actagcttcc aatcgtctgt tttaacatct
     2041 ggcaattgtt tggacgtacg atcttttgtt tctttgtcct ttaccttgct gctggttgtt
     2101 tgactgttct tttctttact agcagttgtt ttggtgcttg ttcgttcgac tgcttgattg
     2161 ccggcttgaa catgcgaatc atcatcgcta aaaataacat agcccattgc tgcgatcata
     2221 atcacaactg ctccaaatcc taaaaattta ttcacttgaa aaatcctttc gttgttcttc
     2281 acgttcccca ttcgcctcat gaacgtaagt ttcttccaga ttttcattca cccaatcgag
     2341 aagttcgatt ttttctcctt cgatctgttt ttgaacatct gctggcagac gaaaattcaa
     2401 aatatcatca tagccccatt catcatattg aatacttact ttcaagtcaa ggtatctacc
     2461 agcctgctga ttatttttca aagggatcag ctcgactttt gctgctttgc tgctaagcca
     2521 tttttgcgcg atgcgtgttt tgatttgctt gtactctgca acggcaacgt ttcgtttttc
     2581 gggaaaaata atcgtttgac gcaaaatctt ttgcgttagc atccattcat aatcagtgaa
     2641 aagatttttt actttctgca tcgcttcaga tggcagattt ttctcgccat ctttccattt
     2701 ttgccattgt tctggactga tcagcaagta ttcatctaaa aaattttttt cagtttcaaa
     2761 ctgttctaat aatacactca taataagatc aatatatact tgatgcacgt ttatcccctc
     2821 cttttaacta ttctacttaa gttttgatca aaaagcacct cttaaatcgc tgctttcatt
     2881 ttttttaatg tttcaagtcc tctcctcttt attcttcgtg atttttgtta taataataga
     2941 cagacaagaa actcaatcca actatgaagg agcgttttca gatgaaaaaa tatcaatggg
     3001 gaatcgttgg tcttggtacc atcgcacatg agtttgccga aagcttcaat caagagacga
     3061 gcgaactaac agcagttgct tccagaactt cagaaaaagc ggaaaacttc gctcaccgtt
     3121 acaatattcc aaaagcttac ggaagctatc aagaaatgtt agacgatgct gaaatcgaca
     3181 tcgtgtacat tgctgtacca aatagacagc atatcgacca tatcttggca gcattggaag
     3241 caggcaaaca cgttctatgt gaaaaagcca tcacgatgaa taaaaaagaa ctggctgacg
     3301 ctatgcgctt agcagaagaa aagaatttga tcttagcaga agcgatgaca atcttcaaca
     3361 tgccacttta tcagcagtta cgctcaataa tggatacagg aaaactgggg gctttaaaaa
     3421 tgatccaagc acctttcggc agttacaaag aaccagaccc taaaaaccgg ttcttcaatc
     3481 cagaattagc tggaggagct ctgttggata tcggtacgta tgctgtatct ttcgctcgtt
     3541 tctttttgag ctctcaacct gaagtagttg cctctacaat ggttcctttt gaaacaggcg
     3601 tggacgaaca atccgtcact atcttgcgta acaaagaaaa tgaacttgct gctgtcagct
     3661 tgacttttca ggcaaaaatg ccaaaagtcg gtatcgttgc ctttgagaac ggttatgtga
     3721 cgattgctga ttacccgcgt gctgatcgtg cggagatctt atttacagat ggtacaaagg
     3781 aattcatcga aagcgggaat acatctgaag caatgaatta cgaaataaag aatatggtac
     3841 acatgattga aggtgacttg cccaatcgct ctctcttctt gacaaaagat gtcatcgaaa
     3901 tcttggatca aatgcaagtt ctatggaaaa atatgtaatt ttaaaaggtt gtgaaaaagc
     3961 tgttctgcat caagtaataa gaacagccaa acaaaaatag ctcctcctat ttttcgtttg
     4021 gctggactta ttaccgcaga ggaaagcttt tgaacaccgt ttaagaaaga ggttgtgagc
     4081 ctaccgttca gctccgagtc acaaggagca atttcaaaaa acagcttctc atgtttgttg
     4141 aatattgtga cttgtgaccg aggagttgac aggagaacac cgtttaaaga agaggttgtg
     4201 aaatcagctt tttgacctga atattagctg aattcacaac ctctattttt ggattttttg
     4261 ttgggagaat tctgccttga tttcaataaa acttttaaaa gactgcaaaa actggaacaa
     4321 ttctgctcga tcctcgaatt cagggcgact agtcggcaga ggcatttgcc gatacaatac
     4381 ataaacttct tcaatacgtg ttaaaatttg tctgccatca tttgactctg aaaatgtttc
     4441 tgcagtataa atcaataaac catagatatg ttttccataa taagctggtt gctggattct
     4501 ctctagattt tccagcatgt ctttaatcac attggcctgt gctcttctca tagagaagta
     4561 agtttcgtag tatagcggct gtctgagcca gtagttttct tgatgttctc tggcatcttc
     4621 ttggctttcg cgaatgaatg tcagtaaatg ttcgcatttg atctgcacat cttctttatt
     4681 ttctgataaa agaaattctg ccatttgtct caaaatattt cgaaattctt tttcgatctg
     4741 tatttggttt tccttcagct gcttcgtacg atctggcatg taaacatttg caagcaaagc
     4801 aaatactaat ccaatcgcca ttaatcccgc ttcattgccg atcagctgcc aagacatgtt
     4861 ttcctccact aaataatgag tgaccaaaac cgaatttact actatccctt cggttaattt
     4921 gaagcgagag gcaagcggga tgaacaggaa taaaaaaaga ctgaagccaa aaatagtata
     4981 cccaacagca aaaaaactga acagtgctgt tgccgtagct aatataaagg ctgcgattcg
     5041 attcatgccc gtacgcaatg tagagcgctt ggtatttcct acacttaata atgcaatgat
     5101 ccctgcagct ggccaataca acagcgataa gctacccgct gtgaccatcg aaaagaagac
     5161 actgatcact gttttgactg ttcgcattcc tatacgcatt tacttccccc ttttttattt
     5221 taattatact tgaataatga aaaactagct atcagacaaa agtttaggta tgatagtaaa
     5281 aagaccaaaa aggagaatct ctatgcaatt tggccgtttt cgactaggta tgcgtacgat
     5341 caaaagtgcc ttagctgttt ttttatgtat cttgtttttt catttgactc aacgaggaat
     5401 cccgatgatt gctgcattat ctgctgtttt ttctttgcgt caggacttga ctacaaccgt
     5461 ttcttttgga aagtctagga tcatcggcaa tacattaggt ggtacattag ctatcattta
     5521 tttttttgtc aaaagctatt ttcacaatga ttttttggta gaattgatct tgcttcctat
     5581 tctagttatt attgtgatcg tcgtgtcaga tggtattaac aataattccg gtatcatttc
     5641 cgcaatagct acgttgttgc tgattgcgtt gagcatccct caaggcgaat ccgctttgta
     5701 tgctatccaa cgtgtactag atacttttat agggacattc attgcaatcg gtatcaattt
     5761 ctttcttcgt cctcctgaaa cagaaaagaa ggaagaacta tcagaagatt tggtcgaact
     5821 ccaaaagaaa gaacgattgc taagaacaaa tttacaagag atagaagcta agatccaaaa
     5881 acaaaagaaa aatagcgagg actaattttc ttttgcaata aaataaaaaa atggagagaa
     5941 gaactttaaa aagtcttcct ccatttttga ttgaacggtg aagtgtacta ctttattctt
     6001 ccttcatcga gttttgttct gcctcttttg gcgcttcata catatttact tggctagtag
     6061 tcccacgtgt cgacaaattc tctacgattt ccttcaagtc aagtcctgtt gtcgctttta
     6121 gtgtttcttg agtagaagaa agcaaatttg tggcataatt tgtcactcgg ttcgctccac
     6181 tggtttctcc tgaactgctt cctgtatcga caacagagat tttttcgata tttcctagcg
     6241 gctgtgctgc ttcgcgcatc aattgcggta gcatatcgat aaccatactc aagacagctg
     6301 cttcgccata ttccttgaag gcattggcga tcttttgttt tgcttcggct tctgcttttc
     6361 cttttgctag tgctgcttcg gcttctgctt gtccagctaa acgtgtctta ttggcttccg
     6421 cttctgccag tgcttccaca cggaatcgtt ctgcttcggc ttcagtcact tctttgactt
     6481 tttgagcttg tgcttcttgt tctttagcgt accgatcggc atctgctttt ttcttcactt
     6541 ccgaatcata ttgcttttca cgacgtgtga tttctttttc ttccagttcg atttgttttt
     6601 gacgttctac gattttaatc tgcatttcct gttcgattac ttgttgttgt gctcgggcac
     6661 tttctaaatt gtaggcttgg tctgcttttg ctttagcaac atcttgttct tgtttgtagg
     6721 ttgctaattt cagttctttt tcttttaatg attctgcgat ctctgtttgt cgctgtaatt
     6781 cagctgcttg tgattctttt tctgcttgag cttttttgat acgcgtttct tttagagctt
     6841 ccgcttctgc tatatctgca tctcttttga cttgggcgat tcttggtttc cctaaagagt
     6901 caagataacc gtttttgtct ctgacctctt tgatagtgaa agaaacgatg atcaacccca
     6961 tttttgcaag gtctacagaa gcgacttctt gtacgctttg actgaactta tcgcgatttt
     7021 gatagatttc ttctacagtc atagaaccaa gaatcgagcg caagtgtcct tctaatactt
     7081 ctcttgcttc attttccagt tcttctcttg tcttgcctaa aaactgttct gctgctgtag
     7141 caatctcttc tacagatgaa ccgattttga tgatcgaagt accatcacac atcaccggta
     7201 cgccttgttc cgtatacact tcaggggtgg agacgtccaa tttactcgat aatagactga
     7261 tccgattcga acgttgaaaa actggcaaga cgaatgcgcc acctccacgc acaattttga
     7321 ttttgttatt gctttcatca atgtgcacat ttttgcttcc taaataactt ccactgatga
     7381 tcaacgcttc atccggtttt gctgtctggt acttcgctac aaagacgatc agcaacatca
     7441 gtaaaatcaa agctgcgatt gcgatcgcga taaaaacaac tggtatttcc atttaacaca
     7501 cgctccttta agcttttatc atgctttcat aaggcatgac ataacagatt ctttccttga
     7561 tctcaatgat caatactttc gttccccgct cagctgtctg ttcttggggg ctgtaaaaac
     7621 aagctggacg actaatggaa ccggtaacac ttttcaactg aatttctccc atacctcgta
     7681 cagggatcgg tgtgatcaca acagctactt gtccttccag agttttttca gaagacgaca
     7741 tcgttgattc ggcatgtttc atcggcatca atatatagaa attaagcaaa aataccaaca
     7801 ctatcgatat gcctccacta gcaataaata aaaataaaga tgattgatct gttaaacgct
     7861 cacctagaaa accaaataag cttgtgaaag ttaaccaagg gataataagc atcggatcaa
     7921 gcgggccatc aaaatcgaat acatctccaa aaataatcaa taggactgcc aatcctgcac
     7981 aaatcagcaa tgtataccaa taaatcgtct caagtgcgat tccccctatc atatacacgc
     8041 ctccttccat ttctacgtct atcataccaa tgtttgaaaa ttctgtatag cttcttttac
     8101 cgactcagtg gtgtttttta taggaaggtt gtgtttatcg ttgacaattt gttaaatttt
     8161 tgttttcatg ttaaaatttt cacaataaat tctttcgagg tagcctatgt ttcctttttt
     8221 taagaaaaaa ccattaaagc tccagaacac gatcatcctt gtcgtttgtt tctccacatt
     8281 cgtttctttg attgtttctg ccatattgat caggaatttt attctttcga tgaaccgatc
     8341 tagtgtgtca gataaaatct ctagcattgc taaaatcgtt gccaatgata aactcatcca
     8401 aaaaaagctg cagactggtg acccgacaca tgcgattcaa ggaataacaa atgaaatcat
     8461 gattgcatcg aatttagatt ttatcgttgt catggaccct gaaggcattc gatattctca
     8521 cgcagaccca agttacatcg gtcttccttt ctcaagtcat gaagatgtga aaaaagtgat
     8581 tggaggacaa gaatttttct ctgaaaaaga aggaatgtta gggttcggct tccgctattt
     8641 cgtccctatt tattatgatc aagagattat tggtgcagtt tgtgttggct taacaaacaa
     8701 cacgattgac aatcaagtca aagatggaca aaaaatgatt gttttttcat tgattattgg
     8761 acttagcgta ggcatcatag gcgccgtttt tcttgcccaa aaaatgaaaa aagtactgct
     8821 tggacttgaa ccacaagaaa tagcagaaca gttaagtgaa aaaacgatta tcgaaaatga
     8881 agtagatgaa ggaatcttag ccatctcttc tgataaaact atcctcttga tcaatacacc
     8941 tgcaaaaaac attttctacc aaacagagca gctattggat ttcgaagtag gaaaaaaatc
     9001 agtgataatc tatatgacgt tttgtttaaa gagacgattg agcatcaaac gaccgttcgg
     9061 actcaatcca tgtatttaaa tgggatagaa actatttcta gcgttacacc tctttttagc
     9121 aatgaccgtt ttttaggagc agtcgcaact ttacgaaatc gatcagaagt aatgaaatta
     9181 atggaggagc ttggggggac gaaacaatat ataaactcca tgagagctca aacccatgaa
     9241 ttcatgaata aacttcatgt catttctggt ttgattgatt tgaagaagta tgaagaagtc
     9301 acccatttta tccagcagct aaatcaaaat tataaaaaag agattggcca agtctcaagt
     9361 ctgatcaaag tacctgcaat tgccggtttt ttattaggca aaataaacga agccaaagaa
     9421 caatctgttg aaatgctgtt agaaagcgat tcttttatcc ctaaaatgga gatggatgac
     9481 tccatccatt cacttttgca agtgttaggg aacttacttg ataatgcaaa agaagccgtg
     9541 ctacaaaaac atgaaagaaa cggacaggtc cggattcatt taaactatga cacagaagct
     9601 gaagtattca tcgtgaccgt ctgcgatgac ggtgtcggga tatcagaaga aatctcagaa
     9661 aaaatattta cgctaggttt ttcgacgaag ggaatcaatc gtgggtttgg attgaatctc
     9721 atccattcaa tcgtcaaacg ccacggtgga tttttagaat tttccaaaca aacaaatggc
     9781 ggtacaatcg tctatctaga attaccaaaa aaggaagatc aaaatgatga caaatgtcct
     9841 gataatcgaa gatgatccaa tggttgcaga attgaatcgt acttttgtgg aaagcatccc
     9901 taatatgaac gtaatcgaaa gtttcagaaa tgtagaagaa ggaaaaaaat atttcagaca
     9961 aaaaaacgca gatctgctgc tgcttgatgt atacatgcct ggcgaaagtg gtatccattt
    10021 tttatcttgg cttagacagc aaggaaatga tatcccagtg attttgatca ctgctgcaga
    10081 tgatatcgaa accataaaga atgtgatccg ctacggtgcc ttagattatt tgatcaaacc
    10141 gttcactttg caacgttttc agctggcgat cgaaaagtat cttgctttct ctcagacaat
    10201 ccaaacgact gaaaaagccg atcagaaatt gatcgatcaa gtattcaacc acaccactcc
    10261 cattcacgaa ataccatcat tcatcgattt gcctaaaggt ctgtcaaaac tgactttaaa
    10321 aaaaataagt gcatttattc taaaagaaga tactattttt tcaacaaaag aactggctca
    10381 aaaaagctca ctgtcacgta tatccgtcaa gaaatatttg aattttttag tagaacaaga
    10441 tatattgaac gaaagaatca aatatttgga aatcggccgg ccgctaacct gttatgaatt
    10501 gacaaagaat gggcggaaaa agctcacaga ttactgttga gtcatcgaat tatcccatac
    10561 aaaatagtcc aagtatacag aagtaacaac tgtttacttg gactattttt aaacatttta
    10621 ttgacgaata tccatgtttt cttatcttaa ttgacgggct ttaggaagag atacctcatt
    10681 tttcgttttt ccagtttcta ttatttcacg aaagttctcg aaacttgtcg aaatcatatt
    10741 tgataatgcc tcatctgtat ttgaacctac atgcggggtt acaagaactc tagggtacat
    10801 ttctactaat tgctggatag cagggtctgg aatcgtctcc catggcttga aggatcggaa
    10861 aaaaacctct gtttcatttg caaaaacatc ggttgcaaaa ccagctaaat gattgctttt
    10921 caaagctctt aaaatagctt cattatcttg aagctctcca cgggctgtat taatcaagat
    10981 gctacctttt ttcatttgag ctaaaaatcc ttcattgatc atttgatcgt tttcacctgg
    11041 gatatgaggt acatgaatac tgacaatatc gcttttggct aataactctt caagcgagtg
    11101 gaacgtcaat acttttttag cagcttctgt ctgatgaata tcgtagccga tcactttggc
    11161 tcctagtccc ttaaacaaaa cagcttccgt caaccctatc ctgcctgttc ctatgatccc
    11221 tactgtgcaa tttctgattt cacgactaaa catttgctca tccactcgaa aatctttgaa
    11281 agatgttcgg atcgtcgtat acgcagtatt tctcaaaagc atcatggcaa aagtcaaaga
    11341 taactctgct atcgcatttg gcgaatacga tggtacacgg gctactgtca taccaaaatc
    11401 agctgccgct tgtagatcga tatgattgac tcccacagtc cgtgtaaaaa cataacgaat
    11461 cccatattcc tgcatttttg caagattggt ctcatctgct atacaatttc ccctcaacaa
    11521 tacagcatca tggtcctttg ctgacacgat attgtcatgt gtcaacagtt cttcataaag
    11581 actgagatca taatggtaaa tattcaattt ttcaaaaaaa tctatctcgt tcggacgaac
    11641 accgtaacat gcgattctca tactttcttc ctccatttct tatgaaaata aacctatgcc
    11701 atgaatcatt tgtaatacaa cgatccatac aggtgtcagt accacagcaa aaatagttga
    11761 cagtaaggat gcattggagg ctaaaagtgc ctccctatca aaactgatag cataggctgc
    11821 tgccactgtt gcagctggtg tagccatcat gataacgatt gttgccagtg cttcaaaatt
    11881 gactggtaga atattcgttg ccgtcaaaac tgctagaatc acgatattca atgcaggtac
    11941 aaataccact ttgactagac tgtaatacca tgaagttttt tcagatgcag cttttttgaa
    12001 gcttacttca cctaatgttg cgccgattgc tagccacgcc aatggtgaac ttaaaccagc
    12061 taaataatcc atcggctgaa atagccacgg tgcagtctgg tcaattcgca aaaacgctac
    12121 ttggcttatc tctccttctg cacctgtaac tgacacttgt ggcagatacc cttggaacag
    12181 ccaaatgaaa aggccggcaa aagtagcgat aatgattgga ttgacgaaca tcgctttgat
    12241 atttttagct tccatcttca agccactcat ttttatgtat ccataagaat acaaaaagat
    12301 tcggtagcca atattgaaaa ttgaggaata catcacgccg acaggaccat aaatcgcact
    12361 gacgataggc gttccgaaaa aggtcgtaga accaaagatc gtcaggacac gcaacgttgt
    12421 ttgcttatcc cctttatagc ttaagaaaaa aggttttgaa actagaataa gaataatata
    12481 aataactaat ccccaaatca agacattgat tccttgcatc agtaattctg tattgatatc
    12541 ttgcatgaaa gctcgaaatg ccagtgctgg caaagccact gttaagacga ctttcgatag
    12601 aatttttcca acagattcag aaaaaatccc cttttttcta caaacaaacc ctaataatat
    12661 aataaaaact gttgaagtga ttgcactgac aatcgacata tctgtcaaag tcgatcgaaa
    12721 aatgtcaaat aagttcatct ttctcctcct aattatgttc atttattc
//
LOCUS       DAHHZJ010000073        12765 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.074, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000073 DAHHZJ010000000
VERSION     DAHHZJ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.074"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            66..3593
                     /locus_tag="NBL02_001635"
     CDS             66..3593
                     /locus_tag="NBL02_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HCD2572319.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3590..7312
                     /gene="addA"
                     /locus_tag="NBL02_001636"
     CDS             3590..7312
                     /gene="addA"
                     /locus_tag="NBL02_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HCD2572320.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7381..7722
                     /locus_tag="NBL02_001637"
     CDS             7381..7722
                     /locus_tag="NBL02_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD2572321.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8031..9074
                     /gene="pheS"
                     /locus_tag="NBL02_001638"
     CDS             8031..9074
                     /gene="pheS"
                     /locus_tag="NBL02_001638"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCD2572322.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIVRKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9079..11499
                     /locus_tag="NBL02_001639"
     CDS             9079..11499
                     /locus_tag="NBL02_001639"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCD2572323.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11669..12679)
                     /gene="trpS"
                     /locus_tag="NBL02_001640"
     CDS             complement(11669..12679)
                     /gene="trpS"
                     /locus_tag="NBL02_001640"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCD2572324.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
BASE COUNT         4312 a         2233 c         2713 g         3507 t
ORIGIN      
        1 acaaatgttc agtatttttt gatataataa aggagcaccg tatcatgtgt tgaaggagga
       61 aaagcatgtc tttacaattt attcttggaa acgggaaaaa agatcaccgt caagtcttga
      121 tcgacgaagc atccgcttgg ctcgaccagg atgcaagtaa ccaagtattt ttccttgttc
      181 ccaactacag caaattcgaa caggaacaag aaattttagc ggagatgcga agaagaagcg
      241 gaaaaaaaac tttcagcaca acaaacgttc aagtatttag tttttatcgt ctggcttggt
      301 atttgctgca acagacaact cttcttacag gaaatgaatt atcagaatca ggatctgcaa
      361 tgatcttgcg acagatttta gaaaaaaatg aggaagaatt aacaatcttt cgcggagaaa
      421 tcaataaaag agggtttatt cgtcaattac aagaactcta cagtgaattg cgtacaggaa
      481 atattcaacc agaagacctg atgcttttat ttggtgattc acctaatgca gaagaccagc
      541 aacttaaaat gaaagacttg aagctgattt tttcagctta tgacagcgaa ttgactcaac
      601 gtgcactaac aaatgaagat gcactatcta tactagcaaa ttatatgcag acacaagatt
      661 tcaacaacgt caaatttatt gtcagtggtt tttctcgaat caatggtcaa gagtatcaat
      721 tactgcaggc aatgatggaa aaggggcaat tggttgtcga tttattgctg gatcgtcctt
      781 atacagcgga tcttccagaa gtacttacgc tctttcatga gactggaaag ctttatttcc
      841 gtctttttca aagtgcaaga gaacggaaag tccctatatt agtggaccga tttgcaccag
      901 agcagactcg tccgacagag cttcaactgc tgcaaacaat gtgggaagag acatctagcc
      961 aaaagaaagc ggcagtacac aaaatagggg gacaagagca tctccatttg tgggaagctg
     1021 aaaatccaac tgaagaagtc cgacaattag ctgttgaaat cagacgacta gtcagtgaag
     1081 aaaactaccg ttatcgtgat atccaagtgc tgacaaaaaa tatgtcttta tatggaaata
     1141 tccttcggcc gatctttcaa gaaatggcaa ttccttttta tatcgatgaa gaacaaatga
     1201 tggaaaatca tccgctgatc gaatggatca acagtctgtt cgctcttgac cgctattctt
     1261 atcggctaag tgatattatg cgttttttca aaactgaatt atttctgtcg acggaagcag
     1321 aaaccgaact tgatgaatgg aaacaagagc gagatgaatt caggaaaaaa ctggacatca
     1381 ctgaaaatgt agcacttgct tacaattatc aaggaactta ttggactcgt gaaaaagact
     1441 ggcagtttgt ggcgtatgat tttgaagcag aacagattga agacgtacag tatttggaag
     1501 aacagtcaaa tacgattaga cgattatttc agaaagctgt accagtattt ttgaaaaaaa
     1561 taaagaaaag caaaacagga caagaggccg caaatgtttt ttatcaattt ttgatccaaa
     1621 gtggcgtaca gcaacaatta ttgttttggc gtaatcagga agtagacaga ggaaacttag
     1681 aagcggcaag aaaccatgag cagacatggg gagcgctgat tgatctgttg gatgaatatg
     1741 tagtaattta tggtgaatcg gcttttgact ggtcactgtt ccaagaaatc attacaagtg
     1801 gtctagaaaa tctaagttat ggtaaaatcc ctaccgcaat cgaccaagtg cgaatcaatc
     1861 gtttggaatt agttcgacca aatcaagctg ccgtcacttt tgcattaggg ttgaatgatc
     1921 aagtgtttcc agaccgaaaa gagacaaaag gattgttgtc cagtgaagaa cgagaagtat
     1981 taaatcaggc cttgccagat ggacagtttt tattcgaccc atcaaaggaa agtatctctt
     2041 ttgagccttt ccaagcctat cttgtttttc tttccggaac agatcgatta tatcttagtt
     2101 atgcacaaag ctatgacaca gatggttcgc tgaagatgtc tccttatttg cgtagaatca
     2161 cagaatattt atctgttccc gtcgagagaa aagagcattt gacgatcgaa agcgacccta
     2221 attgttatgt agggacttac agaagcggaa tcaatacagt caataggatt tatcgattag
     2281 ctttagatga aaaaaaacct gtaccaaagc actggcaaaa gctcaaagaa ttagtactga
     2341 attcgtcatg gcgaaatttt gctttacaga tttttgaaag tcaggaacat ttaaatgttc
     2401 ctgtttcgat gccagaaaaa atggcggaag agctttatgg aaaagagatt tatacttcaa
     2461 tttcacgtat ggagaatttt tataattgtg aatacaaata cttcatgcaa tttggtttga
     2521 aattgaaaga acgaactgtt tatggtctaa ctccagcagc aactggtgat ttttatcatg
     2581 aatcattgga tcgtttcttc aagctactgt tttcaaacca attatcctta gtgaatatgt
     2641 ccgatgcgca gcgtagagac tttactgaaa aagtgctgca ggaagtattc ggagaacttc
     2701 ggtttgagat attggatagt tctgcaagaa tgaactatat ccgctaccag ttaggacaga
     2761 cgattcaaaa agtgacatgg gctttgcaaa aacagagcaa aaatacaggc atgcatcctc
     2821 ttcagacaga gattctattt ggtcagattg ctggagcaaa aggtatcaca ggtctggaat
     2881 taccgttgag taatggtgga aaactccatg ttcgagggaa aatcgatcga atcgatgtag
     2941 cttctgaaca agaagatact tggctaagcg tcgttgatta caaatctagc ggacgttcat
     3001 tcgatgtcac agaagcttat tatggcatgg ctatgcaatt attgacgtat ctggacgtag
     3061 cattgatgga tgcggtacgt ttgactggaa aggcggctgt taaaccagca ggatcatatt
     3121 atttacatgt tcataatcct gtattgacgg agacagatga tgtagagaag aatacattga
     3181 aaaaatttag ttatgatggc gtttttgtcg atgatcccgc tcttttagaa gtactagatc
     3241 attcactgga agcaaaagaa caatcactga tctatccagt gaaaaaaaat gcgaaagatg
     3301 aataccaaaa aggtagtttc agtaaagaca aatttttcac ggaagaagaa ctgcaactat
     3361 tcatggcgca taatcgggaa aatatgcggt tagctggaga tgcgattact tccggagaaa
     3421 tcaaactaaa tccagcttat aaagataaac agcggattgc ctgcgaattt tgtccattca
     3481 gaagcatctg tacgtttgat gtgatgctga aagaaaataa ttaccaccgt ctggagaaaa
     3541 tagataagga agaagcactg cgtagaatat tgaaaaggag tgaagaagag tgaatcaaat
     3601 tcctttaaaa ccgcaaaatg aacgattcac agatgatcag tggcaagcga tttttgatca
     3661 aggagataat ctattagtct ctgcgtctgc cggatcaggt aaaactacag tgcttgttcg
     3721 aagagtgatt gaaaaactaa aaatgggttt tgatatcgat gaattattga ttgtgacatt
     3781 tactgaagct gccgcaagag aaatgaaaga aagaattcaa gaggcattac aagaatccgt
     3841 caatagcgaa agtgaccctg tacgtagaca gcattttact aagcagttag ttttattgcc
     3901 cacagcgaat atctcaacct tacatgcttt ttgcctgaca gttattcgac gttattatta
     3961 cttaattgat attgatccag tctttcgcat gttaacagat gaaacagaaa cgatcttgat
     4021 gaaggaggat gtgtgggacg agctgagaga agcactttat gcagaaaatg atgaacgatt
     4081 tttccaatta acaatgaatt tttctaatga tcgttcagat gacggcttga cgaatctggt
     4141 gttttctctt tatgagtttg cccgtgccaa ccctgacccc caaaaatggc tagaacagtt
     4201 aagcgataat tatcgtttgc ctgaggggct agcgaaatct cgattatatc aaaaacaaat
     4261 ccgtccttta gttttggcag atatatacca atgtgtacag ttatatgaac agatgactca
     4321 gttagcacaa ggtgaaggat tagaaaaaat gcatgagcaa gtagctggag aacaacagca
     4381 gatcaagaat atctatgaag cgttctcgca agatcggtta gaagaagctt atgctggttt
     4441 ggaacaacta actttttcaa cttttaagag cagtcggaaa gcagaattga aagaaatttc
     4501 caatgaagtc aagggcatga gggataaagc aaaaaaactg attcagcaga taagtaaaag
     4561 ttattttcct gtttccccaa gccaaatgga agaattaacg gacaaagcac taccgttagt
     4621 cgaagaaatg acaaaagtca cgcaaagctt catggacggt ttcagtatgc gtaaacggga
     4681 aaaaggtgtg ctggatttca atgatctgga acatttagcg ttgcagattc tcacagaaaa
     4741 aacgaaagat gtatggctgc caagtgaagc ttcaaaacat tatcgaaaaa aattcaaaga
     4801 agtaatggta gatgaatatc aagacgtgaa ccaattacaa gaagccattt tatactggct
     4861 aagagaaccc gatgatacaa aaggaaatat gttcatggtt ggggatgtca aacaatccat
     4921 ctattctttc cgtttagcag atcctagttt atttatcggg aaatacgaga atttctcaaa
     4981 gaaagaaggc ggacgtcgaa tcgttttggc tgagaacttc cgctctagaa aagaagtcct
     5041 ttcttttacg aatcttattt ttgaacaact aatggatcca gcagtgggtc aaatcaatta
     5101 tgacgaagca gccaaactga tccaaggatt ttcagacttt ccagagaatg aacagtttga
     5161 gccggaaatc atgatctatg aaaaagaaca ggaagaatca gagattgaga ttccgacgga
     5221 tgatatactg gaagacaaaa cagaaggcga actttttatg acaggactaa aaattcgtca
     5281 attaattgat tcctccttta tgatttatga taaaaaatca aaaaaaagtc gtccgataga
     5341 gtacaaagac attgttctct tgacacctac aaagaagaac aacttaacga ttttagagat
     5401 tttcaaaaca ttggatattc cattagagat gaatgatgct caaaactatt ttcaagcgac
     5461 agaaattcgc acgatgatct cgctgctaca attaatcgat aatccctatc aagatattcc
     5521 gcttgcggct gtattgcgct caccgatcgt gggtctgatt gaaccagaat tagccagcat
     5581 tcgattagct aaccgtgcac atacttacta tgatgctgtg cttgcttatc aagcatcaaa
     5641 cgaagatgaa ttagcagcta agctggaaca ttttggcaag caactagagc attggcgaga
     5701 acttgctcgc cgttcttcga tcactgactt gttatgggat atctattatg agacgggata
     5761 tctagagtat gtggtcggtc tgccagcagg ggcacagaga caagccaatc tttatgcatt
     5821 ggttgatcgt gccaaagctt acgagcaaag ttctttcaga ggattatacc agttcgtgcg
     5881 tttcattgaa aaaatgcaag aaaaagataa agacttagca gaaccagtca tttcaataga
     5941 agataatgct gttcgcgtta tgacgatcca tgcaagtaaa ggcttggaat tcccagttgt
     6001 atttttgtta gatatgacaa aggagttcaa cttacaagac ttgcgcaatc ggtatgcttt
     6061 tgaagaaaaa cttggagcag gaattcgcta tatggatcct gaaacacgag tgttatatga
     6121 tacgttaccg tttcaagcca tcaaattggc aaagcaaaac aaattgttgt cggaagaaat
     6181 gcgtaaattg tatgtaggat tgactcgggc agaacaaaag cttttcattg tcggttccta
     6241 taaaaataaa gagcaaatga tccaaacgtg gtctgaagca gctgatcatg aggagttagt
     6301 ttttgatcct gcgctacgtt tgaagggcag aagcagcttg atgaattgga ttggttacgg
     6361 attgatccgt catcctgaaa tgcagaaata cttagaggaa gaaatttcga cttctcttct
     6421 tcaacattca aatgcacagt tttctatttc atggatgaat cagcaatcga tcattgaaca
     6481 aagacagttg cttgctgaaa aagaactggt taacttagat cagcagatga aagaagatga
     6541 aactttatta gcagattcgt tacaaaaacg tctggcatat gaatatcctt atcaagcttc
     6601 ttcacaaacc acaagttatc aatctgtatc tgaaatcaaa cgattattcg aagatccaga
     6661 tgatacacaa gaatcacgtt tgactctgga aagcagtcag aacaaagcag catcccgtca
     6721 atttcgttat acgcaagaac aactagcaga accaaaattt ttgcaaaaag atcggcaagt
     6781 gtcagcagct gctgttggaa ctgctacgca tgctttgtta caattactgc cattagagat
     6841 gccaactaca gagagtatcc atcaaaaatt acaggaattg gtaaggaaac ggttagtaga
     6901 cgaaaaagtc gcgaaaaaag tagatgtttc ttctattata tggttctttc aaacagaact
     6961 tggtcaacag ctgattgcca acaaagaaaa cgtaaaacga gaacaacctt tttccatgct
     7021 gcttcctgct gacgaagtat ttcaagacta tccaaatcaa gaagatgaac tattaatcca
     7081 cggaatcgtc gatggatacc tagaagaaaa agatcatctc aatatctatg attttaaaac
     7141 agattttatt ttacatccgg atgatccagc agaaatagat gcaatcgtcc aaaaatatca
     7201 aggacagcta agactttacc aacaagcaat gtcagaagct ttgaataagc cagtagaaaa
     7261 tgttttccta attttgcttc gagtgaaaca aattataaac attaacaaat aattataatt
     7321 gagcaaactt gacttatgtt ttgtaaggat tatgctatac tctgggaaga ggtgagtatg
     7381 atggagctaa cacagacaga attcgtttta tgccctaaat ttgaaaaaac attctctatt
     7441 ttaggaaaaa aatggaacgg attgattatc gatgtactac tcgaaaacgg tccgcaacgc
     7501 tttgttgagc ttgcggcaaa aatcccgatg gtcagtgatc gagtattagt cgaacgcttg
     7561 aaggaattag aaaaagaagg aatagtcact cgaaactttg cctgtagcga aaagagccgt
     7621 tcagaatatc tcttgactga aaaaggagca gacttgcaaa aagcgatgcg tcaaatacaa
     7681 gtttggagcg aaaaatggat cacagaggaa gaatgcggtt gattttgtaa aaaaaatctt
     7741 gtaaactata gtccatacga ataagtaaag aacgatgacg aaaaagagta gtttggctaa
     7801 accagttcag ggagatctgt cgcagactga aagcagatcc tgtgtttgaa aaatgaagtt
     7861 cactttccga gttcgtctga taaaatcaga ccggcaattg tcgttaacga agtaagagtg
     7921 cggtgtaagt catttatccg caaattagga tggtaacgcg aagacctcgt tccttaacag
     7981 gaatgaggtc tttttgtttt gtatttggaa tttaaaggag gaacaagcac atgtctctac
     8041 aagcacaatt agagagcttg aaagaagcaa cgctcgtcaa aatcacagcg gcacaagatt
     8101 taaatgaatt aaatcaaata cgtgttgaaa cgttaggaaa aaaaggaccg atcacagaag
     8161 tattacgtgg gatgaaagat ctaagtccag aagagcgacc aaaagtcgga agtttcgcaa
     8221 atgagatccg agatcttttg acagaagaga tcgtgcgaaa aaaacaagta ttagaagaag
     8281 aagcgttaaa tgcagctttg gcaaaagaaa caatagacgt gacactacct ggtagaaaag
     8341 taaaacatgg tacacgtcat atcgtgacac aaatcatgga agaaattgaa gatatttttg
     8401 tgggtatggg ctatcagatc gtagaaggat atgaagtaga atctgatcat tacaatttcg
     8461 aacggatgaa tctgccaaaa gatcatccag caagggacat gcaagatact ttctatattt
     8521 cagacgagat attgattcgg acacatactt caccagtcca agcacggaca atggaaaaac
     8581 atgatttctc taagggtgct ctacgaatga tctcgccagg gaaagtcttc cgcagagata
     8641 cagatgatgc gacccacagt catcagttcc atcaaattga agggctagtt gttgataaaa
     8701 acatcacaat gggcgacctt aaaggaacat tagaagtcgt aatgaaaaaa atgtttgggg
     8761 aagatcgtga aattcgtttg cgtccaagtt atttcccatt tacggaacca tcagtagaag
     8821 tagatgtcag ctgtttcaaa tgtggtggtg ccggttgtaa cgtatgtaaa tacactggat
     8881 ggatcgaaat tttaggagct ggcatggtac acccgaatgt gttgaagatg tcaggaatcg
     8941 atccagaaga atattcaggc tttgctttcg gccttggacc agaccgcgta gctatgttgc
     9001 ggtatggagt gaatgatatc cgtaatttct atcaaaatga cttacgtttc ttaagtcagt
     9061 tcaaggggga gtaagaaaat gttagtttca tataaatggc tcaatcgtta tgtagattta
     9121 tcgaatgtaa caccaaaaga attagcggat aagatgtctg tgacaggaat cgaagtagag
     9181 ggagtaacgg ttccagaaga aggcttgaaa aaaatcgttg tcggtgaagt caaggaatgc
     9241 gtaccacatc cagattctga tcacctttct atttgtcaag tcgatattgg cgaagacgaa
     9301 ctgtctcaaa tcgtttgtgg agcgcctaat gtgaaggttg gaatcaaagt gattgttgca
     9361 cttcctaact cacgtatcgc tggtaatgta aaaatcaaaa aaggaaaaat gcgtggccaa
     9421 gtatcaaatg gcatgatttg ctcattacaa gaaattggct attcagatag tgttataccg
     9481 aaagaatacg ctgaaggtat ctattatatg ccacaagaag ctgtaaatgg cgatccagtt
     9541 tttccttatt tggatatgga tgattcgatt attgaattat ctatcacacc gaatcgtgcg
     9601 gatgcattgt caatgcgtgg cgtagcttat gaagttgggg caatttatcg tcagactcct
     9661 acattcgatg acgaaaagct ggtagaatcc tcacgtcctg ctagcgataa aattcaagtg
     9721 gaagttgaag ataaagaagc agttcctgct tatcaaatca gaatcattga aggagtaaga
     9781 atccagccaa gtcctcaatg gctgcaaaat cttttgatga acgaaggcat tcgtccaatc
     9841 aacaatgtag tagatgtgac gaactatatt ttgcttttat tcggacaacc actacatgca
     9901 tttgactacg acaaattgaa atcaaaagag atctttgtcc gccgtgcaaa agaaaatgaa
     9961 acattagtaa cactagatgg agaatctcgg gaattgacaa ctgacaatct cgtcattact
    10021 aacggcaaag aaccagttgc tttagctggt gtcatgggag ggttagactc agagatcacg
    10081 aatgaaacaa caacagttgc actagaatct gctttatttg atccaatttc gattcgtcgt
    10141 acagcaaaac aatttaattt gcgtagtgaa tcctctgcac gttttgaaaa aggaatcaac
    10201 catgcgactg ttggagaagc cagtgaggtt gctgcagcaa tgatcgtttc tcttgcgggt
    10261 ggggaagttc tacaaggcgc ggtcaaaggc tcagagatca aagcagaaga cgtatcagtg
    10321 aagatttcac ttgaacgcat caatcaatat ttaggaacat ctcttgtcgt taaagaagta
    10381 aatgagattt ttgaagcact tggatttagc tatgaagaaa aggagtatgt ttattccgta
    10441 gtagtaccac caagacgctg ggatatccaa atcgaagcag atattatcga agaagttgct
    10501 cgtatttatg gatacgacca gttgccatct acacttccaa gtggagaaac ggttgctgga
    10561 agcttgtcaa aagaacaaga aactactcgt aaaattcgta caatctttga aggaagcgga
    10621 ttgagcgaag caatcagtta tgcattgaca acagaagaaa aatcaagaca gtttgttata
    10681 aatgaaagta atattacacg tttggactgg ccaatgagcg aagaacgttc tgtattacga
    10741 atgaatttga tcagcggact attagatgat atcagttata acactgcccg aaaaaataca
    10801 gagattgctt tatatgaaat tggtcgggtg ttcttccaag aaaatgatcc acgtacacat
    10861 ctgccgcaag aagtaaaaca tgcagcaatc gctatttctg gtatttggga agaaaaagat
    10921 tggcagacga aagctcaaaa tgtagacttt ttcaccatca agggtctttt agaaaactta
    10981 tttgaacaac taggcatcac tgaagatgtc cattatcaaa cagtgacaga aatgaaggaa
    11041 atgcatcctg gaagaactgc tgcgatttac cttggcgaaa aatttattgg ttttgtcggt
    11101 caagtgcatc caacgattac aaaaagttat gatattccag aaacatatgt tgctgagttt
    11161 gatttatctg ctatagtgga agctgctcaa aaagggatcg ttttcgaagc agtgacaaaa
    11221 ttcccagcag tcagccggga catcgctttg cttgtaaaag aaactgtgac aaatcaggaa
    11281 ttaacagaag ttatccgttc tgctgccgga agatttttaa cagatatcca attatttgat
    11341 gtctatcaag gagaaaacat tgaaaaagga cataaatcca tggcgtatag cctgacattc
    11401 gtcaacccag aagcaacatt gacagatgaa gaaatcaata aagcaatgga aaaagtcgaa
    11461 aaagcgttgg tagaaaacct agaggcaagt attcgctaag aaggaaaata tagtaagtaa
    11521 cgatcaagta gcttttgaag accatacatt tgaaaaaaag atgtgacaca acttcgtcac
    11581 atctcttttt ttgattttaa aaaaggaata gcacagagac gagataaaaa aacaggcctt
    11641 tacagcctgc ttttttagag taaatgaatt aatggaagta attgattccc attgctgatt
    11701 ttacttcaga aagtgtttgt gcagctactt tagcagcttc ttcactacct ttacgtaaga
    11761 tttccataac agctgaagga tctttttcca attcttctcg gcgcgcacgg atcggtgcaa
    11821 attcagcttc caatacatca atgaggtatc gtttgatctt tacatcacca agtccgccgc
    11881 ggcggtaatg agctttcatt tcttcaattg cttctttatc tgttccgaat acatccaagt
    11941 aggtgaagac catattccct tctacttgtc caggatcttg tacatggatg tggtttggat
    12001 cagtatacat actcatgact tttttctgta agacatcggc aggatcggag atataaatac
    12061 cgtttcctaa cgatttactc attttgccat ttccatcgat tccaggtaat ctacccattc
    12121 cttttggtgg gaatacgcct tttggttcca ccaagacttc accatatgta tgattgaagc
    12181 tttgtacgat ttcctgtgtc tgctctagca ttggtttttg atcctcacca acaggtacaa
    12241 gattcgcttt gaatgcagtg atatctgctg cttgagaaac aggatagata aagaatcctg
    12301 taggcacgct ttcaccgaat tttttttgtt cgatttctgt tttgacagtc ggattgcgcc
    12361 gaacgcgtcc tacgctgact aagttcaaat aatacatggt taattcagct aattccggaa
    12421 tttgtgattg gataaataaa gtagattttg caggatctaa tcccacggct aaatagtcta
    12481 aagcaacttg caagacattt gatgaaacct tctcaggatt tttagcatta tctgtcagtg
    12541 cttgcatgtc agcaatcatg acaaacagtt ggttgtcttc atctgcctgc atagccactc
    12601 gggtttttag agaaccgacg taatgcccta aatgcagctt cccagttggt cgatctcccg
    12661 ttaaaataat atttttcaaa aaagacacct ctttacgatt tgtaagtctg agcacttaat
    12721 atcttaccgt ttttcctgaa aagaaacaac agttcctaag gaaaa
//
LOCUS       DAHHZJ010000074        12736 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.075, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000074 DAHHZJ010000000
VERSION     DAHHZJ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.075"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="NBL02_001641"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="NBL02_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCD2572325.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="NBL02_001642"
     CDS             complement(322..891)
                     /locus_tag="NBL02_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HCD2572326.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="NBL02_001643"
     CDS             1077..1979
                     /locus_tag="NBL02_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCD2572327.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="NBL02_001644"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="NBL02_001644"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCD2572328.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="NBL02_001645"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="NBL02_001645"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCD2572329.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="NBL02_001646"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="NBL02_001646"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCD2572330.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="NBL02_001647"
     CDS             8547..8732
                     /locus_tag="NBL02_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HCD2572331.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="NBL02_001648"
     CDS             complement(8794..9990)
                     /locus_tag="NBL02_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HCD2572332.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="NBL02_001649"
     CDS             complement(10160..11596)
                     /locus_tag="NBL02_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HCD2572333.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="NBL02_001650"
     CDS             11889..12698
                     /locus_tag="NBL02_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCD2572334.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2673 c         2250 g         4010 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gacaat
//
LOCUS       DAHHZJ010000075        12717 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.076, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000075 DAHHZJ010000000
VERSION     DAHHZJ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.076"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            29..877
                     /locus_tag="NBL02_001651"
     CDS             29..877
                     /locus_tag="NBL02_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCD2572335.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1523..1729
                     /locus_tag="NBL02_001652"
     CDS             1523..1729
                     /locus_tag="NBL02_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HCD2572336.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(1931..2932)
                     /locus_tag="NBL02_001653"
     CDS             complement(1931..2932)
                     /locus_tag="NBL02_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD2572337.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHFDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(2937..4850)
                     /locus_tag="NBL02_001654"
     CDS             complement(2937..4850)
                     /locus_tag="NBL02_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HCD2572338.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(5018..5524)
                     /locus_tag="NBL02_001655"
     CDS             complement(5018..5524)
                     /locus_tag="NBL02_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCD2572339.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            5684..6124
                     /locus_tag="NBL02_001656"
     CDS             5684..6124
                     /locus_tag="NBL02_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HCD2572340.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(6150..7307)
                     /locus_tag="NBL02_001657"
     CDS             complement(6150..7307)
                     /locus_tag="NBL02_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD2572341.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(7310..7666)
                     /locus_tag="NBL02_001658"
     CDS             complement(7310..7666)
                     /locus_tag="NBL02_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572342.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(7964..8938)
                     /gene="sgrA"
                     /locus_tag="NBL02_001659"
     CDS             complement(7964..8938)
                     /gene="sgrA"
                     /locus_tag="NBL02_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HCD2572343.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(9134..9973)
                     /locus_tag="NBL02_001660"
     CDS             complement(9134..9973)
                     /locus_tag="NBL02_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCD2572344.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNRTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            10158..11045
                     /locus_tag="NBL02_001661"
     CDS             10158..11045
                     /locus_tag="NBL02_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCD2572345.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEGAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(11639..12334)
                     /locus_tag="NBL02_001662"
     CDS             complement(11639..12334)
                     /locus_tag="NBL02_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCD2572346.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(12318..12716)
                     /locus_tag="NBL02_001663"
     CDS             complement(12318..12716)
                     /locus_tag="NBL02_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCD2572347.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3839 a         2500 c         1926 g         4452 t
ORIGIN      
        1 aatcttatta tgaaaatgag gaattctcat gagcgtctcc ataagactac aaatcaaaac
       61 aatgtacaac gacttcagcc caaaagaaca ggctatcgcc gattatattt tagaaaatcc
      121 aagcaaagta agtcatagtc ctattagcaa tttagcaagt gaactgggca ttgctgactc
      181 tacttttttt caatttacaa aaaaacttgg ttataatgga tttaaagatt ttaaaatggc
      241 catgttgatc caagaaaatg atctatctgc tatttctatc cacgaaaata ttcagaaaac
      301 agatactgag ctcactatgg cgcaaaaggt gttcgagtct agtatttcaa ctttaaatga
      361 cacaaaaaag ttgttacaac aaaaggatct aaaaaaagcg gctactatta tcaatgaatc
      421 taaacgtgtc tattttttcg gtgttggcgg ttcagagatt gtagcaactg acgcttatca
      481 taaatttttg cgttccccta tcgcaacttc tcacagcacg gattaccata ttcaattgat
      541 ggaagcttct ttattgacgg aaaaagactg tgctgttttg atttctcaca cagggcaatc
      601 aaaagaaaca atccatattg ctgaaaccgt aagaaaaact ggagcaaaaa caatcgttat
      661 tactagccaa gctaattctt cccttgctaa acttggcgat gtcgttttta tttctatttc
      721 tgaagaaaca gaattccgat ctgaagcttt agcttcccgt atttctcaac taagcatttt
      781 ggactctcta tatgtgattt tgatgtttta caacgaaaaa gaatcacgtc gttctatcgc
      841 taaagttcga catactattt ctgaaatcaa agaatagcac cataacagaa aaagcgaaaa
      901 atccataaaa caaaaaaagc cactaccaaa tatttggtag tggctttttt ggttctgcaa
      961 gggtggtaca gaacatcaat agtcggtggg ttagaacgta tgtcatcgca tctttggaga
     1021 aggttgtatt ggtattattc gagttacttg ttagatgcga taacaaagtt agtatacagt
     1081 agtaaaaaat ttatcttttc ttttttttat tatttttgtt tcttattttc taaaaaatta
     1141 aaaaaaattt tggtgaatta agtatttcct tgataacact ggcttttggc ctcattttag
     1201 gttatcattt gtaattttat ttcacagttg atatgattgt tatgtaaaat aaaaaagttt
     1261 aggatacatc gaatttttgg ggaaattctt ttgggggtat cctaaatctt ttctattata
     1321 ttcccatatt ttctattatt ggtctagacg tatgatggta aactttgtac ctatctgatt
     1381 gctgaatcat taattgctta tcattagcca tctttgtttc aagttcgcta gatgatcatt
     1441 ttattcacat cctaacaaac ttgtcacatt tatagattct taatctattt atcatacaat
     1501 tataaaaaag gaggtgctgc ttatgaaagc tatccatatt tctttgcttt ttttcgctag
     1561 tgcattgatt ttatttgcta ttcatctttg ttcccagcct tatatcaata acaacggaat
     1621 attagtggaa ccagcattct tttgcttacc actcggcttt ttgtcccttc ttgcctccgc
     1681 aggcttcgga tttgttgctt tttttaaacg atcaaaacaa caaatctgac ttataaaatt
     1741 tacgtaaaag agatcgtgac aataatcgtg tgatattccc aaatttcgaa taaaaggtgc
     1801 cccttgccat ctctaactcc tcagtcacaa gttacaatat tcataaacca tgaaaagctt
     1861 gtttattgaa attgctcctt gtgacttgga gctaagtgac agattacaac ctcttttgca
     1921 tcataagact ttactccttg aaaaacatta ctaacttttg atatgtttct tgcgcttgtt
     1981 tattgctgaa atcaaactga tattcatggt tcaaaacagc atttttctcc gcataatcta
     2041 cttttgtgac tttcaccccc gctttctcaa atacagaagt tacttcttcc atttgtttcg
     2101 taaacgtatt tgtatttcca tccgttaaga atgtgttttg cgtccaacga tccgcatgtc
     2161 tgaccaaggc taaacttttg accttctctg attgttcaaa gtccttatta tcaaagtaag
     2221 cccatccaca tttttcataa agaaagctag agattttatc acttacttga gtaagtgcag
     2281 aaggatccaa cagaccagac aaagaaataa acttcgtaat ctgcttgttc tccaaagtcg
     2341 gatcaaaatg atcagctttt ttaatttctt cattttgaag tgctaacaaa tattctccac
     2401 taatttgtgc acctgccgaa tttccaccaa tcgaaatatt cgaccaatct gcttgatctt
     2461 ttatctcttt tttagctgct tgaatggctt cgttcaattg gataactggt gtaggatgtt
     2521 ttgcttccgg tgatcgttca taatctatat ttacgatgat ttgctgcgtg tctgaacata
     2581 gatattccaa ataatcacga caatcatttt tatcccctcc tacatatgcg cctccatgca
     2641 tccagaaaag aatcggtaat tttttctttt ccactttcgg tgtgtagata tccatattat
     2701 tatttttgta ctttgaagga taggatattt ctttttttat atcgatttgt tcgatttttt
     2761 gctggaaatc atctggtcgg gcatatactt gttctttttc acttgtgtca aattgttttc
     2821 taacaagcaa gctaatcggc tttggtgtga tttgattaat caaaaataca gtgactaaaa
     2881 taactacgat gataactggt actgcaatca ctattcgctt gaaccatttc attcatttac
     2941 cctctctttc ttcgccaaat ccatgtgata atcgtgctaa cagataaaaa tccacctgaa
     3001 taaatgagaa gcgaatgttt cttttctcca gttttcggca acattctttt ttttgcatct
     3061 gtcttttttt gtactttcac tgtcatctga atcggttgcg tatcatcact tgattgatcc
     3121 tctcgaggtt cattctttcc cttattgtct gcattctctg tcggatcttg cttttttggt
     3181 ggagctactg ggattttttt gtaatagaaa ataatagttg gtatcgtctc attaaaaatc
     3241 ccttcagtcg gtccttcaac atttgctaat tcgtatcctt cgaatgtctt ttgctctgtc
     3301 atataatttt ctccataagc tccgtaaagc tggatttcgt cctggtcttt gagtagactt
     3361 ttattgtttt catccacata ctttacgttt atctgatcat ggaacactac tttcaaaaag
     3421 tccgttacat gtttcgtgta ggcagcatgg tcaacttcct gtgcctttcc atgccctgct
     3481 ccttctacta ataaaagatc tttataccct cctgataact catataattt ttgcgcgtta
     3541 gacacaggta ctacatcatc tgctgttcca tggataatca atttaggcaa tgaatctgac
     3601 attccaatcg taggtagagc agcttttgta tccaaatgca tctcctgatc aaaatagtat
     3661 tgtttcaaaa ggcccataaa taaatcttca tgctcttgat ccccaatgat gtctaactga
     3721 ttttttatgg catccggtag tgcgggggct actgtctggc tgtacgtaaa acgtagttgt
     3781 tcatcaattg atgcaaatcc gcaattttca ataattcctt tgacattgac aggaatatct
     3841 ttcgctaata cagacatcat cgtagctgct cccatcgaac caccataaag taaaatcttt
     3901 tgagatggcc gctctcgaac ttcttgattt atccagtaaa ggacgtcatc tgactcatat
     3961 tgtccatagg tcacataatc gcctccgcta ttaccagtcg ctctggaatc aacaaataat
     4021 aaattatacc cggccttata aaaatcattg tattcttctg taacgatccc gttattccaa
     4081 ttccctcgaa atccgccatg gatcatgact gttttatctg aaccttgatc gacataatat
     4141 gcatatacat ccatcatttt gccatcttgt ttgtttagta cagtttgatg aagttgtttt
     4201 gtatttggat agaagcctgt gttttctgta ggcgttgtcc catcatctgt acctccaagc
     4261 atcgacatca acacactgtt ttttgcaaaa gcgatatcgt acaatagata agtagcaatc
     4321 agatgtttga taatctcttt atctacacca taatcattaa cagcaagctc agtgactaat
     4381 aactctatta attttgactt gatgtcttct gttggtccat taagtatttc tgtcatctgt
     4441 ttaccgaagt aattcaaata ttctttacca ttgaaatttt cacctttacc gatatcatca
     4501 ttttctgtcc cattctcttt gtcacttact ttagcagaag aggattcgtc gtttgacatt
     4561 tttgtagctg ttgcttcttc tgtttgttgg ttatctgtgg tagcggtcgt ttcttgatct
     4621 gtgctagtaa cagatgtctc aaccgttgtt tcagtatttg tttctgtttg tgaagtactc
     4681 gatttcgttt catttccagc atcagtagtt tctgtggttt ctacctcccg agattccgat
     4741 tcggaagaaa tgctctctcc atatactgtc cattgcggga cgcttgtact tacagcaaat
     4801 aagatgacca acgctccctt taatacttgt tttgttctca agctattcac tcctttttat
     4861 gtttctagga gaattatatg taaacgcaaa cattttttat acaaaaaata aatggtttat
     4921 cccataaaaa tagaacagga ttattttctc attttacgta ataaagcaga atggataaac
     4981 cagtatccat tctgcttttt ttaaacgaag aaacaactta ttgctccaaa aatgtcagaa
     5041 atcgtttatc ccaaattttc tcgtaaaaag cgattgtttc ctttgctttc ataaaaggat
     5101 tatcataagt tgtcgttgct cctctacaca tcataagctg gtatcctaat ccatgagcaa
     5161 acttcacagt cgtatctata caatactctg cttgtgcccc acataattcg atcgaatgac
     5221 agtccaattc agttaataaa acactcaaat tagtcttata aaatgaattc gcatgggttt
     5281 ttctcacaaa ataactgtgc tcaggaatag ataattccga caatagctcc catggttttg
     5341 tttgtggcac taattgagca tcttcgtgtt gaacgaaaat aactggtttc ccaatcgttt
     5401 cgtatgtttt gattctttta ttcactaatg ctgtcactgt ttctaattcg tgtatcggcg
     5461 aagaatcaaa acatacccct ttttgtaaat cgatcactac tattgcgtcg gctgtgtttg
     5521 acatacttgt gccttctttc ttttttcttg atttaatagt atttcttatc tagctagttc
     5581 cattatacgt gacaacattt tcttatttta cacctctaaa tggcactttt cgactatttc
     5641 ttcatgttat tatttcataa atacttagct attggaggaa tgtatgatga ttaaattgta
     5701 tacaggttct ggctgctcgt cttcaagacg cgcaaaacgt tggcttatcg tccatgatat
     5761 accgtttatc gaacagaact taattcgtca accactaacc aaaaatgaat ttatccactt
     5821 actaaaattg acaaacaacg gcgtgaccga aatgctctct actcattcta ttagttataa
     5881 aaatcttgtt cacagaatag atgaactcag tttgagtgaa ctatatattg agattcgtac
     5941 atccccttct ttattgaaaa aaccgattat ctacgaccaa tctgcaaaac ttttgactgg
     6001 atttagcgaa gataaaatca ggatgttcct ttctaaaact aaaagaaagc aagagctcaa
     6061 cgaactcttg ctttctggtc atcttgtcga ttttaaagaa aataatcttt ttgcaatgtc
     6121 ttaattttgg cataaacaat ctgcttttct taattaacga aatccaattt ttccaatatg
     6181 gatctatttt tctcattcct gatttttatg atttcagctt tcagacgcgc ccaatcattt
     6241 tttgtaggtt gtccattcca gtaaaatatc tgtgtattcg tttgatcata tgcctttttg
     6301 tcgataggat aatcgctaat gacaatatca ggtaactgat cagcggatgc actatatttt
     6361 attgggcgtt cagtcataga taatatcctt ttctttgccc ataattcctg tgttttacca
     6421 tactttgatt gaatatagaa tatgacaggt tgttcgtgtt ttataaaaat atccctgact
     6481 aacatacagt attgaaaaat caaccgtctg ttcttttcat aataagtatt aagctctgga
     6541 attctgcgag ctaccccact aaaaaactgt tgcatgattg gatacatcac tggaaatata
     6601 tgctgaaaat ctctgtcttc tgctgatttt ccaaaagcat ccaccttctt ttttgtccca
     6661 aaaacccttg aatacaaatg gatcattgcc aaattgacat atagataatt tttttctgaa
     6721 gttgtaaaac aaatcttgaa gtttttttca aaattttcta caatagacga ttcaatccag
     6781 ccccaacttt cttgtacttt atctggagta tccaacaaat cttcttccaa ataggtgttc
     6841 attacaccaa acataaaata aaggtaagct tgctctgttg gagtgatgtg attcatcgac
     6901 cagaagtttg cttcaaaaaa aggctctagt atgtaatgcg taaactcttc ttttgttatc
     6961 aatggatgca caaatttgtt catctcttct gggacatacg taatcgagtg gccttgtttc
     7021 attcgttgca acgaaattct tagtaggctg atccatttct ttctatctat actacgcgta
     7081 tatggcgcat gtttcgcaaa tttttctaat agttgacgtt tattttttat acttgataat
     7141 atatcagaat gattttcgtc gaatgactgc caatacatca agtgaaaaaa gtatcggata
     7201 cggtattctt ctccttctat cggattacct tttttcaagc tgcaagataa atgatagttt
     7261 ttcaaaatag tattaagttc tcttaatttt ctttgaatcg tacgcaaact tatataattt
     7321 tttgactaag attttctaag gattgataag tacctttgta aagtgaatcc agtaaaatat
     7381 aaccaatcgt tccctctaaa agtatagaat aaactttact cagtaaaaac tgagaagaaa
     7441 actcgacttt tatcatcttg ttttctgaaa gaaaagataa tttatattct ggataccttt
     7501 cgcaaaacga ttctgcttgt tgaatcaccg aattcatctt agccagggtg tagtttagat
     7561 ctaaagcgat atccttcacc acatataatt tctcgtctct ttcataacaa tagttcaaaa
     7621 attgatatac ttgcctttca ttgatatcaa taaattcttc aaacaaagta catactcctt
     7681 tttatctatt atttagactt attccgacag ctacatgaat aacgtacaaa tagattcgtt
     7741 actgattttt cagagatact accatgcgca gtgtgcattg tcaattattc ttgctcttat
     7801 tttccatttg tgttcctgtt atttcctgtt tctcaaatat gttagtaaac aaaaatagat
     7861 ctggcaaagc tacaacttcg ccaggcaata cagcactctc gaatgaaata ttcactcaaa
     7921 aatctatcgt tttattcaat tttagatctg ttgcttttat tctttatttc actcgatgga
     7981 agagagaacg aaaatagctt gctcctacaa tcaaaatgag taacacaccc atccatgtaa
     8041 ttagcttgtt caaggttcta ctaccagttt ctggtaaatc gctttttcta ttatctgttt
     8101 ctttcgcttt ttcttgatga tcaattccac gagaaacaaa atcaggctgt tttagcacac
     8161 tttttaggtt gctccctgca tttctctttt gttccttagt tggtatgata ggtaccaaat
     8221 caagttcatt atttactttt cctgtgctgc tttcgctgct cgtgcttcct gtgctactct
     8281 cactactcat gcttcctgtg ctactttcac tactcgtact ctccgtactg ctttcgctgc
     8341 tcgtgcttcc tgtgctactt tcactactcg tactctccgt actgctttcg ctgctcgtgt
     8401 tttctgtgct actttcactg ctcgtactct ctgtactgct ttcaccgctc gtgttttctg
     8461 tactgctttc actacttgtg ctatccgtac tgcttggcgg tccaacaact gtccaactgc
     8521 ttggtttcaa attctccgtg atcgtcatct gtccatcata aaaaacaatc aaatcaactt
     8581 tatctttact cttgatctca tcaatcgata gttctgcaga aatcattatt gtatactcaa
     8641 taccagcggt ccattgtacg tcttttaaat aaacttcccc aataactttt tctgaacttc
     8701 cattaccatc ccaaccatat gttagaacag catgtacatc actagcattc acgcttttgt
     8761 ttggtttgac tggaatagta atgacttgtt gtgtcatgtc ataaacaatc cgatcagttt
     8821 tcacagtaaa tgaaagattc tcatttgccc gttcatttgc agaagtactg attccaatca
     8881 ttgctgcaat tatactcagc ccaagaataa caaataatac ggtcgctgtt ttcttcattt
     8941 ctatcactct ccatataatt accttctatt tgataaagga attatataaa atgaaaatga
     9001 tgagaacacg acagtcagtt gtgaaaatct aacaaaagat gtcgtgtatt cgcttgaatt
     9061 tgtttacttt ttcgtttaat caatgacaga cttaaaaaag caagaccgat atcagccttg
     9121 ctttcttcat cttttattga taaacagcag tgtatttctt catccgcttt gttttatatg
     9181 catagagtaa caaaccaaca atcaagacta acaagaataa tgtagttgat atagatgctt
     9241 cagccccaaa gtttccccca gtaattattg tattttgcga ttggatcaga gtggagaaaa
     9301 aaccttgttc ttgcgttccg ctgactggta tacccaaaat agctccttgt gtccgattcc
     9361 aagtaatatg agcaccaata gccgtaaata aacttcctgt actgtagata agtgcgctga
     9421 aaaagcaacc tgctaaaaag atattgaaaa ctgccagaaa tgtcatgtta ggattcaatc
     9481 cgtgcaaaag agcaaataat gcagcagatc caatcattgc tattaacgga ccaaatctaa
     9541 gcatctgatg ctgaaaaaag ccacggaata aaagctcttc tgatgccccc actgcaataa
     9601 ataaaataaa actcgttatc agcggcacaa tgtttgcgtt tcctgaatct aaccgatacg
     9661 cccccgtcaa atataatgga ctagtaataa gcagaaacac tccaattcca acaacgagac
     9721 ctcccgctaa atacccccag tcttttcctc gaacagaaac accataatct tctattcttt
     9781 ttccttcgac cttactagca agaaataaag ctagtaaaaa aacagctaaa tgtccgaccg
     9841 taccggcaat tgctccaatc cattcactat ttccaataaa catatccgca agtgcattat
     9901 tgacaagaat tcctaaaaca aaaccaacta agactattaa aacagatcca atcaaccgaa
     9961 taatcctgat catctttcat ccctccttta tattaagttt aacaaccctt ttctttcttg
    10021 tctcgtgaaa actatgtaaa tgttctccat catagagaga atagattttt acgaactaag
    10081 aatatctaca gcaaccttac gaaaatgaaa gactgtctcc ctaattcttc tgttagactg
    10141 tttttaataa ggaaaggatg ggaaattttt tgaaaaaaca actactaatt atgtgccttt
    10201 cagcatgtgc aattttacta ttctcaggtt gtagcaagtc caatgaaacg attgcaacgt
    10261 ttaaaggtgg aacacttaca gttcaggatt tttatgataa cccaaaagtt aaatcaaata
    10321 atcaaacact attaaaaaaa atgattgtct tcaaagcatt tgatgatgta tacggaaaag
    10381 agatttcttc aaaggaagta acaaaagaat ataatgaaca aatcaaaaaa ctcggtccaa
    10441 actacaagga acaattaaaa gcagttggcc aaacagaaga gacatataag aaactcttta
    10501 aacagatgct ggctttccaa tacggtttga aagccaatgt aaaattaaca gataaagatc
    10561 tagacactgc ttggaaagaa ttctatccag aagttagtac acagatcatt ctattttcga
    10621 ctaaagaagg agcagataaa gcaaaaaaag aagcaaatga aggagaaaat ttctccaagc
    10681 ttgttcaagc ttatggaaaa aataaaacat taaaggaaac tgatggaaaa atgaattttg
    10741 attcaaccaa tccagagatt cctactgaag taaaaaaagc agcatttaaa ctaaaaaacg
    10801 gggaagtttc tgatatcatt cctgtaacag atccaacgac ttatcagcaa agctattatc
    10861 tagtaaaaat ggtaaaaaaa caggataaag gttctaataa agacaaatac aaatctgagc
    10921 tagaaaaaat tgctaccgaa gcaaaaactc ttgatacgga attcatggat aaaacaatcc
    10981 gtaaagtgat gaaaaaagac aatgtaacta ttaaagatcc ttatgtaaaa aatatattca
    11041 aataaacaaa tttgacccaa ttggggatgt ggcaaaattt gagaagtgat ttttggaaat
    11101 tccttcttat ttttgaggca aaccactttt gccacaactt catcggggcc tttttttatt
    11161 tgtaccaaaa caaatcactg gatttgttac aatcatgagg atataattta aaaaagtttt
    11221 ttatttctat cacatgaatc aatgcgccat taagtagatt ctttcatgct tccctatttt
    11281 ttcacttgcc gtatcgcttc aaaaacaact atcataaact caaactcgac tgaaaagtta
    11341 ttttaaaaaa gactaaaaat atcccttctg gacttgttct tcttattctg ttatactaga
    11401 gaaacacgaa agatattttt atgcgacact tttcatttgt ttttatgaaa tttattcaaa
    11461 actggcgtaa cttcagagtt acaaggatgt attagaggtt agggaataca tcgtttaaat
    11521 gagaaataag taatagttag taatagtcac ccaaacttta aattaatgaa tattgaatcg
    11581 tcagatagcc tcctcgagtt ttcgagaagg ctatcgttta ttttatggag aaattgattc
    11641 agaagtaatc agcgaccttt tttagttcac caatcgtttc tgcttggaaa gtttgcaccc
    11701 aatcttcttg gaagcccctc actgctttat caatcgaggg catctctaat acttgatcta
    11761 gtagctcttt acctaacgtt atcgcatgag ctccagatcg aatggctgta gtgacttgtt
    11821 cacaattttt gaaactagct gctaatattt ttgttggtct ttgcgtacgt tcgatctcag
    11881 tccgcaacga atcaatcact ttcatagcat caatgccttg attttgcatc ctgttataat
    11941 aaggtgcaat ataatctgct ccattattaa tagctaaata cccttgtaat tctgtgtaaa
    12001 tagctgtacc ggtaatcaaa taaccttctt ttttcaactt tttgatcgct tgaaggcctg
    12061 cttgattcac cggcaccttg ataaacgtat cttttcccag ttcttctatg ataactcgag
    12121 catcctctat catttcttct tccgttcttc ctattgtctg aatatggata ggaatctgtc
    12181 cgattatttt tttgatttct ttcatatgcg agaagaagtt tgacgctcct tcttttttca
    12241 gaattgtagg atttgttgtt acgcctgtta aattcaaaaa ctgttgacaa gaataaatac
    12301 tttttgtatt tgctgtatca agcatgattt ccatatagct ttcccccatt ctgatcctct
    12361 tgatcgattt taatgattgt cccctttctt aaatgcggca tttccttatc ttctacatac
    12421 attgttcctg gaagttccgg ctcatgagat ccatcaaatt ttatcgtgat atgtcctaat
    12481 tcattcaaat tagtttggac aacatctcct actgctgtga ttacatacat ttgtttatca
    12541 aaacagagct tcatatcaca agttatttct tttcttacct gattcaattc aatcttataa
    12601 caataatctg ttaatgtttc cggtgcttca tctccaaata aaatcattat tttttctttc
    12661 ctgaatactt ctgcttctat accaacatct gttacaaccg tttcaaagat cgtcatc
//
LOCUS       DAHHZJ010000076        12683 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.077, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000076 DAHHZJ010000000
VERSION     DAHHZJ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.077"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            24..911
                     /locus_tag="NBL02_001664"
     CDS             24..911
                     /locus_tag="NBL02_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HCD2572348.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTRAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            908..2077
                     /locus_tag="NBL02_001665"
     CDS             908..2077
                     /locus_tag="NBL02_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572349.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            2101..3456
                     /locus_tag="NBL02_001666"
     CDS             2101..3456
                     /locus_tag="NBL02_001666"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCD2572350.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(3528..5333)
                     /gene="glmS"
                     /locus_tag="NBL02_001667"
     CDS             complement(3528..5333)
                     /gene="glmS"
                     /locus_tag="NBL02_001667"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCD2572351.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            5743..6033
                     /locus_tag="NBL02_001668"
     CDS             5743..6033
                     /locus_tag="NBL02_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572352.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            6046..7071
                     /gene="sppA"
                     /locus_tag="NBL02_001669"
     CDS             6046..7071
                     /gene="sppA"
                     /locus_tag="NBL02_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCD2572353.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            7074..7733
                     /locus_tag="NBL02_001670"
     CDS             7074..7733
                     /locus_tag="NBL02_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HCD2572354.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            7890..8822
                     /locus_tag="NBL02_001671"
     CDS             7890..8822
                     /locus_tag="NBL02_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572355.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            8976..10265
                     /locus_tag="NBL02_001672"
     CDS             8976..10265
                     /locus_tag="NBL02_001672"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCD2572356.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            10512..11762
                     /gene="clpX"
                     /locus_tag="NBL02_001673"
     CDS             10512..11762
                     /gene="clpX"
                     /locus_tag="NBL02_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HCD2572357.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            11845..12432
                     /locus_tag="NBL02_001674"
     CDS             11845..12432
                     /locus_tag="NBL02_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCD2572358.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(12506..12661)
                     /locus_tag="NBL02_001675"
     CDS             complement(12506..12661)
                     /locus_tag="NBL02_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572359.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
BASE COUNT         4152 a         2243 c         2772 g         3516 t
ORIGIN      
        1 tcaaaagaga agagaggctt tttatgtcat ttcaaatcgg ggaacttttt aatttggact
       61 attggcagca aatgttttcc ggtgacttgt tctctctgaa ttttttggtg aacatacttg
      121 atattctggt tgtctggtat cttgtataca aactgatcca actgctgcga ggcacaagag
      181 cgattcagct tctgaaaggt gtagctattt ttattgttat ccgaatcgta gcggaaatca
      241 ttggtctgca caccttgtct tggctgatga accaagtcat tacgtacggg gtgatagctg
      301 ctatcgtgat ttttcagccg gaaatccgga gaggactgga acatcttgga cgcagttcgc
      361 ttttcaaaca atcaaaaagc gaacagcagg aagatgagaa gatgatcttg tcttttgaca
      421 aagccatcca atatatgtcc aagcgaaaaa tcggtgcgtt gatcactatc gaacgtcata
      481 ctggattaga tgagtatatt gagacaggga ttgctttaga tgcagatatc actggggagc
      541 tgctgatcaa tatctttatt cctaatacac cgctgcatga cggtgcagtt attgtaaaag
      601 aaggaaagat cgcagtagca agcgcctatc ttcctctatc agaaagtatg ttgatcccta
      661 aagaattcgg gactcgacac cgggcagctg tcggtataag cgaagtcagt gacgcgatta
      721 ccatcgttgt ctctgaagaa accggggacg tcagcatcac gttagataat gaactgatgg
      781 ctggcttatc ccagcaagaa tatttggcaa tcttgcgtag agaactaatt gctgaagaga
      841 aaacaaaaaa caagaaaaac gtgattcaat ctttcctaga cggtatgact aagggagggg
      901 atcgcaaatg atctcaaaag agaaacggac aagcattctt tacagtttta ttgcattgct
      961 gttcagtatt gcgctgtact tcaatgcaaa tggccagtcc gttccaaata cgctttctgg
     1021 aaatgaagcg tataatcaaa cagttacggg ggtgccgatc aagatttctt atgattcaaa
     1081 gcgctactat atccatggat atgaaaatgc agtgactgtc aaactttcca gtgcaaaccg
     1141 agttcagttg aatgcagagg caaacgaaga tacacggatg ttccgcgtca cagcagatat
     1201 cactaagctg ggcgaaggaa cacatgaagt tccgctaaaa attcaaaatt tgagcagtgc
     1261 tgtcacagca aagatcgaac catcgactat tacagtaacg gttgagaaaa aggtaataaa
     1321 agatttcgat gtggaaacgt tgattccttc aacgttcaca ccatctggat atgaacttga
     1381 tgatacttcc gttagtccta aaaaagtctc tatcactaca ggtgacaaaa cattggcaga
     1441 aatcaaacga gtagtagctg atgtggacgc ttcgatggtc acagatgatg ggattaatag
     1501 tgaggtccca atacaggcgc tgaatgcagc aggggaagta ttaagtatcg tctctgatcc
     1561 agtgcaagta acagtgagag ctgattcgat caagccaagc aagtcggtac gactttatgg
     1621 gatacagcaa ggaacaccag ctgccggggt agaaagctat gatttcagtt tttcaaaact
     1681 ggaagcagaa gtaagcggct ctagcgattt gcttgcatcg atcggtgaca gtattgctgt
     1741 accgatcaat gtctcaggta tcaatcatcg aacaactcgt aaaattgaaa taccagtaga
     1801 agaggggtta agtatcaagc ccgaatcagt tagtgtggag attacacccg tgttgcaaac
     1861 gtcaagcagt agctctgctt cgacgagcag tcggacacaa gaaaatacaa gtacgagtag
     1921 aagtatttca tctgctgatt caggaacgga accatcgaca acaaccattt cttccagttc
     1981 ggaatctggg acaagcagct cgaccacaag tacagaagaa acatcagaaa caagcgaatc
     2041 aacacaaatg agcagaacaa attcctcaga aaattaagtt aaaggagtaa ttttacaact
     2101 atgggtaaat attttggaac agacggcgta cgcggtgaag ccaacaagga acttacgcca
     2161 gaacttgcat ttaagctggg acggtgcgga ggttatgtcc taagccagca tgaagaagaa
     2221 ggaaaacgcc cacgcgtgtt agtagggcgg gatacaagaa tctcaggtca aatgttagaa
     2281 gaagcgctga tttcaggact tttatcggtg ggtatcgaag tgttccagct aggtgtgatc
     2341 tctacaccag gtgttgctta tctgacacgt ttgcaaaaag caagcgcagg ggtcatgatt
     2401 tctgcttctc ataatccagc acaagataat ggaatcaaat ttttcggtgc agatggtttt
     2461 aaactagtag atgaccaaga gctagaaatt gaagcattat tagatgctga agtggatgaa
     2521 ttgcctcgtc catcagctga aggattggga actgtagaag aatttccaga aggcttattg
     2581 aaatattctc aattccttgt acaaacgatc aacggtgacc tttccggttt gaccatttgt
     2641 gtcgatgcag caaacggagc aacttcaaca agtgttaatc gtttgtttgc cgatcttgaa
     2701 acagattttt atacaatggg cacaaatcca gacgggttaa atatcaatga cggcgtaggc
     2761 tctacccatc cagaacgttt agctgagatg gtcgttgaaa aaggagcaga tgctggacta
     2821 gctttcgatg gcgatggcga tcgtattatt gcggtggatg aacttggaaa cattgtcgac
     2881 ggtgataaaa tcatgtttat ctgtgctaaa tacttggcag aaaaaaatcg attgaaaaaa
     2941 gatacgatcg ttacgactgt catgagtaac ttaggattcc acaaagcggt ggaaggaatc
     3001 ggtttgaaag atgtcgttac acaagttggt gatcgttacg ttgtagaaga aatgcgcaaa
     3061 aacgattata actttggcgg cgagcagtct gggcacatga ttttcttaga ttttaacaca
     3121 actggagatg ggatgctttc aggaattcag ttactgaata tcatgaaaca gacaggtaag
     3181 aaattatcag agcttgcagc agaggtaaca atctatccgc aaaaattagt gaatatccga
     3241 gtaagtgata aaaacggtgc gatggatgtt ccggcaatca aaaaagtgat tgaagaggca
     3301 gaagctgaaa tgggatcaga aggaagaatc ctagttcgtc cttccggtac agaaccatta
     3361 cttcgagtga tggcagaagc gcctactgat gagaaagtta attactatgt cgataaaatc
     3421 gcggcagtag tcaaagaaga aatcggactg gattaagaga tattttcata aaaaataaaa
     3481 gaacaaggac cagtgtcaga tgtctgacac tggtccttgt tcttttatta ttctactgtc
     3541 actgatttcg ctaaattacg tggtttgtca acgtcgaatc cccgtaatag cgttgcgtaa
     3601 tatgcaagta attgagtcgg tacgactgtg gttagagtag ttagataagg gtggacagtt
     3661 ggtagcacca gctggtcact ttccttttct aatccctctg agacaataac tagtgtttta
     3721 gcgccacggc tttcgacttc tttcaggttt cctcgagtat gcgcaccagt cacttcttca
     3781 ctgatgattc caatcactgg tgttccttct tcgatcaaag caatcgttcc atgtttcaat
     3841 tcgccagcgg caaaaccttc tgcctggata taactgattt ctttcaattt caaagcagct
     3901 tcaagagaaa cgttgtagtc agcgccacgt ccaatgtaaa aggcatttct tgtatcgctg
     3961 agatatactt ctgccaactc ttctaaataa tctttttggg cgatgaccga ttccatcgca
     4021 ttagcaacta aactcaattc atgagtgata tcgaatacaa gagaagtttc aaattctttt
     4081 ttatcaccga tagctttggc taataaagtt agtacagcta tttgtgcagt ataagctttc
     4141 gttgatgcta ctgcgatttc gggtccagca tgcaatagca gtgtaaagga tgcttcccgc
     4201 gaaagagtag aaccagcaac atttgtaatc gtaagtgaag ggtagccgag acggttgatt
     4261 tttaccagaa cttggcggct atctgctgtt tcgccacttt gactcaagaa aataaagaat
     4321 ggtttttttg aaagaagagg tgtgttatag ccaaattcac tggctaaatg aacctcaaca
     4381 gggatctgtg tcaatttttc cagagtctgt ttacctgcta accccgcatg gtaactagtt
     4441 ccgcaagcaa cgatatagat tcgatcgcta tcagacaatg tatcgaccag cttttgatca
     4501 agagagacac gattgtcttc tgttacgtat ttttgagcga tacgacgcat gataataggc
     4561 tgttcgtcga tttctttcag catataaaaa ggataggtac ctttctcgat atctgaagaa
     4621 tctacttgtg cttcgaatga agaacgttct acgatgtcac ctgcaaaatt ttgaataata
     4681 actttttctg atgtgacggt aaccatctct tcatcttcaa tctcaacgaa ttccttcgta
     4741 tgagcaagca tagccattgc atcgctggca atcacattga atccatcgcc gagtccgatt
     4801 aatagtggac ttttattttt agcgacgtaa ataacatcag gtgctgtccg atcaatcaaa
     4861 gcgaaagcgt aagaaccttt gatgacccgc aaagctttta aaaacgcctc ttgcgttgta
     4921 gtatccttcg cgaaagtttc aattagatga gcgataatct cagtatcggt ctcaccaata
     4981 aagtggtcat ttgctaaata agcaatttta agttcttcaa agttttcgat tacgccattg
     5041 tgaacgagca cgaagcgtcc actttgggaa gtatgtggat gggcgttttc ttcagaaggt
     5101 tgcccgtgag ttgcccagcg tgtatgacca atacctatat ttcctgtagt atcaatattt
     5161 attttgtttt tcaaattttg aattctgcct tgtgctttga ccagataatc agttgtgcca
     5221 tctgagacaa agataccagc tgaatcataa ccacggtatt ctaatttttc tagaccgtca
     5281 atcaatcctg gtgttacatt ttttaatcca atcattccaa caattccgca cataaaaaac
     5341 atcctacttt ctataatgaa ttcgtatgaa tgcttccagc tgcaggaaaa tccttttgtt
     5401 ttatctttgc agatattttt tgtggatttt ctgtagagtg cagtccctag ggccatccgc
     5461 cgaatctttc gataaaccct ctcctcgtca tctgtttaca cagacctggc gctatcctaa
     5521 aaggtataac tatcctcctc ttttgttttt tatctggaaa catttaaaat cataaccttt
     5581 tgcgaatgtt cttgtcaaca aaaaaagaat aaaaaataaa ttggtatata tcaaatgctt
     5641 tgtcaaatgc tctttttatg tcaaaaaata atgggttttt atctatcatc tttttcagga
     5701 gttcggtaca atagaaagta atcggataga ggaggagtaa aaatggattc aaaaattgag
     5761 atcatgacac tgggaatgtt aaaaaaacaa ttgtccaaat tcgaagcatc agctggtgta
     5821 tcagatgata cgaaaatatt tttagatact ggctgggata gcatccaaga aatcgcacct
     5881 gatgccttgg aagttgctga agctcgtgag tttacggtag aagatgaatt gacaaaagag
     5941 tcgttttccg ggtatgcgcg ggaagaaaaa gcagagcggt tcgacacttc tgagcagtca
     6001 gaaactgtga tcgtcataaa aaacttgtat tagaaaggag aaagaatgaa taagagacgt
     6061 tgggcagcag ttctgattgc tgcaggatta tttgtggttt cgttagtttc agctagtttg
     6121 actaagcctc aggaaaacga tcaaattgag ggattgaaca gttggttata tggggatgaa
     6181 gaactagctt caaatgtgtt ggaagaggga gacagtacgc aaaaaatcgt aaaattatca
     6241 gtagatggaa cgattgctgc gggagggtct tcaggtttat ttgcttctga aggttacaat
     6301 cataaaaatt ttatggaaca actgaaggcg attgaagaag atccaacagt caaaggtatc
     6361 tttttagaag taaattctcc tggtggcggt gtctacgaaa gtgcggaaat cgctaagaaa
     6421 ttggatacga ttcgtaaaga acatgatatc ccaatgtatg tcagcatgaa aaacatggca
     6481 gctagcggtg gctactatat ttctgctcaa gcagataaaa tctttgcaac agaagaaaca
     6541 gtgaccggct caattggtgt gatcatgtca ggattgaatt attcaggact tctagaaaaa
     6601 ctgggtgtgg aagatacaac agttaaaagc ggagcgttga aagatatggg ttcaggaacg
     6661 cgaccagaga caaaagaaga agaagcggtg cttcaagcat atatcgacag cgcatatagt
     6721 cgttttgtag caatcgtcag cgagggacgg aataaatctg aagaagaagt caaaaaaatt
     6781 gctgatggaa ggatttatga tggtgtccaa gcaaaagaag taggtttagt cgatgaattg
     6841 ggatttcctg aagatgcttt aaaagctatg cgaaaggaac aacagcttga agatgcggaa
     6901 ctagtagaat attcttccac gccatctggg tttgctagta catggttagg aagtaaacta
     6961 gcagaattcc agggattgaa gccttctgag actagtcaga tcttatccgt tcttgagaat
     7021 ctagggacag cggaatcacc aaaagcaatg tattattacg gaggtgagta agcatgtcag
     7081 agctttcaaa tgaaaataag gaacaagaaa aagataagca acagcagcct gctgtaggac
     7141 ctgactccaa cttttcccaa aaggtactga gctcttttga aagagagcct ttgacgcccg
     7201 aagaaatcaa aaagaaacaa cgtaaatgga aaaagtatac ggacacaaga aatgaaccgc
     7261 caaaagcaaa tgattttcct aagtatttct atgcaggttt ttttatccga atgtttgctt
     7321 tcttggtcga tcttttgtgt atcggagcta ttacgcgttt aacacttggt attgccaaaa
     7381 atctaggttg gcttaatttt tcagattctt atctgagtat ctacggattc acagccttac
     7441 ttatttatct agcgtatttt attttattga cgaaattcaa tcatggtcaa acgattggga
     7501 aaatgatctt tggtattcga gtcatcagct ttgatgaaga agaacttagc tggtcaaccg
     7561 ttttagttag agaaggtgct tgccggttca ttctaaaatt tcctttgctg atgttagggt
     7621 atcttccagc aatctttaat cagaaaaagc aacatctagg cgattttttc actaatacaa
     7681 gtgttgttac gatcaatctg atcaaagcat tcaaccagga agtcaacagc tgatgagcaa
     7741 taaaaagcag taacagttga aagcaccttc taaaagtcag atctaaaatc tagcttttag
     7801 agggtgcttc agttattttt aattgtcatg ctttttttgt tttgctacta ttgaacatga
     7861 gctgaacatg agcaagagga ggacactgcg tgaagccgat tgattttcca gaaaaatatg
     7921 agaatttaat gcgagtagca caacaagcac tagcaaacca gcaatatcag caggcaaaag
     7981 aattatttca acgagcttac gagttgaaag aaagctttga ggctaatagc ctgcttgttt
     8041 tttgtcttta cgagttggac gagaaaaaag aagcattgaa gcaagcactc ttgcatgaaa
     8101 aacagtatct tcaaaatgag gagtttgctg aattttattt tgacttgtta atcagtacac
     8161 aagatttttt atatgctaga aaattgatag caagtacaga tttttatgaa gctttcgagc
     8221 agcgggtcat tgaaaaaatc caatttgcag aagaattatc cggacaaatg gagaggcaga
     8281 agatcaaggc actccacaat aaatcggagg aacttccttc gttggaacct gcacgtcagc
     8341 ttgcgtctat tgatgagatc gaacagcttc cttatcatga gtttatccaa acagcgagta
     8401 aacttattgt atcgccagag gtccatattt tagcaagggc aaaattactg gagacactta
     8461 ggcaattgaa cgaatgccat cctgttttct atttgacgat tgaagaaaag ttagtcaaag
     8521 tcataccaaa agatctgcca aagccgcagc agcaggcaag ttaccagcaa ttatgtgtat
     8581 tttcagatcg ttacggtaat gaagatgcgc tgcttagttc cgtgttaaat gaagaattca
     8641 cgttgcaatc tgcaattgtt tatcctgtct atgatacata catcgaagac ccgaagcgct
     8701 ggtttcaact gactgtagag gcgtgtacag gaaaaacgat gtatgaaggt acagaagatg
     8761 aaaaacaaga ttttttaaaa aaaagagaaa aaattttgca gcaaatgatc ttcttccatt
     8821 agattttatc agtgaaaaag ataaaaatgt tatgaaaaac aaggattcta tccattcaat
     8881 atgtttacaa tttgaaaggg ttagtgtact atttaaaggt atgcatgaaa cgtttttgat
     8941 gtataaaatg aatttctaaa ctcggaggga attaaatgac tgcaacattt gaaaaaaaag
     9001 gcacgaatga tggtgtttta aaatttacga ttgcgcaaga cgaaatccaa aaaggattaa
     9061 caactgcgtt taataaagtg aaaaaaagct tgaacgtacc tggattccgt aaagggaaag
     9121 tttctcgtca agtattcaac cgtatgtacg gtgaagaagc tttatatgaa gatgcattga
     9181 atgctgtatt accagaagct tatgaagctg ctgtcaaaga agctggcatc gatcctgttt
     9241 ctcaaccaaa aatcgatgtt gaaagcatgg aaaaaggtca agactgggta atcactgctg
     9301 aagtaacagt gaaaccagaa gtaaaattag gacaatataa agatctagtt gttgaaaaac
     9361 aagaccgtga agtaacagat gctgacgtgg atgcacgtat caaacgtgaa caagaagcac
     9421 aagctgaatt agttatcaaa gaagatgctg cagctgaaaa tggcgataca gtagtaatcg
     9481 acttcgaagg ctttgtagat ggcgaagcgt ttgatggcgg aaaaggcgaa aattactcac
     9541 ttgaattagg ttcaggctca ttcattccag gttttgaaga tcaattagtt ggtcataaat
     9601 caggtgaaag tctagatgta aatgtaactt tcccagaaga ttatcaagca gaagatcttg
     9661 ctggaaaaga agcagtcttc aaagtgacaa tccatgaagt aaaagcaaaa gaagtgcctg
     9721 aattagatga cgaattcgca aaagatgttg atgacgaagt atcaacatta gacgaattga
     9781 aagaaaaata tcgcaaagaa ttagtagaaa caaaagaaaa agcagcggac gatgcaaaag
     9841 atgaagcagc gatccgtatg gctgttgaaa acgcagaaat cgttgaattg ccacacgtga
     9901 tggttcatga tgaagtacat cgttcaatgg acgaattctt gaacaacatg caacgtcaag
     9961 gtatctctcc tgaaatgtac tatcaattaa ctggttctac agaagaagac ttgcataaac
    10021 aattcgaagg tgaagcagaa atgcgcacaa gaacaaatct tgtgatcgaa gcaattgcag
    10081 ctgctgaaaa tcttcaagca acagaagaag aaatcaatgc agaaatccaa gaattatcag
    10141 aaacatacaa catgccaatc gaacaaatca aacgcgtgtt gacagaagat atgctggaac
    10201 atgatgtgac aatgaaaaaa gcaatcgaat tgatcacagg tacagcgaca gaagcgaaag
    10261 aatagaaatc aagtaataaa tcatttctga agaagctact tctagaaaat tgtttattcg
    10321 aaataaggga ctgtgacacg attttgtcac agttccttat tttttgttaa gagtaaaaaa
    10381 tgcaaaggta taggtcagtt gatcctttgt taaacacttt ttctttttac taaactatgt
    10441 tatgatagta aaagactgta aagtctttga tagtttcttg catacttttt gcaagttaag
    10501 gggtgaagac catgtatgac aatccaagta gcaatgagac cgtacgctgt tctttttgcg
    10561 ggaaaaccca agaagaagtg aaaaagatcg tagctggtcc aggggtctac atctgtaatg
    10621 aatgtatcga cctatgtaaa gaaatcattg atgaagaatt ttatgaagaa gctgtacgcg
    10681 aattcactga agtccctaaa ccactagaaa ttcttgaagt attgaataac tacgtgattg
    10741 gacaagatcg cgcaaaacgc tcgttagctg ttgccgtata caatcactat aaacgtgtca
    10801 atcagcaagc acaagaaaac acagatgatg ttgaactgca aaaaagcaat atctgcttga
    10861 tcggacctac agggtctggt aaaacatttt tagcacaaac attagcaaaa acattgaatg
    10921 taccttttgc aatcgcagat gcgacaagtt tgacagaagc tggttatgtg ggggaagatg
    10981 tagaaaacat tttgcttaaa ttgcttcaat ctgctgatta caatgtggaa cgtgcagaaa
    11041 aagggatcat ctatattgat gagatcgata aaattgcgcg taaaagcgaa aatgtatcta
    11101 ttactcgtga cgtttccggc gaaggtgtcc aacaagcctt gctgaaaatt ctagaaggaa
    11161 ctgttgcaag cgtgccacct cagggaggac gtaaacatcc acatcaagaa tttattcaga
    11221 ttgatacgac gaacatattg ttcatcgtag gtggtgcatt tgatggcatc gaaacgattg
    11281 tgaagaatcg tatgggcgaa aagacgattg gatttggtac acaaaaccaa aaactttctg
    11341 aagacgaaag tgtcatgcag catatcattc ctgaagattt actaaaattc ggattgatcc
    11401 ctgagtttat cggacgatta cctgttatgg cagctttaga gaaattgaca acagatgatc
    11461 tggtacggat cttgactgag ccgaaaaatg cgctagtcaa acaatatcaa aaacttcttt
    11521 ctctagataa taccgaactt gaattcgaac ctgaagcgtt gagagccatt gcgaaaaaag
    11581 caatcgaacg caataccggt gctcgaggac ttcgttccat cattgaagaa atcatgatgg
    11641 atgtgatgtt tgatatccct tcaaatgaaa acatcgaaaa agtgattatc acaaaagaag
    11701 cagctgaatt gtctggtgag ccaacagtaa tctataaaga ttcagagaaa aaagcgggtt
    11761 gatgaaaaga ggctgccgta aaagtcattt tggcttttgc gacagccgct tgctttattc
    11821 atcaccttgt taaggagaga gaccatgaaa gtccatcaag ctgaaattat cataagtgca
    11881 gtggcaccaa aacaatatcc agatacacaa ttgccagaaa tagctctggc aggacgatca
    11941 aatgtaggga aatcttcttt tatcaacaca ttgattgatc gaaagaattt agcccgtact
    12001 tccggtaagc caggaaagac ccagacattg aatttttatt tgatcgagaa cgcattacat
    12061 tttgtcgatg ttccgggata tgggtatgca aaagtatcca aaactgagcg agcaaaatgg
    12121 ggaaagatga tcgagacata tttaacgact agagaccagc tacgtgcggt cgtttcttta
    12181 gtagatttgc gtcataagcc ttctcaagaa gatatccaga tgtatcaatt tttgaagtat
    12241 tatgatatcc ctgtaattgt tgttgccact aaggcagata agatccctcg tggtaaatgg
    12301 aacaagcatg agagcgaaat caaaaaagcg ttggattttg atcgcaatga cacgtttatt
    12361 ctcttttctt ccgttacaaa acagggaaaa gaagaagcgt gggcagcaat cgaacaagca
    12421 attgaatcat aaaaagaata aaaaagactt accagtcaaa ctctttcata aaagtttggc
    12481 tggtaagtct ttttttgagc ttaacttact tttgtttctt gtcttttttc ttttcatttt
    12541 gtggctgtgg ttttagaaat cgatcgatct cttcttttgg tgcaactgtt ttttccggat
    12601 cgacagcaaa tgattcgaaa agtttatcta aattatctgt acgttcaaat ttcaacccca
    12661 ttgtaaacac ctctttgttt ctt
//
LOCUS       DAHHZJ010000077        12595 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.078, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000077 DAHHZJ010000000
VERSION     DAHHZJ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.078"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            52..904
                     /locus_tag="NBL02_001676"
                     /pseudo
     CDS             52..904
                     /locus_tag="NBL02_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            1001..2887
                     /locus_tag="NBL02_001677"
     CDS             1001..2887
                     /locus_tag="NBL02_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCD2572360.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFETPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(2928..3830)
                     /locus_tag="NBL02_001678"
     CDS             complement(2928..3830)
                     /locus_tag="NBL02_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCD2572361.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            4060..5463
                     /locus_tag="NBL02_001679"
     CDS             4060..5463
                     /locus_tag="NBL02_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCD2572362.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            5460..6896
                     /locus_tag="NBL02_001680"
     CDS             5460..6896
                     /locus_tag="NBL02_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCD2572363.1"
                     /translation="MMGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFP
                     VITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGD
                     EKEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENL
                     CRVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASA
                     IATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPA
                     YALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFAS
                     VKNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDY
                     RIDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGN
                     GTLARSKKKSFNWYKKVIDSNGENLSND"
     gene            7041..7967
                     /gene="trxB"
                     /locus_tag="NBL02_001681"
     CDS             7041..7967
                     /gene="trxB"
                     /locus_tag="NBL02_001681"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCD2572364.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            8181..9908
                     /locus_tag="NBL02_001682"
     CDS             8181..9908
                     /locus_tag="NBL02_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HCD2572365.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIQKMTEE"
     gene            9924..10274
                     /locus_tag="NBL02_001683"
     CDS             9924..10274
                     /locus_tag="NBL02_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCD2572366.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(10369..11229)
                     /locus_tag="NBL02_001684"
     CDS             complement(10369..11229)
                     /locus_tag="NBL02_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HCD2572367.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            11321..11749
                     /locus_tag="NBL02_001685"
     CDS             11321..11749
                     /locus_tag="NBL02_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCD2572368.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            11911..12087
                     /locus_tag="NBL02_001686"
     CDS             11911..12087
                     /locus_tag="NBL02_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCD2572369.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            12114..12560
                     /locus_tag="NBL02_001687"
     CDS             12114..12560
                     /locus_tag="NBL02_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HCD2572370.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         4119 a         2171 c         2775 g         3530 t
ORIGIN      
        1 ttttcttttt gttttggata cagccctata tatcatggaa aggagcaaaa catgctgatt
       61 gagaaagtac tgaataacaa cgtcgtaata accagggatg aaagaaatca ggaaatgatc
      121 gtgatgggaa aaggtttggc attcaaaaag aaagtgggtg acccgatttt tgactcatgc
      181 atcgacaaaa aatttcagtt gacaaatgac gggctttcac aacagttcca agaattggca
      241 caagcgattc ctcttgagta cttagaaatt tcttgtgaaa tcattgaata tacaaaagag
      301 gctctacaag tccagttgaa tgattctgtg tatattgcat tgacggatca tttgtatacg
      361 gctatcgaac gtcacaaaat gggaatattt gtaccaaatg tattgctgta tgatatcaaa
      421 caactatttc ctaaagagta tgccatcggg aaaaaaaaca gtgaagaaaa tctcggataa
      481 ataccacatc aagttatcag aagatgaagc cggatttatc gcattgcatt tagtcaatgc
      541 acaaggcatt caaagccaac aagacaatac ctacctgttc actataacaa tacaggatat
      601 tctaaatatc gtccgctatt actataagat tacatttgat gaagattcag tccatttttt
      661 tcgattcgtc acacacttac gtttttttct gacacgcgtc ctagatggag aaaaatcaga
      721 agtgggagaa gtttctgagg aattgtttca ggttattcaa aaaaaatata aggatacgtt
      781 tggttgcgtt gagaaaatag cagacttttt agccgagacg ttcaactacc agatgtccac
      841 ggacgaaaaa atgtatttaa cgatccatac tgcgcgttta gtgcatgaga atcgttgtaa
      901 ataaataaaa aaaggaggat aaattaacaa gaacgtatga aataagggta gtgacattca
      961 gttggctaaa ccttccacta ataaattgga gggaatagaa atgggaaaat accaagaatt
     1021 agcgagaaaa atcgtagaaa atgtcggcgg gaaggaaaat atcaatggtt tgacacattg
     1081 tatcacaagg cttcgtttca aattaaaaaa cgaagaaaaa gcaaatgatg agatcttgaa
     1141 aaatatggac ggtattgtaa cggtaatgcg agcgggaggg cagtatcaag ttgtcatcgg
     1201 gaatcatgtg ccagtcgtgt ttgaagaggt tataaaagca ggaaacctta cttttgatga
     1261 ggctgtttca acagaaaaaa tgagaccgtt tgatatgctt attgatggaa tatcgggctg
     1321 tttccaacca ttccttgcta tcctagctgc tgacgggatg attcgtggac tgacagcatt
     1381 ccttgtcttc ttaggcgcgt ttgaccgcgg ttctggcaca tttgttatgt ttgataatat
     1441 cggtgattct gtattccaat ttatgcctgt tattatcggt ttaacagctg cccgtaaatt
     1501 taaagtaaat gaattcgtag ggatgttgct tggcgcagca ttaatgaatc ctagcttgtc
     1561 gctggaagct ttgagtggtg cagcagaagc accactaacg accatatttt ctggcactat
     1621 ttttgaaaca cctatttatc aaacagtatt cggcattccc tggattgcgc gtaactacgc
     1681 ttcttctgtc attcccatta tctttattgt gttgttagct agccaaattc agaaaccaat
     1741 taaaaagtta gtacctgaga tgatcgcgaa tttcttcgtt ccattcttca ccgttcttat
     1801 tacaatgcct ttaggttttt tactcgttgg accagtattc acattcgcaa ctgatatttt
     1861 aatggctgga tttgaaacac ttttagcact ttcgccagtt atttatggag caatcgtagg
     1921 cttcttctgg caaattctgg tgatgtttgg tctccattgg gcaatcgtac caatgggatt
     1981 gatgcaattt tctgtgaatg gttggcaaaa tattatgaca cctgttgcag tagtgtcttt
     2041 tggacaaact gcagcactta ccgcactcta ctttaagctt agaaatccaa aagacaaagc
     2101 aattgcaata ccagccattg tttctggtat tgtggggatt acagagccag ctatttatgg
     2161 gtttacattg ccacgtaaaa aaatatttat ttttacctgt gtcggtggtg cgttaggggg
     2221 cgcgtatagt gggttaatga atttaacttc ttggaatcaa ggtggactag gcatctttac
     2281 aattcccaac tatattagac cagatggtga tttaacagat gtgattaatg tattgattgg
     2341 gatcgccatt gcgatggtgg tatcctttgc gctgactttt ttcttttgga aggaggaagc
     2401 agatgaaaca gatgatagac agaaaagatc aggtaaagaa attgtaaaaa caccgataca
     2461 aggacaaatc gctccactga atgcagcaaa ggatgcagct tttgcacaag gaacactagg
     2521 tcgaggaatc ttgatctacc ctgaaaaagg agaagtacgt gccccttttg acggaacagt
     2581 catgtccctg tttccgacga aacatgcaat cgggatggtt tctgaagcag gtctggaatt
     2641 gctgattcat gtcggattgg atactgttca gttggaaggg aaatattttg aaagcttggt
     2701 gcagcaagga gaaagagtga aacaaggcga tctgttgctt cgcttcgaca aaaagaaaat
     2761 cgaagaggaa ggatacagtt tagaaacacc tgtgattgtc atcaattaca tggattattt
     2821 ggatattttg gaaaatcaag gggaaacggt cgggccagac gatgcactta tcaccgcact
     2881 gacctgaacg gttaggtgaa agaatggact aggcttaact attcttttta gtccacttct
     2941 ttattttcaa aattgaaagg ctctttcatg atggctgagc tgatagggcg tttatcaaat
     3001 ttttgtccta cagaaattag atggactaaa ttctgttgat aattttctgc aaaaacacaa
     3061 ctatataaca cgtctagaag ataacaaatc gatgtattga tcgtaaaatt accaattttc
     3121 gaatacagtc gttcgcgtgt cgtaagtcgc aaagcggctg tactcattga agatagcgtt
     3181 gaatcaccga tactggtcaa agctaaaatt ggcacatgat tttgtttcaa tatttctgcg
     3241 gtacgaagga tcacattgtt ttcacccgta taagaaatca gaatcccaca catatcactt
     3301 gtgcaattgt acgcttcata gaacgattca ccaaaagtgg tgacaatcgt gacatccatt
     3361 ttgatgcgct tcattttcag catgaaatct tgagaaatca aagtattcga gttactggcg
     3421 aaaatcacga ttttttttgc ttgaagcatt agttgtttgg ctttttgcag ttggtcatga
     3481 tgaatgagag aaagtgtatc ttgaatcgtc atctgttcta aagaagccat tttatttgcg
     3541 atcgtcataa taccgtctgt ggtggaaaaa ggaagattcg catctacgtc ttgaaaattc
     3601 ccttcaagat aggctgtttc agctaaaaaa gcattacgaa aactgaccca gccatcaaaa
     3661 cccatttttt ttgctacacg gatgagtgtg gacgggtgga cataacatcg ctcagcaatc
     3721 tcttggatgg tcatttcgcc aagtgctgga gaatcgctta agagaaactg aatcacttca
     3781 ttttctgaag gagaaaagga gatttctttg attttttcac gtaataacat gtagatacct
     3841 tcttcttgtt tttttacgta aattataact tttttgaaaa tgatttgtaa acggatttat
     3901 gttatatagc ttaataaaga cattttttga caagatattt acaaaaatcc agaagaaaac
     3961 actaactttt gtaaatgtta ctggaaagaa acgaatttct attgtttgtt ttgtttacaa
     4021 aaaagataat aaaagtgtaa aaagtaaagg agtgagaaca tggcaaaagg aatcaaaatc
     4081 gctacgattg gtggaggcag cagttacacc cccgagttga tggaaggatt tatcaaaaga
     4141 tatgaggaat taccgatacg agaaatctgg ctcgtagatg tagaagaagg aaaagaaaag
     4201 cttgagatcg tcagtcagat gtctcagcgt atgtgggatg cttctccgta tgaagtcaaa
     4261 atctatgcaa ctctggatcg ggaagaagca ttgaaagatg cggattttgt tacgactcag
     4321 tttcgtgtcg gtcaattaga tgcacgaatc aaagacgagc ggatccctag ttattatggg
     4381 atgattgggc aagaaacaaa tggtgctggc gggatattca aagcttttcg taccgttcca
     4441 gtggttttgg caatcgttga agacatgaag cgtttatgtc caaatgcttg gctgattaat
     4501 ttcgcgaacc caagcgggat ggtgacagaa gcagttatca gatatggcaa atgggataaa
     4561 gtgatcggat tatgcaatgt gccagtaatg gcgatgatga cagaaccaac aatgcttggc
     4621 aagaaaaaag aagagctgat ctacaagttt gccggtctaa atcacttcca ttggcacaaa
     4681 gtagcagata ataaaggaaa tgaccgcacg gcagaattga ttgatcgttt atacgatgaa
     4741 gacaatggat tgccgaaaaa tatctttgac atcccgtttt tcaaagaaca attaaaacaa
     4801 atgaacatga tcccttgtgg ctaccaccgc tattactacc gtgaagaaga aatgctggaa
     4861 catgctttag aagaatatca aacgattggt acaagagcgc agcaagtgaa agaaacagaa
     4921 gcagagctat ttgaattata caaagatcca gaattagacc ataaacctga acaattgcaa
     4981 aaacgaggtg gcgcttatta ttcagacgct gcctgtgaaa cgattgcttc aatctatgga
     5041 aataaagaaa cacagatcgt ggtatctaca aagaatgatg gagctgttcc tgacctgccg
     5101 gcagattgtg tagtagaagt cactgcctat gttggcgcac aaggagcgag aaatgtagcg
     5161 tttggttctc ttcctgctgc tgaaagaggc tggttgcaag taatgaagaa tatggaattg
     5221 ctgacgatcg aagcagctgt gactggtgat tatgggacag ctttacaggc gtttacgatc
     5281 aatccgctta tacgatcagg aaagacagca aaacggatca tggatgagtt gtttattgca
     5341 caccagcagt atttaccgca attcaaggaa acaatcagcc ggttaaaagc agaaggaatc
     5401 gaagtccaag atgaactggc aagagaatta tcaagcaata cacatgtaat ggaggaagta
     5461 tgatgggatt cagaaaagat tttttatggg gcggagctac agcagccaat caatgcgagg
     5521 gcggttatga tgaaggcgga cgtgggttag cgaacgtaga cgttgtgcca atcggagaag
     5581 accgttttcc tgtcattact ggaaaaaaga aaatgtttga atttgatgaa aatcattttt
     5641 atccagcaaa aattgcgatc gatatgtatc atcattataa ggaagatatt gctttatttg
     5701 cagaaatggg gttcaagact tatcggatgt ccattgcttg gagtcgtatt tttccaatgg
     5761 gcgatgaaaa agagccaaat gaatctgggt tgaaatttta tgaggatgtc tttaaagaat
     5821 gtcaaaagta cgggatcgag ccgcttgtga cgattacaca ttttgattgt ccgatgcatt
     5881 tgatcaaaga atatggtgcg tggcgttcca gagaactcgt tggtttttat gaaaatctgt
     5941 gccgagtcat cttcaatcgc tataaaggac ttgtcaaata ttggttgaca ttcaatgaga
     6001 tcaatatgat ccttcatgca ccattcatgg gcgcagggct gtattttgaa gaaggagaaa
     6061 atgaagaaca aatcaaatat caagcggcac atcatgagtt ggttgcaagt gccatcgcaa
     6121 cgaagattgc tcatgaagta gatccggaga acaaagtagg ctgtatgctg gcagcaggag
     6181 catattatcc atacacttgc aatcctgaag atgtttgggc ttctagaaaa tcagatcgag
     6241 aaaattattt cttcattgat gtccagtcac gaggagaata tcccgcttat gcattgaaag
     6301 aaatggaacg aaatggcatt cagatcgaaa tggaacctgg ggatgccgca ttgctgaaag
     6361 aacatactgt tgattttatc tccttttctt attattcttc tcgagtttcg actactgaca
     6421 acgaactctt ggaacagact gcaggaaata tctttgcttc tgtcaaaaat ccatatctgg
     6481 aagcaagtga atggggttgg caaattgatc cattaggctt gagaataacg atgaatgata
     6541 tttatgatcg ttatcaaaaa ccactattta ttgtagaaaa cggcttaggt gcagtcgata
     6601 cgcccgatga gaatggttat gtggaagatg attatcgaat cgactatctg gcagcacata
     6661 tccaagcaat gaaagatgca gtagaattag atggagtcga tctattaggc tatacgactt
     6721 ggggatgtat tgatttagta tcagctggta caggtgagat gaagaaaagg tacggattca
     6781 tttatgtcga tcgtgacaac gagggaaatg gcacattagc ccgttcgaag aagaaatcat
     6841 tcaactggta taaaaaagta attgattcaa atggagaaaa tctttcgaat gattgatcaa
     6901 tgatttataa aagtctattg tttttcagcg gatttttcga gtataataaa gctattcgag
     6961 aaacgcaagc agaacaatag actttttttc gcattggctg taaagtatgc ttacttgatt
     7021 aagtaaagga gaatctacat atgtatgatg taatcgttat tggcgcaggt cctgctggga
     7081 tgactgctgc tttatatgct tcacgttcaa acttatccgt attgatgatc gaacaaggcg
     7141 cccctggcgg ccaaatgaac aatacggcag aaatcgaaaa ctatcctggt ttcgattcaa
     7201 tcatgggtcc tgatctagct tataagatgt atgaaggtgt ttcaagattt gggacagaaa
     7261 atgcttatgg aatcgtgcaa gatatcaagg atcacggcag ccacaaagaa gttatttgtg
     7321 aagataaaac ttacgaagga aaaacagtca ttatcgcaac aggctgtatc caccgtaaat
     7381 taggcgtacc tggcgaggaa gaatttgctg gacgtggcgt ttcttactgt gcagtttgtg
     7441 atggtgcgtt tttccgtaac aaacgtctag tagtgatcgg aggaggagac tcagcagtag
     7501 aagaagccat ctatttgaca caatttgctt ctgaagtagt catcgtccac cgtagagatg
     7561 aattacgtgc acaaaagatc atccaagacc gtgcatttgc gaatgaaaaa atttccttta
     7621 tgtgggatac agtcgttgac gaaatcgttg ggaatgaaat ggtagtgaca ggtgtgaaag
     7681 cacgtaatgt caaaaccggc aatgagtttg atttggcagc agacggagta ttcatctatg
     7741 ttggactgga tccattaact gcaccattta gagaagcagg gttgacgaac gaagctggct
     7801 ggattccaac agacaacgaa atgaagacga atataccagg cgtttttgcc atcggagatg
     7861 ttcgtgaaaa agatttgcga caaatcacta ctgctgttgg tgatggtggg atcgctggac
     7921 aacaagtgta taaatacttg gaatctttag aagaaacaac tgcataagtt taaaaagagt
     7981 ggaagaacgg tttcaattgt tcttttactc ttttttttcg cgtgaaatct actgaatccg
     8041 atttatgcga atacttgcaa ccgtgcttga taaaagaatc ctttcttgat ttaagcgttt
     8101 tttggtcctc atacttttat atctaataag tgaatgctat acttattaag aaccatattt
     8161 cagaaagagg tgttattttc atgtcttggg aacaagtgta tgaacaatgg gtacatgaag
     8221 aaaatttaga agaaaactta aaaaaacaat taaatgatct gggtgaagat cctgaaaaac
     8281 ttgaagatgc tttttatgca ccgttggaat tcggtactgc aggcatgcgg ggaatcttag
     8341 ggcctgggat caatcgaatg aacatctata cggtaagaca agcaacagaa ggacttgcac
     8401 gtttcatgga tacacaaggc gaagaaacaa aacgccgagg tgtcgctatc gcttatgact
     8461 cacgccatca atcaccagaa tttgccatgg aagcagccaa aacattagca catcacaata
     8521 tcccatctta tgtattcgaa agtctacgtc caacaccaga gctttctttt gcagtccgtc
     8581 atttgaagac gtttgcggga atcatggtaa ctgcatccca caatccagca gcttacaacg
     8641 gatataaagt ctacggcgaa gatgggggac aaatgcctcc tgcagacgca gatgcattga
     8701 cgaaatatgt ccgtgaagtc agcaatccgt taaaagttga agtattatct gacgaagaag
     8761 ctgaacatag cggattgatc acgatcatcg gagaagaagt ggatgccgct tatctagaag
     8821 aaatcaaagc agtaacgatt gatcatgaat tggtggaaac aatggggaaa gatttgaaac
     8881 ttgtgtacac accacttcac ggaacaggaa aaatgttggg cgaacgtgcg ttgaaacaag
     8941 ctggattcga acaattcgta ctagttccag aacaagctgt cgcagaccca gatttcacta
     9001 cagtcaaatc accaaatcca gaagaacact ctgcttttga gtatgcgata cgacttggcg
     9061 agaaagaagg cgctgatttg ctgattgcaa cagatcctga tgcagaccgt ttaggtgcag
     9121 ccgttcgttt gccaaatggc tcctatgaag tattgaccgg aaaccaaatc ggagcactgt
     9181 ttgctcaata tattttggaa gcacaccaat cgcgaggcac tttacctgaa aacgcagcgg
     9241 tattgaaatc gatcgtttct agcgaattac ctacagcaat tgcggaacac tatggggcta
     9301 ccatgttcaa tgtattgaca ggattcaaat tcattgctga aaaaattcaa caatttgaag
     9361 aagatcattc ccatactttt atgttcggat tcgaagaaag ttacggttat ttagttaaac
     9421 ctttcgttcg cgacaaagac gcgatccaag cattgctctt attagcagaa gtagcagctt
     9481 actataaaaa acaaggcaaa acgttgtatg acggcttgca aacgattttt gaaacctatg
     9541 gctactacgc agaaaaaacg atttctgtga caatgagcgg acaagaaggt tctgcgaaaa
     9601 tctctgcttt gatggaaaaa ttccgtgaag aagcaccaac agaatttgcc ggagcaaaag
     9661 tcatccaaac agaagacttc aaagagttga cgagaacaga ccgtgaagga aacgtagaaa
     9721 aactaacgac accgccatct gatgtattga agtatacgct ggaagaaaat ggctggatcg
     9781 ctatccgtcc ttcaggaact gagccgaaaa tcaaatttta catcggtgta aaaggaaact
     9841 cttcagaaga cgcagctgaa aaagtagccg cttatgaagc gattatccaa aagatgacag
     9901 aagaataaaa aaacttgaga ctgatggaaa aagtggtaaa ctttagaagt cagtcttttt
     9961 tcaagttgga ggtgaatgga ttgccagaca cgcaaaaaac aaaagtagag aaagtcagcc
    10021 aactattcaa aatgttgagt gaccccactc gtctcaaaat tctgctgtat ctgaaagacg
    10081 gggaacaaaa tgtgacagca atcacccaag cagtggaaat ggaacaatct gctgtatcgc
    10141 atcaactgcg tttattaaga gaaaaccatg tcgtcaagtc ccatcgtgag ggaaaagcga
    10201 ttttgtatag tcttgatgat tctcacgtac tggatatttt gaaccagaca ctcaaacatg
    10261 tggaacatca ataacgatag gtgcaaatag agacaaactg gactgtgtca acgaatcttc
    10321 ttcgctgaca cagtccagtt ttttttaaga aaaagacaag aaaaactgtt agtctgtttc
    10381 ttcggtggtt tcagttccat aataactatg atcttctagg tctaaagcat tcaaaataag
    10441 cgaagccgtt tcttcaatag ataaagatgc cacattgatc actacacatc ctagtttttc
    10501 ataaagatca ttcgcaaaat cgagttcagc tctaattttt tcgatatcgg aataagcagt
    10561 atcagggttt aatccgtaag agcgcatccg ttctttacgg atattattta aaatatctgg
    10621 gttattcgtt agtcccacga tttttttagg atcgatttcc cacagctgtt tgggaatatg
    10681 tgcttgggga atcaaaggta aattagcaac ttttaaattt ttatttgcta aaaacaagct
    10741 caaaggcgtt ttggaagttc gggatacgcc tagcaaaacg acatctgctt ctaaaaaacc
    10801 acgaggatct ttcccatcat cgtattttac agcaaattcc attgctttga tccgtttgaa
    10861 atagttctca ttcaataaat ggagggcacc aggttgccga attggcttga taccagttaa
    10921 acgttcaatt tctgatatcg gtggcgtcat cacatcgaaa ctaaataatt gatgttcttc
    10981 acaaaaagat cgtgttatct cgatcagttc ttgattgatt aatgtatgca ggacaagtgc
    11041 tttttcttct aatgcatctt gcaatgcctg ccttaatgtt ttttcatctg tcacgaacgt
    11101 tctgcggaat aggttgaact caacggtagg atactgagcc atagtagctt gcgctagttt
    11161 tgtcgcagtt tctccagcag agtcggagat gacaaaaaaa gtaacgattt ctgtagaatc
    11221 ggaagacatg gataaaacct cacttgcatt ttttttatgt ttattatacc atactctaga
    11281 atgcgtactt cgtaatcatt ttgatttgga ggaataagtc atgaaaaaac agacattgat
    11341 cgaacaatca ttagaaacat tgaaaaataa cggttttaaa tatactaaaa aaagagaagc
    11401 attgttaact tatttagtga agcgtaaccg ctacgtatct gctaaagaag tttttgacta
    11461 tatgaatcag gaattcaaag gggtaagtta tgatacgatt tatcggaatc tccatgattt
    11521 tgcagagatc gatttactgg aagaaaccga tttaaatgga gaaatgaagt ttcgttttca
    11581 ttgctgtcag ggggaaatcg gccaccacca tcattttatc tgtacagtgt gcggaacaac
    11641 gaaagaaatc catatgtgtc cgatgaatta tttcgaagaa cagctaggtg gatgcacgat
    11701 cgaagggcat agattcgaga ttttcggaag atgtgaaaat tgtcaataag gatttaaata
    11761 aaataacatt tgataattta aaaaaaaagc aagatttcct tgacggtcaa aacagcatta
    11821 gatataatat ttacaggaca gtcttgtttt ttgagatata tttcaataac aattacggtt
    11881 actttttaag ctcggaggga gggaattaac atgtcaaaaa cagtcgttcg caaaaacgaa
    11941 tcacttgacg atgctcttcg tcgcttcaaa cgttccgtgt caaaagcggg tactttacaa
    12001 gaatctcgta aacgtgaatt ctacgaaaaa ccaagtgtaa gacgcaagaa aaaatctgaa
    12061 gcagctagaa aacgcaaaaa attctagttc tgtgatgaat tggagttggt agtgtgtcac
    12121 tactaagtag attaaacgat gacatgaaag cagcgatgaa agcgaaagat aaggaatctt
    12181 tacaagtcat ccgaatgatc aagtcatcta tacaaaacga gcagattaaa gaaggtcacg
    12241 acttgacaga agaagaagaa ctaacggtgt tgtctcgcga aatgaaacag cgacgtgatt
    12301 ctttgcatga gtttgaagaa gcaggacgtg acgatttagc tgaaaaagta aaaagtgaaa
    12361 ttgtaatagt cgaaaaatat atgccggaac aactttccga cgaagaaatt cgacaacttg
    12421 ttcaagaagc aatcactcaa actggtgctt catcaatgaa agaatttggc aaagttatgg
    12481 gtgcaattat gcctaaagtc aaaggaaaag cagatggaaa ccaagtgaat gcgattgtga
    12541 aagaactatt acaatcttaa ttttatcatt taaactgcgg acagtgattg tccgc
//
LOCUS       DAHHZJ010000078        12549 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.079, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000078 DAHHZJ010000000
VERSION     DAHHZJ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.079"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            136..873
                     /locus_tag="NBL02_001688"
     CDS             136..873
                     /locus_tag="NBL02_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCD2572371.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            887..1702
                     /locus_tag="NBL02_001689"
     CDS             887..1702
                     /locus_tag="NBL02_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCD2572372.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            1715..2362
                     /locus_tag="NBL02_001690"
     CDS             1715..2362
                     /locus_tag="NBL02_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCD2572373.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            2378..3034
                     /locus_tag="NBL02_001691"
     CDS             2378..3034
                     /locus_tag="NBL02_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCD2572374.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            3196..4017
                     /gene="racE"
                     /locus_tag="NBL02_001692"
     CDS             3196..4017
                     /gene="racE"
                     /locus_tag="NBL02_001692"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCD2572375.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            4020..5375
                     /gene="rph"
                     /locus_tag="NBL02_001693"
     CDS             4020..5375
                     /gene="rph"
                     /locus_tag="NBL02_001693"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCD2572376.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            5376..5894
                     /locus_tag="NBL02_001694"
     CDS             5376..5894
                     /locus_tag="NBL02_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCD2572377.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            6001..6501
                     /locus_tag="NBL02_001695"
     CDS             6001..6501
                     /locus_tag="NBL02_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCD2572378.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(6781..7701)
                     /locus_tag="NBL02_001696"
     CDS             complement(6781..7701)
                     /locus_tag="NBL02_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HCD2572379.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            7866..8879
                     /locus_tag="NBL02_001697"
     CDS             7866..8879
                     /locus_tag="NBL02_001697"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HCD2572380.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(8989..9744)
                     /gene="fabI"
                     /locus_tag="NBL02_001698"
     CDS             complement(8989..9744)
                     /gene="fabI"
                     /locus_tag="NBL02_001698"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCD2572381.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            9964..10404
                     /gene="fabZ"
                     /locus_tag="NBL02_001699"
     CDS             9964..10404
                     /gene="fabZ"
                     /locus_tag="NBL02_001699"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCD2572382.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(10519..11280)
                     /locus_tag="NBL02_001700"
     CDS             complement(10519..11280)
                     /locus_tag="NBL02_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCD2572383.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            11469..>12549
                     /locus_tag="NBL02_001701"
     CDS             11469..>12549
                     /locus_tag="NBL02_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCD2572384.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLI"
BASE COUNT         4053 a         2176 c         2643 g         3677 t
ORIGIN      
        1 tagaaatgga agaaatattt atgggagaat aaacgttcta agaaacttta caaattacta
       61 aactatcaga tataatgtat tttattatac atctttaaaa gaaatcttac taaataataa
      121 tgggagtgta attctatggc aatgattaaa tttgatcacg tggataaata ttatggcaag
      181 ttccacgcat taaaaaatat caatctcgaa ttcgagaaag gagaagttgt cgttgtcatt
      241 ggtccttctg gttcaggaaa gagcacgatg cttcgctgta tcaatggatt agagacgatc
      301 tcttcaggta agctgctgat taatgatact gacttgcatg ataaaaagac aaaactgaca
      361 gaagtccgaa aaaacatcgg catggttttc caacatttca atttgtaccc aaataaaact
      421 gttctggaaa acatcaaatt agcaccaatc aaagtgttga agcaagacca ggcaactgca
      481 gtcaaaaacg cggagaaatt tttgaaaact gtcaatatgt tagataagaa agattcttat
      541 ccttccatgt tatccggggg acaacagcaa cgggtggcaa tcgccagagg tttagcgatg
      601 aatcctgaga tgctgctgtt tgatgaacct acttctgctt tggatccgga aatgattggt
      661 gatgtattag acgttatgaa aaaattggct catgacggta tgtcaatgat tgtcgtgaca
      721 catgaaatgg gctttgccaa agaagtggcg gatcgtgtta tcttcatggc tgatggagaa
      781 gtattggaag acagcagaaa tgtgagagac tttttcgaaa atccaaatga agtccgagca
      841 cagcaattta tcagtaaagt tatcaatcat tgataggagg aaaaagatgc gcaggttaaa
      901 aaaaatacgc ctgattttcc ttttaggcat tgcttttctt gcactagccg gatgtaaagg
      961 aaatgggttg gcaaatgaag atattttaga gcgaagcaaa tctaccaatg aaatcatctg
     1021 gggtgtcaag tatgatactc gattatttgg aatgatggat attgagagca gaactgtaca
     1081 aggatttgat gtagatattg caaaagcaat cactaaaaag atccttggtg acaatggaaa
     1141 aacagaattt gttgaagtca cttctaaaac tcggattcca ttattgaaaa acggaaatat
     1201 cgatgcaatt attgcaacga tgacgatcac agatgaacga aaaaaacagg ttgacttttc
     1261 agatgtctat ttcgatgctg gccaagcttt gttggtaaag aaaggaagcc aaatcaaaag
     1321 tgtagatgat ttgaatgctt ctacaacggt tctagcagtc aaaggttcta catcggctgc
     1381 gaatatccga cagcatgcac cagatgcaaa aattttagaa ttagaaaact atgcagaagc
     1441 atttactgct ttacaatctg gtcagggaga cgcgatgact acggataatg cgattttgtt
     1501 agggattgca gatgaaaatc cagagtatga gttagttggt ggaacgttta ctaatgagcc
     1561 ttacgggatt gcgatcaaca aagggcaaga aaacttcttg aaagctgtta accaagcgct
     1621 agaggaaatg catgcagatg gcacatacga taaaatctat caaaaatggt ttccaaatga
     1681 aactgaagga aaagtagagt aagggggaga ggcaatgagc gaattgatac aaacttatgg
     1741 accaatcttt gtcgacgggt tcaagatcac attgttttcg agtgtcttag cacttgtgtt
     1801 tagtttgatt atcggaacgt taatggcaat cttccaatta tcaaagaata aagtagtgag
     1861 cgccttagca aaggcatatg tagaattttt tagaaacatt ccattattaa tcatcgtgat
     1921 gtttttttat gttgtcgtac cgatttattg gtttaatcta gatggattcc aagcaggtac
     1981 gattggatta acgatctata catctgcttt tattgctgaa acagtacgtt cagggattca
     2041 aaccgtacca aaaggtcaaa cagaagcagg actatcttct ggttttacct attcagaaat
     2101 catgcgttat attgttttac cgcaagcatt caaaatcgtt atcccgccat taggcaacca
     2161 atttatcaac ctcgtaaaaa actcatctat tttagcaatc gtagctggtt tagatttgat
     2221 gtatcaagga gacttgatcg ctagtgaaac attcaatacc ttcgatacgt atatcatcgt
     2281 tgggttattt tacttagtta tcacgctacc gttatcttac ttaatggttt atcttgagaa
     2341 aaaatgggcg gttcgttcat agaaggaggg gaaatacatg gattttagcg gcgctttttc
     2401 atggatgaat atccgttttt tattggaagg attaaaggtt actgttgaag tttcattgtt
     2461 ttcgatcatt tttagtttct tgatcggtgg tctgacaggt gtgattcgtt ttgcgaatat
     2521 ccctttcttt tccaaaatcc ttggtgtagt gattgatatt atacggaatc ttccattatt
     2581 attgatcatt ttcttcactt actttgcctt acctcaaatc ggcattcaga tgaacatctt
     2641 ctggtccgct gtcgcagcat taacgatttt tgagtcagct atgttgtcgg aaattttccg
     2701 agctggactc aatgctgttc caaaaggaca gatggaagca ggattatcaa cgggactgac
     2761 ttatgtagaa tcgatgcgaa cgatcgtgtt accgcaagca tttaaatcaa tgatcccggc
     2821 aattgtgagc caattgattt cactgatcaa agatacttca ttagcagtaa tcatttcttt
     2881 acctgaactt tctcatcagg cacgtattat ttatggacag aacaccaatt atgtgcttcc
     2941 gatgttcatc atcatgacac ttatgtactt cgtggtatgt tacgcgcttt ctctgctttc
     3001 cggttacttg gaaaagagac ggtacagtta ttaatagatg attcttttag cggttgtggc
     3061 aaactaaagt cacaaccgct tttcctcttt ccattcgaaa aaaagagatt tcgattgttt
     3121 taagcaagta agagcacttg aaaattcacg ctaaaaagta cacaataggg aggatagaat
     3181 ttattgaaaa aaaggatgat acgattgaca gataatcgcc ctatcggatt tattgattca
     3241 ggtgtcggcg gcttgactgt agtaaaagaa gccctgaaac aattaccgaa tgaaaatatt
     3301 ttatttgtag gagacacagc acgctgccca tatggcccta gacccgcgga acaggtaatc
     3361 cagtatactt gggaaatgac ggattatctg gtggagcaag gaatcaagat gctggtgatc
     3421 gcctgcaata ccgcaactgc ggtggcttta gaagaaatca aagctgctct ttctattcca
     3481 gtcatcggtg tgatccttcc cggtactaga gcggcagtaa aaaaaacaca aaataaacaa
     3541 gttggcatta tcggtacgat tggtacggta aaaagtcaag cttatgaaaa agcactgaaa
     3601 gagaaagtac cagaattgac tgtgacaagt cttgcttgtc ccaaatttgt ttcagttgtc
     3661 gaaagtaatg aataccattc atcggtggcg aaaaaaattg tggcagaaac attagctcct
     3721 ttaaccacta aaaaaatcga tacattgatt ttgggatgca cccattatcc attattacgc
     3781 cccatcattc aaaatgtaat gggagaaaat gttcaactga tcgattctgg agcagaaaca
     3841 gtaggtgaag tatctatgct gttagattat ttcaatttga gcaattcacc gcaaaatggt
     3901 cggacattat gccagtttta tacaactggc tctgccaaac ttttcgagga aatagctgaa
     3961 gactggcttg gaatcggaca cttaaatgta gaacatatcg aattgggagg aaaataagga
     4021 tgcgtcatga cgggagaaaa gcccaagagc ttcgcaaact ggagcttcaa acaaatgttt
     4081 tcaaatatcc agaaggatcg gtagtgatcc gttttggtga tacaacagtt atttgttctg
     4141 caacaataga aaattctgtt cccccttttt tgagagaaac aggaacagga tgggtgacag
     4201 cagaatatag tatgctgcct cgagcaacga gtacgagaaa tcgtagagaa agcagcaaag
     4261 ggaaactttc tggtagaaca atggaaatcc agcgtcttat tggtcgttcc ttgcgtgcag
     4321 tagtagactt ggaaaaatta ggagaacgta gtatcatcgt agactgcgat gtgatccaag
     4381 cggatggcgg tactagaaca gcaagtatca ctggggcatt tgtggcattg aaattagcca
     4441 ttgaaaaact tttaagagaa aaagaattgt cagaagatcc aataaaagaa catcttgctg
     4501 cagttagcgt aggaatctta ccagatggga cttgcgtcac agatttggat taccaagaag
     4561 attccgcagc acttgtagac atgaatcttg taatgacaga atctggaaaa ttcgtggaga
     4621 ttcaaggaac tggagaagaa gcaacgtttg atggtgaaca gttgaatgaa atgctctttt
     4681 ttggaaaaaa cgcgattgaa gatttgatca aagagcaaaa acatgctttg ctaacagagt
     4741 ttgctcaaaa tgatgagcgg atagaagaaa caaaaacaat catcattgcg acaagaaatc
     4801 caggaaaagc agaagagttt cgaaatatgt tcaaagaagc gggatatcat gtaaaaacat
     4861 tacttgacta tcctgaattg ccagatgtgg aagaaactgg cagcacattt gaagaaaatg
     4921 cccgattaaa agcagaaacg attgcacaat tgcttgatca gccagtctta gcagatgatt
     4981 caggtttgaa agtggatgct ttaggcggta tgccaggaat ctattctgca cgttttgcag
     5041 gggaacaaaa aagtgatgcg gggaataacg ccaaattatt atatgaattg acagatgtgc
     5101 cggatgaaaa aagaacagca caattccatt gtacgttagt attcgcggca cccaaaaaag
     5161 acagccttgt agtagaagcg gaatggcccg gtcgagtagc ccgtatacca tctggcgaaa
     5221 atggctttgg gtatgatcct ttatttatcc cagaaggtaa gaaacaaaca gcagcagaat
     5281 tatcgagcga agaaaaaaac aagatcagtc atcgggctca agcaatgaaa aaattgagtg
     5341 ccgagtggaa acaatggctg gaaggagaga gatagatgcg gtatttagta gtaagtgata
     5401 atcatggaga cagagatatc ctcgttgact tagtagatcg ttatcgttct gaagtggata
     5461 tctttttcca ttgtggtgat tcggaattag aagcttctga cccattatgg gatacattcc
     5521 aagtggttca aggtaattgt gattatggac caggattcga acagaaaaaa gtgatccaaa
     5581 ctggtcagga tacagtattc atgactcacg gtcatttatc gaatgtccga tttggtttga
     5641 cacagttagc aatcgaagct cgagctgcaa ctgccgatat ggtgtttttt ggtcataccc
     5701 atcaaatagg ctgtgagatg gatcagaaaa tcctgttttt gaatcctggt agcatttctc
     5761 agcctagagg cccgatccaa atcccatcat atgctcttat cgaaagttta gatgaccaaa
     5821 tttctgttca atactataac cgtgcccatc aggcagttga agagttggcc ttttcttttt
     5881 cgagagagcg ctgaataaaa aaactctaat ttttaataaa aataagcttt attttatgaa
     5941 ttttgcatcc ggtttcggta aaatagtagt gttgaatgaa atagaaatgg aggccatcag
     6001 atgattggac ctatagtaag agaaatgctg ctggaaaatc aggaaacgtt tttgattcct
     6061 gcagaaaatg tggcaaatgt tatgtatcat catccattgt cccatggttt acttgtgtta
     6121 tcgaaggtcg gttattccaa aattcctgta ttaggaaaag atgatcactt tgtaggacta
     6181 gtaagtttat cagatgtggt caataaaatg attgacttgc aaacaatcag catggacccg
     6241 ttagaaggac taacggttgc tgatgtgaca gaaacagatg ttccgacaat tgatgaaaat
     6301 tgggaactag aagaagtact ccatttgtta gtcgattcgc cttttttacc ggttgtcaca
     6361 gaagatcgga ttttcaaagg aatcattaca agaaaagagc tgcttaaagc cgtaaacttc
     6421 atggtgcatg aattagaacg tcgtaatata ttgtctccga aaatcgaaga attaaaagaa
     6481 caaatcgata tcgtgagcta aagaacgaga aactaaaaga taatcaaaaa ataagtagaa
     6541 aaaacagcgt accagttttg actttatctt gaaaaagagg ctgggacaaa agcgtttaac
     6601 tccaagaaat aagaaggaat tcacgaaaat tgcttttcaa atttttgtga atttcagctt
     6661 atttccgaag gagttgcttc tgcttccgcc gtttatacgt gtttagagcg tgggacaaaa
     6721 gtgttcttta cttttgtccc acgctctttt tagtgagatt cgttgcatcg tttgtccaaa
     6781 ttattttccg acagcaattg gactgttcgg aatcgtgaaa ccagaagctg tcaattcttt
     6841 gacataggag ctcaagaact cttcttttaa ggcatattgt tttccgttca atgcgtagca
     6901 gaccgtacga atcgcaaaat tgctgtttcc aagatccact aatccaaaga tagaaggttc
     6961 agtttggagc tcttcttggt atttttcagc taatgtacga tttactcgat caatgatctc
     7021 atagattttg tcatatcctt cttcaggaac aatccgaata tctaaaagta cttgcatatt
     7081 tgcacgagag agattgctga tcgtggtgat gtttcgattc gggatgaaat ggacagtacc
     7141 atccgttgct ttcagctgaa gggtacggat accaacagat acgacggttc cttcaatcgt
     7201 taagtttgct agcttgatgt agtccccaac atccatctgt tgttccatga taataaaaaa
     7261 tccggtaata acatcactca tgaacccttg ggcaccaaga ccgattgcca cacctgcaat
     7321 tccggcacca gccaaaagtg atccgatcgg cacaccgata gcagataaaa ttgcatagat
     7381 aaaaaagaaa ccaagggtat aatgaaaaat agtatttaaa agactatgta aagtctttaa
     7441 gcggctctcg tttagaatgg cttttttaga atactgatta aaagacttat caatcagata
     7501 ttttcccaca cggtgaagaa tccaaaaaag gacgatcaaa aaaatcagat ataaactttt
     7561 ttggataaag gcagccaaaa tggcgtccca atcaatcccg tcccaaaacc gctgaaacgc
     7621 atttactttg ctgacagctt gatctgtcaa tgtctcgcca gaagagtctg tcgtaccggc
     7681 agttgctaat aatgaaaaca taaaatcctc ttttctaaaa tttgattctt tctgtattgt
     7741 accaagaagt tttcaaaaaa ggaaatagca aaaataacta taatgaagga aaaaagctga
     7801 aaataaaatg acaaataata tggataatga taaaatgtgg agaaaataac gaaggtggta
     7861 agtatatgca aatcgattgg aacaatctag gtttttctta tatcaaaact ccttggcgtt
     7921 ttattgccaa atggaaagac ggacaatggg aagaaggaga attgacagaa gataattacc
     7981 tgacgattca tgaaggctct ccagcgcttc attatggtca gcaatgtttt gaaggtctaa
     8041 aagcttatcg tcgaaaagac ggcaaggtca atctctttcg acctgaacaa aatagtcgac
     8101 ggatgaatca aagtgctaga cgattgctga tgccaatggt ccctgaaaag atgttcgttg
     8161 atgctgtcaa gcaagtggta aaagcaaatg aagattttgt gccgccctat ggaacaggcg
     8221 ctacattata cttgcgccct ttactgattg gggtcggtga aaacattggt gtccatccag
     8281 ctccagaata tttatttgtc gtcttctgta cgccagtagg agcgtatttc aaaggtggac
     8341 taaaaccaac gaattttatt gtatctgatt acgatcgtgc tgctcctaag gggactggtg
     8401 cagcaaaagt cggcggaaat tacgcagcga gtttacttcc aggaacagaa gcaaaagaac
     8461 tggattactc agattgtgtt tatcttgatc cagccacaca tacgaaaatc gaagaagtag
     8521 gtgcagcaaa tttctttgga attacaaaag acggaaaatt catcactccg caatcgccct
     8581 ctatcttacc aagtatcacg aaatattcct tgttgcagtt agctgaagaa cgactaggac
     8641 tgcaagcaat tgaaggagat attttcattg atgatctaga tcaatttgca gaagctggtg
     8701 catgtggtac agcggctgtt atttctccaa tcggtgggat ttatcacaat aacaatcttc
     8761 atgtatttta tagtgaaacg gaagtaggac cagtaacaaa acggttgtat gatgaattga
     8821 caggtattca atttggagac atagaagcac cagaaggctg gattgaagtc gtggaataat
     8881 ttcaaagtaa tgagttgaaa aaaacgtaaa aagaaaaaat caggaagaga ctttaaaaag
     8941 ttttttcctg atttttatat gttttacttt aaagaatgca acaaaagttt atgaaagatg
     9001 gacacctttg tcaacgtaga taatatcacc gatgattccg ctggaaagtg ggctgattaa
     9061 gaaagcacaa gcattaccga cttcttcgat cgtcacacct tgctgatcag gcgtacgaga
     9121 ttcagataac tggatcagtt tttgatagtc tttaacacct gtgacagcta atgttttgat
     9181 tgccccagct gagattccat tgacccgaat tcctttaggc gagaactcag ctgccaaata
     9241 acggatagca gcttcaagag aagctttcgc aatacccatc atgttataat tcgggattgc
     9301 gcgttctgaa cctagataag tcatgctaac gatcccgctg tttggtgcaa gaaacggttg
     9361 tgcgtatttt gatacagcaa ttaaagagta gctgctgata tcttgagcta attgatagcc
     9421 gtctctggaa atatcagaca catttccgga caattcttct ttgttggcgt aagcaactgc
     9481 atggacaagg ccgtggatct ccccagcata ttcaccgatg gcattcattg ctttttcgat
     9541 gctttcgtcg ctagaaacat cacattctac agtgaatgct tcttcgccga ccaatttgac
     9601 taatgatttt ttcattcgtt cattttggta agtatagata atttctgccc cttgtttcaa
     9661 cattgcttca gcacaacccc aagcaatgct ttttttgtta gcgacgccca taatgacgat
     9721 tttttttcct tctagaaatg acataatgac tctcctttgt ttctgataaa aatttgatag
     9781 tcgtaaaaaa atagagaata atagctaaaa taacttcttg atttcttaat tattagtcaa
     9841 aatgttttga ttgtcaaact attttttctt aaattaaaaa aagattgcta aatatttagg
     9901 aatatgatac tttgatagtc aaagtaatga aaatggcaat tgcctgaaag aaggagttta
     9961 attatgtctt acgtattatc tgctactgaa gtaatggaat tgattcctaa tcgttatcct
    10021 atttgttata ttgattatgt agatacttta gaaccaggaa aaaagatcgt tgcgacaaaa
    10081 aatgtaacaa tcaatgaaga ttttttccaa ggtcactttc ctgacaatcc agtgatgccg
    10141 ggtgcgttga ttctagaaac actggcacaa gcaggttcga tacttatcct aaaatcagaa
    10201 gagtttctag gaaaaagagc ttatatcggt gggatcaata aggccaaatt ccgtcaaaaa
    10261 gttgttccag gagatgtgct gaaatgtcat tttgaaatca ccaaaatgaa gggatcagtc
    10321 ggtacagcgt tatctgcagc atatgtagat gataaaaaag tctgtgagtg cgagttcacg
    10381 ttcatcgtca accaaaatgc ttgactataa aacctgatat agcaaaacaa aaggaagctt
    10441 acttgctttt tatgtaaaaa gaagacgcct gaatcctagc atacgttgtc atctatgcag
    10501 gattcagacg ttattttttt atttttgttt tgcttcttgc agtatattga ttttatcgat
    10561 ttttacttct tcttttggtt tgtctgaact atctgtttcg atatttgcaa tagcatccac
    10621 gacatccatt ccttcaatca cttggccgaa gacagtataa tggccatcta gtgaaggata
    10681 accgccgttt ttataagcgt cgatgattgc ttgaggatag tcgtctttta aaagaccatc
    10741 atgcatatca tctgtatttt gaacgataaa gaactggctg ccgttactgt taggatcttg
    10801 tgagcgagcc atagataacg cacctcttat attataaagg tggttgttga tctctgtttc
    10861 aaatccttct ccccaaatgc tttcccctcc agtgccatcg cctttaggat cgccaccttg
    10921 gatcatgaaa tctttgatga cgcgatggaa agtcagtccg ttgtagtaac cgtcttttgc
    10981 atgtgtcata aagttttcaa ccgcttttgg tgcgtgttcg ggaaataatt tgattttgat
    11041 tgtgccttta ttcgtcacca tttcgaccag atcttcattt tcggcgacat ctttcgtcaa
    11101 ttgtggtaat tccaatgcgt tcaaatcgac gctactggca gtcgtgcttt ccgcagtaga
    11161 tgtacttttt gaactttctg tagacgaact agaagaagtc gtgttgtttg cagaacaacc
    11221 agcagcaaac agcaatgctg ctgctaaact aatacttaaa ataactttat ttttcttcat
    11281 aactgttcct ttccaattta ctattttgtt atctttaatg ataacaaaat agagggaaaa
    11341 tacctactta aattattgga atttatcatt tattttaaaa gaaaaagaaa aggtgtagac
    11401 catttacttt gtgtttgcta taatgtaagt ggatacaaaa acgaaaagat gcaggaggaa
    11461 atggtaatat gggaaaatta ggaatctcga tttatccaga acggtcaaca tttgaaaagg
    11521 acaaagcgta cttggattta gctcataagt atgggtataa acgggttttt accagtttgc
    11581 tgcagatcaa tgatgataaa gaaaaagttc tttcagaatt taaagaagta gttgattacg
    11641 cgaatagcct aggcatggaa gtaatggttg atatcaatcc agcgttattt gaacagttgg
    11701 agatttctta cgatgactta tcttttttcc ataagatggg tgcctacggg gtccgcttag
    11761 atatcggatt cacaggagca gaagaagcaa aaatgacgcg caatcctttt ggtatcaaaa
    11821 tcgagatcaa tatgagttcg ggcacgaatt acgttgacaa catcatgagt tattcgccaa
    11881 acacggataa tttattaggt tctcataatt tttatcctca tcgatactct ggtttaggat
    11941 acgaacactt tgttttttgt tcagaaaaat tcagaaaata taatttgaat acgatggcat
    12001 tcgtcaattc gcaatctgct gagtttggcc cttggcctac gcaagatggc ttgtgcacgc
    12061 tggaagacca ccgagatctt gaaattgcga cacaagtgaa gcatttaatc cttacaggat
    12121 tgattgatga tatttctatt ggaaatgcgt acgcaagtga agaagaattg aaagaaatgg
    12181 cagaagcatt taacgcggat tatccaacat tgaaagtaga tacggaagaa ggtattacag
    12241 aaaacgaacg aatctgcttg tttgataacc ttcatagtta tcgaggggat cgctcagaat
    12301 atatccttcg ttcaacgatg actcgtgtat actacaaaga taaagatttc ccgcctcata
    12361 atacgcgaga tatgcatcat ggggatgtac tgattgacaa tgaagggtac ggtcaataca
    12421 aaggagaaac acaaatcgcc ttgaaggata tgaaaaacga cggacgtgtc aatgttgtag
    12481 gaagaatctc cgatgacgaa ttattcctcc ttgacttttt gaaaccatgg tcaagcttta
    12541 aattaattg
//
LOCUS       DAHHZJ010000079        12233 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.080, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000079 DAHHZJ010000000
VERSION     DAHHZJ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.080"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            12..161
                     /locus_tag="NBL02_001702"
     CDS             12..161
                     /locus_tag="NBL02_001702"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572385.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            complement(195..359)
                     /locus_tag="NBL02_001703"
     CDS             complement(195..359)
                     /locus_tag="NBL02_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572386.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            654..1982
                     /locus_tag="NBL02_001704"
     CDS             654..1982
                     /locus_tag="NBL02_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HCD2572387.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2184..3017
                     /locus_tag="NBL02_001705"
     CDS             2184..3017
                     /locus_tag="NBL02_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCD2572388.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            3033..3770
                     /locus_tag="NBL02_001706"
     CDS             3033..3770
                     /locus_tag="NBL02_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HCD2572389.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3925..4893)
                     /locus_tag="NBL02_001707"
     CDS             complement(3925..4893)
                     /locus_tag="NBL02_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCD2572390.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5125..5958
                     /locus_tag="NBL02_001708"
     CDS             5125..5958
                     /locus_tag="NBL02_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCD2572391.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5992..6831
                     /locus_tag="NBL02_001709"
     CDS             5992..6831
                     /locus_tag="NBL02_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCD2572392.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6857..7417
                     /locus_tag="NBL02_001710"
     CDS             6857..7417
                     /locus_tag="NBL02_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCD2572393.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7616..9337
                     /locus_tag="NBL02_001711"
     CDS             7616..9337
                     /locus_tag="NBL02_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HCD2572394.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9502..10644
                     /locus_tag="NBL02_001712"
     CDS             9502..10644
                     /locus_tag="NBL02_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCD2572395.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10660..11871
                     /gene="thiI"
                     /locus_tag="NBL02_001713"
     CDS             10660..11871
                     /gene="thiI"
                     /locus_tag="NBL02_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCD2572396.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4163 a         2053 c         2545 g         3472 t
ORIGIN      
        1 ggtagtagcg catgactgtt aatcatgatg tcgtaggttc gagtcctact gccggagtaa
       61 ataaagctta tgcaaagaaa aagaaatcca atcacttggg tttctttttc ttttattatt
      121 atgatgaatc aagctttctg tttttgtcac gtatgttgta atctttacga aagagtagag
      181 ttggctactc ataatcaacc atacttccca tcatcgctaa atacgaaata aatcagcatt
      241 cctacaccaa tcaaagcaca agctgcacca agaaaaggga tgacattccc aaagttatct
      301 gtattcataa gtatgccacc acccacaaac aaaaagccgc cagcaatcag gtttttcatt
      361 ttattcatct cctttcttac gttatagtat ttccttcttt caatgagtat tttgaaagct
      421 atcttattgt atgatgttct tctaggcctc ttctttatta tacaggccat tttcgttatc
      481 tcatagaaat attccttttt atctgaaaac tattgtatac cgtatgacat ttttcttcat
      541 gaagttgtaa gtatattctc attcaaatga aaatttcatc atagaagaga ttgtatttct
      601 aaattcttaa gagtaaaata cgaagtataa cttggatttg gaaggatgat tggatgaaag
      661 ctattacgcc tgatatgaca acaaaattta acaaacagtt tcaagcaaat atcaaacagc
      721 aggcattgca gcgggcagtt gtgaagaacg gaattactgc ttctgctgaa aatgtttcag
      781 cacatgtgga gaatacaccg gtattttcta tagatttggc tacggggaaa gtggctaatc
      841 aaatgcaatc ggggcgttgc tggatgtttg ctgcactgaa cacattccgt cataagatgc
      901 tgaatcaatt ccatttaaaa gagtttgaat tatcacaaaa ttacactttt ttctgggata
      961 aatatgaaaa ggcaaattat ttctatgaaa acatcattgc aacaggaaaa gaggcaatcg
     1021 atagccggaa agtagctttc ttactcgcta ctcctcaaca agatggcggc caatgggata
     1081 tgatcgtttc attattccaa aaatatggcg ttgtgcctaa atcggtgatg ccggaaagtg
     1141 cgaatagttc aaattctcga gatttgaata actacttgaa caaattactg cgtaaagatg
     1201 cagtagtgtt acggaaaatg gttgctcaag gagaaacatt agacgctatt gaagaaaaga
     1261 aagaagaaat gctagaaagc atttataact tcttagctat ctcattagga acacctccaa
     1321 aagaattcga ttttgagtac cgcgatgaag aaaagaacta tcatcttgac cgaggactga
     1381 caccacaaat tttttatgat aaatacatcg gtgtaaaatt agatgattat gttagcgtga
     1441 tcaatgcacc aacaaaagac aaaccattca atcgttctta tacagtagag atgctaggaa
     1501 atgtcgttgg cgggaaagaa gtcaaatatc tgaatgttga tatggaaact ttcaaaaaat
     1561 tagcgattgc tcagcttgaa gaaggagaat ctgtttggtt tggttgtgat gtaggtcaat
     1621 cttctactag aactagtggc atcatggcct tagatgctta ttcaatggat gatctatttg
     1681 atacggattt tacaatgact aaagctgaac gtttagattt tggagaaagt ctgatgaccc
     1741 atgcgatggt attgaccggt gttgacttag tagacgggca atcaacaaaa tggaaagtcg
     1801 aaaatagctg gggcgaaaaa gttggaaaga atggcttctt tgttatgagt gatgcctgga
     1861 tggacgaata tacgtaccaa atcgttgtaa gaaaagattt attgacgaaa gagcaattaa
     1921 atgcgttcaa tgaagaacca attgttctat ctccgtggga tccaatgggt gctttagcat
     1981 agacgagtat ttcaatgaag agtatgtagt aaagataaaa agagctggct taagccagct
     2041 ctttttatct ttaaagtcca ttccctgttt tttatggtcc ctgtgctata attctaaata
     2101 aaggataagg attggaaata atccttttat cattaaaaat atgatgacga agtagaataa
     2161 aattgggagt aggggggaaa gtcatggata tccaaactgt tttatctgat ctgaaattaa
     2221 atggtaaagt gattccagtt gttggtggcg atgttaatca gacctatcgt ataaaaaccg
     2281 aacatcgtgc ttatttttta aaaatccatc cgaatgtaaa aaaaggattt tttgaagcag
     2341 aagtagacgg attaaaagaa ctgtcagcat tcgtacgtgt accggatacg tatatgctag
     2401 gtgaaacaag tgaaggcgct tatttgttaa tggaatggat cgagccggga aaaggggatc
     2461 aacgagatct tgctgcagct ttagctaatc tccatcagca aacagcgcca caatttggtt
     2521 ttcgtaaaga taactatttg ggtacacttg ttcagaaaaa ttcttttgaa gaagattggt
     2581 ggactttttt ctttaaagat cggcttgaat cccagatttc tttggcagaa gaaacgaatc
     2641 gatggaatgt acagcgtcaa gaaaagtatc tgcgatttaa agaacgtgta ttgaaatcgg
     2701 ttgaaccaaa aaagatcact ccacgcctct tgcacggaga cctgtggagt ggtaatgtgt
     2761 tctttgacca gcaagggcat ccagtttttg tcgacccagc tgtttcttat gggaatcggg
     2821 aacaagatat tgcaatgagc cagttattcg gtggtttccg accggaattt ttagatgctt
     2881 atcaaactat atttccatta gaaaagggat ggaaagaccg actgccgatt taccagttat
     2941 attatctttt ggcacatttg aatatgtttg gagaaagtta cggttcccaa gtagatcaat
     3001 tactagaaaa tttttaagag gagggcgtgt ttttggcaaa aaaatatacc aaaaagcatg
     3061 aagtttcata ttatgagtgt gacatcaacc aaacgatgac atttccttcg atgttaggag
     3121 tagttatcaa aacgtctgaa gatcaaagcg accagttggg aagaggcagt gaattcgtca
     3181 atagctttgg acttacttgg gtcatcacga attattccat ggagattaat cgtctcccta
     3241 gagtcggtga agtaatctca gtaacgactc aagccatgga gtacaataag tttttctgtt
     3301 atcggaactt ctggatccat gatgaagatg ggaaagaatt agtcaaaata gaatctgttt
     3361 tcgtattgat ggattttgtg aatcgaaaaa tgagcagtgt taatgaagag atcattgctc
     3421 cttttgagag tgagaagatc aagaaaatca agcgtcagga aaaaattgaa aagattgatt
     3481 cgggcttcat gttgccatat cgtgttcgtt tttatgatat cgatagtaat cagcatgtta
     3541 ataatgcgat gtattttaat tggatcattg acgtattagg atatgacttt ttgactacgc
     3601 atgttccaga aaaagtaatt attcgatttg ataaagaagt agagtacggg aatgagatcg
     3661 aaagccattt tgaacaaatc cctatcgaaa atggtgtcaa aacaaaacat gaaatccgaa
     3721 tgcaagaaca gctgtattgt gaagcaaata ttatatggaa aaataaataa aaaagaggtt
     3781 gtgacagaag ggggcaactt caagaaataa gaagaaattt acgaaaattg tttctcaaat
     3841 ttttgtaaat tttcgactta gttttcgaag aagctatttt tgtcacagcc tctcttttta
     3901 agtttagcta ttagataatg atgtttagtt ttggataaac gaaatcaatt cttcacccga
     3961 caaaccatag tttacagcaa tcaatagttt taaacgtgct ttttgactat taatactgtt
     4021 acagaaaata atccccattt tttctagttc ttttcccccg ccttcataat cataaacagg
     4081 ctctgcgatt ccattgaaac aacgagaaac aagtactaca gggatttttt ggacaagaag
     4141 cttttccaaa gcagctaagg cttgtggagg gagattcccc gcacctaaag cttcgattac
     4201 caaaccgtct actttcgtat gggcgatggc ttccaataag tccccttgca tcccagcata
     4261 tgctttaacg atcgggatag tcccatctac agcggaaatg tccagtcgct ttgtttctaa
     4321 aagttcctgc aagaataaaa ttcgattctt agttactaag ccgatgggac ccaatgtagg
     4381 tgttcggaaa gtagcaacat ttgtggtatg ggtctttgtc acgtagcgtg cagaatggat
     4441 ctcatcgttc atgaccacca aaaccccctt atctagcgct tcttcacaac ttgcgacacg
     4501 aatcgcactt tcaaaattat aaaggccgtc acttccaagt tcattacttg aacgcattgc
     4561 cccagtcaaa acaattggta atttgttgcc aatcgttgta tcaagaaaaa aagcagtttc
     4621 ttctaatgta tctgtaccgt gtgtgatcac cactccggat atacctgatg cataagcgga
     4681 caagatacgt tcttttaata ccaacatgtg agaaggcatg atgtgtggtg agggaagatt
     4741 gaagatatct tcaactaata attctacatg cttggggatt gtgatttctg catctaataa
     4801 tgggtttgcc acatcaggac taacaccgcc cgtgagcatg ttttctttca tggcgatcgt
     4861 tccgccggtg tgtagtacaa gtattttttt cattttacag acttccttac gaataatttt
     4921 gcatagccgt taaatcttat tgtatactat ataaaagagt tttgctatca gtgaagaata
     4981 acgaatagcc taacttatca taaaaaagct gaccttatta aaatggaaaa gactcaacct
     5041 tgacctccta atacaatgga gtatctggtt gagtttcttt tacaagaaag cagccaagga
     5101 aatcaatcac gaaggtggcc ggaaatgatc agaatcggat taacatcgtt taaagaacat
     5161 gacaaattaa ctggtaaaaa gaataacact ctttatgaat atgcagggtt tctaccctta
     5221 gtggaattag atactgccta ttatggaatt ccccgaaagt cctcagttga gaattggata
     5281 agtgaagtac ctgaatcatt tcgctttgta gtcaaagtct atcaaggaat cagcggtcaa
     5341 ggagactgga agccttatta tcagaccgaa gaagagatga ttgcgcattt tttagatgtt
     5401 atgtcgccaa tggtagatgc tggaaagatg ttttgctttt tagtccaatt cccctcatca
     5461 ttcaaatgta cgaaagaaaa tgtccagtac ttgcgcaaac ttagaaagtg gtttgagggt
     5521 tatcctgttt ctatagaatt gcgggattat tcttggtaca gcgatgaatt tcttgaacgt
     5581 acaagaaact ttatgtctag agccgctttt tctttagcca tcatcgatga gccacagttg
     5641 ctgaatacaa cgattccttt tgatccattt gtcaccaatc cgaactttgc tctttttcga
     5701 ttccatggta ggaatgcgca aggatggcaa gcaaatgata aagagtggcg taaaaaaaga
     5761 acgttatatc gttattcaga aaaagaactg caagaactag cacatgaagt caaagtagtt
     5821 gattctaaga caaaagaggt aggtgtgatt tttaataata attctggtgg tgatgcggct
     5881 gaaaatgcat tagcactgca aaaaatatta aatatcaagc cagatggact caatccaacc
     5941 caaatggatc tgttttaagg gcttatttta ttcaagaaag gggcgaagtc tatgatcaaa
     6001 gctatttttt ttgatattga cggaacattg gtcaccaatc gttcaaaagc gttggaatca
     6061 acaaaacaag caattgaata tgctcggaaa aacggcattt tatgcggtgt agcaacgggg
     6121 cgcagcccag taaagatcaa agaaatcatt gatgaattgg aattagatat gtatgtcgtg
     6181 tataatggtc aactggtttt tactgcagat cgcacgatta tcgaccaccc ttttgaacaa
     6241 aaagtattgg aacatattgt tgaatttgca gatgagaacc atcgacaaat cgtttttggt
     6301 gcgagaaatc ggttagatgg atcaacgacg atgctattag gacaatcgat ttttatcaag
     6361 cgactagtca gctttttacc gcgtaaattc ccggtacgtc tgatgaaaaa aatcctccag
     6421 gtttttagcc ctcaccgaca aaaggatcgt tatgaaacgt tagagatatt aaaagaacca
     6481 atttatcaat gtatattgct cagtccggag tcagaacaga aaaagttaga acatcgcttt
     6541 cccgagtgta cgtttcagcg ttcaaatacg tatactgtag atatcatccc aaaaggtggt
     6601 tcaaaattac taggcatcca agctttcgca aatgctgtcg aaattgaaat ggaagaaatt
     6661 atggcattcg gcgatcatta caatgatatc gagatgctga aaggtgtagg aattggagtg
     6721 gccatgggga atgctcagat cgaagtaaaa catgctgcag actttgtgac tcaatcaaat
     6781 gaagaagatg ggatatacga aggactgaaa cacttcggtc tgatttatta gtcaatgaaa
     6841 gaaggaaaaa atcagtatgg aagcagagac accttttcaa aaagtagaaa cattccacaa
     6901 aacttttgat ccaagaagac cagagatacc cacggctttt acagaaaatg aagctcatac
     6961 acgtgctgcg ttcaaaatag aagaacttgt tgaatttgtt gcagctactt cacctaataa
     7021 agaacaactg catgaagctg ttatgctgct gcacgaagca ttagataaag cagaagaaaa
     7081 aatacttggt aaaacacagt ggggaagtga tccgctggtt gaacaagtag atgcactgac
     7141 agatatcctt tactttactt atggttcttt ttcattacta ggaatagtgc ctgatcgaat
     7201 attcgaaatc gtccatcagg caaatatggg gaaattattt ccagatggaa aacctcatta
     7261 tgaccctgta acaaataaag tgttgaaacc tgaaaactgg cagatggaat atgctccaga
     7321 acctaaaatc gcggcagaaa tccaacgtca gattcaagaa gcgattgcta agaagaaatc
     7381 cgctgatata ggcatgtgca caaatgaaaa agagtgatca gagtttcact atttttgtta
     7441 tctttatacg atggaaaaac aaaagatttt cgtttaagtt agtgaatgca tgaaaatagt
     7501 atgctataat ctagaataga tgtcttttgc acgagagacg tattatttgg atttatgcca
     7561 agcagaacgc catctgttgc ataaaagatg catactgaaa acggaggaac cataaatggg
     7621 aaataatgtt atcatcggaa tcgtcattgc aatcattata gtcgccattg tcgtcttttt
     7681 ggttagttac ttgatgaaga aaaaaaacca agatcgattg gatgccttag aaaaacgcaa
     7741 ggaagaatta tttgacttgc cagtgattga agaagtcgat gatgttaaaa aaatgcatct
     7801 ggttgggcaa agccaaaata cctttcgtga atggagtcaa aaatggaatg acatatctac
     7861 ttcctcgttc gctgaactag aaagtcaaat tttcgaagtg gaaaacctca atgaaagttt
     7921 tcggttctta aaaggaaaaa aagcagttgt tgaagcagaa gctacaatgg accaaatgga
     7981 gaaacaagtg acggaaatcc gtgaaggtct aaaggaattg cgggaaacgg aagaacgcaa
     8041 ctcactagct gttcagcagg cacttgacgt ctatgaagaa ctgaaagaaa aagtcagaga
     8101 agacgagcat gaatacggac cagctctttt tgaattgcaa aaacagatca aagtgatcga
     8161 aaaagaattt acgcagtttg tgactttaaa cacttctgga gaccctgttg aagcccgcga
     8221 ggttttggat aaagcagaaa aacatacgta tgaagtggaa gctctgatga aaaaaattcc
     8281 accgctgtat gaggagctcc ataccacttt ccctgaacaa cttgaagaaa tcagtgatac
     8341 ttacgagaaa ttgatggaag aacaatatgt attcccagaa gaagatcttg ctgaagatat
     8401 cgcaaaagta agtcgaagaa ttgaaaattc attagccaat cttgaaaaaa cagaagttga
     8461 aacggtcgaa tttgaaaacc gtgaaacagc tgatttgatt gattcgttat atgatatctt
     8521 agaacgggaa atggaagctc agagatatgt aaaaactaac caatcaacga ttgcagagta
     8581 tatcaaacat acaacaaaga ataaccgtca attattgatt gagctggatc acacatctca
     8641 aagctataca ttgaaccaca atgaaattgg ccgtgtacga ggtttccaaa ccgaagtgga
     8701 agaaatggaa cgtcaaaatg aacagatgat tccacagatc cgtcaacacg aaattcctta
     8761 ttcagaagtg cgcacgttct ataaaacagt cttcaaagtc ttagaagata tcgaaaagca
     8821 acaagtagag atcgacgact cattacatga attgagaaaa ggcgaaaaag aagcgcaaga
     8881 aaagatcgat acattcgaat tcaaattgcg cagtttgaaa cgttttgtag aaaaacaacg
     8941 tttgcctggc ttgccgaatg attacctaga attctttttt gttgctactg accgtattga
     9001 agaactaagt gttgtcttga ataaaattcg agtaaacatg gaagaggtca atcgtttagt
     9061 tgctctatgt gaagaagatc tagaattatt agataagaag acacatgatt tagtagatgc
     9121 agcagcatta acggagcaaa tgctccaata tgcaaatcgt tatcgtcata cacacgaaga
     9181 agtgagagaa gcaattgata aaagctacta tttattcaat aaagaatatc actatcaaga
     9241 agcattagac gaaatcggta cagcattaga gcgagtagaa cctggtgctt tcaaacgcat
     9301 cgaagatttt tattttaatc atccagactt agtataaagc aaaaaagtta gtaaaccagc
     9361 aagagcaaga cggattattg tcttgttctt gtcttttttt gtatatttga aaattgaaac
     9421 aagtgaaaat ttcacctgct cttgaaagca ccttggacaa ccgttataat agaaaagaat
     9481 ttcaaaaaag aggggtttct tatgatatat tttgataaca gtgcaacaac cccaatcttt
     9541 ccgcaaagct tagatgcata tgtaaaaacc agtcagcgga ttattgggaa tccatcaagc
     9601 ttgcatgatt taggcaatca agctaaccgg ttgttgcagc aagcaagaaa gcagatcgct
     9661 gatttgatcc atgtagaacc agaagaaatc tattttactt caggcggaac agaaggagat
     9721 aactgggtat taaaaggaac gatgatcgaa aaaagagaat atgggaatca tatgatcatt
     9781 tcaggagtcg aacatccggc cgtcagcgag acagcagaac aattaaaaga attaggaatc
     9841 gaagtaagta tcgccccagt ggataaacgc ggatttgtac gtgtcgacga attgaaagaa
     9901 ttaataagaa aagagacagt attagtctct gttatggctg tgaataatga agtaggatcg
     9961 attcagccca tccaggaaat cagtgatctt ttggatgatt ttcctaaaat tcatttccat
    10021 gtagatgctg ttcaggcgat tggaaaagta ccattctcgc aatggttgac agaaagagtt
    10081 gattttgcaa cattttcagc tcataaattc catggcccac ggggaactgg tttcatttat
    10141 tggaaaaagg gtcgtcgttt agcaccatta ctgacaggtg gagggcaaga aagaaatcag
    10201 cgaagtggga cagaaaatgt tccaggtatt gtggctatgg ttaaagcttt acgtttatca
    10261 ttagagataa aggaacaaaa acctgagcag acacaacgct taaaagtagc tctaatcaat
    10321 gcgttgaaga cgtatgagaa agtgacgatt ttttcagaag gaactgacta tgcaccgcat
    10381 attttatgtt ttgctctaaa aggaattcgc ggcgaagttc tcgtccatgc cttagaagaa
    10441 aaacaaatat ttgtttctac tactagtgcc tgctcctcac gtaaacatat ggcaagcagt
    10501 acattgcacg caatgcatgt accaaatgat ctggctacat ctgctgttcg aatcagctta
    10561 gatgaaaaca attcgcttgc agaagtcgag caatttatga ttattttcaa gcagctttat
    10621 aagaaatttt ctgcaatcaa ttaaaagagg agtgttcttg tgaaatacag tgaaattatg
    10681 gtccgttatg gggaactttc gacaaaagga aagaatcgaa aaagctttat catgcaacta
    10741 gcacaaaatg tcagacaagc tttgaaagac tttccagaaa tcaaaatcca tgctgatcgg
    10801 gaccggatgc atcttttatt gaatggcgca gacgggcagc tagttattcc aaaactagca
    10861 aaaatatttg ggatccaaaa tttctctcca agtattcgtg tagaaaaaga tgtcgaagtt
    10921 ttgaaacaag ctgttcaaga catcatgaaa gaaatttata ctgggaaaga aacatttaaa
    10981 attatcgcta aaagaagcga tcaccaattt gaattgacaa gtaatgaatt gaatcaaaca
    11041 ttaggtaatg ctgtttttga tattttcccg catatccaag ttcaaatgcg tcaaccagat
    11101 attccattac gtgtagagat tcggcgtgat ggcgcttatc tatcgtatga aacgatcaag
    11161 ggagctggag gattgcccgt aggaacaagc ggaaaaggaa tgcttatgct ttctgggggg
    11221 attgattctc ctgtagcagg gtactttgcc atgaagcggg gagtggaaat tgaagctgtt
    11281 cattttgcta gtccgccgta tacaagtgaa caagcactgc aaaaagcaaa agatctaaca
    11341 gccaaactag caccttatgt gggaacgatc cattttatcg aagtgccatt taccgagatt
    11401 caagaagaaa tcaaaaagtc agtgccacaa ggatattgga tgacaattac tcgtcgaatg
    11461 atgttgcgtt tgaccgatga aattcgccgt attcgtcatg gtttagtcat tttgaatggt
    11521 gaatcattag gtcaagtggc ttcacaaact ttgcaaagta tggtggctat caatgaagta
    11581 acgaatacgc caatcattcg gcctgtagcg acaatggata aacttgaaat cattgaagta
    11641 gcagaacaga tcgatacatt taatctttct atccagcctt ttgaagattg ctgtacgatt
    11701 tttgctcctc cacagccaaa aacacgccct caactggaaa aggtccatca atatgaagag
    11761 cgtttagcaa tagatgaaat gatccaacga gcactagctg gcttgaagat ggaagagata
    11821 caagcagaat cgtctaatga aaaaactgaa gaattttcag aattgctata agaggttatg
    11881 aaaatagtgt tttgaccaga gtattagaga aacaaaacga aaaaatagct ccatgtattt
    11941 taccgttttt gaactaatgt cgaagattag ctgattgaat accatttatc aagaagttat
    12001 ggaaaagccg gttctttttt actaactgaa aaataaaatt ttatttagag atcaagctac
    12061 agttgataaa tgatataaaa aagagaattt cccatgaaat agcgaggttg tgacagaagt
    12121 gggcagcttc aagaaataag aaggaattta cgaaaattgc ttttcaaatt tttgtgaaat
    12181 ttcggcttat ttttcgaaga agttacttct ggaacaccgt gtatcagagg ttg
//
LOCUS       DAHHZJ010000080        12133 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.081, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000080 DAHHZJ010000000
VERSION     DAHHZJ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.081"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(15..572)
                     /gene="frr"
                     /locus_tag="NBL02_001714"
     CDS             complement(15..572)
                     /gene="frr"
                     /locus_tag="NBL02_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCD2572397.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(575..1297)
                     /locus_tag="NBL02_001715"
     CDS             complement(575..1297)
                     /locus_tag="NBL02_001715"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCD2572398.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1433..2314)
                     /locus_tag="NBL02_001716"
     CDS             complement(1433..2314)
                     /locus_tag="NBL02_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCD2572399.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2413..3195)
                     /gene="rpsB"
                     /locus_tag="NBL02_001717"
     CDS             complement(2413..3195)
                     /gene="rpsB"
                     /locus_tag="NBL02_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCD2572400.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3554..4033
                     /locus_tag="NBL02_001718"
     CDS             3554..4033
                     /locus_tag="NBL02_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HCD2572401.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4250..5341
                     /gene="phnW"
                     /locus_tag="NBL02_001719"
     CDS             4250..5341
                     /gene="phnW"
                     /locus_tag="NBL02_001719"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HCD2572402.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5334..5462
                     /locus_tag="NBL02_001720"
     CDS             5334..5462
                     /locus_tag="NBL02_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HCD2572403.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5466..6011
                     /locus_tag="NBL02_001721"
     CDS             5466..6011
                     /locus_tag="NBL02_001721"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HCD2572404.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6465..8156)
                     /locus_tag="NBL02_001722"
     CDS             complement(6465..8156)
                     /locus_tag="NBL02_001722"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCD2572405.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8576..9523)
                     /gene="arcC"
                     /locus_tag="NBL02_001723"
     CDS             complement(8576..9523)
                     /gene="arcC"
                     /locus_tag="NBL02_001723"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCD2572406.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9638..10657)
                     /gene="argF"
                     /locus_tag="NBL02_001724"
     CDS             complement(9638..10657)
                     /gene="argF"
                     /locus_tag="NBL02_001724"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCD2572407.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10748..11977)
                     /gene="arcA"
                     /locus_tag="NBL02_001725"
     CDS             complement(10748..11977)
                     /gene="arcA"
                     /locus_tag="NBL02_001725"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HCD2572408.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3314 a         2519 c         2194 g         4106 t
ORIGIN      
        1 tttttatgat tttattaaac ttccaataat tctttttctt tatcagcagc gatcttatct
       61 acttctttga tgctgttatc tgtcaatgtt tgtacgtctt tttctaatcc gcgcaaatca
      121 tcttcggtaa tatcaccatt tttttgttgt tttttatatt catcgatcgc atcacgacgg
      181 atgttacgaa tcgcgatttt tgcattttct gcttcttttt tcacatcttt agctaattct
      241 tttctgcgtt cttctgtcag ttgcgggata actaagcgaa tgacatttcc atcattggtt
      301 ggactgattc cgatatcact tgccataata gctttttcaa catcttgaag aatgctcttg
      361 tcaaaaggtg tgatcattaa aacacgtgct tctggaattt ggattgatgc catttggttc
      421 aatggtgttg gcgcaccgta gtagttcaca gtaatacggt ccaaaaggct agcgttcgca
      481 cggcctgcgc ggatttgccc aagttcacgt tgaagatttt gtgctgcttt tttcattttt
      541 tcttttgctt ctgtcataat tgcatctgcc atttttattt cccccttaca gttgttccga
      601 tattttcgcc taagattgca cggcgaatat ttccgtgttc atttaaattg aagacaagca
      661 gtggaatatc attatccatg cttagtgaac ttgcagttga atccatgact tgtaatcctt
      721 tggcaataac ttccagatga gtcagttctt caaattttac tgcatttgca tctactttcg
      781 gatcagcaga ataaacaccg tctacattgt ttttagccat caggataaca tctgcgccga
      841 tttctgccgc gcgtaatgct gctgttgtgt ctgtcgagaa gtacgggttg ccggtaccgc
      901 ctgcaaagat aacgatccgt cctttttcta aatgacgttc tgctttacgg cgaatatatg
      961 gctctgcgat ttgacgcatt tcaatcgaag tttgcacgcg tgtcggtaca cccacgtttt
     1021 ccaacgtatc ctgtaaagct aaggcattca taactgtagc taacataccc atgtaatcag
     1081 cttgagcacg ttccatgccc atttgtgctc cgatttgtcc gcgccagata tttccgccgc
     1141 ccacaacgat tgccatttca acgcccagct cgtgaacttc tttgatttct tggatgattt
     1201 ctttgattac tggtggtttg attccaaatc ctgcgtcccc agctaaagct tctccactca
     1261 attttaagac tacacgctga tatttcggtg tgaccatgtt cattcctcca atattttcta
     1321 ttgccatttt accatactta gatgatttcg acactctaag actggttttt cttgttttgc
     1381 ttaaaaaaag ggagcgcaca aaccgtgcgt tcccgtctgt tattcaacag aattattttt
     1441 tcacttggct cataacttct tctacaaagt tatcttcgcg tttttcgata ccttcgccaa
     1501 cttcgaaacg tacgaatgtt ttaacagttg cgccttttga tgcaacgtat ttttcaacag
     1561 tcatatcagg atctttaacg aatggttggt ccaccaaagc gatttctgct ttgaatttct
     1621 tcaagcgtcc ttctaccatt ttttcaacga tattcgctgg tttgccttcg ttcaatgctt
     1681 gttctgtaag aacagttttt tcatgttcta attcagcttc tggaatttgt gattcgttta
     1741 cataacgagg attgatagct gctacgtgca tagctacgtc tcttgcaact gtttcgtcag
     1801 ttgttccatc tagaacagtc aatacggcaa tacgtccgcc catatgcaag tatccaccaa
     1861 atgcagcatt gtcgtctttt tctacaactt caaagcgacg gaagctgatt ttttctccga
     1921 taacttgagt tgcttcgatc aagtcagatt caattgtacc tttatctgtt ttgattttca
     1981 ttgcagcttc catgtctgct ggtttgtttt cagcaacaag ttctgcaatt tcttttacta
     2041 aatcttggaa catttcgttt ttagaaacaa agtctgtttc tgagtttact tcaacgattg
     2101 ctgcagtgtt acctttcaca gcaactgaag ccaatccttc agctgcaatg cggtcatttt
     2161 tcttcgcagc ctttgccatt cctttttcac gtaaaagatc aactgctttt tccatgtcgc
     2221 cttctacttc aactaatgct tttttcgcat ccatcattcc tacgccagtc atttcgcgta
     2281 gttcttttac cattttagct gtaacgtctg ccatgtttaa ctcctcctgt gtgtttttct
     2341 ttttaaaaaa agctgcttca aaggagagtg aggcttgtcg cccagccttt gaaacagctc
     2401 catcattcaa aattattctg ctgaagaatt gtcgccttca acaacatcta cgatttcttc
     2461 gattgatgtt gctgtgtttt cttctgtgaa atcttcttca actacttgat cttcaccttg
     2521 gtttccttcg atgaaagcat cagccatttt tgaagtaatc aatttaacgg cacgaattgc
     2581 atcgtcgttt gaagggatta ctacgtcgat ttcatctgga tcgcagtttg tatcaaccat
     2641 agctacgatt gggatattta atttgtgtgc ttcttgaaca gcaatacgtt ctttacgtgg
     2701 gtcaacgatg tacattacat ctgggattct tggcatatca gcgataccgc ctaagaattt
     2761 ttcaagacgt tcacgttctt tgttcaaacc agcaacttct tttttaggta gaacttcaaa
     2821 agttccatct tcttccattg cattgatttg tttcaaacgg ctgatacgtt tttggatagt
     2881 atcccagttt gtcaatgttc cgcctaacca acgatggtta acgaagtatt gtcctgcacg
     2941 agttgcttct tctttgattg cttcttgtgc ttgttttttt gtacctacga ataatgctac
     3001 gccgccttct tcagcaacgc tcttcatgta atcataagct gcatctacta atttaactgt
     3061 tttttgcaag tcaatgatgt agattccgtt tctttctgtg aagatatatt tcttcatttt
     3121 tggattccag cgacgagttt ggtgaccaaa gtgtacgccg gcttctagta attgtttcat
     3181 agaaattact gccatgtttg tttcctccga tttggtttta tttttcctct tcttatcttc
     3241 taccttgccg gcaaactttt tcaagcacct accgacaatc caataagaat gtggatttgc
     3301 tgatgcataa agcaccctca gctattatac agacaaaaga tccgtatcgc aagagcaaat
     3361 atacttttct gctcaacatt caatagttta cctgaaagat aaaaagaatt caagaaaaac
     3421 agtagatttc tctttgtttc tttattatcc tttttccata aaataatttt aggtataaaa
     3481 gacgaatatt attgggcaat ccttcttttt tttgttatga tacatgtgaa atctttcgta
     3541 aaggagaaag aaaatgcgca aagctcaaag acatttgctg attaaacaag tcattgaaga
     3601 attcccgatc cgtacgcaag aagaactatt aaacaaactt gggcattatg gcgttaccgc
     3661 cacgcaggca acgatttctc gagatatccg tgatctaaaa atcgtgaagg cacctgatga
     3721 aaatggcgtt tcccgcttcg tattgtttcg cgaaaaagga actcctgaga ctaggactga
     3781 ggaagaggat cggctggttc aaatgattga agacattgta ttgaaagtcg aacgagtcca
     3841 ctttttgacg atcgtcaata ccttgccaga caatgctcat ttatttgcat ctgttttaga
     3901 tgagatcaaa cctccctcta ttgtcagtac aattgctgga ttcgatacga tcatggtcat
     3961 ctcggaaaat gaggaggacg caaaacgagt agaagaattt cttcaccatc caactgaagt
     4021 atcacttttt taatcagagc acgtattcct aatatggaat gcgtgttttt ttattttcaa
     4081 tattttcctt attgaataaa cctttacact ctttacaaaa atacatcatt ttttattcaa
     4141 atttttcaac accttgattg aatcacaata atcatcattt atattggatg ttaacaagag
     4201 cagcttcttg ctctttgtgt atttcaaaaa agaaatgaaa ggaaatcata tgtataaatt
     4261 attaacttct ggaccattga cgacttctga aacagtgaaa aatgctatgc tgatcgatca
     4321 ttgtacttgg gatcaagaat acaaagaaat tacacaatgg ataagaaaaa aattattgga
     4381 tcttgctcaa gtatcggaag gaatatacac aacggtctta atgcaaggta gcagctcctt
     4441 cggggtagaa tcggtattaa gttctgtgat caggccagaa gatactgtac tgatttgttc
     4501 aaacggcgca tatagacaac gaatgatcaa catgtgcgaa tgtctgaaac tgaactatac
     4561 cgtttatgag gtatcggaac atcaaatcct ccaagctcat gtgattacac agctgattga
     4621 agagaatccg tctatctccg cactagcaat ggtccatagc gagactactt ccggtatttt
     4681 gaataatttg gaagagattg ctgctgtcac acaagaaaga gatcttttgt ttatagtgga
     4741 cgcgatgtca agctttggtg ggataccgat ttgtgtagcc gaactaggga ttgatttttt
     4801 gatttccagc tcaaacaaat gtattcaagg tgttccaggc ttttcttttg tacttgctaa
     4861 taaagcggaa ctggaaaaac gaaaaaatgt tagccgaagt ttgtcacttg atctatatga
     4921 ccagtaccgc gtgatggaaa acgagaatgg caaatggcgt ttcacttccc ctacccatac
     4981 ggttttggct tttcaacaag cgctgaagga actagaggca gaaggaggaa tcgaaactcg
     5041 atattcacgt tatctcgcca ataatcatgt gctacgacaa aaaatgacag agatggggtt
     5101 ccagcctttc attcaagaat atcaggggcc attcatcacc tcttttcttt accctaagga
     5161 ctggcgattt gattttaatc atttttatca gtatttaaag caaaatggat ttgtgatcta
     5221 tcccgggaaa atcagcgatc atgaatgttt tcgtatcgga aatattggcg agatctatcc
     5281 ggaagatatt ttggaactga cagcaattat caaagactat aaggagaacg ttaatgatta
     5341 aaactgtaat ttttgattgg gcaggaacaa ctgtggactt tggctgtatg gctcctgtcc
     5401 acgcttttag aaatgctttt ctagaaaaag gaatccaact gactgacaaa gaaatccgct
     5461 aaccgatggg aaaattaaaa tgggatcata tccaacagct tctttccctc ccttctgtca
     5521 aaaaacaatg gatagctatt tatggacaac tacctcagga aaaagaggtg gaggaattgt
     5581 atcaacaatt tgaacattat ctgtttaaag atttggtcaa gcattctacc ttgaaacccg
     5641 acacattgga aacgatcgaa cggttgagaa acgctgggat caatattggt tccacgaatg
     5701 gatttaatgc tcctatgatg acaatcgtag cagaaaccgc aaaaaaagaa aggtattcac
     5761 cagacttcat ggcaactttt gaagacgtcg aagggtatgg tcgaccgttt ccttatatga
     5821 tccataaaaa catgcagtac ttcaaaaaca agtcggttga tgaagtaatc aaagtcggag
     5881 atacggtctc agatatccag gaagggaaaa atgccggagt gttgacagta ggtgtaattg
     5941 aaggtagttc gctaatgggg atttcttatg atgagtacca aaaattagag atggggaaaa
     6001 agatcgttta aaacagtctg ttgctcttag attccaacaa gccggcgctg atatttgtgt
     6061 aaacaattta aaagagttaa cagaatacat tttgtacgct gacaagaatc cagaatgact
     6121 tttatacaaa ataagaggtt gtgaaaaagc tgtcctgtat caagtaataa aaacagccaa
     6181 gcaaaaatag ctcctcatat ttttcgtttg gttaggctta ttaccgcaga tgcaagcttt
     6241 tgaacaccgt ttattcgaaa aaaaaaaggg agctgtgaca cgacttttgt cacagtcccc
     6301 ttatttgcaa acaaacagtg tttaagagac aacctctcca ctaaacggtg ttcattcagc
     6361 tgactctcgg tatttgctca aaaaacgata aaatttgaaa agctattttc tcttttgttt
     6421 ctctaatact cggatcagaa cgctgatttc acattctctt tttcttacat cttatctggt
     6481 gcatgaagtc ctagcaaacg aaggtcttct ttcaataata cagtcactgc ataaactaga
     6541 gctaagcgtg cttctttttg ttcatcatca gctaaaatct tcgtatgagc atagtatttg
     6601 ttgaatgctt gtgctaattt gattgcatgt ttcgcaatca ctgacggttc gtatttttcc
     6661 ccagcagaca agaccgtttc agggtatttt tgaactaatt taaccacttc ccagctggta
     6721 tcgtcattca atgcatagtc tgctgtttct gaagggacaa atcctgcttt ttccaacagg
     6781 ctgactgcac gagcatgtgt atattgcaca taaggtccag tttctccttc aaatcgaaca
     6841 acttcttcca ggttaaagtc aaatgtattc agtcgatcgt ttttcaaatc gtggaaaatg
     6901 actgccccaa ccccaacttg tttagctaca gcatctttgt tttccaaatc agggtttttc
     6961 tcgctgattt gttctttagc agattggatt gcttcattta atacttcttc taataagacg
     7021 atctttcctt tacgagttga taatttttta ccaccttgcg tgatcaaacc aaatggaata
     7081 tggtgcatgt cgtcagacca gtcaaagccc atttccttca agacagcttt caactgttta
     7141 aaatgatagc tttgttcatt ccctactaca tatagagatt gtttgaagtc gtaagtacgt
     7201 ttacggtaca atgcagcagc taagtcacgt gtaatgtata gtgtcgcacc gtctgacttc
     7261 ttaatcaacg ctggattcag gtcataagca gataggtcaa cgatctctgc acctttatcc
     7321 tcgttcagca aatgtttttc ttctaaaagt ttgacgattt catccatctt gtcattgtag
     7381 aaagcttcac cgttcaacga atcgaaacgt acttccagca gatcatagat tttattgaat
     7441 tctttcatcg attcatcacg gaaccattgc cataattgaa tagcctcttt atcgccttct
     7501 tccagacgtt tgaaccaagc acgagcttct tcatttaatt ctggttctgt ttcagctact
     7561 tcatggaatt ggacgtataa acgaagcaat tcattgattg gttctgcttt aacagcttct
     7621 tctgtacccc attttttgta agcgacgatc agttttccaa actgtgttcc ccagtcacct
     7681 aaatggttga tgcggattgg ctgataaccg attttttcca taatgaaacc gatcgaatta
     7741 ccgataactg tcgaacgcaa atgtcccatt gagattggtt tggcaatatt aggagaagac
     7801 atgtcgattg gtactgttcc ttgattcccg atgttgctat ccccataatg ttctttttct
     7861 ttcactacgg tttgcaaaac ttttttgctg ataagttctt tattcatgaa aaagttcaag
     7921 taaggtccga caacttcgat tttttcaaag ttggcagaat cgattttttc tgctaaatct
     7981 gcagcgattt gctgtggcgc ttttcggtaa acttttgcta atgaaaaggc tggaaatgca
     8041 acatctccgt gttcagctga ttttggattt tctagtaatt gctcgacttg ttccagtgtt
     8101 aaatcatctt tgacagcgtc atagacagct tttgcgacaa gatctttatt attcatcttt
     8161 attcctccta aagtgttccc ttgattgtta aacaaaaaaa atctctagcg acggcatact
     8221 tagtatgcca tcactagaga cgagatttcc cgcggtacca ctccaattgt cctaaaaaag
     8281 gaccagctcc atctgatatc ggtagacgac cgtctgttta aaacagaatt ctccaagtgc
     8341 gcttcgttgt atcttcttca ttcggcttcc accacttccg aattcgctag ccaagaatga
     8401 tacaagtact ctcttgttca acaaatctta gtcttattaa actttcttga attatatcag
     8461 aaaaaaaaac gaaaggctag tcgtttcata aaaaaaaaat ccagatggcc taaaggagaa
     8521 gaaataccac ctggatagct ataaatcatt tcgtaaatac gaaagactta acaaattaat
     8581 cagctacgat aatcgttcct tcttcagaag ctaaaagatt ctcgatattt tctaaagaag
     8641 tgatgattgc cttagcgttt ggttttgctt caacaaatgc aatagctgct tctactttcg
     8701 gaagcatgct tcctggagcg aattgctttt catcaatgta ttgtttcatt tctgaaacag
     8761 taacagtttc taatttcttt tgatctggtt tttggtaatt gacatacaca ttgtctactc
     8821 ctgtcaatac gatcagcaaa tccgcatcga tgatttcggc taatttttct gaagcaaaat
     8881 ctttatcgat tacggcttct cgtccttcta atccagtagc tgtttctaca acaggaatcc
     8941 caccgccacc aacagagact gtgataactc cttgatcgac cagcgtttcg atgacacgtg
     9001 cttctttaat cgagatgggt ttaggggaag cgactacttt tctccatcca cgtccagcat
     9061 cttctttgaa tgtcacagtg gagtcagcat tcatttgttc tttggcttct tcttccgtgt
     9121 agaatggacc gactggttta cttggatttt tgaatgaagg atcattttca tccacaataa
     9181 cttgagtgac taaagaaacc acatctttat cgatcccttt ttccttcagt acttcgccca
     9241 tggcgttttg taaccagtaa ccgatagatc cttctgtcat tgcaacacat gtatctaaag
     9301 gcattgccgg tgttttctca gaatcagcag cttgctgctg aatcaatagg ttaccgactt
     9361 gcggaccatt tccgtgggag atgatcaatt catctccttg ttcgataaat ttcactaaat
     9421 actttgctgt ttccattaac gcttcttgtt gggcttttgc acttgcatcc gtagacaaga
     9481 tggcattgcc ccctaaagcg acaactactt ttcgtttggc catttcattc gtcctttctt
     9541 cagttctacc gaattttcta gataacaaaa cgagccagcg ttttgtgatt ttccctcaat
     9601 gctgactcgt tttttcgtga gatgaatgtg gttatgctta aacgcgaggg ataaacaagt
     9661 tacctaatgt tgctgccatg atcgctttga ttgagtgcat acgattttct gcttggtcaa
     9721 attgacgagc atatttgctg cggaagactt catctgttac ttccatttca gtgatgccaa
     9781 agcgttcttt catttgttca ccgtaaacag tgtttgtatc atggaaagct ggcaagcagt
     9841 gcaagaagat caaacgatct gtgttgtgtg ttttttctac catttccata ttgatttggt
     9901 aaggtttcaa caatttgata cgttcttcga atttatcttc ttcacccatt gatacccata
     9961 catcagaata aaggacgtct gctccgtcta cacctttatc aacatcgtcg gtaatcatca
    10021 attgtgcacc ggatttttca gcaaagcctt ctgccatttt gacgatttct tcttcaggtt
    10081 gtaattcttt tggtgcaacg atacgcatat tcgctcctaa aattgcacct gtgactaata
    10141 aagagttagc catattgttt cgaccatctc cgcagtaagc aacagtgata ccttctactg
    10201 ttccaaagtt ttcttggata gttaaaaagt cagcgatcat ttgtgttgga tgccattcat
    10261 cagtcaagcc attccaaact ggaacaccag agaattctgc tagttcctct accatttttt
    10321 gactgaatcc acggaattcg attccgtcaa acatacgtcc tagtacttta gctgtatctt
    10381 ctgtggattc ttttttacct aattggatat catttgcacc tagatattca ggatgtgcac
    10441 ctagatcgat cgctgcagta gtaaatgcag aacgagtacg tgtagatgtt ttttcaaata
    10501 aaagcgcaat attttttcct tctagatagt gatgtgggat accacgtttt ttcaagtctt
    10561 tcaagtgttc tgaaaaatcg atcaaatatt gtaattcttc ttttgtaaag tctttttctg
    10621 ctaataagct tcttccttgg aatactgatt ctttcatatt aaacattctc ctttagattg
    10681 tttttattta agattaaaga ggaaatcccg acggcagctg acagtaagac tgccgtcagg
    10741 aaacaaatta cttcaagtct tcacggacaa gtggttggct catgcagcgt ggaccaccac
    10801 gacctcgtga caattcactt gaattgattt ctaacacttt gatgccatag ctacgaagca
    10861 attcgttcga tacataattt cggttataag tgactacaac acctggagcg attgccaatg
    10921 tgtttgatcc atcattccat tgttctcgag gtgcaacgat ctcatctcca ttaccagttg
    10981 ggatcagtac aagatcatct aatccaagag cagctttcat cgtttcgtgt aagttatctg
    11041 aatgagtaat cttgatatca tcaccatctg gttcgattgt aaagacatcc atcttaccgt
    11101 ctttactttg aattgctgga tggatagtga atttatcgtg atctaccatt gtaaatacgg
    11161 tatccaaatg catcattgca cggttgttcg gaattttgat tgccaacact ttttcaaatc
    11221 ctgaattttt tgcgaatagg tggcgtgcta atttttctaa agctttcgca ttagttcgtt
    11281 gagaaatccc aacagctact actttatcgc ttaagatcaa ttcgtctccg ccttcgatat
    11341 gatctggatt ttcacgatcc aaccatactt cgattccttt atttgcaaat cttggatgat
    11401 gtttcaaaat atattctgta aacatcgatt cacgacgacg tgcttcaaaa gtcatcttat
    11461 tgactgtcat cccatttccc attgatgcgg aagggtcgcg tgtaaagtac aagtttggca
    11521 ttgggtccat gtaaaatgga tagtcatcgt ctgctgacag atcatataga ctgctagaac
    11581 gtacttcgat atcttttgta cgaaccccag ccatgatttt atctaccatt tcttgcgttt
    11641 ccattgatag aaggaactca tgaagaccct ctcttacagc attcgatgca atatgagatt
    11701 catccaacat tttattcaag aattgttctt tgacattgcc tgcgtcaatc gcttctgccg
    11761 ctaatttttc caagtaaaga gtctcgacgc cttcattttg caatgtcttc gcaaagttgt
    11821 catgttcttc ttgagcaatc ggaagatatg gaatatcatc gaacaacaga cgatccataa
    11881 tatctggtgt caaattttcc acttcttgtc caggccgttt cagtaaaact gtctttaatt
    11941 tccctatttc agagaaaacg tgaataggtt tatccatgtt gtgttgcctc cttctttttg
    12001 attacatgta taggatatcc tccttttgta atcactttca taattttgtc tcattcaaaa
    12061 tgagacattc ctcacattaa tattcgataa ttaaataatt aaacgttttt ctatttttaa
    12121 gcgtaattta ttc
//
LOCUS       DAHHZJ010000081        12022 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.082, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000081 DAHHZJ010000000
VERSION     DAHHZJ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.082"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            183..560
                     /locus_tag="NBL02_001726"
     CDS             183..560
                     /locus_tag="NBL02_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572409.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            complement(740..2035)
                     /gene="purB"
                     /locus_tag="NBL02_001727"
     CDS             complement(740..2035)
                     /gene="purB"
                     /locus_tag="NBL02_001727"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCD2572410.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(2048..3172)
                     /gene="purK"
                     /locus_tag="NBL02_001728"
     CDS             complement(2048..3172)
                     /gene="purK"
                     /locus_tag="NBL02_001728"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCD2572411.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(3165..3653)
                     /gene="purE"
                     /locus_tag="NBL02_001729"
     CDS             complement(3165..3653)
                     /gene="purE"
                     /locus_tag="NBL02_001729"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCD2572412.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(4069..5376)
                     /locus_tag="NBL02_001730"
     CDS             complement(4069..5376)
                     /locus_tag="NBL02_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HCD2572413.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(5379..5960)
                     /locus_tag="NBL02_001731"
     CDS             complement(5379..5960)
                     /locus_tag="NBL02_001731"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCD2572414.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(6076..6173)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(6532..7824)
                     /locus_tag="NBL02_001732"
     CDS             complement(6532..7824)
                     /locus_tag="NBL02_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCD2572415.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7821..8444)
                     /locus_tag="NBL02_001733"
     CDS             complement(7821..8444)
                     /locus_tag="NBL02_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572416.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(8447..9283)
                     /locus_tag="NBL02_001734"
     CDS             complement(8447..9283)
                     /locus_tag="NBL02_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCD2572417.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(9300..10187)
                     /locus_tag="NBL02_001735"
     CDS             complement(9300..10187)
                     /locus_tag="NBL02_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCD2572418.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(10203..11486)
                     /locus_tag="NBL02_001736"
     CDS             complement(10203..11486)
                     /locus_tag="NBL02_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCD2572419.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
BASE COUNT         3652 a         2669 c         2012 g         3689 t
ORIGIN      
        1 gcaatcatct ttttataaat caacaaaaat atgtacattt aaggcattag attcttttaa
       61 tgttcgtgtt ttatcagaaa cgaaaatctt ataccatcaa caagcgttta catttatact
      121 taatgatggt atattagaat taactaaaaa tttggataat gggttcgagg aggcacttgc
      181 cgatggaaga tgcaataaaa caaggtcagc tttttttatc agaattaaca gctgaagaac
      241 gttcctgtct atataatgat ttgctgtcga tcaaagacgt ttatttatgc aataaagata
      301 ctagttttta tacattccga aaaatccgcc gagcaaccaa gcgctgtgct gaatgccgta
      361 aaaggacttt tctttatcag acagaacaca aaggcgaact ttcaatcagt cagattcatg
      421 aacagaagcg tttcaaaaat cattatcgta tccagatctt attcaatgga caagagatgg
      481 aatacgcttt tctcgaaaac ttgctgcaac atctccataa ccagcatttt agttataata
      541 gccaagccat ttacgcttaa ttcctctatt ttttgtatgt aaagaggcac ggtaaagagg
      601 tagccgcttt taaaatcagg ttcctattat tgataagaaa atataataaa ctaaaagaag
      661 cagaaccgct aacggatctg cttctttttt ggttattttt gctattctat tttaattgaa
      721 ttttgcaact cactcttctt tattccaaac ctactcgttt gaatatcacg tcaacgtttt
      781 tcaaatgatg atgataatca aaggcatcgt ctatttcttc tttagataat accgatgtga
      841 tggttggatc ttcttctaac agcgggcgga actgcacttg gttgtcccaa gcgtacgcag
      901 tttttggctg aacaagatca taagcttctt ctcttgtcat tcccttatcg atcaatttca
      961 gcataacgcg ctggctataa atcaaaccaa atgttgcaga catattccgc ttcatgtttt
     1021 caggaaagac cgttagattt ttaacgatat tgccaaaacg atttaacatg taattcaata
     1081 aaatcgtggt atcaggcaag atgattcgtt ctgctgaaga atgagaaata tctctttcat
     1141 gccataatgt gacattttca taggcagtga ccatgtgtcc gcgcaccact ctggctagac
     1201 cagccatatt ttctgaaccg atcggattcc ttttgtgcgg catagcagaa gaaccttttt
     1261 gacctttcgc aaaaaattct tctacttcac gtgtttcgga tttttgcaat ccgcgtatct
     1321 ctgttgcaaa acgttcaata cttgtagcaa taagcgccat cgctgagaaa tattcagcat
     1381 gtagatcccg agggagaact tgagtagaaa tttcttgtgg acggatcccc aattgtttac
     1441 atacatactc ttcaataaat ggcgggatat ttgcgaacgt tcccactgct cctgagattt
     1501 ttcctgcttc tactcctttt gccgcatgtt caaatcgttc gatgttccgt ttcatttctg
     1561 aataccatgt ggcaagtttc aatccaaaag tagttggttc agcatggaca ccgtgtgtcc
     1621 gacccatcat cactgtatat ttgtgttctt ttgctttttc gccaatgatt tccgtaaaac
     1681 gtttcaagtc ttcccgtaaa atatcatttg cttgcttgat gagatagcca taagcagtat
     1741 ctacgacatc cgtacttgtt agaccataat ggacccattt tctttcttct cccaatgttt
     1801 cagaaaccgc tcgtgtaaac gcaacgacat catggcgtgt taatttttcg atttccaaga
     1861 tccgatcgat atcaaatgat gcatgctcac gaattttttt cacatcttct tgcgggattt
     1921 caccaagttc tgcccaagct tcatctgcta aaatctcgac ttcgagccac gcacggtatt
     1981 tgttctcttc cgtccagata ttccccattt cagggcgtgt ataacgttct aacatgtttt
     2041 ttctcctcta atcccaaata ttcgtttgtt tgatttcttc cagcgtttgc tggatgtctt
     2101 ctgtcaagat agtgatatgt cccatcttgc gacctttttt tgcttgtttt tttccataga
     2161 aatggaattg ccagtctggc ttattctcaa tcaagcgata gctttccagc agttgttctc
     2221 ccaaaacatt gaccattact gcattgctta tcaaacgtac tttttctcct aatggccaac
     2281 cacaaatacc tcgaatatgg gcatcgaact gactcataga acaagcttca atagaataat
     2341 gtccagaatt gtgaggacga ggagctaatt cattcacata tagcccgcct gtttttgtta
     2401 aaaacatttc tacccctaaa acacctgaaa ggctcactgc ttctgcaatc acacgggcaa
     2461 tacgctcagc ctcctcgatc acatcctgat caatcgcggc tggagcaatc gtttcatgaa
     2521 gaatattgtt gtggtggatg ttttccacga ccggaaaagt cgtgaaatct ccttgaccat
     2581 ttcctgccac catgatcgaa atctcttttt caaacgggat ccatgcttct aaaacacagg
     2641 ttccttctct taaaaggtcc atggcaggag ctagatccgc ccgacttttc aaaacatact
     2701 gccccttgcc atcatagccg cctcgagtgg ttttcaaaac acaaggatag ccgatcccgt
     2761 caatcgcatc ttggatatcc gtcggactga cgatcgttgc ataaggagca atcacgatgt
     2821 tattcgtttc caaaaaagat ttttctaaca ggcggtcttg cgtaatcgca agcaagtctg
     2881 tcccttgagg aataaatgac atgggtaaaa tcgcatttaa cgcctctaca ctgacgtttt
     2941 cgaattcata ggtaatgaca tctgttcttc tggcaagctc tttcagcgca aaggtgtcat
     3001 catagacact ttcaatatgc caatctgcaa cttgtgcggc tggacaatcg ctcacaggat
     3061 ccagcacacc tactttaaag cccatttctt ttgcactaat cgccatcata cgacctaatt
     3121 gaccgccacc gacgatcccg atcgttgaac caggcaacaa aggtttaacc aagttgatca
     3181 ctactttcta taacagtttt agccaacatt gaacgtcttt cagccaattt agcctccaat
     3241 tctaaatcat acatggaaag catctgtatc gcaagtagtc cagcattaat tgctcccgct
     3301 tttccgattg ctgtagtagc tacaggtact cctccaggca tttggacaat cgaaagaagc
     3361 gaatccaaac catttaatgt tcgtgattgg accggtacac cgataaccgg caaagttgtt
     3421 tttgatgcga ccatacccgg cagatgcgcg gctccccctg ctcctgcaat aatgattttg
     3481 attccttttt ttcgagcgtt ttgtgcatat tcaaacatca aatctggggt tcgatgagca
     3541 gaaacgacct ttttttcata agacacaccg aattcatcca atatttgaca tgcttctttc
     3601 attgtttccc aatctgaagt actccccata atcacagaga tttttgctgt cattgttttt
     3661 cctcccagtt tttctacatt catattttaa caatcacttt ttttgatgtc aaagaaattt
     3721 cgaatgttaa caaagaaaaa cattttattg ttcgtgttta ttaaagataa aaaaaaggaa
     3781 tgtgacaaaa gttaggtcac attccttttt atggataaac ggtgttctcc tgccaactcc
     3841 tcggtcacaa ggaacaatat tcaacaaaca tgagaagctt ttttttgaaa ttgttccttg
     3901 tgactcggag atgaacggca ggctcacgac ctcttccttc aacggtgttc aatcagctga
     3961 ccctcggtat tagctcaaac aaacggcaaa atacgaggaa ctatttttcc gtttgtttct
     4021 ctaacaatca ggtcaaaacg ctgatttcac aacctttctt ttgctctatt acttcttgaa
     4081 ggcttcttcc aatatttctt catctttttt caatccatta aagatcaaat tcaatatgat
     4141 agccgtaatg ctactcatta cgatgccatt tccagtgaac atttgcaatg tttgcggcat
     4201 ttgactgaat aatgtcggcg taacattgaa acccagccca aatccaacag aaacagcaac
     4261 aatcaataga tttttatcat tgctgaaatc aacttccaac agcatacgga ttccttggac
     4321 tgccaccatc ccgaacatga ctaacattcc tccaccaagt accggctcag ggatgatttg
     4381 tgctaacgca ccgattttag ggaataatcc taaaacaatc aagaagaatg cagagaaata
     4441 gatgggtttt cttgttttga ttcccgaaag ttgaaccaat ccgacatttt gagaaaagcc
     4501 agtataaggg aatgtattga aaataccgcc taagataacg gcaagtcctt ctgcacgata
     4561 tccctttttc aattcttctt ctccaacatt tttcccagta atgtcaccaa gtgcaaaata
     4621 tacacctgtt gattctacca tgctgaccat ggaaatgatg atcattagta aaatcgacca
     4681 tacgtcgaaa gttggtttac caaaataaaa gggttgtgga acatggaaca acggcgcatc
     4741 tccaatcgcg ctcagatcaa tcattcccat gaaagcagct aagatgcttc ctccgactaa
     4801 accaatcaat accgcgattg aacgaatgaa cccttttgcc agcatttgca cgcctacaat
     4861 taggagaatc gtcacaaatg caagtagcag atttgttttg tcaccaaaat cagctgctgt
     4921 tgcattacct ccacccattt tctcgattgc tacaggaatc aaagtcaaac caataacggt
     4981 gataaccgtt cctgttacta taggagggaa tagtttttta attttagaaa agacaccaga
     5041 aactaacaca acaaagatcc cagaagcgat aatagatccg tagatagctc ctactccttc
     5101 attggttccg atcaagatca gtggagctac tgcttggatc gcacatccta aaacgacagg
     5161 caagccaatt ccgaaaaatc ggttaactgt caattgcaat aatgtcgcta atccacacat
     5221 aaagatatca attgaaatca aataagtcat ctgttcttga ttaaagccta gtcctgtacc
     5281 gatcaaaaga ggcaccgcta ctgctcctgc atacatcgct agcagatgct gcagccctaa
     5341 aaccgcagct tttccatttt gtgtttcttt ttccacgatt atgcgtcctc ctcaagaaac
     5401 tcaacttgtc cattcgtcaa ggaagcaata cgcgcaagag aaacgactct caatcccatt
     5461 tcttcaagta actgacgtcc atcttggaaa gacttttcga tgacgatccc gattccttct
     5521 acttgcgctc cagcttgctg gcataattcg atcaatcctt ttgccgcttg tccgtttgcc
     5581 aagaaatcgt cgataatcaa tactttgtct tctgaagata aaaatttacg agaaatagaa
     5641 atcgtacttg tcacttgttt cgtaaaagaa taaacagaag ctgtcaataa ttcttcatcc
     5701 atcgtcaaac tttttgcttt tctagcgaag atcattggaa catctaattc tcttgcagca
     5761 aataaagctg gtgcgatccc agaagcttca atggtcacaa ctttcgtgat tccttgttct
     5821 ttaaatactt ctgcaaaccg ttttcccatt tgttccatca atacagggtc tacttggtga
     5881 gtgacgaagc tgtcgacttt taatacgccc tcgcctaata tacgtccgtc tttttgaata
     5941 cgttcaacta attctttcac tttatttgct cctttatcat ttattaatac cgaaataacc
     6001 gcttttgtgc aaaaacctac tatgtttctg ttaaagaaaa acaccctcca ctttatcata
     6061 agaaaagaag ggtgaaatag ccaatctttt acttatagtc cagcatttac ggtactggct
     6121 agaaactgtc atccatatta tgacgatata taagtaggta tgtaaaacta ataatagtgt
     6181 aaattcattt tcttgcaact gttttgcacg tgaaggcagt ttgccatatt tgaataaaaa
     6241 atgttgtttc aaatattgaa aacagtctaa cactactttc tgaaactgac aacgtggttt
     6301 acttattttt ttgtaaaatc taatgatttt taatgcatta ttcctttttg ttcgggttta
     6361 aatagttatt tttattttct cctatctaaa aaagcagccc acataaaaag aaggcatgac
     6421 aaaaagcatg tcatagcccc tttttccgaa taaatagtgt tcaaaagaca gcctatcggc
     6481 aataaggcaa gattttataa actacgggaa gtaatttccg taaatctatt cttattcttt
     6541 taagctgaca agctcgtcaa tcactactgc ttttcctgaa gcaatagaat gatttgctgc
     6601 gattcctgtc aaaatcgaca aaataccatc tacgtgagtt gctgcccgat tccacggatc
     6661 ttcttctttt tcttcaccaa atagatcatc taataaacgc ggatctcctc cgccatgtcc
     6721 gcccgcttct tgttcgacga ttacctcata tggctcaccg aacatcggat agacatggat
     6781 ggactgaaga acggctgctc cttcatcctt tttatctcca cctgcattga tgtaagactt
     6841 ttcgatcact ttcatttcga tccgtccttt gctgccattg aaagaaacat tgaatccttc
     6901 ccacggcata tacgcgttta gcgaatacgt caaaatcgca ccagtttcat atttgaccgt
     6961 tacgcctagt gtatcttcaa tagagatatc atcggaaaat acactgcggt cacgaatata
     7021 tccactgtct ttttctgctt ctaaatacat ttctcgcaat tcctcattgg ctgacaggtc
     7081 tatagcaaat ggatcagctt tagctagttc gcttccatag catcgactgt aaaatatttc
     7141 tactccgcgt ttctccgcat tatgcatccc ataaaagttt aatccaccga atgcaaatac
     7201 cttttgggga cggctgttca accaaaaatt cactagatca aaatgatggg tagatttatg
     7261 aacaagaagc ccgccgctat tgtgcttatt tcgatgccat cttctgaaat aatctgcccc
     7321 gtgttgcgta tttaacagcc attcgaagtg gatagaaaac acatcaccaa tcacatcact
     7381 ttctatcagt tcctttatct tcgtgtgata cggtgcataa cgataattaa aagtgactcg
     7441 caggcttctt cccgtctgtt tttggaccgt tattatttct tgagcttttt cctcatcgat
     7501 agtcagaggt ttttcgcaaa taacatcata tccttttttc attgcacgaa tgatataatc
     7561 atgatgcgtt cgatctactg acgtaataat gacttcagcc ggccgtacgt catccagtag
     7621 ttgctcaaat tgtgatggca tacatgtctt tacttttttc tgtccatact tgttcacaag
     7681 tatccgattt gcataatcca tccttgtttg agatacatca caaaaaccga ctagttcaca
     7741 agtttgatga tacgtagagg caattgcctc ataaaaaaaa cgcgctctcc caccaagtcc
     7801 gacttgtaca tattttttct tcatttaaag tcattctcct tttcttttaa gcaaaatact
     7861 gtgacaaatg gcagccctgg atacagtaat gcagtgagta aaaaatcatg ctgtatacac
     7921 cagaacagtc catacaaaag gcataaatac cacagaaacc ccttgacgtt ttttgacaac
     7981 agccagatat tccttttgat caaataaaca agactatctg ctttatcttc tttgcagata
     8041 tcaattagcg tactaaaaaa actatgggat acaaaaagca acagacacaa tagaaaagga
     8101 atcagatacc aagctccaat ctttcccact aataaatacc aatcaactgc aaaaataatg
     8161 acaaacagct gaaacaacgt aatgatgaaa cacatccgtt tgatttttcc tttcctccaa
     8221 catacctttt tttctgccaa gtaatggaca gatagataat aagaaggata catcatcaaa
     8281 aaataaatca gatagacaac acgagcagac agcactaatc ctaaagtgaa caaacatcct
     8341 agaactaaag cgaaaagagc attactgata agactggtta aaaaaatccg acagacatag
     8401 tagttgagct tgtaaagaaa catctcttct attggctttt tcatattcag cccttcagac
     8461 cgcttgtact gatcccctgg acaagatatt tattgaagaa taagaaaatc agaaaaaccg
     8521 gtagcagcga caacgtagac atcgcaaaca tactcgcgta agtcgtacta gatccagcgt
     8581 ctgcaaataa tttgatggca atcgataccg tgtaagatct cggcttgttt aaataaatca
     8641 atggtcccat gaagtcgtcc catttccaat aaaactggat gatgatcgtc gtgatgatcg
     8701 aaggtttcag caaaggaaaa ataattttgg taaagacact atacttgctg catccgtcaa
     8761 taatcgctga ttcatctaac tctttgggga tgctgaccat gaattgcatg atctggtaaa
     8821 tgaaaaatgc ttgtccaaaa aaatgtggta aaatcagcgg cacatacgtc ccaaccaaat
     8881 tcagccgatt gtaaataata aactgcggaa tcaaaatcac ttgtgcagga atcatcatcg
     8941 ttactaacat gacgacgaac cagaatcttt ttcctttgaa atcgattctc gataatgcgt
     9001 aagcaataca agtcgagctc agaactgtcc ctatcgtcga aactgcagag atgacaaacg
     9061 aattcctaaa gaaagtaaaa aatgaaattc cagcaaaccc cgcccatcct tttgcgtaat
     9121 tttcccattg aaaagtctct ggaatcaaat tcagcgaacc actcataatc tcttgatccg
     9181 ttttaaaaga acctgcgatc atccataaga ccggataaat catgactaga cctaatagca
     9241 aaataaaacc gtattgaaac catttcgtta ctgtattatt catttcttcc tcctctcatt
     9301 cacacaacga cccatttttt tgatgatttg aaaatcagaa aggtgacagc tcccataatc
     9361 aacagcatga tccacgaaat agcactagca taccccatag aattaaaaga aaaagcttgg
     9421 ttatacacat aaagagcata aaagttcgtg gcatcattcg gaccaccttg ggtaatgata
     9481 aatgcctgcg taaaagtcat aaacgccgcg attgtctgca taaccaagtt atacaaaatg
     9541 atcggcgaaa gaagaggtaa cgtgatcttg taaaacgtct gccatttgtt cgctccatcg
     9601 atctctgccg cttcataata cgagcctggg atctctttga tacctgcagc aaagatgatc
     9661 attgccgaac cgaattgcca agcagacatc aaaacaagag ggatcatgac cagtttttga
     9721 tcgctaaacc aggaaagcgc aggtgcgcct aatcgttcca ggaaaatatt cactaacccg
     9781 tttttagaaa acaactcccg ccaaactaga gcgatagaaa tactgccacc gatcaaacta
     9841 ggcacataat acaaagaacg aaagaaagtc gttcctttga tattcctcgt taaaagataa
     9901 gcaatcaata gaccaaagat cagtttcaca ggtacagatg caaatacata gagaaaagtg
     9961 actcggatgg agttcaaaaa acgggcatca ctcagcatct ttatataatt atccaagccg
    10021 atccatgaga ctggatttcc caaacgataa tcggtgaacg aataataaag tgacaagatc
    10081 atcggtatca ctgttatcaa tagaaatcct aggatgaatg gagaagcaaa tacgtaaccg
    10141 accacattgt cttgatccaa gaaccgtttt agtttttctt ttttcatgcc ttcctcctgc
    10201 tgctatccta gaatgtttga cgcaaactca taaagactct ctgctgcttc ttctgctgtt
    10261 acttcttcat aaatgacttt ttcaactaaa agattgtatt ggtcttgaat ttccggattc
    10321 aaagcactat caaagacatt cacttcgccg ttgtccatat cggaaatcat ttctaaataa
    10381 tcaaatgtcg cttgcaaagt tgcatcatct gcacgagcga gctgttctcg aatattcgac
    10441 atgattggta caccacgttc tccttttaaa atttcattgg cttcttcact gttttgaaag
    10501 aaatcaataa acttagcagc ttcttcttta tgggctgagc tttttgccat actcagcatt
    10561 tgcgaagaac gtaaagggat accagattct ccatctgctt ttctgcgagg tggattaatc
    10621 aactgaatct ccatatcttc aggagcccct gacaatattt ctgtcaactg gttacttgat
    10681 agaaacacca ttgccgcttc tctatcccta accgcctgaa aagacagatc cgtcgtactt
    10741 gcagctccac cacctggttc tggatgggcc cctttgtcta ccagattttt acgcagctgg
    10801 aaaaaatctg ttagatagtc tggattttca aatcccaagc tgttagatag atcttcccga
    10861 ttataaaaat tcaatccttt ctcaacttgt ggtatttgaa taatacagcc agctataaaa
    10921 tcttcgaatc ttgaaatagc atagattcct aattcttcag tgatttgata gctgatttgt
    10981 tcaaattcat cccatgtcca atttgaagct ggttcaggga tatttaattg ttcaaatata
    11041 tttttattgt aagcaatcgc aaacgaattc accccgttag acagtccaac taattgcccg
    11101 tcgtaagtcg tcgttgctaa ataggattca tctgcattag aaatatcaat tatcccttgc
    11161 tccacatatt catctaatgg ttcaatctga ttgatgtatt gaggaaattg attgttcagt
    11221 tggaaaatgt cccagatatc atctgatgct gctagtgtac ctaattttgt gtaatatcct
    11281 tcataatcat aaaattcata atcgatcgtc acatggggat ttttttcttc ataaagctcg
    11341 attactttta ctgttgcatc gtgtcggact tgcgaacccc accaagccat gttcagcgtg
    11401 atttttttgt catcttcttg cccacaagcc gtcaagaaga ataacataac acttaaagct
    11461 aagatgattc tcattctttt cttcattctc tgcacctcta cttccattgt tatttttcaa
    11521 tatactgtat ttttatacag aacatattta ttaaacgatc tttttcaagg ggaataaaaa
    11581 ccaagacttg ccttatcgtt ctgcctagga tgaacaactc aatactttca agaaaaatta
    11641 gatagtgctg attcttcttt catcgctaaa aacacttctt gcttcaatga tgggaggata
    11701 attacgcaaa gaaaaaagga tatcagacaa ctgcccaata tccttgacat agctaaataa
    11761 gcatgcttct tcgtgtgcgc tcttccctaa aaaacagaaa aataactagt aattctgtat
    11821 gaaattaggt acaatataca cgtcgatgca caagcattga ctatggcaac ttgattggtc
    11881 agttcccgct gacttttcaa gccccgacaa tttagctgct actaaattgt cgggtgcctt
    11941 ttttagtttt tattcgtttt tcattctata gaaagcgctt caataaatca agacattttt
    12001 ttgttagaat gctaataata ac
//
LOCUS       DAHHZJ010000082        12003 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.083, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000082 DAHHZJ010000000
VERSION     DAHHZJ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.083"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(111..698)
                     /locus_tag="NBL02_001737"
     CDS             complement(111..698)
                     /locus_tag="NBL02_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572420.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            873..2195
                     /locus_tag="NBL02_001738"
     CDS             873..2195
                     /locus_tag="NBL02_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD2572421.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            2350..3126
                     /locus_tag="NBL02_001739"
     CDS             2350..3126
                     /locus_tag="NBL02_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HCD2572422.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            3119..3688
                     /gene="rnmV"
                     /locus_tag="NBL02_001740"
     CDS             3119..3688
                     /gene="rnmV"
                     /locus_tag="NBL02_001740"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HCD2572423.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            3681..4565
                     /gene="rsmA"
                     /locus_tag="NBL02_001741"
     CDS             3681..4565
                     /gene="rsmA"
                     /locus_tag="NBL02_001741"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCD2572424.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            5016..5645
                     /locus_tag="NBL02_001742"
     CDS             5016..5645
                     /locus_tag="NBL02_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572425.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFGLIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(5769..6878)
                     /gene="ugpC"
                     /locus_tag="NBL02_001743"
     CDS             complement(5769..6878)
                     /gene="ugpC"
                     /locus_tag="NBL02_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HCD2572426.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            7087..7509
                     /gene="mgsA"
                     /locus_tag="NBL02_001744"
     CDS             7087..7509
                     /gene="mgsA"
                     /locus_tag="NBL02_001744"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCD2572427.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            7832..8368
                     /locus_tag="NBL02_001745"
     CDS             7832..8368
                     /locus_tag="NBL02_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HCD2572428.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(8408..10171)
                     /locus_tag="NBL02_001746"
     CDS             complement(8408..10171)
                     /locus_tag="NBL02_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2572429.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(10174..11889)
                     /locus_tag="NBL02_001747"
     CDS             complement(10174..11889)
                     /locus_tag="NBL02_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2572430.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         3739 a         2218 c         2452 g         3594 t
ORIGIN      
        1 tttatgttca attagtttgt gctcttatat gtgtgattga aaatatagat aaaaaaacaa
       61 tcatcggctt tgacttcgat gattgttttc tttattttgt aaaaatccta tcacttgatt
      121 ttatggtaac cttttatttt tgtcttcaaa cggcttggta agagagcagt aagcagtgca
      181 aatgggatcg aaaagacata ccagaaaatc gtacgtccga taccataatg ttgatagagc
      241 tttgtcaaca tcacgtgtaa caagatcaac cctaaaagca aggagccagc tataaaccaa
      301 tgattatccc aatgcatgac ttcactaagt gccatcactc caaaaggata gaaataaaaa
      361 tagatcccga ctaaggtcat ccgccagttc tttgtaaatg ttccgatatt caataaaaag
      421 aaaatcgctg aaagcaaaat accgataggc ggaaaaagcc caaccaaaaa ggaatacaac
      481 atcccccaca agatcatcac aggacctttg atcaatgcaa cgattgcgat gaacaaagtc
      541 agcaatatta gtttttgtga taacgttgca acagtcatta atccaaataa aattaatgtg
      601 aaccaatgcc aatgggactg ctgttgttct ttacgggaaa aatcgacccg gttggataaa
      661 taatctgcta atttcggata agaacgctta aaatccataa actgttcctc cttcgtggaa
      721 aaggtaggtt tagtatacga aaaataaaaa agaaatacat ccgtcttaag agggagtttt
      781 tcactgaaag tggagaaaaa agaatggtaa attaaaatat tctcagttat actgaaaatt
      841 ataccaaatt tcaaagaaga aagaggatgg gaatgaaagg caaactaaaa cgagaaatca
      901 atctgtttgg cgcattagct accgttatgg gaaccgtgat cggtgcaggc gtcttcttca
      961 aaactgctgc tgttactgct agtactcagt ctacaagttt gacattgcta gcttggcttt
     1021 tgggtggttt cttaacgatc tgtgctggat tgacagtggc tgaattagct acagcaatcc
     1081 cggaaactgg tggagctgta aaatatattg aagctgcgta tggaaaattg cccagctttt
     1141 tattaggatg ggcgcaaagc ttgatttact ttcctgctaa tattgctgca ttatcgatta
     1201 tctttgcgac acaattgacg aacttattac aattatctac cgattatctt ttgttgattg
     1261 cagtgatcac cgctgtttct gtgacagggt tgaatttgtt agggacaaaa gtcggtgctt
     1321 ctgtacaatc agtgacgttg attgttaaat taataccaat tgctgtgatt gtcatatggg
     1381 gattattgac cccaggacaa ggaactgtcc agttatttcc aatcgaagct ggaaaagatg
     1441 ttacttttgt cgaaggattg agcagtgcgt tgttagcaac gttgtttgct tatgacggct
     1501 ggttaggtgt cggtgcaatg gcaggggaaa tgaaacggcc ggaaaaagat ttgcctaaag
     1561 caattattct tggtttgagt tttgtaacag tcgtttatct attgatcaat tttgtttttt
     1621 tgaaaacttt gccgattgat catcttgccg gtaatttgaa tgcagcttct gaagcatcag
     1681 acgtcatttt tggaggaatc ggtggaaagc tggtcactat tgggatcttg atctcggtat
     1741 acggtgcatt gaacggttat acactaactg ggatccgcgt gccttatgcg atggctttag
     1801 aagacgagct acctttcagt aaacagctga caaagctttc aaagaagttt acggttcctt
     1861 atgttcctgc ggttttccag ttagcagtag cttgtattat gatgagcctt ggttctttcg
     1921 actttttaac agacatgttg atttttgtga tgtggctatt cagtttactg atttttatcg
     1981 gtgtctttat tttaagaaaa aaagcgccgg aattgccacg tccatataaa gtaccacttt
     2041 atccaatcgt tccgattatt gcgattctcg gtgctatttt cattttaggt atgacgatga
     2101 tgacgcaaac gaaattagca ttgatcggta taggtgttac cttgataggt atcccagtct
     2161 attatcaaaa aaagaaaaaa cgctcagaag agtaaaatat ttcaacaaca gacagaaaaa
     2221 gtacgaacta ggtcctctag atcttcgtgc tttttctatt tcaataaaac aagttcttct
     2281 attttgattt ttgctacttc tgcaagtagt gcttatgtgc taaagtaaga atgaaaagga
     2341 gagataaaca tgatatttga ttctcatacc catttaaatg ctgaacagtt caatgaagat
     2401 attccagaaa cgattcagcg agcacaagaa atgggggtaa cgaaaatggc ggtcgtgggc
     2461 ttcgatacgc caacgattga aaagtctttg caattaagcc atgactatcc aaatatatac
     2521 agtattatcg gctggcatcc gacagaggct ggcagttata cgaaagaaat cgaaaataaa
     2581 ttacaagaac aattgacttt accaaaggtt gtagcattag gggaaattgg cttggattat
     2641 cattggatgg aagacccgaa agaagtccaa gataaagtat ttcgcagaca gattgctatc
     2701 gcaaaagaaa tgaacctacc aatcagtatt catactagag aagcacttga agacacgtat
     2761 caaatcctaa aagaagaaga tgttcgagat atcggtggaa tcatgcatag tttcagtggt
     2821 gattatgaat gggcaaaacg atttcttgac ttagggatgc atatttcatt tagcggtgtc
     2881 gtcactttca aaaaagcatt agacgtccaa gaggctgcag caaatgtacc aatcgaacga
     2941 ttacttgtgg aaacagatgc cccttatctg gcaccagttc cttttagagg aaagcgaaat
     3001 gaaccaggct atactcgtta tacagtagaa aagattgctg aacttcggaa tcttccgaca
     3061 gaagaagtgg ctgcccaaac atggacaaat gcgcaccgat tatttggtct aagcgaacat
     3121 ggataagatg aagatccaag agattatagt cgttgaagga aaagacgata caagaaggct
     3181 gcgggaagta gttgatgcag atacgataga aacaataggg tctgcaatca atgaagagat
     3241 cctgatgcaa attgaacatg cccaagagac tcgtggagtg atcgttttta ccgatcctga
     3301 tttttcagga gaaaaaataa gaaaaatcat tatggaagct gttcctcagg caaaacatgc
     3361 ttttttgccg cgcacacaag ctaaaccaaa gaaaaaggga agccttggtg tcgagcatgc
     3421 aagtgatgct gctattttag aagcattgaa aaaggtcgtg acgccagaca tttcccaaac
     3481 ggctgtttct gaaatcacta gacaagacct tttagactat ggtttaatag ctggggcaca
     3541 agcaaaagaa aaacgagaaa aactaggtga cgagttgcgc atcggttaca cgaacggcaa
     3601 acaattggaa aaacgtttga agatgtttcg tattacgccg aaagaactcg cccaagcaat
     3661 gaaaatagtg gaggaacaac atgagtgaat atcgtgaaat tgcgacaccc tctagaacaa
     3721 aagagatttt attgaaacat ggtttttcat ttaaaaaaag tttaggacaa aattttctga
     3781 ctgaaccaaa tattttaagg aaaatcgtag aaactgcgca aatagatgaa catacgaacg
     3841 tgatcgaagt tggaccagga atcggtgcat tgacggaaca attagccaag catgcaaaac
     3901 aagtcgtagc atttgagatc gatgatcgtt tgatccctgt attggctgat acgatgcaac
     3961 cctatgacaa tgtgactatt gtacatcaag atattttaaa gaccgatttg tcaacagctg
     4021 ttagagaaac atttcatgag gagcttccgt taaaagtcgt agcgaattta ccttactata
     4081 ttacgacacc gatcatgatg cattttcttg aatcagattt ggttgtggat gaactagtcg
     4141 tcatgatgca aaaagaagta gctgatcgta tctctgctga accaggaacg aaagcttatg
     4201 gttcattatc gattgctgtt caatattata tggaagccag tttagcattc atcgtaccaa
     4261 aaacagtatt tgtccctcag ccaaatgtag attcagcgat tttgaaactg acaagaagag
     4321 atataccggc agtcgaagtg acagacgaaa aagcattttt ccgattgaca aaagcggcat
     4381 ttcagcaaag aagaaaaacg ctttggaata acttgcagca ttcgtatggc aaagatgacc
     4441 agacaaaagc atggttagca aaaagcttgg aaacagctgg aatcgatcca aaacgtcgtg
     4501 gggagacttt gtcattacaa gaattcgccg cattaagcaa tgcgatgtca gaaaatatgc
     4561 agtaaaaaaa gaggttgtga cagaagttgt gaaaaagctg tcctgcatca agtaataaga
     4621 acagccaaac aaaaatagtt cctcatgttt ttcgtttgtt tgggcttatt accgcagatg
     4681 caagcttttg aacaccgttt aagaaagagg ttgtgggcct gccgtttaac tctaagtcac
     4741 aaggaacaat ttcaaaaaac agcttctcat gtttttgagt tagttggtct tattacttga
     4801 tgcaagcttt tgaccactat tatgccgaac aaggagatgt gacacaaatt ttgtcacatc
     4861 tcctattttt gtttcttgat caaaaaattc ttatacttga tactcacact cattttgtat
     4921 ggatttagaa aagaatatgt gtcttgacag ttattagtaa gtgagttata ctaagtcgac
     4981 atttatgtgt caagacatat taggagagag aaaaaatgag aaaaaattca gttcgaaaac
     5041 taacgatttc tgctttattg attgggatgg gggtcattat cccaatggtc atgcctaaaa
     5101 tcatgattcc accagcttcc tttactttgg cgtctcatgt gccattattt attgcgatgt
     5161 tttttacacc aggagttgca gtggctgttg cgctaggtac gacatttggt ttctttttaa
     5221 caacgccggt tatcatcgca ttgcgagcat tgtcacatct tgtgtttggg ttgattgggg
     5281 cttggtactt gcaaaagcat ccgagtatcg ttttaaaaaa tggacagttt acttttgcta
     5341 attggcgttt ccaagggttc aattttgtga tcggtgtgat tcattcttta gcagaaatgc
     5401 tagtagtcag catcttttac tttattggta atatgcctga aacatattat tctcaaggat
     5461 atttttatac agtattcatc ttgatgggga ttggtgggct tatccacagt ttagttgatt
     5521 ataatattgc ttattttata gcaggaacac tcagcaaata ttttgatatc cccgtattta
     5581 cggcagcaaa aaaagaaaat cggaaagagt cgattcaagg gtacgcgaag aaaacgacat
     5641 attagacatg atctctcaaa aaaaaaactc tcatctagta atggataata aatcctctaa
     5701 gatgagagtt ttttgtccta aaaagagctg caccaacatc ttctggcaca gctctaacag
     5761 ggggcaattt atggaagttt gatgacttgt tctgtttctt tatcaaagaa gtggcctttg
     5821 ctgatgttga aggcaaggtc gactatttcg cctggtttat ggaaatctct tgcatctact
     5881 tttgagatga actcagtaga accagttttt gtgtaaagca tcgtttcagc tccaagtaat
     5941 tcggaaacga caacttctga tttcactacg gcttcaggtg aagcttcaag ggctacttgt
     6001 tcactgtgga tgtcttctgg acgaataccg aagataactt cttttccttc ataacctttt
     6061 tcaactaata ctttgttttt tccttcagga atacgaagct taagcccatg tccatctgtg
     6121 ataacaccat ttttcaatgt cacattaaag aagttcatgg caggagatcc aataaagccg
     6181 gcaacgaaca tattgacggg tgtatcataa acttctttcg gtgtaccgat ttgttggata
     6241 aatccgtctt tcatgataac gattcggtca gccatagtca ttgcttctgt ctgatcgtga
     6301 gtaacataga tggtcgttgt ttcaagacgc tggtgaagtt tggcaatctc agcacgcatg
     6361 gctacacgta atttagcatc taaatttgat aatggctcat ccattaagaa gacttttgca
     6421 tcccgaacga ttgcccgtcc caatgccaca cgttggcgtt gtccaccaga taaagcagct
     6481 ggttttcgtt tcaagtattc tgtcagacct aagatctctg ctgcattgtc cacacgtttt
     6541 ttgatgtctg ctttatcata tttacgtaat ttcaagccaa aagccatatt atcaaaaaca
     6601 gtcatatgag gatacaatgc gtagttttgg aaaaccatgg caatatcacg gtctttaggt
     6661 gccacgtcat tcatgacttt tccgccgata tttagttctc cttcagagat atcttccagt
     6721 cccgcgatca ttcgcaaagt agttgacttt ccacaaccgg aaggaccaac aaaaacgata
     6781 aattcacggt ctttgattga tagattaaaa tcagttacag aataattttc tgcattgtca
     6841 tattttttat atacgttttt cagagccatt tctaccataa tacgttccac actttctttt
     6901 ttagttttat taactattta aagtataaat gaaagcgtta actaacttca atgtcatgtt
     6961 gaacaagaat tttttctttt ttttgtcaat gtgctagttt taagctttaa taaatatcca
     7021 taagaaataa aaactgatac actggagtaa ataagtatgt taacaatatc aaaaggagtg
     7081 tctcttatga aaatcgcttt gatcgctcat gaccgaaaaa aacctatgat ggtgaaatta
     7141 gcaacagcgt ataaaacaat attggaaaaa catgaacttt ttgctaccgg gacgactggg
     7201 cagaggatca cggaagcaac cggcttgccg atccatcggt ttaaatccgg accgttaggc
     7261 ggggatcagc aaattggagc catgatttcg gaggatcagt tagatttggt gatttttctt
     7321 cgagacccgc tggctgcaca gccccatgaa ccagatgtaa cagcacttat ccgtttatct
     7381 gatgtctatg agattccgtt agctacgaat atcggaacag ctgaggtgct actaagagga
     7441 ctggaagccg gcttcctaga ttttcgaaga atcgtccatg aaatagacaa gcatccactt
     7501 tcattttgat ggataagcgt atggaaaaat aacgtttaat gttcgcctat atgtagctat
     7561 aaaagctaaa caaactgata atatgcgata taatacgaac gtattggata atataaacaa
     7621 taatattagt ttttcgtatt tgcagtaaat gtgaaaatat gctatagttt ctgtgggaaa
     7681 agatcccaaa aataatacag agtagaaaat aaagcgttaa gtgctaagag gatgggatgt
     7741 tgcctcttgg acgaaggacg taatgttcgc ggttttattt tcgcattcgc tgcatgatac
     7801 gatgtagcag ctcttaaagg agatgctaaa aatgaagaaa caccggttag acgcacgtat
     7861 gattgctatt atgggcttat tgattgcatt gatggtcact ttgtcccgac tggtagcaat
     7921 cgagacccca tttattaaaa tcagtgtcac atttattcca caagtgatca tgggcattct
     7981 ttttggtcct ttttggtcag ggataggcgc agttttagct gatttagtag gaatggccct
     8041 cttctcgaaa tctgcattct ttattggatt tacattgaat gcatttattg aaggagcaat
     8101 ctacggattc tttttctatc gaaaagagat cacttggaaa aatgccattc tggctacgct
     8161 tagtgtcacg ctgatcatca acttattttt gacaccatta tggcttgcgt tgatgtacca
     8221 tgtgccattg ttcagctggg tcgtttgggc gccccgcctg ttaaaaacag taatatggtt
     8281 accgattcaa tcaattgcca tttattacgt aggacgtagt attccttaca aaaaaatttt
     8341 aagaagttta gcgatccacg ctaaataaaa aacaagcagt cttcccatcc cagaagactg
     8401 cttgttttta ttgtgctgtg aattgtgctt gatagagccg tcggtaataa ccatcttttt
     8461 gaagcagctc atcatgggtg cccatttcga caatagctcc ctgatccatc actaatatct
     8521 ggtccgcatt tttgattgtg gacaggcgat gggcaatcac aaaactggtt tttccttcca
     8581 tcattgctaa gaaggcttgt tggatttttt tctctgtcaa tgtatcgact gagctggtag
     8641 cttcatctaa gatcaacata gggggattac tgatcatcgt tcgagcaatc gtcaacagtt
     8701 ggcgttgtcc atctgatatc tttaaccctt gaccgccaat cttagtatct aatttttccg
     8761 gcaaacgttc tacaaattca tacatataag aggcttttag ggcatcattg atttctgcgt
     8821 cacttgcttc cggattccca aaacgaagat tttctcgcaa tgtactgtca aatagccaag
     8881 tatcttgtaa caccattcca aaacttttac gtaatgtatc acgttggata gagcggatgt
     8941 catggtcatc gactgtaatt gttccttttg ttgtgtcata aaaacgcatc agcaaattaa
     9001 caagtgtgga ttttccggcc cctgtttttc ctactatagc aatggtttga cctggataag
     9061 cagtaaaatt gaaatcttcg atcaaacgct gcttggggtc ataagaaaaa gaaacatgat
     9121 caaaagagac ttttcctttg atatttttgg gttccaaggc ataggcatct ggcaaatcag
     9181 gcttttcttc tggctgatcg atcaaatcaa atgctcgttc tagaccagaa aaagcggttt
     9241 gcagttgtgt cgtgattcca gaaagttcga taaatggttt agaaaactga cttgagtaga
     9301 tcgtaaagct ggaaatcaca ccaacagtga ttgacgaacc actcttcaat gccaataggc
     9361 cgccaactaa accaatcgat aaataagcaa ggtgatctac aaaacgagaa gacgggtttg
     9421 tcaaggaaga agaaaattgt gcccgttgtc ctttttcata tagatcagca tttagcagtt
     9481 caaattgctg ttgactagct tcttcttgct gaaaagcttt cacgattttc tggttaccga
     9541 ttcgttcggt cacaaagccc gagatatccc caataatcct ttgctgtcta tcgaaatttg
     9601 tttgtgaagc acgagcaacg atccaactca caaggaaaat aattggtgta gaacaaagaa
     9661 cgaccaatgt cagaagtgga cttagacgca tcataaaaat aaacgaaaga atgatgattg
     9721 ccactccaga aaataattga ttgaaggccg cagaaattgc aatggaaata ttgtccatat
     9781 catttgtaaa tcgactgacg atatttccgt gggagttttg atcataataa cgaagtggca
     9841 atcgattcag atgagcaaat gcatcttttc ttaattttgc aacggataag taagctaacc
     9901 gattgcttaa cacttggatg agccattgac tgactacgtt gattaaaacg ataccaccga
     9961 atagaataag taaatggaac agtcgattga agcggacctg tccttctcca atcatttgat
    10021 cgatactttg accaatttgt aacgtcatca caactgttgc aatcccattg atgattccta
    10081 gtatcacagc tagccaaatg tcttttgcat aggcagtaag gtaaggaatg aatcggcgga
    10141 gcgtctgcaa ggataatttt tttactttca taactattct tcctcctgtg atgcgacgat
    10201 ttcctgatat tctgctgatt gagcaagtaa ttcttcatgg gtaccctgag cagcaatctt
    10261 accttgatcc atcactaaga tatgatgtgc ttcttgaata gagcggacac gttgggagat
    10321 aataatcact gtactgccaa gataagtctg tagcgcctta cgcaaatcca agtctgtttg
    10381 ataatccaac gcacttaaag agtcatctaa aatcagtaat gctggcttgg caatcaaagc
    10441 tcttgcaatc gtcagtcgct gtttttgtcc gccagagaaa tttttcccat tttccatgac
    10501 ttttgtatct aaaccatttg gaagctgacg gacaaattct tctgcctgag cgattttcag
    10561 agcttcccaa cattcttttt ctgtggcgtc tttcttaccc cattgaaggt tttcacgaat
    10621 agtcccggta aatagtacag aagtttgggg aacaagagca atttgctgac ggagatgatc
    10681 cagctgccaa gaacgaacat ccattccgtc cattataact gtaccggaac tgacatcata
    10741 gaaacgcgga atcaattgtg tcaaagtcgt tttcccgctt ccggttggac caatgattcc
    10801 aaggactgta tttcgcttca gctgaaatgt aatattttgg agagaaagac cagaatcagg
    10861 agtataacgg aagtcaacat ggtcaaaagc gataattgct tcagaagaaa aatcgggagt
    10921 agtactgacc tcagcttcaa gaatcgaatt ttctgtgtct agcacctctt tcacccgatt
    10981 tcctgaagct tcggcacgtg tgaagatcac gacgaggttt gaaacaacga tcagtgccaa
    11041 aagcatttga ttcatataat tgatcaaagc aagaacttgt ccttgttgca agctaccaat
    11101 atttactttg atcccgccaa ctgtcagcaa acagatgatc ccgacattca tgatcaaggt
    11161 tgtagcaggt gataataggg cagagatatt ggaaacacgt aaataattgt tcgctagatc
    11221 atttgtgctt ttatcgaact tctctgtttc tttttttgtc tgcgcaaatg cgcggatgac
    11281 acgaacaccg ctaagattct gactaacgtg ttcattgagc tgatctagtt tttcttgcac
    11341 tttttggtag agaggaacgg tttttttgat gataaaaaat aaaatgattg agaaaatagg
    11401 caataatgct aagaaaaaca gacctacttc ccagtcgatc acaaaagcca tgaccactga
    11461 cccgatactt aaaaacggtg ctctgatcaa taaacgaatg accattgcta aagctagctg
    11521 aagctgattg atgtcgtttg tcattcgggt gatcaaggta tctgttccaa aatgattcaa
    11581 ctcgttgtga gagaaagtat tgattttttt gattagctga ttacgtaact ctgtaccaaa
    11641 tccttgtgaa gctactgaag catagtattg gcaaatgata gcacaaatca gaccaatcac
    11701 agacatagcg accatccata agcccatttc aatgattttt cctcggtcat tcatttttag
    11761 tccattatcg atcaaggaag ccatcattaa aggcaggaca agttcgaaga cagcttcaag
    11821 aaatttgaag aacgggccca aaatgatttg cttacgatag tcttttgcat aacgtaataa
    11881 ctgaatcata tgattcctcc tattatttat ttgtccacaa ttgaaaaaac aagtactacc
    11941 acattgtagc ttaggaaatt tagaatgaaa agagaacaag ccttttcata aatgaaagtt
    12001 tta
//
LOCUS       DAHHZJ010000083        11823 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.084, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000083 DAHHZJ010000000
VERSION     DAHHZJ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.084"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            260..1774
                     /locus_tag="NBL02_001748"
     CDS             260..1774
                     /locus_tag="NBL02_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HCD2572431.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(2177..3160)
                     /locus_tag="NBL02_001749"
     CDS             complement(2177..3160)
                     /locus_tag="NBL02_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HCD2572432.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(3165..3812)
                     /locus_tag="NBL02_001750"
     CDS             complement(3165..3812)
                     /locus_tag="NBL02_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCD2572433.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(3897..4529)
                     /locus_tag="NBL02_001751"
     CDS             complement(3897..4529)
                     /locus_tag="NBL02_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCD2572434.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(4545..5096)
                     /gene="coaC"
                     /locus_tag="NBL02_001752"
     CDS             complement(4545..5096)
                     /gene="coaC"
                     /locus_tag="NBL02_001752"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HCD2572435.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(5093..5830)
                     /locus_tag="NBL02_001753"
     CDS             complement(5093..5830)
                     /locus_tag="NBL02_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HCD2572436.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(5827..6816)
                     /locus_tag="NBL02_001754"
     CDS             complement(5827..6816)
                     /locus_tag="NBL02_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCD2572437.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            7074..7616
                     /locus_tag="NBL02_001755"
     CDS             7074..7616
                     /locus_tag="NBL02_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD2572438.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            7633..8718
                     /locus_tag="NBL02_001756"
     CDS             7633..8718
                     /locus_tag="NBL02_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2572439.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            8730..9536
                     /locus_tag="NBL02_001757"
     CDS             8730..9536
                     /locus_tag="NBL02_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2572440.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            9537..10364
                     /locus_tag="NBL02_001758"
     CDS             9537..10364
                     /locus_tag="NBL02_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2572441.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            10361..11434
                     /locus_tag="NBL02_001759"
     CDS             10361..11434
                     /locus_tag="NBL02_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCD2572442.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
BASE COUNT         3680 a         2130 c         2473 g         3540 t
ORIGIN      
        1 tggttccaga cctgatccac tagcataata acgacgatga tacgtaataa aatatagatt
       61 ccgtttgtga ttctacctgc agtttttgta ctgttcgtaa aaaaggtttt caaatgattc
      121 ataaaataac tccttcatgt atatccttaa ttatactaag aagcgtgtgt ttaccaaagc
      181 tatatgactt aaagaaaaac agagttgata ttgtgaaaaa aatcacttaa tatagtataa
      241 ggagaaaagg gggatacaaa tgaaaaaatt agttgctggt ttattaacag ccgttgcact
      301 tcttgctatg agcgcatgcg gcgcagatca gaaaaataca gcgcagacaa gttctagtac
      361 gaaggaatct gccgaagtat catcaggcgc ttctgaacaa gtatatacag atccaaaaga
      421 aatgaaagaa tcttatgatg tcgtgattat tggagctggt ggtgcaggga tggcagcagc
      481 tatcgaagca aaagatgctg ggttgaatcc agtaattttg gaaaagatgc ctgttgccgg
      541 aggaaacaca tctaaatcat caagcggtat gaatgcttca gaaacaaaat ttcaaaaaga
      601 agaaggaatc aaagactcga atgatgcctt ctatgaagaa acgctaaaag gtggtggagg
      661 aacgaatgat aaagaaatgc ttcgatactt tgtcgatcac tctgctgatg cggtcgattg
      721 gctagatcaa aacggtatca aactggacaa cttaacgatc actggaggga tgagtgaaaa
      781 gaggacacat cgtccgtctg atggctcggc tattggtgga tacttagtgg aggggttatt
      841 gaataacgtc catgagcggg aaatccctat ttttgtcaac gcagatgtga cagacattaa
      901 gaaaaatggg gaaacggtca atgaagtgac tgtacatgtt caaggagaag aaccaaaaga
      961 ggtcaccgga aaagcaatcg tagttaccac aggcggtttt ggtgccagca aagaatttat
     1021 tgaaaaatat cgtcccgatt tagctgatta tattacgaca aatcaagaag gatcaactgg
     1081 agatggtatc accatgattg aaaaattagg tggacaaacc gttgatatgg ataaaataca
     1141 aattcatcca actgtgcagc aggatgaggg ggtcttgatt ggtgaggttg ttcgcggaga
     1201 aggagcaatc ttggtcgatc aagaggggaa aagatttgtc aatgaaatgg atacaaggga
     1261 taaagtatct gcagcaatta cagcattgcc tgaaaaatca gcttatttga tttttgacca
     1321 aggcgtgcgt gatcgtgcca cagctattga attttatgac caaaaaggat atgtcacaac
     1381 tggaaagaca atggaagaat tagcagaaaa aatcgattta ccaaaagaaa cactagcaaa
     1441 aacagttgat ggatggaata cggatgttgc ggagaaaaaa gatgctcaat tcaaccgtgc
     1501 cacagccatg gaacatgact tatcgtctcc gaattattat gcgatcaaaa tcgcaccagg
     1561 aatccattac acgatgggcg gtgtgaaaat caatacgaac actgaagtat tagataaaga
     1621 aaaccagcca attagggggt tatatgctgc tggtgaagtc acaggtggct tacatggaaa
     1681 caaccggatc ggtggaaatt cagttgcgga gatcattatt tttgggcgac aagcgggcat
     1741 acaagcagct cgatttgctt cggaaaatga atgaaataga acgtaaataa aaagaggttg
     1801 tgaaatcagc gttttgagct aataccgagg gtcagctgat tgaacactgt ttagtgaaga
     1861 ggttgtgaaa aagctgccct gcatcaagta ataagaacag ccaagtaaaa acagcttctt
     1921 atgtttttaa cttggttgga cttattaccg cagatgcaag cttttgaaca ccgtttattc
     1981 ggaaaaagag cgtgggacaa aagtaaagaa cacttttgtc tcacgctcta aacacgtata
     2041 aacggcggaa gcagaagaaa ttccttcgga aataagctga aattcacaaa aatttgaaaa
     2101 gcaattttcg tgaatttctt cttatttctt ggagttaaac gcttctgtcc cagcctcttt
     2161 tttgatcagc atgattttat tcagttatct tcaaaatgat ttttccgatg tttttgtttg
     2221 cttccatata ttcatgcgct tgtttgactg cgctgaattg gaatgtacgg tcaatgattg
     2281 gccgaatctt gccattttct agaaaaggcc cagctttttg cataaaagtt tgtgcgagct
     2341 ctgctttata ctgatcactt ctaggtgtca ataatgtacc aagcagttgg acacgtttcc
     2401 caatcaatac accaaggtct atctcaggca cgatggtccc acctagcata ccaatcaaga
     2461 cccaacgtcc atcaacggct atagaagcta aattcttttc ccagtaagaa gcgccgatga
     2521 aatccaaaat aacatcgact ccccgacctt cagtgatttt tcgtacctct tcttgaaagt
     2581 cttgtttttt atagttgatc aattcatctg caccaagttc tttacaaaag gcgagttttt
     2641 cttctgatcc tgcagtaacg atgattttcg cgtgagaaag ctgtctagct aattggatcg
     2701 ctgctgttcc cacaccactt gctcctgcat ggatcaagac agtttcgcct tcttgtaatt
     2761 ttccaagcca gtaaagtgtt tgataagcag tcaaaaaaac ttctgagatt ccagcagcag
     2821 aaacatagtc tagcgtatca gggagaagga ttgcacgatc ggcaggcatc acagcatatt
     2881 cggcataacc acctagattg actaatccgt atacttttgt cccaggagta aaagtggaga
     2941 tatcactttt attctcaact acctctccag ctacttcgac tcccaaaata gggtagggag
     3001 gtttcaaaga gactgattct cttttcataa tatctgttcg attgacagct gctgtatgga
     3061 ctttgatcag taattcgcct tgttttggtt taggaacagg cacttcaatt tgttctaata
     3121 catctacaga accaggatgc ttgattgaaa tggcacgcat ggatctattc ctcctttgcc
     3181 cattcttgga atgctgtatt caactctttg ctaccttcag aaagtactag agtcgtacga
     3241 gaaatatccg tttcttccac tagagcggca agtacagcag caactgtaga gcgagtgatc
     3301 gtttttgatg ccttctcatt aggcttagtt aattgaatgg aagtaacttc ttcatcgtta
     3361 gtcaatgtga ctggttgaag aatgacaaaa tctaagttag ttcgtttcag ccattcatct
     3421 gcataatgtt tagtaatata ataatctgtc atggactcag gccattttgc tggttcatct
     3481 gcataaacag cactgaccat cagatagcga gagacatgcg cattttcagc tgcgatcact
     3541 gttttgattg cgccatccaa gtctacttgc agaagattct ttccttgaga gccggcagca
     3601 aaaatcaata catcgatgcc tttgaatgct tcggtcattt tttccacgga ccaagttaaa
     3661 tcaaaagaga catacgaaac attctctttt tttactaatg tttgggaagc aacatctctc
     3721 acccctgcaa aaatttcatg ggaacttgga gctaaatctt ttatcaggtg tcggccaatt
     3781 tgtccgtttg cacctacaac aaaaattttc atttggatca tcctttcgac gtcaaatctt
     3841 tccattcaat agtaacacca aaagagaaaa aaatcagaga tcggctacga tgactatcag
     3901 ttagtaagag aagggtgctt gaagtatctt aacaagacat tgcatactgc tgcagtcgca
     3961 agacctgcga cgattccttc aggtatacca cttgtaaaaa taaccgctag aatagctgaa
     4021 taaacagcat ttaaatttgt tccgatgact ttggcataat cttgctggaa taaaaagtag
     4081 atcatattca tcactaaaat cgtattggtc aatgaaccgg aaagtccagc taagaataaa
     4141 gaaacggaat ctgctttttg tttcattagt ttgcgcagac ctttataaac gaaataagga
     4201 accaccccaa tcaagatgcg aggaataaac gcgatcagca aggctttcca gcttccatgt
     4261 tctgttccga tgactgggat aaacggagaa aagacaaaag acaaaggtgt ttggataact
     4321 gtgctacgga tcatgctgat cagtccaaat gtaccgccaa gaaaagcacc tagtcttggt
     4381 cctaaaacga tcgaagcaat gattacagga atatgcatgg ttgttgcatt gattggaccg
     4441 atagggataa aacctaaagg cgtaacagct aataaaatca aaatagctaa aaacatcgca
     4501 gttaatgtaa agttttttgt atttttcata agtactcctt ttttctaagc atttctttca
     4561 ttaagagtca gcatgacctg ttcgatgatc gtctgaatat cagctaaggc accttttcca
     4621 aaatctccac aagctaaaag agattctctt ggttcaattt cctgatagcc gacagatctc
     4681 aatgtttcca gattacgctg agtgatcggg ttttgataca tattcgtgtt catggcaggg
     4741 gcaatcaatt ttggtgttcc cactggaagc gccattgcga ctgttgaaag aagatcgtct
     4801 gcaatgccgt ttgctagctt gccgataatg tttgctgtag caggcgcgat gagaaacaag
     4861 tcagcttgtt ttgctagttc gatatgattg attacctgcg gatttgattc ttgcatgacg
     4921 tctgtatgga ctgcgcgctt cgacagagat tgcaaggtta atggcgtgat aaaatgtgtg
     4981 ctgcttttag acataatcac atctacctgg gcacctaatt tgacgaattg acttgtaaga
     5041 tcagcgcttt tgtaagcaga aatacttcca gtaacgccta gtaagatctt tttcattttg
     5101 attcctccag tctaattttc tcagtaatca atgcagcaat ctcagattta ctttgagctt
     5161 cttccactgt tccatcttta gataagagat agccgtggtg tgtctcgcca tggatttccg
     5221 tcaaatcatt tgcgaaaata aaatcagttt tgttatttat cagactattt tttgcgactg
     5281 ccagaagaga ttcctttggt acatctacta atagtttgaa accaatgatt tttgcttttg
     5341 gctgccattc acgtagataa gaaatgattt ttggtgtttt cttcaaaacg agcacaagat
     5401 gctcggtgtt agaagagatc tttgaaacag tggtatctgt ctgttcattt tccgcaaacc
     5461 aattgtccag ttcttcttgc gttgaagaag tgggtaggtt ttttgccagc tgttcttctg
     5521 aaaaactaaa agcaggagta aaatcactga ctgccatact gtggataatc gcatcgtagt
     5581 gttctttttt cgttagttct tctaattgta aaaataagtc cagtgcagat tcgatcgtat
     5641 aaatcgttat attggagtgt cttttcggtt tcagtgcttg tttcgtagtc acgtaatcga
     5701 ttgtggtatc cggattggca gctaaataat cagctaatgc ttgccctaat cgtcctgtcg
     5761 aatgatttgt aatagcacgt acttgatcga ttttttcagt cgttcctcca gctgtgatta
     5821 gaatcttcat ttatggtcac ctacatttac tagtgttctt ccttggtgtg ttccagcaag
     5881 tagtttagat gctgtttcag gcaattgatc cagagtaatc tcttggacga ccagttcatc
     5941 tgcaatctgc aaatccgcca tctgttgcca taaagaaaga cgttgatcta ttggtacatt
     6001 gacagaatca attccaatca actggatgtt tcgcagaata aaaggcaaga cagtcgcgtt
     6061 tagtttgatt cccccagcat tgccacataa agctactgca ccgttataag agatcaatgg
     6121 aagaagactg cttagctgct cgccacctac tgtgtcgatg acatagtcga tttgctgttt
     6181 tcctaaagct tttgttgtct ctggcagaaa ttcatcaggt cgtacgactt gcaaagcacc
     6241 tagacttttt agccatgtga cagactcttc ttttctggaa aaggcaatga ttgactggaa
     6301 tcctagttta tgtaacaggg ctatcgctgt actgcctaca ccgccagaag caccagtaac
     6361 gacaactttt ttatcggagg tcataccttg acggattaaa gcgttcacac acaacatagc
     6421 agtaaaccct gctgtaccaa ggatcatcgc ttgacgcagc gtcatatttt ttggtagagg
     6481 aactaaccag tctcctggta ctttttgata ttggctataa ccgcctggat gggtgacgcc
     6541 aagcccaaaa ccagtagcaa gtacaagatc ttcttttgaa aaacgtggat tgttactttg
     6601 gacgacagtt ccggataaat caatccctgg agtcatcgga tatgttcgta tcacctgacc
     6661 agactcgctc attgccaatg catctttata attgacatca gaataggcaa tttttaccaa
     6721 gacttcattt tcagccaact cagtaaatgg cagatcactg attgctgtat ggaaaacagg
     6781 ttcctttatt acctgaaaag atcgaaaagc gttcatttgt gagacactcc ttttaaaatt
     6841 ttcttttatc ataccataaa gcctactctt ttattcttgt tcttccgtaa atttagacaa
     6901 aaattaggtg aaaaataaag aatcaaacaa ctagaattta ttccaaaaga taaagaaata
     6961 ttcagcaaaa aaaattttac aaatggttgc ctttttcaat aaatttcgta tactaatacg
     7021 agtggtagta aacaggaagt ttactattag aaaacttcga ttcggaggaa attatggaaa
     7081 ttggtgagaa gttaagaaat ttgcgcatcc aaaagaattt gacacaagaa gaactaggcg
     7141 aacgcacaga tttgtcaaaa gggtatattt cccagttgga acgagattta agttcaccat
     7201 caatggaaac attcttttct attcttgaag tcttaggtgt gactccagaa gaattttttc
     7261 atcaagaagc agcgaatctg caagtggttt attcaaaaga agatcatacg gtttatttcg
     7321 atgaagaaaa cgggtatgaa ttggaatggc tcgtggctga ttcgaatgaa aaagaaatgg
     7381 agcctgtctt gctgactttt gaaggtgcag gagaatataa gacgtttgaa ccttcattat
     7441 ctgaaacgtt tatttttgta ttagaagggg aattagagtt aagcttagga gagacggtct
     7501 atgtggcaaa agcaggacag tcgatctatt atcaagcgac aaaacaacat caattaagga
     7561 atcatctcaa aaaacgaaca aaagtattga ttgttgcaac gcaatcatat ttgtaatata
     7621 gggggtaaaa acgtggggaa accaatcatt tcatttgatc aagtagtgaa acgctatgat
     7681 gatgaaacag tattaaaaaa agtaagtttt gagatcgaac aaggaaaatt ttatacactg
     7741 cttggtcctt ccggctgtgg taaaacaacg atcctacgta ttatagctgg attcaccgaa
     7801 gctacagaag gggacatcta ttttgaaggg aaacggttga atgatttgcc agctaataag
     7861 cgacaagtaa atacagtctt ccaagattat gcactttttc ctcatatgaa tgtttttgat
     7921 aacgttgctt ttggattgaa gatcaagaaa atgtccaaat cagatatcga aaagaaggta
     7981 aaagatgctt tgcgtatggt ccagcttcct ggatacgaaa aacgggagat tagtgaaatg
     8041 tctggtggtc aaagacagcg tgtagcgatt gcacgagcaa ttgtaaatga accgaaagta
     8101 ttgctattag atgaaccgtt atcggcgctt gatttgaaac tacgtacaga tatgcagtat
     8161 gaactgcggg atctgcaaca acgattagga atcacgttta tttttgttac tcatgaccaa
     8221 gaagaagcgt tggcaatgag tgatgagatt tttgtcatga ataaagggaa aatcgtccaa
     8281 agtggtacac ccgtagatat ttatgatgag ccaatcaatc atttcgttgc tgattttgtt
     8341 ggggagagca atatcgtgga cggtgtaatg atcgaagata atctagttga gtttgttggt
     8401 aagcaatttg aatgtgtcga tggcgggatg cgtctaaatg aaccagttga agttgtttta
     8461 cgcccagagg atttgacgat cacgacacca gataaaggaa aactagtcgt aacggtcgat
     8521 actcagctgt ttcgtggtgt ccattatgaa atcatctgtt atgatgaaca gcaaaatgaa
     8581 tggatggtcc attcaacgaa aaaagctaaa gaaggaagca aagtaggatt ggcctttgag
     8641 ccagaagata tccatgtcat gcgtttcaac gagtctgaag aagaattcga tgcacgtctt
     8701 gacagctacg aagaataggg gggacaagaa tgaaaacaat gcgcaggatc tattccgtcc
     8761 cttatatttt gtggctcggt ttattcgtga tcgcgcctgt attaatgatc atttatcaat
     8821 ctttttttga tatgaatggg caattcacgc tgaataatta tctatcttat tttacgtcgg
     8881 ggacatcgct ttctatgacg atcaattctg tatggtatgc atttttgatc acgtttttca
     8941 cgctgatcat cagttacccg actgcttatt ttttaacaaa actgaagcac aagcaattat
     9001 ggctgatgct gattattttg cctacatggg tgaatctgct gcttaaagct tacgcattta
     9061 ttgggatttt cagtgtgcac ggcagcgtga atcatttctt tgattttatc gggatcgggc
     9121 cgaaacagat attattcaca gacttcagtt ttctgttcgt ggcaacgtat atcgagattc
     9181 catttatgat tatgccaatt ttcaatgcat tggaagagat gaatccttct ttgatcagtg
     9241 ctagtagaga tttaggcgcg aatagctttg agaccttccg acgagtgatt tttccgttat
     9301 cattaaatgg tgtgaaaagt ggtgtccaag cagtatttat tccttctctt tctttattca
     9361 tgctgactcg tctgattggc ggaaatcggg tgatcacttt aggaacggct atcgaacagc
     9421 atttcttggt tacacaaaac tgggggatgg gttctacaat cggtgtgatt ttaatcattg
     9481 ctatgttcat cgtgatgctc ctgacaggcg agaagaaaaa aggaggtcgc tcataaatga
     9541 aaaaatttaa atggtcgtat ctttatttag ggatcgtctt tttattctta tatgcaccga
     9601 tcttctatct gattttttat tccttcaatg aaggaggaac gatgaacagc tttacaggat
     9661 ttacttggga aaattatgaa gctgtttttg aagatacacg tttgatcatt atcgtattga
     9721 atacgttcat gctggctttc ttatctgcat tgatcgccac gatcatcggg acatttgggg
     9781 ctatgggaat ctattacacg aaaaaacgac gtgcacggac agcgttgttg agcatgaata
     9841 acatcttatt agtctctcct gatgttatta tcggtgctag tttcttgatt ttctttacac
     9901 tactaggatt cggattaggt tttcagagtg tattgctttc ccatattgct ttcagcattc
     9961 caatcgtcgt cttgatggtc ttgccaaaat tgcaggaaat gaacgattca atggtcgatg
    10021 cggctagaga ccttggtgct aataatttcc aagtagtcaa aaatatcatt ttgccttatc
    10081 tttctccagg aatcatagct ggatatttta tggcatttac ctattctctt gatgattttg
    10141 cggttacatt tttcgtcaca ggaaatggct ttacgacgct ctcagtagag atctattctc
    10201 gtgcaagaca aggaatcagt ttagaaatca atgcgctaag tgcgttagtt ttcttgttct
    10261 ccatgatatt ggtcattgga tactacttta ttagtaaaga aaaccgtcca aaacggctga
    10321 agaaaaacag aagagtacgc gagggggtgg cacggctgcg atgaaaaaat tacaatctct
    10381 gtttttaggt attctcatca ttattgcggt gctgctgctt ggtgttcgtc aacttgaaaa
    10441 aacaagtggc atgagtggag caaaggtact taccatctat aactggggag attatatcga
    10501 tcctagtttg atcacgaaat ttgaaaaaga atatggatac aaagtgaatt atgaaacatt
    10561 tgattctaat gaagcgatgt tcacaaaaat ccagcaaggt gggacaaact acgacatcgc
    10621 tataccaagt gaatatatga tccaaaaaat gatcaaagaa aaactagtac ttccattaga
    10681 tcattcgaag atcaaaggat taaataatat tgatccacga tttttagact tggattttga
    10741 tccagataat cagtattcga ttccatattt ttggggaact ttagggatcg tatataacga
    10801 taaattcttt gatgcaaatc aaattaagca ttgggatgat ttgtggaagc cggaattaaa
    10861 agacagtttg atgctgattg acggtgcacg agaagttatg gggctttctt tgaatagttt
    10921 aggctattct ttgaatagta agaatatggc acagctgaca gaagcagcaa ataaattgaa
    10981 tcatttaaca ccaaatgtca aagcaatcgt ggcagatgag atcaaaatgt atatgatcaa
    11041 tgaagaagcc tccgtggcag tgacattttc tggcgaagct gccgatatga tgtgggaaaa
    11101 tgaacattta cattatgtga tcccaccaga aggatccaat ctttggtttg ataatatcgt
    11161 tatgccaaaa accgtgaaaa ataaagaagg tgcctatgat ttcatcaact ttatgctaga
    11221 accggaaaat gcggcacaaa atgcagagta tattggttat tctacaccaa atgaagaagc
    11281 aaaagcgtta ttgccaaaag acatttcgtc ggacgaacaa ttctatccat ctgatgacac
    11341 gatctcccat cttgaagtct acaaagatct aggatcgaaa tacctaggca tctataatga
    11401 cctattctta gaattcaaga tgtataggag ataatggcaa aactcagtaa gaatggggac
    11461 ttatcaacgt tctaaagagc taaaaatcgt cctttttcta tctgagtttt ccaatcttct
    11521 accaaaagcg gatgaaacgc cgcttttttt gtgttatttt attttttcta ccacgttttt
    11581 ctaccagaga aaagtggaca aaaccattgt aaaatagggc ccgagacggg ctagtaaatc
    11641 taagattgaa aaagttacag aaggataaag taattggaaa ctatgcaaat atacttaacc
    11701 cttctgtaat ctattaataa aactaatata tgaatccagt tcaatatttg tagctatgaa
    11761 aaaacataaa aatcatatta gataaaaact aaattggtag ataaattatt ttgtcagata
    11821 tct
//
LOCUS       DAHHZJ010000084        11787 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.085, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000084 DAHHZJ010000000
VERSION     DAHHZJ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.085"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            355..1572
                     /locus_tag="NBL02_001760"
     CDS             355..1572
                     /locus_tag="NBL02_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCD2572443.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETREKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEASPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(2031..2654)
                     /locus_tag="NBL02_001761"
     CDS             complement(2031..2654)
                     /locus_tag="NBL02_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572444.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWEEYKLTANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLVVLLLTHDGDKVARLKNGKVVTAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     ASSQKNESTELSPSDSGSGQTITGNEENQASIITNESSEGQYQTYGQ"
     gene            complement(2810..4207)
                     /locus_tag="NBL02_001762"
     CDS             complement(2810..4207)
                     /locus_tag="NBL02_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCD2572445.1"
                     /translation="MKRYQFPKNFWWGSAASGPQTEGRISGDGKGENIFDYWYKNEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HHYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFQLFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKAYHEMDQSGVI
                     GIILNLTPSYPRDESNPEDVKAARIADAFFNRSFLDPAVKGEFPSELIERIKELDLMP
                     EIHPDDLTIIKENTVDLLGINYYQPRRIKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEKGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDRNGFEA"
     gene            complement(4229..5200)
                     /locus_tag="NBL02_001763"
     CDS             complement(4229..5200)
                     /locus_tag="NBL02_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCD2572446.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMEKISEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISNRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHAIDGLIVFLSEEEWSGFEMLTKMSIPVVALNCPGGPAGIDTIVVNDR
                     ESVAKVIAHHGGQGHKTIGFIAAAQTDYVARERLAGAKEAVQKDPSIHLEIFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            complement(5207..7507)
                     /locus_tag="NBL02_001764"
     CDS             complement(5207..7507)
                     /locus_tag="NBL02_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HCD2572447.1"
                     /translation="MYDYPYQNPKLTIRQRVEDLLSRMNIAEKVGQVNQHLYGWECYE
                     KNGDKIELTEKLKEHVKWGGGLGALYGLFRSDPWSKTDYKNGIPAKESWKVANLVQEY
                     VLQHSRWKIPVLLVEECPHGHQGLDGISYPTNIGRGNTFNVALLEQSSRLMAKELAAK
                     GVHLALVSTLDLAKDPRWGRTEECFGEDPILSARFSEAVVRGFQGEIISEKVSFLDQT
                     VEEINKKKDQIGVVLKHCIAQGEALGGHNSGTVTIGRREFSDIYGPLLKSTRNAVGVM
                     AAYNDIDGIPCHINRALFEQTLRKEIGYQGIVMADGVALDRLSDVFTDKKTAAAYALA
                     AGIDLSLWDETYTKIAEAIDNQVVDEKLLDQAVRRVLSVKFLLGLFEQPFANDPKEYW
                     DHLMEESEHLNLETAKESITLLKNDGILPLDQQVKNIAVIGPNAHDVYHLLGDYSAPQ
                     TEDRLKKKIVQEIKTEFPQSKVSYAQGCEVRNQEDQEEKLLEAVTLAQNSDVIIAVLG
                     GSSARNFDMEFLRNGAVSSKGINMDSGENVDVASLSLGGYQEQLIEQLYQLGKPIITI
                     LVQGRPYDLMKIESFTNAIATAWFPGQEGGKAIAQMISGRNNPSGRLSLSYPRNSRQL
                     PVYYYQRAASKQENYYDLSGSPFYPFGHGLSYTTFEYSQMEVKTDIDKVIISIKVKNT
                     GSVSGKTSTLVYVRLIDGVVIQRTKLLKAFRKDELRSQEERLLVFELTSEDFSYMDID
                     DKRHYAKKAVIMVEQQEKEIKCNWQE"
     gene            complement(7509..8834)
                     /locus_tag="NBL02_001765"
     CDS             complement(7509..8834)
                     /locus_tag="NBL02_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCD2572448.1"
                     /translation="MKVVDRLSEKMLPIVSKMSSQRHLLAIRDSFIVTMPLVMAASVM
                     VLLNALIFSNATVQRILDLSPLAELASIVNNGTMSILAIIVCYNIGWNLATHYIQTGV
                     IDDPSFSPVHAGILSTAVMFILMSLSSTVTLMDGNTAEATGVFLQSLTSSSGLATAMI
                     AALVSTELFIKLAKMKSFKIRMPEGVPPAVAASFNSLVPEIVVILFFSVVVFTLNHTT
                     GLNVPQLIELAIQTPLKAFVLSVPGILFLQFFSDFLWVFGMHGSSILAPIRQAPLLQS
                     IQENMDAFSVGKEIPNIITEPFTNAFGLIGGGGCILPLVIAILWASKRQEQRSIAKFG
                     LTTCLFNITEPIMFGLPVVMNPIYMIPCAIIPSINLVIAYAATSLGIISKTVAAAPWI
                     TPPVIQSFIATGGDIRAAVLTVILIILDVFLFLPFVLAANKAKLAEGGY"
     gene            9065..9490
                     /locus_tag="NBL02_001766"
     CDS             9065..9490
                     /locus_tag="NBL02_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572449.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DITNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGADSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKEATVTFTKIGNYRLKDKDGNIYSL"
     gene            complement(9988..10689)
                     /locus_tag="NBL02_001767"
     CDS             complement(9988..10689)
                     /locus_tag="NBL02_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCD2572450.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDTFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(10705..11145)
                     /locus_tag="NBL02_001768"
     CDS             complement(10705..11145)
                     /locus_tag="NBL02_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HCD2572451.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVLAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFEKLPVETYVGEYL"
     gene            complement(11145..11720)
                     /locus_tag="NBL02_001769"
     CDS             complement(11145..11720)
                     /locus_tag="NBL02_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HCD2572452.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT         3464 a         2519 c         1972 g         3832 t
ORIGIN      
        1 tcggtgtaag cggttaaaat agtaaggaga taaaaatctt gtggtgaccg atttactcta
       61 tagtattcaa aagtagtttc tcggtattcg tacaaaaaaa acatactaag attagagggg
      121 aacatcattg acgaatgatg ttctcctcta ttttctattt ttagattata gtgaagagtg
      181 aaagaaagtt cgaacaaagc cttgaggact acgagtccct atcaaaaacc gaacttcttt
      241 actgtcattt tagaatcagg tttacgtatg ttggggccat gagcccgtgt ataggaaatt
      301 ggaatcgaaa tgaagcagta aagttctttc aacacttaaa aaggaggaaa acaaatgcct
      361 tacattcttg cattagatgt ttcaatggga cacagctaca gtgtcctata caaagacgac
      421 acctgcttat ttgaagacga gatcgaacac actcaaagag gatttcatgc attactcgaa
      481 gagatccagc acttgcctga acgtccattg attgtttttg aagcaacggg tatttattcc
      541 agaccagtcg aaaagttctt tcaagatcat cacttttctt actgtttgtt aaacccgtta
      601 gaagcgaaaa aacagatgga agaaaacacc ttacgcagtt ggaaaacaga taaatcggat
      661 gcccatcggc tcgcacagac tcatcttaaa aatcaacgtc aaccaaaaga agtacaaagc
      721 aatttttact tagagatgcg cgatttagcc cgtttttatc aagaaattga gaagaaaatc
      781 actcgactgc gcatggattt acacaactgc cttcagttga cttttccaga gcttgaacag
      841 ttcttttcaa acaggctgac cccttacgca ttgacattga tccgtctttt tccccatcca
      901 gattttgtat tggcatctac tcggacaaaa ataaaaaata aactgatcaa cgaaacacgg
      961 gaaaaaatct ccgcgaatcg tgccgagcaa aaggcagatc aaatcatcca ctatgcacag
     1021 tgtgcctatc cagctgttga aaaagacagt attcactgtc aaaaaacgat ctattacgcc
     1081 gaacttctcc aagatttact cgaacaaaaa aaagcactgg cgacacaaat gatcaaaaaa
     1141 gccgaagcct ctccttgctt tttgttgtac caaacgttcc ctgggattgg cgcactcaca
     1201 gccgctttat tgctcggtga attaggagat atcacacgat ttaaaaccca taaacaattg
     1261 aatgcctttg tcggaatcga catccgacgg tatcattcag gtaagtatac tggccaagac
     1321 cgcatcaata agcgtggtaa tcccaaagca cggaaaatta ttttcttcac cattcgaaat
     1381 atgattcgcc agcaacgcgc agctcctaat catattgtcg attattacta caaattaaaa
     1441 aagcaaccta tccctaagaa agataaggtt gccacagttg cttgtatgaa caagcttctg
     1501 aaatgtatgc acgctatggt tagggcacat acagagtacg actatgcgta cgcggtctca
     1561 gtggaccatt aactaaacca cttacagtat accacttctt tgcccagatc gaaaattttt
     1621 tacgaacggg gctttattta gtgtgccttg attttgtcgc tttcgtttac gagctatttt
     1681 tcttgcacct tttacttgac taatcgtagg aaagaggctg tgacaaaatt cttgtcgcag
     1741 ctccctattt cttgatgcag gacagctttt tcacaacctc tccactaaac ggtgttcaat
     1801 cagctgacct tcggtattag ctcaaaaaac ggcaaaatat gaggagctat tttcccgttt
     1861 ttttctctaa tactcaggtc aaaccgctga tttcacaacc tctgattcac ggtgttccag
     1921 aagtagcttc ttcggaaata agccgaaatt ttacaaaaac ttgagaagca cttttcgtaa
     1981 atttcttctt atttcttgaa gctgaccact tctgtcacaa cttctgtttt tcattgtcca
     2041 taggtttgat attgtccttc tgatgactca ttcgtaatga ttgaggcttg attttcttca
     2101 tttcccgtta tcgtttgtcc actgccagaa tctgaagggc ttagttctgt cgactcgttt
     2161 ttttgagaag aagcagttgt tttcttgact tgttcgttgg tctttttcca ttccgctagt
     2221 cgatcggcca ccactttttt gtccactttt tcagaataag ctgtaaccac ttttccattt
     2281 ttcaaacgtg ctaccttgtc gccatcatga gtcaaaagca aaacgaccaa atgtttgtct
     2341 ttgaattgat attgtctgag agaaatcgca ctaagtacgt catcgatatt gataaattga
     2401 ttttcttctt ttccttttgg caaatagatg tccaattcac ctttttcgta attggcagtt
     2461 aatttatatt cctcccacca ataagttgag aattcttttt tgaaattatc tactgattct
     2521 ttgcttgcta cgattttttc tgtttctttc tgagcaagat ctgctgtcat ttttcctgaa
     2581 aatgctgcgg ataaaataag cagtaagaaa aaaccgaata accaaaagag aagaaacggt
     2641 ctttttttcc tcatgccttc ctccactcct atttaatttt gactattcta ttatatacaa
     2701 aaaatgaaat agaaagacaa aaaagttgaa aaagatcgta aataattgaa cttctttttc
     2761 ctgaagcggt aagaacggga attttcatac attctctctt ggctattctt taagcttcaa
     2821 atccatttct gtctgataca tctttgaacc aatgacccga tttcttgatc gttctttttc
     2881 catcttgtgc taaatctact gaaataaaac cgtaacgatt cttataggca ttggtccacg
     2941 accagttatc catgcatgtc cacatatgat aacctaaaca attggcacct tcttgaatgg
     3001 cttgatggac atatttcaga tgagattgaa caaattcgat ccgataatca tcgtggatca
     3061 taccatcttc cttgataaat cgtgcttcat tttctacacc catgccgttt tcagaaataa
     3121 aacagtcgat attcccataa tgatctcgta cattgatcaa aatatcatag attccttttt
     3181 catagatctc ccatccgcga taaggattca ttttacgacc aggcatttca taattgtcga
     3241 agtaatcctc aggcatagga ccgtttttag tatcgattgg tgtttctttt gcttttattc
     3301 ggcgtggttg gtaataatta attcccaaaa gatctactgt gttttcttta atgatcgtca
     3361 aatcatctgg atgaatctcc ggcattagat cgagttcttt tattctctca atcaattcac
     3421 tgggaaactc gccttttaca gctggatcaa ggaatgaacg attgaagaac gcatctgcta
     3481 ttcgtgctgc tttaacatct tcgggatttg attcgtctct tggataactc ggtgtcaaat
     3541 ttaagatgat tccgatcaca ccgctttgat ccatttcatg gtaagctttg atggctaatg
     3601 ctgaagccaa cgcttcatga taaccaactt ggattgcctt tttcatatct gattcttccg
     3661 gataatgcca tcgatacaaa tagcctgcct cgactggaac gattggttca ttatgggtaa
     3721 accatttttt tactttcgag ccaaacaatt gaaaacaact ttttgcgtat ttttcatagg
     3781 cttcaaccgt ttctcggttc aaccatccgc ctttttcttg cagatacatc ggcatatcga
     3841 aatgatacag attaacaaag ggttcgatac cgttttcaat caactcatca aaatagtgat
     3901 gatagaaacg aacggcttct tcgttgattg ttccagattc atcaggtatc aaacggctcc
     3961 attggataga ggttcgaaat gaattatgcc cagtctgttt catcaactgt acatcctctt
     4021 gatacttcgt ataaacttgc gaggtttttt caggtcctac ttgatcaaag aacttttgcg
     4081 gttcgttttt gtaccaataa tcaaaaatat tctctccttt tccatcgcct gaaattcttc
     4141 cttctgtttg tggaccactt gctgcagagc cccaccaaaa attttttgga aattgatatc
     4201 ttttcatcaa agcacctctt tttctttttt agttgattcc atgtatgata ttttccatca
     4261 caatcacttt ttcattttct tcctcttctt ttttgattcg tttcaacagc tgttctgctg
     4321 ctcgttcccc gattttatct gttggtggcg tgatcgtgat ttttggaagg atactcagat
     4381 cgtttttatc taaataatca tagtgactga acgcaatatc cttcccgatt ctgacacctt
     4441 cttgattgaa acattcgatg gcaccaatcg acatgttgta attgctgaca aaaatagcag
     4501 ttgccccttt ttctaataat gcttttgccc cgtaatagcc gctttttcta gaatagtctc
     4561 ctgtaaagat ctctaaatgg atagacggat ccttttgtac cgcttctttg gctccagcta
     4621 aacgttctct tgctacgtaa tctgtttgag cggcagcaat aaatcctatt gttttatgtc
     4681 cttgtccgcc atgatgagca atcacttttg ctacactttc tcgatcattc actactattg
     4741 tatcaatacc tgctggccca cccggacaat tcaatgcgac aactgggata ctcatttttg
     4801 tcagcatttc aaatccagac cactcttctt ctgataagaa gacgatcaat ccatcgatcg
     4861 catgcttcat caggtattcg attttttgtt cgattttttc tggctgatcg gcaaacccgc
     4921 ttaataataa gctataccca ttttcttcag ctactctttc gatccctgaa acaacctccg
     4981 aaccaaatcg gttagatatc ccgtttatca acagtccaat agagaaactt cgattattct
     5041 tcaatccttt agcaatgatg ttttctttat agtccaacgc tgaaatcgct tctgatattt
     5101 tttccatatt cttttccttc agctgctgac cgtttaaata gcgggaaact gttcccactg
     5161 aaacaccagc catcttagcc acgtcacgga tcgatgccat acattttcac tcctgccagt
     5221 tacatttgat ttctttttct tgttgttcga ccatgatgac cgctttcttt gcataatgcc
     5281 gcttgtcatc aatatccata taactaaagt cttcagaagt gagttcaaaa acaagcagtc
     5341 tttcctcttg agaccgcaat tcatctttac gaaaagcttt caataatttc gttctttgga
     5401 tgacaacgcc gtcaattaat cggacataga caagagtact tgttttacct gaaacacttc
     5461 ctgtattttt aactttaatt gatattatta ctttgtcaat atccgttttg acttccatct
     5521 gactgtattc aaatgttgta tagcttagac catgtccaaa aggataaaat ggacttccgc
     5581 ttaaatcata ataattctct tgtttagaag cagcacgctg atagtaataa acaggtagtt
     5641 gccgactatt tctaggataa gacaaactca atcgtccgct tgggttattt cttccagaaa
     5701 tcatctgcgc aatggctttt ccaccctcct gtccaggaaa ccatgctgtt gcaatggcat
     5761 tcgtaaatga ctcgattttc atcaaatcgt agggtcttcc ttgcacaagt atcgtgatga
     5821 ttggcttgcc tagttgatac aattgttcaa tcagctgttc ttggtatcca cctaaagaaa
     5881 gagaagcaac atctacattt tctccagaat ccatgttgat ccctttcgat gaaactgccc
     5941 cgttacgaag aaattccata tcaaagttcc gcgcacttga tccacctaga actgcaatga
     6001 tcacatctga gttttgagca agggtcaccg cttctaataa tttttcttcc tgatcctcct
     6061 gatttcttac ttcacatcct tgtgcataag aaacctttga ttggggaaac tctgttttta
     6121 tctcttgtac tatctttttt ttcaaacggt cttctgtttg tggtgcagag tagtctccca
     6181 acaagtggta aacatcgtga gcatttggac ctataacagc aatgtttttt acttgttgat
     6241 ccaacggcaa tataccgtca ttttttagta aagtgatact ttcttttgct gtctctaaat
     6301 ttaagtgctc tgattcctcc atgagatgat cccaatactc ctttggatcg tttgcaaatg
     6361 gctgttcaaa taaacctaat aaaaatttga cagataaaac tcggcgaact gcttgatcta
     6421 gtagcttttc atctacgact tgattatcaa tggcttctgc aatcttggta taggtctcat
     6481 cccacaaact gagatcgatt cctgccgcta atgcatatgc tgcagcagtc tttttatctg
     6541 taaaaacatc ggataaacga tccaaagcca caccgtctgc cataacgatt ccttgataac
     6601 cgatctcttt tcttaatgtt tgctcaaaaa gcgctcgatt gatatgacaa ggtatcccgt
     6661 cgatgtcatt ataagcagcc atgacaccta ctgcatttct tgtactcttc aataatggtc
     6721 cgtaaatatc ggagaactct ctgcgtccaa tcgttactgt gcctgaatta tgaccgccta
     6781 aagcttctcc ttgtgcgata cagtgcttta acacaacacc gatctgatcc ttcttcttat
     6841 tgatttcctc tactgtctga tcaagaaaag aaactttttc tgaaataatc tctccttgga
     6901 accctcgaac gaccgcttca gaaaaacgag ctgagagaat cggatcttcc ccaaagcact
     6961 cttctgtcct tccccaacga ggatcttttg ccagatccaa agtcgacacc agcgctaaat
     7021 gaacaccttt agcagccagt tcttttgcca tcaatcgact cgattgttct aataatgcta
     7081 cattgaaagt atttccacgg ccgatattcg ttggataaga aatcccatcc aagccttgat
     7141 gaccatgtgg acattcttct acaagcaaaa ctggaatttt ccatcgcgaa tgctgtagta
     7201 catattcttg gactaggtta gccaccttcc agctttcttt tgcaggaatc ccattcttat
     7261 aatctgtttt agaccacgga tcagagcgaa acagaccgta taaggcaccc agaccgcctc
     7321 cccatttaac atgttctttt aacttttctg ttaattcgat cttgtctccg tttttttcat
     7381 aacattccca accatacaaa tgctggttta cctgtcctac tttttcagca atattcatcc
     7441 tcgaaagtaa atcttctact ctttggcgga tggttaactt tgggttttga tacgggtaat
     7501 cgtacacctt aatatcctcc ttctgccaat tttgccttat tagcggctaa tacaaatggc
     7561 aggaataaaa atacatctag aatgataagt attactgtta agactgctgc tcgtatatcg
     7621 cctcctgtag caataaaaga ttgtataacc ggtggagtga tccaaggtgc agcagcaact
     7681 gttttactga tgattccaag acttgtggct gcgtaggcaa taaccaagtt aatagatggt
     7741 ataatagcgc atggaatcat atatattgga ttcatgacta ctggtaatcc aaacataatt
     7801 ggttccgtaa tattaaataa acaagtagtc agaccaaatt tagcgattga tcgctgttct
     7861 tgacgtttag aagcccacaa gatagcaata actaacggta aaatacaccc gccacctccg
     7921 atcaatccaa atgcattggt aaagggctcc gtaataatat taggaatttc ttttcctact
     7981 gaaaaagcat ccatgttttc ctgaatagac tgtaacaaag gtgcttgccg tataggagcc
     8041 aaaatactcg aaccatgcat accaaacacc cataagaaat cagagaaaaa ctgtaaaaat
     8101 aagatgcctg ggacggataa aacaaaagct ttcaatggtg tctgaatcgc taattcaatt
     8161 aattgcggaa cattcaaacc agtcgtatgg tttaaagtaa aaacgacaac tgaaaaaaat
     8221 aaaatgacaa ctatttctgg tactaaagaa ttaaatgacg cagctactgc cggtggaact
     8281 ccttctggca tcctgatttt aaaactcttc attttcgcta atttgataaa taattctgta
     8341 ctgacaaggg cagcaatcat cgccgttgct aagccagacg aagaagtcag actttgtaag
     8401 aaaacccctg tagcttctgc tgtatttccg tccatcaaag tgactgtact tgataaagac
     8461 atcaaaataa acattacggc tgtacttaga attcctgcat gaacaggact aaatgatgga
     8521 tcatcaatca cgcctgtttg gatatagtgg gttgccaaat tccagccgat attgtaacac
     8581 acgataatag ctaaaatact catcgtacca ttattcacga ttgaagcaag ttcagccaat
     8641 ggacttagat caagtattct ttggactgtt gcatttgaaa aaatcagagc attcagcaaa
     8701 accatgacag aagcagccat tactaacggc atagtaacga taaaactgtc tcgaatggcc
     8761 agcaagtgtc gctggctact catcttagat acgattggta gcattttttc agatagtctg
     8821 tctacgactt tcattatttc atctcctaga ttctaaaatt gaaccggttc atttttaata
     8881 tagcactttt tttaaagatt gcaacacatt tttttaaaac gctttctaaa ggtggtggat
     8941 tttttatgac ttttgaaaaa ttattctatt taatggtatt ttgtgaattt tttctcttcc
     9001 atccgtttga agttttactt tccaatggta taatttcttt atcctatcaa atgggggaag
     9061 tattttgaag aaattaatcg tactatttat tttgtctgcg ggacttttga cattgactgg
     9121 ctgtgatttg tttggcggct ctgaccaatc ctcggacgaa acaaccgatt cttctgtctt
     9181 aaaagcggaa aaagcagata tcaccaacag tgtatatacg ctaaaagtac gtaaagatat
     9241 ggatttatcc gcgggacagt tgacatttac agataaaaaa atggaatgga cacgcacgta
     9301 tcagacacaa ggagctgatt cttctgaatc atctgaagaa aaaacagtgc tgacagatat
     9361 caagattact accaaagatg acgaatatat tatttcagga aaagaaaata aaaaagaagc
     9421 aacagtcaca tttaccaaaa tcggtaatta ccgtttgaag gataaggatg gcaacatcta
     9481 cagtctatag tgccatctgt tggatcttct tttgtttctc cccttctttt ctgctatagt
     9541 aaagaagcaa gatgactgta aaggagacga aatggatgat cgatagtcct gattattgta
     9601 catgtaataa caacaaaaat atccatacag aatttaatga tcaaggaagt ttgagtatat
     9661 gcgacaattg ccacaaacta gtcaaagatt cttttaaatc gtttgaatct tatcctgtca
     9721 aaaattatgt aaaaagcaaa agaagataag caaaatcaag caaaactcac tatggctgat
     9781 ttttatgaac ttcaaactca gcttcttcct ttgatcaagc ttcaaaaact ggaatcagta
     9841 aaacaaacag tggatcattt cagttcacaa atcaaagagt aacgttttat ttatttcaca
     9901 tctttgaata taaaataacc aaacgataga aagacttatt ctacatcacg tagtaatttt
     9961 tctttcccgt cgtttggttt tttactttta gcctaattct tgacctttcg catgctgatt
    10021 atctgtaaaa tattttccag caggctttcc ttccaattca gctaattttt cttggatctg
    10081 acgaatcacc ttttttgcat cttctggatt ttctacaaaa tcgaatgtat ccccatccac
    10141 ttgtatttta ggacatacat caaaatcctc ataccattga ttatagcggg tgttcagtgt
    10201 ttcgtaatag gtgtataacg tttcatcgct ttctagctgt tcatagtcac gcccgcgttt
    10261 tttgatacgt tctagcatcg tgtccaaaga aactttgata tggaccatca aatcaggacg
    10321 tttttttgga gcaacatcat ctaactcgtt tagcatcgta tctaataagc tatcgtactg
    10381 ctgtacttcg atatccgtta cccgacctaa atcagcattt agatggaaca acagactatc
    10441 ttcgtagatg gagcggtcta gcacattgtc atcgttcact aaagcatttt tcattgctaa
    10501 aaatcgctta ttcaaaaaga agatttgcaa taaaaatgcg tactgctcag gatttgaata
    10561 aaaaagcggc aatacttcgt tatcttcgac attttcataa aatggtttag aatcaatctc
    10621 ttttgcaagc atatccgtca aactgctctt acctgcacca atcgttcctg aaatcaaaat
    10681 cactgacata caacccttcc tcttctataa gtactcgcct acgtaagttt caacaggcaa
    10741 tttttcaaaa tcatattcac gtacagcttg gtctgatttt aaatacgtgt gaagactttg
    10801 tgtaatcatc aagttcgtag gtaccgtctc ttcttgtacg atgatcatcg gttttttcaa
    10861 catagttgcc acacctaatt cgtaagccgt tcctggatcg attgtttgat gctcaaagtc
    10921 aaggatagcc aaaacaaatt ctgcatttgt caactcttcc atatcttttt cgtatacttc
    10981 ctgcgcccat ccagcgctga atagttcata attgctttct tggtgatgac gtggagagta
    11041 gaaacttgtg actgttttgt tttcctccaa cgctttttcg atacgactta ctcgatcgat
    11101 ttgctcctct gaaaaaaatg gtcctgctaa atatatttga ctcattattt tgcctccttc
    11161 aataataact gatttttaat agatactgct cgtaattcat tgaccagttc caatggattg
    11221 ttggaaaaga tcacttgatc caaccatttt ttatccataa aatcctcttt tgcaaaagtt
    11281 tctaataatt gttgcaagcc gctgtaataa ccgccaatat tcaatattcc gattggctta
    11341 tcaggtgtca ggccgatcgc tatccaagaa agaagttgcg aaaattcctc caatgtacct
    11401 agacctccag ggaaaacgag gacagcatct cctttttcaa ataataatac ctttctttca
    11461 tcaattgtct ctgtaggaat aaatgtagta acatctttcc caggcagctc agtttcaaaa
    11521 aggccatttg gatagatacc tgtgactttt ccgttatttt ccagtactgt tttactaatc
    11581 actcccataa tcccggagta tgaaccaccg taaaccagtt cgatttcttc ttgtgccata
    11641 tattttccta acgtttgcgc aataaatttg taactttctt tattgccaaa acgggaaccg
    11701 caaaaaacag taagtttcat aaaaaaacac tccctaactc aaaagtctat cttcattcta
    11761 cacgtgtttt tttaaaataa aagacta
//
LOCUS       DAHHZJ010000085        11732 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.086, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000085 DAHHZJ010000000
VERSION     DAHHZJ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.086"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(284..2068)
                     /locus_tag="NBL02_001770"
     CDS             complement(284..2068)
                     /locus_tag="NBL02_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2572453.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(2068..3819)
                     /locus_tag="NBL02_001771"
     CDS             complement(2068..3819)
                     /locus_tag="NBL02_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD2572454.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            complement(3961..4185)
                     /locus_tag="NBL02_001772"
     CDS             complement(3961..4185)
                     /locus_tag="NBL02_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HCD2572455.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            4436..6022
                     /locus_tag="NBL02_001773"
     CDS             4436..6022
                     /locus_tag="NBL02_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCD2572456.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            complement(6088..7863)
                     /locus_tag="NBL02_001774"
     CDS             complement(6088..7863)
                     /locus_tag="NBL02_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HCD2572457.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(8074..8976)
                     /locus_tag="NBL02_001775"
     CDS             complement(8074..8976)
                     /locus_tag="NBL02_001775"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCD2572458.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            9255..11600
                     /locus_tag="NBL02_001776"
     CDS             9255..11600
                     /locus_tag="NBL02_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCD2572459.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
BASE COUNT         3557 a         2404 c         2186 g         3585 t
ORIGIN      
        1 acccaaaaag ccgtccatgg aaccatggga gactcatcag gtttagcttg catcaggcaa
       61 tcactatcca agttgtatcg ttttctttat attatcgaaa gcaaacgctt ttgtcaacgc
      121 tttcttagtg gttattagcc gcttttgact agctgtcgtc gtctttttta aatcgggtga
      181 ctacgccact aacgctcaca gatgaagaat ctgcgaagct ctgttttatt ttcactctag
      241 cgcttagggc aaaatgctac tatgctactg tcacaacctc tttttacaca ccactgcttt
      301 gcagtttgta catatctgca tacaaaccat tttgcgctag aagtgtttca tgattcccac
      361 gctctactat atgacctttg tctaaaacta aaatcaattc tgcatcccga atcgtggaca
      421 gccgatgggc aatcgctatc gttgtccgtc cttgtcgcat ttttgccaat ccttcttgga
      481 tcaatccttc ggtttcagta tcgatatttg ctgttgcttc atccaatact agtattttag
      541 gatctgtcac gattgttcgc gcaaaagtga tcagctgtcg ttgtccacta gaatagctgg
      601 caccgtgttc gatcactttt gagtgatatc cttttggtaa ttcatcgatg aacttgtttg
      661 cttgaacaaa ctcagctgca tggcgaatct gttcgtctgt gatagctgga ttcatcatcc
      721 ggatatttcc tgcaatatct ccataaaaca agaaggcatc ttgcaagacc aatcccattt
      781 tcttacgtaa ctcttccatt ggatattctc gaatgtcatg atcatcaatc aagatttggc
      841 cttcataaaa ttcgtaaaac cgcatcaata cgttgatgat cgagcttttt ccgcttccgg
      901 tgtgccccac taatgcaacg gtctcacccg gattagcgac aaaagagata ttgttcaata
      961 cattgtgctc tccatcataa gaaaagctta cattgcgaaa ctcgatctta cctgaagtaa
     1021 tcgcttgatc tgctccagga ttttgttgcg gtgcatattc ttctgtatcc atgattttca
     1081 aaatacgact accggcaacc atcccatctg taaaggtgct caaaaagtcc atcatttgag
     1141 acattggatt aaaaaattcc tggacatagg tcgtaaatgc atagatcagt ccaacttcta
     1201 cgggagaatg caacgcatcg atcccaaaca aagtcaacgc caacgcaatc gccaatgcat
     1261 agagaaaatt gatgattgga cttaacagaa tcgaattcgt tttgatcatc gcatagcgcg
     1321 tgtctaaata ctcgttgttc acttgttcaa attctttagc taaacgagct tcttggcgaa
     1381 actgctggat aatacgcatg ccggaaatcg attcattcaa tttcgtgttc agctgactta
     1441 atttttcccg catatttcgg taaatacgag aactgaattt ctggtaatac cagatgacga
     1501 ttcctaaaat cggcaagaac accaaagtcc acaaggcaat ttctccattg atttgaaaca
     1561 tggcaacgaa agaagaaacg actgcaaaga tcccagtcaa taccatcaaa aagacatacc
     1621 aaaattcaaa tagcgtttct gtgtcattcg tcactcgaga aacggtcgat cctgccggtg
     1681 tctggtcaaa ataacgcata cctagtgtat gcagcttttc aaataatctt acccgaacat
     1741 actgataggt cttcaatgaa gccatggaat acaaatacca ttggaaaaac caaatgatac
     1801 ttttgactat gactccaaac aagtacacac cagcaaaaaa caaaataact tgtgtcgtag
     1861 ctgactgttt agccagatga tcatccataa aagtttggat gatccttggc aataggacat
     1921 tgatcacaga cagggcaaac gcaaataaga tagcaacgat gaacgttctt ctaaaaggtt
     1981 tggcgaactt catcagtcgt ttgatgatct tcttctgttc tttaagtgga atcgattttg
     2041 tccattccga ttgataattt tcatccatca ggcctcgcct ccttctatct tcgcttctaa
     2101 ttgctgtttt tcccacattc gttcatacca gccacctaat gcaagcagct cgtggtgggt
     2161 tcctcgttca atgattttcc cttgatcgac aacaatgatt tcttttgcat gcatcacgct
     2221 gcttaaacgg tgagctgcaa tgatggttgt tttctctttt cttatttctt ttaaattatg
     2281 caagatagct tcttctgttt tggcatctac tgcagaaagt gcatcatcta agatcagcaa
     2341 ttcaggattt ttgatcaatg ctcgagcaat cgaaatcctc tgtttctgtc cgccagataa
     2401 agagactcca cgttctccaa ctaaggtatc ataacctttt ggcaattcct tgatttcatt
     2461 gttgataaat gctaaagaag ccgcatgctc tacttcttct tctgaaagtt ttggattaga
     2521 aaaacggata ttatctcgaa tggtcatcga aaacaggaag tgatcctgag gcacataacc
     2581 gattgatcct aataaagcat tcaatgaata ttctttgatc gaacgtccgc caaattggat
     2641 tttcccttga taatcatcgt attcacgcat cagtaatttg atgattgtcg tttttcccgc
     2701 accagttttc ccaacaatac ctaatgtttc tccttcttgg atcgcaaatt tgatttgttc
     2761 taatgctggt gattcttctt tcggatagtt aaaagaggaa atagctacat tcagctcgcc
     2821 ttttgccggg gtctgcactg cacccagttg ttccacgata tgcgtctttt catgcaaaag
     2881 ttcattgacg cgatcataag aagcatttcc tctttccaat acgttgaaca atcgaccaat
     2941 cgcaaacatc ggccaaacaa gcataccgat atagctaata aatgagacca tctgcccgat
     3001 tgagatcgtg ccttccaaga catactttcc acctaaaata attgttgcaa cataagacag
     3061 accgataatc aaggtaatga atggatcaaa taaggagtct aaaaaattga ctcgtttgtt
     3121 tttaataatt gcatcttcaa tcttttcttc aaaatccgcg agatcttctt tttcttgtcc
     3181 aaatgtttta atgactttga tcccagtgat actttcttgt gtcttatcat tgatactaga
     3241 aaaagcttct tgcgaatcac gaaaagcatc gtggagtttt gaccccagca ctcgtgaagt
     3301 aacggctagc aaaggcagag gaatcagcgc aatcagtgtt aagcgccaat caataaacaa
     3361 aatcatcgca atgatcgttg ttccgcccgt aatcactgaa tctgcaaagg tcaagattcc
     3421 agctcctgct acattctgga ttgcattcaa atcattcgtt gcatgagcca tcaaatctcc
     3481 agtccgatat ttttggtaaa agagattgtc catttctgta aaatgtttga atagctgctg
     3541 ccgaagttct ctctccaacc gagctgcact tccccaaata ttcgtccgcc agatataacg
     3601 aaaaagatat tgtaagaaag ctgcaaggat caataataag atccaccagg aaatgatctt
     3661 tattgataaa ttcttatcgg cgatctcatc aatgatgatc ccaatcactt ttggaggaac
     3721 taactgtacc aaggccactg cgaataggga gaaaatccca attaaataat gtttcttttc
     3781 ctgacgaaaa aaccaaccta attttctaaa aatcgacatt ttactcctcc tttgtcttta
     3841 tcaagagcat aggattgtct gtgcttgtct tttttgctga atagtctgaa tagtctaaca
     3901 aattacgaaa taaaaaacgg actgaaaaaa tcagcccgca aaaagtcttt gggtcagaac
     3961 ctattttttc ccttggttct tcatttgctg catcatctgg cgaattttct tctcagatgg
     4021 tttttgcccc attgacatca tcattgatcg caacatgtcc tcattaacgg gaggattttt
     4081 ttctaaatag tctttcatat atttacgcgc taggaaaaag ccacctactg cgccgattag
     4141 caaagcaata atagcaataa gtacgacaat acctgttgat accatataaa tctctccttt
     4201 ctatttccga attgctctca atgatttatt ttactaaagt ttattgaaaa aagaaaggcg
     4261 tattctttag tttcatgcaa aaaatagtgg tttttttaga gaagaacaaa atttctttca
     4321 ttaaccagca agacacacca cgaaaaaaaa ttaggcatac cgaatctttt tctttaattt
     4381 tttctttctg aggtgtagaa tgtaatcgta gataaatagg aaaggtggtt ttttcttgaa
     4441 taatcaatcc aacaaacaat ttcataagca tcgacttatt gaacatacaa atggtttatc
     4501 tttagctgaa gtaaatggaa cagtggatgt accaaaggga aaaagttttt ttaaaacact
     4561 tcttgcttac tctggaccag gtgcattagt agcagttggt tatatggatc caggtaactg
     4621 gtccacttcg atcactggtg gacaaaattt ccaatattta ttgatgtcag ttattttgat
     4681 gtctagtcta attgctatgc ttttacagta tatggcagca aaattaggaa tcgtgaccca
     4741 aatggatttg gcccaagcaa tccgagcacg gacaagtaag acattgggtg tcattttatg
     4801 gatcttgact gaattagcca ttatggcaac agacatcgca gaagtcattg gtgcagcaat
     4861 cgccctctac ttactttttg atattccatt ggtgattgcc gtctttatta ctgtattaga
     4921 tgttttcgtt ctcttactac tgacaaaagt cgggtttcgt aagatcgaag cactggttgt
     4981 ctgcttaatt ttcgtcattt tattcgtttt tgtctatcaa gttggcttgt ctaacccaga
     5041 ttggcacagt ttatttgctg gctttatccc taacagtcat acattctcag actcaccggt
     5101 catcggcggc gaaactccgc tgactggagc tttagggatt attggtgcaa cagtcatgcc
     5161 gcataactta tatcttcatt cagcagtttc acaaactcgg aagatcgatc accaaaacga
     5221 agaagatgta gcacaagctg tacgtttttc tacttgggat tctaatatcc agttgtcaat
     5281 ggcttttcta gtcaatgcac tgctattgat catgggtgtc gctgtcttca aaaccggttc
     5341 tgtccaagat ccttcctttt ttggtctgta tgaagcttta tctgatccat cagctatgag
     5401 caatggtatt ttgattgctg tagcaaaatc tggtatcttg tccactttat ttgctgtagc
     5461 acttcttgct tctggccaga actctactat tactggtacg ttgaccggtc aagtcatcat
     5521 ggaaggattc atccatatgc gtatcccgat ttggctaaga agactggtca cacgattgct
     5581 ttctgtgatt cctgtattga tttgtgtttt gtttacaagt acaaaaggaa cgatcgaaga
     5641 acatgccgct ttgaataact taatgaatga atcccaggtg ttcttggctt ttgcgttgcc
     5701 attttcgatg attcctttac tgatgatgac caacagccaa gcggagatgg gtagccgatt
     5761 taaaaattca ttcatcgtaa aacttcttgg ctggttctct gtgattagtc taacttactt
     5821 gaacttacga ggattaccag gacagatcga aagtttcttc ggcgagcaag ccacttccgc
     5881 tcaactagca ttggctgatc acatcgctta tgtaatcatt gttcttgtcc ttctcctgtt
     5941 ggcatggacg attggtgatt tatataaagg aaacaaaaaa tatgaacagc ttttgcagga
     6001 aacggtagaa gaaatcgaat aaaaactgat ttttccacac aaagaccctt gatagttagg
     6061 ttaaaaatct agctaccaag ggtcttttta tttagaagat aaagcttttt tcagatttaa
     6121 tttactgaaa actagcatgc ttgttactac gatgaattgc gttaatggca cgcctgcacc
     6181 agtgtagaca cttaaaaaat agtccttttt ttctggcgca taagaacaaa accatgagac
     6241 accgaaaaga aaaaccaatt gtgtcagtat taatccggtg accacaatca aagaggaact
     6301 ataataggca cgatcagctt ttgcaaatac ggttcctagt atcataagta atatccctgc
     6361 tattgaagca atcagcacgg gaatagccag aacttggtaa atttttatga tgacattcgc
     6421 gacttttgct gattttgtta attggaaatt aggtgttctc ggttcattac tatcgatcca
     6481 attctccatt agcggaaggt ttaataattc ttgagcctgt ttgacttgtt cttgcgttcc
     6541 gaaactataa tttcctcctt gatggaaatc tttatatcgc catgcataag cgtatcctga
     6601 ttgaaggaaa tcgaatacga ttggcagatc actcatcgtc tttccatctc ctgatcccat
     6661 cagatagaac tcttttttct ttttgatggt tcccttgtga taggcttctt ctaattcttg
     6721 gttgactttt ttccaaaaat cattggtttc ttttgcattg ttttggtaat aaccagattc
     6781 gcttaccaca tctcgcaatg cccaaaagat gatatctccg ggtaattctt ttacttttcc
     6841 accttgagtc cacgcgtgag tcgtatataa ttcgtcaata cgggtagaaa cagtatggaa
     6901 tgtaggagat gcggctaatg ctttatttag tgcagcatgt gagacccata tctcactgtt
     6961 ttctttattc aagttcgttt catcatctat tcggatcagt tgtttcatca aacgtccaaa
     7021 ttctccactg gaacggtcat tgatggttgc aataccgtat gttgtctcat ttttatattc
     7081 gatccactga tagacgatgg aaaagccaac tataggaagt aaaaacaatg aaagtttcag
     7141 ccattcccgt ttcgagagaa gaaagagtcg tttttttaac ttctttactt ctttgaaaga
     7201 aaagtcttgc cctagcaaga tctggacgaa actgataccc aaagctacta cagtaaaagg
     7261 aagaagccag atagaatctt cgcgaatgaa ccaaaagaaa ggaaaagcga cagaaagccc
     7321 taaaaaccat ggcagcaatt ttctgctaga accgtatttg cggtaataaa tagctaataa
     7381 acaagcgata atgatctcaa cagccggaat cacgagggca ttacgatatg ttcgagtgga
     7441 atattcatgt gtaaaactaa tcggagaaaa caaaaaataa aaatagatac ttaaaagaag
     7501 atacttgttc ttcacaatcg gtttaaacgc gagtgcaacc aatatactgg caaaaatact
     7561 gaacatgccg aacaaaactg cataagaaag atgcagttta tgtgcaagta ctaaaaataa
     7621 gctgtaggag attcctttgg aaagtgttgt agtcgtatac tcccctagcc atttaccatc
     7681 caaaatattt tgagcatagt gaatcagcag ctgatcatca taggtcgccg acatatctgt
     7741 tccatagatt gctttactat ataaatacaa ccgaataatt gtaaataata cggctatgcc
     7801 aagaataaag tatgtatgtt tcttttttag ttggtcgatc aaggggttta aaatgttcag
     7861 catcttttcg tcctttcaat cacgttttgc tacttgtttg tgaaactttt cgttttttac
     7921 ttttctatca gtataccctg tttgtctgaa tttaaaaaga gggggcaaaa ggagcatggc
     7981 gaaaacgaat gcaaaaagca ctcctttgtt tcttaaacaa aaaaggacaa ggcagactgc
     8041 ccaatccttt tttgttgctg tcaggtagaa aacttaaagt gtgacttctt ctgctaataa
     8101 ctcagggaac acttcgggat cgaatgcttg cgctttgaac atggaaattt cttttccata
     8161 tggtgcgtaa gctttctttt tatctttacc gacatatggg gtttctaata ttttcgaaat
     8221 gttgtcaaac attggatcat ggacgatttt gttcaatgtg tcaaatccaa ttgttccaaa
     8281 accgatattg gcatgccgat ctttatgaga accttgtgga tttttagaat cattgatatg
     8341 cagtacttta agccgatcta atccgatgac cttatcaaac tcttccatta cacctgaaaa
     8401 atcttctttt atattatatc cggcatcatt cgtatgacat gtatcaaaag taacggataa
     8461 tttttcattc aattttactc catcgatgat ttgcgctaat tcctcaaatg ttcgaccgat
     8521 ttccgtacct ttccctgcca tcgtctccaa agcgatctgt gccgtctgtt cttttgtcaa
     8581 aacttcattc aacccctcaa taattctagc aatcccagct tctgcccctg ctcctacatg
     8641 agcgcctggg tgaagggtga tttgagtagc acctaaagct tccgcacgga caatctcttc
     8701 tctcagaaat tgtacagcaa atggaaacat ttctggtttg atcgtatttc ccaaattaat
     8761 gatataaggc gcatgaacga cgatatttgt tagtccgttt tctgccataa atgcttttcc
     8821 ggcatcaata ttcatctctt cgatagcttt tcttctagta ttttgaggag cgcctgtata
     8881 gatcataaag gtcgaagatc cgtaactggc tgcttcttct gccgcgccta acagcatttt
     8941 ctttccgctc atgctgacat gtgagcctaa taacatggat ggattcctcc ttgaaaggca
     9001 attgtttcga acaattcctg tatattgata agatgatgta caaagaaaat ggtttgtttt
     9061 tctttgccct gttctccata gtaactgaaa ctttccctaa agaaaagctt aagaactctt
     9121 tcataaaaaa gatcatttcc tcacgtttta tctcattcga aaaagaaatt cctgtcaaaa
     9181 acagccttgt ctgttttttt gtcctattat ttgctataat tagcgaaata aaaccattag
     9241 ttgaggtgac ccttttgcca cgcaaaaata ctcgaaaaaa acagaataga agaaaaaaag
     9301 aaaaatggtt tgttcctaag attattttta gagtgtttca atcattgact gtttttatta
     9361 ctgttcttct tttgttgttc gcagctctag gtatcggaat aggtgctggc tactttgcct
     9421 atcttgtcga agacacacaa ttgccgacta aaaaagaact gcagacagaa ctaggaaaca
     9481 ttacggaaac gtcaaagatt gtttatgcag ataatacaga gatatcaaaa atccagacag
     9541 atctgatgcg gacgactatt tcatctgata agatttcgcc tttattgaaa accgcgatta
     9601 tcagtacaga agatgaatac ttcgataaac atcaaggtta tgtccctaaa gctgttttac
     9661 gagcactagt ttcagaagcg actggcatcg gctcgtctgg aggatccact ttgacacagc
     9721 aattagtcaa acaacagatc ctaaccgatg aaacaacatt caaacgtaaa gcaaacgaga
     9781 tcgttctttc tgcacaagta gaaaaacact tttcaaaaaa tgagatcatt actacgtatt
     9841 tgaatgtctc accatttggt cggaataata aggggcaaaa tattgctgga gtccaggaag
     9901 ctgctagagg aatctttggt gtagatgcaa aggatgtgac tctgccacaa gctgcttata
     9961 tcgctggttt gcctcaaagc cctatcacgt atagtccata cacgaataca ggcgcgctaa
    10021 aagaagatct ttctgccggt cttgccagaa aagattttgt tttattcagt atgtaccgtg
    10081 aaggacaaat tacgaaagaa caatacgaag aagcgaaagc ctatgatctt acaaaagact
    10141 ttctcccaca acaaatcgcg gaacagactg acagagaatt cttgtattat accgtgatga
    10201 acgaagcaac aaggattatt gcacaacaat tggcagaaaa agataatgct gatatgtcag
    10261 attcatcggt cagtgaagct tattatcaaa aagcacagca aacgatccaa aacaaaggat
    10321 atacgatcca ttcgacgatc gataaagata tctatgctgc attacaaacc ggcgtagaaa
    10381 attatggcta catgttagat gatggtgctg gtagccaagt ggagactgga aatgtattga
    10441 tggataacag aaccggacgt atctatggct tcgtgggtgg tcggaattac agccagaatc
    10501 aaaataacca cgcctttgat acagaacgtc aagctggttc atccatcaaa ccggttcttg
    10561 tttacggtcc tgcaatcgat atgggacttg tcggatcaga atcccgtgta tcagattatg
    10621 caacgacttg gcaagaagga aaaaatgctg gtgagaaaat cgtcaatgcg accaatgaag
    10681 ggtcgaacac cttccaaacc gtccgtgagt cactggaatg gtccaacaat atcccagctt
    10741 atcacttata tcaagatgtg ttgaattccg gtggatcgaa acaatacgct tacgagcact
    10801 acttggcgaa gatgaactac ccagcaaatg ataattgggg cgttgaatcg gctcctttag
    10861 ggacagtaga tgtaacgacg cttcaacaga caaatggatt ccaggcctta gcaaatggtg
    10921 gagtctacga agaaggctat atcattgatt ccatcacaga taatgcaggt aatgtgattt
    10981 ataaacatga aagtaattct gtccgtattt attccgaagc aacggcatcg atcatgaatg
    11041 acatgatgcg tagtgtgatc aatgaaaaaa tcactacacc attcaaagat gtcatctcaa
    11101 gcttaaacgg aaatctagga aatgcagatt gggtcggaaa aactggttct accaatgaat
    11161 accgagattc atggctgatc gtctcaactc catctatcac tatcagtagt tgggcaggac
    11221 acgatgacaa tactggaatg gacagccaag caagaatacg ttcggctaat tatcttgcca
    11281 atctaatcaa tcaagcttac caagcaaacc ctgagatttt cggcactgat gagaaatttg
    11341 acttatcatc cgatgtgatg aagaaaaaag tatctgggtt cacaggacaa acagctggaa
    11401 aagttactgt caataaaaaa tccatccaaa cgccaggaaa aacagtggag tcactttggg
    11461 caaaaaatgg tccgaagaaa agcgccttta aattcggcgt aggcggtact gatgaaaact
    11521 atacagatta ctggaacacc gcttcagctt atgctagagc gaatccagag accaaagaat
    11581 cagacgataa agatgattga tctaatagat taatcagaat taaagggagc tgcgacaaaa
    11641 atcttgtcac agctcccttt ttccgaataa atggtgttca aaagcttaca tctacgatat
    11701 taagacaaat ttatgtaaaa atgtgaaaaa cc
//
LOCUS       DAHHZJ010000086        11711 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.087, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000086 DAHHZJ010000000
VERSION     DAHHZJ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.087"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            91..915
                     /locus_tag="NBL02_001777"
     CDS             91..915
                     /locus_tag="NBL02_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCD2572460.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            947..2257
                     /locus_tag="NBL02_001778"
     CDS             947..2257
                     /locus_tag="NBL02_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HCD2572461.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            2405..3664
                     /locus_tag="NBL02_001779"
     CDS             2405..3664
                     /locus_tag="NBL02_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCD2572462.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            3674..4546
                     /locus_tag="NBL02_001780"
     CDS             3674..4546
                     /locus_tag="NBL02_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCD2572463.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            4558..5388
                     /locus_tag="NBL02_001781"
     CDS             4558..5388
                     /locus_tag="NBL02_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCD2572464.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            5428..7611
                     /locus_tag="NBL02_001782"
     CDS             5428..7611
                     /locus_tag="NBL02_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCD2572465.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            7672..8892
                     /locus_tag="NBL02_001783"
     CDS             7672..8892
                     /locus_tag="NBL02_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HCD2572466.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            8889..10349
                     /gene="gtfA"
                     /locus_tag="NBL02_001784"
     CDS             8889..10349
                     /gene="gtfA"
                     /locus_tag="NBL02_001784"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HCD2572467.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            10426..11532
                     /locus_tag="NBL02_001785"
                     /pseudo
     CDS             10426..11532
                     /locus_tag="NBL02_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT         3945 a         1820 c         2279 g         3667 t
ORIGIN      
        1 atgttccttt tagtgattat attaattatt actgcaattc aaatgaagtt gcagaaaaag
       61 tgggtaaact attaaggagg ctatcaaatt atgaaaaata aatttttttc aaatgcgcca
      121 atccatatca tgcttatttt aggtggtatt gcaatggcat taccttttat ttggatgatt
      181 ttaacttcag ggaaaacgtt ggttgaatct acccaaattc cacctacaat tttccctgag
      241 aaatttcagc taagtaacta tcaacaagta cttgatttat taccattcat ggctttttat
      301 atcaatacat ttttaatggt tgttggacga gtgattggct cggttttatt ttcagcaatg
      361 gctgcatatg cttgtgcgcg tctaagtttt ccagggaaaa atatcctttt cagcttagta
      421 cttattcaaa tgatgattcc cggttccatt tttattactc cacagtattt attagcacaa
      481 aaattgggta ttttaaatac aattccagca ttaatattac caggggtagt tagtgcgttt
      541 ggtacgtttt tattaagaca atttttctta ggtttacctg atgaattgga ggaagcggcc
      601 tatttagaag gtgccaatat ttggacgatc tttttcaaaa ttatgttgcc gcttgctaaa
      661 tcaggattgg ttgcacttgg aatattcaca gcgttgtttg catttaaaga tttaatgtgg
      721 ccattgattg tcaatacttc aattgataag atgcctttag ctccgggatt agcaaactta
      781 caaggacaat atgccacgaa cttcccacaa ttaatggcag gttcgttatt agcaatttgg
      841 ccaatagttg tgatattcat cgttttccaa agaaaattca ttgaaggaat tgctacaaca
      901 ggtagtaaat tataaaacta ttaattagtg attggagagt aagtacatga caaaaattac
      961 atttattggt gcaggaagta ctatttttgc aaaaaacgta ttaggcgatg cgatgttaac
     1021 tccaagtcta caagatgcac atattgcatt gtacgatatt gatgaaaaac gactaaaaga
     1081 atcaaattta atgcttaaaa atattaataa aaattcgaat gaaaatcgtg ctgatattgt
     1141 ttcttattat gatcgcaaag aagcgttaag agaggctgat tttgttatca atgctattca
     1201 agtgggtggt tataaacctt caacagtgat tgattttgat attcctaaaa aatatgggtt
     1261 gcgacaaact attggtgaca ccatggggat tggtgggatt tttagaaccc tacggacact
     1321 accagtaatg ttagactttg ctaaagaaat ggaagaagtt tgtccaaatg cttggttatt
     1381 gaactataca aatccgatgg cgatgttaac catgggtgtt ttaaaagcga cgagtattaa
     1441 gactgttggt ttatgtcata gcgttcaagt atgcgtacca gaattgtttg agcatttagg
     1501 aatagccgat caatatgact taaatgactt tcaatggaaa attgcaggca ttaatcatat
     1561 ggcattcttg ttggaaatta caaaaaatgg ggaagatttc tatccaacaa ttcgtcagtt
     1621 agctgcagaa aaaccaaatc cacatccaga ttctgtacgt tttgaattgt tgaaacgttt
     1681 tggttactat attacagaat caagtgaaca caatgcagaa taccatccat actttattaa
     1741 atcaaaatac ccagaattga ttgacgaatt agggattccg ttggatgaat acttacgtcg
     1801 ttgtgaacaa caaattaatg attgggaaag catgcgagag gacattgtaa ataatgggga
     1861 gttaacacat acgagaagcc gtgaatatgc ttcatatatc atggattcta ttacgacagg
     1921 aactccgtca gtgattgcag gaaatgtctt aaataaaggc ttaatcacaa acttaccgac
     1981 agatgtttgt gtggaagtac cttgtttagt cgataaaaat ggcgtgcaac caacctatgt
     2041 cggtgaattg ccaacacaat tagctgcttt aaatcggacg aatatcaatg ttcaagaact
     2101 gacagtagaa gccgctttaa cactagataa atcaaaaatt tatcaagcag cgtatttaga
     2161 tccacatcta tcatctgaat tgtctttaac agatatcaaa aatttagtgg atgatttaat
     2221 cgaagcacat ggggactatt tacctgaata taattaaaaa gaagctgaaa gagtaaggaa
     2281 ttttcaattc cttactcttt tcttagttaa atttagtaaa aatattgaca aaacacttag
     2341 taaacagtta cattagtgtt aacaaaaaga aaacgcattc aaattgtata aggaggagca
     2401 taaaatgaag tggtctaaaa aaattatttt agcttgtata gcactaggag caacgatctc
     2461 tttgactgct tgtggaaaaa atcaggaaga tgcaagcggt aaggtagaaa tagagttttt
     2521 taatcaaaaa aaagaaatga ctcaaacgat tcaggagatt gctaaagatt ttgaagcaaa
     2581 aaaccctgat attcatgtaa aagttgtgga tgtaccgaat gctggtgaag taattaaaac
     2641 acgtatgctt gcaggcgatg taccagatgt cattaatctt tatcctcaaa gtatagaatt
     2701 gaaagaatgg gcgaaagctg gttatttaga ggacttaacc gaagaaccgt atttagagaa
     2761 tatcaaaaat ggatatgcac aacgttttgc tattgaggat cgtgtatata gcataccatt
     2821 aaccgctaat gtctatggtt tttactataa caaaactgct tttgaggaaa tggggattca
     2881 agcaccagaa acttgggcag aatttgaaaa aatagtagct gagattaaag atcaaaacaa
     2941 agtaccgttt gcgattgcag gagcagaagg ctggacttta aatggttatc accaactagc
     3001 tatagcgact gctgctggtg gtgaagagga agcgaacaat gtttggcgtt tctcagaagt
     3061 taatggaatt aatgctgaaa gcaaagaaat gcaaaaagat tttaatcgat tagatttgtt
     3121 aagagaacca aaagctttgc aaaacaactg gcaaggtgcc ggttataacg atacagtagt
     3181 tacttttaca aaaggtgatg cgttgattat gccgaatggt tcatgggcaa tgccgatgat
     3241 tcattcacaa aatccagatt ttgaagtagg aacttttcct tttccagcag atgaagcagg
     3301 tcaaagtcta acgattggcg caggagacct agccttatca gtttctgcaa cttctcaaca
     3361 taaagaagaa gccaaacgtt ttgtagaata tatgacaaca cctgaagcga tgcagaaata
     3421 ttatgatgta gatggtgcac cgtgtgctgt tgagggcgtt atcgagaata ctcaggattc
     3481 accattaagt ggtttaactg aacttgcttt tactgatcgt catttagttt ggttagccaa
     3541 agattggaat agtgaaaatg atttttatac cttaacaaca aattatttac acaatggcaa
     3601 tcaaaaagcc atgattaacg cattaaatgc tttctttaat ccaatgaaag ctgacggaag
     3661 gtaggaaaat aaaatgaaaa taaaagaaat tttaaatcgt tactgggcaa tcatttttgt
     3721 agcaattcca attattttgc agatgatttt tttctacttt ccaatggttc aaggtgcttt
     3781 ttacagttta actaattgga ctggtctgac ttataatttt gattttgtgg gtttgaacaa
     3841 ttataagatt cttttttctg atccgaagtt tatcaaatcg attggcttca ctttaattgt
     3901 gacgctttgc atgattgctg gacaaattgg ttttggtatt ttgattgcgc gggcgttgaa
     3961 cgcaaatatt aaagggaaaa cattctttag agcatggttc ttttttccag cggtcttatc
     4021 gggcgtggtt gtttctttag tatttaaaca aatttttaac tatggtttac caaaaattgg
     4081 tagtatgtta ggcattgaat ttttacaaac cagtctttta ggaacaacat ggggagcgat
     4141 ttttgcagta atttttgttt tactttggca aggaattgcc atgccagtca tcattttctt
     4201 agcgggtttt caaagtattc cacaagaaat tattgaagct gctagaatgg atggggcgaa
     4261 tgataagcaa attttttgga atattgaaat cacctactta ttaccaagta tttcaatggt
     4321 atttattatg gcattaaaag gtggcttaac agcatttgat caaatttttg ctttaactgg
     4381 tggtggacca agcgatgcca caacatcttt ggggttactt gtttataatt atgcttttaa
     4441 aaacaatcag tttggttatg cgaatgctat tgctttagtc ttatttatct taattgggat
     4501 tgtttcactg gcacaattaa aattatcgaa aaaatttgaa atctaggagg tttcccaatg
     4561 aaaacatcaa aagaaaaaaa tatttctaaa tacttgcttt tattattagg gggcttcatg
     4621 attttagttc ccttagtttc aacgctattc ggttcattta aaacaacacc cgatattatg
     4681 aataatttct ttcgttttcc taatccagcg actttggaaa atttccgaag attgttagcg
     4741 gatggaattg gtcattactt ttggaattcg gcaatcatca caatcattgc agtagttgct
     4801 gtgacagcaa ttattcctat ggctgcttat tcactagcaa gaaaaatgac tcgtgggaaa
     4861 gcatacggat tgatgtattt atttttaatt ttaggaattt ttgttccttt ccaagtaatt
     4921 atgattccga ttaccgttat gatgagtaaa attggtttag caaatatgtg gggattaatc
     4981 ttactctatc tcacttatgc cgtaccacag acactatttt tatatgttgg ttttattaaa
     5041 ctaaatgtgc ctatcagtct ggatgaggct gctgaaattg atggggctag tcactttaca
     5101 acttatttca aaattgtttt cccaatgtta aaaccaatgc atgcgacgac tattattctt
     5161 aatgctctat ggttttggaa tgatttcatg ctaccattat taattttaaa tcgtgattct
     5221 aatatgtgga cactaccatt gtttcaatat aattatacgg gacaatattt taacgactat
     5281 ggtccaagtt ttgcttccta tacagtagga attgtcacga ttacactagt ttatcttgct
     5341 ttccaaagaa gtattatagc tggaatgagc aatggagcag tgaaataacc aataaattat
     5401 tctcattata aaggagttca taaacttatg attatatttg acaatcacac acaaacattt
     5461 catttacaaa ataaaaaaat tagctatcta ttaggaatcg aagaaaataa ttatttaacg
     5521 catctttatt ttggaaaaaa gattacgcac tattctggtg ggtatcgcta cccccgaaca
     5581 gatcgttcct tctcacccaa caatgcaaat gcgcaagatc gtttattatc tttagacaca
     5641 ttaccattag aatttccagg atatggacat ggtgatttta gagaaccggc gcataatatt
     5701 aaattaggaa acggtagtcg tgtaaatgat tttcgttatg atagctacga aattcaaaaa
     5761 ggaaagccca gcttagcggg attgccagcc ttatatacgg aagatgatga tgaagctgaa
     5821 acgctgatta ttcaattgaa agacgcgatt agtggattga ctttacagtt gttctacaca
     5881 atttatcaaa atcacagtgt aattattcgt tctacgcaat tagtaaataa tagtgatgaa
     5941 acagtggaga ttaaccgctt agctagtcaa tcaattgatt ttccaaatcg tccactcgag
     6001 ttaattcatt taaatggtac ctgggcaaga gaacgtcaaa tgactagaga aaaaattcat
     6061 acagggataa aagtattaga tagtaaacgc ggatcaagca gccatcaaca aaatccgttt
     6121 gttgctttag tagatgccaa aacagatgaa tttcaaggag aggcatatgg atttaattta
     6181 gtttatagcg gtaatcacga aacggtgatt caaaaagatc catttgacca aacacgggtc
     6241 attatgggaa ttaattcatt taattttggt tggcaactaa aaactggcga ctcatttcaa
     6301 acgccagaag tcgtgatggt ttatagtaat gaaggtctga atacgttatc acagacgtat
     6361 catgaattgt ataatcaccg tttaattcgt ggaaactatc gtttaaaaga acgtcccacg
     6421 ttaatcaaca actgggaagc aacgtatttt gattttaacg aagaaaaaat catgaatatt
     6481 gtcgatgatg cgaagaactt gggcattgaa atgtttgttt tagatgatgg ttggtttggc
     6541 ggtcgtgaaa atgacttcac ttcattaggt gattgggttg aaacagaagg caaacttgct
     6601 tgtggtttgg aaaatttagc caagcaagtg catgataacg ggatgaaatt tgggatttgg
     6661 tttgaaccgg aaatgatttc tgaagatagt gaactatttc gagcacatcc agattgggca
     6721 ttgggtattc caggtcgtga ccgctcaatt agtcgaaatc aatatgttct tgatttttca
     6781 agacaagatg ttcgtgataa tatctatcaa caaatgacgg cgatttttga tcgtgtaccg
     6841 attgattatg tgaagtggga tatgaatcga aatatgacag aagtctattc tgtcttgttg
     6901 gactcaacga tgcaaggtga agtggctcat cgttatatgt tagggttata tgaattttta
     6961 gaaaagttaa caacgaatta tccaactatt ttatttgaaa gttgttcggg tggaggcggt
     7021 cgttttgatg caggtttctt gtattacatg ccacaaactt ggacgagcga caataccgat
     7081 gccgtggctc gtctgaaaat tcaatatgga accagcttag tgtatccaat ttcaagtatg
     7141 ggtgcgcatg tttcagcagt cccaaatcat caaacaggaa gagaaacgag tttaactatt
     7201 cgtggcaatg ctgcgatggc tggtgttttt ggttatgagc tagatctaac gcaattaact
     7261 tctgaagaaa aagcagaaat taaagaacaa gttgcctttt ataaaaagca tcgccaactt
     7321 ttgcaatatg gaacttttta tcgcttggaa agtccgtttg aaggcaatga tactgcatgg
     7381 atgtttgtct caaaagacca aacagaagcc atggtctttt atttccgagt attagcagaa
     7441 gctgcgcatc cattagtcac attgaaatta gcaggattag cagaagaagc aacttatcaa
     7501 atggaagatg taacatttgc tggcgatgaa ttgatgaatc tcggttttta tattgactca
     7561 gaactacatg gtgattatgc gacacaacgc ttttatttga aacagatata gtagcttgaa
     7621 tttacaatat aagtgcaaca ccaaaagaga ctcattccaa acgaagaaaa tatgttttat
     7681 ttacacgcat ttactaaaaa gcagcctgat ttgaattggg aaaatgaaga agtacgtgaa
     7741 gaaatctatg caatgattaa ctattggtta gataaaggac taggcggatt tagaatcgat
     7801 gcgatattaa acatcaaaaa aaaactagag tatggcatct ttgaatctga tggagaagat
     7861 gggttagcct tcattgggcc ctggattctt aatcagtcgg gcattgaagt gtggttaaaa
     7921 gaaatgagag agcggacatt taaaccacat aatagtatga cggtagcaga agccgatgtt
     7981 cctaatgaac gtttagatga atatattggt gaagatggct attattcgat ggtttttgat
     8041 tttagttata cggatattga tgtacctgaa actggtgaat ggtttaaaga tagccaatgg
     8101 acatggacgg atatgcgtac caatattttt acgaatcaat tagttacgca agataaaggt
     8161 tggggcgcct tatatttaga aaaccacgat caaccacgtt caattaataa atatattcca
     8221 gaagaatgga tcaatgatta tagtaaaaaa atgttggcga cgctttttat gttactaaga
     8281 ggtacccctt ttatttatca aggacaagaa ctaggaatga ccaacataga aatggcatca
     8341 ttagaagatt ttgatgatgt agctacgcat tctcagtata aacgggcatt agaatatgga
     8401 ttggcaccag agcaagcatt gaaagctgtc aataaacgaa gtcgggataa ttctcgcaca
     8461 cctatgcaat ggaatacgca aaaaaatgcc agattctcaa cttctgataa tgtatggtta
     8521 aaagtaaatc ctaattatcc agaactcaat gcagaagctc agttaaccaa tcaacagtcg
     8581 gtattcaatt attatcatca attaattaaa ttaagaaaaa atagtgtata caaagacgtg
     8641 ctaatctacg gacaatttct accagtgaaa gataaaaatg aacatatttt gctttatgaa
     8701 cgccaattag aagataaaat cgtattagtt atcttaaacg ttacagcaac agaacaagaa
     8761 tattatgtag atgaacagta ccaacaagta ttaatcaata attatgaaga tgttttatta
     8821 gatcaaagtc gaaaattgcg tttacgaccg tttgaaagta ttgtattagc taattatgga
     8881 ggacaattat gaaaattaaa aacgaagcaa tgctcatcac ctatgcagat agtttaggaa
     8941 aaaacatggg cgaattaaaa caagtcttag acaaacattt acaaggtgtt attggtggtg
     9001 tacatctgtt gccttttttt ccatcgacag gtgatcgagg ctttgcgccg agtgattata
     9061 cgacagttga tccagctctt ggaacatggg aagatgtgga agcattaggt gaagattatt
     9121 atttgatgtt tgattttatg attaatcaca tttctcgtga atcaaaattt ttccaagatt
     9181 tcaaaaaaaa ccatgagcag tcaccataca aagaaatgtt tatccgtatt catgaatttt
     9241 tcccagaaaa tcgaccaaca caagaagata ttgatttaat ctacaagcgt aaagacaaag
     9301 cgccgtttca aacggtagaa tttgcggatg gtacgacaga agaggtttgg aatacctttg
     9361 gggaagaaca aattgattta gatgtgacca aagaagttgt gaaagaattt attcgagaaa
     9421 ccatcaaaga tatggcgaac catggctgtt ctttaattcg attagatgcg tttgcttatg
     9481 cgattaaaaa attagatacc aatgatttct tcgtggaacc agatatttgg gaacttttag
     9541 acgaagtgcg tcaagaagca gcgaaatatg acgtcgaatt attaccagaa atccatgagc
     9601 attattccat tcaaatgaaa attgccaatc atgattatta tatctatgac ttcgccttgc
     9661 caatggtcac tctgtattca ctttatagtg gaaaatcaga acgtttagcc aattggttga
     9721 aaatgagtcc gatgaagcag tttacaacat tggatacgca tgatggaatt ggtgttgtgg
     9781 acgctcgtga tttattaaca gatgaagaat tggattacac ctctgaggaa ttgtataaag
     9841 tcggggcgaa tgtgaagaag gtctattcaa gtgccaatta caataattta gatatttatc
     9901 aaattaacag cacctattat agtgcattag gcgataatga ccgtagctat ctgttagcac
     9961 gtgcgattca agtatttgcg ccagggattc cacaaattta ttacgttggg ttattagcag
    10021 gaaaaaatga tattgaactg ttggaagcaa cgaaagaagg gcgtaatatt aatcgtcatt
    10081 actatgattt agccgaaatt gaagaagaag tgcaacggcc agtgattcaa caattatttg
    10141 cacttttaaa atttagaaat gaatctgcgg cgtttgattt ggatggaacg attgaagtag
    10201 caacgccatc agaatcagaa atagtgatta cacgtaaaaa taaaaacggt agtgatagtg
    10261 caacattaac ggctaacttg gatacgaaag aatttatcat tgagtctaaa gatataaaaa
    10321 ttttcactag tgttgcatta aaattttaac ctattccata gaacaaaaaa tcctttatac
    10381 tagcacttgg atgacttatc caagaccaat aaaaaggact catacatgga taagtataaa
    10441 acaaatacag catttaataa atggttttct gccataaaat taggaaaact ttctgtcaac
    10501 tcccaaaaat gtatttttga ttacaattcg tatcgtaaaa aactgtcttt tgaagctgtg
    10561 ctcaagctct ttctctacgc agttaatgaa gaaaaagaaa gtttgcgtga tttgagtaca
    10621 gctcttatcg accgatcgct ccaactagaa accgatgtgt catcgattag tcatacccaa
    10681 ttgtctcgag catttaatgc acttgaacct aaagtgttag aagaaatttt tcaacaactg
    10741 ttagaaaaag ttcaacatca aatcaaacca accaagcgaa atcatctgta tctgattgat
    10801 tccagtacgt tttcgcttag tttaaaacgt cataagtggg cgactttccg tcaaacaaaa
    10861 tcaggtgtca agttacactt aaactattgt tatatggacg atactacgat gtatccaact
    10921 gattttacct taacaaatgc ccatgaacat gatctcaatc aactaccagt attggtgaat
    10981 caaccagagg caacttacgt gtttgatcgt gggtattagg actttgaaaa gatggatcag
    11041 atgcattggg atggctattt cttcgtcact cggattaaaa aaaacaccaa agttcacgtg
    11101 atagatactc tggaaacatc gccagaaaca gaaattctac gagacgagct ggttcgttta
    11161 ggatctaaaa cctatctaac cgccaatttc cgactagtca ctgttcaaga taagaacgga
    11221 cgtgtgtttc aatttatcac caatcgaatg gacgtctctt caaaagaaat ctctgatatg
    11281 tatcacgctc gttggcaaat cgaattgttt ttcaagcaca tcaaacaaca catgaccatt
    11341 aaaaccttct tttctcaaag tgaaaaaggc gtgcagaatc aattgatttt aacgatgatt
    11401 tccgctctcc tcacgtttct catcaaattg gagacgaaaa cggaaaaatc agtgttccaa
    11461 atcaaacgat tctttcgtta tttactgttt cagccctttg aatgttgtct agaaaaatta
    11521 attcccacat gagaaaaaag aaatttggct cgttactttt ggttttcagt tgaataacag
    11581 ataaaaaatt ttctggaaaa agtcatcctc tgcgtcagca cagttttttt tgacttattt
    11641 tcagaggaaa tagttttcag aatattctaa aatgttttat gcaacactag tgatattttt
    11701 tagaattaga t
//
LOCUS       DAHHZJ010000087        11687 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.088, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000087 DAHHZJ010000000
VERSION     DAHHZJ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.088"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(<1..400)
                     /locus_tag="NBL02_001786"
     CDS             complement(<1..400)
                     /locus_tag="NBL02_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572468.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLN"
     gene            complement(393..794)
                     /locus_tag="NBL02_001787"
     CDS             complement(393..794)
                     /locus_tag="NBL02_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572469.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            complement(784..1137)
                     /locus_tag="NBL02_001788"
     CDS             complement(784..1137)
                     /locus_tag="NBL02_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCD2572470.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            complement(1127..1438)
                     /locus_tag="NBL02_001789"
     CDS             complement(1127..1438)
                     /locus_tag="NBL02_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572471.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            complement(1435..2262)
                     /locus_tag="NBL02_001790"
     CDS             complement(1435..2262)
                     /locus_tag="NBL02_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572472.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLSFVNKKKCSYAYVKAIKAVVR"
     gene            complement(2272..3432)
                     /locus_tag="NBL02_001791"
     CDS             complement(2272..3432)
                     /locus_tag="NBL02_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCD2572473.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            complement(3432..4118)
                     /locus_tag="NBL02_001792"
     CDS             complement(3432..4118)
                     /locus_tag="NBL02_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCD2572474.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            complement(4081..5259)
                     /locus_tag="NBL02_001793"
     CDS             complement(4081..5259)
                     /locus_tag="NBL02_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCD2572475.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            complement(5279..6973)
                     /locus_tag="NBL02_001794"
     CDS             complement(5279..6973)
                     /locus_tag="NBL02_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCD2572476.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            complement(6951..7265)
                     /locus_tag="NBL02_001795"
     CDS             complement(6951..7265)
                     /locus_tag="NBL02_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCD2572477.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            complement(7368..7649)
                     /locus_tag="NBL02_001796"
     CDS             complement(7368..7649)
                     /locus_tag="NBL02_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572478.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            complement(7654..7998)
                     /locus_tag="NBL02_001797"
     CDS             complement(7654..7998)
                     /locus_tag="NBL02_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCD2572479.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            complement(8024..8191)
                     /locus_tag="NBL02_001798"
     CDS             complement(8024..8191)
                     /locus_tag="NBL02_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572480.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKENNEA
                     IPLYGKRIAKH"
     gene            complement(9185..9652)
                     /locus_tag="NBL02_001799"
     CDS             complement(9185..9652)
                     /locus_tag="NBL02_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HCD2572481.1"
                     /translation="MVLFDVKKYETPEAKDVDMDRTKHNVGVFLSAYLSARCRIGQPR
                     EPKVTASYSLVPSSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKLESNKIIIQFASSLELVAFK"
     gene            complement(10244..10531)
                     /locus_tag="NBL02_001800"
     CDS             complement(10244..10531)
                     /locus_tag="NBL02_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572482.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKEPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAVIRIENTMDCDKELAKSKANIAVVRLVDMKAI"
     gene            complement(10531..10725)
                     /locus_tag="NBL02_001801"
     CDS             complement(10531..10725)
                     /locus_tag="NBL02_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572483.1"
                     /translation="MKAYTIKFYGNKTFVYILSGLRLDFRGYSKDDLLESLYGYGYLN
                     EDELISLEKFTNAWTIEEEA"
     gene            complement(10728..11114)
                     /locus_tag="NBL02_001802"
     CDS             complement(10728..11114)
                     /locus_tag="NBL02_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572484.1"
                     /translation="MKSLQQLISEKESELQNLEDSLGLGFPIVEQAKMTQISHLRLES
                     EDLRQIEKSIQLNDNQQIVFDFLKNEFKRNDLQYTLWSFTEDVYERLEIGVSSLPYVK
                     AWGKLKEKQQFEVLTAFAQWGLEQEE"
     gene            complement(11111..11650)
                     /locus_tag="NBL02_001803"
     CDS             complement(11111..11650)
                     /locus_tag="NBL02_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HCD2572485.1"
                     /translation="MNKQELIDKLASLVGSIEDFKGISTFHDGKYAGLEHALELIKQL
                     DEPQKPFVPKFVADYIEEYRGGGATLYEMMDSSEDEVYTWLFGNPRIKTENERQLLFA
                     QAYAVGYEVEKEPLYEVIIGNLFLVKKFNNRNDSCFVNKFELCWWEKSAYQLTEPEIK
                     AIDERYWPFAVPVEEVAEG"
BASE COUNT         3348 a         2430 c         1727 g         4182 t
ORIGIN      
        1 tatttaattt atcgaattga cgaatcagaa gttctgtaat tggatcaagt gcaagaccta
       61 atcctcttct cataaattcg tccggctgat ttcttttaga agtacctatc cccaaatcag
      121 gatattttaa atactcaaat tttcttgtag gtctaatgat gaaacccaaa ttaatgtatt
      181 gagtcttaag tggacgacta ttttttgcgt gttggtgccc tcttcttaaa tctgcttcag
      241 aaacaggaat tttttctgta atcctatcca ctgcaatagc cgaacctttt gatttcaatg
      301 cttcgttaat cagtcgttcg ctctcgcttg aatagcgttc catccgcaca agaagttcat
      361 catgtccatt tatttttagc tcccaactat ttttagccat gacaatcact cttcaataat
      421 cgtctaaacg taaataccaa ttgatcgata tagcgatctt ggttctctag ttttaaatga
      481 ttgggatcca ttctctgaaa acgaatcgaa cgattttgaa tcaatgaaat aatatctagt
      541 gaatctcctg ttaaatcttc tctattttct gaatagaaag ttagatatag attttgaccc
      601 acgctatatt ttggctcagt gatcatttct atttctcctg tttcgagaat gaagtaatta
      661 aaatcatcag gtagctcatc ctcgcctacg gagtcttgaa agagtttgag gccaaaatgc
      721 tcttctaagg aagttttgat agcagaaatt tgcttattta aacgttcttt ttctttagaa
      781 ttatcaatca ccatattcac ccacactttc aagataaaaa tagatataaa aattatcgta
      841 atcggcatag ataacgttgt aacgcatact atcgattacg ataaaatatt gatctttatt
      901 aaatttcttg gcgattggat gaaatggagt ctttactttc ttagttaatt tcgatcccat
      961 cgcatccata gctgttatat cactatctct catggaaaga tttctaaatt ttaaagaagt
     1021 gatttctgta tcttctacac caatcttttt tcctagttca tttcttttgg tagtttgcgt
     1081 caaaatcttt aaccaaccat cgttgaatgt ttcttcgagt ctacgattat tcgccattca
     1141 catcacctgc aatatattct tgtagcgcat aatgttgaat gaaacctaat aactcactag
     1201 cgaaattttg ttcaaactca tctaaagcac gattccagtc gtatctacat ctttcgatta
     1261 gcaatccgta ttctaagctt tcaggagaaa aagaaagtgt tgtactcact ttactttgaa
     1321 gataaacagc atttttagct atcatctttt taattgactc atcttcttcg ttccaggtaa
     1381 cgtaaatatt atccttcaca gctattagca attcttcagt cacttgttca ggcgtcatct
     1441 aaccaccgcc ttaattgctt taacatatgc gtaagagcat tttttcttgt ttacaaatga
     1501 taaatcttca tcgtttgttg ttactccagc attgtgtaag atttttactt ctttaacttt
     1561 ttctattgga tcagtagcaa aacaaaagtc taattcctcg tgaactttag gaccaatatt
     1621 gaaatacatc atgttccaaa gctgtgccca catctcggct gtccagattt gtatatttgt
     1681 tttttgccct ctaaggtagc gatatagccg attagaatcc agataaacct ttttccaata
     1741 attcgcttta gggcggttaa taacccactg tgcgcctcct gaattagtgt ttatagtttc
     1801 caaagattct actgtaacat ttacaatgtt tgccatatct tttagaatat tttctccgtt
     1861 ttcacagctt ctaatataat caagacttag ataactacag cagtcgctac aataccaaac
     1921 atcatcttta gaaggcaatt tgcgcaaatt aattctttta ttgaaaatga catccgaatc
     1981 gatatagaaa tatcggtcgt cctcacgcga atgatcttct tctaaatatt tccaccataa
     2041 atatggttta atcgaaggaa tatactcttt gtcgtcccgc agatcatcgt acacatgaac
     2101 ttcaacacca tattccttct caaaaaaaat aggaatctga tcatcgtgtc tgctgaaaag
     2161 caatacgata tctttgattc ctagtttctt cagattagtt aaacaaactt caagctccca
     2221 tttaaaccga ttgattgccg gctgacaaag aatatacttc attctgatca cctacgcttg
     2281 tgttgtagtt gttgtggttg ttggttttgt agttgtagta gtagttccca aagcgctaat
     2341 atctaataca atgaaactat cgttacgttt aggttgaccg tttgcatatt gtttagctag
     2401 ataaatgcgt tcgtcttcaa caaaatggta ttcatctgaa gcttcaattt ttagtgtaga
     2461 tcctacaccc atgaagtaat ctgaggctac cccaataact gcttttcctt ctggcacagc
     2521 cgttgactgc aaatctgaaa ctggtactgg caatacttgt acgtattctc cattagcagt
     2581 tagtacagtc ttagctggga atactttaga ccagtaatca gttggattca caattaggac
     2641 cacatcagaa ggattcacat tacgataaat cggatcattc acaccttcga tattgaattt
     2701 tgatagtcgc gccatcaaac cgcccatagt tacagcatct aaagctgtaa taggttctgc
     2761 ttttttttca gcatattctc cgctagtttg tttgctcatg tcacgcatca ttccgactgg
     2821 catatcttta ccagtaccat caacaattgc ttgttctaat gcaattctca atgattctac
     2881 taaaacagta cggacataac gatctaacca tactggacct aaatcaagca ttgccttaca
     2941 tacaggaata taacctgata gcttgaactg cttcatgtta attacatcaa agccattatc
     3001 taaaactttt ttaacagctt cgcaaagttt accccaccat gctggattga ctccacgtga
     3061 cacaatccat tctgttacac cagttgtgtt aacaaaagta attttttgca atagtggatg
     3121 agattgttct aaatcttcaa atacacgttc aaatacagta gctggcacta attcttcgac
     3181 ccctgcaaaa ccttcgtttt tcactacttc gttatagaat tttgtttctt gtgtagttaa
     3241 tacacgctga ccacggttca ttaatactaa ttgatcttga ttttttgctg ttgcttcttc
     3301 taaaatttta tcctgaattt ccttagataa gcttaccata gctgcgctaa aagattcttc
     3361 gttaccatct ttaaaagctt tcatcaattg gtcgcttgca gctgttacac cttttaaatt
     3421 tttaactgtc attattttac atctccttgt ccaaatgttt tatttaatgc tgctgtaaat
     3481 gcagcaattt tttctgctct tttttcttta acgtcattca aaatttcttc aacgctttgt
     3541 tcttttttag cttcagtacc tgagctattt tctgcatcga taatttcatc gaccaatcca
     3601 taactcaaag ctgtttctgc atccataaac gattcttttt caagaagttc ttgcaatgct
     3661 tcatctgtgc cattgaatcg tgttttatat gaagccttta ccgatttatc aattgattcc
     3721 agttggtcag caatcgtacg gaagtcatcg acatttcctt ctccgtatgt ggaagcgcgg
     3781 tgaatcatca attgtgcatt gttgtagatt tttatagtat cgccagccat tgcgataatt
     3841 gaagcagcac tagcggctaa gccgttaatc acaacgttaa cttttgcttt atttgactta
     3901 agtaagttcc caatagcaat cccttgaaat acgtctccac cgtttgaatt aattactact
     3961 tcaatttctt cttgatcacc tagactatcc aaaatatttt tgattccctt gtcagtattc
     4021 ccttcaaaga accaactaga accaataaat ccctgaataa aaatttgcgg tactgcgcct
     4081 tcattcttta ctgctagaaa tgttttcatt gtcgtcattc gcctcacctc ctttcgatac
     4141 ttgttgattg tttttagtta taaatatttc atctgccatc gccttatcag agcgatcatt
     4201 tccaacgcgc tctcttcctt cgttgattgt aaatactcca tttctaatgc ctacatcaat
     4261 agcgtcaacc aaatctttga agctagtaat cttgatcata gttgtatcca cacgtacaaa
     4321 attccctgac aagtattctt ctacttcata gagactagcg ttaaacgcat cctgaataag
     4381 ttcagcaatc ggtatgattt cgaacattaa aaaagcgtcc acttgatccg ataacccact
     4441 catgtctccc tttagtaggt ttttcggaac gtgaaacgct gctgctgtca tctcaaagat
     4501 gtcgtctatt aagtttttta tatctcttga attgctttgg aagtttccgc tgaaatcttc
     4561 taatgtgtac tcattttgta attgaaatac cgcacctgca ttatcagctt ccataaaagc
     4621 cttaaattgt gatgtcatca ttttattgat ttgatcttgt gttgtattgt cttgcggtcg
     4681 gaataaattc cctttcagta cgtatctacg agcgttagag cgcttgtaaa cattcatggc
     4741 actagaaatg agtttcccat acgcttgata atacgcatcg actagttgcc taatttgttg
     4801 atctgcgtat tttatataga taacatcact ttctagaaat tctctatcaa ggactatgtt
     4861 gttaatttgc acttgagaaa acacatcatc tttcaatgca tattctgtga catcccaact
     4921 atccgcaata aatatttcgc tagaattatt agacggagaa acgatcaata cttcattgta
     4981 gaatattaat ctcctgatca gtttttttct aaattctgtt gcattatttt tcttattagg
     5041 agctacattc agcctatagt aaagatcatt ctttttattt tttccatctt catatgactt
     5101 gaattccgct ttactcatcg catttgcaat caaatcaata caagtttcaa tcgcaaattt
     5161 tcgatacaca aaatcaactt gcaatttaca aaagtattct tctaaaggaa ccgttgcttt
     5221 ttttgtgaag tatcctaccg ccttttgaaa aatccccact ttctcacctc ctttcaagtt
     5281 agaatactag aggagtaaat ccagttcctg tattttctac tgagctattt gtgactgtta
     5341 caggagcaga atcataaata tcatctaaaa aattcaaacc atgaaggaat gaaaaaaagc
     5401 catccgtttt tctagtttca ggttctattt tttcatagcg tatatttcca ttagaaatat
     5461 gctcttcata tacattcatg caataccaac gcataatcgc atcgtcacca aaaaataaac
     5521 gttgattaat aaaaaggtca tcaaccagat cttttaacat accatgtgta acagatccgc
     5581 ttcgaacaat ttccacagta aaacctgctt cttctaaagc gggcttcaat atttttgcac
     5641 ggtacatatc catagcgatt tttttaatat aatatttatt actcatttca agaaaccaac
     5701 ctacaatata atcagcttct atattttttc catgaacgat ctgtgatttt ccttgatcta
     5761 tagaaatatc tataacctct cgtttgatgt tttgtaatcg aagggctgat tcgtggataa
     5821 aagtatgttg tgtaaaataa acatctttat cgtattttcc tagcaaccca acgctggcaa
     5881 aatctcgtct atcagcaaaa tcgactgttc ctatcacttc atccattttt tcaggaaatt
     5941 ctttttcttt cgtatgcaga acatcatcat atgaagcaac agcaaatcgt gtatcttcca
     6001 taggtctgtt cattcgtttg gtcatgaacg taagtcttaa accagcatta cgttgcattt
     6061 gagagtattc ttgaaacatt ttccgtttta aatctgcatt gtaattaata gttggacaag
     6121 ctttttccca catgtcggga tcatcaactt cattatcgtt atccaagcga caaataaatg
     6181 gaaacaaact agaaaattct gctccatcct tgtcaattcc aagttctcca gaaagaatca
     6241 tttttgattc ttctataatg tcatcaagcg gaccaccacg aacatgacca ttagttgtat
     6301 cataaaattc tctataatct cgaattttac caccaccaga agtagccaca tttatcattg
     6361 aataatcttc attttcgtga atttcatcaa agcggtttgc acctggtcgc ttcccatctt
     6421 ttgttctagc atttgccgtg ttataacgaa gtttgctgtt tgtagcgata ttttgaataa
     6481 cttccttcgt agctttaaat acttttttat ctaaatcagg atgatcttta attactttaa
     6541 atacatcatc aaaactagtc tttgcttggc tttcattatt ggcatagata tcaatatcat
     6601 aatttttaat accgtgttta gcggttagta gaaagaagtt gttccaagaa gcaaaaccag
     6661 ttttaccatt accacgtccc attaatgaaa gatatctatt gaacactagt gttttatctt
     6721 ttttccatcg aacaccataa ataaaacatt gtagaaattt ttcccacgga attaattcga
     6781 atggaaagta ttgtgctggt atattgattg aatcctctac catctgctta tcgaagtaaa
     6841 tatcttctct agtaaagact ctttcttcta gataattttt tagcaataat tgctctttgc
     6901 ataccttgat agtgccttct tctatagctt tgaaccaatt ttcaatatgc ttataactca
     6961 ggaattgatt catttgcttc acctaccaat tcaggagtaa tggcaagttt atccaacatc
     7021 aatcccattt gtttgttgac agaaacaagc aacgctactg attcattctt tttaccattc
     7081 tccagtctaa tgccgttctc ggatatatct tcttccagtg atatcgccgt ttcccataaa
     7141 ctgatataac gatcaacatt atctaagaat ggctcaatat ttgttttctg actttccaat
     7201 tggcttatta aagagcggcg taatttttct ctgtagcgat tttgagacaa ttcgttttta
     7261 aacattttag ccctcctttc atgataaagt tcgaaaaaat ctcttttcct gacagccccc
     7321 tccgtttcat cacccccaaa aaatttgcga tttattttaa gggggggtta tctcaccatc
     7381 ggaatgaaag cttcagcgaa gtcaatgtaa taattaatct cttcaatgct atatccaaaa
     7441 acatttttta ttctttcaac gttattatct ttattcaacg cttctcttac ttgattcact
     7501 ttgtatttac tacaacagtt atctgataac agatccctaa tacctacata gcgaacgtat
     7561 atcaaacgtt taatcaatcc ttgtgtataa gatgaatact cttcaatctt ctctgtgtca
     7621 tactctctgc cattgtcatt gatgatcatg cacttaccac ctttcacttg catcgaagtt
     7681 agcgaagctt tctatcttct tctcttgttt atccaatgct gtaagatatc tgccatgaac
     7741 ttcattatga tgttcaacac ataaacaaat aagattatct aaatctaaag ctaagtcagg
     7801 tctatccttg acttccttta tatgatgaac gttctctact ctatgatact tacctagtct
     7861 tctacactct tggcattcat agtgatctcg tttcatcgct ttctccctaa gcctgcgcca
     7921 tttaggagac tgatagaact taaccaaacg atcttctctt atcaactgta atagccatct
     7981 atagaattcc tcagtcatga cccgtctcct ttcgcaatct tatttaatgc ttagctattc
     8041 ttttgccata caacggaata gcttcattgt tttcctttcg tttatatgta tcactcttta
     8101 ttggtcttct atactttcgt actatctcac cgttaccatt ttgcacagtg attacttcat
     8161 acttctgttc taagtattgt ggtctataca ttgttgttac ctcctttgtg caaaaataaa
     8221 aagaccactc aacgagtgat ctcatatgta atgcacaggc agggacgttt ccgatcctgt
     8281 gcttgagtca tttgacgatt catttgtacc aaatcccaaa actcaatcta acctaaccaa
     8341 ctatgtaata gcaacctaca ccgattccat cgattactat cgacctcgcc ttgctcgtgt
     8401 actttgagcg cccatttcca accctcagtt gctaatgaat actgaaacag aataccgtct
     8461 ctgttcatga ccaacgcgac agtacaaagc tttgtcgcaa gcaggttatc gtattcctaa
     8521 taaccccaac taatatttct ctcctaacct acacctgaga gtggcgcacg tctgcgctag
     8581 tattttatgc cttttaactg ctagtggcat cccgactagt cagccaacca atcggcatcc
     8641 gataagattg gttgtttccg taggttcctt aagtcactgg caatgaatcg aacattgcat
     8701 ggttgccgaa gcattgacct agcacgcatg cttagcgtct accctttccg ccacagtgac
     8761 actataaaat tattcttggc tgctactatt ttttattttg cccattttta aatccaatca
     8821 tatagacatt aagacagagc gcaaaaattg aaattattaa tggaatcatt tctcttcact
     8881 tacctttagt tatcgtgtga ataattaaaa aaacaataga cagcaacgga tgatagataa
     8941 taagaataat ttagaaggag ttgaaattca catccttatt cttaatattt ccgctgctgt
     9001 ctatcgaagc ttaattaaac gatgagggag atttcctccc ttacatttta ttttgtctca
     9061 gacctatcac taatctttcg acactatcat aatacaacat tgaataggta agtgattggt
     9121 ataaaaaggg tataaaatgg aaaccaaatg ggtaataaaa gggtataaaa aatgtaaaaa
     9181 ctggctactt gaaagcaacc agttctaacg atgaagcaaa ttgaatgata atcttgtttg
     9241 attctagctt gacagattct tcactcgtgt tatttctttg agcagttaca taaatgggca
     9301 gaccattgat ataacgatca tagaatatct tcttgcgcct ttcagtcaca tcaggcttat
     9361 gcggatgctg tatcgctgaa tagcctcgaa caaacaattt atgcagatac tcaaattctt
     9421 cttgtgcttc ttctttatcg atcagcattc tttctgcttc aaatatatga tcagctgtag
     9481 aagatggaac caaggaataa gatgctgtca cttttggttc tcgaggttga cctatcctac
     9541 atctagctga cagatatgct gaaagaaaaa caccaacatt atgttttgtg cgatccatat
     9601 ccacatcttt tgcctctggt gtctcatact tctttacatc gaaaagtacc atccattgat
     9661 tcctccattt atgatataat aattgtgtca gaattattaa ttaaggtcgg aggaatccgg
     9721 ctttttttat acccatgctt atgctaagct tttcaagtag cgaggttgca ctcgttaccc
     9781 atatacatgt tgagctatct ggcggaaaac agatggctca ctatttcaat attctgctaa
     9841 ggacagccag tggtcggctg tcttttttat attttaatga gaagccttac ttattttttt
     9901 atctttatta cgaaaaatga tattatctat tgataataaa gcgtatagga gatggttgag
     9961 tgactatgtg gagcatgctg ttattttgga ttcccgtttg tattggtatc gtcgcatttt
    10021 gttactttgt caaacactct agaacaaata agctcctcat gttatctttt ttacctatag
    10081 tattttttat tgtacaaatt gttaaatata cctatattga atcgcaagaa atattcattt
    10141 tttatgtggt aggtttattt atctctgtgg tctttttcat aatgatactt tcctattttt
    10201 ataaaaaata aatttttctc ttagaagtac attttttcgc tgtttatata gccttcatat
    10261 ccactaatcg aacaactgca atattcgctt tgctcttcgc tagctctttg tcacagtcca
    10321 tcgtattctc aatacgaata actgctgaat gattgtagac gtgttctaca tatccacgaa
    10381 atggatagat gaatccttct gcttcgcagc gaaccatgtc accgactttg aattttggtt
    10441 ccttacgtgt tttagggttc tttgtcggca tatctagcat taaaccgccg atgccgtggc
    10501 tactagcgta aaatccgtct tttagtttca ttatgcttcc tcctcaatcg tccaagcatt
    10561 tgtaaatttc tctaagctta ttagttcatc ttcatttaaa tatccgtaac catataagga
    10621 ttctaaaagg tcgtctttag aatatcctct aaagtcaagt ctgagaccgc ttaggatgta
    10681 tacaaaggtt ttatttccat aaaacttgat tgtgtatgct ttcatttcta ttcctcctgt
    10741 tctaatcccc attgagcgaa tgctgttaga acttcgaatt gctgtttttc tttcagtttg
    10801 ccccatgctt ttacataagg taacgagcta acgcctattt ctaatctttc atagacatct
    10861 tctgtgaaac tccacaacgt atattgaaga tcatttcttt taaattcatt ttttagaaaa
    10921 tcaaacacga tttgctggtt gtcgttaagt tgaattgatt tttctatttg tcttaaatct
    10981 tccgattcaa gtcgtagatg actgatttga gtcattttag cttgctcaac aattggaaac
    11041 cccaatccca aactatcttc taaattttgg agttcgcttt ctttttcaga tatgagttgt
    11101 tgcaaacttt tcatccttcc gccacctctt ccactggcac agcaaacggc cagtatcttt
    11161 catcaattgc tttgatttct ggctctgtta gctgataagc agatttttcc caccaacata
    11221 attcaaattt attaacaaaa caggaatcat ttctgttatt aaacttcttg acaagaaata
    11281 aattcccaat gataacttca tacaaaggct ctttctcgac ctcgtagcct acagcgtatg
    11341 cttgagcaaa taacagttgt cgttcatttt ctgttttgat tcgaggattc ccaaaaagcc
    11401 atgtataaac ttcgtcttct gaactgtcca tcatttcata taaagttgct cccccgcctc
    11461 tgtattcttc gatataatcc gccacaaact tcggcacaaa tggcttctgc ggttcgtcta
    11521 gttgtttgat tagttctaat gcgtgttcta aacccgcata ttttccatca tgaaaagtac
    11581 ttataccttt aaagtcttct atgctaccta caaggcttgc tagtttatca atcaattcct
    11641 gtttattcat cgctgtcctc catgtattcg tctaatatct ctttata
//
LOCUS       DAHHZJ010000088        11592 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.089, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000088 DAHHZJ010000000
VERSION     DAHHZJ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.089"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(323..2332)
                     /gene="metG"
                     /locus_tag="NBL02_001804"
     CDS             complement(323..2332)
                     /gene="metG"
                     /locus_tag="NBL02_001804"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCD2572486.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            2600..2878
                     /locus_tag="NBL02_001805"
     CDS             2600..2878
                     /locus_tag="NBL02_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572487.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            2975..3796
                     /locus_tag="NBL02_001806"
     CDS             2975..3796
                     /locus_tag="NBL02_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCD2572488.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(4081..5091)
                     /locus_tag="NBL02_001807"
     CDS             complement(4081..5091)
                     /locus_tag="NBL02_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCD2572489.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(5103..6056)
                     /locus_tag="NBL02_001808"
     CDS             complement(5103..6056)
                     /locus_tag="NBL02_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD2572490.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(6046..7116)
                     /locus_tag="NBL02_001809"
     CDS             complement(6046..7116)
                     /locus_tag="NBL02_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2572491.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(7122..8165)
                     /locus_tag="NBL02_001810"
     CDS             complement(7122..8165)
                     /locus_tag="NBL02_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2572492.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(8166..9107)
                     /locus_tag="NBL02_001811"
     CDS             complement(8166..9107)
                     /locus_tag="NBL02_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2572493.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            9191..9538
                     /locus_tag="NBL02_001812"
     CDS             9191..9538
                     /locus_tag="NBL02_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HCD2572494.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(9608..11269)
                     /locus_tag="NBL02_001813"
     CDS             complement(9608..11269)
                     /locus_tag="NBL02_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCD2572495.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
BASE COUNT         3288 a         2340 c         2100 g         3864 t
ORIGIN      
        1 atatagatgt aataaagtca ttgcattcaa actttatgaa tgtaaggaca ataaaaaaga
       61 gtaccagatg aatcaattaa aatctgcccg accctttatc attatgcttt ttttctgttt
      121 ttacgattcc tattagtatt attctaaatt aacaaaaatc agcgaagctc gtacttcttt
      181 ttctaggatt tattttggat agttctctgt ttctgtatta tatgattggc ttcaagtaat
      241 aagaaggaat ttacgaaaat tgcttctcaa atttcggaaa aagggggtat gacacaacta
      301 gtgtcatacc ccctttgaat ttctatgcga tttctgagcc atttggcatg gattttggtg
      361 cttcgattac ttgtaaagag ccatctggtg cttctgcaga aaggatcatt ccttgactga
      421 tttgtccacg catctttcgt ggtttcagat ttgctacaat gacgactttt ttcccgatca
      481 attcgcttgg atcaggataa aattcagcga ttcctgacaa gatttgacgg tcttggctgt
      541 ctccagcatc tagtcggaat tgcagcaact tatctgcccc ttctactttt tgacagttga
      601 tgacttctgc tacttttagt tctacttttt caaatacatc aaatttgatt tgtttttctt
      661 tcgttgaaac aagttctgtt tcttctggat cccatttgac tgtatcttcg tttgtttgag
      721 taccttcaga cattttttct tgaataaaag caacttcttc ttccatatct aaacgtggga
      781 agattggtgt accttttgca actacttttg ttcctgaagg aaattcgcca aagtgtaatc
      841 cttcaagatt cattgtttca gagtccaatc ctagttgatt gaagatcttc tctggcgttt
      901 ctgtcatgat tggctgaagt aatatcgctg cgatacgcaa agattcagct aaatggatca
      961 tgacactatt caattcagct ttcttttctt cgtcttttgc taatacccat ggttctgttt
     1021 catcaatgta tttgtttgca cgagaaatca gcacccagat ttcagccaat gctgtattga
     1081 attccatttt ttccattgca tcatggtaac gtccgacaac gttagcagcc gtcgtagaca
     1141 gttcactatc aaatggagtg acaagtgagg catagtcagg aacgagtcca tcacaatatt
     1201 tgttgatcat agcaatcgtt cgattcaata agttccctag atcatttgcc agatcataat
     1261 tgacacggga aacaaaatct tctggcgtaa agacaccatc tgacccaaac ggaacagcac
     1321 gaagcaaata ataacggagt gcatctaaac cataacgttc cacaagcatt tcaggataaa
     1381 cgacatttcc ttttgattta gacatttttc cgtctttcat caatagccag ccatgcccaa
     1441 atactttttt aggtagaggc aaatcaaggg ccatcagcat gattggccaa tagatcgtat
     1501 gaaagcgaac gatttcttta ccgaccatct gaacatctgc aggccagtat ttttcgaata
     1561 gactttcatc ttcggagcca tatcctagtg cagtaatata attggataat gcatcgatcc
     1621 atacatacac cacatgtttc ggatcactct tgactggtac tccccaagta aaagttgttc
     1681 gtgaaactgc aagatcctct aatcctggtt tgataaagtt gttgatcatt tcgtttttac
     1741 gagattctgg ctggatgaat tcgggatgtt cttcatagta ttccaataga cggtctgcgt
     1801 atttgctcat gcggaagaag tatgattctt cttttaccaa ttctacttcg tgtccacttg
     1861 gcgctttacc accgatcacg ttaccttcgt catctttgta cacttctgca agttgtgttt
     1921 ctgtaaagaa ttcttcgtca gaaacagagt accagccttc atattcacct aagtaaatat
     1981 ctccctgttc taataaacga tcaaatattt tttgaacagc tgctttatgg taatcatctg
     2041 tcgtgcgaat gaatttatca taactgatat caagcgtttt ccataatttc ttgacatcag
     2101 ccgccatttt atctacatat tcttgcggtt ttacacctag ttcttctgct tttgtttcga
     2161 ttttttgacc atgttcatcc actccagtga gataaaacac atcaaagccc atcaaccgtt
     2221 tataacgagc aattgcgtca catgcaatcg tagtatacga gttgccgata tgaagtttgc
     2281 cgcttggata atagattggc gtagtgatat aaaaagtttc cttttctgcc acggggctct
     2341 tcctccttta tcgcatgatg cgaattcgtt tccttgctag tataccataa aaaaagcatg
     2401 ctgttagctt tttttcaaaa tgaaaaagac tggataaacg acttctatct acgttcaatc
     2461 tatcctttaa aaggatgaat aggttgaata aatgcgggtt tttgttttgt ttcatatcta
     2521 ctaaaaaacg gctttttatt tctcccctca cacgtggtat aataaagact atctatctta
     2581 gtaagggact ggaacaatca tgcagaagaa acatcttttt tttacactta gtattgcttt
     2641 tctttcatta gcacatttaa tattcagcta tttttatatt cgtatgtatg gttattttaa
     2701 cctccatggg catttgaaca gttttatgac agcagcttgg attttgcgtt ttatcattga
     2761 cgtatatatc gttatttgtg gcttctttgc cattcgtgaa gagcggtata aagtattgcc
     2821 gttttatctg cttttctttt tattcaatct catacttcca tttatttttc atatttaaat
     2881 gataacctag atttttcaag taaatccttg gttttttttc agagtctgct acaataaggt
     2941 aaaaagtact tttactgtca atggaggtgg tcagatgaac cgttttgctt ctaaagaaga
     3001 agaaaaatta tattatgaac ataaagcaag tgaagaagaa tttcttaact ggtatcaaaa
     3061 acaggaacgt ccagaatatg acaagccctc tttgactgtc gatatcgtac tgatgtgcta
     3121 taacaaagaa gaagatcagc tgaaagtttt gttgatccaa agaaaagggc atccttatcg
     3181 aaattcatgg gcattgcctg gtggctttgt ccaaaaggat gagtcaactg gagaaagcgt
     3241 cttaagagaa acaaaggaag aaactggtgt ggttatttca aaagaaaata ttgagcaact
     3301 gcatacattc agtacgccta atcgagatcc gcgaggctgg gtcgtcacag tcagctactt
     3361 agcctttatt ggagaagaac cgctaatcgc cggagatgat gccaaggaag tacgctggtt
     3421 cacactagaa cgtcatagta acaagatcca tttatctagc ggagaagtgg ctatctcgct
     3481 tgatttagtc actggagagt cttctggcaa agatactttg gcttttgacc atagtcaaat
     3541 catcttgaaa gcattcaacc gtgtagtcaa taaaatggaa cacgagccgc aagttctaca
     3601 agttttaggt aaagatttca cgattacgga agctcgaaaa gtatttgcta aattcttagg
     3661 gatcgattat aaaacgatcg atcattccaa ttttaagaaa gctatgctcc aatatttcga
     3721 tgagatcggt gaacgtcctg taggaatcgg ccgtccttca aaaatctatc agctcaagcc
     3781 ggggcaccat gaataaggtt gtgagcaaaa gattcagacc taatcattca agaaaaaacg
     3841 caaaaacaac tctttgtgag ttacgttttt tgtttcagaa ttttgatgta ttcgtaaact
     3901 ttctgaataa aaaattaaca atagcagaaa aagctcgtac gaactttttc tgctattgtt
     3961 ttattggttt gtcataatta gtgagctttt cttcaaaaga aaaattccta gaaacaaaag
     4021 tcattaagac tttttattaa caaggctaac cctaacgctc ctatcagttt cattcatttt
     4081 ttatcccttg ataaccgaat gtctgcccat tttttctact acttggctct ttcttacgga
     4141 attgctttct tctgctccgt cggtattgat caaaagaaat tttttattat accattggga
     4201 cttataacgc aattttgtta ttaatttatg ccagtcgtga atagaaaaat cagcatgtgc
     4261 ctcttctttt ggaaaagatt ctacaaataa gtagtcatat tgtttgacca atttcccaga
     4321 taattgttca agataatctt ctcgctggtt cttttgtttc agataaagat ccattacttt
     4381 tctcttttgt ttctgataat tgcttgcctg atctaacctg atctttctat ggtgagcgga
     4441 gcggactttc acattcaatt tccgctgtgc atgttgcagc ttttcttctg tctggtgaaa
     4501 ttttggtaaa gttacttcta gaggtcgaga tgtttggatc aatgtcttcg gatgataggc
     4561 gatcccgatc catttagcta aagattcttt ttttactggc gaaacatcac aaagtagtgc
     4621 gacataaaat tcttcattat atcgagcaga aatcgtagcg gagcgaatcg atccttcaac
     4681 tggccgatgc tgatttatct tgattagttc tttttgtttt ggtaacttta agtagccatt
     4741 ttccagatag atcgtacggg tttggttgtt ggtcgtataa gattgccaca tacttttttt
     4801 cgttttaagc tttgggaagc tggcacgtcc cttgaaataa ttgcgaaaag cccgatccaa
     4861 attaagctgt gcatttgcta aagcaagact gtctacttct tttaagaatg ggtattcttt
     4921 ttttaatgta gcgggtgtga gcgaaaaatt ggctactcct gtttgttgcc aacttttctg
     4981 ccgctttttc agcaaatggt tataggtaaa tcgagcacat ccaaaagtac gaatcaattg
     5041 ctgtttttgt tcttctgtcg gatacatacg gaatttaaaa gcttttagca tctatgtccc
     5101 tcctagttag caaccagaag agattccttt tgtcgtttta gtttttttgc gtaatatggg
     5161 acttcctgct cagttgcata gacaaaatgt tctggtgcga tttcacgaag ttgacgtact
     5221 tgtccgtcat ctggttcagg ttgatatttg attcttctgc gttttctctc atggtctgga
     5281 tctggtagag gaatagcaga taacaaactt tctgtataag gatgaacacc gtgattatag
     5341 atttcatcac ttgttcccat ctctaataac aagccgttat gcattacacc gattcggtca
     5401 ctaatatgtt taaccatcga caaatcatgt gcaataaata gataggtcaa ttgatgttct
     5461 ttttggagat cttgcaacaa attgaccact tgggcttgga tcgacacatc tagtgcagaa
     5521 attggttcat cacaaatgat aaaacgcggt tttactgcca atgctcgtgc aataccaata
     5581 cgctggcgtt gcccacctga aaattcatga ggataacgag taccgtggct tggattcaat
     5641 ccaacagttc tcaatagatc atcgactttt tttgcacgct cttcagatga tttagctagt
     5701 ccattaacat caattccttc ggcaatgata tctctgactt tcatacgagg attcaacgaa
     5761 gcgtatggat cttgaaagat catctgaaca tcatgccgga atttcgtcaa acctttttta
     5821 tctttgatct tcgttatatc ttgaccgtcg aataatatct caccatcagt aggttcattc
     5881 aagcgaatga tcgtacggcc agtagtggat tttccgcttc cagattctcc aactaaaccg
     5941 aatgtttctc cttcataaat gtgaaagctg atgtcattta cagcatgtac ttcattcttt
     6001 tttcctacat tgaaatactg tttcaatcct ttaacttcaa gtagtttatt tggcatcagc
     6061 aattcctcct tgaccgtaag tatgtttttg tttctcttta aagatagccc agcgacgttg
     6121 gatttcttct ggcggtgtca ctttgggtgc ctgcggagca agtagccaag ttgctgcctt
     6181 gtgtgttttt gatagttcaa aaaatggtgg ttcttgttct gcatcgattt tcatcgcgaa
     6241 ttcatttctt ggatagaatg cgtctccttt tggcgggttc aataaatccg gtggagaacc
     6301 aggaatagca tacaatcgat cgttcgcact atctaatgtc ggcattgaac caagcagtcc
     6361 ccaagtatag ggatgttgcg ggttgtagaa aatttcttcc gatgttccaa cttcaacgat
     6421 tcttcctcca tacatgacag cgacacgatc agcgacattc gctactacac caaggtcatg
     6481 agtgataaag ataattgatg tatcgatttt tgtctgaatt tctttcagca gctccaaaat
     6541 ctgtgcttga atagtcacat caagcgctgt tgtaggttca tcagcaatca ggatttccgg
     6601 ataacatacc agtgcaatcg cgatcacgat ccgttgacgc tgacctcctg agaactgatg
     6661 cggataactg ttgatacgtt tctcagcttc gggtattcca actaatttaa gcaattctaa
     6721 tgcagctttt tgtccctctt tttttgaaac tttattatgc tttctaaggg actcagcgac
     6781 ttgcttccca atgggcatcg tcggatctaa agaggtcatt ggatcttgga aaatcatcgc
     6841 tatttcttta ccacggattt tttgcatctc tctttctgat tttccaacga tatcgttacc
     6901 tttaaagagg atttgaccag catctatatt ggcgttgctt gacagcaagc gcatgatgct
     6961 tctggtcgtc actgattttc cacttccaga ttccccaacg atcgccaatg tttctccttt
     7021 attcaaatga aaattgacac cgcgaatcgc tcgtaccttt ccagcatacg tatcaaaaga
     7081 gatttctaag tctttcactt ctaaaatttt cgtcatcgtc ttcactcttt cattttagga
     7141 tcaaaggcat cacgcaatcc atcagcaagt aagttaaagc caatcatgat aaccgataaa
     7201 gttactgccg gaatccataa tagataaggc agatacagga atgtcttgta cccttcgctc
     7261 aacatcgttc ctaatgaagc tgacggaggc gtaagaccta aaccgataaa gcttaaaaag
     7321 gcttcgaaga aaatagcaga tggaatacta aacatcattt gaataataat gacactagag
     7381 atattcggca aaatatgttt gaatgcaatt ttcattcttg attcacctaa tgtcaatcca
     7441 gctaaaacat attcttgatc ttttaatttc attgtttgtg cacgaacgat acgtgccatt
     7501 ggtatccagt ttgtgatggc catagcagcc acgattgaaa aaattcctgg ttcaaaaacg
     7561 accaacatca aaatcatgac aacgaggttt ggaattcctg ataagatttc caaaaaacgt
     7621 tgcatcacgt tatcgacacg tccgcctaac aaaccagaaa ccaacccgta agctacacca
     7681 atcgttacat ctagtaatgc agcaataaat gcgatcagca acgagattct cattcccata
     7741 aataagcggc ttaatacatc acgtcccaaa ccgtctgttc ctaaaaagaa atttacgttt
     7801 gaagggacgt ttgcttgagc atatttatca acaagttcac caccaaccat tgcttttcca
     7861 ttcaatccat cgatattgat tcccggaata cgaggaggca aattgatata agccacgttt
     7921 tgggcagttg gatcatgagg agaaacaaag attgtgatca cagagatcaa aatgatgacc
     7981 aatagtaaaa ccattgaaat aacggcagct ttgtttttct ttaaacggcg ccatgaatct
     8041 tggataaaat tcaaagaagg tgcagtaatc tgttcacgtt cttcttcggt atttttttgt
     8101 aatggaagaa attcatccgc agggatatct gcaactgttt cataacgctt tggtatcatt
     8161 tccatttatc ctcggctccc ttctgaaaca cgaattcttg gatcaactag accgtacaat
     8221 agatccacga tcaaaataac aaaaatcagc ataaatgagt aaaggatcgt tacagccatg
     8281 atcgtaggat aatcatttgt tgtgattgac ttaacgaatt gttccccgat accaggaata
     8341 gcaaagatat tttctaccac cagtgatccg gtcatcaatg ctacagctaa cggtcctaat
     8401 aaagtcatca atggaatcaa actatttcga agaccatgtt taaaggcgat ttcccaacgg
     8461 ctcaatcctt ttgcttttgc taattccaca taatcactat gcagtacttc caccatttcc
     8521 gtacggataa accgagcaga gtcagcaagc ggcgacatgg ccaacgcaat tgttggtaag
     8581 attgtgtaca caaagccatc ccattttgcg attggcaaaa cttgtaattt gattgcaaag
     8641 atatattgta ataaaacagc aaatacaaag tttggaatgg aacgacctaa tatcgctacg
     8701 agtgtagaag atgtatctgc ccatgtattt tgcttcatgg cagagatcgt tcctaaaatc
     8761 gtccctagga ctgttccaaa gataattgcc tgtaacccta actgcaagga tggtccgatt
     8821 cttccagcta acaaatgtgc gactggctgg tttttaaatt ggaaggaaat tccaaaatct
     8881 ccttgaagca aattagaaag ataaatccca tattgtacga taactggttt atccaaaccg
     8941 acttgtttat ttagcatctc gattgtttca ggactcaaac gttcctgatt cgtatatggc
     9001 gtgcccggca gcaattgcat caagaaaaag gtgattgttg caattaacca aagcgtgatg
     9061 atcatgaaaa agacacgttt taatacatat ttgatataac tattcactaa tgtttccccc
     9121 tagcttttga cttctgacag atgtttcgct acattattag ataatgaacc aaaaataaag
     9181 gagcaatcaa atgaaaaaaa aatctatccc ctacgcagta gcttttctac tgattctggt
     9241 tattttaata aaaaatctca tcaaccattc ctttacactc attcaattat ctaatgatct
     9301 attcttatgg tcattgccgt ttctgattat cggcggtttt ttatgggtct tttcttcggg
     9361 tttcttcgat cattttcagc gatcggtcca tcttgcacga acaaggaacc gaaagaaaaa
     9421 accagaattt tcctcgctct cgtctgccag ctacggtatg tacagctttt ggctgatcat
     9481 tgccggaata ctaattgctc tttctgctat tttcatgctt ttttctttgt taggataagt
     9541 gaatatgaga acgaggttgc gacatttgcc gcagcctcgt tttttctcat gctcacggta
     9601 ttaacgatta ttcttcaacg tatgtccatt tgtaatccca tgaagcgcct gccgcatggt
     9661 gaacgatacc tttgattttt tcattcacta ggtgaccttc tgctttttgg tacaccggaa
     9721 cgacacccat atcatcagag atgatcttga ttgctttttg gaaatcatcc caacgggctt
     9781 gttcatcgcc agcgtctgtt gatgaagctg ctttaactgc ttcgtcatat tctgcattgc
     9841 tccagcgacc acggttgtat gaattacctg tgacaaacaa atcagtaaag ctgcttgggt
     9901 ctgcatagtc agcagcccat ccgcctagta ctgcgtcaaa atcacccgaa gttgagcggt
     9961 caagacgtac agagaatgga acaggtgttg gtttgactgt tacgccatcc aagttttctt
    10021 ggattgagtt ttgaacatat tcgattactt ttttcgatga atcatcatca gaagccatca
    10081 aatcaaattc taaagaatca acgcccaatt cttttttcgc tttttcccaa tattcttttg
    10141 ctttatcttt gtcataagaa actaattttc cagcttcttt tgcaaagtct tcgttcgttg
    10201 ttggactttt taccatatct gatgggatca atcctgtaga gacaactgat ccgtcaccta
    10261 gtacttgttt tactaaagct tcacgatcga tagaataaga aatagctttt cttaggttca
    10321 cattgttgaa tggtgaattc ttttcacgtt gattgaattc gatatagctt gtccgcgctt
    10381 ctttaacaga agtataagct ggatcgtttg cgttttgctg tgctaactca ccagacaaga
    10441 tcacgtcatc tgcttggcca tctttgaata agttcaatgc agtagaagat tcttttacta
    10501 cactgacttt gatttcagaa agttttactg tatctttgtc ccagtaatga tcatttttct
    10561 tataagtcca ttctgtatct gttcctggtc catcaaaatc ttctaatgtg aatggtccat
    10621 tataaacagc tttatcactt gcagatgcat aggcatcccc attttcttcc actacggatt
    10681 gtttttgtgg gaaaaatgat gggaaagcaa gcagatattg gaaatatggt gtttgtttcg
    10741 tcaatttgat ttctaattca tagtcaccaa ctgctttgat ccctagttct gatttatctt
    10801 tctttccagc tgtaatgtct tctgcatttt caactggcgc atacaggtaa gcatattcag
    10861 aagctgtagc tgggtctact gttcgttgcc agccataaac gtaatccgct gctgtaacag
    10921 gatcaccatt tgaccatttt gcgtcttctc gtaatttgat tttgtaagtt aatccgtctt
    10981 cactgacttc tgccaactcg ctagctcctg ccggctgtgg tttgctgtct tcatcaagac
    11041 ggtaaattcc ttcataaacg ttattcaatg ctgtaaaact gattgtatct gttgcaactg
    11101 ataagtcagc tgtaggcatt tcttgaggca cgactaagtt aaatacgcct cctccatctg
    11161 ctgaaccgct ttttgctgaa ctactgcttg tggctgaact gccgccataa catccagcaa
    11221 gtacaaggcc gcataccgca accaaaccaa atgtaaattt ctttttcatt ttacgtaccc
    11281 ctttattgga attttcagaa taattaatat tttctgaagt tgtttaagag tttaatgaat
    11341 aattattaaa aaatcaacac ttttttttgt ctttttcata aaataatgat tttttcgtga
    11401 ggtttcaagg aatttttcag aaaaaatagc tttgtacaag gttttccatg gtcttccaaa
    11461 agccattaaa taattttcgt tatattgtct attattgcca tacaaagata aaaaagaata
    11521 aacaaaaaga aactgtgaca aaagtcttgt cacagtccct tatctgaata aacggtgttc
    11581 aaaagccagt tt
//
LOCUS       DAHHZJ010000089        11591 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.090, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000089 DAHHZJ010000000
VERSION     DAHHZJ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.090"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            165..959
                     /locus_tag="NBL02_001814"
     CDS             165..959
                     /locus_tag="NBL02_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2572496.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            949..2991
                     /locus_tag="NBL02_001815"
     CDS             949..2991
                     /locus_tag="NBL02_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2572497.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            3272..3421
                     /locus_tag="NBL02_001816"
     CDS             3272..3421
                     /locus_tag="NBL02_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HCD2572498.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            3432..4946
                     /gene="dltA"
                     /locus_tag="NBL02_001817"
     CDS             3432..4946
                     /gene="dltA"
                     /locus_tag="NBL02_001817"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HCD2572499.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            4943..6148
                     /gene="dltB"
                     /locus_tag="NBL02_001818"
     CDS             4943..6148
                     /gene="dltB"
                     /locus_tag="NBL02_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HCD2572500.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            6193..6426
                     /gene="dltC"
                     /locus_tag="NBL02_001819"
     CDS             6193..6426
                     /gene="dltC"
                     /locus_tag="NBL02_001819"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HCD2572501.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            6429..7697
                     /gene="dltD"
                     /locus_tag="NBL02_001820"
     CDS             6429..7697
                     /gene="dltD"
                     /locus_tag="NBL02_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HCD2572502.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            7838..8920
                     /locus_tag="NBL02_001821"
     CDS             7838..8920
                     /locus_tag="NBL02_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCD2572503.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            8920..9723
                     /locus_tag="NBL02_001822"
     CDS             8920..9723
                     /locus_tag="NBL02_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HCD2572504.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            9793..10533
                     /locus_tag="NBL02_001823"
     CDS             9793..10533
                     /locus_tag="NBL02_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCD2572505.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            10544..11548
                     /locus_tag="NBL02_001824"
     CDS             10544..11548
                     /locus_tag="NBL02_001824"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HCD2572506.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
BASE COUNT         3732 a         1890 c         2368 g         3601 t
ORIGIN      
        1 attgtgaaaa aaggtgaaaa cttgtagctg gtggatttgt tttttgcata tttaattttg
       61 ctgaataaaa taattgaata aaatttttta ttcagtccaa gtaaaagtga caaaaacata
      121 agagcaaatt attcgtagat atgctactat gcaaataaat agaaatgaaa aatgaaagag
      181 gaggagaaga attggaaaag atgatcgaag tgaaacatct aagtaaaagc tacggcagca
      241 aactttctcc cgtcaaagcc ttagataata tctcattcac agtagaagaa ggcgagtttg
      301 ttgggattat gggcccaagt ggtgcaggaa agacaacgct gatgaatatc ttgtccacga
      361 tcaatttgcc tacgatgggc agtgtctcga tccaaggaaa agaaatcaca aaaatgaaaa
      421 acagtgagct cagtgacttt cgtcgaaaaa aactaggatt catttttcaa gagttcaatt
      481 tgatcgatac cctaaatgca aaagacaata tcctattgcc cctagcagtt gaacggatga
      541 ctaaagagga aatggaaaag agagtaagac atgtagctca attgttgaat atagaagaac
      601 tgctcaaacg gtatcccgat gaactttctg tcggacagag acaacgaatt gcagctgcta
      661 gagcacttgt tgttcagcca caagtgattt ttgcagatga accaactggc tctttggact
      721 cgaagtcggc tacagaattg ttgaactact tgaagaccat gaatcaaaaa gaaaaagcaa
      781 cgatactgtt agtcacccat gatccatata cagcaagcta ctgcgataga attttattta
      841 ttaaagatgg tgtgattttt tctgaagttg tcagaagagg gacaagaaga gagttctttg
      901 agaaagtaat agacatgcag gcgacgattg gcggaggagg aaaaatgaat gctgtataaa
      961 ctagccgtta aaagtttttt tcggcagtca cgaggctatc ttgtttattt tttcagtttg
     1021 actttatccg taatgatcta ttattccttc agcgcaatga cctacgatca atctttagtc
     1081 agaagagcaa gtcaagatac ttcgatagat ggtggattga gttttggtgg atttatcatt
     1141 actgttgttt tgcttttttt cgtattcagt gccaaccgat ttttcttgaa tagaaggcaa
     1201 aaggaaatag gcatctatca gctttttggt atgaataaac tgcagatatc aggtatatat
     1261 gtgttagaaa tcatgataat tggattgttc gcctgtattt ctgggatatt actcggtatt
     1321 attttttcca aactcttttc aatgattttg gttcgaatga tggatatgga tcttactagt
     1381 ccatttttta tctccattcc atcagtagta gatactttat ttgctttttt catcatcctt
     1441 ttagcagtat ctgtatattc aatatggaaa atatggcgct atccaatgat tcgaagtttt
     1501 ggagaaaaag aacaagcaga tagttcaaca atgcgcttca ggacaaggca ccgattactc
     1561 gcagtcatcg ggttactact tttaacgagc ggatattttg gagctagtca ttttagggaa
     1621 atcacttcat ggatgatcag ttatcattat cttgatacat tagttttctt ttttcctttt
     1681 ttaatttttt tcgtttgtgt ggtcggtact tatttgtttt tctgttatac atttcggtta
     1741 tttttgatca tattgagtaa atggaagatt tattactatg gtatcaattt tttgataatg
     1801 ggtaacacgc aagttcattt gctcaaaagt tggcgtacaa attctctgat tacagtcatt
     1861 ctcggaattt ctttaatgac aattggcggc acggctagta tctctactat tttatcctac
     1921 caggagaaag tttcctcccc gatggactat cagtttgatg ttgagacaga gaaaaaaatc
     1981 aagccgattc tagcagagga agggcagaag ataactaaag aaatcacctt gcattataaa
     2041 gtcattggga gtatctatag tcaaaatctc gaagaaccta tgtttcaaac tgctaatctg
     2101 atcacggaaa cacaatatca agccttcaga aaagttaatc cagatttgcc gaacatttct
     2161 ttgaaatcac ctggtcatac tgttttgctt gatcaaatgc aaactctttt tcggaatatt
     2221 tcattatatg gatctagtat tggactgcca gataatcaag aattgaagat tcaacaaatg
     2281 cttccaagtg tattaggtga cgaaacaatg gtatatctaa gtcctgtact gattgtagat
     2341 gataccgttt ttgaaaaagc aaaaggtgtg gattatcaag tagtcaatgt agatgtatca
     2401 ggtggaaata atgaaaaaat agatgaacga ctgagcaatg acctgtcgat aaagtggctc
     2461 aatgctgttt attattctta tgatatcgta aatggaagtc tacaaggaga aattagcaca
     2521 aagaaacttc cggatgacca aatgaaggaa gcacaattta gccaagaaag ctctcgtttg
     2581 aatttctctt ccagctattc gaaattgcgt gcagttaatc ggcgaatcgg tatcaatatt
     2641 tttgtcacac tatttgtcgg gatgatcgtg attattgcta ctggtagtat tttgacagtc
     2701 cggcaattag cagaagcaga ggaagaaaaa gaaaattatc acctattgac taagctgggg
     2761 attccacaaa gaaggatcag acggatgatc ttcgagcaaa acgcacttac cttctttcca
     2821 cccatgatat tagggatcac tcacgcagta tttgcaatca atgtcttcac tcagtatata
     2881 aaaactgccg attactggtt ggcttatctt gtgagtggct tattgatcct tatctatctt
     2941 ttgctttatg tcattacgag tcagttatat tgtcggatta tcgaggaatg atatacggaa
     3001 taagacaaga taataaatag aactaaattt tgaataccat ttatttggaa taagaggttg
     3061 tgacacgatt tttgtcacaa cctctttatt ttttatattt tttaaaagta cgagaaatta
     3121 taaagaaaaa agcagtgatt tgatgtgcta tttttataaa agctatgtga caaaactgtt
     3181 agtaaaaaaa cggtttctgt aagctagaca catgtttctt tttgtcaaaa ttagttacac
     3241 tttgtgtgag gttatatgag gagggaatcc aatgaacaaa tggcgttcta atccaaaatt
     3301 gaattattgg ggaaaattca tcattcgaac aattttttat agtgcagtac tgctgttcct
     3361 gatttatctg tatcactata aaaatattca aggaggaact tttatctata acgaatttta
     3421 ggaggaagag tatggaaatc aaaacgatta ttgaagcaat cgatgattgg ggaataaatg
     3481 aacctgatcg agttgcttat caggcggatg agacgcatac ttttggtgaa ttgaaacaag
     3541 catcagacgc acttgcttat tatttagaaa aaaaggtaga aggcgatgga ccaattgtcg
     3601 tttttggcaa tttggagttt gaaatgattg tctcatttct aggggttgtc aaatctggcc
     3661 atgcctattt gccgattgaa gagcatacac ccaaggagcg tatcctatcg atctttcggg
     3721 tggctaaacc atctatggtc atcagtatcg gggactggat agaagatatt ccttcggttc
     3781 ctgtcataac aaaaaaagaa tttcaagaaa tcaagaagat accagtagga ttttatccca
     3841 gaaatagcgt aaaaggtaca gaaaattttt atattatttt tacttctggt actactggag
     3901 aaccaaaagg cgtacaaatc agtcatgata atctagtcag ctttgttcaa tggacgatgg
     3961 aagactttgg gatcaagcct ggtatgcatt tcttagccca agcgccattt tcttttgatt
     4021 tatcggtatt ttctatttat cctgcattag tttctggcgg aatgctgaag ccgttaaata
     4081 aagcagttgt tcaagacttt cgtcaattat tcgcgacatt gccagccttg aagttgaatg
     4141 tgtgggtttc tactccatca tttatggata tttgcttgat ggaacctaca ttcaatggag
     4201 aaaatgtgcc ggaattggac atgtttttat tttgcggaga agaattgacg aaaaaaactg
     4261 cagaaagtct acttgatcga ttcccaaatg cacggatata taatacttac ggtccaacag
     4321 aagcaactgt agctatttca agtattcaga ttgaccagca tgtactagat agttatgacc
     4381 gactaccgat tggctacgta aaaaaagaca cgaaagtttc tattgtccaa gaaggtaaac
     4441 ctgtttcaaa aggagaaacg ggtgaaatca ttattgcagg accaagcgta tcaaaaggtt
     4501 acctgaacaa tcccgaaaag acagctgctg cgttcttttg tcaaaatgac ttagcaagtt
     4561 accatacagg ggatgcaggt agactagatg aatatgggct tttattttat gaaggacgta
     4621 tggatcagca ggtcaaatta catggatacc ggattgaact cggggatatc gaacattatc
     4681 tgctccaaga caaccgaatc aaacaagctg tcgtcgtacc aaaataccaa ggaacaaaag
     4741 tacagcagct ggttgctttt gtcgtattag aaaacaaaac gaaacagccg gattttcagt
     4801 tgactaaatc tatcaaagaa caattactag aagttgtaat ggattatatg attcctcaga
     4861 agttccatta tgtcgatctg ctgcctcaga cagttaatgg aaaaatcgat cgaaaaaaat
     4921 taacagctga ggtgaatcct gaatgatgtt acctcatatg atcccttatg cagagccgtt
     4981 ttattttttt cttttagcca ttgcgcttgt accgatcgtt ctatctttat tgatatggga
     5041 aaaaagatta ccagtttatc aatcagcagt gacacttttc tttttatttg tcagctttgg
     5101 aggtaattat tggcaacagg gagcggcgct gattgggtat gttttgtggc agacgatact
     5161 tgtatggggc tattttgttt atcggcaaag gaaaaatcag acaggatggt tttttgccgc
     5221 cgttatttta gcgataattc cactgttttt aacgaaagtg actccttttt ttgaacatgg
     5281 aaaagcatcg ttacttggtt ttttaggaat ctcttattta acctttaaat ctgtacaaat
     5341 gatcatggaa ataagagatg gcatgatcaa aacatataag ccatttcgtt atatccagtt
     5401 tctgctgttc tttccaacaa tctcttctgg accaatcgat cgctatagga gatttgaaaa
     5461 agatcagctc catccgcctg ttcgtgataa atatgttgat cttttgggaa aaggcgtcca
     5521 aaatattttt gtcggttttt tatataaatt cattatcggt tattattttg gtcaagtact
     5581 gcttcctgta gtgggacgca ttgccttaca gcatggtggt atttcatggg cattagtagg
     5641 ttatatgtac gtgtacagta tgtatttgtt ttttgatttt gctggctata gcttatttgc
     5701 agtcggtaca agctacatga tgggatatca gacaccaatc aatttcaata aacctttttt
     5761 atcatggaat atcaaagaat tctggaatcg ttggcatatg acgctgtcct tttggttccg
     5821 tgattatgta tatatgcgat tgatgttctt catgatgaag aaaaaatttt ttaaaagcaa
     5881 gatcgtaatg tcaaatatcg gttattttgc tttgtttctt ctaatgggta tatggcatgg
     5941 attgacgtgg tactacatcg tatatggact gtatcatgct tgtctgatct gtctgacaga
     6001 tgcatggatc cgatttaaga agaagcataa agagcagttg ccttctaaca aatttacgca
     6061 tggcttagcc gtatttttga catttcaagc agtatgtttt agcttcctga ttttttcagg
     6121 atttttagat aaactttttt ttagctaacg aatagtgaca tattaattta tataacaaag
     6181 gagaaaagga aaatggaaga acaaatttta tcaatcttag aagaaataac aggaacagat
     6241 gaagtaagaa aaaatcgtga tattgatcta tttgatgaag ggttgcttga ttctttagca
     6301 tctgttcaat tattagtcga gctagacgga gagctggata tccaagtccc tgtatctgaa
     6361 ttcgagcgag aggattggag cacaccgaat aaaatcattc aacaagcaac tgcattgaag
     6421 ggttgagtat gaaaaagaaa ctttttgcta ttttcggccc ggttgtttta gctgttggct
     6481 tactgtttgt gttttttacc tcctcctatc aagtagatat ctctaatcca gaaacgatta
     6541 gacgtgcctc ttcttccatg tcagtgaacg tgttgaaagg agatgcgata aaaaattatg
     6601 cattatctga aaaacaatat attccttttt ttggctcaag tgaattaagc agaatcagtc
     6661 catttcatcc gtctgttctg gcagaaaaat accagcgaaa ctatcgtcca tttttgctgg
     6721 gagcacccgg gacacaatcg ttaagccaat atatgatgat gcgttctgca ggagatgcta
     6781 tgaaaaacaa gaaagtcgtc tttattattt ctcctcagtg gttcgttaaa aatggtgtaa
     6841 aaactgatta ctttaatacg tattattctg aacttcaaac atacgactgg ttgttttcaa
     6901 tgaagaaagt aacgcctgct gatcgctatt tagctagaag attattaact ttctcaaaag
     6961 taaaagagaa tgatacgttg acggcgattc tgcaaacgat caaaaaaggg aagctacctt
     7021 tacctgaatc tttgaatcag ctgcgcagcc aatggaacat gctgaagaga gaggatgaag
     7081 tattcagcaa tattgggttg aaagatcgac agcaaaaaat cgatcatgag tcaaaacgat
     7141 taccaaaaca ataccaagaa acagaattat ccattttagc aaatcaaatt ggagaacgag
     7201 aaacgacgaa taatccattc gggttgaaaa acgattttta tacgcatcgt atccgagccc
     7261 acgagccaga gctcaaacag tcacaaaaaa attgggatta tcgtttttcg ccagaatttt
     7321 ctgattttca gctggtcttg gatcaattag caaaaaacca taacgaagta ttgttcatca
     7381 tcccaccggt taacgagaaa tggagcgatt ataccggact atctcaagaa atgttacaag
     7441 gatttgccaa aaagatcaaa tttcaattaa atagccaagg attcaatcga atagctgatt
     7501 ttgtcaatca agcgggtacc aactatttta tggaagacac gatccatcta ggctggaaag
     7561 gctggttggc tgcagatcaa cagatcagac cgtttttaga agaaaatcat attactgcta
     7621 gtaagtatca tcttgacgat gcttttttta gcaaatcttg gcaacatcaa ataccagata
     7681 aactacaatt aaaataaaga aaacaggcga gtgttgctcg cttgttttct ttatttttga
     7741 atattctgaa taaaagataa aaataccaag tttgcctgaa caaaaatcat aagcaatggt
     7801 ataatcacaa tatgaatata gaaatggggg aaaacatttg gatacaaaag aagaacagct
     7861 acttattgat ttaacttctg caaaaggagt acctggaaat gaagaagagg taagagaagt
     7921 attcagagaa tatgccaaac cattcgctga tgatattttt tatgacggtt taggcagtgt
     7981 gattgccaaa catggtgctg gcggaggccc caaggtcttt atttctggac atatggacga
     8041 agttggtttt atggtaacaa aaataaccga aaaaggattc ttggaattcc aaactcttgg
     8101 aggttggtgg gggcaagtca tgcttgctca gcaagttgaa atcaaaacac gtgaagggaa
     8161 aatcgtccac ggtgtgattg gttccaaacc accacatgtt ttaacacctc aagtaagaaa
     8221 taaaccttat gaaataaagg atatgttcat tgatattggc gcatcaagcc aagaagaagc
     8281 caaagagtgg ggcatacgtc ctggagatat ggtcactcca tatattgaat acaaacgaat
     8341 gaatggcagt aaatatttac tggcaaaagc atgggataat cgtatcggga cagctgtttc
     8401 tctgcgagta ttagagaatc tatctaaaga agcacaccca aatgttttat tcgcaggaag
     8461 cgatgtgcaa gaagaagtag gcttgcgagg tgctcgaaca agtacacacc ttgtaaatcc
     8521 cgatattgct tttgctttag atactggtac agcaggagac actccaggaa tgacaccaaa
     8581 agaagcagat tcgattttag gaaaaggccc acagattttg atttttgatg catctatgat
     8641 cccgcataaa aaattattga attttgttat tgatgtagca gaggaaatgg atattccatt
     8701 ccaatataca gtgatttctg gcggaggaac tgatgcagga caaatgcatt tgacaagaga
     8761 tggtgttccg tctgtagcca ttaccgttcc tgttcgttat ctgcattccc atacatcagt
     8821 gattcatgaa gatgattatt tgaatacagt gaaactagtc acagaattag tgaaacggct
     8881 tgatagtgaa acagttgaaa aaattcggag ttatgaataa tgaataatag aatacctatt
     8941 tctgaatatc ggaaaaaagc acgagtttct ttggaaaatc aatggggaat caatacttgg
     9001 cttgttttct tatctataat atttacgagc gtcatccaga acttttttga cggtttcagt
     9061 ccttttccgg aagaaagcat gcagcagcac ttattgagtt ttctagtcga taatttactt
     9121 ttattcgcat tcaactatgc attgttttac attgcgctag tagttgtacg gggtggcaga
     9181 ccaaaaagta accttttatt ttcagttttc caaagaaagt attttggttc gatacttggc
     9241 atcaatttga tcaattcagt catcaactgg ctactcaaca gtttaatctt tctgcccatg
     9301 tttttaatca gcggagtgag tacttatatg cagctgcttt tcagttcaaa tgcggtacct
     9361 acgggtgtca tgccagaaga ttttcttgat atcagctttg ttttatcgat gatagggatg
     9421 atttttgttt cagcactcat catgagtatt gttggcggtt tattccaatt tgctgcatgg
     9481 acaaagttcg actatcctga tttgtcggtt attcaatgtt tgaaatatgg ctggttttta
     9541 ttgaaagata gaattggtac gtatattttg ttgcagctgt cttttatcgg ctggtatttg
     9601 ctaggagcaa tcgctttgtt catcggtctt ttgtgggtca ttgtttatgt gaatgtgtca
     9661 gttgcagaat tttatgaaca agcacggatt gaaaaaggag gtccagacgc ctattttact
     9721 tagcatgtaa gcagtttatt catcaaaaaa ataacttttg tggtaggata aatctttgag
     9781 ggggaatgta cgatgaaatt aactgtttta ggatgcttag gagcgtatcc ttataaaaat
     9841 caagggacaa caggttattt gcttcaatct gacgggttca atttgttgat cgatgcaggt
     9901 agtgctacgc tgatcaaatt acaagaacac cttgatccac ttgatctaga tgctgtgatc
     9961 cttagccatt atcatcatga tcatattgct gaccttggtg tattacagta ttactggcaa
    10021 cttcatccta agagaaatcc ggagacattg ctttcaattt atggacacac aaaagattcc
    10081 attcactttg aagaattgac gttggaaaat gtctcaaaag gacatcctta ttttgaagct
    10141 gaagaattaa aaattggtcc ttttctgatt acttttatgg aaacaatcca tccagtagta
    10201 tgttacgcca tgcgtatcgt agaagaaaca acggggaaag tattcgtatt tacaggtgat
    10261 tcaggttatt tggaaagttt cgctgatttt gcgaaagatg ctgatttgtt tctagcagat
    10321 acctatcttt ttgaaggaaa tgaacatcat catgcccatt tcacttcaaa agaagctgga
    10381 gaacttgcga aatcagctaa cgtaaagaaa cttgtactta cacacctacc tcagtttggt
    10441 gatttagaac aattgaaaaa tgaagcgcaa aaggcagcag gcaaagggat tgatatagaa
    10501 cttgctgcag ttgacaaagt gtttaacatt taggaggaag aacatgattt ttgtaccaaa
    10561 tgaaaaccac gacccaagaa tcaatttagc aattgaacaa tatttgctgc aagaaatgcc
    10621 tttagatgaa cctattttgc tcttttatat caatgaacct tcaatcatca tcggcagaaa
    10681 ccaaaatacg cttgaagaaa tcaatcgaga ttatgtggaa gaaaatggca ttcatgttgt
    10741 ccgtcgctta agtggaggcg gagctgtcta ccatgatttt ggaaacctga actttagttt
    10801 tatcatgcct gatgatgggg attcattccg tgactttgcg aaagtaacac agccaatcat
    10861 ccaagcactt catgaattgg gtgttgctgg ggcggaattg aaaggccgaa atgatctagt
    10921 cattgatggc atgaaatttt ctggcaacgc gatgtatgct accaacggtc ggatgtttgc
    10981 tcatggaaca attatgttcg actctgatat caatgaagta gtcaatgcat taaaagtaaa
    11041 aaaagacaaa atcgaatcaa aaggaatcaa atcgatccgt tctcgtgtaa cgaatatcaa
    11101 accatttctt ccagaagaaa agcaaaaaat gacaacagaa gaatttcgtc aagaaatctt
    11161 gttgaaaatt ttcggcgtag agtcagtaga agaagtaaaa acgtacgaat tgactgatga
    11221 agattggaaa aaaatcaatc gaatctctga ggaatattat cgaaattggg attggaatta
    11281 cgggaagtct ccagatttca atttctctcg ccaaaaacgt ttttctatcg gttcgattga
    11341 agtccatttg aatgtctcag aaggaatgat caaagaagca aaaatctttg gcgatttctt
    11401 cggactagga gatatctctg acgtggaaaa aaaattggtt ggacagaaat atgataaagc
    11461 atctctatca aaaatagtca gccaaattga tgtgaagaaa tattttggtg cgatagaacc
    11521 agaagaattg cttaccttac tttattgaaa ataaaaaata gcggaatcca catatatgga
    11581 ttccgctatt t
//
LOCUS       DAHHZJ010000090        11574 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.091, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000090 DAHHZJ010000000
VERSION     DAHHZJ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.091"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..521
                     /locus_tag="NBL02_001825"
     CDS             <1..521
                     /locus_tag="NBL02_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCD2572507.1"
                     /translation="GESSIFSDKKLINSIRKLNDAQVLELQTLKPNDLVVIRLELNGK
                     KVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVRSIITKKENRQ
                     ILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTVMEGGEDAFTN
                     RAKRYGFKQIVE"
     gene            571..960
                     /locus_tag="NBL02_001826"
     CDS             571..960
                     /locus_tag="NBL02_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCD2572508.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            947..2449
                     /locus_tag="NBL02_001827"
                     /pseudo
     CDS             947..2449
                     /locus_tag="NBL02_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            3194..5020
                     /gene="ltrA"
                     /locus_tag="NBL02_001828"
     CDS             3194..5020
                     /gene="ltrA"
                     /locus_tag="NBL02_001828"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCD2572509.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
     gene            5154..5972
                     /locus_tag="NBL02_001829"
     CDS             5154..5972
                     /locus_tag="NBL02_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCD2572510.1"
                     /translation="MPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLENGITSI"
     gene            6065..7252
                     /locus_tag="NBL02_001830"
     CDS             6065..7252
                     /locus_tag="NBL02_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HCD2572511.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            7405..7548
                     /locus_tag="NBL02_001831"
                     /pseudo
     CDS             7405..7548
                     /locus_tag="NBL02_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            7553..9682
                     /locus_tag="NBL02_001832"
     CDS             7553..9682
                     /locus_tag="NBL02_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCD2572512.1"
                     /translation="MKKKLFRIVGIVFISIVLLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            9679..10686
                     /locus_tag="NBL02_001833"
     CDS             9679..10686
                     /locus_tag="NBL02_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCD2572513.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            10703..>11574
                     /locus_tag="NBL02_001834"
     CDS             10703..>11574
                     /locus_tag="NBL02_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCD2572514.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKL"
BASE COUNT         3847 a         1941 c         2563 g         3223 t
ORIGIN      
        1 gtggggaaag tagtattttt tcagataaaa aattaatcaa ttctataaga aaattgaatg
       61 atgcacaagt tcttgaatta caaaccctta agccaaatga tttagtagtt atccgacttg
      121 aactaaatgg aaaaaaagtt agtttatcga atgctagtgc tggtcaaaag acatctgcaa
      181 ttctttctat gattttggcg tatggaaatt caccactcat attggatcag cctgaagatg
      241 atttggatag tcaattaatt aataacttaa tcgtaagaag tatcattaca aaaaaggaaa
      301 acagacaaat tttaattgta actcataatg caaatatccc agttaatggt gattcagaat
      361 ggttgattag catgggagat tcaaaagaaa tatcggctga tcagtgcggg tcggtagatg
      421 aacccaatat caagccaaga atatgtactg tgatggaagg tggcgaggat gcttttacaa
      481 atagagcgaa acgctatggt tttaaacaaa ttgtagaata acactagagg gtgagacata
      541 ttattatgtc cgcccttttt aggaggaaaa atgaaaaaaa taaaaagcta taccagtatc
      601 tgggcagttg aaaaagtcat ttacgcaatc aatgattttc agttgccttt tcctgtgact
      661 tttaaccaaa tggcttggtt cgttttatct ttactgtttg tcatcgtatt tgcccatgtg
      721 cctccactat ctatgataga ggaggtcttt ttaaaatact taggcatacc ggtggctgtc
      781 acttggttta tgtcgcaaaa gacgtttgat ggcaagaaac cactgggatt tttacgatcc
      841 tttatcagct atcacttgcg atttaaggtg acttttgcag gaaagaaagt caaagaacag
      901 aagaaacgat gggatgaacc cagtactctt gtaaggagtg tgaactatgt atccgattaa
      961 gtatattgaa aataatctcg tctttaacca agaaggcgag tgttttgcct actatgaatt
     1021 agtgccttac aattactcct ttttgtcacc cgaacagaaa tttcaagtgc atgataactt
     1081 tcgccaacta attgcgcaaa atcgtgaagg caagatccat gcgttacaaa ttgccacaga
     1141 aagtagtatt cgcgccacac aagaacgatc aaaaaaagaa attaccggta gactcaaaga
     1201 agtagcgaaa caacgaattg atatacagac agatgcatta gtatccatga ttggcgatag
     1261 tcagattgat tatcgctttt ttattggctt taaactaatt gcgacagatg aagaagtcaa
     1321 tctaaagagt ctcaagaaat ctttcttttc tgggcttcaa gaatttgtct atggagtgaa
     1381 tcatcatttg atgggcgatt ttgtttcctt atccaatgaa gaaattcgct gctattctaa
     1441 gcttgaaaaa ttaatggaaa gcaaattggc tcgaagattt aaagtcaggc gagtcacacc
     1501 gagtgacctg acgtatttga ttgaacatat ttatggcgaa aaagggatac cgtttgaaga
     1561 atacgaattt cagctaccaa agaaaaagct aaaatcagaa acactggtga aacgttatga
     1621 cttattacgt ccaaatcgtt gtttaattga agaaaaacca aggtgcttac ggatggaaca
     1681 tgagaatcat gagtcgtacg tagcgtattt aacaattaat acaattgtgg gggagatgga
     1741 atttccttcc tcagaacttt tctattacca gcaacaacaa tttacattcc caatcgacac
     1801 aagtatgaat gtagagattg tgacgaataa aaaggcttta gctaccgttc gtaataagaa
     1861 aaaagaactg aaagatttag acaatcatgc ttaccaatct gacaatgaaa cgaattccaa
     1921 tgtcttagat gctttggatt ccgtggatga attggaaacc accttggatc agtcaaaaga
     1981 atctatgtac aaactgagtt atgtggttcg agtgagtgcc gaatcagtgg atgaactaaa
     2041 gcgacgctgt gatgaagtgc tcgattttta tgatgatacc aatgtaaaac tagtccggcc
     2101 ctttggcgat atgatggggc tacatgaaga attcttaccc ttaagtaaac gatatatgaa
     2161 cgactatatt cagtatgtta cgtctgattt tttggcgggg cttggttttg gggctactca
     2221 gatgctagga gaattagaag ggatttactt tggctacaat gtggatacag gtcgtaatgt
     2281 ttacctgaaa ccggcattgg cttcacaagg agtcaagggt tcagtaacca atgcgctggc
     2341 cgccgctttc ctgggttctc ttggtggagg taaatccttt agtaacaatc tcttagtcta
     2401 ctatgctgtt ttgttcggtg gtcaagctgt gattgtcgat ccaaaagggg gtgcgaaacg
     2461 ttcctacttg aaaagagtag gcacagcact tcattaaatc aatatttaat gtgtgttgga
     2521 gaactaaaca cacgaatagt cgaatgacgg ggtaacgccc tgaaaggcta ctttaatact
     2581 ctgactggca gaaacttgag taaacaaggc tctgtcagaa gctcggtgaa gtcggcagac
     2641 agacaccgta acagataatg ctgacgaaag attcccagag gtgggcgttg tgtcctatat
     2701 gccggaagtc tataaaatat ctatggttag aatgtagatg agaggtctac tgacgaatcc
     2761 ttgaatgtac aggtctatat cggggctgga tagaaatgtc cagtgacaca aacagtgtcg
     2821 ctactgtggg tgagtaagac tccgcattta tgaaaatcca tataacgtta aaggcgttat
     2881 caaggtagca ggcttataga ggaaacctaa ggatatatgt acagatagtg tgtttggaac
     2941 gttgaaagct gacaacatgg agattttact gtctgtgaag tgttcacatc agaaagttat
     3001 tgtgagatta gcaatgatat aaggtgtgtt aatgtcagtg agagtggtgg cacagtacct
     3061 atgaaaccgt gataacaagc ggtggaggga tagccactag tcatatgaag aatagaatta
     3121 ctaagaaaaa tggtatgggt tcgagtaaga ctaagagatg tacttgctcc tgcaaacaag
     3181 gagggtacag accatgctaa agaaaaacaa gctccgctat aacgagtatt atgatatgca
     3241 acatatttat gacgagttat acgcacagag caagaacggt aataactttt ataagttact
     3301 tgaaattatt ggttcggaac aaaatatttg tttagcttac cgtaatctga aatcaaacag
     3361 tggcagtaaa acggctggaa cggatggaat gacgatagac gatataaaac aactcagcaa
     3421 tgctgagatt gttgctaccg ttcgggaaag ccttagcaac taccgcccca aatcagtcag
     3481 acgtgttttc attccaaaag ctgggagcga taaaatgcgt ccattgggga ttccgtgtat
     3541 ctgggacagg ctggtacaac aatgtatcct gcaagtccta gagccgatat gcgaaccaaa
     3601 gttccataat cattcctatg gattccgtgc gaatcgaagt gcccaccatg cagtcagcag
     3661 agtaacgaca ctgataaatt tatcaaaata tcattactgt gttgacgtag acattaaagg
     3721 atttttcgat aatgtcaatc atggaaaact attaaaacaa atctggacac ttggtattcg
     3781 ggataaaaga ttgatttgca ttatcagcaa aatgctgaaa gccgaaattg acggcgaggg
     3841 tgtcccagaa aaaggaacgc cacaaggcgg tctgttaagt ccactgctct ccctcatagt
     3901 ccttaatgaa ttagactggt gggtaagcag tcagtgggaa acattccagc cgaagaaccg
     3961 cagtaaaaat gggtggcttc aatacgcaaa gaaatatacc aaactgaaaa gcggttttat
     4021 tgtacgttac gctgatgatt ttaaaatcat gtgttcaacc tatggagaag cacaaagatt
     4081 ttaccacagt accgtagatt ttcttaataa aaggctgaaa ttagaaatca gtccagagaa
     4141 atcaaaggta gtaaatctga agaaaaattc atcagatttt ctggggttca aaatcaaagt
     4201 cattccaaaa ggaagaacga aacacggtta tgttgccaaa acagatatga accaaaaagc
     4261 attaaagaaa gcgaaaacaa atctaaagtt aaaggtaaaa gatatagcca gacatacgac
     4321 tggcttcaat atcagcagat ataatctgac agttatcggt atgcagaatt actactgcat
     4381 agcaactaac gtatataaca acctgacaga agtaagttac gctctcttac cgaccatacg
     4441 aatccgttta aggaacattg caaaatcagt tccatttgaa agtacaagtt cagaatttca
     4501 gagcaggaca aaaggaatca gaccaaaaac aaaaatagtg atgattgcgg ataatccgct
     4561 attgccgatt caaggagtac aacacaaaaa tcctatgaat ttctcgcaag atatttgtaa
     4621 ttttacaaag caagggagaa acaaggtgca tgaggacgtt gtagttgtaa caaaagaaga
     4681 aatccgagcg ttgttggaaa atgaaaaccc tgccgatagc gtagagttta acgacaatcg
     4741 tatttcggca tatatcgcac aacaaggcaa ctgctatgta atgaatcgtc gaggaactcc
     4801 gtccactctg atatgtattc acaaatcaga caaaggagat aaccttgaca gatattctaa
     4861 tctggcattt gtggaaattc ccattgcaaa ggcaatccta acagaatcag cagacgaagc
     4921 aaaatcactg ttaaaaggtt atgttcttaa tagtcagcag aagaaaaaac tgaacagaat
     4981 acggacgaat tacggatacc agacaataac tgggaaataa cttataaatc tatttggata
     5041 tgatggaacg ccgtatgaag cgaaagtttc atgtacggtg tgaagcgggg gaaaacttgg
     5101 agataacatc aaaaagttac ctatcgctat agcgaggagg gtggaaagaa accttgccag
     5161 aaattgcgga tgaaatcagt atcctcaact tgacgagtaa gtcagaaaat caaggtctgc
     5221 ttgatcctta tgtgattatg aagaaaaaga aagactcgga aagtttagca attgatattc
     5281 tgactttttt aacaggaatt tcaagtcgag acggggagaa gtttcctgtg ttacgtaaag
     5341 ccattcgaac ggtgactcaa agtgatcaac gggggttatt acaagttatt aacgaattgc
     5401 gaaaagacgg gtcaccaatc gcggaaaata tcgccgatca tatcgagtcc tttaccgatt
     5461 atgattttgc tcatttgctt ttctcagacg gctcgattac ccaatcgatt agtttagata
     5521 aacagctaaa tatcattcaa gtggcggatt tggttttacc ggatgcagaa acgacttttg
     5581 aagaatatac caccatggaa ttgcttagtg tttcgatgct aatcgtcatt tccacttttg
     5641 ccttggactt tatccatagt aaccgagaaa tcttcaaaat tgtcgatttg gatgaggctt
     5701 ggagttttct tcaagtggct caaggaaaaa cgttatcgaa taagcttgtt cgtgctggtc
     5761 gggccatgaa tgctggggtc tatttcgtca ctcaaaatag cgacgattta ttagatgaac
     5821 gtttaaaaaa taatatcggg ttgaagtttg cttttcgcag taccgatatt catgaaatca
     5881 agaaaacctt ggaattcttt ggtttggatc aggaggatca aagcaatcaa aaacgcctgc
     5941 gagatttgga aaacgggatt acgtccatat aattgtgtaa aagtgaagca tcaaaaaagc
     6001 cattgatctc agttaggagt acaatgtttt ttgcagaaaa catactcgaa taggactgat
     6061 atcaatgact caagtacatt ttacttttga aagcgaagaa attcaagcaa ttataaacga
     6121 aagcggtgcg aatgataccg caaaaacact tatgactatc atgtttaatc agctgatgga
     6181 agaacaacga aatcaatata ttcaggcaaa tgcctatgaa cgttcagaag aacggcaatc
     6241 acaaagaaat ggttactacg atcgttcgtt tacaactcga attggtacgt tagaacttca
     6301 tgttccacga accagagacg gtaagtttag tcctactatc tttgaaagat atcagcgaag
     6361 cgaaaaagct ttgatcgctg ccatgattga aatggtcatt tctggtgtct ccactcgcaa
     6421 agtaactaaa acagttgaac tgctaacgga tggtgcgact gtttctaaat cattcgtttc
     6481 aaatctgatg aaacagttgg atccatttgt ttttgagtgg agaaaccgga gcctagaagg
     6541 ctcggaatat ccatttttca tgtgtgatgc cctgtatatg aaagtaaggg aaaatcatcg
     6601 tattgtctca aaaggtgtat atattggtat cggcattgat tctgatggac gacgtacgat
     6661 tcttggtttt gatgttcaag acggcgaatc ggaagataat tgggataccg tttttcaatc
     6721 gttcgttcaa cgaggtttgt tcggtgtaaa actggtcatt tctgatgcac acaaagggtt
     6781 agttaaggct gttcgcaaga actttttagg tgcgagctgg caaagatgcc aagcccactt
     6841 tttgagaaat atttttgata agctgccaaa gaaagtttct tctgatgtaa aagacgagtt
     6901 gaagagtatc tttaaagcct ctgaacttga attgacacgt gaaagaaaag agcacttctt
     6961 ggaaaaatat ggttgtgatt caaaattaag tgctgcttgc gacattttag aaaacggctt
     7021 tgaagatgct attcaaattt tgtcttttcc tgaaaatatc cgccgaagga ttcgtacaac
     7081 caatgtgttg gaacggttga acgaagagat tcgccggaga gaacgagtga ttcggatttt
     7141 tccaaatatc aattccatca ctcggataat tggaacactt ttgatggaaa aagacactga
     7201 gtggctggct tctccacgaa aatatttaga atttaattcg aataacattt agcaccatga
     7261 acctgtgcga gtctcttctt tctgaggaga gacatcaaga gtcgctgcgc tactgctcct
     7321 gacctctctc ctcagaaaga aaggcatggc agttaggttc acaacgcaaa acattgtgct
     7381 tttgatgagg ttgaactttt acacaagatt gtggacttga ctgaaaacgg tcaatgcttg
     7441 atgcaagact tgtatgggcg agtaggtgtg gttcaagttc atccagtctt tgaagaactc
     7501 ttccatgctt ttgacactcg tccgccggtt caagagagaa tggagtgagg ggatgaaaaa
     7561 gaaacttttt cggattgtgg gaatcgtttt tattagcatc gtcctattga cagttctatt
     7621 gagtttagtc ggtacggtag ctgaggcgac aggtctggtt gatgatacag ttgaagccgg
     7681 aaacctttat tccaaatatt ctctgaacaa ttatcaactg gatttctttg tggatagttc
     7741 ttgggattgg ttaccttgga attgggggga tggcttaggg aaaagcgtga tgtatggtct
     7801 ttatgccatt accaatttta tttggacagt aagtctgtat ttatcaaacg ccacgggcta
     7861 tgtggttcaa gaagcttaca agttggattt catttcggat acggctaaaa gtattggaaa
     7921 gaatatccaa actttagccg gtatcactga aaatggatta cagacttcgg gattctactt
     7981 tgggttccta ttattgatga ttttagctct aggagtttat gtggcgtata ccggattatt
     8041 aaaacgagaa actaccaaag cagtccgaac cgtcattaat tttgtgatga tttttctact
     8101 ctcgggttct ttcattgcgt atgcgcccac ctatattaca aaaatcaatg actttagctc
     8161 agacgttagt gaagcggctc ttagccttgg aaccaagatt gttgtaccta attcagaaag
     8221 ccagggaaaa gatagtgtgg atttgattcg agacagttta ttttcgattc aagttcaaca
     8281 accctggtta ctattacaat tcgatgattc caatatagaa gaaattggcg agggtcgggt
     8341 taataagatt ttatccgtga gtccggatga aaataagggt aaggatcggg aagaagcagt
     8401 caaagcagag attgaagata atgacaatgc gaatctcagt attaccaaga ccatgaaccg
     8461 tttaggaacc gtggtctttt tagtgttgtt taatattggg atttcctttt ttgtgtttct
     8521 tctgacaggg attatgttgt tctcacaaat ccttttcatt atctttgcga tgtttctgcc
     8581 cattagtttc ttattgtcga tgttgccgac gtatgagagt ttgggcaaaa gggcgattat
     8641 tcgtttgttc aatacgatta tgatgcgagc gggagttacc ttagtcatta cgactgcatt
     8701 cagtatctcc acgatgtttt tcaatatttc ggccacctat cccttcttta tggtagcttt
     8761 tctacaaatt gtgacctttg ccggtatcta tttcaaatta ggtgacatta tgagcatgtt
     8821 taaccttcaa agtaatgata gtcagtcaat gggaaggcga gtcatgcgga aaccgcaaat
     8881 gttgatgaat cgaaaattgc gacagcttaa tcgaaatgtg ggtcgaactt ttgcttttgg
     8941 tggcggtgca gcagttggtg ggaaactagc taccagttct acaaaaggga aaggtagttc
     9001 aaaaatcaat agagtgtcca atagaaagaa ccattccgcg agaagcatta acgagctaaa
     9061 ttctgatctg aaaaaacaag aacaaccagt gggcacgaag aaagaatcac gaataaattt
     9121 agttggtaga aaagttggaa aagtactcga tacaaaagaa atcgtgaagg acaaagcgaa
     9181 acaagcaaaa aatcaagtaa ccgatgcacc aactaacttg aaatacagcc ttcataaagg
     9241 catggaaaag acgaaaaaag taccagagga ctttaagcga gggcctttcg aagaaaaagc
     9301 aaatcgaggg gagttgcgag aaaaacaacg acaacgaaga gacgcaaaaa tggatgaaaa
     9361 acgcaaagta ttgaacgaag catggaatcg tcatgagaaa ggaagggaaa atgttccgat
     9421 aaaaaaggag ataaaaccac agaaaaatga actaccagaa aataggtctt taaaaccaag
     9481 ggtacaagta cattcgaatc ctgaaatcaa acggaaactt tctagtcaag aagtcattcc
     9541 aaacggaagc actcagccac taagcaagaa caattccttt caacaagtga aacagagatt
     9601 cacttttcca aagcgaacca atcaacaagt acaaagaaaa aagaacactc gcccaaaacc
     9661 aaagccaggt gagaaaagat gaacttaaaa aaattgagta ttatttcgtt aaccttcctg
     9721 accatctctt ttgtgggcat cttgatgtgt gtgggtttgt tgtttggtga ggacggtgag
     9781 ggaagtggta gttctggaac ttctcctgga ggtagtagtg tctcagaagt agttttgaaa
     9841 caccgttcaa tggtggaaaa gtatgccaaa gaatcagata taacagagta tgtttcgatt
     9901 cttttggcga ttattcaagt cgaatctgga ggtacaatgg aagatgtcat gcaaagttct
     9961 gaatcagcag gtttatcacc gaattcatta agtaccgagg catccatcaa acaagtgtgt
    10021 ctctattttt cgtctttggt taagtgttcc aaagaactag gatgcgatca agatagtatc
    10081 attcaggctt ataactatgg tgacgggtat ttggattatg tcgcgaaaaa cggaaagaag
    10141 catagttttg ctttagccga atctttttca aaagaaaaat cgggtggtca aaaagtcgat
    10201 tatcccaatc ccatcgcgat taaggaaaat agtgggtggc ggtataacta tggcaatatg
    10261 ttttattgct tattagtgaa gcagtaccta actacaacac agtttgatga taaaacagtc
    10321 caaggaattt ttgacgaggc gttcaagtat gaaggaacgg cttatgtatt tggtgggtca
    10381 agtcccggaa ctgggtttga ttgtagtggt ttaactcaat ggtgttatgc caaagttggt
    10441 ttgaaactcc ctcgggtggc tcaggatcaa tatgatgcta tgactcatat tgacttaaaa
    10501 gatgccaaac caggcgattt gattttcttt cattcaactt atgatgccgg tacttatgtg
    10561 actcatgtgg gcatttatgc aggagagaac agaatgtttc atgcaggaga tccggtgggt
    10621 tggaccaatc ttaccgatag ctattggcaa cagcacatta ttggtgctgg tcggtataaa
    10681 caatagaaaa gaggaatgga aaatgaagat aaaaattgag cgaagtaaca aggaaaagat
    10741 tccaaaaaag aagaaagaac gagtagtatc cgtgggcaag catcgaaaaa tggtgcttgc
    10801 cctttggctt ttattgtctt gtagccttgc ttttggaatc tacaaaaact tcacggccat
    10861 tgatcagcat acaactcatg agaaagtggt cgtgaaagag aaaatagtga atacctctgg
    10921 agtagaaaat tttactgtag attttgtgaa agaatatttt tcatggaaga ataacaagga
    10981 ggtaattgaa aagagaatga ctaatcttga gcagtacctt acagaagaag ggctcgcttt
    11041 gagccaagat atggttcgag cggatattcc cactagttcc gaggttgtat cagtaaaaat
    11101 tctagatgtt gaaaaacact cggaagaatt tgtcgtttca tttgtggtag atcaaaagat
    11161 tacagaaggg aaaaagacac aatcaatttc ttctgcatat cgtctgatga tctttgaaga
    11221 tgaaaaaggc aatcatattg taacgagttt acccactatg attggtaagc cagaaaaggc
    11281 aaaacataaa gtaagacaag taaaaagtga ttcaaaaatt gattcaaaga caacggaaga
    11341 aatcaccgag tttttagaga cgtttttcag gctttatcca gcagcctcag aaaaagaact
    11401 agagcattat gttgaaaaag acgcgatgca accaattaat actaatttga aatttgtgga
    11461 attagtgaat cctatttaca aacaaaaggg taaagcgatt caagtgattg ttggtgttga
    11521 gtatcttgat gagttgtcca aaacaacaag tgtttttcag tatagattaa aact
//
LOCUS       DAHHZJ010000091        11533 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.092, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000091 DAHHZJ010000000
VERSION     DAHHZJ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.092"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(158..643)
                     /locus_tag="NBL02_001835"
     CDS             complement(158..643)
                     /locus_tag="NBL02_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HCD2572515.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    733..1078
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1123..1764
                     /locus_tag="NBL02_001836"
     CDS             1123..1764
                     /locus_tag="NBL02_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCD2572516.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            1783..2418
                     /locus_tag="NBL02_001837"
     CDS             1783..2418
                     /locus_tag="NBL02_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCD2572517.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2436..3251
                     /locus_tag="NBL02_001838"
     CDS             2436..3251
                     /locus_tag="NBL02_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCD2572518.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3570..5006
                     /locus_tag="NBL02_001839"
     CDS             3570..5006
                     /locus_tag="NBL02_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCD2572519.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5195..7642
                     /locus_tag="NBL02_001840"
     CDS             5195..7642
                     /locus_tag="NBL02_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCD2572520.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7872..8939
                     /gene="rlmN"
                     /locus_tag="NBL02_001841"
     CDS             7872..8939
                     /gene="rlmN"
                     /locus_tag="NBL02_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HCD2572521.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(9132..10235)
                     /locus_tag="NBL02_001842"
     CDS             complement(9132..10235)
                     /locus_tag="NBL02_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HCD2572522.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10788..11336
                     /gene="aac(6')"
                     /locus_tag="NBL02_001843"
     CDS             10788..11336
                     /gene="aac(6')"
                     /locus_tag="NBL02_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HCD2572523.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         3826 a         1947 c         2493 g         3267 t
ORIGIN      
        1 atatggattt ggctaactat ggaagaatac tcgtattagt tttcagcgtt catactagtt
       61 gttacattgt ttgattggcc aaaagaattt ctgatatatt ttgtgctaaa taaagaaaaa
      121 gacgcttgaa ttgggaaata agaaaactac tctatcatta caaattttca taaactggca
      181 gatgttctaa gtaaacaagt atcttttcaa agtcggaaaa atccataatc aacgtagtgg
      241 tattgatatt tggatgaaag cctacggtat cttgacgatc aatagcagca tcgatcacga
      301 cttggatttt tttctctttg tcgtgcatca atgctaatgg agtcactgta cctgctggaa
      361 cgcctaggag gtcaggaagc tgttcttcag aaacaaaaga taagcgcttt tcttttaatt
      421 gttcagctag ttgttttaaa tcggcgtgct tttcatcagg caagatgaca agatagattt
      481 gttttccttt tcttgatttc aataatagat tttttacttg cggaccaggt agggaaaaag
      541 ggacattttt gactgacgta atcgcaggat gatcgacttt ttgaaaagag atacccagtt
      601 tttttagtag ttcataagct tcttgttcgg ttgcacgact catcgcgcat caccctcctt
      661 tttttattag tgtatcataa aaaagagttg acaaatgaaa aataatcatg taaattacac
      721 gtaaagttaa aacaaatgac atcgaaaaga agagtagccg ttaggtgttt ttagagagtc
      781 tctggttggt ggaaagaggc agcagttaac cgtgaaacac atcttggagt cgattttctg
      841 aagcgtatag taggaaaatc cggggagccc gttacagctg cggtcgttgc tagatgacca
      901 gataaggctg gcttttccgt gaggagtcgg taaaaaaagg tggaaccacg tgattaacgt
      961 cctttgatct tcggatcaaa ggactttttg ttttcatctg aacggcaacg actgtgtgag
     1021 gttttttctg caaggaaaaa ataaacagag gtggaaccac gtataatacg tcctcttggc
     1081 atttgccaag aggatttttt tattttaaag gaggaaaaga agatggagta tttgattgaa
     1141 gttatgccgc aattattagt aggagcactc acaacattga aattattttt atttaccttg
     1201 attggctcat taccattagg gattgtttta gcattaggat tgtctttgca ttttaagccg
     1261 ctacgctact tgcttcaaac atatgtatgg gttatgcggg gaacaccgct gttgttgcaa
     1321 ctgattttta ttttttatgg gttgccgacg attgggatcg tttttgatcg tttcgaagca
     1381 gcactcattg cctttatctt aaactatgca gcttattttg cagagatctt tcgtggaggg
     1441 atacaatcga ttcctcaagg tcaatatgaa gcagcgcaag ttttgcaatt aagtcccttt
     1501 caaacggtga cgaaaatcgt catcccacaa gtgacaaaga ttgttttacc ttctgtaggg
     1561 aatgaagtta tcaacttggt caaagattct tccttgatct atatattagg tttaggtgac
     1621 gtaatgcgtg ctgggaaaat cgcgatggaa cgcgacgcga ctttactgcc tttgctaggc
     1681 gtggcaatca tctatcttac attgactggt attctaacaa tcataatgaa acaagttgaa
     1741 ggaaaattaa attattaccg ctaactgata ggaggaagaa atatgattga attgaagaaa
     1801 gtgaacaaag catttggtga aaaaaaaata atcaaagatt tgaatctaat cattccagat
     1861 gggaaaacat tagctatcgt aggtccttcc ggtggcggta aaaccacgct tttacgtatc
     1921 ttggctggct tagaaagttc tgacagcgga gaattttatt tagacggtgt accgttcaac
     1981 ccattcgagc ttaaaatgaa agaacaagtt gtaggagtcg tattccaaga cttccagcta
     2041 tttccacatc tgaatatttt tgaaaacatt accttagcac caaaaatggt attgaaacag
     2101 acaaaagaag aatacaccaa aaaagcagaa gaattagcgg cttcattagg gattagtgat
     2161 ttattgaata tctatcctta tcagctttct ggcggacaaa aacagcgggt agcgattgcc
     2221 agagcattgg ctatgaatcc acgggtattg gcttatgatg aaccaacaag tgctttagat
     2281 cctgaattac gccagcaagt cgagcgtttg atcctatcat tgaaagaaga tggggtaacc
     2341 caaattattg tcacccatga tttagtattt gcagaaaatg tcggtgatca attactaaaa
     2401 gtttccccaa ttcattaaga aaggagaaga tagagatgaa aaaaaccagt tttctattcg
     2461 ctttgatcgc aggtcttctt ctttttgcag gctgttcgaa caaaaaaact tcagcagata
     2521 attgggcgac tatcaacgaa gagaaaaaaa tcgttatagg tttagacgat acatttgttc
     2581 ctatgggatt tagagataaa gacgggaagc tcacaggatt tgatattgat ttagccaaag
     2641 cagtctttga acaatatggg attacggtag actttcagcc cattgactgg tcaatgaagg
     2701 aatttgaact gaacaatgga acgatcgatt tgatatggaa cggctattcc aaaacgccgg
     2761 caagagaaaa gaaagtgcaa tttacaaaac cttatatgga aaacgatcaa gtgctgatca
     2821 cacctaaaga atcaggaatc acaaactttg gacaaatgaa taataagaga ttaggtgcgc
     2881 aaaatgggtc atctggttat gatgtattta cgaaacaacc agaagtatta aaagagatag
     2941 tcaaaaacca agatgctgtt ttgtatgata gttttaatga agcattgatc gatctgaaaa
     3001 gcagacgggt cgatggtctg ctgatggata aagtatacgc agactattat ttgaaacaaa
     3061 gaaacgaact agaagacttc aacataacaa aaggttctta tgagagtgag gattttgcag
     3121 ttggtgcacg caagtcagat agtacattag tgaaaaaaat caatgaagcc ctagatcaac
     3181 tagagaaaac aggtgaattc caaaaaatat ccgataaatg gtttggcgaa gatgttacgc
     3241 cgagaaatta acaaataggg gactgtgaca aaagacgtgt cacagtccct ttttccgaat
     3301 aaacagtgtt ctcctgccaa ctccttgtga ctcggagctg aatggcaggc tcacaacctc
     3361 ttggtaaacg gtgttcaaaa gccagttttt cggtattaag ccaaatttat gcaaaaatgt
     3421 agaaagccat tttcacaaaa tttttcttac tactcgaaac taagcgactt tttcacaacc
     3481 tcctttacat aattttattt tgttgtggaa ggggtttcgt gttattctaa aagaggaacg
     3541 attgaaggtt ataggaaagg aagagatgaa tggaacacaa agaacttacc tcatttccaa
     3601 aagattttct gtggggctct gcttctgcag cgtatcaagt agaaggagct tggcaagaag
     3661 atgggaaagg agaatcagtc tgggatcgat ttgtacggat acctggaaag acatttaaag
     3721 gaacgaacgg tgatttggca gtagaccact atcatcgtta taaagaagat attgctttaa
     3781 tgaaagaaca aggtctgaaa gcttaccgct tctctgtagc ttggacacgg atttttccta
     3841 atggacgagg agaagtcaat caagctgggc tcgcttttta tgagagattg atcgacgaat
     3901 tgattgaaaa cgatattgaa cctatgttga ctttatatca ttgggatctt ccacaagcat
     3961 tgcaagatga atataatggt tgggaatcac gtcagatcat tgaagatttc actcattatg
     4021 cagaaacatt atttgaggca ttccgcggga aagtccatta ctggattagt ttgaatgaac
     4081 aaaatatctt cacttcatta ggttatttgt tagcagcaca tccgccaggt gtgacagacc
     4141 cgaaacgaat gtatgaagtt aaccatattg ctaatttagc aaatgcatct gtcatcaata
     4201 aatttcacga gttagaaatg cctggaaaaa tcggaccaag ctttgcgtat acgccaaatt
     4261 atccaattga cagtaatcct gaaaatgttc tggctgctga aaatgcagaa gacctgatgg
     4321 ccaattattg gatggatgtc tatatgtggg gaaaatatcc gattgctgcc atgaggttct
     4381 tagaagaaaa aggatgggca ccaacgattg aagctggcga tgcagaattg ctagaatctg
     4441 ctaaaccaga ttttcttggg atcaattact atcaaacggc gacgaatgca ttcaatccat
     4501 taaacggagt tggtgctgga aaaatgaata cgacaggaaa aaaaggttca tcagaagaaa
     4561 ctggagttcc tggaatgtat aaaaaagttg agaatccatt tgtcgaacga acaaattggg
     4621 actgggagat tgacccagaa ggattaagga tcggactgcg tcgtatcaca agccgttacc
     4681 gcatgccggt actgattacg gaaaatggct taggagagta tgacaagttg acagaagata
     4741 aacaaatcca tgacgactat cgaatcaatt acttacaaag ccacgtcaaa gccatcaaag
     4801 aagcaatttc ggatggagca gaagtcctag gctattgcac ctggtcttat acagatttgc
     4861 ttagctggct gaacggttat caaaagcgat atggatttgt atatgttgat caagacgaaa
     4921 cacaagaagg atctttggaa cgatataaga aagacagtta ttactggtac caaaaactga
     4981 ttgaagaaaa tggagagaac ctttagttca catacttaaa acaagaaggt acggctggat
     5041 atggccgtgc cttttcttga taaaatcatt tagaaattaa gaaaaactgt aaaataagct
     5101 gaaaaatgaa agaaagcgtc agttctttca aacaaaactt tagttccctt actttttttt
     5161 gtataatgaa acttgatgaa atgagggata ataaatggca caaaagaaac ggcctactaa
     5221 aaaaaggaaa aagacgaaaa aaacgaaaaa gcaaaaacag cagcaggaac aaatgctgac
     5281 gattggtgtt gggtttgcct tgattttatt tgctgtcttc ggctttttaa aattaggttt
     5341 tttaggaata ttgattgcaa acggctttcg gatcattgca ggaaatactt accaaatcct
     5401 ctgtctttta ttagccgtat taggattttg gattgtcatt aagaatacag agttttcaat
     5461 tggtaaaaac cgacgctggt ttggcggcat actcttttat ttcggtatct tattattgtt
     5521 acatgcccat ttatttggca aactccatac tggggaacca aatatcatgg ggacgacatg
     5581 ggatttgtta gccagtgata taaaacaaag tcaagtagac aataacgttg gtggtgggat
     5641 gattggagca atcctttatc attttactta cttcttgatt gcacagcctg gaagttattt
     5701 agcagcagtg ctattactgg caggaggagc attcctgttc agtaatctgg aaggctatca
     5761 gctgttaaat ggtatccaat caattggaga acgagtacag gaattattgg aaggtgatcc
     5821 tgccaagcaa gctcgaaagc aagcggcaaa ggaagagaga atgaaacagc gcgcagaagc
     5881 gaaagaagca aggcgcttag ctgcaaaaga agctgcagaa aaagaagctg cagaatatga
     5941 gaagaaaaaa gcggtgagcc agcaaaggga aaatactgct gatgaatggc aggaaccaga
     6001 aaatacagag ccggagcagc taagttttgt tccaatcgac agttttcaag aaaatatcca
     6061 tcctgcgaat ctagagaaac cagtaccaga tacaccaaaa cagaccaata cagcagaagg
     6121 tttttcagat gagttgccag aggatgacgg tacctcatta gaatttgaga ttgaagcaga
     6181 acaggaaaat caagactatg aattaccgac agttgatttg cttgattcta taccaactgt
     6241 ggatcaaagt gatgagtata aaaaaattga aaaaaatatc ggcgtattag aacaaacttt
     6301 ccaaagtttc ggtgtcgatg ccaaggtcgt caaagccagc ttaggaccat ccgtcacaaa
     6361 attcgaagtc cagccggctg taggggtaaa agtaagtaaa attgtgaact taacagatga
     6421 tatcgcgctt gctttagcgg cgaaagatgt ccggatggaa gcaccgattc caggtaaatc
     6481 actgattggt attgaagtac caaatggaaa gatcagtatg gtttccttta gagaaatcat
     6541 cgaagcacaa ccaaaccacc cagataaatt actagaagta cctttaggac gtgatgtttc
     6601 tggacgggta caaacggctg atctttcgaa gatgcctcac ttactcgtgg ctggttcgac
     6661 tggtagtggg aagtcagtag caattaatgg gattatcaca agtatattaa tgcgtgctaa
     6721 gccccatgaa gtaaaactga tgatgatcga tccgaaaatg gttgaattga atatgtacaa
     6781 cggcatacct catctgttga cgccggtcgt gacgaaccct agaaaagcag cgcaagcctt
     6841 acaaaaagta gtacaagaaa tggaagaacg atatgaaaaa ttcgcggcaa ccggagtaag
     6901 aaatatttct ggttacaatg aatttgttca acagaaaaac ttggaaaacg gaacgaaaca
     6961 tccaacctta ccattcatcg tagtgatcgt agatgaatta gctgatttga tgatggtagc
     7021 cagcaatgaa gttgaagatg cgattattcg tttagcgcaa atggctcggg cagcggggat
     7081 tcatatgatc ctagctactc aacgtccaag tgtggatgtt atcacaggaa tcatcaaagc
     7141 aaatgtacct tccagaatgg catttgcggt atcaagcggt acagattctc ggacgatcat
     7201 tgattctaat ggggcagaaa agctcttagg gcgaggagat atgctattct tgccaatggg
     7261 tgagaataaa ccgatccgtg tgcaaggagc attcatttct gaccacgaag tagaacgggt
     7321 cgttcaattt gttacagatc agcaagaagc tcattatgaa gagaaaatga tgccgacaga
     7381 tgaagttgaa acgacaggtg ctccagaaca gccacaagat gagctgtttg aagaagcaaa
     7441 agcacttgtc gttgaaatgc aaacggcaag tatttccctt ctccaaagaa gatttcggat
     7501 cggttacaac cgagcagcac gtttagtaga tgaactggaa gctcatggag ttgttggccc
     7561 gtcagaagga agcaagcctc ggaaagtatt cattgagcag gaagaaacat cagaaacgtt
     7621 gccggacgat aatgacatgt aaaacttgtg tgatgaaggg ttgaaaaacg aataaccaat
     7681 aaaaagacaa ggcatgatga taccaaaaga aaaaatggtt tttttccatc ttttctaata
     7741 cggtatgatg tcttgtcttt ttagtttttg tgagattaaa gttctattcg atttatttgt
     7801 gaagtattgc attaaggaaa tgaatcatgt atagtatgta gcgaaacttt gagaaaaaga
     7861 gggaaaatac tttggaaaaa cagtccattt atggcttgac gaatgaagaa ttaatcaatt
     7921 ggtttataga aaacggcgag aaaaaattcc gagctgccca agtttgggaa tggttgtatc
     7981 aaaaacgtgt aagtaatttc acagaaatga cgaatctatc gaaacaattg atcgaaaaat
     8041 tatcggagca ttttatcatc aatcctctaa aacagatggt cgtccaagaa gcttcggatg
     8101 gtacagtaaa atatttgttt gaactccctg ataaaaacat gattgagact gtactgatga
     8161 gacaagaata tggcttgtca gtttgtgtga cgacacaagt aggatgtaat atcggctgta
     8221 cgttttgtgc aagcgggctg ctcaaaaaga accgtgatct gactgctgga gaaattgttg
     8281 cacagatcat gatggtccaa cattattttg atgagcgcaa tcttggcgaa cgcgtttcac
     8341 atgtcgttgt aatggggatc ggtgagccat ttgataatta cgacaatgta atggactttt
     8401 tgcatatcat caatgatgcc aaaggattgg caattggtgc tcggcacatt acagtttcca
     8461 ctagtggatt agcacacaaa atcaaggaat tcgctaataa cggcttgcaa gtcaatctgg
     8521 ctatttcctt gcacgcgccg aataatgagg tgcgaacatc gatcatgcgg atcaaccgaa
     8581 gtttcccaat tgagaaattg atggaagctg tggatgagta tttagagaaa acaaaccgtc
     8641 gaatcacgtt tgaatatatc atgttgaatc aagtaaatga tagaccagag catgcgcaac
     8701 aattagcaga tctattaaaa gataagaaaa aattagcata tgtcaacttg attccatata
     8761 atccagtcag tgaacatgat caatattctc gcagtccaaa agctgatgta ttgagatttt
     8821 atgatgtatt gaagaaaaac ggcgtaaact gtgtgatacg taaagaacat ggtacagata
     8881 ttgatgcagc ttgcggacaa ttgcgaagca agcaaatgaa aaaaacagtt gccaaataat
     8941 cgtatcctac tcgttttttc aattaccata aaaggagcag ttttcttaat taaggaactg
     9001 ttcaacttaa ataagagaaa agattctata gcgaaagcta taaagctttt tttgacaaga
     9061 aaaaccctca tcaagcaaag aaatgtcctc aatttgatga cagcctgctt gatgggggtt
     9121 ttagcaatta tttaactgat tttcttatat ccttgctggc tttcttgtgt agcatgataa
     9181 gtttctacgt caagaatggc ctgtttaaat ttttctcggg tgatttcata aggcacatgt
     9241 ttaatatcat ttgttgccat ggctttatct aagattgctt ctaaatcggt cgtgacattg
     9301 agattcatgt cggttaacga tactggaagt tggttctttt tcataaacac atagatttta
     9361 tcaaacaaat cgaactgctg atccatcatc aataaggtca gtacaccata actgacagat
     9421 gctccgtgaa ggtatccctc cattttttct agaacagtcg taccgtaata gaatgagtgt
     9481 gccatagcag aattatagtt attgttaact aaaacagaaa ccaatccggt agtaacaatg
     9541 atatccgaaa cgacttgttc aattgcatat gagggacgat cgtcggcact atctttcaat
     9601 gcttgcgtac cataagtcaa gagacgatca atgcacattt ttccgatttg gattcctaat
     9661 ctatccgtgt agcctagatc atcgccacga gcagaaaaag tagattcgac ttctttggat
     9721 aaagcatcgc caatacctgc ccataagtat ttatcaggtg cttcggcaat aattgctgta
     9781 tcaatgaagg tgtgtacagg tggttttgaa ggaaagaaga gtccgtccat tacgccttct
     9841 tcgttataga tgacacatac cgcagtgacg ggcgaacagt tcgaaccaat agtgggaaag
     9901 gtgaagactg gtttagctag tttttcacca atgactttta cagtatcgca agcacggcca
     9961 ccaccgactg caaacaggac gtctgctttt tggacacttt catgctggat caactgttca
    10021 atatttccat aagttgaatt acccccgtac cagagactgg cgacaacctc tatttcctgt
    10081 gtcaaagcta cttttatttt gttttctgcc tttgaaagtg cagtcttccc tccaatcagc
    10141 actacttttg tcccatattc tttgacgatt ccatttgctt tttcatacgc atctgttcca
    10201 atggtataat ttggtaagaa aacactttga ctcatattca tttccttctt tcttcgttga
    10261 aacgggttaa aattttaaag agtgatcgat ctgactgcgg ctgtagatgg agtagaggtg
    10321 aacctaagac aggataagtg atgaataagg tggcatgaac accttgaaat catcaataag
    10381 tatcctaaaa acgaacaagt acaaaaacat tgaaaacgac aataatgaat ttttgttcct
    10441 cttagtattt ctaccgattc cctttgcttt tttgtaatta tctaaaaatt ctaaaaatta
    10501 agattattat atgaaaacag gttttatttg tcaattcata attggttttt tttaaagcaa
    10561 aagataatct atatcatgat gaacaaacag agttttttcc tccaattgat ggctttcctg
    10621 atctttccta ttttgaataa agtgtttctg tataattttt ttattttata agtaagataa
    10681 ataatgaaag aatagagata ctgattggta gctaaagatt tttgagcgag aacatttcgt
    10741 tttttcaaaa ggactgttag aataagcaag aacaaggggg aagacgtatg ataatcagtg
    10801 aatttgaccg taataatcca gtattgaaag atcagctttc tgatttactg agactgactt
    10861 ggccggaaga atatggagac agctcggcag aagaagtaga agaaatgatg aatccagaac
    10921 gaatcgcggt agcagcggta gaccaagatg agttagtagg atttattggt gcaatccctc
    10981 aatacggtat cacaggttgg gaattgcatc cattagttgt agaaagctcc cgacgaaaga
    11041 accaaatagg tactcgatta gtcaattact tagaaaaaga agtagcttcc agaggaggaa
    11101 tcacgattta tttaggtacg gatgatttag accatggaac aacgttaagt caaacggacc
    11161 tgtatgagca tacatttgat aaagtggctt ccatccagaa ccttcgtgaa catccgtatg
    11221 aattctatga aaaattaggt tataaaatcg taggtgtctt accaaatgca aatggctggg
    11281 acaaaccgga tatttggatg gcaaaaacga ttattcctcg accaaattct caataataaa
    11341 aagaggttgt gacagaagta atcagcttca agaaataaga aggaatttac gaaaattact
    11401 tcttgaattt ttgtgaaatt ccaacttatt tccgaagaag ctacttttgg aacaccgtgt
    11461 accagaggtt gtgaaaaagt cgcttagttt cgagtagtaa gaaaaatttt gtgaaaatgg
    11521 ctttctacat ttt
//
LOCUS       DAHHZJ010000092        11523 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.093, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000092 DAHHZJ010000000
VERSION     DAHHZJ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.093"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(34..2109)
                     /locus_tag="NBL02_001844"
     CDS             complement(34..2109)
                     /locus_tag="NBL02_001844"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCD2572524.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2111..3028)
                     /gene="glyQ"
                     /locus_tag="NBL02_001845"
     CDS             complement(2111..3028)
                     /gene="glyQ"
                     /locus_tag="NBL02_001845"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCD2572525.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3412..4224)
                     /gene="recO"
                     /locus_tag="NBL02_001846"
     CDS             complement(3412..4224)
                     /gene="recO"
                     /locus_tag="NBL02_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCD2572526.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4367..5266)
                     /gene="era"
                     /locus_tag="NBL02_001847"
     CDS             complement(4367..5266)
                     /gene="era"
                     /locus_tag="NBL02_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCD2572527.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5281..5676)
                     /locus_tag="NBL02_001848"
     CDS             complement(5281..5676)
                     /locus_tag="NBL02_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HCD2572528.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5660..6136)
                     /gene="ybeY"
                     /locus_tag="NBL02_001849"
     CDS             complement(5660..6136)
                     /gene="ybeY"
                     /locus_tag="NBL02_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCD2572529.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6153..8345)
                     /locus_tag="NBL02_001850"
     CDS             complement(6153..8345)
                     /locus_tag="NBL02_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HCD2572530.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8369..9340)
                     /locus_tag="NBL02_001851"
     CDS             complement(8369..9340)
                     /locus_tag="NBL02_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCD2572531.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10045..11424
                     /gene="brnQ"
                     /locus_tag="NBL02_001852"
     CDS             10045..11424
                     /gene="brnQ"
                     /locus_tag="NBL02_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCD2572532.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
BASE COUNT         3094 a         2422 c         2117 g         3890 t
ORIGIN      
        1 tgcctgttct ctggtttatt tacatattag aaattatttt gtgatgattt gatctaaact
       61 tgctagtgac aaagccatgg cagcaatttg acttaattgc gttaatcggt tcttacgtac
      121 tgctggttct tctgccatga ccatcgtctg atcaaaataa ttttcgatca gtgggcgaag
      181 gcttacaagc tgatcataat tttcgttgat cgtttggtca gcaaagcttg ttttcattgc
      241 ctctactgct ttgtgcagtt ctttctccgc ttcgttttcg aacagttcag ggtctacttc
      301 tgattgtttt tcaagctccg cttttttcgc taagttcacg acacgtgtca aagcttccat
      361 cgatggtttg aagtcttcat ctgcaagatg ctgcttgaac aattgtgctg ctgcaaatac
      421 ttttgtcaga tctttttgtt ctgcgttcaa aacagcttcg ataacatcat gacggatatt
      481 cttcgttgtc aataattggc gcagacggcc tttgataaag tcgatcacct gttgttgtcc
      541 cgctgacaag ccaatgccaa aacgatccgt atctttactg ataacttcac taatcgtttc
      601 ttgcagaaca gacaaaggaa aggcccattt tttcgattcg atgattcgaa cgacaccata
      661 tgcttgacgg cgcaaagcat atggatcgtt tgagccactt gggatcatcc caactgcaaa
      721 gaaagaaaag atactatcta atttatcagc aatcgctaaa acagaaccta ctgtagtctc
      781 aggtagatct ccttcactgg atataggcat gtaatgttca cggattgctg ttgctacggc
      841 tggtttctcg cctttaagca gagcatattt ttctcccata atcccttgta gttcagggaa
      901 ttctcctacc atgttggtta ctagatcaaa tttatagatt tcagcagcgc gttgcagatc
      961 catcaattct tcctcagata gtcctacttg ttcgcctatg atgcgagcga tgacttgtac
     1021 ccgttgcatt ttctcataga tgctaccgat tttttcatgg aaagtaacag tttttaattt
     1081 ttcaacgtat tggtcgattg aagctttctt gtcttcatta taaaagaatt ccgcatcttc
     1141 taaacgagca gtcaatactt tttgattgcc tcgtaccaca ttttccaatt ttacccgatc
     1201 gccattacga gcagaaataa aatgaggaag caacatacct tgatcgttcc gtacttcgaa
     1261 ataacgttga tgttctttca tagaagtgac tagtacttct tcaggaacag aaagatattt
     1321 ttcctcgaag tctccagaaa agacagttgg atattcgact agattgttga cttcttctag
     1381 taaatcttca tctagatcaa tgatccagtg atttttttct gctaattcgt ttgcttgtgt
     1441 aacgatcatt tcttgtcttt tttgcggtga agcaataaca aattgttcat ttaatttttc
     1501 ttcgtattct tttgcatgcg caaatgttac atcttcgcct aagaaacgat gacctcttga
     1561 agctctgtct gttttgacat ccaagatctc aaacgggatt atttcgtcat ctaacaatgc
     1621 cacgatccag tgaatagggc ggatatattc aaaatcgtat tctgcccaat gcatcgttac
     1681 tgggaaagtg aggcttttaa tgacatctgc taaacctgtc aatacttctt ttgcttcttt
     1741 gccgtgagtg tatttcgtta catagacata ttccacgcct tttaattctc taaaagtgat
     1801 agcatctgtc gtcagacctt gtccgcggac aaatccttca gctgctttcg tccagtttcc
     1861 atctgcgtcc aaagcaattt ttttggctgg tcctttgact tcttcttcgc tgtccgcttg
     1921 tcgttctgga atatttgtta ctttgattgc taaacggcgc ggtgtggaaa atgtttcgat
     1981 cgtatcataa gtcaattggt tttcatctaa aaattttgct gttttttctt ctaattgttt
     2041 catgcttggt gtgactacat gagcaggcat ttcttccagc ccgatttcaa gtaataagtc
     2101 ttttgccatc ttattcagcc tccttcacgt tttctggatt tgctgtgttt ttattcaata
     2161 aagggaatcc taatttttcg cgttctgcca caaagatttt ggctacagca cgagccatgt
     2221 tgcggatacg ggaaagataa cctgctcgtt ctgttacaga tactgcaccg cgagcatcta
     2281 ataaattgaa tgtatggctg catttcaaaa tataatcata cgctggatga acaagatttt
     2341 catcgataca gcgtttagct tctttttcaa aacggtcaaa attgcttagt agtaagtctt
     2401 ggtcgcttac ttcaaaagaa tatttggaat gttcatattc tggttgtttg aagatctcgc
     2461 catatttcac gccatcagcc cattctaaat catagacact ttcaacttct tggatataag
     2521 aagcaagacg ttctaatcca taagtgatct cagaagtaac tggtttacaa ggtagtcctc
     2581 cgacttgctg gaagtacgtg aattgtgtga tctccatccc atcaagccat acttcccagc
     2641 ccacaccggc acaacccatc gatgggttct cccagttgtc ttcaacaaaa cgaatatcgt
     2701 gttctaatgg atcaattcct aaaaggcgca agctttctaa ataaagctct tggatatttt
     2761 caggtgaagg tttcattacg acttgaaatt gatgatgctg atacaaacgg tttgggtttt
     2821 ctccataacg cccgtcagct ggtcggcggg atggctctac ataagcagca ttccatggtt
     2881 ccggtccgat tgcccgtaag aatgtatatg ggctcatcgt acctgctcct ttttctgtat
     2941 cataagcttg catcagcatg cagccgtttg atgaccaaaa tttttgtaat gttaaaatca
     3001 tatcttggac ggtcaatttt ttactcatct gttttgcccc tttcaatttt taagcgttgc
     3061 tgtcaataag aatacaggaa agaaaacgac aaaaagtccc tatgtctgat tacagacata
     3121 gggacgaata atttcgcggt tccaccctac ttccgaataa agattcggca gcttcattta
     3181 tcgtactcgc aagtgcctct tcaaaaatcg tttgggtccg gcttccactc tcccgaactc
     3241 gctaaaccac gtgtttttta ctgatcttgg tcaatgtacg tattcatttt gcagttttct
     3301 tgtttgtata atagatcctc tcagtgaacg atctatttaa tctacggtta ataatagacg
     3361 gaagtccctg tatttgtcaa atagttttga tttttttgtt gtatctttca tttattcttc
     3421 tttttcaggt atctcttttt caggcatatt tttctctggc gtctttaata cgtgtgccca
     3481 agacttcatt tgatcaatga actttttgct ttttagatga atacctacat attcatcata
     3541 caactggtca atgaggtcac ggattgcctg cttcgtttct tctttcaagt ggattccact
     3601 gatcttatca taagaaatag ccgaaaacat tcgaacgaaa taaacagcgc gaggatctgc
     3661 atgataacgg tgttcgtcca ttggccagtg tttctcgcat atcacaccgc catatttgga
     3721 agaatagtca aattttcctt ttgtttcccc gcatacgaca cagtgcgtcc aaattggcgc
     3781 gattccgaag cgttgcagta tttgaacttc aaagatattc gtgatgattt ctgcatcatc
     3841 tccttggtcc atccgctgca gagcttgttg cgtaaattgg aataaatgcg ggtcatattg
     3901 atgatcttcg attgctgcat ctactagatt tagcaaatac gtaccataac cgctcaaaaa
     3961 gatatcttct tggatgttcc gaaacgcatg gacgtctttg acactgctta aaaacgacaa
     4021 accttcactt cgaaaattac cgatatagac tgcttcggta aaggggagca aagccggagc
     4081 taatggctga ttttgccgat ggattccttt gatataaaac atcattttgc cagaagattc
     4141 cgtaaagatt ttgactaatt tatctttttc tttgtaatta cggctagata aaatcaagcc
     4201 tttcgattct gcaagctggc tcatattctc cctcctaaaa aataaatctg gaaaagcaga
     4261 ttctgccgat ccagattatt tatgttacga tcttccgctg tttttctcaa cgtacggatt
     4321 tttatccagt ttatttctat acctaaccta taagaagtcc agaatatcag tactcgtctt
     4381 ttcggtagcc gaagtcttgc agatagactt ttttgtctcg ccagtctttt tggactttga
     4441 cccaaagttc tagaaatact ttgtctccta aaagattttc gatatccttt cttgctttcg
     4501 ttcctacttg cttcagcatt tttccgcctt tgccgataat gattcctttt tggctatctc
     4561 tttcgacgat gatcgtggcc tggatatgga ttttatcatt ctcgtttcgc ttcatcgaat
     4621 caacaacgac tgcgacagaa tgaggcactt cgtctcgtgt taaaaacaag actttctcac
     4681 ggatcaattc agaaacgatg aagtattcag gatgatcagt cacttgatca tctggaaaat
     4741 attgtgggcc ctctggcatt tcactgacta atacatccat taagcgttcg acattgtttc
     4801 cttctgtagc agagatcgga atcacttgtg caaaaggcat ctgcgtcgaa taatcttcga
     4861 tgattcccag caactcgtct ggatggatct tatcgatttt attgataatc agataaactg
     4921 gagaatgcac gttttttaga cgttcaataa taaaatcgtc tccccggccg cgtttttgat
     4981 cagcactgat cataaataag actgcatcca cttctttcaa cgcactgtaa gctgcttcta
     5041 ccataaaatc tcctagacga tgctttggtt tatggattcc tggtgtatcg ataaaaatca
     5101 gctgagcttc tggtgtagta tatactcctt ggattttatt tctagttgtc tgtgccttgt
     5161 cactcataat ggcaattttt tgacccacga ttcggttcaa taatgtggat tttcctacat
     5221 tcgggcggcc gacgattgcg acaaaccctg atttatgttc tggcatatac gttctccttt
     5281 ttaattaaac aacaaatgat atattttagg gacaaataaa atcaacccaa tgatgacagc
     5341 aaaacaagcg gtcactaaaa ctgctccagc tgccatatct ttgatttttt tcccaatcgg
     5401 atgaaaatga aaatccgtaa acatgtccac tacattttca aaaacagtat tgatgatctc
     5461 tactatccaa acgagaaaaa cagctaaaag cagccataac cactctatcc gatcaagctg
     5521 gaaaatcacg cccataaata aagcaaggat accgaacgca acatgtttcc tcatattgcg
     5581 ttcttcctca aaaacggttt tgactccttg gacagcaaat tccacggaca tcaggaaatg
     5641 tttgtttttc tctacgtttt tatcttttga gtccataggc atccaagatc tctttctgta
     5701 aaccaaacat ttctttctca tcttctggtg tcatatggtc atacccgttg atatgcaaga
     5761 atccatgtac cgctaaaaag ccaagttccc gatcatatga atggccgtaa gcttctgctt
     5821 gttcagcagc acgttcaata gagatcatga tgtctcctag tacacgaggc aatggattct
     5881 ctccatcttc aaaaatcact gggatttcgt cttcgccttc ttcttccaga gcaaaactga
     5941 tgacgtctgt cggtgcatct tttccacggt aatcacgatt gattacttgg attgccgcat
     6001 tgtccatgaa agtaacagac atttctgttt cctctggcag ctttaaataa gatgctgcaa
     6061 attgcagcaa gtcatcgatt tctttcattt tctcttctga gacttggttt gtttcatcca
     6121 taaaggtaat atccattata catcgcttct ttctattctt cttgttcttc ctttatcttc
     6181 tcaacttctg ttttcatttt aggatatttg atccgagaat ggaaggtgct acttaaaccg
     6241 ctgatcaatg atttttccac ttttcggatt tctttcaagg tcagtccact atcgtctaat
     6301 tgaccatcgg aaatacgctc ttcgataaga ttatggacaa aactttcgat tttttcgctc
     6361 gtcggatgat ccattgcccg tactgcagcc tcacaactgt cagcaatatt gacgatccct
     6421 gcttcacgac tttgcggttt tggtccagga taacggaatt gctcttctgt tacttccgga
     6481 ttgcgttctt tcgctttgat gtagaaataa cgcattagcg tggttccatg atgctgtttg
     6541 caaatatcga tcaccatttg cggcatatta taatcttcca atattttggc tccatctgtc
     6601 acatgtccaa agataatatg tttactgtct tctggcaata agaaattgtg cgggttttcc
     6661 gctcctgaag gcaagttctc aacgaaaaag ctagcatgtt tgatcttgcc gatatcatga
     6721 taatagcaag ctactcgagt tagcaaagaa cgtccgccga tttctgctac agcgttagca
     6781 cttagattag ctaccatcat actatgatga taagtacctg gtgcttcttc cagcaactgt
     6841 ttcaataaag gatgatttgg attgctgagt tcatttaata caattacact gtcatcattg
     6901 accatcaatt caatatatgg gtgtagaccg atcgtcagca agaatgagaa gagtgcacca
     6961 gtgaacccgc agacgagcat taaccaagtc gttgcatcac caaagctcat tccttgatag
     7021 acgatcaaaa tgacatccat catcacagga aaaataatga cccacatggc agcagataat
     7081 ccttgttctg agatccgttt gcgtctgaca atcgttgcca tgagcccaga gaaaacataa
     7141 gacatcaaga taatcgttag aaagttcgtg ccaatcgaat cataaaaaat gaaaagtgcg
     7201 gatactgctt ggaaaatacc agccatgatc cctgctcttc gattcaagaa aaagcttaaa
     7261 acaagtggtg caaaagcagc gggataaaac agcgggatat aagtagcttg ttccgtttgg
     7321 aataattgga aaaatttcat cagcactatg ctgactgaca tagctgtcac atagaaaagc
     7381 acgtattttg ttctttctcc tgcattttgg aattgaagtg aattataaat cagtacgccg
     7441 atttgaagca aaacagctaa aaccatggca attagcggaa aaatcgaagt tgtttgatta
     7501 gtcagtccta gtaattccaa ttttttcata gctgaagcat cgatttgatt accttcacga
     7561 acaatgattt ctccttggaa gatcatcact ggttgtacag cttctcgtgc ggattgtttc
     7621 aattcttctg tctttttctc attcaatacg tcattcacta cgataccttg atccactaag
     7681 tagcgaatca tttgttgttg tgctggtgtg acattcaaat attggatttg ctcttctgct
     7741 tcttgtttaa aagcttccaa ttcactttca cgaacttgtt tgctcatctg ttcgtcaatg
     7801 agcttcagac tctcatccct cacagtatgg atctcagttt cagtcatttc aaatatcgtc
     7861 gaataaaaat tgttcggtag tttttgataa aacgcaacat tttctgcatt cacattttca
     7921 aaatttttct tcagcgcggc gatccgctct tctgtagtag gctttgggat attttcatct
     7981 tcctttgcct tatcaatctt ttcgtcataa gatttgttga tggcatcatt cgtcttatcg
     8041 atcaattcaa atagttgctt gatccgattg ttctggtcat ctgcaagatc tttttggtaa
     8101 gtgtattctg gagtgactgc ttctgctgcc agttttcttt tttgttcggt ttcttccgtg
     8161 ttctcgatcg ttttgttcgc acggatactt tcttctgcta cttggccttc tctgaaatca
     8221 ttggcctttt gtttgacact actaaatgta acgccgcaga taatgataaa aaacaagaac
     8281 agcaggaaag gcaatgctgc ctttccaagt ttttcttgta gtttctgcag tgttttattc
     8341 aacatgtgct tatcctccta agcaaagctt attcttgatg gagatctgcc ttttcataag
     8401 ctttgatgat ttcggcaact acagggtgcc gaacaacgtc acttgcttca aaattaacaa
     8461 aatcgatttt cttgatttgc tttaatgttc gttctgcatg aacaagacca cttgttgtcc
     8521 ctcgaggcag atcaatctga ctcgtatctc cattgacaat catttttgat tgataaccaa
     8581 gacgtgtcag gaacattttc atctgagcaa ctgtcgtgtt ctgtgcttca tccaaaataa
     8641 cgaatgcatc atccaatgtc cgcccccgca tgtaagctaa aggagcaatt tcgatcacgc
     8701 ctcgttccat caatcggttc gtatgatcta atccaaaaat ttggtataaa gcatcataaa
     8761 caggacgcag ataaggatct actttttctt ttagatcccc gggaagaaag cctagatttt
     8821 cccctgcttc cacagcaggc cgcgtcaaaa tgattttttg cacttcacct tttttcaagg
     8881 cagcgattgc taatactact gcaagaaaag tttttcctgt cccggctggt cctacgccaa
     8941 atacgatatc atgtttggct accgattcaa catatttctt ttggccgctg ttttttaccc
     9001 gtataggttt tccattacgg tctttgacga tctcttcctc atacatctca acaaaataat
     9061 ctaatgtgcc tttttgcccc attttcagtg ccgtcacgac atctggtgta cttacatgga
     9121 ttcctcgttt gatcaattcc tgcaatgtcc gaatgactga ggcagccaag gaaatctggg
     9181 attgttcacc aatgatctga atgatttctc cacgtgtatg gatggttgtt tctgttgttt
     9241 cttcaatcag ttttatatgc ttgtcgtgtg cacctaaaag catgctgaca tcatcagcag
     9301 ccgtcaatct aatctctaaa gaactagact cttccgtcaa aaatagacat cccctttttt
     9361 tcgattcatt ctttcctatt ttaccatact cagaagaaac tatatagcaa ataagaaaag
     9421 gttctaaaag agttgcttaa ccataagtac caagaaacgt taaaaaatag ctgcttccat
     9481 tctctaaagg tctcgtttga ataaaaaaag ctgatcatgt aagctatgca taagatagag
     9541 gacaagtatt ccgtctaact tagcaagtat tctctgggta tttttcttgt ttcaaaaaaa
     9601 gaaaacaaaa tatttgaaat attcttgcat ttcaaaaaaa acaggtgtat tataacgtca
     9661 acaaattaat atacagcaaa gcgtcgagga aaacagtagg ctattcagcg atttattccc
     9721 agtgagtcat gtaagtgaga aatgacaata aatcgaatag acgaacatca tttccgagtc
     9781 aatgtcgaaa acgtagatca ttgtggctgc ctccattatc aggctagcgg ataactttat
     9841 cgaaaagcgt ccgtgaatga agaagctttt ttaagcgaaa aaaggtggta ccgttctaga
     9901 atctagagcc cttttcccaa gtggaaaagg gcttttttta tattttaagg tggtggtttt
     9961 catggaatct gatattagtg gtatttctag tttctgcaac gtttattttg ttactcaaaa
    10021 tgataattat tggaggaaaa atttatggaa gaaacaaaaa cttattcatg gaagcagtta
    10081 ttgatggtca gttcacttat cttcgggatg ttcttcgggt caggtaatct gatctttcct
    10141 gtccacttag gtcaaatggc tggatcaaac tggatcagcg ctggtgtagg atttgccatc
    10201 tcaggcgctc ttttcccatt attagctatc ttagctgtag tagtgacaaa aagtgatggt
    10261 ctgtatgact tagctaaacc tgtcggaaaa tggtatgctg ccttgttctt gatccttgtt
    10321 catttaacga tcggaccatt ctttggtaca ccgcggacag cagcaactgc ttttgaaatg
    10381 gctgctaaac cctttttgcc tgaaaaatat gattcattag gtatgttttt attctctgct
    10441 cttttctttt tacttgctta tttccttact gtacatccaa cacgtttggt caaatatgtc
    10501 ggaaagtatt taaactcctt gtttcttgca ttattagcta tcatttttgt cgtttcttta
    10561 ttccaaccca tggggaatct aaaccaatct gctgcagatt cttatcagac gaacgctttg
    10621 acaaatggcg ttttagaagg atacaacacg gttgatgcag tagctttact tgctttaagt
    10681 gtcacttttg tccatgcagt tcgtgatctt ggttttaaag ataaaaaagt cacagaatta
    10741 acagcaaaag ctggtagcct gagcgttgcg ttagaagtca tgatttattt tggtctggtc
    10801 cttctcggtg cgatgagttt gaataagatg tctttatcag aaaatggcgg aactgcactt
    10861 tctcagatca ctaattttta tttaggtaac ttcggtgcta gcttcttagg ggtaatggtc
    10921 acattaggtg tcttcactac tgctatggga ctcgttgttt cttttgcaca agatttccat
    10981 aaactgttcc ctaaagtcag ttatatgaca tggctgcggt taacgacttt tgtctctttt
    11041 gtcgttgcga atgccggatt ggataatatc atccaatggt ctttaccagt attgatgctt
    11101 ttgtatccat tgtcacttgc gttaatctta ctttccttaa cagcaaaatt cttccaaaag
    11161 agtccatttg tctatcaagt aacgatgttc ttttctgccg ttccagccgt attggatatg
    11221 ttggcaaatt cgcctgcatt agtgagtcag caaactgtcg tagcaagctt actaggattt
    11281 tatcatcatt atgttccttt tgctgcatta ggtcttggct gggtcgtccc aacgttgctt
    11341 ggttacgctg gcagcttgct cttttattat gtatatcgcg tatcaggata taaacaagaa
    11401 gcaagtgagt taccagaaga ataaccaaat caaacacaat ttaacataag agatattaaa
    11461 caggaaaaaa aacagctaga agaaagaata agattctttc ttctagctgt tttttagttc
    11521 ttt
//
LOCUS       DAHHZJ010000093        11348 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.094, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000093 DAHHZJ010000000
VERSION     DAHHZJ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.094"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(57..635)
                     /locus_tag="NBL02_001853"
     CDS             complement(57..635)
                     /locus_tag="NBL02_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCD2572533.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(655..1323)
                     /locus_tag="NBL02_001854"
     CDS             complement(655..1323)
                     /locus_tag="NBL02_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2572534.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(1338..2408)
                     /locus_tag="NBL02_001855"
     CDS             complement(1338..2408)
                     /locus_tag="NBL02_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2572535.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(2579..3943)
                     /locus_tag="NBL02_001856"
     CDS             complement(2579..3943)
                     /locus_tag="NBL02_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCD2572536.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(3940..5499)
                     /locus_tag="NBL02_001857"
     CDS             complement(3940..5499)
                     /locus_tag="NBL02_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD2572537.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(5555..8035)
                     /locus_tag="NBL02_001858"
     CDS             complement(5555..8035)
                     /locus_tag="NBL02_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572538.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(8019..8354)
                     /locus_tag="NBL02_001859"
     CDS             complement(8019..8354)
                     /locus_tag="NBL02_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572539.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(8368..9402)
                     /locus_tag="NBL02_001860"
     CDS             complement(8368..9402)
                     /locus_tag="NBL02_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HCD2572540.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(9492..10172)
                     /locus_tag="NBL02_001861"
     CDS             complement(9492..10172)
                     /locus_tag="NBL02_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HCD2572541.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(10342..11301)
                     /locus_tag="NBL02_001862"
     CDS             complement(10342..11301)
                     /locus_tag="NBL02_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCD2572542.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
BASE COUNT         3229 a         2302 c         1992 g         3825 t
ORIGIN      
        1 cgatcttcgt catatcttgc ttcttcacct aactattttc tccctatata acagctttac
       61 gcttctccta atacaccatt caaatataaa tcgatcaatt gcgccacatt tagcggttct
      121 tccacaggtc ttttcgaccg agtgatgtaa ggtgctaaca ctaagaaaaa atgccttgct
      181 gcgatttctt ttgaaatccc atttcttagc acagattggt ttgctgaaaa gatttcttca
      241 aaaggttcac gataattttt cttccataga aagaaaagat gatctcttgt ttcctttgaa
      301 agattgcttt caagatcgtg aagcatcaga aacaaatcga ccagatgtgt ctcaatcaaa
      361 aactgcgcca tttttgtcaa ggttacttga aatcccgttt cgcttgtttg aacgatttct
      421 ctcaaagctt ttccaacatc ttttaagtgg ttttctaaaa ctgccgtata tagtttttct
      481 ttatcaccga aataatgata gagattgggc tgggtgatcc caactttttg tgcgatttct
      541 cttgttgaag tgccgagata tcccttggac ataaacaagt cagtcgcttc tttcaaaatt
      601 ttttgctgtg tatctttgtt gtatcgatgg accatttttc cacctgcttt ttggttattc
      661 tttttctttc aatacaccat cttccatcat aaatattcta tcgcatttgt caatcagccg
      721 tgtatcgtga gtaaccatga tcgttgcttt atttttgtct tttgattctt ttgccaagat
      781 atcgactact tctcgtgcct tttctgaatc caaactagca gttggttcat ctgctagaat
      841 aatggacgga tcattgtaca atgcacgagc gattgcgacc ctttgccgtt ctccaccaga
      901 tagttcttct ggatacttgt tgatcaactt atccacgcca agctgctcaa ataattcgtt
      961 tgcttctttt tgacgatgtt catgtttaat cttatctact aataataact gcttttttac
     1021 tgtcagaaaa ggaaccagat tggaagcttg caaaataaag ccaatttctt taaaacgtag
     1081 tttaacgcgt tcgctttctt tttgattact gaattcttga tcattgataa atacagagcc
     1141 actgctaggc gtctgcaatc cgcctgccaa agtcaaaaaa gtacttttac ctgaaccaga
     1201 aggaccgatg attgcaacaa attccccttt atcgatactc atggttgttt ctttcaatgc
     1261 tttaactact tggtcgccat cttggaaact tttttctact tctttaaatt ctattgcttt
     1321 catgttcttc ctccttctta tccaattgcc ttcaacggat cgatttttac gattgtccga
     1381 acagaaaata gtgcgcctaa cacagctact actaacatca acaagctaat gacgccaaaa
     1441 aacagccaat tgctttggaa tggaaccgca tcaggtagaa caagggatgt tcccactgtt
     1501 cctaatagac caagaagaat accaacaaat gataaaatga atgtttgtgc cactacagaa
     1561 cgcgcaataa atcctcctgt gatcccttgt gctttcatta caccaaagat attaatcttt
     1621 tgcatcgtca gtacataaat gaaaatcccg atcacaatag ccgcaataac gatcaaaaat
     1681 ccgatcataa atccaaaagt caatacttgc gcattatatc caggtaattc attgatgaat
     1741 ttagaaattt tgatctgttc taaatcttca ggtaaattac tgattttacc tctgacaact
     1801 attgcgttaa tccgtgcatt ttcacttgta tcttcttttt cgaagcggat ttcttggtaa
     1861 gcattgatgg ttgtatagag aactggtgag acattgaatt tggcatgatc cgtaaagcca
     1921 gttagtttaa acgtcttgtc actaccagat aacttaactg tatctcctat ggctagacca
     1981 tattcttctt tcaaactaat atcgcctact gcctcatcat cgttatcaaa agcttttcct
     2041 tcaacaagat tgggcatgat gaattgattt ttatcgatac cgaagaagct aacattgatt
     2101 tttccttctt ctgtactgtc tttactagtc acgactccag gtgtttgggc aagatacgcc
     2161 actttcccac cttcgacttc ttctgctatc ttcttagtaa tcatggacat ccctagattt
     2221 gaatttgcat ctttcgaaag cacaatcgaa tctgcctccc acttgtcgac tgctgttcga
     2281 ttatcttgag ctaagccata agctaaccca gtcaaaaaaa ataccaggta ggcgatcaaa
     2341 aacatcaccc ctgcaactaa cgcataacgt aactttgaat gcataatttc atttaatgct
     2401 aaaaacattt aactcctcct cctgaaataa tcattttata caacaatttt atcacttgat
     2461 aaatttttgg cgaatattat gctcaaaaaa ttcttccgct atttcattat atgaattttt
     2521 tattctcgat tgattaatat ttattatatc cccacacaat aaaagaccgt gatatttctc
     2581 acagtctttt atctactcta ttgctatttg ctctcttagc tagtacttta aaagctaact
     2641 cattgaggta gtgaaaacga gaggtgtatt cagacagaaa tccaaaatcc tcaactgcta
     2701 gctgtggaat cgtagttttg tgtttcacga tatccgcaag caggttcaaa tctgataata
     2761 agaattcttg gcttatcagc tgtccgccaa ttatttctaa tgtatccgga tggtagatca
     2821 gctttgcatt tactgttttg ttttcttcgt cgtattgtgg aaatagatca aattctcctg
     2881 aacacgaacc ggtattttgt tccaagaatg gcgcttcatc ttctgtcagc cctacagttc
     2941 ctaaaaagta gtctataatg actgctgctg ttatcttttg tctgaggtcg taagcggttg
     3001 gctgaccact caaagttagc gctactgctc ttgccatatg tacagctcga gtaactaaag
     3061 gtaaaaagag atgccgttta tttttgtcaa aactgactgg gacgatatct ccaatcgcat
     3121 agatgtccgg atcacttgtc tgcagatatt cattgactat cacagtctca tcctcataca
     3181 aagccacttt ttcttttaac aatgtcgtat taggacgagt ttgggcagaa aaaatgacca
     3241 tatctgcctt tattgtttga gacagaagtt gtacggatac tactttctct tctgattttt
     3301 tccaatcgat caagaattca tttggataaa aatttatttg cttagtttgc agctgatttt
     3361 gaaaactgtt gatcatatcc ttatcgaaat atcttgataa aacattcggc atataatcaa
     3421 ctagatgaat aatgtatcct ttatttctca aagcatctgc taattccaat ccaatgtaac
     3481 cagcaccgaa gatcactact tcttttgctt cttttaaagc ggcaagtact tgcttagatt
     3541 gcactagatt tttataggtg tagacatttt cagcttcaat ttgtttatac aaggtgacag
     3601 gagaggaacc tgtcgctacg attaatttat cataggttat ggtctcgttt tttccttctt
     3661 tggatgcgag gatgagctga tgtttgtctg gatcaagttc agttactctc cattcggtga
     3721 tcagttttgc cccactttct tgaagagtcc gcatgttatg ggcaacttca gctaaactac
     3781 gtatctttcc ttttaaataa aggttgattc catttgaaac atagcctagt ccatctttat
     3841 gatcttcatc gatcagtaaa acttctgctt tcggttgaag ttttcttaga tttagggcag
     3901 ctgtgatgcc ttcatgggaa gcaccaatga tcacgatttt catgttttat tcatccttaa
     3961 attcaatttt tctaaataac attcttttaa aaattggttc aaatagggga aatcataatt
     4021 atattcgttg ctagtgaata caaaaacttt agcggacttc tgtgacgggt actctttcaa
     4081 ttggctagtc aaaatgatat catactgctt ttctttctcg tattcgacta aatcatattt
     4141 gatcgtattc agcaattgtt cctggatgat ttctttcacg atatccgcca ttagcctttc
     4201 ataaggaaga tcataagcaa tttgcagacg taattctaac tgcttgatca gcgtccgtaa
     4261 aatagatgca tagcgagcaa ataattcaaa gcgggcattt tcctcgtcta cttcagtaaa
     4321 atcaacaata gcaaatgttt gtctgaccaa tctttcacat aaaaccagga attgtttatc
     4381 ttctacttca gttaaccgct gttttaatcc ttcttggcca aaaatattca tccatcctct
     4441 aaaataggca aaatgagcgt gattttgcat cactgtcaac gcaatctttt gcgaaatctc
     4501 ttctggaaaa acataaaaag agaaatattc ttctatcgat tgtttaaacg ttcgattcaa
     4561 ttgagctact acgtcgtctt tgtatttttc gcttatcaaa aactcctgga catgcgagtt
     4621 atgtgtatct aatgtaaagt tactgatcat gaaaagataa aaaaccaacg tttcattttg
     4681 attccaagga aaggcatatt gaaagaagta acgggtcaaa aatgtatgga ggtccaataa
     4741 aaattgtgtc gtttgaaaca atccaacagg aagattgcta taatctggtt tggatttttt
     4801 cgtacagcgc attcgcttct tcataatgga aagccatatt tcaaatttca atcgtccttc
     4861 ttctgtaata gcaaaactaa actctttttg cagcagatct aaaaaagtgt ttcctgataa
     4921 atcactgtat tcttttttgt tttcaagata gggacgtcca aaccaaaata attggaaata
     4981 aaaataacga atttgacttt ctggaccggt cagccgcccg ctttttattt tcagatgaaa
     5041 ctcatttaaa gaatcattta attccttcac tctgcgagac aaagtagccg aactgatatt
     5101 aagttccatg acaagtttct gccgatcaaa tgagccattt tccaacagat aaagaagtat
     5161 ctgatatttt gtagaatagc gcaagtaaca atgataataa acatcgactg ctgctatatt
     5221 tccatgtctg attaataaaa tattcccttc ttggataaca atttccaatt gtttgatatt
     5281 ttccaagtta gcttgtaaat aaaacagatc ttctttcaag gtagcccgat caactgataa
     5341 gcgggtaagt acgttattaa cggaaacctc tcctttttcc tctaataaaa gttggaaaag
     5401 tttgatttgc cgttgttcat ttttttcaag caaactgaaa agttccaagt ccactccttc
     5461 aagcatttct gattttaaca gcagaagttt ccttctcatc cttgaaaaag aggatctgtg
     5521 acagttatca cagatccata caaattctaa tcacttagct ggttggccca gctgtcagtt
     5581 cccatgtgac catgccttga tacgttgcgc caggttgagc attcgtactg ctatttacct
     5641 ctaagagtaa accatcggta tcgggaaagt caacgagaac ctctccttta gctgttccat
     5701 cactctcata aatcaacgtg ttttgacctt gctgaatcaa ttgctttgat ccagaatgat
     5761 tgaagtataa ttttgttccg cttaattctt gaccttgatc attcttaaat aaagaagtca
     5821 atgtacctgt cagttgccaa ggagacgtgc ttggaagccg agtatcccgc acatcaaaag
     5881 aaaaatcgcc ttttgattca taataacttg tcttcaacgg tttggggaga tcgttaccaa
     5941 atgtaaaatc atctggtaca ttaactaatg ttaattcttg tttggggaca agaattgttt
     6001 tagcagatgt ttggacaact gggttaggaa gcttagcacc atctgctaat gtaatattgc
     6061 tggaaagtac agctgccttt gtcagtatgc tgacggattg taaagaagta tctacagtat
     6121 ttaattgata gttgtaagta agatgaatgg tatctcctat tttgctcaat agatctgctg
     6181 ggacagaaac tccttgttga atgccggtat ctgcgatagt tgtaccattt aattttaagg
     6241 aaccattgat ataagtagta ttttcaggaa gtgagatcgt gatttttttg tcggttatcg
     6301 catccccaga ttttgttaac gtgaatgtca cattttcttg aatcgtatct cctgatagag
     6361 cagacagggg atctcctgtc tcaggttgtc tgctaaggtt ttttagctga ctatctgcac
     6421 tcacagatgg aaaattagcc actgttattt gaggaagagt ggtgattcca gcttgttcag
     6481 actgccattt gtacgcaaaa gacggataca tgttagtaag tacatcgtcc accaagcgat
     6541 aatcaaaata atattgccct gaagccaatg caggtagtgt agaagtagct gtatttacac
     6601 ccccgttatt tgattggatc gtttggaaaa gttgatctgt ccatcctgtc gctaattttt
     6661 gggttccttt cgaataaagc ctgtaatgga tcgaaccctt ttttagttca gttgctgacc
     6721 aattggccgt gacttggtac gatgcagaag aaaggagctt tccccactga ctatcggctg
     6781 ttaagtttgt tggtaaaacc gttccgtcta gcttgtactg ctccaatacc tttaaagcaa
     6841 caggagaaag caatccgcct tgcgcatctt tcccactgta cacgatattg gctgtctgat
     6901 tcaatccact tgtgcttgct ggagtatgag tcagtattgc tccttgtgta acgttttttc
     6961 ctgtaaaata gttgactacg cgatcaaagc gactaaatgg caactgatca aaacgtcctt
     7021 cagtcagccc catcgtcaat gtccgactat tggtagagcc tggaaggttc tggttatttc
     7081 catccacgta ttctgtggta tcagcaaatg tattatattt tcctgttgaa tcacttggtg
     7141 agatgtaact attccacgta tttatctctg cacttgcaga aaatgcatat gctcctggat
     7201 tcaagtttcc gccagttaat ttaggataaa atctacctgc agctggagct aaaatcgaat
     7261 tgaacatacg atcttggata atattattcg aatcataaaa atcaacagaa aaaacggcct
     7321 ttttagacat agaaggttgt aatttaccct tggtcaaaat tttgtttgga ctcgtatcat
     7381 atttatctcc gttttctgtc atcttcgcga cattagctgt aacatcaaat tgaagcggga
     7441 tatcgtccac gttgaattcg cctcgcgtaa taatagcaat aatatctgtc aatagagtat
     7501 ttgtatccca cgaccccatc ccaaaacgca aggtatggct tcctgctgat aataaacttc
     7561 cgctagtaac cttgatgggg gcaccggcaa tatacaagta ggcgttgttc cagtcaattg
     7621 cttttaatac atcactgctt gatacatgtt ccggcaaatt gatgatcgcc acaaatgcaa
     7681 atccttgtgc agtgatattc accaaatcaa gtacatttgt tagtttcaca tggctttccc
     7741 aatgaaaacg gaccgtgcca cctttccaag gatcatttga accatcgttc aaataagtag
     7801 cagtcaattt aggcaattgg ttagccccag ttagttggac aagcttcccg ccattgactg
     7861 cactacgtcc accatttgga ggtacgatat tttcatacgt gtatggatca taagaaatgg
     7921 caggaggcgt tgctccaaca gcttctgccg aaggtgaaaa gataaatata cttaaaacaa
     7981 aaagcgaagt aaaaaagaag gagcgaaaaa atgaaaattt attttgtagc aacatgttca
     8041 tcctcctttt cctttcttct ccataacaac aaccacgcaa gaagccataa ttctatacca
     8101 atgatcaaat aaagaaaacg gatcttttgt tcattcgtct ccgggaatac gccatgttga
     8161 ttagattctt tataaatcgg tagctgtgtc tgctgttttg atgcgccatt gactgaacca
     8221 cttggatgcg gaattggact tagatcataa aatgtaatcg tacctgaact tttggtatct
     8281 gcttggactg gctcttgcca gctgaagaac agcaaacttg caataatccc tcccaataag
     8341 atcgatgttt tcaacgaaat cacctcatca tgttgtttct ccttttttct ttttcttgtg
     8401 gacccacaaa aacagcaaaa tcaaccataa cactagaact gcagcaacca atcctgcata
     8461 aagccatggg aaagattgtt cttgtactgc aaaaacggaa ttttcattta ctttttgtgc
     8521 tttttctttt ttgatcgtaa agttttgtgt aaatcgccat tcattactgt cgcctgattt
     8581 tgctatgatt tctgcgcggt aatcgccggc ttttgcttta tcaccagcta aatcaagatt
     8641 caagtgaaaa ttcgaatttg gtgcaactaa aaatgcatta gacgtatttt caatataaag
     8701 ctgattttca taaaagatct tgctcgtaaa tatcaaattg ctgataatcc taggctgagg
     8761 attttggaaa cgaagctgaa cagttggctt tgcgttgacc tcgtccaaat ctcctgataa
     8821 aagtttcaga tccggttcta tcgtctcttg actttcttgt aaaacgactg cgagattata
     8881 agcaatacga tttttgattt gtgctccttc tgtagcattt tctgtttcag cttcttcttt
     8941 aggtgaaaca tgaatacccg ctaatacacg tcccttaaag ctttttgctg ggattttcac
     9001 ttctataggt acagttgttt ctgcatgtgc tgatacattg atttcctttt tcgcaatgag
     9061 caaaacgtca cggacatcaa acggcaatgt ctcatctaac gttgggtttt tttgactata
     9121 atcgatcgtt ccaccatcac tcgttaccgc cggattgaca gagacctcaa atgttcgttc
     9181 ttcatctgaa gaattgctga taattacttc tagtgtctga ttttgttccg gtgccactaa
     9241 taaatcaaaa taaccgattg ctttatctat ttggttctct ggaaataccg gtttgattcc
     9301 aaagtctcct gctgaatcct ttgcttggct gattgacgga aaaatgaaaa atagacttaa
     9361 aatagaaaaa aacgaaacaa acagtcgttt actttgattc attgtccacc acatcctttc
     9421 aacaacaact gcggtaaaaa atccttcacc tatagtattt tcaccgcagt ttgttgaatt
     9481 tatttcatac tctatgctac tgggccagcc acgaggttcc aagtcaaatc agccgtgaat
     9541 gttccagctg ctgctttcgg cactttcagt gtgatatttg ctggatcata aacagaaagc
     9601 catgttccca gaccttgatc ttttgctgct gtaaatattg gtgtcgctgc acttgcttta
     9661 ctcaacccag ttacagtttg actagttggt ccagtggatt cgttatctga cgtactttta
     9721 acagtggatg ttccaaaatc taattcagcg ccttgcaaaa cttgtgaacc gtttacaaaa
     9781 tctgaaaccg aaactgtcac gttccaccct tgtgcttggc catctgcacc tacacctcga
     9841 cgatctgata cttggagata aggtgtatcg ttttttgaag cgtctacttg atacgttttt
     9901 gttccgcttt caatctcgtg tgtaccaaat ggcaattcgg gaactacatc cagtgttaaa
     9961 ggacctgtct ggccagttgc tggattttcc ggatctgctg gctctactgg ttttgaagga
    10021 tcagtagggt taagaggctc cacggtattt gttggttcag taaaggtaat atctcctttt
    10081 gaagtcgctt gaccctcagc aaaagctggt gttgccaagc cgcctagaag aagtgttgaa
    10141 agtaataatg tcccaatcat gttctttttc ataataaatc actccttttt cttagtgacc
    10201 taagtatagt agagacagta tgcaaaaaat cctcatgaat tcatccgctt acttgaaatt
    10261 atgacccttt ttgtataaaa gagaaaaaaa caataaaatc gtttacatga atattcttcc
    10321 ttttttatta agtttcgttt gtcaaattaa gttagacaaa tcatcttttg tgacgtaact
    10381 caaaaatact tccattggtg tccgatagtg taatgatttt ctaggaatat gatttcgttt
    10441 cgatgccacc gattggataa aggtttcaga tacaccatta aaatccatag ctttatacaa
    10501 accatcttta cgtaataagc catttgaatg ttcattcaat cctcgttgag aaggtgttcc
    10561 tggatccgca aaataaattg aaatatcgtt ggtattacta atctttttcc aatttgagaa
    10621 ttcttttcca caatcaaatg tgatggattt aaataagttt tttgggacac aacgaaacca
    10681 ttgattcagc ctattttcaa tgtctttagc ctgtctgcct tcgggtttca aggtaatgat
    10741 gactttagat agccgttcaa ctaatgtgat gaccgcgctt ttatgttttc cacccacgat
    10801 agtgtcacct tccaggtgac caaattcatg atcaaactgg gtatagtctg tctttctgtc
    10861 tcggattgac cggcgaaacc cttgttttcc tcttttttct ttatgaccgt ttggttttct
    10921 ttttcccttc attggtaaat cgtgagtgtc aaaggtttta tctttaaaca ttcgatacag
    10981 tgtgcgtacc gaacaagcaa taggaaactc tgctcgacca ataaggacat caggagccca
    11041 cccttgaaca accttttttt gaatgtattc ctgttgctca tcaggtaaaa caatcggatg
    11101 tcttccgcag ttacgtttgt ttttcttata ttgtttatag tagctcattg cgcttccacc
    11161 agatttaaga aaacgataga ccttataaat agtttgtcga gaacgttgta acagattggc
    11221 tacttttgaa acaggttgtg attgattgaa ataagcttct atcattacga gttcagttgg
    11281 tgtaagatgg ttataggtca tttgtactca ctccttataa ttctttcgtc ggaactattt
    11341 tgagtgta
//
LOCUS       DAHHZJ010000094        11326 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.095, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000094 DAHHZJ010000000
VERSION     DAHHZJ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.095"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            71..256
                     /locus_tag="NBL02_001863"
     CDS             71..256
                     /locus_tag="NBL02_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD2572543.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            269..517
                     /locus_tag="NBL02_001864"
     CDS             269..517
                     /locus_tag="NBL02_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572544.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            548..718
                     /locus_tag="NBL02_001865"
     CDS             548..718
                     /locus_tag="NBL02_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572545.1"
                     /translation="MGKFNRALVFSAPLIVYALGLWGSRQALIGTIVYMVWIFIGLDE
                     AEYKTKKPVGRD"
     gene            complement(820..1278)
                     /locus_tag="NBL02_001866"
     CDS             complement(820..1278)
                     /locus_tag="NBL02_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572546.1"
                     /translation="MIKILSDYQVAITLIISISGFFLSLYNLFKDKRKITLSYFLVKH
                     DRSNRMILTGVIANPSKMPNSIIECTYLYNGKKINCSHYHANGFDMGSIHKSSLLSPI
                     PLAQAISPGYSVAFSEVLDMKDILPGEKLVMVIRTANYQKKFKLKLKDSF"
     gene            1446..1763
                     /locus_tag="NBL02_001867"
     CDS             1446..1763
                     /locus_tag="NBL02_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572547.1"
                     /translation="MLIATDTLDRIFLKDEYKLRNIDASGILVFDLYDNGKIGIYQAS
                     DIEETNLAFEQIDDSVELDLDEAILAFEQIAKLLKEAQKNGNSLPTQRVIYQSPGTGR
                     TIG"
     gene            1696..2175
                     /locus_tag="NBL02_001868"
     CDS             1696..2175
                     /locus_tag="NBL02_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HCD2572548.1"
                     /translation="MATLYQLSESYIKVLELAEQLDEEILRDTLDSINEAIEYKAENL
                     AKIVKEVEGKAELIDSEIKRLQERKTSLLNNAKSIKHYLQEEMEKTGKTKIKGELFNI
                     GIQNNPVSVNVINENLIPKGFFTPVPPKLDKKQLKEELKHGDIPGAELVQTKGLRIR"
     gene            2192..2368
                     /locus_tag="NBL02_001869"
     CDS             2192..2368
                     /locus_tag="NBL02_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572549.1"
                     /translation="MEHENVKDALKAAIEIAEAKGIKVDGKPATVHDIQNLTKEHLYF
                     IADLLGLSELYLDK"
     gene            2625..3056
                     /locus_tag="NBL02_001870"
     CDS             2625..3056
                     /locus_tag="NBL02_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572550.1"
                     /translation="MKNKLAKTTAIIGLALGSGVIGYAASNAFQDLDTIKANFNTVLQ
                     YGQTKSQRVSELESQLSNNTRTQEQLKAEIEQIKSDKQKEIEAKQREVEQKQQEIATK
                     QQEADSLRQQLSTVQNDKEQLEQRVSELRQYTDQKVGELGK"
     gene            3062..3379
                     /locus_tag="NBL02_001871"
     CDS             3062..3379
                     /locus_tag="NBL02_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HCD2572551.1"
                     /translation="MTIYDPNISWAKHTIKVSFMIWGYKGYVTYKVGGNTKGLSLIAI
                     DSDDLYDANFEDNPVNFRDLDEDWFSMELTNDKGDSTLVEDEFDRLGDYIVGVEIIAH
                     EPE"
     gene            3498..4184
                     /locus_tag="NBL02_001872"
     CDS             3498..4184
                     /locus_tag="NBL02_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCD2572552.1"
                     /translation="MQIKKATDIEQTKGTYLIYGAPGKGKTSTAKYFPGKTLILDIDR
                     TSKVLRGEKNVDIVYIDNEDTWNDWGNTLADLTTNYVGVYDNVVVDNVSELERCILSS
                     LGAEGKNNGVPSQGDYQYMQFRMVNSLRYMKNLDSNLIWTAWEEIDLWTDSDGSSYNI
                     ALPQINRKIRNNILGLCDVVGRLMVKEDDERGFILQATDSTYAKNQLDNRSGCKQSEL
                     ILDGIVRLPE"
     gene            4159..5508
                     /locus_tag="NBL02_001873"
     CDS             4159..5508
                     /locus_tag="NBL02_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCD2572553.1"
                     /translation="MELYDYQNDLVDRARQAYVDGYKAPCIVSPCGSGKSVMVAEIAK
                     RTTRKGNRVLFLVHRRELIDQIKDTFQKMGVDNQLVNFGMVQTVVRHLKTIKKPQLII
                     TDENHHGLAASYRKIYEYYSDVPRLGFTATPIRLNGSGLGDVNDLLIEGVSAKWLIEN
                     HRLAPYEYYAPKLIDTAELKKASTGDFTKKSMDKAVKNTIYGDVLKHYKTLAEGEQAI
                     AYCHSIDASKHTAEIFNDAGYKATHIDAKTPKDERADIIEAFRNHEIKILCNVDLIGE
                     GFDVPDCSTVIMLRPTQSLSLYIQQSMRGMRYRPDKTSIIIDHVGNVNRFGLPDMDRE
                     WSLDTKKKTKTDSDLSIVQCAFCFGAYERPKGDNICPYCGELQPIEERKSELEIDESA
                     ELMKVGETKITLNFENNKYYNMTEDEAESIEDLYAIARAKGFKPGWAYMAAKRKGWL"
     gene            5499..5972
                     /locus_tag="NBL02_001874"
     CDS             5499..5972
                     /locus_tag="NBL02_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCD2572554.1"
                     /translation="MVVGMGGIFGKKAEDVQGSIINGVTIIGDTGKRDSSGNQIVIAR
                     NKNNEVFESSLSNIKRGVVNGTGEESGYVNLKKANEKYHVEGTYVKALSVGGTKGVNW
                     DKRRNKWKARLYFKGEYVLQKDFNTKEEAIQARLAAEEKYFKPILEKYENKKEND"
     gene            5947..6474
                     /locus_tag="NBL02_001875"
     CDS             5947..6474
                     /locus_tag="NBL02_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HCD2572555.1"
                     /translation="MKIKRRMINMTGFNLDFSKAQQGNEIKDGTYEVVVNKAVENATK
                     SGAEFIDIDLIVRNDVDQPFQNKHIFAKIWKAKATGKYNEGMIMAIAQALQLEDGKSY
                     NGFDELLADFVLKTASVRVKTEESNGYKNVNVKSWDKTNTRGVMNHQFKNGDEPSFGP
                     ERSRATTVRNDNLPF"
     gene            6489..8801
                     /locus_tag="NBL02_001876"
     CDS             6489..8801
                     /locus_tag="NBL02_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCD2572556.1"
                     /translation="MYEKIPKELKNLKQWCVYKLVWDEKRQKHTKIPYNANNGHKAKS
                     NDESTWSDFQTALAAIDHYGMSGLGFFFKPPYFGIDIDNAEGEVERFKSGDIEENIIY
                     EFIESMRSYAEYSQSGTGIHIIARGELPGGRRRKGDVEMYQNGRFFVMTGNAASKYLE
                     ITEPNPKDIKRLYDRYVGDEKIIQFKEENPLMNTVDLPVEEIIKRAESSSQGARFKVF
                     MNGGWEAFYSSQSEADMAFANDLAFWTGRDFEKMDEIFRASAMIRSKYDQKRGSATYG
                     ESLLNKAIADTNSVYNPKRKSDFKIFIKDQEQPKEEKYYSYDDTGNADRFTDIYGTLV
                     KYSYIDKSWYYYDGKVWLQDNTGEVRKMIDTTVDIMGKEPLTIPEGADDETKEALMKA
                     KEKHVKRSRSNAGKNAMMDELKHRLSVLPEEFDKDKTLFNTQSGYLSLHDGLLHEHEI
                     DKMFTRVSNVEYTESVDCPMWEEFINQIFDNDQELIRYIQKCVGYSLTGSTREQCMFI
                     LYGHGSNGKSVFLEIISELMGNYAMTMQAQTIMVKQSQSSANSDIARLKGARLVTSSE
                     PNEGVRLDEGLVKQLTGGDKVTARHLYGKEFEFEPEFKLWLATNHKPIIRGTDDGIWR
                     RLNLIPFTVQIPDHKKDKNLKFKLQTELQGILKWAIDGCLLWQREGLEKPKSVVAASQ
                     DYRNEMDQIGTFIETCCETGPGLKISGGELYKVYREWASDNGEHTFTNTKFGREISKK
                     YSKEKAGGFMVYKGITLKPRKYDNVRELFK"
     gene            9048..9365
                     /locus_tag="NBL02_001877"
     CDS             9048..9365
                     /locus_tag="NBL02_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HCD2572557.1"
                     /translation="MKSEHQIQDEIRIALSKHGCTIFRSNAGQVTTIEGRQFYGMPKG
                     FPDLCGHRNSDGKAIYIEVKNERGKLREDQKRFAEFLNGQPVLYGVARSVEEALKIVE
                     ESS"
     gene            9362..9523
                     /locus_tag="NBL02_001878"
     CDS             9362..9523
                     /locus_tag="NBL02_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCD2572558.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            9526..9825
                     /locus_tag="NBL02_001879"
     CDS             9526..9825
                     /locus_tag="NBL02_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572559.1"
                     /translation="MDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWSEKTKRR
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRAKRENVDSKGRQFKHWEKK"
     gene            9825..10139
                     /locus_tag="NBL02_001880"
     CDS             9825..10139
                     /locus_tag="NBL02_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HCD2572560.1"
                     /translation="MEELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EYGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEDS
                     DE"
     gene            10132..10662
                     /locus_tag="NBL02_001881"
     CDS             10132..10662
                     /locus_tag="NBL02_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HCD2572561.1"
                     /translation="MNKKVLIDKQALIDELMKIPGVGSNSDALETIKRFPSYEPQKPV
                     VPKFVAELLDYYRQSTDVDLLALLITFHDWYYRKTKDGEHEEAIDWLVDHPEIYMRAW
                     LFGYELEKEPLYCVIINGRYLVKVFSNTNVVILVPADEFSQYVTQKFQLTEKQIKAID
                     ERYWPFAVPVDEVVEG"
     gene            10659..10928
                     /locus_tag="NBL02_001882"
     CDS             10659..10928
                     /locus_tag="NBL02_001882"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572562.1"
                     /translation="MSDPYITKLEKLSEDFEKNKNKLTKDKLIELVRLVIRGAITREK
                     AQQNIEIFARSVRDGLEKKVKQNEQQTPQNNKTKKTGTECTKDKV"
     gene            10858..11061
                     /locus_tag="NBL02_001883"
     CDS             10858..11061
                     /locus_tag="NBL02_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HCD2572563.1"
                     /translation="MNNRHRRITKLRKRELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASGAIRKFGISILSDDRKWEEKE"
     gene            11058..>11326
                     /locus_tag="NBL02_001884"
     CDS             11058..>11326
                     /locus_tag="NBL02_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572564.1"
                     /translation="MKLKDGFYSSSHGIGGLMLDMPTKNPKTREKPKFKVGDMVRCEA
                     EGFIYPFCGYVEHLYNHSAIIRIENTMECDKWLAKSKENLAVARLV"
BASE COUNT         4058 a         1828 c         2520 g         2920 t
ORIGIN      
        1 gctaataggt gtcaacgatt tagctaattt tttagcttac aaattatttg tttagaaagg
       61 agctattttt atgtctgaga atttagactt aaaaattcga gcggagatga gaaaaagaag
      121 aatgactttc aaagaactag ctgcgcttat cggtatttca ggagcttatt tatcagatat
      181 tctaaacggc aatcgtgatg gaaagaaagc acaacagcat atcgaaacag tgaaaaaaat
      241 attggacatc cgataggggg ggtaggcgat gacaaaacta aaaaaacaag attttgtaaa
      301 aaaatataat tattctccat ctacttatca acgtcgaatg tcggaactaa aaaatacagc
      361 aattttctca gcggcgtatg aacgggtcac aggacaagaa gtttggatca atacagaatt
      421 atacgataaa tttttgtctt tcaaatccta taacaggtta cgcacaagaa aggtaacacc
      481 taaagaattt atcgagaagc atttagttga tttataaaaa acagaacatt ttgagaggtg
      541 aaggttaatg ggtaaattca acagagcatt agtattcagc gcaccgctaa tcgtctatgc
      601 tttagggctt tggggaagta gacaagcgtt gataggaacg attgtgtaca tggtttggat
      661 ttttataggg ctggatgaag ctgagtacaa aacaaaaaag ccagtcggga gggactgact
      721 aatgagtaag gacgatattg tatttctgtt aattatgttt tgtgggattg gatcaataat
      781 aattaatttg tatttgatta ttaaagaaaa atttttcaat taaaatgagt cttttagctt
      841 taacttaaat tttttttgat aattagctgt tcttatgacc attacaagtt tttcaccagg
      901 taatatatct ttcatatcta aaacttctga aaaagcgact gagtagccag gagagattgc
      961 ttgtgccaac ggtataggag ataaaaggct acttttatga atgcttccca tatcaaaacc
     1021 attggcgtga taatgtgaac aattaatttt cttaccgttg taaagatacg tacattcgat
     1081 aattgagttt ggcatttttg atgggtttgc aattacgcca gtaagaatca ttcgattact
     1141 tctatcatgc ttaactaaaa aataggagag cgtaattttt cttttatctt taaataagtt
     1201 atagagggat aaaaaaaatc ctgaaataga aatgattaaa gtaatagcga cttggtaatc
     1261 gctcaaaatt tttatcaagt aattcaccac ctttttactc aaattatatc aaaaaggaga
     1321 gaagaaataa tgcaagaatt agtaattttg aaaaataaaa aaagacacga acgccggcaa
     1381 gcatgatcgt gtcagggaaa taaactatct ttctatattt taccacagaa aggaatgaac
     1441 cgtaaatgct aattgcaacg gatacactag accgcatctt tttaaaagac gaatacaaac
     1501 tgcgcaatat agatgcgtca ggaattttag tatttgatct ttatgacaat ggaaaaattg
     1561 gtatctatca agcaagtgat attgaagaaa caaaccttgc tttcgaacaa attgatgatt
     1621 ctgtggaatt ggatttagat gaggcaatcc tagcttttga acaaattgca aaattattaa
     1681 aggaggcaca aaagaatggc aactctttac caactcagcg agtcatatat caaagtcctg
     1741 gaactggcag aacaattgga tgaagaaatt cttcgcgata ctctcgattc gattaatgaa
     1801 gcgatcgagt ataaggcaga aaacttagca aaaatagtta aagaagtaga agggaaagct
     1861 gagttaatcg atagtgaaat taaacgtctg caggaacgta agacatcgct tttgaacaat
     1921 gctaagagta tcaagcacta tttacaagag gaaatggaaa agactggtaa aacgaagatt
     1981 aaaggtgaat tattcaacat tggaattcaa aataatccag tatcggtcaa tgtaatcaat
     2041 gagaacttaa ttccaaaagg attctttacc cctgtgcctc ccaaattgga taaaaagcaa
     2101 ttgaaggagg aactgaagca cggagatatt cctggtgctg aactcgtcca aacaaaaggt
     2161 ttgaggataa gatgatgaag gaggagaaat aatggaacac gaaaacgtaa aagacgcttt
     2221 gaaagctgct atcgaaatcg cggaagctaa aggaataaag gttgacggca aaccagcaac
     2281 tgtccatgac attcaaaatt tgacgaaaga acatctttat tttatcgccg acttgcttgg
     2341 tttgtcggaa ttgtatttag acaaataacc ccacacggaa ggagtggcta gggtggttgt
     2401 attacaagta atagtctgcg taacattcat ttggtcgtgc ataccggttt gtatatatat
     2461 catgaagaag cttcgccctg agtcttacaa attatacgca atttacagca taacagtagc
     2521 tcttgcattt acattgtttg caatttctag gcttccgttt taatcgtggg aatgaatttt
     2581 taatgaacta aattatctat aaagcaaaaa aaggagaaac aacattgaaa aacaaactag
     2641 caaaaacaac agcaatcatc ggactagcac taggtagcgg cgttatcggc tacgcagcaa
     2701 gcaatgcgtt tcaagatttg gacacgatca aagcaaattt caacacagtc ctacaatacg
     2761 gtcaaacgaa atcgcaacgt gtgtcagaac ttgaatcaca gctatccaac aacactcgca
     2821 cacaggagca gctgaaagcc gaaattgagc aaatcaaatc ggacaagcag aaggaaattg
     2881 aagccaagca acgagaagtc gaacaaaagc aacaggagat cgctacaaag caacaggaag
     2941 ccgatagctt gcgccaacaa ctatccacag tgcaaaacga caaggagcag ttagaacagc
     3001 gcgtgagcga gttacggcag tatacggatc aaaaggtagg ggagttgggg aaatgaaaga
     3061 gttgactatt tatgacccaa atatttcatg ggctaaacac acaattaaag tttcttttat
     3121 gatttggggt tacaaaggat atgttactta caaagtgggc ggtaacacta agggtttatc
     3181 attaatagct attgattcag atgatctata tgatgctaat ttcgaggata acccagttaa
     3241 tttccgtgac cttgatgaag attggttctc tatggaactt actaacgata aaggcgacag
     3301 tacacttgta gaagatgaat tcgacagact aggtgattat attgtcggtg ttgaaattat
     3361 tgcgcacgaa ccagagtagg agagtgaata caaaatgatt aaaagaatca ctatatcagt
     3421 tgaagaagat agtggatcaa caatgcagca taccgacaat cgaaaaactt ttttaaccaa
     3481 tagaaaggag aattttgatg cagattaaga aggcgacgga tatcgaacaa accaagggaa
     3541 catatttgat ctacggtgct ccaggaaaag gtaaaacttc aacggctaaa tattttccag
     3601 ggaaaacact gatccttgat attgatcgga cttcaaaagt ccttagaggt gagaaaaatg
     3661 tcgatatcgt atatatcgac aacgaagata cctggaacga ctggggcaac acgttggctg
     3721 atttaactac caattatgta ggtgtttatg acaacgtggt tgtcgataat gtcagcgaat
     3781 tggaacggtg tattctgtcc agtttaggcg cagaaggaaa aaacaacggc gttccttcgc
     3841 aaggcgatta tcaatatatg caatttagaa tggtgaacag cctacgctat atgaaaaatt
     3901 tagacagcaa ccttatctgg accgcatggg aagaaatcga tttgtggacg gattcggatg
     3961 gatcttctta caacatcgct ttgccgcaga tcaatcgaaa gattcgaaac aatattttgg
     4021 gattatgtga cgttgttggt cgattgatgg taaaggaaga cgatgaacgc ggatttattt
     4081 tacaagccac agattcaacc tatgccaaaa atcaattgga taatagatct ggctgtaagc
     4141 aaagtgagct gattttagat ggaattgtac gactaccaga atgatttagt cgatcgtgca
     4201 agacaagcct atgttgatgg ttacaaggca ccatgtatcg tatcaccgtg cggttctgga
     4261 aaatcagtca tggttgcaga gatcgctaaa cgaaccacac gaaaaggcaa cagagtgttg
     4321 ttcttggtcc atcgtcgtga attgattgat cagatcaaag atacgtttca aaagatgggc
     4381 gtagataatc agcttgtaaa tttcggcatg gttcaaacag tcgtacgaca tcttaaaacc
     4441 atcaagaaac cacaactgat catcactgac gaaaaccatc acgggttagc agcgtcctac
     4501 agaaaaatct atgagtatta ttcggacgtt ccgcgattag gttttaccgc gacacccatc
     4561 cggttaaacg gtagtgggtt gggggatgtg aacgacttgc taattgaagg agtatcggct
     4621 aaatggttga ttgagaatca tagattagct ccttatgaat attatgcacc taaattgatc
     4681 gatacagccg aattaaagaa agcttcgact ggagatttta caaagaaatc aatggataag
     4741 gcagtgaaga atactattta tggcgacgtg ttgaaacatt ataaaacgtt agcagaaggt
     4801 gaacaggcaa tagcatactg tcacagtatc gatgcaagta aacacactgc tgaaatattc
     4861 aacgatgctg gttataaagc aacacatatc gatgcaaaaa ctccaaaaga tgaacgtgca
     4921 gatattatcg aagcgttcag aaatcatgaa attaaaatcc tatgcaatgt tgatttgatc
     4981 ggagaagggt ttgatgttcc tgattgttcg actgtaatta tgttgagacc tactcaatcg
     5041 ttatcattgt acattcagca atcgatgagg gggatgcgtt accgaccaga taaaacgtca
     5101 atcatcattg accatgtggg taatgtgaac cgttttggat tgcctgatat ggatcgtgaa
     5161 tggagtttag atacaaaaaa gaaaacaaaa actgatagtg atttatctat cgttcaatgc
     5221 gcgttttgtt ttggtgctta cgaacggcca aaaggcgata atatttgtcc gtattgcgga
     5281 gagttgcagc cgattgaaga acgaaagtca gagttagaaa tcgatgaatc ggctgaatta
     5341 atgaaagtcg gagaaacgaa aattactttg aattttgaga acaataaata ttacaacatg
     5401 acggaagatg aagcagaaag tatagaggac ttatatgcca tagcaagagc taaaggattt
     5461 aaacctggtt gggcttatat ggctgctaag aggaaggggt ggttgtagga atgggcggta
     5521 tttttggtaa aaaagcagaa gatgttcaag gttcaataat aaatggcgta acaataatag
     5581 gtgatactgg aaaaagagat agttcaggca atcaaattgt aatcgctaga aataaaaata
     5641 acgaagtttt tgaatcttct ctatctaata taaaaagagg tgttgttaac gggacaggcg
     5701 aagagagtgg ctacgttaat ttaaaaaaag ctaatgaaaa ataccatgtt gaaggaactt
     5761 acgttaaggc tttgtctgtc ggtggaacaa aaggcgttaa ttgggataaa cgaagaaata
     5821 aatggaaagc acgattatat ttcaaaggag aatatgtctt acaaaaggat ttcaacacca
     5881 aagaagaagc aatccaagca cgacttgcag cagaagaaaa atatttcaaa cctatcttag
     5941 aaaaatatga aaataaaaag gagaatgatt aacatgacag gatttaactt agacttttca
     6001 aaagcgcaac aagggaacga aatcaaagac ggtacgtacg aagtggtcgt aaacaaagct
     6061 gtagaaaatg caactaaatc aggcgcagaa tttatcgata tcgatttgat cgttcgtaac
     6121 gatgtggatc aaccgttcca aaataaacac attttcgcaa aaatctggaa agccaaagct
     6181 accggcaaat ataacgaagg tatgatcatg gcaattgcac aagcgctaca gttggaagac
     6241 ggaaaatcat ataacgggtt tgatgaattg ctggcggact ttgttttgaa aaccgcctca
     6301 gtacgcgtga aaactgaaga gtcaaatgga tataaaaacg tcaatgtgaa gtcttgggat
     6361 aaaacgaaca cacggggcgt aatgaatcac caattcaaaa atggtgatga accatccttc
     6421 ggaccggaac gttcaagagc gacaacggtt agaaatgaca atcttccatt ttaattgagg
     6481 tgtaatcaat gtatgaaaaa attccaaaag agcttaaaaa tctcaaacaa tggtgtgttt
     6541 ataagctagt ttgggatgaa aagcggcaga agcacacgaa gatcccttat aacgccaaca
     6601 acgggcataa agccaaatcc aatgacgaga gtacctggtc tgattttcag accgctctcg
     6661 ctgctattga tcactacgga atgagtggac tggggttctt tttcaaacca ccttatttcg
     6721 gcattgatat cgataatgct gaaggtgaag ttgagcgatt taaatcaggt gatatagaag
     6781 aaaatattat ctatgaattt atcgaaagca tgagatccta tgctgagtat tctcaatcag
     6841 gtactggcat ccacattatt gctcgcggag aattgcccgg cggaagacgc aggaaaggtg
     6901 acgtagaaat gtaccagaac ggccgattct tcgttatgac tggaaatgcc gcatcaaaat
     6961 atttagaaat aactgagccg aatccaaagg atatcaaacg cctatatgat cgctacgttg
     7021 gagatgaaaa aatcatccaa ttcaaagaag aaaatccgct gatgaatacc gttgatttac
     7081 cggttgaaga aattatcaaa cgtgctgaat cgtcttccca aggggcaaga ttcaaagtct
     7141 ttatgaacgg cggttgggaa gctttttatt cctcgcaatc cgaagcggat atggcgttcg
     7201 ccaacgactt ggctttttgg accggtagag actttgaaaa aatggatgag attttcagag
     7261 cgtctgcaat gatccgtagc aaatatgacc aaaaaagggg atcggcaact tacggagaat
     7321 ctctactaaa taaggcaatt gctgatacaa attccgtcta caaccccaaa cggaaatctg
     7381 attttaagat ttttatcaaa gaccaggaac agccaaaaga ggaaaaatac tattcgtacg
     7441 acgacaccgg caacgcggat cggtttactg atatctacgg aacgttagtc aaatattcgt
     7501 acattgataa atcttggtat tactacgatg ggaaagtttg gctacaggac aataccggcg
     7561 aagttcggaa aatgatcgat acgacagtag acatcatggg aaaagaaccg ttaacgatcc
     7621 ctgaaggcgc agacgatgaa accaaagaag ctttgatgaa agcgaaagaa aaacacgtca
     7681 agcgttctcg cagcaatgcc ggaaaaaacg ctatgatgga cgaattgaaa catcgactat
     7741 ccgtattgcc ggaagagttc gacaaagata aaacgctgtt caacacacaa agcggctatc
     7801 tgtctttgca tgacggtctt ctacacgagc atgagataga caaaatgttt acacgtgttt
     7861 caaatgtcga gtacacagaa tcggtcgatt gtcctatgtg ggaagaattt attaatcaga
     7921 tttttgacaa tgatcaagaa ttaatcagat atatccagaa atgtgtcgga tattccctga
     7981 ccggatctac cagagagcaa tgtatgttca tcctctacgg tcatgggtca aatggaaagt
     8041 cggtattttt ggaaattatt tctgaattga tgggaaacta tgcgatgact atgcaggcac
     8101 aaacgatcat ggtcaagcaa agccaatcat cagcaaacag tgatattgca cgattgaaag
     8161 gtgcgcggtt ggtcacttcg tcagaaccga atgaaggcgt ccgactggac gaaggtctgg
     8221 tcaaacaatt gactggtggt gataaagtca cggctcggca cctttatgga aaagaattcg
     8281 agttcgaacc tgaattcaag ttgtggctgg caacgaacca caagcccatc atccgtggga
     8341 ccgatgacgg catctggcga cggttaaatt taattccatt cactgtccaa atcccggatc
     8401 acaaaaagga taaaaatctg aaatttaaat tgcagacgga gttgcaaggg atccttaaat
     8461 gggcaatcga tggttgcctg ttgtggcaga gagagggctt ggagaaacca aagtcggttg
     8521 ttgcagcgag tcaggattat cgaaacgaaa tggatcaaat aggtacattt atcgaaacgt
     8581 gttgcgaaac aggtccagga ctaaaaatat caggcggaga attatacaaa gtttatcgtg
     8641 aatgggcatc agataatggc gagcatactt tcactaacac gaagtttggc agagagattt
     8701 caaagaaata tagcaaagaa aaagctggtg gatttatggt ttataaagga atcacattaa
     8761 aaccaagaaa atacgataac gttagagaac tttttaaata aatctggatg gttctggagg
     8821 gttgagaacg aaaccctcca acccttagaa acgttgatat atcagtattt atattatatt
     8881 tttctttctt tctggagggt tttaaaaaaa taataaagta ttaaagtaaa aaataaaaag
     8941 taaattatat aaaaaagaat aggtttttgg gtctgaccct ccaaactctc caccaaaatc
     9001 ttcctaaatg ctttgatacc aacgattaaa cagtctggag ggttacattg aaatcagaac
     9061 atcaaataca agacgagatc agaattgcct tatcaaaaca tggttgcacg atttttcgat
     9121 ccaatgcggg acaagtgaca acgatcgaag ggcggcaatt ttacgggatg ccaaaagggt
     9181 tcccagatct atgtggacat cgtaattctg atgggaaagc aatttatata gaagtaaaaa
     9241 atgaacgcgg taagttgcgt gaagatcaaa aaaggtttgc tgaattttta aacggtcaac
     9301 ctgtactata cggtgttgca cgatcagtgg aagaagcatt gaaaattgtg gaggaatcat
     9361 catgacaaca gaagaagtga ttcaaatgcg tattcgaagc cttcagcgtg agattgacga
     9421 tctggaacgg acaaaggcag tgatggtcaa tgaaacggcg aggaaggcaa tcgatttgca
     9481 catagagaat ttaagaaggg aaatccatcg attggaggaa tgagcgtgga taagaaagca
     9541 gcaatgaaac gaatcatcga actgacacat tctgagaatt ggcaagaaga caaagaaata
     9601 gttgcagaag tccaaaagct cggtaaatca atgtggagtg aaaaaaccaa acggagaaca
     9661 ccgagaaaaa ttgcaatctg gcatggtgac cgaattctag taacaggtac tgctgaacag
     9721 ttatctgaaa ttactggatt aagcaaaaac attatctggg atagagctaa acgcgagaac
     9781 gttgattcga aaggtcgtca atttaaacat tgggagaaga aataatggag gaactaatca
     9841 caaaagtaga gcagtgggca aaagataagg gattggatca agctgattcc agcaagcaaa
     9901 tgttaaaaac gatcgaagag attggggaag ttgccgcttc tctagctaga aaagatgaat
     9961 atggtttaag agacggaatt ggagacgtag tagtaacctt gattatttta gctatgcaaa
    10021 atgatatgga tttgtacgag tgtctgaacc aagcatacaa cgaaatcaaa ggacgcacag
    10081 gaaaaatggt agatggtgta ttcgtgaagt cgagtgattt ggaggacagc gatgaataaa
    10141 aaagtattaa ttgataaaca agcattgatt gatgaattaa tgaaaatacc tggtgtggga
    10201 tctaatagtg acgctttaga aacgattaaa cgtttcccat cgtatgaacc gcagaagcca
    10261 gttgtgccta agtttgtggc ggaactactc gactattatc gtcagtcaac agatgttgat
    10321 ttattagctt tattgataac tttccatgat tggtattatc gcaaaactaa agacggtgag
    10381 catgaagaag caattgattg gctagttgat catcctgaaa tttatatgcg tgcatggctg
    10441 tttggctacg agctcgagaa agagccgttg tattgcgtaa taatcaatgg tagatatctt
    10501 gtcaaagtat tcagcaatac aaacgttgtc atattagttc cagcagatga attttcacag
    10561 tacgttactc aaaaatttca attaactgaa aaacaaatca aagcaattga tgaaagatac
    10621 tggccgtttg ctgtgccagt ggatgaggtg gtagaaggat gagcgaccca tacattacta
    10681 aattagaaaa gttgtccgaa gattttgaga agaataaaaa taaactaact aaagataaac
    10741 ttatagaact ggttaggttg gttatcagag gagctattac aagagaaaag gcgcagcaaa
    10801 acatagaaat ttttgctcgt agtgtccgag atggattaga gaagaaagtt aaacaaaatg
    10861 aacaacagac accgcagaat aacaaaacta agaaaacggg aactgaatgt actaaagaca
    10921 aagtttgaaa aagaatatgg aatttcagca gaagaaacat ataaagtggc aagtcagtgt
    10981 gttgctgatg cgagcggcgc tattcgtaag tttggaattt cgatattaag tgatgatcgt
    11041 aaatgggagg aaaaagaatg aaactaaaag acggatttta ctccagcagt catggtatcg
    11101 gcggtttaat gctagatatg ccgacaaaga accctaaaac acgtgagaaa ccaaaattca
    11161 aagtcggtga catggttcgc tgtgaagcag aaggattcat ctatccattt tgtggatatg
    11221 tagaacatct ctataatcac tcagcaatca ttcgtattga aaacacgatg gaatgtgaca
    11281 agtggttagc gaaaagcaaa gagaatttag cagtagcgag attggt
//
LOCUS       DAHHZJ010000095        11187 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.096, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000095 DAHHZJ010000000
VERSION     DAHHZJ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.096"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(20..955)
                     /gene="murB"
                     /locus_tag="NBL02_001885"
     CDS             complement(20..955)
                     /gene="murB"
                     /locus_tag="NBL02_001885"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCD2572565.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1028..1948)
                     /locus_tag="NBL02_001886"
     CDS             complement(1028..1948)
                     /locus_tag="NBL02_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCD2572566.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2061..2813
                     /gene="xth"
                     /locus_tag="NBL02_001887"
     CDS             2061..2813
                     /gene="xth"
                     /locus_tag="NBL02_001887"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCD2572567.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3677..4210
                     /locus_tag="NBL02_001888"
     CDS             3677..4210
                     /locus_tag="NBL02_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HCD2572568.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(4700..5224)
                     /locus_tag="NBL02_001889"
     CDS             complement(4700..5224)
                     /locus_tag="NBL02_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD2572569.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(5217..5690)
                     /gene="tsaE"
                     /locus_tag="NBL02_001890"
     CDS             complement(5217..5690)
                     /gene="tsaE"
                     /locus_tag="NBL02_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCD2572570.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(5813..6793)
                     /gene="pta"
                     /locus_tag="NBL02_001891"
     CDS             complement(5813..6793)
                     /gene="pta"
                     /locus_tag="NBL02_001891"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCD2572571.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(6946..7626)
                     /locus_tag="NBL02_001892"
     CDS             complement(6946..7626)
                     /locus_tag="NBL02_001892"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCD2572572.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            7752..8621
                     /locus_tag="NBL02_001893"
     CDS             7752..8621
                     /locus_tag="NBL02_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCD2572573.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            8647..9162
                     /locus_tag="NBL02_001894"
     CDS             8647..9162
                     /locus_tag="NBL02_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572574.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(9209..9802)
                     /locus_tag="NBL02_001895"
     CDS             complement(9209..9802)
                     /locus_tag="NBL02_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD2572575.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(9827..10006)
                     /locus_tag="NBL02_001896"
     CDS             complement(9827..10006)
                     /locus_tag="NBL02_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572576.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(10083..10592)
                     /locus_tag="NBL02_001897"
     CDS             complement(10083..10592)
                     /locus_tag="NBL02_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572577.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(10688..11068)
                     /locus_tag="NBL02_001898"
     CDS             complement(10688..11068)
                     /locus_tag="NBL02_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCD2572578.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
BASE COUNT         3208 a         2287 c         1988 g         3704 t
ORIGIN      
        1 ttttaagttc cattacttat taattggcta aacttctgct tgtttctgat attgcttgtt
       61 tttctccaat gatccgcact tctgtttcta aagacacatc aaatttttct ttgattactt
      121 cttggatgtg cgcaatcaat tcaatataat ctgtcgctgt cgcatgatcg atattgacga
      181 tgaatccagc atgtttttca gagacttgtg caccgcccca tttcaggcct tgcaatcctg
      241 cttgttggat cagctgacct gtatagtgtc ctactgggcg cttaaagaca cttccgcatg
      301 aagggtattc taatggctgt tttgattgac gcaattcggt caactcatcc attcttgctt
      361 tgattgcttc ataatcccct gtttgcaaac tgaatcgagc ttccaatacg atagcacgta
      421 attcttggac tttactgtga cgataagaaa ataccatttc gtcttttgtc aaagttttca
      481 atgttccatc ttcaagcaaa agatccactt cagcaaaaac atctttgatc tctccgccat
      541 acgcacctgc attcatataa accgctccac cgacacttcc cgggatgccg caagcaaatt
      601 caaatccagt caatgattct gccaaagctg cataagtcgt atcaatcagt gaggcacctg
      661 cttccgcaat gaccatcgtt cctttgacat gaatctgttt catttcagtc agcatgatga
      721 ctacaccgcg aatcccgcca tcttgaacga tcagattgct tgcatttcct aataccatcc
      781 aaggtatatc atggattcgg caataatcga tgatttgctt tacttcttct tgttttttcg
      841 ggaatgcaag gacatcagct ggtccacctg ttttcgtaaa cgtatagttc atcaatggtt
      901 catcaaacaa tatagtaatc gtggctaagc tttcaactat ttcttcttta ttcactgagg
      961 attttccttt cttttctcaa acgtcctcat tattctatca cgcttaatac aatcttgcca
     1021 gtcttgttca aagttgatga cggtagtgat ctttaagcac ttcctcacat aattcatgac
     1081 ttcttacaac ttctttttgt ttaagcaatt ctgtttccga tttacctaaa cggatctgag
     1141 aaaagaaggc ctctaccatt ggctcaaacc cgcgtttgac tagcgtgttt gtccagtcgc
     1201 caaattcttt cacttttttt ccatcagcat cttgaatgac aagctgggtc agatcgttta
     1261 cttcataagt accattttta cttgttactt gatatttttc tatattagcg ccacttgcta
     1321 aatccatcgt taaaatagcc gtttgatttt gtgtttctaa ttgtaaaatg gcatattcca
     1381 aaaattcctt tgtttctctt atctggtatt ttgttctcaa aatgggttca tctaataaat
     1441 acacagctgt atcaataaga tgtaaaaaca aatcataaag gacgaactct gtcgtttctt
     1501 ttgccgctat tcgatttttt tgcaggtgga tcaaccgctt atctgggata cgtttcaatt
     1561 cttctaccat aggagcaaag cgccggttga aaccgatcat caaaattatt tggttcttat
     1621 cagctaattc ttgcaattca cgaacttcat ttagatggac actcagtggt ttgtcaatat
     1681 aaacatggat accattttct agacattttt tggctaattc ataatgtact tttgttgctg
     1741 cgtggatcat acatgcttcg attttttctt ctaaaagctc atcgatcgtt tcatatagat
     1801 gttggaaccc gtactctttt tgaattcttt cttttgtttc tttatttctt gttgcaaaat
     1861 aaaaatcaag tgttccttgt tttttcgcat aagttggtaa atatgctttt tctgcgatat
     1921 tccctacacc gattacacca atcttcatct actttcctcc tggatcattt agttactttc
     1981 atcttacacc attttttact aaaatgaggt acaatagtat cgcaaaggta aggaaattaa
     2041 ttcgaaggag tgtgctttta ttgaaattta tctcttggaa cgtaaacggg cttcgtgcca
     2101 tcgtaaataa aaatttttta gaagtttttc atgagttaga cgccgatttt ttctgtctgc
     2161 aggaaacgaa actccaagca ggacaaattg atttagatct tccaggttat tatcaatatt
     2221 ggaattacgc ggaacgaaaa ggctattccg gcacagcgat ttttgctaaa aaaccagcat
     2281 tgaatgccac ttacggaatg ggtatcgata tccatgatac agaaggccgt ctgattactt
     2341 tggaatattc cgatttcttt cttgtaacct gttacacgcc aaattctcaa agcgaattaa
     2401 aacgtttaga ttaccgatta gaatgggaag aagcctttta taattatttg gaaaatctaa
     2461 aaaaacaaaa accagtcatc gtgtgtgggg atctaaatgt cgctcatcaa aagattgatt
     2521 tgaaaaactg gaaaacgaat caaaaaaatg ctggattcac tccagaagaa agagcggctc
     2581 tttctcgact gttggataac gggtttatcg atacattccg ttatttttat ccaacccagg
     2641 aaggtgtcta ctcttggtgg aactaccgat tcaattcaag gaaaaacaat gctggttggc
     2701 ggatcgatta ctttttgacg agcaaagatt tagagccagg actagctgat gcaaagatcc
     2761 atacggacat tatgggcagt gatcattgtc cagtagaatt agatttgaaa taaagaggtt
     2821 gtgaaaaagt cgtttagttt cgagtattaa gaaaaatttt gtgaaaatgg ctttccacat
     2881 tttgcataaa tttggcataa taccgagaaa ctggcttttg aacactgttt atcgagagat
     2941 tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca aacaaaaata gttcctcata
     3001 tttttcgttt ggttgggctt attaccgcag atacaagctt ttgaacaccg tttattaaga
     3061 ggttgtgaaa aagctgcact gcatcaagta ataagaacag ccaaacaaaa atagttcctc
     3121 atatttttcg tttggttggg cttattaccg cagatgcaag cttttgaaca ccgtttatta
     3181 agaggttgtg aaaaagctgc actgcatcaa gtaataagaa cagccaaaca aaaatagttc
     3241 ctcatatttt tcgtttggtt ggacttatta ccgcagatgc aagcttttga acaccgttta
     3301 ttgagaagtt gtgaaatcag cgtcctgacc tgagtattag agaaacaaat ggcaaaatag
     3361 ttcctcatat tttgctattt tttgagctaa taccgagggt cagctgattg aacaccgttt
     3421 agtggagaag ttgtgaaaaa gtcgcctact ttcgagtcgt aagaaatttt tttgaaaatg
     3481 gttcctcaca actttgcata aatttgactt aataccgaga agtcatgatc cccatctaac
     3541 tccaaatcac aaggaatgag ttcaaaaact agcttttctc aacaaatatt acataaaaaa
     3601 ttctatgttt tttgttaaat ttatttgctt ttttcaaaag tatgttacag taatttccca
     3661 aggaggtttt acagaaatga attttgttgc catggatttt gaaactgcca atcatcagcc
     3721 ttatagtgct tgttctcttg cgctagtcat ggtaaaaaat agccagatcg ttgatgaatt
     3781 ctatacattg atacagcctg agactccttt tttctggcga aatgtccaaa ttcacggaat
     3841 ccatcaagaa gacgtacgca atgcgccgaa attcccagaa gtctggcaaa ctatccaacc
     3901 atattttcaa aaaaatcgtt tagtcgttgc acataatgct gcttttgata caaaggtctt
     3961 agcgggatgc ttagattatt atcatcttga acagcctaat tatctttctt tatgtacagt
     4021 gaaaagtagc cgtcgcctgt ttcctgaatt tccaaatcat cgattgaaca ctgtgtgcga
     4081 aaatttgaac atccaattag atcatcacca tgatgcattg gaagatagtc gtgcatgtgc
     4141 ggaaatcctg ctttatcaag aaaaaaaatt cgggacagat ccattgaaaa aacttgtgac
     4201 tataaagtaa aagaatactt atgaaacaca caaagaggtc gtgagcctgc cgtttcactc
     4261 cgagtcacaa ggagcaattt caaaaaatag cttctcatgt ttattaaata ttgtgactgg
     4321 tgaccgagga gctggcagga gaacaccgtt tattggagag gttgtgaaaa agctgttctg
     4381 catcaagtaa taagaacagc caaacaaaaa tagcttctca tatttttcat ttggttggac
     4441 ttattaccgc agatgcaagc ttttggacac cgtttaccaa gaggttgtga aatcagcgtt
     4501 ttgacctgag tattagagaa acaaacggca aaatagttcc tcatattttg ccgttttttg
     4561 agctaatacc gaaggtcagc tgattgaaca ccgtttattc aaaaaaaatg gactgcgaca
     4621 cgacttttgt cacagtccct tcgtgtgttt ttctgccttc tcaaaaggat gacagtttct
     4681 cactcttttc ctatttcttc tattcaatca aataactcat tttatataca tctaaaaatt
     4741 cacctagatc tgttcttgcc cctctaggca tgaccgcttc gatttggaag cccatttttc
     4801 ggtaaaggtg tacggctctt tcattacgaa cttgaacgtc aagctccaat cgaaaaagta
     4861 cacccatttc cttcgcccaa ctgatgattt cttctagcat aagagtgcct agtcccattc
     4921 cccaatattc ctgcagaata ctgataccga cttctcccac gtgagacaaa cgaaattgac
     4981 tgtctgcctt gacagatgct gttccaataa tcgtcccttc atagattgca agcagcaata
     5041 aattattatt gctttcgtat atataatcca actcttcttc taaggtttct ggtggtaaca
     5101 ggagttctgc ttcatccaaa actaaaaatt ctgtctcctt attaacttgt cccatcatca
     5161 aaagaatgtc ttttgcatct cctggcactg cttcccgcaa gatgatccca acttcttcac
     5221 tcatgatttt cagcccattt ttctagaatt cgctgtttga aggtttcact ttgctccccg
     5281 tatgagatta gtttcacata ccgcttttct aaatctgtat cacttttttc taaaatcagt
     5341 tccaaataat cctccggcag tgcttcttct aatagatttc cccattcaac aacggaaagc
     5401 ccatcacctt caaaatattc atctaaccct aaatctgctc cgctagctgc tacccgataa
     5461 atatccatat gatacaacgg aatccgacct tgactatatt ctctaataat cgtgtatgtc
     5521 ggacttttga tcatttgcgt tatccctaat ccttgggcaa ttcctttcgt caacgttgtt
     5581 ttacccgcac ctagatcacc agtcaatatc agattgtcac ctggcattgc ggaatgacca
     5641 attgcctctg cgaattgttc tgtcattgtt aatcctgata gttcgatcat ttttcatctt
     5701 ctcccgctct tctttctttt tgccagtata tcgcgtaatg aagttttttt aaagaaaaaa
     5761 tgaactagtc aagaacagca gctgctgttt ttggctagtt cgcgtgtatt tgttaatcaa
     5821 tcaatgtttg tgctgctgtg ataatcgata atttatatac atcttcttca tttgctccac
     5881 gagacaaatc agaaaccggt ttgttcaagc cttgtagaat tggtccgatt gcttcaaagt
     5941 taccgaaacg ctgagcaatt ttatagccga tatttcctga ctgcaattct ggaaatacaa
     6001 agactgttgc ttgaccagct acttctgaat ctggcgcttt cagttgtgca actgaaggaa
     6061 cataagaagc atcaaattgc aattcgccgt cgatcatgta ttgtggcgct agttttttag
     6121 cgattttagt tgcttcaact actttgtcta cttctggcga tttcgctgag ccttttgtcg
     6181 agaagctcat cattgctact tttggttcga tatcgaacaa ttctgctgtt tttgcagaat
     6241 ctacggcgat ttcagccaat tcttgcgcag ttggattcac gttgatcgca caatccgaaa
     6301 agacatattt ttcttgatct cttccacgta ccatgatcat cgctccgctt gtccgactga
     6361 tgccaggctt tgttttgatg atctgaagtg ctgggcgaac agtgtcgcca gtcgaatgga
     6421 tcgctccaga gaccattccg tctgcaatcc ccatatacgt aagcatcgta ccaaagtaat
     6481 tcacatcttt taaaatcttt tctgcttgtt ctttggatgc ctttccattt ctacgttcaa
     6541 caaaagtatc caccatttca tcccacttat cgtaattatc agggctgatg atcgtaaagc
     6601 tagatgtttt gattcctctg gcatgcgctg catttcttat ttcttcttcg ttccctacta
     6661 aaatgggttc cacaagttct tctgctttca accgggcagc tgcgcctaaa atacgagcat
     6721 cagtagcttc cgggaaaacg attttgatcc cacggcgaac aattttaaat cttaaactat
     6781 caaacaattc cacaagatga cctccagacg tttttatatg tattatcata cactattcca
     6841 gaaaaaaaga acagtttatt ttatctgtat cagtcaaaaa tgctggaaaa attaagtttt
     6901 tcacatacaa agcgaactgc actataacag gaaagcgctg ttttattaca ctgtttcagg
     6961 cagttgccaa tcaatcggtt cttctcctaa cgcaaggagc gcatcattcg ttttcgagaa
     7021 gggtttggaa ccaaaaaagc cacgacttgc agataacgga ctgggatgtg gagatgttat
     7081 gatgatatgg cgactcttat cgatcatttt gattttttct tgtgccggtt ttccccacaa
     7141 gataaacacg attggttttt cccgttcatt taatttttca atgatcgtgt ctgttaatct
     7201 ttcccaacct ttcccgcgat gagaatacgc ttgcccttcc cgaacggtaa gaacggtgtt
     7261 gagtaaaagg actccctgtt tagcccatga cactagattt ccatgttgaa caggtgcgat
     7321 tccaagatca tcataaagct ctttatagat atttcttaac gatggcggga tctttacgcc
     7381 aggttgcaca gaaaaagata agccatgagc ttgattttct ccatgataag gatcttgtcc
     7441 taaaatcacc actttaactt cttcataagg agtcaattcc aatgcttcat agatatgata
     7501 catatctgga tggatctttt gggacgcata ttcttttttt aaaaattctc ttagatgcag
     7561 ataataatct tttgaaaatt ctgaggacaa aacttcttgc caactgttat gaatgatttc
     7621 tttcaaaaac tccacctcct aataaaaaga ataactcaca ttttcttcaa tcacaaggga
     7681 caatcttcac cctcagcact tgattaatat ggtaaactag gaacaagaag cagaaaggaa
     7741 cgataatcat tatgatcaaa ttaattgcat cagatatgga cggcacatta ttagatgccc
     7801 atatgagtgt ttctcctgaa aatgccgagg cgatacgttt tgcaaatgat gccggtgttg
     7861 agttcatggt cgctactggt cgaaactatc aagaagctcg cgcagcttta gatgaggtag
     7921 gaatcgattg tgcgatgatc actttgaacg gggcacaggt atttgataaa gaaggaaatt
     7981 cattgtttac cgtgtcgatt ccaaataatc aagcaatctc tgttttagat attctagatg
     8041 ctaacggtat ttactatgaa gtagcaacca acgatggtct ttattctgaa aatcaagcga
     8101 aacgaattga aagttttgct tccatggtgg ctacccattt acctcacctt acgtataaaa
     8161 tggcgattgc catggcctca gctaagctgg agttattgca catcacatac gtggacagta
     8221 ttcgtgctat tttggaagaa aagaatctgg aagttttaaa aattatttgt tttcatacag
     8281 aagggccagc tgtattaggt ccagttggaa agcagatttc tcttttaggc gatctagccg
     8341 ttacttcctc tggtcaaaac aatatcgaag tgaaccataa aaacgctcaa aaaggaattg
     8401 ctgttgccca tgtcgctcac gagcgtggga tttctttaga agaagtcatg acgattggcg
     8461 ataacttcaa tgatgtcagc atgctgcaag tagcaggagt cagtttcgca atgggaaatg
     8521 cagaaatcga agtaaaagat tatgccaaat atacaacgga cacaaacctt gaatctggcg
     8581 taggaaaagc catattgcgc gcgattgcag aagacttgta gttttatcta gaaaagaggg
     8641 atatagatgt cagaattttt cattcaggaa aagcagcttg gacgtgcaac tcggacaatt
     8701 gtcaaaaacg aaaaaggaca ttccctgttt ttgatggttg gtcgctgggg aacaaagggc
     8761 gatgcattgt ctctttatgc catgaatggc gatctcgttg ctcacatcaa gcagataagc
     8821 ctgacattcg gtacgcgttt tgagctgtat aaagagttcg aaaaggtggg aaccatgcag
     8881 aaaatattca attggcccgg tgacttttat tatattcgta gacttcactg gacagtccaa
     8941 ggtgatatct ataatcatca atatcaaatc catcatttca atcagcaaat catgaaaatg
     9001 gataaagcaa cacttttttc aggagattac tatgtattgg atgtctcaaa tgatgaagat
     9061 gctccagttt gtatttgtat tgctgcggtt ttagattact ggctctaccg taaagacaaa
     9121 agtcgcgata agcctgtttc ttaccgactt aacttaagtt aaaaagatgt acctgaaatg
     9181 cggaaatacg catttcaggt acatcttttt agatattctt ttgcatatga tcataaagat
     9241 gaccactgat catccgctga ccgacaactt tgaagtcatg gcgttcgtat aattttttag
     9301 cctttggatt tttttcatcc acacttagac cgatgacgga gcgattagct ttcttcgcca
     9361 atttaggtaa tgcttctaat aatcgcgaac cgatgccttg tcctctaaaa tcttctgata
     9421 cactgataga gtccaaatac cattcgttgg gaaacgtttc tttatcaatg aacaaacgtg
     9481 tagacgcatc tagctgatat ttctctaaga taggtaccag tggctgatcg atgattgatt
     9541 cttcttcatc tgtataaccg taagcaactc ctgcaacttt accgtctatt tcttctacga
     9601 tacctcgtga ataactgtaa cgataatttg gatcagtgat tgcttcttct aaaatctcta
     9661 aagtcttttg ttcccctgtc tgcttcaaaa atggcagttc catatctttt agaatcacta
     9721 agatcaatgg ggcgatttct ggagcatctt cttttctcgc aaaacgaatc atagtttcct
     9781 ctccttttct ccattctaac atgcagaaaa agaaatcgca aaatcgttat tttaaaaaat
     9841 cttcaatctt ttcagtaatg aaataaccat gagtaacgat tcttgccgct tcagttcggc
     9901 aataagggca gacgactggc atttctttat cattatgttc gactacatta ttgacttctt
     9961 cgtatgcaaa tttttttccg caattagggc atttcatttt ttccatgtta tctcaacttc
    10021 ctttcatctt tactctctta gcttactctt atattgtaaa aaagagaagc aatatccttc
    10081 ttctatgcag atatccatac ctgacggtcc tgttctccat ctattaatcg gactttataa
    10141 taaggacgtt catctttgac tagaagtgtc cactctttca cttttgcttt tttcccgtag
    10201 tagctgactg cttccttcat cgctgcttgc ggttgtttga actcttccgt tttttctata
    10261 tcaaaaagat gttctgcggc cttttccgtg tcatttcgta cggttgtttt cccagtggat
    10321 ggattgactg ctatgacttg tccttcacaa aaaaactggt attcatatgt tgcattcgta
    10381 ggattctcca aatctttttc aactgcaaaa tgaatagcag atatgttatg ttcttttgtt
    10441 tcgtttttta cgattgctgc tgcttttttc aatgagatcc gatattcctg gtcactaata
    10501 tcatccatgt gaaaagcggc attgagcagt cccgtcatac cggtaatccc tattacgatg
    10561 agggcagcaa caccaaacat catttttttc attcttcatt ctcctgtttc ttttttttaa
    10621 agaatatcaa gaaatgaacg aatgaaaaat cgaatgaaac gaaaaaaaag tgacaatttt
    10681 gttatcttta ttcgtgcagt tctaatggca atccgtctgg atcaaagaaa aacgtcattt
    10741 tttttcctgt aaaggtatct tctctaatcg gttctgattc gatccctaag gactgcaatt
    10801 ctgcaattgt ttcttcaata tcgtctactt tgaaagcaag atgacgcaag ccacaagctt
    10861 ctggatagtt tggtcgttgt ggcgcattag caattccaaa aatctccaat tcagcacttc
    10921 ctagtttcag atctaattta tagtcatctc gttctttgcg gtaattttct ctgattactt
    10981 caaatcctaa tttattaaca taaaaatcac gtgatttctg ataatctgaa acaatgatgg
    11041 ctatatgatg gatcgttgta aggttcatat gagcactcct attcttcgtt taagctcgga
    11101 tctatattaa tctccagttc cattgtaaat gtgtcagatt gaaaaaaata gctgtttttc
    11161 ctttttataa gaaaaaaggc aaaaaaa
//
LOCUS       DAHHZJ010000096        11159 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.097, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000096 DAHHZJ010000000
VERSION     DAHHZJ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.097"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            145..1431
                     /gene="efmA"
                     /locus_tag="NBL02_001899"
     CDS             145..1431
                     /gene="efmA"
                     /locus_tag="NBL02_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HCD2572579.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(1569..2168)
                     /locus_tag="NBL02_001900"
     CDS             complement(1569..2168)
                     /locus_tag="NBL02_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HCD2572580.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            2572..3693
                     /gene="mnmA"
                     /locus_tag="NBL02_001901"
     CDS             2572..3693
                     /gene="mnmA"
                     /locus_tag="NBL02_001901"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCD2572581.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            3939..5360
                     /gene="arcD"
                     /locus_tag="NBL02_001902"
     CDS             3939..5360
                     /gene="arcD"
                     /locus_tag="NBL02_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HCD2572582.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            5374..6492
                     /locus_tag="NBL02_001903"
     CDS             5374..6492
                     /locus_tag="NBL02_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HCD2572583.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(6552..7844)
                     /locus_tag="NBL02_001904"
     CDS             complement(6552..7844)
                     /locus_tag="NBL02_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HCD2572584.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            7976..8554
                     /locus_tag="NBL02_001905"
     CDS             7976..8554
                     /locus_tag="NBL02_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCD2572585.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            8683..9060
                     /locus_tag="NBL02_001906"
     CDS             8683..9060
                     /locus_tag="NBL02_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HCD2572586.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            8993..11065
                     /locus_tag="NBL02_001907"
     CDS             8993..11065
                     /locus_tag="NBL02_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCD2572587.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
BASE COUNT         3598 a         1995 c         2416 g         3150 t
ORIGIN      
        1 tttgctgatt tattgtttct aagtaaagaa atttaaattg aaacgattgt tatttaggga
       61 aaaaggtatt ttttgaaaaa agaatcccgt ataatgattg ttgtgcaaac gctatacaaa
      121 atgaaaaaga aaagagggat gtttatggaa aatgaacagt cggtcgtttt aacgaattgg
      181 aagagaaatt atttgttctt tttatcggga cagtttttgt caggaatcac tagtatggtc
      241 gtccaatatg cgatcatctg gtatttgaca agagaaaccg gttcggcgac aattttgagt
      301 tttgctactt tactaggaat gattcccatg gttttactaa gcccatttgt aggaccgcta
      361 gtggatcgct gggataaaaa agctcttttg attgtcacgg atattattgt agcgattttc
      421 gcgcttattt tagcagtagt cggaacgatt tcagaatcct tcccgatttg gctagttttt
      481 gtgtctttgt ttatgcgttc ggtcgcacaa acattccaaa tgccaacgat ccagtcgatt
      541 atgccgacaa tcgttccatc ttctcacata acaaggacga atggacagtt aggaatggtc
      601 caatcagcga atttcatcat tgcgccagct ctaggtgcgg ctttgttttc agtcgttcct
      661 gtcaattacc ttattttatt agatgtattg ggggcagttt ttggtgtcgg cttattgatt
      721 tttgtgaaga tcccaaaagt ttctcctgaa atattggaag ttccgcttac tatttttaaa
      781 gatgcaaaat ttgggctgca gcaattgatg gataataaag ggttatggta cataacgatc
      841 aatggtgcat ttgtgatgct gttgttcatg ccagcaatca gtctgtatcc attgatgacg
      901 ctagattact ttggtggttc cgttggacag gcgggagcag tggaagtagt ctacgcagtc
      961 ggaatgctct taggcggtgc actgatcagt tttataggaa catggaaaga ccgaatgaaa
     1021 cctattatta tagcctacat cattatgggc ctgacgatcg gtgcaagcgg attggttcca
     1081 aacgatagtc aagggttctt gtactttctt atcttgaatg caggagcagg ttgtgcaacg
     1141 ccatatttca atacactgct gatggcaatg atccagcaga gttatgaatc gaatgtttta
     1201 ggtcgtgtct tagggaactt caactctttg atgaacctgg caggtccaat tggattatta
     1261 tttgcaggac cattagctga tcgtttaggg gtagaaaaaa tgttcttgtt ttccggaatc
     1321 gggattttat tatgcggaat cgttttattt ttaacgtcag ctgcccgaaa ttatgataaa
     1381 gaattacaga aaaaactggt aaaagaacac catgagcaaa aagacgaata gtatggaatg
     1441 gagttgtgac agaagtcttg ttacaactcc atttctgatc ctaatgttcc tgtggcaata
     1501 agcctactca aatgaaaaat atgagtaatt atctttattt gtttaatact tgatataggg
     1561 cagttttttc acgacctatc gtacttcctc tttgcttcat agatttcttt gatttgttgc
     1621 tcagataacg gagtgccaaa tggtagtttt tcctttaaga gttctctttc cggtgcgtct
     1681 acgcctacat tgacatcgtt ctcgataggt acgctgtaat gatggatatg tccatgaaga
     1741 ttgcggatag tgtcgttagg cccgagcaac atcggataat gagtcaagta atattgttga
     1801 tggtcgaatt taacgatggc acctacatca taaaaataaa atttgggact tccgtcagta
     1861 agcctaggat catttttttc taaataacgg aaaaaagcgc gactgtcatg gttccctttg
     1921 atcaagtgga ttgttccttg cagttgagtg accagttcca aaatttctgc atttgctttt
     1981 tcatattgcg gatgcatagc aaggtcgcct agatgataaa catggtcggt ttcttttatt
     2041 actgcattcc agctagtgat caactgttgg ttcatttcat ccacatttgc aaagagccgc
     2101 ggagcaaagt cgttttttcc taataagcgt tcatggaaaa agtgcatatc actgataaag
     2161 taattcatgt atcttttgcc tccctttttt cttagagtaa acaaaatcac ttatctgagc
     2221 aactcataac atctaaaaag aaaaggttgt aaatgagacg tgcagcttta agcgataaga
     2281 agcaattttg aaaaatagtt tttcacaatt tataaaatta ttccttattg ccgaaaggct
     2341 ggcccttttt atgagtatgc caatttttta tatcgaatgc tcaaaagatg agtgattttt
     2401 tggttgacta tcatgtttgt gtactgaata ggtccagtag aacagtattt ttacgacttg
     2461 ataattaagg gatgaaattt tcagaaatat gacgtataat gtaaaatact gaaaagttgc
     2521 gaccttcaac tcgtagatgt ttcagaatct aattgaaatg atggtgatat catgacagac
     2581 aacagcaaaa cacgcgttgt cgtagggatg agcggcggtg tcgactcatc tgtaacagca
     2641 cttttgttaa aggaacaagg atacgacgtg atcggaatat tcatgaaaaa ttgggatgac
     2701 acggacgaaa acggtgtatg cacggccacg gaagattata aggatgtagc gaaagtagct
     2761 gctcaaatcg ggattcctta ttactctgtg aattttgaaa aagaatattg ggatcgtgta
     2821 tttgaatatt tcctagccga ataccgcgca ggacgtacac ctaacccaga cgtgatgtgc
     2881 aacaaagaaa tcaaattcaa agcattcttg gattatgcaa tggatcttgg cgccgattat
     2941 gtagctactg gacattatgc gcaagtcact cgcgatgaaa acggcgtggt ccacatgctt
     3001 cgtggagtag acaataataa agatcaaact tacttcctta gccaattatc acaagaacag
     3061 ctttcaaaaa cgatgttccc tctaggcggc atggaaaaat cagaagtacg ggcaattgct
     3121 gaacgcgcag gccttgcgac tgctaagaaa aaagattcaa cgggaatctg ctttattggt
     3181 gaaaagaact tcaaacaatt tttgagcaac tatctgccag caaaaaaagg aaatatggtc
     3241 actcttgatg gtgaagtcaa aggacaacat gctggcttga tgtattatac gattggtcaa
     3301 cgtcaaggac tagggatcgg tggcggagga gattcgcaag aaccctggtt tgttgtagga
     3361 aaagatctag cgacaaacac attatatgtg gggcaagggt tccatcatcc tgcattgtat
     3421 gccacaagct tggatgcaag tgaaatccac tttacgacga atgaaccaat gccaaaagaa
     3481 ttcaagtgta cagcgaaatt ccgttatcgt cagcaagacg ttcctgtgac agttcgctta
     3541 ttggatgata atcgtgcaga agtcgtattc gacgaacctg tacgtgcaat cactcctgga
     3601 caagcagttg tattttatga tggaatggaa tgtctgggtg gtggtttgat cgaccacgca
     3661 tatcaagaaa caaaagtttt acaatacgtt taagcgatat taaatgaatc aaaacagcta
     3721 tggctaacaa aagaaacacc ttttcttttg gatagcatag ctgttttttt gcaataaaat
     3781 taatttttat tcattttata cttgaaatac ttgtgtaaaa agttcataat atccaataat
     3841 atgaataaag ttgctttttg ttaagcaaga gcagttgcaa aatagaaggg aatcttttgc
     3901 aagtgatttg ttcaatttta taagtgaggg tgatggaaat ggaaaagaaa tcgacgggta
     3961 ttagcaaatg gggactggtt gctctggttg tcagttcgtc aatcggaagc ggtgtgtttg
     4021 ggatcacaag tgatctggct agttctgcag ctccagggcc agcgatcctt tcatggataa
     4081 tcgttgggat tggtatttta gctttggtat tatcgctgaa tcatttagga gaaaagcgtc
     4141 cagatctaga tggtggtatt ttcggttacg cggaagcttc atttggtaaa ttaggtggct
     4201 tcatcagtgg ttggggttac tggttgtctg cgtggctggg aaatgttgct tttgcaacga
     4261 tgctgatgag cgccatggga gaatttttcc cgatattcaa aggtggacaa aatgttcctt
     4321 ctattttatt tgcaagtatt tttatttggg gtctgacttt attagtaaac aatggagttg
     4381 aaggagcgag cgtcatcaat acaatcgtga cgatctgtaa attagtgcca ttattcctat
     4441 ttttaatatt tgtatttatc gcatttaaag tgaatatatt cactgctgat ttttggggta
     4501 acgtatctga taatctagtg aatggaacag ggcagcaagg aacgatctgg ttgcagatta
     4561 aaggttgttt gatggttatg atgtgggtct ttgtagggat cgaaggcgca agtgttttag
     4621 ccaaccgcgc gaagaaacgt tcagaagccc agcaagcatc gatcattggt ctgctttgtt
     4681 tattgtttat ctatatcctt gcttcgatct tgccatacgg cgtcttatct caagaagagt
     4741 tagcaactat ttcacaacct gctatggcaa atattttaaa aggaatcgtg ggaaattggg
     4801 gagcagcgct gatcaatatc ggcttgatta tttcgatcat tggttcttgg ttgtcttgga
     4861 cgatgctccc agcagaaaca acgatgttga tggctcgaga cggtctgttg ccgaaaggtt
     4921 ggggaaaaac gaataaaaaa aatgccccaa cttattcgtt agttgttact gcaatcttga
     4981 ctaatctatt cttattgact ttcttagtta ctgattatgc ctatcaattt gcctattctt
     5041 tatgtacagc ggcaattttg atttgttatc tacttgtcgg gatctatcaa gtccagtttt
     5101 ctttaaaaga aggaatcaaa aaacaagtag tgatcggtgg gattgctgta gtttttgaat
     5161 tgatgggcat cattttagca ggattttctt atgtcctttt atgtagtatt gcctatcttc
     5221 ctggtttttg cttctactgg atcgcttgtc gtgaaaccaa acatcaaatt acgggaaaag
     5281 aaaaagtaat gattgggctg atttgtgtgg ccgcactgct ttcaatcggg ttgcttgctg
     5341 taggctggat caagatataa gggggaaaga aacatgaaaa tcgcaaattt cggtgtagaa
     5401 gaatggctaa ataaatggga aaagaaagcg gtatatgata ttgcacaaag ctcgatcgaa
     5461 gcattgacat tagacgaact cgttggctta gatgggacta gcgtaagtaa atttttcgaa
     5521 aaacgcaaaa acgaaccatt ggactatggc tggatcgaag gatcagatgt tttcaaacaa
     5581 gaagtcgctt ctctttatca aaccgtcgat ccggaaaaca tcttacaaac gaatggagcg
     5641 actggtgcta atcttttagc tctatacgcg ttagtggaag caggagacca tgttgtttcg
     5701 atgcttccta cttatcagca attatatgac atccccaaat ctttaggtgc cgctgttgac
     5761 tttgttcatt tgaaggaaga agaagattgg cagtttgatt tagaacagct agaagcctta
     5821 gtcaaacctg aaacaaaaat catctgtttg aatagtgcaa acaatcctac aggcacattg
     5881 ctagatcgga aaactttaga aaaaatcgca gaaattgctc gtacagtaga tgcatatgta
     5941 ttgatagatg aagtatatgc gcctttaacg gataaaggtg aattcttgtc gatcgtagat
     6001 gtctatgaaa aaggtatagc aaccaattcc ctatccaaaa cctactccat tccaggtatt
     6061 cgtattggct ggacagctac tggacctgag ttggcagaag tttttcgaaa gtatcgagac
     6121 tacacgatga tttgcggcgg agtcctgtca gatgatcttg ctgtacatgc attgaagaat
     6181 aaagaaaaaa tattggcgcg aaacaaaaag atcattacag agaaccttgt gattttaaaa
     6241 caatgggtag ccaatgaacc gaaagttgaa cttgtcgctc ccaattacgt ctctacttct
     6301 ttcattaaat tagccatcga agaagatgac cagacatttt gtatcaattt attagaagaa
     6361 acaggagtac tattagtacc aggaagtgcc tttgatttac cgaagcatgc ccgactaggt
     6421 tactgttgta aaaaagaaac cttggaaaag ggattattcc tcttatctga gttcttgaaa
     6481 aaacaggcct aaacaaaaac actgcaagga cagtctgtga caactgttca agcagtgttt
     6541 ttcatttacg cttattgtct atgagaagga gagtgccctg aagctatgga gactcctgaa
     6601 gctttttctt ctgcagtttc ttgctgatgg atctcttgtg tattgtgtcg tacaagcaag
     6661 aagttgacca aaacaagaag agaagtcgaa agaaagacgc cgcgataacc aaatgcactg
     6721 gaaacactgg agccgatcat aggccctact acgttaccag ttgcttggaa agattggtta
     6781 taactgaaga tccgtccagc agcttgatga ggcgaatatt ttgtgatcaa tgcttggaca
     6841 gcagggagca gacaagcatc agagatacca atcaaaaaac gtaaggcagc taattgccaa
     6901 acattcgtga cgaatgccat tgggatataa accagaatcg caaaacccaa accaatcgca
     6961 agtattcttt cactcccaat cttgtcacct aagcgaccaa atcttggagc ggctatcaaa
     7021 gtggcaatcc caggtatcga agcaatgatt ccgctgatca acgtaacatt cccatgtcca
     7081 tgcagcaatt ggcgaatgta cagactgatg attggactaa tggaattgtt ggatgcttgg
     7141 atgatcatcg ttgtgacgaa cataccgatg acgacgtgtg gatatttcaa gtctttgaaa
     7201 atctgtttgg cagaaaccat gttttctttt ttcactggga caaatttttc atggacgaaa
     7261 aacagactca acaagaagac gaaaaataag atgatccctg taatgaaaaa ggtagggcga
     7321 tagccaaacg ctgaagcaga gatcccgcca agaagcggtc caagtaatgt tcctgtaaca
     7381 gaaccagttg ctaaggttcc aagaacttga ccactttttt cttttggcgt cccagttgcg
     7441 acaagggcag tggcattact gatataacca gaaaaaatcc cttgaagcag acgcaatgcg
     7501 actaattgat acacactagt cacgcaaccc atcaaagaaa tgacgattgc cattcctaat
     7561 gaagcacgca acagcatcaa ttttcttcct ttttggtcgg ctaattttcc ccaccaagga
     7621 gagataagcg tagtcaccaa gaaagtagat gaaaaagtca atccgctcca aaagttcaat
     7681 tgtgacgtac tataattacc taacgtatct atgtaaagtg acataaaagg catgactaaa
     7741 ctaaatccga taccagccat aaatgttcca aaccataaga caaacaagtt ttgttcccaa
     7801 gtttctcttt tcctaaataa tttttccttt attcttgcag ccaattagac tccttctttc
     7861 tgaaaatttt catctacagc tctttattat aaactacaat atgtagttta tcaatgaaag
     7921 tctctttttt ctctttcttt ttcgtggtaa actgaaagag tgaaggattt attttatgag
     7981 aggaaacatg gaaatgaaaa aaagaaatct gtctcaagag aaaatcatcg actgctttcg
     8041 agaactagca gaagagatgg atgttcagca aataaccttt cagcatttag ccaaacgctt
     8101 ggatatcaag gctccctcgc tgtataacca tttcaaaaat attcgtgatg tcaaaacagc
     8161 gctaacagcg aaacttctgc aggaacttaa tgaccagctg cgacgggctc ttgtgggaaa
     8221 aagtggtcag gaagcactgc agatctatgc agaaacttat cgatcctttg ctttttcaaa
     8281 tcaagcagtg tatgagttac tgatcagtgt accgcatacc aatgaagctg ttttattaga
     8341 tggaatccat gaaacgaatc agatcatttt gcagatattt gaggcttttc aattaacgaa
     8401 agaagaaaaa gttcatcgaa gcagggaact tcgtagtatg atccacggtt atctttcctt
     8461 acgtttttta ggatacttta caaaagaacc aacaatcgat ccagaagaca gctatttttg
     8521 gatgatcaat aactttatcg ctactttgcc ttaaacaatc agattcattt gtaacaattt
     8581 taagatgttc attaaatgac ccttttataa gagaaaattc ttgcaacttt cttgaaaagt
     8641 gactaagatg agtctagaaa agctgaaaga aggagcacga gtatgtatca agtaattacc
     8701 atgttcgggg acaatgagcc ttggtggttt tttgaagact gggaagaaga tattgaagaa
     8761 gaagaaacat tcgagtcttt cgaagaagca cgacaagcgt acgaaaaaca atggaacgaa
     8821 atccatcaaa attatgaata tatcaatgca aaaagtaact ttctcagcgc cttttggaat
     8881 gacggagatg agcgctggtg tgaagagtgt gacgatgatt tacagcaata caagggactt
     8941 gcgttgttga aaaatcatca gcccatcaca gtagaaagca gaaaggaatt ttatgaaaca
     9001 actaattcta gcggaaaaac caagcgttgc gaaagaccta agcaaggtgc ttggtgctaa
     9061 tcaaaagcac aaaaattatt atgaaggacc caaggtgatc gttacttggg cccttggtca
     9121 tttattagga ttgaaaatgc cggaagatct gaataaggaa tggcagagct ggcaaatgga
     9181 cacgttgcca atgatcccta aaaaactagg aatcaaacca cttcctaaaa caggacatca
     9241 attaaaagca atcaagcagc tggctcagcg aaaagatgtc tcagaagcag tcattgcgac
     9301 agatgccgga agagaagggg aacttgttgc tcgctggatc ttagagtatg tgcatttcaa
     9361 taaaccagtg aagcggttat ggatctcttc tcaaacagat aaagcaatca aaactggatt
     9421 caagcaacta agacctgcga aagaatatga tgcattgtat gaatctgctc ttgcccgggc
     9481 aaaagcggac tggctggtag gattgaatgt gaccagagcc ttaactgtga agtacgcgga
     9541 taatttatca gcaggccgtg tgcagacacc gacactagca ttagtgagag accaagaaaa
     9601 gaaaatcgag cagttccgtc cacaaacgta tttcacgatc acgttgcaag ctggaaaaga
     9661 acaagccaaa atgcttcaaa aaaatcaatt tgctttaaaa acacaagaag aagcacaaaa
     9721 acttgttcaa ttgatcagta caagcaaagg aactgtcaca gaagtccaag aaaaattaaa
     9781 aacggaatct gcgccattgc cttatgattt aacagagatc caaagagaag ccaatcaacg
     9841 ctatggctat tctgcaaaga aaacattaag tcttgtacaa agtttgtatg agacccataa
     9901 gatcgttact tatccgcgta cagacagcaa atatctgaca aatgacatga aaagtacgat
     9961 gaaagaacga ttgcaagcag tcagcgactt tgctcctgag gtcaagcagt atttgaaaaa
    10021 cggagccatc gtccaacaga caaaagtatt ccaagacagt aaagtgaccg atcaccatgc
    10081 acttttgcca actgaaaaca gagcacgtta tgaaaaatta agtaatgaag agcgaaaaat
    10141 ctatcaaatg attgtctctc gttttttagg actgtttgct caaccccata aagtcagcca
    10201 aacaaaagtt acggttgaat ttggtaaaga acgattcatt tttcggcaaa atcgtgtcgt
    10261 ccaagcagga tggaaaggcg aaactgaaac tgagacggaa acagtcaaat gggaaaaagg
    10321 aatgagaatc aatcccgatt tcgccatcaa aaaagaacta tctgcaccgc ctaaaccatt
    10381 gtcagaagca agcttgcttg gtcaaatgga aaaatacagt ctgggaaccc cagctacgcg
    10441 tgcagagatt atcgagaaac taatcaaatc tgaactgatg gaacgtaccc ctcaaggact
    10501 gcaagtatct cctaaaggta agcaattatt agtattggta aatccctctt tagtaacacc
    10561 agatttgaca gaaagctggg aaaaagacct tgaagctatt gctgcaggaa agaaacaagc
    10621 aggaaccttc ttgaatggaa tcgaaaaaga aacaaaacgc ttggtgaatg agatcaaatc
    10681 aagcaaacaa gaatatcaag atttctcgat tacccaaaag aaatgcccag aatgtggggc
    10741 caatctgcgc gagaaaaaca cacgtgacgg caaaatctat gtctgcacca attcagattg
    10801 caactaccgc agaagaaaag atccaaaagt ctctaatcat cgatgcccgc aatgtcaccg
    10861 gaaaatggaa atcatcgaag gtaaaaacgg tgcttatttc cgttgtaaat acgatggaac
    10921 gacagaaaaa ttgcttgata aaaaagagca gaagaagaaa atgaccaaac atgaagaacg
    10981 acgcttgctg aaaaaatatg cacaagcaga agaaccagaa gaaagcccat tagcagcagc
    11041 actaaaagca gcaatgaaga agtaataaga tatgaaagag gttgtgatag aagtggtctg
    11101 cttcaagaaa taagaaggaa tttacgaaaa ttgcttctca aatttttgtg aaatttcgg
//
LOCUS       DAHHZJ010000097        10975 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.098, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000097 DAHHZJ010000000
VERSION     DAHHZJ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.098"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(39..1661)
                     /locus_tag="NBL02_001908"
     CDS             complement(39..1661)
                     /locus_tag="NBL02_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCD2572588.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(1989..3302)
                     /locus_tag="NBL02_001909"
     CDS             complement(1989..3302)
                     /locus_tag="NBL02_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HCD2572589.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(3547..3771)
                     /locus_tag="NBL02_001910"
     CDS             complement(3547..3771)
                     /locus_tag="NBL02_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572590.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(3791..4213)
                     /locus_tag="NBL02_001911"
     CDS             complement(3791..4213)
                     /locus_tag="NBL02_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HCD2572591.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(4355..5065)
                     /locus_tag="NBL02_001912"
     CDS             complement(4355..5065)
                     /locus_tag="NBL02_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572592.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(5083..5580)
                     /locus_tag="NBL02_001913"
     CDS             complement(5083..5580)
                     /locus_tag="NBL02_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD2572593.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(5617..6069)
                     /locus_tag="NBL02_001914"
     CDS             complement(5617..6069)
                     /locus_tag="NBL02_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCD2572594.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(6204..6809)
                     /locus_tag="NBL02_001915"
     CDS             complement(6204..6809)
                     /locus_tag="NBL02_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCD2572595.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(7259..7879)
                     /locus_tag="NBL02_001916"
     CDS             complement(7259..7879)
                     /locus_tag="NBL02_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HCD2572596.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(7881..8750)
                     /locus_tag="NBL02_001917"
     CDS             complement(7881..8750)
                     /locus_tag="NBL02_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HCD2572597.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(8773..9180)
                     /locus_tag="NBL02_001918"
     CDS             complement(8773..9180)
                     /locus_tag="NBL02_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCD2572598.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(9199..10488)
                     /locus_tag="NBL02_001919"
     CDS             complement(9199..10488)
                     /locus_tag="NBL02_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCD2572599.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            10546..>10975
                     /locus_tag="NBL02_001920"
     CDS             10546..>10975
                     /locus_tag="NBL02_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCD2572600.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT         3173 a         2323 c         1829 g         3650 t
ORIGIN      
        1 ccgaaagaaa acactcgtta accatctaag caaacgattt attttacttc caacacaaat
       61 gcttcataag gtcgtaaatc aactttgccg tctggtaact gtttcagagg ataatttgac
      121 acaataaccc ttaaagcttc acgtggaata gtgaacgtct gcttttcact ggacatatta
      181 caaataacga gccattgctg gtcttcatat atgcgagaat aggcaaagac atgctcaggc
      241 gtcttttcca ctaattcata gtctccccag acgatgatgt catgttttct acgcaaatcg
      301 atcaatttct tgtacatata aaaaacagaa tcttgatctg ctaacgcttc ttctgcattg
      361 atttctttat aatttggatt gactcgaagc catggaattc cagtagtaaa cccagcatgt
      421 tcacgggcgt tccattgcat aggcgtccta gcattgtctc ttcctttcgc cttgattgat
      481 tccaagattt cctcttttgt aaatccagaa gaaatccttt cgttatacat attgattgtc
      541 tcaatatctt ttgcttccgc gatatcatcg attgggcaat tcgtcatgcc taactcttct
      601 ccttgataga tataaggggt gcctttcatc atatggagca agatagcaaa catcttggca
      661 ctttcttttc gatacacatc gtcatttccc caacgagaaa caattcgcgg aagatcgtga
      721 ttattccaaa ataaactatt ccatccttca tcacctaaag atgtttgcca tttggataat
      781 acttttttca gttcagagat actcaagggt ttcaaatccc atttgtcttt gccttcttgc
      841 tgatctaatc ctatgtgttc gaattggaag accatggata attcatttct tttaggattg
      901 gaataaagtt tagcgatttc ggtagttgct ccccatgttt cgccaacagt catcagattt
      961 ttgtcaccaa aagttgcttg attcatttcc tgaagatact catgcagttt aggtccatta
     1021 cttgtgattt ctttatccgg ttctttgcca atcatatcga tcacgtccat acgaaagccc
     1081 ccaatacctt tatcgatcca gaaattcatc atttgataaa tctcttggcg aacctgttta
     1141 tttttccaat tcaaatcagg ttgacgcttg ctatacaagt gcagaaaata ttgccccgtt
     1201 ttttcatcca actgccaagc agaaccactg aatattgagc gcaatctatt aggctctttt
     1261 ccattgaccg gatcacgcca aatataatag tcccgataag gattttcttt atttttacgg
     1321 gcctcaatga accacggatg ttcatcagaa gtatgattca ccaccagatc catgatgata
     1381 cggatatttc ttttctttgc ttcttcaatc agttcttcca tttcttccat cgtaccgaat
     1441 tctgccatga ttgcttgata attactgata tcatacccat tgtcatcatt cggagactcg
     1501 tatactggag aaagccaaat tgcatcaata cctaaatacg ccagataatc caacctggaa
     1561 ataatccccc gcaaatctcc aataccatct tgatttgaat cttgaaaact tcttggatag
     1621 atttgataaa caactgaatg gtgccaccat ttttgttcca tgattttcct cctataaatg
     1681 gatgtttcta ttttttgctt aatcatttat tttagtctct ctcactaaaa attgtaacgc
     1741 tttcaaatag ctattgcacc ttgttatcgg taacagaata aaacaaaaaa acagctgagg
     1801 aaattccctc aactgttttt tgaaaggatt gatttatgca gggaagaatg ctgatttcac
     1861 aacttcttta taaacggtgt tcactcagct gtcccttggt attagctcaa aaaacggcaa
     1921 aatataagaa actatttttt cgtttttttc tctaatactc aggccagaac gctgatttca
     1981 caacctcttt atttcacatt caatatccac atgaatccca caaatacaaa gaacagacca
     2041 tacatgattg gagatacttc ttttcctctt ttagctgcta acatggtaat tggatagaag
     2101 ataaaaccta atgcaatccc atctgaaata ctataagtca aaggcatgcc gatcaagatc
     2161 aagaatgaag ggatcgcaat ctctaagttt ccccattcga tttgtcgcaa agattgtgcc
     2221 atcaatactc ctaccataat caatgctggt gcagttactt gtgaagtaac aactgatagt
     2281 aatggagaaa agaacaagcc taacaagaag aaaatccctg tagtaatagc tgtgatacct
     2341 gaacgtcctc caacggcgat accagctgat gattctacaa atgcaccaac aggagaagta
     2401 ccaaataagg aacccgcaag catagctgtt gaatcagaag ctaaagcttt ccctacacga
     2461 ggcatcttgt tgtctttcat gaaacctgct tggtttgcta atccaactaa agttcctgct
     2521 gtatcaaaga aagtcacaag caagaatgtc agcactacga cccacatttg aagagaattg
     2581 atgtccccta catgttttaa tgcaactaaa aatgttggtt tcaaacttgg tgctgcggca
     2641 acaatttcag aaggcattgg aatcaaacct gtgaccagac ctaaaatcgt tgtagctgcc
     2701 atcccgatga agatgccacc tggtacacgc cgaacaagca agatagcagt tacgactaat
     2761 ccaaaaatcg tcaaccaagt agaaccaacg ttgagtgatc ctaatcctac taatgtagat
     2821 tcattcgaaa cgatgatccc accttcgctc agaccaagga aagcgataaa tagaccgata
     2881 ccgccagaaa tcgcatattt caaatctgct gggatcgcat caatgatcat ttcccgcagc
     2941 ttgaagattg tgatcaaaat aaaaatcaaa gaagcaacga atacgccggc taatgctgtt
     3001 tcccatggaa tccccattcc taaacaaaca gaataagcaa aaaaagcatt gatcccaaga
     3061 gcaggtgccg ttccaattgg ataacgggct aataccccca ttaaaacaca gccgatcgca
     3121 ctggctaatg cagtcgcagt aaacactgct ccttcatcca ttccagaagc acctaataca
     3181 gttggattta caaataagat ataagccatt gagataaatg ttgtaaatcc tgccagtacc
     3241 tcacgtttta ccgtggtgtt caatttttct atttcgaagt aggaaagtat tttttccttc
     3301 atcttccatc cccctaaatc cgaacattat aaataaagtc cgttaaaatt tcgatttatt
     3361 aataaaacaa cgtgttttat catatactaa aacaaaacat aagtaaacaa aaaattcgga
     3421 ttttaagcaa aatccgaacg ttccatctga ttaaatccct ttatctcaaa gttcatgaac
     3481 attcactttg gattacaaga atttctttat tgtgttaata aagtttcttc attctagtct
     3541 caaaaatcaa tagtgtgatt tgattttacc atttcgtttc tgttcttctt tgatgaattc
     3601 ttttgtatcg acaggattca gattatttag tccgccaaaa cctgtatcga ccatttttcc
     3661 ccagcctttt gtcttctttt ttcctgattt gagattcttt gctcgaagct tctcactatg
     3721 ttttgctcgc cgatacttca aataaccttc taatcttccc attagatcca ctgtttttcc
     3781 tcctttttat tcaatcagac gactcagctt ttcctataac aaaataattg ccttcagggt
     3841 cagaaaaatt gaaagtcaag atgccattgt tatctgtgat ttctcctggt tcttccatgt
     3901 tctcgtagat agtttcaaat tcatccgtaa aaaatactaa agaaggcggt ggtcctaata
     3961 cctctggtga aaacttctgc acaaatactt ttgggaatac gcctaattct aattcttttg
     4021 agatgctgag tacaatactc ttaaattctt ctggcaattc gattgtttca gaaatttctg
     4081 caccaatttt ttctacaaag aatcggcaaa tcatctctac gtcattgaca taaagcatca
     4141 cacgtgtttt ttgtatcatc atttgaccct tctttctatt tgattttctt tcctgtctat
     4201 aaactgattc cattatatca cgaaagcgca aacaccttgt gtttgatact attcaagaaa
     4261 aataaagcaa tagtttctag gattgctttc ctccacaaac tttttttaca aaaaaaaaac
     4321 ggcaatgaat gccgctcttc ttatttcgat tcttttataa ttctgcttct gattcatctt
     4381 tggttagtgt gtgtctaaca ctagttacgc caaatgtttg atcgattttt tccttcaacg
     4441 cttcgattgc ctgtgcgcca cgtgccgctt gttcagcatt gatggattca atgaccagca
     4501 ccgctgcttg tgtttcttcc gtcagcatcg ttctttgggc ttcacgccag ttgagacagc
     4561 gacagatcgc tccttgttca tcatagtaga tgatttcttc cggaagtgcc ggagcatcgg
     4621 aatctgcacc taaaggcagg aatgattcgc caccttgggc ttttcctaaa tgcagatttc
     4681 ctgcgatctt ttcgatatct tctccgccgc aaggtacgcc aaaagacaag gaaatactat
     4741 tatagatatc tacaagcgga ttgattggga aaaattcccg ttcttgattt gcacgtttca
     4801 gtaaagcttc aatagaagaa cgtgcacctt ttttagtctt gaatgtggta aatgcctgac
     4861 gccactgagc aatgacttcg ttttggctga atgtttcttc tgtaaggtaa tttcttgctt
     4921 cttttgctgc ctcattcaat aattgatgat acgtttcttc actttctttt gttacgtggt
     4981 tatcgatacc atttacggat aagatttcga tggttgcttc aggaaatact tcccagaaat
     5041 tggtatccac tattactttt ttcatctgtt cacacctctt gattagaata attgcttgat
     5101 gaatagatag tctgtttggg gatcatctcc catataaaaa acatgttcgc tgtatttgat
     5161 aaatcctttt ttacgataaa aagcgagcgc aacatcgttg tgttcccaaa caccgagcca
     5221 aatggctgtt ttttgctttt cttttgcttt ttcaatagct gtatctagta aaaatgtacc
     5281 gataccttga tgtttaaatt tttttcgtac atagattcgc tcgatttcta aaaaatgatc
     5341 agccatttcc tcagattgac tcgttcctgt atttagtttc aggtaacccg caggctcttg
     5401 atcgatactc actaggaaaa aatcggtttc tttatctttt agctcttctg taagtttttc
     5461 tagtgcataa gcttgtgtca agtaatcagc taaatgctcc ggtgaattat cttttccaaa
     5521 tgtatctgta aatgtttcga tactaatttg ttgtagtaag tctacatctg aaggcgtcat
     5581 ttttcgaatt tcgtatttat tcacggcttc ttcctttcaa tagatccttt tattgccttt
     5641 tttgacaaaa ttccaatctt cgctaacatt cttgcatact ttatttaaaa gatattccaa
     5701 ttgttttgct tcttcgtcag aaagaccatt taatgcgact gcattggagt agttgttttc
     5761 tcgaatgatg aaaggataga tttctgctcc ttttttagtc ggatagagtt tcttgatttt
     5821 tttattttct ttatcttcca aacgttcgat catcccattg gattctaatt tcttgattgc
     5881 tcgggcagtc gttgtacgat ctactttgat catttctgct agtttttctt ggatgattcc
     5941 aggattttcg caaatacgta ctaaataaag atactgtcct cttgttaaat cgacttcttt
     6001 gaattcgata ttgctaatag aatctagcgc tcgggcaatc attccgatgt ctcgaaggat
     6061 ttcagccatt tcatcccacc tttattttgt ttgtaagact agttcacctc tttttgttgc
     6121 aaatgcaact attttcaata aaaaaatcaa gttctttttt aataactagt aaagtatatt
     6181 cttttcttct tttacgaaaa agctcaatca caatagatct ggtttttgtt ttctaaaata
     6241 agtaactgtt tcttcgttgt tcgcttttct gatagctttg gcaactcttc tatactaaaa
     6301 aatcccatgt tggaagtttc attattctca ataaacttgg cctcttcacc gtggatcgca
     6361 caagcaaaaa caagtttata atactgaaaa agctgtggaa tatctttttg tttattcgta
     6421 tcaaaaactg ctcgcaactg agtagtttct actgttaatc ctgtttcttc atagacttct
     6481 ttacgcacat tttcttcagg agacaatcct atttcagcaa atccaccagg aagtgaccat
     6541 tctttcgtcc gtttatcttc gattagtagg accttgccat cttttttgat ataggcacga
     6601 acatctactt ttggtgttgg atgtccttct ccaatcgttg tgagcgcttt tatttcttcg
     6661 aaagtttctt ttcctatttc actgatcagt tccaatgaaa tcgtccggat ctcctcaaaa
     6721 cgctctttat caaatacatc ttttccataa tatagaccag cttgagaaag tgcgagtagt
     6781 cttttgtatt gagccaactt cttgttcatt ctcttccctt cttttttagc aaatgtatga
     6841 ctatgtaatc aagtatagaa gactggttta attacggcaa ataaatataa tgaaatctgc
     6901 aataaataaa agaatgttaa aaaactcgag aacaaaagac attcgacttc tctggggctg
     6961 tgaataaatg gcgttcaaaa gcttgcatct gcggtaataa gccccaccaa acgaaaaata
     7021 tgacgagcta ttcttgtctg gctgttctta ttaattgagg caggacagct ttttcacaac
     7081 ctccccacta aacggtgttc tcctaccaac tcctcggtca caagtcacaa tattcatcaa
     7141 acatgagaag ctgttttttt gaaattgctc cttgtgactc ggagctgaac gacaggctca
     7201 cgacctctcg gtaaacagcg ttcaaaagcc aatttttcga tattaagcca tttctttgtt
     7261 acttattgtc attgatttct ttttctaatt tatccaatag tggagtgtaa tccacttgag
     7321 tatagtctgt tacatcaaat tcatatagtg ttcctaactg gaatttgtca tattttcggt
     7381 cggcgatgat ctttttgaat gcttctttcg taatatgatt ggtccctttc gttcgatctt
     7441 ccaatacgtc accatagtga taatgatcca tgatatagct caaatgacga tctgattcac
     7501 gatcacgttt gtaccgacgt tgccataaca cttcaaagtc tgctgttagg cgaatcgtga
     7561 tcacctgata tccgtatcta tctgctaatt cttggagtcg tgctttttgc ttatcactaa
     7621 aaggatactc ggacacaatc actttctttc ctgcttccat atacaaatca agtgctttgt
     7681 aatagaaagg ataaactttc gtcctttctt gttccgcccg ttcttccaaa ttgttaaacc
     7741 cgatggattc tgcatagtat tccttgattt catcgagcgt gatggcatac atatctggaa
     7801 atcgttcccg tagtttattc ataagatatg tttttcctgt accaggacta ccagccagaa
     7861 gaatgaaata ctttttcatc tcatttattt cctaagttaa ttggactttc caagttcata
     7921 aacagtccaa tcttatgctt gacaatttct tttactgctt catttgctgg aatcacatta
     7981 tggcgaagag acttgttgat ttctgtttca ctaaaggttt ctttagctac tttcgtccag
     8041 ttgactaaaa gctccgttcc aacattgaat ttgcggatcc cgttattgat caattcagga
     8101 taatcttctt ctttcacacc tgttcctcca tggataacta gggggacatc aactactgca
     8161 tcgatttctt tcagcaaggg aatgtttact tctgtttttg atttgaattg tccatgattc
     8221 gtaccgatag caatagctaa tgcatccacg cctgtagctt ctacaaattt cactgcgtct
     8281 tccggtcttg tatatacctt gtcattttca gcgacatgga ttccttcttc tgtaccgccg
     8341 attgttccaa gttctccttc tacggaaaca ccgtgcgcat gggcatattc tactactgct
     8401 tttgtcttca ggatattttc tttgaaaggc agatgggacc catcatacat gacagaagta
     8461 tatccgctgt cgattgcttc tttgatatca tcaaagtctc gggcgtggtc taagtgcaag
     8521 actgcatcaa ccatttcttc ttctgcaata gctgtcatta cattgactaa cacatgcatg
     8581 ccgatatatt tagcggtatc gacacttgtt tgaataatga tcggcgaacc catttctttt
     8641 gctgcgccta gcatagcagg aagcatttct aggttatgcg tattaaaagc tccaactgta
     8701 aaattcaact cttctgctgt ttttgttact tctttcaatg ttgtatacat cttttattgc
     8761 caccttatct ttttatttgt ttcctttgat cgttacttgt tttgcaattt gtcgcatctc
     8821 atccattttg cccatgtagt aatcgatccc ttgatcatca ccagcacgag cagcatctgc
     8881 acgcttctgg ttatagaggc tcatcagttt tttataccca tctccgatcg tctgtttatt
     8941 ttccaaactc ttttctaaat attggatggc ttgatcgttc acatacaatt cagaaaattt
     9001 aagtccaact tgttcataaa gtttcgctaa tttatgctta tcttgggtag tggaaatctc
     9061 ttgtttcaat tgtgtaatct ctgattgaat cttttcggtc gcttctggcg atagggcttt
     9121 ttcttctttt gccagaactt ttttcggttc tttttctttt ttcttaaaaa atccaaacat
     9181 cattttccct actttcaatt aaaatgcttt caaaactggg cttaagatcc aggcatataa
     9241 gagtgctgcg gctactgtat ctacatcggt tgctgtagca ttgttgaatc ccatcgaatt
     9301 aaagatcgtt actagcaaag cgggcaataa tgtaataaag aaaccatgag caataccgcc
     9361 aatgatcgct cctcggcgtc cgccaactgc gttgccaaat atccctgctg tccctccggc
     9421 aaagaaattg gttagcatac caggtaaaat catcgctaag ccgaacatgg gcaaagtaaa
     9481 catggcaata atcgtaccaa tcgttgtcgt aatgaaacct aaaatgacag catttgggct
     9541 ataaggaaag aaaactggac aatctaaggc agggatcgca tcaggaacta atttcatagc
     9601 tatccctcga aatgccggca cgatttcacc taacagtaga cgtacaccag ctagcaatac
     9661 atacacaccc actacgaatt ggatggcttg taaaaaggca aacatgatat agttttgatc
     9721 tcctgataat tcagaagcga atggttcgcc tgcaaataaa acagtgatga tatatagcgg
     9781 gaccatcacg accatgacag acaaataagt atcttgcaga aattcgaaat gtgtcggcaa
     9841 cttgatatct tcgatcgact ttttattttc gcctttctca ccaaaaagct tagcaacacc
     9901 ggcttcaaat aagtacccga tcgtacaaaa atgacctaaa gcaatatcat ttgatccagt
     9961 gattttacga atgatcggtt gcgctactgc cggcatagca atggcgaaac aagctccgat
    10021 aaaaccaccg accaagatca atacgatccc tcgcagaccg gcaaaatagc caaagactgt
    10081 cgtcatcgtt gccatccata aaattgcttg accagttaaa aaaatgtact tccattttgt
    10141 gaaacgagcc aaaataatat tgaatacaaa aatggctaag aacgtcagtg caatatctct
    10201 acctaaaccc aaatcattca ttgcttgacc attgatagat tcaatagaag ggatgatccc
    10261 ttgcatatga aatccttcag taaagatttt cccgaaataa gttaaacttc caacgatgat
    10321 gctcgaaccg gcactcaaca cttgaaatcc taggagtgtc tttaacgtgc cagagatgac
    10381 ttgtcctgct gatttcttct gcagtagtag acccaacata gcaatcaatg caatggtgat
    10441 cgatgcctga gtcaaaatat tttcgatgat aaaattgata acgcccatgt tttgatgtgc
    10501 accccaaaag ttagactaga aatctaatta aaaggggtgc tttttttgcg gaaatataca
    10561 tttgttttta agaaaaaagt agtttcagac tatttaaata acgaaggcgg ctacaaatat
    10621 cttgcacata aatatcaaat aaatcgtaca ctggttagac attgggtaag gatttataac
    10681 tatcatggtt gggaaggctt ggttggaggt ggcaaaagct acactacaaa atttaaactt
    10741 gatgttatag aatatatgga aacaaatggt ctttctatcc aagaaactgc taaaaaattt
    10801 aatatcggtt caaatagaac tctaagtaaa tggatagagc aatatgaaga aggcggtgct
    10861 tcttcacttg agagccaaaa aaggggcaga aaaattagta tgaattccaa gctaaacatt
    10921 cctaaaaaac ttaaagatga gtctcttgaa gaagaagtta ttcgtttaag agcag
//
LOCUS       DAHHZJ010000098        10972 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.099, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000098 DAHHZJ010000000
VERSION     DAHHZJ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.099"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            31..759
                     /locus_tag="NBL02_001921"
     CDS             31..759
                     /locus_tag="NBL02_001921"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCD2572601.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            759..1013
                     /gene="purS"
                     /locus_tag="NBL02_001922"
     CDS             759..1013
                     /gene="purS"
                     /locus_tag="NBL02_001922"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HCD2572602.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1015..1686
                     /gene="purQ"
                     /locus_tag="NBL02_001923"
     CDS             1015..1686
                     /gene="purQ"
                     /locus_tag="NBL02_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HCD2572603.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            1687..3909
                     /gene="purL"
                     /locus_tag="NBL02_001924"
     CDS             1687..3909
                     /gene="purL"
                     /locus_tag="NBL02_001924"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HCD2572604.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            3894..5333
                     /locus_tag="NBL02_001925"
     CDS             3894..5333
                     /locus_tag="NBL02_001925"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCD2572605.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            5356..6408
                     /locus_tag="NBL02_001926"
     CDS             5356..6408
                     /locus_tag="NBL02_001926"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCD2572606.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            6405..6983
                     /gene="purN"
                     /locus_tag="NBL02_001927"
     CDS             6405..6983
                     /gene="purN"
                     /locus_tag="NBL02_001927"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCD2572607.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            6980..8515
                     /gene="purH"
                     /locus_tag="NBL02_001928"
     CDS             6980..8515
                     /gene="purH"
                     /locus_tag="NBL02_001928"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCD2572608.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            8531..9805
                     /gene="purD"
                     /locus_tag="NBL02_001929"
     CDS             8531..9805
                     /gene="purD"
                     /locus_tag="NBL02_001929"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCD2572609.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            9927..10841
                     /locus_tag="NBL02_001930"
     CDS             9927..10841
                     /locus_tag="NBL02_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCD2572610.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
BASE COUNT         3606 a         1855 c         2618 g         2893 t
ORIGIN      
        1 ttttaatgta aataaaaagg agagacgtac atggagaaga aagaactttt gtatgaagga
       61 aaagccaaaa aaatatatct tacttctgat gagaacttga tttggataga atatttagat
      121 caagcaactg cgttgaatgg cgcgaggaaa gaccagataa gtggaaaagg agaattgaat
      181 aatcaaatca caagtgcaat tttccgttat ctgactcaaa gaggaataaa aaatcatttt
      241 attgaacaat tgtctaaaac cgaacaatta gcaaaaaaag tacaaattat ccctctggaa
      301 gtggttatcc gaaatattgc agcaggaagt ttctcaaaac gtctaggtgt tcccgaaggt
      361 aaagaattca tcaaaccaat cattgaattc tactataagg atgaccagtt ggatgatcca
      421 tttatcaatg aagagcatat ctatttctta caacttgcaa agccggaaga aatcagtgag
      481 ctgaaagccc aagcgttggc tattaatgaa attttgaccg aattatttgc taaggtcggt
      541 ttggttttag tagatttcaa gctagaattt ggccggtcga atggacaaat tttgcttgcg
      601 gatgaagtaa caccggatac ttgccgatta tgggatgaac gtacgaatga acatatggac
      661 aaagacgttt accgcagaga tctaggtgat ttgatcccag tctatcaaga agtatataac
      721 cgtctatcgg aactaactga agaaaaggag cgagtatgat gtatttcgta aaagtatatg
      781 taacctataa agattcagtg ttagatcctc aaggggaggc agtaaaaaaa gcagtccatc
      841 gtatgggata tgaaacaatc gaagatatcc gaataggaaa atactttgag atacaacttg
      901 ctgatgcaga agaaccaatc gatacagtga ttgaaacgat ttgtgacaag ctgcttgcaa
      961 atgtaaatat ggaaacatac cgatatgaaa tcaaaaaaat ggaggaagta taacatgaaa
     1021 tttgcggtca tcgtttttcc aggttcaaat tgtgacgcag atatgttatg ggcagttcgc
     1081 gagatcatgg gcgctgatgc cgaatttgta cggcatgatg agtcctcttt agaaggattt
     1141 gatggggttt tactgccggg aggattctcc tatggtgact atttgagatg cggtgcgatt
     1201 gctcggtttt ctacaatcat ggaagaagtg attcgttttg ctgaagaagg gaaaccagta
     1261 ttcggtacat gcaatggatt ccagatattg acagaagcag gacttttgcc aggtgtactg
     1321 agacgaaatg agtctttgca ttttatttgc aaaactgtac cattgaaagt caaccccaag
     1381 acggccttta cttcagagta tgaagccgac gaaataatct atcttccagt tgctcatggg
     1441 gaaggaaatt atttctgtga cgaagaaaca cttcaagaac taaaaaaaaa taatcagatt
     1501 gcctttactt ataatgacaa catcaacggc agcgtggaaa acattgcagg gattacgaac
     1561 aaagcaggga atgtgttagg aatgatgcct catccagaac gtgcagtaga agacttgcta
     1621 ggatcagaag atgggaaacg gttctttgct tccattttaa aaaacttcgg aaaggtgacc
     1681 gtatagatga aaacattgaa agaaccgaca gctgaagaga tcaagaaaaa aagaatctat
     1741 gccgaatggg gattgactga tgaagaatat cgaatgatcg aagaagatat cttaggaaga
     1801 atgcctaatt atacggaaac tggactgttt tctgttatgt ggagcgaaca ttgttcctat
     1861 aaaaattcta aaccggttct acgcaaattt cctactagtg gacctcatgt gttgcaaggc
     1921 cctggagaag gcgcaggcat cgtcgacatc ggtgatggac tagcagttgt cttcaaagca
     1981 gaaagtcaca atcatccttc agccgtggag ccttatgaag gagctgcgac aggagttggc
     2041 ggaatcatca gagatatttt cagcatgggg gctcgtccaa ttgcgttgct agattcattg
     2101 aggttcggcg aattagataa tccaagaacg aaatatttac tagaagaagt agttgcaggt
     2161 atcggcggat acggcaattg tatcggcata ccgactgtag gcggcgaaat tgcatttgat
     2221 ccttgttatg aaggaaaccc attagtcaat gcaatgtgcg taggtttgat tgaacacaaa
     2281 catatccaga aagggcaagc cgctggcgtc ggtaattcga tcatgtatgt aggtgcaaaa
     2341 acaggtcgcg acggtatcca cggagcaact tttgcctcag aagaatttaa tcaagaagaa
     2401 gaacagcaac gttctgctgt acaagtaggc gatcctttta tggaaaagct gctgcttgaa
     2461 gcgtgtttgg aattaatctt gcatcattcg gattctctaa taggtataca ggatatggga
     2521 gcagcaggac ttgtttcatc tagtgcggaa atggcttcaa aagctggctc aggacttata
     2581 ttgacattag atgaagtccc acaaagagaa actgggatga caccttatga aatgatgtta
     2641 tcggaatcac aagaacggat gttgatttgt gttaaatcag gagaggaaga aaaaatccaa
     2701 gaactatttc agaagtatga tctagatgca gtcactatcg gtaaagtaac agacgacggg
     2761 caatatcggc tatatcatca aggagaagaa gtagcaaatc tgccagtcga tgcattagct
     2821 gaagatgcac cagtttatca taaagaaatg aaagaaccag ctcgaatcgt agaatttcag
     2881 cagttagctc cttatcagcc agttatcgaa gaacctggac aagtattgct tgatctgctg
     2941 cagcaaccga cgattgcttc aaaacgttca atctacgaaa cttatgattc acaagtacaa
     3001 accaatacag ttgtccgtcc aggaagcgat gctgctgtga tgagggttag agggacgaat
     3061 aaagcgttag ctatgaccac agactgcaac gcgcgctatc tgtatttgga tccaaaaatc
     3121 ggaggacaaa tagcagtggc agaagctgct agaaatatta ttgctagcgg tggtaagccc
     3181 ttggcgatca ccgactgcct gaactatggt tctcctgata aaccagaagt tttttgggaa
     3241 ttatcgactt cagctgacgg aattgctgct gcgtgtgaaa cgttaggaac tcctgttatt
     3301 tcaggaaatg tctctttata taatgaaacg gatggtcaag caatctatcc aacaccaatg
     3361 attggtatgg tcggattgat tgaggatcat aaacatatca ctactcagga attcaaaaaa
     3421 tcaggggatc taatctatat attaggtaag acgtttgcag attttgatgg cagtgaatta
     3481 caaaagatgc agcttggaag aatcgaaggt gtgatccgaa actttgatct atccattgaa
     3541 aagagaaacc aagagttagt attgacagcc atccaaaacg gattgattga aagtgctcat
     3601 gactgttcag aaggtggctt ggcaattgca ctggcagaat cagctttcaa acatcagcta
     3661 gggatatctg tacagtttga attgtcctct gctcaattat ttgcagaaac tcaatcaaga
     3721 tttgtgttga cagtcgcacc ggaaaataag acacgattcg aagaaatgat gggagatgct
     3781 gctgttttag caggtaaggt gacggatgaa gcgataatcg aaatttcagc aactgacggt
     3841 cagatcaaaa tagaaactgc agtagcaaga aagtgctggg aggatgcaat cgtatgtctt
     3901 atgaagtgaa gagtttaaat gaagaatgcg ggatcttcgg tgtttggggt catccagacg
     3961 ccgctagtgt ggcatacttc gggctccata gtcttcagca tcgaggacaa gaaggtgcag
     4021 gtatcgtgtc caatgatcga ggaaagctta aggggcatcg agatttgggg ctgctttctg
     4081 aggtattcaa agatcaaaga aaattgcagc agttaacagg agaagctgca atcggacatg
     4141 tacgttatgc aacagcaggc aatggaagtg tggacaatat tcagcctttt ctattcaaat
     4201 ttcatgatca gtcgatcggc ttagcgcata atggtaactt gatcaatgct cgttccctta
     4261 gaaaagagtt agaggaaaat ggggcgatct tccattctaa ctccgatacg gagatattga
     4321 tgcatttgat ccgacgaagt gaagaaacag attttttaaa taaattaaaa agtgctttga
     4381 atgaagtcaa aggtggcttt gcctatttat taatgacaga aacggcaatg attgccgctt
     4441 tggacccaaa tggttttcgt ccattagcaa tcggacaaat gaaaaacgga gcatacgtga
     4501 ttgcctcgga aacttgtgca ttagaagtta ttggggcacg ttttataaga gatgtccagc
     4561 ctggtgagat agtgatcatc aatgacgaag gaatccagat tgattgcttt acgaaggaca
     4621 cgcagctttc tatttgttcg atggaatata tctattttgc cagaccagat tcaaacatcg
     4681 cagggatcaa tgtgcatact gcacggaaaa atatgggacg gaatttagcg aaagaatcgc
     4741 cagtaaaagc agatatggtc gttggcgtac ctaattcttc tttgtcagcc gcaagcggtt
     4801 atgcggaagc tagtggaatc ccttatgaaa tcggattagt aaaaaatcaa tacattgccc
     4861 gaacatttat ccagccaacg cctgagctaa gagagcaagg tgttcgaatg aaattatctg
     4921 ctgtacgagg agtagtggaa ggtaaaaagg tgattatggt cgatgattca attgttcggg
     4981 gtacgacaag cagacggatc gtccatcttt tgaaagaagc aggggcaaaa gaagtccatg
     5041 tgaggattgc ttctcctcct ttgaaatatc catgttttta cgggattgat attcaaacgc
     5101 gtaaagaact cattgcggca aatcacacga ttgatgaaat caaagaatgt atcggtgcag
     5161 attctttagg ttttttaagt gaagagggtc tgatcgaatc gatcggtctg gaaaaagatg
     5221 ctccgtattc aggattgtgt atggcttatt tcaacggaga ttatccgacg ccgttatatg
     5281 actatgaaga aaaatatcag gaatcactaa aagaaaaagt atcgttcttt taaaagcaga
     5341 ccaagaaggg agtatatgaa ttcaatggca aatgcatacg caaaagctgg cgtagatgtc
     5401 gaagctggat atgaagtagt agaacggatc aagaagcatg tcaaaaaaac ggaacgttta
     5461 ggtatgatgg gcgctatagg aggattcggc ggttgttttg atttatcggt ctttcaatta
     5521 aaagagccag tccttgtctc ggggaccgat ggcgtgggga cgaaattagc agtggcaatc
     5581 gaagcagaca aacacgatac aatcgggatc gactgcgtag cgatgtgtgt caatgatatc
     5641 ttagcacaag gcgcagagcc gttatatttc ttagactact tagcaacggg gaaaaatgat
     5701 ccgttacgtt tggaaaatgt agtaaaaggt gtagcagatg gatgtgttca aggaaatatg
     5761 gcgctgattg gcggagaaac agctgaaatg cctggtatgt actcaggtaa tgattatgat
     5821 ctagccggat ttgctgtagg gatagctgaa aaatctcaat tattgacagg agaaacgatc
     5881 aacccaggag atgtgttgct tggaatagca tctagcggga ttcattcaaa tgggtactct
     5941 cttgttcgca aaattttctt tgagcagcag cagttttctt tatcatcaaa actgccagaa
     6001 ttgaaaaaaa cacttggcga agaattgctt gagccaacga aaatctatgt ttcttccttg
     6061 cttccgctga tcaaagaaaa gaaaataaaa ggagctgcac atatcactgg tggagggttt
     6121 gtagaaaata tcccgcgaat gttaccagaa tctatggctg cacaaatcaa acttggctca
     6181 tggccgattc ttccaatatt cggctgttta caaaaatatg gtgaaattcc tgagatggag
     6241 atgtatgaaa tcttcaatat gggcatcggt atggtcctgg cagtcgaaga atcgaaagta
     6301 aaagaaatag tacacgaaat tgagcaagct ggcgaaaaag tttatgaaat cgggaaagtg
     6361 atcgccaaag aaaaacatcg tgtggtgttt ataggagaag aacgatgaga atcgctgttt
     6421 ttgcttctgg aaacggaagc aactttcaag cgctagccga ttatctatcg aaaaaaggat
     6481 tggaatcttc gattgactgg ttgttttgtg atcagcctga agcatatgtt ttgaaacgtg
     6541 caactgcgtt gagtgtaccg gcagattgtt tttcacctaa agaatttgat tcaaaaaaag
     6601 agtatgaaga agcaattttg cataaattga aagaaaaaaa gatcgatttg atcgtattag
     6661 ctggatatat gcgaatcatt ggaccggtat tgctgaaaaa ttatgataaa agaatcatca
     6721 atatccatcc gtctctttta cctgccttcc ctggtttgca tggcatacgt gatgcctttg
     6781 aagctggagt aaaagaaacg ggtgtcacaa ttcattatat cgaccaaggt gtagatactg
     6841 gaccaatcat ccggcaggaa aaagtgagaa ttgaacagga ggacacgttc gattctttag
     6901 aagaaaaaat ccatagagta gagcatcgaa tctatccaga agtgattagt gaaattattg
     6961 aaaatggagg ctatctgaaa tgacaagagc attgatcagt gtatcagata aaactgggat
     7021 tgtagcattc gcccaaggtt tgattgaaca aggagtagaa atcatttcaa caggaggaac
     7081 aaagcatgcg ttggataaag ctgggatcaa aacgctttcg atcgaagaag tgacagggtt
     7141 tcctgaaatg atggatggcc gtgtcaaaac acttcatcct aaaatccatg gtggtctttt
     7201 aggaagacgc gatctcccta gccatatgga ggcaatggaa acggaaaata tccaaccaat
     7261 cgactttgtt tgtgtcaatt tatatccttt caaagaaacg attatgaaac cagatgtcga
     7321 aatagcagaa gctattgaaa acattgatat cggtggtcct agtatgctcc gtagtgcagc
     7381 aaaaaaccat gaatttgtca cctctgttgt tgatccatct gactatgaag aagtactttc
     7441 agaattacgt cagtatgggc atacttcact ttctttcaga caaaaactag cggcaaaagt
     7501 ctttcgtcat actgcggcat atgatgcact gatcgctcaa tacttaacgg attgggtgga
     7561 agaaaaagaa ccggaaaaat tgacacttac gtatgaacga aaacaaacgc ttcgttacgg
     7621 tgaaaatagc catcaagccg ctgcctttta tcagtccgct cttctggaaa aatattcgat
     7681 cgcagcagca aaacaactcc atgggaaaga actttcttat aataatatcc gagatgcaga
     7741 tgctgcgcta cggatcatga aggaatatga cgagccaacg gtagtcgcct tgaaacacat
     7801 gaatccttgt ggtattggga cagcatctac gattttagaa gcgtgggaag cagcgtatga
     7861 agcagatcct gtttcaatct ttggtgggat catcgttttg aatcgagaag tagatctgcc
     7921 aacagcagaa cagatgcata agctgtttct agaaatcatc attgcccctt cttatacaca
     7981 agaagctttg gaattactgc aaaaaaagaa aaacatccga ttactgacag tagactttgc
     8041 gcaggaagaa aatcaaaatc gagaagaaac ggtctcagtc ttaggtggac ttcttgtaca
     8101 agatcaagat ctggcaatag aaaatgaaga agagtggaaa gtcgtgacaa aacgtaagcc
     8161 gactccagca gaagcaaaag cattagtgtt tgcttggaga gctgtcaaac atgtgaaatc
     8221 taatgcgatc gtactggcaa acgaacgtca gacagtagga atcggagccg ggcagatgaa
     8281 tcgagtcggt tcagtaaaaa ttgcgattga acaagcgcaa gagaaaatgg aaggtgcagt
     8341 tttagctagc gatgcttatt ttccaatgga tgattcggtt gaatatgcag ccaaacatgg
     8401 tataaaagca atcattcagc caggaggaag catcaaagac caagattcta ttgatatggc
     8461 gaataaatac gatctagcaa tggtctttac tgatgttcgg cattttagac attagagagg
     8521 agtcggataa atgaacattt tggtgatcgg tgcaggtggt cgtgaacatg caataagtaa
     8581 aaaactttta gccagtccga aggtggatac ggtgtattgt gcgccaggaa atccggggat
     8641 gacaaaagat ggtatcaatt gtgtcgctat cagtgagcga gatcattcag cgttaatcca
     8701 ttttgttaag aaagaggcta ttgattggag tatcgttggt cctgaagttc ctttgttaaa
     8761 tgggatcgtc gatgatttta tggcggaagg attgaaaatc ttcggtccga caaaagcagc
     8821 agctctgatc gaaggttcga aaagttttgc taaacaatta atggatactt atcaaatccc
     8881 gacagctaaa tccagaagtt ttgaatgtta cgaaaaagca gcagcgtata tcaaagaaaa
     8941 tggtgcgcca atcgtagtga aagcggacgg cttagcagct gggaaaggtg tagtcgtggc
     9001 taccacagtg aaggaagcgc tggatgcggc agaacaaatg ttgaaccagc atcgatttgg
     9061 ggatagtagc aaaaaaatcg tcgtagaaga gtttttggca ggagaagaat tctccctttt
     9121 ggcgtttgtc cgtaatacca aggtgtatcc gatggtcatt tcgcaagatc ataagcgggc
     9181 atttgataac gataaagggc caaatacagg cggaatgggt gcttattcac cagtaccgca
     9241 aatttcaaaa aagatgattg aaaaagctgt ggaagaagtt ttaaaaccag cggctttggg
     9301 aatgaaagaa ctaggacgct ctttcacggg tgttttatat gcgggtttga ttgctacgaa
     9361 ggaaggacca aaagtgatcg aattcaatgc gcgtttcggt gatcctgaaa cgcaagtcgt
     9421 tctttctcgg ttgaagagtg actttgcgca agtaatcgat gatttattag agaatcgtac
     9481 agtagagtta aagtggcagc aagaaggata caatgtaggc gttgtggttg cagctgccgg
     9541 ttaccccgaa gaatatgaga caggaatggt tctaccagat tttctggaag aagagctttc
     9601 tgtttactat gctggagttt cctcaaataa cggcaaacta gttggagcag gaggacgtct
     9661 ttatttagta gaagcatacg gtgagacgat caaagaagca cagaaaaaaa tttacaccgc
     9721 gttaatggat aaaacgacag atggaacatt ttatcgtaca gatattggag aaaaagctgc
     9781 caatagcgaa ctgatcgtga agtaaatcag ctgcttccat ttataaaaaa tacaacaaac
     9841 ataatcggaa gcgccgattt tagtgaagcc ttttcataaa gagtgtggta cacttttgtt
     9901 gtgtatataa ataaggagga gttcagatga aaaaaattca attaggtact tctgatcaga
     9961 aagcagctgc agtgatctta ggctgtatgc gtatcaacaa tgcggaagat ccagtaaaag
    10021 tgattgagac agcttatgaa aacggcattg atttttttga ccatgcagat atttatggaa
    10081 acggtgaatg tgaagaaatc tttgcagatg ctctagcaaa aacatcaatc aaacgagaag
    10141 atctttttat tcaaacaaaa tgtggaatcg ttccaggtgt gatgtttgat ttttctaaaa
    10201 aacatatcat tgaatctgta gaaaaaagtt tgacccgtct gaaaatggat tatgtggatg
    10261 ctttacttct tcatcgtcca gatacattgg tagaaccaga ggaagtagct gaagcgtttg
    10321 atgaattgga acggtcagga aaagttcgtt attttggcgt cagcaatcaa aaaccaatgc
    10381 aaatcgaatt gctgaaaaaa tatgttcgtc aaccattagt agctaatcag ctgcaatttg
    10441 gcttgaaaca tactggaatg atcgatcaag ggattcatgt caatatgaca gatgaagcaa
    10501 gtatcgatca tgacggtagt gtacttgatt attcccgatt gcatgatatg acgatccaag
    10561 catggtcacc ataccagtat ggattttttg aaggtgtatt tatcgggaac gaaaaattcc
    10621 ctgaattaaa ccaaacatta gaagaactag cagctaaata tcaaacgaca ccaactggct
    10681 tggcttctgc ttggatttta cgccatccag ccaatatgca agtgatcgcc ggtacgatga
    10741 acgtagggcg aatcaaggaa atcgctcaag cttcagaaat taatatgtct cgggaagatt
    10801 ggtacgaatt gtaccgtgct gctggaaaca ttttacctta aagattgtca taagcaggtc
    10861 aaaagggcta taaattaaaa agacagaata acttgaaagc tgttttcagc ttcgagatat
    10921 tctgtctttt tttattgcaa atggaaagag tatcattgta cggatttcgt tt
//
LOCUS       DAHHZJ010000099        10911 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.100, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000099 DAHHZJ010000000
VERSION     DAHHZJ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.100"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            147..725
                     /locus_tag="NBL02_001931"
     CDS             147..725
                     /locus_tag="NBL02_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCD2572611.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            725..1654
                     /locus_tag="NBL02_001932"
     CDS             725..1654
                     /locus_tag="NBL02_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD2572612.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            1651..3387
                     /locus_tag="NBL02_001933"
     CDS             1651..3387
                     /locus_tag="NBL02_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCD2572613.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(3450..4286)
                     /gene="pheA"
                     /locus_tag="NBL02_001934"
     CDS             complement(3450..4286)
                     /gene="pheA"
                     /locus_tag="NBL02_001934"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HCD2572614.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(4283..4792)
                     /locus_tag="NBL02_001935"
     CDS             complement(4283..4792)
                     /locus_tag="NBL02_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HCD2572615.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(4785..6080)
                     /gene="aroA"
                     /locus_tag="NBL02_001936"
     CDS             complement(4785..6080)
                     /gene="aroA"
                     /locus_tag="NBL02_001936"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCD2572616.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(6093..7184)
                     /locus_tag="NBL02_001937"
     CDS             complement(6093..7184)
                     /locus_tag="NBL02_001937"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HCD2572617.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(7201..8367)
                     /gene="aroC"
                     /locus_tag="NBL02_001938"
     CDS             complement(7201..8367)
                     /gene="aroC"
                     /locus_tag="NBL02_001938"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCD2572618.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(8369..9433)
                     /gene="aroB"
                     /locus_tag="NBL02_001939"
     CDS             complement(8369..9433)
                     /gene="aroB"
                     /locus_tag="NBL02_001939"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCD2572619.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(9466..10488)
                     /gene="aroF"
                     /locus_tag="NBL02_001940"
     CDS             complement(9466..10488)
                     /gene="aroF"
                     /locus_tag="NBL02_001940"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HCD2572620.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         3037 a         2404 c         2006 g         3464 t
ORIGIN      
        1 aaatcaacta tgacaaaagg aataatctta gcatctagac caaagatcga aatggtgagg
       61 tttctttaaa ataaaaatat ccttgcattt tctcctagaa ataagtattt ttaaattaca
      121 agactattct taaggcgggt aaaaaaatgc caatacgcgt aattcttatc gatgatgaac
      181 agacgatttt agatggaatg aaacagttga tcaattggtc tgagcacggg tttcaattaa
      241 tagggacttt ttccagtccg ctgaaagcgc ttgatttttc tttggtacat gcaatcgata
      301 tcgtggtcac agatgtaatg atgcctgaat taaatggaat cgaattatcg cggctattaa
      361 aacagcaaca acctgaaaca cagatcctta tcctctctag ctatgatgaa tttgatatgg
      421 ttaaagattc ttttaaagaa ggcgtcgctg attatctttt aaagcctcga ttgagtcctg
      481 atttttttct aaattcctta actgccgttt cacaaaaaat caaaaaaaag cccaacaaaa
      541 gcccaatcac caatcgttat gagcagatct gcgagaatct ttcacagcag attgctggac
      601 aaactcgtcc attgcttgac cctcttcttt ttgaacacac cttgtttttc tgttatatac
      661 cgaaaaacgg ccattcagtt caaaacaagg ctctaaacaa aaacagtcga tcacccattc
      721 attgatggat agcgacgatt cgtttactat ttatcctgtt tctacctttt cggaagaatc
      781 cggctatgta atcaatactg attccaaaga tacacttagg aagtttgttc aaaagtttcg
      841 tcaagaagtt tttccaggtg atttttttgt tgtttctgat tcttttgcaa tccaagatat
      901 caatgaaata ttccaagaga tcagaaattg caccaaagga caacgtttct atcataaggg
      961 aaagaacctt gcttatcagc atgaacttct tccgttatta accaaccacg agcagcaagc
     1021 aagtagttat ttaacaaaaa tcgttcataa agactacgct ttagctgtca aagaacttga
     1081 gaatttcttc acgacgatat tggaacagcc tattgatcca gcttatttga aacatgaggc
     1141 aatcaatcgt ttctatgcat tactcaacgc tttggctgac gagcatgttg ggaatgaaga
     1201 actttctttg ctcaagatcg cccttccttc tttattagcc gatgctgagt atttcgaaga
     1261 tttttctatt cttgtcaaag aaacacttga gaaactcacc cacttttctg caaatgcaaa
     1321 aaaagacaat ttcgatatct tgactttgat ctacgagttc gtccaggaaa attatgagaa
     1381 agaaatcaat ttacaacttt tatccgaaaa atatcatttt tcatatagct atctctcagc
     1441 catctttact gaaaaatttg gtatcaactt ttcaaaatat ctaaaaaaag tccgtatcag
     1501 taaagccaag ctctttttga cggaaacacg gcttactttg acggaaatct gtgagaaagt
     1561 cggctatacc gaactgggtt atttctccag agtttttaaa gaagagacag gaatgacacc
     1621 ttctcaatac cgtaaaggaa cttttgtaaa atgagaaaaa aaatcaaaga gcatgaactc
     1681 tttgccaaac ttctgttcat tttgttcatc ggtctttttc tgcaagccat cgtcatttct
     1741 ttgtttattt atcatcgatc aagggatgcc tatatccagc tgttcaatca atcgaatgat
     1801 gtggtactaa aaaagattca atctgaattt gaaagtttga atgatacaat cgagaataca
     1861 ttagccgctt ttgattctaa tccagctgtc aaaagctatt tttccaatga tccagcccaa
     1921 catatggaac agcttcagca actaagaaca atccaaaaaa tgaatgactc tctttcaaaa
     1981 atccatccaa tgatcgatta cgatgtattg atctttggag aaaatggaag aacttttgtc
     2041 ggaaacgaca tgctcactgc cgtatctgcc gattcttttt ttcaatctgc gattgctcag
     2101 agagtgaatg aacgtgctgc tgatacacaa atgctttttg cacaccacgg attgactctt
     2161 cgcgataaga aagcacccag cgtcttcttt gtcaaaaaac tgaaaaatac attgaatcac
     2221 gtttacggat atgctgtttt atcgatctca tccaagcaac tagcgaatct ttttcaatct
     2281 gtagtaaatc ctgagatatc gaagatctcc ttcatgaacg aagaacagac gatcatcgca
     2341 tcaaatgaag aaaatacaat tgggagccga tcaatgattt tcaaaaaact aacagtaggt
     2401 gaaacaacga tttcaaatgg acagcagttg acccgtcttc ctctgtaccg ccaggattgt
     2461 acattactca gtcaagtcga tcttcattct ttatctaacc aaatgggcgt gatttttccg
     2521 attattttgt ataatatcgt gacacttgct tttgctggct cattggtatt tatgtatttg
     2581 gatcgacaca caaaatcgat ctaccgcttg attgattcgc taaaacggct agaggaagat
     2641 ggcttaaaag ctactgttcc tgtacaagga agccatgaag tccaattgct agggaatacg
     2701 atcaatcaat tgctaagtaa tgctcagcat cattatgaac agacgatcca agacgaaaag
     2761 aagaaacgtt cgcttgaaat ccaaactatg caagcacaga tacagcccca ttttatttat
     2821 aatacgctga ctagcttgaa atttttgatt tggcagaaaa aaacagatga agcagttcaa
     2881 ggaatcgaca gtttcattac tttactaaga agtacgattg ggaaaaaaga agaagtgatt
     2941 ccagtcaagg aagaaatcaa aagcgtacaa agttacatcg atcttttgag tatgcgatat
     3001 ggcgaaagaa tctcggctaa aataatgatt cctgatgaac tgcagtcatt atctatgccg
     3061 aatatgatcc ttcaaccaat tatcgaaaac gcctatcttc atgcttttca caataaaacg
     3121 caaggattca tcacggtgta cggcaaaaca aaaaatcagc ttcttttatt cgaagtgatt
     3181 gataacggcg atggcttcga tactgaaaat accaaacaaa aaaatgatca tttttcagga
     3241 atcggcatca gccatgtaga tgagcggata cgcctaatgt atggcgatgc atatggctta
     3301 acgatccaat cagtgattgg gacaggaacg actgtaacga tttgtctacc gcttcttcct
     3361 aaaaatcaat cagaatatca aaaataaaga atagccaagc gctgattttt ttattttaag
     3421 ttaaataaat caatcagtat tcttgacttt catctacgcg tctcttctac tgtcagcact
     3481 ggataacagc ccaatatttg actttctcct ccgagcatct tgatttcttg gagcgcattc
     3541 tctactaatg tcattggacg gtccaacaac aaatcgatca cgaaaaaata ttctcccaag
     3601 ctagttttca atggtcggga ttcaatctta cttaagttga tttcccgcca gccaaaagct
     3661 gccagcattt tatgaagcat ccctggccga ttagcaggta gtgtcaagat gacactcatt
     3721 tttactggag ctgactgctg gctggtcatc tttcggtcac cgacgatcca aaaacgtgtc
     3781 tggttcaact cattatcttg gatattctca gctaagatct ccaatccaac atgctgtgct
     3841 gtttctaatg aagcaatcgc tgctgcgtct tctttcggat gttctgctac atacattgct
     3901 gcagcagttg ttgattctgt tgctactaaa ggaacattag gataatgcgt ttctaaaaac
     3961 tgttgtgatt gtgctagtgc ttgtggatgt gaaaggattt ttgtaatctt tgttgctgga
     4021 ttgcctaaaa gctgctgctt gataggaaga acgatttcac ttttaacttc gacttctgga
     4081 tgtttggaaa gaagatcgat tgtgtgatga acagctcctt ccaatgaatt ctctacagga
     4141 acgacagcca gatcgacttg tttgcgaaac aaagcgcgca aacaaacagg gatggaagca
     4201 tacgcagtca attgttctgt aggaaatagc tgacaagccg cctgataagt gaatgaactt
     4261 tctggcccta gatagctgac gttcatatgc ttttgatcct ttctataatg gtatcgatga
     4321 tcttttctgg tgtttgattg gtcgtgttga ttcgatgtgt cgcactttct tcataccaag
     4381 aaagtcgttg tgccaataat gcttttattt cttctcttgt ttttccaaga gctaaaggtc
     4441 gaatagacgt ttcatcttgt tggatacgat caactagaca gtccgcttct gcctctaagt
     4501 aaagaacaat cggctgtgtt tttaaaaaac gccgattttc tgcactttgg acaatccctc
     4561 caccagtcgc gataattttg tcttcatttg acagctttct taaaatatct gactccactt
     4621 ttcgaaagct tttttcaccg taatagtgaa aatattcggc aatcgacaat tggatatgtt
     4681 cctcaatctt tgtatctaaa tctacatact ctttttgtaa acattgagct aaccctttac
     4741 cgattgttgt ttttccagcg cccatgaaac caatcagcaa aatgctagcc atgagcttct
     4801 cctcctgaca caagttctgc taaatcgtca aaaaatgccg gataagagat tgaaacagct
     4861 tcagctcctt ctagttcgca aggtccatca gctatcaaag cagctacttg cagcatcata
     4921 ccgattcgat gatcgcctct gctggacact tttgcgccat gcagaggtgt cggcccatgg
     4981 atgatcagac cgtctgctgt cgcttttatc tttgctccca ttttctgtaa ttcttctgcc
     5041 accgcatcaa ttctgttggt ttcttttact tttaattctt ctgcgtcttt gatgatcgtg
     5101 attccttctg cttgagtagc cactaaagct agaatgggca attcatcaat cagacgcgga
     5161 atgatttctc caccaatcgt tgctttcttc aaatgactcg tcttgacact taaatcagcc
     5221 gattggttct gttcattgtg attcgtctgg gtaatcgctg cgcccatctc ctccaacaca
     5281 tctaagatac cagttcttgt tggattgacc ccgacatttt ttaacaacag ttggctttcc
     5341 ggaacaagaa gtcctgctgc tagaaagaaa gcagctgatg aaatatcacc tggaacagtt
     5401 atctgttgac cagttaattt ttgcggtccg gtcactatga tcgttttatc ttccactgtg
     5461 atccttccgc caaattgacg aatcatttct tccgtatggt tacgggaacg ttctttttca
     5521 acgattacgg atgtgccttc tgcttgtaat gccgcaaaca gcaaagcaga tttgacttgc
     5581 gcactggcta caggcatatt gtatcggata ggtgataaag agcgggttcc tgtcacagtg
     5641 attggtagat actggtcatt ttcttctcct tgcaagtcag cgcccatctc gcgtaaaggt
     5701 tccatcactc gccccattgg acgtttagac aacgaagcat ccccgaacag agtagtcgta
     5761 aaaggctgtc cagccaaaat acccatcaat aaacgtatgg tcgtaccgga gtttcccatg
     5821 tcaattgatt cggttggttg gacaaatgag tggctaccgc gaccatgaat cttgatcacc
     5881 tcttcctctt cttcgatctc gacacctaat tgttggaagg catgtaaagt actcagacaa
     5941 tcttctgcgc gcaaaaaatt ttgtatagtc gtcgttcctt cagcaattgc tccaaacatg
     6001 atgctgcgat gggaaattga tttatctgct ggtatcctaa cagtccctct taatccatgt
     6061 atttgttgta ataattgcat ttatccagcc tcctatttga tccagcaatg ataactggta
     6121 ttagacataa tgattctttt tgcttgtaac aaatcctgtt ggtttttaaa actgatttgc
     6181 agtattccta aaatatcttc cctggtctct tggatcttca aattaatcaa agaaactccc
     6241 gcttttccga tcagtccagt tacttctgca ataacgccag gttcatctgg cacatcgata
     6301 aataaatcaa aaaaggcagg aatcgcgcct tgcttatgga caggcaactg gtctcgactt
     6361 tcttttgccc gattgaaaaa agaaaagatt tccgattggt tctcagtcat gatccattct
     6421 gctatttgtg tcatttcttg ttgccaatct gccaatcgtt ccaataatat ctgcttattg
     6481 ctcaacaaaa tatctgtcca catgacagga tctgaggaag cgatcctagt gatatctcga
     6541 aagcctcctg ctgccagttg tttggctcgc ggatactctt gattgaattg ttctgcctga
     6601 ttgactaagc cagctgccag aatatgtgga aaatgactta gcattcctgt tatacggtca
     6661 tgttctcttg ctgtaagagt tacatatttt gctttggttc cgcaaaatag ttcttgcagt
     6721 ttttcaacat cttttttcgt cttttccaaa ggtgtaaaga tgtaataggc attttcaaat
     6781 agtttagcat ctgctgcctg tatacctgat ttatgagaac cagccattgg atgtccaccg
     6841 acaaaccgaa gatttttctg ctcagcggat gtcacgactt gctgcttcgt actgctgaca
     6901 tccgtaatga tgacgtgatc agacaagttg attctttcaa ggacatcaag ataagctaag
     6961 gttgtacgga cgggaacggc taataagata acgtctgctt gttctgcacc tgtttcaaaa
     7021 tcacttgcaa cgtaatcaac gatctgctgt tctactgctt gctcacgggt ttttgactga
     7081 ctatcccaac caactactat cagttctgga ttggtctgac gaatacaaag tgccatagaa
     7141 gcaccaatca gtccaagccc cacgataaaa acatgttgtg ccatcttatt tgcccccttt
     7201 ttagtaatgt ttcaagtagt cacggtactc cttcactgca gctttcaact caacaagcga
     7261 atcacttggg aattttgaca atagctctgt cgctaccaca gtggctacaa cattttccgc
     7321 aacgacgcta gctgcaggaa ctgctgtact atccgaacgt tctacacttg ctttgtaagg
     7381 ttcttttgta tcgatatcaa cactttgaag tggcttgtac aatgtaggaa tgggtttcat
     7441 gaccccgcga atgacaagtg gttcgccatt ggtcattccg ccttcgaagc cgcctagatt
     7501 attggtacgt cggaaatagc ctctttcttc ttcccatagg atctcatcca tcacttctga
     7561 gcctggtaat gaaccagaag tgaatccgac cccaaattct acgcctttaa aggcattgat
     7621 actaaccaca gcttgtgcga ttttggcatc tagcttcgtg tcccattgta cgtagcttcc
     7681 taatccagct ggaacgcctc caacgagcac ttctacaatt cctccgatcg tatctccatt
     7741 tttctttgtc tgatcgatca aatcttttat tctttgctca actgctggat caacaactcg
     7801 tacttctgac gcttcagaaa tttctttgat ttcttgaatc gagatatttc caggtatttc
     7861 tgcttggatt ccaccaagac aaatcacgtg tcctgctacg atgatgtcaa gttcttccag
     7921 aattttcttg gcaattgcgc cgattgctac acgcatggtc gtctctcttg cagaggaccg
     7981 ttcaagcaca ttccgaagat ctcgatgatg gtatttcata ccgcctacca aatccgcgtg
     8041 tcctggacgc ggacgtgcaa ctcgccgcag tgattggtct ttttcaggca cttctgctac
     8101 agacatgaca gttgtccaat ttttccaatc tttattttct accactaaag tgacaggtga
     8161 accaattgtt ttgccgtgac ggatgccaga cgtgatccga acacgatctt tttcgatctt
     8221 catccttcct cctctgccgt aaccagcttg tctgcgagcg agttcgtgat tgatttcttc
     8281 ttctgacaga ggtaatcctg ctggtagtcc ttcaataatg gctgttaatt ctggtccatg
     8341 ggattctccc gcagtcatat aacgcatctt acagtcctcc taagtaatct ttgaatgtct
     8401 ctgtgggaac aggaacgatt tttgcttgcc caatcttttc tagcaagatg atattcaatt
     8461 ggcttcctct tgctttctta tcatgggtta tcgcgtggaa taattcatct ggatgactta
     8521 gatcgtacgt aatcggcaga tggaattttt gaagtatcct ttctaaagca gctgtcgatc
     8581 cagttggaga caagccgatt ttttctgcat gagaagtgat ggctaccatt ccaatagcta
     8641 ctccttctcc atgactgact accccatatc ctgctgtatt ttcaattgca tggccaattg
     8701 tatgaccaaa atttaaaata agtcgatttc cttggtcaaa gacatcttct tccactactt
     8761 ttcgtttgat ttccaacgta gcagtaatga ttggaacagc atgttccaga agatcttttt
     8821 cgtctttcag ctcgttcagc tgtttccaaa gcgttgcatc tgctatagct gctgatttga
     8881 tgatttcagc gattccttct cgtaaacgac gtaccggcaa agtttttaga acctcaggat
     8941 caatcaatac accagctggc tggctaaatg ttccgacaag gtttttcgct ttgtttgtat
     9001 tgactgctgt cttcccgcca atactagaat ctacttgtgc aagtagcgta gtcggaattt
     9061 gaacgaaatg gatcccacgc atgaacgtcg aggccacgaa tgctgctaga tcacccacca
     9121 ttcctccacc aagtgcaagt atcccatcgc tgcgggtaaa atcattatct gctaaaaaat
     9181 cgtataactc agctgcttgt tctaaagatt tactggtctc acccgccggt acgattgaaa
     9241 ggactggttc aaaattagct tgtctcaatt ggaacatgat tttttctgca tatagtggag
     9301 caacgtttgt atcagtgatc acagctactt tttgaggtct ccaaatcgtt tgaagccatg
     9361 atccagcctg ttctagtgcg cctttttcaa tatgaatatc gtatttttgg ttatcaacat
     9421 gtacagtaag cattcgtttc acttctttct tttaaaaagc tggaattaat cgagtgtctg
     9481 aatcttttcc attgcttctt tcatgatcgc aacttctttc atcatgcgtg agtaagtctt
     9541 ttcattcaac gattggggac cgtctgacca agcatttgca ggatcaggat ggatctctac
     9601 gatcatgcca tctgctccgc ttgccacacc cgcacgagcc attggtggta caagttccca
     9661 aattcctgta ccatgacttg gatcaacaat cacagggaaa tgagttaatt ttttcatcaa
     9721 tgggacagca cttaaatcaa acgtattacg tgtcgctgtt tcgtaagtcc gaatcccacg
     9781 ttcgataaaa atgacaggag atttgtcttc gactgcaatg tattctgcag cattaagcca
     9841 ttcattgatc gtaccggaaa ttccacgttt caatccaaca ggtttacctg ttttaccgac
     9901 agcagatagc agtttaaaat tttgcatatt acgtgcgcca atctgtagga tatcgctgta
     9961 ctcagccacc atatcgatat gcccttcatc catcacttca gtaatcactt tcattccaaa
    10021 ttcatcagcc gcttgacgga tgtatttcaa tccttcttct tctaaacctt ggaaagcata
    10081 tggagaagta cgtggtttga aggcaccacc tcgcagaatt tttgcgcccc cggctttggc
    10141 cattcgcgca cattctctga tttggtctaa tccttcgata gaacaaggac cagccatcgt
    10201 cacgaaactg ccgtcaccaa tttttactcc gtctacatcc acgactgtat tgccaggatg
    10261 gaattctcga gaagtcaatt tgtatgtgtt taaaatacgc acagcatttt ctactccttc
    10321 atagcgtgta aatgctcctt cttgtaaact tcgcgtatca ccttttagtc caatcactac
    10381 tcgttctgtt ccttgatcga ttgagacttt gaatccttct ttttcgatat gatcgacgac
    10441 tttttttatt tgttcttccg ttgcttcttt tttcataatt actatcattg tttttcccgc
    10501 tttcttttgt ttacttatct atcttggctg attttctcta ctttttcaag gtggttttac
    10561 attaattcct actaaatgac cgtgtcctgt tgttctcatt ttcgctcctt ctttcctact
    10621 tatgacccag ataatcaaca aaaaaagatg tccctgtagg aaattgcctc tacagtgaac
    10681 atcttcaacg agccttaaaa agcgctgcaa aaatataaaa tagccactat agatgaattt
    10741 ctctacgtgg ctgtaagtcg tgtttaaaaa tctttagcca tcatagatac aaacctggtt
    10801 tgtttgtacc cacgaagtca tgaatacaaa cctatctaaa atagctaaag taatacgtat
    10861 tcagttttgt aagttttcta gatttcataa aaaatcgctc caaactgatg t
//
LOCUS       DAHHZJ010000100        10841 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.101, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000100 DAHHZJ010000000
VERSION     DAHHZJ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.101"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(345..569)
                     /locus_tag="NBL02_001941"
     CDS             complement(345..569)
                     /locus_tag="NBL02_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572621.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            760..3537
                     /locus_tag="NBL02_001942"
     CDS             760..3537
                     /locus_tag="NBL02_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCD2572622.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3618..4037
                     /locus_tag="NBL02_001943"
     CDS             3618..4037
                     /locus_tag="NBL02_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HCD2572623.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4049..4519
                     /locus_tag="NBL02_001944"
     CDS             4049..4519
                     /locus_tag="NBL02_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCD2572624.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4536..5315
                     /locus_tag="NBL02_001945"
     CDS             4536..5315
                     /locus_tag="NBL02_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCD2572625.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5302..6123
                     /locus_tag="NBL02_001946"
     CDS             5302..6123
                     /locus_tag="NBL02_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCD2572626.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6140..7189
                     /locus_tag="NBL02_001947"
     CDS             6140..7189
                     /locus_tag="NBL02_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCD2572627.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7202..8206
                     /locus_tag="NBL02_001948"
     CDS             7202..8206
                     /locus_tag="NBL02_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCD2572628.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8839..9858
                     /locus_tag="NBL02_001949"
     CDS             8839..9858
                     /locus_tag="NBL02_001949"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCD2572629.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(10024..>10841)
                     /locus_tag="NBL02_001950"
     CDS             complement(10024..>10841)
                     /locus_tag="NBL02_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCD2572630.1"
                     /translation="TRYQLIKQLIRTKQHFLENLTYKCNTLAREMRDESTSLFSATLI
                     SLMTEDFTLDELAELPLEAFCDLLQEKGKGRFKQPEKIAKAIQRAITMSYRLGALAQE
                     SINVVLSVLVREIRALEKNIKELDKAIEQVIVVIPEYQCLTSIPGVGKVYAAGLIAEI
                     GQIERFEDQTKLAKYAGLSWKVNQSGNYQSQNTPLTKQGNRYFRYYLVEAANSVKNYL
                     PEYKAFYQSKYKEVPKHQHKRALVLTARKFTRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT         3577 a         1808 c         2254 g         3202 t
ORIGIN      
        1 aataatagtg gagaaaacta tggtttggcg cagattctat caggtttgat ggaacgaaga
       61 caacctcccg acatcaagtc aaaagctact tgagattttg atctgaaaat aaaaaaagaa
      121 aacaaggtag gaacatcaat gtttctacct tgttttcgta atcaatggga aaaaactaag
      181 agtggtgtac gtaatcaata aaaaggatgg tgacaacagt acctctttac tctctgaaag
      241 catcataact cgtttttcct cattatgcca ataaattgtt gttataccac atataaggat
      301 ttgtaatagc taaaaacaga caggaaagtt gaaatctgtg aatcttattt tgtatggaag
      361 gatttataga acgcaaaaat caagataaag atgaataaaa atcctgtttc ggctatatat
      421 tgcgtatcta cgccaataaa acgagtttgt tctgtcatag ccatataaag caaaggcaaa
      481 aatacaacta cagcgagata agatccgatg atttctcgaa aagttttcca ggaagtatgt
      541 ttcctgtttt tatatttatc cgatttcatt gaatagtcat agcctcctta ttctttcata
      601 tgttgaaatt ccagtataaa aactggaagg aactgtacgt tttttagaat aaaaattata
      661 agggataagt aggctgctgt ctataataag atatattttt caattcttta ttaaagattt
      721 tattgacaaa atgaatccgt tttctatgat ggaggagggg tgaataagtt gttgaagaag
      781 gaaattttat gttacttaga gaaccagaca gcctttttcg atttagaaca tatgaacgag
      841 gtctttacag cacaaaggtt agctgatcat tttggggtaa aaagaaacac agtcagtcat
      901 tatttgaacc agttaaatga tgagcaacta ctagtcaaaa tcaattctcg accagtgatt
      961 tatttccaca aagaagcttt tgaaaaacaa ttttttaagt tacgcacgaa tttttatttt
     1021 tctatcgatg atctcaaaga ggaacagcct tttttcgcgc aaccaaaaga cttattctct
     1081 tttatgatcg gatacgacgc cagtttgaaa gaaagtatcg aacaaatcaa aacagctctt
     1141 tactatccag atggcggatt accacttttg attacaggcg aaagtggcac aggtaaaagt
     1201 tatttagtta atttggtcta tcagtattgt ctgcttcatg atctgctaga agattctgca
     1261 ccattcatca cttttaattg tgcacaatat gcagataatc ctgaactttt gactagcaat
     1321 ttgtttggac atgttaaggg agcgtacact ggtgcagaag aaaataaaaa aggggctttt
     1381 gaagcagcag atggtgggat cttgttttta gacgaggtac atcgcttaac accagaaggt
     1441 caggaaaaac tgttcaccta tcttgatcaa ggtgtgattt atcgtatggg agaacctaat
     1501 ttgtcacgcc ggatcaaaac acgtctcttt tttgctacga ctgaagagat tactagtcat
     1561 ttcttaacaa ctttcattcg aagaatacct atccgaatcg aactacctgc acttaatcag
     1621 cgtagtcgta atgaacggct agaactgatt tactcttttt ttctcgaaga acaaagaaaa
     1681 atggggagcc ctttgtcagt cagtggacca gctttgtcac ttttaacaag tcagaaatta
     1741 tccggaaaca taggagagct gaaaaatatc gtcaaagttt cagcagcaaa agcatatgct
     1801 gaacagcgtg aacagcctga aatccatgtc acgatccatc atttgcaaaa agaactactt
     1861 gcgcagccta ttactaaaaa tacagcacag caaggaatct atattacgca agaccagacc
     1921 ttagagcaat taatggagaa gcaacagccc gaacagcagc gaatcgttaa aagttttgag
     1981 caaatcctaa ttgatttcaa gaaaaataac tgtctgctat attcttgcga aggaaaatta
     2041 aagcaagaag tgattcaatt atttgatttt ttgctgtttg agaccagtag acaagaaaaa
     2101 cacgagctgc tggtttattg gacacaatct atccgagaga cctttcgaca aatggaaact
     2161 gcctatcaaa tcaaattcga tggaaacagt gtctatgcct tgagttacta tctctatcag
     2221 cgaagcacta tcaaatggtt accggaagaa catgatatta tccaactaat caaagaatta
     2281 gaacaacaag tttctcagtc ttatccatct agttatcact atgtccaacg aattctggaa
     2341 ttaagtaaga tcaaattaga tattgaagac acaagtatgg atcggatttt actgacgatc
     2401 tatctaaaaa aagcgaaatg gtcaaaagaa aaacgactgc caaaagcgat gattgctgct
     2461 cacggttatg caactgctag cagtatggca aatgtagtaa atcgtttatt acaagaggat
     2521 ctatttgaat catttgatat gccactagat gtcacaccac agcagattgc ttcagaaatc
     2581 cttgattatt gcgaaaacag cgatgtttca aatggattga tcatattagt agacatggga
     2641 tctctgaaag aaatccatca gttttttaaa aaacagttat ccgtaccgct tttgattttg
     2701 aataatgtga cgacaccttt agcaatcaca gtaggggaat gcctgcaaaa aaatgtatca
     2761 ttagaagaga tagcagaaga gactgttcga cagatccagc cagaatggcg actgctctat
     2821 ccagaggaaa acaaacccaa agcactcatt actacttgct tcactggaat cggtacggcg
     2881 gtccacttaa gtgaactgct agaaaaaagc ttacctacgg cttgtcagtt gaaaatcatc
     2941 ccatatgaat atcagcaatt aaaagataag aaaaacagtg acccattatt ttccatatac
     3001 gaagtcgtag gaatgattgg caccacagat cctgatatca ccaatatcga ctatctttca
     3061 ttagaagacc tcatctcggg ggaaaagatg tctgtactag ctgaatggtt ggacactact
     3121 atgaatgctt cagagaagga aatattcaat cagcgcatca ttcgtaattt ttcattggaa
     3181 aaagtactgg attccgtgac tattctagat acagagaagg tgatgggaga aatcgatcat
     3241 tttatgagag aattagaggt gcagttgaat cgaagcatca gcaatcccaa aaaattagcg
     3301 ttgtatgtcc atgtgagctg tctcattgaa cgattgatcc gacaaatgcc aatcgaaacc
     3361 tatcaaggat tagagaaatg gaagcagtgt caaaaagaag ggttatctgc tattaaatct
     3421 gcgtttagtg tcattgaaga gcattatagt gtcatgatcc ccaattctga gattgcctat
     3481 atctatgata ttttgagtga agatacagag tttctctcga tagaagaaga attttgaaca
     3541 taagcaagtt gttgccacta attttggcat gcaacttgct tttttagtat gtgtaagtga
     3601 aaagaggaga tgaaaaagtg gatagagaaa ttatcttagc ttcccatgga aaatttgctt
     3661 cagggatatt agattcatta gagctgatct atgggaaaaa tcattctatt actgtattcg
     3721 attgctatac tgatgagaat tttgatttag cggaaacagt aaggcagctt tttatgaggt
     3781 acaaagataa agaaatcatt gttctgacgg atctttttgg cggaagtgtc aacaacgaat
     3841 tcttacaata tatcaatcgg gatcatgtct atctcgttgc cggattgaat ttgccactat
     3901 tgattgagct agtatctcgg ttggacgctg aagtagagac aactgttttg atccagcagg
     3961 tgctaactga ttcaaaaaaa atgatccagt tttgtaacga gcggctcaat cagaaaatag
     4021 aagaagacga attttaggag gaagaaaaat gattgtattg acaagagtcg accatcggtt
     4081 attacacgga caagtagctt tttcatggac acaaacaata ggcgcagact gtattttgat
     4141 tgctaatgat gatgtaccta cgaatgagat acgaaagaca acgatcaaat tagccaagcc
     4201 acaaggagtc aaactagtga tcaaatcaat tgatgactcg attgccgcgt tgaaaagcgg
     4261 ggtgacagat aaatataagt tattcatcgt agtggaatcg atcgaggatg catataagct
     4321 ggcagaagcg tatccagaaa tcaaaaccat caacttagga gggatcaagg caaaacctgg
     4381 tactcgcagc atcggaaaag cggtaaatat tttaccagaa gaggaggaac gcttgaaacg
     4441 attaattgac aaacaaatag aagtagaaat ccgtcaagta ccagcagata aaaaagtgga
     4501 tgtaagagaa gttttgtaag aaaggaagaa cctcaatgat tgtacaagca gtcttattag
     4561 gaattgttgc atttgtcgca caatcagaat atgcactagg gacatcgttg ctttctcgtc
     4621 cgattgtgac ggggctgttt acaggaatcg tgttaggtga tgtaaaaact gggatcatta
     4681 tgggcgcaac tttagagtta gcatttatcg gatcgttctc agtaggtgct tcgattccac
     4741 ctgatgttgt gaccggtggg attttaggga cggcttttgc tattacagct ggagccggta
     4801 cagaaaccgc gcttttacta ggtttgccga ttgctacgtt gacattgata ttgaaaaata
     4861 tttacttagg gcttttgatt ccaattatga accataaagc agacaattat gcagaagaag
     4921 gaaattataa aggagtcgag catatgcact tgcttgccgg tatcggtctt tccttgatgc
     4981 ttggtgttgt ggtaatggtt tcttatatgg tgggaagcaa tacgatcgga aatctattaa
     5041 atatgattcc tgacttcgtt caaaaggggt tgtcagtagc tacagggttg attccagctc
     5101 ttggttttgc gatgcttgct cgtttgctga tcaacaaaca agttgcacca tattttttct
     5161 taggttttgc tatagcttct tacttagaga ttcctgtgac cggagtagct atttttggtg
     5221 cgattttagc agttgtggta gtcaatatta tgaatgtccg tcaactacca cttcaaacaa
     5281 ctgcagggga ggtagaagaa gatgaagaat tctgagatca aaaacagcaa aataacgaaa
     5341 aaagagctaa atcacgtatt ttggcgttct tttcaaatgg agttttcttg gaattatgaa
     5401 cgtcagatga atatggctta tgtttatgca atgattccga ttttgaaaaa gttatatcag
     5461 tcgaaagaag aaatggcagc agctttgaaa cggcatctag agttttttaa cacgacccca
     5521 catatcgtta cactgatttt aggaatcacg gctgcaatgg aggaagaaaa taaggatgat
     5581 cctgaatttg atgtgactgc gatcgatagt atcaaaacat ctttgatggg acctcttgca
     5641 gggttaggag attctttctt ttggggaact ttgcgattga ttgccacagg tgtgggtacc
     5701 tctttagcca tgcaaggaaa tattttaggc cctatcttgt tcattttgat tttcaatatt
     5761 ccgcacattc tgtttcgcta tctagcaaca ggttggggat atcgcttagg aacgggtttt
     5821 ttgaaaaaga tccaggcaaa cggtatgatg gaaagtttga cattaggcgc gtctattatt
     5881 ggactgatgg tcgttggagc aatgactgcc acgatgatca ccatcaatat tcctttaaaa
     5941 atcggttcag gagaaaatgc tgtgactgtt caaagtattt ttgatgatat cgttccaaat
     6001 atcttatgtc ttggagcatt tggcatagtg ttttatctat tgaagaaaga agtgaaacca
     6061 ttaacgatct tgatcggact agctttcttt ggaatttttg gttcgtggat agggattttt
     6121 taaaaaagga gcagagaaaa tgtttacgat gcaagattat atctatgaag aaaaggaagt
     6181 actaagtacg atcttgaaaa agaatgattt ttcgacaaga gaacatatga aaaaaacagt
     6241 gaatttactg atcttagcaa cgggttcatc ttataacgct tgtttagcag cgaagccagc
     6301 attggaatct tatggtgatc ttactgtcga tattcaagaa cctttttatt ttttcaatta
     6361 tggaaaactt tctccttcaa tcgatactgt gattgctgtg tcacaaagcg ggaaaagtgc
     6421 ttctactgtt gaagcagtaa aaatgataca aaaacaaggg ttacctgttg ttgctatcac
     6481 aaatgacgtg caaagtccac ttgctttaga agctgcacaa attattgatt taggtgccgg
     6541 ggtagaatca gtaggcttcg ttacaaaagg ctattctgcg actgttttac agttactact
     6601 cttagggata ggaattggaa taagtaaaaa taaaatcagc aaaaaaatag aacaagacta
     6661 catgcaacag ttgcgcaaaa taatcaacca tttaccagcg attattcaaa aaacggaaga
     6721 attttttgat ggatatcaaa gtttatttcg tttagcacag cgtttcgtca ccattggata
     6781 tggtccaaac tggggaacag ctaaagaagc agagacgaaa ttgactgaaa caatccgcgt
     6841 tccgtcacaa ggatttgagt tagaagctta tatgcacggc ccatacctgg aggcagatgc
     6901 ttcgcatttg ctattcttta ttgaagggga aagtgtaaat aaggaacgtt cgcaaaagtt
     6961 gcaaagatat atgagtcggt acgttggaga gactctcacc attaccacaa aaaaagcaag
     7021 aaacgagaaa acacttggat tagcaataga atgtgatgaa tatctgtctg tattagcgct
     7081 tgtcgttccg tttcaactat tcgcttataa aaccgcagta gctaaaggta ttgacttgaa
     7141 caaaaagatt tttgaagact ttgatacagt cttgaaaagt aaattataag aggtggaaat
     7201 catgctgaaa ttcaatgaag aagaacaaat caaagcaata aaaggcgcac tagctttacg
     7261 tccgcaagta gaagaaatca tcgataaact ttatgcggaa aaatttgacg ctgtctatta
     7321 tctaggaatc ggtggaacgt atgcttcctc gatgcaagct gtcacttata tgaatgggaa
     7381 aagcaacttg cctgtctttg tacagcatgc tgcagaatat tacactactg gaaacaaaag
     7441 attaacaaaa gattctatcg ttattttgtc ctctgttacc ggtacaactc aagaagtagt
     7501 gaaagcagta gaacaaatca agaaagtagg agcgacactg attggattta tcgataaagc
     7561 gaacagtaag ttgtctcagc tatgcgattt tgtggtcact tatccagctc ctggaacaga
     7621 acaaatcaaa ttttttatgg cagcagatcg attgatgtat ttgaatggag aattcgaagc
     7681 ttattcagaa tactatgaac agctcgaacg ttatttacca acagggttag tggaagcaga
     7741 gaaaaaagca gatgcatttg gcttatcatt tgcagaaaaa catcgacgtg atagtatgca
     7801 ttattttatt ggagcaggga atcaatgggg agctgtttat tcatatgcca tgtgttactg
     7861 ggaagaacaa agctggcttc catcgaaatc tatccatgct gccgagtttc ttcacgggac
     7921 gttagagatc gtagaagaaa caaccccagt cacattattt ttaggcgaag atgaacagag
     7981 agtgttatct gaacgtgtgg ctaaactttt gccaaaaatt tgcagcaatt atacattgat
     8041 agacacaaaa gactatccag tagaaggtat cagtgaaaaa tatcgcggac gtgttttgtc
     8101 tttcttattg atgcatgtag tgacacaacg catcgatgct catgtggagc agttgaactg
     8161 ccatccatta gagatacgcc gctactatcg tcagtttgat tattaggata gactggataa
     8221 aaagaatata aaaaataaag agagcagcga taactgctcc ctttattttt ttaaaatatt
     8281 ggcatcaatt acgttataaa acacattttt tgcattatct acagttcaac ttataatctg
     8341 tttaatggga catgtgggtg ccaaccattt tttgttatgc aatagtacta tttagttgtt
     8401 gcatggatcc gtagtaaaag tccaagtaca acgagttgcg ccagtttaca tagcgttttg
     8461 tatccagaat gttgactaaa acaaaagttc ctatgtcaac cgtcaacaga gacaatctgt
     8521 ccgttgcagg acctgacaga tttagaaatg tccaatctgt ctcttatgta ttttaaatca
     8581 atactcacta ggagaagttt cctaacctcc taatagtaat gctggtagta acattgtcaa
     8641 aactgcttcc tcttaggaac aagcaaagca taacgagtgt aaaaataaaa tgtagactat
     8701 tgtaacatat cattgattat ttcaagtaaa aacgataaaa gaagtaaaca aacacttaaa
     8761 aacaatggaa attagtccca actttaagaa atgtatacta taatggaaat tatcaattat
     8821 ttatttggag gaatatccat gaaacaaaaa atcgcagttc ttggtcctgg atcttggggc
     8881 actgctttag cacaaacatt ggcagaaaac gggcatgatg tccgtatttg gggaaatgta
     8941 cctgaacaga ttgatgaaat caataagtat catacaaatc agcatttttt acctgatttg
     9001 acgatcccag aatcgattat cggatataaa gaattggcag aagcagtgga taacgcagat
     9061 gctattttat ttgttgttcc aacaaaagcg attcgttcag tcgcacagga acttattggt
     9121 aaaatgaata ctaagccaat cattatacat gcaagtaaag gactagaaca agatacgcat
     9181 aaacgaatct cagaagtttt ggctgaagaa attccagaag aaaaaagaca ggcaattgtt
     9241 gtattatctg gtcctagcca tgctgaggaa gtggcagtac atgatatcac aacaattaca
     9301 gctgcaagta ttgatcaaaa agcagcagcc tatgtacaaa aactgttcat gaatgattat
     9361 ttcaggattt atacaaataa tgatgtgatt ggcgtagaaa ctggtgcagc actgaaaaat
     9421 atcattgcaa ttggtgccgg cgctatccat ggattaggat tcggagatga tgcaaaagca
     9481 gcaatcatga ctcgaggatt ggcagaaatc agccgtcttg gtgtcgcgat gggtgccaat
     9541 cctctgacat ttatcggttt gagtggtgtg ggggacttga tcgttacttg tacaagtgtt
     9601 cattctcgca attggcgtgc cggtaacttg ttaggacagg gacataagct ggaagaagtt
     9661 ctagaaaaca tgggaatggt cgttgagggt gtggcaacga caaaagcagc agtagagcta
     9721 gctcaacagc taggtgttga gatgccaatt actcaaacaa tctataatgt tttgtataat
     9781 ggagaagata tcaaaaaagc agcaaaagaa atcatgttac gtgacggaaa aatggagaat
     9841 gaattttctc ttcgttaaat agatgatcaa tagttaatcg tcgtttagtc taatggtgat
     9901 aagtcaagat gaaatttaag attttcttta ttgagtaata tgttttgaag cgcacgaaac
     9961 tagacccgat caaaatcaaa tttttttcgg gttgattcaa ggatttgcgt caataacgga
    10021 ttgttatttc tctatcacac tccttggtgg cgtatagagt tggtgtttac gtagtagcgt
    10081 atccaccaga cgcgtaaatt ttcttgcggt taggacgagt gcacgtttgt gttgatgctt
    10141 tggaacctcc ttatatttac tttgataaaa agctttgtat tcaggaagat aattttttac
    10201 agagttggca gcttcaacta agtagtatct aaaatatcga tttccttgtt ttgtcagagg
    10261 tgtattttgc gactgatagt ttccagattg gttcactttc caacttaatc cagcgtattt
    10321 tgccaatttt gtctgatctt caaatctttc gatttgacca atttcagcga ttaaaccggc
    10381 agcatagact ttaccaacgc ctggaatact ggttaagcat tgatattctg gaatgacaac
    10441 aattacttgt tcgatggctt tatctaattc tttgatattt ttttcaagtg ctcgtatttc
    10501 tcgtacgaga acacttaaaa caacattaat tgattcttgt gcaagagcac ctaggcggta
    10561 actcatggta attgcacgtt gaattgcttt agcgattttt tctggttgct tgaaacggcc
    10621 ttttcctttt tcttgaagta aatcacagaa agcttctaaa ggaagctcag caagttcatc
    10681 caatgtgaaa tcttccgtca tgagtgaaat gagggttgca ctaaaaagac tcgtcgattc
    10741 gtctcgcatt tctcgagcaa gcgtattaca tttgtacgtt agattttcaa gaaaatgttg
    10801 ctttgtacga atcaattgct taataagttg atagcgagta c
//
LOCUS       DAHHZJ010000101        10719 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.102, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000101 DAHHZJ010000000
VERSION     DAHHZJ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.102"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            368..1510
                     /locus_tag="NBL02_001951"
     CDS             368..1510
                     /locus_tag="NBL02_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCD2572631.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            1503..1709
                     /locus_tag="NBL02_001952"
     CDS             1503..1709
                     /locus_tag="NBL02_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCD2572632.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            1788..2981
                     /locus_tag="NBL02_001953"
     CDS             1788..2981
                     /locus_tag="NBL02_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCD2572633.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(3152..3889)
                     /locus_tag="NBL02_001954"
     CDS             complement(3152..3889)
                     /locus_tag="NBL02_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCD2572634.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(3891..5393)
                     /locus_tag="NBL02_001955"
     CDS             complement(3891..5393)
                     /locus_tag="NBL02_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HCD2572635.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            5652..7646
                     /gene="uvrB"
                     /locus_tag="NBL02_001956"
     CDS             5652..7646
                     /gene="uvrB"
                     /locus_tag="NBL02_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HCD2572636.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            7656..10472
                     /gene="uvrA"
                     /locus_tag="NBL02_001957"
     CDS             7656..10472
                     /gene="uvrA"
                     /locus_tag="NBL02_001957"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCD2572637.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
BASE COUNT         3489 a         1965 c         2323 g         2942 t
ORIGIN      
        1 aaaaagaaat acctatctga ttaaatccgt agatatgaat atcggttgaa atggaacttg
       61 ttagaaaagg caaaacagaa cttgctgaca aagtatttct gacacggtgg cggaagcctc
      121 tcaggcttac cgccatgaca ccttgtgtca aggggaagaa atgctttgtc ggctggcgag
      181 cctgcgagcc ttgtctgtgc ccccgttatc taacaggggg gcactatttc acaaaagaca
      241 agtcaaaaac catacaatcc caaagttgtg agtagtaatg agaagtttta agaagatagt
      301 gcatatcaca ctttttgaat agaggtttac aggtatttca aggctattgt gggattggag
      361 gaatacaatg aacaatttag aatttgcact tgaaatgaag aacaaacgtc ttgcaagcgg
      421 tctttcacaa ggagaacttg caagtcttac ccatgtatca agatatagta tcaaccgctt
      481 tgagaacgga aaagccaacg ccagcaagga aacacaaaat attatcctgc gttgtctgaa
      541 ttattatatc tgcgataagc ctttttatct gctcgttgat tatctgtctg tccgttatcc
      601 aacgactgac gcattggaaa ttatccgaaa ggtgcttggc atgaaagcag attattttat
      661 ccattatgac tatgggtatt atggctataa agagcattac gcctacggag aaatcaaggt
      721 catggcttct gatgacgaac acatgggcgt attcttggaa ttaaaagggg caggctcacg
      781 caacatggaa tatgtcttgc aggcacagaa cagagattgg tattcatttt taaaccgctg
      841 tcttgactgt gacggtgtta tccgacgttt tgacttggca atcaatgata tgtgtggtct
      901 gttggatatt cccgttttgt ctgaaaaata cagaaatggc ggtgcggatt gtcgctgtaa
      961 aaattatgaa aatgtgcagg gtggaaaact aagcggaaaa aaccgtaatt tagcaagcac
     1021 cctttatatc ggttcaaagg caagcacaaa atacttctgc ctgtatgaaa aacaaaagga
     1081 acaggcaacc aaaaagaaac atacggatat tatcaatcgt tttgaaattc gtctgcgtgg
     1141 tacaaaagca gtacaggcag ttgaggaatt gttattgaca tataaccctc atgggctggt
     1201 gttttacctt attactgatt ttgtgcaatt tcccgactat ccgctgtggg agatattcat
     1261 ttcccatgac agtttacctt ttgaaatgaa ccccgtacct gtcaatatgg aacgcactct
     1321 gcaatggctg gaaagacagg tcatgccgtc aatcgtgatg atagaggaaa tcgacaggct
     1381 gacaggctca aactacatga aaatgattga tgaatgtacc cacctctctg aaaagcagga
     1441 aatgcttgtg gaacagatgt gtacggacat agcagacgta atcgaaagtg agggggtgtt
     1501 ttatgagtaa tgcacaggat attcccgtat gggaaaaata cacccttacc attgaagaag
     1561 cgtcaaagta tttccgtatc ggagaaaaca agttaagacg attggcagag gaaaacaagg
     1621 acgctggctg gctcattatg aatggcaacc gcatacagat taaacgccga cagtttgaac
     1681 aggtcattga taaattggac gctatctaat gcaaatgagc cttgtatgtg ttatgatgaa
     1741 cacaagtcat atcaaggctc tttccaacaa ggaaaggagc agacaccatg aaagaaaaaa
     1801 gacgggataa caaaggtcgt atcctgcata ctggagagag ccaacgaaca gacagaaaat
     1861 acttatataa atatgtagac gcatttggaa acacaaaata tgtgtatgct tggagattga
     1921 cacccacaga cccgacacca aagggaaaac gggaaaaacc ctcacttcga gaactggaac
     1981 agcagataag acgggatatt gaggacggta tcgacagcac tggcaagaaa atgacgcttt
     2041 gccaacttta tgccaaacag aacgcacaga gggcaaatgt gaagaaaagc acacagaaac
     2101 aacgggaaca actcatgcgg ttattgaaag aggacaagtt aggtgctagg agcattgata
     2161 caatcaaacc ctctgacgct aaggaatggg cgttacgcat gaaagacaaa ggcttttcct
     2221 acaataccat taacaaccat aaacgctcgt taaaagcgtc attctacata gccattcaag
     2281 atgattatgt gaggaaaaat ccctttgatt tcaagttgag tgaagtccta gaaaatgata
     2341 ccaaaaagaa agtcgcattg acagaggaac aggaacaagc cttactctca ttcatcaaga
     2401 cggacaatgt gtatcacaag cattatgatg atgtgctgat actgttaaag acaggacttc
     2461 gtatttcgga actgtgcgga ctgacagtag ccgacattga tttcaagaat gaagttgtga
     2521 ttatcgacca ccagttacta aagagcaagg aacagggcta ttatattgaa acgcctaaaa
     2581 caaaaagcgg aataaggcaa gtaccattaa gcaaagaaac gatacaggca tttcaacagg
     2641 ttatgaaaaa acgtccaaag gcagaaccat ttgtgataga cggacggggc aacttcctat
     2701 ttgtcaatca taagggcaag cccaaagttg cgattgatta taacgcctta tttatccgta
     2761 tggtaaagaa atacaacaag caccacaagg acaatccatt gccacatatc acaccgcata
     2821 cgctacgcca tacattctgc acaagactgg caagcaagaa catgaaccca aaagatttac
     2881 agtatatcat ggggcattca aatatcagta tcacaatgaa ctggtatgct catgcgtcca
     2941 tagataccgc aaaatcagag gttcagcgtc taattgcata gaagtattta ccacgatttt
     3001 aaccacgctt gatagtgaaa atataagaag atagacctag atatgtgagg tttaccacaa
     3061 aagcaaaatg cccgtagagc ctataaaata aggctttgcg gacatttaag aagatataaa
     3121 aagatagtca aaaagacata tataatttat tttaaatatt taatacttta ttcaagaaat
     3181 cttttgtccg ttcattttgc ggattttcaa agatttgttg aggcgttcca tcttctaaaa
     3241 agtttccgtc gtcaatgaac atgacgcgat tggccacttc tttagcgaac cccatttcgt
     3301 gcgtaacgat aaccatcgtc ataccttgtt ctgctaactt tttcatgacg ttcaagacat
     3361 caccgaccat ttcaggatcg agtgcagaag taggctcatc aaaaagcatg atgtcagggt
     3421 tcatcgctag agcacgagca atagccacac gctgtttttg tccaccagaa agcatttcag
     3481 gatacatatc ttttttatct tctagaccaa ctgtctgcag taatttgatt gcttttttct
     3541 ctgcttcttc tctagattct ttcttcaggt ccatcggtgc taaaatgatg ttttccaaaa
     3601 tagaaagatg agggaatagg ttaaagtgtt ggaataccat tccgatatgt tgtctcacca
     3661 agttgatatc tgtgctttta tccatcaagt gggcgccgtc aataataatt tcaccgtcag
     3721 aaggttcttc taaacgattc aagcaacgca agaatgtact ttttcctgaa cctgaagggc
     3781 caatgataca gacgacatct ccttcgttga tggatacatt gatgtcgttt aatactgtgt
     3841 tatcgccgta ttttttgact aaatgctcaa ctaagatttt ttctgccatc ttacttcacc
     3901 ttcttttcta aaacttttgc taattttgtc aatgctgtga tcaaaatcaa gtacatgatc
     3961 gcaatgatca agtaaacata cgtactttgt gttgtacgtg caacaatgat cttaccagtt
     4021 tgcagcaatt cgactacacc gattgcggaa atgatcgttg tgtcttttaa tgagataacg
     4081 aactgattga cgaatgatgg gatcatgatg cgtatcgctt gcggcaaaat gattttttgc
     4141 attgtccgat tataagaaag acctaaactt ctggaagctt ccatttgtcc gacaggaacg
     4201 gcgttgatcc cgcctcgaac aatctctgcg atataagcac ttgcgttaag agtcaaggtg
     4261 ataacaccag caaggaagtc aggaatcgta aatccaacga taccaggcaa cccaaagaaa
     4321 ataaagaacg caagaaccat cattggaatt cctcggatga tatcgacata aatactagca
     4381 attgtgcgaa gagcgcgtat tggtgctaca ctgaataatc caaaaatcac accgattact
     4441 atagccaaag caaaggagat caaagttagg agaattgttc tccaaagtcc tttcaacaat
     4501 gcttgatagt tgttttggat aagtccaaga aatgttgatt catcagcagt ggaagactca
     4561 ctggaagttt ctgaaccatc cgctacatat tctttaataa tcttatcgta ttcgcctgtt
     4621 ctttttaatt ctttcaatcc ttcattgaac atattaagaa gctcaggatt ttgtcctttt
     4681 ttaacggcaa atgcatattt tccgccagat tcttttttga tcggtgtttc caattcttgt
     4741 ccttgtttga tcgcataacc gattaccgga taatcatcca tcattgcatc gattgcgcca
     4801 acttttaacc cgtcgtacaa ttgatcagca gcatcaaaat atttgataga atagccatat
     4861 tcagattcat ggtcgcttaa aaattcagca ctttcggtac caacttttgc tccgactgtt
     4921 tttcctttca aatcttcata agacttgatc gaagtatctc cttttttgac agctaattgg
     4981 atcccgctat caaagtaaga atcagaaaaa tcatagcttt tctttcgttc atctgttaca
     5041 gtcattccgg caatcattcc gtctgcttgt cctgattcca aagcttgcaa agcagcagag
     5101 aaaccacggt agtccattcg gatgttaaat ccttgcagtt tggcgatgtt ttccatcaat
     5161 tttacatcga tgccttcgta ctcgcccttt gcgttttgaa attcaaatgg agcaaaagca
     5221 gagtcactgg caattacgta tacgtctttt ttgggctcta ttttttgcat tgtctcgctt
     5281 gtgcttgact ctgccgttgt agtgttgtca gcttgtgcaa catgaccaaa cgcaagtgtc
     5341 acaagtcctg tcattaagaa aacaaatgag tgaaataaat gctttctttt cataatcaac
     5401 ctcctaaatt tccaatattt tattattata ggcgaaaagt gctgaatcag acaagactgt
     5461 aagctcgaaa aaatgaaaaa aatgcgagtt tctaacattt tatgttataa aacataacat
     5521 gtataataag ataaaaaagt ggcgaaagta tgttcggtgt ggtataattt tttagtaagg
     5581 aatgcctttg ctttcctttt ctttttattg gtcgtaaaat gaaaaggaat gaatgaaaag
     5641 gaggacgaag aatgattgag agagatacct cccgacactt tgagcttgtc tcgaaatacc
     5701 aaccggctgg tgatcagcct gaagcgatta accaattagt ggatggcgta gtcggcggga
     5761 aaaaagcaca aatcttatta ggtgcgactg gaacagggaa aacatatacg atttccaatc
     5821 ttattgagaa agtgaataaa ccaacactga ttattgccca taacaaaaca ttggctggtc
     5881 agctgtacgg ggaattcaag gaatttttcc ctaacaatgc tgttgaatat tttgtttctt
     5941 attatgatta ttatcagcct gaagcttatg tgccttcaag cgatacgtat atcgaaaaag
     6001 attcaagtgt aaatgatgag atcgacaaat tacgacattc ggctactagt tcattattag
     6061 aaagaaatga tgtgatcgtc attgcatccg tttcatgtat ttttggatta ggctcgcctt
     6121 ttgagtacca aaaacaagta gtttcgattc gacagggagc agaactagat cgcaatcagt
     6181 tgattcgtga cttagtcagt atccaattcg aaagaaatga tatagatttt cagcgcggtc
     6241 gttttcgagt gcgcggtgat gtcgtggaga tttttccggc ttcaagagat gaacgtgcct
     6301 tacgtgtgga gttttttgga gatgaggtcg aacgtatccg agaagtcaat gcactgacag
     6361 gtgaagtttt aggagaaacg gaacacgtag caatcttccc ggcgactcac tttgtgacga
     6421 atgatgaaca tatggagcat gccgtagcga atatcaaaga ggaattagaa cagcgtttaa
     6481 ctgtcttgcg aaatgaaaac aaacttctgg aagctcaacg tttagaacag cgaaccaatt
     6541 acgatattga aatgatgtta gaaatgggct acacgtcggg aattgaaaat tattctcggc
     6601 atatggacgg acgtaaagaa ggtgaaccgc cttatacgct attggatttc tttcctgaag
     6661 attttttgat cgtggcggac gaatcgcacg taaccatgcc tcaaatcaga ggaatgtata
     6721 atggtgaccg agcgagaaaa cagatgctag tagattacgg tttccgtttg ccatctgctt
     6781 tagacaatcg gccacttcgg ttagaagagt ttgaaaaaca tgtcaatcag atcatttatg
     6841 tctctgcgac gccaggacca tatgaacatg aacaaacaga taccgttatc caacaaatca
     6901 tccgtccgac agggctgctt gacccagtga tcgaagttcg tccaatcatg ggacaaatcg
     6961 atgatctagt aggcgaaata aatgaacgag tcgaaaaaga ccagcgggtg tttgtaacga
     7021 cattgacgaa aaaaatggca gaagacctca cagattattt caaagaatta gggatcaaag
     7081 tcaaatattt gcatagtgat atcaaaacgc tggaacgaac agaaatcatt cgagatcttc
     7141 gattaggtga atttgatgtg ttagttggaa tcaatttgct tcgtgaagga ttagacgtac
     7201 ctgaagtttc ccttgttgct atcttggatg cggataaaga aggcttttta cgtagtgaac
     7261 gttccttagt ccaaacgatt ggacgagctg ctcggaatga agaaggtaaa gtaatcatgt
     7321 acgcagacaa agtaacagat tctatgcgtc tagcgatgga tgaaacgtca agacgtcgta
     7381 cgattcagca gaagtacaat gaagaacatg ggatcgttcc aaaaacgatc attaaagaaa
     7441 tccgagattt gatttctatt acgaaagaat cggaagatga tacaaaagaa gcggtacaag
     7501 tctcgtacga agaaatgacg aaggaagaaa aagacacgtt attgatgaaa ttagaaaaag
     7561 aaatgaaaga tgcggcaaaa gctttggatt ttgaaacagc cgcgaatgtg cgagatatga
     7621 ttttagaatt aaaagcttca aagtaggagg acaacatggc taacgataaa attacgattc
     7681 atggtgcacg tgcccataac ttgaaaaata tagatgtaac gattccacga gataaattcg
     7741 tggtcgttac aggtttgtcc ggttctggaa aaagttccct agcatttgat acgctgtacg
     7801 ctgaagggca gcggagatac gtagagagcc tttctgctta tgctcgtcag tttttaggac
     7861 aaatggataa gccggacgta gatagcatcg acggattgag tccggcgatt tctattgatc
     7921 agaaaacaac aagtaaaaat cctcggtcga cagtaggtac tgtgacggaa atcaatgact
     7981 atttacgctt gttgtttgca cgtgtaggac atccgatttg tcccaacgat catatcgaga
     8041 ttaccagtca atcagtagat caaatggcag atcaagtaat ggctttgcct gagcgcacaa
     8101 agatccaagt gttggcaccg atcgtggtaa agaaaaaagg acaacataaa aaaatattcg
     8161 aacgaatcca aaaagaggga tacgtccgcg ttcgagtcga tggtgagatc tatgatttaa
     8221 gtgaagtgcc tgagctggag aaaaacaaaa aacatgatat cgcgatcgtc atcgaccgga
     8281 tcattgtgaa agaagggatt cgttctcgcc tatttgattc tttagaggcg gccctgcgat
     8341 tagcagacgg ctatgctctg gttgacgtga tcggtgaaga agaaatgttg tttagcgaac
     8401 actatgcctg tccttattgc ggatttactg ttggcgaact agaaccaaga ctgttctcat
     8461 tcaatgcgcc ttttggagct tgtccagact gtgatggttt aggtgtcaaa ttagaagtcg
     8521 atcaagattt agtgattcca gatagaacga agacacttag tcaaggtgct atcgttccat
     8581 ggaatccaat cagctctcag tattatcctc aaatgttagc gcaggcttgt gaaagtttcg
     8641 gtatcgattt agatacacca ttcgaagaat taccggaaga gcatcaggag atcatcttga
     8701 atgggacaga tgatcttttc catttccatt atgaaaatga ttttggtggc gttcgcgatg
     8761 tagaaacgac tttcgaaggg gtattgaaga atatcgagcg tcgttatcat gatacgaaca
     8821 gtgatttcac gcgtgagcaa atgcgtcttt atatgacaga attaacttgt cagacatgta
     8881 aaggttatcg attgaatccc caagctctat ctgttcaaat caatggaacg aatatcgggc
     8941 aaacaaatga gttgtctatt caaaaagcag tagatttctt ctcaaactta acgttatctg
     9001 aacaagagaa agtaattgcc aaaccgattt taaaagaagt aaatgacaga ctttcattct
     9061 tggaaaatgt gggactagac tatctaactt tgagtcgcgc ttccggaaca ttatctgggg
     9121 gagaagcgca gcgaatcaga ttggctacac aaattggatc gaatctttct ggagttttgt
     9181 atattttaga tgaaccatcg atcggattgc atcaacgcga caacgatcga ctgattggct
     9241 cattaaagaa aatgcgtgat cttggcaaca ccctcgttgt agtcgaacat gatgaagata
     9301 cgatgtgggc agcagattat ttgattgacg tcggacctgg ggccggagaa caaggcggac
     9361 aaatcgtggc tgcaggcact cctgaagaag tagctaatga tgaaaattct ttgacaggga
     9421 aatatctttc agggaaaaaa tcaatccctg taccaaaaga acgccgtaaa ggtaatggga
     9481 aagcaatcaa gatcactggt gcttcagaaa acaatctgaa aaatattagt gttgaatttc
     9541 cattaggaga atttgtagca gtcacaggtg tgtctggttc tggaaagagt acattggtca
     9601 atagtatctt aaagaaatcg ttagcgcaaa aattaaataa gaattctgct aagccaggta
     9661 aattcaagac aatttccggc tacgaaagta tcgaaaagat catcgatatc gatcaaagcc
     9721 caatcggccg gacgccgaga agtaatccag cgacttatac aagtgtattt gatgatatcc
     9781 gtgggttatt tgctcaaacg aacgaggcaa aaatgcgggg ttataagaaa gggcgtttta
     9841 gtttcaacgt aaaaggcggt cgttgtgaag cttgtcgcgg ggatggaatt attaagatcg
     9901 aaatgcactt tttgcctgat gtctatgttc cttgtgaagt atgtcatggt aaacgatata
     9961 attctgaaac attagaagtg cattacaaag gaaaaagcat tgctgatatt ttggaaatga
    10021 cagtagagga tgctgtagaa ttcttcaagc acattccaaa gattcatcgc aaactgcaaa
    10081 cgattgttga tgttggctta ggttatgtga cgatggggca accagcaacg acattgtccg
    10141 gtggtgaggc acaacggatg aaacttgcca gtgaattgca caaaatctct aatggaaaga
    10201 atttctatat actagatgaa ccaacgacag gacttcatag cgatgacatc gcccgcttgt
    10261 tgcatgtatt acaaagatta gtagatgctg gtaacacagt tttagtgatt gaacacaatc
    10321 tagatgtaat caaaacagca gattatatca ttgatttagg accagaaggt ggagaaggtg
    10381 gaggaacgat ccttgcgact ggaacaccag aagaaatcat taacgtaaaa gaaagttata
    10441 caggtcacta tttgaaaaaa ataatggtat aaagaggata gatagctgag atagttaaaa
    10501 aaacataaaa aaatgaattt ttgcttgttt tattaatatc agtgttctag aatcattaca
    10561 tggtagttct ttctacactg attggtatag ataaaaactt cctataagtt caatccgttc
    10621 aaaaaagaat tgagcaatcg gaggactttc ctttgattgc ttttttcttt atggtatgat
    10681 attccttgtg tagttggaaa agaaaaacaa gagattatg
//
LOCUS       DAHHZJ010000102        10691 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.103, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000102 DAHHZJ010000000
VERSION     DAHHZJ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.103"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            69..659
                     /locus_tag="NBL02_001958"
     CDS             69..659
                     /locus_tag="NBL02_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCD2572638.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            751..2256
                     /locus_tag="NBL02_001959"
     CDS             751..2256
                     /locus_tag="NBL02_001959"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HCD2572639.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(2340..3692)
                     /gene="celB"
                     /locus_tag="NBL02_001960"
     CDS             complement(2340..3692)
                     /gene="celB"
                     /locus_tag="NBL02_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HCD2572640.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(3692..4210)
                     /locus_tag="NBL02_001961"
     CDS             complement(3692..4210)
                     /locus_tag="NBL02_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572641.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(4226..4552)
                     /locus_tag="NBL02_001962"
     CDS             complement(4226..4552)
                     /locus_tag="NBL02_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HCD2572642.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(4565..6544)
                     /locus_tag="NBL02_001963"
     CDS             complement(4565..6544)
                     /locus_tag="NBL02_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCD2572643.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(6565..6879)
                     /locus_tag="NBL02_001964"
     CDS             complement(6565..6879)
                     /locus_tag="NBL02_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HCD2572644.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            7047..7340
                     /locus_tag="NBL02_001965"
     CDS             7047..7340
                     /locus_tag="NBL02_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCD2572645.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(7418..8530)
                     /locus_tag="NBL02_001966"
     CDS             complement(7418..8530)
                     /locus_tag="NBL02_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCD2572646.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            8917..9096
                     /locus_tag="NBL02_001967"
     CDS             8917..9096
                     /locus_tag="NBL02_001967"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572647.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            9290..9460
                     /locus_tag="NBL02_001968"
     CDS             9290..9460
                     /locus_tag="NBL02_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCD2572648.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(9884..10624)
                     /locus_tag="NBL02_001969"
     CDS             complement(9884..10624)
                     /locus_tag="NBL02_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCD2572649.1"
                     /translation="MTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGL
                     TTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTP
                     GGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVET
                     QAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGE
                     TIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         3334 a         2151 c         1825 g         3381 t
ORIGIN      
        1 ggtttcgttt ttttggtaaa gtaaagaaat atacagcctt ttatggctaa catttatagg
       61 agatatcaat gacacaagaa ataaaaaaac tacccgttat atttttaggt cttttcttta
      121 tcacagttgg attgaattgg ttcttgctcc ctcacgatat agcttccgcc ggagtgggcg
      181 ccatcggaca tttagttgaa acggcctttt ctatccaacg ctcagttact gtttggagca
      241 tcaatcttat gatgctgctt ctagccgctg tcctattagg aaaatcagta ttttttaaga
      301 ctgtagcggg aagtctgctt tttcctgttg ttttagagat tgttccaaaa gtggaaatga
      361 tctcctcaca tttcttttca ctgatttcag gaagtctttt gttcagtctt ggggtttaca
      421 cattgtacac agtcggggcc tcaaatggcg gtataacgat accgccgatt attttccaca
      481 aattttttcg gcttccaatg ccgcaaggac ttttacttac caatagtctt atcgtctttt
      541 tgaattatta tgtttttgga ttgttggaga cactattcgt tctcctatct attacagtca
      601 gttcattgtt tatgaaaata ctcatccgct tgtcccctgt gtcaagaaaa tttgaataaa
      661 gctggatttt gtcatagcgg aaacaaacga tatattaaaa agagtgtgct ataataaacc
      721 aagaaattga ataaagcgag ggatcaatca atgagcttct ctttagaaga catccgtcat
      781 ttactattaa aagaaaatct attaaaagaa tttgtttcaa caagaggctg gcatcttgat
      841 gtaccagaaa aagctgaatt tcttcagtta agttatgatt caagaaaagc tgatgcttct
      901 actttgtttt tctgtaaagg aatgaatttc aaagaagaat atttggattc agcaatcgaa
      961 cagggtattc aatattatgt atcagaacaa ccatacgaaa attcagcagt aggaatcatc
     1021 gtaacggata tccgtaaagc aatggcgctt cttgctatgg ctttttacga ctatccgcaa
     1081 aataaattga aagtgatcgg attcactgga acaaaaggta aaacaactgc tgcgtacttt
     1141 acaaaagcaa tcttggatca cacaacaaat aaaaaaacag cattgctttc tactatgaat
     1201 acaacgttag atggcaaaac atatttcaaa tcccatttga caacgcctga gtcattagat
     1261 ttatatcgta tgatgtcaga agcagttgaa aatggtatga ctcatctagt gatggaagtt
     1321 tcttcacaag cttacaaaac acaacgcgta tacggcttga ctttagatgt cggaatcttc
     1381 ttgaatatct cgcctgacca tatcagtccg atcgaacatc cgacatttga tgactatttc
     1441 tattgcaagc gacaattgat cttgaattca aaaacagttg ttttaaatca tgaaagtgac
     1501 tattttgatt tacttaggga aacagccgat ctattccaga ttccaacgat cacttatggt
     1561 cgttcggaag aaagcaatta tcaagtgatc cgttcagata aaggtactca cggttttact
     1621 ttatcttctc atgaagatcg cttagctatc tgcgatactg cttatgatat tttgctggct
     1681 ggaggattca accaagaaaa tgcagccagt gcgatcattg ccgcagcact aacaggtgcc
     1741 tccagtcaag atgcacaaga cggattaaaa gaagctcgtg taccaggccg aatggatcaa
     1801 ttgatccaat ccaatggagc gcatgtctat gtggactatg cacataatta tctaagcttg
     1861 aaaacattgc tgaaatttgc caaaaatgaa catccagatg gccgtgtaat tgttgtatta
     1921 ggcagcccag gaaacaaagc aatctcccgt cgacatgatt ttggaaaagt gctatcagaa
     1981 acagctgaca tcgccttttt gacggcagat gacccagcat ttgaagatcc gaaaaaaatc
     2041 gcggaagaaa tcaatgaagc aattactaat ccagatttga tcgttcatta tgaaatggat
     2101 cgtcctgaag cgattcgacg cgctttagca gaaagcgctc cgcaagattc agtcgttatt
     2161 gcgggaaaag gcgtggatcc ctatcaaaaa atcaatggtg tagatgaacc ttatgaaggg
     2221 gattatgcaa tcgtgaagcg gttgattgaa gaataatggt atttatatac tagtggtttc
     2281 ccatttctaa gaaaaaaagg gactgtgatt ttttgtcaca gttccttttt tcttagaaat
     2341 cattttaact tttcactagc taattctgct gccttttctc tttcttcatt taataattta
     2401 ttatcataga atttgataaa tgggaaccag ataatgaatg cgatgattgc acaaatgact
     2461 gctaacacag cagctttcca atcaccacca ctaccaacaa atgctccgat cccgactggt
     2521 gaaggccacg gcatctgtgc aagcatcggt ttgacgattt ccagccgaat ggcaaaataa
     2581 gcgagtgtag cagaagccat cggagctaag aaaaacggaa ccgctaaata gggattgtag
     2641 acgattggca tcccgaaaat gattggttca ttgatattga ataatcctgg tacaagtgag
     2701 gcttttccta acactttcag ctgatcagat ttcgcgagga atgcaataaa gatgatcaat
     2761 cctaaagtcg cacctgatcc accaacggtt acataagaat tgttgaattc tcctgccaac
     2821 ggataatttg cgccttgtgc gttagctgcc atattagcta atacgatcgg ggtcaagaaa
     2881 gaagtgatga tattcgctcc atggatccct acgatccaca atgcatggat caagaaataa
     2941 atcaccatga caccaagcca tgtattcgtc aactgcgtaa caaacccaaa tgggatagca
     3001 atcattttga agatatccgt tcctagtgca actaataatc cattgatgat catcaccaca
     3061 aacgcaatga caaatgttgg gatcagagca gtgaaagaac gagaaactcc tggcggaaca
     3121 acatctggca tttttatgac ccaatttctc ttgacacaca tacaatacaa ttcgaccgca
     3181 ataatcgaca taataatagc tgtaaagatt cctgaagttc ctaaacgttc gacaaaagag
     3241 cccatcctaa taccatttac tacagtttct gtctcattga tagaagcaat caagctgatt
     3301 ttaccatcag tcatgatcaa ttcagggata cacataaaga atgcaaaaac agatagtaat
     3361 gcccccgtga gcggattaac atttaatttt tgttcatctc gttcaattga cgttaattca
     3421 taagcaaaca caatggcaaa gtaaatagat aaaatcccca tcgtcattgt attgacgatc
     3481 atgtacaagt cactaacttt gaaaaaagtt gcattaaaaa atccttctaa aaatgggaac
     3541 ggtaatggca aaatatttaa aaccaaaaac atcgagccta caatcgtgaa aggaatagaa
     3601 gccatccccg ctgccatgac agcccgaacg attttaagtt gcgcaatttt ccccataggt
     3661 cccatcaggt atttctgcaa aaaatcaaac attagttatt ctcctttcct aaatataacg
     3721 ctttttcata ttgtttaata cggatatact gttggtcttg gttgagtgaa attttcttta
     3781 aacgatactg tataaatgca ctggcaaaaa taccgcttac aacaagaatg aaaacaaata
     3841 actgtcctcc attgttattt gccataaaag gatacaagaa tgtaaatcct gaggtaaaca
     3901 acactggaat cactaataaa ttaaagacac tctggaacaa aaaataccac tttgtccatg
     3961 gaacgttcgt ttggtgtttt cgataaagct ttacttgttc tcctactgca ggcaaaatag
     4021 ctaaaatcag aatcatcggt aaactacttc ctaaactttt agctgataga agcagaatca
     4081 tccattgcat gttcatgaaa aagaaaaagg cagtcaggta acgtacgatt agataacgat
     4141 tgaaatacat gtttttcaaa ctaagttgct ttttttctaa ttcgattttc ctttttactg
     4201 tatcctgcac aatgatcctt cctttctatt tatttttttg atacaaataa ttcatttcct
     4261 gtgcaacttc caataatgtc atcgtcgtca tcagatgatc ttgtgcatgg accatgataa
     4321 tttccatttc tatttttgtc ccgctggcat attctttcag aagttgagtt tgtgactgat
     4381 gcgcatgaag aatctcttca tttgcttgtc gcaacttctc ttctacttct tcaaacatct
     4441 ctttacgcat cgagcgaaat gcttcatgta tcagtgtccg tgcatttccg ctatggaaaa
     4501 tgatatcaaa agaagtcact tgtagtgttt cactgtttat tttttcagcc atctgttttc
     4561 ctccttattc gatcagtttg ataaatttac tgctgaaatt ctcaaaaata ggttcagcta
     4621 agatttcctg ctgtactttt agctggtcaa ctaacctaac gattgcttta gtcacgaccg
     4681 taattccttc attttcgaca taagaaggcg aaactaaaaa aacaaaatga atttcttgat
     4741 attgatccca ctgcatccca gaaggaatca atgcaacggc tacctttgtt tcgattccaa
     4801 ctggaacagc gggatgtggg actgccactg tatcactgaa tatgatttgt cccatatttt
     4861 cacgttgctg gatctgcttt cgcatttctc ttatatatcc ttcttcttca tttatgctca
     4921 gcttttgcag caaatcatga agaatttctt cctttttcgg ttcgtacgta taagtcttga
     4981 aaaatttttc agacaactgt tcggtgtaaa tctgcttttt ttgctgtaga gaaaatgctg
     5041 gtatcggtct atctgaggaa tgatagggta tagattctcc aactaccttt cgtattttac
     5101 gaacatcatc ttcattcaaa aaaatactga catgtaaaac tggaattttg aaaaacattg
     5161 tcgaaagatc aatagaagaa ataatcaggt cgattccttt taaagttgtt tcattgatct
     5221 cataatagcc ttttacactt accactgtaa tatttttacc gaattcactg actacgcgat
     5281 tctttaatag ttgggcactg ccgtaacccg ttgcacagat aatcaatgca tgtagtttat
     5341 gcgcagctct ttctttttcc aatgccgcca tgagatgcaa agccaaatag gcccattcat
     5401 cttcattgat ggtatagttt ttcaaagccg gcatgttgct gaaatgcctt tttactgcgt
     5461 caaagacttt gggattttct ttctttatct cctcagtcaa tgggttctca atgcgaatac
     5521 ctcggctcaa tcgaatcatc atcggtgaca aatgatcgag tagtccattg aaaagctgga
     5581 gatcctctac taatgaaaaa tgaaattcct gagacaatct tttcagaact ttcgctaatt
     5641 cctttgatag ttcctgatca ttttgatttt ctttgtgatt cgacttagcc atgagatgta
     5701 atgtcaaata agcaatttct tcttcgggaa acgatacacc tgcgattacc tcaatacgtt
     5761 gaacgatccg ctgcgccact tgatactcta ctcgcttaga aacttcttgt tcgatgccta
     5821 attccttgat ttcaaaccct tctcggagcc gtttgatccc caaagccaag tgtaaagtca
     5881 gattctgtat gataaaatcc gaaactttta acttagcttc tcttgtttca tcgagaatga
     5941 taatgactag ctcttcaaaa ctgatgtctg ggaaaaaacg gctttgccct aaatattctt
     6001 tcaatgaatt tccataatgg tttccaaaaa aagtatccat gataaaatgc cgtcgatttc
     6061 tttcttcccc ttcgatccat gtcccttgac catgcttaga aacgattttt agtgaatatg
     6121 gaatcaatcg ctccttgatt tcttgcatgt cctttgtcaa actggaacga ctgatataaa
     6181 gctcttctgc caaatcatcc ataaaaagaa tggcatcttc caaaagcaat tggttcaata
     6241 tatacttttg tcgatcttcg gactcataaa atccttgcgg ttctctagca tatccaggta
     6301 ctatttcttg ccgactcaaa aacaagtcaa atgtcagtct ttgtaaaata tgcagttggt
     6361 agccataacc tggtttagca atgatcttac ctccgttttt ttctactaac tctttgatcc
     6421 gctgcaagta attacggatt gtccgatcag ataataacaa ttcagaagct aaacgttgac
     6481 tggtgacata ctccttcttg tgatcaatca aatatctcaa cagcaatttt tccttctgtt
     6541 tcattatcat ccactcctat tccatcatat gttctcttcg atcacctctg ctaacttctg
     6601 gatgcctgta gggattggga catacgcttg gaaagggata ctaacaacag gctttccaac
     6661 agattctcct aattttttga atttatccaa aaacatcgtc gtttgcgggc tgatcagaaa
     6721 taaatcaaaa tcactattct taatcatatt atcgccttct gtagctgaaa ctgcatctac
     6781 gagtacctct tctctttttt gcttaaagaa atccgttgtc tttgaggcca tcatcgagga
     6841 tgacattcct gccgcacaaa taatcaatgc ttttttcata aatcaatcac tccttttatt
     6901 tttataaacc aagcatatct tgtaagcgtt ttctattcaa aatatatatt tccgtagcat
     6961 tgcggaaaaa atgtgtatca tatcttgaat aataatttct ggcaccgtga tatactagtg
     7021 atagaatttt ttcaggaggg gatttagtga gtatcacagt gaatattttg tatacaagta
     7081 caaatggaca agccagaaaa tttgcagaag aaatggtcga aagaaacatt gtcaaagcga
     7141 ttcgtgcaga agaaggaaac gagaaatatg actattactt tccactagaa gatccagaat
     7201 ctcttttact gatcgaccgt tggaaagatg aagaagcaat cgaaaagcat cacaaatccg
     7261 aaatgatggc tcaaataaac gaactccgta aaaagtataa attaaaaatg aaagtcgaac
     7321 gtttcactga tttaccgtaa aaagatacac atggaatgat agcaatttat catccatgtg
     7381 tatcttcact ttttccttat tttttattcg atctcatcta atgattcaaa ccgtgttttt
     7441 ctcttatttt tcggataagt atttcagtca ccccaatata catgattcct aaacataccc
     7501 caaggatatt ctcaaaaatg cggatcgtta ctgctcctgg aaccccgaat aaactggcag
     7561 tgattgccaa agcaccaaaa gaattaaaaa ctgttttata tctataggta gaacaaacac
     7621 ctaaagctag accacctaat ataccgaccc agttaaaagg aataaattgg ctgataccaa
     7681 taaataaaac acatccaatc aatgagccaa tgattcggtc tactgctcgt tctttgaaac
     7741 caatcgacat caatccatag ctagatacaa tagaagaaaa tgcaaaccct gcccacatca
     7801 atcgctgaaa agggacatac tctcctgcaa cgatcaaaag actaatgcca aatgcataga
     7861 tgagcatcca aattttttcc tgcgaataca tgcctttcaa aaaaatctct ttaaacaagc
     7921 ttttgtcttt gttcttttct cgatgttttc tgtaaaggag aactgaaaac caacaaaaaa
     7981 acaaaaataa taagctactt gtttgtgtaa agaaattctt gttcaacgaa tctttaggta
     8041 acgaataaac aataaagaga tatgaaaagc cgtacaaaga agcattcccc attttgggat
     8101 cactagcggt tgccattaat atgctgctta gtaaaataaa atgaatcaac atgcccataa
     8161 ctgaccattt gatcagctgg acatacggtg ctactaatag tgagaacatg acgattgcta
     8221 aacctatgat cgaatgggaa atcttataac caaagtcaac aaaacgaata ctcatcaaca
     8281 tacagaataa aaccacagcg tatggtttat gcttcgattc aaataatgtt gtaattataa
     8341 tgataaaaca aatggcaaac aatacgatga ataatgatcg taaaaacaat gaacaaatat
     8401 acatgcactt ttctttttta gaagctgctt ctcgtatttt ttgttttaaa ataaacggat
     8461 ctagttgtag agactgataa aaatccacct gttgtcgtcc tttcttttta cttttatttt
     8521 ttacagtcat ctatgacagg ttgtaaatac tatggaacat catatggcgt tgtcattttc
     8581 ttgtcaacta tttttcctat tcatgttttt ataaaaaaac atatcgtttt cttgtatgat
     8641 taaatcacca caaaataaac aactgagaga acgatatgct ttatgaatgg ttctatcaaa
     8701 aaaattctga gatcaataga caaaaatttg atgattacag aggtctctta cgaaacctta
     8761 cagaagaaaa aaacattgat cgttgctgca gttctcttcc ctgctccatg tgtctgtaga
     8821 aattgtggtt ctgctgtggt agatggaaat aggaaattaa tggtggtgaa aaacaagaag
     8881 aaggaaacca ttgtccactt tgaataatat aatcatatgc caatggttat acgcctaaaa
     8941 aagcagcgct atacctgtaa aaactgtcga agccattgga acgctcaaag ttattttatc
     9001 cgtccaaggc actcgatttc aaatcatgtc agacataaaa tcacttcttt actgactgaa
     9061 aaagtgtcgt tattttttat ttcgaaaagt tgttaggtat ctttgacaac cgttattcgc
     9121 acattgaaag agttgagaag ctatttacca aagtcttcca aaagaattct gtcttgagtg
     9181 ttgagggttg tgaatttcgt tctcacgttt ccgttgagga taaactgagt tttatttccg
     9241 cggatggcga gacagaaaaa ttaatcgaga aaatttacct acacaaaaaa ttcctcgatt
     9301 aacaaactat ttccttagct gcactaatcc taaagaaata gaattcttag ttacagacat
     9361 gaacgccacc tactttcagc tcaccaaaca tgttctacca aatgcgaaat tggtgattga
     9421 tcgattactt ggtttttcca cgcctttcaa ggaggcgtaa cctttattca cgagttggtt
     9481 gaagctttcc gagacaaaag acccgacttg tttttctcct tattgacaaa gagccattaa
     9541 aactagagaa aaaattacaa ctgatcgctg attatttgta gaaagaaaat caaagtatta
     9601 cccaataaac ttgagtcatt aggcaaaaaa cactgcgaaa ctcaagaatg attttcgcag
     9661 tgtttttgta tattttttat taactagtat tactacaaag gtttattttt cacttctcaa
     9721 cgcagcacaa aattgtcttt acttatttac tggttgggtt caaccttctc tactccgata
     9781 ctaatatttg tttgtatacc ccttctaaaa tcggtagttt ccattcaatt gatgggtact
     9841 agttgatttt aaaaactatg cttattcaat tttttatttt agctcatata tttcgtttct
     9901 ttctaaattt tatactgatg aaaacaagta gcacacccaa tattgacaat ataaactggc
     9961 taatgatggt ttctccagtt tttggaagat gcttatttcc actgcttgtt ggattgtttc
    10021 ctcttatatc tgagctatca gcaacttttc tattctcttt tgttaaaaag atcgccactt
    10081 cttgttcttt ttcgatcttg aaagttactg gtgtcgcatc caattcatac cccgttggcg
    10141 cttgagtttc taccagctga tatgctcctg gtgctaagcc atctatcgcc agttttccat
    10201 ctgctcctgt ggttagacct gactgcagaa cggttccatt ggcatcttgt aactcaaaaa
    10261 cagctccttg caggacttct ccgctttggt catcgatctt tgtcaacacg acaccgcctg
    10321 gtgtaagtcg attttctttc gtcaattcta ccaccgctgt ctgacttctt tcgatctcaa
    10381 actcgattgg tgtcgcatcc aattcatacc ccgttggcgc ttgggtttct accagctgat
    10441 acactcctgg tgctaagccg tcaatcgcca gttttccatc tgctcctgtg gttagacctg
    10501 actgcagaac ggttccattg gcatcttgta actcaaaaac agctccttgc aggacttccc
    10561 cgctttggtc atcgatcttt gtcaacacga caccgcctgg tgtaagtcgg tttgctttcg
    10621 tcaattctac caccgctgtc tgacttcttt cgatctcaaa ctcgatcggt gtcgcatcta
    10681 attcataccc c
//
LOCUS       DAHHZJ010000103        10663 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.104, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000103 DAHHZJ010000000
VERSION     DAHHZJ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.104"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            335..2281
                     /locus_tag="NBL02_001970"
     CDS             335..2281
                     /locus_tag="NBL02_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCD2572650.1"
                     /translation="MIERHVNLLKLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLED
                     YVVNYQVTIERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFG
                     DKPVSLTHLSQSFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAI
                     KQLVFYYANDMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLIST
                     LVIQCNRLLKGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLC
                     RQLFAHRVTNNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLR
                     LKKGIKIQNPLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIE
                     DQSKANNIIIICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISS
                     LSFDAGGIPLVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKF
                     VQSSLVKTGVQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALP
                     HANPSMVIESNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQK
                     EMVDRLLQVHDSEKLLRIFQQKGE"
     gene            2284..2739
                     /locus_tag="NBL02_001971"
     CDS             2284..2739
                     /locus_tag="NBL02_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCD2572651.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            2753..4081
                     /locus_tag="NBL02_001972"
     CDS             2753..4081
                     /locus_tag="NBL02_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCD2572652.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            4115..4399
                     /locus_tag="NBL02_001973"
     CDS             4115..4399
                     /locus_tag="NBL02_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCD2572653.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            4401..4916
                     /locus_tag="NBL02_001974"
     CDS             4401..4916
                     /locus_tag="NBL02_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCD2572654.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            4932..5594
                     /gene="rpe"
                     /locus_tag="NBL02_001975"
     CDS             4932..5594
                     /gene="rpe"
                     /locus_tag="NBL02_001975"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCD2572655.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            5601..6173
                     /locus_tag="NBL02_001976"
     CDS             5601..6173
                     /locus_tag="NBL02_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCD2572656.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(6360..7880)
                     /locus_tag="NBL02_001977"
     CDS             complement(6360..7880)
                     /locus_tag="NBL02_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCD2572657.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
     gene            complement(8123..8308)
                     /locus_tag="NBL02_001978"
     CDS             complement(8123..8308)
                     /locus_tag="NBL02_001978"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HCD2572658.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            complement(8292..8474)
                     /locus_tag="NBL02_001979"
     CDS             complement(8292..8474)
                     /locus_tag="NBL02_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572659.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            8715..8933
                     /locus_tag="NBL02_001980"
                     /pseudo
     CDS             8715..8933
                     /locus_tag="NBL02_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            9428..10027
                     /locus_tag="NBL02_001981"
     CDS             9428..10027
                     /locus_tag="NBL02_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCD2572660.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            10091..>10663
                     /locus_tag="NBL02_001982"
     CDS             10091..>10663
                     /locus_tag="NBL02_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage abortive infection AbiH family
                     protein"
                     /protein_id="HCD2572661.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIGD"
BASE COUNT         3526 a         1674 c         1990 g         3473 t
ORIGIN      
        1 attaggactc aattgttcat agatgagcat ttgtaattaa gttatattat aataaaaaaa
       61 tacgaatttt agtgacgcca ttatattgtt tttatttttc aattgtaata tttcatatta
      121 cttccataat aaggagtatt ttctattgga atatcttagc tcttttaagg atttctcctt
      181 ttttgtttct cattttttaa gattatttgg atttccttta caacaaactt ccacaagtat
      241 tattgacata gatgtaattg agggaagggg gttccagtgc tagaataaat aatgaaaaga
      301 ggtaacaaat gctagcgtta agaggtgaac aagtttgatt gaaagacatg taaatttatt
      361 aaaattatta agtcgacaag aagactttaa acccgcttct ttttttagtg ataaattggc
      421 agtgtcgacc aagaccattt atcaagatat tgaattgttg gaagattatg tagttaatta
      481 ccaagtaaca attgagcgat taccttcttt gggtatcaaa ctcataggtg ataggaaaaa
      541 cgttgaacat ttgatttcat cggtagaaaa ttatcttgaa aaagatattt attctccaga
      601 aaaaagacgt gtcgagatca ttaaagaagt tttgtttggt gataagcctg tttctttaac
      661 tcatttgtct caaagtttta tggttagtaa aacatcgtta tacaatgacc tgagaattat
      721 caataaaatt attggcagtg gagaagttaa gttgcagtca tctgcagaag gaattagttt
      781 tactggtaat gaacaagcaa ttcaacgagc aatcaagcaa cttgtattct attatgcgaa
      841 tgacatggaa tcaaaaacat tttttcaagt tttaaatatg ttgtttgatt caaaagttat
      901 tagtgtcatt catgatttgc tattcaatga ttacagtgaa ctgactgaaa aggtttctga
      961 ttactatgtt cggtcattga tttctacttt agttatccaa tgtaaccgtt tactgaaagg
     1021 tcatcattta actgttgaag aagaattcct cttcaatagc attcgctata tggaaacgta
     1081 cattgtggca aatggtttaa ttgaagaatt gaaggcaaaa ttgaatcttc cattttatga
     1141 cgcagataaa gaatatctat gtcgtcaact atttgcacat cgtgtgacaa ataatttaaa
     1201 aaccagcaat gaagagtacg ctatgagcgt tcataaattg attgaaagaa tgagtgagat
     1261 tgaaaaaatt gatttgactc aaaatgatcg attatatcaa tccttattgt atcatgtgcc
     1321 agcaatggtc cttcgtttaa aaaaaggtat taaaattcaa aatccgttat tagaaaatat
     1381 caaatctcaa tattcagaac tctttagcat agtatggtac gccatgtcca tccttgaaga
     1441 agaatttgat gtgactttaa cggacgatga agtttcctta attttgattc attttcagat
     1501 tgctattgaa gatcagtcca aagcaaataa tatcatcatt atttgccaat acggtatttc
     1561 ttctgcgcaa ttaatttaca ataaagttcg acggttttta ccagcaaaag ataatgtaga
     1621 aatatcaact ttagataagt ttcatttagc tgaaattggg gaagttgatt tgattatttc
     1681 ctctctttcc tttgatgcag gtggtatacc cttagtaagg gtatctccat tggtaagtaa
     1741 aacagattat atgaatatta tggaagctta tactaaatat ttaatagaag aaccttcgcg
     1801 atttattcaa aatgaactga ttcctaaagc ggttaaagtt cccaccatat ctaaatttgt
     1861 tcaatcatct ttagtaaaaa caggagtaca ggtggaaagc aaagaagaat gtttggattt
     1921 gatgatttcc gaactagaag ccaattcatt agttactgaa gagtttcgtg cttctatctt
     1981 ctctagagag aaaatgggga atactaattt agaaagtggt gttgctttac cgcatgcgaa
     2041 tccatcaatg gtcattgaat ccaacatttt tattgtagca ttaaaaaatg cgattaattg
     2101 gggcgatcga aaagtcaaat tagtcattat ggttagttta gctgaagaag atgtatctga
     2161 gattagagga gtatatgaag aactttacca atttattggg caaaaagaaa tggtcgatcg
     2221 cttgttacaa gttcatgata gcgaaaaatt actaagaatt tttcaacaaa aaggagaata
     2281 aaaatgtatc tagataaaga attatgttta tttcaattag aagtgaaaga tcaaaaagaa
     2341 ttgtttcaag taatgtctga acaattaaaa aacgctggtt gcgtgaaaga tagtttctta
     2401 gaaggtatca ccaaccgtga acaagaattt ccaactggat tagaagtaaa tcaaattggg
     2461 tttgctatcc cacatacaga ttccgcacat gtaaatagct ctcaaatctg ttttgcctca
     2521 ttaaaggaac cactggtatt ttcagatatg acagataaat ctcatgaaat acctgtacgt
     2581 ttagtcttta tgttggcaat gtcacaacct catgaacaaa ttgatacact acaaaactta
     2641 gttagcctat ttcaaaatga agaaaaagta aatgaattac ttgcatgtac cacgaaagaa
     2701 gcatttattg agttagtaca ttctgcagga gtttattagg aggaatagag agatggatat
     2761 tgtattaaat gcctttgaat ggtttattgg attagggtca aacgtctttt taccactgat
     2821 tatttttgta atcgggatat tatttggatt aaaacctggt aaagcatttg tttccggcgt
     2881 tactgttggt atcggttcta ttggtttagg attagtttta agtttattat cagatggttt
     2941 aggaacagca attcaacaaa tgggggaaca atacggtggt ggcctgaata tcctagatat
     3001 tggcgtaggt gttggtggac ctttggctta ctctacatca ttgggaattt taatgattcc
     3061 aatttcatta attcttaact tcatatttgt ggttttgggt tggacaaaaa cattgaatgt
     3121 tgatatttgg aacttttggt tcgcaatttt cctaggtatg attactcaat ctgttacggg
     3181 aaacttttgg tttggcttaa ttggcgcgat tgtcgcagta attttacaat ggttcttagc
     3241 agatcgtaca caaaaaactg ttagcgaatt tttcggctat ccaggaatct ctatcacaca
     3301 tatgatggga ttatcaggag tactatttgc caaaccagta aattgggttt ttgatcgtat
     3361 tcctggattt aataaaatag atgcagatgc agaaacatta acaaaacgtt tcggtgtttt
     3421 aggtgacaca gttgttattg gcctaattat cggtattgta gtgggtatta tggcaggcta
     3481 tgacgtagca ggaattggaa acttaggaat tatgactgcg gcagtgatga agattatgcc
     3541 taaaatggta gcaatgttta tggaaggttt aatgccaatt gcagaagcgg cgaaagaatt
     3601 tacgaataaa aagttaaatg gtcgccaagt aaacattgga atggatgcag cgttaactgt
     3661 gggtcatcca actattatgt caacttctct attgcttgta ccaatctcat tattgctagc
     3721 actaattctt ccaggaaata aagtattacc ttttggagat ttagcattct ttgctttcgc
     3781 catctgttta atgattcctt attttaaagg aaatattgtg agatcattag taggatgttc
     3841 gttgtatgtt gttacaacat tatatatggc aacatggttg gcaccaatta tcactgatgt
     3901 gtttacatta gcaagtttcg atgtggggac agcaggatta gtaactatgt tgttatgtgg
     3961 attatggcca gtagggttgt ttgttttatt agcatcttac tttggtgtaa ttggaatagg
     4021 tggattagga gttgttttag taggtgcttc tatctacatg aacaaagtga agaaagctta
     4081 atcataaaat taaaataaga aagaagagat caatatgaaa aaattattag ttatttgtgg
     4141 agcaggtcat gctacttcta caattgcagt agcaaaatta tcaaaatggt tggaagaaga
     4201 aggattaaca gatcaagtga aaatttatca atctaaaatt gctgatgagc taaataaaat
     4261 tgatgaatat gatgctgtag taagtacaac aattgtccct gatgtaatta aagctaaagt
     4321 aacgaatggc ttgggcttat taacaggtat tggtacagat gccatttatg cagatgtaaa
     4381 agaaagatta ggtgtttaat atgcgttttc atcatgacac tttgactatt gaaacagttt
     4441 cagggcgtcc ttcgtatcat gatattcgaa aagaactttt agatacagtt gatacagcag
     4501 gtattgagaa tggaatgtta gtaatttcga gcccgcatac aacttgctca ctcttttttg
     4561 aagaaacgat gcatgacaca aacttttttg gtgatgatta tttacatgta gacatcaaca
     4621 atgtaatgga aacgattatt ccaaaaatga cgagtgaaaa tcaatacaac agtccggggc
     4681 caaaacacat tgattttgga acaagattat ctgatcccaa ttaccctgca gaaaaatggg
     4741 tcatgctaaa taccgatgca catttacgtt catctattta tggaaccaac tcaatgactt
     4801 taattgttca agaaggtaaa cttttattag gagaaatggg ccgtgtctat tttgtcgatt
     4861 gggatcaact aagagagcga actagaacaa tcaatctcat gattatggga gaataaacgg
     4921 gggaatagag aatgacaatt gctccatcat tgttaaatgc acctatcgca gaagttaaag
     4981 agttatttga aatcttaaaa gaaaagaatg ttgattattt gcatattgat gtaatggatg
     5041 gctgttttgt accagatatg gcttttggac caagtgttat taaagatatt cgtccactaa
     5101 cagacgttgt gtttgatgtt catttgatga ttgatacacc agaaaaaatt gtcacaagat
     5161 ttgtagaagc aggagcagat attgttacct tccacattga agctaccaaa cacgcaatga
     5221 aggtcattca gttagtcaag gaagcaggag caaaagtagg gattactttt aatcctgcaa
     5281 caccagtcag ttttattgaa ccagtattat ctttagtcga tcaagtatta gtaatgacaa
     5341 tcaatccagg aacagagaat aagcatttta ttcaagagac agtagttaaa atcgaacagt
     5401 tagatgtaat acgcaaacaa aatgattata cgtatgatat tgaagtagat ggcaagattg
     5461 ataatcaaac gattaaagtt tgttctaaag caggggcgga tatttttgta tcaggcggtt
     5521 tcatcttcaa tgggaaaaat accccgcaag aacaaattga taaactaaat caatcattag
     5581 aggaagtgaa gtaaaagatc atgaccaata aagaccaaaa taaaaactat ttagaagcga
     5641 ttatcttaga attacaaaat aactcaaaac gtattaattc tgtagagtta gaacaattta
     5701 gtgagaaaat tgttcaagct aaacacatct ttctagctgg tgcaggacgc tctggaattg
     5761 ccatgcaagc atttacgaat cgcttaatgc atttaggttt ttcagtcagc cttgtcggtg
     5821 aaattagttc accacattcg caacctggag atttattaat tatctgttct ggctcaggtg
     5881 aaacaggtag tttaaaaaat ttagcggcca aagcaaatca aagtggtgta agcattgctt
     5941 tagttaccat gaaaaaagac tcgtcgattg gtcaattagc agattctgtt cttgtattac
     6001 caggtattac caaagatgaa aatcaacgag aagaagatac tttttcacaa cctatgggtt
     6061 caagttttga acaattagca tttctcacct ttgatagttt agtgttatct ttaatggata
     6121 aaacaggtga aagctcagaa acgatgtttg ctcgacatgc agattttgag tagtagattt
     6181 taaagataaa aaaccattct ctaaatgcta attattcagc aaatatgaga atggtttttt
     6241 tatttgatta gacataactt aataattcag tagttttgag cgtaagcgtg tcatagaatg
     6301 gtacctaaac acaaatagcc cgatttgata cactcaaatc aggttatttg tggcttatat
     6361 tatttacaga tttcttgtat ctgctgattc catggcaaat aagcgcgtag cgcttcaggt
     6421 gtcatcgaag acagattggg aatctctgta aaaagatgat ttaaatattt gcggatgttt
     6481 aacccattcg caacagctgt ctggataata ctcaatataa ttccatttga tctagcgcct
     6541 tttaagcttt tagaaaatag ccagtttttt cgaccgatca ctaactcttt gatggcacgc
     6601 tctgctcggt tatttgaaag ttccaagcgt ccatcaagca acacaatctg aagtccgtct
     6661 ttatgattga ctgcgtattg aaccgccttt ccaagctgcg attttggcaa ataagcaaaa
     6721 ctctcacacc aatgaaaaaa ttcagtcatc acaggctgca acgtttcctt cctcgctaca
     6781 aacctttctt ctgcgctaag cttattaaag gtccgttcga ttcgaaacat cttgtcacag
     6841 aagtctaatc ctattttggc atgacttttt ggggacggtt gtttacccaa tgcttcaaaa
     6901 aactttctgc gaacatgcgc ccaacaataa acattgttta ccggcagctt tccataggcc
     6961 gcgtaaccat cagtatgcaa gaaaccgcta aatgattcta aaaaactttt tggctcatca
     7021 acggaacgcg aatttgcgtg ctcgtagagc acaacttgtt tttcttcgct tttacctgaa
     7081 gcatataacc agtaaaaggt cttttcttta tgagtattca atacccgata gcttgtctca
     7141 tccgcgtgta agacctcctg attgcttaat tcttgatgaa ggcaattgta gattggctct
     7201 aaaaccattt cacttacctt gatatgccaa ttagtgattt ttttacgatc aagagggaaa
     7261 ccaagtgcat gccattcttt ttcttgtcga tatgccggca cctttttaac aaatttttcc
     7321 caaatactct gtgtcacaat tgaagaggaa cctagactat tattgatcaa cggtttagga
     7381 acaggcgctt ttttgatttt tgtttctcct aatgcttcac attctctgca atgataggca
     7441 tgttggtaga tgacttgttt ttctatatgg gcaggaacat acaatggctc ttctctaagc
     7501 cttgtcgcac cgatatcatg taaagtatga ccacagtctt cacacaaaca atcggcacct
     7561 tttaattcat aatgaatagg aacttcaggc agaccttcta atgaaagtgc tttgagtccc
     7621 ttgcgttttc tgcgtttaaa agaagatacc gtaatgggtt cctggctttc ttgtccagtg
     7681 tgctctggtt cattaaaagt tgagtcgtct aataaatcga tttgcccatc tacgggtggt
     7741 gtttcttttt ttcgtccaaa caagcgtcga ttcatttcag caatttgttc tctaagaaga
     7801 gtattttcct gttgctggat ttcaagcaac tgtgttaatt gcttgatttg tacaagaagt
     7861 tcagattcca tttttgtcat ttttacacca ccattctagc gattttctta catctattat
     7921 accattttat gacaacgaaa aacagacaaa atccttgatt taacaaggtt ttcgtctaat
     7981 atatcgcacc ttttttcgtt tcttttagcg tacatttttc ttcgattgaa aggccagtca
     8041 gtaagacttc ttttctggaa cgcggccatt gaaggtaccc actttcaaat cttttgtaca
     8101 ttaatacaaa tccattttga tcctagtaaa gagccttgaa gcgatctttc ttcgtaccgc
     8161 aaaataagaa aagcgcttga tcgtaaacat caaggtcata ttcttctata acgaaagctg
     8221 ctagtccatc aattcctttt cgaagatctg tttttccaca aacgataaaa atatgtttca
     8281 atgcagttgc atcaatgagc attggcattc aactctttta acaaggtata caaaagatat
     8341 ttatccattc cattataaat aaatatttcc ttatcttccg atttgattct gcaagctagt
     8401 tttggcttgg tgtcaatttg cgatttttga gccgcacttg aaaattgaac tggtacaata
     8461 tcttttttat tcattcgttt ttctccttct atatttgata gtttaattat agaagaaaga
     8521 taatcggcgt acgagatact atcctatgac acgcttacat ttaatttaat attttgcctc
     8581 aaattagcca cactttttat agaactaaga ggaaaaatga agttttcttc ctattaatat
     8641 agttgtttga aatcttaaat atagcgtttt acgatgtaat gggaagtatg gtgaataaat
     8701 caactatgtt cgtacgaagc atttactcaa ctaatttaat cgaatctttc aataaacaaa
     8761 tcaagaaata cagccaccgc aaggaacagt tccaaaatga agagtcgatg gaacgtttct
     8821 tagtctcgtc ttttgatact tacaaccaaa aattcctagg tcgcagtcat aaaggtttcc
     8881 aacaggccga aggcgagctc gaacaaatgc tgagtcaacc gatggagaat tagaaacaat
     8941 ctacagtagg gaagaaccat tgacacaaaa ttattgacgc tcccaactaa aagtgtcaaa
     9001 ataatgatga aatcatttag ttcttataaa ttagaaaaga gaaaataaat attccatatc
     9061 tactttattg gatccgtaac atgactaaat tctgaatact ttttaaagct tatcatttgg
     9121 taaaactgaa tataagttaa ttatatagac tgaaaatttt gaattttcag aatagaataa
     9181 cttaaaagta ctgacaaaat tttttaaagg ggcaatatta atgttctgag ataatcaaat
     9241 cctaccgaaa atatcaaaaa tccaatgtga cacaatcaga ttgtgtctca ttcgagggaa
     9301 tcacacaatc ccacgtggct ctaggcagaa ttgaattttt gcttggggct ttttccttat
     9361 attgattttg aatgagactt attatatgtt ataatgcaat taagtatcat tggagagggc
     9421 gaagaaaatg aagacggtag aacctattcg agacaaaaaa aagatcgatg cgatgaaggc
     9481 aatcttggct tcgggaaaat atggccaacg aaatttggtt cttttttcga ttgggatcaa
     9541 caccgcctat cgcatttctg atttgcgtca attgaagctg gccgacgtgc tggaaatttc
     9601 acgcggtcgc gtggtggtca aagatcgatt agcgatgaag gaacaaaaaa cagctaaaca
     9661 taattcggtc tatatttcca acaaattgcg aaaaacgatt ttggagtacg ttcaacagga
     9721 atttcctgat caactccttc agcaagattt tgacggttat ttgttcccta gtcgaaaagg
     9781 gacgaatgaa ccgctcaacc gccaaagtct gtggcgaatc attcatgatg ctggcacggc
     9841 aatcggattg aaagacattg gcccacattc catgcgaaaa acgttcggtt actttctcta
     9901 taaacagggg accaaaacag aaatcattca gtccttgttg aaccattctt ctcagcgcga
     9961 aacattgcgg tatattggta ttacacaaga agacaaagat acagctgtaa aaagcttaga
    10021 tttataatat aaaagttaag tttaaacatg gatagtttta aattagtaga ttgagtgttg
    10081 ggaggaaaga atgagtacat tatatattat aggaaacggt tttgatttaa gtcatggttt
    10141 aaaaactaga tacactgatt tttcaaaatg ggttgaaaaa agtcatagtg aaatattcga
    10201 taaaatagac acaatgataa atgaaaataa tgaggtgaga agtacagctg atgataagat
    10261 aggttggttt aattttgaaa gtagtttgca tgagttaatt caaagtccag tctttgaaga
    10321 ttatgtagaa gagtctaagg attatctcat aggttatgga catgaagatt ggtcagattc
    10381 tgcccatcat gattttcaaa atatggttta tgattatgtt gtttctctag aagaattaca
    10441 acaacttttc aaggaatgga tacgcaaaga aatacagccg acaatagaac ataaaattcc
    10501 aaaataccaa ttttcgtccg atgtactatt ctttacattc aattatacag atgttttgga
    10561 aattctatac aagcaaccga aagaaaacat atatcatatt catggcagtt atgacgatat
    10621 aataatcggc cactccgaga attttgacaa ctttatagga gat
//
LOCUS       DAHHZJ010000104        10548 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.105, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000104 DAHHZJ010000000
VERSION     DAHHZJ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.105"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(536..901)
                     /locus_tag="NBL02_001983"
     CDS             complement(536..901)
                     /locus_tag="NBL02_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572662.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(977..1222)
                     /locus_tag="NBL02_001984"
     CDS             complement(977..1222)
                     /locus_tag="NBL02_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD2572663.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1292..1546)
                     /locus_tag="NBL02_001985"
     CDS             complement(1292..1546)
                     /locus_tag="NBL02_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572664.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1710..2033)
                     /locus_tag="NBL02_001986"
     CDS             complement(1710..2033)
                     /locus_tag="NBL02_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572665.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            2220..2293
                     /locus_tag="NBL02_001987"
     tRNA            2220..2293
                     /locus_tag="NBL02_001987"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2254..2256,aa:Ile,seq:cat)
     gene            complement(2678..3175)
                     /locus_tag="NBL02_001988"
     CDS             complement(2678..3175)
                     /locus_tag="NBL02_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCD2572666.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(3215..4024)
                     /locus_tag="NBL02_001989"
     CDS             complement(3215..4024)
                     /locus_tag="NBL02_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCD2572667.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            4358..5215
                     /locus_tag="NBL02_001990"
     CDS             4358..5215
                     /locus_tag="NBL02_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HCD2572668.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            5208..6110
                     /locus_tag="NBL02_001991"
     CDS             5208..6110
                     /locus_tag="NBL02_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HCD2572669.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            6143..6493
                     /locus_tag="NBL02_001992"
     CDS             6143..6493
                     /locus_tag="NBL02_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCD2572670.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            6542..7465
                     /locus_tag="NBL02_001993"
     CDS             6542..7465
                     /locus_tag="NBL02_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCD2572671.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            7543..8436
                     /locus_tag="NBL02_001994"
     CDS             7543..8436
                     /locus_tag="NBL02_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCD2572672.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            8474..9037
                     /gene="amaP"
                     /locus_tag="NBL02_001995"
     CDS             8474..9037
                     /gene="amaP"
                     /locus_tag="NBL02_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HCD2572673.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            9051..9239
                     /locus_tag="NBL02_001996"
     CDS             9051..9239
                     /locus_tag="NBL02_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HCD2572674.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            9252..10154
                     /locus_tag="NBL02_001997"
     CDS             9252..10154
                     /locus_tag="NBL02_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCD2572675.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            10186..10428
                     /locus_tag="NBL02_001998"
     CDS             10186..10428
                     /locus_tag="NBL02_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCD2572676.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
BASE COUNT         3702 a         1833 c         2029 g         2984 t
ORIGIN      
        1 aagcgttatt attttagtct attttcaggg ggggaacctt atgataaaaa aactattttt
       61 actatcaata ttaacgctag caatggcagg ttgttctgac tctggcttag caactgaact
      121 gatcacaaaa tcaactgaag ccacgacaga ggagttaaaa tgtggaaacg atcacagtga
      181 ctacagaggc ttaaatcgac gatgcatggg aatattaggc agaagcaatt gtaagcatcg
      241 atgtagtaat cgaagaaggt gtagaagaag caacagaaaa atcaaaagaa agatcaaaag
      301 aagcaatctg tgctgtgcat cgtatacaga cgtcgaagat gcggtacttt taaaccacgt
      361 caggagatgt tcatttatgt ctagggaagt tggcgatgaa ccaaaactag agtacgcttt
      421 tagttttgca cgtgaggatg aagataaccc taaaacttac gacattttag cttgatctaa
      481 gatggttaaa gctttttaat gaagtaagag gagatagaag gcttttctag ttatattagg
      541 taagataaat tcctttttgc tccaaatttg ctaaacattc tgctagataa acttcatcgt
      601 gttccggata aatcggcacg ttgcgctcta cagtaggtaa atcaaaaaat gcttcgcatg
      661 atttgccacg atacatagga tttttccact ctggtgctgg gctgtaacca cgtttttcca
      721 tttcatccat gacaagctga tgatattgga ataatctata aggagaatag tgaaaaacat
      781 aatccacagt agcatgtttc tttccccagc catttcctct taatgcacag cattcccgat
      841 gttgtcctaa aagctgttga cgaggaagct ttgaaatcaa tttttcatgc cataatcgca
      901 tagaaatgtc tcctttatat atagattgtt aaccttagac gttaaatgaa aaaaccagca
      961 ccaaggctgg aaaaaatcac ttcagtttgc ttgctgtttc tgagcgacca attaggtaat
     1021 caacggaaat atcgtaaaag tcagcgatat agattaattc aatgatgctt ggaatttctt
     1081 ctccttcttc gtacttacga tatttgaata aaggaatact taacgcgcta gaaacagcta
     1141 cttgagataa agatcggcca gttcttaaga aattcagacg acaacgtaaa gtatccagta
     1201 aaatagcttc ttcgatactc atttaatcac ctttcattat agtagaatag ttataatgac
     1261 tatagaaagc tacacatgca atcaatagta ctcaatgttt tttatttaaa cgtatcaaat
     1321 aaagaatgac gatcaataaa gacaaaacca gtcctgataa ttcttctata aaaacactat
     1381 cgggttcaaa gtagttgtaa ttaaaaactt tccacataaa gtagatagcc ggaagagtca
     1441 aaatagctaa ataaaaccgt tctttgttcc aattcataag ataaacaata cttgcgacca
     1501 gtgcgaccgc aggcacccaa aacaaaactc tcaccaacgt aatcatgtaa cggactcctc
     1561 aacgattcat ataataaaag ataacatgtt attgcataca tcacaaatga aatgctagga
     1621 aaataaaaga agaatagatc aatgaaaaat gaaaaagcag aacagttttc tgctgataag
     1681 caggaactgt tccgctgaat gtgtcttatc tatcgagaaa aaatcaatgg attgatatct
     1741 aagtaattca atacatataa aaagaggacg atcgcattca ctaaaaagga aagaatgatg
     1801 atcccatagc gaatggaaac atatttaaca aatacttttg tttgaatacg ggcatatctt
     1861 tttcctttat agctgagcat catgacaaga agcaaaaggg taatcacacc ttgaaaaatc
     1921 attccaataa acagaattcc tgaggtttga taatttaatt ggagaaaata agctgctgcc
     1981 gcaactaaat cgaataaaat cgcaataaat ggcaaaataa tcactcctaa cattgagaat
     2041 aaataaaaga ataacatttt ttctaaaata agcaaataaa aattaaagta acaaaatagt
     2101 tatgacacaa gtaaaagaaa taagaataac aatattattt tacttatatt ttgaaacttg
     2161 tgagagtttg tcattcgtgc tatagttgaa aagtaacaaa aataaatttt atttatgatg
     2221 tacctttagc tcagttggtt agagcagacg gctcataacc gtccggtcgt aggttcgagt
     2281 cctacaaggt acataagagg ttgtgaaaaa gctgccctgc atcaagtaat aagaacagcc
     2341 aagtaaaaac agcttcttat gtttttactt ggctgttctt attactgcag atgcaagctt
     2401 ttgaataccg tttaaggaag aggttgtgaa atcagcgttt tgacctgagt attagcttaa
     2461 caaacggaaa aatagttcct catattttgc cgtttgttaa gctaataccg agggtcagct
     2521 gattgaacac catttaagaa agaggtcgtg agcctgccgt ttcactctgt gtcataaaaa
     2581 agccaatcta ctttgataga aaaagcacgt aggactaaaa cagttctgcg tgcttttttt
     2641 attgtgtgtt aaatatcgta aatgaagata gatattttta tgattcgacg atagagacgt
     2701 tgaaatcttt caacagctct aaagcggatt ctccgtgttt tctactggag actaacattg
     2761 gatcaacaaa aaagtctgct tcaggaaaag cactctgcag gcctatcaga ttagagagta
     2821 cacacaagtt tgtttcaatc ccgactactt cgatctcttt ttgttcttct ttgctttcaa
     2881 ataaagtttc ctggatttca gctaaagttt caggtggaat cgtaaagtaa tactttttaa
     2941 tcactcgttc attttcttgt ggagaaagaa gagggatcaa ttgcaaatct tctcgttctt
     3001 cttctttgtc cttcagttca actggtatat cccgtgtgaa gagaatatat tcattatttt
     3061 ggcgtgcttt agctaatctt tgagcaatac gttctgccaa ttgatctgac tctggaatat
     3121 aaaattcgct gtttggatca agaatatgat tttgcatatc aataataact aacattttct
     3181 ttcctcctac attcatatcg tcattttatt ctttttaata gaagtttttt aaaatataag
     3241 caacaccagc ttcttcattc gtcagtgtta cttggtttgc aatttctttt acttgtcgct
     3301 gtgcattccc catcgcaata ctcaacccgc tgtaattcaa catagcaatg tcgttttctt
     3361 gatcgccaaa agccatgatg tcactcgatt cgattccaag gtaatccgcc agaaaaacaa
     3421 gtgaatgagc tttagtgata cctgtaggcg tgatttctaa gaaatgcgga tcagagaata
     3481 ctgattggat accatctgct tgcagatctc tttgtagctt ctgtagtttt tcttcactct
     3541 tatctgttaa tgctaattta agatagctaa gagaaggatc ttccaacata tttaaaattt
     3601 gatcaaaaga atggttctgt aaattgaaat gttcataata agggtcatct ttgactagat
     3661 ttgattcatc gaatgtcagc tgatcatctt gggtttctcg gctagcaatg atggatattt
     3721 tcgcttcttg agcatagaag aaggcttttc tcagtgtatc gctatctatt tttgaacgtc
     3781 tgagaacttt cgccattttt ccatccttca tttcagcaat cagtgaacca ttggaagaaa
     3841 tcaaatagcc accgattcct aattcggaaa aaatatcttt agcagaaatg ctgttccttc
     3901 cggtagcgac ggttacgatt ccgccatttt catcaaattt ccgaagtgct tggatatttt
     3961 cttctggtat ctttccattt cgatcaagca aggtaccgtc taaatcaatc gctgctagtt
     4021 tcattttatc ttcctacttt ctttttacat atctattttc ctgttacctt aagattaaat
     4081 gagaatttaa aaaaatgaaa ctaacttgct ttgtcattat gaattttttc aaaaaacatt
     4141 cttaagaaaa aggtggctat atatagtagt ttttggtcaa tacctttgaa taaaaagcca
     4201 tgaaaaacga atagttagcg cttaccgatg cgttttgtga taaaaaaatt ttaaaatatt
     4261 caatgaaaac aaagcatata ttttgcttta aaaccaaatg aggaatattc tagatgtaga
     4321 acaggttaga aaaagattag aaaaaggagt gattttgatg gagaagagat acggcgtagt
     4381 tcctgtggta actgcttcag acgaaaatta tgctccttac ttgagcgtga tgatcgcaac
     4441 tgcattagaa aattgcaata aaacaagacg cataaaattt tatgtgattg acgatggtct
     4501 atcagaatat agtaaagaag gattggaaga aacggtaaat aaatattcaa gtaatgcaag
     4561 tatccaattc ctgacagtgg aaaaagatat ctacgaagac ttcctagtca gtgaccatat
     4621 cacaactacc gcttacttaa gaatttcttt accaaactta ttagctaaag aggattataa
     4681 gaaagtcctt tacttagatt cagatgtgtt agtactagac gatatcgtaa aactatatga
     4741 tgagccgtta aatggaaaaa caatcggcgc tattatcgat ccaggccaag tgaaagcttt
     4801 ggaaagatta ggaatcgatt ccgatgactt gtacttcaat tcaggagtga tggtgattga
     4861 cattgatcaa tggaataaaa aagaaattac agaaaaaact atccattacc tcagtgaaaa
     4921 tggcgatcga atcatctatc acgaccaaga tgctttgaat gctgtacttt atgaagattg
     4981 ggaacaactt catccaaaat ggaacatgca gacttcgctg atttttgaac gtcatccagc
     5041 accaaatgaa aaatatgaac gtcagtataa agaaggaaat gaaaaacctt cgatcgttca
     5101 ctttacagga catgataaac cttggaatac gttgaaagat catccgtata ccaatttata
     5161 cttaaaaaaa ttagcacaca gtactctaac gaaagtaggc gagatcaatg aataaaaaag
     5221 aaatagcagt agtcgcaagt tgtaacacaa aattcgtacc acatttagct gcattatttg
     5281 tctcagtatt agataattgc aacccgtcta aatttgttcg gttctatgtg attgacgatg
     5341 atatcgattt tgaaagtaaa cagcttttac gcttctcagt gaaaaacgca cgaatgaact
     5401 cagatgtgga attcttgaaa atcaataaag aattctttac aaatgtggta atcagcgatc
     5461 gtattccaga aacagcttat tatcgtattg cgattccgga attgttccgc ggcacagaag
     5521 ttgaacgtat cttatatatg gattgcgaca tgatcgcttt acaagatatc tctaaattat
     5581 ggcgtcttga cttcggtgat tcaatcgtag cagctgttga agacgctggg ttccatcaac
     5641 gactggaaaa aatgaaaatt tcagctaaat caatgcgtta tttcaactca ggtttgatgc
     5701 tgatcaatgt gaaaaaatgg ttagatgaaa atattacgca aaaagtatta gatttcatcg
     5761 aacataatcc agaaaaatta cgattccatg accaagatgc attaaatgct atcttacatg
     5821 accgttggtt accacttcat ccgcgttgga atgcacaagg ctatatcatg gcaaaagcaa
     5881 aaaaacatcc aacagcagca ggtgaacgtg aatacgaaga aactcgcaac aatccttaca
     5941 ttatccattt ttcaggacat gtgaaaccgt ggagcaagga ttttgaaggg ccaacgaaga
     6001 aatattacga aaaatatgca gggatgacgg ctttccgttg tgtagctaaa ttccctaaat
     6061 atccaaaata tgcaaaagtt caaccaaaaa cagatgtagt tgttaaataa tagaagacaa
     6121 caactgaaga gggggagtag gaatggaaat tacaatgtct aaaaatgcag tagagacgtt
     6181 aattgaaaag gttggggaaa atacaaaaat cgcactcgcg cttatcaatg attccgatcc
     6241 atttctaagg gacaaaggtg cttttgcaaa aggcagcttt tttcaaatca ttccttttgt
     6301 ttcggaattt ggagaatatg cgactaaaat cgagcatcca ttattggaca tctatacatc
     6361 taaattagag caaaattatt tcggtaaacg attgaacatg gacttcaaca aacagcttga
     6421 cagcttttca ttagaaaatg aaatcgcagt actcgattat aatatcaaat taaagaactg
     6481 cttcttcagc taatagtttc gagaaagtcc aaaaaggagg tatacattga gatactactt
     6541 tgtggatgaa gaaaagaatg taatagaaac agatgaaaaa aaatataatt ggctcgtact
     6601 tgatagtgca aagcccgaag agattgagca agcaatcgaa gaattcgagc ttcctgatga
     6661 tattttcgta ggaaccgaat atccggaaga agtttctcgg atggagcgcc tttatgagac
     6721 gaaactagag catccatttt ctttggtagt gattaatcta gatcagtcag ataatcgtat
     6781 cgaaaaaaaa ttaacgccaa tatcttttgt actttccgat aatctcttga ttacttgtat
     6841 cgatagaaaa accgaatttg tggatcggct gctgacaaag catggaaatc atttaaactc
     6901 gtttgaaaag atcatcacgt atacattatt agatatttac acccattacg tgaaagaact
     6961 aagagagatg aaaaagcgaa ttgacgcttt ggataaagca gctcgtaaaa caacggaaaa
     7021 tgaagaactg tataaacagg cagatcttga aagagacatc gtatatatcg atcatacctt
     7081 aagagatcaa cgggaaacaa tggatcaatt atggaattct gaaactttca tcaaacgttt
     7141 agacgatgaa cagctactct atgatgtcca tttgagacag cgacaaacag aaaaaatgat
     7201 cgagatctat cgagatttat tagagagtat tggtgatcta ttcaacggga tgatggacaa
     7261 taatctaaat catatcatga aatatctaga ttcagctgca ttaatcattt ccgttccggc
     7321 gttgttcgca gggatttggg gaatgaatac aggaggatta ccgggtaaaa gttctgctct
     7381 aggctttata ctcgtagtct ttggctcttt gatagcagca gtagttttag gttaccattt
     7441 ataccggaaa gattatacaa attaaagaat ctatttaaaa caggtatcaa acaaagaaat
     7501 ttgttttggc aatacaattt aaaaactagg aggaatacag caatgacaga accaaaatta
     7561 atcgacccgc gaaacttgta ccatacggag aaatttccta aacaagatca ggatacacca
     7621 gctttacagc agaatatgat tcctgttcct gattgtggag aagaatccta tgaaggcaat
     7681 aatcaactag aaaaccgccg tgtactaatc actggtggag attcagggat cggtcgtgca
     7741 gcagcgattg catttgctag agaaggggca gatatcgctc ttcaattctt cccaggagaa
     7801 gaaaaagatg cagaggaagt agcaaaatac gtgaaagttg caggacgtaa gattgtctta
     7861 ctaccttttg atcttcgtga cgaacaagca cctaaagaaa tcatagataa agccgtgagt
     7921 gaacttggcg ggctagatac gatggtacta aacgcaggac agcaaatttc ttgtcagtct
     7981 attgctgagt taccacttga gcaagtcaaa gacacattta tgataaacat catcgctatg
     8041 tttgctttag tgaaagaagc tgttccgcat atcccagcag gaggagcaat cgtaacaaca
     8101 acttctgttc aagcattcaa tccaagcgaa catttattag attacgcagc aacaaaagct
     8161 tcaatcgcta actttacgat cggtttagca aaacagcttg cacctaaagg aatccgtgta
     8221 aatggtgtag cacctggtcc aatctggaca ccattacaac tagacgaagg acaaccttct
     8281 gattcgatcc ctgaatttgg tcagcaaagt ttactagaac gtgcaggtca gccaggagaa
     8341 ttagcaccag tttatgtttt cttagcatcg aataaagcaa gttatgttac agcccaaatt
     8401 tacggagtaa ctggtggaga agcgatcaac ttataagaag ttgatcgttt cacccctatg
     8461 aggaggtgcg aaaatgaata aaggtgtaaa aacgatcgga gtgatcggaa cattactttt
     8521 gctatctgtg cttttgtttg cgatactatt aagtgcaacc tatccattgc cttttcagct
     8581 agagaagttt cgattctttt ccatcactaa ttcatacgtg caacaatatg tattttggct
     8641 tgccgttgca tttgctattt tgacaattat cacgttactt gttttcctgt tttatccaaa
     8701 aacaatcgga aactttctac tgaaacatgg tgatggggag ttgacgttgg ataaaaaagc
     8761 aattgaagga atggttcgtt ctcacttgca tgaagatgaa ttcatccatt caccaaaagt
     8821 aaatgtcaaa gcaacgaaaa acaagatcgc cgtcaatgta aaaggggagt tgaaacgaac
     8881 ttcagcactc attggtaaaa caggaacatt gatgaaagaa atcgaaaatg aagtacaaca
     8941 catattggga acaaaagagc cagtgaaagt atcggtgaaa tacacaggct ttgaagaacc
     9001 agctaaacga agcaatgacc gcaaacgagt agaatagggg gggaccaaaa atgaaagaac
     9061 tattttcgac atacaagctc cctatcatct ttggggtact tggattgata ttagccgttt
     9121 tatggatcac cgtgggcttt ttcaagacct tattgatctt gatcatggca gcgatcggcg
     9181 tcgctatcgg tttttatctg aaacaaacga aaatcttaga tcactatttt aaatcttagg
     9241 aggaaacatc catgaataac aataacaaaa ataataacaa taaaatgaac aaccaaaaac
     9301 cagtgtctgc acatccagac ccaacgacac caaaccaatc aaatggtgtg aacgatgaaa
     9361 agaacaaaac agcaccagta tctgcacatc cagatccagt agtccctaac gttcaacaag
     9421 gagtagaaga gaagaaacat gatgcagaaa agcatgcacc tcatcatcca ggaactccag
     9481 aatctaaagt tccagaacaa catgatgcaa tcgatccttc taaacctgtg gaagagcaaa
     9541 aacatccttc tgaccatgcg aaagcacaag aaaaacttag ttctgaccac acaaacggac
     9601 aaggaaaact tggttctgat catacaaata accaaggaaa gcatgattct gaacaggaag
     9661 attcaatgcg cggtgagctg aattatgaag ataaagttgt gcaaaaaatc atcggaatcg
     9721 caattgaaca agtagacggt ttattatctg caaacggtgg tttcttctct aatgtggcag
     9781 gaaaacttgt gaatacagat aatgtaacgg ctgggatcga aacagaagtc ggtaaaaaac
     9841 aagtagcagt tgatatggat gtcattgtgg aatatggcaa agatatcgaa aaaatctttg
     9901 aagaaatgca agaagtaatc ggcaaagaag taaaaaatat gacacattta gaagtgatcg
     9961 aagtcaacgc taacgtagtg gacatcaaaa caaaagaaga atttgaaaaa gatcaagaaa
    10021 cggtgcaaga caaagtaact gacgcagcga aaaaaaccgg tgaatttgct tcgaatcaaa
    10081 cagataaagt aaaatcagca gctggaagcg gcgcacaaaa agtaaaagaa aatacagaac
    10141 cacgggttca gtaataagga agtaaaacat taggaggaac gaaacatggg atttatttgg
    10201 tcacttatcg tcggaggaat tatcggagca attgcaggag caatcacgaa taaaggttct
    10261 tcgatgggga ttattgcaaa tgtcgtagca ggtttagttg gatcagcact tggtcaagca
    10321 ttattaggtt cttggggacc aagtattgca ggaatggcaa tcgtgccatc attgattggt
    10381 gccatcattt tagtactaat cgtatcattc tttgctcgaa aactgtaaaa agacttttaa
    10441 cagaattagg cagaagtcgc ccatcctcta taggtgggtg atgaatgccg ttcggtatga
    10501 gggttaccgt tggtaactcg aagaccgaca actaaggcgt attgcctt
//
LOCUS       DAHHZJ010000105        10343 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.106, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000105 DAHHZJ010000000
VERSION     DAHHZJ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.106"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            6..305
                     /locus_tag="NBL02_001999"
     CDS             6..305
                     /locus_tag="NBL02_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCD2572677.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="NBL02_002000"
     CDS             378..1739
                     /locus_tag="NBL02_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCD2572678.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="NBL02_002001"
     CDS             1761..2021
                     /locus_tag="NBL02_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572679.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="NBL02_002002"
     CDS             2035..2787
                     /locus_tag="NBL02_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HCD2572680.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="NBL02_002003"
     CDS             complement(2881..3486)
                     /locus_tag="NBL02_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD2572681.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="NBL02_002004"
     CDS             complement(3539..4513)
                     /locus_tag="NBL02_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HCD2572682.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="NBL02_002005"
     CDS             complement(4891..5610)
                     /locus_tag="NBL02_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCD2572683.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="NBL02_002006"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="NBL02_002006"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HCD2572684.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="NBL02_002007"
     CDS             7161..8558
                     /locus_tag="NBL02_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCD2572685.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="NBL02_002008"
     CDS             8639..9079
                     /locus_tag="NBL02_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCD2572686.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="NBL02_002009"
     CDS             9227..9553
                     /locus_tag="NBL02_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCD2572687.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="NBL02_002010"
     CDS             9554..10303
                     /locus_tag="NBL02_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572688.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
BASE COUNT         3277 a         1825 c         2144 g         3097 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgt
//
LOCUS       DAHHZJ010000106        10244 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.107, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000106 DAHHZJ010000000
VERSION     DAHHZJ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.107"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            371..640
                     /gene="rpsN"
                     /locus_tag="NBL02_002011"
     CDS             371..640
                     /gene="rpsN"
                     /locus_tag="NBL02_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCD2572689.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            655..834
                     /gene="rpmF"
                     /locus_tag="NBL02_002012"
     CDS             655..834
                     /gene="rpmF"
                     /locus_tag="NBL02_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCD2572690.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            866..1243
                     /locus_tag="NBL02_002013"
     CDS             866..1243
                     /locus_tag="NBL02_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572691.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1264..1413
                     /gene="rpmG"
                     /locus_tag="NBL02_002014"
     CDS             1264..1413
                     /gene="rpmG"
                     /locus_tag="NBL02_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCD2572692.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1437..2420
                     /locus_tag="NBL02_002015"
     CDS             1437..2420
                     /locus_tag="NBL02_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCD2572693.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2450..2572
                     /locus_tag="NBL02_002016"
     CDS             2450..2572
                     /locus_tag="NBL02_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HCD2572694.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2590..4113
                     /locus_tag="NBL02_002017"
     CDS             2590..4113
                     /locus_tag="NBL02_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HCD2572695.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4137..4376
                     /locus_tag="NBL02_002018"
     CDS             4137..4376
                     /locus_tag="NBL02_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD2572696.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4569..5030)
                     /locus_tag="NBL02_002019"
     CDS             complement(4569..5030)
                     /locus_tag="NBL02_002019"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCD2572697.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5225..5443)
                     /locus_tag="NBL02_002020"
     CDS             complement(5225..5443)
                     /locus_tag="NBL02_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD2572698.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5561..7177
                     /locus_tag="NBL02_002021"
     CDS             5561..7177
                     /locus_tag="NBL02_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HCD2572699.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7418..8758)
                     /locus_tag="NBL02_002022"
                     /pseudo
     CDS             complement(7418..8758)
                     /locus_tag="NBL02_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9034..9189)
                     /locus_tag="NBL02_002023"
     CDS             complement(9034..9189)
                     /locus_tag="NBL02_002023"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HCD2572700.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(9485..9817)
                     /locus_tag="NBL02_002024"
     CDS             complement(9485..9817)
                     /locus_tag="NBL02_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCD2572701.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
BASE COUNT         3416 a         1619 c         2020 g         3189 t
ORIGIN      
        1 aggaataatc aaagttttaa aaaattggcg ataaaccagt taaacataaa agattgaata
       61 aaaagttata gtttctttca tattgccgtt ttaagtagag gaaaagctac ttaaaacggt
      121 atttgtgttt gattggacta ataaaagtga attcttattt ttcagagcct aaactgcata
      181 ttcttttaaa tgatttgatt atctctatgt accgataaat tcgttttcaa atagttatct
      241 attcatatga gttttatttc atgttattta ttgttgacaa gagcaactct tgttggtaaa
      301 gtatcagtgt caaaacgtaa cgattacgat ttgaggtgtc taaatcaaaa taaaaggagt
      361 ataaaggtat atggcaaaaa agtcgaaaat tgctaaagca aagaaacaaa tggcgatgat
      421 tgaaaagtat gcagataaac gtcaggaatt aaaagccgca ggagatcgca ccgctctcgc
      481 taaattgcca agagattcaa atccgaatcg tctgaggctt cgtgatcaga cggatggtcg
      541 tccaagaggg tatatgcgaa agttcggtat gtcccgtatt aagtttagag aacttgccca
      601 tcaagggttg attcctggag taaaaaaagc aagttggtaa aaggaggaaa tatcatggca
      661 gtaccagcaa gaaaaacatc taaggcgaag aaacggttaa gaagaactca tcaaactgta
      721 gtaaaaccag aaatttcatt tgacgaagcg aatggtgatt atagacgtag ccatcatgta
      781 tctttaaaag ggtactataa tggaaagaaa gtaattaaag gagcttcaaa atagtttaat
      841 atataacttt taggagggaa tcaaaatgga aattatttac ccacctttag ttgaagaagg
      901 attaaagtat tatcttgaaa cgacccagca atcgctagat aaatgcactt tttatcgttc
      961 gatggtagag agaggaatta ttactgaaac gggtttaccg acccaacagg caattgaaaa
     1021 tggattagtc aaagattact atgaggacca aggcttatca tttgatgatt ttttaagaat
     1081 ctatccaatt tttgaagagt atgacgagga gttatttcaa tgtatcgatg gttattggga
     1141 gatacctgtt gatatgaaag aaaatttagt ttcacaattg gaatctggcg agttaacttt
     1201 tgaagatgca caacaaattc aagcatatct tgaagatcga taagtattga aggaggaaga
     1261 tttatgcgtc aaaatattat tttggaatgt gtcgaaactg gagaacgtct gtatctcacc
     1321 agcaaaaata aacggaataa ccctgaacgg ttagaattaa aaaagtattc gccaaaattg
     1381 cgcagacggg caatttttaa agaagtgaaa tagctaagtg gtaaaaaggt gacaaaatgg
     1441 gcataccagt tacaatcgtt tctgggtttt taggggctgg aaaaacaacg ctaatcaatc
     1501 aagccttgca agtctcaccc taccccaggg aagaaattat tataattgaa aatgagtttg
     1561 gtgaagttgg gattgatcac aaattattag ttcatagcga ggaacgggtg ttacaactaa
     1621 ataatggatg tatgtgttgc agtcttcgca gtgatttatt agcaatattg atttctttac
     1681 tggaaacaat agaagaacat caacaaccaa tttcacaaat catcattgaa actacaggta
     1741 tagcagatcc ccaaccaatc attcaaacac ttttaacagc gcctcaacta agaggaagag
     1801 tatatatcga tagtttattg actgttattg atagtgacag ttttgaactt attgaaagag
     1861 aaccacaagc ccttaagcaa cttgtcatgg cagatcgcat ttttgtgtct agaaaacaaa
     1921 acttgaattt atctgcacta aaaaaattac agagagaaat caaaagtatt aaccctttat
     1981 ccgatattcg aatgttcaca catagtgaaa cgattcaaaa aaaggatttt tataatttga
     2041 aaaaatttaa tcatccctta gtttttgaaa gtgattcatc agaaaatcac tataatggca
     2101 aatactatca tcaacaccat caagtacatg agtttaaatc aattctctta acttcagatc
     2161 aagacataaa aaaagatttt ttactccaat ggattgactg gctgatttta accaaccaag
     2221 ggagcctgta tcgttttaaa ggattgattg ctttacaaga gcaggatttt atagttgcca
     2281 tgcaagaggt aaacgaacag gttaattttc aatacaccac tcgacaaaaa gaccaaacca
     2341 ctattattct aatcggaaaa atgttggatg aagaaaggat caaacagagt tttgataaat
     2401 tactggcgga aagcaaataa tgataagtag tgattggggg taatttctaa tggagaaaac
     2461 ggatatatct agtgcatatc gacgacttaa aagcccgaat attaaaacta gaaaaagggc
     2521 attaaaaatt atcaaagaac ataaacgaaa taaaatgaag aaattagcat aattaaggaa
     2581 ggaaaaagaa tgaagaaaat cagtgtagga cttattggga tagcagctct aggattattg
     2641 ggagcttgct ccagcactaa tgatgcaaaa gtatcgaatg ataaagatgg aaaattagaa
     2701 attgtaacaa cattctatcc aatgtatgat ttcaccaaaa acatcgtcgg cgatgaagcg
     2761 aacgtggatt taatggttcc agcgggatca gaacctcatg actatgagcc ctcagctaag
     2821 gatatggcaa aagctcatga tgctgatgtt tttgtatatc ataacgaaaa catggaatca
     2881 tgggtaccaa aagctaaaga aagttggaag aaagctgggc ctaatgtagt agaaggaaca
     2941 aaagacatga ttttattgcc aggtagtgaa gaagaagatc atgatcacgg agaagaagat
     3001 caccatcatg aactagatcc tcatacgtgg gtttcaccca aaatggcaat taaggaagtt
     3061 tcaaatatca aggatcagtt agttaaattg tatccgaaaa aagcaaaagt atttgagaca
     3121 aatgctgaaa agtatttgac taaactgaaa cggttggatg ctgactatac cactagtctt
     3181 aaagaagcga aacaaaaaag ttttgtgacg caacacgcag cctttggata tttagctttg
     3241 gattatggat tgaaacaagt tcctattgct ggactaagtc ctgaagaaga gccatcttct
     3301 ggtcgattag ctgaattaaa agagtatgtt aaaaaaaata agatcaatta tatttacttt
     3361 gaaaagaatg ctaatgataa aattgcaaaa actttggcca acgaagctgg gataaaattg
     3421 gaagtactga atcctcttga aagtcttaca aaagaacaaa tggataatgg tgaagattat
     3481 gtttcagtaa tggaagacaa tctaaaagcg ttagaaaaaa caactatggt tgctggtgaa
     3541 gaggtagttc cagaaaaaga agcgaaggat gaaaagacag ttgctaatgg gtactttaaa
     3601 gatgccgatg tgaaggatcg cgaattgagt gattatacag gcgaatggca atctgtttat
     3661 ccgttgttaa aagatggaat tttggatgaa gttttcgatt acaaagcaaa aatcaataaa
     3721 gacatgacag ctgccgagta caaagattat tatactactg gctataagac agatattgat
     3781 actatcaaca ttaaagacaa caccattgat tttgtagtca acggtgaaca tcatcaatac
     3841 acatacaaat atgttggata caaaattttg aattatgaaa aaggaaaccg tggtgttcgt
     3901 tttaattttg aaactgatga tgcaggtgct ggtcgtttta aatatattca atttagtgat
     3961 catggaattg caccaagtaa ggcagagcat ttccatattt tctttggtgg agaaagtcaa
     4021 gaaaaactct ataatgaatt gcataattgg cctactttct atccagcttc attgtctaaa
     4081 catgaaattg ctcaagaaat gatggctcat taaaattcaa tttttggagg aaatacatgg
     4141 taagaaaagt attattgtct atggattcca agaatattga tgcttatcgg cgttatcgat
     4201 ttgcaaagca aatgagatta aatgggagta cattagctga tattgctgca actttacatg
     4261 ttagtattga cacagttcat agagtattaa aacgggatcc tgattgttgt tttatcaagg
     4321 tcaatactgg aagaagtaga cgtttagcaa aacggcataa tagcttggcc aattaattga
     4381 taataccaga gtagatcatt tttcccttat ttggatttga tctgcttttt attatgtctt
     4441 gaattgtcaa atcaagcgca acactttatc aaagaagact tcataaggtg ttctccaatc
     4501 taaacacttt ctaggtctgt tattcagttt ttcaatcatc tgatttatcg ttgagttatc
     4561 taccgtagct atctctttcg attttggcaa gtattctcga atgagtctat tcgtatttta
     4621 atttgttcat tgttgccaag ggcatgagga tctggaaaat aaaagggcat attattcaat
     4681 gcttctgtaa ctgcgaaatg attggagaat tcttttcctc tatctggagt gatcgttttg
     4741 accgcctcat ttggaatact gcttaaaagt tcaatcattt tctcgctcac ataagcggct
     4801 ttctttctgg ggaccttctt tcctgctaaa aggaagcatg attttctgtc tgtcaaagtc
     4861 actaaacaag taccaccagt tttgccagct acaatatcgg ctttccaatg accaaaatca
     4921 cttctatcat ctgcggattt tggacggcca tggatcgaat gagtaaggca aatctttcca
     4981 cgagactcga catgattttt tgtatgtctt gtttttcctc gatggtgcaa tgaacggatc
     5041 aggcctttat tgccgtgtga gaggggttct gtatcgaaaa gatcattgca aattgcacgt
     5101 tagatcgtga taaaactaat tgaaaagtcc atatgttcat gcttcaaacg attcgagatt
     5161 tcctctggcg accactgaaa atcgacaaat aaatgacgaa caattgacca aactcacgga
     5221 tcatttagaa tgtgataacg tccgcatttt tttcttcgat aagcataatt tttttccgct
     5281 ttggaaggtg aatagttttt ttcttgtttc ttctcagttc atgtaaaata gtaaacgggc
     5341 tatgctgaat gtcgttgcca attatactaa tagaaaatcc ttcttcgtga agcagaaaga
     5401 ttctttctcg ttcgagtata gtaagatgac gatactggct cataattatt ttccttctag
     5461 gagtgttctc gacaaactta tactagttgg atatcgatta tgactcattt ttatgtgttg
     5521 cacttaaagt gtaaattcaa ggtatagaaa gaggttccct atgaaaaaaa tacctattac
     5581 aatcttaact ggctatcttg gttcaggaaa aacgatactt ctaaatcata ttctgtacgg
     5641 tgatcacgga aaggaaattg ctgtcattgt aaatgacata ggggaagtaa atgttgatgg
     5701 ggtattagtc gacaagaaag gttttaaaag aactggagaa aaacttgttt cgatgccgaa
     5761 tggatgcatt tgttgtacat tacgcgaaga tttgattgtt gaactagatc aattagtgca
     5821 attttctgac ttgaatcaaa ttgttattga agcttcagga atcagcaaac caattccaat
     5881 tattcaagca attatattag ctaaaaaccc tttagacatt gatttgaccc aacgattaga
     5941 gattggtgcc attattatag tagtggatgt aaaacgtttt tgggataatt tcgaaacggg
     6001 tgaaaagatt caggaaagaa aagctgaagg taatgtagtt gaagaagaac aggatattcg
     6061 tggcttgtta attgatcaat tcgagtgttg taacatatta cttttaaata atagtgactt
     6121 agtgtcaacc aagacgattt acaaaattaa cggtctaatt gctaagttac agtccaatgc
     6181 caaaataatt aggacaatta aaggtgaagt agacttatcg aaaattctaa atacaaatct
     6241 ttttaatata gatgagactt cctattcagt gggctggttc aaagagttag agcttggcta
     6301 tcaaaatcat attcctgaaa cagaagaata tggtatctca agttttatct atcgaaatcg
     6361 gttactcctt gacgcacaaa aatttgccca tcttacggat aaaaattttc ctggcaatat
     6421 tacaagagca aaagggtttc tttggtttgc ccaacatagt gatttttcga cgatctttga
     6481 aacatcgggt agctataaaa gtattgaatc attaaattat tgggtagcta gtttacctaa
     6541 aattgagtgt caaatgatta tacaaaaaaa tcccgaattg aaggaagcct ggcatccgtt
     6601 gtttggtgaa cgcattaata aactagtttt tattggtatg aagatggatc aacaatgtat
     6661 taaggaacaa cttgagggtt gtttaactga ttctcttaat gcagataatt tccgaaaaat
     6721 ttcaccattt ccatggaaaa agtctaatat gtttagccag atatcgttaa tgaaattttt
     6781 aagaaaagct ggtgtcaaga taggcgatgt cataattatt ggcattttga ttattacctc
     6841 ttttattccc ttatttgtat tctctataga aaaggagaat gaaacaactg aagatgctat
     6901 caaatatgcc atcgttcgaa ttgatggcaa ggaaatggat cgcttcgatt tggataagat
     6961 caatcataaa ctcgtgaatt atcatcctgc gaagggacaa tataatattg ttgagattaa
     7021 agatggaaga attcgagtta aagaagataa tagtcctgat caaatcgcgg ttagaactgg
     7081 atggatctca aaacccggtc aaacgagtat ttgtttaccc cataagttag ttattagtat
     7141 tgagaaaaaa gaaagcacag attactacat ttactaatct ggaaatagat gaggaggttt
     7201 ttaataagaa aaagatatta ttattaataa tagcaggggc tttattaagt gtgttaagcg
     7261 cttgtggaca agaaaaagaa gcggtgacaa caggcacaag tatctttaac gggacctgca
     7321 atggtttagt taacagtcgg ttagattact tattttggct cttcgtcaat gagtgttggt
     7381 gaagaaattc aatagaataa tcgagatact cacacggcta gacagctatc ttgtctagcc
     7441 gtgttgttgt tctggcttta gtcagttggg tagtattctt agttttctta aaatattttc
     7501 ctcgatataa aaagattcgt tctcggtagt tttgaaagtt tctatagcca taggcaattc
     7561 gttttagtac tttgatatgg ttattcaaac attctatagg gccgttggaa tagggaacgc
     7621 gcaacgctcg tttgatttct gtttggtatt tcttgaaggt cgtgaacact ggttggtagt
     7681 gctctggcaa ctccttgtag tcttctttta ggagcgcgac gaattcagga acatcttgtc
     7741 ttctgataac ggaaagaaaa tcctggtaga cttcgtagcc tcgctttaag gaatcatcat
     7801 aagataatag ccgatcgact atctctgctt ctgtcagatg atctcgaaat gacggacgcc
     7861 agtagcgatg ctgacctttc aattcccacg cattcttttg aagtagtttc cagtatttct
     7921 ttaagtgctt cgaccgacga tccccttttt taaaggtctt cattacttga attcggtggt
     7981 tggtaaaagc ccgaccaatg tgttggctaa tatgaaagcg atcggcgacg attcgggcat
     8041 tcggaaaaca ctctttcacc aatgtgcgat agggtgtata gaaatctgaa accactagac
     8101 gcacttgttc acggactttt atagggtagc gcaagaaata gtcgcgtaaa ggattgagtt
     8161 gccgattttc cacaatatca atcagttgat gggtcttgcc gtccatcatg acaaagctca
     8221 tggctcccga gacatttttc actgatttga attcatcgaa acacaccact tctggtaaca
     8281 gctggctcaa agaaatggtt ttaggccgaa ggctgcgaag tattcgaatg acagtggtcg
     8341 gagaaatgtg cttcatacgt gcgatcaaag acatagaaag aggttcgctt aatagctcaa
     8401 gaatggcttg tttgactctg cgagcaatgg agcagcgacg atcactaact gaatcttctg
     8461 ctaaaaaagt ctttccacag tgtttacatt tgaagcgctg tttgttaatc cgcagaatgg
     8521 tcttgtagtt actgacatca ttcaataaga tggatacttt tttccagccc tatttaatga
     8581 tcgtttgttc attttgatag tggcgatgaa agcattcctc cggttgatag gtcaatgtgc
     8641 catcaaaaac aaaacatgtt tcgtcattga ttcgctcttt gcgaaaggca tcttcatgaa
     8701 agataagatt taggtctaga atatctagta actctttggt aaactctgaa taggacatct
     8761 tgaaacactc ctttgaatgg ttttcgtcga ctttattcta aaggatttta aggtgtctgt
     8821 ttgcgtttat tagaaaaaag aaaaagtatt ggcaacagaa ggcgtcacca acactagata
     8881 ttatagatcc cttattttct tcgcttcaaa ttgtcaagga gtcatcataa attttgtatg
     8941 gattcttttg tttgggtagt agtcttctaa ataatcttgg attaacacct tcatctctat
     9001 tttactttta tatttgttgg agtcgattaa ttctcatttg attgatcgat gaaacgcttc
     9061 aattactgca ttgtcaacag gagtcccagt ttggctcact gaatgtgtga tgtggtggtc
     9121 gttcaacagc tcttgataaa taaaagaccg atgctggcta cctctatcgg aatgaattac
     9181 cgtaggcaat tctccttaat aaatttgtaa tggacggacg acaatctctt ttttcatgtg
     9241 tgaatctacc atatagtaac gtagaatacg agttgctaag tcaatatagg tggacaaata
     9301 gaggcgaccg tcattacatg gaatgtaagt aatatccatc acccaaacag catcaatttg
     9361 gttctgttca aattatcgat tcaacatttt tttaccattt ctttgattgt tttttctcta
     9421 ccatgagaag atttgcgtcg atgaaatcct cttgtgcata aattcatttc acgcataaga
     9481 acagctacaa cacgtttatt gatttcgatt ccttcatcgt aaagtttttg agtgatccct
     9541 ggagatccgt atcgatcttt gtgttcgtag aacactcgtc ttcctctttt agtcgtttat
     9601 tttcctgttc aagaatgtag aaatctgctt tatcaggtaa tctttttcct tgtccagcaa
     9661 aagcattttt cccgtatcga cgatattctg aaacccatcg ggaaattgtt ggacgagaga
     9721 tattgtattc ttcagcaatt ttcttggtag ttactttttt ctcaaggata tcctgtgaaa
     9781 tctttagttt aaatttacga tcatatgttt ttctcaatag agttcattcc ttccggtaac
     9841 gtaactgata gtcaagaaaa gtgtatcata gcccaactta gtatcagggt ttgtcaacta
     9901 aactgtggaa gttgatagat aagagttttt aacaaccaaa attctcttga ctatttttga
     9961 aatcagacaa attttgattg gacacatagt tgaataaatc taccgtcgtt acggaaggta
    10021 aatcgcatgc ttttcaattc tagaggagaa gtcctcgttg aagtaccata gcccagttct
    10081 ggattcgacc tcctgcccac ttcatacgta aataaagtaa ttttaggagt gagactgtga
    10141 caaaagttat ttttaagcta aaaacgaatt aaaaaacgtt gataattaaa atcattcgtt
    10201 ttagattcta tgaatcatta tagaacgctg taaaatcgat actt
//
LOCUS       DAHHZJ010000107        10014 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.108, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000107 DAHHZJ010000000
VERSION     DAHHZJ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10014
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.108"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(19..339)
                     /locus_tag="NBL02_002025"
     CDS             complement(19..339)
                     /locus_tag="NBL02_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HCD2572702.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(487..2721)
                     /locus_tag="NBL02_002026"
     CDS             complement(487..2721)
                     /locus_tag="NBL02_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCD2572703.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            3200..3466
                     /locus_tag="NBL02_002027"
     CDS             3200..3466
                     /locus_tag="NBL02_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HCD2572704.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            3466..5193
                     /gene="ptsP"
                     /locus_tag="NBL02_002028"
     CDS             3466..5193
                     /gene="ptsP"
                     /locus_tag="NBL02_002028"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCD2572705.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            5357..6403
                     /locus_tag="NBL02_002029"
     CDS             5357..6403
                     /locus_tag="NBL02_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCD2572706.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            6420..7643
                     /locus_tag="NBL02_002030"
     CDS             6420..7643
                     /locus_tag="NBL02_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCD2572707.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            7732..8619
                     /locus_tag="NBL02_002031"
     CDS             7732..8619
                     /locus_tag="NBL02_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCD2572708.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            8705..8935
                     /locus_tag="NBL02_002032"
     CDS             8705..8935
                     /locus_tag="NBL02_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HCD2572709.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(8992..9957)
                     /locus_tag="NBL02_002033"
     CDS             complement(8992..9957)
                     /locus_tag="NBL02_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCD2572710.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         3190 a         1772 c         2028 g         3024 t
ORIGIN      
        1 attatgttca aaatttgttt acacggctaa tgtagcgcct aattcatgtt ttggaatcga
       61 gatttcaaca aggcctgcca attcgattac tttgatttca tcctcgtggt aaacggcgtc
      121 tttgatttta tctaagaaat aatttttagc atcttcgatt tctttcggct ggattttacg
      181 tttgtcattg atcagcagaa tttcattatg ttcaggggct tcagtgtaga caatagtggt
      241 atttccggct gtgtagacct tgactagatg ggcatcagtg ttttcaagct ggtttaatac
      301 aagacgggaa tggctgtttg ttacatttac caatttcatg gcgctcacct cttttatgta
      361 tttttaattg cttgtttctc ttattataat ttctttttta taaaatgaac agtattttga
      421 cttttctgaa aaaagaatga gacggcattt ctcaaagaaa taccgtctca cgctataaaa
      481 ctggatttat tcagccgttt cttcggctga ttctttagcc aaacgttctg gtttagaagt
      541 aacgatgatt tttccatctt tatccagttt tgctttcaac tcatggatag aagggtgatc
      601 aagataaaat tcagcgatac catcttcgat ttgttcttgg atcgtgcgtc gaagaggacg
      661 agcacccata gatggatcat agcctaagtc aactaatttt tctttgacat tcgtaggaac
      721 ttcgatgtga agttgctgtg ctgccagcat ttggttgaca tcgtctagca ttagtgtgac
      781 gattgtcatc aagttttctt ttgataatgc actgaattcg atgatcccgt caaaacggtt
      841 cagaaattct ggtgtaaagt agttgtttaa ttggttcaat acagaacgag taacaccttc
      901 gcgtgccgca ccaaaaccaa cgtttgcttc tactttacca gttccggcat tacttgtcat
      961 aatgatgatc gtatctttaa agctgaccgt tcttccttgt gcatctgtca atcgaccatc
     1021 atctaagatt tgcaagaaca tatgcaaaac atctggatga gctttttcta cttcatctaa
     1081 caagacaaga ctgtaaggat ttctccgtac tttttctgtt aattgaccag cttcttcgta
     1141 tccgacataa cctggaggag aaccaatcaa tttagagaca ctatgttttt ccatatattc
     1201 agacatatcg aaacgaatca ttgaatcttg tgaaccgaat agttcgtagg caagttgttt
     1261 cgcaagctct gttttaccta caccagttgg tccaacaaat aagaaggagc cgattggacg
     1321 attctgtttg tttaatccaa cacggtttcg gcgaatcgct ttcgctactc gatctacagc
     1381 gttatcttgt ccgatgacat gagctttcaa gtcatctgct aagtttttca gttgtgtctg
     1441 ttctttttct tttaattcac ccactggaat acctgtccgt tgttcaacga ttttttccat
     1501 atctttttcg gtaatcacag gtgtttcctc ttcagtgagc tgacgttctt tcattttttg
     1561 aagtttattg atctgatcgc gataataagc tgctttttcg aaatcttctt ctcgagaagc
     1621 taaaactttt tgttcttctg cttcttgcag ttttttctcg attgttttag gatcaacgat
     1681 ttggatcgtc aagtttttct tagaacctgt ttcatctagc aaatcgatgg ctttatccgg
     1741 caagaagcga tcttggacgt aacgattgga taaagttgcg gctgcttcga tggcctcatc
     1801 agtatatttt acatgatgat agtcttcata acgtttttgc aaacctttca aaatactgat
     1861 cgtttcttcg acagttggtt catctacccg tacaggttgc atacgacgtt ctaaagcagc
     1921 gtctttttcg atgatgcggt actcatttaa ggtagtagca ccaaccattt gcaattctcc
     1981 tcgagctaaa gctggtttta aaatattacc agcatccata tttccatcac cagcagaacc
     2041 agcaccgacg atttcgtgaa cttcatctat gaataggatc acattttctg cttgacggat
     2101 ctcttcaatc aatttttgca tacgttcttc aaattgtcct cgtataccag taccttggac
     2161 taaagatacg acatctaaac gaatgacttc tttgtccatt aatttttgcg gtacatcacc
     2221 atctacgatt ttttgtgcta aaccttcaac aactgctgtt ttaccaacac ctggttctcc
     2281 aatcaagaca ggattgttct tggttcggcg atttaaaatt tcaatgactc gtttgatttc
     2341 gtcatcgcgt ccgatcacag gatcgatatc accttgtcgt gcttgttctg tgatattgat
     2401 tccgtattct cccaataaac cttctgtatt gccttgaggt ggctgaccac cattgaagtt
     2461 gttgtttccg ccaaattgtg ttggtggtgt ttgctcataa tttccttgct gttgcatttg
     2521 gcgagacatg gaacggaaaa gatcgtccaa actgccaaat ccaaatggat cattttgggc
     2581 catattagtc agacctccat tttgttgatt tttgattttt tgataacagc tctgacagta
     2641 atcgagttgt gtccgctgac cattgacagt ggcatacaag tgaatcgttg cctcattctt
     2701 gccgcagttt tgacaaagca tgaatcattc acgtcctttc acaaaaatgg gtacatccat
     2761 attttacatc tcgtttttca tgaggtaaaa gaataacccc tagtttttta agagatttat
     2821 gaggtagaaa aggtgaaatc tctattgacc attcctgacc tttgactctt attatactaa
     2881 aaataaaaag ggaagcaagt aattcgtttc atatgaaaac aaagagggtc aaaggaaaga
     2941 atgataaacg tcaggagaag agaaagcgac aagaaaaagg aaataaacac gtttgttata
     3001 aaatgtgaag aaaaggatga aaaagaatcc ggaataattt gaatactttt atctgaaatt
     3061 gaatgaaaac tgttgtatct aacgaaaaaa aatgttaatc tttacagatg aaagggtggc
     3121 atttgttagg attctctgaa ttactaaaaa agaaaaccct cacataaaaa caacactcac
     3181 ttaaaggaga ccgattaata tggaaaagaa agaatttcac gtagtagcag aaactgggat
     3241 ccacgctcgt ccagctacac tattagtaca aacagcaagc aaatttaact ctgatgtaaa
     3301 cttagagtat aaaggtaaat cagtaaactt gaaatcaatc atgggcgtta tgtctttagg
     3361 tgtaggtcaa ggttctgatg ttactattac tgcagaaggt gctgacgaag ctgatgcaat
     3421 ggcagcaatc gttgaaacaa tgaagaaaga aggcttatct gaataatggt tgaaatgcta
     3481 aaagggattg ccgctagtga cggtgtagcc gttgcaaaag cttacctgct agttcaaccg
     3541 gatttaacat tcagtaaggc aacagtagaa gatactgcgg ctgaagaagc tcgtttggat
     3601 gctgcattag ctaaatctac tgaagaattg cagcaaattc gtgaaaaagc agcgcaaagc
     3661 ttaggtgaag cagaagcgca agtatttgat gcccatttga tggttctttc agatccagaa
     3721 atggtaggtc aaatcaagca aaatattaaa gacaacagcg taaatgctga atcagcgcta
     3781 aaagaagtaa cggatatgta tatcggtatg ttcgaagcaa tggaagataa tgcttatatg
     3841 caagagcgtg ctgcagatat tcgtgacgtt gctaaacgta ttttagcgca cttattaggc
     3901 gttactttac caaatccatc aatgatcaac gaagaagtgg tcgttgttgc acatgacttg
     3961 actccaagtg atacagcaca attagatcgc aattttgtaa aagcgtttgt aacagacatt
     4021 ggtggacgta cttcacattc tgctatcatg gcacgttctt tagaaattcc agcaatcgtt
     4081 gggacaaaag aaattactgc taaagtcaaa gaaggcgata ttttggctgt caacgggatc
     4141 gaaggcgatg taatcatcga tcctacagat gaacaaaaag ctgaatttga aaaagcaggt
     4201 gcagattacg cagcacaaaa agcagaatgg gaaaaattaa aaaatgcaga aactgtcaca
     4261 gctgacggca agcattttga attagctgca aacatcggaa caccaaaaga tctagttggt
     4321 gtccataata atggtggaga agcagttggt ctgtaccgta ctgaattcct gtatatggat
     4381 tctccagact tcccaacaga agatgaccaa tatgaagctt acaaagctgt attagaaggt
     4441 atggaaggaa aacctgtagt tgttcgtaca atggacatcg gtggagataa agaactacca
     4501 tatctgcaat tacctcatga aatgaatcca ttcttaggat atcgtgcatt gcgtatcagt
     4561 ttatctgaac aaggcgacga aatgttccgt acacaaatgc gtgcattgct tcgtgcatct
     4621 gttcatggta atttacgtat catgttccct atggtagcta cactcaaaga attccgcgca
     4681 gcaaaagcga tctttgaaga agaaaaacaa aaattgataa gtgaaggcaa ggaagtatct
     4741 gatacgatcc aagtcggcat catgatcgag attccagcag cagcagtctt agcagacaag
     4801 tttgcaaaag aagtagattt cttctctgta ggtacgaacg atttgattca atacacaatg
     4861 gcagcagacc gtatgaacga acgcgtttct tacttgtacc aaccatataa cccatcaatt
     4921 ttacgtttga tcaaaaatgt aattgatgca gcacacgctg aaggaaaatg ggcaggtatg
     4981 tgtggagaaa tggctggaga tcaaacagct gttccattgc tagtaggtat gggtctagat
     5041 gagttctcaa tgagtgcgac atctattctt aaaacgcgta gtttaatgaa acgcttggac
     5101 acagcgaaaa tggctgaact tgctgatcgt gcattgaaag aatgcgatat gatggaagaa
     5161 gtcgttgaac ttgttcatga atatgtaaaa taaacaacgt atcaaaagct ggtgtggctt
     5221 gtaaaagagc tacgccagtt tttttgttct tttttaagaa aacgtttttg tcagtaagaa
     5281 tagacaaaaa tagggcttaa gtttgagtga cagtttgtag gctatgttat actatataag
     5341 tgaaggggga cgtaaggtga aggtattatt atattttgaa agtgaaaaga tgcttgcaaa
     5401 atctggaatc gggcgtgcgc ttgatcatca aaaacgtgct ctgacggaag tcggtatctc
     5461 ttatacacta gatgaaaaag aagattatga catcctgcac atcaatacat acggaatcaa
     5521 tagtcataat atggtcaata aagccagaag agaaggaaaa aaagttgtct atcatgcgca
     5581 ttctacagag gaagatttcc gaaattcctt tatcggatcg aaccagcttt ctcctatcgt
     5641 aaaaaaatat ctggtaggat tgtaccaaaa agcggactat ctgatcacgc caacacctta
     5701 ttcaaaacag ttgttggaaa gctatggtat ccgtgtgccg attcaagcta tttccaatgg
     5761 gatcgactta gagaaatatc accctgatcc aatgaaagaa caaaagttca gagaatattt
     5821 caaactgtct ccggatcaaa aagtaatcat ttgtgtcggt ttgtttttcg aacgtaaagg
     5881 aatcattgat ttcgtagaaa ttgctaagaa gatgcccgaa tacacattta tttggtttgg
     5941 tcatgttccc atgtactcga ttcctcggaa catccggaaa attgtaaaag aagatcatcc
     6001 tgaaaatgtc ctgtttcctg gttacattcg tggagagatc atagaaggtg cgtattcagg
     6061 tgcagatcta ttctttttcc cttcttatga agagacagaa gggatcgttg ttttagaagc
     6121 tttagcaagc aaacaaaacg tacttgttcg agatattcca gtgtataatg gctggttaga
     6181 agatagcaag aactgctata tggggaagac aaacgaagaa tttatccgac tgatccaaca
     6241 gattaccaat caagaactac cgagtacgac acaagccggt tatcaaacag caaaagagcg
     6301 aagtatcaat aaaatcggtg aagaacttaa aaatgtatac gaaagtgtac tgaatgaaaa
     6361 agtctctagc aaattaaaaa aagtaaatca ggtcaaggac tagtttggag gcgaaaaaca
     6421 tgaaaatcgg cttttttacc gatacctact ttccgcaagt cagcggagtt gctacatcga
     6481 tcaaaacatt aaaacaagaa ttagaaagac atgggcatga agtctatatt tttacaacga
     6541 ccgatcctaa tgcggataag ttggaaaaag atgtgattcg aatgccaagt gtaccattta
     6601 tttctttcaa ggatcgaaga gttgttgtta gaggcatgtg gtatgcttat ttggttgcca
     6661 aagaactaga actagatctt attcataccc atacagaatt cggagcaggg atacttggga
     6721 agatggtagc taagaagttg aaaatacctc tgatccatac ttaccatacg atgtatgaag
     6781 actaccttca ttatatcggt aaaggaaaag tgatcaggca atcccatgta aaatacttgt
     6841 ctagattatt tgcaaatcat acgacaggtg tagtttgtcc aagtgaacgt gttattgata
     6901 cattacgcag ctatggtgta atagcaccgc ttcgcgtgat tcctactggt atcgacattg
     6961 aaaaattcaa acgttctgat atcactcagc aggatatcag caaattaagg gagtcactag
     7021 gtcttcagga aaaccatttg atgattcttt ctttaagcag gatttcttat gagaaaaata
     7081 tccaagcctt gattaatggg tttcctcagg tcatctccgc ttatcctaac gccagattag
     7141 ttatcgtagg aaaaggacct tatgtagaag agttagagga attgatctca gaactgcagt
     7201 tagaagaatt tgtgcagttc acaggagaag tggacaacaa tgatgtagca ctttattata
     7261 aagcggcaga ttattttgtc agtgcttcta cttcagaaac acaagggctt acttatacag
     7321 aagcgatggc agccggaaca ccttgtgtcg tggaaggaaa tgcttatttg aaccgattgt
     7381 tcgatcatcc ttcattaggg aaaacttttg aaaccgacga agactttgca ccagcactta
     7441 ttgattatat ttccagtgaa gttcctaaag atccaaaagt tttagaagct aaactttatg
     7501 atatttcatc tacacatttc ggcgaagcta tgatcgaatt ttatcaagac atggttgttt
     7561 atcatgaaga agagatgcga agaaaagaag aagaagactc gattgaacga atcaaaataa
     7621 aattaaattc atttaaaaga taaaattcaa acatttttta tgaaaaaatc cttgtttcta
     7681 gcgtatacta gcatagaagc aaggattttt gatttttagg aggtcgaaaa aatgaaactt
     7741 ggttttatcg gaacaggcgt catgggaagt gctgtcgcac gccatctact agaagcagga
     7801 cacgaagtag ctgtctataa tcggacaaaa gcaaaagctg atcctttagt aacagaagga
     7861 gctatctggg cagatacacc aaaagcggta gcagaacaaa gtaatattct ctttacaatg
     7921 gtaggttacc ccaaagatgt agaagaaatc tactatggac agtcggggat tttttctgca
     7981 gatatctcag gacatatttt agttgatttg actacaagta caccaagcct ggcagaaaaa
     8041 attgcaaaaa cagcaaaaga aaaaggcgca gatgcgttag atgcaccggt atccggtggt
     8101 gacttaggag caaagaatgg tacactgacg atcatggtcg gtggagaaga agccgtttat
     8161 gatcaagtac tgcctttatt caaagaattt ggtacgacat ttacattgca tggttctgct
     8221 gggaaaggtc agcatacaaa aatggctaat cagatcatga ttgcaggtac gatgacagga
     8281 atgacggaaa tgcttgtcta tgcacaaaaa aacggacttg atttaaagaa agtgattgag
     8341 acgctttcag gtggaagtgc agctaactgg tctttaagca attattctcc tcgtatttta
     8401 aaagaagatt acacacctgg tttttttgtc aaacatttta tcaaagattt aaagattgca
     8461 ttagaagaag cagaaaaaat ggatcttgta ttgccagcaa ctacccaagc attgaaacta
     8521 tatgaagagt tggcagataa aggttttgag aatgacggaa cccaagcttt gatcaaactt
     8581 tggtggcctg aaggaaaaat accagaaaaa aaatcataaa aaacgagttt ttttagagaa
     8641 aatcaatgca agtaggctat gcttttgata caatataaca gaaataagaa aaggaggacc
     8701 tccgatgaca cattcacaat tagtcgcaat cataaaacga ctggaggcta tggtcgagtc
     8761 agcagataat gaactgcaag ttcgccgttt tgaaaaagaa ggcgtagaga agtgtacagt
     8821 aacttatgat aaagcgaccg atacatttga attaactgaa acagatactc atcagcaata
     8881 cgaatttgat aatatcgata tcgtagcgat ggaaatctat gacttaatcc aataaatttt
     8941 aggataattg acatttgaag ttgaagatgc ctaaaagaag ccgagaagca actaatttag
     9001 ttgcttttca gcttcttttt tgtttaacag ttcaatttca aactgttctt tttctaattt
     9061 tttgcggtaa ctgatcaaga tttgtacagg atcgatttct tcttttgaca attgacgttt
     9121 caaagataaa tgagcttctt tcttttcata atagaagtca ggagaaacgt caccagaggt
     9181 aatcattgtt tctgcaagtt gaccataaca aacgaggtag tgctttttct gactctcatc
     9241 tgctgctaat ggcatgctga aggtgagcga tggtgttctt aaaaactgct tttgttcctg
     9301 gatccgtcta ataaaataag taactaattc gatatctttt tgaagcatac cggctttttc
     9361 gaaattcaac tgatagatga aatgttgttg tctttttttc aagtaagtca gtagcttttt
     9421 atttcggcct tgaaacagcc cttggagttc cttttttttc tgttcaaaag tcatttcctg
     9481 taggtcagga agctggactc gcaaagctaa caaactaatt tcagacaacc aaggcatttg
     9541 ataagtttct tccattatac gcaaaataga cggcattttt ttatagagac gaaaaggccc
     9601 gtatgttcga gccgtaggag tatctgttaa tacaaatccc ttattggtaa tatgtacata
     9661 attataatta ctgaaataat tcatttgtcg gttataaaaa ggacggtaat gttggatcaa
     9721 ttgacactcg agcaataaag catctaattc cgtgtctact acgacgaaat caaaatctgt
     9781 tatatgatga atcatacgta atactttttt tgaatgctgt ttattctgat gaaaatagct
     9841 gcttactcta tttttgagcc gttttgcttt accaacatag atgaccttac catctcggtc
     9901 tttcataaga tagacacccg gcagcatggg gagagttttc attttttctt ttaacatagg
     9961 atctcctttt acgctttgaa taaaaagact ataacaaatt ttgaaataat ggac
//
LOCUS       DAHHZJ010000108         9943 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.109, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000108 DAHHZJ010000000
VERSION     DAHHZJ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.109"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(110..565)
                     /locus_tag="NBL02_002034"
     CDS             complement(110..565)
                     /locus_tag="NBL02_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572711.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(719..1927)
                     /locus_tag="NBL02_002035"
     CDS             complement(719..1927)
                     /locus_tag="NBL02_002035"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HCD2572712.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2039..2371)
                     /locus_tag="NBL02_002036"
     CDS             complement(2039..2371)
                     /locus_tag="NBL02_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCD2572713.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2519..3394
                     /locus_tag="NBL02_002037"
     CDS             2519..3394
                     /locus_tag="NBL02_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCD2572714.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3476..4057)
                     /locus_tag="NBL02_002038"
     CDS             complement(3476..4057)
                     /locus_tag="NBL02_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HCD2572715.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4063..5322)
                     /locus_tag="NBL02_002039"
     CDS             complement(4063..5322)
                     /locus_tag="NBL02_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCD2572716.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5324..6370)
                     /locus_tag="NBL02_002040"
     CDS             complement(5324..6370)
                     /locus_tag="NBL02_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572717.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6605..6880)
                     /locus_tag="NBL02_002041"
     CDS             complement(6605..6880)
                     /locus_tag="NBL02_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HCD2572718.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7123..8433)
                     /gene="der"
                     /locus_tag="NBL02_002042"
     CDS             complement(7123..8433)
                     /gene="der"
                     /locus_tag="NBL02_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCD2572719.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8615..9844)
                     /gene="rpsA"
                     /locus_tag="NBL02_002043"
     CDS             complement(8615..9844)
                     /gene="rpsA"
                     /locus_tag="NBL02_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCD2572720.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
BASE COUNT         2771 a         2114 c         1669 g         3389 t
ORIGIN      
        1 aaaatcttgt cacagtccct tattctgaat aaacggtgtg gaagagacag cctatcagta
       61 gtaataagac acatctcttt cacagcctca tttttgcgta aaaatcgatt tattcgaaaa
      121 attccccgta tacatcttct acacgttgat ctggattgat cacgatatac aagttacctg
      181 ttttttcgct gatttttgtt gattgatatt gattgtctaa tccttctgtt cctttatcta
      241 gatatgggaa gtacacaaga tcaggtgtgc catttcggta aaggatttcc attcgctcga
      301 tatcttcgta tttcaatgcg cgattaaaca tgcccatttc cagtccgcct aggttgatat
      361 catgtgtcgc aactgcgtcc ccttcatgga agatttcgat tttaaatcca gcacacggat
      421 ggatttcaac aaattcactg ccattgattc gtccaaaact tgttgtgatt tgtttgatcc
      481 aaagatcgcc gatatgacgg cggttgatcg tccaggtttc tccatttcta aaataaaatt
      541 ttagcgctgt catttcttta ctcattaaaa agactccttt atgatgtaat ttattcttgt
      601 actacctcag tttagcacaa atttatgtaa gtggtcacat cttatgtttt ttcttctaat
      661 gacgtatctg cccactttaa gtagccaaca aagctgagca gatacagtct agtgtttttt
      721 aaatcaattt ttctttcgat aaaaaatcca aaacctgttt tttatcattt tttattcttt
      781 gttctacgac ggctttttca gctaattgca aagcttcact aacccactta ccaggttttt
      841 ttccagtttc ttctagcaag attttaccat ctactgctaa ttcgcttaat gacttgatag
      901 gtaaacgatc ataggattct cctacggcat ccatctgagg tgtttcgtca aaatagatca
      961 tcaattcttc tacttgcagt gcatgttttt tgcctaagaa gtacaattcg aatggttcca
     1021 aagctccagt ttctcgttgt cgtagtcctt ggacaagtgc ttgaacgtct ttaatcattt
     1081 ggttagaaca cttccacgat ttcaaaaagg aacgaatctc attttctttt agatccagtt
     1141 ggctgatcaa taatgcccat gcttggctct cttctttcaa tgggacggat ggtaattcag
     1201 caaaacgtaa aagtgcttcg ccttcttctt tcaaaccggg acagcaaatg tagcactctg
     1261 tttcaataaa tgcatggatt cccgcggccc gttcatttcc caagagcatc ttgatgaatt
     1321 ccacatttat acgttctaca gatattttac ctaatagctc gtggttttct ttgatggcgt
     1381 agaaggtttc ttcctctaat ttgaacccta gttgactgac aaaacgcaac ccgcgcatca
     1441 ttcgtaaggc atcttcatgg aaccgctcat gaggattgcc tacagctcgc aagatctgtt
     1501 cttctagatc ttgcagacca tcgaacaaat cgatgatttc tccatcttct ttcaaagcaa
     1561 acgcattgat cgtaaaatct cttcttttca aatcttctgc caacgaacga acgaattcga
     1621 catgatctgg tctacggtag tcctgataag ttgattccgt ccgaaatgtg gtgatctcat
     1681 attgttcatc attttctaat accaacacag ttccatgttc gatcccaata tctatcgttt
     1741 ttggaaaaag ctgtttgatt tcttctggaa atgcgcttgt tgcgatatcc acatcatgga
     1801 ttggctggtt cagcaagata tcacgaacac ttccacctac aaaataagct tcatatcctg
     1861 cattctctaa ttttttcaga actggaattg ccttttggta ttccagaggt aattgtttta
     1921 atttcatttt tccaaccttt cctgcgtgtt tcttgtcctg agataaagac tcagttgcat
     1981 ggtgactgtt tgacaagtcc gtttcagagt cttcaaggcc aggaccactt catcttgttt
     2041 actcgtcctt acgctcaaag cgattatggt ctctttgatt gaatttttcc atgttttttt
     2101 caaaaacttc agttaagtcg atattcaatg aattagccat gatcatcgta acgaataaaa
     2161 catcaccaag ttcttctgct accgagttaa ctggttcaga agcctttttg cttttttctc
     2221 cataagtgtg gttgacttct cttgccaatt cacctacttc ttcagttaat cgagccatct
     2281 gagctaatgg agagaaatac cccaccttga attgctggat atattcatcc acttcctgct
     2341 gcatactgta taaagaacgc tttttatcca tctttttccc tccaaaacta tcttaccaaa
     2401 actttaacgc aaatcctaga taagagcaat tgagaaaatc gaatttccat gctacattta
     2461 ggaaggaatt tctaaatgga aattctgtga aaatacataa ttgaaagctg gaattcatat
     2521 ggtcaaacga aaaacagtaa aagaagttct ttttatcatt ataggaacaa gtatttatgc
     2581 atttggtctt gtttacttga acatcgctaa tcatttagca gaagggggag tctcagggat
     2641 caccttgatc ttaagagctt tatttggtat tgatccagct tatacgactt tattaatcaa
     2701 catcccattg attttactag gaggaaagat cctcggtaaa cgttccttgg cctatactgt
     2761 tctaggtaca ctttctttgt cattcttttt atggatctgg caaagggttc cactagaaat
     2821 caatttacag catgatctac tgatcgtttc tctacttgcg ggtttagttg caggtgtagg
     2881 aagcgggatc gtctaccgca tgggtggaac aacaggcggc agtgatatta tcgctcggat
     2941 tttagaacaa aattacggca tcactatggg gcgttcccta ttggcatttg atatcatcgt
     3001 cttacttgct tcattaacct atatcgactt gaaacgtatg atgtatacgt tgattgtttc
     3061 gtatgttttc agtcgtgtga ttgattcgat cttagatggc ggatattctg caaaaggtct
     3121 acttgtcgtc tccaacaaaa gcgaagagat tgcaccacta ctgatgaccg gtctgcaaag
     3181 aggtgtgact ttcctttctg gagaaggcgc attctctggt aattcaaaaa aaatcatcta
     3241 tatggtcgtt agctctagtg aattaactga agctaagagg atcgtccacg agattgacga
     3301 aaaagctttc ctttcaatca tcaatgtgca tgaagtggaa gggcaaggct ttacttattt
     3361 gaagccacag acaagattac tgaaaaaact ataagaaaaa agacaaagat aggctatgag
     3421 gaaagattcc acatccagct gacctttgtc ttttttagta ctatactatt tttaactaat
     3481 tgttatgttc acttacccgg ttacttttct tttgtgtctg caaaaggatc tttcggttaa
     3541 cttcatcaga taatttcaaa aaagcatccc tatctccatc ttctagtgct tgatcgattt
     3601 gagcataaag catttttagt tttgcctctt cttctttttc ttgaaaatat cgttcaatta
     3661 attcagtatc ttcttcactg actgaagaat cccaagaaga aaatggattg tcttctaaga
     3721 tagatagata ttctcttgtc tgccaagcat tttcgaatat acattccaca tataaaggct
     3781 ttttccaatt caaacggatt tcatgaaaaa tctggtctgt gtccgtaaat tcttttccct
     3841 gcaaaaacaa tttcatgggt tccccttcaa cagatagatc actgatctgc agtcctcgat
     3901 ccgcaaaatt cgccccttca acaaagtgga cattgcttaa aatcgcttca tgattggcaa
     3961 gataattcaa gatccaaaaa acttctcttc ggctaaaaga tacatgattg accatccacg
     4021 ttaaaaaatt cttcttttcc cgtacatcaa ttagcatttt tctcacctct ctgttaggtc
     4081 atctaataat gacagtgcct ccatatctcc tggttcaagt gttaaataat aagtcaataa
     4141 ttcttgcgct cttttcagct gcccttcttc tcttaagaaa aaggcatact ctttcaagaa
     4201 atctggttca tgtgatagtt catgataagc ctgttcatag tgtacagctg caacggtaaa
     4261 gtcttctaat tcattataag catgggctag gttccattct gcataaggat tagcagtttc
     4321 ttccatctga ttgatcactt gtatcacatc ttcatatctt tcatcatcca agtaaagatt
     4381 acttaaagta agtaatgttt catcttgttt gtctcctact tctaaagctt tcagtaaaag
     4441 ttcttctgct cgctgtctat catgcagacg gaaagcattt tccgaggcca ggtgataaag
     4501 ttcgacttgg taaggatttt ctttgattcc ttcttctaac actgtttgtg cttcttcaat
     4561 catttcttct tcttgaagag cttgtcctag atatagatat aacgattgat aatgcggatt
     4621 aacttctcga agttcttcca gatagttgat tgcctgctga ttttctttca attgaagatt
     4681 cgtaaaagct agctggaaca gtcgatcatc tgtcctttct tctttcagtg cttcttctaa
     4741 aggtacgata gctgcttcga attctccttg catacttaag gattgtccta aacgttcctg
     4801 catcgagatt cctgaaattt catttatccc gcttgccaac aatgtttcat aaaccactgc
     4861 tgcctctttg aaacgatcaa ctgaaaaata caattcagct agcgcaaact ggatcaatgg
     4921 ttcatcagga agcaacccag ctgcttcttt caattttgct tcactcactt cagggattcc
     4981 gataacttgg tacaaatcag ctatcgccaa taagctttgc ggatagtaat cactttgctt
     5041 atccactttt tccaggtatt caaaagctaa gtcgatctca ttatcttcga ttgcaatttc
     5101 tgctaacggg atgttcagtc cgtcattttc tgggtattgg ttgatcagtt gttcaaaaat
     5161 ctgttttgct tcgatcaaaa agccaatagc cagcaattct tcgcctagtt cagctaatat
     5221 atctgctggg tcttctttta atgctttttc taaaaagatt tgtgcttgtg acaagtcttc
     5281 atctgataaa gcttgcaaca ttttttcgct atttgtattc atttcaattc cctcttatct
     5341 cactgacgat ttgttgaaat accgtcattg ttctcaataa ttctttttcg tttggatgtt
     5401 ggtatccgtc tattatacct attttttcta aaacaagtat ttttttgctt ttttctgcca
     5461 aaaaaagaac tttttccaaa tactccatct ttgaaatctg ttccagccag tggattggat
     5521 agccaagcaa gtccagccac acaaaaaagt tttttagttg gaaatcaaaa ggatatgctt
     5581 ggagattcca cacgaaatga aacatcattc ctaaaaattt tttcatacta gcagaaagta
     5641 gatggccatt ttcggtcaga taaaaagctt gttcaaacag tttaccataa tttgacaggt
     5701 tttctgcctc taattgataa aaatagatca tttcttcgat catcccggca aacggccgag
     5761 tcgttaattg ctttttattt ggatagtttt tatacagatt ttgcaaaaaa cgatgatcac
     5821 aaatcattcc gcaacagata aaagtcaaaa agtcaatctg ctttccgtcc acctgccgct
     5881 cgctgattgt ttgatcataa acgatgcgtt ctggcagatt gatggtttgc agcactacac
     5941 gatttggtag ctgcaaaatc gtccgctgcg gtgttaatgc tcggcttaga gaacggatcg
     6001 atacagggat catccatagt gatgaatcaa gggttgtatg tttttgaaaa aaacctgcca
     6061 gatccatgat cccttcattt ccaaatccga tgataaacat ggattgattt tcgggatagc
     6121 gtttgacgaa atcaagcagg ttctttaatt ctgttaaatg attgcattga gtgttggcac
     6181 aaatatacca gtcattgttt gcttgatttg caaataacgg gttgatctta tctgcaaaca
     6241 aatcataata gcgctgattc gttaaaaata agattggctg atcaatggaa agttctctta
     6301 gctgactgac tagcgtttct ccgtaaaaca ctttcgtcaa cgcatcttct acttgataat
     6361 caagttccat acgcatccct cctcttctta tttttatttt accattagta agcaaattct
     6421 agaaaagata agagcaaagc tcacctcata acttggaaat aaataaaaat taaataaaat
     6481 acatgtgttt tctatgtatg acatacggat tcggaatctc tgtctcttat ttattccttt
     6541 tctgctagga aataaaaaga aaagcacttg tagccctaga gccacaagcg ctttttcaac
     6601 tgtcttattt aacagcgtct tttaatgctt tacctggttt gaatgcaggt actttgctag
     6661 ctggaatttc gatttcttca ccagtttgtg ggttacgacc tttgcgagct gcgcgttcgc
     6721 gaacttcaaa gttaccaaaa ccgattaatt gaactttttc gccgttagct aaagcatctt
     6781 gaatagttga aaatacagca tctactgctg cagttgcatc ttttttagtt aaatcagtag
     6841 ctgaagcaac tttttcgatt aattctgctt tgtttgccat ggatagattc acctcctcca
     6901 caagagatat gatagaaata tcatttagtt aaaactttct gagtggtcca gaagtttaaa
     6961 aagaaatatt gattaacttc aatattctgt tatcacgata tcatatgaac cgcttaatag
     7021 caaggttttt accaattttc acatgctttt tttagtcata gaaacgtaac aaacgaaact
     7081 gtctttttct tttgaaaaaa aaacgtcgat atggtaaaat gcctattttc tcctacgagg
     7141 gataattcgg atggacgtcc cttcaaaagt aaatgctttt ctgatttgat tttctaagaa
     7201 acgttcataa gaaaaatgca tcaattcctc ttcattgaca aaaacgacaa atgtaggtgg
     7261 tttgacagcg acttgtgtag cataaaaaat cttcaggcgt tttcctttat ctgttggtgt
     7321 tggattgatt gctacagcat ccataatgat atcattcaac acagccgaag agatacgaag
     7381 attctggttc atacttactt gttcgatcat ttcaggcaga cgttccaacc gttgtttagt
     7441 cactgcagaa acaaaaacga ttggagcgta atcaagatac cggaattcat cacgaatctc
     7501 ttgttcgaaa tcacgcattg tattggtttc tttctttaca agatcccatt tattcacgac
     7561 gataataatt ccacgaccag cttcatgggc gtaacccgct actcgcttgt cttgttcccg
     7621 aatcccttct tcagcattca gtaccatcaa aacgatatca gaacggtcaa tggcacgcat
     7681 tgcacgcatc acgctgtatt tttcagtatt ttcgtatact tttccgcgtt tacgcatacc
     7741 agctgtatct atcattaaga atttctgacc actttcagaa acgaagtggg tgtcgattgc
     7801 gtctcgggtc gttccttcaa tatccgaaac aattacacgt tcttctccta aaatcgcatt
     7861 gatcaaagag gatttaccaa catttgggcg accaatcaaa ctaaacttaa tcgtgtcgtc
     7921 gtcctcttct tcagtctctt cagagaaata cttcactgct tcatctaaaa tatcaccaat
     7981 ccctagtcca tggcttcctg aaacaggata aggatcgcct agccctaagg aataaaattc
     8041 atagatatcg tttctcattt ctggattatc tactttattg actgctaaaa tcactggttt
     8101 attacttctg tataaaattt tcgcaaccat ctcatctgcg tctgtcacgc cttcgcgtcc
     8161 gctgactaca caaatgatta catctgcttc ttcaatcgcg atttctgctt gatgcttgat
     8221 ctgatccata aaaggttcat cgccaagatc aattccgcct gtgtcgatca cgctgaattc
     8281 tcgacctaac cattcacctt ttgcataaat acgatcacga gttactccag gagtatcttc
     8341 gacaatcgag atacgctcac cagcgatgcg gttaaaaatc gtagacttac cgacgttcgg
     8401 gcggccaacg attgcaattg ttggatttgc catattattt cctcctcaca agtttccaat
     8461 ctcacttagt ttaacgaatg cattgccaac tttcaagcat agattgtcaa ttaaacgaag
     8521 tatttttatt ccttattgaa atactaatcg cttttcaaaa gaaaaactga gacaaaagca
     8581 gctgctttca tctcagtcac agatacaaaa tcaattattt ttcagaatca tcggttgctt
     8641 tttcttctac ttcttctttc aacgcatcac ctaaaatgtc tcccatagta aatccagtat
     8701 tttcttctgg taattcataa gactcaacat tttttggttc ttcttctgaa gctggttttt
     8761 cttctaaagc tttgatactc aatgcaatac gatgttcttc tggatggatc tctaatactt
     8821 tcactttcac ttcgtcacct tcatgaagca cttcatgtgg tgtcgcaata tgtttatgtg
     8881 aaatttgaga gatatggact agcccttcta ctccagggaa cacttcaaca aaggcaccga
     8941 agcttgtcaa acgtttaact gttccgtcta atactgtgcc aactggagct tttgtatcga
     9001 tttctgacca aggtccaggc tgtgtgtctt tgattgacaa ggaaacacgt tcttgttcag
     9061 gattgataga caacactttg acatttacat catcattgac agccaataca tcacttggtt
     9121 ttgctacatg actatgtgaa atctctgaga catggactag accgtcaatt ccgcctaggt
     9181 cgacaaatgc gccaaaatca gtaagacgtg caacacgtcc actgagaaca tctccctcat
     9241 ggatagttga aagaatttct tttttctttt cttctctttc gttttccact accgctttat
     9301 gcgataagat caaacgattt tctgatggtt cgatttcgat gattttaaag ctcaatgttt
     9361 tgcctttata gtctgaaaag tctgcaacaa aatgatcttc caccattgat gcagggacaa
     9421 aaccacgtac accgacgtca acgactaaac cgcctttgac aacatttgta acaggtgctt
     9481 cgatgacctt gccctcttgg aaatctcttt cgatatcttc ccaaactttt ttagcatcta
     9541 aacggcgttt tgaaagtagg tagctgccgt tttctttatc tttgccgatc gtactgataa
     9601 cgactaaatc taatacatca ccgactttta ctacttcgct gatatcttct accggcaaag
     9661 ttgataattc ttttgccgga acgactcctt caactccagc cccttcgatt ccgacaatca
     9721 cttgtttgtc ttcaatgact aaaacttcgc ccttaacaac atcgccaact tttacttctt
     9781 gtacgctttt cattgcgtct tccattgact ggttgttctc ttgattttga tcaaattctg
     9841 tcataacact tgtcctccta accaacattc tgccgtaagt acggtactgt ttttagttta
     9901 attgattatt caaaaaaaat aaagcgattt cgttcgtttt cct
//
LOCUS       DAHHZJ010000109         9943 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.110, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000109 DAHHZJ010000000
VERSION     DAHHZJ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.110"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(35..232)
                     /locus_tag="NBL02_002044"
     CDS             complement(35..232)
                     /locus_tag="NBL02_002044"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HCD2572721.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(378..1256)
                     /locus_tag="NBL02_002045"
     CDS             complement(378..1256)
                     /locus_tag="NBL02_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCD2572722.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1295..2413)
                     /locus_tag="NBL02_002046"
     CDS             complement(1295..2413)
                     /locus_tag="NBL02_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HCD2572723.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2410..4509)
                     /locus_tag="NBL02_002047"
     CDS             complement(2410..4509)
                     /locus_tag="NBL02_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HCD2572724.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4560..5381)
                     /locus_tag="NBL02_002048"
     CDS             complement(4560..5381)
                     /locus_tag="NBL02_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCD2572725.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5378..6187)
                     /locus_tag="NBL02_002049"
     CDS             complement(5378..6187)
                     /locus_tag="NBL02_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCD2572726.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6208..6702)
                     /locus_tag="NBL02_002050"
     CDS             complement(6208..6702)
                     /locus_tag="NBL02_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCD2572727.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6714..7202)
                     /locus_tag="NBL02_002051"
     CDS             complement(6714..7202)
                     /locus_tag="NBL02_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCD2572728.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(7279..9897)
                     /locus_tag="NBL02_002052"
     CDS             complement(7279..9897)
                     /locus_tag="NBL02_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCD2572729.1"
                     /translation="MKKSAKDRVFDELIRLTGNMSSVTAEKISKNLGISRQNASHYLT
                     RLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQINEAFHSVVGHDGSLREVIQKCIAAV
                     KYPPNGLSVLINGATGVGKSFLATKIFEYAIHEQIIEKDAPFAILNCADYADNPELLS
                     ATLFGYKKGAFTGAEKDTDGLLATANNGYLFLDEVHRLSKENQEKLFLFIDTGNYRPV
                     GENVNWHSAKVRFIFATTEKGENYLLDTFDRRIQISVMLPTFEDRPIRERLELIQLFF
                     QNEADVLQKDIIVSREALSLMLSHPFSGNIGKLKNIIKISCADVYSRTQEEPLTIGKN
                     EILIQLNMLESEVESLPIASLLIKKNQKIKTDRIKPFDYDSILEKITQLINEGNFNEN
                     FSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMFRKVWVSVLGKTYGLTTSGPVAEIISR
                     SYVISPSYKTDLTGINRTLAANLPRTTYLCNQFLEHLPPLSEKDENFLRLLIVVTLSD
                     YVDESIELKGLLLAHGDSTASSIQAVVNQLCGNYVFEALDMPVESNVSEIVESTRNFV
                     KRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGELLLLNNLTTSIALDIGLKMTMNAPFK
                     QIAEDAADKYSINVQYFEGFSQSSNIIISCMSGLGISDKLKDVFRHHLSDESIEVFTK
                     DYRELRELIDQNDETYFDKTKLVITTSDLPRSFSIPNINVYDILDGDGAANLEHILSP
                     EMKPAEFNRLIQELVKFFSLEGIADRLNFLNPTIVMSEVETVISKYENYYHFTLNGRV
                     KLNLYMHTALMMERLFLARTEKSEEPNYDLKPDEDEFYYVSRSIFQSMEMKYNFKVNA
                     YELSLLYELLGPYIQK"
BASE COUNT         3019 a         2248 c         1646 g         3030 t
ORIGIN      
        1 attacgattt attttatacg gctgaaaaaa tctatcacca aaaaaattca aacattaagc
       61 caaggattat gaagtcaaca tttcgaaaaa tgaacataca ttggtaagca actataacta
      121 ttaccacaga cataaagtaa cacaatatat tactatttaa gtgtacgcca ccagtgaata
      181 tcctcaaggt atacagcgca caacaaaaag aaaaaaggaa acgcagaagc atttcctttc
      241 agtgatttaa tgaaaacaac acgatttgac ttagagcctt tttatttgaa ttcattttcg
      301 ctttatccaa cttcatttga tcatttgatt actcggttaa atacttttga catgagtatc
      361 aatgaaacat tcattaatta tggttgctta tttcttaccg cttttctggc caatgctaat
      421 ccgccgatta ttccggcatt atcacctaat tcgggcgtaa taatgtagct atcaattggt
      481 ggtatttcaa catatccatt tataagctca acaaatttat cctgaacttt tttcttcatg
      541 tgttcttgtt tcattacacc accgccaaaa ataattctat ctggcgaaaa catcagtgtt
      601 gtattgtatg cacattgggc aaggtaaaag gcttcaatct gccaaaacga atcgtcagcc
      661 aataaattct ggccttttac acccaaccgt ttttcaatcg ctggtcctgc tgccatcccc
      721 tctaaacaat cttggtggaa gggacagttt cctgaaaaat tgtcctttgg atgtcgcttg
      781 atcaacatat gccccatttc cggatgactg aacccttcca cgaaagtccc attgaacaag
      841 gcaccacctc cgatacctgt tcccacagtg aagtatgcaa cacctgctaa ccctttgcca
      901 gaaccagcaa catattcacc atagcaagcc gcattgacat ctgttgtcca ttcaatcgga
      961 atattgaaat attgctttag cgttcctaca aaattgaaat tttgccaagc aagttttggt
     1021 gttgatgtga tatgaccgaa actcgttgat ttgcattgaa tatcaattgg accaaagcag
     1081 ccgaccccga tgcctgccaa ttgttcctta aaaggtttaa aaaaatcaat gacctgcttc
     1141 attgtttcaa ctggagttgt tgtgggaaaa ctgactcttt ccaaaatcgt taattcctca
     1201 gtaccgactg cacagacaaa tttagtccct ccagcttcaa ttgccccata tatcatcata
     1261 ggttcctccc ttcgaatatg tgaaaaacac ttcattaacg cggcaagcgt tctcgatcag
     1321 tgaaatgacg tttagtattt cctgaagctt ctgttgaata ccagtaagcg acactagcaa
     1381 catcgtcttg ccgctcaaat aagcgaacat catcgttgcc gatttgttgt aaagtgaccg
     1441 ataaatcttc ggtaaaacag atggggtctt gaagatgcca acgataaaga ccatgtcgtg
     1501 gcattgcgtc gtcaccaaag gcatgaacgg cattcaattt tcccgtttca aaacgatctc
     1561 ttgtggcatc tctatttgat tggtaaggat accctaaaaa ggctgttgaa tagtttttcg
     1621 catgtggacg tccgaactca tcgtactcat gaaaggccca cgccccgcca aaataatctt
     1681 ctgaacctgt tgaggttacg gtcggatact cactatcccc atcaatgtag aatttaaact
     1741 cgccttcgcc ccaccaataa cgttctaggc aggtaagcgc aaggtaagta cccacgtaat
     1801 agccttgacc ctttaatcga tcaattaact tataatcctc ctgtaatttg gtaatgcgtt
     1861 cccgatgcca atttgcgtga aaatataggc tattttcttc caattcgtcc accaaagcgt
     1921 aatttatcgt ataaaaaaat gaggacatat ccttcggatg taaattctcg atcgtaattt
     1981 tagcggatgt ttggaaaggc atttcgaaat aggaattaaa gccccctgta ggattaacca
     2041 ctatcggcaa cgagttcacc tcgcaacgct ctccaaaccc attacaaaag aaatccccta
     2101 aaggactttc aacagcaggg ctatccgacc catcccaata aattcttaag attaaatcac
     2161 gtaaaacaaa actgccaaat tctgttcgat cacgaatcgt catccacata tgacgaatta
     2221 ttcccgttcc agtaatatcc gcaatcacag tttcttcacc agaagtcagc ggaattgcgc
     2281 cacgaccttt gcggcttggt cctagctcgc ttggcaacat cgcagcttta cctttctcgc
     2341 ctgttggatt ttcgggagtg attgctcgac tctggatttt tttaaattgg gtaatatttt
     2401 ttagtatact catctttttc tcctttgaat gtgaatcgtt tttccctttt ctacgtgaag
     2461 aatttttttc gtatcactta ttttcaatgg cttttctgac aaatttgaaa tactcaactc
     2521 ggattgcgta cattcaatcg aaatgaaggc ttgaccgaac ttcaaattct tccaagaaaa
     2581 cgttaaatta tcaacaggat caatttgcaa tactcctttt ttgaatttca atccgaaata
     2641 ttgactgatc attaggctga caaaaagacc cgatgcccag ccgcatttgc cgcagccaaa
     2701 gtctcgcacg acttctcctt gttgagcctc taagcgatac ggccaccacc accacgaacc
     2761 gtctaaatct gccaaacaaa ttagttcttc taatcgggtt acgaaagctt gagggttgtg
     2821 cagctcgctc attaaaacag tgataaagcc aggaaaagtg gcgccggatt ctaatcccca
     2881 agtgatgcct ttgatttcct cgctataggt tggattgaaa gaactagcag aaaaggccat
     2941 ggtgttctga tataaatgat ctttattatc taaaaattga taaaaaggca tcaacgtagt
     3001 gtctgattct tctccatcat gcatcaataa attaattttt ccatccacaa tcacatcact
     3061 taaaaagatt aacccttgct caagaatcgg tttttgatag tgcttcactg aataggttgt
     3121 tttctcctca tcaccgattc cctccaaaaa ttgctcacca aaaggaccat cgatagtcat
     3181 ttgtttacaa attgattcct ttagtttaca ggcaatgttt ttgtatcgct tgctaagact
     3241 cagttgttct agggcttcaa aaatttctgc aagtccaata caggccttcc aaagacaaag
     3301 attgctgccg gtatgatact ttccaagtgc aaaggcgtct gataaccaaa cagatggaaa
     3361 aagcattggt tcccctgacc gacgttgagc taatatgtcc tcaataattc ttgaagcatt
     3421 cagtagaacc tgcggacacc gctcaaagaa ttttgtatcc ccagtatgtt caaaataaag
     3481 actagccagt aacgcactgg ataacgaatt actcagagaa tgattgatac ctcctgtaaa
     3541 ttttgaacca ggaaacttaa cgccatattg atcaaaccat agaaccgttt tttgacataa
     3601 ctccggttca aacatggtga atggcaatga tgcgtaatac atatccttat tccaaattcg
     3661 attagtagca ggatagctgc cccaattgct gccgacgacc tgtccctgtc gattcatacc
     3721 gaaactagaa aagctttgat aaagtgcacg acgatagaga tggacaactc tatcgtttgg
     3781 cattgacagc tgcccaaata gctgagcaaa atagttcaat gtttccttca gccatttttc
     3841 gatatcagat tcctgaaaag tcattacttc ttcaaaagca tttgggtcca caaaagcaat
     3901 ggcgaagctt gccacttcct gttgtgcgat cgtgtcctta tttccttttt gagaaatcaa
     3961 cacattttgc tgatccgaat acttctcttg atacgaaggc acatctttca ctgaaacttg
     4021 aaggctttcg tgtgtttcat tcacaatgta aacctgttga acaagcatac ttaaacgctt
     4081 attagccagt atgggagcaa aggatactac aatgacctta aacgtcgaat aatgatgaat
     4141 atatctaggc aaacagtctt gaatcacatc gacttcaata cgatgatccg attctgacaa
     4201 atggtagaca atcccgttta cttcaacctt taaggataac gaattggttt gagagagctg
     4261 tttattgacc caaacacctg gtttttcctc agttagtaaa ttttctcgat aaaaggtcaa
     4321 cgtttgaatc gtcccgcaaa catcaaggtt tgctaatgtc ttttgattac ttacatcgaa
     4381 ggtcaatccc tttacatcag gaatattccg atagtagctg tattcgtttg aagtagtcac
     4441 atttccgtcg tttaactcag cattaaaaaa tgggtgtttg ggtgaataaa ttacttcatt
     4501 aatagtcatc ttttccctcc ttgctaaaaa aactgcagcc tagtagctgc agcttcctac
     4561 tatgcgattc caagtaatga caataaaatt cccaagacga ccattccaaa aataattttg
     4621 atgacgccga cttttttatc tagtaatttg aaacagctca atgttaccaa taacggtaca
     4681 attcctttaa aaatactatc gaggatttct tggacattca ttagttcttc gcccttcatt
     4741 gaaaagctca aaacggtatt gaatttgacc atactgatcg tcattgcacc taccatgatc
     4801 agacccaaga tactagcagc ttttgtcaga atttcaatta acccgttcgc atacagcttt
     4861 tcaatatatt tagagcctaa tgaatagcct aaaacgccgc catagtatcg catcaagtaa
     4921 ttaggtatat tgaacataac taaaaagata attggtgcca aaatatttcc ggcattggct
     4981 aagttgatcg ctagtcccgc ggtaacgaca cgccaaactc cccagaacaa tgaatcgcca
     5041 attccagcca atggtcccat caaactcgtt ttgatcgcgt tgatactttc cacgtcaaaa
     5101 ccagcttttt cagaattttc cttctccatc gatgccgcaa tccccatgac aaaggacgcg
     5161 caaggaatgg tcgtcgaaaa atactgtgta tgacgttcca gcgccgcagc tcgatcttct
     5221 tcacttttgt agaaatgatt aatgaagggc atcatagaat aggcgaaacc accgccgccc
     5281 atcgtcaccg gattcaccga agcatacata gtcatcgagc gccaaaacat cttccgaacc
     5341 aattttttct catctttaga aattccatca gctaatttca ttgaaagaaa tcctcctctt
     5401 cttggtcaag cgttgcattt acagcagcat cgctttgaac tgcatgaaca gatatatttt
     5461 ttagttgtag atctctttgg gctataacaa ttgcaataat cgttccaact gcggcaattg
     5521 caatcaatgg taactcgaga tacgccataa caacggcccc aaagaaaaac cacacagcaa
     5581 tatttttact ccaaagcatc cgtaacagca tcgccatccc aactgccggc agcagatttc
     5641 cagcgactgt taatccgttc atgatgacct ctggaatatt atccaagacg cttttgacaa
     5701 catctgctcc aaataaaacg gcaaagaatg gaataagagc ataaagcgca tagtggacaa
     5761 accacataaa gaagtgcaga ttgatgattc ctttttgatc accagatttt gcaaatttga
     5821 aaaatttagc agccatcggt ccacatacaa aagaaaaaat catattcatt aactgtaatc
     5881 ctaatactgc tagtggaaag acaatgggaa taatcgcatc cacgtttcct cccattttga
     5941 ttgcgaacgc tgtagccagc ccagcagcca aagaaggctc cgccgtacta gagattccga
     6001 tattgacgac acccataaag atggcttcta gtgacgcccc gacaatcaac cccgtctgca
     6061 aatcccccat caacgctcct gctaacggag caacaacaat cggacgactc agcatggaca
     6121 tcccgaacat ttcatgtcca gcaactccaa taaaaacggc caaagcgact acaaatgcat
     6181 catacataat ttttctcctt cctttttcta aaaatcactc aaaatagaga tagctgaacg
     6241 tttttccatc gccggtgtag gctgaaaaac ggtatctgga taaagttcaa ctaattcttt
     6301 taagtcttga atttctgacg tcgttaacat cacttccttt gataaggttt tcttatcttc
     6361 cgatggcggt gtcattttcc cagcattccc cacgttcatt aattcgattt ccgttgggaa
     6421 agctttcgca acttttaccg catctttgac attagggact aaaaccatca agcgcatgtc
     6481 cttcgcccgc ggatctttta acaaatgaat cgcgtcatca actgttttaa taatgacttt
     6541 gacattcgat ggtgcagcca tcttcaatgt catcttttga atatcgttgg tagccgcctc
     6601 atcgcttgct actactaacc cggttaaatt tagactcttt gtccaagtca tcgctacttg
     6661 tccatgaatt aatcgttcat caattcgcac tgctttaatc atttttcttc ctcctaaaaa
     6721 aaatcttcct cttcatttac tacatgtacc tcatcaagaa tggtcatctt taattcctgt
     6781 tggcatttat ttaccagttc ctgaatatcc tcctttgtta attccttggt tgccaatact
     6841 atttcgagca gaattggcag attaaagcca gacaaaatag ttacagcaat ctctttctcc
     6901 aaaaaggttt tggtaatctt ctgattgaca cttccgccaa ataaatcggt aaaaacaaaa
     6961 atctgatcct gctcgttcac ctggtttaca aatacattca actcctgttc aaaatcacac
     7021 tcatccacat aagcattaat caccgtaatc tcttgaacct tccccgtcag taattgaata
     7081 ctgctaagaa agcctgaagc taagtgaccg tgagtcgcta ttagaatttt cttcatatgg
     7141 ttaattcctt tctttctgtt cacttttata taagcaattc ctgtgccaac ttttaaaaac
     7201 attggtttaa tcacgttaat tcacattaaa aaaagtgtct aacctaaaaa aaacagatta
     7261 gacacttttt tagacacttt atttttgaat atacggtcct aataattcat agagcagcga
     7321 aagctcataa gcattgactt taaaattata tttcatctcc attgattgaa aaatacttct
     7381 agaaacataa taaaattcat cttcatctgg tttcaaatca taattcggtt cttcagattt
     7441 ttctgtccga gccaagaata aacgttccat catcaatgcc gtatgcatat agagatttag
     7501 ttttacacgg ccatttaaag tgaaatgata gtaattttca tatttagaaa tgactgtttc
     7561 cacttcactc atcacgatcg ttgggttcag aaagttcaaa cggtccgcaa ttccttccaa
     7621 cgaaaagaat tttactagct cctgaatcaa gcggttaaat tctgccggct tcatttcagg
     7681 actcaaaata tgctccaaat tggctgctcc atcaccgtct aagatgtcat aaacattaat
     7741 attaggaatt gaaaaggaac gtggaagatc cgaagtcgtg atgaccaatt ttgtcttgtc
     7801 aaaataagtt tcatcatttt ggtcgattaa ttcacgtagt tctctgtagt ccttcgtgaa
     7861 aacttcgatg gattcatccg ataaatgatg acgaaaaaca tctttcaatt tgtccgaaat
     7921 ccccaaaccc gacatacagg aaatgataat gttgctactt tgagaaaagc cttcaaagta
     7981 ttgaacattg attgaatact tgtctgctgc atcctccgca atctgtttaa atggcgcatt
     8041 catcgtcatc ttcaagccaa tatcaagtgc aatggaagtc gtcaaattat tcagaagaag
     8101 taactcccct tttaaatcgt tcttgatctg tgtgtaaatt tgatttaaag agcccatatc
     8161 tacgatcagc actagactat ctgtattcac ttgccgtttc acgaaattcc ttgtcgattc
     8221 gacaatctct gacacattac tctctactgg catatccaac gcttcaaaaa cataattgcc
     8281 gcataattga ttcactactg cttgaatgct gctagccgta ctatctccgt gagcaagcaa
     8341 caggcccttc agctcgatgg attcatctac ataatcagat aaagtcacga caataagcag
     8401 tcttagaaag ttctcgtcct tttctgagag cggtggtaaa tgctccaaga actgattaca
     8461 taagtaagtc gtccgaggta gatttgcagc taatgtacga tttatcccgg ttaagtctgt
     8521 cttatagctt ggggaaatga cataggatct gctaataatt tctgcaacag gtccactcgt
     8581 cgtcaatccg tacgtttttc ctaaaacgga tacccagacc tttctaaaca tctgaatcat
     8641 caattttcta ctcattgata aaaaagaatc cttatcaatg tgatgcacca aatgctgtac
     8701 ttcattcttt atcgaagaaa aattttcatt gaaattacct tcatttatca gctgagtaat
     8761 tttttccaaa atcgaatcat aatcaaacgg tttgattcga tccgttttta tcttttgatt
     8821 tttctttatc aataaactag caatcggcaa gctttctact tcggattcca gcatatttaa
     8881 ctgaatcaag atctcatttt tcccaatcgt taatggttct tcttgtgttc gactatacac
     8941 atccgcacaa ctgattttaa tgatattctt cagcttacca atattcccac taaagggatg
     9001 agataacatt aaagacaagg cttctcttga aacaataata tctttttgta aaacatccgc
     9061 ttcattttga aaaaataatt gaatcagctc taatcgttct cgaatggggc gatcttcaaa
     9121 agttggcaac ataacactaa tttgaatacg ccgatcaaag gtgtctaata aataattttc
     9181 acctttttcc gtcgtggcga aaataaaacg aaccttagca gaatgccaat ttacattttc
     9241 accgactgga cgatagtttc cagtatcaat aaatagaaag agcttttctt gattttcttt
     9301 cgataggcga tggacctcat cgagaaaaag atagccgttg ttggccgttg ccaataaacc
     9361 atcggtatcc ttttcagccc ccgtaaaggc ccccttcttg tatccaaaca gtgttgccga
     9421 aagcagttcg ggattgtccg cataatcggc acagtttaag atagcaaatg gtgcatcttt
     9481 ttcaataatt tgctcatgga tagcgtattc aaaaattttt gtggctaaaa aactttttcc
     9541 aacacccgtg gctccattta ttaatacact gagtccgtta ggcggatatt tgacagcggc
     9601 tatacatttt tgaatgacct ctctcagact tccgtcatga ccaactacag aatgaaaggc
     9661 ttcgtttatt tgctccgtat tatctacaac cgcatattca tctaaaggct tccacaaaac
     9721 aggctttccc ggtaatttct cgaccttttt atcttccact aatctcgtca aataatggct
     9781 ggcattctga cgagagatac ctagattctt cgaaattttc tcggcagtga cagaagacat
     9841 attccctgtt agtcgtatca gctcatcaaa aactcgatct ttagcgctct ttttcatttt
     9901 ttcctccagt ttaatgtgtc taaaagcata ataaactatc ggc
//
LOCUS       DAHHZJ010000110         9929 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.111, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000110 DAHHZJ010000000
VERSION     DAHHZJ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.111"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(150..2270)
                     /locus_tag="NBL02_002053"
     CDS             complement(150..2270)
                     /locus_tag="NBL02_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCD2572730.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2500..3678
                     /locus_tag="NBL02_002054"
     CDS             2500..3678
                     /locus_tag="NBL02_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HCD2572731.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3694..4452
                     /gene="aroD"
                     /locus_tag="NBL02_002055"
     CDS             3694..4452
                     /gene="aroD"
                     /locus_tag="NBL02_002055"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCD2572732.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4475..4960
                     /gene="ybaK"
                     /locus_tag="NBL02_002056"
     CDS             4475..4960
                     /gene="ybaK"
                     /locus_tag="NBL02_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HCD2572733.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(5031..6284)
                     /locus_tag="NBL02_002057"
     CDS             complement(5031..6284)
                     /locus_tag="NBL02_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCD2572734.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6401..7750
                     /gene="gdhA"
                     /locus_tag="NBL02_002058"
     CDS             6401..7750
                     /gene="gdhA"
                     /locus_tag="NBL02_002058"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCD2572735.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7862..9208
                     /locus_tag="NBL02_002059"
     CDS             7862..9208
                     /locus_tag="NBL02_002059"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCD2572736.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9516..9902)
                     /locus_tag="NBL02_002060"
     CDS             complement(9516..9902)
                     /locus_tag="NBL02_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCD2572737.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3172 a         1800 c         2033 g         2924 t
ORIGIN      
        1 ttaaaaccag aaaatatttt gtaaaaagca agaggttgtg aagaagctgc cctattctat
       61 taccgtagat gcaagctttt gaacactgtt tattcgaaaa gaggagcagc gacaataatt
      121 ttgtcgctgc tccttttaaa tttattttat cattcggaag aacttgtggt cgttccgtga
      181 agttcttggt acgtttgcgt tttataaaga tcaacagttg attgtccgtt gtgttcacta
      241 aagacactag ttgacttgtc accaagagat ttttcagctt cttttaattt ctctaagcca
      301 ttcttgtagt cataatcact ggtatcaatt gggtctaatc cactattcgt atagaaccga
      361 agaagatcac cattattgat ttggtcagaa acagctaact gtttatcaac tttttctttc
      421 agtgaatcaa tttcattctg tacttcctca gtaggttcag taatagcttc accagtttcg
      481 ttactataga tcgtaccacc gtagtaagta tattttggtg taacaaaatc accatcacgg
      541 aaagaaacaa gctgttcatg atctttcgag aatagatctt gtcctaactg aatataattt
      601 tttgtatcta cacctagtaa gtgaagtaat gtaggcaagg catcaacttg tccgccgtaa
      661 gtgtgattga ttccgccctg tgtttgtcca gggatatgga tcatataagg gacacgctgc
      721 atttgtgcat tgtcgtagtt tgtccaagta gaacttgttt ttccaactaa ttcagctaga
      781 tttttatttc ttgaattcga gacaccgtaa tggtcaccgt aaaggacgat gactgaatta
      841 tcatacaagc cgctttcttt caaataattg aagaattctt gtacggcggt atctaaataa
      901 ttggctgtcg caaagtaacc gttgatcgtt tcatcatttg tattggctaa cgggaatcct
      961 gcttcgtcat tggtgaattt tgaataagga taatggttag atacagcaat gaattttgaa
     1021 taaaatggtt gttgcagacg ttccaagtat tgtacggatt gttggaaaaa tggtttatca
     1081 tgcaacccgt attggaacga attgtcatca ttaacatcat aataagaaga atcaaagaaa
     1141 taatcatatc ctaaattctt gtatgtctcg tttcgattcc agaatgtacc agcgttccca
     1201 tggaataccg cactaatgta accttgtgtt tgacttaaaa tatcaggtgc agcttggaaa
     1261 gtattttttc cgcctaattg tgtgaacaag gagccttgat tcaagccaaa caatgaattt
     1321 tccatcagcg tttccgcatc gcttgttttt cctgctttta cttgatggaa aaagttgtca
     1381 aaactgaacg tgctgttgct gtggtaaaga ctattgatga aaggtgtaac ttcatgctgg
     1441 agaccattct catctgtcaa tttataatcg atcaggaatt gctggaagct ttctaaatgg
     1501 atataaatca cattttttcc tctagcgagc ccgaacaaat cgtcatttgg tgctgcataa
     1561 tgttctttta cataattttc tacttctttc atatcattgg gacttgcttc tgcccgcact
     1621 tggtttgtct ggtaagtttg aacaccgtca taaaccatga aagcattcaa gccaagatat
     1681 ttgaccaaat aatcacgaga gaatgttcga gtcagcaatt ctggacgatc agcttccgcc
     1741 ataaaaagat ttcctgagaa aatcataact gctaatgtag agatcgcaaa agccattctt
     1801 gctcggatcg ggcgagggtc caacttgatt ttttttgcaa tcagcagaac agtcaagata
     1861 ataaaatcga tccaatagat cacatcatat gggcgaaaca gtcgaatggc actttctccc
     1921 agaccactcg ctactttccc agcacctaaa atcgtgttga tcgtaatgaa gtccgtgaat
     1981 tctcgataat aagtgacatt cgaaaaaaca agcaatgtca aaagaaaata aatgaccatc
     2041 atcgtgatat aagcaactct agggcgtctg atataaagag caattccaag taataacaat
     2101 gtagttgcaa tagggttgat aaacaagata aagtattgaa tgccactttc gattccgaga
     2161 tggaaatcaa ctaaataggc aaaaatcgtt ttcaaccaaa gtaaaacgac tagtaaagaa
     2221 aaaaatccga gcctcttatt caaaaaggca ggtgttttta tacatttcaa gataatcttc
     2281 ctttctgcac ttcttaatca cagaagtgtc ctgaaaccat gttacacagc tcacttattc
     2341 tcatttattt tactagtatg acaatatagt gtcaattgaa accagaagtt tatgctgttt
     2401 cattaaggtt ttctttaagc ttcatgaaaa aatgagcagt aagacaagtc ctatgttttt
     2461 tgttatactt gggaagagaa agctaaagca ggtgaaggaa tgaaagtaaa agtaaaaaaa
     2521 agagcaagca atcgattgaa aaaaggatat ccactgatcc aaaaagaaga ccttgctcat
     2581 ccccaaaaag aaaccacgca atgggtggaa ttagttgatt cgcaaaacca atttatcgca
     2641 aaaggatatc taggaaagca gaataaggga atcggttggg tattgagcca gcagaacatc
     2701 ccatttgatc aatctttttt tgaagaattt tttgtgaagt caaaagaaaa aagaactttc
     2761 tttttttcag atgattctac taccgctttt cgtatattca atggagaagg agacgggatc
     2821 ggcggtctga ttgttgatta ttatgcaggt tatgctgttt tttcttggta taacgaaacg
     2881 ttatatgcac atcgtgaatt attgattcaa gcttttcgtg aaatctaccc ggaaatcata
     2941 ggaggctatg agaaaattcg atttgaatca gtaaatctgc ctgagagcca gttcatttac
     3001 ggaaaagagg caccggaacc cttgatcgta aaagaaaacg gagtaaaatt tgccgtttat
     3061 ttaaatgaag gattaatgac tgggatcttt ttagaccaaa aagaagtacg gggatcatta
     3121 gcagaaggtt tcgcttctgg gaaaacagtt ttgaacatgt ttagttatac cggcgccttt
     3181 tctgttgctg ccgcaatggg aggggcaatc gaaacaacaa gtgtcgatct agcaaaacgc
     3241 agtttaaaaa aaacacagga acagtttgaa gtgaatggtt tggatgtcga tcagcaaaaa
     3301 atcattgtta tggacgtatt cgagtatttc aaatacgcca aaagaaaaca atttttatat
     3361 gacatgattg tattggatcc accaagtttt gccagaaata agaaaaaagt ctttcgcgta
     3421 gcaaaagatt atggggaact ggtcaaggat agtctggata ttttagcaga agacgggata
     3481 cttattgcgt ctacaaatgc tgcgaatgtc tcgatagaac aattccaaac aatgattgaa
     3541 gaggaattcc atgccaaaaa tgtttctttt gaacaacttg ctttatatcg tctgccaaaa
     3601 gatttccaga cgacggatac ctttttagaa ggcaattatc tgaaagtgtt tgtttatcaa
     3661 attaaaaata accattagaa ggagcgatgc agaatggttc gggtaaaggg attgaatata
     3721 ggaagcggta gaccaaagat ttgtgcgcct cttatcgggg ccgtccgaga ggaagtatta
     3781 cgtgaagcag ttcttgcacg tgaagcaggg gccgatttag tcgaatggcg agtcgatcat
     3841 tatcgtgaag ttttttcttt tgatgaagta gcagaaacat tggttcttat ccatgagatg
     3901 cttgatggac tgccgttatt atttacgttt cgtaccttag ctgaaggcgg agaacaagac
     3961 atatcgatcc gtcagtatcg cgacctttat gaacatgtga tacacacagg attagtagat
     4021 atggtcgata tcgagttgtt caaagtagaa agtctaggaa aaagactgat agaggaaatc
     4081 aagtctttaa acattccttt aattatcagt agtcatgatt ttaaagaaac accagcagac
     4141 cctgtcttgc tttaccgatt gaatgttatg gaacattttg gagccgatat cggaaaactg
     4201 gcagtaactc ccaataatga acgagatgtt ttgcggttga tggagttgac tagacgggca
     4261 aatgcttttt tctccatgcc tattattacg atgtccatgg gaaatcttgg taaaatttct
     4321 cgtctggttg gcggaacgac gggttccgtt atgacatttg gttccttaag cagcgattct
     4381 acttctgctc caggacaatt accggtcaaa gcattgaagc aaattattca attattaaat
     4441 gaaccgttgt agaacaagtt tgaggtgata ttttttggca aagaaaaaaa tagtaaaaac
     4501 gaatgcaatc cgtatggtcg aacaaaaaaa gattccctat acagagcacg aatatgagtg
     4561 ggacgaaagc cacctaagtg cgtcatcggt cgctgaacag cttccagaaa gccaatctcg
     4621 tatatttaaa acactggttg ctgtgggcaa tgtgacgggg cctcttgttg ctgtgatacc
     4681 gggagaagcc gaattgaatc taaaaaaatt agcaaaagtt tcagggaata aaaaagtaga
     4741 aatgctccat ttaaaagatt tggaagcaac gaccggatac atacgtggtg gctgctcgcc
     4801 tataggtatg aaaaaactat ttcctactta tttagatcaa attgcagaaa gctatgaaca
     4861 aatcattgtc tcggctggca gaagagggct acagatggag ttagcacctc aagatatctg
     4921 tgcattaacg agcggacaat ttgcagatat aaaacaataa atcatacgta taaaagaaag
     4981 aaggaggccg ggatgttgtt tgtccagtct ccttctttct tttttataca ttatgctctt
     5041 tgcccggcat tcattcctgc tacatgccct gtagcgaaag ccgctgtgat attaaagccg
     5101 ccagtataac cattgacatc cagtaattct cccgcaaaat acagaccatc aatgatttta
     5161 ctctccaatg ttttaggata aatttccttt agagaaaccc ctccgccagt tacaaatgat
     5221 ttctcaatag gaaaagtttt gttgattgat agcttaaact ctttgcatag ttgaacaaaa
     5281 tcttgaattt gtttttgaga ggcttgttgt cctgtcagat gatccatttc aagccgctca
     5341 agaaagaaaa ctaacaatcg ctcaggcaat ataccatgcc aagcattgac caagttcttt
     5401 ttggttgctt tacttttttc tgtcaatata tgaataagct cttgtttggt ttgtgtaggg
     5461 aaacaatcca gagaaagtgt cactggttct cccgttttct ctaattcttt attgacaaaa
     5521 cttgaacaac gcagtgcagc cggaccagaa ataccgaaat gggtgaaaag taaatccatc
     5581 gtatgttcag tcaatacacg acctttttta tttaaaacgc gtaaagtgat gtcttgtaaa
     5641 gaaagccctt gaagtgtttt ctcctgaata tacggttcat ccgatatcaa gggggactct
     5701 gtcgcataaa gaggcgtcac cgtgtgaccc actcgcttca cgatcttgta cccatctcca
     5761 gtagaaccag tagatggata agtccgtcca ccagttgtta agataatggc aggagcatgg
     5821 aactcttcga attccgtgcg tataccgtaa attttatgtt ctttgtggat caatttctct
     5881 actcgagtag agaatagaag cgtcacatct agttctttca gacgattgag caatgcttcg
     5941 ataatcgttt ttgatttatt tgttacagga aacatccgtc catgatcctc ttcttttaaa
     6001 tgtacgcctt gtgattcaaa gaagttcatg atatcgaaat tattccattg cgcaaacgta
     6061 ctatataaaa actttccatt tccaggaata tgtgcaatca aatcatcaac aggacgattg
     6121 ttcgtaacat tgcaccgacc accacctgtc atcagtagtt tttttcctgc ttttttgttt
     6181 ttttcgatca agagtacgcg tgcgccttgt tcagctgcac tgatcgctgc catcatcccg
     6241 cttgttccag cgccgactac gatcacatca tacagttttt ccattccctc acctcaagga
     6301 aagtgtagca tatctgaaga aagaaaacag aaaaaaatgt tttgagccaa aagagaaaat
     6361 gagctaaaat gagggaagat aagcaaagga gcgtggaacg atgacaaacg caacagaata
     6421 tgttcaagca attcaagaaa agctacatca aaaggatcaa gggcaagaag aatttttaca
     6481 agcaatcgat gaattcatgc ccacggttat gtcttttttg gacactcatc ccgaatatat
     6541 cgaaaagaat atcttagaac ttttaacgga accagaaaga gttattcaat ttcgagtgcc
     6601 ttggcaagat gatgaaggaa actggcgagt aaatagaggg taccgtatcc aatacaactc
     6661 agctatcggc ccttataaag gtggattgcg cttccatcct agtgtcaatc taagtatctt
     6721 gaaatttttg gcttttgaac agattttcaa gaatagttta accggtctgc caatcggtgg
     6781 aggaaaaggc ggaagtgact tcgatcctaa aggaaaatct gataatgaga tcatgcgttt
     6841 ttgtcaaagt ttcatgctgg aattgtcaaa acatatcggc ccatcgattg atgttcctgc
     6901 gggagatatc ggtgtaggag ctagagaaat cggctatttg ttcggcgaat ataaacgatt
     6961 gaaaaaatat gatactggtg tgttgacagg taaacctttg gatttttggg gaagtaagat
     7021 acgtacggaa gctactggtt atggattagt gtattatgtc aaacatctat tgaacgaaga
     7081 aaaagattca tttaatcaaa aaacagtttt tgtttccgga agcgggaatg tagccattta
     7141 tgcaatcgaa aaagtgcaag agctaggcgg gaaagttgtt acatgttcag attctagcgg
     7201 gtacatctat gatccagaag gaatagatgt gaaactattg aaagaagtca aagaaatcaa
     7261 tagacaacgt ttgacggaat acgcaaacca acgtccttct gctatttatc atgctggaga
     7321 atctgtttgg acattgaaag aaaaagcgga tatcgctttg ccatgtgcta ctcaaaatga
     7381 aatagacgaa agcttggccg aaatcttagt agaaaatggg atcaaaatcg tagcagaagg
     7441 cgctaatatg ccatgtacag taaaagctgt ttctgttctt catgaagcag gcgtgtggta
     7501 ttgtccagga aaagctgcta atgctggagg agtagcagta tctgctttag aaatgagtca
     7561 gaacgtccag cgattgactt gggaaagaga gcaagtggat gagcagctgg atcaaatcat
     7621 ggaaacgatt taccatacgt gtcgccaaac agcagccgag tacggcgacg aaaaaaatct
     7681 gcttcttgga gcaaatgttg ctggatttga aaaagttgcc aaagcaatgg cagcagaagg
     7741 tttagtttga aaaatgaaac tttcttggaa atattgtcag cattttgttt atttgcttga
     7801 aaaataataa aaaaaaacgt aaagtattac ctgtagagta aacctataag gaggagaaca
     7861 aatggcacac attcattttg actattcaaa agtagcaccg tttgtcaacg aacaagagtt
     7921 agggtacatg caaagccaag taacagcagc ccacaacgaa ttacgggaag gtactggcgc
     7981 aggaagcgac ttccgcggct ggatcgattt accaacgaac tatgacaaag aagaatttgc
     8041 acgtatcaaa gaagcagcta aaaaaatcca atctgattct gaagtattag ttgttatcgg
     8101 tatcggtggt tcttatttag gcgctcgtgc agcgatcgac ttcttgaatc acacattctt
     8161 caatttatta gatagcaaca caagaaaagc accacaaatc ttctttgccg gaaattcaat
     8221 cagctcaaca tatattgctg acttgatcga agtaatcggt gatcgcgact tttcagtaaa
     8281 cgttatttca aaatctggaa caactactga accagcgatt gcgttccgtg tattcaaaga
     8341 gcttcttgtt aagaaatatg gacaagaaga agcaaacaaa cgtatctacg ctacaacaga
     8401 taaagcaaaa ggtgcagtta aagtagaagc agatgcagaa ggttgggaaa ctttcgtgat
     8461 tcctgatgac atcggcggac gtttctctgt attaactgct gttggtttgt taccaatcgc
     8521 tgttagtggt gcagatatcg atgcattgat gcaaggtgcc gctgatgcaa gcaaagcata
     8581 ctcaagcgac aaactagaag aaaacgaagc ttaccaatac gcagcaatgc gcaacattct
     8641 ttaccgtaaa ggcaaagtaa ctgagttgtt gatcaactat gaaccaggaa tgcaatactt
     8701 ctcagaatgg tggaaacaat tgtatggtga atctgaagga aaagaccaaa aaggcatcta
     8761 tccttcaagc gcaaacttct caactgattt gcactcttta ggacaattca tccaagaagg
     8821 acgtcgcaat atttttgaaa cagttgtgaa ggttgaaaaa cctcgtaaat caatcacaat
     8881 tcctgaacaa gcagaagatt tagacggtct aggctacttg caaggaaaag aagtggactt
     8941 cgtaaataca aaagcatttg aaggaacatt gcttgctcat acagacggtg acgtgcctaa
     9001 cttgctagtc aaaattccag aaatggatgc ttatacattg ggttatacaa tgtacttctt
     9061 tgaaattgct gtaggtattt caggttactt gaatggaatc aacccattcg accaaccagg
     9121 tgtagaagct tacaagaaaa acatgttcgc acttctaggc aaaccaggct ttgaagatct
     9181 tgctaaagaa ttgaacgaac gcctgtaaga taagggtttt tcgctagatt taaataatca
     9241 gtgatagaaa catgttcgct ttaaaatgaa aactggtacc catttaggta ctcaaacata
     9301 ctgcatcgtt tattcagaat aaggggctgt gacaagacgt ttgtcacagt cccttgtttg
     9361 tatagaaatc gattgattgc cttgctcttt agtttgtttt aggactttaa gaacagtgag
     9421 gctgacttcg aaggttaatt ctgttttttt gttttctaga atcagtaagc aaaaaagcac
     9481 tcacttcgag tgcttttttg cgatatattc ttagcttaag ataggcctaa tttttctttt
     9541 acagatgagg gtaccttatc gctggtaatt acttttttct ctatgatccg cttttcgctt
     9601 gctttcaact gcaagatcgt tccttcgggg taagcagaac cattggcaga gtcaccagaa
     9661 gtaatgacat cgaaatctaa ctgtcttttt tcaccgtttt cagcataagc atctacgttg
     9721 tatttaaaac ctcttccaat tacttcccca ttatctgctt tgatatcagt ttctgcaggc
     9781 attggagctt tgactaccgc ataataatct ttacctacat aagtagactg atagtagtcc
     9841 caacctttcc atcctataaa agcaatcaat atgataacga tcaaccctaa aatttttttc
     9901 atgaaagatc tcctttttta tttttatta
//
LOCUS       DAHHZJ010000111         9817 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.112, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000111 DAHHZJ010000000
VERSION     DAHHZJ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.112"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(<1..195)
                     /locus_tag="NBL02_002061"
     CDS             complement(<1..195)
                     /locus_tag="NBL02_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572738.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNY"
     gene            complement(211..825)
                     /locus_tag="NBL02_002062"
     CDS             complement(211..825)
                     /locus_tag="NBL02_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCD2572739.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(972..1655)
                     /locus_tag="NBL02_002063"
     CDS             complement(972..1655)
                     /locus_tag="NBL02_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCD2572740.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1764..2222)
                     /locus_tag="NBL02_002064"
     CDS             complement(1764..2222)
                     /locus_tag="NBL02_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HCD2572741.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2306..4069)
                     /locus_tag="NBL02_002065"
     CDS             complement(2306..4069)
                     /locus_tag="NBL02_002065"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCD2572742.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4290..5750)
                     /locus_tag="NBL02_002066"
     CDS             complement(4290..5750)
                     /locus_tag="NBL02_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572743.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5958..6884)
                     /gene="glsA"
                     /locus_tag="NBL02_002067"
     CDS             complement(5958..6884)
                     /gene="glsA"
                     /locus_tag="NBL02_002067"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCD2572744.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6907..8334)
                     /locus_tag="NBL02_002068"
     CDS             complement(6907..8334)
                     /locus_tag="NBL02_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD2572745.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8365..9579)
                     /locus_tag="NBL02_002069"
     CDS             complement(8365..9579)
                     /locus_tag="NBL02_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HCD2572746.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         2910 a         2041 c         1627 g         3239 t
ORIGIN      
        1 gtaattaaac aatccgctaa tcgtttgatc tggcatttct atttccttac catctaaagt
       61 aacagtagta acgacaaaat ggtcttgcaa tggcggaact tctaagtact gcccttgcag
      121 aaaatcattc actgtttcat attctttttt catttctttt gaatctgtcg atggtttata
      181 gcttattttt aacataacta gtgctccttt ttattcggga cgtgcaatcg tgaaaatttc
      241 tcctattgct gcataatcat ttcctgccaa acggcttaca gcattcaatt ttcttggatc
      301 gatccttcct gtcttatcat catagactgt ttcatttaaa tgatatttct gtacttctag
      361 caaaaataaa tcagctgtta ctttattttt attccgtatc tcgacatgtt catataagga
      421 tgtttcaaaa cgaacttttg cttctgtcaa tgctggtact gaaatgatta ctgaatcagt
      481 taaagtcaag tcagtcaggt acagttcgct ttcctcatta ggcagattgg ctgctgtttt
      541 atttacttct tctacgttgt cctcatcaag gatatggata acagcttctc ttgtctcaag
      601 aatattcttt gctgtgtctt ttactgcatc ttgttttctc tggatcgcca aggataagat
      661 gggtcgattc gaactgacga tattgaagta actgaagggt gcgatattca ctatatcttt
      721 tgctgattta gtcgttacta ctgctactgg tctagggatg actgaaccaa tcagcaactt
      781 gtaattttca cgttctgtca attctgaagg actccgttca aacatggttt cgccttcttt
      841 ctataaaatt cgttaaaaag cagatcttga agacaaaatg aaaagtaatg cttttttcat
      901 cttcttagaa tctgctttaa actatctatc aatttttctt ttttgtcaag aatctgtgcc
      961 gtgcgtgttt tttagttatt tgctttactt gcggcttcga catatctttt agtgatcaat
     1021 tgcggacggg tgataataga tccaactacg actgaaaaag cacctaattc atatgctttc
     1081 tctacttgtt caggagagtc agtatgtcct tccataatta ctgggatatc gacttgttct
     1141 atgatttgat tcaataaatc aaaatcaggt gtctgcgtct ttcgtgaata ttctgtatag
     1201 cctgctaaag tcgtagacaa aagatcaaaa ccatcttctt gcgcttgaag cgcttcctca
     1261 aaagtcgaaa tatctgccat agctaaccga ccgtgttcgt gaatcatttg gacaagttca
     1321 gcagttgttt tttctgtcgg acgtttacga gaagttgcat ccagtgcaat gatttctgct
     1381 tgcgtctcca aaagttcttc cacttctttt tctgtcgcag taataaacac ttctgaccca
     1441 tcgtaatctc ttttgataat ccctattacc ggtaaatcaa cgattcgctt gatttcattg
     1501 atatcttctg tcgtatttgc tcgaatacca actgctccgc cttcttttgc agccaaagcc
     1561 attctcccca taataaaaga actgtgtagc ggctcgtttt ccaaagcttg acaagaaacg
     1621 acgactccgc ctttgatttg ttccagtgta tccacaatat ctcctccagt aatgttatat
     1681 aaaaatacag cttcatataa aaatgtaacc cttttctatt caggagacaa taatgagcta
     1741 tttctttcag cagacctttc ttactaaaag aaaattactt caaattcttc gtatacgacc
     1801 agcttctctt ctgtaaattc tatgttcaac gcttctttat aagcatgttt aaaaaactca
     1861 gcatggactt tccgccaata agctaatgtc cgatcccctt ctccttcttt ccaagcatgc
     1921 tctttcgtga cttcactaaa aggcaagatg gtcacattta tatttttgat gatacaaact
     1981 ggttcatttt ttccattcag gaccacgtct aattgaccaa cttttggtaa aggctcattc
     2041 tcttgtttat acagatcgta accacttgat gtaccagtct ttatcttttt tacaactagt
     2101 tcgctcaact cgtctgccat tttttctgta tccccaaatg aaaagaaacc taatttttct
     2161 gattgataac taggatattt ttcttggaac tttttgacat attcttctac ttgctgatac
     2221 ataacatcca cccctttgct ttttataatg aaaaaaccat ccagatacta gtataaacaa
     2281 ttatctggat ggtgagcata atgaattatt gaatattcaa gacacgcaga ctatcttcaa
     2341 cgatttgttt tggtgttaaa tgataacgtt cataaagctg ccctacagga acgctatccg
     2401 tgaattcttt ctctgcacca aaatttaata cgttcattga agaatcacca taataacgac
     2461 tgatattttc gccaaatcct cctgacaaac taccatcttc taatgtgatg actaattcgt
     2521 gtgtttcttt taatttatct aatgtttcta cgtcattttc tgtgatcgtt cctggattga
     2581 tcaacgttgc ttcgattcct ttttttctaa aagcttttac tgtttctttt cctaattcaa
     2641 gaaaaccgcc taaagctaaa attgctactt ttcctccatt atttactgtt tgataggctg
     2701 gaatactata atcatttgcg atagctgtac catgttctac accatgtgta ggaattcgga
     2761 ttgctacagg ctgcgctgtt tgttcgaacg cccaatctag catagagatc atatcctctt
     2821 ttgttgctgg agcaagatat ttgatgttcg ggatacttga taagtaagga atgtcaaatg
     2881 ttccttggtg tgttcggtca ctagctgaga tcgttccgcc accgactaaa ataatcactg
     2941 gattctcgtt gatcgctaaa tcatgtgata attgatcata tgcccgttgc aagaaagtac
     3001 ttgattggaa gacgactgga cgtactccct gagaagccaa agcagaagcg aaagtgatag
     3061 agtgctgttc agcgatcccc acgtcaaaat aatgatctgg atgtttttct tggaatgttt
     3121 tcagtccaaa ggcgcctggt atcgctgcat tgactgcggc aataggagct gtacttgttt
     3181 gcattttctt atccaaataa tccatgatga tcatattaaa tgtttctcca gaagttactt
     3241 gctttgcctc cccggtggac agatcaaatg gtacatgcca atgatatttc attttattat
     3301 cgattgccgg ctgataaccg cgaccttttt ccgtatggac gtgaagtaca atcggatgat
     3361 caatatcttt tacttcttca aataaacgga tcaccgtttc cagatcgttt ccattatcca
     3421 cgtaacgata atcaaatccc attgctttaa ataggttatt gctgctttta ccgttggttt
     3481 gacgaagctc agctaagttt ttatataaac tgccatgatt ctcatcaata gacatctcat
     3541 tatcgtttac taatacgatt agattggatt gtaaaacacc accattgttc aatccttcaa
     3601 aagctaagcc accacttagg gagccgtcac cgataaatgc taccacattt cctgattctt
     3661 tcttcagatc tctcgcttta gctaaacctg ttgctaagct gatagatgta gatgtgtgcc
     3721 ccactgtaaa atagtcatgt ttactttcat gttggttgga atatccgctc acttcacggt
     3781 aatgtccttt ttcaaaacca ttttttcgac cagttaaaag cttatgacca taagactgat
     3841 gggaaacatc ccaaacgatt ttatcctttg gtgaatcaaa tacataatga aacgcaatcg
     3901 ttaattctac tacacctaga ttcggaccaa cgtgcccacc tacatcgctg tccttttgca
     3961 agattttttg gcgcatctct tctgccaact gtttcatttc ctcaacagat aattttttga
     4021 catctgcggg gctattgatt gcatccaata catgtaatac tgtactcata atacattctc
     4081 tcctccgtac tatcttcttt taatgaaaat agatttgtta cactttatct attctactac
     4141 ttagagttga ctctaagtca atagcatttt aaaaaaattt cagagaaagc ttgttatata
     4201 agtagatctt cgcacgaatc aggactcaaa taaaaacctt ctacagacaa ttaaaaagct
     4261 ttgtagaagg tctcctaatt aaggtctcat caagaaatat gtttttttaa taggtgaagt
     4321 tcctgagcaa tatgatacaa atggatgttt gcacctttta atgagattgg atagatcttt
     4381 gtcttttcag tttctaatcc attttgatag ctagcagcat cgcacaaaac cactcttttt
     4441 ttcgcacata gacattcatc aggcaattct tctaatgaac gaaataaaat aggatttatt
     4501 tcagcgatgc cttcttccgc atacaaatga aattgggttc gataaaaaaa ttgtttgact
     4561 gaactttctg aaacaacaaa aatttctatt tcctgtctcc ccgcttgttc actgttgatc
     4621 aaagaaagac ttttgacgaa ttttcgaatg aaattttcat tccatcttag ttggatttga
     4681 tttttctttt tccactcttc caatatttct acaatttttc cgtacagaag ttgttctttt
     4741 tctgatagaa gatagatcct ttccggtaaa aatacttgtc catctgccca caacgtacgg
     4801 acaaattgta aaaaggattg cttgaaaata aaattttctg catcgtcgat ttcagcgata
     4861 ctaataatct gtaaaatcaa ttcattcagt gataggtcat tgagaacaaa atttttataa
     4921 acaacttcga aatcttttct taacagctcc atgttctcat tggtataatt tctactgttg
     4981 aacaaagaaa aaatataata gacatcttct tcttgtaagt ctagaattcc tttttgctct
     5041 tccaatcctt gacagactaa ttgaaataac ggcttcgttt ctgcgctttt cttatcttct
     5101 tctttcagtc caaggggaaa tgcctttctt ccgattgcga tctctattcc tcgtatgata
     5161 aaggactttt catcttccga atatcttctt ttaaaaaaat gagtctctac ataatcaatc
     5221 aactcgttac aagcttcttc tacatacgga tttttatttt catagccttc ccaattgaca
     5281 taacgtacta aagctagcaa gatgttccgc gagtcaaatt ctgggataat gatttcttta
     5341 tttttcaaat aatcattttc tttaaaaaaa tccaatacga tttttttgat tgtatatact
     5401 ttgctcaaag aaataaattc aagatcagcc agttctgtac tcgtgaatat accattaaaa
     5461 taataactta aaaactttaa aaaataagat tgaccgtaca gaaactgtgt gagctcagaa
     5521 agagcaatac catgtttgaa tacgagttta tacttatgct tctctcttac ttgttctttt
     5581 atacaatatt ctagacactc ggctagactt tctagatcgt gaaggatcgt tggtgtagtc
     5641 acccttagag aaatagctgt ttgttcgatg tctatttctt ggaattcaaa taattgttca
     5701 cacagataca cttttctaaa tatatttcgc tcgatgtaat tttccagcat tcctgataca
     5761 cgcctccagt ttttcttatg attttattta atcatatctg taaatcgtat ttttgtttcc
     5821 tttttattta gaaaagccct ttcaaaaacc aataaaaaaa gaaactggga caaaagtcaa
     5881 acaagctcac aatttcagcc tcattgtagg tagctttgag ccgtttggac ttttacccca
     5941 gcctctttca agaatggtta ttcaaagatg tcagcgtcta gttctttgac tacatctttc
     6001 aacaattgga taccagctgc gctgttacca aatggatcta gtgccgggct gaagacacct
     6061 atgccgtaac gatttggcac agctgccatc aacccgccgc ctacaccgct ttttgcaggt
     6121 acaccaacat ggacggaaaa ctctcctgat tcatcataca aaccagcagt tgtcataatt
     6181 gatttgacga ttgtagcact ttcttcagta atgatttgtt tgccattcca aggagcaatt
     6241 cctttatttg ccagcacagc agctaatttt gctatgcctg tagcggtgac attgagggag
     6301 cattgtctga aataagcatc gagtatctcc ggtacgtctc cttcgatcat ttgattacct
     6361 ttcatatagt aagcaagaga gcgattgata tcacctgttc ttgattcaga atgatagatt
     6421 tcttcattca atgtgatatt aggatcatca cagatctctt tcataaatga caagattcgc
     6481 tcaaattttt catctgcatc tttaccggca atcaacgagg ttgttgcaat cgctcctgca
     6541 ttaacaaaag gattcattgg atgtttgcga tgattgattt ccatattcat gatagaattg
     6601 aaggcaaaac cggttggttc cgtgttgatt gtttgaaaga ctttttctat tccgttgtct
     6661 ttgattgcta ataacaatac gggaactttt gacatgcttt cgatggcaaa gcgcacatcg
     6721 gcgtctcctc cctcaatcag ttgttttgta tccaaatcat aaatcgccat acctaattgt
     6781 ttgggatcca cttcagctaa tgctggaatg taatccgcca cttgtcctgt ttgataatag
     6841 tggaaatttt ttccaatcaa ttgttctaat tcttttactt ccatttgaat aaccagcttt
     6901 cattttttat ttttctgcat gctggaattt atgacggcat gcgtagatcg taaatggaat
     6961 aactaagacg ataagataag aaacaatcag aatcgtttca taaatcccat gctgtccagc
     7021 tgcgatacta gcaggtggaa caaatgagat acaaaaagca aaaagtgaag tcaaaagacc
     7081 taaacaagca acgattactt ttacaacttt tccgcctgga acctgatagg ctctttcaag
     7141 actgtcatct ttaaaaacca gcttcaaata gcccgcgaaa aacataaagt acaacatcag
     7201 gtagattaca acagtcaatg acatcgctgc aagaaatgaa agattattcc ccccgccgct
     7261 taatgtaaag acggctgccc agattgttgc aacgatccca tttgcgatca tcaaatgagc
     7321 aggaacaccg tgtgtgtttt cttttgcaaa atattcagga agtaatcctt cttctgctgc
     7381 tacgtgaagt gcttttgaag gagaggttac ccaagaagca atttctccga tgacacctaa
     7441 acaaacaaga atcgcaagaa ttttcgcaat ccatgcaaga tttggatcaa tgtgtcttaa
     7501 taggaattct aacgattgaa tcacccctgt attaagagac aaaccatgtt gaggtactgt
     7561 agtagcgacg gtcaatccgc caaaagtatc taaaatgatt gccagaataa ccaatacaaa
     7621 cattgctaat ggataatcct ttttagggtt ttccatttca tttgcatgac tggcagatgt
     7681 ttctacgccc atatatgcta aaataaagga aacaaaaaca accagcgtag gcaatttcga
     7741 aaaatcagga atcaatgttt tcatagacag tatcgtttgt agtggagcgc ctgaagcgac
     7801 ataatggatc cctaagccaa acaagactaa tgctggcaac aagataccag ctacaaaacc
     7861 gatccgagca atcaatgaag tgtatttggt tccttttaac tgtgagagta aaatagccca
     7921 gaaaataatc aataggattg ccaatttcag atatttattg ttttgtatag caccaatacc
     7981 cgttgcatag gataatgcac cagttaagaa atacagcatg gtcaagtagc caactgttat
     8041 ctcaaaccat tggaaaaaga ttgcggcaaa accgaatcgt tttcccaatg tgcgactgac
     8101 ccaagtatag acaccgcctt tttcccatcc tttcaccgtt gccatctctg ctgcgcacaa
     8161 tgccacaggg ataaaccata gcaaaccgcc taataacaag aaaaatacta agctgaagcc
     8221 tgaggtcgcg aatgtgggat attcatataa tgaaacgacc attgagatgg tcatagccaa
     8281 aaagccaaag aacgttaatt tttttgcagg aattttcgtt ttgctttgtt gcatgaatct
     8341 tcctcttttc tatttgaatt tgtttcaatt gagtttgttg ccaaagaaga aaaagtccaa
     8401 tccttcttct tgttcttctt ttgtagtagt gatcgttgta aagtgcatcg ccgattttaa
     8461 aatcactgtt gtcagcacat aggcatacac ggcagtaatg atgacaccaa tcacttgaat
     8521 ccctaagaag taccagctgc ttggattcgt tagtaaatgg ctgttagatg caaagatccc
     8581 tatcagaatc gtacctgtga atccgcccat gccatgaaca ccccacacat caagtgcatc
     8641 atcccattgt ttctttttac gaaaatcaac agcagcatga cagacgatac ccgcaatgat
     8701 cccaatcagc atggcacttt ttgcttctac ataaccggca caaggcgtaa tggctgccaa
     8761 tccagcaacg gatcctgtta gtacgtcgac aaaatcaaag ccatttcctt ttacttttgc
     8821 aatgatcagc caagtaatca tcgcaaatgc aagagcgatg aatgttgaac caaatgcatt
     8881 gactgcttgt tcatctgctc gaagtgcacc accggaatta aaaccgaacc agccaaacca
     8941 aagcagtcct gtaccgatag cagctgccat aagactgcta tgctgacttc tttctcctaa
     9001 aggaatcttt ctttgaccaa gtgtgaggat acatgctaaa gagccgaatc cagctgtcgt
     9061 gtggatgact gctccaccag cgaaatctct aaatcccatc tgatccaaaa atccgccacc
     9121 ccatatccag tggcaaacag ggaaataaat cagcagattc caaaaaataa caagtataat
     9181 atagcctttt aaattcaatc tccctgcaaa agctcctgtc atcaaaggaa cggtaatcac
     9241 gataaacatc agttggtaaa gaaagaacat taaaaatgga atcgttgcac catgaaacat
     9301 gttggggaag cttgtaacat gtctcatcaa aaagaaatct gccgggtttc caataacacc
     9361 gccgatatcc cgtccaaagg ccaatccgaa tccaccaaat acccacataa tcgttgttat
     9421 cccaacggag agaaaagatt gagacatgat cgttagtata ttattcttcc gtgctaatcc
     9481 gccgtaaaaa aaagctagac caggagtcat caggcaaacc attgctgtac ataaaatcat
     9541 gaatgctgta tctcctgtat tgataggtaa tgtattcatt tttttcgcct cctttctacg
     9601 attacgagta taaaagaagg tgtagttgtt tttttcttct gtttttttga attaatactt
     9661 gcatttataa aagacagaca attaataaaa gcttgatgtg ttgtgctctt tttcgttttt
     9721 cacacaaata aagacaaaaa ataaacggtc ttctttttat agaaaaccgt ttattttcgt
     9781 ctttttttat ttttaaagat attttttcat aaggaat
//
LOCUS       DAHHZJ010000112         9706 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.113, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000112 DAHHZJ010000000
VERSION     DAHHZJ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.113"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            17..496
                     /locus_tag="NBL02_002070"
     CDS             17..496
                     /locus_tag="NBL02_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572747.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            529..2757
                     /locus_tag="NBL02_002071"
     CDS             529..2757
                     /locus_tag="NBL02_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCD2572748.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            2886..4529
                     /locus_tag="NBL02_002072"
     CDS             2886..4529
                     /locus_tag="NBL02_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCD2572749.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4602..5147)
                     /locus_tag="NBL02_002073"
     CDS             complement(4602..5147)
                     /locus_tag="NBL02_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HCD2572750.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5170..5655)
                     /locus_tag="NBL02_002074"
     CDS             complement(5170..5655)
                     /locus_tag="NBL02_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCD2572751.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5645..6457)
                     /locus_tag="NBL02_002075"
     CDS             complement(5645..6457)
                     /locus_tag="NBL02_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HCD2572752.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6596..8128)
                     /locus_tag="NBL02_002076"
     CDS             complement(6596..8128)
                     /locus_tag="NBL02_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HCD2572753.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8237..9268)
                     /locus_tag="NBL02_002077"
     CDS             complement(8237..9268)
                     /locus_tag="NBL02_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCD2572754.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3227 a         1803 c         1882 g         2794 t
ORIGIN      
        1 atgaggagtt aaaataatgg aaataccaac aaatttaaaa cataaaccag taatcgttgt
       61 agaagactat gataaagttg atggaagaaa tgcattaaat atggatgcaa aagggctttc
      121 actgggcctt gctcagtgga atgatcgtgg aaaagtcgat atctcaggca aggtatggcg
      181 acatactgga gaaaaatggt cacgacagtc ggaagaatta ccaatcacac gtattttaga
      241 tttagcgatc ttagctgcgc aaggaagttt atacttccaa gatgcttatc gttatgaaaa
      301 attttatgat ccagaaaatc cagtcgtaga catcattgga ttacaaggga accgaatgac
      361 ggtagaagta gataccaaaa atccaaagat cgatgaagac atcattttgt attacgatac
      421 actacaaaaa gacggagaac tgattgggga acgattccgt attttaaaac gtcttttaga
      481 tgagctagga tattaataga cagagatgat caaaagggga agagaagcat ggagaatttc
      541 tcactaacag aaaaccgaac cttttcacgg ctagaaaagc aaatgatctg gaagaaaccg
      601 aaaagccatc ggacaagtgc agaagaatgg cggatcgcct ccgaaatcaa agggaattgg
      661 caagatcctg aaatgaaaat cagtaacgta cttttggaag gagatgccgg gtcggggaag
      721 acacaattag ccaaagcact atctgctgat ctgcaacttc cctatacaaa agtgacctgt
      781 tttgcagata tggacaaatc agatgttttc ggagccttat tacctgtagt agattctaat
      841 aaggaacaag accaagaatt actagaagct atttaccaaa cagatacact tgaagctgta
      901 ctgacactga tccagaacca ttacggcatt gatccttttt ctgctaaaga aaaattagca
      961 gagctagttc aaaggatcga agctgattat ccagatgcaa tccattacag ctattaccct
     1021 tctgaaatcg ttcgagcgat tgaaaaaggc tatttattag agattcaaga gccgactgtc
     1081 atccgagatg cctctgttct tgtggcactt aactcagcgc tcgaaccaaa tgggatgttg
     1141 aatttaccga caggaatcat tcgtcgccat ccagattgcg tcattgtgat cactacaaat
     1201 cgaaattacc aaggaaatcg cccattaaat gaatctttaa gagatcggat gcaacatgcg
     1261 gaaaaaatgg atcttcctgc cttagaagtc atggcagaac gtgcgatggc gaaaactatg
     1321 atccaaaaac aagaacttct cttaaagatg gcagagatta tccgtttgtt ggatgaaact
     1381 gcaaaagcta acgccatcaa aggtgtagca ggaatgcgtt cttattttta ttgggcaaat
     1441 acctataagc aaggacaaga tttactccag tcgatttatc caaaagtttt gtataaatta
     1501 tccactgatt cagatgaatt ggcaattctt gaacaagctt tgtctgacag tggattgcta
     1561 ctggaacttc aggacatatt gagaaaagaa aaatggggtc atcctcaatc tgagcataca
     1621 aaagaaagaa ccattagcgc cgaagaagca aatgaacgaa acatcactga aaatgctgaa
     1681 gaaattctgc gcttagatga aaagaaatcg ttgtctgctg aagaaccaaa tgagggagaa
     1741 gagaaacaag aagaaacaat agaaaaaaac gagattcctc ctcaaatgca aaaagaacgc
     1801 agtagcgata tcgaaggaaa aaatcaaaaa gaacaacaaa caggagatga ttctcaagaa
     1861 atgtctgctt ctgacatgga agcacatgat aaggaaatca agaagcaact gaataaagaa
     1921 gcgcgacaaa tcatgaagga aacgattcat gaaaaagaag gtctcatcgt tcaccgacca
     1981 gctttccaag gaaaaacgcc ggaagcacgt gagctacaca tgcaagtaat gccaatagta
     2041 gaatcactta gccggcaaat tttagagtta ctggataatg aacaaacaga gacgtaccaa
     2101 aaaggaaaat acgaaggtca gcggttcaat gcaagcaaag tagcatatgg tgatttgaga
     2161 aattttgaca aaaagaaccc gccacatgag caaccttctt tagcggtagc tgtacggatc
     2221 gatgaatctg ggtcaatgat acgggatgat cggatcgaag cggcaaaaaa agcagcgatt
     2281 gccattgccg aattttctaa gaaagtcgat atcccattgt tgatttacgg agatacagca
     2341 gaccgttcgt caagagaaaa gacttctttg ttttcttata aagaatttga agacggattt
     2401 caatggttaa atgaaaaatt agtcactatg aaaccaaggc aaaacaatcg tgatggtgct
     2461 gcacttcgtt tagcagcaga aaaattaaac agacaatccg caacaaccaa actgttgctt
     2521 aatattagtg acggtcagcc aaaagcgttg ccggattata caggcaaaaa agcaaaagaa
     2581 gatatccagt ctgttttgaa agaatatgaa cgtcaaggaa tcctatttgt ctccgcagct
     2641 attggccaag acaaagaaga aatcaagtcg atctacggtg aaagtcgttt tgtagatatt
     2701 actgacttga atgaatttcc caagcaaatg attcaactga ttgcccgcta tctatgataa
     2761 aataaagctc agcgaacgca cttagcagat tcgttgagct tttcgtttct agttaccggt
     2821 aacaaggata cttctaaaat agaatcggtt ataatcaaga gggaccgtta aaggagtgaa
     2881 acgatatgat acaagaacaa aaatggtgga aagaagcagt aggctatcaa atctatcctg
     2941 ctagtttcaa agatagcaat ggcgatggtc aaggagacat caatgggatc cgtgaaaaac
     3001 ttaattattt gaaagatctt ggtatcggct ttatctggat caatccgatc tatcaatctc
     3061 catttgtcga taatggttat gacgtaagtg actatcaaga tatagaagag agattcggaa
     3121 cgatggaaga ttttgattta ttgctaaaag aagcacatga attagggatc aaagtgatta
     3181 tggatcttgt catcaaccat agttcggatc agcatcaatg gtttgaagaa tcgaaaaaat
     3241 ctaaagaaag cccgtatcgc gattattaca tttgggtcga cggggtagat ggcaaagaac
     3301 cgaataattg gacatcgatt ttcggcggtt ctgcttggga gtattctcat gaaactggac
     3361 agtattatct gcatgttttt gcaaaagaac aaccagactt aaattgggaa agcgagaaat
     3421 tgaaagaaga actattcaat atgatccgat ggtggttgga taaagggata gatggatttc
     3481 gtttagatgc gatttctcat gtaaaaaaag atgaatattc agtaaaagcc acagagaacc
     3541 ctttctcacc ctttcaaaat gtctcaggaa tcgaagaaca tttgaccgat ttgaaacatg
     3601 tttttgaaga gtacgatatc atgactgtcg gagaagcaag tggtgtcact gcagaagaag
     3661 gaccacaatg ggtagggaaa gacggttatt tcgatatgat attcgaattt gatcatatcc
     3721 atatctggca gcaagaaaaa gaagggcaat tagatgtcct taaattgaaa catgcattaa
     3781 gcgcttggca aacttcattg gatggtatcg gatggaacgc tttatatatg gaaaaccatg
     3841 atgttcctcg tgccgtttca gtgtttggag atactcgtcc cgatttttgg gctatgtctg
     3901 caaaagcaat cgctatgatg tatttctttt tgcaaggtac gccatttatt tatcaaggac
     3961 aggaaatcgg aatgacgaat atgccctttg aatcgatcga tcaagttgat gcagtagatt
     4021 cgaaaagact ttataaaagg ctgcttgcgg aaggtaagac aagagaagaa gcattggata
     4081 tcattcgtga aacaacgaga gacaacagca ggacacccat gcaatggact tcggaacaat
     4141 atgctggttt ttctacacat gaaccttggc tgatcactaa tccaaatacc aaaacgatca
     4201 atgtagaaca acaagaatat gaaccagagt cggtcctgca attctataag aacatgatcc
     4261 gcattagaca aaccaataaa ggtctgatat acggttctta taaagaatat cttcatgaac
     4321 atccacaact ttatgtctat gaaagatatt tagaagatga acaatatctt attatggtaa
     4381 acttaacaga atcacttgct gactatgaac ttccaaaaga agcagatcag tcctggacat
     4441 tactcttgtc aaattcctct agcggagaat ttgaagctaa aggcatacta gcaccttatg
     4501 aagctcggtt atataaaaca aacaagtaaa acttcctcgt taaagttcat tagttggact
     4561 ttgcgaggaa gtttttcaga ttgctctgtc aattactcat ttcaataatt cgctctagga
     4621 ccaccgatat atttaggacg agagcgaagg gctgtcacgt gagcttgtcc aagggttttc
     4681 aaacgaggtc tgacaggtac ttggacatgc ttgatatgca taccgattga tgtgtcaccg
     4741 atatcgatac cggcttgtcc aactacgtgt tccacttcga caggatcatt gaactggtca
     4801 aaagcagcaa cagaacaagc acctccagca tgtagtgcag gaacaacaga gacaatttcg
     4861 aaattttttg cttctgccac tgttcgttct actactaatg ctcggttcaa atgttcacaa
     4921 ccttggacag ctagcgcaat ttctttagga tccaacaact cttttaacgt acggatgatc
     4981 cattgaccaa cttccgcact ggaatttttt ccaatatgtc ctccaacgac ttcgcttgtg
     5041 ctgcatccta aaacaaaaag atcacctttt ttcaagtgtg cctcagccaa tatgtcatta
     5101 actatttcag tcaattggtc ttttaattct ttttctgaca acttcatttt ctaaaacctt
     5161 ctttttcaat taatattgct tccgtgcttg aatacgttga agacttgcag ttataggaaa
     5221 ggcaagtccc attccaatca gattttggaa gatgtttcct ggtatagaag ccactccagc
     5281 cggccaatca tataaaatcc aaccggcgat accatagccg atgatcataa ctaagcttcc
     5341 taataaaagc cctaatcccc acatacgagt agaagatttg ctgaaagaac cagcaacata
     5401 tccttgtaac ccgtggatca ctaatgaaaa aactgcccac tgcggatatc ctgaaatcaa
     5461 atcgatcaat agaccactgg aagcaccaac aactaatcca cccgttggac cataaagaaa
     5521 agcagcggta tagatcccgg cctcgcataa tgtcacaaat ccattcgtag ccggtacagg
     5581 gatcaaaatt gttaatgaaa gggcaactgt caacgcaatc agcatagcat agatcgttaa
     5641 ttgcttagtt ttcatttctc attcctcctt tttgaatacc gccagaccag acacttccgg
     5701 tatgatcgtt catcagcacg ccattagaaa tacaatgggc aacaaatttt ttactttctg
     5761 ctactgcttt ggtaaaatct gcccctaaca caagattcgc tgttattgca gaagaaaagg
     5821 cacaaccggc cccgtttact gttgtagtgt cgattttttt atgagaaaat acctcataat
     5881 gatcaattga atagagaaga tccacagctt catctccagc catccttgtg ccgcctttga
     5941 tcacaatgtt ttttgttcct gaagcagtga gttctttagc gagatggatc atttctgtta
     6001 acgaagaggg aagagcttga ccagaaagta aagcagcttc tgccaaatta ggcgtaatca
     6061 tcgtaacaag tggaaaaagt tcttcgatga gctgcgaaat atacaattga ttgatcacag
     6121 cttcagtttc tttgaatgcc aaaacagggt ctaaaatgat tgggatattt tgttttgttt
     6181 ttatgaagtc tcgaacaacc atgattgctt cacttgaatt caataaccca attttgatac
     6241 catctaatga agtcatttta tcaatcgaaa ctaactgtcg tttaagcaat tccaaagata
     6301 tagattccag cataaatcgc tcattaatat ctagtgcccc tatagaagtc aaggcgctta
     6361 gtccgaatag accaaaattc tcaaaagttt ttaaatctgt ttggattcct ccaccagcaa
     6421 atggatcaga accgccaatt gtcaagattc ttttcataac attttcctcc atgaataaga
     6481 gtataacgaa attttcaaaa aagaaaaaca gccaattggc atatttttat ctcatccaat
     6541 ttaaaaaaac aaaaagaaaa gaaagaccaa aatggatctt tctttcctta aatagttagg
     6601 acttttgtga ttttaattct ttctcgattt gaagccgttg cactttcatt gtagctgttc
     6661 gcgggattgc atcaaaatca cggatgatag ggacgttcaa gcgaggaagt tcatggactt
     6721 gctcccacca tcgattccaa tccatttctt tatcaggagc taaagcaata atcggttgtg
     6781 gagaatgatt tttatccctt acaatcacta cttctgccaa aaaatctaac gaatctaata
     6841 aaaagtcttc aatagctaat gtactattga tcgtttcgat caaatccact tgccgatctt
     6901 tcaaaaatag gtggccatgc ttatccataa aaccgtaatc tccgctatcc caccaatcgc
     6961 cataaacatt ttcctggaac cgtgcatctt ccttgtaata agtcaaagcc cgtcctttag
     7021 acagtaattg tatatgacca tctgtgtttt caggtaggac attcccaact gaatctgtaa
     7081 tccgagcgct tgtcatgtct tccagtccaa cgcccatatc tcttgcatca cttgttttca
     7141 aactctctag tgtatgagcc ttaagaatca ttggtccgca ttcactttgg ccatatactt
     7201 gaagaaatac cgcatcctgt gccatcgacg ttcgcaaaaa tgtcttcatt gtctggttgt
     7261 ttatagcatc aaaagtggag tgatagtatc ggatacccga aaaagcttct ggatgctcct
     7321 tagcagtaaa acgccactga acaaagttat tcggatgggt ctctagtgca attggccggt
     7381 gagcttccaa catatgaaca acttttgaac tttgtgcgtt tgctaaaggc atcataggaa
     7441 atcccatgga catcaaagaa gagataccaa tattgaatct ggaatgcact ggagagatgt
     7501 gaaaagcgat taatttcttt tccgaaattt ttgtgaaaac tgttttttgc catttcgtcc
     7561 gccatcccat agaatgagca gaatgacaaa taagtttagg gataccagtt gtaccagaag
     7621 tatgggtcat ataagaaatt tcatttttgt ctaaaaatga ttgagaaaca gacaaagcag
     7681 gttgttccat taaataaaga ataggaatct gttttgattt tgttccattt gaaatttctc
     7741 tcactcgctt ttttgtctca tcatcaaata aaatgaaagg gtcttctaaa cgatcgataa
     7801 atacttccat tgttgttgtt ggtagatgat aagaagtcat gacaggcact gcaccaaggt
     7861 aagacacaga aacagctaga aggtatgtgt caaaagcgga actcttataa ataataactt
     7921 tgtctccgga tttcactcca agatgagcca attgataagc ccgtttcaac acactttcgc
     7981 agcatttttt gtaagttgtt tcaagtccta attctgggaa agctgaaagt ggctcatcaa
     8041 agataatcgg ggtctcggaa aagttagctg cagcttcttg aaaattcgta aataagttca
     8101 aaggggaata cgttatggtc tccatcattc acactccatt atcttttgtt aagtaatata
     8161 caaattcaag ataatcgtaa cataatcctt tatataataa catagtttat tgtgaaaaaa
     8221 agatcataca tttgttttat atggaagaaa cccgaacgaa tgccgctaat tcgtgataag
     8281 aaaaatcaac catcttttcc cagatatctt gaaaagaatg ggatgagagg tatcgattac
     8341 cacataaata atttaccgcg tcagcatcat ctaaatatag ttctgcataa taacgatatg
     8401 tttttctgca tagttctggc gatacgtttt tatatactgt aattttgctc aaaatttggc
     8461 gaatagtccg aggagaaatt tgctgatcat gtgaggaacg aaaaacaaaa gaattttcgt
     8521 ccgtattttg tgctgttaac ttaaaatagt tctccagaaa tggcatcgct tcttttggta
     8581 ttggtacttt attcagtctg ttttcttttc tattcagatt aagaatctgc ttggtcaaag
     8641 atacatcttt acgtttaagt tctactactt catggatctt taatccgcaa tacagaagaa
     8701 tggcagtaat tgcaaggttc ctcggtctat tcttttcttc aaagctagct tctctgcttg
     8761 tttgaaagcc ttcaatcggg ataaacaaat gctcaaaatc tttgaaaagt tctgcatcga
     8821 tatatcgcgg agctgctttg gttgtttccg gtaaacttgt acccaaaaga gaaatctctt
     8881 cttcaatcag tcctttttga tacaaaaatc gtaaaaaatg atttaatgaa tattgcttcc
     8941 ttgaaatccc gtaatagcta ttttcaaaca attgctccgt ttcttggttc gctgtagttt
     9001 ggaacgaacg agtatgtcga tagattttag ctaagaaatt ctgataatct tgtttttcta
     9061 aatggataat cagctgatca tccacttctt cagaaaatac ttttctcaaa taatcagtaa
     9121 acagatgaaa atcttttgcg tattcatagc gactgcctaa tgaaagggaa gaacgatttt
     9181 cttctaaaaa ctgttgaaag acggaaggga gcaagtgtgt acgttgcttt aacttttctt
     9241 gcagttgttt tctagttttg ctgtccatta caaattccct ccaatagtgt tcattttcta
     9301 tattttaccg catttcatca aatgaagata gacagtttgt cttgatctta ggaagcaaat
     9361 ttttaactaa gtactagcta aaagatatga agtaagaaga tacattttta gttattttat
     9421 tagtgcagtc attcttttga tcttttaatt gatttatcct ctttttagct atgtggttac
     9481 ttcatgagca atgaaatgga tttggttcgt tctaatggca ggaggctctt tcttttattt
     9541 ccgaaaaatt agaaaacatt ttttcagaat cgattggctc tccatttatt caataatggt
     9601 ttgtctaacg atattgttgg gataattctt aaagaagtat tagaaatagt tggtacttca
     9661 ttactttaca tgaagattta ttctatggta ggagcaatta tattga
//
LOCUS       DAHHZJ010000113         9538 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.114, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000113 DAHHZJ010000000
VERSION     DAHHZJ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.114"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            3..608
                     /locus_tag="NBL02_002078"
     CDS             3..608
                     /locus_tag="NBL02_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCD2572755.1"
                     /translation="MAKTTTVTTFDNISIKRISFNFKNATNAISTDCNGQLDGETEMQ
                     RIVKKCGSTEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDERIKSGIYSYGPDSVG
                     EDFSLAAEIVDDFEEKNKLIGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFGK
                     FYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            628..990
                     /locus_tag="NBL02_002079"
     CDS             628..990
                     /locus_tag="NBL02_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572756.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            993..1175
                     /locus_tag="NBL02_002080"
     CDS             993..1175
                     /locus_tag="NBL02_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572757.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            1192..4611
                     /locus_tag="NBL02_002081"
     CDS             1192..4611
                     /locus_tag="NBL02_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCD2572758.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITVIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWVSIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSIFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            4662..5399
                     /locus_tag="NBL02_002082"
     CDS             4662..5399
                     /locus_tag="NBL02_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCD2572759.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVVGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINYFNCTGLSKIQIYRNGLR"
     gene            5409..7700
                     /locus_tag="NBL02_002083"
     CDS             5409..7700
                     /locus_tag="NBL02_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCD2572760.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITASQAWQNAKTLAKPVIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            7724..>9538
                     /locus_tag="NBL02_002084"
     CDS             7724..>9538
                     /locus_tag="NBL02_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572761.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGGYDYSGNPNLMANINADSFS
                     QGSGALSVVDDGDEVVVTLDPNNKLEIFKAKSQPALVVGKTYTMSVEIMLEGDFTGDP
                     SKIGLRYIKMPNWVSGLYTRNTLTATKGVWQKLTGTVKITAASDNAESWFIMLQNKDA
                     NNSLSGKLRLRHAKLEEGSTATPYQPNLLDAPYYLSKVALGENIADPAVSFPIKTSAY
                     RLYGVNMLEEFKVGQRYTITIKGTKPATQDFWAYNGGNISLERMTPVEGLVDVWTCSF
                     TILKLDSSSPSLLSIYQTPQSTVGSCQIDWLKIEKGDTRTPNIEQYKYRGIGMRDSNN
                     PKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKD
                     AQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTG"
BASE COUNT         3342 a         1466 c         2032 g         2698 t
ORIGIN      
        1 caatggctaa aacaacaaca gtaacaactt ttgacaatat aagtattaaa agaatttctt
       61 ttaattttaa gaatgcaaca aatgcaattt caactgattg taatggacaa ttagatggcg
      121 aaacagaaat gcagagaatt gtaaaaaaat gcggttcaac agaagtaaaa tcgaaatcta
      181 aaccaatcaa tatgacggta acaattactg cacatgtacc gatggaagtt tatcgacgct
      241 tcaatgggtt gaaacaagat gaacgtatta aatcgggaat ttactcttat ggtcctgatt
      301 ccgtagggga agatttctct cttgctgcag agatcgtgga tgatttcgaa gaaaaaaata
      361 agctgattgg tatgttagca tgcacttcga atacaggatt aacattctct attgaaaatg
      421 gtgcggatga agtagctgcg ttagaactag aaacaaaagt tatgcaagat gaatttggta
      481 aattttatca tgaagcaatt gttgcagaac ttgaagaaga cttaacagat caatggatga
      541 cgaatctatc tgctgatgtg attaaaaaaa cttcagttgt gacaactacg gccactcaat
      601 cacagtaaaa aaaacggagg tagcgaaatg aacgaagatt actcaaaaat tgaactaaac
      661 gatggaacaa ttttgaattt agaacctaaa ctgaatatca agaaattatt gatgatcaat
      721 agagatttta acacagacga gtttgcaaaa atgactgtgg gaaaaggatc catggatatt
      781 tctgttattc aaggtgcaaa ggctgtgtat attgcttacc gccaagcgaa catgactgat
      841 tatatttcat tcgatgaatt tatcgataaa tgggattttg atatggctac tgccagctat
      901 atttatcaat tgatgatgtt caaacaagca cgcgatgctt atcaaaaaga atttgaaaaa
      961 gcaaataagg aaaaaaagct tcaaaagtaa aaatgccaaa gctcttagtt gaaacgtggg
     1021 tcgatgtcta ttcgatgttg accgacgttt tttctatgcc ttcagatttg gttttaagcg
     1081 atatctgttt agatgacatt ttgcaaatgg cttacaacaa gagtgcttat gaaggatgga
     1141 aaaactatgc aataaaccaa tcccagaaaa actaaagaaa ggaggtaaaa aatggctaaa
     1201 aagagaacag aagcagaagt aacattcata gctaacgatg acggattgaa atctacgtta
     1261 aaagaaatca gtgctgaatt aactaaaaat agagcagaat taaaactaga acaagctcaa
     1321 ttacaacaga ctggttctga atcagacaag ttaggaagta aattatcttc tttagaaaag
     1381 cagtatgaat tacaaagtca aaaagttgaa gtaactagcc aacgtttagc caatgccaaa
     1441 aaatattatg gagaaaattc caccgaagtt cagaaacttg agagagaact gattaaccaa
     1501 caaacagcgc aacaacgttt gtcaaacgaa attgataaaa cgagtaatgc actagctcaa
     1561 gcaaaaggcg aaatacagac gtacgagtct acaatgcaac agttggatag tgaacaaaaa
     1621 aatgttcaag ctagtgcttc tctgattgaa tccgaataca aaaaatggca agcaactgct
     1681 ggtcaatcag cttctgaatc cgagaaatta gcgaaagccc aagaatatgt ttctcaacaa
     1741 tctgaaaatg cagagaaaac gatagatatc ctgagacgac agttagaagc tacacagtct
     1801 gagtttggcg ctacatccac agaagcaatg cagatggagg cgaagcttaa tgatgctgaa
     1861 cgtgaatttg aagagttagg acaagctgct aaaaatgtag atacaactaa cttggacgat
     1921 atcggaagca aaatagatat gaataatcta atggaagctt ctgacgtttt aagcgacatt
     1981 ggcgataagc ttacagaatt agggaaacaa gcagtggact ctgctaatag tgtaggtagt
     2041 tcccagagta aaatacaagc taattttggt ttgactaaac aagaggctga agaattaacg
     2101 aatgtagcca gagacattta ttataaaggt tttggagaat cgttagatca gtccacagat
     2161 gcattgattt tggtaaagcg taatttaggc gatttaaata atcaagattt acaaaatatc
     2221 acggaacaag ctatggtcct agaaaacacc atgggcgctg atatggatga aacgttacgt
     2281 ggtgtaaatg gcttaatggt caatttcggc ttgagcgctc aagatgcaat ggatttaatg
     2341 gtttcgggta ctcaaaacgg tttagataaa acgcacgaat taggcgacaa tatggcagaa
     2401 tatagccaat tatggagtca aatgggatat tcagctgatg aaacgttcgg aatgcttcaa
     2461 aatggtttag atgcgggtgc ttataacctt gataaagtca atgacttagt taaggaaatg
     2521 ggaatatcgt taacagatgg tcgatttgag caaaacatgg atatgtttag tgaaagtact
     2581 agaaaagctt ttgaagagtg gaaaaatggc ggaggaacac aaaaagacgt tattaattcc
     2641 atgattcaag attttagcaa tatggatggt caatacgacc aattaaataa agcttctaca
     2701 atttggtctg cacttggcga agataacgcg atgaaagttg tccaatcttt gactgatgtt
     2761 aaccatacat ttgatgatgt tagtggatct gcacaaaaaa tgaatgaaga ttctactact
     2821 ccgttacaag aattaaacgg aaaaatagct gaattaaagg attcattagc tcctataggc
     2881 aacacaatca tagatgcact cgaaccagta attgattttc taggaaagat ggctgatgcg
     2941 tttaataatc ttccacaacc agtacaggat tatgccgtag cgattggcgg attgactgct
     3001 gcatttactt tattaatgcc aataatagtt ggcttcatgg ctctaggtgg tcctactaca
     3061 ttaataatag gagcagttat tactgttatt gctggagtta tagcaattat aaaaaactgg
     3121 ggcgcaatta ctgactggtt taagggaata tggagtaaat tcactgattg gttgggtggt
     3181 acttgggaaa gtataaaaga aggtgcctca tcagtttggg atggagttaa agaaacctgg
     3241 tctggatttg tagattgggt tcaagatatt tggcaaggag tttctgattg gtttggagag
     3301 ctatggagcg gattagttga aggagcttcc aacatctggc aaggagtcca agagacttgg
     3361 caagcattcg ttgattgggt ttcaaatatt tggaacggag tcaaagaagt atggtcgatt
     3421 atttgggcag acattgtagg aattgttcaa ataccatgga ccttaataac gtcattgatt
     3481 caagccggta ttaatattat cgtgggtatt tttgatgtag ctggacagtt attaggcgca
     3541 gcttggcaag ctgtttggac acctatttct gatttcctta aaaacacttg ggatactatg
     3601 acacaatggg taagcatcgc ttggaatgga attgtaacta cattccatac tatatttgat
     3661 ccagtagtgg catggtggaa tggtatatgg acagctatta gtactacggc ttcaaatatt
     3721 tggaattcaa ttagtgcaac agcttctagt atttggaaca gtatcaagaa tacaatcact
     3781 agcttggtac aagcagctgc tacagtaatt caaaatattt ggtcaactgt atctagttgg
     3841 ttaggtggaa tttggaattc aatcagctct acagcatcaa atatctggaa tagcgtgact
     3901 agtagtataa gcaatgctat aaacgcagct aaaagtgcca ttcaaagtgt ttggaatagt
     3961 atatcttcgt ggatcagcgg aatttggaac ggtatcaaaa acactgcttt gaatctttgg
     4021 aatggaatta caagcactat tagctctaaa gtaaacgatg gaaaaaatgc aatttcaagc
     4081 ggttggtcca atctaacagg tattgtttcc gacatattca ataatgttaa aagtacaatt
     4141 gctaacattt gggaaggtat caaaaagact gttagcgctc caattgattg gatcagagat
     4201 aaaatcagta gtatctttga taatttgaat atttctatac cacatattcc gttaccacat
     4261 tttaaattga gcggagaatt caatccattg aaggggaaaa tcccaacgtt gggtgttgat
     4321 tggtatgcga aaggtagtgt gtttaattct ccgaatatta tcggtgtcgg cgaagcagga
     4381 cctgaagcag ttttaccttt gaaaagatct gtgctgcaag aaattggtga tcgtatcttg
     4441 agtagcacat cagtttcatc tagggcacaa acgattcaac ctgtgaataa ctacgaattc
     4501 aatttcacaa ttgatggtaa cgcagatgag gttactatga agcaaacaac tcaacaaatc
     4561 attgatagca ttacaaaagt tcaaaatgat aatgcttcgg catggcgtta aacaggagag
     4621 tatttctcct gtttttttag tattaaaaag gatgtgaaaa aatgactgat tgtatacatt
     4681 ctataatcga tggatttcct gattatttgc ataaattggc tttagctgaa agaccaacca
     4741 taccttctcc aaaaagacag agagttgaaa cttctgtttt aggaaggtta ggtggcttag
     4801 tacaagatta ctcgtttgaa gacatgtcgt ttacattgca ctataactat ttagaggatg
     4861 tggaagacca tcaagcgttc aagcaatcgt tttatatcat gcgtcattgg ttaaattatg
     4921 caaagaaatt agaattctct gatgatccca acgtctatta tgttatccag actatcgata
     4981 ttggggatgc agaaaacgat attgttgaat ggggagagtt cgatgtaaat attactgcga
     5041 aaccattcgc aagagttcaa gaagatgtac ctataaccgt agataaacca cagtcattta
     5101 acttgctgaa taatagttta gaagaaagtt ttccaaagat tatcatcact ccttcagcta
     5161 cttcatgcca gttcatctta aatgattatg tgtttagttt tgaaggctta gtagtaggaa
     5221 ctgacgtagt cattgatagt gatttgatgc tttgctacga agagcaatcg gacggagata
     5281 ttttagatcg gtccaacaaa atgaagacca tgcaatatcc gacattgcaa gtggatatta
     5341 attattttaa ttgtactggt ttgagcaaaa tacaaattta tcgtaatggg ttaaggtagg
     5401 tgaaatagat gatcgataat ttaataacta tttacgataa aaacgacgcg aataatttag
     5461 ctgaacattt atatgatacg caaggtttag gcgctttgtc agactggtta acagctactg
     5521 ttagcaataa attaaacgga gccgagatat ttcagggtac ttatccaata agcggaacta
     5581 atgcagattt gattgtagaa ggacgtatta ttcagtgtta tgtagatgaa aatcgagcaa
     5641 aacagcgtct acggatttat tatgcaaaga cttctgtaat aggcaatacg atagaagtaa
     5701 aagctgaacc tattttcaat gatataagaa aatcggtgtt gaataaatat gacagtggaa
     5761 cagaaaagat cactgctagt caggcatggc aaaacgcaaa aactttagcg aaaccagtta
     5821 ttccttcaca gttttctttc tcgtcattag tagatacgct tgctaatgtg aagatagaaa
     5881 aggcgaattt tttagaattc tttggtggaa aagagggatc tattctagat cgatttcatg
     5941 gggaatttct aaaagataat aacacattac gtcatgaaaa aaggctaggc acggatcata
     6001 aaatcaaagc gatttatact aaaaacttaa ctggtcttga cttagagatt gatgctcaaa
     6061 gtgttttagt tggagtttat ccattcatta gcagctcttc agaaggagaa gacgagatca
     6121 ctctaccaga agaagttatt ttcacggatt acgtggatga ttatcctgct ggatatgttt
     6181 cttttgttga ttttaaagac aaagcgactg atgtagccac attaagggaa gctgctaaag
     6241 actggttgaa aacaaacata gataaacaaa aaccacaagt gagtggttcg attgaattag
     6301 taccattgag gcatcaaaga ggctatgaaa aatttgttga tctagaaaaa gtttcgatgg
     6361 gtgacggagt agatgtgtat catccacagt taaaagtgaa tatgtcagcg agaattgtgg
     6421 aatatacgtt taatgttcta accaattcat acgataaatt agttgtagga aacgtcaaaa
     6481 caaacttctt agaaaacaca gagaataatg tcagcaattt gattaatgat gccattgatc
     6541 aattgaaaaa cggtggcgaa atcagtgatt tactcaatga tattgtagat catcaaactg
     6601 atatgattac tggtcaaaat ggtggttatg ttttattaga tcctaaagaa gcgcctagtc
     6661 gtattttgat tatggacaca ccagataaga ataccgcaag gaatgtttta caaatcaaca
     6721 atgctggtat tggtttttct aaaactggca ttaatggaac atatgaaacg gcatggacgt
     6781 tagatggcgg attcaatgcc tcgtttatta cggctggtga aatagtagga attactatta
     6841 gaggtactac attaattagt gatggtgctg attatagaac aagtattgct aatggcaaaa
     6901 tgacttggta ctcaaaaaaa gttaacaaag atattatgga gctagaagca cgtgattatg
     6961 taagtgctga tgccggtatt gtatcataca ccatgaaaac tggtggtggt ttcatgatta
     7021 gaaatccaca gggtaacttg gtttttagta cgtgggataa tggtaataac agaccgtttc
     7081 tatcttttgg tgcgcccaat ttcaggtata gcaatgctag ttatgtaact tctggcgacg
     7141 gtagttcttt aagcattaat ggtagtgcgg gtaactcatg ggaatttaag gtagctggta
     7201 ggactatgaa atttactagt gatggtatgc taacgttacc aggttgtttt tttggttcat
     7261 gggaagatgg gaaacttgct aggtttgaac aatcaacggt acaagtatat aaagatttta
     7321 ctgttagagg tactaaaaac tcaactgtac caacagaaca ttatggacaa cgactattga
     7381 acgcttatga aactccagaa tattatttcg ctgattatgg ggaagccgtt acaggtgaca
     7441 atggtaaagt tcgtgttgat attgacccca tgtttgctga aacagtaaat ctaagtcggt
     7501 atatgacaca tgtgacacct acagaactag ttttgtgtgc tgttactcat gaagatattg
     7561 accatttcat cattgaaact agtaagccaa acgtattagt tagatggaat ttagtggcac
     7621 accgtctagg gtatgaagat attagattaa aagaggatac agcatatgat agcacagtgc
     7681 ttgaccaaaa acgtttttaa aacgaagaca aggaggtata taaatggcta gcagtttata
     7741 taatttggct ttagatttca gcaaagaatt aaactacacc aaagctatta tggctcgtca
     7801 aggtgataaa gggattacgg tgacggttaa accgtttcta aatggcttgc agatggatac
     7861 gagtggcgga acatttactt taaaaggaac aacaccatct aaccgttacg tagataatgt
     7921 tgcaactagt gtaactagtg aagaagtcac gttttctctt gatggcacat ttatgagtga
     7981 agcaggatat tataaacact gctacgtaga atatagaaaa gacaatcaaa ttttaacaac
     8041 gcaagatatc atttttttct cactaggagt gtctgacatt tcgcaaggcc aagccgatga
     8101 atatgtttcg caattagaag agttgattcg aaagtacaac gaaacttttg atgcttttat
     8161 ggctgaaatc aaaggtagag tggatagctt aaatcaacag attactgatt taactggtca
     8221 agctaaaacg ctacaagaca agttagatgc tctgaaagaa gaaatttcta agttaggtaa
     8281 cttacaagtg atgtacagta acagcatcga cttcgggggc tacgattata gcgggaatcc
     8341 gaatttaatg gctaatataa acgctgacag cttctcgcaa ggtagtggtg ccttatctgt
     8401 tgtagatgat ggtgacgagg tagtagtcac gcttgaccca aacaataaat tagagatatt
     8461 caaagcgaaa agtcaaccag ctctggtagt aggaaaaaca tataccatga gcgtagaaat
     8521 tatgctagaa ggcgatttca ctggagaccc tagcaagata ggcctaagat atattaaaat
     8581 gcctaactgg gtatcagggc tatatacgcg taatacatta actgctacta agggtgtatg
     8641 gcaaaaacta actggcaccg ttaaaattac tgctgcgagt gataacgctg aaagctggtt
     8701 tataatgcta caaaataaag acgctaataa cagcctatcc ggcaaactac gtttgagaca
     8761 cgctaaactc gaagaaggct caacagccac accatatcaa ccaaatttac tcgatgcgcc
     8821 gtattatttg agtaaggtgg ctttgggtga gaatattgct gatcctgcag ttagcttccc
     8881 aattaaaacg agcgcatacc gtttatacgg tgtgaacatg ctagaagagt ttaaagttgg
     8941 tcaaagatat acaattacaa taaaaggaac taaaccagca actcaagatt tttgggctta
     9001 caacggtggg aatatttcat tagagagaat gactccagta gaagggttag ttgacgtgtg
     9061 gacttgttca tttactatat taaaactaga tagtagttca ccaagtctat tgagtatata
     9121 tcaaacacca caatcaacag taggctcatg tcaaattgac tggctcaaga tcgaaaaagg
     9181 cgacacacga accccgaata ttgagcaata taaataccga ggaatcggca tgcgagactc
     9241 aaacaatcca aaagattatg tttgggatct agcaccagaa tatgtcgaag ataatttggc
     9301 cacagatatt aaaatttctg aaattactgg taaagcaaac aattataccg atgggaaagt
     9361 atcggagatt aattcgcagt tgactgcttc aattaatgaa gtagacacca cagctaagga
     9421 tgctcaaaca aaagcgaatg ctaatgcgac tgctatagat gaattagaca ataagatcga
     9481 tgaacgcatt aatgatacag ctactaccac attaacagtt acaaacggga ataccgga
//
LOCUS       DAHHZJ010000114         9501 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.115, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000114 DAHHZJ010000000
VERSION     DAHHZJ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.115"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            207..2678
                     /locus_tag="NBL02_002085"
     CDS             207..2678
                     /locus_tag="NBL02_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCD2572762.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HANQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2781..4517)
                     /locus_tag="NBL02_002086"
     CDS             complement(2781..4517)
                     /locus_tag="NBL02_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HCD2572763.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4514..5218)
                     /locus_tag="NBL02_002087"
     CDS             complement(4514..5218)
                     /locus_tag="NBL02_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD2572764.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5349..5852
                     /locus_tag="NBL02_002088"
     CDS             5349..5852
                     /locus_tag="NBL02_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HCD2572765.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5812..6150
                     /locus_tag="NBL02_002089"
     CDS             5812..6150
                     /locus_tag="NBL02_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HCD2572766.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6171..7589
                     /gene="ffh"
                     /locus_tag="NBL02_002090"
     CDS             6171..7589
                     /gene="ffh"
                     /locus_tag="NBL02_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCD2572767.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7701..8279
                     /locus_tag="NBL02_002091"
     CDS             7701..8279
                     /locus_tag="NBL02_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCD2572768.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8283..8804
                     /locus_tag="NBL02_002092"
     CDS             8283..8804
                     /locus_tag="NBL02_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCD2572769.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8883..9437)
                     /locus_tag="NBL02_002093"
     CDS             complement(8883..9437)
                     /locus_tag="NBL02_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCD2572770.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
BASE COUNT         3087 a         1753 c         1974 g         2687 t
ORIGIN      
        1 gagttgaaac gaagaaaaag tgttagtaga aaatcgcctt cgattttcca ccaacacaaa
       61 atattataga accctttttt tttgctatat gacaggaatg aaaggtgttt gcaagcttaa
      121 tgtgaggaac actttccata tctgttctat actggatagg agaagtttgg aaatatccag
      181 aaaacgaaat caatcgaggt agccacatga agaataaatg gataaatcga agtaaaaaaa
      241 agaaagtaac acaaagatca tcagataaag tcatccgtct ttttccatcg tataaagaag
      301 gattaacggc agatcaagtc aaggaacgaa tagaaaaagg tgcagcaaat aattcagtcg
      361 atcccacatt caaaacaaat cagcaaatcg tattggaaaa catatttacg tatttcaatt
      421 tgatctttct tatcttagcc attttgcttt gtttagtaga atcctataag aacttgactt
      481 ttttaccagt tatcatagcc aatacaggaa tcgccatttt tcaggaaatt cgatccaaaa
      541 agatcttgga cgaactaaat gttttaaata cagcaaaagt caatgtcata cgagacggga
      601 tagaacaaac aatagatata gaagaccttg taatagatga cattgttatt ttagagactg
      661 gatatcaaat tccagcagat gcagaggtcg tggaagggaa actgcaagtt aatgaggctt
      721 tattgacagg cgaagcagat gaaataacca aagaaatagg tgatccatta atgtccggca
      781 gtttcattgt ttccggaaaa gcttacgccc gattagacaa agttggagca gattcgtata
      841 tttcccagct gacagtcaaa gccaagacaa tgggagaagg agaacagtcg gagatgattg
      901 cttctcttaa tcaattgatc aaatggattg ggatcatcat tattcctatc ggtatcctgc
      961 ttttttctca aagttacttt tttaatggaa acacgatcaa agaaagtgtc gttgcaatgg
     1021 aagcagcatt gatcggtatg atacctgaag gactgtactt gctgacgacg attgcattag
     1081 cattaagtgc gactaaacta gcaaagcaaa gagttctttt acacaatatg aaaagcattg
     1141 agacattggc aagggtaaat gtcctttgtg tagataaaac aggaacgatc acagaaaata
     1201 aaatgtctgt tcaaaaaatg atcgtctcta aaaaacaaga aataatcagt cagtcagatc
     1261 aattggaagc atgcattgct gactatgcaa aagcgatggc aggagacaat gcaacaatgg
     1321 aagctgttaa agcatatttt acaaaaacca ctgatcaatc ctatcaccat gttattcctt
     1381 tttcttctgt ccaaaaattc agcagtgtag cgttggcaga caaggtgtat gttttaggag
     1441 cgcccgaaat ggttttgcgc gatcaattgc cggaatatgc ggaagagttt gtttctttcg
     1501 ctgaaaaagg ctatcgcgtg ttggtctttg gtgtgtacga tgggattctt gaagaaccag
     1561 tattgacaga agcagtcaca ccattaggct atattctgat tgctaatcct atcagaaaag
     1621 aagcaagagc cacatttgat tacttcaaaa aacagcaagt agccatcaaa gtgatttctg
     1681 gagataatcc gctcaccgta tccaatgtag ctgtccaagc agggatttca catgctaatc
     1741 agtacgttga tgcatctaaa ttatcagaag aagaatatga agcagctgtc gaaaaatata
     1801 cagtttttgg acgcgtcaag ccagaacaaa agaaaatctt cgttcaattg ataaagcgta
     1861 acaatacagt agctatgaca ggtgatggcg tgaatgatat tctagccatg aaagaagcag
     1921 actgcagtat agcgatggct tccggaaatg aagccgccat gcaggcctca caagtggtct
     1981 tacttgattc tgacttttca cgcatgccag aagttgttgc agaaggacgg cgcgttgtga
     2041 acaatattga gcgatcagcc agcttgtttt tagtgaagaa tattttctcc tttttgctct
     2101 cactgttttc agtgatcttt gctctgacct atcctttaga accatcacaa atcacattga
     2161 ttagtttgtt tactatcgga cttccatctt tcttgcttgc tctagaagaa aacaaaaagc
     2221 gaatcagagg gaaattcatc atgaatgtga tggaaaaagc tgtgccaggt ggactaacag
     2281 atatgatcgt agtcggtgcg ttggtgatct gcggggtgac tttgaatcta aataaaacag
     2341 atgtttctac agcatctacc atgcttttga ttgcagtagg gttcctcgtt ctctataaaa
     2401 tctgttcacc gctgaataaa ttcagaagtc agatcattct tttctgtgct agcggtaact
     2461 ttttttcggt catctttttg cataagctgt tttcaatcac cggtatctca gctgtatcac
     2521 ttttgctgtt gagcattcta tttttctcgg cagattcgat ttttaggcat ttgaccacat
     2581 tggtagaata tttgtttgaa cgtaaaaatg aaaaagagca aaagccgttt agtcgcgtag
     2641 ctaaaaaatt tctatcgata tttaaacgag aaaattgacg aaaagtatct caaaaaagag
     2701 taaaaaagaa caaccactcg tgtatctttc gaagcaggat gagtcatcca actttaaaac
     2761 gcacgagcag ttgctctttt ttacattttc ggaatggtaa cggtgaaagt cgaacctgtt
     2821 ccaagatgac tgtctacagt aatcgtacca ttaaggagtt gcacgtagtc ttttacaatc
     2881 gctaacccta atccggtccc gcctgaatgt cggcttcgcg ccttgtctac gcggtaaaaa
     2941 cgctcaaaga tccgttcctg atcctcaatg tcgataccta taccaaagtc ttggacagaa
     3001 aacgatagat catctgttgc ctgatagcgg ataataatct cgctgtcgct ttttgaatat
     3061 tggacagcat tttcaatcag atttttgata attggataaa ggatatctgt tttagtagta
     3121 aatggttcgt tctttccaat caatcgaatc gtcaaccgtt tcttttcgat gattggctga
     3181 taattttgaa cgatttgctg gaaataaggt tctgtataaa ttgtctggat ctcgtaagaa
     3241 ttctcgccat cttttgacag ctggatgatt tcccgaatca gtttatctaa ccggatcgca
     3301 tctttttgca tgatctcaag aaaagaagtc agagtttgcg ggtcatcttt tgcaccatcc
     3361 agtaaagtct cggtaaaacc aatcagtgaa gtcacaggag tttttaattc gtgagagacg
     3421 ttgccaacga aatccttttg tagcttttct aaacgtcgca ctttcgttaa gtcatacgcc
     3481 acccctagaa tctgtccggt accttctgaa ttagtaaagt agcgcaaact caagtctaat
     3541 gtctgccgac cttctgtaag ggtgatctcc tgatggatca gcggatgctc aggagtcact
     3601 tgatgaatca gctgaatgag ttttggctct tgaatgactt ctgtgtaccg ctgttcaggt
     3661 tgatagctta ccacgtttaa atgtagctgc atctttggat taagcagtag taaacgagaa
     3721 tcagcatcat caatcaggaa tacgccaatc atcagttcgt tcagtaacgt atataattgc
     3781 tcttctgtgg tagtacacgc ccgatacatc ttactcattt gttcgctcaa cgcattaatc
     3841 gtctgataca attcttccca ttgatcgggt gtttgcatga ttacttccgc ctgattagga
     3901 ttttgcacca ttttttttag tacgggcagc acggttttca atggctggtt tttttgatag
     3961 atcagataat agatcattcc tgcaagaaca aggaagaaag acagaaagaa gaaaaagacc
     4021 caacggcgaa aactctcagt tctcggtaaa aaaacgcttg tcggttctgc gatacgtaaa
     4081 atggcttcta actggccact ttttttgact ggtaatgcga catataaaag atcctcattc
     4141 aaagtagtac ttttgcgcaa agcgctacct aggttcccac cattcaatac ggctttgatt
     4201 tcgggacggg agtttcgctg ttcattcaat gtttcattgt ttgtgtcaaa taagatcttt
     4261 ccagtagcat ccattaaagt aatccgttcg ttcgactgat ggacgaattc ttccaaagca
     4321 gttttgtttt gcgctgcttg gaggttttcg acatctaatt gccggatcag cagttgtgct
     4381 ttttttgtca ggtagctttc ttgctgttcc acgatctgct gttgaaaata atgggaaatc
     4441 aattgccagc caccaaaaaa caaactaact aaaagaataa gaaagaagag acgttcataa
     4501 atttttttcc atttcatcgt ttgggttcct gaaacttata gccaaatcct ctaatagtca
     4561 ccaagtactt tgggtgtttt ggatctcgct cgattttttc tcgcaaatga ctaacgtgta
     4621 catctacgat tcggctttgt cctgcgaagt caaaattcca aatgcggtct agcaatgtgt
     4681 ctcggtcgat cacacggtct tttcgtttca tgaaataaac aagtaattca aattctttcg
     4741 gtgttagttc aatcagatgg tcgtctacgg tcacttgata attggtcaaa tcagcagtga
     4801 tttgtccaat cgatagatat tcaggctcag cttcatcttt tgattgctta cgaggttcaa
     4861 ttcgtctgaa tattgctttc atacgggcta atacttcgcg aggactaaat ggtttggtca
     4921 aatagtcgtc agcacctatt tctaggccaa tgatccgatc cacttggtca tctttagccg
     4981 tcagcatcaa aatcggtgtt tcattttttt cttgacgcag ccgctgagtg atctccatgc
     5041 catccatcgt tggaagcatc acatctaaaa tgatgaaatc aaatgattgt tctaatgcta
     5101 attctaatcc ttcgcctcca tctgtggcac tggttacctt gtagccttct ttttccaaat
     5161 tgaacgtcaa gagcgttaca atagaaggtt catcatcaac aactagtatt tttttcattc
     5221 aaatctcctt taaataggtt tcattctgtt tatattttag catagaatca gtacaaaggg
     5281 ctattttaca aaagaaataa atggtataat aggaaggcac tttgctgaat agaaaggaag
     5341 agcagtcaat gattggaatc agtgcatgtt taggcggtct ggcctgtcgc tatgatggac
     5401 agaaaaaaga agtaccagaa ttgatggaac tagttgaaac tggagaagct gtcatggtct
     5461 gtccagaagt gatcggcgga ttaccaattc ctagagatcc cgctgaaatc attggtggaa
     5521 atggattcga tgtgtggaaa gatcaagcaa aagtatggac gacaagcggc gaagatgtaa
     5581 cggaagcgta caaaaatggc gcgatcaaag cctatcaaaa attgcaggaa aaacacattg
     5641 atctagtcat catgaaggaa aaaagccctt catgcggaac ccattcgatt tatgacggaa
     5701 gtttttccgg aataaaaaaa aatggtgccg gagtggctac tgcttatttt attcagcaaa
     5761 aaatgactgt gctctctgat gaagaatggc tgaaaactag aggtgagcag aatggaaatc
     5821 gaaaagacca atcgaatgaa cgcgctcttt gaattttatt cgaccttatt gacagaaaaa
     5881 caaatgaact acatggaatt atattatgca gatgattttt cattaggtga gatcgcggaa
     5941 gaatacgaag taagccgaca agcagtatat gacaatatca agcggacaag caagatcttg
     6001 gaggattatg aaaaaaaact ccatcttttt tctaattata ttgttcgaga acaggtattg
     6061 gaaaatatga ctgcatatat tacagataag tacccggaag ataaaaaact acttgaatat
     6121 gtacagcaaa ttcaagcaat cgaagaataa ataaaaggaa tgaagaaaat atggcatttg
     6181 aaagcttaac tgaacgtctc cagcaggcga tgggaaaaat aagaaaaaaa ggaaaagtat
     6241 cagaagctga cgtaaaagaa atgatgcggg aaatccgcct tgccttacta gaagccgatg
     6301 ttaacttgca agtagtcaaa gattttacta agcgcgtacg cgaacgtgcg gtaggggcag
     6361 aagtgctgga aagtttatca cctgcacaac aaatcgtaaa aatcgttgat gaagaattga
     6421 cgatcacact tggttctgaa acagcagagt taaataaatc accaaaaata ccaactgtta
     6481 tcatgatggc tggtcttcaa ggggcaggta aaacgacctt tactggtaaa ttagccaatt
     6541 atttaaagaa aaatgaaaat gctcgtccgc tcctaatcgc aggggacgta tatcgaccag
     6601 ctgcgatcga ccaattgaaa gtattaggtc aacagctaga cgttcctgta tttgatatgg
     6661 gaactgaagt cagcccggta gagatcgtcc gtcaagggat ggagttagca aaagaaaaga
     6721 aaaacgacta tgtattgatc gatacggcag gacgtctgca tatcgatgaa acattgatgg
     6781 atgagttgaa acaaatcaaa gaattgacgc aaccaaatga gattttgctt gttgtcgatg
     6841 ccatgactgg tcaagatgcc gtcaacgtag ctgatagctt caaccagcaa ttaggtatta
     6901 caggggtcgt catcactaaa cttgatgggg atactcgagg cggggcagca ctatcgatcc
     6961 gctcagtgac aggcgcacca atcaaattca tcggttctgg tgaaaaattg actgacttag
     7021 aagtattcca cccagatcgt atggctagcc gtatccttgg tatgggggat atgctgacat
     7081 tgattgaaaa agcgcagcaa gactacgacg agaaaaaagc ggaagaactt gcacagaaaa
     7141 tgcgggaaaa ttcattcgat ttcaatgact ttattgaaca gcttgatcaa gtgatgggta
     7201 tgggcccact ggaagatcta atcaaaatga tcccaggaat gaatcaagtc cctggaatcg
     7261 aaaacgtcaa ggtagatcca aaagatgttg aacgcaaaaa agcgatggtt tattctatga
     7321 cgccagcaga aagagcaaat cctgatctgt tgaacccaag ccgtcgtcgc cgaattgctg
     7381 ccggttctgg aaacagtgtt gttgaagtga atcgaatgat caaacaattc aaggaatcaa
     7441 gaaaaatgat gcagcaaatg tccaaagggg atatgaacat cccaggtatg gaccaaatgt
     7501 ttggttcagg cgtcaaagga aaactaggca aaatggcgat gaatcgaatg atcaagaaaa
     7561 ataagaagaa aaagaaaaaa cggaaataag atgaaaaaaa cagcttcggt acagactgaa
     7621 aatgtctact gccggagctt ttctatcagc aatttttatt ctttactaat acaattactc
     7681 taaacgaaga ggtggcacag atgagatgtc cgtggagtga acaaacagaa agtatgcggc
     7741 aatatcatga tactgtctgg ggtgttcctg aatatgatga ccaaaagctg ttccgaaaat
     7801 tgatgcttga tattaatcaa gccggattga gctggcagac gattttaaat aaaagtgaag
     7861 cattcgatga agcatatgat cgattcgata tcaaaaaagt cgcaaattat ggagaaacaa
     7921 aaatcagtga actaatggaa aacaaaggaa ttattcgtaa ccgaagaaaa atcgaagcag
     7981 ccgtccaaaa cgccaaagca gtcttgaaaa ttcaagaaga aatcggcagt ttttcagagt
     8041 atctttggtc gttttctaat ggaaaaatcg ttgattcttc ttatcattca caagaagaag
     8101 tcccaacaac aagtcctctt tcagatgaat tagcaaaaga tctaaagaaa agaggattta
     8161 aatttatcgg aagcacaaca atctatgctt ttttagaagc tgtcgggatc atcaatgatc
     8221 accttgacag ttgtttccgc aaagaagagg tcgtacgtat cggcagaaag ggcgaataaa
     8281 gcatgcgaaa aatcatattt tatggtgcta taagtttaga tggctatttg gcaacgaaaa
     8341 cggatgatct tcaatggctt tttgacactc caacaggtaa gcaaactact tatgaagctt
     8401 tttatgaaac aatcgatacg accatcatgg gcagaaaaac ttatcaggaa gcaaaaaaat
     8461 atttggagac cgaagcaatt tatccagaga aaaagaattt cgttttttcc acccacgcac
     8521 aattacaatt gccagatgct gaggtcgtaa acgaagatcc tgttgctttt ctaaaaaatc
     8581 tacaaatgac agaaggaaaa gatatctggg ttgtgggtgg cggtcgattg atcaaaccgc
     8641 tattggaaca tcaaatgatt gatgaatggt atatccagat tacccctgta ttattagggg
     8701 acgggattcc tttattccaa aaaggaacat atgaagcgcg ttttgaaatg ctagacacga
     8761 cacgattcgg tgaatttatc gaattgcatt atgtacgaaa gtagaagtaa ataaaataaa
     8821 aaaggcaact atatgcagaa atgtcctgta catagttgcc ttttttatta ccgttatgct
     8881 ttttactctg ttaatttgtt agaaaaatct agatacgcat ctaaaatcgt ttggaagaaa
     8941 cttaatgttc cttcaacgat attaccattt tcatcaatta gattcgcaac gtttccgata
     9001 tatgcttccg gttgctgcaa ggttggcaca ttcaaaaaga caagtgattg gcgtagatgg
     9061 tggtttgcgc caaagccgcc gatcccacct ggtgatgcag taacgactaa acctggttta
     9121 tgatcccaaa cactgtgtcc ataaggtctt gagcctacat ctaaagcatt tttcaatact
     9181 gctggcacag aacggttgta ttcaggagta acgaaaacga caccgtctag tcccctcact
     9241 tcctcacgaa aacgagtcca ttcagcagga gcctgttctg gagtttcgag atcctcattg
     9301 taaaatggca gatcatcgat ttttacaaat acactttcgt acccttcagg aagcaatttc
     9361 gcaaaagctt ctgctacttt tttgttgtaa gaatcttttc ttaaactacc aataaaaaat
     9421 ccaatttttt ttgtcatacg atttttcctc cttcaaacta tctataataa tcttacaaaa
     9481 gataagtaac tggagcaaat a
//
LOCUS       DAHHZJ010000115         9432 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.116, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000115 DAHHZJ010000000
VERSION     DAHHZJ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.116"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            364..2199
                     /gene="tdc"
                     /locus_tag="NBL02_002094"
     CDS             364..2199
                     /gene="tdc"
                     /locus_tag="NBL02_002094"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HCD2572771.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2504..3982
                     /locus_tag="NBL02_002095"
     CDS             2504..3982
                     /locus_tag="NBL02_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD2572772.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3948..5279
                     /locus_tag="NBL02_002096"
     CDS             3948..5279
                     /locus_tag="NBL02_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD2572773.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5393..7945
                     /locus_tag="NBL02_002097"
     CDS             5393..7945
                     /locus_tag="NBL02_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HCD2572774.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7952..9391)
                     /locus_tag="NBL02_002098"
     CDS             complement(7952..9391)
                     /locus_tag="NBL02_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD2572775.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
BASE COUNT         2971 a         1559 c         2008 g         2894 t
ORIGIN      
        1 aaaaaaggtt ttttatgtta taaaatttgg aaaacagata attctattaa aaaataaatt
       61 gcgaattttt aacaatataa aaaaataaaa atatgtttag tgaatgaagg acagtcattt
      121 tatttgtttg ttcgcttttt ttaggttatc tattttagaa gactgattta ttgaaaaatt
      181 gttcgctttt ttgcgttgaa ttatcagaaa aatccgactt tatttatttt tgtttgtaaa
      241 aatctacgtg gatttgatgt tataaaaaaa aaattctcat ttttggcgct atagtgaaga
      301 ggtgttacag ataaacacat gtatctgcaa gacattgacc aacaaaatat ggagggaaga
      361 aaaatgaaag atatggatat caaggccgtc tttataggcg acaaagctga aaatggacca
      421 gtttataaaa tgttgttgaa caagatggta gatgaacatc ttggatggag agaaaattat
      481 attccttcag atatgcctgc aattagcgaa ggagataagc tcactccaga ttatctagcc
      541 acccgcgacc atatgataga ggtcttggat gaagtcagtc agcgattacg cgcaggatcg
      601 atcccatggc attcagcagg ccgttactgg ggacaaatga atgctgaaac attgatgcct
      661 gctttattag cttacaacta tgcgatgctg tggaacccaa ataatgttgc attggaatct
      721 tctatggcaa cttcgcaaat ggaagcagaa gtaggacagg attttgcttc attattcaat
      781 atgacagacg gatggggaca cattgcagca gatggttcta ttgctaatct tgaaggatta
      841 tggtacgccc gttgcatcaa atcgattcct ttagctgtca aggaagtgct gcctgaaaaa
      901 gtgaaaaaaa tgtctgagtg ggaacttttg aatttatctg ttgaagaaat tttggaaatg
      961 accgaaagct ttacggacga agaaatggat gaggtcaaag cagcttcttc ccgtagcgga
     1021 aaaaatatcc aaagattagg gaaatggctg gtccctcaaa caaaacacta ttcttggatg
     1081 aaggcgttag atatttgcgg agtcggctta gatcagatgg ttgctatccc agttcaagag
     1141 gattatcgaa tggatattaa tgctttggaa aaaacaattc gtgaattagc tggacaaaaa
     1201 atcccaattc taggtgtggt tgctgtcgta ggaacgacag aagaaggtca agtagacagt
     1261 gtagataaaa tcgtccaatt aagagaaagg ttaaaagatg aagggatcta tttctattta
     1321 catgttgatg ccgcatatgg cggctatgcg cgttcactgt ttctaaacga agcaggagaa
     1381 tttgtgccgt atgcttcctt agctgaattc tttgaagaac atcatgtttt ccaccactgc
     1441 gtaacgattg acaaagaggt atatgaaggg ttcagagcta tttcagaagc ggattctgtc
     1501 acgatagatc ctcataagat gggctatgtc ccttatgctg ctggaggaat cgtaatcaaa
     1561 cataaaaata tgagaaatat catttcttat ttcgcgccat atgtgtttga aaaatcagta
     1621 aaggctccag atatgttagg agcttatatt ttggaaggat caaaagctgg agcaaccgcc
     1681 gccgcagtct ggacagcaca tcgtgtattg ccattgaatg tgaccggata tggtcaattg
     1741 attggggctt caatcgaggc agctcaaaga ttcagagaat ttctcgatca tctgactttt
     1801 actgttaaag gaaaaacaat cgaagtctat ccattgaatc atcctgattt taatatggtc
     1861 aattgggtat tcaaagaaca aggctgtaca gatttgaacg ctatcaatga attaaatgaa
     1921 aaaatgttcg atcggtcttc ttatatggat ggcgatgttt atggtgaacg gtttattact
     1981 tctcatacta catttacaca agaagattac ggtgattctc cgattcgttt tgttgaaaga
     2041 atgggtctaa cgaaagaaga atggaaaaaa gaacagaaaa tcactctgct acgtgcggct
     2101 atcatgactc cttatttgaa tgatgatcga attttcaatt tttatacaaa aaaaatcgcc
     2161 aaagcaatgg aaaagaaact aaacgaaatc atccaataga actgattttt gtgtgaaaga
     2221 ataacaagag acatatggag aacagacaat tatttgcttc atcaaaacaa ttgtatttcc
     2281 ccctgttttt catatattcc tcgccaagat attctttttt ataaaacgtt ctataagttg
     2341 gatcgttcgt ttcatgtatc aaacccagtt cactaagatc gttcaatgga tgtttcggtg
     2401 atactgggtt ttgtactgaa tcgatgaaac cttgtcattt ctgttcttga acaaaaataa
     2461 aagaaaacaa aaacatatgt cgctattgaa agaaggaata aagatgaaag atactggttc
     2521 atcaaagcaa atcacgacca tgacctttat cggaatgacc tgcgcccttg tagcaagtgt
     2581 acgaaatatt ccagatgtag cagcaacgag ctggacgatg attttttaca tgctggttgc
     2641 agtattattg tacgcttttc caatctcatt gatctctggt gaatttgctg gaatgtttcc
     2701 ccaaaaaggt ggtccagaac tttgggtatc gaatgctcta ggaaaaaaat gggggttcgt
     2761 tgtctcatgg ctgttatggg tccaaatgtt tcctgggatg gtgatggtcg cttcagcact
     2821 cgctccgttg tttggtaata ttatcgataa tgtgccgctt ggtttgaaca gcaaatttac
     2881 attagttgtc attttagtcg tttattggat catcactttt ttgaatttga aatttgatat
     2941 ggcaaaaatc ggcggaaaag tcggtgtttg gttaggattg tacgtgcctt tgacgatgat
     3001 gctcttatta gggtttgctg cgtggatcaa agttgggatc gtaccaaccg gaacattagg
     3061 cagtttttca tgggacaaat taataccaga tactacgact gcatcctcat ttgtttattt
     3121 tgcgccgatc atgtttattt ttactggtat cgaaatgtcc tctgtgtata tcactcgatt
     3181 ggaaaatcca gttaaaacgt atattcgcgg agtatttgcc gcgttaattt ttatattttt
     3241 cgtcaatatt ttgaatgctt tagttgtagc aaatgtcgta ccaaaaggac aaatggaatt
     3301 gaataatatc gcgcaatcta tttcgattta ttgccaaatt cttggtttgc cacatcttat
     3361 tgtgaatctg tttagtttac ttgtcttcat tggtgtagca gtccaattat ctgcttgggc
     3421 ctcaggacca gcaaagacag tgacagaaag tgctagaaaa ggtgcgtatc caccaaaatt
     3481 caatttttgg aagacgaatc aatttgacgt atccaaatcc gttattttga ctcagtctgt
     3541 cattatctca atctttgcac ttttttattt gctgattcct ggcgtcaatc aagcattttt
     3601 gatgctcgta aattcaacta ctgttatcta ctgtatcgtc tatgtcatca tggggatcgc
     3661 agttttgaga ttgcgttata cacatgcaaa gttgaatcga ccatttagaa ttggaaaaaa
     3721 agagaaaaag agcaatcttg gagtttggac tgtcgttatt gttttatttg cggcaatcgg
     3781 attttctgtc ggactgacga tgaaagctgg aacatggatc aatctaattg cagtcacagc
     3841 catcagtgtc attttgtttg tagtaccact ctgtattgaa aaaataaaga aaccttcttg
     3901 ggaacaggaa gtcgccatgc tgcttgaaaa agaaaaaggt gaacttgatg gaaaaaccgg
     3961 caatgaaacg gactctaact aatcgaaata tccagttaat cgcattaggt ggcgcaattg
     4021 gaacaggatt atttttaggt tcagcaaagg caattgcatc agcagggcca gctgttattc
     4081 tcgtttattt gatatgcggt ttggctattt atggtttgat gcgggccatg ggtgatttat
     4141 ttttagcaaa tagtacattt aactctatca gtgatttcgt tgactattat ttaggcaaaa
     4201 agtgggcatt ttttgtcaat tggacttatt ggctttgctg gcttggagct ggacttgcgg
     4261 aattaacagc aataggattg tatatccatt tctggtttgt acatgtacca gctttgataa
     4321 gcggaggtag cgcactaatg atcttaatgg cagtcaacct tgttgcagtc aagtggtttg
     4381 gtgaattgga atcttttttc tcaatcatca agatcttggg gattttcctt tttatcttat
     4441 tgggtatagg attgagtgtc tcagattttc atacccagtc tatcggaatc aaagaaaatt
     4501 ttggacagat cgatgaatta ctaccgtttg gctttgctgg aatggtagtc tcatttccta
     4561 tggttttatt cagttttgct ggaattgaaa tgatcggctt aaccgttggc gagacaagtg
     4621 atccgcaaaa gaatttgaaa aaagcaatca actgtctgcc ttggcgtatt ctctttttct
     4681 atataggaac gattttgtcg attttatttg ttgttccatg gcattgttta gatttaaaag
     4741 aaagtccttt agtgactatg ctgcaagtga tccattgcca aggcggagca gcaattgtca
     4801 atctaatgat tttggttgct tctttgtcct cagcaaatag tgcagtctac agtactagca
     4861 ggattttata tcaaacagca aaagaaaaaa gtttgcccca ttggttttgt gcgctttcta
     4921 agaaaggcgt acctttacac ggtattttaa ttacaggaat gttatttttt gccggtcttt
     4981 gtctacattt ttttgtagct gatagtcgat tgttttttca gcttttggca ggcatgacaa
     5041 cgagttgttt tctatttgtg tggtcttcga tcttgtgtgc acatgttcaa tttattaaag
     5101 aaaagaaacg gaacactaca caaagatata aagatcttgc tttgttggtg ttttttactt
     5161 caattgcttt cagcttgtta ttcgaaagca cgacacgctt tattcctttt ttattggtta
     5221 tctggtttgt attcttaaat tttgtatata aaagaataag caatagaaat atcagttagg
     5281 cccagataca ttttgcaaaa cgaattattt cagacccccc tgaaataatc gatacgcctg
     5341 aaaaaacttc cctgcgtata ataacagtat caattaaaca ggaggggata ggatgaactg
     5401 gtacagattg acggtagaag atgtattgaa aaactatcaa acatcaacag aaggcttgtc
     5461 gcaagaagaa cgacagaaac gcctgactga tcaaggatat aatgaaataa aagcaaggca
     5521 gcagacaaaa cgatggaaaa aaatagcaaa gcattttact gatctgctga tgatcgtgtt
     5581 gatcattgcc tcttttttga aatttgcttc tagtgaatat attgaaggaa gtattatttt
     5641 attcgtagtg atcgtcaatg gactcgttag ttactggcaa gaaagaaaag cggaagaatc
     5701 attgaatggt ttgaagcagc tgatgggaca agaagcgata gtactatcaa acgggatgca
     5761 agaaaaaatc cctgcgaaag aactggtaaa aggagatgtc attgtcttac gtgccggaga
     5821 tgtcgttcca gcagatatcc gattaatcga agcacatgat ttattagtgg aagaatcgat
     5881 tttgacggga gaatcagaag catctgaaaa aagtcatgcc gttttgacag aagaagaatc
     5941 gatcggcgac cagaaaaata tgggtttttc aggaacgctt gtacaatctg gttctgcagt
     6001 cggtgtcgtt gtagaaacag gaaacacgac agaaatcggt aaaatcaatc aagcattgca
     6061 atcggtcgaa caacaaacga cgcctctgat taaaaaaatc aaccgattaa acaaacaaat
     6121 ttttcaaggc attctttgtt tgagcctatt cttagttatt tttacgacat ttcgatatgg
     6181 tatggattgg catatccttc tttcggctac gattgctttg gtggtgtcaa tggtacccga
     6241 aggattgcca gctgttttga ccatgattct atccgttgga gtacatgaaa tggcaaaaga
     6301 aaaggcgatc atcaaaggac ttccttcggt agaaacatta ggttctatga cagtgatctg
     6361 ttcagataag acaggaacat tgacaaaaaa tgaaatgaca gttgtggatg tagctgcaaa
     6421 agaagaagcc tgcgtgttgt ccattatgaa aaactgtcag gaattaaaaa caaaagaaga
     6481 acagaaaaca gaaaatttaa gtggcaatcc gacagaagtt gcgctgattc gacacacaga
     6541 aacagccaat ctacctttga aaaaaataga agcaaagatt ccgttcagtt ctgaatacaa
     6601 atatatggca acgatgcatg cggaggaaaa aggagcggtg atttacgtca aaggtgcacc
     6661 agaagtatta tttgctaaat caactctttc ttctgcagaa caagaagcat ggagtcagac
     6721 agctgcagaa tttgcgagaa aaggtcagcg tgttttagga tttgcttata aaaaagtaga
     6781 ttcgaaacag gaactcacgc atgagacttt aacccaatta acttttgcag ggatcgccgg
     6841 attgatcgat ccgccaaaag aaagtgcagt caaagctgta aaggaatgcc aacaagcagg
     6901 gatctcagtg aaaatgatta caggggatca caaagacaca gcaaaagcga ttgcggaaca
     6961 aattggctta aaacatactg ccaaagtttt agaagggatc gatttggatc ttatgtctga
     7021 tgaagagttg attcagcaag tgccaatagt cgatgtcttt gctcgaacga caccagaaca
     7081 taaactgcga attgttaaag ctttgcagaa aaatggagaa atcgtcggaa tgaccggaga
     7141 cggcgtaaat gatgcacctg ctttgaaaag atccgatgta ggaattgcta tgggaatcaa
     7201 aggtagtgaa gtaagcaaac aggcagctga tatggtttta ggagatgata atttccatac
     7261 gattgcaaaa gccgtaaaag agggccggcg gattttggat aatttgcaaa aaaccatcaa
     7321 tttcttcttg ccaactgcat tagctcaagg attgatcttg atttgggcac ttatgctgaa
     7381 tcgcccgcta cctttgtccc cagtacagat tttatgggtc aatatggtaa cgacaatcac
     7441 tttatcttat gcactagggt tcgagtcgcc agatccagaa atcatgaagc gcccaccaag
     7501 agatccaaag caaggaatcc tatctggtta tcatttgttt cggatcatct atgtctccct
     7561 actgatcatg gttcctgcct atttattggc tatgcagttt gaagggcaaa gcttacagca
     7621 aaccattctt ctgcaaaata tcgtcttagc tcaagctgtt tacatgatca attgcagaga
     7681 gttgtccaag ccgtcattga accaaggtct tttgcaaaac aaagcgcttt ttctttcatt
     7741 aggtatcctt gctttattgc aagggatggt tatttttcta ccagtcggcc agcagctgat
     7801 cggtacagta agtttaacga tgacacaaca gttattgatt ggagcaaatg tgattcttct
     7861 attcttagtt gtagaaatag aaaaagggat catgaacaaa ctgtttcgca aaaaagaaaa
     7921 aacagctaga gaatacagtc gctagccgtt actataaaaa agttttgaaa gtgagttcag
     7981 tcaaacgatt gagctcattt ttcgttggaa tagaagaaat gtatacaata gggatatcta
     8041 gagaatctag agggaaattg gaaatcaaga tatctgtttt ttcaaaagaa atagtggaaa
     8101 gatcggttag ttcgataggt aagaagcaga cacgttttcc taagtaatct tttaattcct
     8161 gctgcaagaa cgccttccat gaaggttctc cttggaaaat aaaatagaca ttgaacaaat
     8221 tcggtttgat agaaaaaatc gtttgttgga tcagtagaga caagagttgg agaaagtaat
     8281 ttggttcatt gatttctatt tcttttttta gatcgttcag taaattctct ttctgttgat
     8341 acaagtgtgc atattgctga tcatatgttt ctaaaaaacg atggtattga cggtccaatg
     8401 cttcaatcaa tatcggcgat tcatgatatt ttaatatgag gagagccaag ttatcagtca
     8461 gagatgtttt ttttagttga tcaagagaca gctctgaagc tagttcctct acaaacgttt
     8521 cgaggacctc taaaaatgga gaatgatgca ggcagcgagc ggtttcttgt ccatagttat
     8581 tgttgtaatt ccaactttcc agaaagcaga aagaagcaaa agaaagctca gtgtctttaa
     8641 gataaatacc ttccgttaaa tacaattttt tcaatgcaga gtcatacagg gaggagagtg
     8701 tgctgttggt atggatagaa gaatctaatc ggcaattcgc ttttatacgg atcgtattga
     8761 taaagaacca acatgttccg aaaatctctt ctgtttcaac cgctagttca gtttgactga
     8821 ggtttcttaa aaattgaaaa taatgggatt catggatcga ataatcctcg aaaaaatagt
     8881 tgttatgagt gaaaggaatc agcaatcgat gataaaaagt acgaatcgca cgctcttttc
     8941 caagaataca agctgtatat ttcgtcaaat cgatctgcaa gtcgtaactt gctaaacgct
     9001 gattgatttt agtcacatgt cttttcaatg tcccataaga aagcccatgg tcggctagaa
     9061 aatcagggac agcaactttt tttgtaaaaa gcagattttt cagcagttcg acactgactg
     9121 attgtttgaa aaaaatctcc caaaggtcat ttgttaataa attttgctgg ttttgcaaac
     9181 tgattcctgc ttgatcgctg atgatgtccc agccttcggg cagctgagag cgaatagatt
     9241 gaaggtcaga aaaaatcgtt cgttcggttg tccctatgat ttcagctaat tcatttacgg
     9301 ttatctttgt atgattcagc aattgttcta ctaaagaaac atgacgtcga atatcttttt
     9361 cggtaatgat ttttttcatt agtgaataca tcaatcagac tcccttctat tctaagaata
     9421 cggagaaaat aa
//
LOCUS       DAHHZJ010000116         9231 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.117, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000116 DAHHZJ010000000
VERSION     DAHHZJ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.117"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            62..964
                     /gene="gnd"
                     /locus_tag="NBL02_002099"
     CDS             62..964
                     /gene="gnd"
                     /locus_tag="NBL02_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HCD2572776.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            966..1967
                     /locus_tag="NBL02_002100"
     CDS             966..1967
                     /locus_tag="NBL02_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HCD2572777.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            1964..2248
                     /locus_tag="NBL02_002101"
     CDS             1964..2248
                     /locus_tag="NBL02_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCD2572778.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            2281..3078
                     /locus_tag="NBL02_002102"
     CDS             2281..3078
                     /locus_tag="NBL02_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HCD2572779.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            3092..3925
                     /locus_tag="NBL02_002103"
     CDS             3092..3925
                     /locus_tag="NBL02_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCD2572780.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            3943..4800
                     /locus_tag="NBL02_002104"
     CDS             3943..4800
                     /locus_tag="NBL02_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCD2572781.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            4822..5331
                     /locus_tag="NBL02_002105"
     CDS             4822..5331
                     /locus_tag="NBL02_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCD2572782.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            5368..5769
                     /locus_tag="NBL02_002106"
     CDS             5368..5769
                     /locus_tag="NBL02_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572783.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            5881..6234
                     /locus_tag="NBL02_002107"
     CDS             5881..6234
                     /locus_tag="NBL02_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572784.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            6268..6711
                     /locus_tag="NBL02_002108"
     CDS             6268..6711
                     /locus_tag="NBL02_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCD2572785.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            6728..7813
                     /gene="uxuA"
                     /locus_tag="NBL02_002109"
     CDS             6728..7813
                     /gene="uxuA"
                     /locus_tag="NBL02_002109"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCD2572786.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            7806..8519
                     /locus_tag="NBL02_002110"
     CDS             7806..8519
                     /locus_tag="NBL02_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCD2572787.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(8726..8875)
                     /locus_tag="NBL02_002111"
     CDS             complement(8726..8875)
                     /locus_tag="NBL02_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572788.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
BASE COUNT         3099 a         1451 c         2052 g         2629 t
ORIGIN      
        1 aaaatctatt tacaaaaaaa tttttttgta taatgagggt ataagaaatg gaggcgctat
       61 catggaaatt ggtgtaattg gtttgggaaa aatgggattg aatttagctt taaacttaaa
      121 agagcaccat cacaaagtac aaggactcga catttcaagt acagctgtta gtcaggcaag
      181 agaaaatgac attcctgctt atcaagagat ctctgaattt ctagctactt ttgagcagaa
      241 aaaaatcatc tggttaatgc ttcctgctgg agagataacc gaaaacatgt taagaaaact
      301 ttttccaaaa ctcggaccag gagacattat tattgaaggc ggaaactctt attataaaga
      361 ttcaatccgt cgagcagaag agtttcagaa aaatgaaatc ggttacttgg attgtgggac
      421 ttctggagga atcgaaggag cacgacatca agcctgctta atgattggag gagataaaga
      481 aacttttcta tccgtggaac aattattcaa agatgtagca attgaaagag gatatctgta
      541 tgctggtgca tccggaagcg gacacttctt gaaaatgatc cataatggaa tcgaatatgg
      601 aatgatgcaa gcaatcggtg aaggattcca acttgtacaa aaaagtgagt atgcttttga
      661 cttggaacaa gtcgcacgtg tttggaatca tggttcggtg attcgcggct ggctgatgga
      721 aatcatggaa gatcagttaa acgaacatcc caatttaagt aattatcggg gaatcgtttc
      781 tgcatcaggt gaagcaaaat ggacggttga aacagcgcta gaaatggctg ttccagttcc
      841 agtaattgca actagtttgt tcatgaggaa tctttcacag gaagaagata gtttttcagc
      901 aaaagtagtt tcagcactaa gaaatgggtt tggcggacat gaaatagttg aaaagggtga
      961 gtaaaatgcc agattatcat atagcaatcg ttaattccag cagtttcgga agagtctttc
     1021 cacaccactt ggaaagatta aagtcagttg gagacgttca tttctttact gttgatcaag
     1081 agattccagg aaaagaacta gcggagttac ttaaaggatt caatatcatt attgcaagtg
     1141 ttacgccatt ttttcgacga gatttttttg aaaacaaaga tgaactgcta ttgatttccc
     1201 gacatggtat tggttacaac aatattgatt taaaagcagc aaaagaacac cagacaatcg
     1261 tatctatcat acctgcttta gttgaacgaa atgctgtagc agaaaataat ataactaatt
     1321 tgttgtccat gcttcgacgc acgacacaag cacaagcaag agtcaaggac gatcgatggg
     1381 aagatcgggc ggagttcgtc ggtaggacgc tgtttaacaa aacagttggt gtgattggtg
     1441 caggaaatac cggaagcgga gtaatcgaaa ttttgcgtaa cggttttcgc tgtgatgttc
     1501 tagcttatga tccttacaaa tcagatttat acatcgaaag ctttggtgca aaaaaagtag
     1561 aacttcaaga attactggaa caatcagata tcatttgttt atgtgcgaat ttgacagaag
     1621 agaactatca catgatttct gataaagaag tcgatcagat gaaagaaaat gtttatctat
     1681 ctaatagtgc aagaggcgct ctagttgatg aagaagcgat gattaatggt ctgaaaagcg
     1741 gaaagatcgc aggatatgcc acagatgttt tagaagaaga accgggacgt caagatcatc
     1801 cgtatttagc gtttgaacaa gtagtgatta cgccacatat atcggcctac acaagggaat
     1861 gtttagaagc aatgggtgaa aaatgtgtca gagatgtcga acagatcagt cagcaccttc
     1921 ttcctgaacg ttctgtccag acaaagagca actatataaa aggatgatga agaaaatggt
     1981 gaacggttta aaagtgaaaa caggccctca attttattta tatgaagaag gtggtatttc
     2041 aaaagtttct gatctcttga aatcatacgg cgcgaaaaga gtactggtca cccacggaac
     2101 ggtatcatgg gaaaaggcgc tacctaaatt agtatttttg aatgacgaaa cgattcaatt
     2161 tttttatcat cgttattctg gagaatgtag ttatgctgaa gccagacgaa tagctacaat
     2221 catcaaaaaa atgaaatcga tttcgtagtt ggcgttggcg gaggaaaact gaccgatcta
     2281 gtgggttata gtgccaatct tgcaagaatc gatttcggag tcatccctac tttagctagt
     2341 aattgtgctc catggacacc tcttgctgtc atgtataagg agaatggaca agcagaaggg
     2401 aaaacagaac acttttacag acaagctgct ttttttataa cagatcccca attagtgatt
     2461 gattcccctg ttccatattt cattgccggg ttagcagata cactagcaaa atggtatgaa
     2521 tctgagctca tcttagaaca agattttttg caagatgaat catttttgaa gctagcacaa
     2581 gatactgcga agatctgcaa agaagagatt ttgaatcatt ctgctaaagc aatccaagat
     2641 atggcacaaa gaaaactgac atcggagttc aaacagcttt cagagattat ctttgctgtt
     2701 gctggtttag ttggaggatt aggagataaa tacgcaagaa atgtggcagc acatgctatg
     2761 catgacgcta tcagtaaata tattccggaa agccatcgtt ttctacatgg agaaaaagta
     2821 gcgtatggga ttttctatca actggcacta gaaggtcgct gggaaaaaat agacgagtta
     2881 tatcctttgt atcgggaatt agaactgccg gtttctctag aacaaatggg attatttccg
     2941 aaagataaac agataataga taaaatggct gaatttattg attcgaaaga gaaagtgcat
     3001 ttgattccta tagaggtaac acagaaatca ctgatacagt caatgatgga actggaaggt
     3061 tatatcagaa agatctgaag gaggaggaca catggaaaca ttaagtgtag tacaaagttt
     3121 gttgattgct ttatgggtag ccgcggttat gtcccgctgg ttaggtggag gagccacgtt
     3181 gaccttacgt ttttctcctt tgatgactgg attagtcgtc gggttgatca tgggggaagt
     3241 tgcacaagcg atgattgtta cagccgcttt gcaaatgatt tatatggggg ttttttctcc
     3301 aggaggatca atgccagctg aaccatctat tgcagcagct atcgcagtac cagtcgcatt
     3361 attaggggat ctgtcgccag aagcagcgat tgccgttgcg gtgccagtcg gattgctcgg
     3421 aagttacttg tatcagtttc gttttttcat caatactttt ttaggtaaat atacagacaa
     3481 agctgtagaa gatttgaata caaaagctat ggcccgatca attattcttt atccaacggt
     3541 tgcctcattt ttattattcg ttccgcttgt tttcattgct ttatatttag gggcgccatt
     3601 gatcgctgat gtaatcaaag ctttagaggg aacagtcgtt atccacattc ttgaagtagt
     3661 aggtggaggg ttagcggcaa tcggtatcgc gacaactgta tacgttatcg gtcgaaaaga
     3721 ttatctggtc tttttcttcc ttgcttattt tatgagtgtt gttttgaaat ctttagatat
     3781 tacgatggtt acttatgcca tttttgggat tattatcgct ttgatctttg tccagtcgca
     3841 aaaagggaaa caagtacagc ctgccacaag caataacaat tcagcatctt ttgatgacga
     3901 agatgactat gatgacggat tttaatttag gaggaatggg aaatggctga aataatggat
     3961 aatcaaacag gtgctcctga agaaatcacg aaaaaagatg taacaaaagc ctatctgaga
     4021 tggcattttg caaacgaaat tcctcactct tttgaacgtt acttggcgcc atcactacta
     4081 tacggaatga tgccaatctt gaaaaaactt tataaagatg aagactcttt aaaagcagca
     4141 tataaacggc agttactgtt cttcaacact caattaagct ggggtggcgg tgtgattact
     4201 ggcctaatgt cttctatgga acagcaaaga gcagaagaag agtatgaaga aaaagagatc
     4261 ttgatgcagg atgacttgat gtataacacg aaagcgggat tgatgggggc actagcagga
     4321 atcggagatg caatcgattc aggaaccgtc caatatattt ttatcgcaat cgcggtacct
     4381 tgggcacaac agggaagtcc tttaggcgca ctttttcctt ttatctgctt tgggttgtat
     4441 caggttttac taggggtatt tttcgcaaga caagctttca ctatgggccg taatgctaca
     4501 ggcctaaagc aaaacactgg tgttcagaca gcgattgaaa tgctatcagt tttagggttg
     4561 tttatgatgg gggtgcttgc gggaaactat gtaaaagtag aatcttcatt gaaatttgcg
     4621 atatccggac gtgagtttgt tatccaggaa attcttgatc aaatcgtgcc agggatatta
     4681 cctttagctg tcgtaatggg tgtgtacttg ttctatacga aaaaaggatt gaaagtcaca
     4741 caagcattat tatggctgac tggaatatta attgtgctag caggaatcgg tattttataa
     4801 aaagaaggga acgtgaataa gatgggtgta gtcaatttag cgcgtgtgga tgagcgtttg
     4861 atccatgggc aagtaatggt aacgttatct caaaaaagtg gagtgaattc catttttgtt
     4921 gtagacgaag ttgtagcaaa agataaattt atgagagatc tttacaaaaa tgcaggaagc
     4981 cgtacaggac aaaaaacaat tgtcattaca ccagagaaag ctaaattcta ttgggatgaa
     5041 tatcaattta aagaatacaa ttgtattttg atcgcaaaaa cagtccaaac gatttacgac
     5101 ttagtaaaac acggaatccc catggaagaa ttgaatattg gagggattgc tcaaaaaaat
     5161 cctgatacag atcttttagt gacgaaatct gtttatttaa ataaatcaga tgctgaaaaa
     5221 ttgaaagaaa tgaatgaaca atatggcgta aagaatattt attttcaagc cactccatct
     5281 gcacctaaaa caggattaaa agatgtattg aacaagtttg atttgtcata aacaaatgct
     5341 tagaaaaaag ataaagaggg cgaaaaaatg aagaagaacg agctgtttcg tgattgggaa
     5401 tttcgttatc gctatattta ccgtaagcgc cgtacaaaaa agagtaaaca gcgttttctt
     5461 tcagcattag tatcagatat ttacagtatg agaacagatg tgacagtcat tgcttacgat
     5521 acgctagcct accgatcaaa aaatatttat gttggagata tcgaaaaagc agagaaagtt
     5581 atctgtactt attacgatac acccgttcat gcattaggaa gttatttcat gtttgattgg
     5641 aaagaccaaa ggaaaaaaac aatctactct atattattgt ccttcatttt gttgttcagt
     5701 ttaggttggt ggggaatgat gatatataac aaaaatcctc atcacgtatt cgatctgcta
     5761 tccgtataaa caggtatcac tgtactagca tttgggagct actttttctt attgggaaaa
     5821 gcagctcgcg gatggcctag cagacaaact tttattcgaa atacttcatc catattaacg
     5881 atgctagaaa tgatccgtac gattgatgat ccgagtgtag cgtatgcgtt tgttgatgaa
     5941 ggctgttatg gagaaaaggg cttagacagc gtgagatcgg gtatgaaaaa agaggctatt
     6001 cttttttatc tagactctgt tggggcagat acaccgctac agttttcggg aaattatttt
     6061 tcaaataaag aacagtggct gaaacaagtt gataagttga aagaaaaaaa cgtcaattat
     6121 atttttagtg caagaaaaaa gcaagcgcag ttcttctatt tgaccaaaac agatttacga
     6181 gggaaaacat ttaattggca aaatgccaat cagattattg ctctttttcg ataagtaaaa
     6241 agtgcaaaac aaggagtgag aagactaatg ttggaaatca tgatcgttac acatggtaag
     6301 ttgagcgaag gactgaaaga tgcggcggaa gtaattgtcg ggacatcgag ccatattgaa
     6361 acaatgtgct tagagtctgg ggaggacatc caaacattcg gagagaaggt caaagaaaaa
     6421 ttaactatga tcaacacagc agaacccatg ctagtatttg ttgatctaat tagcgcaagt
     6481 ccgtacaatc aagcgctctt ggcagtcaat gaactgactg aagaaaagaa agcagccact
     6541 tatgtcatag ggggcgtgaa tctgccaatg ttgttagaag caatcaatca tcagatgctt
     6601 cacacaccga tagaagaagc ggtggaacaa attgtcaaac aaggaaaaga taatttaact
     6661 gtttggcatg cttcagcgga agaaaaaaaa ttagatgagg acgattttta gaaggaaggc
     6721 tgaaaagatg aagtggggat ttagatggta tggtgaaaaa ggggattcga tcccattaaa
     6781 atatgttcgc caaataccgg gaataaatgg gataattgga actttgctga ctaaaatgcc
     6841 tggagatgtt tgggagattt cagaaattga ggagttgaaa gcatctgttg agagccaagg
     6901 actggaactg cttggaattg aaagtgtagc gatccacgat gcaatcaagg cagggacaga
     6961 tgaacgagat tactatattg ataactatat acgaacaatc aaaaacttgt ctcgatgtgg
     7021 ggttcgattg atttgttata gcttcaaacc aatttttgga tgggcgaaaa ctgatttgtt
     7081 taatgaaaac aacgatggaa gctattctct tttatacgat cagaaaatcg tcgatcagat
     7141 ggagccagga gaaatgtatc aattgataca cagccagtca aagggattca aattgccggg
     7201 ttgggaagaa gaacggttga aaaaattcgc tcagctggaa aaactgtatg aaggaatgac
     7261 cgaagaagaa ttatttgaaa atctgactta ttttttagaa aaagtgattc cagtgtgcga
     7321 aaaagcagat gtaaaaatgg ctatccatcc agatgatccg ccatgggaac tttttggttt
     7381 tcctagagtg acgaaaaatc ttggcgactt gaagaaaatt ctaaatgcag tagattctcc
     7441 atataatgga ctaacgctgt gcacaggatc tttaggggca aatccggaaa acgatttagt
     7501 tgaaatcatc catgaggtag gttcgcgcat ccattttgtc cacttcagaa acgtggagtt
     7561 tttaggagaa agaaagttca aagaaacggc acatccctct acagaaggct cccttgatat
     7621 gtatcatatt atgaaagcgt tggtgcaaga agggttcgac ggtcctatac gccctgatca
     7681 tggtagaacc gtgtggggtg aagtggctat gcctggatat ggcctgtatg atcgtgcaat
     7741 gggactcact tacatgcaag gtctatatga ggcgataagt aaggaaaaac cactgggaga
     7801 gcattatgaa taaaatcaag ctcttcatct ttgatatgga tggcctttta ttagaaactg
     7861 gacgtttagc ttatcgtgct tatgtaaaat cagcacaaaa gtatgattat gaaatgcgta
     7921 aggaagtcta ttatttacta actggccaaa cagaaatggc gataagagaa caaatgggca
     7981 ttttgtacgg agaagatgtt ccatatatca aatggcgaga agcaatcaat caatataaag
     8041 aaaaaatcgt gaaagaagat aagagagtat atacgaaaaa aggagcagaa gaaattttat
     8101 cttttgcaaa agaaagagga atccatacaa tcgtggcttc gtctaataca cgggtaaaag
     8161 tagaaatgta tttaaaaatg gaaaatctat atgccttgtt tgatcaaatc atttcagggg
     8221 atgatgtaaa aaaaggaaag ccggaaccag agatcttttt gaaagcttgt tcaaaaatga
     8281 atattcctcc aagtgaagca cttgtatttg aagactcgat tgcagggatc gaagcagcaa
     8341 ggcgagcggg cattttgtct tttctcatca aagatcattt gatagggttg cccgatcata
     8401 atgggaagca taagttgaaa gtagatgtaa gtttatatga atccaataaa gcagactatg
     8461 aattttatga cctgcatcaa gcaaaacgat ttttagaaag taaagggttg tatctctaat
     8521 aaagaggact attttctagt cctctttatt tattgtcctg cacgactgag ggaacagatg
     8581 aaaaagaagt aattttttat tttgaagtat ctaccttatt tgtagtgaac aatttagaac
     8641 aaacacaaaa cgcatctact gttatctacc agtagatgcg tttaaagaga agaacgagaa
     8701 tcgtccatat gagggtttcc actccttatg cagatttttt taattcttgc actgcttgtt
     8761 cgatcgtaac acctgtcgct acatgattgg tattttcttt gtcaaaaacg acaaatagat
     8821 tcaaatctgt atccaaggtt actcgatatt gtatatcttt gttatagaat gtcatagcca
     8881 ttctcctttc tttaaatact tgctgaaagg caacgaacgc gttgataacg aaattccatt
     8941 tataagtata ccaccaattg aaaggttttc atagttaaga aattttaaag tcaaaagttg
     9001 cagataaaag caaataaaca agaaaacaaa aagtgaatca atcgcggtta ttcaactttt
     9061 tcttcgattc gaagacaata gaagacaatg aattttatat ttcattgcat tatccttgtg
     9121 ttattagaaa aagcatgata tattaaatga gtaatctatt ttgaaacgta atttgagcga
     9181 tatatctaca tagataaaac tccttttgcc tatcaaatat ttattgcaac g
//
LOCUS       DAHHZJ010000117         8968 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.118, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000117 DAHHZJ010000000
VERSION     DAHHZJ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.118"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            367..1416
                     /locus_tag="NBL02_002112"
     CDS             367..1416
                     /locus_tag="NBL02_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCD2572789.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            1413..2294
                     /locus_tag="NBL02_002113"
     CDS             1413..2294
                     /locus_tag="NBL02_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2572790.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            2297..3067
                     /locus_tag="NBL02_002114"
     CDS             2297..3067
                     /locus_tag="NBL02_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD2572791.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            3346..4200
                     /gene="aroE"
                     /locus_tag="NBL02_002115"
     CDS             3346..4200
                     /gene="aroE"
                     /locus_tag="NBL02_002115"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCD2572792.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            4378..5541
                     /locus_tag="NBL02_002116"
     CDS             4378..5541
                     /locus_tag="NBL02_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCD2572793.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            5658..6068
                     /locus_tag="NBL02_002117"
     CDS             5658..6068
                     /locus_tag="NBL02_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCD2572794.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6242..8470
                     /gene="topB"
                     /locus_tag="NBL02_002118"
     CDS             6242..8470
                     /gene="topB"
                     /locus_tag="NBL02_002118"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCD2572795.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            8641..8925
                     /locus_tag="NBL02_002119"
     CDS             8641..8925
                     /locus_tag="NBL02_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572796.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
BASE COUNT         3111 a         1531 c         1931 g         2395 t
ORIGIN      
        1 aataaaaaat taaaaaatat agttgacgaa attcaaaaat tcgattagac ttaaatcaat
       61 tcttaaggaa agggtgacct gtaatgaact tatcaaaacg tatttcagca gaaaacaaaa
      121 aaaattggcg cagctggcgt agataagttg tattaatggt gagactttcc catagataca
      181 actttttaga gtgcatttct aaagcttgta tctatgctag acatgaacat tgactgccgc
      241 atagattaga atctatgcgg ttttttgtgt aaagacaatg accgctttgg ccattgtctt
      301 tttttgtaga ctggatactt gcctgattat aataaataaa aagattgact ttgcaggagg
      361 aaaaaaatga aaaacaaacg attaataaca gttgtagtaa ttattttagt ttatttagca
      421 ggtgcattta tttatggaaa agcctcaggc gaaaaagaaa gtacttcgac gaaccagaca
      481 aaaaaaacaa cagttgttgg ggtattgcaa tacgtgagcc atccggcatt agatgagatt
      541 tatcgaggaa tcaaagatgg tctgaaagaa gaaggattaa aagaaggaga aaatcttgag
      601 atcaaatttc aaaatggaca agccgatcag agcaagctag ccacaatgag tcaacagcta
      661 gtacaatctg atccagatgc cttgattgga atcgcaacac ctgccgctca atcattagca
      721 aacacgactt catcgatccc agttgtgtta ggtgctgtaa cggatcctgt gggggctgga
      781 ttagtcgctt ctttagagaa acctggtggt aatgtaacag gtgtttctga tcagccgcct
      841 gttacttcac aaatcaagct tgcttctgat ttgttgccaa atgcaaaaaa agtcggaatc
      901 ttgtatgctt cttcagagga taattcaaaa taccaagtag cacaagcaga agaagctgca
      961 aaaaaagaag ggttagaatc agtaaaatat gcggtgcctt ccactaatga gattgcacaa
     1021 acggttcaag tgatgagccg acaagtcgat tttatctatg ttccattaga taataccatt
     1081 gccaatgcga tgcaaacagt ggtaaaagaa gcaaacaaag caaatatccc cgtcattcca
     1141 tctgtggata caatggtgga acaaggtgga ctagcaacaa tcgggatcaa tcaatatcaa
     1201 ttagggcttc aaagcggtaa gatggcggca gaacttgcga gtggaaaaga aaaaccggaa
     1261 acgacgcctg tttatacatt tgatcaaggg gatacagtga tcaatcaatc tcaagctgat
     1321 cgcttaggaa tcattgttcc ccaatcgatg aaggaaaaag ccaaaatcat tacagatgac
     1381 tcgcagcaag aaacatctaa ggaggatgac aaatgatcgt atcagctatt tcacaaggca
     1441 tgctttgggc aatattagga ttagggattt tcatgactta tcggatctta gactttcctg
     1501 atatgacgac agaagggtcc ttcccattag gtgctgctgt ctgtgtgaca gctattactc
     1561 atggggtagc gccgattgta gctacattat taggcgtagg tgctggcatg atagcaggac
     1621 tcgttacggg gttactttat acaaaaggaa agataccggt tattttagca gggattttag
     1681 tgatgtcagg attgaactca gttattttgt ttgtgatgcg aacaccaaat ctttctttat
     1741 tgaatcatcc gattctacaa gacagcttcc atcgctttgg gttacctgat tactttgaca
     1801 cagtccttct tggcttcatc tctttgggca tcttgattgc tgtactgcta ttcttcttca
     1861 atacagattt gggtcaaggc tatattgcga caggagacaa cgaaacgatg gctcggtcgt
     1921 tagggataaa aaccgatcgt atgaaaattt taggtttaac tttgtcgaac ggtgtcattg
     1981 cattatccgg tgcgctgatt gcccagaacg acggatatgc agatgtaaat aaagggattg
     2041 gcgtaatcgt catcggtctt gcttccatca tcatcggtga agtgatcttc caagaattga
     2101 cgctagcaga aagattgatt gctattgttg tcggcagtat catttatcag ctattgattt
     2161 tagtagtaat caaactaggg tttgacacga cttatctgaa gttgttctcc gctatcatct
     2221 tagctatttg tctgatgatc ccgcaattga agaaagcact gaacttaaaa acgggttttg
     2281 agaaggaggc atagtaatga ctgatatatt gacaatcaaa aatggcagaa aagtcgtaaa
     2341 caatggaatg aatgaagaga aagtactatt ggatgaaatt aaccttacaa tcagagaagg
     2401 ggacttcatc actgtccttg gcgggaatgg tgctggaaaa agcacactgt tcaatacgat
     2461 cgctggtacg cttcaactca gtcaaggatc gatctctttt aaagatcagc tgatcacaaa
     2521 agattcagaa gaaaaaagag cacgattcct ttctcgagtt ttccaagacc ccaagatggg
     2581 aacggctcct cggatgacag tagcagaaaa tttgctactt gcacaaaaaa gaggtcaaaa
     2641 acggaaattc cgtttacgta acttgaaaaa tcaaaaagaa gattttttca gactatgtca
     2701 agaagtcggt aatggcctag agcaacactt ggatactcca gcgggtgaat tatctggtgg
     2761 tcaacgccaa gcattaagct tgctgatggc gacgatcaca gctcctgagt tactattatt
     2821 agatgaacat actgcagctt tagatcctaa aacagctaaa gcattaatgc gattaacaaa
     2881 tcagcggatc aatgaacaaa aattgacttg tctcatgatc acgcatcgaa tggaagacgc
     2941 cttgaaatac ggaaatcgtc tgatcgtact ccaaaaaggg aaaatcgttc aagatatttc
     3001 tgaaaaagaa aagcaacatt tgacaatggg cgaccttttg cagttctttg aagaagtagt
     3061 ggattgattt cgacagaaaa attcctttca gtagaaggac ttgaaaactc attgacatct
     3121 tcctttgatt catcctatac ttaaaacaat aaacaattaa acatatttgg gtgttttgta
     3181 ggatcacgta gtcaatgatt ttaacaatta aatagaatat tctttgctct gacaagtaaa
     3241 cgatgagaaa agaatttgct actttgccac ctagacgttt cgtacttttt cgtatcaaac
     3301 gctaagtggc tttttttatt gaagtaagaa caaaggagga ataagatgat ttctggacaa
     3361 accaaattag ctgggctctt tgctgatccg gcttctcaca gtttatcccc attgatgcac
     3421 aatactgctt ttgaagcaaa caacatcgat gcggtgtatt tagctttcca agtaaatcca
     3481 atgaatttga agcaagcagt cgaaagtatt cgaacttttg atatgttagg tgtaaatctt
     3541 tcgatgccga ataaaacagc ggttattcct tatctggatg aggtaagcca ggaagcgcaa
     3601 ttgatcggag cagtgaatac gatcgtgcac gaaaagggtc gtttgattgg ctataatact
     3661 gatggtgcgg gcttcatgca atccgtgcaa gaagctggga tttcgatcaa aaagaaaaaa
     3721 atgactctac tgggagctgg aggtgcagca aaggcagtca ctgttcaagc agcgttagac
     3781 ggcgctgagg aaatcgtagt ttataaacga aacaaccaaa cattcgctca agtaaaagaa
     3841 acattcgaac aactcagcta tcgaacttct tgtaagataa aagttcgtga ctttgccgat
     3901 acgcatcaat taagaaaaga cttgaaagaa agctgtcttc tagtcaatgc aacagatatc
     3961 gggatgggaa agaaaatcaa tcagtcaccg ctcttagata taagcatgat gcatgagaat
     4021 ctagcggttt ttgatttgat ttatgcacct agagaaacac gtttattaaa agaagcaaaa
     4081 aaagtaggag caaaaggata caatggactt ggcatgttga tctaccaagg agcaatcgcg
     4141 tttgaattat ggacaaagga aaagatgccg atccatgtga ttgaaaagct gtttgattaa
     4201 aaaaggataa tagataaaga tcttttattc tcgcataaac ggtctcaaga aaagtaatcc
     4261 tttatgtatc gaagcattta tttcgttatt ttttattaaa ttatcaaaaa atgataggct
     4321 tttgtcgatt ggttatcgta tcctttattt atcagtaaga aacgaggagg aaccacaatg
     4381 agtcggatgg atcgctataa ggacatccat aagaaggcga aacctctgaa gcgggatacg
     4441 gggagtattt ttgctagaag agaacgaaaa cgaacgcgta tggaaaatga ggaagatgct
     4501 acccgtattt atgaaagaga gcagcattca tttgaacatt tgaaccaata cgagcaacag
     4561 atatacagag atgcaccaag agcagaagaa caacaaaaaa ataaaaaatt tttcaatcgg
     4621 caaaagaaga acaaaccgtt aaaacctcga agaaagcgat cgtggaaaaa aatcattcta
     4681 tcgattttgc ttgttttgat cctttattca ggtatctcat tttttattgg taaatatgta
     4741 gctgaacatg acacaagctt agaggcagcg acttcagaaa cctttcatgg gcaaaaagcg
     4801 acgaatggtg cgcataatat tttgatcctt ggaagtgata cacgcggaga agatgccggg
     4861 cgagccgata cgatcatggt cttacagttg gatggtcctt cccataagcc gaaactgatt
     4921 tcttttatgc gtgacagctt cgttacgatc ccaggggtag gacaaaataa gatcaattca
     4981 gcttatgcat acggaggagc cgatctagtg cgtcaaactt tagtagaaaa ttttggaatc
     5041 gattgtcaat actatgcgaa agtcgatttc aaatcgtttg aaaaagtgat cgatgcctta
     5101 ttcatgaacg gagtgaagat cgatgcagaa aaagatctga atctggatgg tgtagatatt
     5161 aaaaaagggg gccagaagat ggatggacat gttcttttgc agtacgcacg tttccgaatg
     5221 gatgaacaag gagactttgg ccgtgtcaga cgccaacagc aagtaatgaa cgctattttc
     5281 agccaattga agaatccatt gaatctgatc cgtgcacctt atgcagctgg taaagcttta
     5341 ggatatacat ccacagatgt cactgcttgg ttcttgacga aaaacctctt ttcgattgca
     5401 cgaggagctg gtggcattga tcgaataagt gtgccagtag acggttcgtg gaattatggg
     5461 caaagtgatt atgctggcag tattcttgaa attgataatc aaatgaatca atctgctatt
     5521 acagattttt tgaataaatg atcataaaaa agatcaagaa gaagctgcgc aaggcttttg
     5581 tcatgaatca atcaaacgat tcactcgtaa aaaacgcttt cttatggtag atttaaccaa
     5641 gagaggagct gtttacattg gatatctatc tgaacttcaa aaatgagtcg cgtgaagcca
     5701 tcgcttttta tgaaaaagtt tttcagactg aagcatcagg tatcaccaca tacggtgaaa
     5761 tgcctggaat agaacaaatg aacttgccag aagaagcaaa aaatctaatc acgaatgcac
     5821 ggctgaacat ttatgataca ttagttatgt ttgcagatac gccagaagga attggtcctg
     5881 aattagttgt gggaaataat gtttctttag tcatccagac aagaaatgaa gaagaaacga
     5941 agcgcctatt tgaaagctta gctgagggtg gtaagattga gatgccactg gatgctgttc
     6001 cgtgggctca gttgtatggg atggtaactg atcgatttgg catcaactgg cagattaacc
     6061 aagagtgaca gctaaagatg aatattttct ggaaacatca agcagcaaac ggatcatcgt
     6121 ttgtttgctt gatgtttttg tgtctatttc gactatacgg tatctttttt ttattgccag
     6181 atgcgaaaat atgttccata atagagaagt ccgcaaaaaa ttaagtaacg gaggtcagaa
     6241 gatgaagacg gtgatactag ctgaaaaacc ttcacaagca aaagcctatg ccgaaacctt
     6301 ccaacagcaa aagcggcatg aagggtatta tgaaatcagc gatccattgt ttgctggtga
     6361 agtaaccatc acttatggtt ttggacattt ggtagatatg gttgctccag gagcttatga
     6421 agagcgttgg gcaaaatggt ctttagaaaa tttgcctatt ttcccagaaa ccttccgtta
     6481 cgaagttcca atggacaaac gtgcacaatt ttcgatcgta aaacgagaat tgcagtcagc
     6541 cgataccatc attattgcaa cagatggtga tcgagaaggt gaagccatcg cgtggtcgat
     6601 tattcgacaa gcaaacgcat tttctaagga aaagaactat ttacgtttat ggatcaattc
     6661 tcttgaaaaa gaagcaatct atgaaggttt taagaatctc caacctggaa agaactattt
     6721 tccaaaatat aaagaagcgc aagcaaggca gaacgctgat tggctgattg gaatgaatgg
     6781 aagtccatta tatagcttgc tacttcaacg aaaagggatt gatggcagtt tttctcttgg
     6841 tcgtgtacaa acaccgacac tctatatgat ttatcagctg caagaagaaa tcaaacactt
     6901 caaaaaagaa acgtactttg aaggagaagg tttaattacg acaaacaaag ggcagttttc
     6961 tggaaaaatc gtaccaaaaa aagcatttaa aacccaagaa gagttaacga aggaaataga
     7021 aaaacttgga gcgcatttag gtaagcaaaa cggacggatt cttgaagtta cgaaaaagga
     7081 aaagaggttg catagtccac gtttattttc gctatctagt ttgcagacaa agatgaatca
     7141 gctaatgaaa gcaagtgcca aagctacact tgatgctgct caaggattgt acgaaagcaa
     7201 gtttctaagc tatccaagaa cggattcctt ttacattaca gaaaatgagc atcagtatct
     7261 ggttaaacat ctgcaaaatt ataaaaaatt tttaggaata gaagaagtac agacaactga
     7321 gatcaaagca aagaaacgat atgtcgatgc gaaaaaagtt caagaacatc atgcaattat
     7381 tccgacaaag acgattccga ctcctgcacg ctttgcgaag ctttccaaat tacagcaagc
     7441 aatctatttg caagttttgc gcactactgt ggctatgttt gctgcagatt atatctacga
     7501 agagacgacc gtaatgacgg gcgtacagca attaaagcta aaaagcatcg gaaagatccc
     7561 attacagcaa ggttggcagg aaatcttacg catcaagtca aaaaagaaag aagctcagac
     7621 tcttcctcct atcgtagaaa atgaagaagc agaagtagaa ctgaaaagta tggagaaaga
     7681 aacacaacct ccaaaaccct ataccgaagg aacgttgatc acagcaatga aaactgcggg
     7741 aaaaacgctg gataatgatg aagcgcaaga aattctcaaa gaagtagaag gaatagggac
     7801 agaagctaca cgagccaata tcattgaaaa tcttaagcag aagcattaca tcgaagtcaa
     7861 taaaaacgaa ataactgtta cgccaaaagg aatcactctt tgtaaggcag tagccaatga
     7921 accattactg acgagcgcag aaatgactgc gcgctgggaa gggtacttga gaaaaattgg
     7981 taaacaagag gggacaccat ctgttttttt agagaatatc aaaaaattca tcctacattt
     8041 attggcagaa gttcctaatc aaatcgacaa ggtagacttt tcagatgaga ttagcgggac
     8101 acgtcagata aaagcgatgg agaagaaaaa cgatcaatta ggaagatgtc caaaatgtaa
     8161 aaagggaata gtcatgcttt atcctaaaat cgctacatgc ttgaatccgg aatgcgactt
     8221 caaattgtgg ccaacagtag ctaaaaagaa gctgactaaa acgaatctca gagatttgct
     8281 ttcaaaagga aagacaagta aagttgtcaa aggttttact ggtaaaaaag ggaaattcga
     8341 tgcagtttta gtattaaaag aagatatgac aatcggtttt tcttttccat ggatcgaaaa
     8401 cagtggaaca aaagcagttg atggaaacaa agaaacatca agaaaaaata atgaaaacga
     8461 agtagagtaa atgaaaacaa tgaaatagcg gaatatttat tccgaaaagt gagaatataa
     8521 ataccaataa aactaaaaaa tatcagtgaa tcaagcaaaa acgttggggt aggctgattt
     8581 tatatagata gcaaggagtt tttttatttg aaaaaagtgc attcattttt aaattatctt
     8641 atgggagcaa tcacgattgt ggctgtgtac caagcatttc taaagtatcc tgattacggg
     8701 acgaagttgt tgtcattgac gcacaatcaa acagcatatt caccaacgtt gattttatcg
     8761 actttgactg caggagctaa aagcgctact tatttttttc ataaaacgac aagcatgcta
     8821 aagcgaaaga aacagcgaaa acaattgttg gtggtggtag cggctatgat cattatttat
     8881 tttctaccag ttattacaca agtatttcat caatattttg gttaagaata cgagagtttg
     8941 tgacaaaagt catgtcacaa actttttt
//
LOCUS       DAHHZJ010000118         8776 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.119, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000118 DAHHZJ010000000
VERSION     DAHHZJ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.119"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..289
                     /locus_tag="NBL02_002120"
     CDS             <1..289
                     /locus_tag="NBL02_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572797.1"
                     /translation="VEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKPVRTR
                     SAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(386..820)
                     /locus_tag="NBL02_002121"
     CDS             complement(386..820)
                     /locus_tag="NBL02_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD2572798.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1052..1762)
                     /locus_tag="NBL02_002122"
     CDS             complement(1052..1762)
                     /locus_tag="NBL02_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCD2572799.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1752..2612)
                     /locus_tag="NBL02_002123"
     CDS             complement(1752..2612)
                     /locus_tag="NBL02_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HCD2572800.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2617..3255)
                     /locus_tag="NBL02_002124"
     CDS             complement(2617..3255)
                     /locus_tag="NBL02_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCD2572801.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3270..3569)
                     /locus_tag="NBL02_002125"
     CDS             complement(3270..3569)
                     /locus_tag="NBL02_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCD2572802.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQTEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3612..5096)
                     /locus_tag="NBL02_002126"
     CDS             complement(3612..5096)
                     /locus_tag="NBL02_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCD2572803.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5116..5577)
                     /locus_tag="NBL02_002127"
     CDS             complement(5116..5577)
                     /locus_tag="NBL02_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCD2572804.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5595..6662)
                     /gene="ulaG"
                     /locus_tag="NBL02_002128"
     CDS             complement(5595..6662)
                     /gene="ulaG"
                     /locus_tag="NBL02_002128"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCD2572805.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6880..7650
                     /locus_tag="NBL02_002129"
     CDS             6880..7650
                     /locus_tag="NBL02_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCD2572806.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7923..8750)
                     /locus_tag="NBL02_002130"
     CDS             complement(7923..8750)
                     /locus_tag="NBL02_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD2572807.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2653 a         1850 c         1381 g         2892 t
ORIGIN      
        1 tgttgaattt gacagtgaac tacttaagca atttccacaa gaagaacatt tcatgtataa
       61 agaagcaatt tcccacaaag tgacagagta tttggagcat aatttttttc gtataaagcc
      121 agtgcgtact cgttcggcaa aaggaatata cgagatgaaa gtcaaaatta ataaaaaaaa
      181 ttatcgcata gcatttgctt taaaaaaaaa tcatgtgttc attttttata ttagttcgaa
      241 tttgcaaaaa attgtatttg ataaagaggt gagtaaaaaa ataaaatagc tgagcattgg
      301 aggatagatt gagtgatact aagtctatcc tcttgttttt ctaaagaact aagctagaat
      361 tttttctttt gtacgttatt atatgttatt tgtcattacc aaaaagcacc ttttttcttc
      421 tagtatctat gacataagaa ataccaccca tttcctttac ttgaacaaat cctactgatt
      481 cataaaattt ttttgttttc tcagtatcat ctgtcaataa ttcgatttgg actggtccat
      541 aactagtgat cttctccaaa gcttcattca aaagcacttt gcctattcct tggcgctgat
      601 acctcggatg aatgaggata tcttgaataa atgcgatcgt cgcaaaatca gtgatccaac
      661 gaacgattcc gataatcttc ccttccccat tacgctttat cagagattca gattgttcaa
      721 atgctctagc taaccttgca gtgtcttttg tgtaattcgt ccaaccaacg ctcttatata
      781 attcagaaat ctcatcgatc ataagggaat caatattcat ttttttcttc ctttcttata
      841 caattctccc tctaataata cagttctttg actatttaat ctatcacata taactctcct
      901 gtaaagattc tctcgattcc ttaaaattcc tctttccttt tagaatgcat actttttgat
      961 aaataaaaag gttgtgataa attttcttca caacctctgt ttctctctat caagtgttat
     1021 cttttaattt tctaccctta ccctagcatc tctattgccc gtagtatgca tactttccat
     1081 gtttgcgaaa ataatgttta tctagtaaat attgcgggat ttcacatata ttggggttga
     1141 tcagctcgtt ttctttggcc atcagacaaa tttcatctaa gatcaaactg ttttctactg
     1201 ctttcatagg tgaatccccc catgtgaacg gtccatgtcc atagacaagt actccaggga
     1261 cttcgtttgg atctaatttt ctttccttaa acgtctcgac aatcacattc cctgtatgaa
     1321 cttcgtaagc ttctcttact tcttctttcg ttaattggcg tgtacaagga accttaccat
     1381 aaaaagcatc agcatgagtc gtgccataag ctggcaaatc gcgtcctgct tgcgcccaca
     1441 tgacggagtg cgtggaatga gtatgagtaa tagcagttat atcttcaaaa gtttgataca
     1501 aaacaacatg tgtggggaga tctgaagatg gttttaagct atcttcttct attatatttc
     1561 ccgacaaatc tgttaccacc atttgatctg cttgcatgca ttcatatctg acaccgcttg
     1621 gcttaatgac gatgactcct agctcacggt ccacttcact cacattgccc caagtcaatt
     1681 tgactaatcc gtatcgcggc aatgccagat ttgcttcaag gacacgttgc ttcattgctg
     1741 caattttttc ttcacaagac atcatagcca gcctccttca atttagaact taagtagtct
     1801 tttgcctgct ggatttctct tcgaaagtct tggctttttt cactccacat ttcaatcaga
     1861 aatgttccat cataatctag acgtttcaaa tgtcttaata atccaagaaa atcaacacaa
     1921 ccgctaccaa aaggaacatc acgaaactgg cctgaatatt cttttgttac agggtaagta
     1981 tcttttaagt ggatagcagt gatataatcg atccctttct caagttctcg agccggatta
     2041 ttttccggcc atgcggacaa attgcctagg tctggatata cttgtagaaa aggtgaatga
     2101 atctgttctt tgatttctaa aaattttgaa atggagttca taaatggatc atccatgatt
     2161 tccacggaaa gaatgatgcc atatttagaa gcttccttta cgctttcttt tattccttcg
     2221 ataaagtctt ctctggaatt cattgatttc tcttcataat aaacatcgta tccagctatt
     2281 tggattactt tgacgcttag attatgcgcc aaatggattg cttttttcat tagttgtttc
     2341 gctgcttttt tcttttctgg atcagcagaa ccaaacggaa atcgccgatg cgcacttaaa
     2401 caaatcgaat ggatacgtac accggagctg aacatctctt ctctcagctc agctatcttt
     2461 tcttcagacc aatctaatcg tgctaaccgc tcatccgtct catcaatcga catctcgata
     2521 aaatcgaagt ccatttcttt tacaagggaa aaacgttctt tccatgaaat atctttgggt
     2581 aacgcttttt cataaatccc aatttgagcc atttccttac ccccagatac gaatgatctc
     2641 atctttgaag ttttgtgcgg ccttcatcgg atcatttgct gctgtaatac cacgaccagt
     2701 gataaaggta tacacatcga tcccttcaaa taagtgtaat gtgtgtggat ttaatccacc
     2761 agttacagaa acattaaagc ctagttcaat cagttttttg atcttcgata aatctttttc
     2821 accccaagtt tcaccagata acagcgcatc acggctttga tggtagatta cctgccggat
     2881 accgatgtta tgccaatcca tggcttgttc aaaagtccaa ttgccgtaga gttctacttg
     2941 caattcacct atttcttttt gtgctgcctc catagttgga atcgttgcac aacaaatgac
     3001 tgtcatccag tctgctcctg cttttgcaac attactcgcc actgttcctc cagcgtctgc
     3061 acatttcgta tcagcgacta gttttttatc tgggaacata ctgcgaatac agcgaatcgc
     3121 tttttgtcct tcctgtaagc aaagaattgt gcccacttca ataatatcta ctacatcacc
     3181 aactttcata acatctccta atgcatgttc taagtcattg tgatctaacg caatttgcaa
     3241 attcggcctg ctcatcttag ttccttcctt tacttgtttt tttggtacca ctttgtttca
     3301 gcaaattttt cactgatttc tttgactgac atcacatttt tgataccgat aactgtaact
     3361 ccttttttct ctgtctgagt aaacatattc aaaaaattga ctggcgtaaa aactacatcg
     3421 tattggccag ctgaactttt tccctctgaa atggcacaat gatggatttt ggtaattggt
     3481 acgtttagtt ctttcattgc tttttccaca cttctcatca tcatcaagct tgttcctgat
     3541 ccatttgcac acgatactaa aattctcatc ttaatccctc cattatgagt gttgtctaat
     3601 cagattttct tttatactct ctgcttttct gcatatgtgt catagtcttc tacaatcaaa
     3661 aaataaccat caggattttt ccgatattgt aactgcggaa tagccaataa accaatgaca
     3721 actacggcaa ttccagcata acctaggact ttcatgatta ctgtaaacaa tggccaagct
     3781 gttgcccagt cgaacatccc gatataacca ccgtatgttg acatcccaat ccatgtagcg
     3841 aataatgctg aaccgcctac ttgtatgatt cctgaaagaa acgggaacag gcaagcggct
     3901 ttgaatccac ctcggttatt ggcataaact gctataacgg cattatcaaa gaacaacgga
     3961 atgaaaccgg ctatgacgat cgttggtgat ttcaataaaa tcagtaaacc aatcatcaaa
     4021 aactgtccca aagcaccaaa caagaaccca atcgttaccg catttggcga accaaagcca
     4081 aaagtagcag ctacgtcaat tcctggaaca gctcctggta aaatcgtgtt tgagattcct
     4141 tggaaagatt gagttagttc atcgacgaat gtccgaacac ctaattgcaa gatcgccaga
     4201 tataccgcaa aatttaaaga agtcgtcata atgtagaata agaaactctg cccttcttgc
     4261 atgaattgcg cttgaatcaa ataatctttg cctagtacaa tcagaatgat cccaaagaaa
     4321 aatagcatga gtaacgaagt agcaaccata ttctcattga aaatcgacaa gaatcctggt
     4381 aatttgatat cttcaatttt tttgtcttgc ttgattgatc tatttttctt ttcatcccgc
     4441 tttttcatcc actcagctaa ttttgcaaat ataaagattc cgaacatttg ctgatgtgcc
     4501 actgcaaatc cggcaccttc tgtcaattcc tgcgtgattc ctactgttaa gttagaagct
     4561 actgtccaat aacaacctaa aatcagtccc ataaaaatca atgcttgtat cctacctaga
     4621 tcaggaaagc aaaacagtaa aatccaaaaa gcggtagctg cttgttgaac ctgaacgttt
     4681 ccggtagtga aaacggaacg cagtttcgta tatttctgaa aacggactaa taagatattt
     4741 aaaacaaatg caatcagtaa taaaatcatc acatctccaa aggtacggcc aaatgtctcc
     4801 attaatccag catccacagc attttgtcca aaataaggat ctgtaaccat ggcatccaaa
     4861 ttgaaccgtt cctttagtcc aaccaagatt ggccgaaaat tattgaccaa accaccagag
     4921 ccaacagtca aaataaagta accgaccgtt gcctttaaga atccagcgat gcattcatga
     4981 agcgggcgtt tcagcaaaat atagcctaat aacacgataa aaccaatcag gtatgctggc
     5041 tgcgtcaaga tatttgttgc aaaatacgtc caaatgctta acaaaaattc ccccatcttt
     5101 tttctcctct atcaattaat tatattctgc catcacaact tggtaatcat cgatggagtt
     5161 cgtagagatg agacgctcaa ccaatccatc tgtcattaat aattcggata atttttggat
     5221 attttctaga tgttcttctg gatcttttgc tgcgagtgta aaaaacaaac tagccgtttt
     5281 ctcctcattc tccccttcac tttcaaaacg gattggctgt ttcaacttgg taaatgaaat
     5341 agctgttcca aaaacaccat cacttttttc tgaagaatgc ggcattgcta cttgaggtgc
     5401 gatcacaata taaggaccgt atttttctac acaatggatg atctcctcca catacgtttc
     5461 atcaataatt tccttatcga tcaatacaag acagctttcg gcgatagctt cacgccaatc
     5521 cgtgatttct ttatctgggt aatgaatcaa ttgatggtca aaaaagtatt tcagcaagaa
     5581 actccccttc tttcttataa aaacgacgga aacggtgtat cattttcaat cgagaagaca
     5641 tcttcaaatc ctcgataaaa attaaactca agatcatctt tgtttgtcgg atagatgaat
     5701 ttcccgccta cttgccagat ataaggttta aatccatact tcaggcgatc ctttttgaat
     5761 ttccaaattt cagtgatctc ttttggatca gccataaaat tagaccaaat atcgtagtgg
     5821 acaggaatca ctacttttgc attcaatgac tccgccatgc gcagcatatc aacagaagta
     5881 actttgtctg ttatccctcg tgggttttca ccataggccc ctaaacagac atcgattttg
     5941 tgttcattac catgtttggc aaataaattg gagtaatgtg aatctcctgc atgataaaga
     6001 ttccctcctg atgtttcgaa taaatagttt actgcaattt gatccatctc ctgaggcatt
     6061 tttcctttta atacctcttc tggatcttca caagtgacca acgctgtccg atcaaacgct
     6121 tctaaagcta ctacattgat gtctttgatt ttgactgcat cacctggctt tactgtaatc
     6181 gttttttctt caggaattcc ccatttttgc cacgtgcgaa ccacttcttt tggcccaatg
     6241 aatgttgctt caggacaatt ttgatgaact gctgcggctg tatggatatc tagatgatcg
     6301 gaatggatat gtgtaacgat taaagcgtct acatttttta tcgcaaaagg atcgattacg
     6361 aaaggctgag tacgtaaatt aggctgcata ttttgaacgc cactcatacg catcatctga
     6421 tggccttttt tcatcatgcc attcccatga ctacgttttc ccgtcccaca ccatagatca
     6481 cataaaatat tcgttccttc atgacttttc agccaaattc ccgtacagcc cagccaccac
     6541 atagcaaaag ttccaggatc cactacttct gtttcgattt cttcattcaa gtatgtgccc
     6601 cactcaggaa atgtttctac taaccacttt tcttttgtta cttcattgac agttttactc
     6661 atttgcttgc tccccccata tcgttgttcc aacttacaag taaatattaa tgtgtatcag
     6721 catccatgaa aaccctttta tatgaccgtt tgttttttat atgattttct ttcagtaatc
     6781 aatgattgtt tcagtataat aaagacagat atgaatgttt aaatgaacaa ctagtcaaat
     6841 aaatccatgg atcaagatgt ccctcaggag gcaaaagtca tgaaaaactc acttgtcaat
     6901 attgaaaaac gtcaaaaaga aattttggaa ctgcttcagc agactgaaca actatcaact
     6961 ttcgaattag ctgaatccct tgatgtatct attagtacga tacgaagaga tttaaattta
     7021 ttagaagaaa aaaacgagat catacgcaat tacggttatt gttcttataa ttacaaaaac
     7081 cagactgatt ttgatcaatc tggtcctgaa aggattaaac aagcgattgc cagagaagct
     7141 agtaaataca ttaacgatta tgatacgctg tttatcaatt ctagctcgac tgcgttgaaa
     7201 acactggatt ttcttcaatc taaccatttg acagtcatta caaacaacgt gaaaatcaat
     7261 tactctccgc acaaagataa ttgtagttat attttaactg gtggagagat gcgctttcca
     7321 aaagaagcac tagtgggcga catcgcatta aatacgattt cttcagtcaa tgcagacatt
     7381 tgtatcattg gctgtagcgg agtcgatgta gtacatgggg tcacaacaaa gatattaaat
     7441 gaatcaacga tcaacgaaat gatggtcaac aagacaatca aatgtaagat tttagtcgct
     7501 gatcatcgaa aaatcggact gacatcaaag ttcaagattg ctgatatttc agtctttgat
     7561 tatttgatca ctgataaatt ttgttctgaa aaagtattag aggagatcag aaatacgggg
     7621 ataaaaacca ttcaagtaga ttcattttaa gaaccaggac gtaaatatgt aaataggatt
     7681 atcaaaaaaa tgaataacaa atttatcgat aaatgaaaaa cacgctacgc tcaaattgat
     7741 aaaggaatcc caagatgtat ccatccaatc gataccatca taatctattg cttcttttat
     7801 aaccgttata tgttgcctaa ataggaatca aacaatatcg aaaaaaggag gtatgacaaa
     7861 agtcacacct ccttttttcg aataaacggt attcaaatca gaacactgat ttcacaacca
     7921 ctttatttca ggtcgttttc aatcaccttt accgcgtaat catttccgtt gaaacgcgct
     7981 attttttttg cgtccatata tctttctaaa taatcatctt ctcctaattg cttatggcaa
     8041 gaagcaattt cataaagcaa gtattccaat gcataagtcg atttatgttt cttattatac
     8101 tcaataccct tctcacataa ttggattgct ttatcataat cctcgatttt acggtaatag
     8161 gtggcgatat cataatatac aatgcagacc atcagattat caaaatcagt ctcactcaat
     8221 attttaagtg tacgctccac ataaactttc gcaaaatcta attctccttt tgacaagtag
     8281 atcattgcca cgtttgcagc tgcttgtaag tgatggatat ctgtttcggt cgtttgttcc
     8341 aacacttgat taaaataaaa taatgctgca tctggttttt tcgcataaag atgatcaagc
     8401 agtccttgat aaaataaaat ttgatttaaa agatcagaat tacttacttt ttcctgatcg
     8461 atcgtttgta aaattttata agattcttgg tattttgata cagaattcaa tgattgaatc
     8521 tcatttattt ttttcctgat tttgtcttct tctgtttcta ctaatatatc ggagatctcg
     8581 acattcagct ttttgcaaag cgcacttaag atattgatgc ctggcgacat attcttgttt
     8641 tcgatattgc tgacagttgc ttgttcacaa atgccttctg ctaattgtac ttgtgttaac
     8701 ccctttttct ttcggtattc ttttagtaat gctccatcta ctttaatcaa aacaatttcc
     8761 ttccctttaa aaaatt
//
LOCUS       DAHHZJ010000119         8662 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.120, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000119 DAHHZJ010000000
VERSION     DAHHZJ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.120"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(78..410)
                     /locus_tag="NBL02_002131"
     CDS             complement(78..410)
                     /locus_tag="NBL02_002131"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572808.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(424..681)
                     /locus_tag="NBL02_002132"
     CDS             complement(424..681)
                     /locus_tag="NBL02_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572809.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(876..1418)
                     /locus_tag="NBL02_002133"
     CDS             complement(876..1418)
                     /locus_tag="NBL02_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572810.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(1439..2530)
                     /locus_tag="NBL02_002134"
     CDS             complement(1439..2530)
                     /locus_tag="NBL02_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HCD2572811.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(2538..4748)
                     /locus_tag="NBL02_002135"
     CDS             complement(2538..4748)
                     /locus_tag="NBL02_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572812.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(4764..7292)
                     /locus_tag="NBL02_002136"
     CDS             complement(4764..7292)
                     /locus_tag="NBL02_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCD2572813.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(7344..7754)
                     /locus_tag="NBL02_002137"
     CDS             complement(7344..7754)
                     /locus_tag="NBL02_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572814.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(7767..7985)
                     /locus_tag="NBL02_002138"
     CDS             complement(7767..7985)
                     /locus_tag="NBL02_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572815.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(7982..>8662)
                     /locus_tag="NBL02_002139"
     CDS             complement(7982..>8662)
                     /locus_tag="NBL02_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCD2572816.1"
                     /translation="EQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETI
                     PTASYKVTYDIRSMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEK
                     QRMITYDDTLYETVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVD
                     QVEMKVLRYKETKKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWL
                     DRKGEERK"
BASE COUNT         2622 a         1873 c         1267 g         2900 t
ORIGIN      
        1 taaaacacca aatattttaa ttattctttt cataatttct cctccataaa aaataagtta
       61 ctttttaaat atcttgttta tgaattcatt ccccaaaggc cttttaagat tgatacagca
      121 gtccgttcac ttcttttaga cttgtcatac aagtctttta aatgatccca tatcagacta
      181 gtttgtctat ctagcttcaa tccattaaac gctaatgaac ggttcatttt ttctattact
      241 ttagataaag ctacagaaat ttttatacct tcacaacgcg catcttggat ttcaataata
      301 gtatcatcaa ttagtttttg aatttcttgc ctatcaacat attcaatctt agaaagagaa
      361 tcttttaaag ctactaatga ttcaacgatt tcttttttct cttcagacat tttcttcctc
      421 cttctagtga cccgtaaagc catgtagagc agagtccatt gaaccagtaa ttacactatc
      481 tacaacgcca ttccaattta ctctacattt tccatttttc agatttgtcg gtccatacgg
      541 atagcctttt tgacattcaa tgttcggatt ataccagata ggattatacc attcagccgc
      601 agaagcagat tcagtcgata caaacaatcc cacaccaaca aatccaatac acatcacact
      661 atagccaatt agttttttca tctcgtatcc atctcctaaa aaatagaata gttcttatag
      721 ttattcataa gacatatttt ttccgttttt gttttatcct ttctgtccta taacaacttt
      781 tttcaagtaa agaatgacat agtttttctt aaaaaaacag tctgtttttt tctatttctt
      841 cggtgtataa taaatatgag tgttcatttt ttattttaat acgcactccg tacataataa
      901 atcttgatcg catcaatctt ttgagcttct tgatctactt tcaattgaag taatacaaat
      961 tgagtaacct tattcattgt ttcttctcca aataacaaat attgatccgg attctctata
     1021 tcttgtgtca cctggtaaag tttcccagtt gttttcattt gtacatgcgg gtctacatca
     1081 atactattgt ctttaataca acgatctgtc atatagtccc tcacagcttg attgcgttta
     1141 taaacatctg tgaaattact ccattttcta gcaaaatctt ttaagaaatt ttttgtcatt
     1201 tcatctattt gcatttgttg cgctgtctcg tcttctaatg aaccaatttg actgttactt
     1261 tcagtacttt gaagagttcc acttgtttct tttactgctt cgattgtttc ttctgtcgtt
     1321 ctcaattgtg ttttttgttt ttcagaagaa aaccaatgac aaccagtaag acctatcaaa
     1381 cataaccaac taaatactaa aattatccct tttttcacgt ttctagtcac tgcctttctt
     1441 atttcacctt acgaaccaga ctacgaattt ttgtttgatt gtacgtgcgt tgataccgag
     1501 ccataatccg tccttgctcg gcattctgtt ccacactaga gaaatgttgt ccgccatatg
     1561 aaacatctgt tataattccg gtgtgaccat agattccagg agacaatgca ccacctgctt
     1621 gccaattcac cacatctcct gcttgtaaat cacttggttt tgggttcatg attacttgcc
     1681 aaccataggt tgtccagtta taatcttccc ctattaacat cgcaaacatg tgtccagaac
     1741 ccattaactg tgggccacct aattgagata cataccatgc cgtaccacca taacattccc
     1801 cattatttac ggatcgtcca atgatccgat ccaaaacctt cgcattgcct ttactttttc
     1861 ctgcaataag cgaagttttt tttgttggta ggctattcca atgcttgatc gcaaaagtat
     1921 catattccgt taagttatag gacgttatga tactcattgc tcctcgataa tacggactcc
     1981 cttcggtaat acctgctgcc gcatagcctc cacgacctag aatcaataaa caacttccag
     2041 gatctttatt gcccacacat tgattaaagt tgctttggct cgccaccata aagccataat
     2101 agtcataaca agcttttaag ttcggaaact tcatatagaa accaccttca gaaccaccaa
     2161 ttccacgtgc agaaccttgt gggtagggac aaccaggaaa ccaggtaatc ccaaaaaagt
     2221 tcgtatcgtt tcttccagat tgtgactgtc cataagctga ttcatagcct aattgagaaa
     2281 gaatcacact cggtaataac tgatacttta cagctgcacc aactgctgct tgcgcatgtt
     2341 catctgtgat tggaccacgt cctttaatct ctgaaaaaat cggtaatcct tcggtatatt
     2401 ccccattaaa agcgacagct agacctgtag cttctcctcc atgctcagaa agttctcctg
     2461 aaaaaaagaa tggcaataga aaaaagggaa gaataaagaa aagaagacaa cctagacacc
     2521 ctttcttcat ttcttcctca tcctttcttc gataatggtt tttggactgt tcgtcctttt
     2581 ggtaaagtaa aatcagttcc tggttttgcg tccaaaatcg gcagtgtttt aggatccttt
     2641 tctatttttt ccgattgctc tgttggctta tctaaagaag aaaatgcagg ttgtaacgat
     2701 tttggtgcgg aaatcgacgg tttactttca gacgaacgtt gccccggttg ttgcacagaa
     2761 cgatttcctg atagagtggc taccgacgaa ttaggaacag agtattggtt caataactgc
     2821 tgcattgctg gataactccc atttttcgcc tgatttttca cttggcgaat ctgttgtgaa
     2881 agagaaccag cagctgttaa ttgtggcacg ggtacttttc cagaaggcaa cggaatcgat
     2941 tgaccaacaa aagcgatacc tttatttaac aatggttttc tttgattatt cccttgttgc
     3001 ttactcaatt gtgccaatga ttgtacctgt tgttttggtc ttactgaatt catggacgaa
     3061 tttggaatca aaccatttga aagaccagaa ttccactctg ttttcgattt ggtagaagac
     3121 atttcaggct ctaccccatg ataacgaaca tgacgaccac tataaggcaa ggcttgattt
     3181 gaacgcccac ctagttcaaa gggattaacc acttgcctcc cttttaccga ttcttgtggt
     3241 gtacgttttt gttgaaactt ttgaaatcca tgtatcgctg cagaagcgag tggattcgtt
     3301 gctcgataca cattccctgc taatcctgca cctttgattg ccaaattttg tccagcagtt
     3361 gatagttgac caggcagttg tttcatttga gacaaagaat gacttgctat gtttttcgtg
     3421 ttgtttatag tttgatctgc agctcctaac cctagtttct ttaataaatc tttccgtttt
     3481 acaatgagta aaattgatag gagtgctttg acaaacatat ttcctaaaaa accaaaaaca
     3541 gtattcattg gaattaaatc gtctactatg ttatagatca aaaatgccac caaaataatc
     3601 acactgtaaa aagatttttg aaacatcaag cccaaaaagg actttaaggt attcatgagc
     3661 atataatcaa atactggaac aaaagaagcg agtaatgaaa aaggaatcac aagtaataaa
     3721 cacaacacca acatttgaaa gataaaacga atcatgccaa tcactacaag tggtaatcca
     3781 atacttagat taagcaatgg caccatagaa ccaacgactg ctttataaac agctttagtt
     3841 ggttgaatat agacacgata agccttcttt ttccctctat cttctcctgc agcagtggtc
     3901 acttttgtct caatatcaga taattcttca cttgacgctt ccttggctag aaattcttgt
     3961 ggttcaatct ctgcttgata caattcttct aaatttgttg tcccataatt taacaatata
     4021 tacggatcaa caacagccgc tttataataa agttggcgca ctttttgaat ggcttccttt
     4081 tgtgtcgttg gcactaaaac accattgaca tcttttgtcg ccaattcatc tagactattt
     4141 gtagcagaaa aaataaagcc ttctacctcc acggacaaat catttaaccc tttggtaaaa
     4201 gcttctcctt tactaggtac gttactacca tagccaaacc aaaccattga aaaaatgaca
     4261 acgaagagaa atcgaataaa caccattttt gcgtaatgtt tccctctaaa tatacgaaga
     4321 gtaaatagat ataacacata caagacgaca atccccgcac caataggatc gtatattttt
     4381 gaataaatag tatccgaaaa agtgaaaaac tggtgaatta actcgttcat tgcagctcct
     4441 tcatagagct tctcaatgac aaaatcaagg ccttgccaaa tcaatttact taaactaaag
     4501 aacatattcg tcatcatttt tagaaaatct gtgccatttg ccccaaataa cattttgcct
     4561 aatgaaaatt cactatcaga taaataagaa gaaaaggtat caatatgaaa tttcccaatc
     4621 tcatagatcg attgatccac ctgcttttct gccgcataca ccaatccacc aacaaaaagc
     4681 agtccgaatc caatcgttaa aaaaaacaaa acgcaccatt tcttttcctt atgctttata
     4741 taagccattg tttcacctca tttctattgc atgattaacg tatcctgttc ttctacttga
     4801 gagtgacttg ttttttccgt tgtttgtaat gcagcggtca tttcttcaaa caaactgtgt
     4861 acattgagtt ttcctacacg tccataggga tctaacatcc aacactgtcc tttaatcatg
     4921 tggctcattt cagctaagtt cgcttcattt acttccaaac gtaaatgatt caaaatcttt
     4981 tctcgctcac tgtcttcatc aaaagcaaag acgacaccaa aattcccttt ttctttatct
     5041 gaacctaagt caccaataaa ttgtgtatct aacactaatt gattcatctg ggaacgcccc
     5101 acacgtgcca ttgctgcaat aatactacgt cctgcgttcg aattattaaa tacccatgct
     5161 tctgtaaaat aaatcgctgt tttcttacta ctatctcgtt ttccaaacat ttcacaaaaa
     5221 cgacctaaac taatcatcaa acacaaagac tttcgatccg catctgtata tagttctggt
     5281 cggacattgt catttggtaa tttcaaatct tggatctcta aaatcgttgt tttggcatta
     5341 aagtctaatc ccgggttttc tccataagaa aatcctaaac gtaaaatcga atcttctacc
     5401 gtcaagcgta aaaactcacc atattctcga acctcttttt gttccatttg ttctaaatag
     5461 tcgaccacat gcatcaagcc aacttgttgt ccggcttctt tttgaactaa aacttcttgg
     5521 attcccttta ataccgcccc ttttaaaagg tgattggtac ctaacggact taactcatca
     5581 atcatatcct gggctacttg tttcgcatct actcgggata aacaaacaat tggatctaat
     5641 acaccataat tacggggatt tgtagcatct aacgtgacat aactcatttt ttgcaaatgt
     5701 tctacaaaca aagggtaatg tttctgataa tacgggtctt gaatgagttt cgtaaaccat
     5761 ttgcgctttt ctcctttggg atccacaaat aaactttgta cattcataaa cgaactatac
     5821 aaaaagagca agccaactaa aaaggtcttc cctttaccgg tttttccagt tactgctaca
     5881 tgaggcgcat caaacaacgc acctttgatt ccttccgcaa tggctagaag attcaaaaag
     5941 acaaaacgat ttgacgcata aattaattct tctaatggtg ttttttctcc tcgagaacca
     6001 ccatccccta atgtatcttg ccgaccaata taccagccgg attccatacc taattctgat
     6061 actgctgaaa acaaccattc gccaactgct tctacagttg ttcggtgctt ccaatgtaac
     6121 tgccgtccca atggttgccc taataaacat ttatagaaca attctcgttg atcatttttt
     6181 gcacggtgga tatggataat cgtatgaagt tgttgaataa ccgttgcact attttgacga
     6241 acttcttcaa ccgtttgacc gtatacaata aaacatgcga tccaatcgac gacaggttgc
     6301 ccattatcta ctcgttgctt taaatgtttt actaatcctc gaacagttaa taagcgttca
     6361 ctaggttctt catcattttg aaccaattcc ttatgctctg ttttgaaccg ttccgatagc
     6421 caattcagtt ttaaaccaat acctccctgt cctttcaagc gtggatagtg aattttcatt
     6481 cgcaattctg ctgggaaaga aagatcctga gcaaattgaa atagatcaca atttctcata
     6541 ttttcaggtg tttccccaat aggcaacaat gctaaataac tactccctaa ctcatcttct
     6601 aattgaagaa ggcctttatg ttccttcggt atacgtacat acgttccttg attgtctttc
     6661 agtacttccc aattattttg ttcttctact ttgtgagcca ttcctcgaat atattgcaga
     6721 cgattgagat acaacagctc tttttttact aagggacgaa tagaaaagct ataaagtaat
     6781 tgtgtcactt cttttacata aggatcactt tcagaaaaca aagtttccaa atcatctact
     6841 tctaatccaa ataagcgtgc taaacttcct gctaaatcta accagattag atttattaca
     6901 tcatgaaaac tctccaccgt tgctttttct ttgactttga ttcctaaaat aaatgaagga
     6961 cgatagactt gaccttctcg ttccaacaat gtcactgttt cttttgcata atacgcacca
     7021 attgcttgac tatcgggaga aaatcccttt cctaatgtgt caaacttcgt ccgtaaattc
     7081 attgttcgtg gatacatcac caactccaca tcttcataat ctgctaaaag caacagtaaa
     7141 cgagccatca tttcttttat ttcctgtgtc ttttttggtg tgttttttga aattgttcgt
     7201 ggaaaaacat gatagtaggc ccagatacaa tcctctccat ttaacaaaca atttttcttg
     7261 aatgctttta ctggttcttt tacaagcacc aatgaatttc acctcccaat aaaaaaggaa
     7321 caaccagttg attgtccctt tttctaatcc tctaaataaa catacttatt cacaaaaagt
     7381 aattcttcca atgcattgtt tgataccttg tcccagttat aatacacaca atcaccattc
     7441 atcatacgaa tcatataaca aaaccaatcc cataaataaa aatgcacttt ttttccatca
     7501 ggttttagtt ttgccagtgc taacacacat aaatagggga gtcctatata aaaaacaact
     7561 cctgctggtg gaaaaaaaga aatgacccaa ccaataaatg aacgtaaaaa taaatattca
     7621 aagattacga ccaacaaaaa agttgctgca ttgactaatg caactggaaa tggaagacgc
     7681 caattgcttg taatctgttg tacgcgataa ggttccgcat aaacaatcga ataatcataa
     7741 ggttcccaat ccacttcact atcctcctaa tccataaata atttttcaat gattttacca
     7801 aacgcatcaa atacacgtgt tggatcattt aagaagaaat aaactgcggc acctgctgcg
     7861 atagatatta atgcatttcc tacttttgat ttcataaaat ttcgcaatcc cagataaaca
     7921 accaccatta ttaaaaaata gccaccttgc gttaatataa aatctacaat tcccttcagt
     7981 gtcatttccg ttcttctcct ttcctatcca accaattatg tgttaatttt tcaacataat
     8041 actgatttgt ttttttcgta atgacaatcg tcatttgttc tttttgtaaa agtgacgtat
     8101 caccatctcg aaagacgacc gttgctttta caataaaagg ttttttcgtt tctttataac
     8161 gcaatacttt catctctact tgatcaactt tagctgtttg attgagtcct tcaggttctt
     8221 tcatcatgta tgacatatct tttgcattgt ttttcgcata cttatctaag aaatcttcca
     8281 caaatccttc aacatctttc gcttccgacg catcaaccgt ttcgtataat gtgtcatcat
     8341 aagtaatcat tcgttgtttt tctgtattct ttggtagtgc cgtaaaataa ggataagcaa
     8401 taatacgaca gcccttgttt gaaatagaaa agggaagatt gagtacttgt gtgattgtca
     8461 cacttgtttc tttcttactc tctggattca tcgagcgaat atcataagtt actttataac
     8521 tagcagttgg aatggtttca atttctttta atccaataaa cgaagcagca gttaattttc
     8581 gaacacctga aacacttcct ggttcttgaa taaagttttg tgcataaaac tcttttttta
     8641 atgtttcttg acgttgttgt tc
//
LOCUS       DAHHZJ010000120         8588 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.121, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000120 DAHHZJ010000000
VERSION     DAHHZJ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.121"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            474..725
                     /locus_tag="NBL02_002140"
     CDS             474..725
                     /locus_tag="NBL02_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572817.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
     gene            759..1037
                     /locus_tag="NBL02_002141"
     CDS             759..1037
                     /locus_tag="NBL02_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572818.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            1634..1891
                     /locus_tag="NBL02_002142"
     CDS             1634..1891
                     /locus_tag="NBL02_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572819.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            2004..2615
                     /locus_tag="NBL02_002143"
     CDS             2004..2615
                     /locus_tag="NBL02_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572820.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            2794..3351
                     /locus_tag="NBL02_002144"
     CDS             2794..3351
                     /locus_tag="NBL02_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572821.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(4050..4571)
                     /locus_tag="NBL02_002145"
     CDS             complement(4050..4571)
                     /locus_tag="NBL02_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HCD2572822.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            4770..8462
                     /gene="nifJ"
                     /locus_tag="NBL02_002146"
     CDS             4770..8462
                     /gene="nifJ"
                     /locus_tag="NBL02_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCD2572823.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
BASE COUNT         2898 a         1386 c         1822 g         2482 t
ORIGIN      
        1 acaaataatt ggtttattct ggcaggcgta atgctttttt ctagtttcta tttatttagt
       61 aatgctctat tagcacagcg agggaaatat aagtttaaaa tattagatta gatattattt
      121 ttagagggga taaatataaa atgaaaagag gttatttata atggaaaaag tattaccaat
      181 taagagctac tgtattccaa ggcttttcat catagaattt caaaagtaaa gacatgtgtt
      241 tcacaaatag tgtctatcct ggctaaataa ttcttccact atctgcttta atttcagtag
      301 acttttcggc aagttatttg ctaatgagat gaatagagat tacaactcgc ctataaccgc
      361 ttaaaacaag cttttttaaa gtatagttag caagtcgtat tcatatgttc aaagaggagg
      421 gcttatgaac tttgaattca ttccgtgtga acagaacagt agggggaagt aaaatgaaag
      481 aaaagcctat aaataaatgg tcattgtata aaaattcaat gtttggagtt gttatctttg
      541 gtgtttgtgc tggattattc cttttcatat tattacaaac ggtgactctt ttctcttcta
      601 atcctactta tggatggaga gacagtatag ccttagggat gtttgcggga ggatatggat
      661 tcttattgtt gttctcaatt ctcacactaa gtccaatcca tggactcatt caactttgga
      721 agtaagaacg agccttaaac ttttcgttct cggaagaaat ggaaaaacac aacataacag
      781 gcagtatgta tgtggatgat caatggttta tcagcactga tgaggtaaaa atagctgcca
      841 ttaggagaga ttacctgcag aaaattcata aaataaaaga tcctaatggg aaaaaggcaa
      901 ggttaattat aactttgata acatctaatg gtaagaagtt aaaacttgca ggatcatatg
      961 ataatgttaa aaagttcgaa cattggtatc tgcaaaaaga cagcattagt cttaataaac
     1021 aatttgaaaa aaattaatca aagtcatcgg caacacgaat aatttgacat cttgatattg
     1081 tgatataagc aaaaacggca tttctgagac cgaacagcat aaaaaattta aatgttggaa
     1141 tttatataag caaaacccaa gaagctagat gcgatagagc atttaatttc ttggggtttt
     1201 gcttgggaat cagcatatca attaaaatga tcatctcggt atttcaaaag tgggtacgat
     1261 tacaaatagt tggagcaaaa tagtgggtaa aattaccatt ccagtgtctg tcttgcctct
     1321 ttttcgtcaa tcacaaatag acgagattca taggaaaaat cgatacaggg tatgatttca
     1381 atgatattat gaccaattac aaaatagatt gataaagcat tttttgcatt tttataatag
     1441 tttattgata ataatgtatg agatacagta taatgattta aaaggtaacg tgttgtgacc
     1501 gaattaaatg aattaatgcc tattttttat attccttagt ggacaaaaat ggagacagtg
     1561 aagttaggag cattgtatcc tttgttgcgt agttcaaaaa agtaagcgat ttctataatg
     1621 gtcagggtaa acggtgaggg ctgtccacgt aaatcctact attgaaatca caatgatcgg
     1681 acattcaaaa ggtggtgctg aagcagcaaa ttcagtagct ataaatacga atgcgattat
     1741 atttaatcca gcgacagtaa atttatctgc ttatgaatta gactcaaaca catatacagc
     1801 aaacatggta gcttatgttg tcgagggaga tgcattaaat gttgtttttg gaggagtata
     1861 cacctattgg agaattaatc atgttagata gacaatatga tgcaccttag tggagaatga
     1921 tatctcgcat aggtaactca gtaaaaaatc aaggtatgga agcaattatc tctgcattgg
     1981 aagatcgata tagataggcg gatatgaata gtaatagggg gaataaaatg gaatacaatg
     2041 gatcaatgag aattgtacaa attatatttt ggattacatc gggaattgtg atcataggag
     2101 gagttttttt gatgttgcca agcttaatat ttcctttctt tgcattaatc tcacctaaaa
     2161 tacccgaacc ggaaatcacc tatggagaat ttccatttag gattgaatac gaactagatg
     2221 gacagattaa cataattgaa gatactgtca tagctgagtt taacgggtgt gagttcagcg
     2281 caggttctat gaaaagagaa cgaagatgga gaagtagatt agctagtggt agagaagatc
     2341 taccttttgg ggacgatcta ggaatctatt ttagtcgagg aagtgcgcaa tattatatgg
     2401 gggaaaatgt acaatcaatg tcattaaaac cacatatagc ccttagaaat ctcgaggaag
     2461 gttttaaaag agaggtagac tttatattag gagaaagtgg atacataaga actgtactta
     2521 atttcggaga agcgcaagaa gttttgacac aatacggaat taccttaatc aactgggaaa
     2581 tatctgaacc gattgttaac aattttggtg attagaaaac aattacgata aatagaaaca
     2641 agccaaatca tcagcaagta aaagctgatg atttggcctt tcttatacga gtagtaggaa
     2701 ttcatagcga atattattac gaccagtaga tattttgata ttaaaggagt attacgaact
     2761 aatatgtgag aatgtagagg tgatgtgcga ataatgagaa atttatcaaa tagaaacaaa
     2821 atattaatca ttattgttgt tatagctgtg tttcatctgg gaacgaatgc ggttttaagt
     2881 agaataatat tgggtcctaa gccaccaaga ccagaaatta cccgagggga gtttgatttt
     2941 agattagaat atgaagtaga tggggaaagg attgtgatag aagatacaat tgttgcatta
     3001 tttgatggat tttctgcaga tgcaggtagc atggcatggt atcgcacgtg gcgtctacat
     3061 ttagcaagtg atagacgatc acgaaatatc ttattagatg aattagagga tggtcgaaga
     3121 atatattatg tcccagaaag tgctaactat tctatgggag atgtacagaa aaaaagagag
     3181 cctaatccta attggtatcc atttaatggc gtaacaatag aatatccacg caataaaaca
     3241 cctgaaatag gtgcaaaatt tataagtggt ttagaagatc tgtacgatag gtttggaatc
     3301 cgtcttatta gttgggaaca cgatccacca atagaaaatc gctttgaata atcgaaatct
     3361 actaatccat ttcatcagcc agtgaatgtt gtaatggaac aatttggttt agtgttcaaa
     3421 accattgaaa tactaggctg tgatcagcaa aaatgaatct gaataaaaaa gagagcaact
     3481 attagctgag aggagttaat agttgttagc tttatacgaa tcaaagtggc aaatacagat
     3541 gcgtatgcat ttgtgtttgc catcttgcgt ttggtagacg agataggtat aagctaaaga
     3601 aacaacttgt ttaaaataaa cgacctagct caacaaatga ccatctaatc aactaacagc
     3661 atacataaaa gcaactattt ttgaagtaga attgaaatcg ggataactta aaaagtgatt
     3721 gggatactag tgagaatcac cgtacataaa tattactcgt taagggaaat tgagaaattt
     3781 tgtatgaaaa tttaagaatt agttagtatg cgcaagtcaa aaatatgaat aaattatttg
     3841 gtggagataa tttgcttagc ttgtcataag aaatgagata atctggcaga tgcaagcttt
     3901 tggacaccat ttattcggaa taaggaggct gggacagaag cgtttaactc caagaaataa
     3961 gaaggagttg cttctgcttc cgccgtttat acgtgtttag agcgtgagac aaaagtgttc
     4021 tttacttttg tcccacgctc cttatttttt tattttgttt ggatcaattc ttttaattcc
     4081 tctattgaaa tagattcatc aaagatttct ttacccaaga tgacagttgg aacaaatcga
     4141 atatgtgctt gctcagcttc gttgatgaca gcttggctca gctgttcgtc tttttgttct
     4201 gtcagcttta gtttatctac tgcaaaatcc gctacttctt ggagagaaag atgtttccac
     4261 tcgtcttgag tatcgaatat ttgtttgatc tctttgatcg ctttttggcc atcgctgatt
     4321 gtgagatacc ggtgcatcac gttccctcgc tgtaagcttt ctttttcttt atcgaacaat
     4381 ttgatcactc gctgcaattg cccgctttgg actgcttctt ctaatagctc ataagattct
     4441 tcaaaccatt gtcggcaata cggacaagct aagttgatga attcgaccat ttttttagga
     4501 gcattcgatg aaccataaag gattcctttt tgtgtatttg ttttcgttgc atcaatcact
     4561 gatatatcca tgttaaaaca cctctttcgc ttatagttta tacatgtctg acaaaaaaag
     4621 gcaacgattt agactgtttg tctaaaaatt agatgaccaa ttcaaataga tacatgtaaa
     4681 atatttttat aaataaaaga aaaaaatggc atgttttacg ttataatgga acagataata
     4741 agagagaata tgaacaagga tgatgaatca tgcgaaaaat gaaaacaatg gatggcaata
     4801 cggcagccgc atatatttct tatgcattta ccgagattgc tgcaatatat ccaataacac
     4861 ccagttcgac catggctgaa ttagtagatc aatggtcagc agcaggaaaa gaaaacgtat
     4921 tcggtcaacc agtaaaagta gtagaaatgc aatccgaagc aggagcagct ggtgtggtcc
     4981 atggatcatt gaaaactggt gcactgacaa ctacatatac cgcttcacaa gggctgcttt
     5041 tgatgatacc gaatatgtac aagattgctg gggaactact gccaagtgtt ttccacgttg
     5101 ctagtcgggc agtgacgaca aatgcgttga atattttcgg agatcacgga gacgtgatgg
     5161 ctgcaagaca aacaggattt gctatgttag ctgaaagcag tgtacaagaa gtgatggatt
     5221 tagcacctgt tgcccatctt gcttctattg aaggagaagt cccttttttg aatttctttg
     5281 atggattccg tacgagtcac gagattcaaa aaatcgaagt tttggaatat gaagaattag
     5341 ctcctctgat caatcaggag aaattagctt catttcgtaa acgagggatg aatcccaatc
     5401 atccgactgt tagtggtacg aaccagaatc cagacatcca ttttcaacaa cgggaaacga
     5461 tcaaccccta ttatgataaa ttacctgaaa tcgttcaaaa atatatgaaa gagatcaacc
     5521 gattacgagg aacttcttat gacttggtga cttattacgg agcagacgat gcggaagaag
     5581 tcattgtttc tatgggatca gttgcacaga cgattgaaca aacgattgat tacttgaatg
     5641 caaacggacg gaaaacaggt tttttgaata tccatcttta tcgtcctttt ccagtggaaa
     5701 cattcgtgca aaagttgcct catacagtaa aaacaattgc tgttcttgat cgaagtaaag
     5761 aaccaggggc aggtggagaa cctctcttat tagacgttca aagtgcgtta tatgattctg
     5821 aacttcgccc ggctgttatt ggtggtcgct acggattggg gtctaaagat gtgactcccg
     5881 accaaatctc agcagttttt gatgaattga aaaaagaccc aagcatacgc aaaaaacggt
     5941 ttaccatagg gatcgtggat gatgtgactt atcaatcttt ggaaccaaag gaaagtctgg
     6001 atttgacaga accgcaaaca ttccaggcca agttttgggg atttggatct gacggaacag
     6061 ttggagcaaa taaatcagct atcaagatta ttggagatca caccgataaa tatgcgcaag
     6121 gctattttta ttatgattcg aaaaaatctg gcggtctgac tgtctctcat cttcgttttg
     6181 gagatacgcc gatacgttca gcgtatcttg tcgagcatgc ggaccttgtt gcctgtcaca
     6241 caccagctta cctgcatagc tatgacttgg tcaaaggatt gaagccaggt ggcatttttt
     6301 tattgaatac tctttggagc gacgaacaat tagaaacgca tctgccctta aaattaaaac
     6361 gttacttggc tgaaaataac attagattct atacgatcaa tgccatgaga ttggcccaag
     6421 aagtcggttt gggacgccgg atcaatacgg caatggaaac ggcttttttt aaattagctg
     6481 acatcattcc ttttgatgag gtgcttccgc tgctaaaaga agaagccctg aaaagctatg
     6541 gacataaatc aatggctatc gtagaaaaaa acatacaagc aatcgatcgg acgaccgaac
     6601 tgcttcatca agtcgcgatc ccagaccatt ggagaacgtt ggagatgcct gaaaaaatca
     6661 ataaaaggaa cactggttat atacaagaaa tacttgaacc agtgaatgca caggaaggaa
     6721 atcaactctc tgttggtaca cttgtagcaa atggaatgac aactggcgaa atgcctttag
     6781 gaacggcttc tatggaaaag cggggcgtcg ctttagaagt acctgagtgg attagtgatc
     6841 gctgtacgat gtgtaatgaa tgtgcctttg tttgtcctca tgctgccatc cgtccatttt
     6901 tagcagatga agaggaaatg gaagaagcgc cagaaggatt tatcgtgaga gaaatgcgag
     6961 gcgcagacgg cgtaaaatac cgtatccaag tttcagtgga agactgtaca ggttgtggtc
     7021 tgtgtgtaga ggcttgtccc gcaaaagaaa aagctttagt gatgaagcct tatgaagaac
     7081 agaaagaaca agctgtgaat tgggcttttg caatgacatt aagacaaaaa gcaaatccag
     7141 tcaaaggaaa gaatactgtc ttgtctacac aattcaatca accgcttctt gaattctcgg
     7201 gcgcttgctc aggctgtggc gaaacacctt atgtaaaatt attgacacaa atgttcggtg
     7261 atcgaatgat gatcgcgaat gcgacaggct gttcttctat ttggggcggt gcttctcctg
     7321 cttctcctta tacgaccaat gaatgtggtc aagggcctgc atggtcaaat tctttattgg
     7381 aagataatgc tgaatttggt tatggtatgc ttctagcggc acagactcgt cgcgaggctt
     7441 tggcaagttc aatcaaagaa gtattgccaa aagtatcaga agaactacga gaattgatgg
     7501 aagactggtt agtacacatg tttgagagtg aaggaacgca gcaaagagca gcaaaactgc
     7561 aagctgcgat cgaaagcgaa cgatcaattt tccctgaact ggaggatata tacaagagaa
     7621 aggatctatt cgtaaaaaac agccaatgga tgattggtgg agacggatgg gcttacgaca
     7681 ttggttttgg cgggatcgat catgtacttg ccagcggagc agacgtgaat atccttgttt
     7741 tggataacga agtctattcc aatactggcg gtcaaacctc aaaagcaaca ccagcttcgg
     7801 caatcgctaa attttcggct agtggaaagt atgcttcaaa gaaagatcta ggaatgatgg
     7861 cgatgacata tggaaatgtt tatgtggcgc aaattgcttc aggtgccaat caaatgcaaa
     7921 cgatcaaggc atttgaagaa gcagaaaagt atcctggtcc ttctctgatt attgcctata
     7981 cgccttgtat cacacatggc ttgcttggcg gaatgaaaga atcgcttaaa gaagcaaaag
     8041 aagctgtttc ttctggttat tggtcattat atcgatataa ccctacaatg gtcgaaaaaa
     8101 acaagaaccc aatgactttg gactacaaaa aacctgattt ctctgctatg cctgatttca
     8161 tgaggaaaca agttcgcttt tcctcgctag ataacgcaaa tccaaatctt gcaaaagctt
     8221 tatttgaaaa gactgtccac gacgctaaaa cacgttttta caactatgct gcactgacgg
     8281 gagaattaga aaaaatcaag aaacgacttg aaccagagga atctatcgat gtacccgtta
     8341 agactagaaa agaaaaaacg acggaccttg aagcagaaga aagaagagct gctagaagag
     8401 ctgccagagc agaacgacgt aaaaaaatgg aacaagctac aaaagaaaaa aaggaaaaat
     8461 aacagaaaaa atataatgaa taattctctg aagtttaaaa atatttattg cagagaaaaa
     8521 taaaacgcat acttgcaatt ttcttgaaaa ttatgcgttt ttctcaagaa aaatacgagc
     8581 atatcagc
//
LOCUS       DAHHZJ010000121         8528 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.122, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000121 DAHHZJ010000000
VERSION     DAHHZJ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.122"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(<1..768)
                     /locus_tag="NBL02_002147"
     CDS             complement(<1..768)
                     /locus_tag="NBL02_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCD2572824.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQ"
     gene            914..1717
                     /locus_tag="NBL02_002148"
     CDS             914..1717
                     /locus_tag="NBL02_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HCD2572825.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            1895..2842
                     /locus_tag="NBL02_002149"
     CDS             1895..2842
                     /locus_tag="NBL02_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCD2572826.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(2910..3203)
                     /locus_tag="NBL02_002150"
     CDS             complement(2910..3203)
                     /locus_tag="NBL02_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HCD2572827.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(3231..3896)
                     /locus_tag="NBL02_002151"
     CDS             complement(3231..3896)
                     /locus_tag="NBL02_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HCD2572828.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(4036..4668)
                     /locus_tag="NBL02_002152"
     CDS             complement(4036..4668)
                     /locus_tag="NBL02_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD2572829.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(4675..5742)
                     /locus_tag="NBL02_002153"
     CDS             complement(4675..5742)
                     /locus_tag="NBL02_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCD2572830.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(5739..6470)
                     /locus_tag="NBL02_002154"
     CDS             complement(5739..6470)
                     /locus_tag="NBL02_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HCD2572831.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(6639..7118)
                     /gene="greA"
                     /locus_tag="NBL02_002155"
     CDS             complement(6639..7118)
                     /gene="greA"
                     /locus_tag="NBL02_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCD2572832.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(7254..8429)
                     /gene="mltG"
                     /locus_tag="NBL02_002156"
     CDS             complement(7254..8429)
                     /gene="mltG"
                     /locus_tag="NBL02_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HCD2572833.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
BASE COUNT         2531 a         1757 c         1325 g         2915 t
ORIGIN      
        1 ctgatcttca aaatcaatat gtccgtcgaa aataattatc ttttcgctat actgccaaat
       61 atggacatga tgaacatcct ttaccccatc gattgcttca atttcttgtt ccattttttc
      121 atagtcgata gcaggtgcac tttgtatcaa aatagcggca gcttttttga tcaccattat
      181 cgcttctatc aatatatata tcgaaatgat cagtgtaata actggatcga tccagtagat
      241 atcgaaaaaa cggatcaaaa ttgctccgat aatcacactt accgaagaca gtgtatcact
      301 taacaaatgc aaataactag attttatatt caaactttca tctgcttctt gtcgtaataa
      361 taatactgag aacaaattcg cgagcaatcc aataactgct actgttagca tcaaatgact
      421 atttatcttt tctggtgaag tgaaccgctt gaatgcttca acggttaaaa acaaggataa
      481 tatgatcaat atgaatgagt tgatgaaagc actcaatatt tctaaacgct ggtagccata
      541 cgtacgtttt ttatttgctt cttttcgggc cattttttgt gccccgtaag aaaaaataat
      601 tgccaatgta tcactaaggt tatggaaagc atctgaaaca agagacaaac tccctgacat
      661 aatccctcca ataaattctg ataaagtgat caccgcattc aaaaccacta ctccaaatag
      721 tttttttgat gatctctgtg gctgtgtagg cactattcct tcctccattt tatttatatt
      781 ggatagtctt tagacaagaa taacattttt ttgatatttc accaatcatt cagctgtttc
      841 cttaaagcac ttgtatttca cttgatactt cgctagaatt gcaagtgatg acactactag
      901 atgaggagta cttatgacaa caaaacaatt aattatcagt tcctttatac gattcaaaga
      961 cctaaaggag gcgagaaatg tcccattttg gaaagccatt gtctatcttt tagttttgag
     1021 tataattatg gctttgccaa tctcttttca agtttttcaa gtcttaaaca acattcaatt
     1081 ggatgggcaa aagattgcta ctcgcattcc tgattttaca atccaaaacg ggcagcttca
     1141 aaccgaagaa aaagaaggat tcatttacca aacaaactcc attatcttca catttgatcc
     1201 agagggaaaa cgaacagaac aggacatctc tactgatttg atggggaatt ttgttagtgt
     1261 cgggatgtta aaaaacaaac ttatcatagc tcttcctaat acaggcacga cttctgcttt
     1321 gttgaacaat aaccaattag aacttcccta caccaatgaa tctttaaaaa acttaacagg
     1381 taagcagctc cgttcttttt taagtgaagc atctatacca ctttggataa aagtcctgac
     1441 atttatattt tctatttatc ctagttttct gaatctgctg atcactttgc tatttaccaa
     1501 cattgctgct tttctctatg cacgctttcg actaacaaaa gctacttttc ttgattgttt
     1561 aaaaacattg atctattcaa tttcattgcc tactataatt gcaacaatct tgatggtctt
     1621 tcttccttct tttgacacta gcgccttcat tgcttttgca ggaatcttta tttttgctca
     1681 agctgtcaaa ggatgggcaa aaatatcgat caattaagaa ataaagacaa ctcgttattt
     1741 ctacttcaat agaccctttt agaggttgtg acattttttt gtcacagccc cttttttatt
     1801 tgaaaaatat ttctttctca aaaaacaagc aaggcgcttc aaagttcttc ttgtatgcgc
     1861 tacacttaga gtaaagaaag gcgggaaatt aactatgtca gaaagaatca tttatgcaga
     1921 tcgtccattt cgtgatgaat ttatcgaaga aatccatact atagcgcctg gttatatctt
     1981 taaaacagaa ttacaaccag aggatctaca aaatgtagag atcagtttgg gctggaactc
     2041 agactaccaa gaaaaactat tagcctcttc taccttgaaa tgggtccaat ctatctcagc
     2101 tggtgttgat cagctacccc ttgagacttt ttcaaagaaa catattttac tttcaaatgg
     2161 tagtggcatc cacagtgaat cgattacgga gcatgtctta ggaatcatcc ttgggtattc
     2221 tcgtggtctt tttcaagcac aaaaggcaca gagtgacatg aaatggttag gttcatcggt
     2281 ccattaccaa tctatccaag ggaaaaaaat actaatcatt ggaactggcc atattggaaa
     2341 aatgattgca aaaaaatgca aagcattaga catgaactgc tatggaatca atacatccgg
     2401 tcatccagca gaaggaatgg aaaagacttt ttctttaaat caactaaagg aagttcttcc
     2461 tgaagtagat ttcgtagtca atatcttacc tctaacggag gaaacaactg ggctctttga
     2521 tcagcatatg tttgaacact ttgatccaaa atctgtattc atcaatgttg gccgtggtgc
     2581 ttctgttaag acacaagatc tggtgcaggc tttaaataac cagcaacttt cctttgcagc
     2641 attagacgtt tttgaggaag agcctcttcc acaagatcat cctctttgga aaatggacaa
     2701 tgtactgatt acttcccaca ttgcaggttt gacacctgat tttcaaaaga aattaatggc
     2761 tattttttta accaatctga aaagctactt tgccacccat gagcttcaaa ccaatcaagt
     2821 aaaattgtct gcaggatatt aatagctgaa aacaatattt tcaaaagaaa aaagagaagg
     2881 tgacaaaaat tgtgtcaccc tctctttttt tattgttttt taaagtgata ggttggctcc
     2941 tccaggttgg gatcataatc aacaagtaaa tcatatcctt taaagaacca ttcatctgct
     3001 tcctcgataa aaaaaagtgt cccatcaatt tccactttct ttacaggatg ttctggctgt
     3061 tcaactgaca ttccaatcga aaaaccatca tgaacctctg ttttgccgta gacttttcca
     3121 aaaaaacgta ttccgtaagt ttctggcaga tcaatttctg agtcaaacca cttttgtgct
     3181 tttggcgtta ccgttaattt catcccaaca acctactttc tcttttcttt ctattgattc
     3241 atcttatcgt ctagaatatc cacatcgcct gtctctccaa taaccagtag attatcattt
     3301 tcacgaacat attcatctgc tgcaggagaa gcgataaccg tttgatctac tcttcgtatg
     3361 gcgactactg ttaagtcaaa acgttgcctg aaatttaatt cagctaatgt tttgttgaaa
     3421 aatttaggat tggagactcg gatttctgct aaagaatatt tatctgacaa ctctaggtaa
     3481 tctaaaatat tctttgacac taaattatgc gcaatcctta ttcccatgtc tcgctcggga
     3541 tggacgacgc gatcagctcc gatcttttca agtactctgg catgatactc attttgggct
     3601 tttgctaaaa tatttggtac gcccatttcc tttaccatca gagtaactaa gatactggct
     3661 tgaatatctt ctccaatagc gacaattaca tgatcgaaat ttcttatgcc taatgatcga
     3721 agtgtcattt catcctgtgc atttgccaca acagcatgag tggctatatt catatattca
     3781 ttgacccgat cttcattact atcaatagcc agtacttctt gaccagcttc gatcagtgtt
     3841 cggcagatac ttccgccaaa tcgtccaaga cctacaattg caaaattttg tttcaccgtt
     3901 ttcctccttt agtatcttct tttctcagta taacaaagcc tttacaaaaa caaaagcaag
     3961 catcattatt gtaaaaaagc caagaaaaat aatagttatt ttctcagctt ttgattatcc
     4021 tctttatcat cgtttttatt tcaccaaacc atgtttgaat gcatagatag cggcttgtgt
     4081 acggtcttcc acttctagct tagccaatat attggaaaca tgtgttttta ctgttttcag
     4141 tgtaataaac aattcgtccg ctatttcctg attacttttt ccttcagaaa tcagcattaa
     4201 aatttcattt tcccgattcg ttagttcttc atgtaataca ggctcatttc gccgactcat
     4261 tttttccatc atttttgttg tcacttctgg ttctaatact cgctcgccac gttgcgttgc
     4321 ccttatagca tcagcaattt catgtgcggt agaggtcttc aacaaatacc ctgctgcacc
     4381 tgcttcaatg gcaggataaa ctttttcgtc atcaataaaa ctggttacga taatgatttt
     4441 agcttctggc caatctttta agattgcttt tgttgattcg ataccatcca tttcttccat
     4501 gactaagtcc attaaaatga cgtccggtcg aagtgtcatc gccttctcat agccttgtcg
     4561 tccattttca gcttctccaa ttacttcaat gtcttcttga atagataaat aagaagagac
     4621 gcctaagcgc accatttcat ggtcatctac taataaaact tttatcatcc ttatctatgc
     4681 ctccttcatc aaagggacct tgatttctac acttgtacct tgacctttaa aactaatgat
     4741 ttttactgtt ccaccaatac cagctactcg ttctttgata ttgttcaatc cataacttcc
     4801 cgttttcgtt tcattcatat taaaccctgt tccgtcgtca ataatacgta acaaaagatt
     4861 attgtctatt ttgtgcaaat atacctctaa ttcattggct ttagcatgtc ttaatgtatt
     4921 tgataacaat tcttgaacga ttcggaacaa gtgatcctca atggaactag atagttttac
     4981 atcttctaca tcccatttca gcgaaatctg gattttattt tgcaattcct tcaataactg
     5041 ctcgatacct tgctttaaac ttttctcttc taaattgact ggacgtagat gaagaagcag
     5101 cgcacgcatc tcagactggg aagcgttgat gatctctgct accattttca actgcttgcg
     5161 aaacatttct ggtgtctcgc ttttttctgc ttgctccgtc aatgccgaca tcatcatcat
     5221 tgctgcaaac aattgttgcg aaactgaatc atgcagctca cgggctaatc gatgtctttc
     5281 taattctaaa atttcttctt tggtttgtcc atctacatat tggggacggc tcgttacaat
     5341 ttgtaattca ctagatactt caatcatttt ttcttttatc ttagtgattt ccttatcaat
     5401 atctttgatg tataaatcgt ccgatgcact tgggatcgga agaagcaaca atttggattc
     5461 ataattgcca gaagaaagtg cacgcagctt ttcttcgatt ttcccgtatt ttgtcttttg
     5521 aataagtgat aataatagaa agacaattaa tcctacacct aaggaaatgg ctaacaaatg
     5581 gaagataagc ggaacatata ataaacgtgc acgcagtaat tctccccacc aatgactttg
     5641 attgctggca taaaaataag tgaaaagtga aaatagaata ataaggaaag ccgcaatccc
     5701 cgaataaaca gctagcatcg ctctggatat ttttcccatc atattcgtat cacctcgaca
     5761 tctccaatca aggtattagt aataatcttc aatctacgag ggttttcatc ataatcactg
     5821 ctgtagattt tgatttgttc atttttcaaa gaaaattgct cttcttcaaa caagacattt
     5881 cctactaatg tcgcatgctc taatcggatg gctacgccca aaggtactaa gatccgtgtt
     5941 ctgcctatcc cttttcttac aatcacaata ttatcatctt ttggcaaaag cgtattgccc
     6001 aaatctatga tagtatcacc agaaatgacg gcgatgttga tatcgtccca ttcatagacg
     6061 tcgtttccaa tacgttggtt accgaataat tgctgtcttt tccgctcatt attatgtgtt
     6121 tttaattgct ctgtttggat catcatgatc tgtttctttc gccaaaaagc atttttggtt
     6181 aaatctatcc ctgaaatctc tacacctttc agaccaataa ataaaacggc aaatacagcc
     6241 atcatccata aagcaggact gttgactaag ctaaaaaaaa tgaccaaact tcctaatatc
     6301 aattgaaaat tttgaaattt agtacgagga tgtttgcgca tagctagata tatattgaaa
     6361 ataccgaata tcactaaaag caaaagtcca gtattgttta cgatttgcca aacagcaaat
     6421 aacagcagta gtgcttcaac taccacaaaa aaacgccaag aactattcat cgctctttct
     6481 ccctcctgac acattctcat ttcatcatac ctattttttt aactgttggc tataggactt
     6541 tagatgtaag atcgtttgtt gaaatctaaa atacacaaag agaaaaagat ctagagtgta
     6601 tcatcagtaa gatgaactct ctagatcttt cagtcagact acgcttgttc tactttgacg
     6661 attttaacta acatatcgcc acctggagta gcaatagtta cttcatcatt tttacgtttg
     6721 ccaatcaatg cttgcgcaat aggagaatca ttggaaattt tgccagaaaa cggatctgct
     6781 tcagcgcttc cgacaatcgt gtactcttct tcttctccat ctggtagttc gatgaatgta
     6841 actgttttac caattgatac ctcatctgca tctacaccgt cgttatcgat gatttgagca
     6901 aaacgaatca tattttcaat tgttgtgatt cttccttcaa caaatgcttg ttcatctttc
     6961 gctgattcat attcagagtt ttcggaaaga tctccgaaac cgcgggcaat tttgattcgt
     7021 tcaatgattt ctccgcgttt gactgttttt agttcttcta attctttttc taatttttct
     7081 ttaccttcta gagtcattgg ataaactttc tctaccatgt gtaacgtctc cttttttcga
     7141 taaaataccg caaaaggtaa tcttttgagg aatatagtcc aaaaaagatt accacgcttt
     7201 tcttcaaatg taaatgtttt tctaaaaatg atcaatgtct agagcctgtt tttctaagaa
     7261 ttatttacat atttttccac taattccata tgttcttcat aagttttagc gaaataaaca
     7321 tttccagtgc tcgtatctgc tacgaaataa taatagtcat tttctgctgg ttctaatact
     7381 gccttgattg cctcttcgct tggattgtca aaaggtccag gtccatagcc ggtattggta
     7441 tacaaattat atggtgaatc tacagctgta tcttcatacg tcactaattc tttgtgttca
     7501 cctaatgcgt ataaaacaga gatatctgac tgaagtggca tatttgcttt gattcggtta
     7561 aagaagactt gtgcaatgtt tttacgatca ttttctttta cgccttcttt ttcaactaga
     7621 gaagctagag ttaaaacttc ttgtacagtc aaatttttct gcttgatagc tgaataatag
     7681 ttttgcatga ccgtattcgt cttgttcacc atttcaatca ctagatcctt aagcgaagta
     7741 ctcgtgtaat aatcatatgt ggctgggaat aagtaacctt ccaaacggta ttttgtgttc
     7801 tcagctttag atgcactttc caacagttct gggaattttt ggtgcaactc gttaaagaaa
     7861 gtctcatcat tcactaaatc taaaaattct ttcttgtctt taccagttac cttcgctacc
     7921 cgttcagcaa tctgatcgat atcgtaccct tctggaatgg caattttccc atctgcaact
     7981 tttgttggct cagaagtacc gccttcttgc aactgtttgc caatctcgtc aagagtcata
     8041 ttaggcgcta actgataata gccagcttga aagccagtta aatttttaaa ttttgtataa
     8101 taattgaaaa caatcccgct tttaataata ttgtccttct ccaaaatctc accaatctgc
     8161 ttgttagaag agccactcgg gatctcgact tggactaatt ggtcatctga tttatctaaa
     8221 ggcttcaacc cgctatctac ataacgatac acggtaaatc caagaaatcc gccaataatc
     8281 aataatgtaa gagcaatcac taaaatgatc ttcctcacaa tcttatcttc ttttttacgc
     8341 agatttctcc gcttgtcgat tatatctgct gtagctgcct tactggcttc ttttttatct
     8401 ttacttgggt gttgtttgtt tcctgacaaa acaactcctc cttactatca agttcgtaac
     8461 tttattcatt atacatgata tttgaattgg cgtgaatgat tttatttaaa tattctatca
     8521 aaaatgta
//
LOCUS       DAHHZJ010000122         8476 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.123, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000122 DAHHZJ010000000
VERSION     DAHHZJ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.123"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            259..1782
                     /locus_tag="NBL02_002157"
     CDS             259..1782
                     /locus_tag="NBL02_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HCD2572834.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            2019..3359
                     /locus_tag="NBL02_002158"
     CDS             2019..3359
                     /locus_tag="NBL02_002158"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HCD2572835.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            3512..4249
                     /locus_tag="NBL02_002159"
     CDS             3512..4249
                     /locus_tag="NBL02_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HCD2572836.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(4414..4635)
                     /locus_tag="NBL02_002160"
     CDS             complement(4414..4635)
                     /locus_tag="NBL02_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HCD2572837.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            4666..5697
                     /locus_tag="NBL02_002161"
     CDS             4666..5697
                     /locus_tag="NBL02_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD2572838.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            5684..7087
                     /locus_tag="NBL02_002162"
     CDS             5684..7087
                     /locus_tag="NBL02_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCD2572839.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            7153..7788
                     /locus_tag="NBL02_002163"
     CDS             7153..7788
                     /locus_tag="NBL02_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCD2572840.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            7837..>8476
                     /locus_tag="NBL02_002164"
     CDS             7837..>8476
                     /locus_tag="NBL02_002164"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCD2572841.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAA"
BASE COUNT         2806 a         1447 c         1810 g         2413 t
ORIGIN      
        1 caatcaattc tttgcaaatg tgaaattctt ctccttttaa aagacgaaaa tgtttgttta
       61 taaaaaaaag gaaacgctac aatgaaaaag aagaaagctt taaaaacctg aaaaaagagg
      121 agggaatagg gaaaaacgaa ctttttgaac aaaagtttta caaaatgcgt gttattattt
      181 ttgttctgat actctttttg ataaaataac attagagcca aaattgttgt ctctaacgga
      241 aaagaaagga agaagaagat ggaacaaaca aaaaagaaaa aatttcaaat gccttcagcg
      301 tatacggtct tatttattat tattgcagta atcgctgtac ttacttggtt tataccagct
      361 gggcactata gtgtagacga tgcaggcaac atcgtcgctg gatcatatga acgagtacaa
      421 caaaatcctc aagggctgtg ggatattttc atggcgccaa tcaatgggat gctaggtgta
      481 ccagcggggg agctaactaa agaaacacct gctgcaatcc caatctcttt cttcattttg
      541 gttgttggtg gatttttagg tattatcaac aaaacagggg cactagatgc cgggattgct
      601 tcagttgtga agaaattcaa aggaaaagaa aaaatgctta ttccagtatt gatgctgtta
      661 tttgcattag gcggctctac ttacggaatg gcagaagaaa cgattccatt ctatgcttta
      721 ctgatcccag tcatgatggc tgtaggcttt gatacagtag tagctgtagc aatcgtatta
      781 gtagggtctc aagtaggctg tctagcttca acggtaaatc cttttgcaac aggcgtagct
      841 tcacaagctg ttgggatttc gatgggtgaa ggtattggtc ttcgattctt gatgttcgtt
      901 attttgttag ctatttctat tgtatatgtg tatcgttatg catcaaagat cgaaaaagat
      961 ccaacaaaat cattagtata caaccaacgt aaagaagata tgaagcattt cgatattgaa
     1021 gcaatcggtc gtcaagaagt aatcacgaaa aaacaaaaac atgttaatgt attgttcttt
     1081 attacttttg gtatcatgat catcagtttg attccttgga caaccttgaa tgcgaatttc
     1141 acgatttttg aatcattgac aaactggtta acaaaccttc cggtactagg tacggttcta
     1201 ggtaaaagca tgcttccatt aggtgactgg tacttcccag aaattactat gttattctta
     1261 gtaatgtcca tcgtagtagc aatcacttat ggcatgaaag agtctgaatt tgtctctgaa
     1321 tttttagctg gtgcttctga tttgatcggt gtagcaatcg tagtagctgt tgctcgtggt
     1381 atccaagtgg ttatgaacaa tggattaatc acagacacca tcttacattg gggcgaacaa
     1441 ggattaagtt cattatcttc aagtgtattc atcattatta catttatttt ctatatccct
     1501 atgtcattct tgattccttc aacttccggc ttagctgctg caacaatggg aatcatggga
     1561 cctatgggtg aatttgccgg cgtcggtaag gatttagtaa tcactgctta tcaatctgca
     1621 tctggtttag taaacttaat tacaccaaca tctgctatcg taatgggtgc tgtagcaatt
     1681 gctcgtttcg atgtttcagt gtggtggaaa tttaccggta aattaattgc gatattattt
     1741 gtagccatct gtgtgatcct aggtgtagca gcaatgttct aaacctagac aaatacaggt
     1801 acaaggtcta tcataagaat gaagaaaccg ataaacggct atcgataagc acgtggacaa
     1861 gtcaatagct ccattcagtg agtgtttttt ctttcatcat ttgttaagaa cattctgctt
     1921 gctgatggag caggcttaaa acgtcattat ttgagaagaa gaggttgtgt cggaggaatt
     1981 tgacatgacc ctttttaaca tattaggagg ataatatcat gaaaaaattt gtgacagaac
     2041 aacatcatga acaagcatta gaatcattga gcgaattgat ccgaattcct tccgtactag
     2101 atgaagcaga ttcaggacaa ggacatccct tcggtaaaaa agtgattgaa gctttggata
     2161 aagtattgga aatcagtgaa aagctaggat ttagaacatt taaagatcca gaaggctatt
     2221 acggttattc agagattggc tctggagatg aattattcgg tatcttgtgt catatggacg
     2281 ttgttccagc aggggacgaa aataattggg aaacaaaacc attcgatcca actataaaag
     2341 acggctggtt agttggtcgc ggatcccaag atgacaaagg tccttctatt gcggcaatgt
     2401 atgccgtcaa agctttgatt gatgctggcg tggaattcaa gacacgtatt cgttttatct
     2461 ttggtacaga tgaagaaaat ttatggcgtt gtttagaaaa atataacgaa aaagaagaag
     2521 gaattacgca aggatttgcg ccagatgctg aatttccttt aatttatgca gaaaaaggct
     2581 tattgcaagc ttacttgaca ggaccaggaa cgaacgagtt ttctgtaaaa gctggaggag
     2641 cgttgaatgt tgttcctgat gcagctcctt actctggaga aaagctttct gaagtaaaag
     2701 aagcattgaa aaaacacgaa ttcgatttcg aagaacaagg tgaaggcatc gtcgttcttg
     2761 gtaaaagtat ccacgcgaaa gatgcagcac aaggagtgaa tgctatttct cgtctggcaa
     2821 ttgctctttc agaagtattt gattttggcc caatcaattt cttaggaaaa ttagttcaag
     2881 aaaatgcaac tggtgaagct gtcgtgggca aaacagaaga tgaacaatcc ggcgagctaa
     2941 cgatgaactt tgcaagtttg gaaatcacac cagaacaaac aaaaatcggt gtagatatga
     3001 gaatccctgt tacattcaag aaagatgatt tagtagctaa attaactaaa acagcagaaa
     3061 aatatgggtt gacttatgaa gaatttgact tccttgattc attatacgtg ccactagaca
     3121 gtgagttagt gaaaaatcta ttggggacat atcgcgatat tactggagac atgactgaac
     3181 cgtttgtatc aggcggcgct acttttgctc gtacgatgaa ccagtgtgtg gcattcggtg
     3241 cgatgttccc tgatactcct gactttatgc accaggcgaa tgaacgatgg gaattaagta
     3301 gtatgtataa agcaatggaa atttatgcag aagctgtgta tcgtttatgt gctaaataaa
     3361 aataaacata caaaaagggc ggatattcaa atggatattc cgtctttttt tatttgatgc
     3421 tggaaagttt gcatttctct cttatttttt taaggtataa tcatacttga ttaagaaaaa
     3481 ggggtcactc agatagaaaa ggtgggagaa agtgaaaata tttagacgaa tactaattgg
     3541 cttagtcatc attttaggat tactcggtgg agccggttat tactatatac agcaaaatac
     3601 gtacgaacca tcgtcagaag cgcaagtgtt ggtaaaagaa ggaaaaaaca aagaagatta
     3661 cttatttttt gaaggagaga aaaagaatca tggaaagtct ccattactta ttttttatcc
     3721 tggtgctctc gtagagccag aaagttatag tatttgggcg caagatttag caaaaaacgg
     3781 cttttcagtc gctattgtca aaatgccact tgatctagca gtgatgggcg gaaacaaagc
     3841 ggataaggtc aaagaagatt ttccaaattc cgattatgtg atcggtggcc attcattagg
     3901 tggcgtaatg gcaagtcgtt atgtcaataa gaatcattca gacaaacatt taaaaggggt
     3961 attcttcatg gcaagttatc ccgacaaaaa aggaacattg acagaatcag ctgttcctgt
     4021 tttatcgatc acggcgacaa aagataaagt attgaatcag gaagcttatc aagaagctaa
     4081 gcagtattta ccggaaagaa cagagtatgt atcaatcaat ggcggaaatc atggaggttt
     4141 tggcagctat ggtgagcaaa aaggagatgg agaggcagaa atttctaacg cagaccagca
     4201 aactgtgatc gtcaatacat tggaaaactg gctgaaaaag ttgaaataat ataaatgggt
     4261 cctatagggt ccgtgaaaaa actagtcaat taactataga tagcaaatga atctttcaaa
     4321 ataagtcggg atttcacgga acaagagaaa cagatcccgg cttattttca agtttagaga
     4381 acttagtgta cgtttttaaa gtttcgttta tatttatatt ttattttcta ataaaatcgc
     4441 tcgaactgga catttttgat acgctttttt tacatatgtt tcttcttccg gcatcacgaa
     4501 ctgttgatga gcctctggtt cttgggcaaa taaaacgatt ccttcttcat cataatcgaa
     4561 aatatctggc gcttcgattt gacaaagacc gcatgcaata cagcgttcag gtacaagttt
     4621 acatagtagt gacatttcta accatcctaa cttaaggaga ataatatgat tgtttttatt
     4681 ttatcgctat tttcatcagg acacaagctg cggattagtt ctctttacca actccttgtg
     4741 ggaaaacgaa cgacttcagt acttatttat ggatttaccc acgaactttt gtttatccat
     4801 aacagttttc cagaattgaa gcaggataag ttttatcaaa ttttgcaaaa aattgcgcag
     4861 caaggttgga tcgaaataaa tgaaaatgaa gcaaagctaa cgcctgcagg ggctgatatg
     4921 ttgtccgaac atcaaataga acatactggt ttgcgttttg accgctatgg cagaacaggg
     4981 gaaactagtt ggcggctcat taaatttgct gttcaagtaa tttctaatct ggcaacgggt
     5041 attcaagact atcttccagc agagacaagt cctttttata ctttccaact aaaaaaatgg
     5101 cttagtgaaa gccaattgcc acgtgaaatt ttaattgata ctgcgtatga gagtttggtg
     5161 cagctgtttt cggagatacc agaagaagca gcagattttt tagctaacca attatctgga
     5221 aatgagcgaa cagggctgct tccttatcag ctggcaaaaa acaatgatga gtcagaggtt
     5281 tatctgcaac aaagcagatg tatccacctt ttgttagcac aaatcgaaaa acggccagat
     5341 tcgctttggc acgctttgat cgattcatta ttacagcaaa atttcaatca aagcatgatg
     5401 atcaccaagc agatgtttat gaatggtcag acaattgacc agatcatggc aatccgtcat
     5461 ttaaaaagag gaacagtaac agatcatctt attgagtggg ccttgttttt tgatgatttt
     5521 ccatacgagt ggattctctc tgcagaaaca atcgaacgtt tggaaccaaa taaggattcc
     5581 gtgcgagagt ggcgctttag cgaatggaat gtagatggac aattagatta tggagaattt
     5641 cgtttgtatc aaatctatct attgagaaag gaagcgatac agaatgttaa taaatgaatt
     5701 aaaaaaatat tttggatttg attcatttcg tcccggccaa gaagaaatta ttcaggcgtt
     5761 gcttgatgga gaagatactc tcgctatttt gcctacagga acgggaaaaa gcctttgcta
     5821 tcaattgacg gggtatttaa tggaaggtct ggtcatcatc gtgtcaccgc tgctttctct
     5881 aatggaagat caagtgacgc aattacaaaa acgaggggag aaacgagtag ctgctttcaa
     5941 tagtttactt tcgcaatctg aaagaagata cgtcctccgt catctatcgc aatataaatt
     6001 tttgtttttg agtcccgaaa tgttgactaa tccttctctt ttagagcatc tgaaaaaaca
     6061 aaatattgcg ttatatgtag tagatgaggc tcattgcgtg tctcaatggg gagttgattt
     6121 ccgtccagaa tatcaacagt taggaaaaat ccgaaagctt ttaggaaacc cggtcacact
     6181 ggcactaacg gcgacagcaa cagatttagt agcaagagat atacgtcatg tactatttga
     6241 tcgaccacca aaagagatca gacaatcagt caatcggcaa aatatctcgc tttttgttcg
     6301 gaaaacgcag caaaaagaac aagaacttga acacttcatg gaaagagcac aaggagcggc
     6361 aatcatttat tgtgcaacaa aaaaagaagt cgaacgattg tatcatttat tcagggaacg
     6421 ttttacagtt ggatactatc acggtggtct tgatgcagct caaagaagac aacttcaaca
     6481 gcagtttgta aaaaatcagc tgcagtttct cattgctacg aatgcatttg gtatggggat
     6541 cgataaatct gatatccgtt atgtggtcca ttatgatttg ccagacagtc tagaaaacta
     6601 tgtacaagaa atcggtcgag caggcagaga tcaggaagaa agtgcagcaa ttctttttta
     6661 tcaaagtggg gatgaacgta tccattattt ctttaatcaa ctttctagag aacagcgaca
     6721 aagttttgag ctttatttgg aatatgccac aaagcaagct ccctttgatg agctgcaaaa
     6781 aaaatggatg gagctgatcc agcagtctga aaaaccagaa aattgggtgg aacggctcaa
     6841 acgtcaggaa aaagaaaaag aattccgcct gcagcagatg cttcgctata ttaatgaaga
     6901 aaactgccgc agaaaattca tcttagctta ttttggcgaa aaactctccg aaaagccgca
     6961 gaattgttgt gatattgatg gagcaaaagc agaacttctg ccaaagagga agcttttttc
     7021 taagactgaa caacttcatt gggaaacgat tttactaaat ttatttaaaa aaaacatgga
     7081 acattgattt tttaggacaa gacacttttt cctatggtat aatacacacg atagagttta
     7141 ggaggtaaaa cagtgagcag aaaagataga catcaatcta cagaaaataa cgaaacacaa
     7201 gaaccatggg aacaaccaat ctacgataca gatgatgaag tttcatcaag aagttctcag
     7261 agacaacaaa agaaaggaaa tacattattt ctttcgctat ttttgatttt actggtctta
     7321 tgtatcgcta ttccagcagg tgcttatttc tggatcagaa atggatcaag caacaatgca
     7381 acagcagcaa cttcttctga aacttcgaca tcagtagtag aaagttctac aagtacaaca
     7441 aaagaggagt caactgagac ttcaacagta gaatctagta caatcgcgga agaatctact
     7501 gtgtctcaag atacaggtgt aacgtcagaa agcagtattg cagcagagac aacaccgact
     7561 tcctcaagtg aggcaactta tcaagcaggg gattcaagca gcaatgcagc agaaacaaca
     7621 ccttcttcat cagctgcagc cggttcaaca acgactgttc aagcaggcga aggtccaaaa
     7681 caagtggctg agcgtgcagg gatcaccact gatcaattgt ttgaattgaa tggcttagat
     7741 ccaaacaact ttatgcttta ccctggtcaa gaattaagaa ttaaataata atagccgcgc
     7801 tttgcggctt tttttctgag aaaaaggagt tatatcatga cacaaatcag tattgcaatc
     7861 gacgggcctg cttcatctgg gaaaagtacc gttgccaaga tattagcaaa agaattgaat
     7921 tatatttata cagataccgg tgccatgtat cgcgcagtaa cttatttagc tattacgaac
     7981 aatgtatctt tttcagatga agaagggcta gtttcgctga tcaaaaagta tcctatctct
     8041 ttttcgcaac aagaagatgg acagcatgta ttcatagcat caaaagatgt gactttagaa
     8101 atccgtcaac cagatgtcac aaaagctgtt tctgaagtct ctgcacatgg aaaagtcaga
     8161 gaagaattag ttgctgtcca acgaaaaata ggggaacgcg gtggcgttgt catggacgga
     8221 agagacatcg ggacagctgt attaccgaat gcagaagtga aaatcttttt agtagcaagc
     8281 gtaagtgagc gtgcagaaag acgtttcaaa gagaaccaag aaaaaggaat tccgactgat
     8341 cttgaaacac tgacgaaaga gatacaggaa agagaccatt atgattcaac aagggacgtc
     8401 tctccgctga agcaagcaga ggatgctgtt cgtatcgata cgactggcaa atcgattcca
     8461 gaagtcgttg cagcaa
//
LOCUS       DAHHZJ010000123         8450 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.124, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000123 DAHHZJ010000000
VERSION     DAHHZJ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.124"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(31..561)
                     /locus_tag="NBL02_002165"
     CDS             complement(31..561)
                     /locus_tag="NBL02_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HCD2572842.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(660..1010)
                     /locus_tag="NBL02_002166"
     CDS             complement(660..1010)
                     /locus_tag="NBL02_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HCD2572843.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(1167..2696)
                     /gene="ahpF"
                     /locus_tag="NBL02_002167"
     CDS             complement(1167..2696)
                     /gene="ahpF"
                     /locus_tag="NBL02_002167"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCD2572844.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(2706..3269)
                     /gene="ahpC"
                     /locus_tag="NBL02_002168"
     CDS             complement(2706..3269)
                     /gene="ahpC"
                     /locus_tag="NBL02_002168"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HCD2572845.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(3413..4075)
                     /locus_tag="NBL02_002169"
     CDS             complement(3413..4075)
                     /locus_tag="NBL02_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCD2572846.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            4259..5905
                     /locus_tag="NBL02_002170"
     CDS             4259..5905
                     /locus_tag="NBL02_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCD2572847.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            5896..6978
                     /gene="uxuA"
                     /locus_tag="NBL02_002171"
     CDS             5896..6978
                     /gene="uxuA"
                     /locus_tag="NBL02_002171"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCD2572848.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(7086..>8450)
                     /locus_tag="NBL02_002172"
     CDS             complement(7086..>8450)
                     /locus_tag="NBL02_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD2572849.1"
                     /translation="NLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESVSYQIL
                     TSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSFSGKEV
                     QIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKLYFLLK
                     ICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILARLSIT
                     KIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAFARNKL
                     YNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILEKENVQ
                     WMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQNDFMENC
                     NIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQSKKEN
                     QVTS"
BASE COUNT         2703 a         1558 c         1532 g         2657 t
ORIGIN      
        1 tatacggtct attttaataa ctaaaaatga ttaacgtata gtcatttttc ttattctgat
       61 gaaattgata atctcctgga tgcccatgaa aatcaaaata aagccgaata tttggaatag
      121 aagcatcaaa cttttgaatg gattgaacaa taagacgaat ccgccaatca acaacagaat
      181 actactataa acactccaac ttttagcttt tgtattcatg ctgaagaaca actggaacaa
      241 gctgttgatg ataacagcta atccaagaag aatcggcagg atagaaacga ttgcaggtgc
      301 aaaagcatag acgatcagag ctaagatcag gaaaaaaaga ccactgaaca gaccgatact
      361 ccaagaacct gttttccttt taaaacggta agcctcaaaa agattcaaca acccaagcaa
      421 aacaagatag gcggtgatca gataaccgat aaaatgaaaa acagcagaag ggtttagcac
      481 aatcgcaatc ccagaaataa tataaacagc agctcttaat aaggcgtatt tttgaatatt
      541 ttgtttaatc gtctcaaaca tataagaaaa cctcctttga cttattttaa cgtgaactag
      601 tcattttacc aaattaaatg ttacaaaatg agcgaaggag aagaatatct ctggaaaaat
      661 caacttctga ggatcttttc catttttttg ccttttgcta gctcatcgat cattttatct
      721 aaataacgaa tttctttcat caagttatcc tcgatgtctt ctactcgtac accacaaaca
      781 acacctgtaa tcaaggaacg tgaaggattg gggacagggg cttccgcaaa aaattgttcg
      841 aaagtcactt gtttttgtag ttgatattct agttctgttt gggaataacc cgtcagccaa
      901 caaatgattt catctacttc ctcttttata cgatcttttc tctccgcttt tgctacatac
      961 ataggataaa cgcttgcaaa acttgtcgta aaaatacgat tttgcgccat aatcagtcac
     1021 ctttcttttt ttcaaattat ttactactta tagtatataa gaaaaagaga agaaaaagcg
     1081 attattcaaa aaaacaaaaa ggattgtgac aaaattcttg tcaccatcct tttttccgct
     1141 ctctgacttt ttacaatctt catagctcaa gtgcgcatca gataatcgaa tgctcctaaa
     1201 gctgcagtag ctcctgaacc cattgaaaca atgatttgtt tgtatggact atctgtacaa
     1261 tcaccagctg cgaaaacgcc ggggatattc gttgcgccat ttttctcggt cactatttca
     1321 tttcttggat tcatttcgat cgttccatct agccaatccg tattagggag taagccgact
     1381 agaatgaata ctcctgaaac agctagtgta tgcatctcca tcgtacggcg atcgatatag
     1441 gagagagatt ctacctgatt cgtaccgctg atttctttcg tcgctgcatg tttgataaca
     1501 gtgacattcg caagagagac tagtttatcc tgtaagattt gatctgcttt taattctggt
     1561 aagaattcta gaacatatac atgttttgct aacccagcca aatcgattgc tgcttcaaca
     1621 ccagaattac caccaccgat aacagcaatc tcttttcctt taaataaagg gccgtcacag
     1681 tgtgggcaat aagcaattcc tttattttta aactcttttt cgcctggtac attgatagaa
     1741 cgccagtgcg cacctgtcgc gatgatggcg gtttttgctt taagtgatat accgttagct
     1801 aagccaatat taataaaatg ttgctcttta tttaaggaaa cggcctgttg attcatcata
     1861 atgtcgatag ggtaactacg cacatgactt tctacttgct gcatcaattt tggaccttct
     1921 gtgtaagagg taccaatcat attttcaatc cctagtgttt caacgacttg tccgccgaaa
     1981 gaatcagcga caagtcctgt acggattcct ttacgtgctg catagatagc tgcactacta
     2041 gctgctggtc caccgccaat tactaataca tcataaggtt ctttttcaaa caactcttct
     2101 tcaatcaaag ggccagtgat tttttcaaca atttgttcga tcgtcatgcg tccgctacta
     2161 aattcttctc catttaaaaa cacagttggc actgccatga tttttttatc atcgatttct
     2221 ttttggaaca tcccaccttc aatcatcgta tgagtaatat ttggatttaa tacagctaat
     2281 atattcaatg cctgtacaac gtcggggcaa ttatgacaag tcaagctaac ataactgtca
     2341 aaatgcaatg tttgtgttat ttttttgatt ttaagactca aagattcact gattttcggt
     2401 gcccgtccac taatttgtaa catagccagt aaaaaagagg aaaattcatg accgaggggg
     2461 ataccagcaa aaacgattcc actttctttg ccaactggat tgatagaaaa actaggtgtg
     2521 cgcatcgaat ctcttttttc ccaagagatt ttaggagaca tttctgctat ttccttcgta
     2581 aattcagcta gtttttgact attttcacta ttatccaaac taacagaaaa aacaatagga
     2641 gattcaatca gtgctaaata ttgttcgaga ttttgtttag tttctttgtc taacatatcc
     2701 accacctaaa tctttccaac aagatcaaaa cttggtttca atgtttcgcc actttctttc
     2761 cattttgccg gacaaacttc acctgggtgg gtacgtacat attgagcagc gcgaatctta
     2821 tcaatcagtg cactagcgtc acgaccgatt ccatctgcat tgatttccat agcttgaacg
     2881 attccgtctg gatcaataat aaatgttcct cgctgagcta atccttgctc ttcatccaaa
     2941 acatcaaaaa tacgagaaat ctgatgagac ggatcaccga tcatcacgta ttcgatttta
     3001 ccgatagcat ctgatgtatc atgccatgct ttgtgagtaa agtgggtatc ggtagagcaa
     3061 gaaaagactt caacacctaa agatttcaat gtactgtatt gttcttgtaa gtcttctaat
     3121 tcagtaggac aaacaaaagt aaaatcagct ggatagaagc aaagaatact ccagttcccc
     3181 ataagatctt tttcactgac ttctaaaaat tctccttgat gataagcatg tgcattaaat
     3241 tcttcgattt ttttcccaat caatgacata gtaaaacctc caataataat tatttattta
     3301 taattattgt aatttagttg gagttgcaaa gtcaatggat attttgttat ttaataaaga
     3361 ttactcaaaa acgaaggtta attataaaaa tagaagaggt cgcaagaaaa tgctactgct
     3421 ggaaatactc gggataacgt tgagtaacag tttcctgttc tttcagcatc atgtgtaagt
     3481 gcaatcccgt ttgaaatcga gcttcttctg gttttttatc aactaaagcc ttgagtaaat
     3541 cataatgttg ttccatgatc gtttcccatg gaagcccctg aatggtcact cgtaaccagc
     3601 ggaaacgttc taaatgggta ttatggtcat ctaaccagtt ccaaacttct tttcttcctg
     3661 caatttcgaa acaagcttca tgaaataaat tatctgcacg gaaaaaagcg cgttcgtcat
     3721 ttattttgac tgctttttct tgttcttcta aaatcgtttc tagcactatt cgtgtttgtg
     3781 tgtccaattt ggaacaacat tcaatcatga tttgctcttc caactgttct cggacaaagc
     3841 gagcattttc tgcagaaaca agattaattt tggaaacata cgtaccactt tgaggtatcg
     3901 tatacacgag ttcttgctga cgtagttgaa tcaatgcttc gcgaatggga gtccggccga
     3961 tagcaagatg ttcctccagt gccttttcag atattttcct tccaggttct aagtcagcat
     4021 agataatttg tttacgaatc gcttgataag cttgtgattg caagctgctt ttcatcttta
     4081 ttcctccaaa atcatttcta tgaacgtagt ataacagaag aggtctgaaa aacaatctgg
     4141 tatgtcacat taactagtaa atttgtataa atagtaaaat tttggttgac atagcgattt
     4201 ttattgtata aaattcactt gaatacaaaa actaatatgt tagtttagga ggggaaaaat
     4261 gattgattta aacaactgga ggaactttat ccctgaatta tctcaaaaag aagtagaaat
     4321 acctcatttt gaccgtgcat cattaagaga aaatggacaa aaaaatcctg tctggatcca
     4381 tttcggtggc ggaaatcttt atcgaggatt tcatgcagaa atcgctcaac gtttagcgga
     4441 tctaggcgaa ttgtccactg gtgtcattgt ttgtgaaaca ttcgatgaag aaataattga
     4501 aagggcttac caagattttg atgatttgat tcttgaagtg gtcatgccaa gcagtggaga
     4561 gttcgtcaaa cgtatattag ctgcgacagc tgacagtctg tattgtcatc cagattgttc
     4621 tgaacacttt gataagatga ttcgacactt tgaaaatcct agtttacaat tagttacttt
     4681 tgctattact gaaaaaggtt atcgattgaa agatacaaat aataaatata taggaccgat
     4741 tgcagtcgat ataaaaaatg gtccaaagaa gccatcacat acaatgagta ttcttgctgc
     4801 gctactttat aaacgttttt tatctggagc ttggccgatt gcacttgtct ctacagataa
     4861 tttttcacaa aacggtagtc aattacgtac ttctttgcta acgatcgcaa aagaatggca
     4921 aaaggaaggt tatgtacaaa atacatttat ccaatactta tcgaatgaag aaattgttgc
     4981 gtttccatgg acgatgattg atcgtatcac cccgaatcct tccttgtcag ttagcaaaat
     5041 gttggcaaaa gagggctttt caaatatgtc gattttccaa accgcaaaag gaaataatat
     5101 tgctccattt gtcaatacag aggacatcca ttatttaatc atggaagatc attttcccaa
     5161 tggacgtccg aaattcgaaa aagctggggt attcttgact gatcgtgcaa cggtagataa
     5221 agcagatacg atgaaggtga cgacttgcct taatcctttg catacgtctt tagctattat
     5281 gggttgttta ctggggtata ggagcatagc tgaggagatg aaagatccag atttatcgct
     5341 gctagtttct gaaatcgggt acaatgaagg cctcccagtt gtggagtctc caggtatcat
     5401 tgatccaaag atctttctgg aagaagtaat tactgtgcgc ttttctaatc caaatattcc
     5461 tgatatgcct cagcgaatcg ccacggatac ctctcaaaag gtggcgattc gttatggtga
     5521 gacgattaaa aaatatatgg aaatagaagt taaagacccg cagacacttg tattcatacc
     5581 attgactctc gctgcttggc ttcgatacct tattggcatt gatgatcaag gaaaaccatt
     5641 catcccgagt ccagacccac tattgacaga ctttcagcat aagctttccg atattcaact
     5701 gggtacgcaa gatacatcac atattcatga gaaaatggct cctattttgt caaataaaga
     5761 aatttttggt gtagatcttt accaagcaga gcttggcaga aaaatagaag aaatgttcat
     5821 caagatggta gcaggaccga atgccgtacg aaaaacatta caggattact tacaagaaca
     5881 tggaggaaat ttatcatgga aatgactttt agatggtacg gatcacagga agaaaagatt
     5941 accttagatg aaatcagaca gattcctgcg gtcaaaggga ttgtaggcac attgatggat
     6001 ataccagtag gagaggtatg gcccagggag aggatcaaag aactaaaaga agaaattgag
     6061 tctcgtgggt taacactaaa agtaattgaa agcgtcaatg tgcatgatgc cattaaaatt
     6121 ggattacctt cccgtgagca gtatatcgaa aactataagc agacaatccg aaacttggca
     6181 gaatatggaa tagaagtcat ctgctataat tttatgcctg tatttgactg ggtgaaatct
     6241 gaccttgact accgcttaga agacggctca tccacgttag cttttatatc tgatgatatt
     6301 ccagatgatc caagagaaat cgtcgcgcgc atcgaacatt ccagcaatga atttgaattg
     6361 ccgggatggg aacctgagcg cttagctcat atcaaatcct tgcttgaagc ctatgcatca
     6421 gtagacgaag aaaaacttcg agaaaatttt gcctattttc tccaatcaat catcccaaca
     6481 tgtgaagagg taggggtcaa aatggctgta catccagatg atccgccata ctcgattttt
     6541 ggattacctc gaatcgtaaa gaatcgagaa gatttagatt ggttgtgccg catagtagac
     6601 agcccttata acggaatcac tctttgtaca gggagtattg cagaagatcc agaaaatgat
     6661 gtatatggga ttttagcaga attttgtcaa agagatcgaa tcccatttgc acatgttcga
     6721 aatattaaat ttttagctaa tcgagatttc tatgaggcac cgcataaatc cgaatacggt
     6781 tcattagaca tgtaccgcat actaaaaaca atgtatgaca acggatttaa cgaatacatc
     6841 cgtcctgatc atggaagaat gatctggaat gagacaggaa gaccaggata cggcttatat
     6901 gatcgagcat taggtgctgt ttatctaaat ggattatggg aagcaatcga aaaaggaaac
     6961 aaaagtaaag ataattagtg aaaaaaactg taattttatt atgtaaataa gaaggatgtt
     7021 caaattcttg atacataaat ccggaggttg ttaataagca ttcggattta tgtatcttat
     7081 ttggtctagg aagtaacttg attttctttt ttcgattgat ccatgagtgc ttcttctttt
     7141 aattcaaaga tatattctga gatcctcata atcacttttt tccagtcttc tggtgtgatg
     7201 attcctttta aaatatagat ttttttgata tgagaaggca attgtttcaa agggcagtcg
     7261 ctgataacga tgttgcagtt ctccataaaa tcattttgta ttttgatcga ttcatttgaa
     7321 aaaatttgtt ctatcttaac gatcagatct ttactgatat aaaaatcacg cgtatagttc
     7381 acaccaatca caattcgcgg ctgttttttg tagcgatcat acaaatgtac taggtcttca
     7441 accatccatt gcacattttc cttttctaaa ataactggta acaagtgttg attttgttct
     7501 ctgaaatcca tataaaattg ttcaatggcg gtttggactt ctttcaacac gctatgttct
     7561 tgataaagca tgaaagtagg aaggggcgta tttggttgat tgaagttaag aaatccatta
     7621 taaagtttat ttcgtgcgaa tgccaatgag taaagaatct tttctttttc aggtataaac
     7681 aaatcaaata cagtacaaaa ggactccacc atttctcttg aataaagaac atgaggaacg
     7741 tttgcttttt ttaaaagttt gtattgctcc aagcttgttt cttccgattc gattttcgtg
     7801 atagaaagtc gagctaaaat attcactaag atgcgttgtt cgcgacaaat tggcagatta
     7861 ggtcgcaaaa tatctacaga gggatcaata aaaaagtcaa tatattcata ctctgtgaat
     7921 tctttttgta cgtctttcac agaagttgca ggaaaattct ctttacatat ttttaataga
     7981 aaatataatt tatctatcat accgctattt agtcggtcaa gtacttcttt tttaaaaaaa
     8041 gtattcatca taaaagccac tacttgcgga tcttcatcat aaaattctgt agcagtactt
     8101 gaaaagatgt tccaataaat atctaatgct gcatattgaa tttgtacctc ttttccagaa
     8161 aaagactttc tgccattatt agaaaaggag atagacaaac cgtacaatgc taaaaatgca
     8221 ttaatctttt tcattttcgt atacaaataa gaggcactat agtttgtttg ctgacttaaa
     8281 ctgataatcg aatttgtctg acagctgaac aaagaagtca agatctgata actgactgat
     8341 tcgttgcagt atcttccaaa tagacgataa tagcagtaag ccggatcaat tccttcaaaa
     8401 ttcaacttta aaaaaggttt ttgcttatca atatgcagtt ctgttagatt
//
LOCUS       DAHHZJ010000124         8284 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.125, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000124 DAHHZJ010000000
VERSION     DAHHZJ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.125"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            450..1313
                     /locus_tag="NBL02_002173"
     CDS             450..1313
                     /locus_tag="NBL02_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCD2572850.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1442..3064)
                     /locus_tag="NBL02_002174"
     CDS             complement(1442..3064)
                     /locus_tag="NBL02_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD2572851.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3313..3834
                     /locus_tag="NBL02_002175"
     CDS             3313..3834
                     /locus_tag="NBL02_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HCD2572852.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4020..5750)
                     /gene="spxB"
                     /locus_tag="NBL02_002176"
     CDS             complement(4020..5750)
                     /gene="spxB"
                     /locus_tag="NBL02_002176"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HCD2572853.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5915..6733
                     /locus_tag="NBL02_002177"
     CDS             5915..6733
                     /locus_tag="NBL02_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HCD2572854.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6836..7033)
                     /locus_tag="NBL02_002178"
     CDS             complement(6836..7033)
                     /locus_tag="NBL02_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572855.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7202..7780
                     /locus_tag="NBL02_002179"
     CDS             7202..7780
                     /locus_tag="NBL02_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HCD2572856.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7875..8012
                     /locus_tag="NBL02_002180"
     CDS             7875..8012
                     /locus_tag="NBL02_002180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572857.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2497 a         1479 c         1635 g         2673 t
ORIGIN      
        1 tcctcggtca caagtcacaa tattcaacaa acatgagaag ctgttttttg aaattgttcc
       61 ttgtgactcg gagctaaacg gcaggctcac aacctcttgg taaacggtgt tcaatcagct
      121 gaccctcggt attagctcaa aaaacggcaa aatatgagga actatttttt ccgttttttt
      181 ctctaatact caggtcaaaa cgctgatttc acaacctctg attcacggtg ttccagaagt
      241 agcttctacg gaaataagcc gaaatttcac aaaaatttga gaagcaattt tcgtaaattc
      301 cttcttattt cttgaagctg accacttctg tcacaacctc ttattttgat tagaacttgc
      361 gaaaataaac cggttaaagt gtgtctagcg caagagattt gctataatag acgataaggg
      421 taaaatgtaa cgagaagggg ttgaaaaatg tgtatcaaat tgtaacagat tcttgttgtg
      481 atctgccata ccatgtatta gcaaaagaaa aagttgattt tttatcaatg aatctccaat
      541 tgaatgatca agagttagta gatgacctcg gaaagacatt caattatgac cgctacatga
      601 atcagctgaa aaatggtgcg atgcctacga catcacaaat caatgttggc cgatatttag
      661 aatttttccg ttcttatgcg gaaaaacaga ttcctgtttt atatgtggct ttttccagtg
      721 gattaagcgg ctcttacagt agtgcgcttc aagcggttga attgctgaag gaagaatatg
      781 aagaccctga gatctatgtt tttgatacaa aagctgcaag tattggggaa gggcttttag
      841 tattggaagc tgtaagattg aaaaaagaag gtaaaagcat cgaagaaacc ctagagtggc
      901 tggaaagtaa ttatttgaaa gtccattcat gggtaacagt agacgatctg aaatatctag
      961 aacgaggcgg taggatttcc aaagcaagtg ctactcttgg cagtctattg agtgtaaagc
     1021 ctattattac ggtggataaa gaaggaaggc tgacgaatgt tgacaaagtt cgaggaagaa
     1081 acaagtcgat ccagacaatc gtcaatgaga cggtaaaaac aatcgttgca ccacttgatc
     1141 aaatcgttta tgtggcttac gctggggatc tagagagcgc ggagaaggca aaacggcttt
     1201 tggaagagca gataaagatg aaagatgtga agctttatcc cttaggacca acgatcgcca
     1261 gtcacacagg ctatggctgt atcgcgattt tttcgatggg aatcagtcga taaaagcagt
     1321 aactcattct gtaaaaaagc ccagaaattg attgtaacaa aaacagcgac tcttaaaaga
     1381 tagatgagca tcctatctct tagaggtcgc tgttttttta ttttttaagc taaataggcc
     1441 attatgccca taactcgttc aattgttttt gaatatctgc attttccaaa aattcatcat
     1501 atgtcgtatc agctcgatcg atcacacctt tatccgaaac ggcaatgatg cggtttgcca
     1561 atgtttggat aaattgatgg tcatgtgacg caaataagat tgagccagta aatgccatca
     1621 agccgtcatt caatgctgtg attgattcta agtctaaatg gttcgttgga tcatctaata
     1681 caagtacgtt agctttcgaa agcatcattt ttgaaagcat gcaacggact ttctctccac
     1741 cagataaaac attcactggt ttcaatactt cttcaccaga aaacaacatt cttcctaaga
     1801 agcttcttaa gaaggtgtta tcatcttctt ctttacttgc atattgacgc aaccagtcta
     1861 aaatcgttag atcagtatcg aattctttcg ttgtatcctt tggtaaataa gaattacttg
     1921 ttgtcacacc ccaacgtact gttccagtat ctggcgtaat ttcacccatg ataactttga
     1981 atagcgttgt tgttgttata tcattttctg cgacgaaagc gaccttgtct tctttgctca
     2041 aattaaaaga tatatcatct aaaattttct ttccatcaat cgttacactt acattttcta
     2101 cctgcaataa gtcgtttcca atctcacgtt caggtttgaa tccaacgaat ggataacgtc
     2161 tagaagaagg ttggatatcg tctaaagtaa ttttttcaag cattttttta cgtgaagttg
     2221 cttgttttga ttttgatgca ttcgcactaa agcgggcaat aaattcttgg agttctttga
     2281 tttgttcttc ttttttggca tttgcgtttg cttgaagttt agccgctagt tgactagatt
     2341 ccaaccaaaa atcataattt cctacataaa gtttgatttt accaaaatct agatccgcca
     2401 tatgtgtaca tactttgttc aaaaagtgac ggtcatggga tacaacgatc acagtatttt
     2461 caaaattgat caaaaattct tccagccaat tgatcgattg gatatccaga ccgtttgttg
     2521 gttcgtctag caaaagaaca tcaggtttac caaatagtgt ttgtgctaat aacactttta
     2581 ccttttgtcc accagttaat tcactcattt gctgatggtg caattcttca gggatgttca
     2641 gaccttgcag taaaacagct gcttctggtt cagcttccca tccattaagc tcagcaaatt
     2701 ctccttctaa ttcagctgct cgaataccat cttcgtcagt gaaatcctct ttcatgtaaa
     2761 tcgcatcttt ttctttcagc acttcataca gacgcttgtg tcccatgatg actgtttcaa
     2821 gtacagtata gtcttcatag tcgtaatggt tctgtttcaa tacggccatc cgttcatctg
     2881 gtcccatagt aacggaacct gtggaaggct gcagatcccc tgataacact ttaaggaacg
     2941 ttgatttacc tgcaccattt gcaccaatca agccatagca gtttccgggg gtaaatttaa
     3001 tattcacatc atcaaatagt ttgcggtctg aaaagtgcaa actcacatca tttacagtaa
     3061 tcaatttttt tcctcacttt tatcattatt agttttgaca agtcttatag aattatatag
     3121 gggaaaggta aaagattcaa ggcaatcgaa cattctttgt gtttcacgtg aaacagaatg
     3181 aaaaaaataa aaataaaagc ggagcgaaaa tagctactat aggatttatt agtttgcaac
     3241 agcataattt ttgacgttat ttataagagg gagtactata aaactgtaaa agaaatgaaa
     3301 gaaggattga aaatggatga aaaaagaacc atcgactggg gctcattgat tttgggaatt
     3361 ttatttgtac ttgtctcatt gttatcattt caagaccctg tcggaaattt agttgcaatt
     3421 gtagtagtct ttgcgatttt tgcctttatt aaagggatat ttgaactgtt tgtacggaac
     3481 agattaaaag agctaacagg atataaagga aagatgccct taataatcgg gatcatcgat
     3541 attctagttg gcgtcttttt cttattcaat attggtgcag gagtcgcagc attgcctttt
     3601 gtttttgcag tatggtttat tgctgattca gttttagcgt tatttactgc tgacttggca
     3661 agaggagtaa gtgaaggata ttattggttt acgatcatcg tcaatatttt aggaatttta
     3721 ttaggaattt tcttattgtt taatccaatt tcttctgcgt tgacattaag ctttttagtt
     3781 ggattctatt ttatgttatt tggtatcaca catatcgtct atgcatttag ataagtaaaa
     3841 tcaaacaaat aaaaaagccg gcttccgatt acagttttac tgttgtaatt ggaagccggc
     3901 ttttttatca tggacaaata tgcacgaggt gatttttagg tgtcgagaca aatttatgca
     3961 aaaatgtgag aagctatata cacaaatttt atcttaatac tcgaagcata gcgcagttgt
     4021 cacaacctct tttcatataa ttcattcaat actggcaaat catggacttc atatttttct
     4081 ttgaatgctt ggatttcttc ttcagaatat tttcttggat ctaaatgcag atcttctact
     4141 ggcaatgggc gcttgttatt gatcttcaca tcgattacta caggacgatc tgattttgca
     4201 gctgcatcaa aggcaaatcg aagttgcgag tattctgtga ctgtaaagct atttgccccg
     4261 agcgcttctc caactttacc gaaatctgca tcttctaaaa atacaccaaa tttcttttgt
     4321 tccgtatctt cttgttctgc ttcgatgaaa ccaaaagaat cattcgaaaa gacaacatta
     4381 ataattggaa gctgatattt gacttgtgta atgatatctt gcatgaccat agcaaacccg
     4441 ccatcaccac ttaaggtaaa tacttgtttt tctgggtagc ttagttgtgc ggcaattcct
     4501 cctggcacac cattacccat agttgcgaac catccagatg tcgtatgttt ttgtttaccg
     4561 ttcatattca ataaacgaat agaatggata gtcgtgttac cgacatctgt gacgaaaatc
     4621 gcatcgtctt cagcaatacg attgatttct ttatagacag cctctggacg aatcggttct
     4681 tcctctcgat cttcaaaaga tttcaaccaa tttacccatt tttctttatt ttcttgattt
     4741 gctaggtacc agctatcagc agggcgagca tcccctaact cgacaagtct ttgcaaagct
     4801 gtgtttgcat caccgaggat cgacaaagat gtttcatggc gtcgtccaaa ttttgacgca
     4861 tcgatatcga tttggataaa ttcagcagtt ggattaaaaa atgcacgtcc aaatgggaag
     4921 tcgctaccga caaataagat cagatcggct tctgccaatg cttcatttcc gggtttagta
     4981 gcgacacgtc cagcaaaacc aagaaagttt tcataatcat caggaataat tccttttgct
     5041 agaacggaag cgacaacagg aactgaaaaa tgttctgaga attctttgat ttgcggaaat
     5101 ccgtttcgcg ttccttgacc aacataaaga actggttttt ttgctttttc aagataaggc
     5161 aaagcagcta gcaaatcttt ttcttctggt aatatcacac ttgttttgtg agtgtgagcg
     5221 gtggagaaag gttgttcgtc aatttcttca aaaccaagat caacagggat agtcacgata
     5281 gccacgcctt tatgctcata ggcggcttta atggcttcat ctactacatg cggaagactt
     5341 tgtggtgtca tgactgtacg attgtatacg ctaacatctg cgaaaatagg attttcattt
     5401 agttcctgga aagagttata gttcatggat gtagaagcaa cttgtcctaa gaacgctact
     5461 agaggcacat ggtccatctg tgcatcatac aaaccattga tcaaatgtgt cgcaccaggt
     5521 cctgcagagc cgaagacagc gccgactttc cccgttaatt ttgcatctgc agcggctgcg
     5581 agtgcgccga cttcttcatg acgaacttga atgtagttga tttcggtttt ttcttgatat
     5641 agcgcatcca taattgaatt gaaagatcct ccgggaatgc cgtaaatatg gtcgataccc
     5701 cagctttcaa gaacttttac cattgcgatt cctgcattga tttttgacat ggtgtttcac
     5761 tcctttagca atgatgatac ataaattttt tgtatctaga gtatagaact ttttatcgaa
     5821 aatcccaact aattcgtctt ttcacaaaaa tgtagtcatg tttttgggaa tatgctagag
     5881 tagtacatag atagattcaa gagaggggaa tggcatggtt gaatggctat tgagacttga
     5941 accttggcag caagcacttg ctgggacagc ttttacttat tttatgacag ctcttggtgc
     6001 cgggttggtt tttttcttta aagaaatcaa aaaagaagtg ctaaacttga tgttaggatt
     6061 tgcttctggt gtcatgattg ctgcaagttt ttggtctttg ttagatccgg ctattacaaa
     6121 agcagaagaa aatggagata ttgcttggct agttgtaagt attgggtttg gtttaggcgg
     6181 tgtgttttta tatatggcag ataaaacact gccccatatg cattttggac cacaacatga
     6241 agcagaagga ttgcctaccc atttaaaacg gacgatcttg cttgtttttt ctattacatt
     6301 gcataacatt cctgaaggtt tagccgtggg ggtagcattc ggtgcagcag caactgcgga
     6361 taatccgaca gccgctatat tagccgctgt ttcggtggcg ctaggtattg gtatccaaaa
     6421 cttcccagaa ggagcagctg tatcaatccc gttgcgacaa gaaggtctga gtcgtaaaaa
     6481 ggcctttgtt tacggacaag cttctggaat cgttgaacca attgctggag tgattggagc
     6541 gctactagta acgaaagtgg agctgctgct tccttatgca cttgcttttg ccgcaggagc
     6601 aatgatttat gtcgtggtgg aagaattgat ccctgaagca cagcagacat tgtccagcaa
     6661 acgtcatttt gcagtttttg gcgtcatgag tggatttatc attatgatga ttttggatgt
     6721 ggcattagga taaataaaaa aaatatatat gatatatcaa atagggccgc gaacttatat
     6781 cgcgacccta tttttttagt aagttatatc ataatgaagt gaatggtatc atacattaat
     6841 aatagttttt tctatgtgta tttcctttga tccagcgaac aagccagatg ataagtgcga
     6901 ttgggattag aactttgacc gcaaaccata aaagcccgct taggacactg acaaaaagac
     6961 tgatgacgaa gctagctgca attagcacta aaagaaccat gaagatattt aagaacatat
     7021 ttttttgacg catacaaaac cctcctcttg ttcattcttt actatagaaa aagtataaca
     7081 gggattgttt aaaaacacct ccaactgaag actgattttt acttcatcta cttaaatgat
     7141 caaacaattg ctttttctct ggtttatcat ctaaaataga gcggaaaagg aggagaatat
     7201 catgcctaaa aatcgtcttg aagcatttac agatgctgta atcgcgatta tcatgactat
     7261 tttagtttta gaacttcatg aacctaacgg agacacgtgg gaggcgctcg ctgcattagg
     7321 ttaccgattt tttatctata ttgtcagttt tatcatttta gcgatttatt ggaacaatca
     7381 tcatcattta tttcaagttg tacataagat aaatggacgg gtcttatggg cgaacagttt
     7441 ctttgttttt gcgctttcgc tttttccgtt tgcaacttca tggatcagtg agtacatcaa
     7501 tagcctggct ccagaaatga cttatggttt tgttaccttg accgctaata ttgcgtatta
     7561 tatcttggca aaagaacttg tcaaagcaaa taaaggccac gagaaagcat tgaaggcgat
     7621 gttgaataat tacaagaaat cgtatctatc gattggtttg aatgtgttag gaattgtctt
     7681 agggtattta gtagcgccag tagccgtgct gatcgcaaac atcgtgattc tagctgcttg
     7741 gatcatccca agtcgtacca tcgaatcaca atatcggtaa aatgatccgt gtttaaaatt
     7801 taagagaaaa atatagaaaa aataaataaa cgatcaaata tccgagtgat actagtattc
     7861 aaagagggat gcagatggat caaaaaagaa ttcatgtagg actgattact agttttgtca
     7921 cgatgcttat ttatttggat caaggtcatt tgacttggac agtagttggt gtcgtaggat
     7981 ttttgacatt attcacagca ataaaaaaat aaacatcttt atgtaatctg atgttctatc
     8041 tatttaagaa aagcatgttt acagcagtat gattctgttg taaacgtgtt tttatttgtg
     8101 gaaaatttaa tttttagaaa agtgtatcga aaaatagata gttagttttt ccacagtgga
     8161 taacttcgta agagataaaa ataaaaaatt agtcaaagaa gggcgtatag atttcttttg
     8221 ataggagata tacaacagaa atgaataaat attaacgtgt tttcaaacag tataaaacgg
     8281 tgtg
//
LOCUS       DAHHZJ010000125         8143 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.126, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000125 DAHHZJ010000000
VERSION     DAHHZJ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.126"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            379..1917
                     /locus_tag="NBL02_002181"
     CDS             379..1917
                     /locus_tag="NBL02_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HCD2572858.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            1914..2879
                     /locus_tag="NBL02_002182"
     CDS             1914..2879
                     /locus_tag="NBL02_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2572859.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            2866..4188
                     /locus_tag="NBL02_002183"
     CDS             2866..4188
                     /locus_tag="NBL02_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCD2572860.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      4251..4355
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4429..5172
                     /locus_tag="NBL02_002184"
     CDS             4429..5172
                     /locus_tag="NBL02_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2572861.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            5187..6179
                     /locus_tag="NBL02_002185"
     CDS             5187..6179
                     /locus_tag="NBL02_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCD2572862.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            6191..6928
                     /locus_tag="NBL02_002186"
     CDS             6191..6928
                     /locus_tag="NBL02_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2572863.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            6918..7640
                     /locus_tag="NBL02_002187"
     CDS             6918..7640
                     /locus_tag="NBL02_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HCD2572864.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(7724..>8143)
                     /locus_tag="NBL02_002188"
     CDS             complement(7724..>8143)
                     /locus_tag="NBL02_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HCD2572865.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         2603 a         1411 c         1769 g         2360 t
ORIGIN      
        1 caaccatttt ttacattttt ggcaaaaaag tgtcatgata cagtataata ttcgttgaac
       61 aggccaataa aggtgttctc ccccacgaga tttctacttt attggctgtt tctttttatt
      121 aaaataaaag ttgtgtgtgt aaaaaagata tagctgattc caagtgacaa tttcgtatag
      181 atttttatgg tattcgtagt gaaaaaacga gaaccaaaaa gttgatccga ctttgttgct
      241 tggattcttt ttttgtcagc atatttagag gtattttttt cttttttttg ttcgtgctgt
      301 aaggttaagg caagacaagt tagttgggaa aaaggaaaga aatgtttata cttaaagaat
      361 aacagaaagg aaggtcgcat gaataatttt atacaaacat taagtgaaag aaaaggcgag
      421 ttgctggaag ctgcatttca acatctgtcc atttctctca tttctttgct gattgcagca
      481 ctgattgcta ttcctttagc gatttgggca gccaatcata aaaaaatggc agaagtctta
      541 ttgcagatca cgggtgtgct ccaaacgatt ccttcacttg ccttattagg tttacttatc
      601 ccatttgtag ggatcggaac tgttccagca ttgattgcac ttgttattta cgctttgctt
      661 ccgattttcc aaaacacgta tattggtcta gctgaaattg acccgtctat tgaagaagca
      721 gcggtagcat ttggtatgtc cagaatgaga cgcttgctga aagtagagct tcccatcgca
      781 ttacctgtca ttatctcagg tatccgaact gccttagttt taatcatcgg tacggctact
      841 ttggcagcat tgatcggagc aggtggatta gggacattta ttttgttagg tattgaccga
      901 aatacacctg ttttgacatt gattggtgcg atcagttctg ccttgcttgc aattatattc
      961 agcggactga ttcgctggtt gcagcataag aaacctcgat attccatcat tactttagca
     1021 gcagtggtgt gtttatttgg cggcgtttct ttatatcagt ctgccttgtt taaaaatgaa
     1081 accatcacga ttgctggaaa attaggagcg gaacctgaaa tcctgatcca aatgtataaa
     1141 gaattgattg aagacaacac aaaaacagaa gttgaattaa aaccgaattt tggcaagacg
     1201 agttttttgt tcaatgcact ggatcaaggt caaatcgata tctatcctga gttcacaggt
     1261 actgtcctag aaagcttggt gaaggtacct gaagcaatga aaagtgctga tttgacaaaa
     1321 gaaaaaacat atgacgaagc agaagaatta ttgaataagc aattcgatat gacattatta
     1381 aaaccaatgg cttaccaaaa tacgtatgcc ttggcagtca agagatcttt tgcgaacgaa
     1441 catcaactta aaaagatttc cgatttggca gctatctctg atcagatcaa agcgggattc
     1501 acactggaat ttattgatcg ttcagatgga tataaaggaa tccaagaagc atatggcttg
     1561 aatttttcaa gtgtacaaag catggagcca agtttgcgtt accaagcaat caataatgga
     1621 gatgtcaata ttgtggatgc ctattccaca gatagccagc tcaaggagta cgatctagtg
     1681 actctagaag atgatcgagg gttattccca gcttatcaag gcgcaccgct gatgaaaaaa
     1741 gattttgcta aagaacatcc tgaaatcgtt aaagcactga ataaattaag cggaaaaata
     1801 acagaggatc aaatgattga tatgaattac caagtcaatg tcgaaaagaa aaagccgaca
     1861 gaagtagcac gaatcttcct gaaaaatgaa ggattagtaa aggaggaaga tcagtgacta
     1921 aattaatcga gttcaaacat gtgcaaaaaa agtatgatgg aaagtatgtc atcgatgacc
     1981 taaatctggc aatcaaaaaa ggagaaatct ttgttttggt tggtccttcc ggtagtggaa
     2041 aaacaactac tttgaaaatg atcaatggat tatctaaacc atcagcagga gacatctact
     2101 tcaaaggaaa atcgttaaat gaatataatc tgcagaaaat gcggtggaat atgggatatg
     2161 tgctgcagca aatcgctttg ttcccgacca tgacagtcaa gcaaaatatt gaagtcatcc
     2221 ctgaaatgtt aggttgggaa aagcagaaaa gagcagaccg tgtagatgaa cttcttcaaa
     2281 aagttggctt atctcctgat atttatcgag atcgtatgcc tagagagttg tctggaggag
     2341 aacagcaacg gatcgggatc atccgagcta ttgctgcaag cccagatgtg attttgatgg
     2401 acgagccatt tagcgcatta gatccgattt caagaaactc attacaagaa ttggttttgt
     2461 ccttacacga agaattgggc acgaccatcg ttttcgttac gcacaatatg gaagaagcaa
     2521 tcaagctagg tgatcgtatt gcatttatga aggatggaga aattatccaa tgtgacacac
     2581 cagagcagtt attgatgaat cctaaaaacg actacgttcg gcatttcttt gatgaaccga
     2641 aacagacgaa agagtggcga gtagaagatt tagtcgtcaa tggatatttc ttaaatgaaa
     2701 taccagaaaa tgctcgtcaa caagtttgct ttgatacgcc tatgaaagaa gtatattcgc
     2761 ttctttcggt gtatccaagc attgtgatag tagaaaagca aagaagtatt gggtcgctga
     2821 caagtaaaga aatctttgct tttttaagtc gagaggagga aggaaatgaa aacatatgat
     2881 gcaatcatca tcggttcagg tgtgagcggc ttaagtgctg cttatggatt gaaagaagca
     2941 ggtaaaacag tactagtggt cgaagaagat ctatggggcg ggacttgtcc taaccgtgga
     3001 tgtgatccta aaaaagtctt gttaagtgca gtagaggcac gtaatcgggt aaaacaattg
     3061 agtggaaaag gttttaacga gatcccaact gccaactggg aagaacttca aaaattcaaa
     3121 cgaacattta cggatccagt cccagaaagc cgaaaaaaac aattagcaga agctgaaatc
     3181 gatcatcttt caggaaccgc tcgttttttg gatgattcat ctattgaagt caacgaagaa
     3241 gtcttccatg cggattactt agttttggca accgggcaaa gacctacgat cttaccagtg
     3301 gagggaaagg aatacttgaa aactagtgca gactttttat cattgcctgt actgcctaaa
     3361 gaaatcattt ttatcggtgg tggttatatt gcttttgagc tagctacgat tgccaatgct
     3421 gcagggagca aagtaaccat tgtccatcat aatcaacggc cattaaaaga atttgaggcg
     3481 tctttggtag aggaagccgt ccatcaaatg gaagcatctg gcattcagtt tgcttttggt
     3541 gttgaaaccc aaaagatcat ttcagaaggt actcgctatc gtttagtcgg aaaagagacg
     3601 gaattagtag cagatatgat tttttgtgcg actgggagac agccgaatac agaatcttta
     3661 gcacttgagc aagcgaatat cgtctttgat aaacacggaa ttgcagtcaa tgattatctg
     3721 caaacgagca atccaaaaat ttttgcgtgt ggagatattg tttcaaggaa aaccccaaaa
     3781 ttgacaccag tcgccacttt tgaaggaaat tatgtagcta agcggataac ggatgcaaca
     3841 agcgaaccta tcaaatatcc gattatacct acgatcgtct acgccagtcc taaacttgct
     3901 gaagtaggag ttacaaaaag tcacgcctct tcgtcagatc aagtggtaga gatggatctt
     3961 accagttggt tcacctatca tcgtgtcaat gaaccagttg ctaaggctga attgaccttt
     4021 gaccagcaaa attatttgat tggtgctgca gtgattagcg agcaagcaga cgaattgatc
     4081 gatgatttga cactcgtcat caatcaaaaa ttgactaaaa aagagttgga tagctatatc
     4141 atggggtatc cgacattggc aagtgatctc tcctatttat tgaaataatc agcatgataa
     4201 ctaaagcgct tgttgctttt tccttcttgt gctacacttt aaaaaatgaa tacctgtttc
     4261 tggggatgtc gtagacgaca tgagagtaag ttgtgaactt agacccattg aacctgtcag
     4321 ttaatactgg tgaagggaga aacatagcag acgcttgaag cgggttacta tgtcgtgccc
     4381 atttcaagcg ttttttgtta ttcatattag aagaaaggag gtaacaaaat gaagaaaaat
     4441 ctttatccag cagtcagtat ggcattcatt ttaattattt ggcagattgt tgtcatagtt
     4501 tggaaaatac ctgcctttat tttacccgga cctgctgctg tattttctgc attagcggca
     4561 gatgcacaag tattactttc ccattctgtc acgacattaa atgaagcagt gcttggtttg
     4621 gtaatagcta gcgttctttc ttttattact gccttggcaa tggattattg gaagttttta
     4681 gaaatgtctt tttatccgtt actagtgatt tcacaaatgt tgccaatcat ggtgttaggg
     4741 ccattactta ccctatggtt tggtttcggt atgcttccaa aagtgatcct tgttgtcttg
     4801 ataagctatt ttccgatcgt agtttctttt tcggatacat tgaggaaagt atcaaaagag
     4861 caaatcatct ttttgaaaac gatgggggca gatacaggga agatttatcg aatatataaa
     4921 ataccagcgg gtttatccgg cttcttttcc ggtttaaaag tggctgctac ttattgtgtc
     4981 agcggagcag tcgttggaga atggctaagc gctcaagcag gcttagggta ttacatgata
     5041 cgcgtaaaaa acagctacca gatcgataaa gtttttgctg cgatcatttg tgtcattcta
     5101 ttgagtttat tgctgaatgg cgcttgttca ttattgaaaa aagggtatta tcgtatgctt
     5161 tatatttttt aagagaaggg gatcaaatga aaaaattatg gcttttatta ccgctactgt
     5221 tgcttttatc cgcttgtggc actgcaaaag aaacaagtag caaacaagaa ataaaggatt
     5281 tgaaaaaagt gacacttgtt ttggattatg taccgaatac taatcatact ggcatctacc
     5341 tagcaaaaga aaaaggctat tataaagagg ctggtttaaa tgtccaaatc atcgaaccag
     5401 gagataattc gacaagtatt ggtctagtag gtgcggacaa agcacaattt ggtgtaagtt
     5461 atcaagaaga tgtcacttat gctcacgcag atggacagaa tataccagtc aaagcaattg
     5521 ctacagtgat caaacataat acttctggat tcgccaccct atcagacagt aacatccatt
     5581 caccaaaaga ttttgaagga aaaacatatg caggttggca aagtccaagt gaagaagctg
     5641 tcttaaaagc tgtgatggaa aaagacggcg gggacttttc aaaactgaca atggtaggaa
     5701 gcaatggaga agggccagaa agcttaggaa aatcctcaga tatccaatgg tactttgaag
     5761 gttgggatat gatcaaagca aaagaagcgg gtattgaact aaactatatc ccattgaaag
     5821 aattagatga acggttagat tactatactc cggtaattat tacgaatgat caactgatca
     5881 aaagtgatcc agaattggta cagtctttta tggatgccac gaaaaaaggc tatcaggaag
     5941 ctattaagga tccaaatgac agtgctaaac tgttgcaaaa atatgcaaaa gaaaatgatc
     6001 ggacgttttt agaagaatca caagcattct tgtcaaaaaa ttatacggat gatcctgaga
     6061 attggggatt gatggaagaa aaagtttggc gcaattatac agcttttatg caggaaaacg
     6121 gattgatcaa gcaagatgta ccttcgcaag aactattcac taatcagttt ataaaatagg
     6181 agtgatagat atgtcaatcc aaatcagcca cttgaatgta gtcattgaaa acctgaaaat
     6241 attagaagac atccatttca ctatccctaa agataccttt acatcgattg tcgcgccaag
     6301 cggagccggt aagtcaacat tgctgaaaac tttaacagga gtccagccta tgacttctgg
     6361 aacgatcaaa gtagacgatc aaaaagtaac tggtctccat acagcattta gttatatgcc
     6421 gcaagaagat atgttacttc cttggctgag cgtctatcaa aacgttacgt tatatcaaaa
     6481 aatcaatcat ctaacaatca atgaagaaca agtaagggat tatctggata tctttgggtt
     6541 aaacggctat gaacattttt tgccggaaca attatctggc gggatgaagc agcgaacggc
     6601 cttgttacga acaatcatga atccttcttc ttacttgctt cttgatgaac catttggagc
     6661 gttagatgct atgaccagag ggcagatgca agactggatg ttgaagctgc ctaaaaaagc
     6721 aaagcggact actttacttg tgacgcatga tatcgaagaa gcaatttatt tatcagacag
     6781 gattcttgtg ctttccgccc gtccggcaca tgtgattgct gagattcaag tgcctgaaaa
     6841 acgacggtca cgtgaatggc tactgcagca atcagagttg aagcaaacga tttatcagtt
     6901 attggcaggt gaattagatg ttaagtgatg ctcattgtca tttagatggg agtcagagtt
     6961 tagcactcct tcagcaggaa cacacgattt tgacgatcat caattgtgat tcgccagaag
     7021 aatggaagga aaacagacag cttgcagcaa gtaagactca agccttaagt tacggtatcc
     7081 atccttggaa agcagatagc tatacgtttg aacaagttga gccttttttg aaaaaagcaa
     7141 ggatcatcgg tgaaataggg ttagataata tttggacgaa tgtaccgatg actacacaaa
     7201 aaaaagtatt tgagcgacag cttgcttttg cagccataaa tgagaaacct gttgtactgc
     7261 acacaaaagg atgcgaaaaa gagatcttaa attatatcca agcttaccct aatcactatt
     7321 tgatccattg gtattcttct ttggactatc aacaagacta tattgattta ggctgttatt
     7381 tcagcattgg tcttgatttg aaaaagaatc cagcagtctg gcaactagct aaagcagtcc
     7441 caatcgacca cttacttatt gaaacggatg gactacatgc agcagagtgg gctttagggg
     7501 ggattttttc agaaaaagat tatgtctcac tgcttgaaga acagttaaag acaatcgctg
     7561 agatcaaaga gctatcagtt gaagaagtta aaaaaaatac ttatcaaaac ttggaacgtt
     7621 tcattagatt aggtaaataa aaaagagatg tgacacaact agtgtcgcac ctctttttgg
     7681 ctttctttgt ataaaagtaa actgtttgat taataaatga atcttagtac caaccgtttg
     7741 cttcccagaa tgctttcgcc gcttcccaag aaccataacg tgaagtaaca tattgttctg
     7801 ctactctttc ttggtttgca gcagagtagt caccatttaa gtaagaagaa tctaattggt
     7861 aacgtccgat gtaacgtccg ttcgttgctg tgtaagaacc gcttgattct ttttgtgcga
     7921 tccattcttt tgcagagctt gtgcttgctg gagtagcagc agtttctgtt acagcaggag
     7981 caggttgtgt ttcagcaaca ggcgctgctt gtttttccgt ttgaaccgtt tcttgtacaa
     8041 caggttgttc agcttggact ggtgcttctt gaacggctgc ttgaggttca gcagctgctt
     8101 gtgcagagcc ttctgttggg attgttaatt gttgtcctga gta
//
LOCUS       DAHHZJ010000126         8108 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.127, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000126 DAHHZJ010000000
VERSION     DAHHZJ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.127"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            131..964
                     /gene="msrA"
                     /locus_tag="NBL02_002189"
     CDS             131..964
                     /gene="msrA"
                     /locus_tag="NBL02_002189"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCD2572866.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            1077..2033
                     /locus_tag="NBL02_002190"
     CDS             1077..2033
                     /locus_tag="NBL02_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCD2572867.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            2322..3020
                     /locus_tag="NBL02_002191"
     CDS             2322..3020
                     /locus_tag="NBL02_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCD2572868.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            3170..4486
                     /locus_tag="NBL02_002192"
     CDS             3170..4486
                     /locus_tag="NBL02_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCD2572869.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            4503..5918
                     /locus_tag="NBL02_002193"
     CDS             4503..5918
                     /locus_tag="NBL02_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCD2572870.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            6383..6703
                     /locus_tag="NBL02_002194"
     CDS             6383..6703
                     /locus_tag="NBL02_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCD2572871.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            6729..7046
                     /locus_tag="NBL02_002195"
     CDS             6729..7046
                     /locus_tag="NBL02_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCD2572872.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            7075..>8108
                     /locus_tag="NBL02_002196"
     CDS             7075..>8108
                     /locus_tag="NBL02_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCD2572873.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLV"
BASE COUNT         2666 a         1320 c         1721 g         2401 t
ORIGIN      
        1 tgtcgataag gattctctct tttttatacg aaaaatctgt agggggatga agtaatcgct
       61 tatttcctca ctttatgata aattacagct aaagtcacta gcttgtcttc ttcggaaagg
      121 aggagaatct atggatgagg aacaaatatt cattcaaaca ctttataatc tgattttgaa
      181 tccgaataca cgagattggg aacgaaaagt attgattcag acaaagaatg acataagaga
      241 aaatatatcc gttaaagaac agttatcaaa actagaagca acactacgtc cattagctat
      301 ccgaatgaat ctgacgcctg atgtaatgga cttttattta ttgattacgg aaggatttga
      361 taaggagcaa aaatatgact tttccaagca tgccatgcaa gatgctgact atcaagaacg
      421 tgcagtattt gcagggggat gtttctggtg tatggtcgag ccgtttgaat cgaaaaaagg
      481 gatcctatct gttttgtcag gatacacggg cggacatgtt gaaaaaccaa attatgatca
      541 agtaagtggt ggttacacag gtcatgtgga agctgtggag atcatttatg acacaagaga
      601 aataagttat agcgagttgc tgacaatcta ttggcaaatt actgatccca cagatacatt
      661 tggacaattt caagatagag ggaagcaata tcgtccagtg attttttatc aagtcgaacg
      721 acaaaaagaa ttagcggaac aatcaaaaca gaaactagac agttcaggaa ctttccacca
      781 accaatcgtc acaaaaatcg aaccagccgg tacgttttgg ccagcagaaa actatcatca
      841 gcaattttac aaaaaacaac cgaaacgtta taaaaaaatc cagcaagcac gtaatcaatt
      901 tctgatctat caacgagtaa aaaacaaatg gcaaaaaaat atacgaaaaa atcattttga
      961 ttaactcctt ttctttaaaa tgtagtaaac taaaaataga aaaaagaaag cgttttaaaa
     1021 agatttgatg aggaggagaa aaattgtata ttgcagatag tgcacgttat gaaaaaatgg
     1081 aatatcgtaa atccggtcat tcaggtttga aattaccgat tttatctctc ggattatggc
     1141 aaaattttgg tgattatgat cctatccata atcaaagaga gattttaaga ggcgcattcg
     1201 acatgggaat tacccatttc gaccttgcga ataattatgg aggccctgct ggagctgcag
     1261 agaaaaactt tgggcgtatt tttagagaag attttcaagc ctaccgtgat gaactgatta
     1321 tttctagcaa agcaggttat catatgtggg aagggccata tggggaatgg ggctctcgta
     1381 aatccatcat ttcaagttgt gaccaaagtc tccaaagaat ggggctagat tatgttgaca
     1441 tcttctatca tcatcgccca gaccctgata cacctttgga agaaacagca gaagctttga
     1501 tgcaacttgt aagacaagga aaagcattat atattggtat atccaactat aatggagaag
     1561 atacaaaaaa aatgactgaa attttaaaaa gaaaggaagc accgttcatt attcatcaga
     1621 tgcgctacaa tatgttttct cgtgcactac tagaagacga tctatctcca gtattagaag
     1681 aagaagggct aggggcaatc acgttcagtc cgcttgctca agggttgctg acaaatcgtt
     1741 atctacatgg tattcctgaa gattctagag cacatcgtaa ggaaatacca ttcctttcag
     1801 aagaacaagt tggatcaact ctggaaaaaa tcaaagcact gcaaacaatc gccgtatcac
     1861 gaggacaatc attagcgcaa atggcccttg catggaattt gcgacaaaaa tctgttacaa
     1921 gtgtactggt cggagcaagt cggttaagtc agttacaaga aagtgttcga atgatggata
     1981 atttagattt ctcaccagaa gaagaattga gaatcgacca aattctagaa tagaaacgat
     2041 gtaatcaaac tttttatcca tatattaaaa aaaacaaaag atgacatcaa gaatcgaatg
     2101 aagatcgtct gttattgaac atcatatttt ttgtcgttca aaataaaaat gatcgaaggc
     2161 taggagtgaa ttttatccta gtctttttta tggtaaatat cttcttcgtt tgtatgtcta
     2221 tgaacggatt aatcaatttt gtatacaaaa aatcatcaga tgattcaaac gtgttataat
     2281 aattacgaaa aaatacatca gatgaaaagg aggtatgtgg tttggaagaa aataaaaccc
     2341 tagtagaagt aactactgac aagatcctta gatatttaaa ggaaatgcag ttgcaagtag
     2401 gagataaact gccgaatgaa tatgaattgt ccgattattt agaggtgggg cgaagcacgg
     2461 tgcgagaagc tgtgcgggct ctagcttcca ggaatatcct tgaagttcgg caaggagcgg
     2521 ggacatttgt cagtgataag cgagggattg ccaatgaccc actcggtttt tctttgattg
     2581 aagatcagga tcggatgata cgagacttat ttgaactgag atatttattg gagcctaaga
     2641 tggcaggttt ggcagcggaa tatgcaacga agaatcaaat cgtccaaatg aaaaaattaa
     2701 cgcaaaagat tgaaacttct tttcgagaag gcacacaaga acatgttcaa ttagacatcg
     2761 attttcatac gatgattgca gaagctagcg gaaacgtagc cttgctgcat attatcccaa
     2821 tcattaatga atcaattgct ttgtttaata aaaattatga tatccagcat ctgaaaatcg
     2881 agacgatcga tattcataca gagatcgttc aggccatcga aaggcggaat gcacttgctg
     2941 cgtttgatgc aatgactgtc catatggcaa taaatcggag agaattacag aaaataatag
     3001 gagaaaaaga aactaattga cagttcatct gatgtatttt atgataaata catcagatga
     3061 atttttgttt tatatttggt aaaaaagagg agtaaatagt ttctgttcat cgaaatgtaa
     3121 gcgctgacta atagacaaaa agagatgacg tcaaggagaa gaagcaggaa tgaggggatc
     3181 tggaatgagt tttatggata aattagccaa tgttttaggg aacgtggcaa cgagaatcaa
     3241 taatttaagg tatatcatgg tgataaaaaa cgcatttgct gcattgattc cggtaattat
     3301 tacgggtgct tttggcacat tgttttcagc aatggtcttt gactcggaaa atggcttagc
     3361 acagataagt ttcttgcgtt ttttagaaga attaaagcct atcgcatctg ccatcagcta
     3421 tgtcacacta agtttcttga ccatctatgc tgtttttctg atagggatcg aattagccaa
     3481 gctgaataaa gtggatggtg tattccctgg tatcatcgca gtgatgtctt atttatctgt
     3541 taatccaact gtttatgaat tagtgaatga aaatcagact gttatagtag aaaacgtatt
     3601 ggctaaacaa tatacagata cgaaagggtt atttttggga atgttggtct ccatcctttc
     3661 tattgaactt tattgctggt tgaggaaaca gaagaaacta cagctgagaa tgcctgatac
     3721 cgtgccaacg aatgtttctg ctagtttttc tgctttattc ccgacgattc tgactgtatc
     3781 gggtatcgcg gctgcgggat ttatcatcaa agaacttaca ggaatgtacc tttatgatat
     3841 catttacaat gtcgtacaac gcccattaga aggtgtcgta caagggttac cgggtatttt
     3901 gctattgatg tttattgcgc aagtcttttg ggtgattggt atccatggaa atcaaatggt
     3961 gaagccgata cgggaaccgt tgcttttagc ggcaattgct gtaaatacgg aagcttttga
     4021 agctggaaaa gaagtaccga acattattac tatgccattt tgggatatgt atatgagtat
     4081 gggcgggtct ggagtcacga tcggtttact gattgcagta ttgttagttg gaaaacggga
     4141 agatatgaaa caaatcacca agttatccct tgctcctggg atcttcaaca tcaatgaacc
     4201 agttattttt ggtatgccga tcatgctgaa tccaatttta gctattccgt ttatcttaac
     4261 gcctcttatc actggaacga ttggttattt tgcaaccgct ttaggatttg cggcgaaagc
     4321 agttgtaatg gtcccttggc caatgccgcc gatcgtcaat gcttatctgg caacagcagg
     4381 aaatatcggt gcagttatta cgcaacttgt ttgtattatt gtttctattt tgatctatct
     4441 gccatttgta aaagtagcaa atcgtgtgga aaatgatggg atctaaaaaa caggaggaaa
     4501 tcatgaaaat tactattcca aaaaatttta ttcttggtgc tgcatcttct gcttggcaaa
     4561 cggaaggttg gaaaggaaaa aaagaaggac aggattcgtt tatagatagc tggtataaaa
     4621 atgaacattt tgtttggcat aatgggtatg gcccagccgt agcaactaat tttatggaac
     4681 aataccagca ggatgtggat ctgatgaaat ccatccatct gacacattat cggacttcta
     4741 tcaattgggc tcgttttttt actgattatg agaactgtat agtagatgaa gaatatgctc
     4801 agcatataag tgaaatgatc gactctttga tcgaagcaga cgtagaaccg atgatttgtt
     4861 tggaacatta tgaagtccca gcttttcttt tggaaaaata tgatggctgg tcttcaagaa
     4921 aagtagttga tttttatgct aagtatgctc aaattgcctt tgatcgttac ggtgataggg
     4981 taaaaaactg gtttacattc aacgaaccaa tcgttcccca aacgcgttgc tatttggatg
     5041 ctgttcgctg gccgcatgaa caaaacacaa aaaagtggat gatgtggaat tatcataaag
     5101 ttttagcaac ggctaaagca gtagagattt accgcagcgg aaattatggt ggaaggattg
     5161 gctgtatttt aaatcctgaa atggtctatg ctcgatccaa ttctccagaa gaccaaaaag
     5221 ctgcttatat gtatgatttg ttttataatc gagtattttt tgatccgatg gtaaaaggat
     5281 actacacaga agagttcctg gagttgtgtc aaaaacaatc gatttatttc gatcctgcaa
     5341 cagaggattt agaaacgatt cagcatcata cagtagattt ccttggaatc aatcaatatt
     5401 atcctaaacg cgttcgcgcc cctcgttatc aatggaatga agagacacct ttccatcctg
     5461 aacgatatta tgaagtcttt gatttacctg ggaagaaaat gaataattcc aggggatggg
     5521 aaatctatcc gaaaatcatg tatgatatag ccatgtatct aaaagaaaat tatcataata
     5581 tcccttggct gataacagaa aacggtatgg gaagagaata tgaagaacag tatatggatg
     5641 agaaaggaat cgtgcaagat gattaccgta tcgaatttat cggacagcat ttgcattact
     5701 tgatcaaagc aacagaagaa ggcgcaaatt gcgaaggcta tatgttatgg gcattcactg
     5761 actgtgtgtc accaatgaat gcatttaaga atcgatatgg attagttcga atcgaattga
     5821 acgatgaacg aagcagaaca ttgaaaaaat ctgcttcgtg gtatcagact ttgattgata
     5881 agagagaatt ggatttgcct gacgaaatct ataaataaat agaagttttt tgtagatgtt
     5941 cattgtattt actggttatt tgaaataaaa gaggttgtga atccagcgtt ttgagctaat
     6001 acccagggtc agctgattga acaccgttta ttgagaggtt gtgaaaaagc tgtcctgcat
     6061 caagtaataa gaatagcaaa gtaaaaacaa catcttatgt ttttaacttg gttggactta
     6121 ttaccgcaga tgcaagcttt tgaataccgt ttatttgaaa aaagttgtga caagttttgt
     6181 gtcacaactt tttttgtgca cgttaaaaga aaaaaggtaa taaaaattaa tttgtgtgtt
     6241 tagtgtgcgc tttctttttt tagtgtgtat gcaacttggt atagacctta aatgtgatga
     6301 tatcaacttt ttaaagcgct tttaaagttg gcacgctact tgcttatata caagtgtaag
     6361 tatagaaagg aacgtgttta aaatggaaaa gaaaacaatc atgcttgtat gttcagctgg
     6421 aatgagtaca agtctcctag tcacaaaaat gcaaaaggca gcagaagcaa aaggaatgga
     6481 agcagacatc tttgctgtat ctgcttcaga tgcggataat caactagaat ctaaaaatgt
     6541 agacgtttta cttttaggac cgcaagtacg ctttatgcaa aatcaatttg ctgaaaagct
     6601 agcacctaaa ggcattcctt tagatgtaat caatatggct gactatggca tgatgaatgg
     6661 tgaaaaagta ctcgaccaag cagaaaaact aataaacaaa taataaaaat tatcggagga
     6721 tacaataaat ggaagatcaa aaaaatctag aagccatcat gggtctgatc atgtttggcg
     6781 gaaatgcaaa aagcgatgca atggaagcga ttgctgctgc gaaaaaaggt gatttcactt
     6841 tagcagatga aaaaatcaaa gatgcagagg aatctttagt ccaagcacat cactctcaaa
     6901 cagagatgct gacacaagaa gcacaaggaa atcacgttga agtcacgctt ttgacggtcc
     6961 acagtcaaga tcatctgatg acatctatag cttttacaga tttagcaaaa gagatcattg
     7021 atctgtatcg tcgattgaac gcgtaatcta cagtaaagaa aagagggaag atttatggat
     7081 aaatttgttg catttatgga gaaacatttt atacctgtag catcaaaaat tggtgcacaa
     7141 cggcatttag ttgcgattcg ggactcattt atggtgagta tgccgttaat gatcttagga
     7201 gcattagcag taatgatcaa taacttaccg attcccgggt tccaagaatt gatgaactca
     7261 atttttggtg gtgaatcatg gaaaggattc ggggctgctg cttggaacgg tacatttgcc
     7321 attctttctg tcttgattgc tttcttgctt gcttatcatt tagctaacgg ctaccgaaaa
     7381 gacggtgttt ccgcaggtgt gatttcttta ggctctttct tcgctttagg cggagcatta
     7441 ggtatgagct caaacggttt gtttattgct attattgttg ggattatttc aacagaaatc
     7501 tttgtacgtt taagcggaaa tgaaaaattg atcatcaaaa tgcctgatgg tgtgccaccg
     7561 gcagttgcaa aagcatttgc ttcattacta ccagcaatga tcactatctc tgcgtttgct
     7621 ttagttgctg caatttttgc tgcattcggt gttgatgata tcgtaggttc tttctataca
     7681 gtggtccaag aaccatttat gggacttgca aactcttatc catctgcctt actgcttgca
     7741 tttatcacac cattcttatg gttctttggt ttacacggag cgaatatggt cgatcctttg
     7801 atgcaaacaa tcaatgcgcc agccattgaa gcaaacgtta atgcaatcgc agctggacat
     7861 agcgcaccgt ttatcgtgaa caaacctttc tttgacagct ttgtcaactt aggaggaaca
     7921 ggtgcaactt taggtttact gttagctatt tatttagtag gacgtaaaaa caaaccatat
     7981 atggttgtaa caaacttgtc gattgcgcca ggtgtcttca atattaatga accaacgatg
     8041 tttgggttac ctatcgtgtt gaacccgatc atgttcattc catttatttt gacaccaatg
     8101 gtcttagt
//
LOCUS       DAHHZJ010000127         8047 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.128, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000127 DAHHZJ010000000
VERSION     DAHHZJ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.128"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            381..569
                     /locus_tag="NBL02_002197"
     CDS             381..569
                     /locus_tag="NBL02_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCD2572874.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            647..1660
                     /locus_tag="NBL02_002198"
     CDS             647..1660
                     /locus_tag="NBL02_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD2572875.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(1727..2761)
                     /locus_tag="NBL02_002199"
     CDS             complement(1727..2761)
                     /locus_tag="NBL02_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCD2572876.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            2876..5101
                     /locus_tag="NBL02_002200"
     CDS             2876..5101
                     /locus_tag="NBL02_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCD2572877.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            5119..7002
                     /locus_tag="NBL02_002201"
     CDS             5119..7002
                     /locus_tag="NBL02_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HCD2572878.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            6983..7957
                     /locus_tag="NBL02_002202"
     CDS             6983..7957
                     /locus_tag="NBL02_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HCD2572879.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         2599 a         1468 c         1703 g         2277 t
ORIGIN      
        1 ttagtctaca ttattttggc gatttgccaa taaggactgt tgagttgtgc aaaatcaaat
       61 ctgggtcaga atgaacgtca ttctgtctca gatttttcgt tatttcaatt tgattaactg
      121 attgatcaaa aagattttta ttttcatatt ctcaaatgat tcaacactaa atatacatgt
      181 ttgacgcgga aattattatt cgattttggc ggcatcaggg gggatgacga cgtagcagac
      241 ccattatttt actcaagaaa atgtccgtgc ctattattta gaatacgata gtgaacgtgc
      301 tgggagtttt gttcttttag agaaagtctc tgaagataaa ttagttgtct tagattttga
      361 ctgtaacgaa aaatggcgaa atggaagagc accagcaagt gattgaccgg atctacgaag
      421 cgacacgata tctgcctttg gatcgtttgt gcttaagtcc tcaatgtgga tttgcttcaa
      481 cagaagaaga aaatgttttg tttgaagaac aatggaaaaa atttcttttg tcaaaagcat
      541 tgcagaagaa gtttggggaa aatcattaaa tcatctagga gagcttgata ggaaataaca
      601 ctatggtata atcagaatgc ttaatactaa ctactgaggg agccttatga aaaagaaaat
      661 tcttaaattt atcggatggt tcttcggaat cattcttggg atcgcgtttg caatctttct
      721 tgccttccag ctttccccca gacctggagc attcttgatc aaccatatgt tttcaaatac
      781 tgttcaaatc actgataaaa aaacgttcga tcaagcagaa aaaaacgtgg cgaaaaaaac
      841 agatttgacg tatgagtcca agtataaaga gaatacttat gacatctact atccaaaaaa
      901 cagcaagggg cctgttcctg ttctattttg ggtacatggt ggaggattcg tcggcggaga
      961 taaatcgggt gtcaaagaat ttgcgacaaa attagtttca gacgccaaca tcgcagtggt
     1021 tgcgatgaat tatgaattag cgcctgattc agaataccct aatcaagttc ttcaagtgaa
     1081 tgaactgatc aaagatttat tagctgaaga aaaagacgac aaattgctag acatggaaca
     1141 attatttttt ggtggagata gtgcaggttc acaaatcgca ttgcaatatg cagctataca
     1201 aacgaatgat gcgtatgcta aacaaatgaa aatcaaacag ctacttccaa aagatagtct
     1261 gaaaggaacc atttcttatt gcggacctgt tgatttgaag ctaacagcac agcagcattc
     1321 ggatgatcga tttatgaagt ttttcgtcaa aaccgttgcg tggtcgttga ttggaacaaa
     1381 agattggaaa acgagtgagc aattgaaaga agctagtgtg gctgaccatg tttctaagga
     1441 atttccgcca acgtatataa ctgacggaaa tgcatactca ttccaggagc aaggtcaagc
     1501 attagaaaat cggttgacag aattaaaggt acccgtgcaa tccttgtttt atacagatac
     1561 aaaaaaagag attacccatg aataccaatt tgattacacg ctaaaagaat cgcaaaattg
     1621 ttaccagcag acattggatt ttgtgaataa gtataagtaa gaggttgtga aaaagccgtt
     1681 tcgttttgag tatgaagaaa cgaagcggct tttcacaacc tctctttcaa tacctttttg
     1741 ttcttgggat aaaactttcc ctaacaatca atcgtgcagg ataagtcaaa atgattggtt
     1801 cgtcccgtat accgtcaatt cgctccaatg ccattcgaac agctgcttgc ccaattgatt
     1861 tcgcattgat ttgaacactg gtcaagccag gggagaggtt tgcgattgtc tcgatattat
     1921 caaagcttgc tacaacaatc tcttctccaa tttggatatt tttttgtttc aatgcccgat
     1981 agacaccaat tgccattgga tcgctcccga caagaatggc agtaggaaga ggggattcat
     2041 tttctaatag attagtcgca agtacctcac cttgtttttc tgtccagcca cctagatgat
     2101 aagacatata tttttctaag tgatgatcga tcataaaatg ctgataggca cgcatcctct
     2161 tttcatttat agaaatctgc ttcccaccga attcatccat atcgacttta tatcccccga
     2221 tataggctat tcttttatga ccattttcta agaacaattc cagtacggtt ttagtcatcc
     2281 gttcaagatc tgcatacacc atatcgacct cttgctggat gtcagggtta tccacgacga
     2341 ctatttgcat gttttgtttt aacaaggcgg taatcgattc tttcgtaaca gtcccaacaa
     2401 cgatcaccgc acctaactga tctagatctt cccattgttt aggattatca gatagattat
     2461 agattcgatt catccctaaa tgcagacgtc gagcttcttc ttccatccct actcgcaaat
     2521 gtttgtaata gatatcctgt tcttcgctcg ctttgccgat cgtagagatc acaccaatcg
     2581 aaggcattct ctttttgata tatttacgag tactgatgtc ataatccaac gcagcagctg
     2641 cctctactat ccgttgccga gtagtctgag ccacatggat cgtctgatcg ttattcaaca
     2701 cacgggaaac tgtagcagga gagacatttg cctttcgtgc gacatctcta attccaacca
     2761 tttttacaca ctcctcaaaa aaataaaaaa tttttattga caaaattgtt tagtaaacag
     2821 tatacaggaa ttataaaaaa aagtaaacgg tttccgtttt gttgggagtg aaatgatgga
     2881 tattacgcat agaaaaacag ataaaaattt catcttattt gatgaaaaga gccgagtctt
     2941 ccatttacga aatacatttc tttcttatct gataaagata gaagaaagca atgtattggc
     3001 gcatatttat tttggcaaac cagtcaagca gtacaaagac aataaaaatt atccccgaag
     3061 agatcgtgga ttttcaggaa atgttccctt aaatcctgac cggtctctgt cgaaagatac
     3121 actaccgcaa gaatactctt ctcatggcag catggacttt cgaacgcccg catcgatcat
     3181 tcagcgcaag aatggatcag acttactgga tctgagatat gacagccatt atattacaga
     3241 tgggaagcca gagatcgaag gcttgcctca aacttatgtg atggacaagt cagaggcaca
     3301 aacgttggtg atttcactta aggatagaga aacagcgatt tatttcgatt tgttctatac
     3361 catatttact gatagagcag tgatcacacg atctgtaaag ataagaaatg aaaccggaga
     3421 aacaatcaaa ctagaaaaag cggctagttt ccagctagac tttgcccata ctaggcgatt
     3481 tgatgaagtc attgctttgc caggagcaca tgtcaatgaa agacagattt cacggcaatc
     3541 tgttttatct ggaacaaaag tatttgaaag ccgtcgagga acgagcagtc accatatgaa
     3601 taactttatt gctttagttc atcatcatac gacagaaaat actggagaag cgatcggact
     3661 tcaatttgtt tattccggca accattcttt cgaacttgaa aaagatcaga tcaatcaatt
     3721 gagagtcgtc ggtgggatca acagccatcg tttttcgtgg gaattgaatg caggccagtc
     3781 ttttcaaaca cctgaaatga tcctttctta ctcaagtcag ggattgaaca aaatgagtca
     3841 gatccaccat gagttgctac gtgaaagaat cgcaagaggt cgtcatcaat ttgctgaacg
     3901 gccgattctg gtcaataact gggaagccac ttactttgac ttcaatagtg aaaaaatcaa
     3961 ggcgatcatt gatgaagcaa aggagctagg catcgaaatg ttcgtattag atgatggctg
     4021 gtttggaaaa agagatgcgg acaattcttc attaggagac tggttcgagt atgaaggcaa
     4081 gttgacaaat ggattacgtg aaatagcaga ttatgctcat agcaaaggat tgaaatttgg
     4141 tttatggttt gaacctgaaa tgatctcggt tgattctgaa ctgtatcgaa cacatcctga
     4201 ttttcttatg caggagccag gtagaatgcc ttctgcttcc agatcacaac atgtactgga
     4261 ttttacaagg ttagatgttc ggcagacgat tgagaaacaa atgagaaaga ttttagatac
     4321 gattcctctt gactacatca aatgggatat gaaccgttcg ctttcagatg tctattcgat
     4381 tacccttgac ccacagagac aaggagaagt agcgcatcgt tatatgctcg ggctttatga
     4441 actacttgaa catttgacaa ctgattatcc ggaaatctta tgggaaggct gttctggtgg
     4501 aggtggacgg tttgatgccg ggtttattta ttatatgccg cagtcatgga caagtgataa
     4561 cacggatgca gtggagcgta tgaaaatcca atatggaaca agccttgctt atccgatttc
     4621 gtccatcaca gctcatgtat cagctgtacc aaatcatcag accgacagat ctacttcgtt
     4681 gaaaacaaga ggagaaacag ctatgagcgc tgtttttggt tatgaactgg atttgacaaa
     4741 gctgtctcct gaagaaaaaa agcaagtaaa agaccaaatt atatcctatc aaacgattcg
     4801 tccggtgatc cagtatggcc attattatcg tttggcgagt ccttttgaag aaaatattgc
     4861 cgcatggatg tttgtgtctc ctaaacaaga tgaagctatt gtgtttttag ggaggatact
     4921 agcttcagct caaccagcgt tccatgaagt atatctgatg gggttagatg atgaggcact
     4981 ttatcaggaa cagacctcga agcggatatt ttcgggggcc gaattgatga cagttggact
     5041 ttacttcccc gattttcaag gtgatttcca aacagaactg cttcatttca aaaagttatg
     5101 agagagaagg aaaaaagtat gaaagcaaat ataaaatggt tagatgatcc acaagttttt
     5161 agagtgaacc aatcaaatgc acatagcgat catgtttgtt atgcaacaag ggaagaagta
     5221 gaaaccacat cctcccttaa acagtcttta aatggatcat ggcgtttttg ttattcgcca
     5281 aatccttcgg tacgaccaaa aacattttat aaaaaagatt ttgatgcctg ttcttttgat
     5341 gaaatcactg ttcctagtca tattgaatta gcaaattatg acaagatcca ttacatcaat
     5401 acgatgtatc cgtgggaggg acatgtattt cgccgtcccc ctttcacgac aagccctgag
     5461 aaagaggcgg aagggatctt ttcagctgct aaagacaatc cggttggttc gtatttgacg
     5521 acttttcgat tagaagaagg tctaaaggga aaacaaatct cgattcattt tgctggtgta
     5581 gaacaagcaa tgtttatttg gttgaatggt cattttatcg ggtatgccga agattcgttc
     5641 actccgtctg aatttgattt aacaccttat ttggaagaag aaaatattct agctgtggag
     5701 gtttacaaaa gaagtacagc tgcattcttg gaagatcagg actttttccg attttttggg
     5761 atcttcagag atgtcttctt agttgctcga ggacaaaacc atttagaaga tctttggatc
     5821 aaacctcgct tagatgacaa atgtcaaaat ggcaaacttg aagtagattt gtcttttgag
     5881 caagtagcca aaaacagtca gctgaatgta gcgatactag atcctgatgg gaaggaaatc
     5941 tatcaacagc aatatccgtt aaaagagcaa atgacgattc gcatcgatgg atttgaaaat
     6001 gtggcactgt gggggcattt tcagcccaat ttatataagc ttgaattgga gacgatagac
     6061 gaagaaaaaa gagtaacgga gtatattgac tattccttcg gttttcgtaa aatcgaaatc
     6121 aaagataaaa tcatctactt caatggaaaa cggatgatta tcaacggggt aaatcgacat
     6181 gagtggcatc cgaaaaaagg acgcgccatc actatagaag atatggaaaa agatttggct
     6241 atcataaaaa atgcaaagat caacggcgta cggacatctc attaccccaa ccagatcact
     6301 tggtattatt tatgcgacca agcaggaatc catgtgatgg cggaaacgaa cctcgaatcc
     6361 catggctcat ggcaaaaaat gggcgcattt gagccttctt acaacgtacc aggtgcagta
     6421 cctcagtggc aggcggctgt tcttgatcga gcaaaaacga atttcgaaac attaaaaaac
     6481 catccaagta ttcttttttg gtctcttgga aatgaatctt atgcggaaga aaacattcga
     6541 caaatgaacg attacttcaa aaaagtagac cccgatcgac ttgtccatta tgaagggaat
     6601 tttccaaatc cagcattcga agattcgatt tcagatgtaa aaagctgcat gtatgcgcca
     6661 ccagaagaga tcattgacta ccttgaaaat aaagcggaaa aaccgttcat cttatgtgaa
     6721 tatatgcatg acatggggaa ttcgttaggt ggaatggatt cttacatgac gttgcttgat
     6781 cgttatgaaa tgtaccaagg aggctatata tgggatttta aagatcaggc gatccaagtg
     6841 actgatccaa tcacaggcag agaagtgatg cgctatggcg gtgactttga cgataaacca
     6901 agtgactatg aattctcagg gaatgggatc gtttttgcag atggacaaga aaaacccgcc
     6961 atgcaggagg taagatatta ttatgaaaaa tacagtaaat aaaagtcata tggatacgga
     7021 aaaagttgca atcgtcttcg gcgactgtac attaggtgtc aaatcgggga atacgcatta
     7081 tattttttct tatacaagag gcggactgga atcgctcaat aaaaacggaa aagaatggct
     7141 ataccgggaa acacttccag ttttttggcg agcaacgacc gataatgaca gaggtagcgg
     7201 atttggattt aaatccggta tgtggttagg agccggattg tatccaaaag tcaaagaaat
     7261 agaagtactg gtggacaata aaaagatcga tttgccgatt gctccagtaa ataatcaata
     7321 ttcaagtaat gaatacgctg aaacggttga aatcattttt acactggaaa tcaacacagt
     7381 accaaaaacc caagtgacaa tttcctacca aatcgctgga gagggtaaaa tgcttgtcaa
     7441 tatgcattat tatggacaaa aaggattacc ggaattaccc gcattgggtt taaggttcat
     7501 tatgccaacg aaagcaaaat tttttgagta tgaaggccta tcaggggaaa catatccgga
     7561 tcggatggct ggtggtgtcg aaggtattta taaagtggca ggactccccg tttccccata
     7621 ccttgttccc caagaatgcg gcatgcatat ggaaacgaaa tgggtagaac taacaagaaa
     7681 tcaaacgttg gataatacgg ataaagatca agaagatttt tcgttgcgat ttgaacagca
     7741 ggatacgcca tttgcttttt ccgctttacc attcaaagca gaagaactgg aacaggcaac
     7801 acatatagaa gaactaccgc cagaaagacg aacggttctt acgatttttg caaaagtaag
     7861 aggagtcggg ggtatcaata gctggggagc ggatgttgag tcaccttacc gaatcagcag
     7921 cgaagaggac catgaaatcg gctttattat tcagtagcaa tctttagatt attatttgaa
     7981 aaacggagtt tacaagacaa agctgcttta ttctatatgt catgaacaag agagtaaaaa
     8041 agttctc
//
LOCUS       DAHHZJ010000128         7943 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.130, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000128 DAHHZJ010000000
VERSION     DAHHZJ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.130"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(<1..830)
                     /locus_tag="NBL02_002203"
     CDS             complement(<1..830)
                     /locus_tag="NBL02_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCD2572880.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     "
     gene            complement(1054..2994)
                     /gene="thrS"
                     /locus_tag="NBL02_002204"
     CDS             complement(1054..2994)
                     /gene="thrS"
                     /locus_tag="NBL02_002204"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCD2572881.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3331..3510)
                     /locus_tag="NBL02_002205"
     CDS             complement(3331..3510)
                     /locus_tag="NBL02_002205"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HCD2572882.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3557..5380)
                     /gene="cadA"
                     /locus_tag="NBL02_002206"
     CDS             complement(3557..5380)
                     /gene="cadA"
                     /locus_tag="NBL02_002206"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HCD2572883.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5658..7937
                     /locus_tag="NBL02_002207"
     CDS             5658..7937
                     /locus_tag="NBL02_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCD2572884.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
BASE COUNT         2319 a         1697 c         1443 g         2484 t
ORIGIN      
        1 tattcttgga ttgcttctcg tgtttttttc actttttcgc taagcttttc gtttttagcc
       61 atatagaaat aaaaaatgat atcgatcgta gcaaattggg ccaaaagtga attagttgcc
      121 gcacttcgat tcgtgatttc ttttggtcta gatgtttgta cgagtacatc ggctatcttg
      181 ctaagcgggt tatttccaaa ttgcgtcaac gcaatgattt cgatattcat gcttttcgcc
      241 agctcggcta atgcgactac atctgcactt ctacctgagt ttgaaacaag ccaaagcact
      301 ttctttttct cattgctgac aagttgcggg agcaagacgt ggatatcttt ttcaaaaatg
      361 attggcttac cgacacgaga ccatttttgc agaatatctt ctactactaa agaagaggcg
      421 ccaaccccat ataaaaaaac agtctctgcc gattccaaac actctacgac tttttggatc
      481 gtttcttctt ctagaatatc tactgtttct tttatcgcta cttgtgcatt gttaccaagt
      541 ttgtttttga tagagtaaaa ggactcattt gcttctatat cactaaaacc ttcatcgatt
      601 ccagtctgta gttctgttga aatcgtgatt ttgaagtccg tcagactttg aaaaccgatt
      661 tttttggaaa aacgtacaac tgtcggcgca ctcaacccgg aagcttctgc taattcatta
      721 gctgtcattg tagggacctt ctcaatatta ccgataatgt agttggccaa ctttttttct
      781 gctgaagaat aatcttccat catactttca atacgactaa ttacgctcat aagttcctcc
      841 acccttttaa tgatagtcta tgtaagtata ccatatcaga cccttattca cggtcgtata
      901 ctggataaaa aacaaaaaaa caatccgccc aacagaatat cttatcccat caatcccaca
      961 gattctattg agcatctgtg tctttgagga atacctgtta tctattgttc ggattgcttt
     1021 ctatctacta cttttttaca ggaaatttac ctattagttt tctctgctgt aatttttgac
     1081 ttctgcttcc atagcatcaa taaagatatt caaatcttct acagctgttt ctttacttcc
     1141 ataacgacgt acgttcactg ttgcatcttc caattccttg tctccaacaa ctaattggta
     1201 agggatcttt tgtgtttgag aggcacgtat cttatagccc attttttcat tacgatcatc
     1261 aacttctaca cgcaacccct tcatttgcaa acgttctttg atttcataag cataatcgcc
     1321 atgtgcatca acagaaacag ggatgattgt tgcttgtata ggtgccaacc atgttggaaa
     1381 agcgcctttg tatacttctg ttaaataagc gacaaaacgt tccattgtag aaacaattcc
     1441 gcggtggata actactggac gatgcgtatt ttccccgtct tctccaacat atgtcaaatc
     1501 aaagcgctct ggcaataaga agtctaattg tatagttgat aatgtttctt ctgtaccaag
     1561 agctgttttg acttgaacat ctagttttgg tccgtaaaag gctgcctcgc cttctgcttc
     1621 aaagtaatct agttctaatt catccatagc ggctttcagc attgcttggg ctttttccca
     1681 cattgcgtca tcatcaaaat atttatctgt attgtttgga tcgcgatagc tcaagcggaa
     1741 acgataatct gtaatattga agtcagcata tactgccacc atcaattcaa gcgtacgttt
     1801 aaattcatct ttgatttgat caggacgaac aaatgtatga ccatcattca acgtcatttc
     1861 tcgtacacgt tgtaaacctg acaaagcacc tgatttttca tagcgatgca tcattcctaa
     1921 ttcagcgata cgaattggta attcacgata agaatggatc gtgtttttgt agaccatcat
     1981 gtgatgaggg cagttcattg ggcgaagaac aagcatttcg ccatctccca tatccattgg
     2041 cgggaacatg tcttcttgat aatgatccca gtgtccagat gttttgtaca actcaacatc
     2101 tcccattatt ggtgtatata catgttggta acctagacta acttctttgt ctacgatata
     2161 acgttcgatc gtgcgtcgga ttgttgcccc ttttggaagc cagaatggca aaccagaacc
     2221 tacttctggg gaaaccatga acaagtcaag ttcttttcct agtttacgat ggtctctttc
     2281 ttttgcttct tcacggagac ggatatattc tttcaaagct ttcttgtcaa aaaaggctgt
     2341 cccgtataca cgttgcatca tttgattgtt tgagtttcca cgccaataag cacctgccac
     2401 tgataataat ttaaatactt gtatacggcc agtggatgga acatgtggtc cacgacacaa
     2461 atcgatgaat tcgccttgtt gataagcagt aatcacttct tcttctggca attcactaat
     2521 cagttctact ttgtacggat cacttgcaaa aatttctaat gcttcctgtt ttgaaagaac
     2581 acggcgttcg atcggcagat tttctttgac gattttcatc atttctgctt cgattgccgg
     2641 caaatcttct gcagttactt ggttttgtcc attatctgta tcgtagtaaa accctgaatc
     2701 gatagctggt ccaacgccaa agtggatatt tggatagagg cgacgcatgg cttgggccat
     2761 caaatgagct gctgaatgac ggactaagcc aagtgcatct tcatggtctg gtgtgatgat
     2821 ctcaatactt gcatcttcct cgattggacg agtcaaatca acgagttttc cgttcacttt
     2881 accagctaaa gctttttttg ctaagctttt tgaaatgctt tcagcaattg ccaatgttgt
     2941 tgttccagac tcaaattctt tgactgcacc atctggaaaa gtaattttta tcataatagt
     3001 ttcctccttg aaatgttttg attgatcaga tcatcaagct gaaaaacaaa aaaatcctag
     3061 catctcttga aaagagatac taggacgaaa agttcgtggt tccacctaat gttcaaacgc
     3121 aaatgcgttc ctcaagcgtt gtaacggtcc gaccgccctt gcttcaacaa gggtttcgaa
     3181 agtggtcatc ctttggtttt ctcctggaaa agtttcagcc tagcttttcc tctcttatcg
     3241 aagactctca aattcagttg gctttctcat caatcaatta tgtgtttatt taaccactaa
     3301 taaatagaaa aatcaagtgg gaaactaaac ttattttttc cattccccat ttacaccgta
     3361 tgttcctttt ttcatctcat tgatgatgac atggatgtgt tcttttggtg cctgcgtgtt
     3421 tttatgaaca gcttccgtga tatctttcat catattttct aattgttccg gactgcgacc
     3481 ttctactaat tcgacatgta cgaatggcat aatgctctct cctctttctg atcgttcttt
     3541 tttcaactcg attctatcat actatgtttg tcttgggtac tgaatcagga aaaatggctg
     3601 gatctttttt tgctctcaag gcactcaaaa tagagaccgt atccacgact tcttgcaaaa
     3661 cagctccgaa taatgcgggg attagtcccg tacttgcaat taacatcaaa aaaatacaaa
     3721 taaagatgcc aatcagtaca gactgtttcg ctattctcat cgtatctctt gaaatcacta
     3781 ctgcttttgc tacacgcaag agatcatctt tcaagatgac cgcatctgca ctttcactag
     3841 cagctgtaga accatgagcc cccattgcaa taccgatatc tgcagcagca agtgctggtg
     3901 cgtcattcac gccgtctcct accatcgcaa caggtctttg atctttaggg atactttcta
     3961 aaatggtcag tttatcttga ggcaagctac gggcatgtac agaatctaat tggacttttt
     4021 ctgcaatcgt ttgagcaatg gcagatccat ccccggtgat catcatcaaa tttttcacat
     4081 caagttttct tagctccttg attgtctttg ctgcttctgg gcgaatctga tccgaaaata
     4141 ggatcgttcc tgcaaaagat cgatcaattg ttacatagat tgctgtattg gacgtttcta
     4201 tttcttcagc tgaaacaaaa gaggcacgtc caatctttac ttcatgatta ttgactactg
     4261 cttggattcc ttgaccggtc acttctttca gttgactaac aggatacagt tggctgtttt
     4321 tcacgttgtt cactaacgaa cgagctaaga tatggctaga ttcctgttct gcactagctg
     4381 catacagaag tagctcttct ttttttattc cattgactgg ctgaatcgcc tctacttcca
     4441 atatcccttt ggtaagcgtt cctgttttat caaaagcaat cgtttttgta cgtgcgagct
     4501 tttcaatcgt tgttccagtt tttacaacga tcccgttttt acttgaacgg ctcattcctg
     4561 caactaaagc aatgggcgca gctagaatca gtggacaagg tgatgccact accagtacct
     4621 cagcaaatcg taaagggtct tttgagaacc accaagaaat accaccgatc aaataagcaa
     4681 tcaacgtgaa agggactgca taacgatcag ctaaacggac aaatcgggca ggcttttctt
     4741 ttgattcttc gactagttta ataatcattt gatactggct gtctgcggca cgtttttcag
     4801 cgatcatttg gatcgatccg tctccattta aagaccctga cataatctga tctccaggtt
     4861 tcttggtttc cgggcgggac tcacctgtca aagaagattc atcgaccgaa gattctcctt
     4921 tggtcactcg accatctgcg ggtacaagtt cgccaggctt gactagtaac tggtcatcaa
     4981 tatttatttc atcgattact atatcctgca attttccttc tttgaatcga tgggcaattc
     5041 ttggggtgtt atctaacaaa gactgcagtt ctttacccgc tcgtttattc gcgtaatcct
     5101 ctagactatc tccgcccgta agcatgatca atatcatcaa actagcccag tattctttga
     5161 cggctaatgt tgcaaggatt gcggtaatcg ctaatacatc tacgccatat cttcctgaac
     5221 gtaacgtctt gatcatttcc aaaaacatca agaaggcgag aatccctcca gtaaatgcga
     5281 caatcgcaaa tgcaagggcc ggctggtgaa aaacaaattc acttaataaa gctatcccac
     5341 caataagcaa agtaagtaaa aattttttga aatgtgtcat tgttttttct cctcttttat
     5401 gtgacttaga gaggttagta ataaatttcc agctgcctct ttcatcttaa gcatagtcct
     5461 tgactagcac tttttaaaac aataggcctt aattgaaaac aactatcact atcagcgttt
     5521 ccaaaattca tcagttctac aaacaaaaat attcagcaag gattactttt tcgattcatc
     5581 agttaaaaag tacctttttt cgaaaaacta tgctatactt ttaattcgtt atttacggcg
     5641 gaaggagcaa acatgcgatg aatgagaaac aactagaaca aacgcacgtg gatgaaacga
     5701 tcgaactaat ccagatcgaa caaaatatgc tggatacaaa acaacaagaa ttgactcagg
     5761 aaatgaatca ttcgacaaaa gacacagcta atcacaagat tcgagctggt tccaatgaag
     5821 ctttttatga atctgctgtt gaatatcgcc aacatgagca ggaattgctt ttgaaatacc
     5881 atactcttga agcacagcaa aaacgcctgc aaacgttgtc tgtcatgaaa gatagtcctt
     5941 attttgcccg tatcgacttt aaagaagacc gtgaaacaga aacattatat ctaggtatcg
     6001 cttctttaag agatctggca gaagaaacaa tcgttattga ctggcgcgca ccaattgcca
     6061 atctttatta tgaaggtgag attggtcccg cattttatga aactgaaaca gatagaatag
     6121 atgtcgatct gcagttgaaa cgacaattta aaatccaaga aggacgtatt ttatctatgg
     6181 tagatacgtc ggaagtaatc aatgatgact ttttgctaga gatattggat gaatcatcaa
     6241 gcaaccatat gaaaaacatc gtctctacga tacaaaaagc acaaaatgca atcattcggg
     6301 acacaacaag taaagtaatg ctgattgaag gaatcgccgg aagtggaaag acatccgctt
     6361 tgcttcaacg tattgctttt ctgttgtatc ataatcgcaa gtggctcgag gctgaaaacg
     6421 ttcttctatt ttcacccaac catttgtttt ctgattacat ctcgactgta ttgccttcat
     6481 taggtgaaag cggcgtgcct acacagacat tcaaaaatta tttggtgcag ctgctgccag
     6541 agttttcttt actagatgaa cagcaacaag aaagtggttt tttgctaggg gaaaaagatc
     6601 cgatacaagt gatgaaaagc ggattgacct tagtcgatca gattgatcgc tatatccaag
     6661 gcatcacaaa ctacggtccc ttgttccgtg atatgaaaat aaatggccgt acgatcctat
     6721 caaaggaatc gattcgccaa tggtataaag aaacaaatga acaattacct ttacatcata
     6781 gactttctct attacaaacg aagctcctga aaaaattagg cggtctgcaa aaagacgaaa
     6841 cacgtcagca atgggtcaaa gatctggcgg aagaacagct ccaagaacta tacgcaacag
     6901 atccaaatct tgaatataca gaacaaaagg agcgttcatt acgtaaaaaa ctggccaacc
     6961 aaatcgtacg taaaagattc cgtcgaatcc atcgagggat catgaattat caatttgtca
     7021 atataacgaa acaatatctc cactttctac aaactgtgcc actttctctt ctaacacgac
     7081 ataaaatcag cgagaccagg tggcaggaac atttgacaac tgttcgtaca cacttgcgtc
     7141 aaagagaatt gcgtcaagag gatgctgtac tattcttttt actgatgcgc cgaacatatc
     7201 cagtgacggt tacacacaaa gctcgctata tctttattga tgaaatgcag gatttcgcac
     7261 catcacaagt ggcattgctg caagccttgt tcccaaaagc aaatttgact ttctgtggag
     7321 atctaaatca aaatgtcttt ggaaatgaaa cgatcgttgg atcattggaa gaccttttcg
     7381 tggaccgtga agtcactcgt ttccaattaa cgacaagcta tcgctccaca aaagaaatca
     7441 ctgattttgc taatcatttt attgtgaaag aacatcaagt agaaacaaca gcaagaaaag
     7501 gagaccttcc tttgctcatc caaggaacaa cagatgatat caaattgaat tggttagcag
     7561 ataccattcg aacgactcag ggaaaagccc ggtattggcg tacagcaatc atagctaaaa
     7621 cacaagaaga atgcgaagct ctttatgctc aactgccaga cgagttgaaa caacaagtcc
     7681 agctgatttt aaatgagagt gactttatga agcgttccat tattttactt ccagcctttt
     7741 tagcaaaagg tctggagttt gaccgagttt tcttgtggga tgtagatgca gaacattttt
     7801 cttctcagca agacaaattg atcctttaca ccatgtgtac acgtgcgatg catgaattga
     7861 ccctttttac ctccaataat gaaagtcctt tgttaaacaa tatggatatt tcatcttatg
     7921 agaaaaaagt actgtaagta aaa
//
LOCUS       DAHHZJ010000129         7845 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.131, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000129 DAHHZJ010000000
VERSION     DAHHZJ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.131"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            94..2421
                     /locus_tag="NBL02_002208"
     CDS             94..2421
                     /locus_tag="NBL02_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCD2572885.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2547..2876
                     /locus_tag="NBL02_002209"
     CDS             2547..2876
                     /locus_tag="NBL02_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HCD2572886.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            2916..3920
                     /locus_tag="NBL02_002210"
     CDS             2916..3920
                     /locus_tag="NBL02_002210"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HCD2572887.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3917..4363
                     /locus_tag="NBL02_002211"
     CDS             3917..4363
                     /locus_tag="NBL02_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HCD2572888.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            4513..5328
                     /locus_tag="NBL02_002212"
     CDS             4513..5328
                     /locus_tag="NBL02_002212"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCD2572889.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            5534..6706
                     /locus_tag="NBL02_002213"
     CDS             5534..6706
                     /locus_tag="NBL02_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCD2572890.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6868..7362)
                     /locus_tag="NBL02_002214"
     CDS             complement(6868..7362)
                     /locus_tag="NBL02_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572891.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(7359..7553)
                     /locus_tag="NBL02_002215"
     CDS             complement(7359..7553)
                     /locus_tag="NBL02_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572892.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
BASE COUNT         2661 a         1462 c         1547 g         2175 t
ORIGIN      
        1 gccccaaact ttctatacat caataaaata ataaaaatta aatttagaat aacgtgtaaa
       61 ggattatttg atgatttttt attgagagga gggatgaaaa tgattaagaa aactcaaaat
      121 aaaatccaac gtatatgtca aaaacatttt gtttctagac tcaaagctgt atctaaaaga
      181 aaatacttac atcccattat gatttcgatc atcttgattt ttactttctt agggagctta
      241 tcaagtattt atgtaaacgt ttatacagaa acgtcaaatt ttgctaacca aacgacagag
      301 gagacaaata gtccacccta ttctgaaata cagtccatag aaagcaccaa tcagtctagc
      361 aacaacgaga caacctcaac cacatcctcg actcaactga ttgatccgac agaaaaagaa
      421 atcacagcag caaaagcagc aggggagaaa gcttattcag aaacaggtag aagccagaca
      481 tttagtactg ttgcggccac tgcgaatttc aacggacata ccataccaga tggatataca
      541 gtgatatatg ccaaagatgg agtcattgtt gcggttaacg gatcgatgag tgcggataaa
      601 actgcgttac aaggcgtaac caaagcaatc gcttttattg ataaccgtcc agatatccat
      661 aatctgttta ttggctccac agtaaatggt actactatca cattcccaaa aagtatctat
      721 atgggggtgg ctaccgctgg aaatacgatc gattcagtta cacccaatgc ggataaaagt
      781 tattctttta caccctcaac atggggaggg cttcctgccg gaggaggtat gagtactttt
      841 tcaatcggta tacagttagc aaatgatatt ccattcacgg ggtactcgat caaaaatggg
      901 cagatcgttg atggtaaggg aaatgtttca aattttccaa cctatcaagc agcaacggtt
      961 caggtcaatg cgaatcggac aggaactttt tcttatacag tcaatggtgg agcagcaaaa
     1021 atcggaacgt acgggactac attatctgga atccatgtgg gtgacaaagt ggttatcact
     1081 ccaacagcag taacgggcta ttcttttgca caaacaaacg gttctttcac agccctagaa
     1141 agtaacgtcg atacggtgac ctatacaggc aaacaaacac caatcactat taagtatcaa
     1201 gactattttg gacagatgat tgctccttct aaaataatga atcttactta cggaagtgct
     1261 gcacaagacc tgacgactaa tacgcctact atcaacggat atacctttac agctgtttca
     1321 gcgatagaaa caaagaacca atcagcaatg agtgttttag ctagtttaga tacaaatgga
     1381 aatgtaatcg taaaggacgc aaatggaaaa cagatttctg aagtcatcct atattataag
     1441 acaaatgcaa cagtatcaat caatgcaaat ggcagtaagt actatgatgg tctttctgtt
     1501 gtaccgatcg ttaaatatgc ctttaatgat aaaacagaat caacatccct tttaaacaat
     1561 gatctcagta ttgatcaaat cacatggaat ccaactgatt tcaaagcgat gaatgaaaag
     1621 ggagttgaag taactgcgcc tactgaaatt ggaacctata cgtcttggca attgacaaaa
     1681 agcggactag ctaaactagc tgccagaaca aactatttat ttactatcgt gcagacatct
     1741 gatgtctata cgattaagca aatcagcggt gcggtagagc tcagtggcag taagacatat
     1801 gatggaaaag caggtgtccc gtcaatccat gtcaagctag cagagggtgt cactagttcg
     1861 cttactccag tacaagtggc acttagcagt atggattaca cagcagatgc acaatcagca
     1921 aaaaatatgg ttaatgctgg cagctataca attaaactaa cgaatagcgg aatagacaaa
     1981 gtcaaagcag caaatcccaa gttgtcattt actgatatag ccaacacatc aggaacttat
     2041 accatctata aagctaacgc agtgattact gtcgatgacg ctgcctttaa ttatgatgcc
     2101 caaagtcact cgatttcggt tggaaatctc cacgttaccg gtgttgtctt aggcgaatca
     2161 ttggattata ccctaacagt taacagccgg acagatattg gatcacaaat agtaacgatt
     2221 agtttaggcg aaggaagtgt aaacaataat tatacgatta tcgcaacaga taccgctaag
     2281 ttgacgatca atccgactct cttgcttcct tcgacaggtg ggataggtct actgcccttt
     2341 gtctttttag gactgatctt tattttttct ggcttctttt attttatcca tcgtaagaaa
     2401 aaagcaggtg aacagcgatg aaaaaaacaa aaaatgtacg tgtggatata tttgctgttc
     2461 tcttttttat cctactggtc ttttttatag gttctccgac tgtgtcagct agagaatcac
     2521 ctattacatt tactttgcat aatcgaatgt tcgaaagcga taagatgcca agtatcagtc
     2581 aaaacgacgg aataaatatc ccggataact ttcaaggagt aaaagggttg aacggcgtta
     2641 ctttcgctat atatgatgtc tcggatgaat tttacaagct gcggtcagaa ggaagtagtg
     2701 tggaagacgc acaaagaaaa ctagcacaaa aaagtgatag tgaaaaaata ctggcagaaa
     2761 atgtgacaaa aacagtagac gaggaagagg gcattgcttc ttttagtgca tcggacaaag
     2821 atgagcaagg tcgtgacgct gtttaccgat ttacggaaat aaagacttca gattaagtaa
     2881 aagaaaaatc agcaccattt gtcgttgtgc ttcctgtgac agattcatct aatcataaat
     2941 tgacgaccat ccacttgtat ccaaagagcg aacaaaaaat acctgctgca ctcacattaa
     3001 caaaaacagt agagaataag cagacagatt ttgcagatgg ggataaagtc ccttatctca
     3061 tcaccgtaac aattcctgag aacatcaacg agataggaac ctatacgatc aaagatacag
     3121 ctgatcctca gttatggcta gaaccagaaa cgatcgattt tttgattggt ggagacaaga
     3181 ttcatacatt tcacacacaa aaaataaaac atggttttgt cttatcagat tctggaaaag
     3241 atcttagttc gtttgctgga aaaaaattaa cgatccgtta ccaaatgaca ttgaaagaaa
     3301 acagtgaaaa aacgacattt gataacgatg tacgtttgac gactgacaat caaacagtag
     3361 aaacacatct tgccatccaa acaggaggca aacaatttgt caaagtggat cggcaaacga
     3421 aacaaaaact agcagatgct caatttctag tcaaaaaaaa tgaccaatac ttagcgcaag
     3481 aaaacgggat aaatcattgg gtagccaaag atgattcaag agcaacgatt tttacttcca
     3541 gtaaagatgg aacattttct gtccatggat tgtcttttgg aagttacgaa ctagtggaga
     3601 ttaaaccgcc taaaggatat atattgagtc aatcaaccat tctctttgaa gttgaaaaag
     3661 gcagttatga tccgcttcat cctataccat tggaaatcat caacgaacca aaagcaaatc
     3721 cgacgaataa tggaaaaacg ccagttcaga tgcagacaaa tggtccatca aatgaaaaaa
     3781 atggaggaag ctttccaaaa acaagcgaag agatgcttgg aggtttctca attttagggt
     3841 tattgctagt cctttcaaca gggacagctt ggttttataa aaaacaacaa aaaggaaaca
     3901 ataggaggga aataaaatga aaaacatcaa aacaaaaata tggacgtttt taggaactgc
     3961 tatcatgctg cttcctttcg ttctcggcct aggaactgca gaggtttctg cagccgtatc
     4021 acctacacca gaaaatgtta cggttaattt gcataaattg aaattcactt cagcaccaga
     4081 aaatcaaata aacaacggaa cggaattgac atttccgaat agcgaaccgt tgaacggcgt
     4141 tgaattcaac gtatatgaca tcactgctac gtactatcca agtaaagaca cagcagttcc
     4201 agcagacgct acacccttcg caagtgtaac gacttctggt gaaggattag ctaacctgac
     4261 tcttccaggt aaatctgatg ggaaagatgc tgtatacgtc tttgtagaaa caccaaagcc
     4321 cggtgtcgaa acgtcgccaa atatcgtgtt aagcttaccc tagaccctga caaaccttta
     4381 gacacgattc acctatatgc taaaaatgtc atcaaagaaa gcaatatcaa aatccagaaa
     4441 gctgggaact tttcaggggt agataagtta gcaggggcgc aattccagat caaaaattca
     4501 gagaatcaat tcgtgaccgg ttttgatgaa aacactggtg tcgctctcta tgatcctgct
     4561 gagtcagcgt tgacattcac aactgatact gaaggatcca tcagtgtcaa tggattaata
     4621 gacggcaatt atactttagt tgaaacaaaa gcaccagatg gttatcaatt aaccaatcca
     4681 caaaaagaaa ctacttttat agtaaaaaac ggaaaattgc aacaaacaac cggtatcatc
     4741 catggcgcag atgaagccaa tcttgggaat ccaaacggtg tagtcaatcg ggaaaataca
     4801 actacggata acacgattac tgtagaaaat gtccaaaact acggtgattt ccacttcatc
     4861 aaacaagatg tgaacacgca agaaaaatta gccgatgcag aattttctat tctaagagca
     4921 aaagatccaa ctgttaaatt aatgaaaaaa atagcaaacc ttgcctcagg agaataccca
     4981 tatgcttggt cagatgaagt cacaggttct gactgggaag aagtgaaatt gatttctgat
     5041 tcagaaggga aattttcaat cgaagggctg aaacaagggg catactgcct tcaagaaaca
     5101 aaagcaccag aaggctacgc attacctaag aatgaagctg cgtacactga atttacagta
     5161 gatgatgatc caacaacttc tgatgaaaca acgatcgata accaacctaa aggaatcttg
     5221 ccttcaactg gtggtatggg gatcatcgta ttcatcttgg taggtactgc tttagttgga
     5281 ggagcagtga tctatttcaa aaaacgccat caagaaacag aagcttaaac tattggaaac
     5341 aaaaatcata cgataaataa acgatagtga ttcaaatggg gagaaaggtt gtgacaagtg
     5401 gtcggtctct tggtattcgc tcaacaaacg gcaaaatagg aggaactatt ttgccgtttg
     5461 cttcgctaat actcaggtca aggcgctgat ttcacaacct cttcatatac atattagaaa
     5521 ataggtggac atgatgaata aaagcagaat aagaatactg gacatcttca tggcaatcat
     5581 tttagtcgtt gggatcggga tcttttctta tccttttgta gaagattcac tgaatgattt
     5641 tcttgcccag caaatgatca tccattatca aaagcaagca tcgaataaga attcagagga
     5701 aataaaaaaa cagcaggaaa aaatgacaaa gaaaaaccaa cagttggcag agcaaaacgt
     5761 atcaccaggg ataggcagtt tcaaccaagc agttgatgca aaagcattaa aagatttacc
     5821 tagtaatgcc ttttttatgg aacatatgct tggagtcatc gaaatcccaa aaatcaatgt
     5881 cagcttacct atattcgatc agacaacgga aatatttttg caaaaaggaa cgtcattatt
     5941 ggaagaaagt tcttatccga ctggcgggaa aaacacccat gccgttcttt ctgggcatcg
     6001 tggactccca gaagcaaaat tatttacaga tttgcctaag ttgaaaaaag gagaccagtt
     6061 tttcatccag atcaatggaa agacattggc gtatcaagta gaaaaaatcc aagtggtctt
     6121 acctgatgaa gtagattctt tgggtatcca aaaaggcaga gatctcgtga ctttgcttac
     6181 ctgtactcct tatatggtca atacacatcg actgcttgtg acggggcatc gtattcctta
     6241 tcaagcaaaa gaggcaaaaa aagcgatcca aggaattgat caatggaaaa aatggaaatt
     6301 ttttatttgg tttatcggta tactacttgg atcaatcggc ttagtttggc tactcatcgc
     6361 ttatttagat tctttggcca tcgctaagcg aaactatcct ttatcttttt atgtaaagaa
     6421 cacaaacgga cgaccaatcg aaggaatggt cttttctgta aaaacgctca acggcaaaca
     6481 ctatattact agagaaaaag ttccatttgt gaaagcttct gatgaatacg gactagtgag
     6541 attttctgat ttaaaaggca ggaactatcg cttgcaacat gaggagctcc tgcttaaaat
     6601 ccatgtaaaa cataaacatt cgaaacagtt ttcaatgaaa ttgaaaaaag gaagatataa
     6661 actgcgaaaa gaaaaagagg tgtactattt gattgagaaa gaatgaatga agatgtccaa
     6721 aaaagcagag ggaacagact aaatgaaaaa agagagaagc tgacgaaatg gacgtgtaaa
     6781 ctgttatttt tggtttacta tctggaacgc catcttctat ttttttatgt ttcagaagaa
     6841 ttttattatt atagtataac ttttgaatta gtttccataa catttggagc aaggggttaa
     6901 accacgagct tgagcttcgc ttaaagtggc aggggtatac gtaccatttc cacatttatg
     6961 tgtatggtat tttgaaccgg tcggtgtgac atagacagtt tgttcattat tttgctcagg
     7021 agctgcggct tgtgcctgct gagaggcctg ttcagctgct gattgctgtc gcgcctgttc
     7081 tgcttgttgc tgtgcgattg cttcttgcct cgcctgttct gcttgttgct gtgcgattgc
     7141 ttcttgttgc gcttgttctg cttgttgtcg tgcaatttct tcttgctttt tccgttcttc
     7201 ttctgcttct tttgctttga ttgtggaatc aattgcagca agacggcttg tcaactcttg
     7261 tttgttttca ggtagcgctt ggatagctgc tgaagcagtt gcatagtttt cttttgtagg
     7321 atttgcttca gcttgtgaca aagcagattc cgcttctgtc actttttttc tagtgcagct
     7381 ttttccgctt tttgtttctc ttcttttttc ttttcaattt gttcttgttc tttcgatgaa
     7441 tctgccgtac tttcggagat cgtggtactg ctgctggaag cctgatctga acttgtgctt
     7501 tgagtagtag acgtagtagc accacaccca ctcaacaaac ttcctgcgac caaaaacaat
     7561 gcaactaatt ttttcgctaa atttcctcct taagtaaaaa acaaccgttt tcaataaatg
     7621 tccttttcaa ttgtttactt acttgttata ataattgtgt tatgaatagt gattgaggaa
     7681 acttaatcta atcccgtgtt gatacacggg attttttgct atagttctgt tattaagtat
     7741 gcaataataa aatatgtacg ttattttatt attagattat tgacatttat ataataaacg
     7801 attaaatgat tcatatcaat agaatactta ggaaaaaaag agaaa
//
LOCUS       DAHHZJ010000130         7823 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.132, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000130 DAHHZJ010000000
VERSION     DAHHZJ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.132"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..365
                     /locus_tag="NBL02_002216"
     CDS             <1..365
                     /locus_tag="NBL02_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572893.1"
                     /translation="EKVRYKLPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKP
                     YSLFAMYYTFSSFLFLEMLSDKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKR
                     EFPNSGKAWLINIGQFFI"
     gene            378..2396
                     /locus_tag="NBL02_002217"
     CDS             378..2396
                     /locus_tag="NBL02_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HCD2572894.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(2456..3172)
                     /locus_tag="NBL02_002218"
     CDS             complement(2456..3172)
                     /locus_tag="NBL02_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HCD2572895.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(3399..3941)
                     /locus_tag="NBL02_002219"
     CDS             complement(3399..3941)
                     /locus_tag="NBL02_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCD2572896.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(3946..4173)
                     /locus_tag="NBL02_002220"
     CDS             complement(3946..4173)
                     /locus_tag="NBL02_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCD2572897.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(4252..6132)
                     /locus_tag="NBL02_002221"
     CDS             complement(4252..6132)
                     /locus_tag="NBL02_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HCD2572898.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(6321..6935)
                     /locus_tag="NBL02_002222"
     CDS             complement(6321..6935)
                     /locus_tag="NBL02_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD2572899.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            7077..7637
                     /locus_tag="NBL02_002223"
     CDS             7077..7637
                     /locus_tag="NBL02_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCD2572900.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
BASE COUNT         2378 a         1550 c         1287 g         2608 t
ORIGIN      
        1 aagaaaaggt ccgctataag cttcctcaag tatttatcag cttgattgtt ttattcggtt
       61 tgattatttt gatgacacaa atttcactca tcatcagagg aaatacagat acagcgctga
      121 ggttttctaa accatattct ctcttcgcaa tgtattatac tttctcttcg tttttatttt
      181 tagaaatgct atctgataaa ttaggtataa aaggaagagg aaattttctt gctgtatttt
      241 tgattgtcac tcaagttata tgtaatttcc ctttagtttc ttatcgcgta tctacattta
      301 tgaaacgtga atttccaaac tcagggaagg catggctgat aaatatcggg cagtttttta
      361 tttgatattt attagcagtg tccctcctag ttatcttttt attttatata caaaaaatcc
      421 gtttcattca tcgtttcatc gaatacttcg cttttgattc gattgaacaa ttaactcagc
      481 gttggaaaaa aagaattcat tggctttttg ttcataaaag ttatttttta gttgcagcat
      541 ttttttactg ctttaccttc gtgcagattt tcgttttaga accaaaagaa ggttggaaag
      601 aaactatcca ggttgcctta aaaatattta ctcagcgaca agcacccatg atcctgacaa
      661 tcatcattat tatgggattt ttcttcttac tattgttgat ttccaatcgt ttttggtacg
      721 ccttgactgc taccttgatc attaacttac tgctcactat ttcgacagta atcaaaatgg
      781 aaatgcggga agagcctgtc tttccttcag atttaaaaat gctgaccgga ttgtcagaac
      841 tattatcgat ggttagccca gtcttattaa tcgtgggcgg attgatcctt ctcctattat
      901 tgattactag tattattgta caacgtagac tacaaaaaca gtattccttg aagattcatt
      961 ggaaacgtcg atttatcagt atcgctgtat tattaggatg tttttcgggt gtctttttca
     1021 ttaatcacaa aaattcgcct tctttcttgc tgtttaattt atttaaagta aacaagactt
     1081 tctttaacca gcaaggcgcc gtaaaagata acggaccagt cattcaattc ctaaacaata
     1141 tcgacattaa aatcatggaa gaacctgctg gctattcgaa agaaaaaatt aatggaataa
     1201 tgaaaaagta caacacaaag gcagatgaaa tcaaccaaac aagaagtgac tggttaaaca
     1261 atcagacgat cgttctcaat ttaagtgaaa gcttcagtga tcctgcacgt gtccctaatc
     1321 tgacagtacc tacagaccct attccttaca tcaaaagcat tataacagaa ggaacaggag
     1381 gattgatgtt atccgtcggt tatggtggcg gaacagctaa catggaatgg gaaggactga
     1441 caggcttgag tatcagtaac ctgtctgctt cgttagttac accttatacc cagctagtgg
     1501 agcgtcagaa aataagtcca aatattaccg atctattcga tgaaaaaata gccatccatc
     1561 cttttacagc tgctctttat cgcagaaaac aagtgtttga taaatttggt tttgacaggt
     1621 tctattattt agacagtccg gataaattaa cttatacgga taaaatagaa caaagcccac
     1681 gcatatcgga tgattcagca tacaaagaaa ccttgaaagc tttacaaagc aatacggaaa
     1741 cgtcacaatt catccaatta tccactatgc aaaaccatat gccttatgat gattactata
     1801 gtgaattgaa ctacactgct gaaggaactg ctgtcattcc taatagaaga aaagaattac
     1861 aaacttatat gcaaggcctt cactataccg atacagcagt aaaaaaattc atccaagaaa
     1921 tcgacaaaat cgataaaccg attacctttg ttttttatgg tgaccattta cccgcaatct
     1981 atagtgggaa tgattcgaaa aaatatggat tagaacagca ccagacagat tatttcattt
     2041 ataacaacaa gtatagtcgt gaacaagcag ctacgttaaa taaagaagtt gttgctccta
     2101 ataactttgg tgctatggct cttgagcatg cgaatatcaa agtaactcct tactatgcgt
     2161 tgttgacaga agtatctaat catctacctg cattaacaat tgatcctaca gaaagcttgt
     2221 ctaatcgttt taatggaaaa caggtctttg ttacagagga tagtcaaatt atccaagaaa
     2281 aaaatttaac tgagaaacaa aaagaaattc tagaagatta tcgtctgata caatatgatc
     2341 ttgttgcagg taaccagtac agtgcaacat gggcgacaca aaaaataaaa gactaacaaa
     2401 taaactgagc aaggggttgt gataaaagac atgtcacaac cccttgttta tctcattaat
     2461 ttgcttgtcc ataaacgatt tgattaaagc catttttcaa ttcattattc gctgtctcgt
     2521 tgaagtaaag ataattatcg ggattcccct gattttgttg tgtcaccaat acttttggct
     2581 gattttgctg aaacccgaat agatagacgt gctttttcag atatggttct gtctctgcat
     2641 cggaatagac ggctgtgatt tgatagtctg cttgacctgt cccactttct gaccattctg
     2701 cagaaacagg cactccacca atagccatct tccactcccc gttcaaaata gcttgaggga
     2761 ctttaagacc atataagtcc acactcattt gattcgtata acttttgtat tgttgtccca
     2821 ttgtttggcc ccatttggtc ataaatgcct ctaattgtgc ggatttgtct gcattccaca
     2881 gtgtttgttc aacttgttcg ctgctttctg aagaggaaga tgaagcctct acagaagaac
     2941 ttgaagtaga tgtttgagtt gtttcagtcg ttttggaaga ctctgacgaa gtagaggtat
     3001 ctgctgtttc tgtacttgct gcagttgact tgctctctgt gctagttgtt gtagtagttg
     3061 caactgttgc tttcgctgtt tcttttgttg attctttttt cgattcattc ccgttggtac
     3121 aaccagctaa caaaaaagct agaccaattg ttactaaaat cctttttttc aattaaaaaa
     3181 ctcctttcga ttactcccac ttctttattc tatcaaataa gcaatgtgat tttattgatt
     3241 tttcgtattt tctcatcaat ctttcaaatc ttctaaaaac tgagaagcac ccgctgatgt
     3301 tgtctgtaat acatcagcgg gtgcttcttt tattttaatc ttcgtgattt tccaatttac
     3361 ctcacgcttt atatttgtat ttgaagaaac atagactttt agtcatcatc gtcttcctca
     3421 tccaaatctt gtcggacagt ttttccttgg agtctttgta cttggtagat gatttcttta
     3481 ttgaaactta aaagttcaac aagcaattgt tgcaagccat atttttcttc tttttcagct
     3541 aatttgattg cccgtgtgat aaagacattt tggaaatcaa aatcttttgc aaagttttcc
     3601 aacattgttg aggctggtaa atatttgttt tgaccatgtt cgtaaatttt tccataacgc
     3661 atgacttctt ctaaagtaga cggaacgatt tcattttcaa tcactaactc atgagagatt
     3721 ttattgaacc atctcgcttc ttgatcagca tattctgaaa aaagggtact taattcttgc
     3781 gaagatgtac ccaaaacata caattgtgct tggatcaatt tcatttcttc taatttaaaa
     3841 tttgctaata catgccccac catggcgcca gcagttggtt tatgatggtc gatatctgct
     3901 tgttttgttt cttgaacaaa tttttcttcc actatattca ttgatttacg cctcttttac
     3961 ttttgctgat tggacttcat aacctaattt ttccacaact gcagctaatt gttcagggtg
     4021 gacttcatct ggatcaaagt ttgctttgat tttgctcgca ttaaacaaaa ctttcgtatt
     4081 gcttacccca ggctgttgag aaaccgcatg ttcgattttt tgcatacagc ttggacaagc
     4141 tagtgtatct aattgcataa taattttttt catcgtagtt cttccttcct ttttcttgat
     4201 agtcttattc tagattacag cttccataaa aaaattgatt cttatcaagt tttacgattt
     4261 ttcttctttg ctttcgtgtc caaaatacaa taaacgcatc gcgttaaaga taacgatcaa
     4321 gatactagct tcgtgaacga acatcccact agccatgtaa ataaacccgg caaataagcc
     4381 gttgagtaaa aataagacga caccaatggc aatcactaca ttttcacggg tatttaaaac
     4441 agttttctta gccaagcggt aggcatgaac caacgattca aaattagatt gcatcaagac
     4501 aacgtcactc gtttcgatag caacatccgt tcctgatccc atagcaatcc cgatatcagc
     4561 cgcagccaaa gagggagaat cattgattcc atctccaata aaagcaacgc gcaatccttg
     4621 ttcttggtat tttttcacaa attgaacctt ttcgtctggt aataattcag catggacttc
     4681 atcaatgcct aacatatcag ctacataatc agcagtcatc tgattatcac ctgtcagcat
     4741 cactaactgt tttgctcctg cttgtttcaa cttagctaat tgttcagcga cttctggtcg
     4801 aatcacatct gacacaccta tgatttgagt gatttttttg tcgattgcta caataatgac
     4861 cgagctgcct gtttgttgta attgatgtaa atcttgtact tgctttggtg tgagagaaag
     4921 tttgtgagcg gcaatcaatt tggtgttgcc cgcacaaact gtatgaccat caatctcagc
     4981 tgtgattcct tgtcccttga ttgtctgatt atctttgact gctaattgtt gaaaatcaat
     5041 gtctttctcc tgagcataat ggatcacggc gcgccccaga ggatgatcgg ataacgtttc
     5101 taatcgagcc actaaagcta agctgtcccc ttcatcctct gtatagttct tgatagtgga
     5161 tacagcagta gttcctttgg tcaatgtacc agttttatca aagacaaaag tatcgatttt
     5221 ggaaaaagta tccattgctt caccgccttt gactaagatg ccccgtttcg ctccattacc
     5281 gattcctgct acattcgaaa caggcgcacc aatgactaaa gcgccaggac aaccaagaac
     5341 caaaacagtg attgctaaac gaaaatcacg actaatcaga ccaacaacca acgcaataag
     5401 taaaacggtt ggagtataat actgagaaaa tcgatcaatg aatttttccg catgggattt
     5461 ggtatcttgt gcttcttcta ctaattcgat aattttggca aaggtcgtat catctccgac
     5521 cttagttgct tcaatcgtca cgtagccatc tgctaaaatc gttccagaga agacttcatc
     5581 tccactgatt ttgacgactt gtcgtgattc accagtcact gcggcttcat ccgcataccc
     5641 tttcccgtca atgactcgtc cgtccacagg aatagctcca ccagttttta ctaagacgat
     5701 atcgccttct tccacgtcat cgatttccac ttcttcagtc gttccatctt cttggacaac
     5761 actagcagta gatggggcca tctcggtcaa agccttgatt gactcccgcg ttttggtcaa
     5821 tgttttttgt tccagatagc taccaaacaa aaacaagaat gtaacgatag ctgactcctt
     5881 gtattctccg atcataaagg caccaagcac agcaatagta acgagtaaat caattgagat
     5941 aactttgact ttcactgcct gatacgcatg gatcataata ggtatgaccg caataataga
     6001 agctatggct agagcaatgt tatagaacaa gacaaaacca gtaaattttc cgataaaacc
     6061 aaaaataatc aacacagctg ataaggcggt gatatgattt cgatgctctt ggatccactt
     6121 ttgtaatttc atctttctcc ctcttttcct ttcattctta cgagactaag tatagatact
     6181 tatccatcat aaaaaattga ccactatcaa gtttagtcaa aaaggcggtt ttcttttggt
     6241 agaatacact acaaaaaata gttagcaaga agactcacta aaagtgatct tgccaactat
     6301 tgtcgtttct atcgaaattt tcattgaatg attttcctgt acagcataca tcggagatct
     6361 atttctttat ctaagatttt tatcttgcta attttttcct ttagtctttc aaatccacgt
     6421 cgttcataga attgatacgt acagccacta tctgtaaaaa ggaatatttc ttttcctttc
     6481 tctctttttt caaattctgt tagcaacttg gtaccgatgc cttttgtttt caaatcagga
     6541 tttgcagcta aaaaaatgat ctctccatct ggcgaatgct tctttaaata tgctgcaagc
     6601 atttgttcat ttgctgtctc gtaagctcct ttactttctt tagcaaatat attttgtaaa
     6661 aaatcgaata tcttaacata tatttgctgt ccaaaagaac gatgtttctt tttttctcct
     6721 tttatctccg ccagcaaaac acctgccaac tcgtcctctg catacaaagc aagaatttga
     6781 gttgctcgat tcaactccat atacaaaaaa tatcttccgt aaagattcag cagaagttca
     6841 ttttccatat atctctcaaa gttcattcct tcaacagcaa aatagatagc ttttttgtaa
     6901 tctttctttt ctaagggttt aaaagttagt ttcatcattt cgcctcctca acttcaatga
     6961 ttgtgttata cttacattat aatagacaca gtgtctacta tctataatca atcatctata
     7021 agtttgtcga ttaagcgaca gctttgcttt gatatgtcgg ataaggagat aggtaaatga
     7081 aaaagacaga tagacgcgtc aaaaaaacag aaaaagcttt ggctgaaaca ctatccacat
     7141 tattagtaaa taaaaagatt caagcaatca ctatacgcga actaacagaa acggcagatg
     7201 tacatcgttc gactttttat acacattata aggatatcta tgatctatat gatcaattag
     7261 aaagcaactt ttttcgggat ctaaatgaaa ttctctctta tgatccagcc catagctatg
     7321 aggaactata cactcgcttg attgactatg tttacgcaaa tactttttta tatcgcttat
     7381 ttatcctcaa ccatggtgat atttcgtttc agaagaaagt taaacaagtt attgaagaaa
     7441 actatctgaa catatggcta tatgaagaca agaaaacgat tatcacagaa gaaatgagat
     7501 tctttactac gtaccacgtc caaggttgtc tatccgttct caatcggtgg gtagaaagca
     7561 attacaagta ctccaaaaaa gaattattag aactcttaca gaaactgaat gatcacatag
     7621 agaaaatcat gccttgaata aaaacatact aagcttagtt aagaagaacc gtaaccttct
     7681 cgtataacaa ctgaatctta tttagccaaa ttgtttactg aagatttttt gagtaaaatt
     7741 tctataattc ctacttattg aaaagtagtt tgtagtgctg cttctccctc tctaagtgat
     7801 atatcttgtt gtaccaatca aat
//
LOCUS       DAHHZJ010000131         7750 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.133, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000131 DAHHZJ010000000
VERSION     DAHHZJ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.133"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            147..1913
                     /locus_tag="NBL02_002224"
     CDS             147..1913
                     /locus_tag="NBL02_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HCD2572901.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2015..3274
                     /locus_tag="NBL02_002225"
     CDS             2015..3274
                     /locus_tag="NBL02_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCD2572902.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3421..4722
                     /locus_tag="NBL02_002226"
     CDS             3421..4722
                     /locus_tag="NBL02_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCD2572903.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4725..5576
                     /locus_tag="NBL02_002227"
     CDS             4725..5576
                     /locus_tag="NBL02_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCD2572904.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5589..6404
                     /locus_tag="NBL02_002228"
     CDS             5589..6404
                     /locus_tag="NBL02_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HCD2572905.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6555..7721
                     /locus_tag="NBL02_002229"
     CDS             6555..7721
                     /locus_tag="NBL02_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCD2572906.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
BASE COUNT         2520 a         1346 c         1557 g         2327 t
ORIGIN      
        1 agttttaagt ctatctattt ggcaagttta aaaaataaaa attcggtatc aaaaaactgt
       61 taccgataac atttttgaaa gcgttgtctt ttgcttctct atcacttata gtgatgacgt
      121 catattaaaa cacaaggagg aaatcaatgg atacagctgc aatttatcat cgaccggaaa
      181 gtgaatatgc atttctatat acgaatacaa gatttcgtat tcggcttcgt actaggaaaa
      241 atgatatcaa aaaagtatat gtgctatgtg gagatccata taccataaca actgaaaaat
      301 ggtatcaaaa acaacgacca atgaaaaaat gtctaagtac caatgttcat gattattggg
      361 aaattgaagt gacgagcgaa acaagtcgct tacagtatgc gttccatgtt gttggggaag
      421 ataacacgga ctgtttttac ggtgatcaag gaatttttcc ttatcgagaa aatgtgatta
      481 ctgaaccaaa tttttatttt cgaatacctt attttcatca aatcgatcga ttcactattc
      541 cagaatgggt aaaagaaaca gtatggtatc aaatttttcc cgaaagattt gcaaacgggg
      601 acaaagcaaa tgaccccgaa cacactttac cttggggaag taaagatcca gaccgagaag
      661 actttttcgg gggagatttg caaggtgtga tggatcattt ggattacttg acggaccttg
      721 gcatcaatgg catctatttt tgtcccatat ttaaagccac atccaatcat aaatacgata
      781 cgattgatta ttatgaggtg gatcccgctt ttggcgataa aaaactttta aaaaacttga
      841 ttgatgaagc acataaaaga gggatacgta tcatgttcga tgctgtattc aatcatatgg
      901 gtgtccattc accacagtgg caagacgttc tcaaaaacgg agaaaaatcg atctataaag
      961 actggttcca catccgtttt ttcccagtag attcttatca gatgactgac ttaccagaaa
     1021 ctgctgaaaa tatcccatat gatacttttg cttttactcc attcatgccg aaactaaata
     1081 cagcaaatcc agaagtgcaa aaatatttgc tggatatcgc aacttattgg ataaaagaat
     1141 tcgatattga tggatggcgt cttgatgttg ctaatgaagt cgatcatcat ttttggaaaa
     1201 aattcagaga agcagttaca gaaatcaaac cagacatcta tatccttgga gagatttggc
     1261 attcctctca agcttggctg caaggggatg aattccatgc agtgatgaat tatgcattta
     1321 cagattcgat taaagattat tttgcaaaaa agaaaatcac agcttcacaa atggtcagtg
     1381 gaatgaatca tcagcaaatg ctttacagag atcaggtgaa cgaaggaaca ttcaacttat
     1441 tagattctca tgatactgcc aggatcttga ccctttgcca aggtaacaaa gaattgatga
     1501 agtctgtgat ggcctttatg tttcttcaaa aagggtctcc ttgtatctat tacgggacag
     1561 aaatagggat gactggtgaa gatgacccgg attgccgcaa atgtatgata tgggagaagg
     1621 aggaacaaga tctagagcta ttcggtttta taaaagaact tgtttctcta aggaaacagt
     1681 tgtctaagat tatttctgaa ggttctactc agtggctaat agtaaatgat cgtgaagata
     1741 agctctattt cacacggaaa ctagagggac agatcatcta tgtttacttt aatcagtcaa
     1801 aagaaccttg ggtagtagaa caggaaaatg aggtgattct tagtcaaaat tgtcagttgt
     1861 tagaagacgg gaaagcagag atacgacaat acggattttt gatcatgtgt tgaaaactgt
     1921 taccggtaac aacaagaaag cgtttgcaaa tgaagatttg tgcccattat aataagggtg
     1981 taacagcacg ttgaatacaa aggaggaaat gaagatgaaa aaaagaagtg tgaaacagtt
     2041 gggtttaggg ttaatctctt taggaatctt aggcggatta gcggcttgtg gcaatggtaa
     2101 ctcttcagca gaaggggaag ccgatgaagg aaaaacgtta acagtttccg ttgattctgg
     2161 atataaagat tatataaacg aaataaaaaa cactttcgaa aaagaaaacg atgtgaaaat
     2221 caagctagta gaaaaggaca tgttcgatca gttggaatca ttagcgttag atgggcctgc
     2281 tggaaaggga cctgacgtta tgatggcagc atatgaccgg atcggtgcgt taggacagca
     2341 aggacatctg gcggaagtaa aactgggaaa tgaagcagct tacgatgaaa cagacaaagc
     2401 ccaagtgact tatgatggaa aaatctacgg agaacctgca gtaatcgaga cccttgtttt
     2461 gtactataac aaagatttag tcgatacagc accatcaacg tttaaagatt tggagaatct
     2521 atccaaagac agtcgttttg cttttgagtc agaagcagga aaaaatacag gatttttagc
     2581 taaatggact gacttttatt attcctatgg tttaattgca ggttacggtg gctatgtatt
     2641 tggtgatgat ggcacaaatc cttcagatat cggtttgaat aatgcaggtg ctgtagaagg
     2701 aatctcttat gcaacagatt ggttcaaaaa tgtttggcca aagggaatgc aagacataaa
     2761 aagtgcagga gacttcgcca gccagcaatt catgtccaat aaaactgcag caatcattga
     2821 cggtccatgg caagcgcaaa catacaaaga aaacaatatc aattatggtg tagccaaaat
     2881 tcctactcta aataatggac agccttatca accgtttggc ggaggaaaag gatgggtagt
     2941 cagcaattat gctaaaaaca aagacctctc tcaaaaatgg cttgattatg taacgaatca
     3001 ggaaaatcaa gaaaaattct ttgaaatggt aaatgaaatc cctgcaaacc aacaagctag
     3061 ggaaacagca aaggctaaaa acgatgaatt aacaacagct gtgattgaac aatatgaaac
     3121 agctcaagcg atgccaaaca tcccagaaat gggcgaagtt tggacaggtg cagaaaactt
     3181 gatgttcgat gcagcatcag gttcaaaaac accaaaagaa tccgcagatg aagcagtaaa
     3241 aacaatcagt gaagccatag aacaaaaata ctgattaaaa tgataaccgt ccatttttat
     3301 aaaaaatgga acaggtgctt cagataaaag tggcaagagc gagatggaaa attatcttgc
     3361 tcttgttgcc attatcttaa aagcagtttg cggtttagca agtttagagg gggcattcag
     3421 atgcagcaag ataaaacaag caatatcaac gaaaaaaaaa tcacgctttg ttcacttatt
     3481 ccagggatgg ggcagtttgt aaatggccaa gcttttaaag gtatcgtttt tttcgccata
     3541 ttcgtcctat tcattggtca gatgcttctg ggcggatttc atgcattggt aggattggtt
     3601 actttgggaa gtataccaat ggaagatcat tcattattta ttttgattga aggcactttg
     3661 caattcatca ttacaatcat tttcttgatt ttttacggtt tgaatctttt agacgcttat
     3721 cgagtagcga agatgcgtca agagggcaaa aaaatcaatc ggacgatgaa ggaagtcata
     3781 cataatatag gtgatgaagg ttttccttat ctattgacac ttcccgctta tcttctcatg
     3841 atttttacga tcattttccc agtcctagtt acattgttca ctgcatttac taattatgac
     3901 ttcaatcata ttccaccagc cagtttaatt gattgggtag gggctaaaaa cttttttagt
     3961 atcttctttt taagctcata tcgtaatact ttttttgcag tcttttcgtg gaccgtgatt
     4021 tggactttat gtgcaacaac tttgcaaatc acccttggta ttctcacagc ggttgttgct
     4081 caccagaaat ttattaaatt caaacgtatt ttcggtatca ttttcttatt accatgggcg
     4141 gtcccagctt ttattactat catgagtttt tcgaatatat tcaacgatag cgtaggtgcc
     4201 atcaatactc aagtgattcc tttcataaac cagcttcctt ttgtagatat cagtgcaatc
     4261 tcttggaaaa cggacccgaa ctggacgaaa gtagcaatta tcttagtgca gggctggtta
     4321 ggatttccct atatctatgt gatggttaca ggaatcttac aatcgatttc agaagactta
     4381 tatgaagcag caaaaatcga cggagcaaat gcatggcagc gcttttcaaa catcacgctt
     4441 cctgctgtct tcctagtggc agcgccaaca tttattacac agtatacggg gaatttcaat
     4501 aatttctcca tgatctatct gtttaacaat ggcggtcccg gaagcgtagg cggaggagct
     4561 ggttctactg atattcttat ttcctggatc tataaactta cgactggaac ttctccgcag
     4621 tattctatgg cttcagcaat cacgttgatt atctccgtca tcgttatctg tgtgtcattg
     4681 ctggtattta aaaagacaaa tgcgttcaat atgggggatt aacaatgaaa aaatttcatt
     4741 cgagtgctcg attccaaaaa cgactttctc acttttttac ttactttttt cttttaatac
     4801 tgtcagtcat tatcatctat ccgcttttga tcacagcaag tactgccttc aagtcaggaa
     4861 atattgcagc ttttagttta gatctttcaa gtgactggac tttaggaaac tttactcggc
     4921 tattcagtga aacactttac gggacttggt ataaaaatac attgatcatt tctgtcctaa
     4981 caatgatcgt acaagtgact gtagtaactt tggcgggcta tacttatagt cgctatcgtt
     5041 ttatcggccg caaatcaagt ttgatgttct ttttagtgat tcagatggta ccgactatgg
     5101 cagcattgac tgccttttat gtcatggctt tcttattaga agcgctcgat aagtactggt
     5161 tcttgacaat gatctatata ggcggtggaa ttccaatgaa tacttggctg atgaaaggat
     5221 actttgacac tgtaccaata gatttagatg aatctgctaa acttgatggg gcagggcatt
     5281 tcaggatttt tttccagatt gtattaccgc ttgtcaaacc gatgctcgcc gttcaagcat
     5341 tatgggcttt tatgggacct tttggggatt tcatgttagc taagttcctg cttcgaacac
     5401 ctgaaaatca gactgttgct gtcggcttac aaacatttat ttctgatgtg acaaaccaaa
     5461 gagtctctct ttttgctgct ggggcaattt tgattgcact accgatttgt ctattattct
     5521 tctttttaca aaagaacttt gttaccggcc tgacatctgg cggaacaaaa ggataggagc
     5581 agagtatcat gcaaacattt ccgttaaact atttttccgc attacgtacg ccaactcaag
     5641 tgtttgcggg acgcaaacta ttatcatggc ctaaattttt cttgatattt gtatttttag
     5701 tttccttgat ggtcatgcct gtgactcttt tttatgccaa tcagatccaa gcgattccat
     5761 tagaacaatt tctatccgtt cattcgctga tcgatgaaca aggaacccaa aaattttctg
     5821 aacttgaatt atctgaaact gggttacaag catcgcaaca aacgatagct gtgacccctg
     5881 aaattttggt aggtgtgtct ttatctgaga aacaacaatc cgatcatgga acttttattg
     5941 attttgagaa ggaacaatgg gtgatccaac aaaaggataa atctggtata cgccgctata
     6001 cgatgaatta tagcccatct ttccaaccag attctgtacg tacgcccgaa gatttccaac
     6061 gatttttaga aagagaattt tatgccagta atcgtccaac aatcatttta tcttatagtt
     6121 taagtcttgg tcttgtccta tttgtcatga cgagtctcat tttattcggg gcttcctttt
     6181 tcttgtggat gacaagaaaa agccaacttt catccattca tacattcaaa gagagtgcaa
     6241 atttgatgct gaatgttatg ggaataggtt ccatggcagc agcagttgta gggtttatcc
     6301 actttgactt tatcttgatg ctaagtgtcc aaacaacgat aactgtcttg ctgcttttat
     6361 gggtttttgt caaaacaaga ttcaaagata agcgtgtcga atgacaagtt taagcgattt
     6421 atgcgacaat gaatgtgaaa aaaatgaaaa ataaagtagt ttttgaaaaa tttctgaaaa
     6481 aattaaactt cttagttgat tcttttcttt tttagtatta acgtaatagg gtagtttttt
     6541 taaggaggaa aatcatgtta gagaaaactt tatacaatca attattttca cgttctttct
     6601 cgctccctgt cgaagtaaat tattgggacg gtacaacgaa acaatacgga gatacggaca
     6661 ctcctcccca aatcaaaatc aatatccatg aagaaatccc aatgaaagag attacaagta
     6721 acgcttcttt ggcactagga gaagcctata tggatcatcg aatcgagatc gagggaagta
     6781 tccaagcatt gatcaacgat gcttatcagc aagcaaacag ctttttacga ggaaaagatt
     6841 atctaaaatg gctgccaaag aaagaaaaac atacgaaaca aaaaaacaaa gaagatatcc
     6901 atgcgcatta tgacttaggt aacgactttt ataaactttg gctagatcca actttgactt
     6961 attcttgtgc ttatttcaat acacctgaag atacgttgga acaagcacaa atcaataaag
     7021 tccatcatat cttagacaaa ctctttatca aagaaggcga aacgttgctt gatatcggtt
     7081 gtggctgggg aacattgatg ttcactgcta ccaaagagta taatgtcaag gcaactggga
     7141 tcacactaag tgaagaacaa tacgatttga ttaccaaaag aataaaagaa gaacatttag
     7201 aagataaatg ccgtgtatta ttaatggatt accgcgaact aaaaggcgaa acgtttgatc
     7261 atatcacaag tgtaggtatg ttcgaacatg taggttctga aaatcttgaa ggttatttca
     7321 aagtggtaaa agatttattg aaaccaaaag gaacagcttt gatccatgga atcagccgac
     7381 aacaaggcgg tgcgacaaat gcttggatca ataaatatat tttccctggc ggttatgtcc
     7441 ctggtgttac tgaattagtt gataatatta caaaaaacga tttgcaactg attgatttgg
     7501 aaagcttacg tcgtgactac caattgacat tagaacattg gacaaagaat tttcatcaag
     7561 tccaagatca agtcacgaaa gaaatgggtg attccttcta tcgaatgtgg gatctatatt
     7621 tacaagcttg tgcagcttca tttgaatcaa gtaatatcga tgtgattcaa tatttgcttg
     7681 ttcaacctgg aaacaatgac atcccaatgc atcgtgcata attgaggcaa aagtactgtt
     7741 taaagactat
//
LOCUS       DAHHZJ010000132         7749 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.134, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000132 DAHHZJ010000000
VERSION     DAHHZJ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.134"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(54..2756)
                     /locus_tag="NBL02_002230"
     CDS             complement(54..2756)
                     /locus_tag="NBL02_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCD2572907.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(2769..4058)
                     /locus_tag="NBL02_002231"
     CDS             complement(2769..4058)
                     /locus_tag="NBL02_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HCD2572908.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            4208..5254
                     /locus_tag="NBL02_002232"
     CDS             4208..5254
                     /locus_tag="NBL02_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCD2572909.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            5256..7409
                     /locus_tag="NBL02_002233"
     CDS             5256..7409
                     /locus_tag="NBL02_002233"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HCD2572910.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT         2181 a         1578 c         1413 g         2577 t
ORIGIN      
        1 taaaatttct ccaccatctg aaaccgcaca cacaaatttt gttccgcctg cttctattcc
       61 gccatacatt cgttttcctc ctcgaatccg attgtccgaa tctcataagg ttggattatt
      121 gggacaaccg cttgcttcgt tggttcttca agcagattca atatgacagt ttttccattg
      181 tctttagtta tttccaattt ttctaaatga ctagacatat tgaaacctct tactactact
      241 ttatcactaa acttgctgcg tttcaaagct gtaattgcaa acgtttcact ttttatcgtt
      301 aaatattgtt gttttgaaat caatgttcct tcatgatgtt tgatttgttg agtggaaaaa
      361 ggaatctgtg ctgcgtatgc atgcaaataa gtagagaatt tttcttctgg tccatgtagt
      421 tcaatgctgt aattgaaccg atgctcacct agacactgtg cttctggtgt tggaaaatat
      481 ccccaatcgc ctagttctcc cacactgcga agtagcgtca ctgcgatttg tccgtcttct
      541 gttacttcat attcattcaa accaaagttt ccgactgtta caccgtactc ttcatcatga
      601 atattgacaa atgcttgctg atgttgcgga ttcgtggggt tttcccaact tttagacaca
      661 tggtttgggc gcttcaccac ttcaaaaata ctatctgctt cgtgatgttc cacatgtaac
      721 tttgtaggaa acaagacacg taagcgatga tctttcatct gattgtcgag agtcgtttca
      781 aagtcgattt ttttactatc ttttctgatt gtcatgattg tttttatttg aaggattcgt
      841 ttttctttgg atcgttctgc ttttcgataa cgaaattcca caaccatctg ctgttctttt
      901 tctaaacgct cgtctgcaga aacagggatt tccatttcct gtagaatact tatttttgct
      961 atttccggtg tatctactaa tacttttact tcagagtttt ctttgttact ggataggatt
     1021 ggctgatcac agaaaggttg tttaaagata tactcatttc ctatatcgcc gatatcttca
     1081 aaaatatgca gattattcaa ctttcgtttg tttttgcgat cttccattgt aatggtacca
     1141 tttttttcaa tcgtcaaatg tagagggccg ttttcgatga ttcgtccact ctgatgaatc
     1201 attgtttcct tttcttctgc tttcgcttcc ccttctacaa gcgcgaatgt ttcccaagaa
     1261 aatgctggca tgttttctaa tggtaaagtc actttgatca ttcttgccat atatggcaca
     1321 cggaaacggt ctttcggcaa atcataacca aattggacag tttcttcaga taaaatagct
     1381 ggtatagtgg ccccactttt tgtttctaca tgatacttac gtttaggcaa attttctaac
     1441 tcttgatata gttgttcagg tattccttca gcgaatcttt ttctttctaa ttcgatggtt
     1501 atttctgctt caccagtttt atccattcct gcagtattta caatgacaaa tggaaagctg
     1561 tcttttggga atccggtcgt atcgattgct gcggtcagct gttctaagga atcttgtgca
     1621 agatactttc caacttcatc tgctttttca aaacgaggta tcatttcccg atggacagaa
     1681 tcgactgaac aaccgcaaat actgtcgtga ggatgatttt gcattaatgt cttccaagca
     1741 taatctaatt gatcatgggg atagtttcca ctaacttcat aagccatcgt cgcaagtggt
     1801 tctgtaatat tttctagctg tcgctggacc ttggtattcc attgttttaa gtaaacccga
     1861 gcagatgctg tattggctaa agtataccag ccatccgttt cttgacttgt caattctcct
     1921 tctactgagc ctaagtcttc tggcacatct ttttgaactg cttccagata gtcagtaaaa
     1981 ttagaatgaa taaactcgta atctggaaac agttcatttg cgagatggat tgcttttgat
     2041 atatcttttt gtaccggttg atgatcaaca ccattcatca tcaataagtg gttcgtagag
     2101 gcatattgtt ctgcatccgc taatttttgc ttccaaaagg caagagctgc ttctttttct
     2161 actggaatct cattcccgtt gctataccaa ttggcaaata gcagaccaaa gattgctgtt
     2221 tgatcaggtc ctttccacca catctctgag tattgggaag aatagttttc tgcttccaaa
     2281 acttgattat caaagccaat tggttttaca ccacggccaa acgctgctgc tgaaatacca
     2341 gcttgtttca ttaattgagg cgtctgtccc atatttccaa atgtatcggg aaaatagcct
     2401 aacatgacag gggttcccca tttacggctt tcttccatgc caatcagcat atttctcacg
     2461 ttcgattccg agctgataag aaaatcatct tgcaagatat aaaatggtcc aattctcaat
     2521 tttccttcat tgattgcttg ttggattgcc tgtctttttt ctggccttac ttgaagatag
     2581 tcatctaaaa taatcgtttg cccatccaaa tgaaaactgt tgaaggacgg atcaacttca
     2641 aatagctcta gtagatcgtc tatcaattca atcagtcgca tgtgatgctg ttcgtaagcc
     2701 atgtaccatt cacgatccca gtggctatga gaaatgatat atactttctt tttcatttac
     2761 gatacctcct acttttctac tctaatatca aagtaatcca tgactaattc gcaaaacatc
     2821 atatttgccc aagaaaacca ttctctcgta taattttcag gattgttcac atcgaatcct
     2881 tcgtgcataa ggtgtgttcc tgcgtcagtt gctacaagta aattcaatat ccgctctttt
     2941 tcagatttgt ctgacgttgt cattccttcc atagctaatg caatcggcca aacgtatttt
     3001 tctggcgtat gggaggatcc aattcctttt gcgtactttc cttcatagaa atatggattt
     3061 tctttactca acaatgttct tcttgtttgc agataatctt cctcttgttc agaacaatat
     3121 cccaagtaag gtgcggcgat cagattgggc acattactgt catccatgac tgtggcgttt
     3181 cccattccat ctacttcata tgcataaatc gtttgccctt tttgattaac cgttcgtccg
     3241 tgattctgaa tcccctcttg gatctctttc ttcaatttct gtgtttgctg tacaatttct
     3301 ttatcatcga gtatcgtttg aaaaatttct tccacataac ctaacacgac caccgcaaac
     3361 atattggatg gtaccagata tccataacgg caagtatcat cgctaggacg gaaaccagac
     3421 catgtcattc ctgtaaaagc tgtttttgca cctcgcccgt cattgatcag cgtatcttct
     3481 tttcttgtcg tgtctcgtac aaatgtataa ggagagtgtt catgatcttg ctctgttttg
     3541 aacacgtcca agattttttt gataccttca ataaaagatt gattgaattg atcagttttt
     3601 cctgtatttt tgtaaagaag ataggccaac tgtattgggt agcataaaga atcaatttca
     3661 tattttcttt cccagatcca atcgttcatt tttgtatgat ctgtttggtg gccagcaccg
     3721 ttagcttctt cgttgaaggc attcgcatat ggatcgatat tgatataaaa aaattgcttt
     3781 ttgactaacc cgctgatcat gtcagctaat tcctcatctt cttttgcaat cactaaataa
     3841 ggacgaactt gtgcagtaga gtctcttaac cacatcgctg gaatgtctcc cgtcaataaa
     3901 aaggtggttc cgtcattttg ctttttgact gttgtcaaca gagtattggc aaatgctgct
     3961 gtaaaattct ttgcccagtc cgcatggata tccccacatt tttgtgtgat atcttccata
     4021 aatttctgca ccgattttgg tatatcttta taagccacta ataaaacctt ctttctattt
     4081 gatatatctt taaagattat acttcataca gccaattatg tctacgcttt caaaaaatat
     4141 ctttttattt actttttcat ccattatggt attatttgaa tatgatatat cattaagagg
     4201 tgtttatatg aatcaaccat tgtaccagac aatatttaat gatttgcaaa cggctatttt
     4261 aactggtgaa ttacctgtag acagccaact accaactgaa aaagaacttt cacgtaccta
     4321 taaagtaagc agaatcactt ccaaacgtgc attgactgaa ttagagcaat taggacttat
     4381 ttatcgcgtt cgcgggaaag gcagtttcgt taaatccgct tctccggaac agattcttgc
     4441 ttctcctaca aaaacacgac ggattctgtt tcttcttcca tatgtcgctg atttgtctgt
     4501 tggtgatttt agcaagggat tgaaccctgt gatgcaagat aaacagattg atgtcatgat
     4561 gacgacgctt gactttctac aaaataaaac agcaaaagag ctaatcaatg aatttgacgg
     4621 attgatttat tatgctcttg atacagaaca acatcttgac ttactttttg agctttcatt
     4681 gaaagaattc cctgtcgtca tattagataa aaaaatctat gagttacctt ttcctaccgt
     4741 tctttccgat aatttccaag gcggtgcgct tgctgcctct tatttgatcg aacagggaca
     4801 ttcaaaaatc gcttatttgt ttggtacgca gactcatcca caatccgttc gacagcgtta
     4861 ccttggttac cttgaagcat tggacaaagc tggtttgact ttccacactt cactggatga
     4921 caaaagcgcg acattagatg aattgctttc ttatatcact aagtatgaag taactgcttt
     4981 tgtttgtgaa aacgatgttg tcgctatcca gaccatgaat ctcttgagac agcatggtta
     5041 tcatatccct gaagattttt caattatcgg atttgatgat atccaagcag cagctcttgt
     5101 tgacccgcca ctgacaacca tcgcacaaga ttttgaacag ttaggtaagc ttgcgggaga
     5161 tgctttggtc acttggttag agacaaaaga tcgaccatcc gatagtaaat ttccagtttc
     5221 attcatcaaa agacaatcaa caaaggagaa ataaaatgaa tatcggtcaa atagatacta
     5281 gacatgcaac agcgaatcag ccaaatttct ctaatgggaa ctgccagcct tatactggcg
     5341 ttccttttgg gatgaattat tttgcaccgc aaactacgga tcaaaacggc agctggtggt
     5401 ttcatcctga tgaccgcgtt tttcaaggct accgtcttac ccatcaacca agtccttgga
     5461 tgggtgattt cagtcatatg ctgtttacgc cgatcaatgg aaaactacag gagaacaccc
     5521 ttttccatgc tcaaagttct taccgcccag aagaaagtat attttgcccg actcatctgt
     5581 cgatctgtca attacgttat ggcatctgtt ctacattgat acctagcatg tatggcggaa
     5641 tcttatctat tgattacagc aaaaatgaca gcggtttact tttatccttt ccgggacgcc
     5701 atcagctctt tgttatagat ccttacacag tggaaggaaa aatcacacat tttgctggtt
     5761 cagaagatcc tgatttttct ttttatttta tattgagatt tgaatatcct ttaacaattg
     5821 atgagttagc tgtttccaac aaaaatagcg actcgatccc gttgtatttt gaacagacaa
     5881 aaaaacaaac tatccgattt gggacatctt ttatcagctc ggaacaagca gcttataacc
     5941 tggaactaga acaaaaccaa agcaaagaga cttaccttgc tcacagtcaa gaacaatggc
     6001 agtcctattt tgatcgtata cagctcacac accatgatca acaacaattg cggacgtttt
     6061 atcataattt atatcgggtg tttttatttc cacaaacatt ttatgaaatt gatcctagtg
     6121 ggcaaaaaat ccactatgat acgactagtc gatctataaa acctggtgtt ctctatacaa
     6181 ataatgggtt ttgggatacg tataaaacgg tttatcctct ttattctttg attgctgtag
     6241 aaaaatacga agaaatgctg gaaggttttt tgaacagcta tagagaaagt ggctatttgc
     6301 caaaatggct gtctcctgat gaacgtggac ttatgcctgg gacattgatc gatgcggtca
     6361 ttgcagatgc tgctgtaaaa gatatccgac gagacttgat gcctgaattt ctaacggcaa
     6421 tggaaaaagg tgctacggtt caaagcaaac atccaaatta tggacgccaa ggaacagaag
     6481 attacttgac atatggctat gttcctaatc actaccacga atcagtcaat cacacattag
     6541 actatgctta tagcgatttt tgtattagcc aagttgccaa ggtactagaa gaagaggaaa
     6601 tgacgaagaa atatcagcag caatcattga actatcaaaa tatctttgac tatgagtcag
     6661 gatttatgcg ttcaaaagat aaagatggca agttcagaga accattttca gctaatcgtt
     6721 ggggacaaga ttatgcagaa ggaagtgctt ggcaaagcag ctttgctgtt tatcacgatt
     6781 tttcaggact gatcggaaaa tatggtggca gtgagagata taaagaaaaa ctcattgagc
     6841 tgtgcaatca gcccccaacg tttgacccag gaggatatgg ttttgagatc cacgaaatga
     6901 gtgagatggc cgcgatagaa tttggacaat tagctatttc aaaccagcca agttttcact
     6961 atccttactt gttcagttat ataggacaac ctgaaatggc acagccactg cttaaacagt
     7021 tgatgactca gaccttcgac gattctccaa tgggttaccc tggagatgag gacaatggaa
     7081 gtatggccgc ttggtatatc ttcaatagtc tgggatttta tcctgtcacg cctggaaccg
     7141 gtgaatatgt tatcggtatg ccattaattg acaaggctgt tttgcttttg tcaaatggaa
     7201 aggaattgac gattgaagca acgcctaatc ggccgcagca acaatttatc cataaaatca
     7261 aacggatgga caaagaatat acgaaattat attttaccca tcaagatttg ctgaaaggcg
     7321 gcacgatctc ttaccaatta gggatcatcc ctcaccctaa gaaatatcaa gcaaaagatt
     7381 tgcctttttc cttatcaaac tataattaac aagaggttgt gaaaaagctg tcctgcatca
     7441 agtaataaga acagccaagc aaaaatagat cctcatattt ttcgtttggt tggtcttatt
     7501 accgcagatg caagcttttg aacaccgttt attgagaggt tgtgaaatca gcgttctgac
     7561 ctgagtatta gagagacaaa tggcaaaata gttcctcata ttttgccatt ttttgagcta
     7621 ataccgaggg tcagctgatt gaacaccgtt tagtggagag gtcgtgaaaa agctgtttag
     7681 tttcgagtat taagaaaaat tttgtgaaaa tggctttcca catttttgca taaatttggc
     7741 ttaataccg
//
LOCUS       DAHHZJ010000133         7587 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.135, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000133 DAHHZJ010000000
VERSION     DAHHZJ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.135"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            120..2729
                     /gene="clpB"
                     /locus_tag="NBL02_002234"
     CDS             120..2729
                     /gene="clpB"
                     /locus_tag="NBL02_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCD2572911.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2880..3566)
                     /locus_tag="NBL02_002235"
     CDS             complement(2880..3566)
                     /locus_tag="NBL02_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572912.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3544..4038)
                     /locus_tag="NBL02_002236"
     CDS             complement(3544..4038)
                     /locus_tag="NBL02_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HCD2572913.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4058..5278)
                     /locus_tag="NBL02_002237"
     CDS             complement(4058..5278)
                     /locus_tag="NBL02_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD2572914.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5425..7260
                     /gene="lepA"
                     /locus_tag="NBL02_002238"
     CDS             5425..7260
                     /gene="lepA"
                     /locus_tag="NBL02_002238"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCD2572915.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            7456..>7587
                     /locus_tag="NBL02_002239"
     CDS             7456..>7587
                     /locus_tag="NBL02_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD2572916.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     "
BASE COUNT         2523 a         1423 c         1542 g         2099 t
ORIGIN      
        1 gaaaaaaagt caaaaaacta tgtgtatatt ttgaccaaaa ttgaccaaag cgatataata
       61 gtttttgtaa agatcaaaga aataagtgat cttaccttta attaggaggg tatacctgta
      121 tgaatattga gaaaatgact acgacgcttc aagaagcgat tgctgaagct caacagattg
      181 cggtcactcg aaaacatcaa gatatcgaca ttgcccatgt atggaaaatc tttttacaat
      241 ctaatcattt tggtcgaaat ttctatacgg atgccggatt ggatgtcgaa tcttttgaac
      301 atgaaattga tcgtctactg gatgagtatc cagttgtttc tggtggaaac gtccaatatg
      361 gacaaaacct cagccagaat ttctttcgat tattgaatga agcagaccaa atacgtgaaa
      421 gcttcggcga cgagtttctt tcaacggaag tcgttatttt agcgttgatg aaattaaaaa
      481 attatccgtt aactgtttat ttgaataaaa acggattatc tgaaaaagaa ttgcgtaaaa
      541 acatcgaaga gatgagagga ggagaaagag tgacctcaca aaaccaagaa gaacaataca
      601 aagcattaga aaaatacggc gttgatttag tacagcaagt caaaagcgga aaaatggatc
      661 caatcatcgg tcgtgatgaa gaaatccgtg atgtcatccg tattctttca cgaaaaacaa
      721 aaaataatcc tgttctgata ggagaacccg gtgtagggaa aacggcaatc gttgaagggc
      781 ttgctcaacg tatcgtacgc aaagatgtgc ctgaaaattt aaaagataaa acgatttttt
      841 cattagatat gggtgcattg atcgctggag caaaattccg tggtgaattt gaagaaagac
      901 taaaagcagt cttgaaagaa gtcaaaaaaa gtgatggacg tatcctcttg ttcatcgatg
      961 aaatccacaa tatcgtaggg gcaggaaaaa cagaaggaag tatggatgcc ggaaatctgt
     1021 tgaaaccaat gcttgctcgc ggtgaattac atttgatcgg tgccacaact ttagatgagt
     1081 accgtcaata tatggaaaaa gacaaagcgt tggaacgtcg ctttcaaaag gtattagtca
     1141 aagaaccaac agtggaagat acgatcagta ttctgcgtgg attaaaagaa cgttttgaga
     1201 tccaccatgg tgttaacatc catgacaacg cattagtagc agcagctact ttatctgacc
     1261 gttatattac tgatcggttc ttaccggaca aagcaatcga tttgatcgat gaagccagtg
     1321 caacgattcg ggtagaaatg aactctatgc caacggaatt agatcaagtt actagacgtt
     1381 tgatgcagtt ggaaatcgaa gaagctgcac tgaaaaaaga atcagatgat gcaagtaaaa
     1441 aacgcttgaa aaatttgcaa gaagaattag ctgaacttcg tgaagaagca aatgcgatga
     1501 agatgcagtg ggaaacagaa aaagaagaag tcaattctgt ttcagcaaaa cgtgctgaaa
     1561 tcgataaagc aaaacacgaa ttagaagatg cagaaaataa ctatgattta gaacgcgctg
     1621 ctgtattgcg tcatggtacg atacctcaat tagaaaaaga attgaaggaa ttagaagcta
     1681 aagcaaaaga cagcgagatt aagatggttc aagagtccgt taccgaaaat gagattgctc
     1741 aagtcgttgg cagattaacg ggtatccccg taacgaaact tgtagaaggt gaacgagaaa
     1801 aactaattaa attgaatgaa acacttcata aacgagtgat tggtcaagat gaagcagttg
     1861 atgcggtcag tgacgcggta attcgttcac gggctggatt gcaagaccca aatcgtcctc
     1921 taggttcttt ccttttccta ggaccaactg gtgtcggaaa aaccgaatta gcgaaagcgt
     1981 tagctgagaa cttgtttgac tcagaagatc atatggtacg gatcgatatg agtgaatata
     2041 tggaaaaaca tgctgtgtct cgtctagtcg gagctcctcc aggctatgta ggttacgaag
     2101 aaggtggtca attaacggaa gctgtcagac gaaatcctta tacaatcgtc ttgttggatg
     2161 aaatcgaaaa agcgcatccc gatgtcttca atatcttgtt acaagtccta gatgacggac
     2221 gcttgactga ttcaaaaggg cgagtagtag atttcaaaaa tacggttctg atcatgacca
     2281 gcaatatcgg ttcacagtta ctgctggaag gcgtgactgc tgatggaacg attccagaag
     2341 cagtggctga acaagtcaac acgctattac gtggaaactt caaaccagaa ttcttgaacc
     2401 gaatcgacga cacgatttta tttacaccat tgagcctaga caacgtgaaa ggaatcgttg
     2461 acaaaatggt ggcgcaattg gcacaacgtc tggaacatca agaaatcctg ctgacaatca
     2521 gtgatgaagc caaaacgtgg attgctgaaa atgcttatga accagcatat ggtgcaagac
     2581 cattgaaacg atttatcact aaggaagtag aaacaccact agctaaagaa atcgttgcag
     2641 gacatgttat gcctaaatca aaagtaacga ttactttact tgatggacaa cttcatttca
     2701 aaacagaaga gctagaagaa atcgtctaat cttccattga gactataaaa gaaaaaaggg
     2761 gtctgacaca actagtgtca gacctccttt tttcaaataa acagtgttcc agaagtagtt
     2821 tcttcgaaaa ataagccgaa atttcttgaa gctgcccgct tctgtcacaa cctcttttat
     2881 taggtatttt tcactttttc taatttataa ctttcaaagt aacgctcatc ttttttactt
     2941 tgagaccatg caaacaatcc cattaaggca gcacctatcg tatttgtcag caagtcgccc
     3001 atcgtatcca tcaatgctgc tcgtccgaca aacagtgtgc catcagaagc agcaaaacgt
     3061 tgaagattca tgcctaagaa ctggtcgcat aagaattccc agaactccca aaacacgccg
     3121 caaagtccag caaacgcaaa gccgaataag aggaacaacc aaggactggt ttttgatatc
     3181 tcagcatctt tcatcaagta accaatcaat ccatagccaa gagctgtcag gaccattgga
     3241 ctcaccgcat gtaagatttt gtcccaaaaa gagatgatac tgatcaaatg catccctgtg
     3301 cctaaaaaga cagagatgaa caagaaaaac cagtaaaaca aaacgattgc tgctggtatt
     3361 ttaagtttga aaactttcaa aatgacttgt ggcatgaaaa tcaatgcaat ccctaataaa
     3421 gattccacga taaatgtgat tccttgagaa gtcgaatatt tttgtgtgat aaattcataa
     3481 aaattatagc agacaacgaa tacaccaaaa gcaagtaaag ctatttgcac ctttttgatt
     3541 tctttaatca ctgaaagtcc ctccatctaa aaatcttttt acttctgaaa gcaactcaat
     3601 ttctgaagaa accacttttc cgttcaattt gatcaaacct attgtatata agttcaggta
     3661 gtgaaattga ttttcagctg tttcttgtag tgcagcgatt tttttcgggt gggttccgcc
     3721 ttgctgtcga gtatcagtat ataagccgat cactgggatg cctttcgcat aagccacacc
     3781 gatttccgat gctaccccgg catcgatcgt cagcccatct agtaaagcaa tcattaaatc
     3841 actagccaac actttttctg tgtcagcaag agcaatcatt cgactgtctg cataagcagt
     3901 tttgtcattg attgcttcat tttcttgagg aagatagatc gaaagctcag aagagagctc
     3961 tctgatttct ttaactaact gttggttata taacaaatca cttttagcaa acattggtgc
     4021 ggcaaaataa attttcatct aaacatcatc ctttttatca aaaataatcg ctaaagtaag
     4081 cttccatgtt tggaatgata cgctctagtg acttcagtgt ttcttcatct gtttctaacc
     4141 gttcgattct tgccaaataa gaagcacggc gataattcat catcaagcaa gtcaatgttt
     4201 ggatatccag tcgaacggat tttcctatag gttcatttaa gacacggact tgtccctgtt
     4261 catcccatgt taaagcgaag acaccattat tccaaggagc gacaggatct tcaatgatga
     4321 aatgaaaagc atcagctgtt ccaacaaacg gaaaccgttg aaggaattct tttacatcaa
     4381 cgatccgagc cataaaatat ggttcgatct gctctttgat ctgactatct tcaatcaaaa
     4441 atgctaaagg ctcattttta taaatatctc catgtaccca gtagatcatg gaaaaatgtg
     4501 cggaaataaa gttccataag ccatttcgag cttcttgatc aagatagaac atttctttta
     4561 cgtgaaatat ttcttcagct acccagtaga acagaacacc tcttggttca tggttgctat
     4621 cgtagtagac agcggctgtg cgctcatctt cattttcgaa acgccaatac tcttcccaat
     4681 gaaaatcact gcggaataaa gcaccatggt tttgttgggc aaatttgtca taaacggaaa
     4741 atacgtctgg atgatcaact ggtaatcgct caaccatacc acttactggg atttgtttag
     4801 gtagctgtgt atctctgatt ttaaaggaaa gtttatcaga catgatttcc catcctttac
     4861 gccgatagta ggggatactg taaggataca aataagagat ccattgctta ttcttgcgca
     4921 tattatccaa tgcaacgata attagatctt gcatcaatcc atggttagca tattctggat
     4981 aagttccaac tccagtcaca ccacccattt tataacgtgc accgtgaata ttgacttcac
     5041 aaggatagat agcgatttga gagatcagtt tattttcgtg gaaccatccg aacactttcg
     5101 atacttccaa aatcggtttt ttggaacgga taaaagctcg tttattctca aacccgcttt
     5161 cctcgatgtc tgcttcagtc acttggaaca cataggaaag caattcattg aattgatcga
     5221 tatgttcttg agtgaccggt tttaattcaa gattttctct aaaatctttt tgatccattt
     5281 tttcatctcc atttattagc ttgataacag tatagcaaaa ttctacaaaa aaaacttcta
     5341 tagctgacta attaaaaact ctttaaatta ctgcttttcg ttggtataat ggaaagtgct
     5401 gattatatga aaagaggaga cgctatggat ataaagaaaa tgaaagaacg ccaagagcag
     5461 attcgtaatt tttcgatcat cgctcatatc gaccatggga aatctacatt ggccgatcgt
     5521 attttagaaa tgacgcacac tgtgacgtct cgcgaaatgc aagatcagct gttagactcg
     5581 atggatttgg aacgtgaacg tggaatcacg attaaattaa atgctgtaga actcaattac
     5641 acagcgaaaa atggagaaac atatatcttt cacttgattg acaccccagg gcacgtcgac
     5701 ttcacctatg aagtctctcg aagccttgct gcctgtgagg gtgccgtttt agtggtcgat
     5761 gcagcacaag gaatcgaagc acaaacattg gccaatgttt atttagcgtt agacaatgat
     5821 ttggagatcc tgccagtcat caataaaatc gacctacccg cagcagatcc cgaacgtgtc
     5881 cgtcaagaaa tcgaagacgt aatcggaatc gatgctagtg aagctgtatt agccagtgcc
     5941 aaatcaggga ttgggattga agatattctc gagcagatcg tggaatatgt tcctgcacca
     6001 agtggagact tagatgcacc gctaaaagct ttgatctttg actctattta tgatagttac
     6061 cgcggtgttg ttctaaatgt ccggatcacg gacggtatgg tcaaacccgg agacaaaatc
     6121 aaactgatga gtaatggcaa aacatttgat gttacagagg tcggggtctt ctcgccaaaa
     6181 gcagttgcta gagacttttt gatggtcggt gatgtcggtt atatcacagc aagtatcaaa
     6241 actgtccaag atacacgagt aggggataca gtaacgttag cagacaatcc tgccgctgaa
     6301 gctttaccag gttaccgaaa aatgaatccg atggtttatt gtggtcttta tccaattgat
     6361 acttctcgtt acaacgattt gcgggaagct ttagaaaaac tgcaattgaa tgatgctgct
     6421 ttacaattcg aaccagaaac ctctcaagca ttaggttttg ggttccgctg tggattcctc
     6481 ggacttcttc atatggatgt cgtccaagaa cgcttggaac gggaattcaa tctagaattg
     6541 atcacgactg caccatccgt tatttaccat gttaataaaa cggatggtac gactgctaca
     6601 gtagataacc cggcagattt tcctgaacct gtaacgattc aggatgtaga agaaccattc
     6661 gtcaaagcac agatcatggt cccaaatgat tttgtcggtg cagtaatgga actttctcaa
     6721 cgaaaacggg gagaattcat tacgatggat tatttagatg attaccgagt aaatgtcgtc
     6781 tataatattc cgctttctga aattgtcttt gactttttcg acaaattgaa atcaagcacg
     6841 aaaggctatg cctcattgga ttatgaaatg tctggttatc aaaaaagcaa gttagtgaag
     6901 atggacatct tgctgaatgg agaaaaagtc gatgcattaa gctttatcgt tcaccgagac
     6961 tttgcctatg aacgtggaaa agcaatcgtt gaaaaactga aaaaactaat cccacgccaa
     7021 caatttgagg tgccaatcca agcagctatt ggacaaaaaa tcgttgcccg ttcagacatc
     7081 aaagccttgc gtaaaaacgt cttggctaaa tgttatggtg gagatgtttc tcgtaaacgt
     7141 aaactcttag agaaacaaaa agaagggaag aagcggatga aacaaatcgg ctccgtcgaa
     7201 gttccgcaag aagccttcat ggccgttctg aaaatggatg agcaagatag tgtgaaataa
     7261 cttataatca atcgaaaaac ctattaaata aacctttttg agtagatgca acactgagga
     7321 ttatctgtat aaaagatttt aaactgtcat ttggttatca cggcgatact aaaattaaca
     7381 aaattggaat atttttcttg aagattttta gctgtcagta tatgatgaaa gcatctcata
     7441 ggaggtgctt ttttcatggt tgataattca agtggaaggg ttcttaaatc tctaagaaaa
     7501 gagaaaaaac tatctcaaaa aaagttagct gatttagcag gtatttcaca aagtacttta
     7561 gttaaatatg aaaaagggtc aagaaaa
//
LOCUS       DAHHZJ010000134         7586 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.136, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000134 DAHHZJ010000000
VERSION     DAHHZJ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.136"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            39..1466
                     /locus_tag="NBL02_002240"
     CDS             39..1466
                     /locus_tag="NBL02_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD2572917.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1558..1923)
                     /locus_tag="NBL02_002241"
     CDS             complement(1558..1923)
                     /locus_tag="NBL02_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HCD2572918.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1920..2285)
                     /locus_tag="NBL02_002242"
     CDS             complement(1920..2285)
                     /locus_tag="NBL02_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HCD2572919.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2477..2725)
                     /locus_tag="NBL02_002243"
     CDS             complement(2477..2725)
                     /locus_tag="NBL02_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572920.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2724..2852
                     /locus_tag="NBL02_002244"
     CDS             2724..2852
                     /locus_tag="NBL02_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HCD2572921.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2909..4603
                     /locus_tag="NBL02_002245"
     CDS             2909..4603
                     /locus_tag="NBL02_002245"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HCD2572922.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4607..4726
                     /locus_tag="NBL02_002246"
                     /pseudo
     CDS             4607..4726
                     /locus_tag="NBL02_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            4703..4876
                     /locus_tag="NBL02_002247"
     CDS             4703..4876
                     /locus_tag="NBL02_002247"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572923.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5254..6387
                     /locus_tag="NBL02_002248"
     CDS             5254..6387
                     /locus_tag="NBL02_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCD2572924.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6418..6738
                     /locus_tag="NBL02_002249"
     CDS             6418..6738
                     /locus_tag="NBL02_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HCD2572925.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6767..7231)
                     /locus_tag="NBL02_002250"
     CDS             complement(6767..7231)
                     /locus_tag="NBL02_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD2572926.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7363..>7586)
                     /locus_tag="NBL02_002251"
     CDS             complement(7363..>7586)
                     /locus_tag="NBL02_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCD2572927.1"
                     /translation="VSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAA
                     VNLVLSILIYLPFVKVATIQERRKEALDA"
BASE COUNT         2479 a         1464 c         1369 g         2274 t
ORIGIN      
        1 ccatattgtg caaaaaaata caaggcggag gctctactat gattgaagaa tatattgaga
       61 aaaatatttt aagacagctg tttttatgtg ggcaattcta tgtaaataaa gaagtcaatc
      121 tagaaaagct ctccaatctc ctgcatgttt gcaaaaccac tttactcaat gatatcaata
      181 acataaaaaa agaatttgaa gaacagatcg cttatacaca tcgggaaaag gaccgttaca
      241 ctttatattt ttcagaacat atacctcgat gcaagatcat gcagcaattg tctcaaaatt
      301 cgttgttttt aaaaacttgt ctgctttatt tggaagaaga tgaacctgat tatcttcaat
      361 taactgaatg tgaattcatc tctgtcagca aagcttattc attaaaaaaa caagtattag
      421 cttattttaa tgactgcggg atcgaaatcg accgctactc ccctcgattc actgaaatgg
      481 aaagacgtct gctgcttctt aatgtgtcgt atcgtttagg cggttttaat tcttgggagc
      541 tacctgaatc gttttttgaa cgtgccgatc gtttcattga atccgtaacg gaaaatagtg
      601 gcagatttta tgataaagaa aataaagaaa tcttatcgat tggttttgct atcagtttcc
      661 tacgccagca agtgtgtgct gtgacaatag attcaaaatt tatcgaagaa atcaaaaaac
      721 gtcctattta caattatgtc gaatcagcat gggaaaacac tgatttccaa acctattata
      781 aaaaagaaga attcgccttt attttgacac tattcaatct ttgtaattat ggtttccatt
      841 cttatcagtt gattgcggaa gactttcaac agctacacca agtgtttatt gacaatactc
      901 ccgaaataaa agaactagtt gctacctttg aatctcactt caatcaagaa ttattcggaa
      961 accaaccgtt cgaacgtgcg ctgattcatc tgatgcgttc ggcttgggat aactatcaat
     1021 tattcatgcc agaaaaattt tatcttttaa atgaggaaca gacaaattta tataaagaag
     1081 tccagacaat tttttccagc tggtccagcc agttacctta tgatctacgg ttgaatccta
     1141 attgcatgcg ggcttttgtg atcgaattga gcggaattct ccgtctgaca aaagaacatc
     1201 tgactatcta tatcgtcaca aattcggatg ttcattatct gatttatcgg gaagccttgg
     1261 aagctgtaac tactttcgat tttcaagttg caccaacaat ttattcaagt atatctgata
     1321 taaagaagta tgcgcagcag tcctcaaacc gggtattatg tgaacgtaca ttatatacac
     1381 cagatgcggt ccaatatgaa aatattattc ctatttcgat caatacgatc gatcgagcta
     1441 tcatttcagc tgtgcaaaac aaataagggg ctgtgacaaa aatcatgtca cagcctctta
     1501 tttgttgaag atgcccattt ctgtaacaat ctcttgatac acgtcaggct cacaacctca
     1561 cttattttgg acatctacgg gtccgatctc tttatttaaa aaataagaaa tgatggttcg
     1621 gataactacc aacgccgcta gttggaaaat atcctgcaag gtaggtttga taatcgattc
     1681 gatgatccca gctgaaacaa gaatttccaa acttagaagt atatagcctc ccaacatctt
     1741 tttaatagca ttgttctcgc tgactcggtc gctttgcact tttgtatgga caaataaata
     1801 tttcaaaaaa tccttgattg atagacaaac accccaaacc aataaacaga tagcaaagat
     1861 atcaagtccc agtacaagtt tttcgaaata tggaagaaga aactgcatca actctgtcgt
     1921 catcctacct ctcctcttct ttgtttttct ttgtcttcta tttcctgatt caagaaataa
     1981 gaaatgactg ttcgaatgat aataataagt gcaagcttca aaatatctgt ccaagtcggc
     2041 ttgataactg actcgatgat atcagcaacg actagtactt ccaagcttaa caaaatataa
     2101 cttgctagca gttttcttgt atctgcatta cgagtattac gttctgcata gtttttcttg
     2161 acatgagacc aatcaaaaag tttgaataga acaagaacga tcccgacaat taagataccg
     2221 atagaacaaa tatttaaaat agcaatgatc cattctagaa attcaattaa atttgagcga
     2281 aacatatacg ctacctactt tcttttaatt tgttcctatt cttttattgt aaaagaaagt
     2341 taccttgttc gtctatttaa aagttgttct aatgataacg gttcgcttaa atttttcttt
     2401 ttcactctcc catttttctc cctccatcca taaatcgtgg aagtatccaa aacaacgtag
     2461 caagttaaaa taaggcttag tccgtcatca gcttttcaaa gaagcactgt ctcggtgctt
     2521 tttttgtacc caaatttcta aaaatagaac catacacctc ctcaaatctt ttcttcttac
     2581 ctaccctatt tttatttaca ggaaaatcag acacttcttc ttttttgtca aaatttcctt
     2641 tttcttctcg tcctatcatt tggcattcta ataaaaacga actatccttg gatatagaaa
     2701 gagaaaacaa ggaggcgttt ttcatgagta aaaaattaat caaagtcggc atcggtttag
     2761 gtttgcttgc ccttggcgct gcatatctag gaaaaaagac aggactattc gaagatgaca
     2821 gtcatttata tgatgaattc gaatcaatct agcatcctat ttagttgaat cactacggac
     2881 atacttggaa gacattggag gaatcacaat gagaaaaagt aaagcaataa tgattggtgc
     2941 cggtattgcc aatatggcag cggctgttta tttgattcaa gaaggcaaat ggaaagggga
     3001 tcagatcacc ttttattcat tggatgatca cggttcaaat gatggtgcac cgaccgacac
     3061 tgtagtggat gaatattgga ataaaaacca tccattagaa aaccaaaaag gctatattgc
     3121 tcgaggcggt cgcatgctta attaccgtac ctatgtagat ttgatggatc tgttaagtcg
     3181 aataccttct gttactgagc cagggatgac tgctgaagaa gatacgagag attttgatgc
     3241 aaaacaccga acctttgaca aagcacgttt gatggaaggt ggcaaaggaa tcattgatgg
     3301 cggaaaactg ggattcaata ataaagaccg tattcttctg actaaactga ttgctatgcc
     3361 tgattcagaa gaagaaaaac tagacaatat cactatcgaa gaatatttca aagatgatcc
     3421 acactttttt gaaacaaact tctggtttat gtgggaaaca acatttgctt tcagaacacg
     3481 tagttctgct caagaattgc gtcgctatat gcatcaaatg atttatgaat tcactcaaat
     3541 cgaacatcta gtgggcgtca atcgaacacg ttacaatcaa tacgaaagta ttatgcttcc
     3601 tttgatcgag tacttgaaag aacaaggatg caaaatcatt ttgaaccgcc gtgtcactga
     3661 ctgggaattc aaagaaacac cgatgcaaga tgaaatcact gtgactggtt tgaaaattac
     3721 caatacactg actcaagaag aagaatacgt tgtggtcgat gaagatacag cagttatttt
     3781 cactaatggg tccattacag attcagctac tttaggagat tacgaaacac cagctgtaga
     3841 aaatatggat tatggtgcag cttctagtct ctggaaaaaa gccagtgaac gtttctacaa
     3901 cttgggaaat cctgacaaat tctttgctga tcgagatgca agcgaatggg tcagctttac
     3961 cctgacgacg aaaaaccatt tgttgttgaa tgaaatcacc cgtatcacga ctcaagaacc
     4021 aggaaatgcc ttgaattcat ttatttcaac cacaccgatc acgccgttag gccaaaaaga
     4081 tgtcaatatg tctattgtcg tgcatcatca accgcatttt acgtctcaga aaccaaatga
     4141 aacagttatt tggggttatt tcctttatcc aagacgacgt ggcgaatttg tggataaaca
     4201 gtatattaaa atgaatggaa aagaaatgct agaagaactg attggacaac tttcaaaagt
     4261 agatcctgga ccagtgaata ttcgtgaaaa agaaacagaa attttcgaca gcgtaataaa
     4321 taatatccct gtctatatgc cttatgcttc cgcgttgttc aataatcgtg ccaaaagcga
     4381 tcgaccaaaa gtcattccta aacattcgac caatctggcc tttacaggag aatttgtcga
     4441 gcagccttat cagatgatct ttactgaaca aagtgctgta cgttctggag aaatcgctgc
     4501 tttccatttt gcgggtattc ctatgtcccg tcttgtcaaa acaccgcgtt acgacaaaga
     4561 tattcctact ctagctcgtg ccgcaaaaaa aatgtttgaa taatcaagag gttgtgaaaa
     4621 agctgccctg catcaagtaa taagaacaac caggctaaaa cagcttctta tgtttttgat
     4681 ttggttggac ttattaccgc agatgcaagc ttttgaacac cgtttaccaa gaggttatga
     4741 aaaagctgcc ctgcatcaag taataagagc agccaagcta aaacagcttc ttatgttttt
     4801 gacttggtta gacttattac cgcagatgca agcttttgaa caccgtttac caagaggtta
     4861 tgaaaaaggc gtttagtttc gagtatgaag aaaaattttg tgaaaatggt tcctcacgtt
     4921 tttgcataaa tttgacttaa taccgagaaa ctgatccctt ctgtcataac ctctttttat
     4981 ttatgtctgt ttctttttga aaacaactat tttttcaacg tttactctct tgccaatcca
     5041 acatcatcta acggatacaa ctatttcaat gattaccatt acactaaaaa atcggaaaca
     5101 cacctctccg aacacaaata cataagacta tagaatgttt catttaagaa aaaaagcgtt
     5161 tcattatcaa aaaggtaaga tctcctttaa aatgaaatta tctgtagtac taataatgca
     5221 gacaatttta tgaatctgaa aggagcatct ccaatgaaag cagtcacttg gcaaggaaaa
     5281 cagaaaatgg aaatcagaaa cgtagatgat ccaaaaatat tagaaccaac agatgcaatc
     5341 atccgtatca cagcaacagc catttgcggc tccgatctgc atctttacca tcatggtgaa
     5401 gcagttttgg aaaaagggta tgttgtagga cacgaaccaa tgggaatcgt tgaagaagtg
     5461 ggatctcaag ttcaaacatt gaaaaaaggg caacgtgtgg ttatcccttt taatattagt
     5521 tgtggtcatt gtcatttctg cctgaatcac atggaaagcc aatgtgatga atcaaatcca
     5581 gatcagttat atggcgggct tttcggattt ggcaagttga acggtaatca ttggggtggt
     5641 caagcggaat atctaagagt tccttatgca gactctactt catttgttgt accagataat
     5701 gatcttcctg atgaaaaagt tttatttcta tcagatattt tgcccacagc ttattggagc
     5761 gttgaaaacg caggagtaaa aaaaggcgat acagttgtga ttctaggttc tggtccagtc
     5821 ggtctattcg ctcaaaaatt cgctgttatg gctggagcag agcgcgtaat cgcagtagat
     5881 ccagtacagc accgtctaga taaagcggct aattacaatg gggttgaaac ttatttctta
     5941 gaagacacgg cacaagctgg aacagaattg tatgaactgg ctaaaggtgg cgcagatgtc
     6001 attatcgatt gtgtaggaat ggacggatta gagcctgtca aagaaaaagc gaaaaattta
     6061 gtaagcttac aaagcggcac aatctctcca ttacagatgg catcgcaagc tgtcaaaaaa
     6121 tttggaacag tccaagtaac tggtgtttat atgactccag cttcttctta cccgctgcaa
     6181 gaatttttta tgcgggatat cgctgtcaag catggacagg caccaattat ccatctaatg
     6241 ccaaaaattt acgcaatgat tgcagaagga ctattcgatc ctacacaaat cattactcat
     6301 tcgctgccac tatcagaagc agcacaagca tatgagatct ttgacaaaaa agaagataaa
     6361 aatataaaag ttgttttgaa accttgaatt cagtaaaaaa tacaggtgat tttctttatg
     6421 cccaaaatca aacgaaatac ccatgagatc gaaataggaa aagaagaaga tatcgaaata
     6481 cgaggacgtc gcttccgctt ctatttccaa aatcgttaca cattgatctc actggctgtc
     6541 gatttattaa ccgggatttt ctatattttt ggaagccttg cttccttaac taaaatcccc
     6601 gatcgatatg gcacctattt ttatttggct ggtgctattt tcttgacgat tcgtcctatt
     6661 ctcagaatcg taaggaacat ttttatttac gacgaaaaaa aagcaatgaa gagaacaagc
     6721 aaaaaaaaag aggcatgaga atttttcaaa tctcatgcct cttttttcac cctatcgtat
     6781 cactttgata cagacgagcg gtcgtcccac tttcttcctt gaccattgtc aaaaatgtcc
     6841 actcgtatcg ttcataaaac gtggtatgat ctgtaattaa gtaaagtttt tcgtacccaa
     6901 gttgatgcat ttcttttctg atttccgcta atatccgctt ggctatcccc tgcttacgct
     6961 tttccggttc aacaaacagg gcacaaagat ttggtgtcag atcagggcgg ttatggaagt
     7021 cgttttcaat gacgcccgct cctccgacga tggtttcttt ttcatctaag accacatacc
     7081 attgaggaat actttcctta ttagcgattc cagtacgaat actttcctca tacacaacag
     7141 caggaactcc ccattttgat gaaaaccaat tcgcgctttg ttttactaat tctgggtgtt
     7201 ctcttaattt gatccagtta tatgtaacca atgtttaaac ctccgactaa aataaaacgt
     7261 ttacaaaaat tatacaagtc tttatgaaaa ttagcaatga caacaatagt gatgtaaaaa
     7321 aaaaaacgga aaacaatcac ttgttctccg ttttttgatt aatcaagcat ctaaagcttc
     7381 ttttcttctt tcttggatcg tagctacctt gacgaacggt agatagatca aaatagaaag
     7441 aaccaaattg acagcggcta gtacaccacc tgcaatactt tttgttgcca atacaccgcc
     7501 aataattggt ggagttaccc atggaggcat aacggtagct gctggcacta aacctgtaga
     7561 agtcgcaaag taagcaacac taacta
//
LOCUS       DAHHZJ010000135         7564 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.137, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000135 DAHHZJ010000000
VERSION     DAHHZJ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.137"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            69..503
                     /locus_tag="NBL02_002252"
     CDS             69..503
                     /locus_tag="NBL02_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD2572928.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(581..1114)
                     /locus_tag="NBL02_002253"
     CDS             complement(581..1114)
                     /locus_tag="NBL02_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HCD2572929.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(1208..2386)
                     /gene="mutY"
                     /locus_tag="NBL02_002254"
     CDS             complement(1208..2386)
                     /gene="mutY"
                     /locus_tag="NBL02_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCD2572930.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2379..3176)
                     /gene="recX"
                     /locus_tag="NBL02_002255"
     CDS             complement(2379..3176)
                     /gene="recX"
                     /locus_tag="NBL02_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCD2572931.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            3283..4665
                     /gene="rlmD"
                     /locus_tag="NBL02_002256"
     CDS             3283..4665
                     /gene="rlmD"
                     /locus_tag="NBL02_002256"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCD2572932.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4839..5417)
                     /locus_tag="NBL02_002257"
     CDS             complement(4839..5417)
                     /locus_tag="NBL02_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HCD2572933.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5739..6209
                     /locus_tag="NBL02_002258"
     CDS             5739..6209
                     /locus_tag="NBL02_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HCD2572934.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(6317..7543)
                     /locus_tag="NBL02_002259"
     CDS             complement(6317..7543)
                     /locus_tag="NBL02_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCD2572935.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT         2197 a         1509 c         1320 g         2538 t
ORIGIN      
        1 gaaaacttgc catacttttt atttgtttta ttttccagta tactatacga aaggaaggtg
       61 gctagaccat gaaagttgta caaacaaaag atacaatgag caatatttac ttagatgctg
      121 tacggatcag gcatcaagtt tttgtagtcg aacagggtgt gcctttgtct cgagaaatag
      181 acaaagatga agcccattgt atccacttcg tcctgtactc agataaaaaa gaaccgcaag
      241 gaactgtacg tcttttgcct ttagaaaacg gaaaaatgaa attacagcgg atggcgattc
      301 tttctgaata cagacaccaa gggttaggaa aaatattgat tgaagaagca gaaaactttg
      361 ctaagaatca aggctataat acgattttat taggggcaca gtccacagct gaaacttttt
      421 acgaaaaact aggatataca gcttacggcg atccttttga agatgctggc atgcctcata
      481 tttatatgaa aaagacttta taaaaattta tttcgaaaaa agcaaaaaac agcaagcttt
      541 tgagaaaaat ctctcactgg cttactgttc ttttttgtgt ttacttacgc gacaattgct
      601 tgtaacggtt ataccaaata tcaatatagc cttccgagaa cgggccatct ccattattga
      661 tccagtcaac cagaatcttc acattttctt ttaggatgaa gtcaatatcg ttcgggtaat
      721 tcatcatttt gctgtgcatc tcatattcat cgacatccaa tagtcgtttc tcgccgtcag
      781 gaaatacctt gatatccaag tcatagtcga tatatttcaa ggcctcatcg tccagcaaaa
      841 aaggtgaagc tagattacaa taataggaaa cccccttttc tctgatcatt gctattacat
      901 tgaaccagta ttttttatga aaataaacaa tcgctggttc gcgcgttacc catcggcgtc
      961 catcagattc agtgactaat gtgtgatcat tcacgccgat catagagtgt tcgcttgttt
     1021 ttaataccat ggtatctcgc cacgttcgat gcaatttgcc gtcgtgcttg taactttgga
     1081 tcgtcacaaa ctctccttct tttggaactc ccatagcaac ccttccttaa gcagcatttt
     1141 gtgctttgtg tttgggtgtt cattacaccg acatcataat tattatatca ataaaaatga
     1201 gctttgccta gaaaggattc ctattgtttc gatttttctc taactgatct accagtttca
     1261 tttggacctt aggaaaaacg accgaatcaa atgcatcagg cttcaaccac ttcgaagttt
     1321 tgttctcggt aaattcttgt tctgctccct cggttgcccg tccgtaaaaa aggagaacat
     1381 gccatttcag atgactaaaa atatgagtaa cttctcctaa atgtcgtgtc tgccaaacaa
     1441 agagttcctg cttttcaaat gggagatttt gctcttcttc tgctactagg tcatcaaaca
     1501 gatcgatttc tggctttgtt tcccattctt ttttcaatcg ctcatactcc tcttgtgtca
     1561 cttccatcat tggaaatgtc cacatattcg ccagcaattt ttggctatct ctttcttcaa
     1621 aataatacgc tccagaatga ttttgaagag ctgcactgat ataataaacg tcttttggct
     1681 ttgctttttt tgtttttaca ggaaaactcg tttggattcc tcgtttgtta gctaaacaaa
     1741 aggcttggat cggacaagtc tcacactttg gagaagttgg tgtacagata gctgaaccta
     1801 gatccatcat tgcttggttg aattcacctg gatgcttttc atcaatgatt ttccgcatcg
     1861 cttcatcaaa gattttcctg ctagaagctt ttgcgatgtc tgcttcaatg caaaacaatc
     1921 tgctgactac tcgcatcacg ttgccgtcaa ctgcaggttc tggaagtcca aatgcgatac
     1981 ttgcaatcgc gcctgtcgta tatggtccta ttcccttcaa tgagctaatt tcttcaggcg
     2041 tttgcggcat ttccccatca aattctgaca tgatctgttt agcagcagct tggatgttcc
     2101 gggctcttga gtaataacca aggccctccc atgcttttaa aagcttttct tctggagcat
     2161 ttgctagctc ttcaattgta ggaaaccatt ccatgaaacg ataaaaataa tcgatcactg
     2221 tgtctactct ggtttgctga agcatgatct cagagatcca gatacgataa ggatcacgat
     2281 tatagcgcca aggcaggttt cttttttctt gttcatacca ttgaatgaac tgatcttgaa
     2341 attcttttgt ttctttatct gtccattttt cccattcttt attcactttc ctcttccttt
     2401 tccgtaatga atcgctggat catatccaaa tcaaatcctt tttgatataa gctttgcttt
     2461 acttttagat ttcttttttt aggttcaaac cttaaatttc ttcgccatag tttatctcct
     2521 tgtttgacta aataatcata ttctatttct tgatctgctt cttgcggaag atcagctatc
     2581 gcttgttgga tcacttcttg agaaaagcct ttcgtcatca atgtttgttg gatttttctc
     2641 aataattctt tttggctttt tccgtgattt tttccaaacg ctttttcggc tgtctgatgt
     2701 gcattttgaa cttggtcttc aaatgtatat tggctgagcg cttgttcaat gatttcaggt
     2761 ttcaaccctt tttgctgcat ctgctgcgct aatttctttg gtcctttgtc acttaagcgc
     2821 atattcgtgc gcacataact ttccccatag accaaatcat cgacaaggtc caattctttt
     2881 aagcgggcta cgattttatt tcgatcttct tgaaggattt ccttttcttt taagtatgtt
     2941 cggatttctt tttccgatcg caactgataa cttaagtaat tcattgctag gtgaaaaccg
     3001 aaatcagctc cgctactctt cttgatctct tggatagttt cttcgtctaa ttcccgtcct
     3061 tttaataatc gatagcgtac taatacatct tcagatactt gtaaagcttc tccttcagat
     3121 aaatctacgc gatagaaagg gcctttgcct ttactgattc tcgtaacggt taacataaac
     3181 atcctcctcg aacatatatt ctctatcata ccacagaaaa tccccgtaat caggtattat
     3241 atgttagaat aaaccacaga aaaaagaaaa agggagatac ttatggcaga aacaattgtc
     3301 aaaactggac aaacggttcc tttgaaaatc aagcgtttag gcataaatgg tgaaggaatc
     3361 ggttatttca aacggttgat catatttgtt ccatatgcac taccaaaaga agaagtacta
     3421 gtgaaaatca cgaaagcaac tcctcgctat gcagaaggag aattaataaa agtcaaaaaa
     3481 acaagcaaag atcgcgtagt ggcaccatgt cctgtttact atgaatgcgg tggctgtcaa
     3541 ttacaacact tagcttatca agctcaattg gattttaaaa aggatctgct tttgcaagca
     3601 ttggaaaaat tcaaaccagc aggctttcga tcttatcaac ttttgccgac gatcgggatg
     3661 gatgagcctt ggcattatag aaacaaagca caatttcagt tacgaaaaaa taagcagact
     3721 caaaaaattg aagctggttt atacgaagca aactctcatc agttagtccc tataacagac
     3781 tgcttggttc aagaacctcg aacacaaaaa gtaatgaata ctgttgttca actattgaat
     3841 aaatttgatg cacccatcta tgatgaacgg acaaacagtg gaatcttccg cacaatcatg
     3901 gttagaatcg gtgttaaaac tggggagtta caagttgttt ttattactcg cagtaaaaaa
     3961 ttcccgcaaa aaaacgcaat gatccgtgag atcaatgaac gactgcccga agttgtttca
     4021 atcatgcaaa atgtccaggc acaaaaaacg tctctcgtga tgggggatga aacgatccac
     4081 ttatggggaa aagaagccat tgaagaacaa atcaatgaag tgactttcga tctttctcct
     4141 cgtgccttct ttcaactcaa tcctcagcag accgaattgc tttatggcga aggaatcaaa
     4201 gcattagatg tccagcctaa tgaaacgatc gtggatgctt attgcggagt tgggacgatc
     4261 ggcttaagtg tagcaaaaca agccaaagaa gtacggggaa tggataccgt tcctcaagca
     4321 atcgctgatg caaaaaaaaa tgcggaaaga ttaggatatc acaacactcg atatgaagtc
     4381 gggacagcag agcaagttct gcctaaatgg ttgaatgaag gttttcagcc tgatggtatc
     4441 atcgtagatc caccacgtac gggtttggat aacgcattga tccaatcatt attgaaatac
     4501 cctccaaaaa aacttgttta tatctcctgt aatgtttcta cacttgccag agatctggtt
     4561 aatttaagta aaaagttcaa gattgaatat ttacagtccg tcgacatgtt tcctcagact
     4621 gcacgttgtg aagttgtcgt gaagatgacg agactcgctg actaaatgaa agaggttgtg
     4681 aaaaagtctg ccctgtatca agtaataaga acagccaaac aaaaatagct cttcatgttt
     4741 tatgaatact gtacttgtga ccgaagagtt aacaggagga cactatttat ttaaaaaaag
     4801 ggttgtgaca tagtttttgt cacagccctt tttgattatt attggatatt cacgtctatc
     4861 acttgataaa atgcatttgt cgtatcgctg atattccata ctgctaaaat cacattatat
     4921 ccttttcgat cctgcggaag tgtgatcgtt tggttgactg tttttggtgg aacagttgct
     4981 ttgtcgtcta tttttgtgat caattcaaaa tcactaaatt tcaaaggttg attaggattc
     5041 caagttggtt tggtaatgta gtaatcccaa gtacttgtcc gatgttgtgc tgttaaattc
     5101 caagtaatat ttaatgttcc tggattaacc atgtttttat gccatctgtt agatgtctgt
     5161 tcatctaaag gctcaaagcc gctgactcct gcactagcaa tttttccatc aatgaatgta
     5221 tttttggtag cttcgatact ttgcggttcc cattgtgctc ttccgacatt ttgatttaag
     5281 tttcctgctg ctgatgttcc taggtatgca cggcttcctg gagaagtaat ataaccatgc
     5341 gcaaaacctg gaatgctaaa accgaacaac cctaaactaa ctaacccaat aaacaataac
     5401 gtactaatct ttttcatgta gaaacctcct tcatttgata accacattgt aaacgcttta
     5461 ttgtattaac acaaacatta tgcttttata attttatgtt gaagtaaaaa aggtgaaaaa
     5521 agtaagatgc agattataac agaagtagac gagaaaagta caaatagaaa acatgagaat
     5581 caaatcatat gatctttttc aaaaacatca ttcatataaa caaatgttca actttttaat
     5641 aacaacaatt atcaattttt ttcttacttc acaaactaaa tacaggtctt gctgtgagcc
     5701 ttcatggtaa actaatatta tagaaaggag ttagtaaaat gaagatgata aaagatattc
     5761 gtttttgggt ttgtgttata ggaattgtca tattaggctt tttatcaggg ttgcttagcg
     5821 gaaatcccgg aaaatattac tattctttgc aacttccacc atttgcacca cctagctgga
     5881 tatttgggcc gatgtggacc ttactgtata tattgatggg tatttctctt tatttgttac
     5941 ttaatcacaa caacaaaaaa cagcgaaaca atcttgtcgg tctttttgtg attcagttca
     6001 tttttaattt tatatggtct gctctatttt ttaatttaag aaatattttc attgcagcaa
     6061 tcgatattac acttttagtt attttcttgt cagtgcttat gtatcagttg tggctgcatc
     6121 atcgcctggc aatgtggctc atgattcctt attatttatg ggtactattc gcaactctat
     6181 tgaattattc aatttatttt ttaaactaag cgctaaaaag agacgtcctg taatttaaca
     6241 catagaaaaa gtgccaccta agttagattt tttgtctaat ttaggtggca ctctttatta
     6301 accaaactat ttttttctag tacccacttg ttgttccata ctcattattc tggtcataac
     6361 cataaccatt ttgttcctgt ccagcgtaag gatcataact ttgagtatct tgacttggtg
     6421 tatagtactg acttgtatct tctgtttgct gataataggt attcggatct tggtactgat
     6481 catatgtgtt ttctgaatag gaagaataat cggtgtaccc ttcactattc gatccatcac
     6541 cgtttgtcga atcgccatct tccgaatcat caaagaacat cattgaagca ttctcatttt
     6601 tcaaattagg taatgtatcg cttgttttta tatttaattg tttcttcagt tcattttgga
     6661 tcgaaagaag atcatttttt cctaaaattt gatagtagat atcattgatc atttgtcctt
     6721 cgccttgcaa ctggtcttgt ttgattgttt caaacgccgg ggtgtagttc gtcgctacat
     6781 caagcatgtc atcccaatct aagtccgtcg tcacattgtt ctcaactgct tttaaaattt
     6841 ttctataatt gcttacgcca tctaaactta aaactttatt gacgattttc gtcactactt
     6901 cccgctgacg acgttgtcga ccgatatcgc cttctggatc ttcgtgtcgc attcgagaat
     6961 aggctaaacc tttttctcca ttcaatgttt gttcacctac tggtacatgg acaccatcca
     7021 aagtaaagtc aattttatta ttcactttga tgccaccgac cgcattgatc aaatcttcca
     7081 tgccatccaa attgatcgtt acataatgat caataggaat atcgagcagt tgctcaattg
     7141 aatccatggc catttcgaca ccaccaaatg cgtaggcatg atttaattta tctaccgttc
     7201 cataacctac gatattcgta taaatatctc ggtccaaact gatgatcgtt gatttttttt
     7261 gtttcggatt aactgttact accatcatcg tatcagaacg tccttgttcc gttcttccta
     7321 atccaccggt atctaaacct aatagcaaaa ccgaaaaagg ttctttgtca tcaatgctta
     7381 ctctctcaga acgtttggag gatattcgat tagagttttt agaaattttg tttactgttt
     7441 ggctcgcttc tcccataaat ctgattccgt atgctgataa tccggcaatc agcaaaacga
     7501 atacccctaa caaggttaat agaacttttt gccatcgctt cataatacac ctctgtttca
     7561 catt
//
LOCUS       DAHHZJ010000136         7564 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.138, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000136 DAHHZJ010000000
VERSION     DAHHZJ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.138"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(1..779)
                     /locus_tag="NBL02_002260"
                     /pseudo
     CDS             complement(1..779)
                     /locus_tag="NBL02_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            complement(862..1971)
                     /locus_tag="NBL02_002261"
     CDS             complement(862..1971)
                     /locus_tag="NBL02_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HCD2572936.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            complement(2265..3290)
                     /locus_tag="NBL02_002262"
     CDS             complement(2265..3290)
                     /locus_tag="NBL02_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCD2572937.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            complement(3535..4806)
                     /locus_tag="NBL02_002263"
     CDS             complement(3535..4806)
                     /locus_tag="NBL02_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCD2572938.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            complement(5090..6037)
                     /locus_tag="NBL02_002264"
                     /pseudo
     CDS             complement(5090..6037)
                     /locus_tag="NBL02_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            6049..6522
                     /locus_tag="NBL02_002265"
     CDS             6049..6522
                     /locus_tag="NBL02_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCD2572939.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            complement(6695..7249)
                     /locus_tag="NBL02_002266"
     CDS             complement(6695..7249)
                     /locus_tag="NBL02_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCD2572940.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
BASE COUNT         2377 a         1461 c         1111 g         2615 t
ORIGIN      
        1 tgtttataga ataaataggc taatgattgt gatttaaaca tggcggtact attaggagta
       61 atcatcataa aaataacatc aaatacttgg aaagcattga taactgttgt tacaactaca
      121 aaaaataatg ttggtgtaac taatggtaac gtaatgttga aaaactgctg gattggacta
      181 gcaccatcaa tttccgctgc ttcataataa tctttaggaa tttcttataa gccggctagt
      241 aataacacca tgttataacc aatagcactc caaactccta caatgataat agaataaatc
      301 gcaatcgatg gatccgttaa ccatgctggt ccatcgagac ctattttagc caacatgtta
      361 ttaatcagtc cataatcatt attgaacatc cagcgccaaa tcattgcgac tgcggatggt
      421 gcagcaacga ctggtagaaa ataaattgtc cgataaatcg acaatccttt aacttttcta
      481 ttcattaaaa ctgccaccaa caaggataat acaacaatac taggtatcga aaccactgca
      541 taaattaatg tgtttaataa agactgaaaa acttcaccgt ctgcaaacat tttttggtaa
      601 tttcttaatc cgacccattc agtatcaccg aaccctaccg ttttttgaaa acttaaaatt
      661 gctgtttgaa ttgctggcca tatatttaat aaaattaatc ctaatgccgt tggaaaaata
      721 aaaatatagc cccatatcac ttgatcttta cgatattttc caaaaattcg atttttcata
      781 atatccccct ttttggtatt catccattaa tatacagacg aaatagaatc ctttcgcctg
      841 tatattgtat aattaactaa tttaatttcc gctcaatatc gcattgactt ctttttcaat
      901 ttgagcggtt ccatcttcaa cagttacttc accagacaga atacggttaa taatttgaac
      961 ttctgcttgt tcagctcgta atccatcttt attgtatgga cgagggactc catactccaa
     1021 catttttcca aaagcactca aatcaattgt atctgaagaa gcagcccact cgtcttcaaa
     1081 gccttttagt gctgggattg atgcccccgt ttccgcttgg attttattgg cgtcctcact
     1141 agctaaaaat gccattagtt ttttcacttc ttctgggtat tttgtactcg cactggcagc
     1201 ccaaccaagt ccatttgtaa ttgttgcatt aacttttccc ttaggtaaca cggcagaagc
     1261 aacattttca actagatagt catttttcaa aaatcccggt gcaaaccagt ttccttcaat
     1321 catcattgct aatctacctg attgaaaacg acttaactta tcaggatctt gtaattctgt
     1381 aattgttggt gaagcatgat caacaagtgc aatatctcga taaaaagcta aagcttcttg
     1441 tgcttctggg gtattcaaac ctgattttgt attgtcatcg gcaaatactt ctcccccatt
     1501 ttgataaata aatggccagt agccagtctc tgttccaata tgtgatgcaa aaccatagat
     1561 gccttttgat tcatctgtta aatcaattgc agcttgtctg agtgtttccc atgtccatgt
     1621 ttcatctgga taatcgacac ctgcattatc aaataattct ttgttgtacc atagccccac
     1681 tacagaagca tctttaggga ttgcgtatag ttcgtcttca agcgtataga aatcagcaat
     1741 tccttcagta aagtttccca tgtcaatatc attttctttg ataacatcat caagtcccat
     1801 caaagcattc tgtgacatat atttatagga ttcgctagag tgcatggtta ctacatctgg
     1861 catattacct cccgttgttg cagcttctaa tttaacccaa tattgatccc aaggcgttgt
     1921 ctccatttct actttgatat caggattttg tttttcaaat tcatctaaca tttttcttag
     1981 tcctggagct tggtcagtat ctcaactcgc atagcttaaa gttacttgcc cgtctttaga
     2041 attactatct cccccaccac atgctgcgag taatgtccca gttaaaacag ctatccctgt
     2101 taaagctatt tttttgaaat gtttcatctt aattcctcct gttttgaatg cgttttcttt
     2161 ttgttaacaa caccataact gtttacttaa tgttttgtcg agatttttta ctaaaaatat
     2221 tagtaaacaa aaaagtaaac accctttttt ggatgtttac ttttttaatc gatatttttt
     2281 tcactttctc taacaataat attattagaa acaatcactt tgatcgaaac atttctaact
     2341 tcttttattc gttcattaac catgcgaact gctagttttc caatttcttc tgtattcaca
     2401 ttcaccgtat ttaatgcagg tgttaaaaat tcagcaactt caatattatc aaaactcaca
     2461 atcgaaatat cttctggaat atgtagacca tttttttgaa gtgaacgata aacccccaca
     2521 gcaataggat catttcctac catgatagca gtcggcaact tatctttagc atgttttttc
     2581 aaaagtaaat ctgctgcctg cattccgtca agtgtggtcc agccttctaa ataaatttga
     2641 ctatattttt ctaatccttt ttgtttcatc cacgtctcat aggctatttt tcgataatca
     2701 tcatctgaca cgtgtttatt tccttggcta tctaaacgaa cacgtcctcc tccgataaac
     2761 gcaatgtttc ggtgtccttt tttatacaat cgatctaaat gctgattcgt tgcttttgtt
     2821 aaatcagtat aaactgcatc tacttcagct tcaatactag ggtcatcaat cactacgaca
     2881 ttcgaattaa ttgctgatac ttcttcaatc acagaagata gcacctgccc aataatcaac
     2941 acaccttgac attttagcaa atcgtcttgc tccaacggtg tttctgacaa acgaataatt
     3001 ttcttcaaat tgactttgct ttgttctgct tcgacttgaa tccctctgcg aatcgttcga
     3061 aaatagggat cttccaactc atctacttca gaaactgtcg ttattaatgc aatatttgtt
     3121 aaattttttg caacgggact ttttttgaca taatgataaa aattaaccga ttctgcaatc
     3181 tttcgtttgg tttcatcggt tactgaaagt gtcggatcct catttagcac gcgcgatact
     3241 gttgcaatgg acacacccgc ccgttttgca acatccctaa ttcctaccat aatagctcct
     3301 cttttcatat ttgaaagtgt ttactaacat ataatcatac attttgaaaa taagcaaatt
     3361 tcgttaaaaa taggcactgt aatcataatt aaaaacaaga aaaaatatcc gaacaattta
     3421 gagtaaataa tgctaattct agtgaatttg cgacgaggaa ctgctagatc acagttcatt
     3481 ctctctatcg ttcggatatt ttattgttaa ctatacttat ttcacagagc ttttttattt
     3541 gattttgtga acagagtctc gagaaatcaa ttctccctct aatagtagta cttgattttt
     3601 tatttcttct tgtttggcca gtccaatcag ttggtgaaaa gcaatcgccc ccttgttacc
     3661 tggatttagt cgtattgttg tcaacgcagg atccattaat tctgcaaaag taatatcatc
     3721 aaatccaata atcgataatt gttccatcac tcgtttattc ttatttttta aaaattttac
     3781 taatttagcc gctaaataat ctccagtaac aacaattgca gtaaattcag ctaattcttg
     3841 ataaattcgc tcaaattcag agttgttata tatccaatgc gatttaaagg ttaaatgata
     3901 tttctttaat gtatcgatat aagcttgatg tcgttcttga ataacacttg gttcacctac
     3961 aatgtagtca aaagaaagaa acgctatttt ctcatgacca taatgaatca aatgttccac
     4021 tgccaatgta ctagcttggt aatcatttgt atttacaaat aaacgattca tttgttgttc
     4081 aagttctctt aatgtctctt ttgaaagatg cgtatcaata taaacaacag gaccatcaaa
     4141 aacgttattt aattccaaaa attcttttac acgtacacca acaacaatga acccctcaaa
     4201 acgccaattt ctctgaatag ttttaacatc ttctgctgaa ttaacattat gcaccaatac
     4261 aaaataacca tgattctttg cctgttcctc aattccagac aaaacttctg cggtaaatgg
     4321 atcagaaaat acaaaatcag cattatcaat agccgaaaaa taaagcaatc ctattaaaaa
     4381 tgcaatcgtc ttaccgattc cctgacagac agaacacatt ccactaggat cattaaatga
     4441 ataagcattt gcatatccta aactaggtgt tgcaaaacat gaaaacaaca cacgaaataa
     4501 cgcattgatg ttagtaatcg tccctaacgt tgagcgcgca ctgccaccaa tcttcttttg
     4561 attgaccaca atcgccgtag aaagattatc aatctcatca acctctggac gactgtattt
     4621 aggtaaaaat aaacgggtaa aactatcata ggtgtcattc agttgacgac cagcttcttg
     4681 ggtaatagta tcaaaaacaa tcgatgattt tcctgaacca gatacgctgg taaaaatact
     4741 aattttattt ttgggaattt tgagactgac atttttaagt tgtttctctt gcgtggataa
     4801 tttcaatata ctcgttcatt tggtactccc ctttattttg aaatagttga gaatgtttca
     4861 atgagacacg gtatttttgg cagatgttta gtttacctag aaagaggtat aaaaatcaag
     4921 aaaaaaagag aggctgactc ataagtagtt ttattagtcg aaaatgatag tttataaaaa
     4981 taggaaccgc atcaaatcaa cattctttag aatggatttg atgcgttttt ctttattttt
     5041 tgacttatga gtcaacctct tactcttcgt catggatttg attagctaat tattgctcca
     5101 cttgttcacg aatgaaattt aagtactgcg gtaaaaataa aggaatttct tcaaagcgtg
     5161 gactttgatt ttcatacgct tttgcatcta ggtttccttc ccctgtagca atttcaatca
     5221 ataattgatt tgggtcacga taataaagtg atacaaaaaa ttcacgatct tttattcctg
     5281 aatttataaa attgcgctct tgtaattgtt tgtcgatgcg ttgtaaatca gatttatttt
     5341 ctacgccaaa cgcaacatga tggacgctac caatcccttc aatggcaact ggattctcac
     5401 gatcttgaat aatctgaact tcttgataga actgagtgtc ttgattttct aagattgtca
     5461 ctcgttgatg acccgaaaag aaactgacgt attctttgtg ctaccagcct accgacaata
     5521 actctcgttc agtcgcatcg gaatattgta cgcgtagttg aatggcatca attcctaaaa
     5581 tggcatgctc tgtagggatc tggtctgtga catacgggaa ataatcatca atatcttcaa
     5641 attcgcgtaa gggaactaaa gctaatcgtg ttgcatctgg cccttcaaaa cgtaagatag
     5701 gtcgtccaga aaatgattcg attccataat ggcaaacgcc ttgtgtttca aaatatattt
     5761 cccaaaaatg caatgatgct tcggaaggaa cttttagtat cgttcgttca atcgtattgg
     5821 ttccaaaaaa ctggttgttc ccttcttgta cttcaaaaaa agtcagttca gttcccacac
     5881 ggtggtgatt atctgaaaaa aacaggtgat acatggtatg atcgtcttga ttaattgttt
     5941 tcattagtaa ttttaaaccg agtatattat gataaaagtc aaatgtcggt cgaataaagc
     6001 gattcacaat agaaatgtga tgaatacgtg cgggcataat atctaaaaat gagaatcaaa
     6061 cctataaaag atggtgaaca acttttgttt tcatcagaag aaaataagcc cctttattta
     6121 gactatgtaa atcacaattt gaaaatcatc attaaagaga ataacttaaa acatatcaca
     6181 cttcatggtt ttcgtcatac ccattgtagt ctgctctttg aatctggtgc gtctgtcaaa
     6241 gaagtgtatg ttcgacttgg acatacagat attaaaacca ctatggatga tatatacacc
     6301 catatcgcga aaaagcagac agaggaaacg gcaattcgtt ttgcagattt catgagttct
     6361 acagaggaaa agcaaggaag tttgcagaag gtcaaaagta tggtcaaaaa cgaaaaaagc
     6421 agactatccc tattagatag tctgctaaac gttgagttat cagtattttt cttattccgc
     6481 agcgtttgcg tttttgagac cgtattgatt ctcttttttt aatttcatcc ttttccaacc
     6541 taaaaatgtt atttaattac ttagtttata taacacgaac ctataccaag taccagtaac
     6601 cccagctttt attccgcgaa ctttatgaaa taaaatcgtt ctagataaaa atagctttta
     6661 atctttgtct ttactgttta taaaaatttt taaattatac acctagttta aatccacgaa
     6721 gtgatgctcc tatttcatct tcagttatac caatgtacct ttttgtaatt gcttgcgagc
     6781 tatgattaaa aataaacatt aaggtagcaa tgtctctagt tttcttgtag tagtgatacc
     6841 caaaagtttt tcttaatgta tgtgttccta tatcatctcg tcctaatttt tctgctatat
     6901 ctgcatatat ctgatagaca cgatcccttg ttatatgtcg accttgttgc cgactcggaa
     6961 ataaccaatc attttcttct ttaccttcag tatataataa aatttcttct ctcaaattat
     7021 ctataaaaat cggttttcgc ttacctgttt tatgttcaac aataataggc ttacttttgc
     7081 ctcgtatatc tccaactttt aatcctataa tatctgacat acgtaaccca ttattgattc
     7141 caaacaaaaa taaaaaacta tctctttccg gattacttgt tcttcttagc caaaactgaa
     7201 aatcttgtat ctcttcattt gtccttaacg gttgtacatt atagctcact attgtttccc
     7261 ttcttaaaaa tattctgagt taatgattat gtatatattt taccataatc attaacttat
     7321 atatataaac ttttgaaagt caataaaatc aagggattta gtttatgatt ttatatcatt
     7381 tcagtaactc agaatattcc gacttttttg tacaaaaaaa ttaaccttac ttatatgaag
     7441 attctaagct cttatttaaa ttttagacgg gcagttatcc gtagaagttc ttagaatagc
     7501 taatttacat aattaaatta tatgaaattt aagtttgaac aatatactaa gaactcccct
     7561 tact
//
LOCUS       DAHHZJ010000137         7483 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.139, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000137 DAHHZJ010000000
VERSION     DAHHZJ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.139"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            143..1534
                     /locus_tag="NBL02_002267"
     CDS             143..1534
                     /locus_tag="NBL02_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD2572941.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(1607..2254)
                     /locus_tag="NBL02_002268"
     CDS             complement(1607..2254)
                     /locus_tag="NBL02_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HCD2572942.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(2262..2948)
                     /locus_tag="NBL02_002269"
     CDS             complement(2262..2948)
                     /locus_tag="NBL02_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2572943.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(2954..4027)
                     /locus_tag="NBL02_002270"
     CDS             complement(2954..4027)
                     /locus_tag="NBL02_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2572944.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            4249..4923
                     /locus_tag="NBL02_002271"
     CDS             4249..4923
                     /locus_tag="NBL02_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD2572945.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            4928..6268
                     /locus_tag="NBL02_002272"
     CDS             4928..6268
                     /locus_tag="NBL02_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCD2572946.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(6417..7403)
                     /locus_tag="NBL02_002273"
     CDS             complement(6417..7403)
                     /locus_tag="NBL02_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HCD2572947.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         2319 a         1386 c         1318 g         2460 t
ORIGIN      
        1 gctaaaggga atcacaccaa ttttcatacc aattggtttc atttatgaag ttgattcctt
       61 cgattaatga gcgtttcatt gaaagaaaag ggttgaggga gaaaaacata tgatcaggtt
      121 gttggaatga aactatagaa agatggaaaa aatcattcga attctgtcca tttctgacaa
      181 gtggatgact aaaaaagaac ttgccaaacg aattggtagt tcagaatcta catttgtaag
      241 gtatatcgaa gagatcaaaa tccgctgggg aacggatgta acgattcaat cttcacaaaa
      301 attggggtat cgtcttgaac atttgaatgt accagcttat ttaaatgttc tgatggaaat
      361 gtcgcaaagt tctacaaaca gtcgtttatt aaatgagatt attgtcaatc cgggtaaaac
      421 gattgagtat tattgtgaaa aaatttccat cagccggtct tcttttacac gtaagttgaa
      481 acaatgcaat caaattttag aaagctattt attaaaagtg atcgttgacc aaggttttca
      541 gttaactagc cttaaaagtg agctccagtt gcgtatcttt gttacctttt tctttttaag
      601 ttactacgga agacgcacgt tgccatttcc aatcgaaaaa aagaagatca aagaaattat
      661 ggtcaagaat aattgtcaga tgaatttgtt tcaggaagaa agcccatatg agcaatcttt
      721 ctttattatg tattttgttg tgcatctcat gagagaatcc caaggaactc catttgaatg
      781 gaaagctgga gactcttatg tacagttgat aaatatcgat aaaaaagact tcttaatctt
      841 gaaaaatctc ttaggaaaat tgaagtacag cagctatata aaagtagtga actattttgt
      901 caatacgatt ttttcagaat tttcggagaa agaaaaaaat gaaatcagtg agcagatacg
      961 aaaaaatatg tcctactgtt cgttttcatc cgctgattgc aaagaaaaaa acgaagaata
     1021 tgttgttcaa ttattaacgc aaatttattt tttctcccgt attttcccat acgatacgac
     1081 tcatttgacg atccgtgcaa cttactttgc acagcagatg caacaccgcc agatacgtct
     1141 atatgctgga tttcgtgcag aacttcaatt ttattccact ctactgaatc agaatctgat
     1201 ttattattta tctagctata tcttttggat gcttatcggt aatcctgaaa ttcaccatta
     1261 tacttctgat aaaaaaatac tgattatcag cgacttaagt cttcgacata gtcaatatat
     1321 tgaagaatat atcagtgata ttcttgctgt gcataaaatt cactctgaga caactgctat
     1381 tactgaagat cagctgtcga aatacaatct tctcgagtat gatctgatcg ttacgaatca
     1441 acccattttg aatgcccatg ttcctcatat attgattgat gatagtgtgt cattcgcaaa
     1501 tgaagaagaa ctcatccgct tattcgagtt atgaataatc aaaaaacagc tatgtataaa
     1561 ccaccctgat aaaaatttca ggacaaatac acatagctgt ttctttttat tgtattcctt
     1621 cagcaatcca acgagattta tagcgaaagt cttttaacaa ctcttctttt gtccaattat
     1681 ttgcaatgag atccgtcaaa gtgttcatta cgagggtcat ggaaaagtat aggaaactaa
     1741 ctgcttcttc cgacgattga atggagaaat gattgattct taataattcc agccattttt
     1801 cgttcattgg agaatcagcc gatagttctc tacgtttggt tgtctgctga tcatttcgct
     1861 gggttaacaa gtataacctt tgccaataga tactttcgtg atctaagtcg cagcaccact
     1921 ctaaatattt ttcgatcccc aaaaagaaat gttgtttgtt ttcgctaatg aattttaaga
     1981 tatcttgatg gactatgtga gaactgcttt tgatcgtata caaataagca tcttccaaat
     2041 cttgaaaata tttataaaaa gcaccgcgcg acatccccat cttgtcaacg atttctgaga
     2101 ctttgacttg gctgatgtgc tgtgagcaaa atatctccag taataggtca tcgattctct
     2161 gtttctttgc gttcgataaa ttgaaaaaag tttcttttgg catccacatc tctcctttat
     2221 ctctgattat acgtggatag cccttcattg tcatgaagga ttcattcctt ctttttctct
     2281 tcttctgtca agtacccatc ttccattcga taaacgcgat cactccattg aatcatacgg
     2341 acatcatgag taaccataat agtcgctttt tgtttctcat gagcttcttt aactagtaat
     2401 ttgacgactt catatgcatg atctgtatcc aaactagctg ttggttcgtc tgctaagatc
     2461 aagcttggct cattatataa agcgcgagca attgccactc tctgtctttc tccaccagaa
     2521 aggtctttag gataactatt tttaagtgac tggatatcta aagattgcag caaggaatcg
     2581 atttttgttg aatcttcttt ttttgaagta acttggtcaa ctagttcaaa ttgttccttt
     2641 accgttaaaa aaggaatcaa attagagctt tggagaataa atcctatttc tttaaagcgt
     2701 aaatctgctc gttttttttc tggaagctca gttagtgagt gaccgttgat cactatttct
     2761 ccggaagtcg gtgtttgcaa tcctcctgaa atggtaagga aagtactttt acctgaacca
     2821 gaaggaccaa tgatgctgac gaattctcct tcctttacat aaaaatcaat tgctttcaaa
     2881 gctgagacct ttgtatgacc agaaccaaat gttttggtaa cttttttcat cgttaatatt
     2941 tttttcattt ttattaacct cctattgctg taattgggtc tactttggtt atcgtacgta
     3001 tcgaaaacaa tccgcctagc attgcgacaa tgatcaaaat cccactatat agcaaccatt
     3061 gtgaccaaaa aacagcaaaa ggcatcgcgc ttggtaaaat cagactagtc aaataagcaa
     3121 acaaaagagc aagcagaacg cctaccaccc caacaataaa ggactgtgct actaaagatt
     3181 tagcgatgaa gaaatttttg atcccttgtg ctttcattac tccaaaaata gcagtcttct
     3241 gaagtgtcat cacgtacatg aaaataccga ccacggcagc aaccactaag aacaaaaagt
     3301 atatcatcgc atttaaggtc atattttggg cagaatagcc aggaatactt tcaatgaatg
     3361 tcccaatagt taataactgt agacccttcg atgctttttc tttcgaaatt ttagctgaat
     3421 cctttattac gatcccattg atcggctgtt cttcttcaga agcaaacggc tgttgtccat
     3481 acttcaaagc agtccacgta tctagggacg catagatgac gggagaaact gtgtaataag
     3541 tttcaggaaa gataccgaca atcgtcaaag gatcgtcgta actgccaatt ttgatcgtat
     3601 ctccaatttt atagcctgaa tcagccagat tttgagagat gatgatttca ttctttgttt
     3661 taaattcatg ccctttcgtg agttttggta ataaaaatgc ttctttcgtt gtgccgaaaa
     3721 ctgtaacatt ttctttgttt tttcctttga ttgctccgct gtacaatccg attggtgctt
     3781 tctctcctcc ttcaatgtat ttcagatctc ctcttgttaa ttgtgaagct gcaaatactt
     3841 gatttgcatc ctccgacaaa acaatctctt gtgcgtccca atcatcgatt gcttttttaa
     3901 actcttctga aagtccattc gctagtcctg acaacatgaa tacgacatac gcaatcaaga
     3961 acataatacc gacgatcaag ccatatcgta atttagaata gcgaatttct ttgatagcta
     4021 gaaacatttg tttttcctcc ctatgataga ttgatagtga caaaatgtca cttgttgaat
     4081 agtgtaactc taaagtgaca atttgtcact tgtgttgttt ttttcttatc aataaattgt
     4141 ctaagacatc tttcgataaa accttaacat gagtcataaa aaatttattt aagccttatt
     4201 tatgctttcc atgataaact aaagtgacca aatcaatgag gtgggaaaat gattaaacta
     4261 ttagtagtag aagacgataa agtattatcc aataatataa aagaaatact cgctgcaatc
     4321 ggcgagataa cacaagttta tgatggtagt gaaggactgt tcgagttgga aagtggagtt
     4381 tatgatcttg caatcttgga tttgatgatg ccggaaatga atggatatga agtgttagca
     4441 aaaatacgga aagaaaaaat acagactcca gtattgattt tgactgcaaa agatagtttg
     4501 gaagataaga ttgaaggatt tgaacgaggt gcagatgatt atctaacaaa acctttctat
     4561 cgagaagaac tgatcatgcg cgtgaaagca ttattgaaac gctcgctagg tctctttgat
     4621 gaaaatgttc tgaagtataa ggatatcagt tgtgatttaa aaagcaatga agtcttgtat
     4681 aaagaacaag ttttgcccat acaaggaaaa gagtttgaat tgctcgttta ttttttacaa
     4741 aataagggaa tcattttgac aaaagagcag atatttgacc ggatctgggg atttgattct
     4801 gatacgacga ttacagttat tgaagtttat atgagcaact tacgaaaaca tctgcgaagt
     4861 actggattgg ataaagaaat caaaacctta cgtaatgtcg gttatatact gcaaagggtg
     4921 tgatgcaatg aatggagata ttacacgaaa gcaacgtagt cgcttttttg cgacaaatgt
     4981 agcagcattt gcttttatct tttttctttt gggattcatt actttacaga tattaaacca
     5041 atctgcatac agagaaacag atctgtcatt agagaaaata aattttgatt ctcgaacagt
     5101 acaattagag atacaaagat atcagcaagg cgatcccttt ttaaacccga ctcaaacgga
     5161 acaaaacaga acaaacatgg aacagccatt aatggaacca tcaaatgacc gttttaacac
     5221 tcaagtgatc ttgtggtcta aaaatggtga gatattgaat aaagcagcta ttggtgggcg
     5281 tttcaaccaa atcaaagaat taaaaatgga tacgaaaaat ctgaatacca tccaaaatat
     5341 tgaattaaat gaagacagtg gctcatctgc cctctctttc cgttcgatta cgaaaaaagc
     5401 ggagacgaat caagatggcg ttgcctacat ccaaatttta gaaaatacaa atcaaatcaa
     5461 gaattcatta gcgacttttc gcacgattat gattttgtgt atgattgttt tttggattct
     5521 ctctattggt atcagttatt acttgtctag tctaagcatg cgccctatta ttctttcttg
     5581 gagaagacaa aaagaatttg ttgagaatgc ttctcatgaa ttgcgcacac cgttaacgat
     5641 tattcaaaat agtttggaac atctttttac caagcctaat catactatta ttgaagaatc
     5701 cgaaagcatc gctcaagcat tgtcagagac gaggcgcctc actggattga cttcggattt
     5761 gctgacaatc gctcggaatg attctgatca gcagctgctt tcaaagcaaa tgatcaatac
     5821 acaagaatat atcgaacaac ttgttaaacc atttcaagag atggccatca tagatggaaa
     5881 agagtttatt ctccataacg atgcaacatt agaagtacca attgatgaaa aaaaaatcca
     5941 tcaagttttg gttattttgc tggataatgc attaaaatat acgaatccag aagataagat
     6001 cattttggat tcatctgtta accataaaga atggctgatt cacgtaaaga atactggccc
     6061 ttctatcagt gaagaagatc aagcaaggat ttttgatcga ttttatcggg aagatcaatc
     6121 tcgggcgaaa gagactggcg gatacgggct tggcttggca attgcaaaac aggtaatcga
     6181 acagcatcat ggacagattt ctgtatccaa ttatcttcca caaggagtcg attttaaagt
     6241 ccaattgccg ttgaagccta aaaaataatg cttatcccta aaaaagggct gtgacaaaaa
     6301 tcttgtcaca gccctttttt gttgcgaata aacggtgttc cagaagtagc ttcttcggaa
     6361 ataagccgaa atttctttaa gctgaccact tctgtcacaa cctctttagc ttacgcttac
     6421 gattttttat tgcggtcttc aatgtgtaca tcaccgcttg tgtcaatatc caattcttct
     6481 cggctaacat cttctgtcac tttttcaaca tcttcatgtt cttctttatg aatcccaact
     6541 tcttcacgaa taactgtatg tttgcttaca tctacttgct cttctttgat tgggatagta
     6601 atcgtgtcat cttccaattt ttcgtctgtt ccctgtgaag attgatctgt tactggtttg
     6661 cgttcgatga cgatttcttc acgtttgacc ggtacttcta ccgtttcagt atcgttgacc
     6721 acatgtttgt ggatgtcgac ttctccagtt gtcacatcat gtgtatttac atctaatttt
     6781 tcttctttta gttgaattgt atcatcatct agatttggtt tgcgatcgat gtcttcagtc
     6841 ttgcttgtac ccattgtatc agtctcgcca ggtacgtcca tcggatcagt tcctggtgtc
     6901 atattcaatg gatccgttgt attttgaaag cctgtcgtgc cacctacgtc cattggatct
     6961 gttccagtgc cagtttctaa tccagtcccc attgtgcttg ctgggctaac agtatcttgt
     7021 gtatccattg tatcttctgt tgtttctggc tgacgatagc cttttactgt gatgacaaat
     7081 tttccatcag aaatatctcc tctatattga tataaaggat cattttctgg tgtagccgtc
     7141 tcagaatcat aggtatcaaa agaaaaagta tcttttattt tttcccataa agaacggtct
     7201 tcttcaccat ccatccgatt ttcatctgtt tcaacatcta taccagacaa tccgtcaaat
     7261 cctagtctag atgctgcttc atcagtagta taaataacga tatcatctcg ttcataccct
     7321 tcgttacgca accgttcaat tactgataat gcttcttccc tcgtactata gcttccgatg
     7381 accgttaaat ttttctcttc catgtaaatt cctcctagca aatagattta tgattggtat
     7441 attgttactt ataaatttat tctataggag acgattgttt tct
//
LOCUS       DAHHZJ010000138         7382 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.140, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000138 DAHHZJ010000000
VERSION     DAHHZJ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.140"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(<1..1152)
                     /locus_tag="NBL02_002274"
     CDS             complement(<1..1152)
                     /locus_tag="NBL02_002274"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HCD2572948.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            1337..3778
                     /locus_tag="NBL02_002275"
     CDS             1337..3778
                     /locus_tag="NBL02_002275"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HCD2572949.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(4108..4812)
                     /locus_tag="NBL02_002276"
     CDS             complement(4108..4812)
                     /locus_tag="NBL02_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572950.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(4999..5244)
                     /locus_tag="NBL02_002277"
     CDS             complement(4999..5244)
                     /locus_tag="NBL02_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572951.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(5261..6112)
                     /locus_tag="NBL02_002278"
     CDS             complement(5261..6112)
                     /locus_tag="NBL02_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCD2572952.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            6278..7282
                     /locus_tag="NBL02_002279"
     CDS             6278..7282
                     /locus_tag="NBL02_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HCD2572953.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         2328 a         1325 c         1527 g         2202 t
ORIGIN      
        1 tattttgtag taacgaatat cattttcgat ctttgtaatc gaaaaaggta catctgaagt
       61 atattctgca cattctccgt gttcaggaag cgtctcactg aaaatcgttt catattcgtc
      121 aacagtgatc cgtgttcgag aatctagcat ctcttgatga gattgtgcga agagatattc
      181 ttgatagttt tcttccagat aacctgtaaa aaattcactt actgcaccag aaccgtagct
      241 gaatagaccg atgcgatctc ctggctgtag tgatttcgaa ttttccagta gagaagttag
      301 ccccaggtat aatgaaccag tgtaaagatt accgattcgt cggctgtaac ggatgctttc
      361 ttcatagcga gccataagac gttcctgatt ttcttcgtct gtttggtcta atacgctttg
      421 caatgccttt tttcccatct tagtatacgg aatatggaaa gcaatcgcct gataatcttc
      481 gagtcctcga cccgacaatt ctttatgtcg attccaaact ttttggaatg attcgatata
      541 cgtagaatta gataaaggac catcaacaac aggaaattcg ctataatctg gacgccagaa
      601 atcatagata tcttctgtca gaaatacgct gtcgtcttca atcgataaaa tacgcgggtt
      661 ttgagtgatc atcatcgcaa cagcaccgac accttgcgtc acttcaccac cgcttgccaa
      721 gccgtaacga gcaatatcac ttgctatgac tagtactttt cgttctggat gatttttgac
      781 atattctttc gccatgtgca aagctgcggt gccaccgtag caagcctctt ttacttcaaa
      841 agaacgagca aaagattgga ttttcagtaa atggtggata attaccgcac tcgcttttga
      901 atgatcaatc ccggattctg ttccgacgat gaccatgtcg attagctcac ggtctttttc
      961 tgttacgatt ttgctggcag catttgctcc tagtgtaatg atgtcttcat ttgcacgatt
     1021 gactgccatt tggtcttgac caatccctat atggtatttt gcaggatcat ccccacggct
     1081 ttctgccaac tctgtcatat ctaaatataa attaggaata aaaaaggaaa gacgatcaat
     1141 ccctattttc atttcaacac ctctttactt atgataaaaa acttacttta ctaaggtatc
     1201 ataaaaagat taaaagaaat ataaaaaaga gaatatttaa ggattgttaa aatttaatct
     1261 tagaaaaaaa taactggaac aagagctatt tgtttttaca gcaaagaatg gtatattttt
     1321 gtaggaggtg gcttacttga aagaagtcgt tatgatagat gctgcaagaa cgccgatagg
     1381 aaagtatcgc ggaagtttaa gtccatttac tgcagttgaa ttaggcacac ttgtgactaa
     1441 aggattatta gataaaacaa aactgaaaaa agataaaatc gatcaagtaa tatttggaaa
     1501 tgtcctgcaa gctggtaatg gtcaaaatgt tgccagacag attgctttga acagcggtct
     1561 ccctgtagat gtcccagcaa tgacgatcaa tgaagtttgc ggatctggta tgaaagcagt
     1621 gatcttagca cgccagctga tccaattagg tgaagcagaa cttgtgatag ccggcggaac
     1681 agagagtatg tctcaagcac ctatgctgaa acagtatcag tcagaaacaa atgaatatgg
     1741 tgaaccaatt tccagtatgg tcaacgacgg attgactgac gcattttcaa atgcacatat
     1801 gggattaacc gcagagaatg ttgcaacaca attttctgtg agcagagaag agcaggatcg
     1861 ctatgccttg tcgtcccagt tgaaagcagc acatgctgtc gaagccggtg tattttctga
     1921 ggagatcatc ccagtcaaga tttctgatga agacgtgtta tctgaggatg aagcagttcg
     1981 tggaaatagt acattggaaa aactgggcac gttacgtaca gtattctcag aagaaggaac
     2041 tgtaacagca ggaaatgctt ccccgttgaa tgacggtgcc tctgtggtga tccttgcatc
     2101 caaagaatac gcagaaaata ataatctgcc ttatttagca accatcaaag aagtagcgga
     2161 ggtcggtatt gacccaagta tcatgggaat cgctccaatc aaagccattc aaaaattgac
     2221 agatagatca ggaatgaatt tatcaacaat cgatcttttt gagatcaatg aagcatttgc
     2281 agcttcttct atcgtagtta gccaagaact gcagttagat gaagaaaagg tcaatatata
     2341 tggaggagca atcgcattag gccatccaat tggtgcaagc ggtgcacgta tcttgactac
     2401 attagcttac ggattgcttc gtgaacagaa gagatacggt attgcttcat tatgtattgg
     2461 tggaggttta ggcttggcag tcttgttaga agcgaatatg gagcaaacac ataaggatgt
     2521 gcaaaaaaaa aagttttatc agcttacgcc atctgaacgg cgcagtcaat taatagaaaa
     2581 aaatgtattg acacaagaaa ctgcgttgat ttttcaggaa caaacacttt ctgaagagct
     2641 ctccgatcac atgatcgaaa accaagtgag tgaagtggaa atcccaatgg ggatcgcgca
     2701 gaattttcaa atcaatggca agaaaaaatg gattccgatg gcaacagagg aaccatctgt
     2761 cattgcagct gcaagtaatg gtgcaaaaat ctgtggaaat atctgcgcag aaacacctca
     2821 gcggctgatg cgagggcaaa tcgttctatc aggaaaatcg gagtatcaag cagtgatcaa
     2881 tgcagttaat catcgaaaag aagagctgat tctttgtgca aatgaaagtt atccttcaat
     2941 cgtgaaacgc gggggcggtg ttcaagatat ctctactcgg gaattcatgg ggagtttcca
     3001 tgcatactta tctattgatt ttttagtgga tgtaaaagat gcaatgggcg ctaacatgat
     3061 caactcgatt cttgagagtg tggcaaacaa gttacgggag tggttccctg aagaagagat
     3121 tctattcagt attttaagta attttgctac agaatcattg gcttctgcct gttgtgaaat
     3181 cccattcgaa cgcttaggga gaaacaaaga aatcggtgaa caaattgcca aaaaaatcca
     3241 acaggcaggg gaatatgcga agcttgaccc ttaccgtgca gcaacgcaca acaaagggat
     3301 catgaatggt atagaagctg tagtggcagc tacaggaaat gatacacgtg cggttagcgc
     3361 ttcgatccac gcctacgcgg caagaaatgg attataccaa ggattaacag actggcagat
     3421 caaaggggat aaactagttg gaaaactaac ggtgcctttg gctgttgcca cagtaggcgg
     3481 agcttccaat atcctgccaa aagcaaaagc atctttagct atgttagata ttgattcagc
     3541 caaggaacta gcacaagtaa ttgctgcagt agggttagct caaaacctgg ctgctttgcg
     3601 tgcattagtt acagaaggaa tccaaaaagg ccacatggga cttcaagctc gttcattagc
     3661 gatctcaatt ggggctattg gtgaggaaat cgagcaagtt gcgaaaaaat tacgtgaagc
     3721 agaaaagatg aaccagcaga cagctataca aatattggaa aaaatccggg aaaaataaaa
     3781 aagcaatatc tttagaagaa taaagcaaga tttacagtta ttttatttac tgcagatctt
     3841 gcttttttct ctatcagaaa atatgtttat tattagaatt ttttatagtt aaggttaggc
     3901 atcttaagaa atcaggcaac tccaagatat cttcaggtag aagagaagtc gcctaacatt
     3961 gtaggatcat aggtgatttg ttggacaggt caagctgata tttgagcctg atgttagaag
     4021 gaatttcttt ttacttgctg tagaaattat tatatgacta aaaaggctgg gattttaaat
     4081 cccagccttt tatgacaaat agaattttta tcgatcagta atcgctgtcg ctgcaaattg
     4141 ttcgatcaaa ggaatcaaag tcaatttgac tttgtatttc cgttcgagtg ctttttcgat
     4201 aacgaactca ttttcagtag tcatagaaat agttgctaat ttacgaggtt catcagcatt
     4261 tttttcatta ataactactt catgttcatt ttgttctgtg taaaatttag gatactcagc
     4321 aacggctttt gaaaaatcag caaaacttaa agcggcattc gtatcagttg agcgtgtatt
     4381 ttcagttgtg tccgcttttg gtttttccgt tttttcttct ggcacactga ctgtttcttc
     4441 tgtaactggt gtcgcaacag cttgcgcagt tggttcatcg tttttcaatc gttgatcatt
     4501 ccagtaagac acgatttcaa taagaagatt gtcacggaat cgttcgaaaa tcccgttttc
     4561 tcctgaacga attctcttta atacagtatt gaagttgtca tcgattggca cgttgatccg
     4621 tgtatcttca tttgttttcc cagctaatcc gatcgttaag atttgacggt ttttgggatc
     4681 aagcatgact tgtaacggca aaccataacg tcgcaagctc tcttcttcta gagaacggaa
     4741 taattgtcct cttacaccat ccaatgaagg acggaattga tttaataatt tttgtttctg
     4801 ttttttcttc atatgttctc tccttcgctc ataccctttc gattataaga aaaaaacagc
     4861 caaatgaaaa ggagtaatgc aaaaaaactt gcgtatacgc aaattacaac agaaaaattt
     4921 gcgtattttc ccgttttttg aaagaaaaat aaaaaatgga acggattatg tatatataca
     4981 taatccgttc cattctttct attttttcgg tatcaaggca aaaacaggta gttcaggtga
     5041 ataaatgact tcatgttcgt gccagggttt gaccattagc gaatgatcga accgtttttt
     5101 cagttcttcg atgatttgat catgtgtcca gtttcttgca ggaaaatgtt ggattttctc
     5161 tgcatcgatc aaaaagacaa aatcggaatg aaaagtctgg gtaacttctt ccgagagaga
     5221 attcaagcgt tcgattggat ccataaaatc acctttcgac ttaaaattgg gtagtatctg
     5281 ggtcttttgc tttgccgact acaccatcta actgatcgat ttgtttcatt tcttcttctg
     5341 ttaattcaaa atcaaataat tcagtatttt ctttgattct gcttggtgtc actgacttag
     5401 gaagaggaag gaattcgtgt tgcagcgacc aacgcaaagc aacttgtgca atcgtcttgt
     5461 catgtgcttc agcgatttgt ttcatttcag gcacatcgaa gattttacca gtgcctaatg
     5521 gactatatgc ttcgaggatc atatcatgtt tttttgcata ctcaactaca gttgtctgta
     5581 attcgcctgg tgcaaggaaa acttgattga ccatcggcat aatttttgct gtttttgcta
     5641 gtgtatctag atggtggggt aagaaattgc tcacaccgat tgctttgatt ttacctgctt
     5701 catacagttc ttcaaatgct cgccatgtat ctgcatttgc ttgttcccaa ttatcacgaa
     5761 aagcaacagg gtttggccag tggatcaaaa acagatctaa ataatctgtt tggagcttct
     5821 tcaatgattc ttcaaaagaa gacatcacaa gttcatagct gtggtttgca ttccataatt
     5881 ttgttgtcaa gaatatctct tctctaggaa ttccactttc cttaatcgct tggccgacgc
     5941 tttcttcatt cttgtatcct tgggcagtat cgatatggcg ataacctgca gccagcgctt
     6001 ctttaacaga ggaaacagcg acatccccat caggtgtttg ccatgtgccg aatcctacta
     6061 ccgggatttc gtaaccattt gatagacgat aagtgcttgt taatgaattc atactttcag
     6121 ctcctttttt attcgttcaa ctataagtgt agcgcatttt tcgaaaaaaa gaatccattt
     6181 tgtttctact cttgaaattt ttgtttaaaa gaaaatgatt ttgattctgg tatttttgat
     6241 tttttttccg taaactaaga cagaagggaa tgagaaaatg gatatctatt taaaaaaagc
     6301 aattctgcat attattgaca gagaaactgg ttctccagtc ttttcacaaa aagagctgga
     6361 tctgacaaaa gaatatattc gtgattttct tcaaaagaag atccaaaaaa tctcttctgc
     6421 tcaaacaaaa actggacaac tagcagaaga aagcatgatc gttgaatcac ttaaaatcgc
     6481 tcaagaaaat tttgtggaag caagtgaagc aatcgttcag cggtggttcg atatctacca
     6541 agaaagtgaa gaagcaccta gtgcagatgt atttgttgtc ttgtatgaat tagatacagt
     6601 gatgtactta tctttattga aagtcaatta tcgtgaggct tacactcact ttgttgaagc
     6661 agacgaagca gggatcgaca accgcttgat tttgaatcga gcaattctag gggcgaaaac
     6721 acaaaaagca gatgaagcat tcgctgtgaa tttgactgat tggacctacg aattaattga
     6781 aaaaaaatat gagttttctg gcaaaaaaga gctttatttt tctggaagag tgatcgaaag
     6841 tgttcctgca ccttctttag aagaaaatgt aaaagtgatc caaaaagtag ccaagcatct
     6901 tggaaaaaaa tttgagacag aagaatttga tatcatggct gatttgaaag aagctgtcta
     6961 tgatacgata gaagaaaaag gtcggttgga tcatgagatg attgcggaaa aagtcttcaa
     7021 ggaaaacatc acggcaaaaa tggcttttaa agaagaagta caagagcaag gatttgtccc
     7081 agaagcacct cctgtaaaag aagtacaaga aatttctgag aaaaaatttg gaaaacaaaa
     7141 attcaagcta tcaaacggga tcgaactgat cgtaccagta gatgtatatc gcaacccaga
     7201 tttgattgaa tttgtgaata atccggatgg tacgatatcc gtgatgatca aaaatgtgga
     7261 tgaagtattg aatcgtttat aaaaatgact agaatattag gtgttcaagt accagtgatc
     7321 agttaaatga attgtgtcaa gagaaggatc ggaaaagacg aatataacaa aaaaagagcg
     7381 tg
//
LOCUS       DAHHZJ010000139         7119 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.141, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000139 DAHHZJ010000000
VERSION     DAHHZJ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7119
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.141"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(<1..219)
                     /locus_tag="NBL02_002280"
     CDS             complement(<1..219)
                     /locus_tag="NBL02_002280"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCD2572954.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPI"
     gene            complement(330..584)
                     /locus_tag="NBL02_002281"
     CDS             complement(330..584)
                     /locus_tag="NBL02_002281"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCD2572955.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(777..1052)
                     /locus_tag="NBL02_002282"
     CDS             complement(777..1052)
                     /locus_tag="NBL02_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HCD2572956.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1024..1977)
                     /locus_tag="NBL02_002283"
     CDS             complement(1024..1977)
                     /locus_tag="NBL02_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCD2572957.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2589..4082
                     /locus_tag="NBL02_002284"
     CDS             2589..4082
                     /locus_tag="NBL02_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HCD2572958.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4217..4513
                     /locus_tag="NBL02_002285"
     CDS             4217..4513
                     /locus_tag="NBL02_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HCD2572959.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            complement(4537..5496)
                     /locus_tag="NBL02_002286"
     CDS             complement(4537..5496)
                     /locus_tag="NBL02_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCD2572960.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHFLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIR
                     CSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFK
                     SITFDCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDESFIQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
     gene            5679..6530
                     /locus_tag="NBL02_002287"
     CDS             5679..6530
                     /locus_tag="NBL02_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572961.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            6551..6834
                     /locus_tag="NBL02_002288"
                     /pseudo
     CDS             6551..6834
                     /locus_tag="NBL02_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2363 a         1150 c         1286 g         2320 t
ORIGIN      
        1 gatcggttta ttagaccttt gaggtctata ttttttaact acaatcgacc ttaaatttaa
       61 ttgtttcatt agcttttgga catgtttaag agatactgaa atcccttctt ttaataaaac
      121 ttgatggatc tttgtggcac catagcgttg tttattcgta aaaaaaattg ttgaaatctt
      181 ctttttcaac ttattatttc tgatttctgt agctgtcaac ggtttattct tataaaaata
      241 atagacactt ctagggattt ctaataaccg acacaacaaa gaaacagtgt aatccttaca
      301 ccatttttca ataaattgaa caagcgaatt taggttttcc gagagaatat ggctgcggct
      361 ttttttaaga tttcaagttc ctcatttaaa cgagcatttt cttttctcag ttcagctact
      421 tcttttccag tcagtcctgt ggactgatca ggtaaatata aattcttcca tttgtaaatc
      481 gttgcttcag ataagccata ttctttcgct aaccctcgaa cagaacgtcc agtttgattc
      541 agttcaacaa tcatttgttt aaaattttct tcatatcggg tcataaaaat tcatcctctc
      601 gtagataagt ttaatttatt cttgtctcta cgaaaagctg tctaagattt gatactaaca
      661 ccaaatgttg tataccccca aacaatacac atattttgaa ggattaggat taataaagct
      721 aaaaatatag taagtatact aaatatattt actatatcta gtatacttac tatattttaa
      781 tcttttgatc gtctattata tataatccgt tcctcgtcgt ttaaagtatc aacataggcg
      841 tccattgcaa gtttgactat ttcatacata ggaatatctt ttgcataact aagatcccga
      901 atggttttta gtacgggagg atctacttga actggttttc tttctttcgc agtgtacttt
      961 cgttccccta actcgacatt aatatcatct ttaattgaaa tatcttcagc accaattttc
     1021 ttcttatctt tgttcgttgg taataatgcc atttagttta atctcctttc caagaggtag
     1081 gatatgattg gccattgagt cagcatattt aggtgtataa tcaaatcctt caatatcacc
     1141 atgttgttca tagtattcaa ttctttcttc taattctgta aagatgtctg caaaaatagc
     1201 ccaccatctt cgatctacat aatcatttaa ttggatacca ttctcaccat acatttctaa
     1261 ccgatccgag cctttaacaa tgcttttaaa aatgtttgat tcaccatact tatcaattgt
     1321 tttttgatag tttgcgtgtt gagaggaacg tcgcactgag aaaagaacag gcaaaattcc
     1381 aatgatttga agatgagact cttcaaaatt cgtgacaatc ggaactaaaa ctttattaat
     1441 aaaatcttct gttccttcca ttgcgtaccg ttttaattct tgcattgcaa taatataatc
     1501 tgcagcagtt aagaatgcac gaaccacatt atctgttgat ggtgggcagt cgataaagat
     1561 ataatcgaaa ttttttctca attcttctaa aggtttaatg aaggccaatg atctcttacg
     1621 acgatcagga tatttttcgg acaagtattc attaagttct tcagatccag gagagccagc
     1681 cataatatat aaatgtggac ttacttgagt aatacctgaa acccagtccc cttttttgat
     1741 tgctgcagac atggatcgat caaagctagt aatttgatac gttgatccaa tgttggaagt
     1801 agtatttctt tgttggtcat aatcaattag caacacgtct ttttcaaaaa gttgactaga
     1861 aacaatcgct aataaatcat tgtctgtagt cttaccaatg ccgccttttc tacctgtaag
     1921 cattacaact tttcctagtt tatctgatgt tttaaatgca gtccgtaagt tttccatagt
     1981 tccctccaat ttcctatact aaatatattt agtatacttc atatacttaa tatattcgat
     2041 atatctaata tactccaaat tgataagaat tcagacttta taaggaatat agtggatata
     2101 ctaaatatat ttgctatatt cagtatatca gatatattca aaatgtcaaa atatatttgt
     2161 gcgttttgcg ttgacaattt agagtgaaaa gaggtaaact taattctata gaaaccacag
     2221 ataattcact atttacaaag tagcaaaagc agtggtactc tttaaacaga aaaattacat
     2281 aaaaaaaaga gacggaaata attgatttta gcagaatcaa ttatttctga atcacatggt
     2341 taggacatgt gttcacgctc gtctcaagaa tatgtaagta gctaccaact acttatatta
     2401 aaagttgatc ggataccttt tccgattgcc tttattctac caaaaaaagg cgagcaactc
     2461 aacagaagaa cgtttattta gtgtacttaa attaaatatt cttaaacact gaagagagaa
     2521 cacatactat ggctgagatt aaagctaaac tgtgttctct ttttgtttga gaaaggacgt
     2581 tacaatcgat gttgagcgaa atttattcag ctattcttaa aaacggctta cgtacataca
     2641 aatatcgaaa cagcaccatg aaacctgcag gtcatattga ttccagtagg agtggtgcta
     2701 tctttgcgta ccgtagcaag caattaatga atgttgggcg tggtatggtc attacttcgg
     2761 aagaagctat tttagagaac gaaaagaaac taacgcattg gacacctaat acctatcgct
     2821 ttggtactta tgcggatgat tatcgaacag tggttaaagg tcattctgag aaaaacttaa
     2881 gtcaaatcaa cacatttgta gttgatatcg attcaaaaga gaaccatcaa ggcgaaatta
     2941 ttttagcctg tttagatcaa gtgggctaca tgccaacgct aattttagaa tcagatcatg
     3001 gttatcaagt ttattttgta ctaaagacac caggctatgt cacaaagaaa agcaatttca
     3061 aggtgatcga tgtagcaaaa aaaatttcaa aaacaattcg tctacagttg gctgaaaaat
     3121 tgtccggagt ggatctagga tgtaaccact tcggaattgc tcgattccca caagaaaata
     3181 atattgtttt taaagaaatg aactatcagt attcttttgc tgactggatt caatggtcga
     3241 tgaagcttga atcagatcaa aaaacaaaag aagatcgaga acgaaaacta attgtctttc
     3301 ctgagaaaaa agaaattcgt caagtagatg aaccgtggtt cgatatgtta cttcataaag
     3361 caaacatcgt cggcggagaa ggacgtcttg gtcgaaataa tgtgattttc acgctttctt
     3421 tagcttatta ctcttcaggt tatgcattgg atacttgcga atacaatatg ttcacattca
     3481 acgaacggtt acaggaaccg ctatctgaag cagaattagt aaagattgta cgtagtgctt
     3541 attctggtaa ttatcaagca gcaagcagag aattcattat tgaattgtgc cagcaatggg
     3601 tttcaccaac cttaaaaaat aatgatctgt tcattcaacg acgtggctgg tggaaattta
     3661 aaaagccacg agatcaaaga aaatatagtc ataaaaatga atggaaagaa gatctattgg
     3721 cttatctaag cgaaaaaagt cttgctagtg agccttattt gaacctgtca aaaaaagaat
     3781 tggcttcaca actaacgatg cccaagcgaa cacttgataa gatcttaaat gagttacgag
     3841 aggagaataa agtcttctac aaggtaacac gaggaagaaa tggcggaatg atcattgctt
     3901 cagtcaaaac gttgttcgcg agggttattc aactaagtaa agaaaagaga ctagattatt
     3961 caacaaggat cacagaggta tttggactca acgagagctt agtagaagag acatttagac
     4021 aattatcaac ccccttgaac cctcttaaag aagtgaatct ttttgaagta gatacaggtt
     4081 gaaaaataaa aggtgcaaat tgccttatac aatatatcta tgatacttct gtaggtgttg
     4141 ctccctttgc ctagctacgt gtaggtggta tacttataac tgcgtatgca gtcttatgta
     4201 tcatggagga agttgtatgt cgttaaaaaa ttatgtctat tcacatcctg ttaatgtgtt
     4261 tatcattggt cgtttgggat tatctgtgga agtgttctgt gagctttatg gttttaagca
     4321 ggggactttg tcttcttggg ttaccaggga aaaaacagtt gcttctttgc cgattgagtt
     4381 tcttcatggg ttgtccttag cttctgggga gactatggat caggtatatg attgtttgtg
     4441 tgtgttagag caggagtata ttgagtataa aatagctaac gagttaagaa aaaggaagaa
     4501 gtatatccag tagtagtttt tatttactct taattgtcaa attaagttag acaaatcatc
     4561 aatacttacg taactcaaaa atacttccaa aggtgttcga taattcagtg attttctagg
     4621 aatattattt cgtttcgatg cgacagattg aataaaagat tcatctactg aattgaaatc
     4681 catagatttc aataaaccat ctctacgtaa caatccgtta gaattctcgt ttaggcctct
     4741 ttgagacggc gttcctggat cagcgaaata aatggcaata tcattggcat tactgatctg
     4801 tttccaattt gaaaattcct ttccacaatc aaaagtgatg gatttgaata gatttttcgg
     4861 tacagattca aaccaatgat ttaatttgtt ttcaatatca atcgcttgtc taccacacgg
     4921 tttcaatgtg atgataactt ttgataatcg ctcaactaag gtaattacag cacttttatg
     4981 tttcaaacct acgatagtgt caccttcaag gtgaccaaac tcatttgaga attggctata
     5041 atctttttca cgttcatgaa tagagcgacg gaaagcttgt tttccacgtc tttcttgatg
     5101 tccatttggt ttacgcttgc ctttcatcgg taagtcagaa ggattaaata gccccttttt
     5161 gaacatacga taaatggtac gagcagaaca acgaatagga aacgctgcac gaccaacaat
     5221 cacatcgggt gtccatcctt gaacaacctt tctttgaata tattctgatt gttcctcagg
     5281 taaaacaagc gggcgtctac cacagtttga tttgtttttc ttatactgtt gatagtactc
     5341 taaggctgat tttccttgct tgaagaacag gtatacttta tggatcgttt gtcttgaacg
     5401 attcaagaaa tgcgcagttt tcgcaacaga ttgatttatt ttgaaataag actctatcat
     5461 tacaagttca tccgttgtaa gatgggtata ggtcatttgt actcactcct tataattctt
     5521 tggacggaac tattttgagt gtagcacaaa tgactttttt agttgtctag cttaatttta
     5581 caatcggcgt acttaaaaaa ttggttcatt gagcaaccta tcgctgctaa agtagcaaag
     5641 gaaaacgtta aaggaaagaa tttagggaag aaatcgaaat gaaatcggac gactggattg
     5701 aacacacctg gcttcctctg aaaagaaata gcaaacgaaa gaaattttgg gtattgcaat
     5761 tatggccatt aggaaaactt tgtagttacg gggcacgact tattcttgat ttaaccgttt
     5821 actcgattgg agtatttgga acggcttatt acttgataaa tgatcccaat aggattgcag
     5881 catcagacat ttttacagtg ctaatgacgt tgatttttgt catgctattg tctattttta
     5941 ctcaattagt catgcaattt accaggcaaa aattaacagc aggaaaactt ttcaaaagcc
     6001 tattcgattt aattgttgta aatgcaattc tagcgacttt aataaaacaa attgaacaat
     6061 ctggaagaac aacaaaagga gtgaaggatt ttttattgac gtttattcac tttgtaaatg
     6121 gacacatgta tttgtttgaa gtgggtatta ttctcttttt tgtcgggtat gtatccacga
     6181 aacttattaa acgttcaatt gaaaaagaaa ttgaaattga cctgattccg acacttcttg
     6241 gttatgagta caatgttgat tttggaagat atgacgaatt gatcaatacg gatatcgttc
     6301 acacgagtat ccatgaatct actgttaagg gcatcagatc tattttaaaa aatggccagg
     6361 aaatgaaagc aatagatcga agaaaagaga tctacaattt ttatgaagtc gaagcaagcc
     6421 tacgatatgg catcggaaaa ttgtggttag atggaagtaa tattcgtcaa aacaaaccat
     6481 acattttttg gaacagtagt gatttagaac ttccaatgaa agataaataa ttatggagaa
     6541 tcgtttgaaa atgattcctc tatacaggat tgtgcttata gttggcatgg agttaaaaac
     6601 cttaaagaag gagcagttat tcaaagtgaa agaacgtcat aatcagttac tatgtagtaa
     6661 aacgggtacg atgaacattg aataccgaac agaatattta gaattaacga atcaaactga
     6721 ttttttgttg attcgaaagc aacaagagca agaaaaaaat taatggctac ttatagccaa
     6781 ttgattgatg aatgggaaca attcaaggaa tctaaaattc cagaacgtct ctaaaagcct
     6841 ttatttgtaa agagcataag gaaacgtggg aacacttttt aaaaaaagtg ttccaatagg
     6901 ctgtgcctat tacgtttctc aaaaataaat gtgggaacaa aatatttaaa aataggatag
     6961 tgaaaggata gtgaaggata gtaaaaggag ggtagtagga tagcaaaaag gataggctcc
     7021 ttttcgctat cctttttaaa ttggaggaat taatatggaa gtaagaataa gaaatgtgga
     7081 tgagaaaatc gtaagagaaa ttgatcgaag atgtgacga
//
LOCUS       DAHHZJ010000140         7102 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.142, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000140 DAHHZJ010000000
VERSION     DAHHZJ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.142"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(581..1597)
                     /gene="tsaD"
                     /locus_tag="NBL02_002289"
     CDS             complement(581..1597)
                     /gene="tsaD"
                     /locus_tag="NBL02_002289"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCD2572962.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(1594..2058)
                     /gene="rimI"
                     /locus_tag="NBL02_002290"
     CDS             complement(1594..2058)
                     /gene="rimI"
                     /locus_tag="NBL02_002290"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCD2572963.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2065..2607)
                     /gene="rimI"
                     /locus_tag="NBL02_002291"
     CDS             complement(2065..2607)
                     /gene="rimI"
                     /locus_tag="NBL02_002291"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCD2572964.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(2591..3313)
                     /gene="tsaB"
                     /locus_tag="NBL02_002292"
     CDS             complement(2591..3313)
                     /gene="tsaB"
                     /locus_tag="NBL02_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCD2572965.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(3504..4484)
                     /locus_tag="NBL02_002293"
     CDS             complement(3504..4484)
                     /locus_tag="NBL02_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCD2572966.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(4508..5992)
                     /gene="galT"
                     /locus_tag="NBL02_002294"
     CDS             complement(4508..5992)
                     /gene="galT"
                     /locus_tag="NBL02_002294"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HCD2572967.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6004..6993)
                     /gene="galE"
                     /locus_tag="NBL02_002295"
     CDS             complement(6004..6993)
                     /gene="galE"
                     /locus_tag="NBL02_002295"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCD2572968.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         1935 a         1553 c         1259 g         2355 t
ORIGIN      
        1 taaatatcag ggtcactgct tattttcctc cctaaaactt ctgaaagatc cggcagtttt
       61 agatcactgg acaagactca aagattttct atgtgtgttt cagttatgta atattcactg
      121 tttgcaaaaa atactgttag attaactaga tgaagcgtct tgtcctcatc cgatctagat
      181 tattcgatat tcctcgacaa taaacgatta ctcagtgagg gcaatcagag gaacggataa
      241 tgataaataa gctgtaaaaa atagcaaaat aacaataatg attaaaagta tgtaaaaaat
      301 agggctttga caaaaacgtg tcacagccct atttcgatta aacggtgtgt acctgtcaac
      361 tcttcggtca caagtcataa tgttcataaa catgaggagc tgttttttga aattgttcct
      421 tgtgaatcag agttaaacga caggcttacg aactcttgat taaacggcgt tcaaaagctt
      481 gcatacgcgg taataagaac agccaggtca aaaacatgag aagccgtttt tcctgggctg
      541 ttcttattac ttgatgcagg gcagcttttt cacaacctct ttattcagat aaaaccaagc
      601 ttggatcagc attcaactga tagctagcaa aatttctttt ttgcatttcc acatgtgctg
      661 ctgctccaat cattgcagca ttatctccac aaagtcgaag agggggaatc actagttcta
      721 cctcagggag tttagttgcc aaagcatcct gcaatccttc acgcaaccct tgattggccg
      781 ctacacctcc agctataatc agttgcttga ctggataatt ttgacaagct cgaagtgttt
      841 tagtaatcaa tacatcgatc acacttgctt gaaagctagc tgctaaatca tttttgtcta
      901 gatcttctcc acgctgttgt gcattatgca ccaaattgat aaatgcactt tttaatccgc
      961 taaaactgaa atcataatta tcttcatgga tcatcgctcg cggaaaatgg taattatctt
     1021 ttccctgatg agctagctga tcgatttctt ttccgcttgg gtaagaaaga cctaatactc
     1081 ttccaacttt gtcataagct tctcctgctg cgtcatctct tgtctcccca atgatttcat
     1141 atgatccatc ttcttgcata tagactaatt ctgtatgtcc tccgctaaca agaagtgcca
     1201 tcaatggaaa ctggaatggt ttaaccagac gggctgcata gatatgccct gccatatggt
     1261 ttacaggaat aagcggcaat tggtgtgccc aagcaaaagc cttagctgca gaaataccaa
     1321 tcagcaacga gccgacaagt cctggtccat aagtgacagc gactgccgat aaatcttctg
     1381 ccgacacacc agcttctacc agtgcatctt ccaagcataa ggtgatttgt tctacgtgat
     1441 ggcggctagc tacttcaggt actacgccac caaaacgttt atgactgttt atttgagaag
     1501 caacgatgtt agataagatc tctgttccat ttctaacgac cgctacactc gtttcatcac
     1561 aacttgattc aatcgccaga atcaattctt cattcatttc atcgtctccg tttttaactt
     1621 tctgctcata atgatcgcat cttccgtcgg attgcgataa tatgatttcc gtttacctaa
     1681 tatacgaaac ttttctgatt cataaagttt tcttgctgct tcattagata gacgtacttc
     1741 taaaaaaaca tgcagcgaat cttttatttg ttccgtaatc agtaattgta acaaatgccg
     1801 tgcatgccct tttccttgtt ctgaaactgc aacagcaata tttgtgatct cgacttcatc
     1861 tattactttc gagtatccaa taaagccaca gatctggtta tcttcagcta aaagcagata
     1921 atcagagtga ggctgttgga tgtctgccaa aaactgcatt ttcgtccaag gtgaaccatg
     1981 tggataagca ttttcgctaa tcatccatac gcattctgcc aagtacgctt catcaaaatc
     2041 tttttttctg caaatcaact aatctcactc attttctaaa agttttatca tatccaacgc
     2101 atcttctttt gtttcagtgt aatatctttt tttgatcgtt gttgcttgaa accccaattt
     2161 ccgataaagt ctttgtgcat cttgattact catacgcact tctaatgaca tcgtctcaca
     2221 gcgattcatt atagcaaagt tctcgatttc gtcaataagc agcgaaccga ttcgtttacg
     2281 ctgatatgcc ggtcgaacgg caatattcgt gatatggcaa tctaaaccga acgctcttga
     2341 accgataaaa ccaatcattc ttccattatc aaagatacct agatatagat gaggaagcgg
     2401 tgacttcaat tctgatataa aggcactttt tgtccatggc aattcgccat tatagacttc
     2461 tcgttcgaca tccagcagct ctttgatatc ttctgtagtc aattctctta gtacgtaacg
     2521 acgatcgatt tgttcgactg ttttttctgt ataaatcatt ttatcttgaa aaaagcttct
     2581 ggcatacgct ctaaattttt tcaacataac tttcgtctcc aggtgtgtgc gtctccaacc
     2641 atttttcttc tgcttctact cgttttaaat agcgaggaag aaaagcttgt ttctctgcaa
     2701 ctggttcaac taaagcacct aattgagcca cagttacacc acttggaata tcccatgctg
     2761 gaactgtatt aatcatcgca ctaggcaata tttcctttat ttcagagaag aatttgtgac
     2821 aatcactccc gacgaaataa atagcttcat tcagcttttg tagttgtgtg aacaaatcag
     2881 aagcagaaat atgtacatct tcaagtacag tttctaatct attttctttc cagcggtatg
     2941 ccccagcgta tacatttttt cttcgagcat caaacatggg cacaataata ttatccacac
     3001 cgatgcaatt agccgcgatg gttttcagac tagatactcc tactagctct ttatctaatg
     3061 tataagcaat cgtttttgct gtcgttacac ctatacgaag ccctgtataa gaaccgggac
     3121 catcagaaac cgcgattcgg tcaatgtctt ttggacttat ttgaagatct gcaaacaatt
     3181 ggtcgattgc aggcatcagt gttacactgt ggttgcgttt gatatttgtt tgtatttgtc
     3241 caagaatttg ttcgtcttct accacaccga tcgccaaagt ttggtttgca gtatctattc
     3301 ctaatgtaat catatatttg atcctttctg ctatgacatt ctcgccttcc attgtagcat
     3361 acttttttat tctgaggatg aaaataccaa aggcaaaact agtacaataa tacatgatta
     3421 tctgctggcg ggcaaatttt gccccttcct gaaacacaaa aaagctgcgg tatcactcca
     3481 ttttcccaca gcttttccat cttttatttc gttgattctc gaataatcaa ctctgtttcc
     3541 acaataatct ttcttgaaac cggtgctttg gaattactta attgaagaat cgtctccact
     3601 gctagctctc ccatccattc ggtataaact tttacagtag aaagcgaggg cgttacatat
     3661 ttcgctacac tgacatcatt gaagcccatc acagaaatat cttctggtac cactaaacct
     3721 ttttcttgaa tcgctttcag tgcgccaatc gctaatgcat cattagctgc aaaaagcgca
     3781 tcaggaacat gcggattcgt ttttaaatac tgcttcactg cttgatatcc tgactcgaca
     3841 gaaaaatctg cttcgataat tggaccttcg tcgtagcctt tttctgccaa aacttgttga
     3901 aatgccataa ttctgggatc ttttagccgc tgatggtttt tcttcgtata ttctgttcca
     3961 gcaagcatag cgatatgttg cgctttatgg gagacaaagt gttcaatgac tttttgaaca
     4021 cttgacttaa agtcgacaac tagagagtcg attccttgag aagtgccgtc agaatctacc
     4081 aaaagcaagg cattttggga tcctttcatt tctaataact gagcttcgtc gaattttcct
     4141 agtgcgatcg ttccatctgc cggaagatca ctaatggaat cccacgtttc tttcaataaa
     4201 gtaacgtttg cttcttctgc ctttttttcg attcctaggc gaatagataa ataataaaga
     4261 tcttcaagct cttccgtatc gtcgtaccac tggatcaggc gaagcgtttg cttagtattt
     4321 gctgcatttt tacgatgttt cgtataattt aagcgttctg ctgcttcaaa aattttttgc
     4381 tttgtggcta agccaacgga aagatcagga tcatagttga gaactcttga aacagtagca
     4441 ggagaaactc ctgccaactg cgcaatgtct ttaattgttg ccatttgtct ctcctccttt
     4501 tttacgttta taaaatcgca gtaaatcgtt caaacgctgc ctgtcctttt tcatctcgct
     4561 tgaatactcc agcgtcttcc agtacacgtg caaaaacctg acctacagat ttttgtacga
     4621 catcggtgac cgtatctttc gtcaaatcag gatgttcttt ttttaattgt tctgcccacg
     4681 gacgatgata ttcagccatc tggttttctt ctcctaataa gaaatgctct acttctacta
     4741 gttctggttt caaacgaggt ggtaaaacag caagccccat tacttcgatc aacccaatgt
     4801 tctccttttt gatatgttgg acatctttat gtggatgaaa aatcccatca ggatgttctt
     4861 ctgaaacatt gttgtctcga aggacaaggt ccaactcaaa gtaattgtcc cttcttcgtg
     4921 caattggcgt aatcgtatgg tgtggtgtac catctgctga aaaagctctg atagatactg
     4981 tttcatccga atatgctttc catttttcca atactgagtt agctgcttca ataagcaagt
     5041 ttttctctgt tgattgcaaa cggatcacag acatcggcca tttcacgact cctgcgatga
     5101 cttgcggata atcttctagt ttgataagtt tttctatcgg tgcttttgcc ataggaaatt
     5161 catgacgtcc tgcctgataa tgatcatgag aaagaatcga tcctccaaca attggtaagt
     5221 cagcattcga accaacaaag taatgaggca agacttcagt aatccgaagc agtctagcaa
     5281 atgtttcctt tgtgattttc atcgggcggt gttgttctga taaaatgatc gaatgttcat
     5341 tgtaataagc ataaggggaa tattggaatc cccagctttc accgtctaga ttcattcgaa
     5401 taatccggtg attggtgcgg gctggatagt tcaagcgacc tctatatcct tcgttttcca
     5461 tgcaaagcat gcaggtagga tagtctactt ttaccgcctc gcgttctgct gcgatttgtt
     5521 ttggatcttt ttctggctta gataaattga ttgtgatttc cagttcacca tactccgact
     5581 ctgccggaaa tttgatgttt tttgcgatgg ctcttgtttt gatgtagtca tttttcttac
     5641 ttaaattaaa gaaatactca gttgcttcct ctgggctttt cgaatagtgc tgtgcaaaga
     5701 atgcattgac gactgaaggt ggcggtgtga gaaaatccat taattgcgct tcaaaaatct
     5761 cttgttctgc tggcaattct gaaagaacac cattttctct tgccttagcg acaagctgat
     5821 ccaataagtc tacagattct tccttaactg gtcttacttc aatgctttct agagcatctt
     5881 ctcctaccat ccctaaaaga cgattctgta aatataagcg gtccatttcc atccatccgc
     5941 cagcttcgat cgcaagtgtg gcaaaatcac aaatcgtttg actgattgac atatctcatt
     6001 cccctattct tcataaccat ttggatggct gacatgccaa tcccatgctg ttttgataat
     6061 cgctttgata tctgtgtatt caggtttcca gcccagaatc tctctcgctt tttcacttga
     6121 agcgactaat cggctaggat ctccagctct tcttggtgct atttctgctg gaatttcttt
     6181 tcctgtcact tctctagctg cttctagcat ttccttgact gagtatcctt tgctgctgcc
     6241 taaattgaag aagttgcttt catttccttc tttcaagtat tccaaagcta aaatatgtgc
     6301 agcaatcaaa tcttctactt gaacataatc tctgatacat gttccatctg gtgtatcata
     6361 atcgtctcca taaacagcca aggcttttct ttgaccaaga gctacttgta aaatgattgg
     6421 gacaagatgc gtttctggtg tatgatcttc tccgatagat gcatctgctt ttgctccggc
     6481 tacattgaag tagcgcaaag cgacatagcg catgccatat gcttggtcac accacttcat
     6541 cattttttcc atcatcaatt tactttcacc atacggattt ttaggattag ctggtgtatc
     6601 ttctgtgatt ggagactctt tcggctctcc ataagttgct gcagtagaag agaatacgat
     6661 tttattcacg ttgaactcgt gcatgacttc aagtaagatt tgcataccgt atacgttgtt
     6721 gttgaaatac atcaatggtt tttcgacaga ttctcctact aaagagcttg cagcaaaatg
     6781 gattactcct tcaatcggtt ccttttcaaa tacggaacgt agaaattctt tatcccggat
     6841 gtctccttca taaaaatgag catctggatg tactgcttgt ttgtgacctg tgagcaaatt
     6901 atctaccaca gctacttgat accccttttg aactagctgg tctacagcgt gcgaaccgat
     6961 ataaccggct ccgcctagta ctaaaattgt cataaaaaac actcctttga ttagtaaaat
     7021 tgtgtttgtt gattctatat aatttaaaaa acgaagttta cttttatggt gaaaacaaac
     7081 aaagtaaaat ttcgtaaatt ca
//
LOCUS       DAHHZJ010000141         7027 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.143, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000141 DAHHZJ010000000
VERSION     DAHHZJ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.143"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            225..944
                     /gene="atpB"
                     /locus_tag="NBL02_002296"
     CDS             225..944
                     /gene="atpB"
                     /locus_tag="NBL02_002296"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCD2572969.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            997..1212
                     /gene="atpE"
                     /locus_tag="NBL02_002297"
     CDS             997..1212
                     /gene="atpE"
                     /locus_tag="NBL02_002297"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HCD2572970.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            1303..1827
                     /gene="atpF"
                     /locus_tag="NBL02_002298"
     CDS             1303..1827
                     /gene="atpF"
                     /locus_tag="NBL02_002298"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCD2572971.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            1814..2356
                     /locus_tag="NBL02_002299"
     CDS             1814..2356
                     /locus_tag="NBL02_002299"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCD2572972.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            2379..3935
                     /locus_tag="NBL02_002300"
     CDS             2379..3935
                     /locus_tag="NBL02_002300"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCD2572973.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            3949..4851
                     /locus_tag="NBL02_002301"
     CDS             3949..4851
                     /locus_tag="NBL02_002301"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCD2572974.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            4874..6280
                     /gene="atpD"
                     /locus_tag="NBL02_002302"
     CDS             4874..6280
                     /gene="atpD"
                     /locus_tag="NBL02_002302"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCD2572975.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            6294..6716
                     /locus_tag="NBL02_002303"
     CDS             6294..6716
                     /locus_tag="NBL02_002303"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCD2572976.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         2278 a         1262 c         1524 g         1963 t
ORIGIN      
        1 aacatgatta tcataggttt tttcttagat caggagcacc gtgtataaag aaaaattcgc
       61 ttcgtttttg actgaaataa aaaagtgtct aattttcatg aaaatgtttt gaattttctg
      121 tttcatggtg gtatgatacc gtgtggttag aaaccgattg gaaaaaacca gatacgcaca
      181 tgaattgatt ttatgtgaat ttcggggaaa agaggtgaaa tggattggat gaacgctcgc
      241 taacgttcca cattggacca atttggtttg atggtacagt ttgtataatg gtgttactga
      301 cctgtttgat tgttttcttc ttagcttact tttttacaag aaatcttaag ttgaaaccaa
      361 caggcaaaca aaatgcgata gaatgggtca ttgattttag tcgaggaatc gtaacagata
      421 atctgcctag aaaagaattg agcaatttcc atttgttggc atttacattg tttttatttg
      481 tttttgttgc aaacaacatc ggtttagtaa caaaaatcgt tttacctaac gaaacaacac
      541 tttggaaaag cccgacagca gacccgtttg ttactttaac tttggcattt ataatgatct
      601 tacttactca cttatttggg gtgaaaaagt taggcttcaa aggatatttc gtaaattcgt
      661 tcttgaaacc atatagtttt atgtttccga tgaaactgat tgaagaattt acaaacttat
      721 taacacttgc tttacgtctt tatgggaata tttatgcggg ggaagttttg ctaaccttga
      781 ttgcaaaaat ggtaataagc ttaggctggt tctcattgcc gttagcaatt ccattagaga
      841 tggtctggat cgcattctcc ttgtttatcg gaagcatcca agcatttgtc ttcgttactt
      901 tatctatggt ttatatgagc cataagattg aagtggaaga ataatattcg ttttaaaaac
      961 aaactactat ttatctaatt taggaggaaa acaattatga attatatcgc agcagcaatc
     1021 gcaatcatgg gagcagcaat cggagccggt tatggtaacg gtcaagttat ctctaaaaca
     1081 atcgaatcaa tggctcgcca acctgaaatg tctggtcaat tccgtacaac aatgtttatc
     1141 ggggtagctc tagtcgaagc tgttcctatc ttaggggtag ttgtcgcctt gattctagtt
     1201 tttggtgttt aattgaatca gtattaaggc agaaacaatt tggtttgtta ctgctttttt
     1261 acatgaaaac tagaagtact tcaaagaaag gaaggctcgc ctatgctgaa tcaattggca
     1321 attgcagaag ttggcaatgc gatgttagga aatgtcatcg ttgtcagcgg ctcatttttg
     1381 atactactgg ctctcttgaa gcattttgct tggggaccaa tcagcgatat tttgaaaaaa
     1441 cgtgaagaca agatcgccaa tgatttggat tctgcagaac aatctcgcat caactcagcg
     1501 aaaatggaac aagaacgcga acaacaattg ttagcctctc gttctgatgc agctgatatc
     1561 atcaaaaatg cgaaagaaag tggagaatta agccgccaaa atattttgaa ggatgctcaa
     1621 gaagaagcag ctcgtctaaa aagtaaagcc caagctgata tcactgtaga acgtgattca
     1681 gcgctgaact ctgtaaaaga cgatgttgca gaactatctc ttcaaatcgc ggaaaaaatc
     1741 ttgaacaaag aattatctcc agaaatgcac gaatcattaa tcaatcaata tatcgaaggt
     1801 ctaggttctt caaatgaaac tagataaata tacagtagga agacgttacg ggaaagcttt
     1861 atttgaactg gcgatcgatt caaataaagc agaagaaatt tatcaagatc tcgtaagctt
     1921 acgtcaaatc tatgaacaag tacctggatt aggtaatatg ttaagtgatg ttcgtctaga
     1981 acctcatgaa aaacgagaaa tcatggacaa gcttgttagt ggctatgatg gaatcgttaa
     2041 aaacttttta gaagttgttt atgcgtataa ccgaatggat gatcttttgt tcatgatcga
     2101 agaatatgaa cgtcgatatg atgaacatca aggtttgctg ttgggaagtg tcacaacagc
     2161 agttccactt tcagacgaac aacgaataca attagaaaag aatgttgcaa aaacaatgaa
     2221 ctatcaatca gtcgaattaa aacaaatcgt tgattcttct attattggtg gcgcgattgt
     2281 tgaagctaac catcgagtaa tcgacggaag cattcgcacg caattagaaa atatgcgtaa
     2341 tcaattaaat agatagtaca aagaggtagg tgaatcgaat ggccatcaaa gcagaagaaa
     2401 ttagtgcctt gattaaagaa caaattgaaa attatcaaaa cgtgctttca gtcgaagaaa
     2461 tcggtaccgt aacttatgta ggtgacggga tcgctcgtgc acatggttta gaaaacgcga
     2521 tgagtggtga gttgcttgaa ttttcaaatg gctcatacgg aatggcgcaa aacttagaat
     2581 caaatgatgt aggtatcatt atccttggcg atttcgagtc cattcgtgaa ggagataaag
     2641 taaaacgaac aggaaagatc atggaagttc cagttggaga ggccttgatt ggacgggtag
     2701 ttaatccgct aggtcagcca attgacggac taggtgaaat cgttactgac aaagctcgtc
     2761 cagtagaagc aatggcacca ggcgttatgc aacgtaaatc tgttaacgaa ccaatgcaaa
     2821 caggtctaaa agcaatcgac gctcttgtac caatcggacg cggccaacgt gaattagtta
     2881 tcggtgaccg taaaacaggg aaaacttcca ttgcgatcga tacgatcatc aaccaaaaag
     2941 gtcaagatat gatctgtatc tatgtagcaa tcggacaaaa agattctaca gttcgtacac
     3001 aagttgaaac attgaaaaaa tatggcgcaa tggattacac aatcgttgtg aatgccggtg
     3061 cgtctcaacc agcgccgttg ctttatatcg caccatatgc tggtactgca atgggtgaag
     3121 agttcatgta caacgggaaa catgtcttga tcatttttga tgatctttca aaacaagctg
     3181 ttgcttatcg tgaactttcc ttgctgcttc gccgtccacc aggtcgtgaa gcttatccag
     3241 gggatgtttt ctacttacac tcacgcttat tggaacgtgc agcaaaatta agtgatgatt
     3301 taggtggcgg ttcaatgact gccttaccat ttgttgaaac acaagctgga gatatctctg
     3361 cttatatccc aacaaacgtt atttcgatca ctgatggaca aatcttctta gaaagcgact
     3421 tgttctacgc aggtactcgt ccagcggttg atgccggact atctgtatct cgtgtagggg
     3481 gttctgcgca aatcaaagca atgaaaaaag ttgctgggac tttacgtcta gacttagcaa
     3541 gttaccgtga attagaagca tttacacaat tcggttctga cttggatgca gcaacacaag
     3601 caaaactaaa tcgtggacgc cgtacagtag aaatcttgaa acaaaaattg catgcaccgc
     3661 tagctgttga aaaacaagta gtgattttgt atgctttgac acatggattc ttagatagta
     3721 tccctgtaga cagtatttta gacttcgaac atgaattatt cgaatatctg gataccaatc
     3781 attcagatat ctttgaaaca atccgtacaa caaaagatct tccagaagaa gaagcgttga
     3841 acagtgcgat tcaggaatat aaagacatgt tcttagctac aaaaggaaat aattcctcta
     3901 cagaagataa attgaaatcc attcaaaatg catagtgagg tgagatgaat gggtgcttca
     3961 ttaaatgaaa tcaagacacg tatcgcttca acgaaaaaaa cgagccaaat cacgcgggca
     4021 atgcagatgg tgtctgcttc taagttgaca aaatctgaag cttcttcaca gaagtttcag
     4081 gtttatgcaa caaaagtaag agaaatcgtt acgcatttga ctgctactca gttaaatgat
     4141 attgcctcag aaaatcctag aggggatatc aattacaaca gcatgttgct cagccgtcct
     4201 gttaagaaaa cgggttatat cgtcatcact gcagatggcg gtttagtcgg tggatataac
     4261 agctcgatat tgaaacaaac gatgtctatt ttagaagaag accaccaatc acccgatgat
     4321 tacgtgatga tcgcaattgg cggaactggt gcagacttct ttaaagcacg cggtatcaat
     4381 ctagcatatg agctgcgcaa tctatctgat cagccaagct ttgatgaagt ccgcaaaatc
     4441 gtaaatatgg caacaactat gtaccaaaac gaagtattcg atgaattata cgtatgttat
     4501 aaccatcata tcaattcgct aactagtcag tttcgtgtag aaaaaatgct gccaatctca
     4561 gacttagatc cagaagaagc aactactttc gaacaagaat atatttttga accttcaaaa
     4621 gaagaaatct tgtctcagtt attgcctcag tatgctgaaa gtttgatcta cggtgcgatc
     4681 gttgacgcca aaacagcaga acatgctgct ggtatgacag caatgaaaac agcaactgat
     4741 aatgctgcta atatcattga tgagctgacg gtttcttaca accgtgcaag acaaggggct
     4801 attacccaag aaattaccga aattgttgcc ggagcttcgg cactagaata gtattgagat
     4861 tggaggaaaa aagatgagtt caggcaagat tgttcaagta atcggtcccg ttgtcgacgt
     4921 ggaattttct ttagaccaat ccttaccaga tatcaacaac gcattagttg tttataaaaa
     4981 tgacgaaaat aaatcaaaag ttgttcttga agcagcgtta gaattagggg acggagtgat
     5041 ccggaccatt gcgatggaat caacagatgg tttacaaaga ggaatggaag tcattgatac
     5101 aggcaaagca atctctgttc ctgtaggtaa agaaacatta ggtcgcgtat tcaacgtact
     5161 aggagatacg atcgatttag aaacaccttt cccagaagat gcggaaagaa gcgaaattca
     5221 taaaaaagca ccagcctttg acgaattaag tacaagtaca gaaattttgg aaacagggat
     5281 caaagttatc gatttgcttg ccccatattt aaaaggtggg aaagtcggac tattcggtgg
     5341 tgccggtgtt ggtaaaaccg tactgatcca agaactgatc cataatatcg cccaagaaca
     5401 tggtggtatt tctgtattta ccggtgtagg tgaacgtact cgtgaaggta atgacttgta
     5461 ttatgaaatg aaagattcag gagttatcga aaaaacagcc atggtgttcg gacaaatgaa
     5521 cgaaccacca ggtgcacgta tgcgtgttgc tttgactggt ttgacgattg cggaatattt
     5581 ccgtgatgta gaaggtcaag atgtactgtt gtttatcgac aacattttcc gtttcactca
     5641 agctggatct gaagtatcag ccttgttagg acggatgcct tctgcggttg gttatcaacc
     5701 aacattggca acagaaatgg gtcaattgca agaacgtatc acatctacga aaaaaggttc
     5761 gatcacatcg atccaagcaa tctatgtgcc agccgatgac tacactgacc cggcgccggc
     5821 aacagcattc gcccatttgg atgcgacaac caacttggaa cgtaaattaa cagaacaagg
     5881 gatttatcca gcggtagatc cattggcttc ttcttcaagt gctttagcac ctgaaatcgt
     5941 gggagaagaa cattataaag tagcaacaga agtccaacac gtattgcaac gatatcgtga
     6001 attacaagat atcattgcta tcttaggtat ggatgaatta tccgatcaag aaaaagtgtt
     6061 agtttctcgt gcccgcagag tccaattctt cttgtcacaa aactttaacg tggctgaaca
     6121 atttactggt cttccaggtt catatgtccc tgtagaagaa acagtgaaag gattccgcga
     6181 aatcttggaa ggcaaatacg atgatcttcc tgaagaagct ttccgaagtg ttggccgaat
     6241 cgaagacgtc gtagaaaaag cgaaaacact aggctactag aaaggggtgc cccatggatc
     6301 aatatttaac agtaaatgtg gtaactcctg ccggtttggt ctatgatcac catgcagcta
     6361 tcgtcgttgc acgtacgaca gctggtgagc ttgggatttt gcctaaacat gcaccaatca
     6421 tcgttccatt gacgattgat gaagttcgtg taaaacgaac agattcagat acccacgtcg
     6481 attggatcgc tgttaatggc ggaatcatgg aagttcgtga taatgttgtt tcaatcgttg
     6541 ctgatagtgc ggaacgtgaa cgtgatatcg atgtatctcg tgcggaacgt gcaaaacaac
     6601 gcgcagaacg ccaaatcgaa gaagcaaaag caaaacaaga tacgaacgaa ttgaaacggg
     6661 caactgttgc gttgcaccga gcaatcaatc gtatcaaagt atctaagcac tcataaaatg
     6721 agatgcggat gtttatccgt gtttaagaac agtaaaaaga ggttatgaca gaagtggtca
     6781 gcttcaagac ataagaagga atttacgaaa attgcttctc aaatttttgt gaaatctcag
     6841 cttatttttc gaagaagcta cttctggaac accgtatatc ggaggttgtg aaatcagcgt
     6901 tttgtcctga gtattagaga aacaaatgga aaatagttcc tcatattttg ccgttttttg
     6961 agctaatacc gagggtcaga tgattgaaca ccgtttattt ggaataaggg actgcgacaa
     7021 gactttt
//
LOCUS       DAHHZJ010000142         6902 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.144, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000142 DAHHZJ010000000
VERSION     DAHHZJ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.144"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            253..528
                     /locus_tag="NBL02_002304"
     CDS             253..528
                     /locus_tag="NBL02_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572977.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            541..2619
                     /locus_tag="NBL02_002305"
     CDS             541..2619
                     /locus_tag="NBL02_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HCD2572978.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2779..4665
                     /locus_tag="NBL02_002306"
     CDS             2779..4665
                     /locus_tag="NBL02_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCD2572979.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4677..5624
                     /locus_tag="NBL02_002307"
     CDS             4677..5624
                     /locus_tag="NBL02_002307"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCD2572980.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5644..6171
                     /locus_tag="NBL02_002308"
     CDS             5644..6171
                     /locus_tag="NBL02_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCD2572981.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6234..6887)
                     /locus_tag="NBL02_002309"
     CDS             complement(6234..6887)
                     /locus_tag="NBL02_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCD2572982.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
BASE COUNT         2323 a         1165 c         1466 g         1948 t
ORIGIN      
        1 tgtcgcagcc ccattttttt gttgtgataa aagaacaaga aaggcaggaa tgggaaagta
       61 aacatccttt tgtcagaaac ttttatcttt cttaagtttt acttgatatc tgatactatt
      121 tggaatagaa tgaggcgttg aaaaatttat ggaaatttct aactagcaat aaacgatgaa
      181 acaatgaaat atttttcatc caaaaagaac tttgctataa tgatggaagt ttgtgtatag
      241 gagatctgga aaatgagaga gagttttgaa caacaaaaaa aactgttgca tgatcgctat
      301 ggcgcccttt cgatggatga ccgccgtcaa atactttgta agttgcgtaa aagaaatata
      361 ttgatgtatc gtcagttgga acgtctgaaa catgatctgc ttcgtttgga gtcaaaacgt
      421 gtacagtgtg aattagaggg gaaccaaaca caggttgaag tagtcgaaac caaaatttta
      481 aagaaaaaag aacaattttt aaaaatgctt acacaaaata aaaaataaag gggatggacg
      541 atggaatttg ctgagttagt tattgtattt gcagtaacga tcaccttatc gaacatagct
      601 agccgcattt tgccaatgat tccagctcca ctgatccaaa ttttccttgg agtggtatta
      661 ggtctgacag agtggggaca atcaatcgat tttgaacctg aattgttctt agttatgatt
      721 attgcaccat tgttatttag agaaggagaa aaagcagata tttcttcgat tctcaaaaat
      781 ttcgggacaa ttttgttctt ggcatttgga ggagtcattt tgacccttgt cggggtaggt
      841 gcgacgttat cattcctgtt gccaagtgtc ccacttgctg cctgctttgc ttttggagca
      901 gcgttaggtc cgacagatgc ggtagcagtc agttctcttt ctggacgagt gaatatccct
      961 aaaaaggcga tgcatatttt ggaaggagaa ggtcttttga atgatgcatc tggtgttact
     1021 gccttccagt ttgctcttgg tgcgctgatc accggatctt tttcagcagt aaacgcgggg
     1081 atgagtctag tagtttctag tattggggga gctttgatcg gctttttgct tgtctggttc
     1141 aaacaaaaaa taattcattt gatcgaaaaa gcatcagcac aagatgtcac agggtatctg
     1201 ctgatcgaac ttttgcttcc ttttctagcc tatgtattgg cagaggcatt tgatgtatct
     1261 ggaatcattg ctgcagtggc agcaggtatt ctccaagctt ctggttttag gaaaatctct
     1321 gtgtttgatg cagaattgtc gagcttgtcc cacagtacat ggacaactat tgcttttaca
     1381 ctgaatgcat tagtatttat atttttagga atcgaattga cgcaagtgtt ctcgccagta
     1441 tggggagacg gtttatatcc aaacggtctt ttattagcga ttatcgtatt aatctctgtc
     1501 atgctttttg tgatcagatt catttctatc agtctttttt atgtattcaa agatggcagt
     1561 aaaaagttca aaaaacagtt gaacgagatc ttgatcttaa catttggcgg tgtaaaggga
     1621 acagtcagtt tggctacgat ttttattttg ccattttcaa tcaataatat gatgttttac
     1681 cagcggtctt tgcttttgtt tctgacggcg ggtgtcatct tagtgacttt ggtcataggg
     1741 atcattgtct tgcctatgct gacggaaaca gaggaagcga aaagcacgga tttgaatgct
     1801 ttgatgattt tagaagaggt cgttgaagtt ttgcgaaaag aaataaagga aattgatcgt
     1861 aatacaaaag aatttttagc cactgaagca gtgatagaaa attatcaaga acggatacgt
     1921 gatctgtact tggaagattt aaccgatgat gaaaagcaag aagtacaaga aatacaagca
     1981 ctgattttgt cgatcgaacg ggatggattg gatgaaagtt atcgttcggg caagctttct
     2041 tctaatggtt atcgttttta ctcccgtttt ctttctcgat tcgaacattc tatcacaagc
     2101 cagattttgt cttttatcgg cttttggttc attgttgtcc gtaggttaat gcgaatcgtt
     2161 ctccatccga aaatgttttg gcaaagaaga tatgctgatc gtcagacatt tattagtgat
     2221 gaagatatcc aagaaatcag agagacatat caaaaaaata ctcagttgat tattgaaagt
     2281 ttagataact tgactgatat ttatgatgaa acgatggttc aattttttat ccagcaacgc
     2341 aaacttgaag gaataaaaat gaactcgggg aatttgattt cttcgtggat gatccagcaa
     2401 gatagtctgt ttacgaaaaa aatgctaaga ggttattatc ttgaaagaaa aatcattgat
     2461 gaatacgaag ttgctgaaaa aatcacgacg ttttctgcga acagctatcg caggaatatc
     2521 aatttgttag aatcttatac gatgaacaaa ccatctgata acttttcttt ccgatttgcg
     2581 tttcgagcaa agtcaaaaaa aacgagtcca ataaaataaa aaggtaaaaa ttttcaagtt
     2641 ttataaaaaa caaaacatat cagaacagga tcgaatcggt cctgttcttt ttttgagaac
     2701 acaaaaagaa caaataccaa aaaaattttc ctgaagttca ctatttttat gatacaatat
     2761 ccaagataag gagggaacat gttgaaagaa ttaaaagtaa ctgacctcaa aaaaacttat
     2821 ggtgaaaaag atttgtttga ccagatttct tttttggtcc acgaaaaaga ccgtatcggg
     2881 ttgattggga cgaacgggac gggtaaaaca agtctcttaa atattattgc cggtatcgat
     2941 agcagcgacg gtgatcggca aacagtgttt tatccaactg attaccgtat tggttatctt
     3001 tctcaagcag ctgaattttc cgatgaatta actgttctcc aagcagtctt tcaaggaaac
     3061 agccccttga tccaaactgt tcgagcatat gaggaagcat tgatcgaact aggggaaaac
     3121 ggagaagacc ctgatgtgca aaaacgttat gctaaagcag aagaacaaat gaataaagaa
     3181 gatgcttgga ctacagatac caacgcaaag attattttgc aaaaattagg aatcagtgaa
     3241 ttagataaaa aaatcagtac tttatcaggt ggtcagaaaa aacgagttag tctggcgcaa
     3301 gtgttgattg atgaacccga tttactatta ctagacgaac caacaaacca tttagattat
     3361 gaagcgatcg agtggttgga aaactatttg aaacaatacc gtggcgcact attgatggtt
     3421 acccacgatc gttattttct agatagagta gcaaaccgaa tcttcgaact ttcatttgga
     3481 aaattatacg agtataaagg aaattatgaa gcttacgtct tagaaaaagc agaacgtgat
     3541 cgtgtagaag tagaacaaga agaaaagcgc aagcggcttt ataaacagga attagcttgg
     3601 atgagggctg gcgtacaggc acgtggcacc aagcagcaag cacggatcaa ccgtttcgaa
     3661 gatctaaagg agaacttgta ccaagtaaac caagaagatg atttagagct gaacctagcg
     3721 acacaacgtt tagggaaaaa agtactggaa atcaaagatg gcagttaccg tataaatgac
     3781 caaacactgc tagaacattt agatttattg attcaatctc gggaaagact aggtattact
     3841 ggtaaaaatg gtgcgggaaa atcgacattg cttaatattc tagctggcag aattcccctg
     3901 gatagcggta caatgtctat cggtgaaacg gttcatcttg cgtattatac acaagaaaat
     3961 gaagaaatgg caccagataa acggatgatt gcttacttac aagaagctgc tgaagaagca
     4021 aaaacagcag atggcagtca aatcggagta gcagaattac ttgaacgatt tttatttcca
     4081 agatttatgc atgggacact gataggcaaa ctttcaggcg gagaaaaacg ccggttattt
     4141 ttgttgaaac tgctgattca gcaacctaat gttttattat tggatgaacc gaccaatgat
     4201 ttggacatag caacattgac gattttggaa gattatttta gaagttttcc aggtgctgtg
     4261 atcactgttt cccatgaccg ttatttcttg gacaaggtag cagataagtt attagtattc
     4321 caaggaaacg gaaaacaaga actttattac ggaaacatgt ccagttattt actgaagcaa
     4381 aaagagaccc agcagcctgc tgaaaaagcg aaaccaaaaa tacagagcaa ggaacctgct
     4441 gaaaagaaga aattatctta catggaacaa aaagaatggg aaacgattga agatgagatt
     4501 gcagaacttg aagaaaaaat ctcattgctc caagaagaga tgaatcatca aggagataat
     4561 ttcactcgat tacaagaatt gcaaaacgac gtatctgaaa cagaagcaca acttgaagaa
     4621 aaaatggcac ggtgggaata cttgagtgaa tgggtagaag attaggagga aaaaggatgg
     4681 aacaagcgta tcttgactta ggaagaaaat tgttggaaga agggcatcgt aaagaagaca
     4741 gaacaggcac aggcacatta agcttattcg gataccaaat gcggtttgat ctgcaaaaag
     4801 ggtttccttt gttaacaacg aaacgagtac cttttggatt gatcaaaagc gaattgcttt
     4861 ggtttttaaa aggagatacg aatatccgct atctattaca aaataaaaac catatttggg
     4921 atgaatgggc atttgaacgc tacatcaaaa gtccagacta taatggacca gatatgactg
     4981 attttggcca tagatgtttg acagatcctg aattcaatga agaatataaa aagcagagca
     5041 agcttttttg cgagaagatc ctaaccgatg attcctttgc tgaaacatac ggcgatcttg
     5101 gacacatcta cggctatcaa tggcgtcact gggaaacaaa agacggtggc tttatcgatc
     5161 aaatcaaaca agtgatcgaa gcaatcaaaa agactcctga ttctagaaga ttgatcgttt
     5221 ctgcatggaa tccggaagat gttcctagca tggctttgcc tccttgtcat acgatgtttc
     5281 aattttatgt tcaagaggga cggctaagct gccaattgta tcaacgcagt ggggatgtgt
     5341 ttctaggcgt tccgttcaac attgcaagtt atgcgttgct gactcaccta attgcacatg
     5401 aaacaggttt agcggttggc gaatttgttc acacattagg agatgctcat ctttatatca
     5461 atcatatcga acaaatgaaa gaacaattga gccgagaaat acgcgcattt ccgactttag
     5521 ttttgaatca ggaaaagcag tcagtatttg attttgagat ggaagatatc caattagaag
     5581 gatatgaccc acatccggcg atcaaagctc ctatcgctgt ttagaatcag aaaggatgag
     5641 aaaatgttag ctgcaatttg ggcacaggat gaaaacggat tgattggaaa agaagaccgt
     5701 cttccttggc gtttgccaaa tgatctgaaa ttcttcaaac aaatgacaga agcaaataca
     5761 ctagtgatgg gacgaaaaac attcgaaggg atgggtagcc gtccgttgcc gaatcgtcag
     5821 acgattgttt tgacgagaga ttcttcctat aaagcagaag gcgtgcatgt catgcatagt
     5881 gtggaagacg tgcttgctct ggaaaaagaa acagacggta tctttttcat cgctggaggg
     5941 tcagctgttt atcaagaatt tctgccattt tgtacgatat tataccgtac agtcatccat
     6001 catgtattcg atggagatgc ttattttcca cctgttgact ggtctgattg gtcactgatc
     6061 aatactagtc aaggagaaat ggatgaaaaa aatcgttatc cgcatcaatt tgaaacgtat
     6121 cagcgtaatg agaatcctgt aaaaagcggg acaggcgaat tgacgatata aaaaagctat
     6181 ggatagacta gctgttattt agagctgtcc catccatagc tttatttttt ttattaagta
     6241 aagaataaaa cagagaagta cataaaacct gcacctaaca taacaaacaa atgccaaaca
     6301 acatgcatat agcggataga attcaagcta taaaagaatg cgcctatcgt gtaagaaagc
     6361 ccgcctgcta ccatcaaaat aacaccagtc attccaagag agtgataaag aggaacagca
     6421 gccacgacac agagccaccc cataaaaata tagatgatcg tagatacttt tgatactctc
     6481 tcccgtttat gcaacgtcaa cgctttatat ataatgccaa gaatcgcaaa caaccagatt
     6541 aatacaaata aagtccaacc tagccatcct cggatactca gcaaacaaaa aggagtataa
     6601 cttcctgcaa tcaaaagaaa aatcgaacta tgatcgaata cttggaacac cttttttgct
     6661 tttgtaaaaa tcaatgaatg aaacaaagta gacgttaaaa ataaaagaat catcatcgat
     6721 ccatagatag cataagagac aatatggagg gaggaatgca agtgggcacc ttttactaaa
     6781 agaaccacta gtcccgcgat actcaatcca gtcccaattc catgggtgac tgcattaaac
     6841 acttcattta atatttcata cgttcgatta aatttcgttt tttccatgaa aacggctcct
     6901 tt
//
LOCUS       DAHHZJ010000143         6885 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.145, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000143 DAHHZJ010000000
VERSION     DAHHZJ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.145"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(<1..563)
                     /locus_tag="NBL02_002310"
     CDS             complement(<1..563)
                     /locus_tag="NBL02_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCD2572983.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(750..1400)
                     /locus_tag="NBL02_002311"
     CDS             complement(750..1400)
                     /locus_tag="NBL02_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2572984.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="NBL02_002312"
     CDS             complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="NBL02_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HCD2572985.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2912..3910)
                     /locus_tag="NBL02_002313"
     CDS             complement(2912..3910)
                     /locus_tag="NBL02_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HCD2572986.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="NBL02_002314"
     CDS             complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="NBL02_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCD2572987.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4926..5657)
                     /locus_tag="NBL02_002315"
     CDS             complement(4926..5657)
                     /locus_tag="NBL02_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HCD2572988.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5661..>6885)
                     /locus_tag="NBL02_002316"
     CDS             complement(5661..>6885)
                     /locus_tag="NBL02_002316"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HCD2572989.1"
                     /translation="LGSDMNVYGMARSFNEAYGKICQAYASDQLAPTRYSKIVNVEVI
                     PGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQHKQELSEYFICPYID
                     YSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQELPFEFPVALKPANSV
                     EYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFIPGDDSNMRVLNAYV
                     DEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKAFLEKIEYTGFANFD
                     MKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFNKPFVETTYGTNQSD
                     KARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKDRSIKRWLLMKYMFH
                     NYIPRFKKYFHVKEG"
BASE COUNT         2065 a         1468 c         1074 g         2278 t
ORIGIN      
        1 tctataaaca tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac
       61 aaccttgatc cttcttttat agattcagga aacagtataa ttttagtact agataaattg
      121 tttacaccga ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac
      181 aaaagaacag ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta
      241 taaatactct ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct
      301 atccaagcaa cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt
      361 aatctattta tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa
      421 ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa
      481 taacctgatt ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat
      541 atccataatc tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag
      601 catcaccaat tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc
      661 atgcttcaac aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac
      721 ataaatttta cattctgttt tttataagac taataataat cattgaaaaa atttcttatc
      781 ggtggcgcat attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct
      841 tcttctaagt catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc
      901 tgtggcttgc ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt
      961 gttacatctt cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta
     1021 ttatttaaaa gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa
     1081 cttcctataa attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca
     1141 tattcaagac taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt
     1201 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
     1261 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
     1321 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt
     1381 tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt
     1441 caggtataac gtataccaac catacatccc aattactatt tatgaactta tgattatcac
     1501 tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc
     1561 gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac
     1621 cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa
     1681 tctccagtta tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa
     1741 gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
     1801 taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga attatagttt
     1861 gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc
     1921 atgtatcagt tttttctttg gattaaactg cttcttcacg cagtttttcc gcaaattcat
     1981 ttattttgcg aattcggtga ttaattcccg attttgatat tgcacctgag ggaatcattt
     2041 cccctaattc tttcaagctg acctctggat gttgaagccg aagttctgcg atttcctgaa
     2101 gtttttcagg aagggcatgt agaccgacct ttgcttcgat caattcgatg ttttcgattt
     2161 gtttagaagc cgcatcaatc gtcttgttca gatttgctgt ctcacaattg actagacgat
     2221 tgaccgaatt tctcatatcc cgtacgattc gaacatcttc aaatcggagc attgagttgg
     2281 ttgcaccaat caatgttaag aaatcagcaa ttttttctgc tccttttaaa tacgaaatat
     2341 agccattgcg ccgttcaagc gttcgagcat gtagaccgta ataatttaac atatcgcaaa
     2401 tgtcttggtt atgctgttca taaatagaaa atatttccaa atgataacga ctcgtttccg
     2461 gattattgac tgaacctgag gccataaaag caccacgtaa gtaagaacgc attttttgcg
     2521 aatttcccat aatctcgtcc gaaacgtgtc cattgaacat cataccatcc atgatatcaa
     2581 gatccatcaa cacatctttt gtgccttgct tcaaccggac gatatagaca ttgttctttt
     2641 ttaatttcat ttttttacga acgagtagct cacttcgtac ctgataatga tctttcaaaa
     2701 gagaatagat acgtctggca attgcagcat tttctgtttg gacgttcaaa ataaactgct
     2761 ggttcacaat acctaaagag ccgttcattc gcagcaaagc agctaattcc gctttcgcat
     2821 gttctcgatg aacttccaaa ccagttagtt ctttcttcac atctgcagca aatgacatcc
     2881 tattctcctc ctttctccaa caataaattg cttaatattt tgaaccgaaa actaaacgga
     2941 acaattcttc tactactttg tctccatcgt ggaagacacc accatctttc aattctaaaa
     3001 agtcggtgga aatcactcga catccttcat ttctcaatcc gttgaagtcg tgtgcgactt
     3061 gtactagata ctcatcatag atctctgggt ccatataatc ttcaggcact ttttccgtat
     3121 tgaccaatac tgtatcgata aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat
     3181 cggcatctgt gaaatgttcc gtttcccctt tttgggtcat gatattacag atatagacgg
     3241 tttcagcagt tgtcttgagc attgcttcgc cgatttctgg tatcactaaa tttggaagga
     3301 tactggtgaa taaacttccg ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa
     3361 cgacttttcg agccgctttg gcttcacttt tatcatttgt gttgcagaca tacacacggt
     3421 caatggtttt acggttgatc gcgattttag attctcctac tgcagtcgtt ccgtctttaa
     3481 aaacagcatg caaggtcaaa ggagtttctg aagaaggata aacatgtcca tctacatgca
     3541 tcatttttgc taataattga atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg
     3601 caatgatcag attaccgatc gtatgattgg ctaaaaactg atcttccttt ttgaaacggt
     3661 attggaaaat atcttcatag aattgtggca tatccgataa cgccactaag acatttctca
     3721 agtcaccagg aggagccata gcgatcgatt ctctgattgc accgctgctt ccaccatcat
     3781 ctgcaacagt cactactgct gtgatatctg ctccttgatt tcgtaaactt ttcaggataa
     3841 ctggcagacc agtgccaccg ccgaccacta cgatctttgg ttttcttatc cggtaggttt
     3901 tcattttcac gaccggttca ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt
     3961 gactttatag tttttcccta aagcatttgc caaacgttca gtcaatgcca cagaacggtg
     4021 ttgaccgcct gtacagccga ttgcaactgt caagctgctt tttccttctt taatgtatcc
     4081 aggcaataca gtttccagaa gatccaaaaa tttatggtag aatgattgcg tttcatcaaa
     4141 ggacatcaca taatcataga ctggctgatc ttttccggtc aatggtctta gttccgggat
     4201 ataatgaggg ttgggcaaaa aacgcacatc catcacaata tccgaatcga tcggcaaccc
     4261 gtatttaaaa ccaaatgata caaattcgat ccgaaatcct tggtctccct ttgatttgaa
     4321 tgactcattg attttttcac gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac
     4381 ggaagcttgg gctttcaagt cgtctagtat cgcccgttct ttacgtatcc cttcagtgat
     4441 caaaccgtcc atcgccattg gatgtgctcg tcttgtttct ttgtagcgag ccactagttc
     4501 ttcgtctgaa gcatctaaaa ataagataga agtgtcgatg aagttcgtat tttcgatttc
     4561 gatcagcatg ttttggattt cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc
     4621 catttttgtg actttacctg attctttgat caattcccag aattttggaa tcaatcgagg
     4681 cggcatatta tcgatacaaa aatagcccat atcttcaaaa ctttggatag ctactgtttt
     4741 ccctgcacca ctcatacctg ttatgatgac taattgcaaa ttatctgtca tatttacgaa
     4801 cccctctcac attgtgcata ataaaattat atcatcccgg gcgtatcatt accagttttc
     4861 tttttctttt acaaaaagat ttggaccaaa tatcttttag gatatttgcc ccaaatccaa
     4921 tttttttact tttccgatgc tgtatccaat gcctcgcttc tttcagctaa tgcacgttcg
     4981 atcgttcgat ccgctaaaat agattgagca tcgatcactg ggtaatgatt atcttcagca
     5041 aattcatgca tcaaagataa ttctgtgcaa cctaggatga ctttttcaca gtttaggcgc
     5101 tctaccgctt cctctaagat ttcgtaatag agctcttgat tcaaataatc cgattccttg
     5161 atctcatgat agatcaggta attgattttt tcttgaagcg cagtatcagg aatcactgtt
     5221 tcgaaaccaa gatttttgac ttcgcgttca tagattccag ctttcatact tccttctgtt
     5281 cctagaatcg ctactctccc tgtcgtatgc tgacggacaa gttcatttgc tgcttctctt
     5341 ggcatatgca agataggaat atccgttgcc gcttgtaact cttcaaaaaa gtagtgtgcg
     5401 gtgttacaag tcaacacaat gaagtttggt cgcaataaat tttgtttctc aatatcatcc
     5461 agcagaaacg gcattggatt ttcttctgat cgatctaaaa tataagctgt acgatcagga
     5521 actgttgcat gattgaataa gacataattc aaatattctt gatcttttgt agctttcgtt
     5581 cggtggttga tcaagcgaac aaaactttct gtcgccatcg tgcccattcc gcctaaaata
     5641 ctgaaaaaat tctccatgaa ctagccttct ttcacatgaa aatacttttt gaatcttgga
     5701 atatagttat ggaacatata tttcatcaac aaccagcgct tgattgatcg gtctttttca
     5761 taaaatacag tagtaccgta acgattttct ttgatcattt gctcagctag ttttttgtct
     5821 tcattttccc gagcatattc taaaaagatt tttttgggca cacccatcca taatcttgct
     5881 ttatctgatt gattcgttcc ataggtcgtt tcaacaaagg gtttgttgaa cactcgatct
     5941 tctgtgacaa aacgagccag attcaagccg ttcaatgtca caaagaaact gctacgacct
     6001 tgtctcaaat tgatttcaaa taatttatat tctccatctc taggatcata cttcatatcg
     6061 aaattggcaa agcctgtata ttcgattttt tccaaaaatg ccttaatggt ttgatagatt
     6121 ttttcgttat aatctggcat gatcacaacg taattaccaa tcgatgctgg tgtaggatct
     6181 tctaatagtg gatgaccaag gcacatcatc cgaacttgat gatcttcatc tacatacgca
     6241 tttagcacac gcatattact atcatcaccc gggataaaat cttgtacaat catttcactt
     6301 ttataaccgg cttcgtatat ccggcctaaa attaaatcga actcttctct agtctctaaa
     6361 ataaaagctt tttttcttcc ttcaaattgg acagaaagat actccacact gtttgcaggt
     6421 ttcaaagcaa ctgggaattc aaaaggcaat tcctgttcca aatgtccatt tactaacatt
     6481 tcttctctga cgatcaatgt tttaggatac ggaagatcat attcttcaca aacttcataa
     6541 aagcttactt tattgatcag cctttcaaat aaagaataat caatatatgg acagataaaa
     6601 tattctgaca gctcttgttt atgttgggag atcaattctg catatccatc accacaagca
     6661 atcaaaagat attttttact cttatcagaa tatctttctt ttgccaaacg aagcatcgtc
     6721 tcgataaata cagggtcttt atcaaatcct ggaataactt ctacattgac aattttactg
     6781 tagcgggtag gggctaactg atccgaagca tatgcttggc agattttgcc gtatgcttca
     6841 ttaaaagaac gtgccatacc ataaacgttc atgtcgcttc ctaat
//
LOCUS       DAHHZJ010000144         6813 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.146, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000144 DAHHZJ010000000
VERSION     DAHHZJ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.146"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(<1..2849)
                     /locus_tag="NBL02_002317"
     CDS             complement(<1..2849)
                     /locus_tag="NBL02_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HCD2572990.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEE
                     QIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLD"
     gene            complement(2863..4080)
                     /locus_tag="NBL02_002318"
     CDS             complement(2863..4080)
                     /locus_tag="NBL02_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCD2572991.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(4070..5665)
                     /locus_tag="NBL02_002319"
     CDS             complement(4070..5665)
                     /locus_tag="NBL02_002319"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HCD2572992.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            5790..6575
                     /locus_tag="NBL02_002320"
     CDS             5790..6575
                     /locus_tag="NBL02_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HCD2572993.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
BASE COUNT         1986 a         1388 c         1133 g         2306 t
ORIGIN      
        1 ctatctaaag tccccacatc aaatgcattt aaaatttgtc ggtaaacttc tttgactcct
       61 tctgttttat tcaatttagc caaagctttt tcaatttctt ctcgtgcatc tgaactattc
      121 gcagcataag ttcctagtaa ttgatcaata taagtagaat caatatcata caacttagtg
      181 gtattttcac tataaaattc gatcgttgaa aaatcttctc cctctttttc tggttcttcc
      241 tccatcaatg aacccttcac ggtctcgtac acgccaattt gttcttccaa aagttgaatt
      301 tgattgttca aagttgaaga ttgcaccatg tcatcattgt aatcatcata agtcactaac
      361 gattcatacg cacgattcaa ttcttgaaat gctttgacgt actcgatacg gatttcaagt
      421 ggcgtatgtt catcaatgga ttccatggat gaaacctctt ttaacttacc atatgcttgc
      481 ttgaatcttt ttttggattc ttggtaactt ggataaatca aacccgattc ttctttagct
      541 tctgaataca gttttgtcgc atcctcgaca ttcttctcca ttgtcgctgg ctttcggaaa
      601 gtaacaatta gtccttttgt tttttcagga tacgtacggt tcgtacgtga aaaggcttgg
      661 atcaatcctg catactctag attacgatcc acaaataggg tttgaatcgt aggggcgtca
      721 aaacctgtca atagacgatc cactacgatt actaaatcga tttgtttccc aaattgtttg
      781 aattccgctc tttttcgtgc caaacgatta ttgatgtctc cgttataacg ctcaatgtct
      841 gctagtgacc aggcagtatc ataatactga ttatattcct caataatttt ttgcatttct
      901 tcttgttggg cacttgaatc tcggctattt tcttctaatg aataggtaat agcgattcgt
      961 gggaaatcag gatcctccat cgttcgtcct tcacggatag gctgattggg aaactctttt
     1021 attaaccaat cagattcttt cgtcatctct ttgatggccc gataatactt tttggccata
     1081 tcaatggaac tcgtggttaa gatcgcagac ttcgttggac ggccatttcg aaaatcaaat
     1141 tttgtatagg cattgtctgg acggaacatt ttatgaatga ctttttggat atgttcctct
     1201 gcttcaaaaa cagtagggtc caaataactt tcttttttga cagactccat ttgatcgatc
     1261 atacgattga tctgttccgg tgaatactct gcatatgttt ctacttcttt taatttccga
     1321 tagattttat tctctaatga agtcggctcc acggtgtttt catgctctac ttgaaatcct
     1381 aaaaccgagc catcttctaa tgcattcttg atggtatagg tgtgcaagac ctctccatac
     1441 tgatcatggg tcgtacgtgc caattgccct ttggcttgtt tccgattctc agggaaaatc
     1501 ggtgtacctg taaagccaaa ccaagtggat ttcggaaaca tttttttgat ttcttccata
     1561 ttttcagcac ttaacgctcg atgacactca tcaactacaa acacgatatg ttgccccatt
     1621 aatttttgga agcgattggt tccttttttc tcttcctgtt tctttgcata tttcaacgcc
     1681 gcatctagtt tttgacgggt tgtaatgatc acaacattcg cattggcatc tgccaacaga
     1741 gtttcactta actctttggc acttcctgtt cccacgatca agctattcgc tttggcatta
     1801 ccagaagaaa ccccagtatt aaactccgaa gcaaatttcg taaactctgt ggtggtctga
     1861 ttatctaaat ctttgcggtc cactaacatg atcgttcgat cgatccccga ttttcgtgct
     1921 aaaagcttag tagatacaaa actagtcaac gtctttcctg aaccagtcgc atgccacaca
     1981 taccctgatt gatgtttatt tgcggcaata aacaaggctt gaatcgcatg cacttgataa
     2041 ggatgtaaca ccatgagggt tttattgtct tgatcttcac tgacaatcgt ataatccgca
     2101 atcaagcgat gggcatctgg gatatttagc acttgtttgc aaaactcata taagttttct
     2161 acctttttat tatctttcgt tcgccaacta aataaaaact taggatgcaa atccttcggt
     2221 aaggcatttg caaaataccg agtcgtttgt tcattggaaa ccacaaacaa ctgtaaggtc
     2281 gaaaagatat tgttccgaaa catgccttct tcagcatatt ttttgatttg attaaacgcc
     2341 tgataaaaac catcttttgc agttacttgt tttagctcga tttggataat cggtaagcca
     2401 ttgatcaata aggtgacgtc aaaacgtcga tcccgcgctt ctatgttact tccacgtttt
     2461 gcaatttgat ggaccacttc ataagtagag attcctccac caatgtcttg attggaataa
     2521 aggataagtg agacagaacc caattgcgga ttttcccgtt caatcgtgat ccgagccatc
     2581 ccattttcac cttttaacca tttcgcagca tcaaatgggg ttttcgtccg tagcaataat
     2641 tcggttttga tcgtttcaaa ctctttatcg gtcaaaggag aatcattcag ttctgcttga
     2701 ttatttgaga tgattttttg acgtaaattg ctccaaagat cttcttctga ttttagatcc
     2761 ggacgatacg tccactgatt atgtccttcc ccgaggacac gaatcaactg ttcttcgact
     2821 tccgcttcct cacggtgact aattttcatt cttcttccct ccctaaacaa acatcttttg
     2881 caagaagcct tttttcgttt ccttcagcaa atctaactta cgttgatgaa gagcgatcgt
     2941 atcatctagc tgcttgaaga aggcaccaat tttttgttgt tcatttatat ccggtggaaa
     3001 ctgaaatgtc ctttgttcta taccaccttt agtcacatgt accattgtcg ttccgttagt
     3061 tgattcttta attttctctt tatcagtcaa tagccaagta tatagatact gtttgtccaa
     3121 ttccttagat aaaaatttaa gtttccaaat atgataatgg tagattacac gttcttcatt
     3181 ccacatttca ggaccgaagc ttgttgccca taaatataag agatctcctt tattagcata
     3241 cttgtcttcc tctaattcta agtttgaata ataccatcta tcattagtat taaaattacc
     3301 tactcttaaa accctatatt ttccagaatc aagtaattct tgttgtttaa aagctctacc
     3361 attcaaaaaa actaccactt cccccaactt ccgctcttcc caatctcccg taaatcctgg
     3421 aaaacgaact tctggcactt ttgctccgtt tttagggaac attttttgta agaatccttt
     3481 tttcgtttcc ttcagcaaat ctaattcgtg ctggtgaagc atgatggtgt gatcaagttg
     3541 tttgaaaaat gaaccgatct tgatctgttc gtcataagtt ggaagtgaga taggtgtctt
     3601 tttaccacta tcaattgtat aggttccgac ggttccaatg cctgcaacgt cacggatata
     3661 cttctgaatt ttgttattta tgaacgagta gtaaaagaag tttccgtcaa ttttatgctc
     3721 attcttgaac caaatgatgt ttccatcctt aaagtaaatt gggttatcat cagtgattaa
     3781 taaggggaca cctattgatc cgacaccagt tacaagcaag tctccttgag aaactttacc
     3841 tgaatttttc gaatattcat tatatttttc ttctgaaatg taaagataat cggatggttc
     3901 ctcatttttt gcagctgaga caatatcacg agcacgtaaa aatctaatac ctgagttagt
     3961 ccaatctgat tgatgaattc gcttcacaga agtaacatcc atcatatctc ctaacttacg
     4021 ctcttcccaa tcttccgtaa aaccaggaaa ccggatttct ggtcgtgtat cattgctcat
     4081 gtttgaacac ctccagcgca ccttgtaacc acgcttcatt ttcaggtgtt gtttgcaggg
     4141 aagagatagc ttctaaaagt tctttttcta gcacttgctg ttcttgtcgt atcttcttga
     4201 tgtcatttcc gacatgaacc atatcaattg gttcttcttc ttcgaaagta tcgacatagc
     4261 gtgggatatt taagttatag tcattttctt tgatttcatc aaaggttgcc aaatgagcgt
     4321 atttctcgac atcttttctt tcagcgtagg tttcgaggat tttttgaatg ttttcttccg
     4381 ataatttatt ctgatttttc cctttaacaa attcacgact agcatcaatg aataatacat
     4441 ctcgagtttg acgattcttc ttcaatacaa tcaccgtcgt tggaatcgat gtaccaaaga
     4501 acaagttcgc aggcataccg atcacagcat aaatactgcc gtcttctaat aatttttgac
     4561 gaatgacccc ttctgcagca ccacggaata aaaccccgtg tggcaagacg atagccatag
     4621 tccctgtttc cttcaaatga tagaaaccat gaagaagaaa cgcaaaatct gctttggatt
     4681 ttggcgctaa tttcccgtag cgattaaacc gagaatcatc taagaaggtc gtatctgctg
     4741 accaattagc cgaataaggt ggattcatca ctactgcatc aaatgtgtaa ggttcatctg
     4801 ttggccagtc cttattcaac gtatccccat tgcggagatt catttcttcg gcatctaccc
     4861 catgtaaaat caagttcatt tttgctagat tgtatgtcgt tgtatttagt tcttgcccat
     4921 gatatttaac gttatctgga tgagttaagt agttacgtac atttaacatc aaggaacctg
     4981 aacccatggt tgggtcaaag acactaaaga atcgtcgttc tttttgatcc aacgtcacga
     5041 tttgtgccat catatcagaa accatatgag gcgtgtaaaa ttctccggct ttcttccctg
     5101 cttctgaagc aaattgactg atcagaaatt catacgcatc cccgatcaca tccccatcgt
     5161 gacctaaaac ttccacgtca ttcaatttct tgataacttc ggtaatcgtc acatttcgtt
     5221 gttgctcatc tgttcctagt ttttttgatt ggagatccac atcatcaaat aacccattaa
     5281 attgattgta ggtagatgcc aattgcacaa aggctttgtt tagatcgttc aattggaacg
     5341 ttgcttgttt tgcttgatcc gctaagacgt tgaaaagata ctctggtgag atcgaatacc
     5401 ccaaaatatc aacgattgtg gcaatcaggt cttcttttga ttcttcatct gaaagtaatt
     5461 cacgatacaa cattgtttgt ttgcttactg tgtcatattc ctctaaggat tcatctgcta
     5521 aaagaaccac ttgttccaac aaacggtcgg ataaatattt atagaaaatc agtcccaata
     5581 aataattttt atattctgac gcatccattt tactacggag attatctgcc gcactaaata
     5641 atttttgatt taattctgca cccaaaaaga tacactccta tctcttattt aaagttgcta
     5701 tataccctct tattttagca tactcccttt tagaaaaagc aaaaaaaaaa gaaaaaatct
     5761 tattcttgat aacaagctac gtgacgtata tgcctaacaa aaatagaact tttgaatcaa
     5821 tcttaaatga aggtttaacc acagaaccgg catccattat ttttgatgaa gttatcgatg
     5881 acgttgaaga tagtaagtat cataaaaatt taaaagaact tttttatttt gcactcaaca
     5941 atggaccaag tttgttcact ggtatatcag tacctgatag tgataatcca ttaattatgg
     6001 caaaaattta cttaacaaaa tggtgtaata aatacatcag ggataggaat aacccagcat
     6061 taaaaaaacc tcttaaaaca tttggcgaaa aagatgctgc tttaactact agagtagcag
     6121 caaatgctaa tattgatgat caaaaagtat tgaatgacta cttgcgtgga cattttttat
     6181 atatgagcgc tgagaacatg aacggaagta ttttagagga atatttagcc gaagtgttag
     6241 aacctgaagg ttggatttgg tgcgccggct ctgtatatcg agctgttgat ttctgttatt
     6301 taggaacctc accaattctt ttacaagtga aaaataaata caatacagaa agcagttcga
     6361 gttcagctat aagagtagga acaacaatta gaaaatggaa tcgtttaaat aagtcgacaa
     6421 aaatttctgg tttggatagc cctatcccca attggaaagc attaatagaa atgactgaag
     6481 ctagtaagga actggcagct aagttaactg aaaatagtta tttagcttat attaatgaaa
     6541 aatccacacg agaattatgg acactcgatg actaacaata tccttattag tatctatcca
     6601 cgatacatgg aaaaaattat aaacaaacaa aaaaactttg aatttagaaa tttctatctt
     6661 acctcagatc cttataatat cttttggatt tacgaaacaa tccccaccaa agctttaaag
     6721 tataaattga ttgtaaaaaa tccaattact aaacttaaga aaaatcaaca ctacttttta
     6781 ggagacgatg agtttgaaca attagtttaa aag
//
LOCUS       DAHHZJ010000145         6804 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.147, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000145 DAHHZJ010000000
VERSION     DAHHZJ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.147"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(27..779)
                     /locus_tag="NBL02_002321"
     CDS             complement(27..779)
                     /locus_tag="NBL02_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCD2572994.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(1143..3809)
                     /locus_tag="NBL02_002322"
     CDS             complement(1143..3809)
                     /locus_tag="NBL02_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCD2572995.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            4150..5601
                     /gene="cls"
                     /locus_tag="NBL02_002323"
     CDS             4150..5601
                     /gene="cls"
                     /locus_tag="NBL02_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCD2572996.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            complement(5930..6745)
                     /gene="thiD"
                     /locus_tag="NBL02_002324"
     CDS             complement(5930..6745)
                     /gene="thiD"
                     /locus_tag="NBL02_002324"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCD2572997.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         1935 a         1450 c         1182 g         2237 t
ORIGIN      
        1 tataataaaa tcataaaaag aacccttcac gagtcgtgaa aggttctttt tttatctgcg
       61 gaccaaagga gcagtctata ggtcagctta gtcgtactaa ggatttctcg tttttcactg
      121 aatagtttgc gaatgtacct ggtcacaaca ggatcttttg cgtacgcgtc atagtatagc
      181 ttcatcaatt cttcagtaat tgattcctgt gtaaaataag taaaatcttt tttcttcact
      241 ttgaatcctt catcaattaa gtatgtttca aattgatcca ttacaactgg cgatgtatta
      301 ggatcttctt ctatcattcc tagttgcttt tcgataggat aaaaaacatc atcgattgat
      361 tccactactc tgattatcac gcaccatttg cttgactttt tcatcaattt ttctaattga
      421 ataagatgta ccaatgctgg atttgctgct gtaaaaacca tttcatatgt ctcatttgat
      481 tggataaatt cttctaacga ctgtttgata aaggaaacat ttggcaactc atttttttct
      541 gcttcttttt ttgcattttc tagcattcct gaagaaaaat cgatcaaatc atagcgatta
      601 atttcttttg ctaaggaaag aaatctgcca gatcctccag caacgtctgc tgctgtttca
      661 gcaggcagga ctttctccgt tttcagataa ttttttacat cttcagcaat agttacttgc
      721 gattcttgct ggatttcata atattcttca gcaaattcat cccattcttt ttcattcatt
      781 tgatttcctc taattttttg ttatatgttt tcacttttcg gaataaatca ttctcctctt
      841 ttatctattc tatctctttt gatgaataat aaaatgattg gctttccgtc tatcataaat
      901 aaaaaaaggg actgtgacca aagtcgtgtc aaagtccctc tttccgaata aacagtgtcc
      961 aatcagatga ccctcagtat tagctcaaaa tgctgatttt acaacttctt ccttaaacgg
     1021 tgttctcctg ccaactcttc ggtcacaagt cacaatattc aacaaacatg agaagctgtt
     1081 tgttgaaatt gcaccttgtg actcagaatt aaacggcagg ctcacaacct cgttgattcg
     1141 ttttatttat ttccttgtac tcgtacaatg atcaatttag tgatttccat caaaacaatt
     1201 gtcacaagtg cgattcccat acatgtaaag acttctgtca atccaaatgc tgatgggata
     1261 gagaagactt ctttcagtcc tgggatcatt gttaagcagt aaagcgcgct acatacaact
     1321 acagctagta agacacttct gttagagaag aaccctgctt ggatagctgt ttgtgtgttt
     1381 gaacgtgctg ggaatgtttg caatgtccga caccaaatca atgttgaaaa tgacatggct
     1441 acaccgaatt caggtgaatg ctgcatgcca agccattgag caaagataac agcaatccca
     1501 atcagcacac cacgatacgt tacagaaatc aatgttttgt ccgtaaagat tcctgaattt
     1561 ggatctcttg gcgggcggtt catcgtagca ggttcagctt tttctgtacc taatgcaatt
     1621 gctggcagtg aatcattgac taaattgatg aacaacagtt gtaatgctgt aaatggattg
     1681 gaccagtcag caactaaggc aaagatgatc gcaatgatcg cacctaaatt tcctgcaaat
     1741 agataagaaa tagctttctt gatattatca aacacattac gtcctacttc tactgcgctg
     1801 acaatagaag caaagttatc atccgtcaat accatcgctg ctgcatcttt tgccacatct
     1861 gtacccgttc ccatagcaat tccgatgtct gcttgtttca atgctggtgc gtcatttaca
     1921 ccatcacctg tcatagctga gatatgacct ttattttgcc aagcacgaac gatacgaatt
     1981 ttattctcag gtgaaacacg tgcataaaca gtgacttttt ctaaaacttc gtttagttga
     2041 tcttctgtca attcatctag ttccgtcccg gtcaaagcca ggtttccttc ttcaaaaatc
     2101 ccgatttgtt ttgcgatagc aacagctgtt gttttgtggt caccagtaat catgattgtt
     2161 ttgattcctg cacttcttgc ttctgttacc gcctgagtca cttcagcacg tggaggatcg
     2221 atcatagcaa gcaaaccaac aaggatcaaa tcgtcttcat cttcaaatga taaatcaaaa
     2281 ttatcaagtg gacggtaagc aaaagctagt acacgtaatg cgcgtgttga aaaagcttca
     2341 ttttgctctt ggaatttttt catcaattca tccgtcatcg gaacgacttc accatttagc
     2401 aatacttttt tactgcgttg gaagacgata tctggtccgc ctttaacaaa caagtaattt
     2461 ttgttatcaa ttgtgtgtcc agtagacatt aattttcgat ctgagtcaaa aggaagttct
     2521 gcttgtcttg ggtagttgtt tcggatttcc tgataaggct ggtttaattt ttcactaaag
     2581 tcgataaacg ctacttccgt agggtcgccc aatttagtac cgtcttcact gatcgatgca
     2641 tcattcgcta aaacactgat ttcgatcaag cgacgttcgt cagctgtcca ttttgaaggg
     2701 tcatttgtga aatccccagt atcgccatta cctaaaaagt agtcaacaac tgtcattttg
     2761 ttttgtgtca atgtaccagt cttatctgta cagatgatac ttgtcgaacc aagtgtttca
     2821 actgctggaa gcttccgaat aatggcatga cgttttgcca ttttattcgt tcccaaagat
     2881 aacacgattg ttacgataga ctgtaatgct tctggaatag cagctacggc tacggctacg
     2941 gcaaacatca ctgcgctgac gatatctgca gtcatatctc ctgtaccttc tccaagataa
     3001 atacgaaaca gttggatacc taaaatcaat aaagacaatg ccaaaatagc caaacttagt
     3061 tttttactga atttatccag acctcgttgt aacggagtga cttgctcagt tgttgattcc
     3121 aaaaggccag caacttgtcc gatctccgta ttattcccag ttgaagtcac taagaattta
     3181 ccgcgtccgt aagttacgat cgttccactg aataccatat ttgtacgatc tccgattggt
     3241 accgtacttg tgatatccgt tacttctttt tctgcagaag tcgactctcc tgtcagcata
     3301 ccttcatcta cttttaatga tcccgcatct agtaaacgtc catcagcagg tacataatcg
     3361 cctgcgtcta acaacacgat atctcctacc acaagttcgt ttgccggaat cgttttttcc
     3421 ttaccatcac gaatcactcg agcatcgggc gctgaaaggt ttttcaacgc ttctaaagaa
     3481 ccttctgctt tttttgtttg gacaacactg attatagaat tcaacatcaa tactgcaaaa
     3541 ataactaaga attcgatgta gtcacccatg accatctgaa tcagtgcgac aatcagcaat
     3601 acgataacca ttgcatcttt gaaagtttct aagaacaatt tccaagttgg cttcttcggc
     3661 ttttctttta gctggttcaa gccatctctt tctaattttt ccttcgcttg cgtggtgctc
     3721 aatccatcgg ttgagctctg cgtttgctgc atcaacgagt cgatcgtttc tttatagacc
     3781 gcttgttgtg cttgttcctt gttttccaaa tttcatcccc cattttttga tcttggccct
     3841 agtcctgtct gaaaaaaaag agacctaaga caaatacgca tttgtcttag gtctcaccat
     3901 ttcatacaat accagaaaac catttggtta acttactgtt gatattatcg caacaaacgt
     3961 tgccggttac tcccctaagg agatattcaa ctgtactaat atcctacctt attaacctac
     4021 aaaaagcaac tactttagcc cgatttacct acattttcat gaaaggagtg aaaagtatat
     4081 tcaccttggt aggcttcttt cttctatcat gctatactaa tgttattgta tgtaaggagg
     4141 aacatttttg tggtatctag tattataacc gccctttatc tattaaatgc acttattgct
     4201 ttggtggcta ttttgatcaa accccgagat gtagcagcca tttgggcatg gctcttagta
     4261 ttgtttgccc ttcctggggt gggctttgta ttatatttat ttttcggacg tggattaacg
     4321 gataagaaaa aattctatct ccgacaaagt gacttgaaag aattagaaaa ctttcagtct
     4381 tttagaggag ataccattga acattacgat cctgacatgg gcgataaaga caaacagcaa
     4441 tttgttgact tcttctcttc attaaatcgt atgccgctga caagaatgaa ttctgtcact
     4501 cttctcacag acggacaaga gaaattggat tcactgcttc aagatctaaa aaaagccaaa
     4561 cattcgatcc atatcgaata ttacgcattt gtgacagata atatcggcca gcaagtctta
     4621 catgttttag aagaaaaagc cgcagaaggc gtggaagttc gaatattata tgatgcattt
     4681 ggctctcatg gcacaaaagc aaaagatttc aatcgtctaa tcaaaaatgg tggacatgtc
     4741 catacatttg ttacctcaca aagggcatta cttcgtttcc gattgaatta ccatgatcac
     4801 cgaaaaatcg ttgtgatcga tggaaagatt agttataccg gtggtttcaa tattgccaat
     4861 caatatgtaa atacaacaaa aaaattcggc tattggcgcg atacgcatat acggattttc
     4921 ggtgccgctt cttctttgct ccagcttcgc ttcttaacag actggaacgt ctcggtacct
     4981 gaagaaaaaa aggtcggcta tcatttgaat tatttcttta aaaaagcaga tcgagatgaa
     5041 tctaagcttg ctgatacatc catccagctt gtttcaagcg gaccgaataa cgaaagggaa
     5101 caaatcaagc tttcatttat caaattgatt acttctgcta aaaaacgtgt ttggatacag
     5161 acaccttacc ttgttcctga tgaaagtgtc attgctgctt taaaaatcgc aactgcctct
     5221 ggtgtagatg tgaaaatcat gattcccaac aaaccggatc atccttttat ttatcgagca
     5281 acacaatatt atgctcggca gctgatcaag gaaaatgtac aaatccttgt ctatgagaac
     5341 ggcttcctcc atgcaaaaac attgataatg gatgatgaaa tctgcatggt aggttcagca
     5401 aatcaagata ttcgtagcta ccgattgaat tttgaaacaa gtgctgtcat ttacgatcct
     5461 gagtttttag aagaacttgc tactcagttc aaagaagatg agacacattg ttcatccatg
     5521 acaactgaaa cagtcaagga aatgtctaac tggctattat tcaagcaaca aatttctcga
     5581 ttattttctc caatcctata atttttttat tcaagtagag aaaaatatac taagattaaa
     5641 gggactgtga caaaaatctt gtcacagtcc cttcttccga acaaacggtg ttctcctgcc
     5701 aactcctcgg tcacaagtca caatattcaa caaacatgag aagctgtttt ttgaacttgt
     5761 tccttgtgac tcggagctgg acggcaggct cacgacctct ttcttaaacg gtgttcaaaa
     5821 gcttgcatct gcggtaataa gtccaaccaa gttaaaaaca taagaagctg tttttacttg
     5881 gctgttctta ttacttgatg cagggcaact ttttcacaac ttctttcttt tactctaatt
     5941 caccagctgc ccaatgattg atggggccat atttgtgccc tacatttatt tcttcacgaa
     6001 ttgcacggtt tacatagtct tttgccaatc ggatcgcttg ttcaactgtt tttcctttcg
     6061 ctaactcagc tgcaatacat gcagatagcg aatcgccagt tccgttgatc cggtcggtag
     6121 gataaaatgg tgcatttaac caaaatgatt ttcctgattc gaacaatacg tagtcattga
     6181 ctgtttcttg ctgcgcattt ttagcatgat gtcctttgat cataacgttt tttgcaccca
     6241 tatcttgcag ttcttttgct gcttgcacaa aatcttcttc agctgctaat gtccgtccag
     6301 tcagcttttc tgcttcataa aaattaggtg tcagtacttc ggccaaaggg acaagctttt
     6361 ctttcaagct ttgcaaagca gattcttcta gtaaaagatt tccatgtttc gttacgatta
     6421 cgggatccac gactaatggt ccaaaatcat atttttggta attttttaca actgtctcaa
     6481 tcaactttga atctgctagc atacctgttt tagctgcacg tatttgaaag tccttcgcaa
     6541 tcagttcaaa ttctttgtcg ataaaatctg ttggcaatgt cacacttgct ccgattccgt
     6601 aagaattacc tgcaacacat gcggtcatga cagaaacacc gtaaacattt cttgtaaaga
     6661 atgtatgcat atctgcttgc atccctgcgc ttccatcgct atctgatcct gcgatcgtca
     6721 ttgcttgtac atactgatct tccatcaaat cgattcctcc atttttataa aaagattttt
     6781 cataatactg ttactgtttc tatc
//
LOCUS       DAHHZJ010000146         6549 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.148, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000146 DAHHZJ010000000
VERSION     DAHHZJ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.148"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            119..3346
                     /gene="fss3"
                     /locus_tag="NBL02_002325"
     CDS             119..3346
                     /gene="fss3"
                     /locus_tag="NBL02_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HCD2572998.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYCQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            3459..3773
                     /locus_tag="NBL02_002326"
     CDS             3459..3773
                     /locus_tag="NBL02_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCD2572999.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3786..4160
                     /locus_tag="NBL02_002327"
     CDS             3786..4160
                     /locus_tag="NBL02_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCD2573000.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            4170..4505
                     /locus_tag="NBL02_002328"
     CDS             4170..4505
                     /locus_tag="NBL02_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HCD2573001.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            4588..5928
                     /locus_tag="NBL02_002329"
     CDS             4588..5928
                     /locus_tag="NBL02_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HCD2573002.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            6006..>6549
                     /locus_tag="NBL02_002330"
     CDS             6006..>6549
                     /locus_tag="NBL02_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HCD2573003.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPD"
BASE COUNT         2197 a         1104 c         1449 g         1799 t
ORIGIN      
        1 tcattcattc tcaaatccat tctattcagt caataaaaat tttaaaaaga ctttataaac
       61 aaagatgttc aagcgagcat cttatttttt tgtgcttaat ttgaaaggag aacatttcat
      121 gtataaaaaa caaaaaaaga aattatgtgt attaactatt ttgagtttgg ctgttcaagc
      181 aattgttgga gcaattgctc ctacgattac ttttgctgat gaaattaccc atccacaaac
      241 ggtaaccgta gaactagatt tagcacatca atatgctgtt gaaggaactt ttagtgatgg
      301 tcgtccaatg tcagaagtca ctgttccgca ttatgcagtg tacaacggct ttaaacaaga
      361 tattttctgt attgaaccag gagtcccgat ttacaatgag ttcactcctg gctatgagaa
      421 aaacccgtta ccagatatgt ccgataaagc caaattagtt tccgttttat ggaaaaaagt
      481 aggtacagat gcagatactc atattgtggc acagaagatg atctggcaag aagttaatgg
      541 gtataccctt cactcaataa aacgatcaga tggtagtgcc gtaaatatcg cagccattga
      601 ggcaaaaata aatcaagcca ttgacgatta tcagaagaaa ccaaccttcc ataatagtac
      661 ggctaaaaca gtattagggc aatcgactac tgtgacggat acgaataatc tgaatttgtc
      721 agagttcgat gaggttgtgg aaaatgccgc aaacattgat tatcgtgtga atggtaatca
      781 gttgataatc accccaaatg ccaattcaaa agaaagtggt gtgctgacgc ttaagaaatc
      841 tgctggtacc ggaacacctg tcgcctataa aaaggctggc caacagactt taatggccgg
      901 ggcgattgat aaacctaata cctacacaat caaaattgat gtggaaacgg agggttcttt
      961 aaagatcaaa aaagtcgata aagaatcggg tgatgttgta ccaggaacgg ttttccattt
     1021 agactttgga aaaactttac ctgcaaaaga cgtgactact gacaaagaag gcattgctac
     1081 attggatgag attcctcatg gtacaaaagt aaccattacc gaaaaatcag taccggcgcc
     1141 ttatacgatt gataccaccc ccatgactac caccattaaa gcaggcgaaa ccatttacgt
     1201 gacttcaaaa aacgcacggg aaaaagggca aatcattctg gataaaagcg gagttgaaac
     1261 agggagtgat ctttggaatg acaattattc cttagctggt aatacgtttg ccattcgcaa
     1321 agacagtcca accggtgaaa tcgttcaaga aatgaccacg gatgaaaacg gtcatgcgga
     1381 aacaccaaaa gagattgcta atgcgttgga attgggaacc tattatgtga ccgaaactaa
     1441 agctagtcat gggttcgtga atacgttcaa acctgtaaaa gtcgaattga agtatgccaa
     1501 tcaaaccgta gcccctgtta ccagtaacgt aaaagggcaa aatcaagaag tcactggcga
     1561 aaccactttg acgaaagaag acaaagatac tggggataaa gcacaaggta aggcagtctt
     1621 tgagggtgct gaatacacct tgtttactgc taaggatgga aaagctgtta aatggagtga
     1681 ggcttttaaa ccagagttag cgaagggaac gaaaggttct gatgaaacgg tcactttgac
     1741 tttagacgaa aagaaccaag ttgccgttaa acatttagct atcaacgagt attattgcca
     1801 agaaacaaaa gcgcccgaag gatatagcct tgatgaaaca aagtatcctg tatccatcaa
     1861 aaaggtggat gatagcgaaa aaaatgccgt gattacccga aatgttacgg caaaagaaca
     1921 agtgattcgc tttggctttg atttctttaa atttgctgga tcagctgcgg gcactaccga
     1981 aactggcttc aatgatctga cctttaaagt gtcgccattg gaagggacca atgaaatcac
     2041 aggtgccgaa gatgaagcga ccacagctta taacgagcaa ttagggtttg atggttatgg
     2101 caagtttgaa aatcttcctt atggggatta tttacttgaa gaagtagaag ccccagaagg
     2161 ttttcaaaag attacgccat tagaaatccg ttctactttt aaggaaaaca aagaagactt
     2221 tgtgaagagt gagtatgtct ttaccattac cgaacaagac caaaaacaac caattaaaac
     2281 ggtgaccgtt ccttacgaga agctgacgaa caaagcattt tctgttagtt tgaaccgttt
     2341 aatgctctat gatttgcctg aggaagaaga tagtttaact tctcttgcga cttggaaaga
     2401 cggaaacaaa gaattaactt ctctggattc taccgagcta gttgataaat tgagttacaa
     2461 tctacatgaa atcaaagaag actggtatgt ggtagcgcaa gccattgatg tggatgcgac
     2521 aaaagctgcc caagaaaaag atgaaaaagc caaaccagtg gtgattgcag aaacaagcgc
     2581 aacgttggcg aacaaagaga aaacaggaac ttggaaaatt cagcataaat taaccgccga
     2641 acaagtttta aataaaacta ttgtcttatt caactatgtg tatgaaaata aggaagcttt
     2701 tgaagcaggc gataagccag tagcgaagga tgttagcttg aataaccaag cccaaactgt
     2761 cagttgtaca gtggaacacc atgtttctat ccaaacaaag gcccacctag aaaacggttc
     2821 tcagaccttt actcatggtg atgtggtgga tatgtttgat gatgtgtcga ttactcatga
     2881 tgtactggat ggctcaaaag aagctttcga aacaattctg tatgctttat taccggatgg
     2941 tacaaacaaa gaaatttgga aatctggcaa gattgattat gaagtaaatg ataaagaatt
     3001 caccaaaact gtgcttgcga aaaaagtaga taccggaaaa tatccagaag gaaccaagtt
     3061 tacttttgcg gaaatcaatt acgataaaga tgggaatatc aatggaaaac acaatgaaga
     3121 tttgaaagaa aaatctcaaa ccttaacacc aaaagaagtt ccaaccatac taagtacgcc
     3181 gaaacaaccg gaaacaccaa ctgttccaag tggctctcaa gaatctagtc ccacagtgaa
     3241 gaccttccct caaaccgggg agaaaaattc caatgtttta ctgttcattg gttttacttt
     3301 gatttttgcg acggcgggct attatttctg gaatcgccgg aactaaggtg atgagatgat
     3361 acaagtaaat cctaagaacc aacgtgcgcg accgcctcct aaaaaagtag tcgcgctatt
     3421 tttgtatcca aaactaacaa tgaaaagagg aaaagaagat ggaattgaaa tttgtcgtgc
     3481 cagatatggc tgaaaccttt ggaaagttaa gttatgccgg tgaaggagaa attataacag
     3541 aaggatatgg acggaatact acggtgattg gtcgcagtta tcatttgtat tccagtaagc
     3601 aacgggccga tgatattgag gtggtagtag ctgcggaagc cggtgaaaag gattttgatc
     3661 aagaccaacc gctaaaagcc gtgaatcccc atttggttgc caaaggctat gagattgaga
     3721 atcgtgggtt tactgattac gtgttgtatg ttgatgattt agtgaaagca taggaggaaa
     3781 agaagatgag attaacagaa ggtattgtcg tagattcagg tttaacattt gggaagttgc
     3841 gtttttccgc attacgccga gaagtacgga agcaaaatga ggatggaaca attggtaatg
     3901 aggtaaaaga acgaacctat aatttaaaat cctctgcaca agggcgaatg attcaagtta
     3961 gtatcccagc taatgtaccc ttacgcgagt ttgcttatga tgcagaggta gagttggtca
     4021 atccaatcgt ggataccgtt gccaattatg tttttcgaga aggaactacc gttaattggt
     4081 tcattaaggc ggatgattta gtcttgaagc ggcagccgaa tcaaggaaat cctacgaacc
     4141 aaaatgaaag aaagaaatag gtgaagatta tggaaattaa tgttgaacat attttagatt
     4201 gtctcgatca atatggaaaa ggcgagctga ctgaggaaca actcgttaaa gcattaacct
     4261 ctgatgaaaa aatgtttctg attatacatc aaggtttact tggagaaaat agcgacagtg
     4321 aagaagactc tgatgtacta gactggttag gagaagaaga gtctttcttt atagttgtcg
     4381 aagtaaatga aaacttatgt cggcaggcag aatccatttt ggaagaaatt ggtgtggaga
     4441 tgccggatgc tattgaaggc tttttaaatc aattggttga aacgaaacaa ctcccctcct
     4501 cgtagcagta aatgactagc ataacttttt gaaattgcct aatgaaatcc tattgggctt
     4561 tttcgattgg aggtggaaac catcaaaatg tacaaaggac atcgcatacg cgcaggagat
     4621 cagcacttgg tttatcactt tgttcttggc tggctactcg ctctgttcat cggttggatg
     4681 agcgtctttt attttcaaga gtttagacaa tttgatattt ctaagctatc actttctact
     4741 attgaaatcg tttggtctat aaaagattta gtatgtctgc ttggaagtct cgctttttca
     4801 ggagctatga tattgcttta tattcacttt tttcttgatc attggcgaag cttgtggcat
     4861 cgacaaaagc tagctcggat gattctagag aaccattggt atgaagtgaa acaaactcaa
     4921 agtgaaggct ttttcaaaga tttgaatagt agccgaacca gagagactat cagttacttc
     4981 cccaaaatct gttatcgaat gaaagatgga ttactgtcta ttcgcgttca aatttcactt
     5041 gggaaatacc aagatcagct tttaaaattg gaaaagaagt tagaaagcgg actgtattgt
     5101 gagttagtag aaaaggaact caaagactcc tatgtggagt atactttact gtatgatatg
     5161 atcgccaatc gaattgggat agacgaagta gtggcagaaa atgggactct gcgattgatg
     5221 aaaaatcaag tatgggctta tgattcttta ccccatatgt taatcgctgg tggcacgggt
     5281 ggagggaaga cctatttcct cctcaccatt atcgaagcac tattgaagtc agatgcagaa
     5341 ttgtttatcc tcgatcccaa aaatgcagat ttagctgatt taggtacggt gatgcctcat
     5401 gtttattcac aaaaggaaga aatttctgct tgtgtggaag atttctatga acgtatgatg
     5461 gctcgtagca aggcaatgaa agaaatgtct aactacaaaa caggagagaa ttatgcctat
     5521 cttggacttc cacccaactt tttaatcttt gatgaatacg tggcttatat ggagatgtta
     5581 acgacgaaag aaagtgcggt gattttgaat aagctcaaac aaatcgtgat gttgggtcgt
     5641 caatctggtt tctttctgat tttggcttgc caaaggccgg atgcgaaata tttaggagac
     5701 ggaatccgcg atcaattcaa ctttcgcgtg gctttgggtc gtatgagtga actcggatat
     5761 tccatgatgt ttggggaggt cgaaaaaaac ttctttatga agcacatcaa aggtcgaggt
     5821 tatgtggata caggtggcag tgtgattagt gagttttata cgccacttgt accaaaaggg
     5881 tatgatttct tagattcaat tagtaaatta gaaaagatga atgattgagt gatcttcaat
     5941 actgacttta tacttcaagt aacgtataat acttttatat agtaggaaga aaggggataa
     6001 ttaatatgga aagtaatgat tcttatttag agtatgcaaa agcaaataag gataagttta
     6061 tttcttatat tatcaaaggg aaatcccccg aagttggacg agacgcagtt tttatggcag
     6121 ggagtcctgg ggctggaaaa tcagaggttg ccattggatt agctgagaat tatgataacc
     6181 atgtgattat tgatgcggat tactttcgca cccagtttcc tgattataat ggaaaaaact
     6241 ctagtctctt tcaaaaagca tcttcttggt tggtagagca ggcatttaga tatgtcttag
     6301 atcatggcta ttcttttatt ctggatgcaa cttttgcaat attaagtgct gaaaaaaata
     6361 tctcgcgggc gttgaaaaaa cattttcgag tgacgatttt ctatgtttat caagacccta
     6421 aaatcgcttg gaaatttaca caagaacgtg aaattgcaga gggacgtcat gtgccgaaaa
     6481 aggcatttat taacgctttt ttcaaatcaa gagagaacat tgcaaaagta aagagtaaac
     6541 atccagatg
//
LOCUS       DAHHZJ010000147         6502 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.149, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000147 DAHHZJ010000000
VERSION     DAHHZJ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.149"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            132..1763
                     /locus_tag="NBL02_002331"
     CDS             132..1763
                     /locus_tag="NBL02_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCD2573004.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            1766..2845
                     /gene="uxuA"
                     /locus_tag="NBL02_002332"
     CDS             1766..2845
                     /gene="uxuA"
                     /locus_tag="NBL02_002332"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCD2573005.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            2919..4346
                     /gene="uxaC"
                     /locus_tag="NBL02_002333"
     CDS             2919..4346
                     /gene="uxaC"
                     /locus_tag="NBL02_002333"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCD2573006.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            4430..5077
                     /locus_tag="NBL02_002334"
     CDS             4430..5077
                     /locus_tag="NBL02_002334"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HCD2573007.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            5197..>6502
                     /locus_tag="NBL02_002335"
     CDS             5197..>6502
                     /locus_tag="NBL02_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD2573008.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLIL"
BASE COUNT         2206 a          870 c         1345 g         2081 t
ORIGIN      
        1 ataaaaaata caatcattct gatatggtag aaaaaagcaa gtttattccc tttttatatt
       61 gacaacggtt acaatgggtt ttaaaatata aaatgtatca aaactaacat atcagtttga
      121 ggaggttaat tatggtttcg attaaaaatt ggcaaaattc tatcgaagaa ttcaatgaaa
      181 tgcagattct actgccgtgt tttgatgtca aagaagttaa gcaagaaggc cagcaacatc
      241 cagtatggct tcattttggt ggaggaaatc tttatcgagg atttcatgca gaaattgctc
      301 aatgcttagc agatcaaaat gaattagaaa caggagtagt tgtgtgtgaa acatttgatg
      361 aagaagtagt ggaaaaagct tatcatgact ataacaatga cattttggaa attgttatga
      421 gtggagatgg tgaactcaaa aaaagacttt tggcagtaac ggctgatagt ttatattgca
      481 atccgaagaa tatagcggac tatcaaagag tggttaaata ttttgaagat cctaaattac
      541 agtttacaac gtttactatt actgaaaaag gttacagttt aaaagattct aaagggcaat
      601 tcttttcggt agtcaaagct gatatcaaat ctggacctag caatcctcaa catacgatga
      661 gtattgtaac atccttgtta tatagcagat ttaatgctgg agagttgccg attgcgatgg
      721 tttctactga taatttctca cagaatggtc aacgttttca agatagtatt tttacgattg
      781 cagaagcttg gcaaaaagaa ggatatgttt ctgaaaaatt tattgcttat ttaaaggata
      841 aagaaaaagt gacttttcct tggagtatga ttgatcgaat tacgccaaat ccttcagaga
      901 atgtaaagga agatctattg atgatggggc tcgaggattt agatatcatt cacacaaaaa
      961 aacataccaa tattgctgct ttcactaata ctgaggtagt tcattattta gttattgaag
     1021 atagttttcc aaatggccgg cctaaattgg aaaaggctgg tgtgattatg acagatagag
     1081 agacggttga taaagcagac atcatgaagg ttacgacaag cttgaatcca cttcatactg
     1141 ctttggcagt aacaggttgt ctgctaggat atcaatcaat ttcagcagag atgcaggatg
     1201 atgatttggt tggtttaatc aaaggaatag gttatttaga aggtttacca gtagttgaaa
     1261 atccaggcat tattgatcca aaacaattta ttgatgaagt aattaataaa cgtttgccta
     1321 atccctatat tccagataca ccacaacgaa ttgcaacaga tacttctcaa aaagtagcga
     1381 ttcgctatgg tgaaacaatt aaaaagtatg tcgaaaaaga cggaaatgca gaaaatctaa
     1441 catttattcc tatcgctatt gctgcttggt tacgttattt actggcagtc gatgatcaag
     1501 gggaaaattt tgtgccaagt cctgatccgt tattggtaga gttgcaggat aaattatcag
     1561 tattgaaatt aggtgaacaa gatccttcaa agattcattg tgcggtacag gaaattttag
     1621 aaaataaaga aatctttggt ttagatttat atcaagttgg ggtaggaaag aaaactgaaa
     1681 aaatcttttg cgaaatgcta gtaggtcctc aagcagttcg taaaacaatc aataaatata
     1741 taacagaagt tggaggaaag taactatgga aatgactttt agatggtatg gggcaaatga
     1801 agaaaaaatc tcactacaag aaattcgtca gatcccagca atgaaaggaa ttgttggaac
     1861 attaatggat ataccagccg gagaggtttg gccgagagaa cgaattaaat ctttaaaaga
     1921 tgaagtcgaa gccaatggac ttaccttgaa agttattgag agtgttaatg ttcatgatga
     1981 tatcaaaatt ggtttaccaa cacgagatca atacattgag aattataaac agacgattcg
     2041 caatttggca gaatttggga ttgaggttat ctgttataat tttatgccag tatttgattg
     2101 ggtaaaatcg gatttggatt atcgtttaga tgatgggtct tctacgttag cctatatttc
     2161 aaaaaatatc cctactgatc cggaggaaat tattagagaa gtagaaaatt ctactaataa
     2221 ttttgaattg ccgggttggg aacctcatcg gttatcacaa attaagcaat tattagaagc
     2281 ctataaagaa gttgatgaag agaaattacg agagaacttt gcatattttc tcaaagcaat
     2341 tatcccgaca tgtgaagaag ttgggattaa aatggcagtt cacccagatg atccaccata
     2401 ttccattttc ggattacctc gtattgttaa aaatcgtgac gatttagatt ggttgtgtaa
     2461 tgtagtggat tctccttcta atggtattac attatgtact ggaagtattg cagaacgacc
     2521 agataatgat gtctatgaaa ttctatctga attttgtcaa cgggatcgga ttccttttgc
     2581 acatgttcgt aatatcaaat atttaagtga caatggactc gatttttacg aagcgccgca
     2641 taaatcagaa tatggttctc tagatatgta taagatatta aaaacaatgt acgataatgg
     2701 atttaatggc tatattcgtc cagatcatgg tcggatgatt tggggagaaa ccggtagacc
     2761 tggttatgga ttatatgacc gagctctagg tgcagtttat ttaaatggta tttgggaagc
     2821 gcttgaaaag ggggcgagta aataattata taagggagtt tatttagagg aaaaattttc
     2881 tcttctaaaa actaacggaa ggaaaggagt gtttaaatat gtttttagat aagaactttt
     2941 tattagagaa tgaaatagct aaaagacttt ttcataaatt tgcaaaacac caaccaatta
     3001 tcgactatca ttgtcattta gacccagcat atttgctaga aaataagcaa tacaagaatt
     3061 tgactcaggt atggttaaat gataaaggag ctggagatca ttataaatgg cgattgatgc
     3121 gagctaatgg tatagaagaa gagttgatca gtggtccagg tgatgattat gaaaaatttt
     3181 tagcttttgt aaaaacagtg gagaaggcgc caggtaatcc tatttttgaa tggtctcatt
     3241 tggaattaca acgttacttt gatattgatt tgacaatttc tgagaagaat gcaccagaaa
     3301 tttggcgacg tgctaacgaa aaaattcaaa cggattccta tcgcccgaaa gaattgttgg
     3361 agatgatgga tgtgaaagcc atctgtacaa cggatgatcc tacagatgac ttgtggtatc
     3421 atactgagct ttctaaacag gataattctt tcaaggtatt acctactttc cgtccagata
     3481 aggtctggag tatcaaatca tcaggctttt cgaactatat tttaaaactt agtcaaacag
     3541 cggctatacc aatcaaagac tttaatgggt tgaagcaagc attgagccaa cgagtggatc
     3601 attttcatca aattggctgt cgattagcag atcacggttt gaatacttat ttttatgaaa
     3661 tagcagatga aaaaatactc gacaatctgt taataaaagc tttagcagga aaaacggatt
     3721 tccatttatc agaaattgca caatttgtta caggtattca attacatttg atgtctgagt
     3781 atacaagata tggttggacg atgcaaatgc atttaaatgc tttaagaaat aattcaagta
     3841 tattaaagga acaaattgga gtagatgtcg gtggtgattc gatgggggat caagctagtt
     3901 tgacacaaaa tgttgtagca ttgttcgata ccgctgaaaa gagtgggtgc ttaccaaaaa
     3961 caattttata ttcattaaat ccaactgatt ggcttccact agcaacagca atgcaaagct
     4021 tccaaggggg tatgattcaa aaaatccaat tgggatgtgc ttggtggttc aatgatacag
     4081 ctgaaggaat gcgtcaacag ttgactacga tggcacaaca aagtttgttg tcgaatttta
     4141 caggtatgtt gactgattct cgaagttttc taagctatcc tcgccatgag tattttcgtc
     4201 gagtattatg tcaattacta agcgagtggg ttaaagcagg ccgaattcca gatgatgaaa
     4261 tgtatcttag tcagatagtg gagaatattt gttatcgtaa tgcgtataat tattttaatt
     4321 tttttgaaat cggaagtaga aaataatact tgaaataaaa gcttaaattt attctttgta
     4381 agttagtaca gctagacaaa gtatgtatcc ataaaggaag gagatagata tgaaaaaagt
     4441 agaaatttta agtaagttag aagcggctgg agtcattgcg gttgtacgtg ggaaaaataa
     4501 ggatgaggcc attaaagcaa taaattcaat tattgcgggt ggaattaaag ggattgaact
     4561 aacttttaca gttcctaatg ctgaagaaat aatcaaagaa attgctaaaa aatatgctga
     4621 tcaaaaagat gtaataatcg gagccggtac ggttttagat gcgtttactg ctcgtatcgc
     4681 aattatggct ggggcagaat atatcgttag tccatctttt gatcgacaaa cagctgagat
     4741 ttgtaattta tatcaaattc cttatttacc aggttgtatg acaattactg agataaagga
     4801 ggcacttact gctggcgtag atatcattaa actattccca ggttctgctt ttggtccatc
     4861 cattgtaaaa gcctatcaag ctccattacc acacgtaaat ataatgccta ctggcggagt
     4921 aagtatagag aacttagaag attggtttga agtaggagtt gttgcagttg gtgtaggtgg
     4981 gaatttattg gcttctgttg aaaaaggtga ttttgaagaa gtaagtcgag tagctagtaa
     5041 atatatagat aagttttatg agataaagaa ttattaggag aaaaaacaaa taatactctg
     5101 aaatgtaaaa aataaatact gactctttac gcttcagagt atagtacgta ctttcatcag
     5161 aatgaaaacg cttaacaaca gaagggagag aattgtatgg aaagaaaatt tggtataaga
     5221 gacaaaatag gatatatgtt tggagatttt ggaaacgatt ttactttcat ttttgctagt
     5281 tcttttttaa tgatctttta tacaaaagta ttggggataa gcggagctgc cgttggaaca
     5341 ttatttttag tagcaagatt tatcgatgca gttactgatg taactatggg gcgtattgtt
     5401 gatagatcga aagcaactaa gcagggtaag tttaaaccgt ggattttaag aatgagtggt
     5461 ccagtcgctt tagcaagttt tttaatgtat caaacttcta tgatttcagc ttctatgagc
     5521 gttaaggtag tatacatgta tgttacgtat atattatggg ggagtatttt ttacacatct
     5581 ataaatattc cttatggatc aatggcatca gctattactt ctattacaga agaaagaaca
     5641 atgctatcga cattccgaaa tgttggagct actttagcaa gtttggtcat tggtgtatta
     5701 acaccattat ttatttatac aagagatgat tcaggcgctc aaatagttcg tggtggatca
     5761 acttttacaa ttgttgcggg aatattttcg atatgtgcac ttatttgtta tttaatatgt
     5821 tataaactaa ctactgaacg agtaagaatt gaccaatctc aatacaaagt ttcattgtta
     5881 aaatctttaa aagggttagt taaaaatagg gcattacttt ctataattgg tgcggctatt
     5941 ttcttactcc ttgctcaatt attaattatg agtatgaata attatgtatt tcctgattac
     6001 tataatgatg cacagggaat agttttaatg aattttatca atcctatttt ggttttaata
     6061 gttgtttcgc ctttaacttt gtttttaagt aaaaaatatg gaaaaaaaga actggcatca
     6121 gtaggaatgt tcttttcagc aatagtatat ttgatcttat ttttgataaa agctagtaat
     6181 atgtatgtat tcttaattct tagttcggtt ggctatatgg gtttgggtgt gtttaatact
     6241 gttatttggg caaatattac tgatgttatt gatgatcaag aagtaaagtc tgatcaaaga
     6301 gaagatggga ccgtatatgc ggtatattct tttgctagaa aaataggtca agctctagct
     6361 ggaggcgtcg gaggttggac tctttctatt ataggctatg actctttagc aaatgtacaa
     6421 acgggagatg ttttagaaaa gttatataac gcttcaacat taattccatc aatttgtttt
     6481 ctgattgtag gattaatttt at
//
LOCUS       DAHHZJ010000148         6397 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.150, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000148 DAHHZJ010000000
VERSION     DAHHZJ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.150"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(<1..478)
                     /locus_tag="NBL02_002336"
     CDS             complement(<1..478)
                     /locus_tag="NBL02_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCD2573009.1"
                     /translation="MKAIIVGAGGFGKEIAFLLQSVSRYELVGFVDDSLKMQNQELLE
                     KPILGTIDLLIELEEETAIFLGIASPDIKEKIYQKINKNNKLIFPNLVAPSALVGINV
                     QLGIGNILMPYTTYTADVVLGNFNMINIGSTIGHDTQIGNYNSVFPSVNISGHVILG"
     gene            complement(489..2306)
                     /locus_tag="NBL02_002337"
     CDS             complement(489..2306)
                     /locus_tag="NBL02_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCD2573010.1"
                     /translation="MFVLTRRRKIATLVFVDSLLLIAANIAAAKFMKPFVAIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAVFGSLSASAVSSIIILLFINRRYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYVETKNMRYLSTDSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVVGFVDDDPNKRNTYLSGKKVLGALKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESARVKVNTMPSIEELASGKISVSKLKTIDVVDLLGRDEVKLDIESI
                     KDQITDKVILVTGAGGSIGSEICRQIIQFNPAKLLLLGHGENSIYLIDRELRTHHQNC
                     PTEIVPIIADIQDREKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAARVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPITVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSIQKVMDFVKSLPQDDEQLAKDIVTFANASNK"
     gene            complement(2319..3083)
                     /locus_tag="NBL02_002338"
     CDS             complement(2319..3083)
                     /locus_tag="NBL02_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HCD2573011.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPIYEEVKKKKFGLF"
     gene            complement(3111..3809)
                     /locus_tag="NBL02_002339"
     CDS             complement(3111..3809)
                     /locus_tag="NBL02_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HCD2573012.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQVLAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(3821..4600)
                     /locus_tag="NBL02_002340"
     CDS             complement(3821..4600)
                     /locus_tag="NBL02_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HCD2573013.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(4616..5560)
                     /locus_tag="NBL02_002341"
     CDS             complement(4616..5560)
                     /locus_tag="NBL02_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCD2573014.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(5606..6385)
                     /locus_tag="NBL02_002342"
     CDS             complement(5606..6385)
                     /locus_tag="NBL02_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573015.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
BASE COUNT         1666 a         1355 c         1162 g         2214 t
ORIGIN      
        1 ctcctaatat aacatgtcct gaaatattta cacttggaaa aactgaatta taattaccga
       61 tttgagtatc atgtccgata gtagagccga tattgatcat attgaaattt cctaaaacta
      121 cgtcagcagt gtaagtcgta tagggcatca aaatattgcc aattcctaac tgaacattta
      181 tgcccactag tgcacttggt gcaactaaat tagggaaaat caatttatta tttttattta
      241 tcttctgata tattttctct ttgatatctg gcgaagcaat tcccagaaaa atagcagttt
      301 cttcttctaa ttctattaac aagtctattg ttcctaatat aggtttttcc aataattctt
      361 gattttgcat ttttaacgaa tcatcgacaa aaccaaccaa ttcatatcta gagacacttt
      421 gtaatagaaa agcaatctct ttgccaaatc caccagctcc tacaataatt gctttcactt
      481 tactccccct acttatttga agcatttgca aaagtgacaa tgtctttagc cagttgttca
      541 tcgtcttgcg gcaggctctt gacaaaatcc atcacttttt gtattgagta acctttgatg
      601 tttcctacaa agattttctc ataaactgct tcatcatttc tttccttatc caacaatagt
      661 tcttcatata gtttttcccc tggacggata cctgtttcaa tgatttcgat ttcgtcttcg
      721 ctatacccgc tcaatcggat catgtttttg gctaggtcta caatcttcac tggttcactc
      781 atatctagaa taaaaatttc gccacctttt gctaatgcgc cagattgaat aactaaacga
      841 ctcgcttcag gaatcgtcat gaagtatcgg gtcatacgaa agtctgttac agtgattgga
      901 ccaccttgag caatttgttc tctgaatact ggaatcacac taccgcgaga accaaggaca
      961 ttcccaaatc tgacagctga gaatttcgta cagccttctt cattcaatcc agtcacgatc
     1021 atttcagcga ttcgttttgt tgaacccatg acgtttggag gattgttagc tttatctgtc
     1081 gataccatga cgaagttttt cactcttgct gctttggctg cttctgctac attttttgta
     1141 ccgaaaatat tgtttttgac tgcttctttt ggattatatt ccatcagtgg tacatgttta
     1201 tgagcagctg catggtagac gatatctgga tgatattgtt ccatgatctc attgattttc
     1261 tcgcgatctt ggatatctgc gatgatcggt acgatttctg taggacaatt ttgatgatgc
     1321 gtgcgtaact cacggtcaat caagtagatc gagttctccc catgtcctaa aagcaatagc
     1381 tttgcaggat tgaattgaat gatttgccga cagatttctg aaccgatcga tcctcctgcg
     1441 ccggtgacaa gaatgacttt gtctgtgatc tgatctttga tggattcaat atccagcttt
     1501 acctcatctc ttcccagtaa atccactacg tcaattgttt ttaattttga tacgctaatt
     1561 tttcctgaag caagttcttc tatagaaggc atcgtgttga ctttcacacg agcggattcg
     1621 actaactcga agattcttcg taattttttt cttgataagg aagggatagc gatcgttacc
     1681 atttgaatgt catatttttc aatcaattcc ggaatatctt ttaatgcacc taatactttt
     1741 tttcctgaaa gatacgtatt tcttttattt ggatcatcat ctacaaaccc gacaacgtgg
     1801 atatcttgag ccgttttgga tccaagaaag ctgttgtata aaatacgccc gccttcgcca
     1861 gcaccgacaa tcaatgtatt tttagcagaa tctgtggata gatatctcat attttttgtt
     1921 tcgacataga tacgccagat caatcggcta ccaataatca ataatagcga aagcaggtaa
     1981 gcaaaaatga ctaaacgtaa actgtatcgc cgatttatga acagcaatat gatgatactt
     2041 gagacagcac ttgcagatag tgagccaaaa accgcaatga tttctcttaa atttgtatag
     2101 cgattgatcc gagtgaatac tttgaataaa ctaccataga ataaataaaa accaatcgac
     2161 aaaccgatag agattaaaat aaggtccatt ggtatagcta caaacggctt catgaattta
     2221 gctgctgcaa tattcgcagc aatcaacaac aagctgtcca caaataccaa tgtggcaatt
     2281 tttcttcttc ttgttaatac aaacacccta cattccccct aaaataaacc aaacttcttc
     2341 ttttttacct cttcatagat tggataactg atttcatccc cgttgatcag atcctttgcg
     2401 acttgtttca tctgtactac tttttcctta ccaaagtcct tggcaatctg ttcatagcac
     2461 tcttttaagt aaaaggtgcg cttagagacc ccatgggcat cagaagcagc catctgcaca
     2521 agattatgtt tgaccatcgt tttggctgtt ttctggatgt ctttgccaaa taccccaaca
     2581 taacttggcg cagtgagttg ggccaaacat cccatatcta ggaacggaag caaatgattt
     2641 gggtcttcgc gaaaatgcgc gttacgttct ggatggacga tcactggtgt ctttcctagt
     2701 tctaacaact caaaaaaaag ccgttctgca tataacggca cttccagtgt cggaaattcg
     2761 atcagaagat atgtatcatc taaatctgta aataaaattt catctcggtg gatgtcttcg
     2821 atgagcgttc cggtaatacg tacttcttgt ccttccaata acgtcaaagg caactgtctt
     2881 ttctctaatt cggcttgtaa cgaagcgact agagaaatga cttgtgattt ttcattgctg
     2941 tattttccat tattgtggtg tggtgtgcat aaaatgtgcg tgatgccttg ctgaatcgct
     3001 ttttcagcca ttgcaatact tgcttctaag ttttctgccc cgtcatcgat tcctggtagg
     3061 atatggcaat gtagatcgat catttctctt gtctcctctc ttcttattcc ttaatttccg
     3121 tagtaataac cagcatcttt gctatgttct gctccgttgt acacaacacc taagactcgt
     3181 gcttgtacca tctccagcag ttcttttgct ttagtcaagg attctttacg tgacatattt
     3241 tcacgtacga ccaataaagt gccatccgtt tttgaagaca tgatctgtgc atctgttacg
     3301 gctactactg gtggcatatc gaatatcacc acgtcaaata actgtcttgc ttcggctaga
     3361 acctgatcca ttcttggcga gcttaacaat tctgatgggt tgggtggctt aggtccacta
     3421 ggcaaaattg acaagttctc gactggcgtt cgttggatca catctgctac acttcctgat
     3481 gaactaagtg ctgtacttaa tccactggca ttgttcagct ggaatgtctt atagacgacg
     3541 ggtttacgta aatctgcatc gactagtaga actcgttgac ctgatttagc aaaaacgact
     3601 gcaatatttg ctgcagtggt ggatttccct tctccaggac ctgatgaagt cacgacaatc
     3661 gttttgattt gctgaccagc tgcggaggca aactggatat tcgtccggat cgtacgatat
     3721 tgttcggcga ttggtgaaga tggatttgtt aatgtgatca aactgactgc atgagtttgt
     3781 gctgcttttt gtttctgtgt tcgtgccatt tttcttcctc ctagactctt gagcggcttc
     3841 tgcgtgatgg ggctgtttta tcgtttgtag cttttggtcg tgcaggagct gttccttttt
     3901 tacggatacc agcgttcaat tctttcgctg acatttctgg aactgttcct aagattggga
     3961 atcctaatgt atccgtcaca tatttttcat ctttcactgt ccgatcaagt aattcgaata
     4021 agaatgccaa acctacgcct accatcattc ctaacactaa gccaatcact agattcaatt
     4081 tattatttgg cgaaacaggt gtcgtattgg caactgcacc tgaaataatc gagattttat
     4141 cgacattcat cacgtctttg gcgttttctt ggaaaacttg tgaagttgtg ttagcgatct
     4201 gttgagcagt cactgcattc gtgcttattg cctggatcga gaacatttgt gaattttgtg
     4261 actgattgac ggagatcgca tctttgattt gtccagcagt cattttcaca ttgtcttctg
     4321 tttctaaacg atctttgact tcatttagta ctaaatcact gacgatcata tctttatacg
     4381 tattgatcat ttgcaggttc gtattgacat cattgacatt tgctgtgtct gattgtggca
     4441 gtgtcacgat caattgtgct tgtgaactgt attttggtgt caagatgaaa aacgtcacga
     4501 ttcctgagat ggccaagcca gcaaacattg agatgatgat tgttaccaag tgtttgcgta
     4561 atgtttcaaa aatttctttc aagctgattg tctcttccat tttttctcca ctttcctatt
     4621 tattctctag attcatttga tcttttaatt ctttttttac acgatcaagt tcttgatcac
     4681 tgactttttg ataggagata ttatcttgca tgaagccttc tccttgcagc tgatcttgct
     4741 tgatcgtacc aaatgcctcc cgataatcta aagcaatttt tttcatttca tcaaagctca
     4801 tgtctgtttt catattcgac tctagagcat tcaaaacgga ttgatagttg ctgacaccgt
     4861 ttagactcaa tacttttttt gctacgcctt ctacgatctt tctttgacgc tcttgtctac
     4921 catagtcacc gtttggatct tcataacgca tacgagaata agataatgcc tgatccccgt
     4981 tcaaagtaat tttaccgatt gtaaagtcat agccatcttg tgagaaagtg aggtcattat
     5041 tgacttcgat accaccaact gcatcaacaa gttcttttag ccctttcatg ttgatagaga
     5101 catagtgatc gattgggatg tctacatatt tttgtacggt atccatcgcc atggcagctc
     5161 cgccgaatgc gtaagcgtgg ttgatcttgt cttttgtgtc acgacctacg atgtcgacat
     5221 acgtatctcg aggaatgctg acgatcgttg tctgtttgtc ttcaggactg actgtggcaa
     5281 ccatcatcgt atctgatctt ccttgatcga cccgacctaa atctccagta tcaatcccca
     5341 tcaatagaac agagaaagat tcttgtttgt ccagatcaac tggtttcgtt cgttctcctt
     5401 gggttcgttc cactgattcg taagttttct caatagaatg tgagagatcc agatataatt
     5461 ttgctcctac tcctacaatt gctaatacta atacgataag aactgctaaa ataatcagaa
     5521 atattttttt ccctcttgat tttcttcttc gtcttgccat agtttcctcc gtttgctaac
     5581 cttgcttgaa tcaacgtttt aactgtcaat agcttatatt gctatagccc atttgctgag
     5641 caacagtttg tgcttttgtt gcgtatgctg cgcgtatttt ttcattccgg atctgctcta
     5701 tttgactgag caccgtcaaa tattgatcgt aagtagcggt ggcagtcact tgaccatcct
     5761 tcaacatcgt atcgaataac tgatctagtt gcgtgacttg cgcgatctga gtatcagcga
     5821 atcctagata ttgcataaca accgttttcc aaggactatc tttaaaaaag ctcatatttt
     5881 cacgtacacg agcgacatct gcacttgtca ccttttcttt gatgatgaca tcgtctacag
     5941 cttgctgcca gtttgataca tttttagtaa ataaatcgtt gacagctgtc tggatcttca
     6001 gttttgattc tgcactatct aactgtgtca cgattgcttt cttctcatct tggattgcaa
     6061 cagcttcttt aggcaattct gattctttga tgtcgtaatc atctgctgaa agtttgacag
     6121 aggatagctt atttgacaaa cttgtgattg tttcttctgt tatttcaggg tttagaaaaa
     6181 ttttttcttt tgtactatac agcttgtcta cttcttttga taaagaagtc aaggtgtctt
     6241 tttgattggt gatggcagat tctgcataat tgatccgttg ctgatgttgt cgctgattat
     6301 tgatatggta accaaacaaa cagatcgcaa tagcacctgc acctaccgcc acagcagtca
     6361 atgacttgac gaatggcttt tccatagatt cactcct
//
LOCUS       DAHHZJ010000149         6358 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.151, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000149 DAHHZJ010000000
VERSION     DAHHZJ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.151"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(1040..1705)
                     /locus_tag="NBL02_002343"
     CDS             complement(1040..1705)
                     /locus_tag="NBL02_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD2573016.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            1885..3867
                     /locus_tag="NBL02_002344"
     CDS             1885..3867
                     /locus_tag="NBL02_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HCD2573017.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            3871..4509
                     /locus_tag="NBL02_002345"
     CDS             3871..4509
                     /locus_tag="NBL02_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2573018.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4537..4965
                     /locus_tag="NBL02_002346"
     CDS             4537..4965
                     /locus_tag="NBL02_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573019.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5422..6333
                     /locus_tag="NBL02_002347"
     CDS             5422..6333
                     /locus_tag="NBL02_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCD2573020.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT         2196 a          968 c         1178 g         2016 t
ORIGIN      
        1 catcaaggtt cctagccttt ttataataaa atgaagcgaa aataatttct tttaaagagc
       61 cgagattatc agctatataa gagtgaattt gatgcaaaaa acagagccga aacagtcttg
      121 gtgttcgtag ctctgcttat atgtttcgaa tttaagacaa agagttggca aaagatgttg
      181 ataaggaaga taagataact gtgttatata aactaggtaa ctaactgtaa caagctaatt
      241 ttaagagatt cttattagaa gacgtttagg aatataacta agaaaatgat tatacaggta
      301 attgtcattt caataatgtt ttctaatttt cgcgtctttg ttttaagaaa aaggccagaa
      361 tcagttggaa aaaagttcgt acgatagtga agaggggcaa aatttcctac taatattccc
      421 actaacaaga gataaaacgg ccaggttggt gtgattcctc tataaaaaaa tgcccatgct
      481 accgaaatcc catagattaa caactgaaca atttttacaa tgaaaaaatt gttctgccat
      541 tactcaaagt ctcttcctcc ttgtatcgtt tatttgtttc attttatcat tatgataggc
      601 gcttgaacaa tccattttgt attttattga gtactcttgg ataaactacc agcgattatc
      661 atatttattt atgttactgt aagccatcat ataaaagtaa atgagcagat catagaaata
      721 taaaaaagaa ctaaaattga tctaaattat gtgcagcttg actacgtacc aatctatatt
      781 acttactaga ttggtcgtta atctattcgg atcgaatgag caaaatattt ttgtgaatgt
      841 ccatccttga ttatgattcc aaaaagaatc attgggaggc tgtggcgtaa atattatact
      901 ataagccaga aatacctatc attctgaact tttttattta ggaactttca tctcaaggct
      961 ttttcaatta atttgtaccc ctgttttttg gataagatca tgtgcattga ttttttctat
     1021 ctctacgttc atctcctact taatgcctat ctggctcctg agggcttctt tttaaggcgt
     1081 cgtcatagtc ttggctgagg atttcttggt aatctgaggg aatggcttcc caagtgagca
     1141 ggcgaacgtt catgacgtgt tttcctttgt gtttgattac agcatggttt ttctcttttt
     1201 ctacctctcc agcgtgaaaa gacagccatg taccatggcc aaagatatca ttacataccc
     1261 aacgttcgat ggaattgtcc acgtatccta cagggagtgt ggtatagccg atttgtggtt
     1321 tcaaaaaagg attgaatcga tcgatcgttt catttttgta ttgataattg ataagaaaaa
     1381 gatagaccaa acaaaagacg agcagaaaga aagatagtgt ctttttgagt gagaagggtt
     1441 gttttgatga ataagatttc ttgttaacaa gaggttcatc tggcaatgtt ggttctattg
     1501 attcggtagc tgattcaata gaaggaaaat caatcggcag acctaaagta tgatgtaaaa
     1561 gtgacagcat ttcaaagtct ggcattgtac gagaattttc ccaactagaa atcgttttat
     1621 cggaaacacc aattttttcg cctaattctt tttgcgtaag cttacaattt ctgcggtaag
     1681 ttcgtatttt ttctccggtg ttcatcttct tctcctttcg attctacagt atatagattt
     1741 attctacatg acctatacac ttgatagata cgtttttttg atgaaaaacg gtatgatttt
     1801 atcaaagaat catttaagta agggagagtg gtgataatca aaaaaaagtt cacagtttta
     1861 ctcacaatca tagtgaccgg tatcatgagt ggttttgcct tggttagtat aaataaacag
     1921 gtactctatc ggacaaatga atttttccat caaacagata cttatactcc gattcgattg
     1981 ccacaacatt ttattgggca agaagaatat gagcagctaa tcatggtatt tgaaaatgtc
     2041 tcatcagaaa ctgagctttc ttatatgaaa aaaacagtaa atgaatccaa tgaagtagat
     2101 acatcttgga acttcaagat acagccaaag cttgaaatga cattgtatta tcaaggggat
     2161 gtcaaatctt ctcacgagaa ggtaccctat tctaatacct cctttcgcgt aacgtccatt
     2221 gaagaatcga tcaaaataaa agattttgaa ggtgaatttt tcttgatgac gagagaaaaa
     2281 gaagcctatg aacgggtcat ttcactattc actgatcgat tcaatcaagc atttcacact
     2341 gattatgcca ctaatatttt tgaagacttt gaaaatagct attcgtattc ggttatttca
     2401 aatacagacg tatataatta tcgagtgtat ttaactattg gattgattct tttattcctt
     2461 ttgttatcat cctttttctt attgatgaat aaagaaatca gtttattgtt gaataatgac
     2521 ttttcattac atcaagtatt gtggtgttta ttggggaaaa agaaaatcat tattacattc
     2581 ataattgcag gaatcattgg tggttttatt tttatgtatt ataaggagca atttctttat
     2641 ttgttccaga tgtacgtttt cttgttcagc ggaatatatg gtatctcttt gatttctctt
     2701 ttattagttg aaaaaatcgc ttcgttaaga gaagcgttca actatatcca atacattctt
     2761 tttacatttg gattgttgtt ttctcttctt ttgaccgttg ccaatgtcga attagcaact
     2821 atcgtttttt cttcttcaca gttagctgtt tcccatgaac caccaaaaga aatcgctggt
     2881 gaggactatc atgtatttta tccagtaacc atcgggaaaa accacgtgga ttttatttat
     2941 tccaatcgtt ttgcatcagc agcagatcag gaattatatg aaactttgaa taaaaacgga
     3001 agtatattag tcaatgtatc agattacttg aatgaagaaa atgaacagtt tggcagaggg
     3061 ataaaaataa atctgaatta tcttaagaaa tttcctttga ttgatgtctc tggaaggttg
     3121 atccaaataa ccgaaaaaga aacgcaccct gttatattga tccctgaacg atatagacat
     3181 acagatttaa aaaatgacct ggcgcaaata acagaatatt atcaagaaat cagtgaaaaa
     3241 gaaccgaaat ttctatttat aaaaaacaat caacccattt atacatttat tccttctatc
     3301 ccctggatcg aacattatcc ggttgttgaa atactgacat tgaaaaacag tatgtatttg
     3361 gaaagaaata ttctcagcgg agaaatctat ccaccgttaa agatcaagat aggctctttg
     3421 tcgaaagaaa ggttatttga gctgattgaa gaaagtcagt tacgtgataa cttgcagtta
     3481 tcatttccgt atactcagac tgaagagata gctgtcaaag tgttaagcag atcatttcat
     3541 tacttgatac agatattttt actcactttc ttttctttct tcttgttgag ctatgtcatg
     3601 ttatgcatat attttgtcta caattgtcgg aaaatcgcta tctttcgttc aagtggttat
     3661 tcgctctttg aaacgtataa agatttcttc atgatgaacc tcatcaaatg gggaacgaca
     3721 agtgtaatct tcctttttct tatagaaaga gagcctaaat atctttttaa tatttttttc
     3781 tttagcttca ttgattttat tctttcagtt gtttttattt tgtctacaga aaagaaaagt
     3841 cagttattgc tgatgaatgg aggttaacaa ttggagccgt tgatagtgat aaaaggtgcg
     3901 aaaaaaatca ttcgaaacaa aacgattctt catttggatc gtttcactgc ggatgctggg
     3961 gatcttgtga ctgttgttgg agaaagtgga gcaggtaaaa gtacgttatt gaatatatta
     4021 ggattgaatg atcagttcag ttcaggacaa ttctatttgt tcgatcaaga gacgaaacaa
     4081 ttaactggga gagagaagcg cttgataaaa agaaatacca tatcttttct ttttcaagat
     4141 tttgggttga ttgaatatga aacaatcgat tttaatttag atattggatt gaagttttcg
     4201 aatttaaatg tttcagataa gcgaacagca aagtatgagg cattgaaaaa agtaaatata
     4261 aacaagaagc taagtacgcc tatctacaca ttatctggtg gtgagaagca aagaatcgcc
     4321 tgtgcgagag tcttattgaa gaatagcaaa atcattttag ccgatgaacc gactgggtca
     4381 ttggataaaa gaaataagga taaagtagtt gaaatcttat cgcaacaagc ttcccaaggt
     4441 acgttagtta tcgtagtcac gcatgatcca tatctgacca aaataggaaa caagaagatc
     4501 atgctttaaa aatcaaaaag gaaaggatgt cgttttatga aaaaagcagt tattatttgt
     4561 gcactaggga taggttttgt tggaggaaac gctatgtcat ctgaagtgga agcaaattca
     4621 gttcacggtg aaatctatta caaatcttta aagccaggag agattcattt tgatgctaaa
     4681 ggtaatatta ttaagtcaaa tttaccagta gaccgggaaa tttctagaac agcaattaat
     4741 catagctctg gaagatgggt ttattatagt gatacaactt gtacgagaaa aacaggtcat
     4801 tctaaccact atagtcgtgt ctataatcgt cacggtgcaa aagcgaaagt tggaaactct
     4861 attcgatcag caaatgcaac aaaaggagta tgggcctatg caagtgcgac aggaaataga
     4921 actgacacat tctcatgttg gtacaatcca accggttggt attaagtatc caaaagttta
     4981 tataaaatag aaaaaacata taatgaaaca gcgttaaaag ttatttttgg tagttttaat
     5041 atattcattt ttagttgtat aaaaatatat cataataaaa acaacaaaaa aatgtctgga
     5101 aaatcgaaca taaaagtgtc tgatttgaat atcccagcag cggaagaaga aaaacaaaat
     5161 cgataacatt tgttctaaga attagttacg ttaaatacgg aacgcaagat agacttgaaa
     5221 aacggtaaca aaagaagtag tatattaagg tgtgatgtgc tacttgacat ttattctgct
     5281 gagcgaactt tctggattgt gtagccttat atctattcag gacgtatcga gcagattagt
     5341 aaaggtgttc ttgatgattt tcatcttgaa cacctttgtt tttagcaatt cgcaaaaaac
     5401 gaaaagtagg agaattgata aatgaaaaaa agatttttag cacttgccat cgtactaggc
     5461 actggcttat tgtcaggctg tacgaatgcc ggtgaaaaaa cagccgttag ttataaaggc
     5521 ggtacaatca gcgaacaaga agtaatggat agcttgaaaa aaatgcaagg agcagattcc
     5581 gctgtgcaac agttgatcgt ttatcaagta tttgaagata aatatggaga tgacgtatca
     5641 accaaagaaa tcgattctca atatgatcaa acaaagaaac aattgggtga ttcatttgat
     5701 tcacaattga aatctgcagg ttacacagaa caaacattca aagacagtat caaacaaagt
     5761 ctagcctttc aagaaggtct aaagaaacat atcaaattga ccgatgaaga tttaaaaacg
     5821 gcttgggaat ctttccatcc agaagtggaa gctcaaatta tccaagtagc aagtgaagat
     5881 gatgcaaaag atgtgaagaa agctgctgat aagggagacg atttctctaa attagccaaa
     5941 gacaagtcaa ctgatactac gacaaaagaa gatggcggaa aagtgaaatt tgattccact
     6001 actacaacgg ttccggcaga agtcaaagaa gcagccttta agttgaaaga tggtcaagtt
     6061 tctgatgtga tcacttctac caatgcttca acttatacta cggaatatta tgtggttaaa
     6121 atggtgaaaa accaaaacaa aggcaacgac atggataaat acaaaaaaga attgaaagaa
     6181 attgcaacag acacaaaatt aagtgacagt accttccaga ataaagtaat tggcgaagta
     6241 ttgaaagacg ctaatgtaaa aatcaaagac aaagactttg aaaatgtttt gagtacattc
     6301 acttcagatt ctagtacagc aagtagtaaa taagcggtat aaataaaaaa tatgaact
//
LOCUS       DAHHZJ010000150         6353 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.152, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000150 DAHHZJ010000000
VERSION     DAHHZJ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.152"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            328..675
                     /locus_tag="NBL02_002348"
     CDS             328..675
                     /locus_tag="NBL02_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD2573021.1"
                     /translation="MTTFERVKMLADKRKISIVELEEKLNFSKNSLYAWKKSKPSIDK
                     LNAVADYFHVSTDYLLGRTDDPNAGVAPEERKLTVEEALASVMSSDGKPLTDNDREIL
                     SAMIEAYLEKKDN"
     gene            711..1106
                     /locus_tag="NBL02_002349"
     CDS             711..1106
                     /locus_tag="NBL02_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCD2573022.1"
                     /translation="MIINELGVKVEERENLDADGHYVACMNTIVIKANLSKYRRQRTL
                     LHELGHASKHHDNYFLYNLAFSLHSKMEYEADRFMIEKLLDRYIAKSELEPHNINYMK
                     FIEDNNLSVRFEPLVKELLKARIYCYAAL"
     gene            1160..1471
                     /locus_tag="NBL02_002350"
     CDS             1160..1471
                     /locus_tag="NBL02_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573023.1"
                     /translation="MIYTEFKEWLEKNTTGYETFIVKATNYQIEKNKNRPPKKRWDDK
                     KIDKAVLEMWKQVVTNLYQTIRKEKGVPLINGKEIWLEFIEEQGLIEFFNDSMAELEF
                     E"
     gene            1483..2631
                     /locus_tag="NBL02_002351"
     CDS             1483..2631
                     /locus_tag="NBL02_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCD2573024.1"
                     /translation="MAMIKQYKKKNGEKAWYFKTYLGIDPLTGKKKYTTKRGFRTQKE
                     AKTALSRLELELQKTGMPTSTNTTFKEAAELWLESYKKTVKESSYSRTKIIFNKHIYP
                     KFGNIKLSKINTAYCQKVVNDWSEKGTSKQYPLFINYMNKVFKYAINIGLTSDNPTLN
                     LLIPKPQIKTEKKLKLYTKEQLELFLNEVSQEQNPYFKNRDYTLFRLLAFSGCRIGEI
                     LALTWDNINFKTNEMAIKKTVARSDKYYISETPKTKKSNRIIYLDEKTIKQLKFWKLE
                     QRKYLFQLGFTKANYLFTNDENNFTINQSVAERYNIYRERAGLPYIGLHGFRHTHASM
                     LYEAGADHKEVQERMGHANIKTTMDTYTHITNSKKEETTQKLTNYINF"
     gene            complement(2724..3017)
                     /gene="rpmA"
                     /locus_tag="NBL02_002352"
     CDS             complement(2724..3017)
                     /gene="rpmA"
                     /locus_tag="NBL02_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCD2573025.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(3030..3374)
                     /locus_tag="NBL02_002353"
     CDS             complement(3030..3374)
                     /locus_tag="NBL02_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HCD2573026.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(3401..3709)
                     /gene="rplU"
                     /locus_tag="NBL02_002354"
     CDS             complement(3401..3709)
                     /gene="rplU"
                     /locus_tag="NBL02_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCD2573027.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     misc_feature    complement(3729..3805)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            3991..4632
                     /locus_tag="NBL02_002355"
     CDS             3991..4632
                     /locus_tag="NBL02_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCD2573028.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            complement(4694..6325)
                     /locus_tag="NBL02_002356"
     CDS             complement(4694..6325)
                     /locus_tag="NBL02_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HCD2573029.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
BASE COUNT         2219 a         1205 c         1087 g         1842 t
ORIGIN      
        1 tgcggaagga gtttttttat tttgtttcat gatgtttcct ccttttcttt aaatatgtaa
       61 gctaacaaaa ttagctaatt ttgttgacag tttctacaaa attttgtaga ataagtgcat
      121 agttaaataa gcacagaatt accctataaa ttaacattct aagtttcccg accttgaatt
      181 tgtttacttt attaggtatc tttcttattg cttgttagct tattaaatta gcttacgaac
      241 atatattact ataaagtttt gtagatgtca acgattatct acaaagtttt taagatgtgt
      301 tttaggcatg atcggagaat cattattatg acaacatttg agagagtaaa aatgttagca
      361 gataaacgca aaatatctat tgtcgaattg gaagaaaaac ttaattttag taaaaattca
      421 ctttatgcgt ggaaaaagag taagccatcc attgataaac taaatgcagt tgctgactat
      481 ttccatgttt caacagatta tttgctagga cgcacagatg atcctaacgc gggagttgca
      541 ccagaggaaa gaaaactaac cgtggaagaa gctttagcat ctgttatgag tagcgacgga
      601 aaaccgctca ccgataatga tagggaaata ctatcagcta tgattgaagc atatttagag
      661 aaaaaagata actaaataag taggtgagtc atttggacag tcaaattgaa atgataatta
      721 atgaactcgg cgttaaggta gaagagcgtg aaaaccttga tgccgatggc cattatgttg
      781 cttgtatgaa taccatagta ataaaagcta atttatctaa gtatagaaga caaagaacct
      841 tattacatga attaggacac gcttctaaac atcatgataa ttatttttta tataacttag
      901 cattctctct ccattcaaaa atggaatatg aggctgatcg cttcatgatt gaaaaattat
      961 tagatagata tattgcaaag tctgaattag aaccacacaa tatcaattac atgaaattta
     1021 tagaagataa caatttaagc gttcgcttcg aaccacttgt gaaagaatta ttaaaagctc
     1081 gcatctattg ttatgcagct ctctaaaatt ttttaagcaa aaaaagaaca tatgttcaaa
     1141 aatagaaagg tgaacaaaaa tgatatatac agaattcaaa gaatggttag aaaaaaacac
     1201 aaccggatac gaaacattta tcgtcaaagc tactaattat caaattgaaa aaaacaaaaa
     1261 tagaccccca aaaaaacgct gggatgataa gaaaatagat aaagctgtat tagaaatgtg
     1321 gaaacaagtc gtgactaact tgtatcaaac aattcgtaaa gaaaaaggag ttccattaat
     1381 taacgggaag gaaatatggc ttgaatttat agaggaacaa ggactgatcg aatttttcaa
     1441 tgatagcatg gcagaattag aatttgaata ggggtaatat tgatggcaat gataaaacaa
     1501 tataaaaaga aaaatggcga aaaagcatgg tactttaaaa cttatctcgg tattgatccg
     1561 ctaactggaa agaaaaaata tactactaaa cgaggattta gaacacaaaa agaagcaaaa
     1621 acagcacttt ctaggttaga actagaatta caaaaaacag gaatgcccac aagtacaaat
     1681 actactttca aagaagcagc agaattatgg ctagaaagct acaaaaaaac tgtaaaagaa
     1741 agttcatatt caaggactaa aataatcttt aataaacata tatatcccaa atttggaaat
     1801 attaagcttt ctaaaattaa tacggcatat tgtcaaaagg tagtaaatga ttggagtgaa
     1861 aaaggaactt caaagcagta ccctcttttc ataaactata tgaataaagt ttttaagtat
     1921 gctataaata ttggtttaac atctgataat ccaacattaa atttacttat tccaaagcca
     1981 caaattaaaa cagaaaagaa attaaaatta tatacaaaag aacagttgga attatttcta
     2041 aatgaagtat ctcaagaaca gaatccatat tttaaaaaca gagactatac gctctttaga
     2101 ctattagcat tcagcggatg tagaatcggc gaaatattag cactcacttg ggacaatatt
     2161 aattttaaaa caaatgaaat ggccattaaa aaaactgtag ctcgttcaga taaatattat
     2221 atatctgaaa ctcctaaaac aaaaaaatca aatcgaataa tttatttaga tgaaaaaact
     2281 ataaagcaac taaaattttg gaagctcgaa caaagaaagt acttatttca attaggattt
     2341 actaaagcta attatttgtt taccaatgac gaaaataatt tcacaattaa tcagtcagtg
     2401 gcagaaagat acaatatata tcgtgagcgt gccggcttac cttatatcgg tctgcatggt
     2461 tttagacata cacatgcatc aatgctatat gaggcaggcg cagatcacaa agaagtccaa
     2521 gaaagaatgg gccacgcaaa tataaaaact actatggaca catatacaca cattactaac
     2581 agcaaaaaag aagaaacaac acaaaaacta acaaattata ttaacttcta agaaagtatg
     2641 gtcaaaagta tggtcaaaaa aatcccttac ttagcaaaaa aagctccatc ccttgccaca
     2701 caagagatag agcagtttat ttattaagct tcttgagcta ctggataaac agacacttgt
     2761 tttttgttac ggcctttacg ttcgaaacgt actacgccat caacttttgc aaataaagtg
     2821 tcgtctccac cgatacccac gtttacacct ggatagattt ttgtaccgcg ttgacggtaa
     2881 agaattgatc cacctgtaac tgtttgtcca tcagcacgtt tagcacctag acgttttgat
     2941 tctgagtcac ggccgttaga tgtagaaccg ccaccttttt tgtgggcaaa taattgtaga
     3001 ttcatagata acaacatggt ctgcacctcc tatttatcat taattgtttt aatttggata
     3061 tattctgagt tttcagcttc tatcgattgt aatcctaata aaagattttc taatagaatc
     3121 tcagcaatat tgttttgttc ttgtgtcatt cctgaagtga cttcggtata aagataaccg
     3181 ccttcttctt cattcatttc aacaatcggc tcaaaaccgg ctaatgaggc aatcccgttg
     3241 acagtgctga tcgctaacgc agaaacaccc gcacaaacga tatcacttcc ataaggacca
     3301 gcatcagcat gcccggtcaa agtgaatgaa acgatctgac ctgcgtcatt tcgcttaaat
     3361 gttcctttga tcatatatag gccttctttc tcaaacgcta ttaagcgttg atcgcgtcga
     3421 taaccacttt tgtatatggt tgacgatgac cttgtttacg gtgtgaatgt tttttaggtt
     3481 tgtatttgaa agtaacgact ttcttttgtt tgccgtgttt ttcaacagta ccttcaacag
     3541 ttgcacctgc aacagttgga gcgcctactt ttgtagattc tccgcctact aagataactt
     3601 cgtcaaaaac aactttttct cctgcttcaa cgtcaagttt ttcaacatag attgcttgtc
     3661 ccacttcaac ttttacttgt ttaccaccag ttttaataat tgcgtacatg ctataaagca
     3721 cctccttctc atatacttag actcgccatc cgaagtgaca gcatatgctg tgcttattac
     3781 ttgttctgag cggttgtagc tgtggagtcc acaattacaa cagtactagc ttaccagaac
     3841 agcaaccgtt agtcaactaa atttaaaatc aaaaagtaaa acaaaaattc aagcaatact
     3901 tctgcttttc tatcctcaca gttctaccaa ataaaatagt cgcattgtgt ttcttttatt
     3961 cttataataa aagaaaaagg aggagcataa atggaatacc caaaagaact gatacaagaa
     4021 gcttctttaa gaatgaacgg ccgtcctgta gaaggattta tcgctgggca aggacctctt
     4081 catcctaaac tgatgctcgt gggagaagcc ccagggaaaa cagagattga tacgcatgtt
     4141 ccttttagcg gtcaagctgg aaaagaattg atgcaggcac tttcttcaac tggcttaaca
     4201 cgagaagagg tgtatatcac cagtgctgta aggagccgcc cgtatcgcgt gactcatcgg
     4261 atcaataaac ggacacaaca gtcagaaact gtttatccaa atcggacacc tactcgttcc
     4321 gaagtatttg cgcatgcacc aatattggat tacgaactga tgaaggtcca gccaaaatta
     4381 attgcgacac ttggaaacat tggtcttcaa cgtttacttg gcaaagaata tacggttacc
     4441 aaaaatcatg gtcaactcta tacagggcct gtcttacaat taacagaaca aaaggatgcc
     4501 tacgaatggt cagaaaaaaa ctatcgaatt cttccacttt ttcatccagc agctattttc
     4561 tacaatcgct cccttgtacc tgttatccat caagactggc aagtactagc ttcactttta
     4621 cagcaatatt aaaaagaaca gacaagacca taaaatcttc agtttacagt cttgtctgtt
     4681 ctttttcatt gttttaatgg ctaattgcag tatatttcgc atgtaaagct gtcaacgcat
     4741 gagcaactgt ttgatgttga tgcgtgaatg tatgttccca caagtgagaa agttgttttt
     4801 ctacttctgg ctgcaaggaa gtatagataa tcgtttgtcc tctttgctct gctttttctt
     4861 gaaattcatc aaaaactgca cgtgccgtca aatcaatact tggtacgcct cgcatggaga
     4921 aaatgacacc atcgtgatga tttagttctt ccaatttatt tttgagtttt tctgaagaca
     4981 taaaaaacag tggaccacta atatacacta ctgtccagtt attcagcttt tccgtttctg
     5041 gcagccccat tctttgccaa tcgatcgttt ctaatgaaat cgtgatagca gcgcttttga
     5101 caacaaaaaa tacacagccg ccgatcaccc cgatcacgat agcaatactt aaatcaaaga
     5161 taactgtaca aatcatcgta aggataaaca atagaactgc tgaccaatat ttattagtaa
     5221 atagctcctt gatcgtttcc cattcattca tacgccaagc cgtcacaatc aaaacacctg
     5281 caagtgctgg cattggaatc tgtgacatca ctggcgcaaa aacaagcatg gagagcagta
     5341 agacaactgc atgtatcata ccagctaatc tagtttgtgc accagatttg atggccacgc
     5401 ttgtacgggc gatggccgca gtagcaggaa tcccgccaaa gaaaggaagc aacagatttc
     5461 caattccttg cgccattaat tcttgattgc tatctaaggg cttatttgcc attcggccag
     5521 ccgaagcacc acatagcaag ctttcaatca ttcctaataa agcaatgcta atagctggaa
     5581 ctgccacttg ttgcaaggca gaaaagctaa aatctcccat attcaaacga ttactactga
     5641 tcaatgtttg tggaatctta cccacagttg ctactggtaa atggaacaat agcataactg
     5701 ctgttgccaa aatgatagcc agcagcgaac taggcattcg tttcgcccat ttttttggat
     5761 agataaacat ccctaaaatg actagccctc ccatgatcaa agaggctgtg tttatcggaa
     5821 atcctatcgt ttgataacta cttagttttt caataacgtt tgtaccttcc gagtgaacac
     5881 caaataaatt atcgatctga cctaatgcaa taatgatagc aatacctgaa gtaaaccccg
     5941 taattacggg tgctgggata aaagaagtca gtgttcctaa tcggaaaagt cctgctgcaa
     6001 gtaaaaatag acccgctaaa aaagtagcaa gcaatacacc ttgcattccc tttgttgccg
     6061 caatagacat caagatagct gccatcgcac ctgtcggacc tgatatttga taaaaaccgc
     6121 ctgataaacc gccaataacg ataccggcaa tgatagccgt aatcaaacct gcggccgcat
     6181 cagctccgct tgatacccca aaagccaagg ctaaaggcaa tgctacagca gctactgtga
     6241 tcccagccaa aagatctttc tgaaatcttg aaagatcata acctgaaaat tcttttttta
     6301 gtgaggaaac ataatttttg aacacctatc taactccttt ttcatgaaat ttt
//
LOCUS       DAHHZJ010000151         6303 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.153, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000151 DAHHZJ010000000
VERSION     DAHHZJ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.153"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            334..714
                     /locus_tag="NBL02_002357"
     CDS             334..714
                     /locus_tag="NBL02_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCD2573030.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            696..1610
                     /locus_tag="NBL02_002358"
     CDS             696..1610
                     /locus_tag="NBL02_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HCD2573031.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            1855..2544
                     /locus_tag="NBL02_002359"
     CDS             1855..2544
                     /locus_tag="NBL02_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573032.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(2665..2751)
                     /locus_tag="NBL02_002360"
     CDS             complement(2665..2751)
                     /locus_tag="NBL02_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCD2573033.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            3427..4377
                     /locus_tag="NBL02_002361"
     CDS             3427..4377
                     /locus_tag="NBL02_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HCD2573034.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            4398..4931
                     /locus_tag="NBL02_002362"
     CDS             4398..4931
                     /locus_tag="NBL02_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HCD2573035.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            4947..5117
                     /locus_tag="NBL02_002363"
     CDS             4947..5117
                     /locus_tag="NBL02_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573036.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            5567..6100
                     /locus_tag="NBL02_002364"
     CDS             5567..6100
                     /locus_tag="NBL02_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573037.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
BASE COUNT         2323 a         1102 c         1174 g         1704 t
ORIGIN      
        1 aaaaaaaaat aaatcgatac attttagaag aatagggttg tttgaatatt acaaagcaac
       61 aatttcacct tcggtgaaat aagcgaaact ttacaaaaag tggctagcaa atgctagcct
      121 ttttttgtat tgtggagcgt ggattttgag ggggattcaa gggggagctt gcgctccttt
      181 gacaagcggt tatttgcacc aaatacggag gtatttggct agcaaatata gagcttgcca
      241 aaccctttta gtgagcttta ctcattgtac ctttttaggt acaatggtat tgcgaacgat
      301 acgtccgtag gacgtactga aagggtggga cttatgagcg aacaacatca aaatttggct
      361 agcgatcatt ctaaaaaata tacctatcga tccgagccaa aacaaatcag ttttcgagtg
      421 agcgaatccg aatttgcaaa gctgaagcag tcagctgaag ctttgcaaat gagtgtgccg
      481 gctttcgtga aagccaaggc acaaaacgcg cgtctggtga caccgaaagt ggcgccagac
      541 attgcccagg caatcgcgcg cgatttagcc aaagctggag ggaatatcaa tcaaattgcc
      601 aagtggtgca atacacacca acacgacgtt gctcctgatg atgctcaacg tttatccgaa
      661 aacctaaaaa tcatgcaaaa ggagctacaa aagatatggc aacagttaaa gtaagtcgaa
      721 ccacttcatg tagccgagca atcaattacg cagaaccacg agcaaccgtc aaaacaggaa
      781 taaactgtga cattgattat gccaaaagtg aaatgaaaca aatacgcacg ctttatggaa
      841 aagacgatcg tgtacaagca cacttattga ttcagtcctt tcgaccagga gaaattaccg
      901 ctgaaaaagc caatcaacta ggggcagagt atgcgaaaaa aatagcccct aaccaccaaa
      961 tcgcgattta cacacataca gacaaagacc atattcataa tcacattgtc attaactccg
     1021 ttaatcttga aactgggaaa aaatttcaag cccatgggca agcattttta aacaaatgtt
     1081 atgacctaaa tgacgagatt tgtcttgaac atgacttaac aatcacggaa cgaggaaaga
     1141 aaaccgaaaa acgaaccatg tctgaaatta aattaaaaga aaaaaatgaa ccggtttgga
     1201 aagacgaaat tcggtttgcg attgatcaaa caatgaaaaa caaaaaaact cgaacgtata
     1261 cccaattttg tgattcactt aaaacattcg gcattcattg cttgaatcgc ggaaaaaatt
     1321 ttacttacga attggtccac aaaaagaaaa aagtacgatc caacaaacta ggaaaggatt
     1381 acgaaaagga gacgatttta catgagctgg acagacgaga acgaacctac aatcaacaac
     1441 gaaccaacga gctacgaaga gctcgagcta gcattcgcga aactgcagaa gaagtacgaa
     1501 gagaacaacg aacttttacg cctgaacaat cggaaattag accgactcga agaaaccatg
     1561 aacaacacgt tgaacgagga acagaccacc aacaagaatt atcgctctaa aacgttaaaa
     1621 gacatcgaaa acgaaataaa gagacaacgt gcctttcttg cgccttatac ccaagaaagg
     1681 atacaaacta cgatggattc acaagtcaaa aaactccacc aagcgctatt agaccagcga
     1741 aaccaagaac aaaaaaaggc tttaattcaa cgatcgatgg atattcttgc ccccttatgt
     1801 tgcttagtac taacattatt agccttttgg ttggtatggg gagaataaac agacatggca
     1861 ttctatttac cctatctact catttctgcc agtattttag gaattatttg gcttagctat
     1921 aaggcttatc aaacaagata ttcattaaaa ggctctaaaa ttcgatttaa gcgattttta
     1981 ttggtcgggg ttatatttgc cttatcaatc gttttaagtg gtttattcgg cgttttagag
     2041 ggtaataaac gtgtttcaag aagcatttta ttaggggata tcactcaaaa gtatgaagca
     2101 gcatgtgata aaaagaaaaa agagcaagca ttagcacaaa aaatggagga atttaccgct
     2161 tgttatgagg acatgaacaa tatttttgtg gatcaagaga aacgtttaac agataaaaac
     2221 atgaaaaaac tcactcgttt ttatcaaaac ttaccagaaa aacagcaaaa agaggtacaa
     2281 gaaaaatatg agcaagtaaa aaaagacgtg caatatttta aagatacgca gacagaagaa
     2341 gcttgttgta acttatttag cgatacaatc cctttttcaa ccagtgaaca agaacgaaaa
     2401 gaacggcaac aatcggtcac atacgaacgt tatgaaacac tgcttcagca agcaacaaac
     2461 atacaaaatc caacgaaaaa agaaacggca ttaaattatc tagaaagtgt caaggaatgg
     2521 ttggatcagc aacaacaaaa ttgaatttga ttagcaatca tgttaaactg aacctgcaaa
     2581 tgatcaaaca ttagatacac gaaagcccca gagaggtcgg attctctggg gctttttttc
     2641 aggttgatgt cgcctttatt gattttactt cttgtttcgg ttattcaacc aataactgaa
     2701 taaagagaca caaacttcta caagaagggg gcaaatgaac cgttcaaaca ttagatacac
     2761 ctccttccag tcgcaagact gtcggatagg cgacaagtta ttatacctta caaaataaaa
     2821 tctgtgtggt gtttttaagt atcccttatt ttcgggttgt tagggcttgc cctgaccgcc
     2881 tgtaaggcgc tttaagaaca agataaggtt ttttacccaa gactgtctta aaacgactta
     2941 gacgagctat cagaggcttc ccccacctcc gttactgact tgtttttagc aaaggagcct
     3001 ttaggcgact gcgcagtaca atgtgagagc atagcgatca cactttttta gcgaccgtag
     3061 ggagcaaggt tttgaattgg cagttttgaa aaatgcgcga acctgttggt agaaaaaaga
     3121 agtggttttt aggcgggaaa ttttgtcacc ctcttttata atctcttttt aaaccttttt
     3181 ttaaacctct ttaccgtcgg ctgtgtcctt actccttcaa tggatacagc gttttataaa
     3241 ctacattttg tctgctttta aactacattt tgtctgcttt taaactacat tttgtctgct
     3301 tttaaactac attttgtctg cttttaaact ctttttttgc actcacgtat gttataatat
     3361 gcattatcac agacaaaacg ttgtttataa cgtctgtatc cattgaagga gtaaggagga
     3421 atatctttgg caaatgaaat cgtgaaatac catcatgaat taaataccat tccgttgcgg
     3481 aaatttacac cagttgaaat gaatctattt ttctctattg tgtctagaat gcgcgaaaaa
     3541 ggcgataaaa cagtccgtct tacttttgac caactaaaag atttaagtaa ttataaagca
     3601 actgctaata atcgtttcat taatgattta gaaatgactt atgataaatt aatggattta
     3661 cgttttggta aaagaagtgc taatgggctc gatatagata gattcgttat gtttagtgag
     3721 tttaaaatac gtggaactgc taaagatcca tatgtagaca tacaagttta cgaaaaagct
     3781 ttacctttac taaacaattt agatgagtgg gttcgctatt cactccaaca atttaacgaa
     3841 ttacaaagta gctatgcaaa gactatgttt cgattactaa aacaatatcg aactaaaggt
     3901 tttgcttatt tttctaaaga ggattttcat gaactattag atattccaaa aagttactgg
     3961 aacaaccaag caaacgttga taatcgtgtt ataaagccta tacgacagga attaaccgca
     4021 atcttcaaag gattatcaat caaaaagaaa tacggaaaag ggcgaggaaa acctgtcgtt
     4081 ggctatcaat ttacgtttaa accagaaatc aaaaaagcag atgattttaa taaacatgca
     4141 ccaaccaaag aagaacaaaa taaagcattg gctttagctg ataaacaaaa agaatacgaa
     4201 gaacctgcta caacaattaa cgaaaaaatc aaacagaaga aacgagaact tgaactcctc
     4261 caacaaatca aacaactaga aaaagagaaa gaagctcttg aaaaacaaac agagtccgca
     4321 ctttcgaaaa aagagaaaaa aagtttatta tctgatttag ataatttatt ccagtagcaa
     4381 cctaggaggc aaccagtatg caaccacaag aaacctttac aataaaggaa ttgtcagaac
     4441 tttttaagat gagtaggcaa gcaatatcga aacatatcca aaaattagat tcttccatga
     4501 tagctaaaaa tgagcgtggt tataaggttg tattgcgttc tggagtattg caacttgctc
     4561 gcaacttagg caaccaaatg ttgctagata ggttgcacga gtacgaacaa gacaacgata
     4621 aaaatgttaa tataccaagc aaaaacgtgg ttacaaacaa gttgctagat cagttgcgtg
     4681 aagaaaatga atttttacga gaacaacttg ccctcagaga gcaaaataat tctcatatac
     4741 aaaaactact cgaccagcaa caacaactca ccctacaagc caacaaacaa atcgaaaaac
     4801 tgcaagaaaa actacagttg acttatacag aagaagctcc agagaatgac tcaacgactc
     4861 tttctgaagc aacagaaaca atagacaaac aaattgatac tgaaaaaaaa tggtggcaac
     4921 tttggaaata actggaggga acattcatga caagaaaaaa tcttctattc ggttctattt
     4981 tactaggaat tctaggattc agtccgatgt tattgtcagt gatcaataaa gggaataaac
     5041 atggtgaatt gaaattgaat ccaaattatt acaacagtcc taacgaagaa ttgtttgtaa
     5101 aagaaacaga taaataataa aaaagactgc tctttcgagc agccagttaa aaaataatga
     5161 gtgtttattc ttgaaaaagc aacgcgctta tgaagcgcta ccaaaaattt atcatatttt
     5221 attaaaaata acaacccagt aaaaaatgac tggtaaaaat tcacaaagaa acggaaagaa
     5281 gcaaccgcag aaaatcaagc ttaaaaagag cttttgacct agaccaatcg aaaatcacaa
     5341 agtgagcgcc tgtatctatt gctacaggcg ctacttggca tagtgtacat tatgtcaaca
     5401 agaatttaat ataacatatt ttcctaatgc attctaacta taccacatta tcaaaaatat
     5461 aaattatctt tactaaataa aatattgaca agcctatacc ttttgtttta tgattttcac
     5521 aaagaaaacg ttttctatat atttttctgt atcaggagga aattctatga aaaaatttac
     5581 tttaggggta gctttgttaa ctattggggc agttgtttta ccgtcaacta ctatagttta
     5641 tgcttcagaa aattcccaag tggtatcatt aaacgaaaac aatgctactg aaaatgtagt
     5701 tattgagtac actgaagatg gtatggtcgt aactaaatat gaggatggtt atagtgcgga
     5761 agattttgca aattcttcag atatcatgac aagagctgca gttgcaagat ggggcaaaaa
     5821 ggaatataca aatattgctg taaccactgg agtagctgct aatgctatta atgctgcctt
     5881 ttgggcagga gggaaagcta gcacttctat gtttggtatc ccaggttggg caattggtgc
     5941 tttgttaaca ggtgcaagtt ggactaaaaa aggtgcttct ccagggaatg cagttgcaaa
     6001 agaatgggac aaaaatggta atggctggat tggattttat taccaaaggg gatatgatgc
     6061 agctggaaga gttgtagcta ctagatattc aacgttataa gttaaggagt gatgtttttg
     6121 atatctaaat cattaaaatc attaaaaaca tttactaaaa agaatccgca aatagctact
     6181 attcgtattt ttatatttgc tatagctgtt gctataattg ttaatatgct aagcggaggg
     6241 caattataca aatctggttt atttttagtg ttaatcttag ttgtcttaga cataataaag
     6301 tct
//
LOCUS       DAHHZJ010000152         6173 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.154, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000152 DAHHZJ010000000
VERSION     DAHHZJ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.154"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(<1..370)
                     /locus_tag="NBL02_002365"
     CDS             complement(<1..370)
                     /locus_tag="NBL02_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCD2573038.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLN"
     gene            1007..1093
                     /locus_tag="NBL02_002366"
     CDS             1007..1093
                     /locus_tag="NBL02_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCD2573039.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(1214..1903)
                     /locus_tag="NBL02_002367"
     CDS             complement(1214..1903)
                     /locus_tag="NBL02_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573040.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1910..2188)
                     /locus_tag="NBL02_002368"
     CDS             complement(1910..2188)
                     /locus_tag="NBL02_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573041.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(2148..3062)
                     /locus_tag="NBL02_002369"
     CDS             complement(2148..3062)
                     /locus_tag="NBL02_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HCD2573042.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(3044..3424)
                     /locus_tag="NBL02_002370"
     CDS             complement(3044..3424)
                     /locus_tag="NBL02_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCD2573043.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3874..4161)
                     /locus_tag="NBL02_002371"
     CDS             complement(3874..4161)
                     /locus_tag="NBL02_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HCD2573044.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(4181..4405)
                     /locus_tag="NBL02_002372"
     CDS             complement(4181..4405)
                     /locus_tag="NBL02_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HCD2573045.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4860..5030)
                     /locus_tag="NBL02_002373"
     CDS             complement(4860..5030)
                     /locus_tag="NBL02_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573046.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(5045..5578)
                     /locus_tag="NBL02_002374"
     CDS             complement(5045..5578)
                     /locus_tag="NBL02_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HCD2573047.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5599..>6173)
                     /locus_tag="NBL02_002375"
     CDS             complement(5599..>6173)
                     /locus_tag="NBL02_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HCD2573048.1"
                     /translation="LDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFAYFSKEDFL
                     ELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEI
                     KKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQIKQLEK
                     EKKALEKQTESALSEKEKKSLLSDLDNLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 tatttaacaa aggcagtgct ttttcgtaaa ttcgtatatc tacgtaagga ttttctacgt
       61 ccccgttgat tttaaattct gtaaacatta caaacatatc gtaagaaagc ccagactcac
      121 ttctacgacc aaagcgtaaa gataagattt tttcataagt tgcttttaaa tcttctacaa
      181 atcgtacatt tgcagttgct ttgtagttac ttaaatcttt tagttggtca aacgtaaaac
      241 gaactgtctt atcccctttt tcacgcattc tagacacgat ggaaaaaaac aaattcattt
      301 caaccggtgt aaattttctt aaaggaattg tatttaattc atggtggtat tttacaatct
      361 cattagccat ataatcacct caatccaatt atgaatgaca ttttttaaat gtcaagacat
      421 tcatatttga cgttcaaaca gacaaaatgt ccttcataaa cagacaaaat gtccttcata
      481 aacagacaaa atgtccttca taaacagaca aaatgtcctt cataacattc tgtatccatt
      541 ggtagagtaa ggacacagct tacggtaaag aggtttaaaa agaggtttaa aaagagatat
      601 aaaaagaggg gggctaattt tcccgcctaa aaaccacttc ttttttcttt aaacaggttc
      661 gcgcattttt caaaactgcc aatccaaaac cttgcttcct acggtcgcca aaaaggtgtg
      721 atcgctacgc tctcacattg tactgcgcag tcgcctaaag gctcctttgc taaaaacatg
      781 ttggtaaaga ggcctttgag cggttctgaa tgcttatcta agccgtttta agacagtctt
      841 ggataaaaat ccttatctag ctttaaagcg ccttacaggc ggtcagggca agccctaaca
      901 accctaaaat aagcgatact taaaaacacc acacaagttt aattttttaa gatataataa
      961 cttgtcgcct atccgacagt cttgcgactg gaaggaggtg tatctaatgt ttgaattgtt
     1021 catttgcccc cttcttatag aagtttgtgt ctctttattc agttattggt tgaataaccg
     1081 aaacaagaag taaaatcaat aaaggcgaca tcaacctgaa aaaaagcccc agagaatccg
     1141 acctctctgg ggctttcgtg tatctaatgt ttgatcattt gcatgtttag tttaacatga
     1201 ttgccgatca aattcaattt tgctgttgct gatctaacca ttccttgaca ctttttagat
     1261 aatttaatgc cgtttctttt ttcgttggat tttgtatgtt cgttgcttgc tgaagcagtg
     1321 ctttataacg ttcgtaggta actgattgtt gccgttcttt tcgttcctgt tcactagttg
     1381 aaaaagggat tgtatcactg aataaattat aacaagcttc ttctgtctgc gtatctttaa
     1441 aatattgcac gtcttttttg gttcgctcat agttgtcttg aacctctttt tgctgttttt
     1501 ctggcaagtt ttgataaaga cgagtgagtt tttccatgtt tttatccgtc aaacgttttt
     1561 cttgattcac aaaaatatcg ttcatttcct cataacaagt ggtaaactct tccatttttt
     1621 gtgttaacac ttgctccttt ttctttttat cacatgctgc ttcatacttt tgagtgatat
     1681 cccctaataa aatacttctt gaaatacgtt tatttccctc taaaacgccg aataaaccac
     1741 ttaaaacgat tgataatgta aatataaccc ctggcaataa aaacctctta aatcgaattt
     1801 tagagccttt taatgaatat cttgtttgat aagccttata gctaagccaa ataattccta
     1861 aaatactggc aaaaatgagt agatagggta aatagaatgc catttctatt tattctcccc
     1921 ataccagcca aaaggctaac agtgtcatga ttaagcaaca taagggggca agaatatcca
     1981 ttgatcgctg cataattact tttttctgtt ctttatttcg ctggtttaat agcgcatggt
     2041 ggaatttttt gacttgtgaa tcaattgtag tttgtattct ttctttggta taaggtgcaa
     2101 gaaaagcacg ttgtttcttt atttcatttt ctatgtcttg taacgtttta gaacgataat
     2161 tcttgttggt agtctgctcc tctttcaacg tgttggtcat ggtttcttcg agtcggtcta
     2221 atttccggtt gttcagtcgt aagagttcgt tgttctcttc gtacttcttt tgcagtttcg
     2281 cgaatgctag ctcgagctct tcgtagctcg ttggttcgtt gttgattgta ggctcgttct
     2341 cgtctgtcca gctcatgtaa aatcgtctcc ttttcgtaat cctttcctag tttgttggat
     2401 cgtacttttt tctttttgtg gatcaattcg taagtaaaat ttttcccacg attcaagcaa
     2461 tgaatgccga atgttttaag agaatcacaa aattggttat acgttcgagt ttttttgttt
     2521 ttcattgttt gatcaatcgc aaaccgaatt tcgtctttcc aaacaggttc atttttttct
     2581 tttaatttga tttcagacat ggttcgtttt tcgggtttct tccctcgttc agtgattgtt
     2641 aagtcatgtt caagacaaat ctcgtcattt aggtcataac atttgtttaa aaatgcttga
     2701 ccatgggctt gaaatttgtt cccagtttcc agattgacgg agttaatgac aatgtggtta
     2761 tgaatatggt ctttgtctgt atgtgtgtaa atcgcgattt ggtggtcagg ggctattttt
     2821 ttcgcatact ctgcccctag ttgattggct ttttcagcgg taatttctcc tggtcgaaaa
     2881 gactggatca ataagtgtgc ttgtacacga tcgtcttttc catacagcat gcgaatttgt
     2941 ttcatttcgc ttttggcata atcaatgtca cagtttatcc ctgttttgac ggttgctcgt
     3001 ggttcggcgt aattgatcgc tcggctacaa gaagtggttc ggcttacttt aactgttgcc
     3061 atatcttttg tagctccttt tgcatgattt ttaggttttc ggataagcgt tgggcgtcgc
     3121 caggatcaac gtcgtgttgg tgtgtgttgc accacttggc aatttgattg atattcccgc
     3181 cagctttggc taaatcgcgc gcgattgcct gagcaatgtc tggcgccact ttcggtgtca
     3241 ccagacgcgc gttttgtgcc ttggctttca cgaaagccgg cacactcatt tgcaaagctt
     3301 cagctgactg cttcagcttt gcaaattcgg attcgctcac tcgaaaactg atttgttttg
     3361 gctcggatcg ataggtatat tttttagaat gattgctagc caaatattga ttttgttcgc
     3421 tcataagtcc caccctttca gtacgtccta cggacgtatc gttcgcaata ccattgtacc
     3481 taaaaaggta caataagtaa agctcactaa agctcactaa agggcttggc aagctctata
     3541 tttgctagcc aaatacctac gtatttggtg caaataaccg cttgtcaaag gggcgcaagc
     3601 tcccccttga atccccctcc cccccactct ccacaataca aaaaagacta gcaaatgcta
     3661 gccacttttt gtaaagtttc gcttgtttca ccttcggtga aattgttgca aatcaataat
     3721 taggtagagc tacatttttt tcgttgttca aagattctta tgccatttac tattaataaa
     3781 tctataaata tgataatttt tcggctctat aatttatggg actgatgaat cagaattgat
     3841 tcttcagtcc cattttcatt ttattccaaa gaattaactc caaataccaa tagaagccca
     3901 tcctctatat acttgagatt ccttactaac aatttcttgt aattctatta aagatttagg
     3961 taatccaaat ttatgtttta aaatatatct tccaagaaaa ctacttaatt tcatactaac
     4021 ttcaccaaca ctttttccct cactgatttc tttagaataa agaaagagct tttcttttaa
     4081 gtcaggatag tcatctatag ttgcttcatt gtatacttta ctaattgcat ttaaaagttt
     4141 ttcttcttta gtaaaatcca ttataataac ctccctacca ctaccttcta ggtgcccatg
     4201 gaccatgatt aacccaacca ttaacaataa tttttccaat ttctccttta gcttgattcc
     4261 aatctaccca acatttttct ttgttacaat aaagaccatt tccatagtaa gtagctgcat
     4321 caacttttat tcctgtaccg atgccagcta gacaagttcc taatattcct agaataacac
     4381 aatgttttaa tactttcttt ttcatgattg tttttccttt tcgttttttt aaaattgctt
     4441 gttatattaa ataatcttag taattttatt atataacatt ttattatata acaatgatta
     4501 acgtcataaa aagtagtttt gaattcataa tcgtttaaat aaaattataa gatcagttat
     4561 ttaaacagct agtagacata atgtgtatac taccaagtaa cgcctgtacc aagggatcca
     4621 ggcgctgatt ttgtgaattt ttattggtct aggttaaaag ctctttttaa ggcttggttt
     4681 tctgcggttg tttctttcta attctttgtg aatattgatc ggtcattttt taaatacttg
     4741 ttatttttgt ttgtgtttgt tatgcttttt acatatctca tattcattta ttattctttt
     4801 ctaaggttaa taataggata atctctttca gaaagactgc tcgtacgagc agtcttttct
     4861 tatttatcaa gtttttctac aaatacttcc tcattttgac tgttgtagta attaggattg
     4921 agttttagtt cccctttttt atttcctttg ttggccactg ataataacat tgggctaaag
     4981 cttaatattc ctagtaggat agaaccgaat agcaagtgtt tttttgtcat aagtatttcc
     5041 ttcttcattt ccatagctgc caccattttt tccaattctt tgtttgttcg tccattgttt
     5101 ctgttgcttc agaaagagtc gttgaactat tctctgaaga ctcttccgta taagtcaact
     5161 gcagttgctc ctgaagtttt tcgatttgtt ggttggcttg cagggtgagt tgttgttgct
     5221 ggtcgagtag tttttgtata tgagaattat tttgctcttt gagggcaagt tgttctcgta
     5281 aaaattcatt ttcttcacgc aactgatcta gcaacttgtt tgcaaccacg tttttgtttg
     5341 gtatataagc atttttatcg ttgttttgtt catgtttatg caacttatct agcaaccttt
     5401 gattgtctaa gttgcgagca agttgcaata ctccagaacg caatacaacc ttataaccac
     5461 gctcattttt agctatcatg gaagaatcta atttttggat atgtttcgat attgcttgcc
     5521 tactcatctt aaaaagatct gacaattcct ttattgtaaa ggtttcttgt ggttgcatac
     5581 tggttgcctc ctaggttgct actggaataa attatctaaa tcagataata aacttttttt
     5641 ctctttttcc gaaagtgcgg actctgtttg tttttcaaga gcttttttct ctttttctag
     5701 ttgtttgatt tgttggagga gttcaagttc tcgtttcttc tgtttgattt tttcgttaat
     5761 tgttgtagcg ggttcttcgt attctttttg tttatcagct aaagccaatg ctttattttg
     5821 gtcttctttg gttggtgcat gtttgttaaa gtcgtctgct tttttgatct caggtttgaa
     5881 cgtaaattga tagccaataa caggttttcc ccgtcctttt ccgtatttct ttttgattgt
     5941 tagtcctttg aagattgcag ttagttcttg acgtataggt tttataactc gtttatctat
     6001 atcaggttgc ttataacttt taggaatatc taataattca agaaagtctt ctttggagaa
     6061 atatgcgaaa cctttggttc tgtattgctt taacagacga aacatagtct ttgcatagcc
     6121 actttgtagc tcgttgaatt gttgtaaaga atagcgtacc cactcgtcta aat
//
LOCUS       DAHHZJ010000153         6145 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.155, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000153 DAHHZJ010000000
VERSION     DAHHZJ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.155"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            283..522
                     /locus_tag="NBL02_002376"
     CDS             283..522
                     /locus_tag="NBL02_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573049.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(884..1144)
                     /locus_tag="NBL02_002377"
     CDS             complement(884..1144)
                     /locus_tag="NBL02_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573050.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1329..1826)
                     /locus_tag="NBL02_002378"
     CDS             complement(1329..1826)
                     /locus_tag="NBL02_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HCD2573051.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1956..2666)
                     /gene="budA"
                     /locus_tag="NBL02_002379"
     CDS             complement(1956..2666)
                     /gene="budA"
                     /locus_tag="NBL02_002379"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HCD2573052.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2679..4343)
                     /gene="alsS"
                     /locus_tag="NBL02_002380"
     CDS             complement(2679..4343)
                     /gene="alsS"
                     /locus_tag="NBL02_002380"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HCD2573053.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4548..5210)
                     /locus_tag="NBL02_002381"
     CDS             complement(4548..5210)
                     /locus_tag="NBL02_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCD2573054.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(5220..6035)
                     /locus_tag="NBL02_002382"
     CDS             complement(5220..6035)
                     /locus_tag="NBL02_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCD2573055.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
BASE COUNT         1949 a         1276 c          940 g         1980 t
ORIGIN      
        1 ctatcctcct agcaactatt taaataccca aaaaaattac ctctagataa tatcatacct
       61 acgttttcac ttataaattt tttcataaaa aaagcgttta gaggcataat cgttaataca
      121 gatggtaacc aatgtatctg gtttgaactg ggatatcacc ctctccatat atgtattact
      181 aagactaata gatactataa aagacttgat agttttgcat tgattttgcc taatactgag
      241 cataggtgta tgctaaacga tactataagg agtgtgtaca taatgaaaag aattctgttt
      301 atcagtttat caaatttatt gttacttact gcttgtggga cttctgcatc aaagaacata
      361 ccttcagata cgcaaatttc gaatagtatc gattcaacaa tttctacaaa tgagaaaaat
      421 tctccggtag aaacttccga tataaaagtt tctacctctg caaaaaacag ggtaaagaaa
      481 ctgtcagcac gtctccttta tttcaaacaa actctgaatt agcttcttca tcttcttcag
      541 caaataatga tgatagtttc gcgtttactc ctaactaaat gcaaccttta caaatgcttt
      601 acgataacaa gcctgagcta aatgatcctg atatcagttt tgcatttttt gaaatgattg
      661 gatcggatga tctttttaaa actactagtt tgtctatccg aaaataggac agttctggca
      721 gtataggttt ttatcgagtt tctccaccag gcctcgttac agagacagat tctctaggcg
      781 aaccttttga ataaatcaaa aatcatgaac aatcactgcc aactaaataa agtgccttca
      841 aaagcttggt cttctcgctt ttgaaggcac tttatttaaa tctttatgaa actaaagtat
      901 gttcatattt gttgctatag ttatatagat attctgttgt ttccaactca taaacgtgag
      961 tatattttgc cactactttt ccgcattttt ctaatatttc attgcgatcg acgtcatgat
     1021 attcgtctac gcagatcaca acacaatttt ctaatttact gattacttct ccaaccactt
     1081 tttgtttcaa gccaataggt tggcaaatat atttttttcc taaaatgata gtttcgttat
     1141 tcatatttca tccatcctta atgtttttcc attatttaaa atgataatac cattaaaata
     1201 tgattatgaa aatcaataac acttgtgatt ttataaaaaa ataaaaaaac cgaaaaacag
     1261 aaatgcccac tgtctttccg gctcttttta ttcttgattt ctcttctttt ttgtttcaat
     1321 agacgatttt agttattaat aaatgaaaaa gcatttatat taaaatttgc taataaaaga
     1381 tctacatatt tttctagaaa tgcttgatcg atttcatcat accctttcgt tactccgcaa
     1441 tcgatatcta aaacaccgat taaattacct tcttttacca ttggcactac gatttctgat
     1501 tgtgcggctg aatcgcagga aatgtaattt gcatgggttt tgacatcatc aacgatcaat
     1561 gtttcttgtt tttcagctga ttctccacaa actccttttc ccatcttgat acgtgtgcaa
     1621 gaaactcgcc cttggaacgg tcctaaaatc aattcattac catcgaataa ataatatcct
     1681 gcaaagacag tctcaggcaa agtctcattc aacaatgcag atgaatttgc taaatttgct
     1741 aaccagttat cttctgcttc taataatcct tgttgctgta ataataaaat ttcgtatgca
     1801 tcttttttct gtttttctgt ccacatgata aaaaccgctc ctttttgatc tatggagaag
     1861 attatagctc agtttttttg taaacgctag gtttcctcct caaaaaaacg agatacgtca
     1921 ggttaatcac cttgaacgca tctcgctgtc tttttctatt ctgcttgttg gatttctgct
     1981 gctaaatcag cataatcaat cgtttcgtga acaaaagtcg gatcatcaac tggaaaatgc
     2041 tgtacaaggg acgagatgcg gctaatctca acttttcctt cagccacttc aaaatccatt
     2101 acatgtccgc caaatgtatg tgtatcatcg ataaaatgca agtgaaatcc cgctgcagca
     2161 actccttgga ataattcagg cgtaaagaac ccgactaatg ttccttggac attgctttgt
     2221 tggaattctg gttgagactc tgaaattttt gctagtcttc tgtaaggttt ttcttgcttc
     2281 ggcatgatac ggatgtgcat atttttgaat aatccagtta tcttcactgc caaaaatagg
     2341 ttttccccgg cttctcttac taatatgtct tttttcaatt gttctccatc tgcagttttt
     2401 gagacagaaa aactagtatc tggcgtaaaa tctgtaattg ctgcataggg ggtaagttca
     2461 cttccatcta attgggcaac tgagccatct gatcttcctt gataagctac gccatctaaa
     2521 aagatgactt caccatcaag cccatgcaat gttcccaatc ccagagttcc tttttctaat
     2581 atatgagcaa tcgtttctgt tccatccatc aagccagcca tcaaagctcc taatgtcccg
     2641 tgctggtata agattttttc agtcatgtcg acacttcctt aatagaattg atctggtaag
     2701 attgtttctc ccaatttctc attatcacgg taatcaatcg gaatatcaat aattacgggt
     2761 ccttctgtcg caaatccttc ttctaaagct ttttctaatt cctctacact tgtagctcgg
     2821 attcctttag cgccaaaagc ttctgcatat ttcacaaaat caactggacc aaagtctaca
     2881 cctgacgccc gctgatattt cattttttct tggaactcta ccatattgta gtgtccatca
     2941 ttccaaatca aatgaacgat attcaatttt ttacgtaccg ccgtttctaa atcctgggca
     3001 gagaataaaa atcccccatc gccggaaact gatacgattt gtgtatttgg acgcaccaat
     3061 gcagcagaaa tcgcccatgg caatgcaaca ccaagcgttt gcataccatt actgaataac
     3121 aaatgacgag gttcataact tctaaagtgg cgtgccatcc aaatataatg actacctaca
     3181 tctacggtaa cagtcatatc atctgttact tttgactgaa gcgtgttaat aacttctaaa
     3241 ggatgcaaga tacctgcttc tccttttgat tgaacaatgt cccgctccgt taattctgct
     3301 tgaagagatg ctaaatattc ttttgcatct tctgacactt gttgaggctc taatgaacct
     3361 gtcaataaat caagagtggc tgagatatca ccgatcaatt ctctttcagg ctgcataaaa
     3421 ggatcgattt cagctggcgc ttcatcgatc acaatgatcc gcgcatcttt ttccgcattc
     3481 cagtttcttg cttcatattc gattggatca tagccaatag caataactag atcacttcgt
     3541 ttcaaaagca tatctcctgg ttggtttcta aataaaccca cacgaccaaa gaaatgcgct
     3601 tctaattcac gtgagatcac tccagctgct tggaatgtct ctacaacagg caatgctgtc
     3661 tttccaacta attgcctgat ggctaacgtt tctttttcac ttgatcctcg catacctact
     3721 aatagtaccg gtagttttgc ttctctaatg cgttcagcta ggtaacggat atcatctgca
     3781 gaagcagaac ctaatttagg atcgctcaat ggcttgatga cattcccttg gacaggtgca
     3841 tccactacat cttgcgggat actgataaag ctcgcgcctt tttttgaact cttagccata
     3901 cgataagcat tggcaatcac ttctgaaatt gtttctggat cttggatttc tgcactgtat
     3961 tttgtgattg gctggaacag tgctgcatta tccatgcttt gatgggtcaa cttcaataga
     4021 tcgcttcgtt ttacttgtcc ggcaattgct aaaactgggt caccttctgc tgtcgctgtt
     4081 accaaacctg tagctaagtt actagcacca ggaccacttg ttgcgataac tactcctggc
     4141 tctcctgtaa tacgtccgat tgcttgtgcc ataaatgcag cattttgttc gtgacgagtc
     4201 acgattaatt caggaccttg atcctctaat tcattaaata caccatcgat ttttgcacct
     4261 ggtataccga atacataatc aacatgatga ttaatcaaac tttctactac agatttcgaa
     4321 ccttgtttca ttttttcgtc cattgaacat cgtccttttt atgctttatt tttaattatt
     4381 agttagaaat aatcacaaat aattgataca gtttctaaac ctgtttcatt caaaagtata
     4441 gcacgtttta tttcatttgt ttagccatat gtttcaaaaa atgaaaaaga atgacagttt
     4501 ttacataaaa actgtcattc ttaattgaaa gtttcaattt ataaagtcta cagatccata
     4561 gttctcatca atttacgatt gttgaagtaa atcaaaccaa atacgataga aggacctagt
     4621 agaatacacc attggggctt tacgaaaaag aagagaaaca aacttgtcaa tagtaaaaaa
     4681 tctagtaaac tcacatacca ttttttcaac gtatagtaga acgctatccg tagaacttga
     4741 tttggtttcg acgctggatt tctcacctga aaataccagt agttgatgct aaaggaaact
     4801 gataaacaag caagtaaaac aaacaacgga atcaaccatt ttccataaga aaaacgtata
     4861 aaaaagaaac aatcaaaaat cattatcaca ctaactatcc aagcaaatag ccaataaaaa
     4921 aatcccttta gccaaaattg gcgaaacgtc tttatgaaca aacgtactgg tgaaaaactt
     4981 ttttcttttg ctaattcttc tgtcatacct atcatcacga tgcaacctgg accaataaaa
     5041 agtaaagaaa aaataaagga aaagacattc cttccatcta tcgacaaaag gaacgcagct
     5101 gctatataca agatattgac acaccaaaaa caaaggctac ttgccaatga ttgatacaac
     5161 caataaaaga attccatata aacatttttg tcaaaacgct gctctttcat aatcacctcc
     5221 tattccttca ttcctgatga agcgatccct tctacaaaat atttttgcat cgctaaaaat
     5281 ataaaagcaa caggaagaac agaaacaact gaggcagcca tgattaatgc gtactctgca
     5341 tcaaatagcc caataaacat tttcaaccct aactggattg ttttattttc cgtagaagac
     5401 aaataaatga atggccccat ataatcgttc catgtattaa cgaatgtgat gatcgtcaaa
     5461 ctggcaatcg ccggttttgt aagaggtaaa atgatcttgc gatagattcc ccactctgat
     5521 aagccatcga tccgcgcact ctcacataaa gaatctggga tactactgta gtattgcttc
     5581 aacaagaaca caccaaaagc attgaacgct tgtaatagca ccaacgacca caacgtatat
     5641 gttaaaccca cttttctcat cataatgaac tgcgggatca tataagactg ccaagggaca
     5701 gcaatcgtgc cgatatatgc taaaaataaa atatcgcgcc ctttaaaatt tagtttagaa
     5761 aacccgtaag cagcaaaact agaagtaaac aactgaatca acgtgatcac aacgcttaaa
     5821 aaagctgtat tcttaaaaaa tgtcactaaa ggaatacgag accaaataac cagataattc
     5881 tctaaatgcc aagttttagg gatccattga atcggaatcg tgaacacttc attatttgct
     5941 ttgaatgaag cagaaaccat ccataaaaaa ggaatgatcg tcaataaagc taatacaatc
     6001 agcagaatca tacccaacca ttgaataaag ggcatgtttt ttgaacgaat ttttgctttt
     6061 tttctttcat ttcgaacacc tccaaagtta taatcttcat ttctttgtaa tgattacaat
     6121 ccagaacaac ggttattata gctga
//
LOCUS       DAHHZJ010000154         5984 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.156, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000154 DAHHZJ010000000
VERSION     DAHHZJ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.156"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(<1..404)
                     /locus_tag="NBL02_002383"
     CDS             complement(<1..404)
                     /locus_tag="NBL02_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCD2573056.1"
                     /translation="MAKYTFELKLKIVHDYLDGKGGSDYLAKKYSIKAPSQVKRWINA
                     YQEFGEEGLVRKRQKKKYSVQFKLDAIELYLTSELSYREVANTLNMNNPNLIASWMRQ
                     FREGGIDGLSKTKGCPPILSKKNEPKNKKKSTT"
     gene            647..1102
                     /gene="tnpA"
                     /locus_tag="NBL02_002384"
     CDS             647..1102
                     /gene="tnpA"
                     /locus_tag="NBL02_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCD2573057.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1367..1465
                     /locus_tag="NBL02_002385"
                     /pseudo
     CDS             1367..1465
                     /locus_tag="NBL02_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2148..2966)
                     /locus_tag="NBL02_002386"
     CDS             complement(2148..2966)
                     /locus_tag="NBL02_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCD2573058.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3127..3816
                     /locus_tag="NBL02_002387"
     CDS             3127..3816
                     /locus_tag="NBL02_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCD2573059.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            3830..5332
                     /locus_tag="NBL02_002388"
     CDS             3830..5332
                     /locus_tag="NBL02_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCD2573060.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5345..5821
                     /locus_tag="NBL02_002389"
     CDS             5345..5821
                     /locus_tag="NBL02_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCD2573061.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
BASE COUNT         1992 a          909 c         1079 g         2004 t
ORIGIN      
        1 gttgtagatt ttttcttatt tttgggttca tttttcttag acaagatagg tggacatcct
       61 ttcgtttttg agagtccatc gatcccaccc tcacggaatt gacgcatcca actagcgatc
      121 aaattagggt tgttcatgtt aagtgtgttg gcaacttcac gataggacag ctcacttgtt
      181 agatataact ctatcgcatc cagcttaaat tgaacagaat attttttctt ttgtcgcttt
      241 cttactagac cctcttcacc aaattcttga taggcattga tccagcgctt cacttgtgat
      301 ggagccttaa tggaatattt ctttgccaga taatcacttc cgcctttacc atccaagtaa
      361 tcatgaacga tctttaattt aagttcaaaa gtatacttag ccataaaaaa taccgacctc
      421 caaaagttag attttttggt ctaacttttg ggggtcggta caaaacttaa aagttttaga
      481 cccttttatt ttcttaatga cttcagtcag ttttgtgcgt aaagcgtgcg aaacaagaaa
      541 accgcgcgtt tctacgcgtg gaagaagtat ctttagcgat aaaaactcct ttcgatatac
      601 tagtgagtgg ctaccaaccg ctcatcagat cgaaaggagt tttttcatgt caaatgacga
      661 taaaagttta gcatatactc gttggaattg taagtatcat attattttta caccaaaata
      721 tcaacgaaaa gtaatctatg ggcaattacg aaaagatctt ggaaaaatac ttaggaagtt
      781 gtgcgaaatg aaagatgtgg aaatcataga agcacatgca atgctagatc atattcacat
      841 gcttgttcga gtaccaccaa aaatatgtat atccagtttt atggggtatc tgaaaggtcg
      901 tagtgcggta ctaatccacg agcagcacgc aaatctcaaa tataaatacg gaaacaaatc
      961 cttttggtcg aagagatact atgtgagtac agtcggattg aatcaaaaga caaatgaaaa
     1021 atacatccgt gaacaagaag ctgatgatcg cattcgatat attataagta aaagagagta
     1081 cgtagaacca tttaaaaaat aaagagaagt aagtgagtga agatgagcag ttggcggtct
     1141 tttaaaaatc cccttttaag gggcagcgag tgtcgacccc ttctaggggg attaaaaata
     1201 cacccgtcct cacgggtgta tgggttactg atagacttga ttttaactgg tgtcagaaac
     1261 ttatgtccag aaagggtgat gaaattttgg caatttacgg ctattcaaga attagcacaa
     1321 gcaaccaaga ttacaaaaca caaattcaaa cattagaaaa tgctggcgca gaaataatct
     1381 tcctagaaaa tcactggatt atcgaacacc tttggaagta tttttgagtt acgtaagtat
     1441 tgatgatctg tctaacttaa tttgacaatt aagagtatat ttacttcaga tggtaaggca
     1501 acaaaataaa atacaatagg cagatgaact aataaataaa atctgtatta aatacagata
     1561 aattaatata aaaatcaaaa ggatcataaa cttttaacaa gtgatgaaat gggtggaaat
     1621 tgattcttat agacagtttg tctcataaca ttgaagtatt ttgcaatttt agcattgaaa
     1681 cactttttta atcttcaacg ttgttcttat aaacaagatg attgggaatc ttttgaatta
     1741 acactgtata gcttagaaca ccctttatag aaaacattcg cttagcaatc ttaataattc
     1801 cttgtgcccg atgtcgttta gattcagtag gttcctgctc atcgatcatg gaaaaaattt
     1861 gaaggatcag atccttaata aatttattta agagaggatg atccattgct tcatccatac
     1921 tcgataaact agtgataatg agctacacgt ttttctttca agttgatcaa tagcttccac
     1981 gataaaaata tctttagata aaatcaagaa ccgtaggaac tacagggata tttggtcaat
     2041 aagagccacc gctattagta aaaaataagc tatcaagtat gctctcatcc caagaattgc
     2101 ctacttcaat cgttaaaaac cgttaggttt agctaagtgg gggtagttca ctcaatactt
     2161 ttatcaataa tagagcgcaa aactttttct cttaattcaa ctgaatcaat attattgttt
     2221 tgttcataac catgaactaa tagatcacaa atataaagtt gtgatatttt tccagctaaa
     2281 gatccaccat taagaaattc ttcaatggct gtttgtaaaa ctaagtctgc agactttcct
     2341 ataggtgaat gaatatagtt agttatagca ataatttttg cgccattttt tttagctatt
     2401 ttcaatgaat cataagtatc tttcgttttc ccagataaag aaaaaatgat tactaaatca
     2461 ttaactgtta ataaagaagc gacttgggct tgaaaatgag ggtctagtac agcttttgct
     2521 tgaactccta cgcgaagaaa catattttct aaatcaagac tagtatttcc actagatccc
     2581 actccaaata tatatatgtt ttttgattga gtgatcattt taatggcttc attcaatttt
     2641 tcttcgtcta aaagcctaat agtggaatag agagcttctg ttaaactatt agcaatttca
     2701 tcatagtact ttacactagt cggatgttct tttttttgac taaagtcttc ctttgcaatt
     2761 tcaattttta aatcagaaaa accagaaaaa ccaatttttt gacaaaagcg aataatagta
     2821 gcatcgccaa cttttgtgtt tttcttaata tcactcatcg tactataaat aatagttttc
     2881 ccagaattta aaataaaatc agcgactttt ttttcagact tcgttaaaaa tggataattt
     2941 tctttaatta aatagatata actcatacaa actcctctac aatttcaact tttctccatt
     3001 atactccaaa ataataaaaa aaactcctaa atactccatt tttatcttta cttttatgaa
     3061 agcggatgct atattgtttt tggagttgtt atccaaaaat aataaaaaat tggagtgttt
     3121 aggataatgg ataaaaagag gtttttagat cttatttcaa aaggtttgat tgtttcctgt
     3181 caagcattac ctggtgaacc attttacaaa gaggaagaaa gtttaatgcc ttattttgct
     3241 ttggctgctc aaagggctgg agcagttggt atacgtgcaa attctgttcg agatattatt
     3301 gaaattagaa agtgtgtatc attaccaatg attggaatta ttaaaagaga ttatccacca
     3361 gaatctccat tcattactgc cactatgaga gaagtagatg aattagtaga aaccggtgtt
     3421 gaggtaatcg ctcttgattg cactcttaga aatcgacatg atggactatc tataaattct
     3481 tttattacta aaataaaaga aaaatatcct catcagttat ttatggcaga tatttctaca
     3541 tttgaagaag gcaaaaatgc atataaagca ggaatagatt ttgtaggaac tactttaagt
     3601 ggatatactg aggaaagtaa aaaacaagat gggccagata tagatttgat taaaaaattg
     3661 gtagaagaag ggatttgtgt aattgctgag ggaaggattc atactccaga acaagcaaaa
     3721 gagataaaaa aaataggagt tgcaggtatt gttgtgggtg gtgctattac tcgaccacag
     3781 gaaattgcat caagatttgt taaagcatta agttaggggg agaaataaaa tgtttaaaaa
     3841 attttcacaa ttaggacgcg ctttcatgtt accaattgct atattaccag tagccggatt
     3901 actattggga ctgggaggag cgttaacaaa tgagagtgct ataaatgctt atccattttt
     3961 agatcaaccg tggttgcata caattttaag tattatgagt tatgctggaa atgcagtttt
     4021 tgcaaaccta gcactaattt ttgcaattgg tattgctgta ggacttgcaa atggagataa
     4081 aggaacagct ggtttagctg gtggagtagc ttatctagta tatactgcaa ctattagtgg
     4141 gtttcttgaa cttttttcag caaaagatgc aacactagat acaggtgtag tgggatcaat
     4201 tgttgttggt ggtactgttg catttttaca caatcgttat agaaaaattg aattaccaca
     4261 attcttaggt ttttttggag gatcaagatt tattccgatt gtttctagtt tagctgcaat
     4321 cattattggt tctttctttt atttaatttg gccaccaatt caaaatggtt tagtagttgc
     4381 tggtgaacaa attgcccaaa tgggtagttt aggaactttt ttatatggtt ttttattgcg
     4441 tttaacaggt gctgttggac tacaccacac catttaccct ttgttctggt atacatcttt
     4501 aggtggtacc gaaactgtag ctggatcaac aattgctgga gcccaaaata ttttctttgc
     4561 ccaactagct gatccaaacc acactggtct gtttacttat ggtactagat tttttgcagg
     4621 aagatttgca acaatgatgt ttggattacc agcagcatgt tatgcaatgt atcgtgcaat
     4681 tcctaagaaa aatcgtaaaa agaatggtgg gctttatttt agcggggccc ttacatcatt
     4741 tttaactgga attactgaac cagttgaata tatgttttta tttgttgctc cttggcttta
     4801 tgtgattcat gcattcttag atggattatc tttttatttt gctgatatct taaatattcg
     4861 tgtagggaat tccttttcag gtggtttgat cgacttttta ttattcggtg tacttcaagg
     4921 gaatgacaaa actaattggt taaaattaat tccttttggt attgcatggg catttatata
     4981 tttctttgtt tttacattct ttatcaaaaa atttaaggta gctattcctg gaatggaaga
     5041 agaatcagaa gttcattctg aaaaagtaat aaacaaaaat acatcattac atgaagagtc
     5101 tcttatcatt attgaagctc ttggtggaaa agaaaatatt gagtctgttg aagcttgtgc
     5161 aactcgtttg cgtgttgctg ttacgaatgg ggagattatt caaaaacaaa caataaaaga
     5221 cactggtgca actgctgtct tcgaagtgaa gggtggcatc caagctgttt ttggagggaa
     5281 agctgactta ttaagtcaag aaatcaatca aatattagga aaagataatt aggaggtttt
     5341 taatatgttt gaaattttca aaaagaaaaa acaagaaact ttttatgcac cttgttccgg
     5401 aaaaatcatt tccatcacag aagtaaatga tcctgtcttt tcacaaaaat tactaggtga
     5461 tggttttgcc gtcatacctg atggttcaaa aatttatgct ccattggctg gtcgagtaac
     5521 aaatatattt cctactaaac atgctattaa tataaaaagc aactcaggca ttgattattt
     5581 gattcatatt ggaattgata cagttgaatt acaaggagag cctttttcga tttgtgttca
     5641 agaaaatgat gaggttactc ctgaaacctc gttggttgat gtggattttg acaaaataat
     5701 aaaatctggt aaagatactt ctgttatagt tgtttttccg gagaaaaatc agttcaatga
     5761 tttaataata gataatggtc gaattgctca tgggaattca tgtggaaaaa tcacttttta
     5821 aaaaagtgta tataaaaagc tataaaataa gctaaatagg atatttgtca aatgaagttg
     5881 gtgaacagaa agaactgttt tctttctgtt cattttttta ttttattagt aatgattccc
     5941 catgccaaat catgatccgt aatttaaagt tataaaagct tcga
//
LOCUS       DAHHZJ010000155         5981 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.157, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000155 DAHHZJ010000000
VERSION     DAHHZJ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.157"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            219..473
                     /locus_tag="NBL02_002390"
     CDS             219..473
                     /locus_tag="NBL02_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCD2573062.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(562..1608)
                     /gene="holA"
                     /locus_tag="NBL02_002391"
     CDS             complement(562..1608)
                     /gene="holA"
                     /locus_tag="NBL02_002391"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCD2573063.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(1646..3919)
                     /locus_tag="NBL02_002392"
     CDS             complement(1646..3919)
                     /locus_tag="NBL02_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HCD2573064.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(3912..4451)
                     /locus_tag="NBL02_002393"
     CDS             complement(3912..4451)
                     /locus_tag="NBL02_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HCD2573065.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(4587..4805)
                     /locus_tag="NBL02_002394"
     CDS             complement(4587..4805)
                     /locus_tag="NBL02_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCD2573066.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(4802..5047)
                     /locus_tag="NBL02_002395"
     CDS             complement(4802..5047)
                     /locus_tag="NBL02_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HCD2573067.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(5163..5891)
                     /locus_tag="NBL02_002396"
     CDS             complement(5163..5891)
                     /locus_tag="NBL02_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCD2573068.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
BASE COUNT         1840 a         1225 c          976 g         1940 t
ORIGIN      
        1 tagtttggtt tgacgctatt gcctcccaca atagcgtctt tttgattttt ttcagcttgt
       61 actatccgtt tttactgaaa aaaaatccta ataaaagact gttaaggaaa aaaacttgtc
      121 atgttcatat tgcaaagcac gctgccttta ggtataataa tgaatgttga attatgcggt
      181 aacacgcgta agaacttaag ttatcggagg tgaaactaat gccaaacatt gaatctgcaa
      241 tcaaacgtgt tcgtacaagc gaaaacgcta atgtgaaaaa ttcatctcaa acaagcgcta
      301 tgcgtactgc aatcaaaaaa tttgaagatg ctgtagcttc aggtgctgac aacgtagatg
      361 cgttatataa agaagctgtt aaagctattg atatggctga atcaaaaggt ctaatccaca
      421 aaaacaaagc taaccgcgac aaatctcgtt taagcaaaaa aatcgctaaa taaaagaatc
      481 ggcttcggcc gattttttta tttgtcaaaa aaaggacaaa aacaaacgac cttcccgcat
      541 gctttgccct cgtctttctt cttatcgatt tcttaacaac tgccctgtgc gcaaaacgaa
      601 tagttgaaaa cttaattctt tttcgatttg tcccgttttg atttgataat ccatctcaac
      661 taattgatca aacaactgta ccagcaaatg ttcatcgaat ttttttactt cctgcatggc
      721 aagttttaca cgatatggat ggattttcaa tgtttcagca atatttgcct gctgataacc
      781 aatctttaca agaaattttg tctgtaaaaa taaacgtaat tgagaaagca aaatcgcatt
      841 aattttgatt gtttcttccc cttgcataac taaatcttcg tataaacgca aagcttgttc
      901 cgtcttccct tttaaaatat attgggtcat gtcaaaaata ttatgttcta acgttttagg
      961 aactaattct tctacttctc ttaccgtgat ttgctgttga ttttgtccat aaaggaccaa
     1021 tttctgtaac tctctcatca ttttggataa atctaagtcg gtcaacctca agaataaatc
     1081 aattgcactt cgttccattt tgattgagga attctctaag gtattcatca gatattgacg
     1141 tacttctttt tcattcaact gttggacatt gatgataatt gctgtttttt tcaattgttt
     1201 cgttactttt ttccgttcat ccaacttttc atatggcgca aagaatacta atacagtcga
     1261 ttctaatggt tctttcaaat aagccgtcaa taaatcagta ttttgttcga ttccattgtt
     1321 tactttttca cctgttaaaa aataaggatt ctctacgaat actaaccgtt gatctccgaa
     1381 aaacggcaac gtctctgctt cagctactac ctcatctaaa cttgcatttt ccatgtcaaa
     1441 acttaaaaaa tttaagtcat cttcgccctc taattggatt tttttgacaa tttctgtccg
     1501 aacttgatct tggagaaact tttctgttcc taaaatcaag taacaagatg caagtttttc
     1561 tttacgtatt ttttgtaact ctgcttgtat actcaaatta ctcaccactt tcaaaaatta
     1621 tcctaccatg agcagatgag aattactagt ctatcattgt ttccactagc ccatgaggat
     1681 gaaaaactgg atgccagcga taggtgatca ttccttgttg atccgttcgc catgttttta
     1741 tctgattttc ttctagtgtc tccaatactt cgctgtgcgg gtgaccataa cgattattta
     1801 cgccgcagga tataatccca tgttttgctt caatatggga aagaaaatct gtgttacttg
     1861 acgtacggct accgtgatga cctaatttga ctacatcggc tttcagatta ggataaatca
     1921 aaagcaattg tttttccatt gcttgattca aatctcctaa aaataaaaag cgcctattgt
     1981 tatattccat ccatagacca atcgaatctt catttcttcc ctctcccttt tcaaaaggag
     2041 tcaatacata aagatcataa taaccgctga tcctgtcccc atgtttgatt tcaatgatct
     2101 cagtaccatt tttttgagct tcagataaat gcctttgtaa tggctcattt cgtccagcgc
     2161 cccaaccgat ataaattttt ttgatagtaa aatgctgaga taacgtgggt aattctccca
     2221 catgatccgt atcgtcgtgt gtcaagatga gtttatcgat tacacttatc ccctgacctt
     2281 ttagatatgg aacgatattt ctttcagcag gaggacgtat cataccttgc gcccactttt
     2341 ctctttcaaa gtgtaactga cctccagtat cgatcaaaat cgtttctttt cgaaaaggac
     2401 tttgcaaaaa gatactgtct ccttgaccga catcgataaa agcgatcgtt gaagaaaagc
     2461 gtaaaaatgg caaagtcaaa aaaataaaag aaaaaagacc aacataaaac aagtgtttca
     2521 caggttgttc tttcaaccta tccatacaaa tcagtaatcc gaaaatatac atcccagata
     2581 gcacaagggg caaacgtcct ataatgagag acgaccctga agaaaaatcg attattcgct
     2641 caaataagat taatccctct tctatcagca aaagtaaagt ttccggcagc cagcgattga
     2701 agagtgtgag aaataacaca ccaggaagaa aaagaaactt gaataccggt gcaatcagta
     2761 atgtaaaaag actacctaaa ataggccact cataaaaatg ccatacaatc aacggcatcg
     2821 tgatcatagt aaacaggacg tgagaggccg tttttttcaa ccaatctcca tcaaaagaaa
     2881 tcaggataaa caaaaaactc atgaaaaaac taagctgtcc acccgcatga aaaagaaatc
     2941 gaggatcaaa tagtaaaaga agccaaaaga caactgcaaa taagtccatg gtggaataat
     3001 gcttattcag caatttgact agcaatcgca ataaaaacag caaactagca cgcaaaacag
     3061 aaatagaact tcctgcaata gaaacataac agatagttag tagactcaac ggaataatac
     3121 tgacgtgtag tggccatccg atacgtcgaa ataaataata cagccaccct aaataaaatt
     3181 ggatgtgtag acccgataga ctgaacagat gcagcaaacc actctcccta aaaatttctt
     3241 ctggttctct gaatcctgca tctttatagc cccataataa agaattaata tatacacttg
     3301 ttttttgcgg aaattcagat tggacaaagg agataaaaga tcctcttttt ctctgaagcc
     3361 agtttcgcca gtgattttgc atggtgatct gttcaatctt ttgaatttgg aaccgtccag
     3421 aaattctatg gttcctgaag taagcttctt ggtcaaaatt ataaagattc cgcttttttg
     3481 ctggagagat gaactcgcct tcagcagtca attctattcc ctttcctcgg aagttaagcc
     3541 atgttttcaa ttctgcttct gtgtttactt gataagaaag ctggagcttt tgtttattct
     3601 ctgataacct tccttccatt gtcaaaaaat taccatttgc acgaatcgta tctgtaaata
     3661 cagtaatttg cgcagatgtt gttttttcct ctaaaaatga atactcttgt agcctataag
     3721 aaatggatac cgttatgcta cacaaaatac acaagtacgc gataaaacgt ccttctttta
     3781 gcgaaaacca taaaaaaatt ccggcaagaa gcaaacgata accccaattg tcagtgtaat
     3841 acaacaaaca gataatcatc gacgtaattg ccggaaaaat caaccgattt cgaacatgcc
     3901 tttctaaaac gctagacatg ttttcctgtt aattcatttt gaagttttga aaaatgctgt
     3961 gagtctaaag caacttggtg tacagataca cctaattgtt cgatcaacgc ttgggcataa
     4021 ggatcatttc gatagtcatg gagataattg atccctttca caccagcttg caacaaagcc
     4081 tttgtacagg ccaaacatgg aaaatgtgtg acatagattt ctgaaccttc cgtcgggata
     4141 ccaagttttg cacactgtag gagtgcattc atctctgcat ggatcgttcg caagcagtgt
     4201 ccatccacga cgtaacaccc ttcatctatg cagtggacat ctccactgac tgctccatta
     4261 tagccgccag caatgatcct tttttctcga acgagtgtcg ctcctacttc taaacgtgta
     4321 cacgtactac gtaaagataa cagtactgcc tgagccataa agtactggtc ccaaggaatg
     4381 cgttcattat gtgacttatc agtaactttt tctttattcg tatcgcatgc ttttgaagat
     4441 ttttctaaca tgttttatat tcctccgttt tttcaagttg attaaaaagt actcattgct
     4501 taaacaaaat attctttaaa tataacgaat catcctgtct gacaaaagat cctacccttc
     4561 actgccgaaa tgaagtccgt catccattag cccattataa aaattcttcg aatccatttg
     4621 ttcaagaaat tccgtataaa aatcgatact tcacaatcat ggcgttttgc tacttctgtg
     4681 agaacagaga tatattcttt gatcaacttg ttattccgat atttttgttt tgtctcatcg
     4741 actgctggtg gcgtgataaa gaaagcatga tcagaactat agtctttttt cagctgaact
     4801 atcatccact gcagattttt tggtacgttt ctttagaaag ttgtttatgc tctgctagat
     4861 cattcgtccc aatgagaata aatactttat ctgctgtata gctaagtaca tctttggcaa
     4921 agcgttctcg tagagcttga ctgttattgc cgctgacagc cgtattcata atatgaatcc
     4981 cagatatttt attttggaga tagacattca gcattggttt ttttaatgtt tctttgcgaa
     5041 caatcaagct atttcctatc caaaaatact acctcctcgt cctgccaagc tcttagaatt
     5101 ggattattct tagtatccat tagcgtccat ttattgctcg tgccagtcga ttttgaatca
     5161 ttctatttaa ttgtaacgaa gttttttagt ttttcaactg tcttttcacc gataccagaa
     5221 atctcctgca ggtcttccac tttctggaag gatccgtttt cttctctaaa tcgaatgatt
     5281 tcttgcgctt tgactgatcc gatcccagga atctgctgaa gttctgatgg agtggctgta
     5341 ttgatattta ttttgggagg ctcggaatct gctgtaggct gttttccttc cgaggatgcc
     5401 aactgatcaa gatgttctgg cagctcttca cctacagcag ggatatatag gacttgctga
     5461 tcttctatct tcgcagaaaa gttgattcga tcgctatccg cttcttctaa taatccacct
     5521 gcacgcttta gtacatcata gacccgttct tctgaagaaa aggaatagat accaggctcc
     5581 ttcacacttc ctttgatatc ggtgtacatg acctttggac gagcatcctc ttctacatcg
     5641 tttccttcag ctgatggatc ttctccactg gaactaaacg aagaaagtat ttcttttgca
     5701 gcagtctctt ctgtttctct ataaaatagc aaatttgata ataaaaaacc acttgcgata
     5761 caaaagataa gaactatcat agacatccaa atgatcaatt tgggtgaagc tagtattttc
     5821 ttgctgattt tttctatcat tgttcttttt tcaaagaatg accagtaaaa atcattcatt
     5881 actcctttca tttaaaattg aagaaacagt gagctctggc aaaatcgtcc cttcaacatc
     5941 taattacgtc aattttttct tttgttttta tttttatcgt g
//
LOCUS       DAHHZJ010000156         5908 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.158, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000156 DAHHZJ010000000
VERSION     DAHHZJ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.158"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(<1..942)
                     /locus_tag="NBL02_002397"
     CDS             complement(<1..942)
                     /locus_tag="NBL02_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCD2573069.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISI"
     gene            complement(944..2383)
                     /locus_tag="NBL02_002398"
     CDS             complement(944..2383)
                     /locus_tag="NBL02_002398"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCD2573070.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2568..4514
                     /locus_tag="NBL02_002399"
     CDS             2568..4514
                     /locus_tag="NBL02_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HCD2573071.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            4778..5650
                     /locus_tag="NBL02_002400"
     CDS             4778..5650
                     /locus_tag="NBL02_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCD2573072.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         1862 a         1016 c         1053 g         1977 t
ORIGIN      
        1 tattgagatt ggtaaaatat aatccttatt tgtcttttcg cctttaattt ttttcagaag
       61 aatatcaaca ataagacttg caatttcatc aattggttgt cttattgttg tcaattgtgg
      121 gaaaaatttc tcaataaaag aggtaccatc gtatccaata atttttaaat cttctgggat
      181 attaagttta agttgatgag caattttcat agtgagaatg gctgttaaat catcagaaac
      241 aaaaatacca tctggtttgt gatttataat agttgacttt atttccattt cacgacggat
      301 agtagacaga ttattaggaa ttttgaaaat ttttccattt ggattttgaa aagaaaaacc
      361 tagtgcacgt aattctgtgg gagaatccgt gttatcattt cctgtaatca taatggtatt
      421 ttgacaacca tttttttgta aagtcttagc tgcaagtttt cccccttcaa agttatctga
      481 agaaacaatg gaaacattag gagctaaatt tctatcaaaa gcaacaatag gtgctctcac
      541 acgctcataa tcagcaattc caagattatg gcttgatgag attatgccat caacttggtt
      601 ggcttcaagc atttcaatgt attctctctc tttagcagga tcattttgac tattgcaaat
      661 aatcgtttta tatccatgtt taaataactc tatttcgaga tactcaatga gttcagagta
      721 aaagatatta ctaatattag gaaaaataag accaataagc tgaggtgact ttccttgaag
      781 tccacgtgct aaattatttg gtttataacc aagagttttc atagcttcat gaactttttg
      841 ttttgttttt tctgataaat atcccttatt attaatgacg cgtgaaacgg ttgttgggct
      901 aactccagct aattcagcga catcagttaa ttttgctacc attttaatat tttaattcaa
      961 agtattttcc agtaggttcg cctgatttga tttgaatacc attttggtga ttttcaggga
     1021 atacacgact agtaaacact ttttctcctt tattaataaa aatctcaatg attgagttat
     1081 ctacaaaaat gttagcagtt gtttctttag tatcaattga gcattcacga atcgtaccaa
     1141 agtcagtggc atattgaatg cctgctttgc tgcgatctaa aataattttt ccttctttgg
     1201 tatctacaat caagctaagt ccatttcctt tactatcaga gaaaagaagt aactccgatt
     1261 tctgatttgg aggaaaagtt agttcaagct cataagtatt atttgtttct gcttttgctg
     1321 taaattgttc agaactagca cgtaatgatg taatagcttc tactggatat tggtaaagtt
     1381 taccatcttt tattgaaagt tcttttacaa gactcattgc tccctgataa tcatacttat
     1441 cagttggata atcgatatca ggtagtccaa tccaactgac aatcagagca cgaccatcag
     1501 gagtattgaa tccttgagtc gcatacgctt caaatccata atcaagattt tgaatttctg
     1561 aagcgcccac aagttttact ttttctatgt caagcgattg acagatctta aaagtattcg
     1621 gatagatatt cccataattt agctcagatt tatccaaccc ttgaggacaa taaatgagaa
     1681 caggtttttt gtctacaaat actagatttg gacactcaat catgtattct gaaccagtgc
     1741 cgccaaaatc aaggttgcca acttcctccc atgtttccac gttattgtca tccgctttgt
     1801 aaagtttgat aaagcctgat ttttctaaat tttgtgctcc aacaatcgca tacaattgtc
     1861 cttcataatt aaacatttgt ggatcacgaa aatgatcagt tacatcagca ggtttatgaa
     1921 taagaacatt ctcacctttc gtaatgttgt agtctttatc catccatgct ccgatttgaa
     1981 gtgtatcacg ttcccaattt tcatttcgaa cgtttcctgt atagagtaag aatagattcc
     2041 cctctatttc atatgcagaa ccggaataag cgccgtggct atctcgtttt gtgtctggaa
     2101 gaagttctgc tcctgttaca tgaaaatgaa ctaaatcatc tgattcattg tgtacccatt
     2161 ctttcaaccc atgagcagct ccaaatggcc aattttgata aaaaagcgta tattttccgt
     2221 taaaaaaaga aaaaccatta ggatcgttta acaatcctgt tttaggttcg atatgatagc
     2281 tagcacgcca aggtgatcgt tttacattat ccttaatttt atccatctca tctttagtcc
     2341 aatcagaata agcacgatat cgtatttctc gaggtaagtt catattttac tccttcaatt
     2401 tatcttttta caactagtat ggtaaacgtt ttccgttgaa aaatcaatca tttcatttaa
     2461 aaaaataaac tatatgacaa acgtttgaca tatttttaag atatgctaaa ataaatttgc
     2521 atttcagtga accgttttca cgtaaaaata aaaaaggagt gattcgaatg aattacagta
     2581 aagtagcttc cgacgtaata caagcggtcg gaaaagataa ccttattgca gctgcacact
     2641 gcgctacacg tcttcgtctc gtactaaaag atgacacgaa agtgaatcaa aaagctttag
     2701 acgaaaatcc agatgtgaaa agcacattta aaattgatgg acaatatcaa gtgattatcg
     2761 gtgcaggtga tgttaatttc gtttatgacg aacttatcaa aaaaactgga ctttcggaaa
     2821 tttcaacaga tgatttaaaa caaattgcta gtaaagataa aaaattcaac cccattatgg
     2881 cattaattaa attattatca gatatttttg ttccaattat acctgccctt gttgcaggtg
     2941 gtttattaat ggcattacaa aactttttaa cagctgctgg actttttgga ccaaaatcaa
     3001 ttgaagaaat gtatcctgcg attgaaggta tttcctcaat gatccaactg atgtccgctg
     3061 caccattcat gttcttacca attttagtag gtatttcagc tgctaaacgt tttggagcaa
     3121 accaatttct tggtgctgca attggtatga tcatgacaac accagatctt ggtggctcaa
     3181 ctgaatattg gaatattttt ggttatcatg tttcacaaac aaactatgct tatcaagtta
     3241 ttccagtttt agcagctgtt tggatattat ctatattaga aaaattcttt cataaaaaat
     3301 tgccatcatc tattgacttt acattcacgc cattattatc agtaatgatt actggatttt
     3361 tgactttcac agttattggc cctgtaatgt tattgttgtc taatggaatt acagacatta
     3421 ttgtttggct atataacact actggattta ttggaatggg tatttttgga ggaacatatt
     3481 cacttatagt catgactggt ttacatcaat catttccagc tattgaaact caattacttt
     3541 cagcgtggac aaatggtatc ggacatggag actttatctt tgtagttgca tctatggcaa
     3601 acgttgctca aggtgctgca acatttgcca tttggttctt aactaaaaat tctaagacaa
     3661 aaagtcttgc ttcctctgcg ggtgtctcag cacttcttgg aattacagag cctgccttgt
     3721 tcggagttaa cttaaaatac cgctttccat tcttttgtgc tttaattggt tcaggaattg
     3781 ctacagcagt agcaggatta cttaaagtag ttgctgtttc acttggttca gcaggtttct
     3841 tgggatttct ttcaattaat gcaacatcta ttccatttta cgtattgtgt gaattgatta
     3901 gctttgtagt tgcttttgca attacttact tctacggtaa aacaaaagct gctggtatct
     3961 ttaaagcaga agaaattgct gaacaaacac cagttaattc ttcaaaagta gcaacaacag
     4021 atccaatatc tactgatgtt gaatcaacac aaacaaaagt ttcatcagaa acaattgtta
     4081 gtcccatttc tggtaaagtg attagtcttt cttcagtaaa tgatccagtc ttttcatcag
     4141 aagctatggg aaaagggatt gccattaaac caaatggaaa cacggtccat tctccagtag
     4201 atggtactat tcaactcacg tttgaaactg gacatgctta tggtattaaa tcaaataatg
     4261 gtgctgaagt tctaattcat attggtattg atactgtatc aatgggagga aaaggattta
     4321 cacaaaaagt tactactaat caaaaagtta gaaaaggcga tattcttgga gcatttgacc
     4381 gttcaatgat tgagaaagaa ggtcttgatg atacaacaat gattattgtg accaatactt
     4441 cggattattc agaagtaaat atagtagctg aaaatacagt cactgagggt actgatttac
     4501 ttgaaatcaa ataaatatat ttccctgagt aaagatggaa ttttctttcc aaaatcaaat
     4561 aatatagatt aatcaaatat ttgagaataa cattttattt aatcttttca tatatcaatt
     4621 gtatgaaaaa ttattagaat tttaaaaaat gataaaaatt caaaaagaaa aaatagtagt
     4681 tatttacttg tcatgttact aaataaaaaa ataatttaaa tatgatatat agtcatttat
     4741 ttagtgattt cattctaaaa taaagggaga aatgtctatg ctatacggtg caattgaagc
     4801 aggcggcaca aaatttgtgt gtgccattgg cgatgaagaa atgacgatca aggaaagagt
     4861 gagctttccg acaacgacac ctgaggaaac aatgccttta gtcattgact tcttcaaaca
     4921 ataccaagca gatttagcag ggattgggat tggctcattt gggcctatcg atatccatcg
     4981 cgattccgcc acttatgggt acattacctc gaccccgaaa ttggcatggc aaaattttga
     5041 tttcatcggt accatgaaaa aagaatttcc gatcccgatt tcttggacaa cagatgtcaa
     5101 tgcggctgcc tatggtgagt atgtcttcgg cagtggcaaa ggattgagca gtgtcgttta
     5161 ctatacgatc ggtacgggtg ttggcggcgg tgccttacaa gaagggcgct ttatcgaagg
     5221 gtttagtcat ccagaaatgg ggcatatgct cgtcgtccct catccaaaag atgactttgc
     5281 tggcagctgt ccatttcatg gcaactgttt agaagggatg gccgctggac ccgccatcga
     5341 aaaccgctta gggaaaaaag gacaagaggt tgcagaagat gatccttatt gggaaatcga
     5401 agcctcctat attgctcaat gtgcctacaa tacaaccttg atgttttctc cagatgtgat
     5461 catttttggc ggtggtgtga tgaaacaacg ccatatgctg caaaacgtcc acgacgcatt
     5521 tgcaaaattg gtcaatgggt atgtcgaaac acctgagtta agcaaatata tcgtgacccc
     5581 agcattagaa gacaacgctg gaacgctcgg ttgtttggca ttggctcgag aagcggtctt
     5641 acattcataa acgaaaaaag gctcttcgac atttagtgtt ggtagagaaa gatacaactg
     5701 aaaatttagt cataaccagt taggcagagt gcttaactgg tttttgtttt acttggaaga
     5761 tcaagccttg aatgagataa attcttgctc gaaagttatg gaaatttcga tagccgtagg
     5821 ccacttgtat tgtgcaatag ttttttacaa aaaaaagggg gtgcggatat ttttttggac
     5881 ctttttaaca aatatatctt tgcttctt
//
LOCUS       DAHHZJ010000157         5863 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.159, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000157 DAHHZJ010000000
VERSION     DAHHZJ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.159"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(99..1229)
                     /locus_tag="NBL02_002401"
     CDS             complement(99..1229)
                     /locus_tag="NBL02_002401"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCD2573073.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="NBL02_002402"
     CDS             complement(1878..3080)
                     /locus_tag="NBL02_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCD2573074.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="NBL02_002403"
     CDS             complement(3140..3343)
                     /locus_tag="NBL02_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HCD2573075.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="NBL02_002404"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="NBL02_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4880..5692
                     /locus_tag="NBL02_002405"
     CDS             4880..5692
                     /locus_tag="NBL02_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD2573076.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1735 a         1210 c          938 g         1980 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttc
//
LOCUS       DAHHZJ010000158         5845 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.160, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000158 DAHHZJ010000000
VERSION     DAHHZJ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.160"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            129..2282
                     /locus_tag="NBL02_002406"
     CDS             129..2282
                     /locus_tag="NBL02_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HCD2573077.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            2251..3666
                     /locus_tag="NBL02_002407"
     CDS             2251..3666
                     /locus_tag="NBL02_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCD2573078.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            3699..4733
                     /gene="sppA"
                     /locus_tag="NBL02_002408"
     CDS             3699..4733
                     /gene="sppA"
                     /locus_tag="NBL02_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCD2573079.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            4735..5256
                     /locus_tag="NBL02_002409"
     CDS             4735..5256
                     /locus_tag="NBL02_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HCD2573080.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            5253..5639
                     /locus_tag="NBL02_002410"
     CDS             5253..5639
                     /locus_tag="NBL02_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCD2573081.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
BASE COUNT         2095 a          785 c         1105 g         1860 t
ORIGIN      
        1 tgaaaaagtt agtgttcaag aactgttttc aaagtttttg tggaattatg agacaatatt
       61 ttcactgtat taaagctata ttgagaattt ttctataaaa attttttaga aggagttgtg
      121 ttagataagt gataaaatca tttccttttg tgcagcaaca agatgaaaaa gattgtggtg
      181 ttgcttgcct ttcgatgatt ttaaaatatt ttaaaactga tatcccgatt cataaattac
      241 gggacctatc tgggactgat ttagagggta cttcagcatt tggattgaaa aagacatttg
      301 aaaaattgtc ttttgattgt atggctattc aagcagatag tgagatctgg aaagaaagag
      361 atcttatctt accattaatt gcacatatat tggttgataa cagctatatg cactacgttg
      421 taatttataa aacgaaggga aacgtactat ggattgcaga tcccgctaat ggaaaaaata
      481 gaaaaacgat tgaagcgttt caaaaagaat ggacaggtat tcttttatta cctatgccta
      541 gagaaactta tattcctatt aaagaaaaag tagctggatt aagttctttt ttgcctataa
      601 tttggaaaca aaaaggatta gtattccaca tcgttttagc atcgattttc attacgtttt
      661 ttggcatagg aagctcatat tattttcaag gtattcttga ttttttcatc cctaatcaag
      721 cacgttcaac tttaaatata atttccattg gcttgatcgc tgtctattta tttcgggtat
      781 tgtttgaata tagtcgtagt tatcttctta ttattttagg acagcgaatg agtatggcaa
      841 ttatgcttca gtatttcaaa catgtgttaa ctttaccaat gaattttttt gctacgagaa
      901 aatcagggga aattatttct cgttttcttg atgccagtaa gattattgat gctttagcaa
      961 gtgctacgtt atcaatattt ttagatattg gtatggtgtt gcttgttggg gttacgttag
     1021 ctattcagaa tagtacgttg tttttaatca ctctatgctc tttgcctttt tatgtagttg
     1081 caatattcgc tttcgtgaaa agctatgaaa aatcgaacag ggaagaaatg gcagccggtg
     1141 cagctgtaaa ttctagtatc atagaaagtc ttaaaggaat agaaacaatt aaagcatata
     1201 gcggtgaaga gaaagtatat aaccgtatag accatgaatt tgttaaatta atgaaaaaat
     1261 catttcgtac ggttacgctt gataatattc aacaaggaat taaacatttg attcaattaa
     1321 ttagcagtgc attaatttta tggctaggtt cttattttgt tatggataat agaattagtc
     1381 taggtcaact tatcacatac aatgctttac tagtattttt gacagatcct ttacaaaaca
     1441 ttattaattt acaagtgaaa atgcaaacgg ctcaagttgc aaataatcgt ttaaatgaaa
     1501 ttttcgcgat tgattcagaa aaatcgaacc agtcttttga atatatgctt tctgaacaat
     1561 tatttcaaca agatatcact gtaaaaaacg tttcattctc gtatagcatg aaggccccaa
     1621 cgttgaacga aatttcatgt acaattcctt cgcacagtaa aacggctata gttggagtta
     1681 gtggttctgg aaaatcaact cttgcaaagt tatttgttcg tttttatgaa gcatcagaag
     1741 gatcgatcca ttatggaaac attaatcact tagacattcc ttttcagata ttacgcgatc
     1801 gagtgacata tttgcctcaa gagtcatttt tctttagtgg aacaatcatt gagaatctta
     1861 tattcggttt agatagccca ccaagtttcg aacaaataat agaagtttgt gagatagtcc
     1921 aactaaatga atttattaat caacagccat tacgttacga gacattgttg gaagaaggtg
     1981 gaaataatct ttcaggaggg caacgacaac gattagcctt agcacgggct ttattaaaaa
     2041 atccggacat attaatttta gatgaagcaa ctagtggatt agataccttg ttagaacatg
     2101 caattatgaa ttatctcctc aatttaaaag aaaaaacgat tatttttatt gcgcatcatt
     2161 taccaatagc aaaagcatgt gatcaaattt tagttctcca tgaagggcat ttggtagaaa
     2221 aaggaacgca cgatgaactt cgttttgccg atggtatcta tcaaaaatta tgggaaatct
     2281 aggagaggga aacaatttat gcagaataat aattggttcg aacattctag tatctatact
     2341 aaacaacatc atagcttcta ccgttgggtg ttataccctg taattctttt tttatgtatt
     2401 attggattgt ttttaacctt tgctaaaaaa gaggttgtta ttcggacaac ggctcaattg
     2461 aaccccgaaa aaatagagaa gttacaagtg ccattagaag ctaaaataat ggaaaacttc
     2521 ctaaaagaaa atcaatttgt tcataaggac gatgtgttgg ttagattgga ttgttcttta
     2581 atagagaatg aaaaggctca gatagaacaa gaaaaccaac gtattactca acaaataaag
     2641 atggctcagc tatttattga aagtataagt aaaggaaaaa atttgttttc aacggatgac
     2701 agttttggct acagtaatca attaaagagc atgttgtcag aaaaagaatc actccgctac
     2761 gctttgaagc aaagtgaatt aaatgatcaa aagcaattag aagtatacga aaagacaaaa
     2821 agacaactag aaaaacaaat tgagagttca gatagtaaat tacaagaatg gcaacaagta
     2881 caggtagctt ggagtaataa tcaatcatta aaagattttt caaaagaaat gatggcaaac
     2941 tatgagaatt ggcaagaaca actaaataat gtttctgatg atcaaaaaaa tcaagtgaaa
     3001 ctgacaattt cagcaagcat aaatgaacaa attgagcaac taaaaaaaga agtagaacag
     3061 tatcagtcag aaaaagctaa attagttaaa ccaactactt ctgagaatga cagaattagt
     3121 caaacggaaa aaggaaagca agagctagaa caagcaataa caactgtaaa acaaacagtt
     3181 acagagctaa atgaaaaaca agagaagaac cagtcagtta tcaaaagttt ggacgatcaa
     3241 ctttcaaaag gaatactaaa agctcctgta tctggaacgg ttcatttaaa tgaagaaaca
     3301 aagggacaag cagaactagc caaaggaaca gtgcttgcag aaatttatcc gatccacgaa
     3361 aaatcaaaaa tgaaatttac agcactttta cctgcgaatg aaagtaccta tattaaagaa
     3421 gggatgaagg ttcattttaa attggatcaa aaaggtgggg ctccgattac gatcgacgga
     3481 actttagatg aaatttcaga aaattctact gccactgaga aaggtgtttt ttatgcagtg
     3541 aaaggaacgt taaaaacaac tcaatcgttt ccgtttcgtt atggattaac tggagagttg
     3601 tctttaattg taggagaaaa aacatattgg caacaaattg tcgatgtgtt tctaaataaa
     3661 aaataattat ttttactaag aatataggag gagtttatat gaataaaaga agatggattg
     3721 ctgtgggagt agcgtgcgga ttattgttac tatccattat cgtcgcgcta attccaggaa
     3781 aagataaaga ggaagcatca aatacaaccc taacaggaat aaataagatt ttttacggat
     3841 ctaacgaaat cacagaagaa acgttagaag aaggcgcatc aaataaaaag attgtcaaac
     3901 tatccgtcaa tggagttatt gcagatacag gtgaaagcaa tctttttagt agggaacaat
     3961 ataaccatca gaatttttta acacagctaa agaaaattca agaagacaaa gcggttaagg
     4021 gagtgttatt agaagtaaat tcacctgggg gaggaatata tgaaagtgct gagattgcta
     4081 aagaaatggc aaaaattaaa aagctagata ttcctatcta tactgcattt aaaaatacag
     4141 cggcaagtgg agggtactac atttctgctg gttcagataa aatatttgct acggaagaaa
     4201 caacaactgg atcaattggt gtcattattt ctggtttaaa ttattctggc ttattagaaa
     4261 aacttggcgt aactgatgcg acgtataaaa gtggtgcatt aaaagatatg atgtcacctc
     4321 aacacaaacc ttcagaggaa gagaataaag ttatacagga atttgtgatg agtgcttatg
     4381 atcgttttgt caatgttgta gcaaaaggaa gaaatatgga tactaatgca gtgaaagaac
     4441 ttgcggatgg aagaatttat gatggtaacc aagcagtaga aaatgggttg gtggatcaaa
     4501 ttggttactc tgaagatgca ttagattccc taaaaaaaga gaagaaatta actgatgcga
     4561 cggttataga atataaaaat gacacaactg gctttgcttc ttcttggtta ggtaataaga
     4621 ttgctgaatg gcaagggcta aaggcaacta catcagatcg tgtaatttca gtttttgaaa
     4681 aacttggatt ttctgatact ccaaaaccaa tgtattacta tggagggcta taaaatgaat
     4741 aagaagagtg gcattgaaac tttgcctaat tatgtatttg cgggattttg gttacgattt
     4801 tttgcttttc ttattgattt aatttgtatt agcatcatca cccatgctac tataggtctc
     4861 ttttacaaaa tcatgggact ggaatctgac aaatccatta ttacttttta tggtatagcg
     4921 tcattagcta tttatttaac ctattttgta cttttgacta agctgaattc aggacaaaca
     4981 attgggaaaa tgatttttgg gataagagta gtgagctttg aggaaaaaga gctaagttgg
     5041 caaactatct ttattcgtga gtgtatatgt aggtttatat taaaaaattt tgtatttcaa
     5101 attgggtatt tggtggctgc tttcacgccc aagaaacaac atattgggga ttatttttct
     5161 aacacgtcag tggttgttat tcgttcatta gaagctatta actatggtca aaaaatttct
     5221 aattctaaaa cacttaatct ggagagaatt aaatgaataa gagaaaaaca gagtttattt
     5281 tgattttaat tagtggaata acagggattt tatgtagtct tttagcattg atgggtggta
     5341 tgtttctatc ttctattgat tcaactactt taaatactac agaactaacc agtaaggata
     5401 tagatatgtt agtcaatcta agcgggaatg ttgttttatt tggaggaatt accctagttt
     5461 tttcgataat gattttcata ataggttttt tagtaagaaa aaatcaaaaa acagtggtct
     5521 atggtattag tttactcatt ttatctatta ttccattttt tcttttaaca ttattgtggg
     5581 taattcctgg agttttaggt attattgcgg gattgatgct tctaataaga aaatcttaat
     5641 taggcaatgt gtagacaacg tgaaacgatt aatcaattaa aagctaaata cgttttgaga
     5701 aatgataact tgtagtcgat ggaagacatg tagataaaaa aactacgtgt cttttattta
     5761 ttctaatttt ttcgaaaaaa agacactctc tgattgttgt attaatggaa aaggggaaag
     5821 cagcaagtaa atttagtttt tctat
//
LOCUS       DAHHZJ010000159         5822 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.161, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000159 DAHHZJ010000000
VERSION     DAHHZJ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.161"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(49..507)
                     /locus_tag="NBL02_002411"
     CDS             complement(49..507)
                     /locus_tag="NBL02_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCD2573082.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(520..1851)
                     /locus_tag="NBL02_002412"
     CDS             complement(520..1851)
                     /locus_tag="NBL02_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HCD2573083.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1862..3304)
                     /locus_tag="NBL02_002413"
     CDS             complement(1862..3304)
                     /locus_tag="NBL02_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCD2573084.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3291..4055)
                     /locus_tag="NBL02_002414"
     CDS             complement(3291..4055)
                     /locus_tag="NBL02_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HCD2573085.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4159..5034)
                     /locus_tag="NBL02_002415"
     CDS             complement(4159..5034)
                     /locus_tag="NBL02_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCD2573086.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5288..5372)
                     /locus_tag="NBL02_002416"
     tRNA            complement(5288..5372)
                     /locus_tag="NBL02_002416"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5336..5338),aa:Leu,seq:caa)
     gene            complement(5390..5461)
                     /locus_tag="NBL02_002417"
     tRNA            complement(5390..5461)
                     /locus_tag="NBL02_002417"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5427..5429),aa:Gln,seq:ttg)
     gene            complement(5471..5543)
                     /locus_tag="NBL02_002418"
     tRNA            complement(5471..5543)
                     /locus_tag="NBL02_002418"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5508..5510),aa:His,seq:gtg)
     gene            complement(5605..5689)
                     /locus_tag="NBL02_002419"
     tRNA            complement(5605..5689)
                     /locus_tag="NBL02_002419"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5653..5655),aa:Leu,seq:caa)
     gene            complement(5710..5780)
                     /locus_tag="NBL02_002420"
     tRNA            complement(5710..5780)
                     /locus_tag="NBL02_002420"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5746..5748),aa:Cys,seq:gca)
BASE COUNT         1765 a         1247 c          892 g         1918 t
ORIGIN      
        1 gtaaattcac ccataaatat caccctattt aacttttttc atcgtacact aaaattcaaa
       61 acaactatct ttaatcttta cttcttggtt ttgttttaat aaatttcctt caaaaatgcc
      121 tggagaaact acaatgatca ccgtatcttt ttgtgcttgc tccaacttct tcaaatctat
      181 aatagctaac agtgtccctt cttttacctc ctggttactt tcaacccgta tctgaaacgg
      241 acttccttcg agttcaactg tatctaaacc catatggatc agtatttttc tgccagattc
      301 actttcgatc gttatggcat gtcgggtagg aaatatatta tcgattattc ctttaactgg
      361 cgcatggata tatccatcat gattcgttat agcgtatcca actcctatca tttttgaaga
      421 aaagacctcg tcttttactg ctgttaatgg aataatttga ccactagcta cagcttttag
      481 aatgtttctt gttttaaaga aatgcacttt ttatcacctc tactttaatg ctggccaata
      541 ttttttgttt actttgatca tttcatctag tatttttttt gcgactctgg catttggtac
      601 ggtttgattc aatgtaaatg cttgtaaagc tttttgataa gaaccttcaa agtaagcatc
      661 tactagtaac ttctctactg caacttgtgc ctccatcaat cctttatgaa aatcactgat
      721 tggttttctt agtgtaattg gctccacacc agttgcacca acatatgccg gtatttcaac
      781 aactgcatct gaccgtagat tcggaatcgc cccttgatta ggaacgatta gcataaagcg
      841 acgacgatca tcattcaaca aggaaatagc tatatcgacg atatattgtc catgctcccc
      901 aaaattaaag ttcaatactt cttccacaat accgcggcgt attttatcag ccatttcttt
      961 ggtatttttc tctcgtccat ccatgaccat attagcacgt gtatactctt tgtcagtatg
     1021 ctcaacgacc atatcaggat ataagtaata ctctaagtaa ttattcggga tattacttgg
     1081 aaaatttttc gtgatgactg acatcatttg aaaggttttt tgccaagttt tatctcctat
     1141 attaaaatcg gcgacttgca tttcttttgt tattagtttt tcaataatct ctggcataat
     1201 ttctcgttgc aattctttat cataaatcga tgtataccag ccaaagtggt ttaaaccata
     1261 atacgtaacg atccaattct tacgatcata accgtaattc actgcaatcg tttcttcaat
     1321 tgaaatagtc atatcacagg cattaatcat ttttacattc ggatatttac gacgaacagc
     1381 ttcagaaacg atcgactctg gatttgtata attcaaaatc catgctttgg gggcatagtc
     1441 ttgtatatga tcgacaattt ccaataaacc tttgattgag cgcatcccat aagcaaatcc
     1501 tccgagtcca cacgtttctt gtcctactaa tccatgttta agtggaatct tttcgtcctt
     1561 ttctcgcatc tccaatcctc caacacgaat ttgtgagaaa acaaaatcgc atcctgtaaa
     1621 agcaagcttt ggatcatccg ttgcacgaat gcgaatattt gaaaaacctt ttcttttcag
     1681 cataaattta acaatcaagt acatatccat atttctttct tcatcaatat catataaacg
     1741 tatttcagat aatggcaacc gtttttcatt gttcaaaatg gcttgaacga ttcctggtgt
     1801 atacgtactc ccgcctccag ctatagtgat aatctgtttt ttcatcatca tcttttcctt
     1861 tctattcttc taataattta ttgaattctg ccataacata agtcacttct aagccgatga
     1921 taatttgata atttttttca gttggtttaa tgatcccgct tatccctaat tgcttgattc
     1981 gctgttcctc gaccatggtg ggatccacta cctctacccg taggcgtgta ttacaactat
     2041 agacatccag gatattggct tttcctccaa acgcttgtag catttttgtt gccatatatt
     2101 cataattttt acttgctaac cctaactctg tttcttctaa ggttacttca ttgccaaact
     2161 ctgccttagc ttcacgtcct aatgtctgaa tgtttctctt attgatcaaa taataaaaag
     2221 taacgtaata caaggcaaaa aagaaaattc cgattggaag aatcaaaata gcattggtag
     2281 cgattcgaaa attgatgaga taatcaacaa tacatgctcc gaatgtaaaa ccaattcgta
     2341 tattgaataa gtaacagaca atccctgcta aaccggcaaa aagtgcatgg atcacgtaca
     2401 acataggaga aagaaacatg aaactaaatt caacaggctc agtgatattt gcaataaatg
     2461 aagttcctgc accgcttccc atgattcctt tgaccatctc tttattttct ttataagctg
     2521 ctttatagat agctagacat atagctggta taccaaacat cattactaca aaaaaaactg
     2581 ataaaaacag tccagcctga ggatctccgt tgataaagcg tgtcatctct cctctgaaaa
     2641 cttccttgct aggagatgta tagcttccta aatcgtaata aatatacgta ttcaacacat
     2701 ggtgcagtcc aaaggggatc agcaaacgat tcaaaaacat aaatatcccc acacctaatg
     2761 ctcccatatc aacgagcagt tttgcaaaat tatcaatccc aatttggatg agcggccaga
     2821 taaagcccaa aaaaacagct gcgattgttt gtagaatcat caccatagtc aatgggaatt
     2881 tttcgcccgc aaaataagaa aaaactactg gtaatttttg ttctttaaag cgattatacg
     2941 tccatgccgc tactaaaccg gaaataattc caccaaaaat ccccatattc acttctgggt
     3001 ccataatttc taagcctttt ttcaaggtag caatcgctaa aaaaccagta agtgcaggta
     3061 ttcctttgtc cttatttttg acaaaaccga ttgcaactcc tatagcaaat aagatatcca
     3121 agtttccaaa aacggcattt ccagctgcac caattactgg gatgttcagc atatcgtcag
     3181 ctgataaacg catgatcaat cccgctaatg gcatcgctgc aactggaagt agcatcgctc
     3241 ttccaagctt ctgcacattt gatttaacat tcatttttat tcctcctcta ttaaatatcc
     3301 tcatatgtta ctaactcaat caaaggatca tttttcactt tttgagcaaa tgctgaatga
     3361 attaaaaatt cgacttggtg ggtacgagga tacacaaaac ttgaaccatt taacaattcc
     3421 tgatccaaat acgctgcgtg acacatgact tcgatcgttt tatcttctct caatatcatg
     3481 agtaaacttg ccaaatattc ttccacactc gtattgattt gtcgtttttt ttcatcagtt
     3541 tctcctacga catcgaaaca aggctcaaaa taatcgacat gacgaacttc atcttttcgt
     3601 tcaaagttgc tccgaaccgg caaatcatat ttttttgcta atgtaacaac tacctcctga
     3661 ttttcatcca aagtatgagt atgatgatga ctgtctaaat gagttggtat gatccccgca
     3721 tgataaattt tttgaatctg agcattccat tcctgataca gttgatcttt gtcaatgtta
     3781 aatggatgct gataataaga taaggatctg aactctccgc tttcgataag agtatcgaca
     3841 gttttgagga gtggtttgcc acacgtcaat gttaaatgga ctcctacacc tagcctaggc
     3901 atttcctttc ttagtttcac agcatgctcg aaacccggca tatttgccat caatgtagtt
     3961 gatgtcaaaa ttcctcgctg ataagcatcc ataatcccat aattcacacc atggctatag
     4021 ccaaaatcat ccgaattaaa aatcactttt cccatttcat tctccttctt ttttctacta
     4081 aaataggcgc aaccaaataa tcatagtcca tttactatgt gtcatttggt tgcgcctata
     4141 gatagttacg cccttatttt aatagattct tgctagatga attgccaaat agatgatttc
     4201 attttcttga atggcaagtt gaaattgctg ttcgatgata tctttaatct ttagtgctgt
     4261 acaatacgct ttgctatgtt taaatgtttc agtaaaaaaa ccaatatttt ctactggcgt
     4321 ttcttcctga taaatcaagc gtttaaccag gaatttgata tgaaccatta agcgatgata
     4381 agtcagtgat tctttatcat aaacgtttga ataggatttt tctagatggg acaaaatcgt
     4441 ggtcgtcatt tctactattt ttaagctctt taaactatta ggctctccta gctcagcatt
     4501 aatgatatgc atagtgatga atcctacttc atttgaccct aactcggttt gatatcgatg
     4561 attgattcgc tcgaccactt ctactgctat atcatattca tttggataaa ggaatctcat
     4621 ttctggcaac ataattgctg tgacatctct atcttctctt tttcgttgaa tagcaaaata
     4681 aatatgatct gtcaatgtaa tatagatatt tgaatctaat ggagaggaga tcctagctgt
     4741 tatcagttca atcacttctt ccgcaaaatc cataaattcg attggaatct gcgagactat
     4801 ctcactgaat cgtttgatct tttctttaga atctaataca aataatttgt cagcatcctc
     4861 aacatcaaat ggcttactca ctttagcaga aaagccaatc cctttcccca tcgcaatttt
     4921 ttctattcca tcgtcatcga tcaatacagc attatgattc agctttttca tcacatacac
     4981 cgttaattca cctcctctat atgataaaac aaaacaactt gcagactcct tcatacatat
     5041 ttttgtctcc aaacaatttt taaagacaaa aaacgaccta agaaagaacc ctttcttagg
     5101 tcgtgcctaa ttgaatagta gcaccctaca taatttacaa aaatagttta acacataata
     5161 aaagcgttat caatatcttt tttactcgtt ttatgataat acttgtatcg aattgatttg
     5221 gaggtaatta cataaaaaca aaaaaatata gcaactctct ataagaaagt tgctatattg
     5281 ataattatac cggcggccgg ggtcgaaccg gcacgtcctc aacggacact ggattttgag
     5341 tccagcgcgt ctgccaattc cgccacgccg gcaaatttaa aaataataac tggggtagct
     5401 ggattcgaac caacgcatga gggagtcaaa gtcccttgcc ttaccgcttg gctatacccc
     5461 aagaataaag ggcgaatgat gggaatcgaa cccacgaatg ccggagccac aatccggtgc
     5521 gttaaccact tcgccacatt cgccatcata aaagatgtta tttaatttct caataaaaaa
     5581 ctaagatgaa gacatcttag tcaataccgg cggccggggt cgaaccggca cgccctcaac
     5641 gggcactgga ttttgagtcc agcgcgtctg ccaattccgc cacgccggca agttgattta
     5701 ttagtactaa ggcggtaacc ggatttgaac cggtgatgaa ggttttgcag acctctgcct
     5761 taccacttgg ctataccgcc attatactat tatcaatcta taaaataaag aataaattat
     5821 ag
//
LOCUS       DAHHZJ010000160         5745 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.162, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000160 DAHHZJ010000000
VERSION     DAHHZJ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.162"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            52..948
                     /locus_tag="NBL02_002421"
     CDS             52..948
                     /locus_tag="NBL02_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HCD2573087.1"
                     /translation="MNKNDVLLIAKNKSLYFFPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAEKYKQEFTKR
                     GVTTNYFILPLRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYQFYHSENIFLLQERDLDELPEFLHTPFQPIPSELLEFFDAENWAKR
                     FFTTDSAIYEQYEYDLSSVKNK"
     gene            complement(1091..2032)
                     /locus_tag="NBL02_002422"
     CDS             complement(1091..2032)
                     /locus_tag="NBL02_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCD2573088.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2158..3279
                     /locus_tag="NBL02_002423"
     CDS             2158..3279
                     /locus_tag="NBL02_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCD2573089.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            3537..5039
                     /gene="eat(A)"
                     /locus_tag="NBL02_002424"
     CDS             3537..5039
                     /gene="eat(A)"
                     /locus_tag="NBL02_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HCD2573090.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            5067..5633
                     /locus_tag="NBL02_002425"
     CDS             5067..5633
                     /locus_tag="NBL02_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCD2573091.1"
                     /translation="MVKENFVNEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQALAEKVVDLPVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELISYE"
BASE COUNT         1874 a         1024 c         1220 g         1627 t
ORIGIN      
        1 tttaaacagg ctatcatagg aaaaagtgac agagaaagtg aggcggaaca gatgaacaaa
       61 aacgacgtat tgctaattgc gaaaaataaa tcactttatt tttttccttc catccgctct
      121 ttagatgttt ggtccggaaa agatttacta cagtcggaat tcattaaatc agctcgacaa
      181 cagttggcaa attatcagct gatcgttttt ttagattatg gatttgatat ggagatggct
      241 cgacgcgtgc gtccatttac caatgcaaaa atcgtgttat ttttttggaa ccactttaaa
      301 gcagaacacg aggcgatgct gaagcaggca caagatgaac cggcaatcga tgagatttat
      361 cattttgatt ttttagaagc gaaagaactt ggactaaaac ataatagttc cttttattcg
      421 aaacatatgg atttttcttc tagcgaacca aagacggatc tattttttgg tgcaacaaat
      481 aacggcagaa aagaacgggc agaaaaatat aagcaggaat ttacaaaaag aggtgttacg
      541 accaattact ttattcttcc gcttcgagga aatgaacaac ctggatacct tacttatgaa
      601 gaatatctga aaaaaaccgg ggaatcgagg gggattctag aactgttgcg ggaagggcag
      661 caaggagtga ctttgcgaac gtttgaatct atgtattttc aaaagaaact agtgacagat
      721 aatcaagcca tagctcatta tcagttttat cattctgaaa atattttttt gcttcaagaa
      781 cgtgatttag atgaattacc ggaatttttg catacgccat ttcaaccaat cccatcagaa
      841 ctgttggaat tttttgatgc agaaaattgg gcaaaacgtt ttttcactac tgactccgct
      901 atttatgaac aatatgaata cgatctttcc tctgttaaaa ataaataaaa gtgccaacca
      961 atcatagatg aaaataaaca agcgcaattc ttatggcaac atgagaattg tgtttttctt
     1021 tttgaacttt catgggggta aaaaaatgag aaccagctgc tgatccagct gattctcatc
     1081 agagagcctt ttattctgcg ccaaggattc cttcttttgc atggactaat tgggtcaaaa
     1141 aggcacaata tggtgtagca atatcatatt tttgtccttt tcgccaaacg gcaccattga
     1201 tgtagtcgat ttcagtcaaa cgatggtttt tgatcaggtc ttggtacata gatgggtaat
     1261 gcaacccgat gttgtcagga tcataagtag aagcaatatg ttggtacact tcttcttgat
     1321 ctaatgtgat tccttctttt gctgcgatag aagcaaactc agatacaatc gttcgaacca
     1381 tgccctctgc cgctttttgc gcacctaatt cagcaatatt acaatccaat atcgtgcata
     1441 atccatttaa tgttccgttt acacaagctt ttcgccaaat ggaataacga acattatgac
     1501 tatagattgg attcaatttt gcttcttgaa agacttctac aacttttttt gtaaatgctt
     1561 cgccttctgg ttccaagttc tctagttcga tttctccatc acctaataat gtgacttttc
     1621 cgggaccagc tagaccagct gtccacatcg tgatcccaaa taagatattt ttctttggta
     1681 catatttttc cagtacatct tcatgaccta atccattcag taaacacaaa acataagtat
     1741 tctcagtaat aatggattgg atcgaacgaa acatatcatc taattgattc gcttttgtca
     1801 aggctacgat caaatccacg tgtttgttgc tttcgatgat ttcttctggt gaatagattg
     1861 gcagttttgc aactacttct ttgccattaa agtcagcaat caatccattt gtacgaatgg
     1921 cttcaatgtg agcaggccag cgatcgatca agacgacctc atttccgctt tgatgcaaca
     1981 ttaatccgat tcggctgccc attgctccgg ctccagctat tgcgattttc atgttttttc
     2041 tctcctttat tcaatgagtt cccttttagt atatagaaaa acagaagaaa atagcattgt
     2101 tttgtttgaa aaaagtgaaa aaaagtgtct tacttaagaa gaaagtgagg gatatagatg
     2161 gatattttgg cggcaatcga ttcatttaaa ggaagtgctt cttcatttga attaaatcag
     2221 gcagttctaa atggttggga agaccgtaaa ggaagaaaaa tcaatgtgcc gattgcagat
     2281 ggcggagaaa atactgcaga agcaatctat tacgcgctcg ggggaacctg gcgtacgatt
     2341 gatgggatgg atctcttatt tcgtcagcga aaaatcagtt atttattgac gaactggcaa
     2401 ggaaaaaata ccgcagtcat tgaatctgca caagtattag gattagattt acttgataaa
     2461 ccaacggatc aaaccatccg acttgcttct agttatgcct tgggagagct tatcaaagat
     2521 acactgcttc aagaagtgga acaaatcatt gtcactttag gaggaagcgg atgtagcgat
     2581 ggtggtctag gattactcca atctttgggt gcttctttag aagggtacac agaaggaaat
     2641 cctttgttat ctactagaaa acttcatttg gatcgaagga acgttcttgt ttctaaagta
     2701 aaagtgatca tcgctgcaga cgtgaccaac ccatatacag gtcaacaagg agctgcttgg
     2761 caatttggaa gacaaaaagg aggaacggat acgacgttag ctttcttgga taaacaagct
     2821 gaaaagattg ctcagcagat aaaagatgtt tgggaggtgg atttagatga catcccagga
     2881 agcggagcag caggcggtat tggcggagcc ctttttttgt taggagcgaa gatggtctct
     2941 ggttttgagt tggtcagcca aatcactgga ttagaaaaac agatcgaaca agcggatgtc
     3001 atcattacag gagaagggcg gattgatcag caaacaatcc acggaaaagt tccatatggc
     3061 gtagcagtgc ttgcaaaaaa atatcgaaaa ccaataatcg ctatttgcgg aagtagagac
     3121 cgagagctgg aaggactctt tgattttctc cccattgtct taagcattca attaggacca
     3181 atcgatttag aggaagcaat ggaacacagg caaacattag aaaaaacaga aatcttagga
     3241 cagacactca gcagattttt caacattctc ccgaagtagt tgacaaaaac aatttcttta
     3301 tgctatcgta atttcaaata taggacaaag tgagggatcc gtgcattgaa aaattaagtc
     3361 gattgatgaa agctgcattc agactgtacc ttgtggagac aagggagcag tgtgcagatt
     3421 ttcgggattg gcttggttct ttcgatgtat gggtcttttt gtgtgcccca acccttgcat
     3481 cctctttagc cagttctgaa aataggactg gtcttttttt acgaggtgat ggaataatgt
     3541 ctaaaatcga aataaaaaat ctgacattcg gctatgacag ccaaggcaca ttattatttg
     3601 aacaagccaa tctaaacttt gacacacaat ggaaactagg acttatcgga cgaaacggtc
     3661 gaggaaagac aactttactg aatatcctgc aaaacaaact cccttatcaa gggcaagtga
     3721 tccatcagca agcgtttgtc tatttcccgc agcaaacaaa agataaagag cgtttgacct
     3781 attatgtgtt aaatgagatt atggattttg agatatggga aattgaaaga gagctccaac
     3841 tgatgcaaac agatcctgaa atcttatgga gagaattcag cacactatca ggaggagaga
     3901 agacaaaagt cctactggca ctcttatttg tggatgacac tcatttcccg ttaatcgatg
     3961 aaccaacgaa tcatttggat atctctggca gaaaacaagt agcggcttac ttgaaaaaga
     4021 aaaaacaagg attcatcgtg gtgagtcatg atcggggatt tatcgatgaa gtagtggacc
     4081 atgttttagc aatcgaaaag agccaactgg aactttatca agggaatttc tctatctatg
     4141 aagaacagaa aaaacttcgt gatgaatttg aaatagctaa aaatgaaaaa ttgaaaaaag
     4201 aagtcagcag gctaaagaaa acagcagctg aaaaagcaga atggtctcgt tcccgagaag
     4261 gggacaaaac aaagaaaaaa gtcggattca tcgatacgga atctaggcga gtgaataaag
     4321 gagcagtagg cgctgatgct gcacggacga tgaaacgatc taaagcaatc gtgaatcgga
     4381 tggaaaccca gatcagtgag aaagaaaaat tattaaaaga tatcgagtat atcgatgctt
     4441 tgacgatgaa tagccaagtg tctcatcata aacggctgtt aagcgtagaa aatcttcaat
     4501 tagggtatga aaatctgtta ttcgagccag ttcattttac gatcgaacct catcagcgga
     4561 tggcaatttc aggtcctaac ggcgcgggaa agtcatccat tatccattat cttctgggga
     4621 cattcaacgg caaggttgta ggagaaaaat cccagcccaa acatttgacc attagttacg
     4681 tacgccaaaa ttatgaagac aatcgaggga cattggcgga attcgcgaag aaaaaccgag
     4741 tagactatca agcatttttg aataatctcc gaaagcttgg gatggaaaga gatgtttttc
     4801 ataataaaat cgaacagatg agtatgggac aacggaaaaa agtggaattg gctaaatctt
     4861 tatcccaacc agctgagctc tatatatggg atgaaccatt gaattatttg gatgtcttca
     4921 atcaagaaca attagaacaa ctgattttga acgtgaaacc tgctatgtta ctagtggaac
     4981 atgatcaaac ctttctagat aaagtatcta ctgagattat ttctcttgag aaaatctaaa
     5041 ccttgtaatc tcaaagacaa caaggtatgg ttaaggagaa ctttgtcaac gaaggagaga
     5101 atcacatgct ttatgtaacg agacacggag aaacaacttg gaacgcacaa ggattagttt
     5161 gcgggcgagc agatgtgccg ctgactgaaa aaggacagat gcaggcacag gcactagcag
     5221 aaaaggtagt agatttgcct gttccaatca cgaaaatcat ccattcccct cttcaacgag
     5281 caagagatac agcacaagca gtagttgatc gtctcaattt gccgatgacc gctgatgagc
     5341 gattggtaga aatggatttt ggggattatg acggactgcc aagcaaagat gaaaactttc
     5401 aaaaagcgcg tttagcgttt gctgtacgtt tcccaaatgg agagtcggtc ttagatgtgt
     5461 acgcacggat cgttccttta ttgaaagaat gcatggaaga cgaagaaaat gtctacttgt
     5521 tagtctgtca taatgccttg ataagagtga tcaatgctta ttttcatcca atgcctaatg
     5581 aagggttctt tacattcatg gtagacaata cagaattaat ttcttatgag taattagact
     5641 tttgtgttat ttaaataaaa tgtttcaagg cactgctttt gcttagcagt gccttttttg
     5701 tggctgtgtc aattcttttg gcattataaa tccaattttt agaaa
//
LOCUS       DAHHZJ010000161         5648 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.163, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000161 DAHHZJ010000000
VERSION     DAHHZJ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.163"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(205..882)
                     /gene="nth"
                     /locus_tag="NBL02_002426"
     CDS             complement(205..882)
                     /gene="nth"
                     /locus_tag="NBL02_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCD2573092.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(889..1587)
                     /locus_tag="NBL02_002427"
     CDS             complement(889..1587)
                     /locus_tag="NBL02_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HCD2573093.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1855..3039
                     /locus_tag="NBL02_002428"
     CDS             1855..3039
                     /locus_tag="NBL02_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HCD2573094.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3045..3488)
                     /locus_tag="NBL02_002429"
     CDS             complement(3045..3488)
                     /locus_tag="NBL02_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCD2573095.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3714..4001)
                     /locus_tag="NBL02_002430"
     CDS             complement(3714..4001)
                     /locus_tag="NBL02_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573096.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4172..5524)
                     /locus_tag="NBL02_002431"
     CDS             complement(4172..5524)
                     /locus_tag="NBL02_002431"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HCD2573097.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
BASE COUNT         1486 a         1131 c          959 g         2072 t
ORIGIN      
        1 attggaaggc attattttct acatcgggta ttttcccaat aaaaataaca acttttattt
       61 ttatcgcaat aaattgtaat aagttaccat cctagctact gagtatgtac atttactttt
      121 tcatgcaaaa aagataaacg aaacagcttt tctccataat aacaagataa ttcatacatt
      181 tatttgcaat taaagaaacc acgtctacag gcgtggtttc ttttttagcg ccttttcttt
      241 tagacgcatc cgatttttcc catcttgaca tattgatagc aaaggacata cctcgcactt
      301 gggatttctt gccgtgcaat gatatctacc aaaaaaaatc agtgtatgat gtgtctttac
      361 ccataattct tgtggtactt tccgcatcag tgtttcttct acttccatga ccgtagcatc
      421 tagtttgcag atcctcaggc gttttgagac acgttccacg tgggtatcta ctgcgattgc
      481 tggtatcccg aatgcatccc ccaatacgac atttgctgtc tttctgccta caccaggaag
      541 agacatcaat tcttctcttg acgttggcac ttgcccatcg aaacgttcaa tcagctgctg
      601 tgcacacgct ttgatgtttt ttgctttatt tcgatacaaa ccaatcgttt ttattttaag
      661 aatgatttca tcgattgaag cttctgctaa cgcatcgggt gtcggaaaag aggcaaacaa
      721 atctggcgtt gctttattca ctgatacatc tgtcgcctgg gcacttaaga tcactgcaat
      781 caataactca aatggattat tgtgcttgag ttctccatgt gcttcgggaa acattccata
      841 catcgtttct agagcttcca ttgttttttg tttgcttaac atattccgct actcctccaa
      901 ccaatttttc agtgagactt tcggcaattc ttcttgttga gctgactgtt ctacttctct
      961 ttgaagaagc tgctgttttc ttcgtttttg gtcttcttcc acttgttgtt ttgtacgtag
     1021 attttttctt tcccacgata gcaaaatgcg gtcaacgtaa ttaaaactat aagcctgatt
     1081 taatacggct tcccgcagtg ctagcttcaa aatttccggc tgataatggt cttcttctag
     1141 ccattgtccg atccgctgga actcgatcga agaaagcggg cgtccaaatt cttgttcaaa
     1201 taattgatac actgactgga tttctttttc ttgtgttttt tcttcgtatt ttttttcttg
     1261 tgtttttaaa tactcaccta atagatcata gatggggtaa agattatatc gatctgcttt
     1321 tttaccgttg acaatcgtcg tttctatctt gataaatcca tttgttacga gacgatccag
     1381 tatttgaaaa atctgatctt gtttgattcc catatccata gcaatcatct gcaaatcagg
     1441 gaaagtatct ccttcaagct gtgccatatg caactgtaga acaaataaaa attcttcaga
     1501 ttgtagaccg atcctatgat aatttttaag taaaagatta gatatgactg tttgtccagc
     1561 gcttaagtaa ttttctaaat tcaacatgat ttctcctccg ctcaatcaca gtatacgtaa
     1621 aaaaaacatg ggaagcaaga acaggtttct tttctattta cttcactgat cggtcagatt
     1681 tacaatgtcg tctacttgat ttttgtttgt tcatgttttt gatgcgtatc ttcacttttc
     1741 ggaataaata aattaagtag atttcgctgt tccttagaat gttgacaagg aaacaaaatt
     1801 actatacact atttttgttt ccaagttaac attattgcat aaaggagttt ttacatgtac
     1861 agtttcacaa ataaacattc taaagttttt ggatacgtct ctgtcttatt cactggatta
     1921 ggatttacta tcgtcagtcc tgtacttccg tttttaacgc tgccttattc tcactccata
     1981 catcagcaag ctttctatat cactttgctc atgtctgttt atgctctagc tgccttctta
     2041 tctgccccca ttctcggtag cttaagtgat cattttgggc gtcatccgat ccttattatc
     2101 agtttattag gatcaagtat cggatatttg ctttttggtt taggaaattc gatatggatg
     2161 ttgttccttg gtcgtatcat tgaaggattg acggcaggag aaatctccac cttatatgct
     2221 tactttgccg acatcacgga acctaatgaa agaaccaaag tttttggatg gatgggtgct
     2281 ttagtcggta taggaacaac gctgggtcct attatagggg gtctattagc tgagttgggg
     2341 aattctgttc ctatatttat tggagcactc tttactttct taaatgccgt ttatggctac
     2401 acatttatgc tagaaagcct tccaataaaa aaacgttcag tagatttaag tttctcccat
     2461 gtaagacctt ttcaccaatt acaacaatta ttcaagattt catccgttat tcctttattg
     2521 acagcaggtt ttgctgtttg gttggcagct ggctctcttc aatctatctt ttctcaattt
     2581 tcgattgacg cctttcagtg gaaggctggg ttggttggtt taagcttctc tttgattgga
     2641 atcttggata ctgtctcgca attgttcatt atgcctcgat tgctcaaaaa attttcagaa
     2701 cagcagatca caagaatagg gatgttcagt gaaatcctcg cctatctatt catcacatta
     2761 tctggaattc ttctactgcc gattttgttt ttatttggga ttatctgtta tggtttcggt
     2821 gattctattt ttactcctgt attcaatggc caactatcca attctgtctc agataaccag
     2881 caaggtcttg taatgggcgg tacccaaagt atacaatctc tatcgcgcgt catcggcccg
     2941 ctaatcgcag gacagcttta tgctgcagca ccatttcttc cgacggtatt cggatttttg
     3001 cttatgattt ttgcctatta tcactacaat aaaaaatagt agcttcattt gctacttcgt
     3061 ataaattctc tgtatcgacc gataaaacga aatactgtgt caaattcttc ttttgacata
     3121 ttttcaaata cagtagcatc tcgctgtacc catttttgat ggagttgctc gtgtctattc
     3181 cttatttttt gtccgattgg cgtcaattga aaatagattt cttttttatt ctcttcgctt
     3241 tgataagtgt ttatgatccc tctagctatc agtttttttg ttagcttact gatggctcct
     3301 ctagtcatgt aactagcatt cgctaatttt gtcacgttag gatcagggat tttcccgata
     3361 tgttcaatca cttctagttc atttagagta aatcctgcaa attctttttc catttctggt
     3421 ttattacgat gaataatctc attagctaaa tccagatatt cttctattag cagttgtctt
     3481 ttatccattt ctacaccttc tttttctttc attatattgt ttttttgttt cctattcaac
     3541 aaagataaaa atattttgta ggaaaaccca atctgaaacc aagaaaaaaa ggggttgaca
     3601 aagcttgtca taaccccttt ttctgattaa aagatagtaa agagacagtc atcggtaatt
     3661 gtgatgattt tttgtcatca cttaaggatg agcggccttt tcaaaaactg tctttaaaga
     3721 tcatcatcag tttctagcac atatggctga ccttcatatg tgtactctgt attcaatgtg
     3781 ccgtcatttt catctgagta gatgcgtcta ctgaaaggaa aacgatgaga aacattatct
     3841 ttggcgtcat ttgctggcat cgtgtaatat tcctcttgcg tttcattcaa ctgagagagt
     3901 acttgatctg ccaactcatc aatattgatc gttaatttct tcgaagcact tttataatct
     3961 tctaaaaatg catcttgttg gacaaaatat tctttattca tcttctttct cacctttctt
     4021 ttcaaaatca aataaagctg cttgttcttt tatgtaacag ctttatcttt gctgcttatt
     4081 gtgacaaaaa attcgaagaa agacaactaa ttgtactttt cacaaaaaaa gacagagggt
     4141 cgtgacaaaa ttgtgtcaca accctttttc tttattgtgc agtttcgatt tcgcctagcg
     4201 ctgcttgtgc aagtaggttc aagtaattcc atgggcggtc aaaatgtggt tggaagaaga
     4261 aatctgaaag tgcgagatct tctacagtca ttttattttg aacagctaaa gacatggtat
     4321 ttgctgattg tgtaatgtcg tatttggaca tgaactgagc acctacgata cgattcgttc
     4381 ctttttcata gactagctcc atcattactt tttcagtagt tggcatgaac tctggacgat
     4441 aattatcttc aaatacagtc gcttggactt ctagatcgtt caaagttgcg ctttctttgg
     4501 tcacacctgt cgaaccgatc gtccagccaa ataggtaaag tcctgaagtt ccttgagtac
     4561 cacgatacgc cattttatga tctgtcaagt tatttcccac aagcaaacct tgacgaacgg
     4621 catttgttgc caaaggaata tagttttgcg tgccacttgg attgtagttg actactgcag
     4681 agtctccagc tgcaaaaatg tctggattac ttgtctgcat gtattcattc actttgattg
     4741 ccccgtttgg cagtgtatct acttttcctt cgatcagttt tgtatttgga cggaagccca
     4801 cacataaaat gaccatatct gtatcaaatg tatctgttgg tgtagctact tgtttcactt
     4861 ttccttcttc atctgtgata aattcagtaa cattttcgcc tagcgcaaga gtaacaccgc
     4921 ggtctgtcaa ttctttttct aagatatctg tgaatggttt atctagatat ttattcaaga
     4981 tgcgatctaa tccatcaatc aatgtcactt gttttccaga ttcgacaaaa gcttctacta
     5041 attcaatacc gatatatcca ccaccaacaa ttactacacg ttttgcttct ttcgcttgtg
     5101 caataatttc atttgcttga ttgtagtttt tgcataggag tacatttttc gcatcgattc
     5161 cactaatcgg tgggataata ggccatgaac cagttgtcat cactaattta tcataagaaa
     5221 ccgtttctgt ttctcctgtt ttcagatctt ttgccgttac tgttttattt tctgtatcga
     5281 cgtctgttac atcgtgttcc attttaactg ttgctcctaa tgaagataat tcttcaggat
     5341 ttgagtagaa caagcctgct gggtccttta ctactccgcc gacataaagt gcgataccgc
     5401 aagataagaa agaaatattg tcatttcttt catatactgt cacttctgct tctggatgat
     5461 tttttaaaat actttttaca gctgcagtac ctgcatgagt acagccaatt actactactt
     5521 tcatttatgg tccctccgat taacattttg tccttcgaca aaactactat aacagaaata
     5581 aaacggatac aacagtttaa tgcttgtgaa ttcacaaagg tttgtttgta gtttcattcc
     5641 ttttcaag
//
LOCUS       DAHHZJ010000162         5543 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.164, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000162 DAHHZJ010000000
VERSION     DAHHZJ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.164"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            66..767
                     /locus_tag="NBL02_002432"
     CDS             66..767
                     /locus_tag="NBL02_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HCD2573098.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            764..1885
                     /locus_tag="NBL02_002433"
     CDS             764..1885
                     /locus_tag="NBL02_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HCD2573099.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1900..3132
                     /gene="pepT"
                     /locus_tag="NBL02_002434"
     CDS             1900..3132
                     /gene="pepT"
                     /locus_tag="NBL02_002434"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCD2573100.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3264..4172
                     /locus_tag="NBL02_002435"
     CDS             3264..4172
                     /locus_tag="NBL02_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HCD2573101.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4207..4479
                     /locus_tag="NBL02_002436"
     CDS             4207..4479
                     /locus_tag="NBL02_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HCD2573102.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4490..5536
                     /locus_tag="NBL02_002437"
     CDS             4490..5536
                     /locus_tag="NBL02_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HCD2573103.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         1796 a          964 c         1209 g         1574 t
ORIGIN      
        1 ttttttattc ctagtctcct tattttattt ggaaaatgct ataattatct agaaggagtg
       61 aagctatgaa ccatttagat ttatctcagc gcttagccgc agtgggtgac tttgtccctg
      121 aatccgctcg tttagcagac attggatcag atcacgctta cttacccgtt gcattgatat
      181 taagaggaaa aatcgaatat gcagttgctg gtgaagtcgt tcaaggtcct tatgaatcag
      241 cacgtaagca agtcaaaaaa gatggattag aagaccgtat caccgtacgc cttgcaaatg
      301 gtctagatgc tatccagcca caagatggta ttacagctgt cacgattgcg ggtatgggag
      361 gttcactgat ccgagatatc ctagaagctg gcaagttgaa tcaacgctta agcggacaag
      421 aacgtttgat ccttcagcca aacatcggag agcgtacatt acgatcttgg ctgcaagaaa
      481 atcagtataa aatccaatca gaaaaaatac ttgaagaaaa taaaaaaata tatgaaatca
      541 ttgtagccga aaaagaacaa aaaccagtta cttacaatga aatggagctg atgttcgggc
      601 catttctgct tgaagagaaa agtgagatct tcaaatcaaa atggacaaga gagcttcaac
      661 aaagaaaagc tatccgtgaa cagctcaaac aagcagctaa tcaagaggca cgtcaaaaac
      721 aaatcgagca ggaaatccaa atgattgagg aggttttaaa gccatgacaa tcagcggacg
      781 cactttcatc caaaagtttg aaagctattg tcctcagtgg ctagctgaag aaggagaccc
      841 agtcggactg catatcggaa cattggataa gccaatccaa cgagtaatga tgacattaga
      901 cgttcgtcca gaagtggtcg aagaagcaat caagaaaaaa atcgatttgc tgattgctaa
      961 acatcctcca atctttcgtc ctgtaaaacg gctggttact gatcagccac aagaaaaaat
     1021 gtatgcagat ttgttgaaac atgatattgc cgtctatgca gcacacacga acatggatat
     1081 tatttgggat ggactgaatg actggttttg tgaacttctc ggtattgaag tggagagtta
     1141 tttagtaaag actcatgaga ttcattataa aaaactggcc gtttatgtac ctgtggatca
     1201 tgcacagaaa atgcgtgaag tattagctgc agctggtgct ggtacacaag gtgattatac
     1261 cggtacaagt tttacctcga ttggacacgg tcgatttact ccggaagctg gtgcacaacc
     1321 agcgattggg aaagtaggga agacagaaca agttcaagag gcaaaagttg aggttatttt
     1381 gccggaaact atcgaaaaac aagtgattca agccatgcgg tcagctcatc cttatgagga
     1441 acctgcttat gatcttttcg ctatagatga accagtcgaa atgtttgggt taggacgagt
     1501 aggtgaattg ccgcaagaaa tttctataga ggcatttgta gaacaagtaa aagaagcgtt
     1561 ccaactagat ggactgagaa tagtccagcc aaaaaatgca aaatcctctg tgaagcgcat
     1621 tgcgatttgc ggtggaagcg gtgaaaaatt ttatccgcaa gcaatcgccc aaagagcaga
     1681 tgtctatatc acgggagata tctattatca tacggctcat gatatgcaaa gcgctgggtt
     1741 gattgcaatt gatcccggtc attatattga atcgttatgc aagcaacgat ttatcgagaa
     1801 atttgaatct tggaaacaag aagaaaattg ggatattaac tttttcgttt ctgaaacaaa
     1861 tacaaaccca tttcaattta attaaaagga gagaatacta tgtacgaaaa tcttttgcca
     1921 cgttttttac gatatgtcaa aacagaaaca cgctccgatg caacaagcaa aacgactcca
     1981 tcgactcaga cacaagttgc ttttgcgcaa gaattgaaaa aagaattaga agagttaggc
     2041 ttagctgatg ttttctataa tgaagataac ggattcgtta tgggaacatt accaagcaat
     2101 gttgatcatg aagtgcgttc cattggattt atttcccaca tggatacagc agattttaat
     2161 gcagtaggag tcaatccaca aatcattgaa aactatgatg gcgagtctac gatcgtacta
     2221 gatgcggaag aacgctatac attgaataca aaagattttc caaatttgaa aaattatgaa
     2281 ggccacacat tgatcacaac agatggcagt acattactgg gagcagatga caaatcaggg
     2341 attgcggaaa tcatgactgc aatggagatc ttgatcaata atccttctat ccctcacggt
     2401 accatcaaag tggcttttgg tcctgatgaa gaaatcggtg tcggagcgga taaattcgat
     2461 gttgagcaat tcgatgtaga ttttgcttat acagtcgatg gtggtccttt aggtgagttg
     2521 cagtatgaaa cgtttagtgc tgctcaagca aatattacga tccaagggaa aaatgtacat
     2581 ccagggacgg caaaagatac aatgatcaat gctctacagt tagcaatcga ttttcataat
     2641 cagcttccag ccgatgaagt tccagaaaaa acggaaggat acgaaggttt cttccatttg
     2701 atggcattgg atgggaatcc tgaagaagct tctatgagtt atatcattcg cgatcatgat
     2761 cgtgaaaaat tcgaagcacg caaagcacaa atcacacata tccaagaaga gttgaatcag
     2821 cgatttgatc aagaacgaat caaagtagat atgtatgatc aatattacaa catgaaagaa
     2881 atcattgaaa aagatatgag catcgtggaa ttagcgaaac aagcaatgat cgagcttggt
     2941 atctctccag tgatcgaacc agttcgtgga ggaacagatg gttcaaaaat ctcttacatg
     3001 ggtattccga cgccaaatct atttgccgga ggagaaaata tgcacggacg attcgaattt
     3061 gtttctttac aagtaatgga aaaagcaaca gacgtgattg taaaaattgc agaattgaac
     3121 acaaagagtt aaaatcgaaa aagaacagct tatgacaagg cgttcgtccc aatgatggaa
     3181 tgtcatctgt ctaaggctaa agggcaggac aatggaattg gttgtcccgc cctctttttt
     3241 gaagagtaaa taggaggtaa agaatggaaa agctgctagt tttttataac aaaagttcag
     3301 ggaaagatga gggtgagaaa ttagcacgct ggtttcaaga ttatgtaaaa gaaaagcagc
     3361 cagaattata tgtagaacta gctgaaactg gcccatcgat tgatgatgcg gaattagtca
     3421 agaaagcaga agccatagaa gctgatactt tagtaattat aggtggagac ggcacgattc
     3481 atcatatcgt ccaagcgttc caaaaaacat tatctcaata ccatgtcgga ttacttccag
     3541 gtggaactgt caacaacctt gcacgtgttc ttgagattcc cctcaaaaaa gaagaagctg
     3601 ctgacgtgat cctggaaggc aatgtacgga aaatagacta tggtcaagtc aatgatgaag
     3661 tcattatctc tacactgaca atcggaatct tagcagatac agcagtttgg atcagtcaag
     3721 aagaaaaaca gcgttacggc tcgtggatct ttatcaagaa gttttttaag ttattgctga
     3781 agaagaaaaa atatcatttg gatttagaaa cagacgagga gcattggaaa ggaaaatccc
     3841 aactgttgac agtcacgatg tctaactccg tgggcggttt tacaaatttt gacgactcag
     3901 cgaccctaga tgatggaaaa tttcatgtaa cgatcgtacc ttccttagat attttccgat
     3961 ttatttttta tttgcctaga atcatgagag gcaaattcta tacgatcccc ggcattactt
     4021 atttcacagc caaacgcatg aaaatccaag cgcaggaaaa atctgtgggc actcgaacgg
     4081 atggagacac gacagatgat cttccagttg aattatctgt tgtatcaaaa ggattaacag
     4141 tttattcacc aaagaaagaa gaaaaagctt agaaatagta gttaacagaa agttggaaga
     4201 agagtaatgg atgtattgtt acacaatcct ttgtggtttt accttttcat tgtgaatttt
     4261 tacttatttt gtctgatggg gtatgataaa catcaggcta gaactagcgg ctggcgtgtt
     4321 cctgagaata atttgatatt tgtcagcctg ttaggcggtg gtttgggagg attgatctct
     4381 cgaaaagttt tccatcataa aactcggaaa aagaagttta ctataggatt cgctgcaggt
     4441 gtacttgttg atttgattct catttatttt ttccattaag gagcaggaga tgtcgtctaa
     4501 aggaacgaaa actaaaatca ttctgaatct gcttttgctg attgtgattt ttgccattat
     4561 ttattattta atcaatcaat catttgcaga tatctttgcc gaattgctgt ctaccagtgt
     4621 tgttgttttg gcagcggctg ttatattagg gacagtttac caaatcgctg aagggcgctc
     4681 aatcaaagaa attgctgaac ctttcgcgcc gaattttaca acgaaagatg ggttcttgac
     4741 ctctagctat attgcgtttt accgaatcgt ttcgtttggt acaggaacgc tgatctcgga
     4801 gatttatttt tataacaaaa aaggaatgaa gctatcccag ggagcagggg tgacagcact
     4861 ccatatgatt atgtacaaac tggcagtgat cacttatgca ttagctggct tggtcatcca
     4921 attctctctt ttttattcta atggtccatc aatgatctgg tttatcatcg ctggtatcct
     4981 tttgacgatc gtgattgttg cctttttatt ggcgatgtcc gtcagcttga atgttcaaat
     5041 attttttgtc agcttatcga ataagttatt taaaagtgag cgtttgcgga aatgggtgga
     5101 tacatgcaat cagcagatct actctttaag ggaaacggtc caaaccatta tccaagacag
     5161 gactgcgcta ttgcggattt atctatggaa tatgttaaaa cttgtatttt ggtatgtact
     5221 tccttatttg attctgattg aaaaccatcc taaaatagat tttctactga ccatttcgtt
     5281 tatcagcttt gcagtagtgc tttcaggggt tattcctacg cctgctggta ttggttcctt
     5341 tgaatttgtc tatttgttat tattcagacc agttgttgga acagttgacg cagtttcttc
     5401 gcttttactg tatcgtttca gttcctttat cttgccattt ctctatggat ttgtgtatgt
     5461 gattgctgaa cggcgaagag tgatcaaaga agaaataata gaagtaaaaa aagagaataa
     5521 agaaaatcaa gggtaggtct ttt
//
LOCUS       DAHHZJ010000163         5540 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.165, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000163 DAHHZJ010000000
VERSION     DAHHZJ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.165"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            107..1248
                     /locus_tag="NBL02_002438"
     CDS             join(107..335,335..1248)
                     /locus_tag="NBL02_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCD2573104.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            1483..1935
                     /locus_tag="NBL02_002439"
     CDS             1483..1935
                     /locus_tag="NBL02_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573105.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            1949..2638
                     /locus_tag="NBL02_002440"
     CDS             1949..2638
                     /locus_tag="NBL02_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573106.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            2763..5114
                     /locus_tag="NBL02_002441"
     CDS             2763..5114
                     /locus_tag="NBL02_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HCD2573107.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            5194..>5540
                     /locus_tag="NBL02_002442"
     CDS             5194..>5540
                     /locus_tag="NBL02_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573108.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSK"
BASE COUNT         1919 a          826 c         1116 g         1679 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc gtagaatcta actactaaga aaaggacgtg tccacgatgg caaaagccaa
      121 gaaaaaattt gatgaagact ttaagaagat gatcttagat ttaaaccagt ctggccaatc
      181 ggtcgaagaa ctggcagcgc aatatggtat cgctacacaa acgatttatc gttggaagaa
      241 gcttcataca aagaatgtag caaccggcat gactgaggca gaaatcctag cgatgaaaaa
      301 ggaaatggcc cgcatgcagg aggaaaacac tatcctaaaa aaggctttaa ccatattcgc
      361 tcaaaagtaa aaatgagtga tgtcttcgaa tttattcaat caaatgttca tgatcatgac
      421 gtaaaacaaa tgtgtaccgt tttggaagca ccgaaatcca gctactatga ctggaagaaa
      481 aagaaacctt ccaagcgcca atcagaaaac aaacgcttaa accgcttgat ttcaggaatc
      541 tactttgaaa acaagggtat ctatggtgcg cctaaaattc ataagattct tgttggccgt
      601 ggcgagacct tgtccttaaa gaaagtacaa atactgatga gaaaattagg cttgcgttca
      661 gtcaccctca agaagtacaa gcctggcaaa caagcgaaag ttaaaagtga aggacgtaaa
      721 aacctgttaa accaagattt taccactacg tcgatcaatc aaaaatgggt gacagatatt
      781 acctatattc ataccctaaa agatggctgg acctacctct ccactattca agacctccac
      841 acaaagaaag tcatcggttg gaagtttggc aagcagatga cgaaagaatt ggttatcgaa
      901 acccttgaca atgcgctctt aaaccaaaca ccctccgaga acttgatcat ccactcagat
      961 ttgggttctc agtacactag cgaagcctat gaggcaaagc tgaatgaatt gaacattcgg
     1021 cactccttca gtcgtaaggg ctgtccctac gataatgccg gtatcgaatc gtttcacgcc
     1081 tcactcaaaa aagaagaagt ctatccatca aaagcgtatg aaaactttga agctgcttat
     1141 ttagcgttat ttcaatacat tgaaggattt tacaatcgca agcgaatcca tagtagtatc
     1201 aactatttaa caccaaatca gatggaggag ttggcattgc aagcttagtt cctagctacc
     1261 acttacattt ttttggcgag atttccacag ggtttcaact tcatcgcttc gatcagcggt
     1321 caaacggttt tggtttgacc gctggtcgaa acgatgagat actcacccgc tggaatctcg
     1381 aaaaaattct cacaaatcgt gtccgaggta ttgactcaaa tccaccaatt atcttatcgt
     1441 agatatatat tataaattta ataagaaagc gaggtgagaa tcgtgaaaaa aacgatacta
     1501 agtatttttt cagtatttac aatattaatt gggggctatt ttcttttgaa tttagattct
     1561 gttaattcaa acgctcaaat aaaagatcaa agagtagctg aacataataa taaaaaaatg
     1621 agtttgacca aaaatatcta ttctatgtca gatgaagaat tgtataaaga atttggtgat
     1681 ttatccgggg aacagttaga agaaagtatt ccaggaatta gtgactcaga taaaattgtt
     1741 gtcttgtctg ctggtgggca attaatggga gatggcaaat ccgaattagt tagttataaa
     1801 caaaatcaca gacagtttat tgatgttaat actgattcca acgctatttc gattaaggat
     1861 ctaaaagagc ttttaaatag taaaagaaat gaattaaagc agataaaaga aactaattca
     1921 gtggaattag agtaaaggaa gattcattat gaaaaagatt tttggtgttt ctgttgggct
     1981 acttatgatg ttaacatttg gaaatgcagt atatgctgct gagaaagaag ttatttcaga
     2041 tccagagcag tacgcctcac gatactttgc tgattttgaa gaagaagata aagtacttgc
     2101 attgccagaa ggtggttttt taaaagggga agctaaaatt gtatctatgg ataatccaga
     2161 tataattcaa ggttcatata attcagaaac tgatcctgct tcagtttcag tgacagaagc
     2221 aaaaaaaatt gttgctgaaa aattcaaaaa tgatattgaa actactccaa ttatgcctcg
     2281 aggagcattt ccacccgatg ttggtactag cggacacctt cttgtactag caggaggagg
     2341 aagctataaa tcacagccat ttagtggtgc gggatggcgt tttggaggta tgggtgtaaa
     2401 accagctgat ggtacaggca actttttacg ttggaaatcc tacattgacg atggaagagt
     2461 aggaaatgta aatcaagcca acgcaactaa atctggttct attcaaggaa tggcattgta
     2521 tgctcgtgaa ggattcaaat actttgcacc aaacgggcct ggttggacaa tgaatatagg
     2581 tatttattac acttataatc ctgttgcagg tacgtattat gttgttcaaa atatttaata
     2641 aaacagcaca aagagatttc tttgtgctgt tttattattt ttagttgttg acaaacacta
     2701 aaatataaaa tataatcaac acataaacga acgtatgttc ggcaagaaag caggggattt
     2761 ttatggatga gatggtttta agtactcaga aatggctaaa caagaaatac agtaatgtta
     2821 cagggtttga taaggttcca gaaaatgggc gaacagggtg gcctacaatc tatggactta
     2881 ttgaaggact acaagtagaa cttggtatta ctaatctagt agctaatttt ggacctacca
     2941 ctgaaaaaat gtacgataat caggttactc ctaaatgggg caaaaatcta cctaaaaata
     3001 ttgtatttct tattcaaggt gccttttggt gtaaggggat taatccaggt ggcttcgatg
     3061 gggtatatac tccatattta gatactgctg taaaagagtt gcagactgat gcagggttta
     3121 atggaagcgc tgtaactgga gttttggatg cgaaatgggc caaagcgtta tttgatatga
     3181 gtgcttttgt tcttgttccc ggcggagata gtaaaattag aaaaatgcag caatggttaa
     3241 atgtaaatta tttagaatac acgggaataa tgccttgtga tggtatttat caaagaaatt
     3301 caaatcaagc attaattttt gcattacaag ctgaactagg atattctccc agtgaagcaa
     3361 cagggtctta tggtaatggg acaactagta aaacttatcc tgtgtctgaa ggtaactcgg
     3421 gaaactatgt aagaataatt caatatggat tatatgttaa tggttatttt gaagacggta
     3481 cattcgatgg tattttcact aaatatatgg gtttagaagt actggcattc cgaaaattta
     3541 tggtattgcc tgaatatact gaaatagcag atgttgttgt aataaaagga ctattgtcta
     3601 gcgctggaga tattagaaga tctgcagatg gcgcagatac ttctacgcaa ttaactagat
     3661 ctcaaatcca aacattggtt gataatgata taaaaatagt tggtagatat cttactggta
     3721 ctgttggaat tggaaaagat gaacgaaata agtatctaac tacagaggaa ttgaataata
     3781 ttttttctaa aaatttatct gtgtttccta tctatcaaga tggaggtgca gcattagctt
     3841 attttactta tgatcgtggc ttatcagatg gtaagaaggc tatagatgca gcaaaaaatc
     3901 taaatatacc attaacaaca gttatttatt ttgctgttga tctagacatg ttaggggaag
     3961 aaattcttgc atatgctgga gaatatttta agggggtatc tgctgtaatg tcatatagcg
     4021 ggtatcaaac gggtgtttac ggaacaagaa atgtttgttc tcaaataatc aataacgggt
     4081 atgcttcgtt tgcatttgtt tcagatatgt cgactggtta tagtgggaat ttaggttttc
     4141 ctatgcctag acaatggtct tttgatcaat ttgttgaatt taccatagga agtggcaacg
     4201 gagctgttgg tatcgattta atcgctacat cgggtagaga tagcggattt aatgagttat
     4261 caaatgatac taataatgat aattacattg ccaaatacaa tcagaaggtt ataaatcaga
     4321 tggtacgtgc gtatcaatat ctatcacaag ctggtcttga taatccttgg aaccctcatt
     4381 tgacatttta tagatatgta aattattctg gtttgtcatg ggacatcatt tcttctcctg
     4441 taacagagca tgatagaaaa atttatgatg aatataggaa taaattaact aatagcgagg
     4501 gcctgtataa ttattttata gatcctaata ctggttcaat aataggtttg cctcacttaa
     4561 ttgtgacgtt acaatcacag atgtttctag ccgacacaat caatgatagt gtttctgact
     4621 ttgcaggttg gttaggagat ttaatgactt gctggggtga agttaagaaa ttaggaatac
     4681 aaatggagca aggtgtattt gcattagtag gtacggctgc aggtggaaca tttagtctag
     4741 aagatttata ccaagatgtt gacgcggtaa atgtttatac cttagcaaaa agaaattctg
     4801 attcaacagc tttatcggct ttaaaaacgt actattgtga taagggtgct attgatagat
     4861 ttaatcaatt tgttaagaac agatttggtt cttcagataa aatcgcttca gtaacaaata
     4921 gtttattaga aaatgacgaa acagttgata tcttaggtgt agggataatt tcatttttca
     4981 ttgataaaat agttaataat tcacttggga cagattttac ttatcttgat tttattggag
     5041 atgaggggaa agttgcaaat ggattttctg aaaaaattat tcaatttcaa gaaaatagaa
     5101 gtaaacttaa ttaagaatcg tttactattt aaacagtgtc cttttccttt ttaaatatat
     5161 acgataaaat aagtatatga attgtaaaag gacatgatga ttatggaaga aatagaatgg
     5221 aataatctga ctcaaaataa taaaaataat tctggatgtg gaattatctt tatgtttgtt
     5281 atagttttgg gaatagtagt acttttaata actgtcaaga tagtagatta ctttgcgaat
     5341 actcctgaag aacaaataca gagaaaactt cctgagtttg aaagagtacc accttatgat
     5401 ccaagagata attcatttca agtaaaaaaa ttaaaagagt ctccaaatga aatgtggttt
     5461 gaagttacag ttgaatcaag cgacttcaaa gaaaaaaaga aagaatacaa ttatattgta
     5521 aaggcaaaag tatcaaaaag
//
LOCUS       DAHHZJ010000164         5413 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.166, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000164 DAHHZJ010000000
VERSION     DAHHZJ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.166"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(153..551)
                     /locus_tag="NBL02_002443"
     CDS             complement(153..551)
                     /locus_tag="NBL02_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCD2573109.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(548..1321)
                     /locus_tag="NBL02_002444"
     CDS             complement(548..1321)
                     /locus_tag="NBL02_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HCD2573110.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1318..2001)
                     /locus_tag="NBL02_002445"
     CDS             complement(1318..2001)
                     /locus_tag="NBL02_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HCD2573111.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2168..3055)
                     /locus_tag="NBL02_002446"
     CDS             complement(2168..3055)
                     /locus_tag="NBL02_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCD2573112.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3052..3921)
                     /locus_tag="NBL02_002447"
     CDS             complement(3052..3921)
                     /locus_tag="NBL02_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCD2573113.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4058..5344)
                     /locus_tag="NBL02_002448"
     CDS             complement(4058..5344)
                     /locus_tag="NBL02_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCD2573114.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
BASE COUNT         1666 a         1209 c          902 g         1636 t
ORIGIN      
        1 agcatatttt gaaaagaaag tctgatgctt agagtagaat ttcataaaaa tttactattt
       61 cataagtatt cacgagaaaa atacaaacaa aaaagcggaa ataacgctca tccgccttct
      121 tgtttgtgtt tatttcctct tcaaacgctt gtctatgatt cagacttttc tctatcgtta
      181 ttttcatcta aaagttcttg cattccgata atttctcgat attgatgaat cttagtgata
      241 ccaatgactt tgtctacatc aatactaaaa caaataccat tacctggaat atcgatgttg
      301 atgccttgac caatcgcatc cattacttta ttcgtcaaac tatcgggtac taatgtcaac
      361 acaatatctt tttctggcga aatgtccaaa cctaaaaatt tccctgaatc atgtacacca
      421 gaacctcttc catgtaagat cgttccgcct gctgcacctg cttcttttga aaagtcgata
      481 acatcttcgc ccattcctcc gtcaacgatc gtcacaatca tgtgaagatt caatgctaca
      541 agatctttca tttgctttcc cccaattttt gcaatctttc tttttcgatt ctgtcttttt
      601 catcttttcg tttataaata tatcctagta ctaaagtgga taatattggt gtcaatgcaa
      661 tcattgcaac cacaccaaac ccgtcaatga tcggatctct tccttcaatt tgtgcagcga
      721 ttgccacgct cattgataaa ataaatgtcg aacacatcgg gcccgttgct actccaccgt
      781 tatcaaaagc cattgctaaa aagatcttgt ccacttttcg tcctaaaata aatgctagcc
      841 cgtatcctgg gatcaagaaa taataaagag agaatccgac gagcaaacgc cacatagaca
      901 atcctactgc tgcgccgatc ccaattgaaa ttaccatcag catgattttc gatcggattc
      961 tcccctcgct caattcttca acctgtttga tcattacatg aatcgccggc tcagcaaacc
     1021 ctactaagaa ccccattaaa aagccaaggg gaaccagtat ataattgtaa ggcaatcccg
     1081 caatagcact gcctaaatgc tgtcctaccg gtacataagc catattgact ccgtgcagga
     1141 aaaggatcaa cccaatagtc gttatcaaga aacctttcat aatgtcaaag aaacgctttt
     1201 tgcgcatacg aaaactaatg atattcatta aaacaaatag gattacgata ggtgccactg
     1261 ccaaaatgac ttccatggta acgatttcga ttccatctat cacttgctgc cagaaactca
     1321 ttggaagatc acccccaaaa tcatcacggc aagaatcggc cctaacgaag caataccgac
     1381 cattccaaaa ctgtcatttt catttttctt ttgtttgatc atactagtca tccctccggc
     1441 aagggccaag ataaatggaa cagttactgg accagtagtc actccccccg aatcaaaagc
     1501 aatcggcata aattcggaac tggtgaaaaa tgaggcgatc aaaacgccta tgtagcctat
     1561 agccatcaat tgataatagg atagcttgaa tacgacccga agcagcgcaa aggctagaaa
     1621 tactccagtg ccgacgctga cgatcccaat cagcaacact ctgccaatct ctccgtcaga
     1681 tatatcatat acttgttgtc ccaaaacttg cacggaaggt tcagcaatcg tgatcacgat
     1741 cccgatagca aatcccaaac tgaccaaaac ggtcaaactt ttctttttga tcatatattt
     1801 ccctactaga tctccgactt ccatcataga ataatctgct ccaaacaaaa agagcgtcat
     1861 tccgatcatc atgattgccg caccaaccag aaatgaagcg aggtcttctc cttctaacgg
     1921 tgcaaaaaca aacgttaaaa tgacaatcag gacagtcatc ggcaatatag cagcaatcac
     1981 ttctttaaaa ttttttagca tgtatcctcc tcctttactc tttaagttta ccttaaaatg
     2041 aaagagtttt cgagcacaaa cttaaaacgt tatcatttaa tttttctcaa cataaaataa
     2101 cacacgtccc aaaagtatct ttttacttgg aaaaatgact ttgaaggaac gcgcttattg
     2161 tgttttttta ttttacagag ccgatcattc cttgaacgaa ttgcttttgc aaaacaaaaa
     2221 atacgatggc agttggtaaa gtagcgatga ctaatccagc catgatcatt ccatagtctg
     2281 gtgtatagga agcacccata gcagataaaa caagcggaac cgtccgttta tcaggagatt
     2341 gcaaagctac taagggccat aaataattgt tccagctact catgaatgtg atgattgcag
     2401 ccgcggcata ggtattttta gccgtcggca tatacactct aaaaaaaata cttagttctt
     2461 ttagaccgtc taatcgagcg gcttctacta gatcttttgg aaaagattta acattttgtc
     2521 gaaagaagaa gatcaaaaaa gcagtgctaa cagatggcag gatgacaacc aagaaactgt
     2581 tgattcccaa aaccgttccg ttcaattgtg aaaacattcg atataaaggg atcatcaatg
     2641 ccgcaaaagg aaccatcatc gatagcagta gaatattgaa aattaggtct tttcgtttac
     2701 tttgataaat ctcaaatcca tatcctgcca ttgacgaaat cagcaatgca aacacagtgg
     2761 tcaccaaagc gatcatcgct gaattccaaa gggaacgagt aaaattcagc tcattggaaa
     2821 acaaattttg aaaattgatg atcaagttac tgccaatctt tatttttccc gatgtgatat
     2881 ccacgggtgt attcgtcatt cccaaaacca tccagacaaa agggaaaatg gaaataaatg
     2941 ataggatgct taatgctgta tactttaata ctgacccaat tgtcgaacga agttctaatg
     3001 cttgtttttt gacgttgggg cgttcgattg tttctttttc taccatattt ttcatcgact
     3061 ttctcctcct actttcgttt ggattatcga caatatcaca ataaaggaaa caataacaaa
     3121 ggaaactgct gccgcatatc caaaattagg cgtgaattta taactcaaat tatagatgta
     3181 ttgagatagg gtggtcgttg catttgctgg tcccccgcca gtaatatttt gtacttcatc
     3241 aaaaagctgc aatgtcccga tcgtcgaagt aatggaagta aacaaaatga ttggttttaa
     3301 atttgggatc gtcactttga taaactgctg ccatttagaa gcaccgtcaa tagatgctgc
     3361 ttcataaatc tccgaatcaa tattttgcat gcctgaaagg aaaaaaatca tattgtatcc
     3421 agtccaacgc caagtgatcg aaagaatgat cagaactctc gcccaaattg gatgagtcaa
     3481 ccagccaatc ggtgtgtcaa tcataccaat gcccaacaac aagttgttta caaggccaga
     3541 ttgagaaaat agacttttca tgatcagaga ataactgacc atcgaagtga tacatggaag
     3601 aaagatagcg gttcgaaaca accctttata tttcaacttt ttatcattta aaaggttgga
     3661 aataaaaagt gctagaaaca acatgatcgg tacttgtaca agtaaataca ggaatgtatt
     3721 aaaaaaagct tttttaaaag ttgcatctgt caacatccgc tgataattac ctaacccgtt
     3781 aaatgtcata ttgacagggg gaccactttg aaaagacgtg atcaaagcca aaaccatcgg
     3841 aataaaaacc atgattgata tcaaaatgac tggcaagatt aaaaaagcat gcccgttttt
     3901 gaataaatct ttctgcttca ttcttttccc tccaattcta tcattgatta tctacatgaa
     3961 aagacaagaa agacaatatc tgtaaggcag acgtgatctt ttttgtcttt tcgtttgtct
     4021 gatgaaaaag cgaagaaatt cactttttta ccaccgatca attagccaat tgtgcttcta
     4081 cttgtttttg tgtatctgct agtaccttgt ctgtctcttc tccattgatg attcgatgaa
     4141 gagcttcagc tacgactgct tcaattgcat aggtctcatg tccgtaattc acttctggta
     4201 tttcagatgt ccatgttgaa aaatcgtcaa agatcttttg accactgtaa aattcagaag
     4261 cttcttgata agcttcttgt tcattcgcgc taagcatcgt agatactagt ccgatttctt
     4321 tcgcaagagt gcccatcagt tcttcattcg aagcaaacgt tgcttctagg aaatctttcg
     4381 ctgaatcttc tccagctact cctttgatca cataccagcc agcgccgcca aggttagaag
     4441 cttgcgcttt ttgcagattt tctggaagtg caggtattgg tgcaatcttc cattttccag
     4501 attggttttc tgctccttgg atcgttgaag aataccaagc gccagttggg ctagaagcta
     4561 ctgctcctga ttgtatcgca gttactccag cattccagtc agaggtttgt tcgactagat
     4621 cttctttcaa aagagtggca aacaattcaa gaccatactt taaagatgca ttgtcttgga
     4681 tcgtgactgt ctccccatct tcagatgtat accattctcc tgcctgttgc ataatcatcc
     4741 ttactcgacc aaggtcagaa ggattgactt cagcgatttt ctttcccgtc ttttctttga
     4801 catctcgcgc tacttgaata tactcgtccc acttcagatt ctccatatcc tcttctgtat
     4861 aacctgcttc tgccatcaga tccgtacgat agaagtttgc tgttacgccc gaatcgaaag
     4921 gaacaccgta tattttatct cctactttgt ttaccgcaaa tttatacggt gcaaaatgat
     4981 cttcctttac aatagcagac aaattagcga atgcatctgg ataagaagtc aaatagcctt
     5041 gtatcctata atcttcaatc aagacgatat tgggcaatcc ttctgtattc ccgctcgcta
     5101 aagcggtgtt cagtttctga acgatatcat cctgagacat cgtaacaact tctatctctg
     5161 tttcttcgtt ctcataaact ttttttgctt cattcactgc tttgatattg aatgtctcat
     5221 cccacgccca gacagtgact ttttcttctt tgtctgctgt actactagca cttccgcatc
     5281 ctgttaagaa acctgatgct gccgctaaac aaacgactcc agcaattaac tttctgatgt
     5341 tcatttttat ccgctccttt tccatccatt agttgtttac gtttgctaca ttcatcatac
     5401 caaaatttga aaa
//
LOCUS       DAHHZJ010000165         5308 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.167, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000165 DAHHZJ010000000
VERSION     DAHHZJ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.167"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            172..516
                     /locus_tag="NBL02_002449"
     CDS             172..516
                     /locus_tag="NBL02_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCD2573115.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            517..936
                     /locus_tag="NBL02_002450"
                     /pseudo
     CDS             517..936
                     /locus_tag="NBL02_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1028..1378)
                     /locus_tag="NBL02_002451"
     CDS             complement(1028..1378)
                     /locus_tag="NBL02_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573116.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1344..2675)
                     /locus_tag="NBL02_002452"
     CDS             complement(1344..2675)
                     /locus_tag="NBL02_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HCD2573117.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2931..3497
                     /locus_tag="NBL02_002453"
     CDS             2931..3497
                     /locus_tag="NBL02_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCD2573118.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3642..3729
                     /locus_tag="NBL02_002454"
     tRNA            3642..3729
                     /locus_tag="NBL02_002454"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3674..3676,aa:Ser,seq:cga)
     gene            4063..5037
                     /gene="bsh"
                     /locus_tag="NBL02_002455"
     CDS             4063..5037
                     /gene="bsh"
                     /locus_tag="NBL02_002455"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HCD2573119.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT         1911 a          877 c         1014 g         1506 t
ORIGIN      
        1 ataaaaaaag cgttagcaac tatgcttaaa aagcatatcg actgaataaa ctaagcattg
       61 gacaggctga aagactacat ttaaaatgaa atgcgaagaa acaatagaaa ataaagttgc
      121 cttagaacca tagttgaatc acctaacagt cgtgaaggga gaataagaga aatggcagaa
      181 aaacaaaccg caggacgaga tcaattagga gattttgcac caaaatttgc agaattgaat
      241 gatgatgttt tatttggaca agtatggtcc agagaagaat tatctgctca tcagcgtagt
      301 atcatcacca tatctgcatt aatttcagga ggaaattttg aacaattgcc agctcatctg
      361 aagattggca agcaaaatgg cattactagt gaagaaatag cagaaatcat tacccattta
      421 gctttttatg taggatggcc aaaagcttgg tcagctttta gtttagcaaa agaaatctat
      481 ttagaagaaa atagcgaaga taagaaagag gcgtaaatga cgacaaaaca cgaagaaata
      541 cacaatggcg tgatttttcc agcaggtgaa aaaaacgaag cttacgctca agtatttacc
      601 gggtaaagct atctgaatat gttagtgaat gatcccgaag tttctgttgg tgtaggtaat
      661 gtaacttttg aaccaggttg tcgcaatcac tggcatgtcc ataaagacgg ctatcaaatc
      721 ttacttgtga ccggaggaga aggctggtat caggaagaag gaaagccagc aagacgatta
      781 atggctggag atgtcgtcgt aacgcacaaa gggattaaac attggcatgg tgcgacaaaa
      841 gacagttggt ttgaacattt ggcgatcact gctggtaatt cagagttttt agaacctgta
      901 acagatgaag tgtatgaagg attggaaaat caataatgtc aagcttgaac agctcctgaa
      961 agtagatttt aatcgaactt tcaggagctg cttttttgat tcagtctttt ataaatagaa
     1021 aatagggtta ataataggaa tgagaagcac gtcgaaagat cttaatattg ataatcggct
     1081 gggcatacat gatttccacg tcatcaatca aactacctaa cagatgataa tcttcccgat
     1141 catgtcgggg attacctaat agttcttgat aatagatttc catactaggg tcccgaggat
     1201 tgttcagcaa atcagcaagt acatctaaat cttctggctg agtttctgcc gtaccctgta
     1261 tctctatgaa aagcccttcc tcagcaaatt ctactccaat acgtatttca gttatttcat
     1321 gggaatataa ataagtaaga agctcatcga tgattctttt tgataattct ttttccaagt
     1381 gtcttttttt cctttctgac agcggtaaag ttatctagta tcggtacaag gatacctgca
     1441 acgaaaccag ttgaaaaacc actgttatat agatttagtc ctccgtgcaa agtgctgacg
     1501 ttatggacaa gagcaatatg tagtacacca gctattacac cgtaaaaact gccgtaaaaa
     1561 ccgcttatgg gcgcgagacc tgtcccaaac acagctgcaa caagcatgcc agtactggtg
     1621 atctcatgaa aagttccaaa caaagaagca ataaatattc cgactagtat cggaaaactg
     1681 ttttttaaat gacaaccaaa agcactgaaa cccaccgcag ataaaatagc accgatgaca
     1741 ggtccattta actgaccgcc aaccagcaaa acataagaag acagcatcag accaactaag
     1801 cctatgttga tcaaagtagc accaatacca gaattagcaa tgaaatcagt tgtcaatttt
     1861 ccagatgaat cgaaaatttc tcgaatcccc aaaagccgaa aggaattata atagaaacca
     1921 attccgactg tcagcgaaaa aaagccaaaa ataatccaaa tgagaggaaa gtgatattca
     1981 ttgaaaacca atgtattttc gacgattgaa tagctgaata acctcaaaac agcagtaaaa
     2041 gtcatcgcaa tcaacccaga ggtgaatcca atattataaa gtgtgaatcc acgatggaaa
     2101 actaaagttt gagcagctaa gggcggaaga agtagaccaa ttgctatgcc agctaaattc
     2161 cccaataaaa tacctatcag taaaggaaaa cgcataccaa aagtgatata actaacgacc
     2221 ggtgataatg cgctaccaaa cagtccgatc atgatgtact gagaaaaagg tttattgaca
     2281 aatacagcgt atagatacac acctaaaatg attgagatag aattataaag gtttttgcca
     2341 aaaaaagaga aaccggtaac tgttagaatc gaagcaaata agggaccgtt taaaatcacc
     2401 ttgtgccgat aagctaaaag cacagaaagt aaggtcaaaa tccctacatt tatgaatgcg
     2461 cttccgaaac cgcctaaagc aaaataatct gtcaaaagat tgcttggcga ggtaagaata
     2521 gttagcaatc cactccaatt atcagtagga gtattagaaa gcaagccaat aaaaagaaaa
     2581 aaaacacctg taaaaccaag ataaaaatac tttcttttct gtgaaacgat tcgtttttga
     2641 ggatgaatat aaataacgtc agtttcgttt tccattacac accttctttt tcattttctt
     2701 taactataac ttgaaatgaa agatttggac aactatcatt taggtcaaaa ctaaaaatgt
     2761 aagggaaccc attgaattta accaaatttt tttaattttg caaaaaaaag ttaaagaaga
     2821 ctgaaaaaaa atttaaaata taacgttttg agtaaaatgt ttgcaaaaaa tgctaaaaat
     2881 agggtacaat aaaacaacat atactgcagt aaagaagtgg gggagtttta atgagaagaa
     2941 aagtctatac caaggatcaa attctaaaag ctgcttatga tgttgttgca aaggaagggt
     3001 ttaaaggatt tactgcgaga aatattgcta aaaagatggg gatttcgaca cagccgattt
     3061 atctggaatt caaaaacatg gaagatttaa aaaatacgtt gctggattct atctttgaac
     3121 aactaaaaga agatgttttc cctattgaac gtactggtga taaaatcatt gatctcggtt
     3181 tgaactatat ctactttgca aaaaagcatc atagtcttta catggcattg tacattgatg
     3241 aacatgggga cggaaagaaa atgcatgatt tttcatacaa ttatttccgt gaaatagtta
     3301 aagaagatga aagatacaat caattatcgg atgaatatgt cgatgctctg catactggta
     3361 catgggtagc agtgactggc ttagcgtcat tgatgtcatc aaacatcatg catccaacag
     3421 aagaacaaat cattgcactg atcaaacgga caattgactc gattttagca ttagataatc
     3481 ctgaaataat cagatagtgt tctatcagta tgaatttgat cggtaagtcc gttggctgca
     3541 aactgtcaat ggactttttt gctaaaaaaa gcgatttctg ttatttatct attagaaata
     3601 ggcttgacat ttcgtgaaaa aagtttaaaa atagtactta cggaaggttg gcagagtggt
     3661 aatgcaccgg actcgaaatc cggcgaaccg gctaataccg gcgcgcaggt tcaaatcctg
     3721 taccttcctt atatcaacga ttagaggttg tgaagaagct gtcctgcatc aagtaataag
     3781 aacggccaaa caaaaatagc ttttcctatt ttttcgtttg attgggctta ttaccgcaga
     3841 tgcaagcttt tgaacaccgt ttgttcggaa taaggggctg tgacaagact tttgtcacag
     3901 ccccttattg ttgtactttt aacctacaat attttttgat tccccctgag tactattttt
     3961 tataaatgct agtaaatcaa tcttctttgg cctttttttg ataaaaaaaa tactagatgt
     4021 aagataaaag tgtaaaaacg atttgtttta ggacgggaaa cgatgtgtac cgcaatcact
     4081 tatgcaacga aagaccatta ttttggaagg aattttgatt atgagatgtc ttataatgaa
     4141 gtggtcacta ttactccaag aaattatcgt ttcgatttta gaaaggtaaa aaatttagat
     4201 aaacattatg caatgatagg aatagcagct ggggtatcca actatccact ctattatgag
     4261 gctacaaatg aaaaagggtt gagtatggca ggactgaatt ttcctggcaa tgctgattat
     4321 aaagaattac aagaaggaaa agataacgtt gcaccatttg agtttattcc atggatacta
     4381 gggcaatgct caaccataga cgaagctaaa gaattattgg ctactatcaa tctagtcaat
     4441 atcgatttta gtgaaaaact acccttgtca cctttacact ggttattagc tgataaagaa
     4501 aaatcgatag taattgaaag tatgaaagat ggacttcatt tatatgataa tcctgtcggt
     4561 gtgttgacaa ataatccgcc atttgattat caattattta atctgaacaa ttatcgctcg
     4621 ctatcaaatg gaacaccaga aaatcatttt tcaaaccaga tcagcttgaa tgtttatagt
     4681 cgtgggatgg gaggattggg cttacctgga gatctctctt ctgtttctcg atttgtaaaa
     4741 gccacattta ctaaaatgaa tgctgcatca ggtgattcag aatctgaaag tatcagtcaa
     4801 ttttttcata tactaggctc agtcgagcaa caaaaaggag tatgcgatac tggagaaaga
     4861 aaatacgaat atacgattta ttcttcttgc tgtaacgttg ataaagggat ttattattat
     4921 cgtacgtatg aagacagtca aattactgct gtaaatatga ataaagaaga cttggatcgt
     4981 catgaattgg tcagttatcc agtcataaaa gagcaacaga taaactatgt caattaatta
     5041 atgaatagat ccgtttggta tcatattttc tgatacagtc ataattagac acgatcttta
     5101 ggtcgtgtct ttttttgctg gtttttttgg aaatagcatc cactaatatt tttttaacac
     5161 gaagagaata aggtagcctc aaaaaagaaa acatggtaaa ttagatattg ggactttatt
     5221 ttctgtaaaa acagaaactt tcatcttttg ttttcacgtg acgaaattgt aagagagaaa
     5281 atattcgtcc gctatttatt ctatgata
//
LOCUS       DAHHZJ010000166         5297 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.168, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000166 DAHHZJ010000000
VERSION     DAHHZJ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.168"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            12..84
                     /locus_tag="NBL02_002456"
     tRNA            12..84
                     /locus_tag="NBL02_002456"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="NBL02_002457"
     tRNA            88..160
                     /locus_tag="NBL02_002457"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="NBL02_002458"
     tRNA            168..249
                     /locus_tag="NBL02_002458"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="NBL02_002459"
     tRNA            267..339
                     /locus_tag="NBL02_002459"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="NBL02_002460"
     tRNA            351..422
                     /locus_tag="NBL02_002460"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="NBL02_002461"
     tRNA            439..524
                     /locus_tag="NBL02_002461"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="NBL02_002462"
     tRNA            535..608
                     /locus_tag="NBL02_002462"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="NBL02_002463"
     tRNA            618..691
                     /locus_tag="NBL02_002463"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="NBL02_002464"
     tRNA            711..783
                     /locus_tag="NBL02_002464"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="NBL02_002465"
     tRNA            811..884
                     /locus_tag="NBL02_002465"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="NBL02_002466"
     tRNA            898..971
                     /locus_tag="NBL02_002466"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="NBL02_002467"
     tRNA            995..1084
                     /locus_tag="NBL02_002467"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="NBL02_002468"
     tRNA            1097..1170
                     /locus_tag="NBL02_002468"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="NBL02_002469"
     tRNA            1189..1264
                     /locus_tag="NBL02_002469"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="NBL02_002470"
     tRNA            1266..1336
                     /locus_tag="NBL02_002470"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="NBL02_002471"
     tRNA            1350..1423
                     /locus_tag="NBL02_002471"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="NBL02_002472"
     tRNA            1448..1535
                     /locus_tag="NBL02_002472"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="NBL02_002473"
     tRNA            1557..1628
                     /locus_tag="NBL02_002473"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="NBL02_002474"
     tRNA            1651..1740
                     /locus_tag="NBL02_002474"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="NBL02_002475"
     tRNA            1753..1826
                     /locus_tag="NBL02_002475"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="NBL02_002476"
     tRNA            1845..1917
                     /locus_tag="NBL02_002476"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="NBL02_002477"
     tRNA            1921..1993
                     /locus_tag="NBL02_002477"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="NBL02_002478"
     tRNA            1998..2068
                     /locus_tag="NBL02_002478"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="NBL02_002479"
     tRNA            2081..2154
                     /locus_tag="NBL02_002479"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="NBL02_002480"
     tRNA            2166..2237
                     /locus_tag="NBL02_002480"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /locus_tag="NBL02_002481"
     CDS             2503..3696
                     /locus_tag="NBL02_002481"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCD2573120.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..>5297
                     /locus_tag="NBL02_002482"
     CDS             3826..>5297
                     /locus_tag="NBL02_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD2573121.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
BASE COUNT         1377 a         1020 c         1324 g         1576 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagta
//
LOCUS       DAHHZJ010000167         5294 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.169, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000167 DAHHZJ010000000
VERSION     DAHHZJ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.169"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            81..1559
                     /locus_tag="NBL02_002483"
     CDS             81..1559
                     /locus_tag="NBL02_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD2573122.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            1836..3896
                     /locus_tag="NBL02_002484"
     CDS             1836..3896
                     /locus_tag="NBL02_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HCD2573123.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            3979..4548
                     /locus_tag="NBL02_002485"
     CDS             3979..4548
                     /locus_tag="NBL02_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD2573124.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(4723..>5294)
                     /locus_tag="NBL02_002486"
     CDS             complement(4723..>5294)
                     /locus_tag="NBL02_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573125.1"
                     /translation="SFIFSSTVLGELSDFYGYFSMWDTFLHAFSGFLAGGVGFSLVYL
                     LNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSADKLLQFDMQKDTIIQQINSVE
                     LSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGYLDVGIMDTMKDLFVNFIGAVV
                     FSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
BASE COUNT         1905 a          882 c          964 g         1543 t
ORIGIN      
        1 taaaatcagt ataggtgaaa agatgaactt tcatctgcat atcaagtaat tggtatgagg
       61 cattttttga ggtgtaggat atgaagttgg attaccttat agaagaggaa gatagagaaa
      121 aactgctgct agctagatat ttagaatttg agaaaaagaa ttatgtagtg ctgcctaagt
      181 tatatgaaac attaggtttg tctaaattta aaacaagaaa ttatatcata gaattaaata
      241 aagaatttac tgaattcaaa acaaagccag agatccaaat ttcagaagaa gcagagatcc
      301 atgtcgaacg tctgaatttg acggatatca aaaatttaag aagaatctac tttcaacgat
      361 caaaaattgc tcgactgtta acggagatcg tggattcaaa agtaacgatc gaaaaatttg
      421 cagagaatca ttttttaagc atatcaagag cctatgtaaa aagaagagaa ttagtctctt
      481 ttcttgaaca gcatcaaata aagttaaaga aaaacatatt agttggggaa gaaataaaca
      541 tacggaacgt attatttggg gtatatttcg gggtctttaa tggtttttct atgccattta
      601 gagaaagcgt catgaatgaa gcaaagagta tcgtgcaata ttttgaatat ctgttcaatt
      661 taaaactgtc tgcaacaaaa cgaatcaaat tcaatttgct aaccactatt agtttgttga
      721 gggcctatgg gaatcaacca atcggaaaac gtttcttttc agatgaatac tttaagagct
      781 cagaaggaat gaagatcatc aaggatttat cacatatttc ttttcttcat atccctaaag
      841 agcagatcat tgatgagatt tcttatatca tgttgttttt gatcacggaa gaagaatttg
      901 aattagatgg cgcatttgga cttgcggaag ctcattttca tgatatccac atgattagtc
      961 aaaaaatgtc agtggaattg attgatcaat taccattttc gattgaaatc tctgaagaag
     1021 aaagactata tatacgaaaa gaacttcgta tgggtatgat gcatgtcaat cgaaaaaatt
     1081 ggatattcga ctttgagatg actagcttta cgtctaaaaa gcagctacag tttttcttcg
     1141 agacgtatcc aatattcagt acagcaatcc aaaaggtcat acaagagaat aaacgctatt
     1201 tcttgggaaa agatgaacgt ttcatgattc gcttatttta tgattatctc tttttattgg
     1261 tcggtatttg tccggtatca tattttgaag caccgattca tgtctgtatt gatttttcgc
     1321 aaggatctgc ttataccgat tacattattt ctcaagtgaa aggatttaaa aattacaata
     1381 ttgtgattga gagtcgaatc acttctcgta cgaatattta tttatctgat tgtttacttg
     1441 aaaaatttaa taaagaacag ataatttgga aaaatccacc gactccagat gactgggaat
     1501 attttggaaa taccatcatc agagaaaaaa atagacattt aactgaaaaa atcagctagt
     1561 atttatggat ggcagctttg tctcacatat ttattagctg atttatgttt ttttcaaaga
     1621 aaaggtaact agatataact agatataact agatatatta cttaacaaaa ttaaaaagta
     1681 cataagttat tattccgatt ttcttgaaac aataaaaaat caaaaaaaat actctcctaa
     1741 tagtccatct tcttttgatt ttatttatat aatgaaatca tcttatgaaa attactactt
     1801 catgtttaga caagagtagg gaggactaag aataaatgtg ttcaaaaaga cgttcaaaac
     1861 aactaaataa acacgcttgt cgtttagata aggaacggtc gtgttctgaa aaacactacg
     1921 ttaagtttat gatgcgcacg atgctgatca gtttatctgt aggtgtctat atgcatgtac
     1981 ccgttcaggc aacgacacaa accatcaagg tcgtacgtac ctctgtttct gagaccactg
     2041 attcccctat cactattact catactttca ctgatgcaaa tggaaaacag ctacttcctg
     2101 cattgacttc ctataataaa gtcagttatc ctccaacgat aaaccactac tggttaattc
     2161 ctaacaaaac attatggaca tcggaaaccg gaagcagtgt gccgctttct atcttttccg
     2221 atatgactga tcctacaaaa ggtgatcagt taaaacagat cactgatatt ttgaatcatg
     2281 ctttgacaga tataaaggac ggtggaagcg cacaagtttc ttatgtatat gaaaaagatt
     2341 attcacagtt tgaaagaaag aaaatcgatg ttctggcagg gacaaaagtt tcgatatctg
     2401 atctggtaga aaggctgaag aacgcagatg gaacacaagg agatatgtcc aaagtaattt
     2461 caaacaatgg gaatatcgtg gatacgacta atacaggaac atctactatt tccttgtcct
     2521 attttgatcc aattgcttta aaaacaatga ctgcatctgc tgtgatccat gttcgtgctg
     2581 atcaaacagc tatcaaagga aaagatcttc acttaagaac aggagagagt tacgccccta
     2641 aagatcttgt tgaatatgta acaaaaagcg atggaacttc aggaaataaa gaagaaatca
     2701 aaatgaacgg gacagtagat actacaactc cgggagaata tcccgttgta ttaacttata
     2761 tagactcgaa aacatcaaat gtggcagaaa caacagctgt agtttatgta gaagcatctg
     2821 ctgcttcatc gactgtcatc actcatttta tagatagtca aacaaataaa gaacttcaag
     2881 cagaaacaca tatgagtagt gtaaatcgta ttcctgattt gcgcctacct gctgaatttt
     2941 ctgatcgagt gccaaatata gatttatcga cttatgaaca aaatgggaaa gtcacttcta
     3001 tttctgattg gatgcaagca aacggattag acagaaaaag tcactggtcg gagatccttg
     3061 aagtacttca ctcagaactg atgaataata ggaagcaatc aggaggggaa attaccttta
     3121 cttatgttta tataccagac aatcgttcat tagaaggtca tgatcttatt gttcatccaa
     3181 acgaaaaaat aaacaaagaa gagttgatcc aatcagcaac aaattcctta ggtaaacccg
     3241 taccgttaaa ggatgtatcg gttacaatca atgaagtacc attggaggat ggttatttga
     3301 ccaaacaaac aggtgatttt acgattatgt atagttatca tgacccgtat tcactccaag
     3361 aactccatgc tcagtcacta ttacatgttt taccattaaa caaacagata gatcaaaaag
     3421 aacaaaaaag acaagaagaa agcttgtcag aaaaaaaaca acaaatggcc catgcagttt
     3481 ctccaacagg gaaagtaaaa caaacaagaa gcctagagtt agcaagaata tctagaaaaa
     3541 tctcagaatc taataaaacg ataaggcaag aagctcattt ggccccacaa gtttctggat
     3601 tggaaaatga aattttagag cctaaaataa aacaacaatc agaaaaagaa tttgttgcta
     3661 aacagtcgga agatcagaag gaatcagatg accagaagga aacgaagaaa aaacgcttgt
     3721 caactattga ttccaccgat gagcatctgc agccgcaagt accacttcct agtccgggaa
     3781 cacccgcacc tggtggaggt attccttctt ctgctttagg agatttcatg cgaggtatgg
     3841 caggaacatg ggtatacgct aatcgaaatg aatttgacgc agatgactgg ttctgattta
     3901 tatctctatc tgttatagtc aaataagtag ctgagtcata tgttatttgg cataatggag
     3961 acgaggaggg atagaaaaat gttagataaa acgttacctt acgcagaaat ctggatgaca
     4021 agaccacttc aacaaaatgt tccaatcacg cctttagcgg aaggttttga atttcgattt
     4081 tatcaaaaag aagatgaatc tgcttgggcg gagattgaga cttccgttgg ggaatttcaa
     4141 acgacagatg aagcacttga gtattttcaa caaaactttg ctccttatcc tgaagaactt
     4201 gaaagaagga tgttttttgt agagacaaaa aaaggagaaa aagtagcaac ctgtaccgct
     4261 tggcgaaaaa aatcaagaga gcaagtgtat ccggtttttc attggttagc agtgaaacca
     4321 gattaccaag gaaaagggct cgcaaaagca ttgactgcca gagtcttgca agaatttcct
     4381 accctccata cggacggacc aatctatctt cacacacaga catggagcca tcaggcgatt
     4441 gctctttata aaaaaatggg ttttactttt attgctgaaa atctagacgg cacacctaat
     4501 ccagattatg aaaaagttat gcaggtttta agtaaattag ataaataacg aaatggtgaa
     4561 ataagtcctc agtattggga taaaaaacga aatagtcatt cctcatatct tacttttttg
     4621 agttaatact atcggataac taatggcatg ccatacattc aaaaaagaag gggcagtgac
     4681 aaaagtattg tcactgcccc ttctcttgtt ttttagcatg ttttatcgtt tgattttttt
     4741 aggaataaaa ttcttgatga acgtaaattt ttggtaatcg tttcgactat agaggtaacc
     4801 aaatacactg aaaacaactg ctccaataaa attaacaaat aaatccttca tagtatccat
     4861 gattcccacg tctaagtaac cattttggat gattgtttta tctgttttac catctgcatt
     4921 ttgactttca atgattgtcc gagtgatatc tggaacatga tagacagtgt ttccgtcttt
     4981 acttaactcc acactattga tttgctggat gatcgtatct ttttgcatat cgaattgcag
     5041 taatttatct gctgaaaatt ccacaaactc ccacataaca ccaactgtca tcgaaaaaca
     5101 gaaggaaaca actgctagta ataaaggagt caagttcatg gtttttgtat ttttattgag
     5161 caaataaaca agggaaaagc cgacaccccc agctaaaaat ccgctgaatg catgtagaaa
     5221 agtatcccac attgaaaaat agccataaaa atcacttaac tctcctaaaa ctgttgaact
     5281 aaaaataaaa gata
//
LOCUS       DAHHZJ010000168         5272 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.170, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000168 DAHHZJ010000000
VERSION     DAHHZJ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.170"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..1497
                     /locus_tag="NBL02_002487"
     CDS             <1..1497
                     /locus_tag="NBL02_002487"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HCD2573126.1"
                     /translation="FYKYLSDRLLEQVVLLADESLEEYDTVSKQTMLYRELLSDEESK
                     EDLIATIVDILGYAIAPEYLFNVLADQAKQATFQLNDLNKAFVQLASTYNQFNGLFDD
                     VDLQSKKLGTDEQQRNVTITEVIKKLNDVDVLEHDGDVIGDAYEFLISQFASEAGKKA
                     GEFYTPHMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQ
                     ELNTTTYNLAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWS
                     ADTTFLDDSRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGV
                     IRQKLLEDGSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQ
                     NKLSEENIQKILETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVDTFEEEEPIDMV
                     HVGNDIKKIRQEQQVLEKKLLEAISSLQTTPENEAWLQGALEVFKHEQ"
     gene            1487..2704
                     /locus_tag="NBL02_002488"
     CDS             1487..2704
                     /locus_tag="NBL02_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCD2573127.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            2717..>5272
                     /locus_tag="NBL02_002489"
     CDS             2717..>5272
                     /locus_tag="NBL02_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HCD2573128.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQ"
BASE COUNT         1842 a          908 c         1092 g         1430 t
ORIGIN      
        1 ttctataaat atttatccga tcgattgttg gaacaagtgg ttcttttagc agatgaatcc
       61 ttagaggaat atgacacagt aagcaaacaa acaatgttgt atcgtgaatt actttcagat
      121 gaagaatcaa aagaagatct gattgccaca atcgttgata ttttgggata tgcaattgca
      181 ccagagtatc ttttcaacgt cttagcggat caagcaaaac aagcaacgtt ccaattgaac
      241 gatctaaaca aagcctttgt gcaattggca tctacctaca atcaatttaa tgggttattt
      301 gatgatgtgg atctccaatc aaaaaaacta ggaacagatg agcaacaacg aaatgtgacg
      361 attaccgaag tcatcaagaa attgaatgat gtagatgttt tagaacacga tggggacgtg
      421 atcggggatg cgtatgagtt tctgatcagt caatttgctt cagaagcagg gaagaaagcc
      481 ggagaatttt acacacctca tatggtttct gatatgatgg cgcaaatcgt aacgctcgat
      541 caaaaggaac gacgattctt tagtgttttt gatccaacga tgggttcagg ttcattgatg
      601 ttaaacgtac gtaactactt aacacaccca gataacgtta aatatcatgg gcaagaacta
      661 aatacaacga catacaatct agcaaaaatg aacttgattt tacatggtgt cgacgccgaa
      721 gaaatgaatc tccgcaatgg ggatacgttg aataaggact ggccaacaga tgaaccttac
      781 acatttgatg cagtagtaat gaacccacct tattcggcta attggtcagc agatacgact
      841 ttcttagatg attctcggtt taatcgttat gggaaattag cgccaaaatc caaagcagat
      901 tttgcgtttc ttcttcatgg gttctatcat ttgaaggaaa caggtaccat ggccatcgtc
      961 ttaccacacg gagttttatt ccgtggcgct gcagaagggg tcatccgtca aaaattacta
     1021 gaagatggca gtatttatgc tgtgatcggt atgcctgcga acttgttctt tgggacatcg
     1081 attccaacga cggtgattgt gttgaagaag aatcgtcaaa ctcgagatgt attattcatt
     1141 gatgctagtc gtgaatttgt taaagggaaa aatcaaaata aactatcgga agaaaacatt
     1201 caaaaaatcc tcgaaaccta cgctgaaaga aaagatgtcg agaaatacgc tcatttggca
     1261 acctttgatg aaatcaaaga aaatgactat aacttaaata tcccacgcta tgtagatact
     1321 ttcgaagaag aagaaccaat tgatatggtt catgtcggaa atgacatcaa gaagatacga
     1381 caagaacagc aagtgctaga aaaaaaactt ttagaagcta tctcttccct gcaaacaaca
     1441 cctgaaaatg aagcgtggtt acaaggtgcg ctggaggtgt tcaaacatga gcaatgatac
     1501 acaaccagaa atccgtttcc caggatttac agatgattgg gaacagagga agttggggga
     1561 cttcgttgta gattatgttg aaaagacaag tgtacaaaat caatttccta tgctaacttc
     1621 atctcaacaa aaagggatag ttttgcaaga agactatttt gcaaatcggc aagtaactac
     1681 agaaaataat attggttact ttgtactacc tagaggatat ttcacgttcc gtagtcgaag
     1741 tgataatgat gtgtttgtgt ttaaccgtaa tgacattatt gatagaggga ttataagcta
     1801 tttttatcct gtatttactt taaaatctgc tgattccgac ttcttcttaa gacgtattaa
     1861 caatggaatc caacgacaat tatctattca agcagaggga acaggtcaac atgttctgtc
     1921 tctaaaaaag tttaaaaata tagtagcaat gtttcctagt gaggaagaac aacaaaaaat
     1981 cggcaccttc ttcaaacaac ttgacgacac gatcgctctt catcagcgta agttagattt
     2041 gctgaaggaa acgaaaaaag gattcttaca aaaaatgttc cctgaaaacg gagcaaaagt
     2101 gccagaaatc cggtttcctg gttttacgga agattgggaa gagcgtaagt tgggagaatt
     2161 aggtaaaact caatcgggaa ttggatttcc tgatgccgag caaggtggtt ctgaaggtac
     2221 tccatttttt aaagtttccg acatgaataa cattggtaat gagtatgaaa tgagaaatgc
     2281 aaatcattat gtatctaatg agcaaattga gcgtaaaaaa tggaagccaa taaaagatgt
     2341 gccagcgatt atatttgcta aagttggcgc agcaataatg cttaatagaa aacgtttggt
     2401 cacaagccct ttcttaatag ataacaatac catggcttat ttatttaata atacttggga
     2461 tatctatttt ggtaaaatat tgtttgaaac tataaatttg ccaagatact cccaagtagg
     2521 ggcattgcct tcttataaca gttctgatat tgagaatatc agtgttaagg ttcctgtaaa
     2581 ggatgaacaa caaaaaatcg gtactttctt caaacagcta gatgatacga tcgctcttca
     2641 tcaacgtaag ttagatttac taaaagaaac gaaaaaaagc ttcttacaaa agatgtttgt
     2701 ctaggaggga agaagaatga aaattagtca ccgtgaggaa gcagaagtcg aagaacagtt
     2761 gattcgtgta cttggagaag ggcataatca gtggacgtat cgtccggatc taaaatcaga
     2821 agaagacctt tgggtcaatt tacgccaaaa aattatctca aataatcaag cagaactgaa
     2881 tgattttcct ttgaccgata aagaatttga aacgatcaaa acggaattac tgctgcgaac
     2941 gaaaacccca tttgatgctg cgaaatggtt aaaaggtgaa aatgggatgg ctcggatcac
     3001 gattgaacgg gaagattcgc aattgggttc tgtctcactt atcctttatt ccaatcaaga
     3061 cattggtgga ggaatctcta cttatgaagt ggtccatcaa attgcaaaac gtggaagtaa
     3121 catagaagca cgggatcgac gttttgacgt caccttatta atcaatggat taccgattgt
     3181 ccaaatcgag ctgaaacaag taactgcaaa agatggtgtt tatcaggcat ttaaccaaat
     3241 caaaaaatat gcagaagaag gtatgtttcg gaacaatatc ttttcgacct tacagttgtt
     3301 tgtggtttcc aatgaacaaa ccactcggta ttttgcaaat gccttaccga aagacttgca
     3361 ccctaagttt ttatttagtt ggcgaacgaa agacaatgaa aaagtagaaa acttatatga
     3421 gttttgtaaa caagtgttaa atatcccgga tgctcatcgt ttgattgcgg attatacaat
     3481 cgtcagtgaa gatcaagaca ataaaaccct catggtgtta catccttatc aagtacatgc
     3541 gattcaagcc ttgtttattg ccgcaaataa acatcaatca gggtatgtgt ggcatgcgac
     3601 tggttcagga aagacgttga ctagttttgt atctactaag cttttagcac gaaaatcggg
     3661 gatcgatcga acgatcatgt tagtggaccg caaagattta gataatcaaa ccaccacaga
     3721 gtttacaaaa tttgcatcgg aatttaatac tgggatttct tccgggaacg ccaaagcgaa
     3781 tagcttgatc gttggaacgg gaagtgccaa agaattaagt gaaaccctgt tggcagatgc
     3841 caatgcaaat gttgtaatca ttacaacccg tcaaaaacta gatgcggcgt tgaaatatgc
     3901 aaagaaacaa gaagagaaaa aaggaaccaa tcgcttccaa aaattaatgg gccaacatat
     3961 cgtgtttgta gtagatgagt gtcatcgagc gttaagtgct gaaaatatgg aagaaatcaa
     4021 aaaaatgttt ccgaaatcca cttggtttgg ctttacaggt acaccgattt ttcctgagaa
     4081 tcggaaacaa gccaaagggc aattggcacg tacaacccat gatcagtatg gagaggtctt
     4141 gcatacctat accattaaga atgcgctaga agatggctct gtcttaggat ttcaagtaga
     4201 gcatgaaaac accgtggagc cgacttcact agagaataaa atctatcgga aattaaaaga
     4261 agtagaaaca tatgcagagt attcatcaga acagatcaat cgtatgattg atcaaatgga
     4321 acctgtcaaa aaagaaagtt atttggaccc agctgttttt gaagcagatg aacatatcca
     4381 aaaagtcatt cataaaatgt tccgtccaga taatgcctat acaaaatttg attttcgaaa
     4441 tggcaggcca acgaaatctg caatcttaac cacgagttcc attgacatgg ccaaaaagta
     4501 ttatcgggcc atcaaagaga tgacgaaaga gcctgattgg ttaacaaaag agtttcccaa
     4561 tcagctcatt cgtgaaggac gaacgatgga ggatcctgat tttccacgaa tcgctattac
     4621 ctattcatta gaagaaaata gccgagattc aagtgcccaa caagaagaaa tgcaaaaaat
     4681 tattgaggaa tataatcagt attatgatac ttcctggtca ttagcagaca ttgagcgtta
     4741 taacggagac atcaataatc gtttggcacg aaaaagagcg gaattcaaac aatttgggaa
     4801 acaaatcgat ttagcgattg tagtggatcg tctattaaca ggttttgacg cccctacgat
     4861 tcaaacccta tttgtggatc gtaatctaga gtatgcagga ttgatccaag ctttttcacg
     4921 tacgaatcgt acgtatcctg ataagacaaa aggattgatt gtgacgtttc gtaagccagc
     4981 gacaatggag aagaatgtcg aggatgcgac aaaactgtat tcaaaagcaa aagaagaatc
     5041 aggtttgatt tatccaagtt atcaggaatc caaaaaaaga ttcaaacaag catatggtaa
     5101 gttaaaagag gtttcatcca tggaatccat tgatgaacat acgccacttg aaatccgtat
     5161 cgagtacgtc aaagcatttc aagaattgaa tcgtgcttat gaatcgttag tgacttatga
     5221 cgattacaat gatgacatgg tgaaatctcc aactttgaac aatcaaattc aa
//
LOCUS       DAHHZJ010000169         5243 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.171, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000169 DAHHZJ010000000
VERSION     DAHHZJ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.171"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(<1..518)
                     /locus_tag="NBL02_002490"
     CDS             complement(<1..518)
                     /locus_tag="NBL02_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD2573129.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNVASIVMSTIFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVA"
     gene            729..1175
                     /gene="nrdI"
                     /locus_tag="NBL02_002491"
     CDS             729..1175
                     /gene="nrdI"
                     /locus_tag="NBL02_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCD2573130.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEYEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQRFGFPFLADYELRGTPSDVTRVYQILAEKNK"
     gene            complement(1245..3227)
                     /locus_tag="NBL02_002492"
     CDS             complement(1245..3227)
                     /locus_tag="NBL02_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCD2573131.1"
                     /translation="MAKNELTLEELAKQIYQGAGGMGNVESIVHCMTRVRMKVINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQEMVDLAGVKLGEKLPSASATTAS
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAGTWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALATGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKAVPQASA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVISSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAADK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(3241..4137)
                     /gene="murQ"
                     /locus_tag="NBL02_002493"
     CDS             complement(3241..4137)
                     /gene="murQ"
                     /locus_tag="NBL02_002493"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCD2573132.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELPSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISEHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEAAQAKLAEANGFVKGTL"
     gene            complement(4140..5201)
                     /locus_tag="NBL02_002494"
     CDS             complement(4140..5201)
                     /locus_tag="NBL02_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCD2573133.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQNRLIELGSEAKKNKLELMVDISGEALKRAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPNDIDELKEAAADFSQIEAWHNYYPRLETGLDKEWYQEK
                     NQWLKTQGFLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSEHVQEQFASFQKEQTVLLHTEPVDEVFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPVRNTAKRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
BASE COUNT         1456 a         1201 c          930 g         1656 t
ORIGIN      
        1 gctactacaa taaataaaaa taatggcact aatttacaaa atgtgacgat agcattgaca
       61 aagctagcat tttcaactcc acgattcact agccatgcat atccccataa gaagatcgta
      121 ctcatcacga tcgatgccac attttgacca ccttcgaata cggggaagaa ataacctaat
      181 gcactcatca acaatgttgc gaacgccacg tttcctaacc agcatgtgag ccagtaaccc
      241 caaccgctga taaaaccagc gaaacggcca aaaccttctt ctgcgtaact aaagatcccg
      301 ccttttaaat ctggacgttt tttattgata ttattgatcg ataaggagag catcaacatt
      361 gctgttccta cgagtaccca gctgaagata gctgggccac cagccgatcc ggagatgtcc
      421 gtcataatac caaatacgcc acctccaatc gctgaaccaa tcactaaggc agcaagtgga
      481 atcaaaccaa ttcctttttt ctgttgctct gtatccatgt aagcatcctc caaagtaaaa
      541 ccgcttttat caaaaagtat aacaaacctt ttttattttt acttaattaa taaaaacttt
      601 aatagaaata taattctacc gacttttttc tcattataca agcaaagcgc ctagtccctt
      661 tccaaattac accaccaaaa aaacttaaaa agatgctata ctaaagggaa agaattgggg
      721 gatgatccat gaacatttta tatatatcga tttcaggaaa tacgcgtgct tttgccaaac
      781 atctagcaga atatgctgaa aaaatgcatg cagaagatcc tgtcaatcct gaagttactt
      841 taaaagaaat ccatgaaaat agtgatttta cgaaagaaac agaaccattt tttactttcg
      901 ttcctactta tttagacggc gggaatgggt tagacaacgg tgacacagaa atcttgaccg
      961 aaacgatgcg cgaatatctg gaatacgaag acaatcataa actttgcctt ggtgtagtcg
     1021 gtagcggtaa taaaaacttc aacaatcaat attgtctgac agccaaacaa tacgcacaaa
     1081 gattcggctt tcctttcttg gcagattatg aattacgggg aacaccatct gatgtgacac
     1141 gcgtttatca aattttggca gaaaaaaata aataacacaa aagaagaagc tgaaaaataa
     1201 atttcggctt cttcttttta tgttctctat tgtttctatt cttattaagc tttgacatcc
     1261 atcacaggtg cttttgcttt cgctttacct gttttttcca agacaaagtt ttttacacga
     1321 tccatattgg ttaccaagac gatcatggag gtttctttgt ctgctgcttt gattgcttct
     1381 aagtcagctt ctgctacaag cgtatcaggt gtcacttgct gtccttctgt tactttcaca
     1441 tcgaatggcg taccgcctaa gtcaactgtg ttgatgccca tgtgaagcaa gatttctaag
     1501 ccatttggtg tcgtaatgcc gatagcatgt tttgttggga aaacagaact tattactcca
     1561 gtaaccgggg aatagatatg tccgttttct ggttctacaa agtaaccttc acccatcatt
     1621 ttttgtgcaa aaactgggtc acttgcttta tctaaagaaa caactgttcc gtcagcaact
     1681 gatgataaga tagtcacact ttcagctgaa gcttgaggta ctgctttttc aggtacttca
     1741 tttactgtgg ctgtttcggt cgctagttct tctttttcta aacggtcttt aggaataccg
     1801 aagaagaatg tagcaacgaa gccaccagcg taagcagcca ataaaccaag gatatagcct
     1861 aaccatttac catcagagat caatggaatc aaagcggctc ctgaaggtcc aatagcgatt
     1921 gctccgacat ttccgatcat tccgatcact gccccaccga tacctccacc gatacaagca
     1981 gtaataaatg gacgtcctag aggtaatgtt actccgtaaa tcaatggttc gccgattcct
     2041 aagattccga ctggtaaagc ccctttgatc atttctgtta attctttgtc ttttttgcag
     2101 cggatccata atgccaatgc tgcacccact tgtcctgctc cggccatcgc aaggattgga
     2161 agcaataagg tacgaccagt ttcatcaatc atttgtacat ggattggcgt caagatctga
     2221 tgtagtccga acatgaccat tggcaagaat aatgtaccta gaacgaagcc ggcaaatgct
     2281 cctccaacac caagtaccca gttgatccca ccgaccatgc cgtcagaaat gaaccctgct
     2341 aaaggcatga tgaagaaaat ttcgatcaga ccgatggcaa tcagggaaag agttggcgta
     2401 acgatgatat ctaccgcatc aggtactact ttatgcagtt ttttctcaac gattgacaat
     2461 aaccaaactg cgaagataac acctaagatc ccgccttgtc cagttgcaag agcttcaccg
     2521 gtaaacaagt ttttcagtgg tgcttctgga ttcatccctg tcagcatcac aacagccccg
     2581 ataactccac caagcgttgg cgttgcacca aaaacttgtg cagcattgat acctgtatag
     2641 ataactagat agctaaacat cccgtttttc aagatgttca tcacatcgat atattgttgc
     2701 catgttcctg cgtctagtgt tccagctgtg actaagtttg ctaaaactgc ggcaatccct
     2761 gccacgatcc cagtgccgac aaatgcaggg atcattggta caaagatatt agagatgtct
     2821 tttaataccg atttgatttt agagggtttt tgtttcgatt tttgtgccgc tttcatttct
     2881 tgtgcccgtt cgtttacacg gtcttttcca gatgcagaag aacttgctgt agttgcacta
     2941 gcagaaggaa gtttttctcc taatttcacg cctgctaagt caaccatttc ctgcgctact
     3001 ttgttcactt tcccaggtcc gatgatgact tggagctgtt catcctctac tacgccaagt
     3061 actcctggaa tcgcttttaa ttccgagaca tttacaagat catcattgat gaccttcata
     3121 cgtacacgag tcatgcagtg gacaatgctt tcaacattgc ccataccgcc tgctccttga
     3181 taaatttgtt ttgccagttc ttccaaagtc aattcgtttt ttgccatggt cttttccccc
     3241 tcataaggtt ccttttacaa aaccgtttgc ttcagctaat tttgcttgag ctgcctcttt
     3301 atcactattt gtcaaaatca tgacgatagc cagtttgaca ttttgatctg cttcctcaaa
     3361 gtacttttca gctgtttcgt agtcagcttc tgtcgcttgc atgatgattc gttttgagcg
     3421 ttcaattagt ttttcatttg ttggtttgac gtcgaccatc aagttattga atactttacc
     3481 gataccgatc atactgatag tagaaatcat attcaaaatc agtttttgtg ctgttcctga
     3541 cttcaatcga gtagagcctg tcaaaaattc tggtcctgca tcgacttcga ttgggaaatc
     3601 cgcatgctcg ctgatttctg cttgtttatt gcaagaaatc gttcctgtaa cagcaccaat
     3661 cgattttgcg taatctaatc caccaatcac atatggtgta cgaccgctag ctgcgatacc
     3721 aataactata tcattttttg ttaaattcag cttgattaga tcttcttgcc ctaattcata
     3781 agaatcttcg gctccttcga cggctacagt cattgcttct tctccaccag caattaggcc
     3841 tatgaccatt tccggttcta caccgaatgt cggtacacat tcagcagcat ctaatacacc
     3901 aagtctacca ctcgttccag ctcccatata aatcaagcga ccaccttttt taaaggattc
     3961 aatcgtgttg gcgatcactg gctcgatact tggcagttct ttttcaacag cagcggccac
     4021 tttctgatct tctttgttca tgagttggac tgcttgagaa atactcattt catctaatcc
     4081 aaaagtttct tggtttctgc gttccgttgt taggttctct aaattgattt tgctcatttt
     4141 tatcttcctt cttttcttat aaattgatag ttcataccag ctttgatttg gttgatcagc
     4201 ggtagttctt ctttgatgac tttggctact acattcactt tttcatccgc tggcaaatca
     4261 tatttagtta attggatctc acccatatag cgcaaatact tttcgttatc taaagtaata
     4321 gatcctttca tacgcttagc tgtatttctt actgggattg gcggaatctc tcgaaaacgc
     4381 gcatctgcgc ttcgtatgac atctcttgca tcatcttgcc gattcgtatg ttttcctaat
     4441 atatattcgt aaaaaacttc atctacaggt tccgtatgga gcaagactgt ctgttctttt
     4501 tggaaagaag caaattgctc ctgaacgtgt tctgacagac ctgcatctcc gatataaaca
     4561 atatccgtgt tacttgctaa tagatcaaga gcacctgcca gaggatggat ccctcgatgt
     4621 tcttccaaag ttggtaatcc ttgatacagt ggacctctca actctgtatc tcctggtaca
     4681 aagccttgta caaggaatcc ttgagttttg agccattgat ttttttcctg ataccattct
     4741 ttgtccaacc ctgtttcaag acgtggataa taattatgcc aagcttcaat ctgtgagaaa
     4801 tcagccgctg cttctttcaa ctcatcgata tcattaggcg tgatcgtgct ggcattcaaa
     4861 ctgattttca attgatgtga ccagtcggcg atctgctgat tagaaatatg ataatccatt
     4921 cttaaccctg ttacaccaat tgctttcaag ggtgttagat cttctaatga aaagccagct
     4981 cgtttcaatg cctctcctga aatatcgacc atcaactcta gtttattctt tttcgcttcg
     5041 cttcctagtt cgatcaatcg attttgataa gccgaaacat catcttccgg tatatgcatg
     5101 gaagtgaaaa taccgacaaa gccattggat gccatttggc ggatatactg tttcttctct
     5161 tctgacaaag aatcattcat aaatattgaa aagccaaaca tattatccct ctttttctgt
     5221 atccgtcttc atttctatga cta
//
LOCUS       DAHHZJ010000170         5218 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.172, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000170 DAHHZJ010000000
VERSION     DAHHZJ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.172"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            47..835
                     /locus_tag="NBL02_002495"
     CDS             47..835
                     /locus_tag="NBL02_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCD2573134.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(902..1570)
                     /locus_tag="NBL02_002496"
     CDS             complement(902..1570)
                     /locus_tag="NBL02_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HCD2573135.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            1685..3397
                     /locus_tag="NBL02_002497"
     CDS             1685..3397
                     /locus_tag="NBL02_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2573136.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            3400..5172
                     /locus_tag="NBL02_002498"
     CDS             3400..5172
                     /locus_tag="NBL02_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2573137.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         1641 a          899 c         1115 g         1563 t
ORIGIN      
        1 atttaaagtt attcaaaaat aacttcttac taaggaaggc gaaaatatgg catttattca
       61 agcaaatatt tattcgaatg tgttagaaat ggaagtcaat ctaaatgtga tactaccgca
      121 aacaacaaaa aaggtgattg gcaccagttc agagcaagag acaacggata tacctgtttt
      181 atatttactt catggaatgg gtggcaatca tagtgtatgg gaaagacgta cgtcgattga
      241 acgttatgta gcaaactacg gattagctgt catcatgcca tctactgatc ttggctggta
      301 cacagataca gtttatgaca tgaagtattg gacattcatt gcagaagagc tacctgagat
      361 ttgtcatgaa ttattcccgc aattaacaag aaaaagagaa aaaacatatg ccgctggatt
      421 atcgatgggc ggatatggcg cgttaaaact aggccttgca aaatcagaaa atttcgcagc
      481 agtaatctct ctttcaggcg ctgtttctgt aggaaatcga atggatgatc ttttgatggt
      541 tcgtaaagca tctttttggg aagggatctt tggaccattg gatcaaatcg aaggttcaaa
      601 aaatgatccg caatacttat tgaaagaact agtaaaaagc ggaaaaccag ttccaagatt
      661 ttatatggct tgtggggaat ctgattttct ttatcaggca aatcaggaaa tggtatctgc
      721 tatgcaggaa caaggcttgg cggtcacctt tgaacaagga cctggcgatc ataactggat
      781 cttctgggac caatggattc aacgtgcatt ggcgtggcta atggaagaaa aataaaaaaa
      841 gagtcttaaa aagatagctc ctatagagat cacaaacata gtgatctcta taggagcttt
      901 tttactgctc gttttctctt cttttatttt gttggcataa gccttcaata taggtttgtg
      961 cactcgttag aaagagtgct ttttcattca attctgcatg ggactgcata ctttctttaa
     1021 atccctctaa ttgattttca actacataat tacgcataaa tagaactgtt tctttcaaaa
     1081 attcatttcc gtccataatt ttttgtcgaa gctgctgttc atgctgctga tccattaact
     1141 gtccaatcaa taaattgtgg agagaggcaa tggcctgatg actttgggtg actgaaaatc
     1201 cttcacgaac aagtaattgg atcatttgct ccaaatgttc taatcgactc ggaaaagcgg
     1261 gcacagtatt gatttctaat tctgccccac aagggaaacg tgtataaagg tcatagtaat
     1321 tttccatata ctgcagcaac tgttcattcc aaggcaaagt tggatctgga agcgctaatt
     1381 cttcttccat tgtttcagcc attttttgca agagctgttg tttgtttttg aaataccaat
     1441 ataaagcagg tgcttgaaca tgaattctgt ttgcgatgtt tcgcatggaa agctgcgaaa
     1501 gagcttgtgt ttcagccagg atatcaaagg cggcctgaat gatgattgct tgagaaagct
     1561 ttatttccat tttatcaacc tcgtgtattg cattatcttt ttaaacatgg taaattgtta
     1621 gtcggtctaa ttttacagtg ttaaattagt cctgtcaatt taaacaaagg ggtgtaatca
     1681 acgaatgaaa ttaatcttac gacatgcgaa aaaatataaa gtagctgttt ttatttcctt
     1741 gctgtccgta gcagtcatgg ttgcagcagc attatggcaa cctaagttac tacagcaagt
     1801 attggaagct attattacag aagataatga tgaaatgaaa acaattggta tttacctgat
     1861 cagtctggct ttgctaggat tagccgcagg tgtaacgaac acgattttct ctgcaaaagt
     1921 agctcaaggc gtaagtgcag atatccgaga agaagcattt cgaaagatcc agacattttc
     1981 atttggaaat atcgaacagt tctctgctgg aaatcttgtt gttcgtctaa caaatgatat
     2041 cacgcaaatc caaaatttag tgatgatttc tttgcaatca ctttttcgaa ttccattctt
     2101 atttattggt gccttcattt tagctatgat cacgatgccg caattatggt ggatcatcgt
     2161 agcgcttatc gttacggtat ttctgattac ggcactttca tttacaagga tgggcaagca
     2221 cttcatgatc attcaaaaac tgattgatcg tgtgaatagc attgcaaaag aaaacttact
     2281 agggattcgt gtagtgaaat cttttgtaca agaagaaaac caattgaagt cattcagcaa
     2341 agtaagtgag gacttaacaa aacacaacat catagtcggt acattatttt ctgtgatgat
     2401 cccgtccttc atgctggctg ccaatttggc tgtggttggg gcgatctttt ttgtgagcga
     2461 tcttgttaaa gatgatccta cgttaattgg tggaattgct tcattcatga actacttgat
     2521 gcagattatg atggcaatca ttattggcgg aatgatgatg atgatgactt caagagcagc
     2581 cgtatcgctg aagcggattg cggaaatttt agatgctgaa ccagatctta cttatttaga
     2641 cgttccagaa caagagttga cgggttctgt cacttttgac catgtttctt ttcgttatcc
     2701 tggtgaggat acagatacgc ttaaagatat cagcttttct atcaaacctg gagaaatggt
     2761 tggtatcgtc ggggcgactg gtgccgggaa gtcaacacta gcacaattga tccctcgtct
     2821 atttgatccg acagaaggca aagttgaagt aggtggtgta gatcttcgtc aagtaaatga
     2881 aaagagttta agacaaacgg tttcctttgt tttgcaaaaa gcaattttat tctcaggaac
     2941 gattgctcaa aacttgcgcc aagggaaaaa gaatgcaaca gaaaaagaga tggaacatgc
     3001 ttcttctatt gctcaagcaa aagaattcat cgaaaagtta tctgatcgtt acgaagcacc
     3061 aatagaagaa cgtagcagta acttttctgg tggtcaaaag caacgtttat ccattactcg
     3121 tggcgttatc ggtaatccaa agatcttgat cctagatgac agtacgagtg cactggatgc
     3181 tcgctctgaa aagctggtcc gagaagcact cgatcgagat ttgaagggaa caacgacgat
     3241 tgtcatcgca caaaaaattg cttctgttgt caaagcagac cgcatcttag tattggatga
     3301 aggcagatta gtcggcgaag gaacccatga ggaattggta gctggaaacc gtgtctatca
     3361 ggaaatcttt gaaactcaaa aaggaacgga ggagtaagta tgactgatct aataaaagca
     3421 agtaaatttt tctatcacta tttgaagcgg tacaaactct cctttttctt tatctttatc
     3481 acagtgatca ttgctaccta tctacaagta aaggcacctc aatacatcgg ggaagctttt
     3541 caagaattag ctaattacgt aggaggcttg atgcaaggcg tagacgataa aagcaaattt
     3601 gtttctgtaa tatggaaact cttgctgttt tatgtactag ctagtgcagc caactttatc
     3661 tatagtattt tgttcacaca agtagtaggg aagtcaacga accgaatgcg tatcggtcta
     3721 ttcaacaaat tggaaaaatt gacgatccgt ttctttgatt ctcatcaaga cggtgaaatt
     3781 ttaagtcgtt ttaccagtga cttagataat attcaaaata gtttgaacca agctttgctt
     3841 caagtcatca cgaatgctgt tttacttgtc gggatcttga tcatgatgtt ccgccaaaat
     3901 gtagagttag cttgggctac aattgcttcc actccagtag ccgttttgat agctgtcttg
     3961 attatccgca aagcacggaa atatgtggat cttcagcaag atgaagtggg gaaactaaat
     4021 ggttatatgg atgaaaaaat cagcggacaa cgtgtcatca tcacaaatgg cttacaagaa
     4081 gaaacgattg aaggattctt agagcataat caaagtgttc gcgaagcaac cttcaaagga
     4141 caagcttatt ctggtctact atttccgatg atgcaaggaa tgtcactggt caatacagcg
     4201 atagttatct ttttcggcgg ctggttagca ttaaatggtg atttggaacg ttcggtggca
     4261 ttaggcttag ttgtgacatt cgtccagtat tcacaacaat attaccaacc actgatgcag
     4321 atttcatctg gatacagcat gatccaatta gcgattacag gggcaagacg attgaatgaa
     4381 atatttgatg aaaaagatga agtcaaacca gaaaacggta aagcatttga tggtgttgaa
     4441 aaaggtcttg cgttgcagca cgtagatttt ggttattctc cagaaaaact agtcttgaaa
     4501 gatgtctcga tcaatgtgga caaaggagaa atggttgctc ttgttggtcc aacaggatcg
     4561 gggaaaacga cgatcatgaa tctgctaaac cgtttttacg atgtcaatga tggttcagta
     4621 gtcatcgatg gaatagatat ccgagattat gatcttgatg cattacgtag acatgtagga
     4681 atcgttttgc aagaatctgt attgttctcc ggaacgatca aagacaatat cacattcggc
     4741 aaaccggacg cttcagatga agaagtcatc aatgcagcaa aacaagcgaa tattcatgac
     4801 ttcatcatga cgttagaaaa aggttatgat acagagatat ccgaagaaaa taacttattc
     4861 agtacaggtc aaaagcagtt gatcagtatt gctcgaacga tcatcacaaa tccatcttta
     4921 ttgattttgg atgaagcaac aagtaatgta gacacggtaa cagaagcaag gatccaaaaa
     4981 gcaatggatg aagcaatcaa aggaagaacc agctttgtga ttgctcaccg gctgaaaaca
     5041 atcttgaatg cagatagaat cgttgtcttg cgtgatgggg aagtcattga agaaggcagt
     5101 catcatgagt tattagcaga aaatggattt tatgcggaac tttatcataa tcaatttgta
     5161 tttgaaagtt aaattaaaag aacctcgtta taacggggtt ctttttatat attttatg
//
LOCUS       DAHHZJ010000171         5201 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.173, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000171 DAHHZJ010000000
VERSION     DAHHZJ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.173"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            68..1186
                     /locus_tag="NBL02_002499"
     CDS             68..1186
                     /locus_tag="NBL02_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCD2573138.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1314..2690
                     /locus_tag="NBL02_002500"
     CDS             1314..2690
                     /locus_tag="NBL02_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCD2573139.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3091..4671
                     /locus_tag="NBL02_002501"
     CDS             3091..4671
                     /locus_tag="NBL02_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCD2573140.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            4869..5093
                     /locus_tag="NBL02_002502"
     CDS             4869..5093
                     /locus_tag="NBL02_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HCD2573141.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
BASE COUNT         1769 a          837 c         1076 g         1519 t
ORIGIN      
        1 aaaaaaagaa taaaagaatt tttaacgaat ataatatata ctagtaagaa agaaaggagg
       61 aaatctattg tttgaaaaac ttagacagtc taaactaatg ttttggtcgc ttgaattatt
      121 gatcatagct gcattgatca tgatttcatc agaaataaac tttattttcg aaccgatcgg
      181 gacatttttt tccacgttat ttgcacctgt actgattgca ggctttcttt actatttatt
      241 gaatcctatt gtcaagctgt tgatgaaagc aaaaatcaaa agaatatggg ctgtcgcaat
      301 tgtgctgctc cttttagttg cagcaattat ttggatttta cttagtgtca tcccaagtct
      361 agtacagcaa atttcctcat tggcatctaa tatgcctgat ttcatcaaac aggtagaaag
      421 ctggttaaaa gaggtagccg aattgccttt gttcaaggaa gtagatatca ataaatattt
      481 tgagcaatta gacatttctt acgggacaat cattcagcaa ttccttagtg gagtatccaa
      541 tagtttaggt tcgatcgttg gaacgatcgc ttcggcaacc gtagtaatca ttacagcacc
      601 attcatcttg ttctatatgt tgaaagacgg agaaaaacta gtgccaaaca tccaacagtt
      661 tttcccagaa aaaagaagaa accaaattgt ggagttattg gggcaactga ataaaacatt
      721 ggctaattac attagcggac aagcaatcga atgtttattc gttggtacct tcacattttt
      781 aggatatatg gtaattggtg tggattatgc tttcttattt ggggtcattg cgggattgac
      841 aaacctaatt ccatatttag gaccttatct aggactagca cctgcatttc tagtaacagt
      901 cttcaatgat ccggtaaaag cattactttg ttgtgtggtt gtgctaatcg tccaacagtt
      961 ggatgggaat atcatttatc ctaatgtaat tgggaaatca ctgaaaatcc atccattaac
     1021 gatcattatc gttcttctag tagctggaaa tattgccgga ttgcttggta tcttcttagg
     1081 tgttcctttt tacgcaatct gcaagacgat catttcctat ataatcaaaa tcgtcaaaga
     1141 agacaagcaa aatgaaaaca agaagaaaat tgctacgacc tcgtaggtag ttttagtaca
     1201 cttgttttta ttgcaaagaa aattgctttg tgataccatt ttaacgtggc tatagtgcca
     1261 cgtttttttc tatcgtaaac taaaaattaa ttttataagg agagaatgcg attatgaatg
     1321 ctgaccctga gagtcagtcg ctgttagcgc agattttatt attgatcgtt ttgacattga
     1381 tcaatgcttt tttagcagct tccgaaattg ctgttgtttc gatcaataag aatcgaatcg
     1441 agcaaaaagc tgatgaagga gatgtgaaat ctcaaaagct tttaaaaata ttgcagaatc
     1501 caaataattt cctatcgaca atccaagtag gaatcacttt ggtcaacatt ttgtctggtg
     1561 cttcactagc taatacttta tcggaacggt tagctccggt attaggtggc ggcacagcgg
     1621 caaaaaatat tgcaaatatc attgtgctgg ctattttgac ctatgtatcg attgtttttg
     1681 gagaattgta ccctaaacgt attgctttaa ataaatcaga agaagtcgca aagttcactt
     1741 cagggctgat acgcttgatt ggtgtagtag ctaagccctt cgtctggctg ctttctgctt
     1801 ccacaagttt attggcgcgt atcacgccga tgacttttga tgatgaagat tcaaaaatga
     1861 ccagagatga aatgcgttat atgcttgaaa acgaaggcgt attgaataat gaagaattag
     1921 aaatgctcca aggggttttt tctcttgata cgaaagttgc ccgagaagtc atggtcccac
     1981 gaacggatgc atttatgatt gatatcaacg acactgtgga agaaaatgtg aatgaagttt
     2041 tatccgaaaa ctattcaaga atccctgttt ataacgaaga caaagataaa gtagtcggaa
     2101 tccttcatac aaagaattta ctaaaagctg cccataaatt cggctttgat aatttagata
     2161 tcaagaaaat catgcaggag cctttattcg ttcctgaaac ggtctttatt gatgatctgt
     2221 tatatgaaat gaaaaagacg cagaatcaga tggctatttt attagatgaa tatggcggcg
     2281 tcgttgggtt agcaacacta gaagaccttt tagaagaaat cgtcggagaa atcgatgatg
     2341 aatcagatga agttgagaat ctttatgaaa aaatcgatga acacgaatat atcatccaag
     2401 gacgtatgct gattgatgag ttcaatgaag cttttgacag tgatctgcat atgagtgatg
     2461 tagatacgat ggctggttat ttgattacag cattaggcat gattccggat gaaggcgaaa
     2521 agttatcgtt tgatgtcgat aatattacgc tggtttctga agaaatggaa ggttctcgaa
     2581 tccttaaaat ccgtgtcatt tttcatgatc cggaagaaac agaagcggaa cctgaagaag
     2641 aacgaagata cttcagaaaa gaattcgaag atgacgagcc aagaagataa ttggaaactt
     2701 ggttatgaaa gacgtgttgc ttgaataaac aagcaacacg tcttttcttt tagtcagctg
     2761 acaaaaaaat atttgactct tatggtttgg tggctttttc attcgctgaa tccatgctat
     2821 actgattcag ttgtagtagt tgacaaaggt tgtgaaaaag ctatcctgca ttaagtaata
     2881 agagcagcca agcaaaaaca gcttttcgta tttttggctt gggtgggctt attgctgata
     2941 ggctgtctct agaacgccgt ttactcggaa aaagagactg ctacacggct tttgtcacag
     3001 ccctatatga aattgtcttt ctttaagaga tcggacaatc tgtgcctaga gaaaatataa
     3061 ataattgaat ggaagaaatg gagttaggtt atgaataatc caaaattaaa gcaacaagtg
     3121 gacaatcgca gaacttttgc gatcatttcc catccggatg ctgggaaaac aacgattaca
     3181 gaacaattac tattgtttgg tggggctatc cgtcaagccg ggaccgtaaa aggcaaaaaa
     3241 acaggtaatt ttgccaaatc agactggatg gaaatcgaga aacaacgtgg gatttctgtt
     3301 actagttctg ttatgcagtt tgattatcaa gggaaacgag taaatatctt ggatacacca
     3361 ggacatgaag atttttctga agatacttat cgtactttga tggccgtgga tagtgcggtc
     3421 atggtaatcg atagtgcaaa agggatcgaa gcacagacaa agaaattatt ccaagttgta
     3481 aaaaaacgtg gcatcccgat atttactttt atcaacaaac tagatcgtga tggtcgtgaa
     3541 ccattggaat tgttagaaga attagaagag ctattagata tcgaatctta tccgatgaat
     3601 tggccaatcg gtatgggtaa agggctagaa ggtctttatg atatctacca taatcgtgta
     3661 gagttttatc gtccggaaaa ttatcaggat gaacgattag tagaattgga tgaagatgga
     3721 ctgcttcccg aaaatcatcc tctaactgaa aattccttat atgaacaagt cttagaagaa
     3781 gtagaactaa tcaaagaggc tggagatgca tttaatcaag aaaaaattgc tcgtggcgat
     3841 cagacaccag tattttttgg ttctgcattg actaacttcg gtgtacagac ttttttagag
     3901 actttcgttg aattggcacc agctccttat ggacacaaaa cacaagatga tcagatggta
     3961 agtccttatg aggaagaatt ttcaggtttc gtattcaaga tccaagcaaa tatgaatcca
     4021 gcccatcgtg accgaatcgc ttttgtccga atctgttctg gaacattcga acgaggaatg
     4081 gatgtctggt tggaacgtac aaataaaaaa ctgaaattaa gcaatgtcac acaatttatg
     4141 gcagattcga gagaaaacgt cgaaaaagca gtagcaggcg atatcatcgg tgtatatgat
     4201 acaggcaatt atcagattgg ggatacattg ttcgaaggaa aactaaaagt tgcttatgaa
     4261 gaactccctt catttactcc agagctgttt atgaaagtga ctgcaaaaaa tgtcatgaaa
     4321 caaaaatcct tccacaaagg aatctatcaa ttagttcaag aaggagcgat tcagctttat
     4381 aaaacatatc taacggaaga atatatcatt ggagctgttg gtcagctaca attcgaagta
     4441 ttccaatatc gtatgttaaa tgagtataac gccgaagtaa tcatgtctcc aatgggacac
     4501 aaaatcgctc gctggatcga tccagaagat ttggatgaga agatgagttc aagtcgcaat
     4561 atcttggctc gtgaccgttt cgaccaaccg ttgttcttat tcgagaacca atttgcagaa
     4621 cgctggtttg cggataaata tcccaatgta aaattaaaaa gtttgatgta aagaagtctt
     4681 gacacaagct gccctgcatc aagtaataag aatagccaaa caaaaacagc ctctcatatt
     4741 ttgtttagag ccgtgacgga ttttccgtga cggctctttt tactatatta aaatcataga
     4801 aatgtctaat caaaaaaata taaaaattat tttgcgtaca tatgattaaa gaaaggagag
     4861 tgtgacacat ggaaacagaa tggcaaagac gtgaaagaaa aaaacagaaa aaagaagaga
     4921 tgttacgatt atgtcgatta ttagatgtaa aagactggga caaaaacgag caaattgcac
     4981 aaaaagtacg atcaatcatc cataaagagg aaaaagttaa aaataaagac acgcgtagaa
     5041 ctgaaaatga aaagagaaaa gtacaaaaga aaagagaaaa atcaaaaagc tagaaagaaa
     5101 aagaggctga gacagaagcg tttaactcca agaaataagc tggaattcac aaaaatttga
     5161 aaagcaattt tcgtgaattt cagcttattt ccgaaggagt t
//
LOCUS       DAHHZJ010000172         4945 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.175, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000172 DAHHZJ010000000
VERSION     DAHHZJ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.175"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            3..1010
                     /locus_tag="NBL02_002503"
     CDS             3..1010
                     /locus_tag="NBL02_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCD2573142.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1022..2026
                     /gene="neuB"
                     /locus_tag="NBL02_002504"
     CDS             1022..2026
                     /gene="neuB"
                     /locus_tag="NBL02_002504"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HCD2573143.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2023..3171
                     /gene="neuC"
                     /locus_tag="NBL02_002505"
     CDS             2023..3171
                     /gene="neuC"
                     /locus_tag="NBL02_002505"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HCD2573144.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            3144..3821
                     /locus_tag="NBL02_002506"
     CDS             3144..3821
                     /locus_tag="NBL02_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCD2573145.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            3811..>4945
                     /locus_tag="NBL02_002507"
     CDS             3811..>4945
                     /locus_tag="NBL02_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_066164118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCD2573146.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKI"
BASE COUNT         1835 a          647 c          972 g         1491 t
ORIGIN      
        1 aagtgtttaa aggaaaaaca atattagtaa ctggcggaac tggttccatt ggaagtgaaa
       61 ttgttcgtca gcttttaaaa tatgaaccaa aagctatcag aatttatagt agaggtgaag
      121 aaaaacaatt ttatatgcaa caagagctct caaatcacac taatttgaga tttttaattg
      181 gtgatatcag agactctcaa aggattgatt atgcatgtcg tgatgtggat attatttttc
      241 atgctgccgc aatgaaacac gttccagcaa gtgaatataa tcctatggaa gctgtaaaaa
      301 caaatgtgat gggaactcaa aatgtaatag atgcagcgat caaaaataat gtaaaacatt
      361 ttgtaggtat cagtacagat aaagtagtga gtcctactaa caccatgggt gcgacaaagt
      421 tattgagtga aaaattaata atagctgcca atcagtataa aggaaaaaca gaaacaatat
      481 tttcttgtgt aaggtttgga aatgtcatgg ggtcaagtgg atctgttatt cctcttttta
      541 gtaaacaaat aaaaaacaat aaagatatta ctttgacaga tgttgatatg acacgattta
      601 tgatgtcaat accggatgct gcaagattag ttctgtctgc tgctatgaat tcaaaatttg
      661 gagaaacata tgtcttaaaa atgccggcaa tcaatatttt aactttagct caatcattaa
      721 taaaaatata taatgataca catgatgaaa aatatagagg tgaaataaaa gttgtaggta
      781 tcaggccagg agaaaaatta tatgaagagc taatgactac agacgaaatt gaacgctcat
      841 atgaaaatga taaaatgtat atcattccac cacactatca tgctactgac gaactgtaca
      901 aagattatga aaaaattaaa atcactgcat actcttcaga tgcaacagaa ttaatgagtg
      961 gacaggaatt tattagaatc gcaaaagaga ataatgtcgt gacttggtaa ggagttttat
     1021 gatgaaatca gaagtatatg taattgcaga aattggtgta aaccataatg gaagtctaga
     1081 tttagcttta gaaagtattg ataaagctaa agaatgtgga gctaacgcgg ttaaatttca
     1141 aacatttaaa acgaataagc tagtttccaa aaaggctcca atggcaaaat atcagaagga
     1201 taatcttaaa gaggacagtt ctcaatttga aatgctaaaa aaattagaat tatctcaatc
     1261 tgattttatt agaataaaag attattgtat acaaaataaa attgattttt tgtctactcc
     1321 ttttgatgaa gaaagtgcag actttctaaa acaaattggt gtacaaggat ttaaaattgg
     1381 ctcaggagat ttaacaaatt taccttttct tagaaaaata gatatgtatg ggttgccagt
     1441 tttattatct acaggtatgg ctgatattca tgaggtcagt gaggctgtag attgtttcat
     1501 agatagtcca gttactattt tacattgtac ttcaaattat ccggctagtt ctgaagatat
     1561 taatttattg gcgattaaca cattaaaaga aatgttcggg atacccgttg ggtactccga
     1621 tcattcattg ggatatgatg ttggtatttg tgctgttgcg atgggtgcta aggtaattga
     1681 gaaacatttt accttggata aggaactacc tggtccagat cataaagctt ctttagatcc
     1741 tcaggaattt gcagattttg taaagcatat tagaaacact gaaatatttt taggtgatgg
     1801 tgttaagcga gcaatgcctt ctgaacttag tacaaaagaa gtatcaagaa aaagcctagt
     1861 ggtcactgag aatttaaatg tgggagatat tcttacagag aaatctctta caataaaacg
     1921 tcctggaaat ggaatagctc ctaagttttt atatcaatat attggcaaaa aagttaaaag
     1981 accaataagt gcagatagtg ttctgcttga ggaagatgtt gaatgaaaaa aatacttata
     2041 gtaagtggaa cacgagctga ttatggaata tataaacctg ttgctgagaa tttactacaa
     2101 gataattggg atgtatcatt tgttgtttct ggaatgcatt tatcccataa atatggattt
     2161 actaaagaat tgatcttagc tgacggattt agaattagtg gagaagttca aagtttgttt
     2221 ttagagaaca ataagggaaa catggctcgg tctgtggcat tagagataaa tggtttcaca
     2281 aatatatttg aacaaactag ccctgatttt gtattattac taggggatag aggagaaatg
     2341 ttggcagctg cgatagcttg tttgtatctt ggaatcaaaa cagcacatat tcacggaggc
     2401 gaagtgtctg gtactgtaga tgaatcaata agacatgcca taacgaaaat agcatctgtt
     2461 cattttgctg ctacggaaaa aagcaaagag cgtttaataa aaatggggga agattcttgg
     2521 agagtttttc ccgtcggagc tcctagaata gatactattt taaataatag attacctagt
     2581 tttagagata taaaagaaaa atatcatcta aaatttgaaa agaaaaaata cgttttgcaa
     2641 gtatttcatc cagttgtaac cgaactagaa aatatagaaa aacaaatttt agctttgatc
     2701 aattcaatgg aacaacaatc tcaaccaatt atttgcattt tacctaatag tgatgctggt
     2761 agcgaaatta tcagaaaaat gtaccagaaa tatttaaaaa cggatattat ttatattacg
     2821 aatttagagc cggaggaata cctgactata ttaaaagagt gcaaatttat gatcggaaat
     2881 tcttcttctg ggataattga agctgcatca ttcaaaatac cagtaataaa tttaggaacc
     2941 agacaaaatg gacgagaaca atctaaaaat attataaatg caaatctaaa tgaaaatgaa
     3001 attgaaactg ctttgaataa aatacaagat cctcagttta taaaaagctt gaaaaatgtt
     3061 aagaacatct acggtgacgg caacagttct agaagaataa caaaaacatt gaatcaaata
     3121 ttgaatgatg gggaagatat taagtggatt caaaaacaaa tagcttatta aaatatacta
     3181 gtgatccata cattattatt ggggctggcg gacacgcaaa agtcattatt gaaaccttaa
     3241 gattagaaaa taagttaata tatggatttt ttgatgataa gcaaaataaa atgcaagatc
     3301 tgaaatatct tgggaaaata aacgaattga atagctgggc ggatagcaaa aaaaaaatct
     3361 ataaatttat tgtagctatt ggtgactgtg tagtaagaaa aaagattata aaaaaattga
     3421 atatgccaag tgagcaatat ggaatagctg ttcatccaag tgctatagtg gcaaaagaaa
     3481 tttcaattgg ttatggaact gttatttttg cgaatgctgt aattaaccca gatgctgtaa
     3541 ttggagagca tgctataata aacacaggta gcattgttga gcatgattgt agaattaata
     3601 actatgttca tttatctcca ggtgtatgct tagcgggtgg agtacatgta ggtgtaggaa
     3661 ctcaaatagg aattggttct caatgtatcc agatgaagaa aattggatct tggtgtatga
     3721 taggcgcagg gagcactatt gttaaggata ttccctcaca ttccttagct tatggcaatc
     3781 cagctaaaat taagaaagaa ggaattaatt ttgaattcta gaattttatt atcaagtcct
     3841 catatgggag gaacagaaga aaaatatgtt gctaaggctt ttaaagataa ttggatagcg
     3901 ccattaggtg aaaatgtgga taaatttgaa gaaagtatta caaaatatac tggttctaag
     3961 ggcggagctt ctgcgttaag ttcaggtaca gctgccatac atcttgcctt aagactgctt
     4021 aatgttggaa aaaacgatat tgtctttgct ccttcattta cttttatagc tacagtaaat
     4081 cctattttat atcaaggggc tgtaccagtg ttcattgact cagaacctaa tacatggaat
     4141 atgagccctg ttgcactaga gagcgcatta gaagaagctg aaagaaacaa ccaattacct
     4201 aaggcaatca tcgtagtgaa tttatatggt caaatggctg attatgaacg aattttagct
     4261 attgcaaacc aatttaatgt tccagtgata gaagatgcag cggaatcgtt aggttctact
     4321 tataagggaa tttcgtcagg cttatttggg acaattggga tctattcatt taacggtaat
     4381 aagatcatca caacctcagg tggaggagct ctagtgtcta gtgataaaaa atatgttgag
     4441 cattcaagat ttttagcaac acaagcaaga gatcaagcac cgtattatca acatagcgaa
     4501 ataggttata attaccgtct aagcaatatt tctgccggta tcggacgtgg acaaatggaa
     4561 gtattgaatg atagagtagc taaacgaaga gaaattttca atcgatataa ggaagctttt
     4621 tctggaattg atagaattga aatgatgcca gaagtggatg gcatggtgcc aaatcgttgg
     4681 ttgtctactt taacgatcaa tgattataat tctattgatc aagtagttaa cgtaataaat
     4741 aaactaaatg aaaaaaacat tgaaacaaga gctttgtgga aaccactaca tttgcaacca
     4801 gtatttcaag gggtaaaaat gtattaccat gataataatc ttagtgtgtc agaaaagtta
     4861 tttaatagcg gcttatgctt accttctgga agtaatatga cagatagtga acaagaattt
     4921 gttattgatg agtttaaaaa aatat
//
LOCUS       DAHHZJ010000173         4925 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.176, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000173 DAHHZJ010000000
VERSION     DAHHZJ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.176"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            140..280
                     /locus_tag="NBL02_002508"
     CDS             140..280
                     /locus_tag="NBL02_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573147.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
     gene            578..835
                     /locus_tag="NBL02_002509"
     CDS             578..835
                     /locus_tag="NBL02_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HCD2573148.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(806..1114)
                     /locus_tag="NBL02_002510"
     CDS             complement(806..1114)
                     /locus_tag="NBL02_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573149.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            1192..1965
                     /locus_tag="NBL02_002511"
     CDS             1192..1965
                     /locus_tag="NBL02_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HCD2573150.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIRK
                     HGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSST
                     DSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPKA
                     DGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            1980..2183
                     /locus_tag="NBL02_002512"
     CDS             1980..2183
                     /locus_tag="NBL02_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD2573151.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            2199..2519
                     /locus_tag="NBL02_002513"
     CDS             2199..2519
                     /locus_tag="NBL02_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HCD2573152.1"
                     /translation="MQYLEAKIPVPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRR"
     gene            2524..2694
                     /locus_tag="NBL02_002514"
     CDS             2524..2694
                     /locus_tag="NBL02_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573153.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEYRAKKPVGRD"
     gene            complement(2701..3483)
                     /locus_tag="NBL02_002515"
     CDS             complement(2701..3483)
                     /locus_tag="NBL02_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573154.1"
                     /translation="MDISDTPYYQKLIASSKLIGETIAPSLDVVNAMQPAIEQAKKLI
                     AIQDTFIASQAILNDSLIQIVEKRNKSIANMIPPSLFAIQKQLESPSFNIAKKMAATM
                     PKPYFSDISKITERFAVDMSKYDFRIGVATQELEKAFSSEPEVSTKNLLFKDTSNPSD
                     EFEAKFFDILNEQRKVLDDSIKLFEEINTAKNKDITNEPSEKQQASPAFYKDKMWYLE
                     QTGAALIGLVVTEIVNITIGVDPNNTISLALFLRYLLQFLIN"
     gene            3534..4241
                     /locus_tag="NBL02_002516"
     CDS             3534..4241
                     /locus_tag="NBL02_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HCD2573155.1"
                     /translation="MQELVILKNKEAVTTSLQVAETFRKEHRAVLKAIDDLKDGLAQK
                     YADLFWEDVYIHPQNKQAYRIVYMNRDGFTLAVLGFNNTQKVLEFKLKYIEAFNEMEK
                     AIRKNTAFDTIEDMMIYQLEEMKDVKKDVSMLKNTMRISGQQEFEIKQKGNTKVMEVL
                     GGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRM
                     EIDQLNRQQRLFGDENE"
     gene            4419..4760
                     /locus_tag="NBL02_002517"
     CDS             4419..4760
                     /locus_tag="NBL02_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573156.1"
                     /translation="MEKELSTLDQYLTDPSWGKSNIKETNNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKPEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            4753..>4925
                     /locus_tag="NBL02_002518"
     CDS             4753..>4925
                     /locus_tag="NBL02_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HCD2573157.1"
                     /translation="MSENKGTTTFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHY"
BASE COUNT         1837 a          765 c          935 g         1388 t
ORIGIN      
        1 aagaaagctt ttaaaatcaa tgtttatatg tcttatttaa ctatgttctg attttaacac
       61 tgtatgttaa tttagtcaac gcagtttttt aaaaagttgt taaatttgtt tacgaatata
      121 aaagaaagga gagaagaata tggaaaaaac agtctcggca aaaatcgaag atttgaaatt
      181 agacatactg aaacaagcaa aagtggcgat ggaacacgcg gtagataaag aagactctgc
      241 catggttgca gccatagcag agattttagc tcacgtttag tcatcgtttt tacggtcttc
      301 tggaagctga ccatagataa cagcataatg aatatctaaa taagcttgaa caatttcttt
      361 tggcgaaatt gattcgcctt tagtagatac ttgtgattcg tgatttagcc atgcaacaac
      421 taaatccgca gcgatttcaa acggcgttac actttgtgtc agttgccata aagaatttca
      481 caatatgtat ggtcgtggta ataataccaa agagcaattt gaggaattta tggaaattaa
      541 aaaatacaaa aaatcattta ttaaaggagg ctaaacaatg aacaaggaaa tgagccaaac
      601 aatcaaggta caaaaaatga tagatgatct aacacatgga attgatagcc aagccgataa
      661 aatcatcaag gaattgcaag gacaaaaagt taaggatgca aaaatgcttc tcaagactat
      721 caactttgaa atgaatccaa caaaaagaaa gctcgccgat gtgttggaag aaaagttggc
      781 ttccgctatc aacgagcaag aattactatt tgaaaccgat acttttaaca gctgatgttt
      841 tatagatgac agtttcatcc gaatctatat catcgccgag agaaaagaat gttgcttttg
      901 acaaaaagcc gataagacct acaagcggtc ttgatgttgc aagagtagat accccaatat
      961 caccttgcaa ggtattcacg taaatttgag aagaatagaa cattccatct ttttcttcga
     1021 tatttgacgc atcatcgtat ccaacgacca aagtatcggc aaatattttg attttttctc
     1081 catcaagcat agtaattaca aattctggtt tcataaattt tcacctcctt aacaattatt
     1141 tcagcctgtc acactgataa ggaaattata ccaaagaaag gaatgagaaa tatgaataca
     1201 ccacaaattt ttaatttcga gcaaaacgaa gttcgaacta ttttagtaaa tgatgaacca
     1261 tattttgtag gaaaagatgt agcagacgtt ctaggatatt cgaatccgca aaaagctatt
     1321 cgagatcatg tggacttgga ggataagacg cagaacgatt cgttcaccgt caatggaaca
     1381 gcagttgttt tgattaacga gtcaggcctt tacagtttaa tcttaaaatc aaaacttccc
     1441 tctgccaaaa aatttaaacg ttgggtaaca agtgaagtgt tgccagcaat tagaaaacat
     1501 ggaggttatc taactccaga aaaagtagaa gaagctttgc ttaatccaga tacaatcatt
     1561 caattagcaa ctcaactaaa agaagaaaga actggaagat taatcgcaga acaaaagatt
     1621 gcagagtacg aacctaaaat ctcctattta gatagcatat tatcttctac agattcagta
     1681 acaattagtc agattgcagc agattatggg atgtctccac aacagatgaa taaattactt
     1741 cataaactag gtattcagaa aaaagtcggt aaccaatggt tattgtgcaa aaaacacatg
     1801 aaccaaggat acacaaaatc tcatacaact gagatcccga aagccgatgg tggcactaaa
     1861 attgtaatga ataccaaatg gacacagaaa gggcgtctat ttatctacga attactaaaa
     1921 aaagaaggat attaccctca aatggattta gaggaaattg gttagaaagg agttttagta
     1981 tgactgacat tgcagaaatc actcaacgag atagagaaaa aatcaaagaa tatgtcgaaa
     2041 gttcaaagtt cttaacttac accatgctcg ctgaaagatt cggcattagc aaaagctact
     2101 tatctttaat tttaaacggt aaaaagactt ctgcagaagc aaatagaatc atagattcga
     2161 ttattactat gtacgaattg taaagggagg aacagctaat gcaatatcta gaagcgaaaa
     2221 tcccagttcc agaaggctat gtaattatct cccaagtgga ttatgaggag ttaaaaaaag
     2281 ctgatgatac tggtagatgg atgacgttgc cagaagtgct agaacggatt aacagaaaat
     2341 atgattggtt tacttctaga gttttaaaga acccaagata tagaaatatt atcgatatcg
     2401 aaaaaaacaa aaatggattt gtctattatc cagttgaagg aagagacaca tatctatttt
     2461 taagaagtaa aacacttgaa tttttagaaa caaatttttc ggaaatctta aggaggtaag
     2521 cggatgggta aatttaatag agcactagta ttcagcgcac cactaattat ctacgcttta
     2581 ggtctttggg gaagcagaca agcattaatc ggcacaattg tatatatggt ctggatcttt
     2641 atggggcttg atgaagctga atatagagcg aaaaagccag tcgggaggga ctgacttaac
     2701 ctaattaatt aaaaattgta acaaataacg caaaaataaa gctaatgaaa ttgtattgtt
     2761 aggatctact ccaatagtta tatttacaat ttcggttact actagtccta tcaaagctgc
     2821 cccagtttgc tctaaatacc acattttatc tttgtagaaa gcaggtgaag cttgttgttt
     2881 ttcagatggt tcattagtta tatctttgtt cttggctgta tttatttctt caaaaagctt
     2941 tatagaatca tcaaggactt tcctttgctc attaaggata tcaaaaaact tagcctcaaa
     3001 ctcatcagat ggattagaag tgtctttaaa aagtagattt tttgtactaa cttccggttc
     3061 ggaagaaaat gctttttcta attcttgggt agctacccct attctgaagt cgtacttaga
     3121 catgtcaacg gcaaatcttt ctgtgatttt agagatatca gaaaaataag gttttggcat
     3181 agtagctgcc atttttttag caatattaaa acttggagat tctaattgtt tctgtattgc
     3241 gaacaaagaa ggaggaatca tattagcaat agatttgtta cgtttctcga caatttgaat
     3301 gagagaatca ttgagtattg cttgcgaagc tataaaagta tcttgaattg caattaattt
     3361 tttagcttgt tctatcgcag gctgcatagc gttaacgaca tctagagaag gagcaatggt
     3421 ttcaccgatt aattttgaag aagctatcaa tttttgataa taaggagtat ctgaaatatc
     3481 caattatata caccaccttt ttacctaaat tataccaaaa gggagagaaa aaaatgcaag
     3541 aattagtaat tttgaaaaat aaagaagctg tgactacgag cttacaagtc gcagaaacat
     3601 tccgaaagga acatagagca gttttaaagg cgattgacga tctaaaagat gggcttgcac
     3661 aaaagtatgc agacctattt tgggaagatg tgtacatcca tccgcaaaac aaacaggctt
     3721 atcgaattgt ctatatgaat cgtgatggct ttactcttgc tgtattagga ttcaacaata
     3781 ctcaaaaagt tttagagttc aaattgaaat acattgaagc gttcaacgaa atggaaaagg
     3841 caattcgcaa aaatacagct tttgacacaa ttgaagacat gatgatctat caattagaag
     3901 aaatgaaaga tgttaaaaaa gatgtttcca tgcttaaaaa tactatgcga attagcggac
     3961 aacaagagtt tgaaattaag caaaaaggaa atacgaaagt tatggaagtt ctagggggaa
     4021 aagaaagccg agcttatgaa gaaatcagca aaaaagtatt ctcaaaattt tggtctgaat
     4081 ttaaacgtac cttttcaatc ccaagatatg gcgagttacc tcgtaagaga ttcgatgatg
     4141 ctgtttcatt tattgaaatg tggttaccag aaactgcgat tcgcatggaa atcgatcaac
     4201 tgaacagaca acaaagactt tttggtgatg aaaatgaata gagctgaagc gctaagaatc
     4261 gggacggtaa ttgctaatcg ctggtggaga cacaataaac caatcatcct aagccaacaa
     4321 catattgaca agcaaaaagc atggcaacaa ataaaaaagt gactccgccg gcaagcaaag
     4381 agtcacaaag aaaacacatc ataaggagat tttagcatat ggaaaaagaa ctttccactc
     4441 tagatcaata tttgactgat cctagttggg gcaaatcgaa tatcaaggaa acaaataatc
     4501 gaaaaatcag acgaaatctt ttgacggatg aagaactagc atgtgatcaa gacgatttgg
     4561 gaaattttgt gagtatttgg gatcatgtct atcttatcca tttatcgagg aagtccaaaa
     4621 aacctgaata tatctatgtc atcgaagatg gcttgattga tgcgctagaa gagtatgaca
     4681 gagataactt gattgatatc tcttattacg gatcaggtaa gaaatacatt gctgaaatgg
     4741 aggcagaatt tgatgagtga aaacaaaggg acaacgactt ttgaaaaact ttttagtcgc
     4801 aagttaaata aaatactcaa gaaaaaagga aattttgatt atttatcttg ggctcacgca
     4861 tgggagatta tgaaaaagaa tgatccacag gcaacggtaa ctattaatga gtataaacac
     4921 tacag
//
LOCUS       DAHHZJ010000174         4890 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.177, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000174 DAHHZJ010000000
VERSION     DAHHZJ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.177"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(406..780)
                     /locus_tag="NBL02_002519"
     CDS             complement(406..780)
                     /locus_tag="NBL02_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCD2573158.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(803..2203)
                     /locus_tag="NBL02_002520"
     CDS             complement(803..2203)
                     /locus_tag="NBL02_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HCD2573159.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            complement(2207..2617)
                     /locus_tag="NBL02_002521"
     CDS             complement(2207..2617)
                     /locus_tag="NBL02_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCD2573160.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(2635..3126)
                     /locus_tag="NBL02_002522"
     CDS             complement(2635..3126)
                     /locus_tag="NBL02_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCD2573161.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(3136..3927)
                     /locus_tag="NBL02_002523"
     CDS             complement(3136..3927)
                     /locus_tag="NBL02_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCD2573162.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(3920..4693)
                     /locus_tag="NBL02_002524"
     CDS             complement(3920..4693)
                     /locus_tag="NBL02_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCD2573163.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
BASE COUNT         1555 a          997 c          648 g         1690 t
ORIGIN      
        1 ctaggagtga gcatttttcg aaaaatacta aaaatttttc aaaataaaaa aaggcataca
       61 gacaataaac tgtctatacg cctcacagac tctattctat acatctaatt ttggctgtcc
      121 tgcaaaaaat atgtgttagc ctccatcgaa agatcaaact ttggaaaaca tcattataac
      181 tttgcttggt aagagctttg ctaaaacatg atatgacttg tatacaaaat gtggagtgta
      241 ttccaataaa taggaaggat tacaaaattt gaaaccaaat aagtaaaaaa tactgtcact
      301 ttgaattaat ttgaaatagt aaattctcat taaactattg aaatgctgat accaaaaaat
      361 gaagtacttt tcgttcatac caacttagag cacttcgtta tctatttatt tcttcatttg
      421 gcttatctct ataatctcat gatttggtcc gtagaaatta aagtaacgga ttccattttc
      481 ccagaacggt aattccataa tatttttatc taataattca tatcctaaat cttgaattga
      541 attgaatacc tcatctatgt catccgtatc taatgctaga tgatcaatgg ctcctctaca
      601 ctcagtagtt ttatcttccc atatttctat tatcgttcca ttcaactcga aaaagccgac
      661 tttacttccc acattcattt cttcattaat cagactaaac cctaaggatt cataaaagat
      721 ttttgaagct ccaaaatctt ttgttggaat tccaatatgt tgtaaaccat ttaatttcac
      781 ttacatcatc ccttcaattg ttttaaaatc cacattcata atttattgaa tagtctagtt
      841 tatctacctt gattgctccc tccgtaacag tcatacttaa tgctgttccg actttatcat
      901 acattctagt acttaaagca acattatcaa tatataaaac agctatcgta tcatcaacaa
      961 ttaactttag ttcatattct ttgcctattt ccaaaaatag tggtcgttgc agccccttat
     1021 caaacatttg aggccaagga tagttcggag tttttccgaa agtaagctga ttattaatca
     1081 atgaaatacg aaattcgtat ccttcttgag tatcactatc tacaaataat tttaaagcaa
     1141 actcctttgt taacgatgta atagtcaaag ttgtttcaaa tagaaaataa tcttcgattt
     1201 taggaacaat tactctttct ttttttccaa attctttctc taacttaaaa cttgaaaatg
     1261 gagtgatttc tttaaaattc tcaattattt tttcaggcag tttaacacca agagttttgt
     1321 ctgtcttttg aataatttga tgtggaacaa gtgcaccgcc ccaatcccag ttattttgat
     1381 cgttctcccc ttcttttgtt gccacccagc cagttagaaa acggccagtt tcattagttc
     1441 cgactgttct tgctgcataa taggcttttc cgtcgaaagc ttcgtctact ggtgatttcc
     1501 aatctgaaaa taatcctgct ccgactcgat atttagtctt tttatcttca caatactctg
     1561 aaaaaagtaa ataccacgtt ccatcaattt caaatatatc aggcatttca atcatgttaa
     1621 attcattatt agcccagtag tctcctttga aattccaatc ttttaaatct tcggattcaa
     1681 aatagaccaa tctccccgtc ggctttttct tacttgctgg ttttctagct cctagtatca
     1741 aaacgtattt tccatgatct tgatcaaaaa ttgcaaaagg atcgcgccaa tcattcttat
     1801 catatccttc ttgaggaaca agttttgctg cttcgccatc ctttttccaa tgaattaaat
     1861 ctttagaact tgcaatcatt aacacttctg aagtttttcc atccaactta gctttgcgat
     1921 tatgtccagt atacaaagct aaatactcat tgattccttg gacaatactt ccagcgtaaa
     1981 tgtattgatc aatgtcatcg ttgccaccac gatgaattac ttccccgtat tcttcaaatt
     2041 tcacaaaatc agtggttgta actagtgacc acccaaaagg atcagttaaa ggtttaggat
     2101 ttctcgtatc tcgttgatga aatagataaa aagttccatc tttataaaaa ggcatgcaat
     2161 ccccaaacca aaaatcttta ggcttgtaaa aaattttttg catttatcac tcatcctctt
     2221 cgttagtatg tctttgcata tctgcaagac acgcaattgt attcatattt cttagatttt
     2281 cgatgatatc atcgcttttt tttccttgta atctcaaatt gacagcctca atcaaaattc
     2341 ctaaattcat gccaccaaga agttgaaacc cattttgaaa tacttcactt cttttagcaa
     2401 attcgttgaa aggtgttcca ccaatcaagt cagcaaatac cataacttct tgatttttta
     2461 cttgagaaac tattttttca atgttttcac ggaattcttc aatgcctgca tctccaagtg
     2521 ttaccacatg acaatttaaa ttatcgccaa aaaacatttt taatgtatct acaaatccaa
     2581 tggcaatatt tccatgagat gtaattataa actccatcca ctcatcctct gttcttaata
     2641 aataaaatcc tttaaaaatt cttttgaatc tctaggagtt tgtatcactg tcacttcaac
     2701 tccatcacta attagttctt taaaagcctc tctctcttct tcagtaatat ttatcgtcgg
     2761 acggatgcgt tctgttccat tccttcttga taaatttcct acgtttacac ttgttattgg
     2821 taaaccgtta tcaatcaaaa atttaacatc gattggagaa actgcaacaa taagaacttt
     2881 ttgtccttca tatttaccag aaagaatatt tttaactgct acttcccttg taattattga
     2941 agttctaaca ttaggtggtg cagcgaggcg aactacagct ttttttacgt catcattggc
     3001 tacttcatta ttaataacca taattctgtt agcactcaat tgatttgtcc attgtgttgc
     3061 tacttgtcca tgaatcattc gatcatcaat acgaacatgt ataatacctt tcattttttc
     3121 atccatattc aacacctact tcaaaatatt taacgctctt aacgcaatag aaactactaa
     3181 gattaataga attacataaa ctggtttaac ctttcgtcct aacaaataat acacaattgc
     3241 tactacacat gcaggtaata aaccaggcat aacttgatca aagatttcat ttccggtcat
     3301 tgttaagtcg ccttgtttaa attcaaagcc aacattcact tttacaactg tggcaatgag
     3361 tgcaccgacc accattaacc ctaaaattga tactgcatca gtaatagact ttaattttgt
     3421 ttcaatttga gttagtaggc tcgcacccga ttctttccca gcaaggtata aaggataagc
     3481 agtaatcctt aataaaattg atgccagtat ccacaaaata attccaacaa aactaccttc
     3541 caatgccata ttagctgcaa ttgctccaaa aatagtccat ggaataacta caaatacact
     3601 atctccaatt cccgctaaag gacccattag acctgttttc agagcactta ctgcttccaa
     3661 agacttattt ttttcttctt tttcaattcc aacattcata ccaataatgt aaggcgctgt
     3721 atacggcgta gtgttaaaaa attgtaggtg agctaaaacg gattgtttca ttccttcagg
     3781 atcatcttta tatgtgtcct tgataaaagg aagcatagaa tacgcataac ccaacccttc
     3841 cattttttca taatttaatg aagttgcacc attgagaatc caccggaaaa ttacttgttt
     3901 gtcgattttt gtcagtttct tattcatctt cgtcaaaacc tcctgttgca gctggtgcta
     3961 cttgagtatt atttgatcta tttttatata cgattagtgc gattgcagcg ccaagtaacg
     4021 aaatccctaa aattggtatt tggaaataaa ctgccaaaac gaaaccaact aacaaatagg
     4081 caaagtaatc ttttgtaggt aaatatttaa gtagcattcc tatcccaaca gcaggtaata
     4141 gtccccctgc tacctttaaa ccaccggtaa cccattcagg aatataattg attgcacttc
     4201 ccactaacgt tgggcctaat atcacagtta acaaaacagg aattccagat acaaagcttg
     4261 tattaattga tgccaaatat tgcatccact ctaatttttg ataatcttct ttttgtgcga
     4321 atttttctgc cttttgttga aaaaaaatgt ttgttgacca ctttagaaca tctagttgta
     4381 caattaacat tgctaccggt attgctaaag ttattcctat agaagcatcc tggttagttg
     4441 agattgctaa ataagtaccg atcaatgctg ctgtttgata atccggtact gatgcaccac
     4501 caaaactagt aatacctagt gtcattaatt gcaaagtccc accgatataa agtccagttt
     4561 gaatatctcc cataataagt ccggtcaaga aacctgcaac gacaggttgg gaggttccga
     4621 ttttgggccc ttccttatcc cagttaatga aaaatccata caacacaact aaaaatgttt
     4681 gccaaaacgc cattttgttc tctcctttct tcttatactt ttatataagc aattactgtg
     4741 ccaactaaca ttatccaata aagcgggaaa atacagtaaa tattaattaa atgagcgtct
     4801 aaatatactt tggacaccct ttcatttttt tagacgctag tatgctattt agaaaatagt
     4861 ctgctcaaaa attgtataga ttaactcata
//
LOCUS       DAHHZJ010000175         4849 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.178, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000175 DAHHZJ010000000
VERSION     DAHHZJ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.178"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            124..924
                     /locus_tag="NBL02_002525"
     CDS             124..924
                     /locus_tag="NBL02_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCD2573164.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            943..2811
                     /locus_tag="NBL02_002526"
     CDS             943..2811
                     /locus_tag="NBL02_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCD2573165.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            2804..3295
                     /locus_tag="NBL02_002527"
     CDS             2804..3295
                     /locus_tag="NBL02_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HCD2573166.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            3283..3837
                     /gene="srlA"
                     /locus_tag="NBL02_002528"
     CDS             3283..3837
                     /gene="srlA"
                     /locus_tag="NBL02_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HCD2573167.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            3855..>4849
                     /locus_tag="NBL02_002529"
     CDS             3855..>4849
                     /locus_tag="NBL02_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCD2573168.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         1684 a          759 c         1023 g         1383 t
ORIGIN      
        1 aaaatttttt ttgctcttcc attagtacac attttttagc tgaagctgta aatttcatgt
       61 cttaattttt tttagtgagc ttttatactg gatttgtaag aaaaaagaag gagaggatca
      121 catatgtcgg attggttgga tattgcagga aaagtagtaa ttgtaactgg aggttcctca
      181 ggaattggaa gaagtatcgt cgaaaatttg ttgaagcaga atgcacaagt agcgaatttt
      241 gatgtaactg aatgccgtat acagcatgaa aacctactat ctttaaaagt cgatgtgtct
      301 tctaaaacgg acatagaaga agggatatat aaagtaatga aacatttcaa gacgatcgat
      361 ggtctagtca ataatgcagg aataaatatt ccttctcttc taatagatag aaatcatcca
      421 aaaagcaagt atgaattatc agaacaagtt tttgataaga tgatcgctgt caatcaaaaa
      481 agtgtatatt tgatgagcca agcagttgga agaatcttgg tgcaaaaggg atcaggagtg
      541 atcgtgaatc tttcttctga atcaggattg gaaggatcag aaggacagag ctgttatgct
      601 gctacaaaag cagcgatgaa cagttttact cgttcttggg caaaagagtt aggaaagagg
      661 aatgttcgtg tggtaggtgt tgcaccaggg atactggaag aaaccggttt gagaacacaa
      721 gaatatgaag aagctctttc ttatacgaga ggtataagcg tcgaacagct gagaaacggt
      781 tacagtcgaa caagtacgat ccctctagga aggagtggta agctgcaaga agtggcggat
      841 ctagtctgct attatctttc agaaagatca agttatatca ctggagtcac gactaatatt
      901 tcaggaggta aaacaagagg ataaaggata ggggggacgc acgtgacgct agtaaacaga
      961 tggtaccaat tattgaaatt actagtcact cacaagaaag tttcaatcca ggaagtgcaa
     1021 aaagaaatca aaaccagtaa tcaaaccata aaaaaagata ttggggagtt gaatgaagaa
     1081 attgccggta ttgcaaaaat catccaaaaa aacaaacatt ttgaacttga aatttatgat
     1141 tttgattttt ttgatgagat catgcaagga aaattgaaaa cagccagtga tttcaattcg
     1201 gcatccaaaa gaatcgctta tttactaaat aggctgatcg aaacagagga tttcttacgt
     1261 atggatgatt tgtctgaaga agtcggtgtc agcagaggaa cagtggtcaa ggatctaaag
     1321 acattaaaag agatgatcaa tgattttgat gtaaagatta caggcacacc aaataaagga
     1381 ctaagaataa taggagatga gctagaatta cgactacttt tattttattt tgtttctccc
     1441 tacttttcag agacgaaaac agaacagttt ctagaagaaa aacaagcaat caaacatttt
     1501 cagaagaaga cgaatgctag taaacaagaa atcacccttt taacgaaagt tatcggtatt
     1561 tctttgaata gaatctctag taactatctg ttgaagaagc ccatcaatcg ttacagtggg
     1621 tgttttgaat atatccaaga attcaatgaa ctgatcaatc agttggaaga aatctatgaa
     1681 ctaactttga gccaatatga aaaagatttt atcagttttc ctttgaacat catcaacaca
     1741 agtataacga tgataaggaa agctaactct gatttgcgct tatatttcga ccccatgatg
     1801 atgcgtattc gacgattaga tctcattgat ctggatgagt ccttcttata tcaagagatg
     1861 aaacagcacc tctctttatt gataaatcgg gtaatttttc gttatcgatt gactgattta
     1921 ttttatggtg agatagaaaa aaagtatcct ttttcttacc aaatcgctag cgaatgcata
     1981 caagcattag aagctatctt gcaacataag atctctaaag tagagatcag ctacctaacg
     2041 ttgtatttgg agttagcatt tcgatatcaa aaagagcatt tttcctctaa aaaagtagcc
     2101 attgttcata cgacaggaaa aggaacagca ttaatgatcc aaagacaaat caaaaggata
     2161 ttggggaggg atatacaaat aacacatgtt tcagaagaac aatatgaaaa agtagatcta
     2221 aacaaatatt tcgctgtttt tacaacgatt ccgttaaaaa atgtaaatga agagacaccg
     2281 gtgatccatt tgactaatct ttttgatgac tcttggttaa gggcacaatg gcaaaaagca
     2341 aaaaaaaagc gattagccgg gtttcattct actgtttttt tctatcaaat atggaaagaa
     2401 aaaaagacgt atcaagaata tttaaacaaa ttagtcgctc ctttgatcag aggctattat
     2461 gtggatgaag aatttgaaaa aaggatcttg aaaagagaat atcagcaaag cacatttgtt
     2521 aacaaagaat ttgcttttcc tcacacaacc aaccagctat ctcaacacat tgttttatca
     2581 gtagggctaa taactgatca aggtttagca gaaacaggag gaaatggagt tattttttta
     2641 tttgccatac ctgaagaaat gacggctgaa gttgagaatg aattatttga gctatacgat
     2701 atcctatttg aactcataaa aatgggaata gatgagttta ttgaattagc accgacacaa
     2761 gaatcatttt taaaactaat caaaagtaaa ggagagagac ttcatgaatg atatgtatct
     2821 attcatagtt ttcgcagctt cagcttatgt gctgcaagca gttatcagtt ggaagcaact
     2881 aaagcatttc aatcaaacat atagcgaact gcgaagaaaa ggaaaagtag cgattggtaa
     2941 acgacctggg aaaatccgtt caggcacgat tgttatgcta gctatagacg atcatgatca
     3001 agttttagaa gtcagaaaaa tgcaaggggt tacatctttg gcaaaatttc attcattacc
     3061 tgtctatgcg ggtgaagata tccattacat cgatcgttac catccctcag ttcgagcgga
     3121 agatcgtttg acacaagcag ctttagaaaa tgccagagaa gtatacttac gaatagaggc
     3181 aggaaatgaa aaaagtgaac aaccttcatt gccttttatt gctatgctca ataattttcg
     3241 gatatggaaa agtacgatca aagaaaaaag gagtgacaaa cgatggatat cataacaaaa
     3301 tttgctgagg gatttatgag gctgttccaa acaggtgcag aaacattcat aagctggatg
     3361 acaggaatcg taccagttgt cttgatgctg atggttgcaa tgaatacgct gattgctttt
     3421 ttaggtgaag accgagtaac gaaagtagcc aaattatcag cgaaaaatcc gcttactcgt
     3481 taccttgtgt taccgttctt atctgctttt atgttgggaa atcctatgtc atttacaatg
     3541 gctcgtttcc ttccagaata ctacaaacca agctattatg ctgctcaagc tcaattttgt
     3601 catacgagca atggtgtatt cccccatatt aatcctggag aattgtttgt atggatgggg
     3661 attgctcaag ggatcgaaac cttagggcta aattcgatgg agctggctat ccgatacttg
     3721 cttgtgggac tgctgatgaa ctttattggt ggttggataa ctgattttac tactggattt
     3781 gtatgcagac agcaaggaat cgttcttagc aaaaaagtag agctttcagt tgattagaga
     3841 aaggagagtt ttaaatgtat ggtgatatta agatcgtaag aggaaacggc gggtatggag
     3901 gtcctttgat tattaggccg acaaaagata aacacaagtt catctatgtc acaggtggag
     3961 gtgagaggcc aatgatcgta gataaaatag aagaactgac aggtatggag tctatcaatg
     4021 ggttcaaaac atcaatacct gatagtgaaa tcgcgcttgc aatcgttgac tgtggaggga
     4081 ctcttagatg tggaatttat cctaaaaaag ggataccaac catcaatatc gtttccacag
     4141 gaaaaagtgg cccattagca caatatatca ctgaagatat ttatgtttca gcagtagggt
     4201 tagaacaaat acagccacta atggaaactg atgataacaa agaaacagta aatactgcat
     4261 ctccagataa aaagtacaaa tatgatactt ctaaaaagat aaccgagcaa aaagcagagc
     4321 agcaaaactg gatagcaaaa attggaatgg gcgcgggaaa agtagttgct acattcaatc
     4381 aatcagcaag agaagcagta gatacgatga tccggacagt gatccctttt atggctttcg
     4441 tttccctttt gattggtgtg atacaaggtt ctgggatcgg taatgtattt gctaacatta
     4501 tggcaccttt agcaggaaat gcagttggat taacaatcat tggctttatt tgttcactgc
     4561 catttttatc tcctttgctt ggtccagggg cagttattag ccaagtgatt ggaacattga
     4621 tcggtgtaga gattgggaaa ggaaatattc ctccccaatt agcattaccg gcactatttg
     4681 ctatcaatac tcaaaatgca tgtgatttta ttccagtcgg tctaggtttg gaagaagcaa
     4741 aagctgagac agtagaagta ggtgtaccat cagttcttta ttcaagattt cttaacggca
     4801 tcccacgagt tatcattgct tgggtagcaa gttttggatt atatagctg
//
LOCUS       DAHHZJ010000176         4831 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.179, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000176 DAHHZJ010000000
VERSION     DAHHZJ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.179"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..675
                     /locus_tag="NBL02_002530"
     CDS             <1..675
                     /locus_tag="NBL02_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HCD2573169.1"
                     /translation="LSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQGMLEL
                     LGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGIFRMI
                     FAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCWFFGQ
                     VLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKLAKKY
                     YPGKGQ"
     gene            677..1621
                     /locus_tag="NBL02_002531"
     CDS             677..1621
                     /locus_tag="NBL02_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HCD2573170.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            1820..2464
                     /gene="pcp"
                     /locus_tag="NBL02_002532"
     CDS             1820..2464
                     /gene="pcp"
                     /locus_tag="NBL02_002532"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HCD2573171.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            2543..3637
                     /locus_tag="NBL02_002533"
     CDS             2543..3637
                     /locus_tag="NBL02_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573172.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSK"
     gene            3653..3820
                     /locus_tag="NBL02_002534"
     CDS             3653..3820
                     /locus_tag="NBL02_002534"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573173.1"
                     /translation="MKDIQSGNVQETIDKWKGKHYRFTEAQKQALEYYQKLEESLRFN
                     DEARKYLKELL"
     gene            complement(4004..4753)
                     /locus_tag="NBL02_002535"
     CDS             complement(4004..4753)
                     /locus_tag="NBL02_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HCD2573174.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSRGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKLTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT         1548 a          863 c         1042 g         1378 t
ORIGIN      
        1 cttagccttt taggcgtctt gattgttatt ctcgggtttg catttaaatt agactcgata
       61 ttgatcgtaa tggttgcttt agttgttaca gctttgactg gcggtcttgg cattcaagga
      121 atgttagaac ttttaggtac aagttttgta aacaatcgcg ggatggcgat ttttatcatc
      181 attatgttgg caacaggaac gcttgaaaga aatggtctga aagaatcagc ggcttccttg
      241 atcaagcgat tcaagaaagt atctgctggt atgattgtag atatttatgg gatctttcga
      301 atgatctttg cggcttttaa cgtgagtttt ggtggagttg ctggatttgt tcgtccaatc
      361 attttaccga tggcattggg gaccatcgaa tcaaaagggt tagaagtcca tcctaagcat
      421 gaagaagaac taaaaggtat ggcttcagct atggaaaatg tctgctggtt ctttggccaa
      481 gtactattca tcggaggagc aggggcgttg ctagtccaat caacactgaa agatctagga
      541 tatgacgtaa cattgggaca gcttgcttta gtggaagttc cagtggctat cacggcttta
      601 gttgtggcaa gtgtctactt ctatcttaaa gaaaagaaat tagcgaagaa atattatcca
      661 ggaaaggggc aataagatgg acttttttac aaatcctgat attttattag gcgataaatt
      721 actggagatc ctttatatcg tgatgggact cgtattgatt tacactggag taagaaattt
      781 aacagataaa accaatcctc atcgttatgg gtcagccttt ttctggacgg tcttaggggt
      841 agttattgca ggaggaagat ggcttccggc aatcgtgaac ggtggcttga tctttgcgat
      901 gaccatacca gcaattgcta agaaagtcag caaaggagaa agccggcttc catcaaaaga
      961 atatatggaa aaaatgtcag acaaactagg aatgaaaatt tttattccag ctttgagtat
     1021 cggtgtcttt gcgatcttgt ttgctctatt taccaattta ggtgctttag tcggcgtggg
     1081 cgtcggtgtt tttgcagcca tgctaatctt gatgttcttt tctcgagata atcgaccaag
     1141 tacattttta gatgatgcag cagatatgct gggcacagta gggccgttaa gcatgttgcc
     1201 aatgctttta gcttctctag gtgcggtatt tacaagcgct ggagttggga cagtgatttc
     1261 caatgcagta ggtacgatcg ttccagaagg aaatgtgaac atcggcatca tcatttacgc
     1321 tcttggcatg gtgatattta cggtaatcat ggggaatgca tttgcggcca tcaccgtgat
     1381 gacggttggg atcggtgcac cattcgtatt tagtcatgga gcgaatccag cgttaatagg
     1441 aatggttgca ttgacttgcg gtttttgtgg gaccctgctg acaccgatgg ctgctaactt
     1501 caatatcgtg cctgtagcga tgctggagat gaaagacaaa tacggagtga tcaaaaacca
     1561 gttgtttatt gcgttattta tgctagtttt ccaaattgct tatatgattc tatttaaata
     1621 atagaagaga ggggttgtga cagaataatt gtcgcaacct cttctaacat tgtagtggaa
     1681 cactattttt ttataagaat atattaagtt tgatctgtta tgttatttaa actattgttt
     1741 cctttattta cgctgttcaa ggaaaatggt atgataaaag ataaccatat gttaaatcaa
     1801 tcggacggag gaacaacaga tgaaagtact agtaacaggt tttgatccat ttggtgggga
     1861 caaagtgaat ccagcctatg aagcagtaaa aaaaatgcca gatgaaatca gcggtgctga
     1921 aatcatcaaa gtggaagtac cgaccgtatt cgaaaaaagc agtcaagtag tcaaagaagc
     1981 catccaacag caccaaccag atgtcgtgat ctgtgtcgga caagcaggtg ggcggtcagc
     2041 agtctcgttc gaacgtgtcg caatcaatct agcagaagca agaatccctg ataatgaagg
     2101 aaatcagcca ttcgatacgg ctttagaaga aaacggtcca gccgcttact ttacctcatt
     2161 gccaatcaaa gcaatgacaa aaaatgtaca agatcatggt ctaccagcgt acatctctta
     2221 tacagcagga acatttgtct gcaatgatat catgtatcgt ttgcttcatt tgatcgaaac
     2281 acaattcccg gcaattcgag gtgggttcat ccacgtgcca ttctcacccg atcaagtgat
     2341 cgatcgtcca gtgggtacgc cttctatgtc tttagaagac attgcttctt ctttgactta
     2401 tgcagttgaa gcagcagtag aaaataaaga agatatttcc ggaaatgccg gaacaactca
     2461 ctaatttagc aagaaacact ggctggttgc taacgttatg ttagtagcca gctttttgta
     2521 attagaaagg aaagaaacag ccgtggaaga atggcaatcc gtctttgaag aatggtttcc
     2581 aaaagaaatc agcaaaagct atcccatcaa aatttctaaa caatacacaa gcagtcaacg
     2641 ttgggagatc tatgcaaaat taacgaaaaa acagcgggaa ctagtcgata agcaccgccg
     2701 atatctaatc agctctcgtt ttatggaaga acattattta gctgcaacag attgggtgtt
     2761 ttctgacttc aagatcaatc cgttttttcg aacaaaacga agccagcaaa aattgtattg
     2821 tgagtgtggt agagaattaa aagtgcaata catcgttaaa tcgccaaaaa ccggaaagat
     2881 cctaaaacta ggcatcaacc actttgccga tcacctgcat gtctctccga cagtggcagc
     2941 ttcgatccat cagggaatga caaaagttga tctggcctta gacgaattat tatggcttaa
     3001 gcagaagaat atcgattttc ctgaaggact atggcaaaaa tactgttttg tcttgtacca
     3061 aaatcgtcgg atgaaacagc catatttacc ggatataaaa ttagctcagc ggctcgcaga
     3121 atttcgccaa gtagaaatgc cgatttacat agcggattat caagcattgg aaaatgaaat
     3181 caagaaaata agtgaacaca tcaatgggca gtcaaaaaag cgacagataa aaaaagaact
     3241 atttgacgac tttgcagaag aattagtaaa ggatgtagaa gaattcttga tcaactatcg
     3301 cgcattttta cgtaaagatt ggcaatccat cgtatatgaa gaagtgccag tacatcctaa
     3361 tgcttatttt gaaacattta tttccgtgtt gagaaagaca aaacgtcaac gaacaccaga
     3421 agtcactgct cagatggaat attttgcgaa aaaccaacga ttcatccaac caaaaatcta
     3481 tctatttatt tggaagcaat attgtcgcta tggttttaca gaagggttct tcgacagcat
     3541 tcctcggata gtcagaaatg gttttttaaa agtattgcgt aaagaacgag aagctataca
     3601 atctgctgat aagaaagacc gtacagtctc aaaataaaaa tggcaactag tagtgaaaga
     3661 tatccaatct ggaaatgtgc aagagacaat cgacaaatgg aaaggaaaac attatcgttt
     3721 tactgaagct cagaaacaag cgttggaata ttatcagaag ttagaagaat cactgcgttt
     3781 caatgatgag gcaagaaagt atctgaagga attactataa aaaatagagg ctgggacaga
     3841 agcgtttaac tccaagaaat aagaagaaat tcacgaaaat tgcttttcaa atttttgtga
     3901 atttcagctt atttccgaag gagttgcttc tgattccgcc gtttatacgt gtttagagcg
     3961 tgagacaaaa gtgttcttta cttttgtccc acgctcagtt tttttatgcg tgttgttgtg
     4021 cgttgtagta ttcttcttcc attttttgca tacgttcacg ttctttttgg ttataccaag
     4081 gtgaaacggt aaatgcaagg acatcattca gcaagtaaac agagctgttc acgaacatcg
     4141 ccaaacttgc agatccatgc gtaaatgaaa tataccaaag gaccatttga gcaagccctg
     4201 aagctaacca ccagtaatat tggttgttgt aacgcaagaa actgatgata ccagcagtca
     4261 agctaataga aaaagcaatg gcatctaccc acggccgagg atcatctgta taacgaccaa
     4321 tcaggaatcc tgaaacaaga tagacaagga aagtactgat gattgcgata aaccagcttt
     4381 ttccagttaa ttttcggatt tttgaagcca tattatgatt ccattcttta cttaacaata
     4441 cggggatatc caatgtgatc atataagcaa tctgttcacc gatacttaaa tagttctttg
     4501 ctgaaaaccc aacgataata aaacaaatcg cagacaaaat acctaaaata ccgttgactg
     4561 attttgccgc attgatggat aaaatacaca atacaccaag cgtggtaccg ataaatgtaa
     4621 taacggacag ccaagtgatg ggactattga caagagtcat gacctggcag ccacgactga
     4681 aaaagaaaag ataataattt tgttgcggcc agccttttag ctgatgagct aaaaactgaa
     4741 aatatgactt catgtatgag gactccttaa gacactaaat gtagtgtgtt ctgtatttac
     4801 taacacaatc tattgtatgc ttttcttgaa a
//
LOCUS       DAHHZJ010000177         4742 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.180, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000177 DAHHZJ010000000
VERSION     DAHHZJ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.180"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(492..995)
                     /locus_tag="NBL02_002536"
     CDS             complement(492..995)
                     /locus_tag="NBL02_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HCD2573175.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1354..2379
                     /locus_tag="NBL02_002537"
     CDS             1354..2379
                     /locus_tag="NBL02_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCD2573176.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNLMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2702..3580
                     /locus_tag="NBL02_002538"
     CDS             2702..3580
                     /locus_tag="NBL02_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HCD2573177.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            4056..4172
                     /locus_tag="NBL02_002539"
     CDS             4056..4172
                     /locus_tag="NBL02_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HCD2573178.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
BASE COUNT         1580 a          761 c          855 g         1546 t
ORIGIN      
        1 tatacttaaa aaataacgac acagaaaata ccgtgtctca gatttctcta ctacttctaa
       61 aaactactgt ttttttcctt tgaaatgcct tcaaaaagta ggatcaggca gaatatactg
      121 gcagaatcaa ggagaaggga gcctaaataa ggagaaaaac catagaaaat ataaagtaat
      181 tacttataaa acaagctttt tatagaaaaa taatatacct gagtagggta taaattaaga
      241 cggaattcct ttctagattg agatattgac taggaagaat gttaagattt taagccttta
      301 agctaaaaaa aaaccctttt tgttaaataa ggggaatata gcctgtcagt ttcaaactgc
      361 aagtcgtcct gctgcaataa tatagtagat tagagatgaa aatagatttt tttaaaccaa
      421 tgactgaata aaaagaccgt catagataca cattggaatg tgtctattac ggtctttttt
      481 tacgaacgat tttattgaag acggacagtt cgggtcaaaa ataaaaaagc tacgatcaga
      541 aataagaata aaatgtatcc caaaataatg gctagacggt ctaacttatt tgtcgataga
      601 tgtttgttta gaagttgata ggcccataaa cctatcagtc ctcctgttgt attagttatc
      661 aaatcagtga tgtccgctac gccaataccg aataggaatt gaatcaattc tgccaagaag
      721 cttacaagga aaataatccc taatttttta gaaaaagaaa gtctcttgaa attgacattc
      781 aaaagcaccc caaaaggaaa aaagacaatc aagttataag tggtctcacg taataccgtc
      841 gtattttcaa aagaaaaagt agaaaaaggg acgagattga cattgctgta agaataagtg
      901 agaaattgtg gaagattaaa tgatagcttg aataaaatca accataatag aaagaataaa
      961 taaatcccta ataaaaattt tgatacatac cgcatagaca tctcctccat ttaaaaagta
     1021 taccatcgaa aaccgctgaa aaatcataaa aaagtatgaa gtttaaggac caacgtagtt
     1081 atcgtacgaa aattagaagc ttttttttgt tgggaatccg gtttctttaa caaagctaag
     1141 atagcgagtt gcttggaaaa gttgctatct tttttctgtc aaaacgactc ggaatgttcg
     1201 ttttttgttc taatttctcc aagttgttta ctttttgata ctaagtttat gaaaaatcaa
     1261 gaaatagttg ttatactagg taatgctgac aaacgggatt ggcatttcat cttggttcag
     1321 ctttttttcc aattactagg aaggagggat agattgttca agacttattt tcgtttcaat
     1381 aaaatacaag ataatccttt tcgtatcatg aatctgatgc tacttatctt tattgtatta
     1441 tggttaggtt acataaatca tgattttttg atttcaagtt tttcatcatt agggatcttt
     1501 acttttttct attatcaaaa cataccgatg aaatatttgc tgaaacgtat gtttctgatc
     1561 ggttgcggac tgttggttgc tttcgttttg gggatcttat cgacttatgt tttatggctt
     1621 gaaccatttg ccgtagcttt ggttgctttt tccagcagat tcgtgttgcg attattccat
     1681 atttctaaac caggtggctt gttttttgcg atgctttctg ccatgggaac gagcatgcag
     1741 ctgccaatcg ctcaattacc gatcgtctca ctttattttt ttatgggtgt cgtttttgcc
     1801 ctgattgcgg cagtgattac aaaattactg gattcacggc ctgagcagac aattgaaaaa
     1861 gcaactttaa aagaaagatt tcacgaagag cctttagtga tcattgattc tgttttctac
     1921 agcgctgcct tatttctttc cgtttatgtc agtcacggct tgaatctgca taatccttat
     1981 tggttgacct tgtcatgtgc atcgattttg ctagcagaaa acttggatgc gatgaagcat
     2041 cgacaagtcc aatatctgat tggatcgatg ggtgggttat gtgtgtcggc attcttgtca
     2101 tttgtgccgt ttacgcagtt gcagaccatt tttctgatca cttttctata tggaatcgca
     2161 caatttctag ttgctagaaa ttatgcagtt gctaatattt ttcttaatct gatggcgttg
     2221 atgctatcta cacttattcg tggagcatac ttgatttctt tgatcgaata tcgtttttta
     2281 gggatcgtca taggaagttt catcggttta ggtgttgcgt gggtaatgac ggttggtttg
     2341 caacattatt taacggttgt tcgtcaaaaa ttagaataga aggacaaata tatgtgccaa
     2401 aagaggttat gacaagattt ttgtcacaac ctctaattca aggtgttcaa aagccagatt
     2461 ctcagcatta agtcaaattc acacaaaaat gtggaaagga actttcaaaa aaattttata
     2521 tttattctat caaaaaattt tgtttatgat ggtaactgaa taaactttcc tgagatggtc
     2581 aatgctttcc ttgaaagtct tacagatatt ctttcttcat agaaagttta agttttatag
     2641 ttagtgtaca ataaaagtat gtttatacca tatggtgtcc ggataatcag tcatttaaac
     2701 aatgaggttc aaagatatga aaaggggaaa tttgatgaag aaaataagca atgaaatcca
     2761 aattctggca gaaactttaa ctcttcccaa agtcgttata gagcaaatga ttgtacaagc
     2821 aaaaaacatc cttgatgagg tgtttgagca atatagtcat gaactaatgg atcctaataa
     2881 agcaaaggcg actgcaaaga aaatagacaa agtctatgaa gaggaacctt ttgcagcatt
     2941 agctatttat ctatatgcag cttctcaaag ttggaaaaaa atttatcaac cattgaagat
     3001 tccaagaact atctatttgg caacaatgaa tgcttttact agatttattc atgaacattt
     3061 ccaagtaaca ggaagctatc gctttgaccg tggtttttgg atttggcatt acactagtgg
     3121 cttgatcttt cggattggtg aattggagta cgaaatgaca tattttccga aagggtacac
     3181 ggatctaaga ttagaaggaa agcagtgcct ttctcttcat attccttccg atgcagattt
     3241 atctcagaat aagataagag atagttacga aagtgccatt cgtttttttg aactttattt
     3301 tcctgattac cattaccaag tgatgtacac agatacttgg ttactatcac ccaatcttac
     3361 taaatggttg aaaaaagagt caaaaatctg tctttttgca gccgattatc gattattatc
     3421 agttgatgaa caggacgata gtggagtccc ttggatcttt ggacgagtag atgcccaaat
     3481 acatgattat ccagaaagta cgagtttaca gcgacaagca aaagagcaat tgcttgcagg
     3541 cgagcacatt ggctctggct taggtattct tacttcataa gtttagtact tcgaaatagt
     3601 ttaaaagagc gtgagacaaa agtaaagaac acttttgtct cacgctctaa acacgtataa
     3661 acggcggaag cagaagcaac tccttcggaa ataagctgaa attcacaaaa atttgaaaag
     3721 caattttcgt gaatttcttc ttatttcttg gagttaaacg cttctgtccc agcctctttt
     3781 ttagtgttct atcgaacaca cagtacgttc aataggtaaa ctagctataa caatagatat
     3841 taaagttaac ggacaagagg gcctttttta ggtagctaaa acgtaataat gaaaatttat
     3901 ttccagagat aggagtcaat cagctagtct gatcgactgt tttaacaagt tgactttcat
     3961 tttaactttt gataccattt aaaacacaaa tcgtaattgt ttcgatttgt gttttttagc
     4021 atgactacaa tcaaagaaga aagtgggtcg tccatatgga aaaaacagat atctcaagtg
     4081 cataccgccg cttgaaaagc ccaaatatca aaacacgaaa acgtgcgctg aagatcatca
     4141 aagatgtgaa acggaatagt gggaaaaggt aagaagaagg aaacttaatt aaaaaaatag
     4201 ccgaaaaacg tttttcggct attttcaata atcggttttt gtgtaaggtt tccgaaagaa
     4261 ctgctgttga ttttccattt ttaacatatg ataaattggt agaatctttc ttttcaaatt
     4321 gataccattc cctaattcca gttatatagt taactacaga accacaaagc acgtacgttt
     4381 ataaaagtat tatctgtagt tatttttcaa ttccctttca acctcttatt ttgaccagta
     4441 gtattgatca ctttaccttt taacaccttt gttcgttttt gaaacatttt tagtcaagaa
     4501 tggaaacgta actacataat caaaggtttt gcgaaatatt tcattaaata tttcatgtag
     4561 aagcgagcaa tacggttgca aataaccatt atcgtttaat aagcagttac gaaagctccc
     4621 aatatctcta acgttcagct cactccattt cttttcttca aaaaaatcag tgattcgtat
     4681 ctctttctat ttgtcgaaaa agttaacttt aaaaagtaac gccgaaaaaa tatgcaaaaa
     4741 aa
//
LOCUS       DAHHZJ010000178         4723 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.181, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000178 DAHHZJ010000000
VERSION     DAHHZJ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.181"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(43..816)
                     /locus_tag="NBL02_002540"
     CDS             complement(43..816)
                     /locus_tag="NBL02_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCD2573179.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            960..1310
                     /locus_tag="NBL02_002541"
     CDS             960..1310
                     /locus_tag="NBL02_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HCD2573180.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1291..2007
                     /locus_tag="NBL02_002542"
     CDS             1291..2007
                     /locus_tag="NBL02_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD2573181.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2007..3455
                     /locus_tag="NBL02_002543"
     CDS             2007..3455
                     /locus_tag="NBL02_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCD2573182.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(3525..4034)
                     /locus_tag="NBL02_002544"
     CDS             complement(3525..4034)
                     /locus_tag="NBL02_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573183.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4024..>4723)
                     /locus_tag="NBL02_002545"
     CDS             complement(4024..>4723)
                     /locus_tag="NBL02_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573184.1"
                     /translation="RLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSALANQVQDRL
                     SFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNKQELINWHLT
                     GLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVLKTTELPDIR
                     DILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLILSLFPFTFF
                     IHHLQSRRPGRNET"
BASE COUNT         1611 a          812 c          956 g         1344 t
ORIGIN      
        1 aagcgatttt cttaagaaaa gaggtagctt caaaaaaaga tcttaaatca gatcaaaata
       61 cttcaatgcg tggacaattc cgccttctgt atttttcttt gtgataaagg tagaaagttc
      121 ttttaatccg tcataggcgt tgcccatagc gattttgttg tcgcaagctt ccaacaaggc
      181 aaaatcattc ggaccatcgc caaatccaaa agtaggaact ttttccagct gaagttcttt
      241 ttgcagacgt ttgacacctg ccccttttga gattcctttc ttcactgtat cgatcgagta
      301 aggaccattc cgataaaaag ttagctcagg aaaatgttcg tgatagtatt cgtcacctga
      361 ttgagataag atcagcaaca tatttactgt gttttcttta aagcctaaag gatcaatttc
      421 cggaacatct gaatgaatga agtcgtaagc attgatcatt gtttggctat gaccggtaca
      481 ccagattttc tgatcgttat agtaagaaag ttcatgaccg ttcaactgta catgatcata
      541 cattttttga cattcttctg aagaaaatgt ttgagaatag atttcttcgc cgtctactcg
      601 aatgaaagaa ccattcatgg tgaccgcaga agtaatgccc gcatcttcca tgatagagtg
      661 gatttctgct tctgtacgtc ctgtagcgat gacggggaga acattgtttt cttttaatgc
      721 gttcatagct tttgccactt cgggtgtgat ttttgatttc gcatctagta atgttccatc
      781 aagatcaaag aaagtgattg cacgataatt tgacacaaat aaaactcctt ttttattgga
      841 ataagtttat cttaacaaag aatgaacaaa agacaaacct ttcgtgaaga attcctgaac
      901 atattggttc agtcaacaat ttagagtata ctataagaag agaatttagg agggaaagaa
      961 tgattttttt cagtattggt atcttattct taataattgg cagtcttttt cggattctgc
     1021 cctcaaaagg aaatctgcct ttttatgggt atcattcgcc tcttgccgca aaaacagacg
     1081 ctcattggcg gctggctcaa aagacaagcg gaaactggtt ttttcttatg ggcttactga
     1141 tggcattgat cggctattat ttaaagacat ctgggcatac gaattatttt ctgattgaaa
     1201 tgcttctgct agtatttcca attatgccta tttttattat gacagaaaag aaattacaga
     1261 aatatgacct ggaaactgga gggaacgata atgaatattt taatgattga agataatcaa
     1321 tctgtttctg aaatgatgca gatgtttttc ttaaatgaag gttgggaagc aactttcaag
     1381 tatgatggaa aagaagggct cgatgccttt ttagaaaacc cacaacattg ggatatgatt
     1441 acactagacc tgaatttgcc cacactggac ggtatgctag taggaagaga gatccgcaaa
     1501 gtttcaagta ctgtgccgat tatcatgctg acagctagag attctgaaag cgatcaggtg
     1561 atcggcttag agatgggggc agatgattat gtcacaaaac ctttcagtcc tctgacgctt
     1621 attgctcgaa tgaaagcttt gcatcgccgt agtgagttgg cagaagcaag agaagaagca
     1681 gtagtttcag atgatcattt tgatatcgag actagtcatt tcaagatgaa tacgaaaaca
     1741 cgagaagcgt atctggacaa ccggccaatt gaaggactga cgcctaaaga gttcgatcta
     1801 ttgtttactt tagcaaaaaa accacgtcaa gtattctctc gggagcagtt gcttgaatta
     1861 gtctgggatt atcagtactt tggtgatgaa cggacagtgg acgcccatat caagaaatta
     1921 cgacaaaaaa tcgaaaaggt cggaccacaa gtcatccaaa ctgtttgggg agtcggctac
     1981 aaattcgatg attctggtgt tgcctaatgc gttatttgta tcagcaactc ttagctttct
     2041 gcggaatggt cgcgttgatc ttattgatcg ttggagtttc ttttacacaa ctgacaaaac
     2101 aaacgattga agaaaacaat taccagcaac tgtttggata tgctgaatca gttgaaaaga
     2161 caacgcaaac ctatgccgat acgctcccgc agtttagtca agatcaggcg tttcaaaatg
     2221 cattgctgct gacagagcaa gtactgacag aacaaaatgt gaactttata tttatcgata
     2281 aaaacgaacg tgtcatttat ccgactaata cagctttact gaattttacc ttaacagctg
     2341 atcaatggaa aaatctgagg aatggacgtc aagaaaaatt cacatcgaat aaaaatattt
     2401 taggacaagc acaggcgacc tcttacgcac ttgtgccatt taatttgaat catgaattct
     2461 atggagcact tgtagttagt caacctgcgc gaaatatcga taacagcgtt cgtccaatta
     2521 cactgaacct gttcaaagga tttatttttt ccagtatcgt tgcagttatt gccagctatg
     2581 cttttgcagc attccaagtt aagcgtatca atcggttaag gaatgcgacg aaagaagtga
     2641 cgaatggtaa ttttgatgta cagctccccg tacatgacaa agatgaattt gatgagttag
     2701 cagatgattt caataaaatg acgaattcat tgaaagaatc tcaagcagaa attgaagaac
     2761 aagaaaatcg tcgaagacaa tttatggcag atgcttctca cgagatgaga acacctctta
     2821 cgacaatcaa cggacttctg gaagggttag aatacaatgc aatccctgaa aatcagcgag
     2881 aaaacgcgat taaattgatg aaaaatgaaa cagagcgtct gatccgtttg gtgaatgaaa
     2941 acttggatta tgaaaaaatc cgtacgaatc aaatcagtat ggtcattaaa aaattcaatg
     3001 gaacagaaac attgaaaaat attgttgcac agttagaagc taaagcagaa gcagcaggag
     3061 atacattgac attgaaagcc gttgatgata ttgatgttta cgctgattat gaccgatttg
     3121 tccaaatcat ggtcaatatt attcaaaacg cgatacagtt tactgaaaac ggccaaatca
     3181 cgattacttt agaaaaaggc tatcttgaaa cgatcatcac gattgaagat acgggaattg
     3241 gaatgtctga acaacaaatg aaaagtatct gggatcgtta ttataaagta gatccttcaa
     3301 gaaaaaatac aaaatatggt gaatcaggat taggtttacc aatcgtgcag caacttgtac
     3361 gcttgcataa aggaaaatta gaagtagaaa gcgaactagg aaaaggaaca aaattcgctg
     3421 ttcgtttgcc tgacgtagaa atcaaaaaag agtaaaaaac aagcggtata aaagatcatg
     3481 aatcgatctt ctataccgct tgtttttact ataaaatgat tgatttagcc cgcgggctct
     3541 aaacgatcaa tataaggact ttgagcaata gtagcatcat gagtaaagag taagatacct
     3601 tgattttctt catgagctaa ataagaaagc aagtggagta gctcttggat ctccaataca
     3661 ctaagttcat caaatgtatc ttcaataaca atattgtttc gcttggaaag caaggcatga
     3721 agtaattgca gctttacttt ttcaaaagac gtcaactcag ctgccgcaca attaagcaag
     3781 gaaggagaga tatcaagttt ttcttgatag gccaaacgat cttgtttgat ttctttttcc
     3841 ggtacatcca acaggacatg gtctttaaga ttcagataag gaaacatcgg ccaatttttt
     3901 tggatgatcg caaaattatc cagttttgca acaagaacct cttttttcaa gtgctccaat
     3961 attttatgct gtttttcgag aggacacaaa ataacggtca agtgttcagc atgaatcaaa
     4021 tattcaggtt tcattacgtc caggcctcct actttgcaaa tgatggataa aaaaagtgaa
     4081 agggaataga ctaagaatga gacagcatcc actgagccca ataagagttt gggcaaatcc
     4141 cttaagtata tctgtgaaaa aaagatcatt ttggaaatcg atggtaaaaa aagtctgttg
     4201 atccatcgga attgtaagtc cgcttaccga agtcaagata tcacggatat ctggcagttc
     4261 agtagtcttt aagacaagaa aattgatcct ttgaagaagc gcttcataaa ctgactggaa
     4321 gcttacgatc cacaataaaa ccaagataca acaaagaaag agaggaaaga acagctgcca
     4381 gaaaataaaa aggaataatt tggttttaga aagaccagtc aggtgccaat tgatcaattc
     4441 ctgcttgttt tgcctgactg aatagtaaaa gaaaaagata aaaaggaagg caaatagaaa
     4501 aaataaactg aatatgcctt ttatataaaa tcggctagct aattctaaag ccggaagaag
     4561 ctctgtagat gcagaagcgc caataaatga aagccgatct tggacttgat tggctaaagc
     4621 cgattgcaaa tggtaaagag tcaggcatgc tatcaataaa aaagcagaaa agcttgaaaa
     4681 taaacaatag agtattgtct gctttttgta ataaattaat cga
//
LOCUS       DAHHZJ010000179         4711 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.182, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000179 DAHHZJ010000000
VERSION     DAHHZJ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.182"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            107..358
                     /locus_tag="NBL02_002546"
                     /pseudo
     CDS             107..358
                     /locus_tag="NBL02_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            451..1245
                     /locus_tag="NBL02_002547"
                     /note="aph(3')-IIIa"
     CDS             451..1245
                     /locus_tag="NBL02_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HCD2573185.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1655..1738
                     /locus_tag="NBL02_002548"
                     /pseudo
     CDS             1655..1738
                     /locus_tag="NBL02_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1787..1870
                     /locus_tag="NBL02_002549"
     CDS             1787..1870
                     /locus_tag="NBL02_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HCD2573186.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1995..2732
                     /gene="erm(B)"
                     /locus_tag="NBL02_002550"
     CDS             1995..2732
                     /gene="erm(B)"
                     /locus_tag="NBL02_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HCD2573187.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2995..3858)
                     /locus_tag="NBL02_002551"
     CDS             complement(2995..3858)
                     /locus_tag="NBL02_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HCD2573188.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3860..4132)
                     /locus_tag="NBL02_002552"
     CDS             complement(3860..4132)
                     /locus_tag="NBL02_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCD2573189.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4149..4358)
                     /locus_tag="NBL02_002553"
     CDS             complement(4149..4358)
                     /locus_tag="NBL02_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HCD2573190.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4456..>4711)
                     /locus_tag="NBL02_002554"
     CDS             complement(4456..>4711)
                     /locus_tag="NBL02_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HCD2573191.1"
                     /translation="IGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVS
                     TWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1445 a          796 c          865 g         1605 t
ORIGIN      
        1 tttcttggga gttttgagac tactatatgc gtttttttgt tgatttacca acatgtttag
       61 acattttcct ctaggaaaaa attaactagc accacgggtt atcttttata tagaagatat
      121 cgccgtatgt aaggatttca gggggcaagg cataggcagc gcgcttatca atatatctat
      181 agaatgggca aagcataaaa acttgcatgg actaatgctt gaaacccagg acaataacct
      241 tatagcttgt aaattctatc ataattgtgg tttcaaaatc ggctccgtcg atactatgtt
      301 atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa
      361 tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa
      421 atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg
      481 aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag
      541 gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat
      601 aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag
      661 ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg
      721 agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag
      781 attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt
      841 ccctatacga atagcttaga cagccgctta gccgaattgg attacttact gaataacgat
      901 ctggccgatg tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag
      961 ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac
     1021 ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg
     1081 agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag
     1141 gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat
     1201 tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct agatttagat
     1261 gtctaaaaag ctttaactac aagcttttta gacatctaat cttttctgaa gtacatccgc
     1321 aactgtccat actctgatgt tttatatctt ttctaaaagt tcgctagata ggggtcccga
     1381 gcgcctacga ggaatttgta tcgataagaa atagatttaa aaatttcgct gttattttgt
     1441 acatttaact tgacggtgac atctctctat tgtgagttat tagtggtaca gttttcaacc
     1501 gttttaatta taaaaaagtg gtgcattttt aaattggcac aaacaggtaa cggttattgc
     1561 aggtgtattt cttatctatg ggtttaacat ggattttatc attaaaatca tgagtattgt
     1621 ccgagagtga ttggtcttgc gtatggttaa ccctaaagtt atggaaataa gacttagaag
     1681 caaacttaag agtgtgttga tagtgcatta tcttaaaatt ttgtataata ggaattgaag
     1741 ttaaattaga tgctaaaaat ttgtaattaa gaaggaggga ttcgtcatgt tggtattcca
     1801 aatgcgttat gtagataaaa catctactgt tttgaaacag actaaaaaca gtgattacgc
     1861 agataaataa atacgttaga ttaattccta ccagtgacta atcttatgac tttttaaaca
     1921 gataactaaa attacaaaca aatcgtttaa cttctgtatt tgtttataga tgtaatcact
     1981 tcaggagtga taacatgaac aaaaatataa aatattctca aaacttttta acgagtgaaa
     2041 aagtactcaa ccaaataata aaacaattga atttaaaaga aaccgatacc gtttacgaaa
     2101 ttggaacagg taaagggcat ttaacgacga aactggctaa aataagtaaa caggtaacgt
     2161 ctattgaatt agacagtcat ctattcaact tatcgtcaga aaaattaaaa ctgaatactc
     2221 gtgtcacttt aattcaccaa gatattctac agtttcaatt ccctaacaaa cagaggtata
     2281 aaattgttgg gaatattcct taccatttaa gcacacaaat tattaaaaaa gtggtttttg
     2341 aaagccgtgc gtctgacatc tatctgattg ttgaagaagg attctacaag cgtaccttgg
     2401 atattcaccg aacactaggg ttgctcttgc acactcaagt ctcgattcag caattgctta
     2461 agctgccagc ggaatgcttt catcctaaac caaaagtaaa cagtgtctta ataaaactta
     2521 cccgccatac cacagatgtt ccagataaat attggaagct atatacgtac tttgtttcaa
     2581 aatgggtcaa tcgagaatat cgtcaactgt ttactaaaaa tcagtttcat caagcaatga
     2641 aacacgccaa agtaaacaat ttaagtaccg ttacttatga gcaagtattg tctattttta
     2701 atagttatct attatttaac gggaggaaat aattctatga gtcgcttttg taaatttgga
     2761 aagttacacg ttactaaagg gaatgtagat aaattattag gtatagggca cctctaataa
     2821 ctaccaatta attcacctca aaatgaaatt agaaaaaaac agcgaaattt ttaaatctat
     2881 ttcttatcga tacaaattcc tcgtaggcgc tcgggacccc aacatgagga aatgttttta
     2941 taaaaaaaga gtagttaccg ttttttggta actactcttt tcctttttta aggtttaaat
     3001 acctggaagt ttaggtgttt tgggtattgg tggtgtaggt ggctgcaagc tttccaattt
     3061 ttgttgaatt gctttaaatt caggtgtctc ttggtgttta tttagaacca ttttttgctc
     3121 tattcgttct aaagtcggtt gaatttcttt ccctgatact ttacgattca attctttttc
     3181 taaggtttct tttggactaa tagaaggtgt ttccaaactt gaatagagtt ttactccttc
     3241 tctgttatat agccttatat cgctaaataa gcccgtttta tgaagggttt ctaaattggt
     3301 cggtaagttt ttgacaacaa tatcatgcgc ttgttttggt gttgccctgg ctgtcattgg
     3361 atcatctgca tacatggttt catatcgttc aattgttcct aaatatgagt tgattttagg
     3421 tactgccatg acatacattt ttgtttcata acctttggct tgaagcattg ttgcggtttg
     3481 aataggaacg tctgttgttc gtcctgtacc ttcgatcacc aaattatacc cttgatcgct
     3541 caaacggctt atgatcgctt ctgtcatgcg attagaataa ggggtaacgt gttttactac
     3601 gtctttttca taaagtttca ctagttcatc aaaattaggg tgctgttgtt taaaggtatc
     3661 attatcaatg acaataacat tcccttgtgt ttcttcaaaa attgctgatc gcaaactggt
     3721 tttccctgac cctggttgcc caccaagtaa aaaagcggtt ggcgattcaa ccgctttttt
     3781 tccttgaatc aattcttcta aattatcatt taagcgattc tcaaattgtt tgtcagtaaa
     3841 attgactata tttgccatat taagccactt tctctttatt caaaacttgt tttgagtaac
     3901 gattcattga ccgccaatac tcatgaacgg cttgtaattc ttctaaagaa taatcaaaaa
     3961 gatttacacg ttttgcaagc tttaattggt ttaataaatt gacttccaat aattgagagt
     4021 catttttatt taattcatgg ttcaaaacat agtttaatac tcgattataa acgcttgccg
     4081 ataattcgtt aatgatctct atttcaaatg ttttttcata cgtaactgcc atttcatttt
     4141 cactcctttt aaagtttgtc aggtaaatat ttccgtatat attcttctaa ggcttgatcc
     4201 ataacttctt ttacgtttcc gccattcttt gctgtttcga tttttatgat atggtgcaag
     4261 tcagcacgaa cacgaaccgt cttatctccc attatatctt tttttgcact gattggtgta
     4321 tcatttcgtt ttgctttttg tgcgcctaaa tttcccacaa tcactcactt ctttctattt
     4381 cttcttactc ttattttatc atcaacaatc acaaatcaca agtgatttgt gattaatcac
     4441 ttgtgatttg tgattctatt ctttttcgtt ttctaattga ataattcgct caagcatttc
     4501 aaaaaatacg ttcttataca tggataaaac ttttttgtca tagcctttat gctctgtaat
     4561 gccgttttta gaccaggtac ttactttatt acttcgcttg ataatatttt ggaaaaccaa
     4621 gttatcttct ttatgttgct tatacagttc ttccaggttt gattttatcg ttgcgctgtc
     4681 cgtatcaact aaataaggaa caaaaccgat c
//
LOCUS       DAHHZJ010000180         4707 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.183, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000180 DAHHZJ010000000
VERSION     DAHHZJ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.183"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(<1..1147)
                     /gene="nagA"
                     /locus_tag="NBL02_002555"
     CDS             complement(<1..1147)
                     /gene="nagA"
                     /locus_tag="NBL02_002555"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCD2573192.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1223..2059)
                     /locus_tag="NBL02_002556"
     CDS             complement(1223..2059)
                     /locus_tag="NBL02_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD2573193.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2243..3460)
                     /locus_tag="NBL02_002557"
     CDS             complement(2243..3460)
                     /locus_tag="NBL02_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCD2573194.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3609..3788)
                     /locus_tag="NBL02_002558"
     CDS             complement(3609..3788)
                     /locus_tag="NBL02_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573195.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            3843..3926
                     /locus_tag="NBL02_002559"
     tRNA            3843..3926
                     /locus_tag="NBL02_002559"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3877..3879,aa:Leu,seq:gag)
     gene            complement(4166..>4707)
                     /locus_tag="NBL02_002560"
     CDS             complement(4166..>4707)
                     /locus_tag="NBL02_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573196.1"
                     /translation="LLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKR
                     AMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFL
                     VILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFM
                     QFGLTVLGICTFIITVINR"
BASE COUNT         1410 a          992 c          766 g         1539 t
ORIGIN      
        1 atgcgtggaa acgttccacg ccatctagat aagttgcttc caatgtcata tcagggttca
       61 atacgataaa gtctgcatcg cgtccttgag cgatttttcc acatgtatca tcgattttac
      121 agctgattgc tggaatcaga ctagccatca tgactgcttg ttctggtgtt gctaatcccc
      181 agtctacaac atttttaatt gcttctttca atttcaatat acttccagct aagttgccag
      241 attctagacg tgctgttcct tcttttacta ctactggaaa ttcgcctaaa ttgtaatttc
      301 catcaggcat cccaccggcc atcatacagt ctgtaattaa tgcgacatga tcatgtcctg
      361 ctttttccat taggattccc gcagcatttg gatgtacatg atggccatca caaatcaatt
      421 cagaaaagac atgttctaaa gataataacg cacccaccat acctggttcg cgatgattca
      481 atccacgcat accattgaaa gcatggacaa atacacttgc gcctgcttca acagcttttt
      541 gcgcttcttc tagtgtagca ttactgtgtc ctagagaaac aactactcct tcttcagtca
      601 cttgtttgac aaactcttct acaccttgac gttccggtgc taaagcaatt tttttgatta
      661 aaccacctga agcttcttgc cattcatgga aagtatccaa atcaggatcc ccgaaataac
      721 ttgggttttg tgcccctttg tgttcttctg tgaagaaagg accttcaaaa tagattcctt
      781 tgattttagc tcctggtgct tcctgataca ttttgccgat tgtctcagct acatctttta
      841 aacgttcttt gcttgacgtc aatgttgttg gtaaaaatga agtcactcca caagataaca
      901 gaccttcaga catcactttg atgccttctg catcattatc cattacatca tgattcatat
      961 agccgtgaat atgtgtatct accaaaccag gagcaatcca tttgccgctt tggtcaatga
     1021 tgttgacttc tccctcaggt agttctttgg tgtattcacc aaaaagtcca tcggtaattt
     1081 caagatagcc tgcatgtttg acacctgacg ctaaaaagaa tttgtcagca tagataaaag
     1141 ttctcattct attctctccc tttgtttttc tacctctaaa gtactcttat ttagagaaga
     1201 agtcaagaat ggtctacacc atttacaaat tttttgaaat ttgtttcaca gcttgtgcta
     1261 agccttcata gaatgctgct ctttctggtt cttctttacg cttgtatatt aagcttttaa
     1321 gcacaaataa atcattgagg taatagtagc tattcttctc ttgagcccaa tagattcctt
     1381 gattcacctg ttctaacgct tcgtcgattt cattttgatg gaataagaaa tttccataat
     1441 tataaaaaat ttgcgaaaat tcactttttg atcgttcatc agctgattga tgcagcagtt
     1501 ggatgcttcg actcaaatag tagcgtgctt cagccgtttt tcctgccaca ccatatattt
     1561 ttccgataca actaatcagt tgtatttctg tgtcagaaac atggattctt tctttttggc
     1621 tagagtagct taatgcttct ttcaaataat aaagggactg agagatattt tgacgcaacg
     1681 tgaattcgca aacaccgaga taataataat agcgttgaaa gtcagttgct tgatacagat
     1741 tttcagaaat atcagaagat tttagtactt gttctaactt ccgataatca cgtttttgga
     1801 aacaactatc caacagttca aataatttat cgtttgtccg agtagttttt cgagagagat
     1861 tcatcacatg atcaatcgtt acccctagcc gttgacaaat ctgcatcatc accaatacat
     1921 tcggcagttc ctcattattt tcgatccgac ttaatacact ttgtgaacaa atatcatgcg
     1981 aaagcatttt ttgcgtcatt ttttgttctt ttcgaatctc tttaatgatc gttccaaagg
     2041 actcgattaa cgtttccata agtatcctcc ccaaaatatg cgtattcgca ttctattcgt
     2101 attttacttt aggaagtcgc aaacttcaag aaaagacttg caactaaata atatacttta
     2161 gttatttaag aaaactccac tagaaagaga gcctcagtgt gtttgtctgt ggccctccta
     2221 ctgaaaaatt tggtttttaa aatcattttt ggcgataggt tgctaggaat tctctcattt
     2281 tcttagctgt ttgtttcaaa tcatccattt taggaagata aacgattcga aaatgatctg
     2341 gctggttcca attaaatcca ccaccatgta caagtagaat atgatgttca tgtaaaaagt
     2401 ccaaaacgaa tttttcatca tccaaaatat tgaatcgctt tgtatcaatt tttgggaata
     2461 tataaaaagc agcttttggt tttacggctg aaagaccagg aatatcattg attgcattat
     2521 agatatattc tctttgttca tagatccttc cgcctggcag gagcagctca tcgacacttt
     2581 gataaccgcc tagcgcggtt tggataattt gctgagacaa tacattggaa cacaaacgca
     2641 tggaggacaa catattcagc ccttctatat agcctttgac gtgtgactta tctccactta
     2701 ggaccatcca gcctactcga aatcctgcta cacgatgaga tttggataga ccgttcagcg
     2761 taacaacgaa caaatcaggc gctaatgtag caatcggaat atgttctaga ccatccatca
     2821 ctaagcgatc atagatttcg tcggaataaa taatcaggtt attttgtcga gctacttcaa
     2881 cgatttcttc taataattct tttggataaa gtgccccagt aggattatta ggattaatga
     2941 taacaatagc ttttgtccga ctcgttattt ttgatttgat atcatcaata tcaggatacc
     3001 attctgcctc ttcatcgcaa atataatgaa caggtgtgcc tccagctaat gcaatagaag
     3061 ccgtccacaa cggatagtcc ggcattggaa ccagtacttc atctccattg tccagcaacc
     3121 cctgcataca catcgtgatc aattcgctca ctccgttgcc ggtgtagata tcattgatcg
     3181 tcacattcgg aaaatgtttc agttggcaat attgctcaat tgcttttcta gcagaaaaga
     3241 tccccttgga atctgagtag ccttctgagt cacgggcatt catgatcaaa tcacgaatga
     3301 cttcgtttgg tgcttcaaat ccaaaaggtg cgggatttcc cgtgttcaat tttaaaatac
     3361 taatgccctc ttcttgcatc cgctccgctt cttctagaac gggaccgcga acatcgtaac
     3421 tgacaccttc taatttgttg gatttttcaa attctctcat cacttagcca atccctcatt
     3481 tcaaaaagtt agaactttcc aaattttttg ttatttacta tttcaatatc atactccatc
     3541 ctagaaaatt ttgccactta tttaggaaaa acgtaagtat taaaaaaaca caaacaagcg
     3601 ttgagaactt aattgtcatg atagtcattc catagattcg ctgcccaatc cattgcaatt
     3661 ccacaaatga ttcctagcat aaaaccaagc aaaaaacgtc cgaatgcaag tccaatcaat
     3721 acacctaaga tcattcccag tcctgaaaat tctccgtcat atttctccat cttaataagt
     3781 cctcccattt ttgagcttga caactatgta aaacctcgct atcattagtg tgttattatt
     3841 acgcagatgt gttggaattg gcagacaagc atgattgagg gtcatgtgag cgttagctcg
     3901 tgtgggttcg agtcccatca tctgcactac aactcgtgaa gttaattttg cttcacgagt
     3961 ttttttgttt gaacgtttct ttatgccatt atatacatat cactgtatcc tttacaaaaa
     4021 gaacgtacaa tcatgtaaac agcctgaggt gagtatatga aggtcggaaa caagctactc
     4081 gtagaaactt gcaagtgtta aaacaattaa tcgaaaaacg agtaaaatcc ctcacctaat
     4141 actaaagcga gggatttttt gtatattatc tgtttatcac cgttattata aaggtacaaa
     4201 taccaagcac ggttaatcca aattgcataa attcattttt ttgtacactc atgcttttca
     4261 ttagctcttt aactgccatt agtactaaga tggcgataac aacataaagt atatagtacg
     4321 tccgcattcc ggaagcccat actgtgggag aaaatcccat aattaacctt gaacaaaatc
     4381 ccattatcat caaaataact aaaaaaatag aagttttctt attgtcaaaa cttaaataaa
     4441 ttccaacaat taaacatagt aataatgcca agactactaa agtagctatc caagtacctg
     4501 gataaaaaag tgacagtttc gttcctaact ctgtaaagag attattcagg ttactactac
     4561 tccaaatcat tgccctttta tttccattga cgtaagtaaa gctttgaccc attgtattac
     4621 caaaataaat aatcaggttt aagaaaaacg gtaatgcagt caatatacgc acataatagt
     4681 tttgacattt cctatatgtt agaagga
//
LOCUS       DAHHZJ010000181         4580 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.184, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000181 DAHHZJ010000000
VERSION     DAHHZJ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4580
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.184"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            144..1562
                     /locus_tag="NBL02_002561"
     CDS             144..1562
                     /locus_tag="NBL02_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HCD2573197.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            1633..3102
                     /locus_tag="NBL02_002562"
     CDS             1633..3102
                     /locus_tag="NBL02_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCD2573198.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            3112..3579
                     /locus_tag="NBL02_002563"
     CDS             3112..3579
                     /locus_tag="NBL02_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCD2573199.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            3615..4487
                     /locus_tag="NBL02_002564"
     CDS             3615..4487
                     /locus_tag="NBL02_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCD2573200.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
BASE COUNT         1523 a          658 c          952 g         1447 t
ORIGIN      
        1 cataaaaaaa aatacagcat aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat
       61 atttttatga aacgggttga catatgaaac gggttgacat atattagttg tgaagataaa
      121 aaccttttca ctggaggaag aaaatggaga aattattaat taaaagcaat aatcatcaac
      181 tttactacca acctaaggat gtttgggtcg gagatgttat gccttacggg aaagaaggaa
      241 aattctttat ttatcatcaa cgtgatacta gaaatccagt tccttttgga gaaccgtttg
      301 gctgggcttt agcaactacg aaagattttg ttcattttga agactttggg gaatctcttt
      361 taagagggga tgacaatgag agagaccagt ttatctacgc tggttctgta tttgaggcaa
      421 atggaaaggt tcacgcattt tatacagggt ataatcgtga attcttagcg cagggaaaga
      481 catctcaagt gttgcttcat gctgagagtg atgattttgt tcattgggaa aaatcagaaa
      541 cagcattgga gttagaacca caagaagggt atgatattcg aaactggaga gatccatttg
      601 tcgtttggga tgatgaaaaa gaagaatatt tgctaatttt aggggcgcga aagggtcagg
      661 ataaacatct acaaaccgga cgtttagtgg aatttacatc aaaagattta actaattggg
      721 aattcaaagg tgatttttgg gctccgggat tgtataccat gtttgaaatg ccagaacttt
      781 tcaagattgg tgactggtgg tatcttgtct attcagaata ctcagatgga aataaaatac
      841 tctatcgaat gagtcaaagt ttggaaggtc cttggattaa gcctaaagat gaagcttttg
      901 atggacgggc ttattatgca ggtagaacag cttatgatgg aaaacgtcga gtcctatttg
      961 gatgggtacc aacaaaagat caagaaaatg ataaaaacaa ttatttatgg ggaggtactt
     1021 tggttcctca tgaagtctat caaaaagagg atttttcact tggggtaaaa cccatagacg
     1081 aagtgtggaa tgcattcata aatttacatg atattgccga tgtagagata gaaacagcag
     1141 acacaagaga agaagtaacg cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa
     1201 caatttcatt tgatgagaat cttagtgact tttcactgag attatataaa aaccttgaga
     1261 cagatgaatc ttacgaatat cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa
     1321 acccttgtta cccttggttt caatatatgg ataaaggact tattagacct atatctttaa
     1381 agcctaacca atcttataaa ctaaaagtga ttgttgatga caatataatg acactttata
     1441 ttgatggaat cgctcttaac gctcgtgtac atggttgttt tggtcaagca attactatta
     1501 cagcaacaaa tggatcaatg actgcaataa ataccaagtt ttcaaatgaa ataaagaaat
     1561 aataaacgtc aatcatgtgg caggtctgaa aatcaaacat gacctgtcac atttaaaagg
     1621 gaggataaat gaatggacta caaaaaaatt gccgaagatg tattggaaaa cgttggagga
     1681 aaagagaata tctcaagcgc tacacactgc attactcgtt tacgtttaat tcttaaagac
     1741 aaaagcaaag cgaatatgga aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc
     1801 aatagtggac agttacaagt aattttcgga acaggtataa ttgaaaaggt ttacaaagag
     1861 ttcgttgaat tgacaggagc taaggaagta tccgtttctg aaatgaaaga cgaaggagtc
     1921 aataattcag gaaatagact tcaacgagct tttaaagttt tctcggatat ctttattccg
     1981 atcattccag cttttattgg ggcagcaatg gttttaggaa ttcgttcgtt gattaccact
     2041 cctggaatgt ttggaatgga tggctcattc gctgatcaca atgtattcct aaatgattta
     2101 tccaaatttc tgggtattat tgcaaccaca tttgattatt taccagtttt agttatgtat
     2161 tctgcaacaa aacgttttgg cggaaaccct attttaggat tacttgtagg atttgttatg
     2221 attcatccag gactagccaa tcggaatgat ttcgtcatgg gaactataaa gcctgattat
     2281 tggcatttat ttggattaag cattccagca gttgcgtttc aagggggtgt ttttccagca
     2341 attttgactt cttggttttt agcaaaagtt gagaaaatta caaataaata ttctcctcaa
     2401 gtacttgctt acattttagt cccaactgtg acaattgttc ttgccaactt tgcactattt
     2461 ttagtgtttg gtccattggg gaattttatc ggtaatgttt taggtggaat cattgatttc
     2521 ctatatatga agatgggtgc ctttggtgct tttgtttttg caggcgcgtt acaaccatta
     2581 gttgtaacag gaacacaaca tgctattcaa ggtattgagg caaacttagt cgcaacaact
     2641 ggattcaatt atatccaacc tatttggtca gtttcaatta ttgcccaagg tggcggttgt
     2701 attggtatgt atttactctt taagaaaaaa tctaaagata gagatattgc gatttcaagc
     2761 tttattccaa cactggtagg tattactgaa ccggctatct ttgctgttaa tttgaaatat
     2821 tcaatcgttc cttttgtttg ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa
     2881 atttttgatg taaaagccat tggacaagga ttgactgtat tgccaggtct gactattgct
     2941 aatccagttc ttccttatat atttggtaat ctcatagcat tcattttacc aatcattttt
     3001 attctcgctt atgataaata taaagtgttt atccctgaag ctaaacaccg taaatttgca
     3061 gaaaaaactg actcaaaaaa attgccagtt caagtaaagt gagatgagag catgttaaat
     3121 ttttttagaa aaaatgaaat aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg
     3181 gagaaattaa gtgatccagt cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat
     3241 gcaaaaaacg aaacgattgt atctccaatc gatggttttg tttcaagtat ttttcctaca
     3301 aaacatgcct taggaataaa aacaaaatca ggaattgaga tacttgttca tgtaggaata
     3361 gatacagttg agctaaatgg tgagggattt gatattaaag ttcaagaagg acaaaaagtt
     3421 aaggctgggg atattcttat gaccattaat tttgtagttt ttgaaaagaa tcacaagact
     3481 aaagatgtca ttgttgtttt tccagactac caagacgaaa ttttcattcc gaaacttgaa
     3541 gaagtaaaac aaggacaaga attgtttgtt cttggctaat gataagtaat taataataga
     3601 atcgaggaaa atgtatggtt tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg
     3661 ccattggaga taaagatttg aacatatttg aaaaagtcag ctttcccacg actacaccca
     3721 aagaaacaat gaatttagtt ttagattttt ttaatcatca taaagatgat ttagaaggta
     3781 tcggagtggg atcgttcggt cctattgata ttcattgtga atctaaaacg tatggcttca
     3841 ttacatcaac gccaaaactt gcttggcagg attttgattt tattggaagt ttaaaaaagc
     3901 aacttaatat tccaactgct tggacgactg atgtaaatgc ctcttgctat ggtgaatatg
     3961 tttctggaaa tggcagaggc ctttcaagtg ttgtttacta taccgtagga acgggcatag
     4021 gaggtggcgc tatccaaaac ggaaacttca ttgaaggttt tagtcatcca gaaatgggac
     4081 atatgcttgt aaaacgacac aataaagaca acttttctgg gacatgtcca ttccacggag
     4141 actgtttaga gggcttagct gctggtccgg caatcgaaag aagatcaggg aaaaagggac
     4201 aagaagtgac tgaaaatgac gttacttggg aaatagaagc ttattacatt gctcagtgtg
     4261 catataatac aacattgatg ttatcacctg atgtcattat atttggtggc ggagtaatga
     4321 aacaaaaaca cttaaaaaag aaagtacaag ataagtttat tgagttgatc aacggatatg
     4381 tcgctattcc cgatgttgac aattatatta tgacaccaaa attgttagat aattctggta
     4441 ttgtaggttg tttagcttta gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga
     4501 tcgtctcgac tatttgtttg gttgatgcga tctttttttt caattagtaa ttgaagggac
     4561 gcctaaaagt gaaaaaaaaa
//
LOCUS       DAHHZJ010000182         4489 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.185, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000182 DAHHZJ010000000
VERSION     DAHHZJ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.185"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            95..1429
                     /locus_tag="NBL02_002565"
     CDS             95..1429
                     /locus_tag="NBL02_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCD2573201.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            1470..2381
                     /locus_tag="NBL02_002566"
     CDS             1470..2381
                     /locus_tag="NBL02_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCD2573202.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            2378..3232
                     /locus_tag="NBL02_002567"
     CDS             2378..3232
                     /locus_tag="NBL02_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCD2573203.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            3229..3846
                     /locus_tag="NBL02_002568"
     CDS             3229..3846
                     /locus_tag="NBL02_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HCD2573204.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            3848..4366
                     /locus_tag="NBL02_002569"
     CDS             3848..4366
                     /locus_tag="NBL02_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCD2573205.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
BASE COUNT         1348 a          764 c          907 g         1470 t
ORIGIN      
        1 ttagaatcat accttataaa ttgttatttt cagaacaaaa tgatatcgtt atattaaaac
       61 cggcatcggt tacattaaaa tttggaggta aaatatgaaa aaattattgt ttacagggtt
      121 gctggcaagt tcagcattgt ttttattagg aggatgcgga tcaagtaatg caagcgatgc
      181 cagttcaagt gcagcaaatt cttctgaaaa aacaagtgat ggaagaaaaa atattgattt
      241 ctggtccttt tggggatccg gtgcacgaaa agaagctatc gaagaaatca ttgaggattt
      301 caatcaatca caaaacgaga tccatgtaaa ttataagtat cagccatggg gtgacatctg
      361 gactaaatct ttatctgcaa taacagctgg aaatgcaccc gatgttattg tacaggacat
      421 aaattccgtt gcccaaagag cagaagcaca gcaagcaact aatctctcca aatatgtaga
      481 aaaagacact tctaaggatt tttatccgca gctttgggat acagtagaat atgaaggtga
      541 tccttatgcg atcccattca atacagatac acaagtcatt ttttataata aaaagttatt
      601 taaagaagct ggaatcagtg aacaagatct accgacaact tgggatgaat tagaagaaac
      661 agcacgtaaa ctagataaaa aagaaggaaa agaatttaca cgcatcggtt tttatccatt
      721 atggaattta ggggctgatg tatgggcgct aaacgcagat aacgggacta gttggtttga
      781 caaagatgaa aaagtcaaga ttgatactaa aaataaagtg gaagccttgg agtggatcca
      841 agactggcaa gattattatg ggaaaaatac gatcaataaa ttagaagccg aatttgggtc
      901 tggtgtatct gatccattta tttccggatt agtagccatg cgtgcccaaa atatcaatta
      961 ttactctagt ttgatggaaa atgcaccgga tgattttgat tttggcgtga tgcagctccc
     1021 agaaaaagaa gaaggttcag gccattggtc gtggggtggc ggattcgtct tggaagtacc
     1081 atacggagca aaagatcctg aggcctctta tgaattcatc aaatatcttt cttctccaga
     1141 agtacaagaa aaattcggcg aaaaaagttt tgatatcatg gcgagcaaga cagccaatga
     1201 gaatttagtg aataatgaca acctaacgga taaaggaaaa atgatctatc aaatggcgga
     1261 cgaaaacttt aaaaatacgg tgatcacacc agttccttta gatgctccgg attacacgac
     1321 attagtgaat gaacagattg accagattct tttaggaaca aaatcaccaa aagaaggatt
     1381 ggcagatgca caaaaagcag tagaaaactt agtcaagcaa aatcaataat ccatttttga
     1441 ctgtctaggt acagaggagg caagaaaatt tgaagcgttt atctatccga aagaaaaaag
     1501 aagcaagatg ggcctattta tttatctcgc cttttattat cgggttcttg gtatttatgc
     1561 tagggcctat gcttttttca gttattggta gtttcaccga ttataatttg acatccaaga
     1621 tgaatttcat tggtctagca aatttcaaaa gaatgttcgt tcacgatgat ttattttgga
     1681 aatcgttata caacaccgtt tatttcgtca tcttcaatgt accgctgacg acgattttca
     1741 gtgtgttttt agccacagta ctcaatcaaa aaatcaaagg aatcagtgta ttccgtacag
     1801 ctttctattt accggcagtc ttatcaggag tagcagtcta catcttatgg atgcagctgc
     1861 tttctccatc tgcaggattg atcaacacat ttctgggctg gttccatatc cctggaccag
     1921 cttggctgtt tgatccacaa tggacgaaac ctgcgttgat tttgatgaaa atatggagtt
     1981 ctggaggagc aatgctactt tacttagcta cattacaaaa tgttcctaga tctttatatg
     2041 aatcagcaga aatcgatgga gcaggcgttt ggggaaagtt caaaagtatt actttgccat
     2101 taatcactcc tattattttt tacgatgtga tcacctctac gatcggttct ttccaaattt
     2161 ttcaagaagc ttatgtcatg acgaaaaatg gaacaggcgg acctgcaaat tctttgcttt
     2221 tttataatct gcatatgtgg aacaaggcgt tcgtagcttt tgatatgggt tatgcgatgg
     2281 ctatgtcaat gatcctgttt ctgatcgtgt tggttctgac gtttgtcaat ttgaagcttg
     2341 cacctaagtg ggtctattat tctggaggtg acggcaaatg atggattatt tcaaaaagac
     2401 cagtgtgaat cggcaaacaa aaaaaatact aagttatgtc ctcatgacag taataggaat
     2461 cattttgatc attccattgc tttggatggt gtttacttca ttgaaaccaa tggaagaaat
     2521 cgttcgttat cctccgacct ttttcccaga aaaaatcgta tgggaaaact atctggatac
     2581 gattgccgcc tttccttttt ggcgctatgc aagaaataca ttattcatca ctgtgctagt
     2641 agtgatcggt aatgtccttt caaattcttt tattgcttat ggatttgcga agcttgattt
     2701 tcctgggaaa aagctgatgt ttgctttggt tctttctacg atgatgatcc cggggtttgt
     2761 aacgatgatc cctcaatatg tattattctc caagatcggc tgggtaggta cttatttgcc
     2821 cctgatcgtt ccatcgttct tcggcaatgc gttcaatata tttctgatgc gtcaatttta
     2881 cttatcgatc aataatgaat tgatcgaagc tgctgaaatc gacggagcga atcatttgta
     2941 tatttggagt catttgatgc ttccattgac gaaacctgcc ttgattacca ttgcgatcaa
     3001 ttccttcaac gcagcttgga atgatttctt aggtcctctg ttatatatcc aagatcaaga
     3061 aaaatacacg ttacagattg gcttgcaagt ttttcagaac caagcaacga cacaatggaa
     3121 ttatctgatg gcaggagcaa cacttgtctt gatcccgacg attttgctgt tcttttttgc
     3181 acaacgttat ttcattgaag ggatggattt gacaggaggt tcaaaaggat gatgaatcga
     3241 tcgattggta aaaaagccaa tgattatttc ttagaaatca ttgaatggat tccacgtctt
     3301 atccatttac agctactatg gttcctgtgc gtcttgcctg ttttcacatt aggaactgca
     3361 agtcgaacag ttctgtatat gatttatcat tacaaaaaaa atgaagagaa aaagctcggt
     3421 tcactattct ggaaaaagtt tcgtgagaac ttctctcttt atggaaagca agatagttta
     3481 gcaagtgttt attttcttct tctattgatc gattatcaga ttttccgcta ttggggtggg
     3541 ggaattggct atacattgat gtatactacg ttgtttttgc ttctattaag cgcgcttctc
     3601 tttagttaca aaatcttgct tcaactggaa caaaaagaag cttcttggat tactgcattt
     3661 ttcttgtttt ttaatgattt caaaagcgcg cttttctatt tggcaggcac gttgatcctt
     3721 cttgtcgcat tctggtttgc aggaccaatc tattttgctt tattaggttt cagtttcttg
     3781 ttttattttc aagtcctgct ttttatccat cgtacgcaag aaaaggttct taaaaaggag
     3841 gaataacatg ttgcgtttga ttgctaaaat cgatgatagt attatcaaca agttcgccaa
     3901 gcaacggaaa acggatagtc gaaaaatcaa ttttttgaaa tttatcagta atatctctaa
     3961 tggtggtttt atttggttgc tccttgctgc tgttttagtc ttgatacctg atactagaga
     4021 atatggtcta gccattctta tcggcagttt gatctatttt tttgttggtt tgctttttat
     4081 caaaaaaacg acaagacgga aaagacctta tgaaaaaaat cgtcggcttg tcctttacac
     4141 aagtaaaccg aagacttctt cttttccttc aggacataca gcgctagcct ttttatcagt
     4201 agctgcgttg ttcgaattaa gtccagttgc aggaatatta gcctttttat ttgcttgttt
     4261 gatcgcttat tcgaggattt ttttactttt acattatcct actgacattt taggaggagc
     4321 tattttagga agtgttattg gcatcctatg cgctgccgtt atctgaatta attgtacgaa
     4381 taaagcatga gtgcgtaaat gcgcatttgt gctttatttt tttgtttcga ggaaagcgta
     4441 gttgtttatt catttgtctt aataacttaa gttttgttaa atgagaacg
//
LOCUS       DAHHZJ010000183         4470 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.186, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000183 DAHHZJ010000000
VERSION     DAHHZJ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.186"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            113..967
                     /locus_tag="NBL02_002570"
     CDS             113..967
                     /locus_tag="NBL02_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCD2573206.1"
                     /translation="MENSIYLVTEPNTQLKDCYFNFCGYSKTEPRHSFGPAIREQYLI
                     HIVLEGEGYYSINDQKYFLKKGQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYY
                     LKEIGVGKNHLTFKVENLNSFKGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSAS
                     YLNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISI
                     KEYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRL
                     GLGIINGE"
     gene            1064..3313
                     /locus_tag="NBL02_002571"
     CDS             1064..3313
                     /locus_tag="NBL02_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCD2573207.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3316..>4470
                     /locus_tag="NBL02_002572"
     CDS             3316..>4470
                     /locus_tag="NBL02_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD2573208.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTR"
BASE COUNT         1575 a          632 c          816 g         1447 t
ORIGIN      
        1 aggaactttg agatattctt taggatatta aaattaacta gcaccacggg ttatgtaata
       61 atataaacat aataaaacaa aaattatcca aatctaacat aggagggtgg atatggaaaa
      121 ttctatatat ttagtaacag aacctaacac tcagctgaaa gattgttact ttaatttttg
      181 tgggtattca aaaacagaac ctagacatag ttttggacca gctattcgag aacaatactt
      241 aatacacata gtgctagaag gtgaaggcta ttattcaatt aatgatcaaa aatattttct
      301 gaagaaaggc caaggatttg ttatcccacc aggagtatct actttttatc aagcagatga
      361 aaaaaatccg tggtcatatg tatggttggg tttaagtggc aatcttgtag aatactatct
      421 aaaagaaatt ggtgttggaa agaatcatct tacctttaaa gtcgaaaatt taaattcatt
      481 taaaggatta atttttgaaa ctttatcgta tgatcaggac aatctagtca gtgaattgtc
      541 cagacaaaaa caagtttata aatttttaga actcctctta aattcagcta gttatctgaa
      601 cgaagggata attactaaaa agatgaatcc ttttgttcgt caaacaattg aaataatacg
      661 tgagaatgcg tatcaaaaat tatctgttaa agatattgct gtcagtctat cgatacacga
      721 aaattattta tcacgattgt ttaagaatga tatgggaata tcaataaaag agtatattag
      781 tgaaatcaaa atttctattg caaacgatct gttatcatca actgaatatt caatccagca
      841 aatcagtgac aagataggat tctcaacagt acaatctttt tctaaagcct ttaagaaagc
      901 aaatggtgtt tcaccgggag tatatcgaaa acgtagatta ggtctaggaa tcattaatgg
      961 tgaataaact aaacatttga ttttgataaa tattcgttac tttaaacgct tactatttat
     1021 gtgagcgttt tttataatgc aataaagaaa cggaggagag cttatgtata aggataatga
     1081 aatgaatatt atttatcgat cagaaaacca acaatttcat ttgtttaatc aggagataag
     1141 ttacattttt aagatatcgg aagatggtaa attattgcat ttatattatg gtgagaactt
     1201 accagataat gactatagtc atctaattga gatgcatcat cggcctatga caacctatag
     1261 aaatgaaaac gatttgctat attcgttaga gcacttaaaa caagaatttc ctgaatatgg
     1321 tacaacagat tttagacatc ctgctatttc attactacag aaaaatggct ctcgcttatc
     1381 agattttgtc tatcaagggt acgaagtgga agaaggaaaa cctaaactag aaggtttacc
     1441 tgctacctat gttgataata atgatgaaag caaaacattg aaagtttata ttttagattc
     1501 agttacaaat gttcaagtag aattattata tacaatttat agagattatc ctgttattac
     1561 tcgtagcgcg cgagttatga ataaaggaaa agatattcaa ataatcgaga atattagcag
     1621 tttatcattg gatttacccg atgcaaatta tgaatggcaa caattatctg gtgcttgggg
     1681 tagagaaaga actattaagt cgcgtacttt acaacaagga attcaatcaa tcgaatctac
     1741 tcgaggtatt tcaagccatc agcataaccc atttgttact ttaaaaagaa aaaatactga
     1801 tgaaaatcaa ggagaagcta ttggtgcagc actagtttac tcaggaaact tcttaattca
     1861 agcagaagta gatacatggg atgttactag actacaagtt ggcatcaatc catttggatt
     1921 taattggaaa ctcaatccag gagaaatatt tacagcacca gaagcgattc tggtttattc
     1981 aagtaagggt ttaaatgcca tgagtcaaac ttttcatcaa ctatttagaa aacgcttagc
     2041 tcgtggtaag tggcgagaaa aagaccgtcc tgtcttaatt aataattggg aagcaacata
     2101 ttttgatttt gacgaagaaa agttggtaaa tattgctaaa aaagcatttg atgttggtat
     2161 tgaattattt gtattagatg atggctggtt tggagaaaga gaaaacgatt gtgcaggatt
     2221 aggagattgg catgtaaatc ctaaaagact acctagagga atcggcagcc tttcagaaaa
     2281 aattcgtagt tttggtatga aatttggttt atggtttgaa cctgaaatgg tgaataagga
     2341 tagtgagtta tatcgcacac atccgaattg gattatttct acacctaatc gaaaacctaa
     2401 tcatggtagg aaccaatatg tattaaattt tgggttagat gaggttgttg aaaatatttt
     2461 cgatcaaatg tgtaaaatca ttgatgaatc aaacttagat tacattaagt gggatatgaa
     2521 tcgtccattg actgatgtat ttgacagtca cttgtcagca gatcaacaag gagaagtatt
     2581 ccacagatat gtactaggcg tatatcgctt atatgaaaaa cttataacaa aatatcctaa
     2641 catactattt gagtcttgtt catcaggtgg tgggcgcttt gatcctggaa tgcttcacta
     2701 tgcacctcaa gcttgggcta gtgatgattc agatgcaatt gaaaggttaa aaattcaata
     2761 tggaactagt atgctgtatc ctctttcgtc tattggtgct catgtatcaa ttgttccaaa
     2821 tcatcaaaca aaccgtataa cacctataaa aactagagga aatgtagctt tttttggtgc
     2881 atttggatat gagcttgact taaatgaatt aagtgaaggc gatttgagga ttgtaaaaga
     2941 acaaattgcc ttttataaaa aatatagaac cataattcat aatggtactt tttatagact
     3001 attatcacca ttcgatgatg ataatcaaac cgcgtggatg gttgttagtc atgataagaa
     3061 aacggctatt gttgctcatt ataaaacatt aaatgaggta aatgctcctt accgccgact
     3121 gaaattaaaa gggttagata atgacggact ttataatgta ggacaaaata gtttgcgatc
     3181 tggaatggaa ttgatgagag caggactagt ctgttcagat gcatctagtg gacaaataaa
     3241 tgatcagaat cgacgcccag aaacatttga ttttgattcc aagatttggg ttataacaca
     3301 ggaggaaaag tagagatgaa taaccaaaaa agcttacttc aaaaaggagc aatcttaagt
     3361 atttcactaa tgttaacaag ctctcaagca attaatggtg ttattccaca aatgaaacag
     3421 tctttgggga tatcacaaag tcaaagtgaa cttttaggaa ctgcaccaag tataactgtt
     3481 attttgttta tcttattatc tagttacttt gccaaaagaa taggaatgaa aaagacaata
     3541 attttaggtt tattattagc tggtatagga ggaattattc cagtatttac agctaccttc
     3601 caaaccgttc ttattagtcg agtaatatta ggtgccggtt taggagtata taattcattg
     3661 gctgtgacat atatcagctc tttatactct ggcaatacta gagcaacgct attaggtatt
     3721 agaaattcaa tggaagcttt aggtcagaca atccttattt ttcttgctgg agttttagtg
     3781 aacattaatt ggacggcttc gtttttagtc tatgcaattg cgtttcctgt agctctctta
     3841 ttcgcgctaa aagttcctga aattagagat gaaaatagtg acatacctga aaatcatgta
     3901 aaagaaaaga tgaatccaat ggtttttgcc ctagttctat ttgcaatctt actcgtaatg
     3961 aatagtattg ctatttctgt acgatttgca tcaattgcaa ctgaaattaa aggggtaaac
     4021 tttaatgcta gtaactattt agctctaatg cctatcttag gaatactagc aggatttatg
     4081 tttggaccaa taaataagtg gataggaaaa ggaaccctat acttgggaat tatattcttt
     4141 ataatttcaa atttgttaat tgctgtgtca aacggtaata tgacgttcct gctaacgggt
     4201 ttatttttat caagtataac aggatcatgg tgcttcccat ttatctttag taacttagat
     4261 aaagtcacta caaagaatac aattaatttt gctacttcat tgatttttat tggctgtaat
     4321 attggtaatt ttatagctcc tattgctatg caattagcac aatttctaac aagaagtaca
     4381 aatttgactg ctccattttt tgtattcgca ggaatattta ttgtgatcct atttgtaaca
     4441 ttttttgcga caaataaaaa aaatactaga
//
LOCUS       DAHHZJ010000184         4426 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.187, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000184 DAHHZJ010000000
VERSION     DAHHZJ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.187"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..125
                     /locus_tag="NBL02_002573"
     CDS             <1..125
                     /locus_tag="NBL02_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCD2573209.1"
                     /translation="NQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA"
     gene            complement(78..314)
                     /locus_tag="NBL02_002574"
     CDS             complement(78..314)
                     /locus_tag="NBL02_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD2573210.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFLMILFVKLSETKLSIYVTQKNF"
     gene            complement(734..1855)
                     /locus_tag="NBL02_002575"
     CDS             complement(734..1855)
                     /locus_tag="NBL02_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HCD2573211.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVMDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYKPG"
     gene            2183..3592
                     /gene="istA"
                     /locus_tag="NBL02_002576"
     CDS             2183..3592
                     /gene="istA"
                     /locus_tag="NBL02_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HCD2573212.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3589..4317
                     /gene="istB"
                     /locus_tag="NBL02_002577"
     CDS             3589..4317
                     /gene="istB"
                     /locus_tag="NBL02_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HCD2573213.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
BASE COUNT         1465 a          801 c          829 g         1331 t
ORIGIN      
        1 tcaatcaagg attcatctca tccgttgcgt ccaaaagaaa ccatatccct agaaaatcac
       61 taaattacca aacaccatta gaagtttttt tgagttacgt aaatggaaag ttttgtctcg
      121 cttaatttga caaataatat cattaaaaag ttagtaaata attgccctct aagttccacg
      181 gtctgttcat taataacttt atatcctcgt ttttcaaaaa atggctttgc ggtaatggaa
      241 gcgtgggtag tgattttttg cataccagtt tctaaaacat ggttttccaa ttcatctgca
      301 attttagtcg caataccttt tccttgatgg ctatgttttt gtcacaactt cagtattcct
      361 gtaacaatac ggatttcagt atacaacaac agcttttgaa aagatacttg caaaaggatt
      421 aagatatcta tgacctgcta tgctcactga tgttgaaaag aatgttttat taggattgag
      481 gtaagcaaga tagtgaatat gtccggaaag cctgtaaaat aaggcttttc ggacatatgt
      541 gaagttataa aaagatagcg aaaaatctat ttagtatcac taatgttgcg aacatatcgt
      601 cagaatagtc tgacatttat ttcttgctaa aaatttcaaa aaagtcaagt gctgacgcag
      661 cggatgacta ttttccagaa aattttagta tgtattcaaa aatcaaacgt gacaacagct
      721 attaactaca tcctcatcca ggtttgtaac gtttgaacca ctcttctgcg ggctcaaaca
      781 ttaagtaatg gaaggtacgt ttcaattgaa acagcgtcgc tgtcgtattc aactcaattt
      841 ttatcacata agtgagaagc gttgcgatta aggtgaggat gacttgattt atcgcccctt
      901 gttcactatg tgaatatagt cgtttaatgg tcagattttg ttttaagtgt ttgaacaaca
      961 attcaatctg ccaacgggct tgatacatgt cggccacttc gtttggcgat acatcaaaga
     1021 gattggtcac cattcgcagg attttttgtc ccttggtaag aaccgttacc aacctaaaac
     1081 gctctgtcac atggttgaca ccgccgacaa gaatcacttg ttggtcactg attatcgtcc
     1141 catcacgggt tgttgtatct gctacttcaa tttgatccaa gatggtaatt tttgtattag
     1201 attttgtccg tgtaacgaag aaatagccgt cacgatttag tttgtctaac aacttgtaat
     1261 caaagtagcc gcgatccatc acatacgttg cttgcgtttt atcaacaaga acttcaaggt
     1321 gattacggtc atgctcactc gcatttgtca tcgtaaaact ttcaggatac tgattggttt
     1381 tatcaagata acagagattc aaatgtagct tgatgccaga ctttgttttc cgaaacttgg
     1441 cccatggaaa ccatgctttg ttcaacggaa tagtcgttga atcaatcaat tgtagactgg
     1501 ttgtttttga gcttggctgc tgagcagaaa ttctggcgac taattgcgta aaaatttcca
     1561 tcaatacttc ttgcttgatt tctggcgctc tacgagataa cgagctgtaa cacagggaat
     1621 caatgccaag ttccttgcag aggcgctgat ctaaaaaggc gcgatcaatc tcgcgatagc
     1681 ttggcaattc ttcataaacc gcatgaagta acactttaag cgtcgtttgg aagtctagtt
     1741 ttttactgta gaaatcaaag tcgctaatta agctttgaga gttttctgat aaaccagaaa
     1801 aatttattgc agaaaaccat ttttgaaatg aggtttttac gttatactta tccatgcgtg
     1861 agtccttttt attggtcttg gataagtcat ccgagaatag tataaaggac ttttttgcgt
     1921 ttggatagct ttgtattagg cttttgatgg gttaaaaagt ctttgcaaca ctagtaattt
     1981 agtatttatg tataaattga aagatatttt accaccactt agtcttctta aaagtgatcc
     2041 aacattttgt ttgtggcaga atttaactac acactaatgc agccgaaagt acacaactat
     2101 gcagcagttt gactgcgaac tggtttcctt ccatcataag tttgaatgac ttagtgatgg
     2161 aggtacagaa aggttgttaa ctttggatga tattaaaaca ataaaacatt tgagaaataa
     2221 tcgtgatcag tcaattgatc agataagaaa agcaatgaat atcaattggc gaacagcgaa
     2281 aaaatacgcc gatagtgatc aactattcat tacagatccc actcgaaaaa acaaatccat
     2341 gatggatgat ggttggggag aaatcatcgg tagttggcta gaagaagacc tacgtttacg
     2401 cagaaaactt cgtagaacgg ccaaagctat ccatgaacaa ttgaaacctt tgggtttccc
     2461 tggctcctat cgaacagttt gtgtatacat ttctcaatgg aaaaagcagc gttacgacga
     2521 aacgaattac caagctgtcg atcgtcttga tcatccgatc ggagaagcac aggtcgattt
     2581 tggaacaatg gaagttatgc atgaagatca catgaaagat atacaacttc ttatcatgtc
     2641 atttccctat agtaatgcga cgtccgcaag tgccatgcca gctcaaaacc aagagtgttt
     2701 cctggaagca ttaaaacaac tatttaatca atgcggcggc gttccaaaaa cgatcagaat
     2761 agataactta actccagcgg tgaaaaaaac aaaatcaaaa tttgaagaag cacaactaac
     2821 cgatgaattc ttacgtttca ccattcatta tggttgtgat gttcaagtct gtaacccacg
     2881 tagtgggcat gaaaaaggac atgtcgaaaa taaagtgggt tatgtccgat acaatttctt
     2941 ctcgacttcg ccaatcatgg actcttttga gtccctaaat aagcaacttg aagaacaatt
     3001 aaaagcagat cgaaaaagga cgcattattc aaaacatcaa ttaatcgaag atttgtggaa
     3061 gaaagaaaat aaagcgttac attctcttcc acaagaggat tacccaattt ttaaagaagt
     3121 ggaagctaaa ctaaatcgtt atcacgaaat gaaattggat caaacggtta ttcatattcc
     3181 taaagcatat ggctattctc aattgactgt tgttttgact tggaataaat tcaaagttct
     3241 tacgcctcat ggagaaatat taatgaacga atatcgcccc tatatgaaca aaaaaagatt
     3301 gatcccatgg agtgacatca tgaaacaatg gagtaataaa ttgaatgtcg ttagttattc
     3361 gaggtattgg aagtacttgc ctgcacgcct tcaaaattat ctagcgattg ataataatca
     3421 cctgaaatac agacggataa aacaattact aagtttatta gcgacctacg atatccaaca
     3481 aattaatgaa caattttacg aattagtcgt tcaagaagac gccccctctg actttgatgt
     3541 gaattggtca aactatgatg cattaacaaa aacaggaggt gtgcaaaaat gattggacag
     3601 acgttagaag attattgtaa aaagcttcga ttaagccata tcatcacgaa cataccaaga
     3661 gcttctttcg aagctccaga acaatttatt accgatttat tccgtttaga actggagacc
     3721 agacagcaga tcaaattaga acggatggaa aaattagcca aatttcctgt accaaaatcg
     3781 ctaaagaact atgaatggca cgaagatatt tttctaccat ctcatctaga taaggagacc
     3841 cttgtctctc ttgattttct aaaaaggcaa gaaaacattg tttgtgttgg ctctcctggt
     3901 acagggaaaa cacatctagc gatcgcatta gggagaaaag cctgtagtga aggtgtttca
     3961 accaaatttt tccgagttaa caatctcgta gagcaattag agaatgcatt aaaaaaacaa
     4021 cagctagcca gtttcaagaa gcgattcgaa acaacagaat taattattct tgatgaaatg
     4081 gggtacttac cattgacaaa agaaggagct gaacttctct tccaactgat ttcagattgg
     4141 tatgagcaaa agagcattat tattacctct aatttagaat tcagtcaatg gaatcgagtg
     4201 tttatggatt ctcgattaac agctgctctc gttgatcggc taatccatca cgctcacatt
     4261 atcagcttca aaggcgaaag tttcagattg aaaaattcat tatctaaatc atattgaagg
     4321 aaaaaactag ttgcagcaaa actctgcact ttcggctgca tttttttgta aaaagctatt
     4381 gcacaacaca aacatttgaa cgacgtaatt ccaaatgttg gattgc
//
LOCUS       DAHHZJ010000185         4420 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.188, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000185 DAHHZJ010000000
VERSION     DAHHZJ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.188"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(42..653)
                     /locus_tag="NBL02_002578"
     CDS             complement(42..653)
                     /locus_tag="NBL02_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCD2573214.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(776..901)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(983..1699)
                     /locus_tag="NBL02_002579"
     CDS             complement(983..1699)
                     /locus_tag="NBL02_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCD2573215.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(1705..2274)
                     /gene="scpB"
                     /locus_tag="NBL02_002580"
     CDS             complement(1705..2274)
                     /gene="scpB"
                     /locus_tag="NBL02_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HCD2573216.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(2258..3055)
                     /locus_tag="NBL02_002581"
     CDS             complement(2258..3055)
                     /locus_tag="NBL02_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HCD2573217.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(3025..3396)
                     /locus_tag="NBL02_002582"
     CDS             complement(3025..3396)
                     /locus_tag="NBL02_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCD2573218.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(3415..4302)
                     /gene="xerD"
                     /locus_tag="NBL02_002583"
     CDS             complement(3415..4302)
                     /gene="xerD"
                     /locus_tag="NBL02_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCD2573219.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         1199 a          970 c          774 g         1477 t
ORIGIN      
        1 ccaaaaaaaa agaccaagaa atttcttgat ctttttgata tttatttgac tgctgtatga
       61 cgttgttcga actgatgttg ttttcgactt aaccatggaa gaagttgcgc atgcagcact
      121 aaaaagacag cactcacgat gaatcctttg atcaagttga aaggaacgat cccaatcagt
      181 acatagtttg ctaaagacat gcccagcatc tgtgttgcgt tcagccctaa aaacattaag
      241 taaagaggcg taataacgaa ataatttgcg atactcataa aaatagttag agcgatggtt
      301 cctgtaatga cacctaaaaa tttgtttctt cctttatgct gtttctttac actaaagaaa
      361 taataaatcg ggaatgtaaa gatcgttgtt gataagaaac tagcagtgtc tcctaccaaa
      421 ttatccggag aaaaacctgt agtcagcaga tgtaatactg aacgcaagaa cgctgttgca
      481 actccggccg ctggaccgaa taagaacatg ctgatcaata ccgagatatc actaaaatcg
      541 atcttcaaaa aactaaaagc cggcatgatc ggaaaagcaa aatactgtaa aactaatgcg
      601 atcgcagcaa acatcgctac tgcaaccatt ttctgtacac gggtgttctt catttgaaat
      661 cctcctgtta ggactcctct tcagcgaact ttttgacgtt tctctccaaa tgaaaaaact
      721 ctattttcca gggaaaatag agttagagtt atttgatcaa gcgtcgtcaa ataagctcta
      781 tcttctttat ccagactata ctgtcggtat cggaatttca ccgattcagc tatttttttg
      841 caaaataggt cgtggactat aaccaccggt cgggaatttc accctgcccc tgaagacgaa
      901 ccttatataa tttttgttta catattcatt atacatatct attcaaaaaa cgcaacatct
      961 aacacttata aaaagtaagt gttcattttg agtggttcag caaactgctg acttcttttt
     1021 tgttcagttc acggtaatcc ccaggacgta ggccttgtaa agtcagatct ccgtattttt
     1081 cacgttttag tttttgcaca ggaagaccaa ctgcttgaag catatttttc acttgatgat
     1141 tacgtccttc gtggatcgtc aattccacga tactgtggtt tgtcttagga tccactgata
     1201 aaatttggaa tttagctgga gatgtttttc gtccttcgat ctttattcct tttgtcaaag
     1261 gtgcaagcat cgattttgta gcgatccctt ttacctttgc aacgtaaact ttatccactt
     1321 catgttttgg atgtgtcagg cgctgagcaa aatctccatc atttgtcagt agcaagatcc
     1381 ccgaagtatc gtaatccagt cgacctacag gatagatacg ttcttttacc atcggcaaaa
     1441 gatccgtaac aacttttctt cctttgtcat ctgcaacagc agaaataact cctttaggtt
     1501 tgtacaataa atagtaaccg tattcttctt gataaattgg gacttcatcc acttccacag
     1561 tgtcatgttt cccaactttt atccctaatt cccttacaac ttgtccgttt acttttacac
     1621 gtcctgcaag gatgaattct tctgcttttc gacgagaagt gatgccagcg tgggcaatta
     1681 ctttctgcaa tctctccatt acaattattc tcctttattc attccatcaa agaatagatc
     1741 tggtgacacg tcttcttcaa gtgaaatttc catttcttgt gtatcaggca gttcttctaa
     1801 tgatttcaaa ccaaaataat ctaagaagta gccagttgtt ccatataata tcggccgacc
     1861 tggtccttct actctacctt tttcttcaat cagtttatgg gcagctagac gctgtaccgc
     1921 tcctgaaacc tgaactcctc gaatctgctc gatttcagct cttgtgattg gctgtttata
     1981 agcgatgatt gctaaggttt cgacggcggc ttgagataat gtgtttgatg tcccttgcgc
     2041 atatcgctta agtaagggag ccagctcttt tttagtcgtt aatacataat ggctttccgt
     2101 ttcaaaaata tgcaaagctg agtgcgagtt ttgagtgtaa gattctttta attctttcac
     2161 taaacttgtg acttctgctt cgctgagatt taacagtccg cttaattctt cattactgat
     2221 cccttcctcg ccaacaataa aaagaagggc ttcaagttca cttaatttcg tcatgttcgg
     2281 tatctcctac tgctggataa agcataattg attcatagtt ttctttttgt tgcgcaatga
     2341 tctgcccttt tttcatcaat tctagcaaag ccataaaagt agtgacaact tcacttttag
     2401 aaggtgactc aaaaaaagaa tcgaaagtac agccatgcgc atttcttttt atacgctctt
     2461 cgataaatac cattcgttct tcgatcgttg tgtcatcggg cgtgatcgtt ttttccactt
     2521 tttgagaaaa tttcttacgt tctaagattt tctgcatggc acggatcaaa tcgatttttt
     2581 tgacttgtcc tcgcttcaac ggcttttcag catccgctaa cccatcgatg ttcataggtt
     2641 cttttgtgta ataaagactt cgttcctctg ctttttctgt cagtctttca gctgcatatt
     2701 tatatttacg gtattctaaa agctgagtga ccaattcttc tcgaggatct tcttcccata
     2761 attcttcatc gtctgtgccg aattcttgga caggaagcaa tgtcttgctt ttgattgcca
     2821 tcaatgtagc agccatcact aagtattctc ctgcaacagc tagctcgagt gttttcattg
     2881 tatggatata attcatatat tgctccgtga tcgtagcgat cggtatatca tatatatcga
     2941 tttccatttg ctgaatcaga tgaagcagca gatcgagagg accctcgaat acatctaatt
     3001 taatattgat ctccgtcaac tcattcactc tttcttcttt ttctttgcta gccattttcc
     3061 aatgtaaggc tctgtgattt cagttgcacg gatggagaca tccgggtact tttcctgtaa
     3121 ttcatctaac atttgaaagc ccatattctc accgcgataa gacggattca aagaaatgtc
     3181 atggatgatc agctgatcgt tcgttgccca gtaaatacca ataatccctt gaatgttggt
     3241 tgaaccttct ggcgtaaaga aaaaaagttc ataatcgtct tcagattcgt acgtatcaat
     3301 ctccttcaac aaagaagttt tacctgtcag tttcttatgg aaactcaata gtcccatagc
     3361 aattttacgg ttttctttcc ggtaatgtgt taacatcttc tcaccttcct atgactaagc
     3421 ccttggaaaa tgctgcttat aaacatccgt cattcttttc ttcgtgatat gtgtataaat
     3481 ctgagtcgta gagatatccg cgtgacctaa caattcctgt actgtccgca aatcagctcc
     3541 attttctagc agatgtgtgg caaagctatg ccgcagcgta tgtggtgtta cttctttgta
     3601 aatcccagcc tctcgtacta actgcttcag atttttccag attccttgcc gagaaagacc
     3661 tttcccatga ttattgacaa atacatgcat ttcagatgcg tcctttacaa gtaatggacg
     3721 tgcttcgtct aagtagcgtt ccagccattg gatcgcataa tcacctaaag gaatgatccg
     3781 ctctttatct ccttttccaa ctgtctgtac cagtccaaga gaaagatgaa gatctcccaa
     3841 tttcaatcca atcaattcac tgacgcgcat cccagtagca tacatgactt ccaagattgc
     3901 acgatctctt atccctaacg tttttgtcgt atcaggtgct tcaatcagct tttctacttc
     3961 tgtcagagtc aatgtacttg gtaatttctg tgctttcttc ggcgtatcga tatgttgcat
     4021 cggatcatga tcagtgatac gctcttgccg taaaaactga tggaatcttc tgagacttga
     4081 gatcatccga gtaacagttg ctgtcgcttt tttttcttca tgcaacttct ccagaaaacg
     4141 gatgactaaa aagcgatcga catcctgcca tgaagcaatt ttctgctcct gtagaaaatg
     4201 caagtactgt tccaaatcac gttcatagct ttgtcgtgta ttggcggaca atccccgttc
     4261 aatattcaag tagtggagat aatcgttgat ttgctctttc ataataatcc ccctcatgcc
     4321 tctctatcat atcagatacc gcttcattct gatagtcttt ttccataatc tgagcaaaga
     4381 aaaagccgtc tagacactca gacggactgg tttctttttt
//
LOCUS       DAHHZJ010000186         4345 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.189, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000186 DAHHZJ010000000
VERSION     DAHHZJ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.189"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(65..961)
                     /locus_tag="NBL02_002584"
     CDS             complement(65..961)
                     /locus_tag="NBL02_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCD2573220.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(954..1634)
                     /locus_tag="NBL02_002585"
     CDS             complement(954..1634)
                     /locus_tag="NBL02_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD2573221.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(1635..2417)
                     /locus_tag="NBL02_002586"
     CDS             complement(1635..2417)
                     /locus_tag="NBL02_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2573222.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(2410..3327)
                     /locus_tag="NBL02_002587"
     CDS             complement(2410..3327)
                     /locus_tag="NBL02_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD2573223.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(3491..3814)
                     /locus_tag="NBL02_002588"
     CDS             complement(3491..3814)
                     /locus_tag="NBL02_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573224.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
BASE COUNT         1276 a          948 c          755 g         1366 t
ORIGIN      
        1 ttttctctat taaatatata taagataata catttttcta ctagttctca ctaatcttgt
       61 ataattaaac cctccgcaac ttgacggaga tggagaagat gcccccgtct aatctagcgg
      121 tcatctctcc tcccattcct tccaccaatt tttttgacag gtaaagcccg attccgctag
      181 attccgtatt tgtgcggctg gtattttcgg tataaaaacg ctggaagact ttttcaacag
      241 aggtttgcgt tcctggtttg ataggattgg ccactgttaa tcgaatgaat ggatcctctt
      301 ttgaaaatgt cacctttgct tgtccatctg cataacgcaa taaattctgt atgatatttt
      361 gcagcactcg cagcaacatg agcggatctg ctaatacagt tgtatttatc gagtgttcag
      421 cgaacgctac ctcgatattt ttctcagaaa attgttcata aaaagagaaa aatacttctt
      481 ccacctcttt agaaaaagaa agcgtatgga gatccatttc tttttgcttc gtctctattc
      541 gagcaagatc ataaaactga tccgtcatac tgagtaatct tgcaacagat tgtttgatga
      601 tcgttaaata cttctcccgt ttctccgtat cctctacctg ttccagtaat tgtacataac
      661 cgttaatagc agtcaaagga gttctgagat catgagaaag gccgacgatc gatagtctga
      721 tgttttcttc catttcttga aatgcttgtc ctttttgttc attttcatcc aaccatgtat
      781 tcagtgcatc tactaggtcc attagattct tttctcgaaa ttcgactgaa agacgaccac
      841 catattcttg tcgttcagaa agagtggcga ttttcttagt taatctcttg atttcccttt
      901 gcaggaagaa aataaaactt cccataaaga taacaacgag aagcaacagc catttaatca
      961 aatctaaacc ccactcccca caccgtatga atatacacag catgtgcctt tgctagcttc
     1021 cctcgaagat tggaaatatg catattcact gttttctcac tatcaaaata tggttcttgc
     1081 caaactgttt cataaagatt tgctcgcgaa aatacttttt tcggatgttc taaaaaaagc
     1141 ttcagcagat cgaattctct tcctgtcaaa tggacagcca caccattcac atttacatcc
     1201 ctcgactctt tgtccagtaa gatttccttg taatagatgc gttgcgacag atcttttact
     1261 tggacagaag tctgatgacg aagttggatg ccgattcgta acaaaagttc ttcgatatca
     1321 aatggtttcg tcacatagtc atccgctcct gctgccaaca aggaaacttt atctctttga
     1381 tcgctttttg ctgaaagaac gatgatcgga acattcgatg tctgtcggat ttctgctaac
     1441 aatgcttcac ctgaaagccc tggaagcatg agatctagaa ggatcaaatc gacaggatat
     1501 gtgcttaaca ataattttcc ttccgtacct gaataagcat caagaagttt gtatctctgt
     1561 tcaagaacct cctccagtaa ggaatgaaca gcttcatcgt cttcgataat caaaatagtt
     1621 tccagcagga ccacctactt aatgtcagat ttttggaaaa taaacattcc tagagctgat
     1681 gataccacga ttgttactag actgaccaga atcattttcg gtgcttgcga ataagttgct
     1741 tgttgcgcaa agtccgtata catgagccac gtttccgtaa accactccgt tagaaattcc
     1801 aagcctttga tttttgcgaa aacaccggga atgaaggccg ttcccatgat ataaagaaaa
     1861 gaaagaagca aactgacact ggtacttcta gtgatgaatg tcaaagccac tggaacagat
     1921 aatgtcccta atgtcagcag aagcaataca ggaaatactt ttagaacata atttccatag
     1981 tttttgattc cctcaacttc accaaatccg aaagcaagtg tgtaagcaag catggctagt
     2041 cccgtgatca aaaccacgcc aagtgttgtg atcaaaagcg taacgagaag tttcgacaca
     2101 taataagtca ttcgattccg tcctaaagat aacgtattgc gaatcgtacc gttttgaaat
     2161 tcttccccaa tataaaagca cgcaaatgct gcgaaagcca gaagaatcca tgcagccatt
     2221 ttttctacca ttaaaatccc tgttgcaccg ttgatgactg tatccgttgc ttggtttaat
     2281 tcattgccgt attttgtatg catataccat tcgttccaag aataaaaaag cggaagtacg
     2341 accatcagtc ctagtgagac ccacatttta cggctcttcc atacttttat tttttcagca
     2401 gttaatagat taaacatgtg gattgcctcc agttaattgt aagaaataat cttccagttt
     2461 ttgcccaact aggtgaatac catccacagg aacttttgct tctaccaatg cttgattgat
     2521 ggctgctact tggtcaattc cttcaaaaag ctggataaca cggctagatt gtacaaaata
     2581 atccctataa ccaagagagt cgagaaccgt tactgcttgt tccgtcgccg aagtgtcaat
     2641 ttttataaat tgacgagatt cttgtgccaa ttcttctttc gataattctt tgatcaagtg
     2701 tccttcatgc aaaataccat agtgagtggc gatctgtgac aattcatcta gcaagtggct
     2761 agaaagcaag acggtgatgc cccgttcttt taccaaacgt tggatgattt ctcgcatttc
     2821 tgccatccct gctggatcca tcccattcgt cggttcatcc aaaatcaaaa attctggatt
     2881 cgtgatcagt gcatttgcaa tagctaaacg ctggcgcata cctaaagaga aattctttgc
     2941 tttctttttc cctgtatttt ccaatcgcat cattttcagc aggtcttcga tttctctatc
     3001 cgacacaccg ccgttcgctg cttggatacg taggttatct cttgcagtta attctggata
     3061 aagagcaggg gtttcaatcg attgacccat acgtcgtctt gctgcctgca aatccgtcgt
     3121 tccaaataat tcgatcgaac cttcatcaat cgagatcaaa cccataatag ttctcataaa
     3181 ggtagactta cccgctccat tttccccaat caatccatag atcattccac gtttgatttc
     3241 gattgatact ttatctaatg ctttggcagc accgtatttt ttggttatac cagttgcttt
     3301 caagaccgtt tcatttccaa aatccatact tatccttctt tcttattgat gagtgaagta
     3361 tagttgacgg gtataaagaa agcagccaga aatgtataaa gaaagtgtaa attttccaat
     3421 tgaatgtcaa aaaaaacaat cggacaatac gtgtctttcc tattatcagg cagtttgtta
     3481 tacgcttgta tcagtttcct gattctttct tttcgaccaa agtcgtttct ctgtattcct
     3541 cttcactgaa aatttttgct tctttcagat caagcttgag taattttacc agaccggtta
     3601 taaggataac tatagcaaga ctccctccca gaaaaattag actgtctcgc agacgtggtt
     3661 gcgttgtctt ccaatcaaag agcgcaccaa acagtgctaa tccaagtgga ctggccacca
     3721 tggtaatcgt tgttatcgtt gacataagtc gtccttgtat atgcgttgga acttctcgct
     3781 gaagccagac catataaaaa gcaaaagaaa acattaatcc ttaaaaacgg acttatgaaa
     3841 aataatacat gccgtttggg atttacaatt agctaccaaa taagaggtta tgacaaaaga
     3901 cttttcacaa tcccttcttc cgaataaacg gtgttctcct gccaactcct cggtcacaag
     3961 tcacaatatt tatcaaacat gagaagcaat ttttgtaaat tccttcttat ttcttgaagc
     4021 tgaccacttc tgtcacaacc tcagaagcgg ataaataata ttgtttgatg atactttatc
     4081 tttttcccgt ttgtttatag atcgaatgat attatagacc aagaatccta cctattaaca
     4141 tagctaaaaa cacaaaaaag gcactttcca aattgtggaa agtgccagaa aagttaggat
     4201 taaggtaact gcgctcagca ttgcttcgcc cagtacgatt ctcgtaactc gtgaactcga
     4261 acgataatcg caattaacgt tttgagaact gtgaagcttt acgggctttc ttaagacctg
     4321 gttttttctt cgtatcattg aataa
//
LOCUS       DAHHZJ010000187         4333 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.190, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000187 DAHHZJ010000000
VERSION     DAHHZJ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.190"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            63..296
                     /locus_tag="NBL02_002589"
     CDS             63..296
                     /locus_tag="NBL02_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573225.1"
                     /translation="MDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVFVYLVCSYL
                     LKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(455..577)
                     /locus_tag="NBL02_002590"
     CDS             complement(455..577)
                     /locus_tag="NBL02_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HCD2573226.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            767..991
                     /locus_tag="NBL02_002591"
     CDS             767..991
                     /locus_tag="NBL02_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573227.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRKQLVIESMQIRNPDHFTRSMSI"
     gene            1434..1640
                     /locus_tag="NBL02_002592"
     CDS             1434..1640
                     /locus_tag="NBL02_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HCD2573228.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            1640..1891
                     /locus_tag="NBL02_002593"
     CDS             1640..1891
                     /locus_tag="NBL02_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573229.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEVLLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            1907..2062
                     /locus_tag="NBL02_002594"
     CDS             1907..2062
                     /locus_tag="NBL02_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573230.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKEKLSV
                     AVEEMIE"
     gene            2072..2344
                     /locus_tag="NBL02_002595"
     CDS             2072..2344
                     /locus_tag="NBL02_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573231.1"
                     /translation="MNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQ
                     ALELTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            2789..2968
                     /locus_tag="NBL02_002596"
     CDS             2789..2968
                     /locus_tag="NBL02_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573232.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            3027..3383
                     /locus_tag="NBL02_002597"
     CDS             3027..3383
                     /locus_tag="NBL02_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573233.1"
                     /translation="MLKTKKNSYKLKLSILSIICLLGMIISGHLYRVAYTQFIEYKVH
                     SLGLAGDRYVEYVHYSLKCSSYILLWLIFSLFELLNLIAISSYHLDKLNTKSSRYNEV
                     FKENVKLESDLNKLKK"
BASE COUNT         1527 a          675 c          836 g         1295 t
ORIGIN      
        1 gatgatctac cgttttagtt tgagaaagaa ggagtaagca tggcaaaaaa attagtctga
       61 cgatggatct attgataccc gtaattgcat ttatagacgg gatttctttg ggcgtcactg
      121 aaaatccttt ggtaaataga attgcgaaag gatcactatt tcttgtattc gtgtatcttg
      181 tctgttctta tcttttaaaa agagcaggaa aggataattc cttttggcga ttgactaaag
      241 tcacagtagc agcgacagct gttatactta cggctattct accgctttat atgtagagtt
      301 aacgggaaat cgttgaactt cttgcgaaat attgctataa tgaacatgaa aaagaggagt
      361 tgcgctaaca acccctcatt aacagcaccg attaaagaac ggtggcagaa tattagttat
      421 ttggaaataa ccgctgacct gctaaagtag acggttattt ctttttgtca tttttaagca
      481 tatttacaat caaactaatc aaagcaatgg taaacatacc aaaaccaaga atggtttgaa
      541 ttgtctcata tgcggacaaa aggcttactc ctttcataga ttttagcact tacattcata
      601 agcaccacca cctttccaga agtcgccacc gtcttcaaac tttactgtct tgactattat
      661 agcatatcta aaatcgaata aaaattctca attttagctg tttttgctat aattttagcg
      721 aggtgatcgt gatggaacaa tatattggtt tactcgataa attagggtga tcaagacaca
      781 gccattgctt gtgcgattta cattacgcac gagtcatgaa ccaatcaatt gtgtagtggc
      841 caatatagaa atagcggata aattgctgat tttggaagaa ggcaagtata ctatttcagt
      901 gacaggtcac ttcaataaac gaaagcaact agtcattgaa tcaatgcaaa ttagaaaccc
      961 tgatcatttt acgcgatcga tgagcatata aaagaagagc tgcagtccta aatgaggatt
     1021 gtagctcttt ttgttttaca tagaaacagt aaaaatgaaa aggcctgttt aggaaggcac
     1081 aagcgccttc tcaattgaaa gacctttctc tcactccaac cgccaccgcc gttcgagaag
     1141 aaaggaagtg aacgctacgc gttcactaag ataaagggtc ttgcatagca agacaaaata
     1201 gaaaaaattt cctctcacca aaaggttcgc tcctgttcgt tatctcttta ttgatttgtc
     1261 agaccggcaa caagttgcct tttatcacca aaaagcgaca aggacaaggg taaatggcac
     1321 gctgcgcgcc cttgtcttgt cgcttttcgt tgattcattc tggccaacaa atcaataaag
     1381 aaagaactaa cgaaaggaag taaaaccttt cctaagagga ggcagaatca atcatgcgaa
     1441 agaaatttga ttattggggt gttcccttca gcgaattatt cccaaactat catgcaccac
     1501 atactgtcga atgcgattgt ggagaaagag cgaaatgtat caaaagctat tgtttgtatc
     1561 aatgtccaac atgcgggaaa aaatatacct tgtcttatgg cgattatatt ttaattgatg
     1621 agaaaggaaa gaaacgctga tgaaaacaca acacaaatta ttgactgtaa aagaagtcgt
     1681 tcaaattttg gtggacaaag aagtcatttc tgaaaaagta gcagaagttt tacttattcc
     1741 gcggtactgg acgatagaaa cccctttagt agaggatgca ccgactatgc tatttgaaga
     1801 cctagtgaag attgcaggat atgaactgca ttatcaagaa aacttggaaa cttatgaaaa
     1861 cgaatattgg ttaaccaaat tagagaacta aaaaggagaa aaaaatatgg ctcaaaatta
     1921 ctatgacgaa tttgtcaaat tacctttgga taaaatggca caaaaaatgg aggatatgac
     1981 gttcctatac aacgaaacac gtgttccaaa aaaacattat aaagaaaaat tgtcggtagc
     2041 tgtggaagaa atgattgaat aaggtgtcga aatgaatctt attgcgacgt actatcgaac
     2101 actggaagaa ctgaaaaagc aaaatgcaaa atggtttttc caagccttac tatgcttgga
     2161 agtaggtgtc aaaccaagca ctattaaacc ttctgaatac caagctttag aactaacgta
     2221 tgcgaaattt atcgaaacga aaaaagccaa gactgtttca agtgaatggt tagattattt
     2281 tgaaaatatc aataagtatg gcgcatatta caccatgaaa aaggaagaca acgaaaatga
     2341 ataaggtgat ttatcaacta acaacggatt ccatatcaac gatttgaaag aaaaaaaggt
     2401 caataatcat ttataccgaa aagtgagaat gtgtgaaaca aggacaaaaa aagcctagta
     2461 actaaggaaa tggttcttat ttcgcaccaa tcgctccaac cgcccccgcc gcttaggtgg
     2521 tgcgaaataa acgggcaaag cccgataact taagagaaag aaggtgatcc atttgaacaa
     2581 gattattccc ttattgttag tactgtttgt tggcgaaata cttatgcgag atagtgcagc
     2641 tgtacagtta aacctcgctg tatttttggt aatactggtg ttttgtttga tcttttggcg
     2701 gtttgtatta gtcctcgtga gtctactatt caagatgatt gcagccatat ttttcatagg
     2761 gttatttcga tgaaaggagt gagtaccaat gccaattgca ataggaaata aacggttacc
     2821 cgtgacatta gatgaaaaga gacaaaaaga attgcagcaa ctaaagcaga agtatggcaa
     2881 aagtgaatct aagattatgt gtattgcgtt agatctattg atcgcgcaag aaaaagcagg
     2941 atttgatgta ccagcgctaa aaaagtgacg tcacttttta atttgatcta tataagtgat
     3001 atcactttta ccaaggaggg aattaaatgt taaaaactaa aaagaattcg tacaaattaa
     3061 agcttagtat actatctatt atttgtcttt taggcatgat aattagtggt catttgtata
     3121 gagtggcata tacgcagttt attgagtaca aggttcactc tttaggatta gctggggatc
     3181 gatacgttga atatgtccat tattcattaa aatgttcttc atatattctt ctttggctta
     3241 ttttttcatt gtttgaattg ctaaatttaa tagcaatatc ttcatatcac ttagataagc
     3301 taaataccaa atctagccgt tataacgaag tgtttaagga aaatgtgaag ttagaaagtg
     3361 acttaaacaa gctaaaaaaa taaaaagaag aaaacataag ttaactaaaa ttattttatt
     3421 aagaatgatg gaatataagg ataaggttac caacataacg atcattactt ctctgatatg
     3481 ctatggactt ctatcaatca gtattttgtc tatagattat ggagtaacta tactgcaagc
     3541 cttagttttg gctatgccta tgtatctatc tttgtttatc atgcatgaaa tatataagct
     3601 atttgtttta tcaaaaaatg tagaaatacg ggtaaatatt ttggtgaata gttatcaaaa
     3661 agaactcaaa gaaagaagac ggtggcggta tttattgaaa ggatgaaaaa gtaggatttg
     3721 acatactggt attcagaaag tgccgtcact tttaaaacaa tggaaaaggt ggagataaga
     3781 tgaagaaaaa acaaactaaa ctatcattag cagatattta acacagttaa ccgcaactga
     3841 agatggtgtt gttgaatttg aacaatctga aattgcggta gtagatgatc gttaccttaa
     3901 aatgccttac ttctttgacc aagcaaaggt catttgcctt tgtcactacg atggggtacg
     3961 tgattttagg cgttaaaaag tagtgattat atcaacttta cgagtaaata taaatttaga
     4021 catgaaactg aattatccaa tggaaaatca tggtttataa tctagaaaaa ttatagtaat
     4081 gattggatta tatatattct ttcgtatttt cgtacaactg tagcttgtca aaattgagaa
     4141 atttaaccga ttaggtactt tacatcgtta tttgggtaat ttctaaaaca tgaagcttac
     4201 caacgaataa actgagacta tcttattaat tgatcaatat aagaaaatat ctttgaggag
     4261 atgatgtact atgttttcgt ctatccacga ttggttaggt ggtcttttcg gcggtggagg
     4321 taaaggtttt taa
//
LOCUS       DAHHZJ010000188         4310 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.191, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000188 DAHHZJ010000000
VERSION     DAHHZJ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.191"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            375..932
                     /locus_tag="NBL02_002598"
                     /pseudo
     CDS             375..932
                     /locus_tag="NBL02_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            990..3029
                     /locus_tag="NBL02_002599"
     CDS             990..3029
                     /locus_tag="NBL02_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HCD2573234.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDQYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGRKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFINNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3138..4289)
                     /locus_tag="NBL02_002600"
     CDS             complement(3138..4289)
                     /locus_tag="NBL02_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HCD2573235.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
BASE COUNT         1552 a          796 c          852 g         1110 t
ORIGIN      
        1 tcaaattcca ttgcctaatc attaggaaaa gtctatcatg aactcgtaaa tagaatatag
       61 taataacctt gttaggtgag gctcctatac aaacataggc tgttgcccag aaacgtcgaa
      121 agtcgccaat gggtaaaaca gaaactgttg gattaaggct ttttccaata gctaagaatg
      181 ttttctttac gttgtatagt gctaaaactc ggacgatgag atgatgctag gtcagaattc
      241 ttgttcgaaa aggtttctga ccttttcttt atatttttgt gactgactta tttgatgaga
      301 caaactcgct cctcacctga caagtggatt gctgtgtgag gaggagatgc ggtgaaaaaa
      361 gatcgattgg tggaggtcgc tcggattcac ttctggttgc acaatacaac cagaaaacac
      421 aacaagcaaa actgatctct atcatgagag attcatatgt cgatattcca ggttacggaa
      481 tgaataagat aaatgcagct tactcttatg ggggagttga tttattaaat caaacattaa
      541 aggaaaattt caaatttgaa gctccctatt atgcaagtat cacatttcaa gattttatcg
      601 attgcgttaa tgaactgttt cctggtggag tcaagatcga tgcagaaaaa tctctagatt
      661 tagatggcgt atatataaac aaaggagagc aagtgatgga tggtaatact ttactccagt
      721 atgctcgatt tcgggaagac aaagaaggag acttcgggag gatcagaagg cagcagcaag
      781 tgatcaaagc tatctctcag cagctgaaag acgtaacttc aatattcaag ctacccaaag
      841 cggttgggaa attacttggt agtatccaaa cgaaccttcc agaaagtgtg cttctggatt
      901 gtggtatgga tttcctcaaa gacatatgtt agaataaaca ggtataaata gtgaaataaa
      961 ggaatgacaa gcaagagaag gaggaaaaaa tgaaaagaag tgacaagcac ggcaaaaatc
     1021 gaacaggcgc ttatattgcc ggcgcagtga tcttaatagc agctgcgggt ggcggttatt
     1081 tttactacca gcactatcaa gaaacccaag cagtagaagc tggagaaaag acagttgagc
     1141 aatttgtcca agctttgaac aagggagatt ataacaaagc tgcagaaatg acatcaaaaa
     1201 aggcggcaaa taaaagtgca ttatctgaaa aagagatctt agatcaatac cagaatatat
     1261 acggtgctgc tgatgtcaaa ggacttcaga tatcgaatct aaaagtagat aaaaaagacg
     1321 attctactta cagtttttca tataaagcaa agatgaatac ctcattaggt gaattgaaag
     1381 atctttctta taaaggaaca ttagacagaa atgatggtca aaccacgatc aactggcaac
     1441 ccaacttggt ttttccagaa atggaaggaa atgacaaagt aagcctgacc acacaagaag
     1501 cagcaagagg gaatattata gatcgaaatg gtgaaccatt agcaacaaca ggcaaactaa
     1561 aacaattagg agtcgttcca agtaaacttg gagatggagg cgaaaaaaca gccaatatca
     1621 aagcgattgc ttcctccttc gacttaacag aagatgctat caatcaggcg atttcacaaa
     1681 gctgggtaca acccgattac tttgtcccat tgaaaatcat tgatggagca acgccagaac
     1741 ttccagctgg agctaccatt caagaagtag acggcagata ttatcctttg ggtgaagcag
     1801 ctgctcagct gattggttac gtgggagata tcacagcaga agatattgat aagaatccag
     1861 aattaagcag taatggaaaa atcggtcgct caggtttgga gatggctttt gataaggatc
     1921 ttcgtgggac aacaggtagg aaattaagca ttacagatgc agacggtgta gagaaaaaag
     1981 ttctgatcga acatgaagtt caaaatggca aagatatcaa attgacaatc gatgcgaagg
     2041 cacaaaaaac agcttttgac agtctaggag gaaaagctgg atcaactgtt gcgacaacgc
     2101 caaaaaccgg tgatcttctt gcgcttgcta gctctccaag ctatgatcca aacaaaatga
     2161 caaacgggat ctcacaagaa gattacaaag cttatgaaga aaatcctgaa catccattca
     2221 tcagccgatt tgcgacaggt tatgctcctg gatctacgtt taaaataatt acagcagcaa
     2281 tcggtctcga caacggcact atcgatccaa atgaagtgtt gacgatcaac gggcttaaat
     2341 ggcaaaaaga cagttcttgg ggatctttta aaacaacgcg tgtcagtagt gatgtatcac
     2401 aagtagactt aaaaactgct ttgatctatt ccgataatat atatgcggca caagaaacgt
     2461 tgaaaatggg tgagaaaaaa tttcgtacag gcttagataa attcattttt ggtgaagacc
     2521 ttgatttgcc aattagtatg aatccagcac aaatttctaa tgaagatagc tttaactcag
     2581 atatcttgct agctgatact ggatatggac agggcgaact tctaattaat cctatccagc
     2641 aagcagcaat gtattctgtt tttatcaaca atggcacact tgtctatcct aaattgattg
     2701 cagataaaga gacaaaagat aagaagaatg tcatcagcga aacagcagta caaacgatcg
     2761 tgccagatct gagagaagtt gtgcaagatg taaatggtac agcacattct ctttctgctt
     2821 tagggattcc gttggcagcg aaaactggta cagcggaaat caaagaaaaa caggatgtaa
     2881 aggggcaaga gaacagtttc ttgtttgctt tcaatcttga taaccaaggt tatatgatgg
     2941 tcagcatgtt ggaaaataaa gaagatgatg attcagcaac caaacgagca tcggaactat
     3001 tgcaatacct caaccaaaat tatcaataaa aaattgcttt ttaagtacag taaaaggact
     3061 gtggcgttat cgtcacagtc cttttttcaa ataaacggtt ttcacgatgt ctctttcaca
     3121 acttcttttt gatttactca cacttcataa actttttttg taaaatattt gagaacaagt
     3181 ggagaaataa gtgtacttaa taaaatcacg ataactaaag gtgaatagta ctgtggttcg
     3241 atcaattgac tttgctgtcc aatttgtaaa atgatcaaag ccatttctcc acgcgaaatc
     3301 ataccagcac cgaccatcaa tgaactatgt tgagaaaaac cggcaatctg agcaccaaaa
     3361 taacctccaa gcaatttggt caaaatcgct acaagcgtca atatagaaat aaagagtagt
     3421 tgttcactaa agtcagaaaa atcaacttct aatcctacac tgacaaaaaa tactggtata
     3481 aatactgcat atcctaaagc ctctacattg tggtatactt cttctttaac ctttgtctga
     3541 ctgacagcta tcccagcaaa aaaagcacca atgacagagc taagcccaac aagatccgct
     3601 acgtaagaca ttcctaaaca aataactagc gatacgatga taatagaaga atttgcgtac
     3661 actttttctg caaatgaaag taggaaaggc gcaatccatt tgactaataa gaaaataaaa
     3721 acaaaataaa ttagctgttc aatcaaaaca agcggtaaag aagcattgct agaagcttcc
     3781 cccgttagaa aagataggct gaaacttaga actaaaacga ctaaaatgtc atctaccaca
     3841 gaagcaccca aaattgttga tccttctttt gtattcacta cgtttagttc ttttaatact
     3901 tctactgaaa tactgacaga agttgccgca agaataatcc caaagaaaat cgcttcattt
     3961 cccgaaactt ggaatgcttt gcctgtcaac cagccaaaaa caaccgggaa gatgatccca
     4021 agcaaagcaa cgaacatacc aggtcggaaa tattttttca aaagagataa atcactttcc
     4081 agaccagcta aaaacattaa taagatcaca ccaatctccg aaaagtcatg gactagaata
     4141 tcaggtttga cccaattcaa tccagcttgg cctagcaaaa caccaacaag taattgtcca
     4201 atcactgctg ggatacctaa tctccgcgag atatgtgagc caatcgttgt tgaaatcaat
     4261 atcaggcata aaataccaat aaattccatt ctcatcattc ctttgtataa
//
LOCUS       DAHHZJ010000189         4309 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.192, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000189 DAHHZJ010000000
VERSION     DAHHZJ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.192"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            61..1362
                     /locus_tag="NBL02_002601"
     CDS             61..1362
                     /locus_tag="NBL02_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCD2573236.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1389..1649
                     /locus_tag="NBL02_002602"
     CDS             1389..1649
                     /locus_tag="NBL02_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCD2573237.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2648..3883)
                     /locus_tag="NBL02_002603"
     CDS             complement(2648..3883)
                     /locus_tag="NBL02_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HCD2573238.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1256 a          690 c          887 g         1476 t
ORIGIN      
        1 acattctttg cgttgtcatg caatgaatgg ttcatatttt attgaaaagg agtggtttta
       61 gtggaaaagc tgatgaaatg ggtcgaagag aaacttgtcc caccaatggc aaaaattggt
      121 acacagcgtc atttattagc aattcgtaat ggggttgttt caacattgtc attgattttg
      181 attgggacat tttttatggt ctttatcaat ttaccgtttc ctggatggaa tgaatttatt
      241 gccccttatt cggcaacgat tgttttaccc tttaggatga cgatgggatt gatggctatt
      301 tatgcaactt ttgttatggg ttcggatcta gctaaatcat atggattaga tagtgtgaca
      361 ggtggtatat tatctttagg aacgttcttt atgttacaag ttcctgtaaa cgttttaact
      421 cctgaagagg cacctcttgg ctgggtctta cctatgagtt ctttgggagc atctgggatg
      481 tttgcgggga tcttatctat gatttttgca gtggaagtat atcgtttctt taaaaatcga
      541 aatattacta tcaaaatgcc agaacaagtg ccacctgctg ttgcccgtag ttttgaggca
      601 ttgattccag gtgcagtcat tttaacaatt acttggctga ttcgaagcgt cgcgggcttt
      661 gatgtaaacg ctgccttatt atcgttattc gagcctttaa ccaatatttt agggaataat
      721 ttgcttggtg ttttactacc aatgtttttg atccatttgt tgtggtcatt tggtatccat
      781 ggcatgagta ttgtaggggc tgttgttcgt ccaatgtggc tgatcatgtt ggatgagaat
      841 gcaaaggcat tggcagatgg tacagctgcc acccaattac cctatatcgc tcctgaacag
      901 ttttatcaat ggacggtaac aattggtggt gcaggagcaa cgatcgtggt atctgttttg
      961 tttatattct tctgtaaatc aaaattcttg aaagaggttg gtcgtttttc tattattcct
     1021 ggtatcttca atatcaatga gcccataatt tttggagctc ctatgatgtt gaatccttac
     1081 atgttcatcc cgttcaactt agttccttta gttttgacta ttatcagtta ttttgcagtc
     1141 aaattagaaa tggtcaatag atttactgtt ttgccaacat ggacattacc agctccaatc
     1201 ggcggttatc tttcagcagg aaatgactgg cgtgttgtta tcttgatcat gatcaacaca
     1261 ttgatagcat ttattatcta ttatccattt gtaaaagctt atgataaaaa gatgctgtca
     1321 gatgaacaaa gtcgtggaga aaacgaagtc cagactgtct aagtttggtt tttagaaggt
     1381 gggaaacaat gtattggctg attttatttt ttgtttttat ctttttatta acggctagtc
     1441 accttatatt gaatatgcta gctacatatc atattcaaat caatcgatgg atttgggcac
     1501 tggcatcatt tttgatcgtg attcttccaa agatcattgt accgcatatg aatgttttgt
     1561 tttcatgggg gacatatgta ctttgtggaa tttttgctat caattttatg atcgagcagc
     1621 atagatggtt tgtcacatcg aagctttaaa tgttctaaaa gtggttgtaa cagaagtggt
     1681 tagcttcaag aaataagaag aaatttacga aaattgcttc ttaaattttt gtgaaatgtt
     1741 ggcttatttt tcgtttggtt gaacttatta ccgcagatgc aagcttttga ccaccgttta
     1801 ttcggaatat gggactgtga caaggctttt gtcacagccc ttttttacat aaagattatt
     1861 attgttttaa ctgttttggc agaaagaagg aggaaaaaat ggaaatttta tcagcaatgg
     1921 atttagaatt agtagatcta ctaccaaagt gatcaagtca aatatagatt tgggagctta
     1981 caaccatata gtaggggctg cagtatggtg tatgaatggt aatatgtatg atgtgagtgt
     2041 ttattcaaat catgaaacgt gtatagaaat aattgtgctg ggaatagcaa tcagtcatgg
     2101 tgaacgtgac ttttagtgta tcgttgctga cgacatagaa cattctgaca tgatctactc
     2161 aatatgtggg gattgctggc agtttatata ggagtatgca tcggattgcg agatcattgt
     2221 ctcaacagaa gagggaggtt gattatcaaa gataaagtgg taaatcctac atttgattta
     2281 gtaattgttg aggggactag cggcacaaat ctctacaaag cgatgcttct agatctatat
     2341 gacacatttt aagaaaatgt atttgcctaa ttattatggt ctttcttttg aagaaacgtt
     2401 gagatgatcc aaccagagaa aggtgtcaaa gaatttgact aagaataaat ggcagacgct
     2461 acctatgctc atttggatag cgtcagatct tttattttaa ggatgatttt gtgtttttta
     2521 gacaccgtaa tttaagtaac tctaactttt caatccacta tttacttttt ttgtattttc
     2581 actgtctggg tctcgaatag cgaaattact gatgagtctc tattaaaaaa gctttttcta
     2641 cttgaaacta tattgggtgg ttgctcagga aggtttcgat ttcttgaatt gcttgttgtg
     2701 gaccatttat tggaaaaacg gcttgcagcg tgcgtgcctg ctgacgaatc ttgggtgaag
     2761 aaacttcgcg cagagcaatc tgcagttttc tttcagtcac tggttttgtg agggcgattg
     2821 cgtttccgta ttttacacaa tagtcaatat tacagctttg ctcaaattgc atccccacac
     2881 cgataaacgg tttgccggat tcacaggcag tttggacggt cccttccccg ccgtggataa
     2941 aagaaaaatc aatgaattca tgcaaaagat gactagacag atattctgtt attaaaagat
     3001 cttctgagtc agattgaatg tcatcaagca gatagtcaat aggtgcaata accgctagat
     3061 ccggttgttt ttgtaagaag aacaaaattt tgcgtaacat acgcggattg cctgaactac
     3121 ccattgcgaa atagataata cgttttcttt cttgttgctt tcgctgtata aaagttagca
     3181 cttcttcagg caaggaagta ctgagcttag caaaaattgg accaacgatt cgataatttt
     3241 ccggcagctg ttcttcttcc aaaaatagtt taggcgttgt gatgagattt agatcagcat
     3301 ccatcatttg taaacttgtt tttggtaaag gaagcatttc tttttgtgca gcgtgtttga
     3361 aggcattggg gatccatcgg gtatttacta tacgcttttt tgtccagtgc caaaggggat
     3421 tgcttaaagg gccgaagaaa cgtaactcgt ggggcatcag cgcatgctct gattgaaaaa
     3481 aaggttcact caaagcatag ggtttaacat atactaaagg aatcttttta ttgcgggccg
     3541 ataaaaaaat tgtcacattc gaacctgtca caatcagtcg aggctcaaac ttagcaatca
     3601 gactctgttc actagtgata cgagaggcaa ccatttcttt ggtaaaaggg tttttaagtg
     3661 tacgcaactg gtcaaatttt atgatttgtt cttcttgttt tttcgtaagg atcggcgcta
     3721 gtaaatgata ctcaaagcca ctttctgtga tcaaaggagc aaaagtttta gaaaagccaa
     3781 agaatacaca gtaataacgg tctttcatag ctttggcaac ctcaatcatt cgtgttgtct
     3841 cagctaaatt gaatactgca ggagcaaaaa gaattgtctt catgaaatcc tccttattgg
     3901 aatgagtcta tcccttcgtt tcttactagt atactaaaaa acacagcgtt cgagtagcct
     3961 atgtatattt tgaatattaa actgtctctc gctgttttca caacttctaa atttaaattt
     4021 tgtaatcaag ttgaaactcc aattttcaaa aaaacacgca acaatttttt gatcgattta
     4081 tatgttgtgc cagataaata cgagaagttg tgtcattaaa aataaacatg agaacataaa
     4141 aaaataaaat aattatatta attgataggt gtgctgtcgt cattatacac acgtaagaaa
     4201 gaaatagtta tgtttaaaaa tattcttaga ctttctcgcc atgacaattt tcactatgtg
     4261 ttataactgt aaacgataat tattcagata acacaaaaag aaatcaagg
//
LOCUS       DAHHZJ010000190         4206 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.193, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000190 DAHHZJ010000000
VERSION     DAHHZJ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.193"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            161..391
                     /locus_tag="NBL02_002604"
     CDS             161..391
                     /locus_tag="NBL02_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HCD2573239.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            488..1789
                     /gene="murA"
                     /locus_tag="NBL02_002605"
     CDS             488..1789
                     /gene="murA"
                     /locus_tag="NBL02_002605"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCD2573240.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1802..1978
                     /locus_tag="NBL02_002606"
     CDS             1802..1978
                     /locus_tag="NBL02_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCD2573241.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2113..2409
                     /gene="yidD"
                     /locus_tag="NBL02_002607"
     CDS             2113..2409
                     /gene="yidD"
                     /locus_tag="NBL02_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCD2573242.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2593..3060
                     /locus_tag="NBL02_002608"
     CDS             2593..3060
                     /locus_tag="NBL02_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD2573243.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3145..4029
                     /locus_tag="NBL02_002609"
     CDS             3145..4029
                     /locus_tag="NBL02_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCD2573244.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4143..>4206
                     /locus_tag="NBL02_002610"
     CDS             4143..>4206
                     /locus_tag="NBL02_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HCD2573245.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIK"
BASE COUNT         1415 a          650 c          919 g         1222 t
ORIGIN      
        1 tttgtctcag tccctttttt tgcatatttt tagcatttat ttcctaaaaa atgtaagggg
       61 attcctatat ttttaagtat acttgttttt ccttttatac attttatggt attatcattt
      121 tattagttat tataaaacaa tcgttagaaa ggacgtcatt atgcaagtat acggaattga
      181 tgcggtgctt cgaatcgtga gtcatttggc gtttatctac ttagccttct ggtctttacg
      241 ttcgttacgc attgaaactt ttttcagacc aatgcataat acgcaaatta gaatggcaat
      301 cgtacttttt tcaatttttt tagggtacac tgcaagtaat tttttcttag aagttatcgc
      361 tttatgccga aatcttttcg ttagcctgta aattttaaaa aaaggtacta ttttaaaagc
      421 aaggatgtta taatagaaaa gattttgagt aagtgtatgc tcattggaat tttttggagg
      481 gaacaacatg gaagagatca tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat
      541 tgaaggagct aaaaatgctg ttttgcctat tttagctgct agtttgttag cagaagaagg
      601 tattacgaca ttagacaacg ttccgattct ttctgatgtc ttcactatga atcaagtgat
      661 tcgtcactta aacgtagatg tagattttga tgaacaaaag aatcaagtaa caatcgatgc
      721 ttcaagacaa ttagaaatcg aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat
      781 cgttgtgatg ggtccgctac ttgccagaaa tggacatgca aaagtagcta tgcctggtgg
      841 atgtgcgatt ggtaaacgac caatcgattt gcatttaaaa ggatttcaag ctttaggagc
      901 aaaaattatt caaaaaaacg gctatatcga agcaattgcg gatgaattga tcggtaacac
      961 gatttattta gatttcccta gtgtaggtgc tactcaaaat atcatgatgg ctgccgtaaa
     1021 agcaaaagga acaaccatca ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc
     1081 aaacattttg aataaaatgg gcgctcaagt ctatggagct ggtactgaaa caatgcgtat
     1141 tgaaggcgtg gatcatttac atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc
     1201 aggtaccttt atggttgctg ctgcaatgac acaaggcaat gtgctgattg cagatgctat
     1261 ctctgaacat aaccgcccat tgatttctaa attgatcgaa atgggtgcag aaatcattga
     1321 agaagaaggc ggagtccgtg tcatcggacc aaaacatatc ttacctacag atgtaaaaac
     1381 gatgcctcat cctggtttcc ctacagatat gcaagctcaa atgacagcta tccaattagt
     1441 tgcagaaggt acaagtgtag tgactgaaac agtgtttgaa aatcgtttcc aacatttaga
     1501 agagatgcgc cgtatgaacg ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg
     1561 aaaccatgaa ttgcaaggtg cagaagtata tgcaacagac ttgcgggcag cagcagcatt
     1621 agttttagct ggcttgaaag caaatggaat cacacgtgtg cgtaacttga attatctaga
     1681 ccgtggatat tataatttcc atatcaaact acagcaatta ggtgcagacg ttgaacgtgt
     1741 agacatggat caaacttctg ctgaaaaaac agcacaaacg attgcttaag gaagtggctg
     1801 aatgagtaac caacgatata tcttattaac tttgataaag attttagtgg tgatcttatt
     1861 gcttatcctt ctttttgtgg ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa
     1921 tccatttaaa gtattccagc caagtttgtg gattcatatt cgagactttt ttcattgata
     1981 aataagaatc acagtggttc tagtcgatat gaagtgagcc atgaagcaga acatgacaaa
     2041 agaaaacaga agggaacgtt gacgttagtc gcgatccctt tttttagtaa aagaaaagaa
     2101 ggaatctaat tgatgaagaa gatattgatt tatttagtta gaggatatca gcgctttatt
     2161 tcgcctgcat ttccacctag ctgccgttat tacccaacct gttccaatta tatgataaaa
     2221 gcaattcaaa cccatggtgc actaaaaggg acggtaatgg ggactgcacg gatcttacgc
     2281 tgtcatcctt ttgtcaaagg cggtatcgat tatgtaccat tgacattcca attaacgcga
     2341 aataagaaag ataaaaaaga accagattat aaaaaggttg tgaaaaatac gagtagcact
     2401 gagcgataaa gaaacaattt ggaaaatagc ttcttatatt ttgcaaaatt gtgagttatt
     2461 gccgaagtgt tggattttga acaccgtcaa aaaaaggttg tgaaaaatac gggtagcact
     2521 gagcgataaa gaaaaagagg aaaattattg tcaaagtaac aactccgcaa ctacttatat
     2581 gggggacaaa aaatgatcga agaactaaac aagaaagatt taccatggtc cttgttatta
     2641 gatgcagatc ctgataaaga aaaagttcaa gtatatgttt ccagagggag tggctggata
     2701 tggaaagaaa aagaaaaaac aataggtgta ctgatctatg tggcaaggga aaaagaatac
     2761 gaaatcgtca atgtagcagt agccccaacg tatcaaggaa aggggattgg cggaaaatta
     2821 cttgagacag cttttcagaa gctttcacaa ctcgcttcca gtcaaactca gatcatcata
     2881 cgaaccggca gtactagcag tgcggcgtta catctctatc aaaaaatggg ctatgtggag
     2941 attggtcgag tcaaagacta ttttatccat cattactctg agcccatttt tgaaaatgga
     3001 gatcaattaa gggatcaagt aattttagca attcacttac ctaaaaatag ctttaactaa
     3061 taaactttca attcgagaaa atgctttcaa atgctaaata gatacattac aatagcttta
     3121 gcaaacaaaa aggagaatat ttttatggga atattagttt ttgattttgg tggatcggct
     3181 gtcaaatatg gttgttggga tggcaaagag attaaaggta agggacaatt tgcaacgcct
     3241 gaatcttggg aagaaatgaa agcgcaatta tttcaagttt ataaaaaaat actcgtttct
     3301 tttgaagtcg aaggaattgg aatcagctct cctggagtag tgaataatga gaaacaggtg
     3361 attgagggaa tcagtgcgat tccttatatt catggattta atattttccg agatttggaa
     3421 actctgtttc aactcccagt aacgattgaa aatgacgcca actgtgccgg aatggctgaa
     3481 ttttatgaag gtgctgcccg tgattatcaa gatgttgctt ttgttgtggc aggaactggg
     3541 attggcgggg cgttgttcac gaaaggacaa atcaataaag gggcacatct ttatggtgga
     3601 gaatttgggt tgatgttctt aaatggcgat aagacattca gtaaacttgg tacagcggta
     3661 cagatggctt ggcgttattg tgaacgcaaa ggtctcgata aaaattctta taccggaaaa
     3721 gatgtctttg aattagcaga aactggagat gcaatagcaa aagaagaagt tgagagtttt
     3781 tatacgtatc ttacaaaggg attattcagt attcaatttt cgtttgatcc agaagtgatc
     3841 gtattaggcg gcggtgtttc tgcaaaagaa gggctcattg atgagatcaa aagccgaatg
     3901 aagaagctga cagaacaatt cgatctgcat gatttcgaac cgcagatttt attatgtgaa
     3961 tacagaaacg atgctaatct tgtaggtgca gcagcaaact atctcgctgt acgtcaaggg
     4021 gagaattaag agctttagag aaggtgcagc ttgcataaaa gaagtgaatg aaatgaatag
     4081 gagattttta cctaaggaat gttttatttg ctgcagtatg ataaaatatc tgagaggtga
     4141 aaatgatgtc aaaaaaaaat aaagatgttg aagtgcgtat agaagaagtg aaaaaagata
     4201 tcaaag
//
LOCUS       DAHHZJ010000191         4162 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.194, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000191 DAHHZJ010000000
VERSION     DAHHZJ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.194"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(<1..225)
                     /locus_tag="NBL02_002611"
     CDS             complement(<1..225)
                     /locus_tag="NBL02_002611"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="malic enzyme; oxaloacetate-decarboxylating;
                     NAD-dependent; catalyzes the formation of pyruvate form
                     malate; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HCD2573246.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLF"
     gene            complement(666..1985)
                     /locus_tag="NBL02_002612"
     CDS             complement(666..1985)
                     /locus_tag="NBL02_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HCD2573247.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            2242..2712
                     /locus_tag="NBL02_002613"
     CDS             2242..2712
                     /locus_tag="NBL02_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HCD2573248.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            2934..4037
                     /locus_tag="NBL02_002614"
     CDS             2934..4037
                     /locus_tag="NBL02_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HCD2573249.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT         1347 a          693 c          877 g         1245 t
ORIGIN      
        1 aaacaatgtc cggttcgtat tgaatagatt cattaagaag atacgttttt ccaaatcggt
       61 ctgtttgctt aagtattgac cgtaagcttg gacagattgt tgttccaatg tttggacagt
      121 acttggaagc ataccagtga ttccgtattt tgcccgttct tcttttgtaa aagctgttcc
      181 tttatttaga aaaggattat ttaataagtt tagccctgta agcatgaatg attcctccta
      241 agttttaata caaacgaagt ataggagtca gatgaggata tagtcaaaaa gagcaggtat
      301 aataaatttt atttatatga gtaatagagt caaaagaaat caaaatagcg gtatttgttc
      361 tttttatatc aacaatcctt gcttgattac tatgcgtatc tcttatatgc taaagaaaga
      421 aaagtagaag aataaaggat atgttagata gataaataga atcctgaata attcatagct
      481 ttaattcttt ggcacatttt attgaaatat gtatcactaa tatccctatc aactgatttt
      541 gactaaaaag ttacttcaaa cattcactgt tcaaaaatat tgaggaataa attttgggtg
      601 taaggcattt tgagcatact tctccctgga taacttccca gtaaaaaaat cgttagattg
      661 ttggattaga tccattgact ggctcgcctc aggcgagcaa agaccctgta gaattcattc
      721 tgatacacat gataactaga taatttgaga tagactcgtc tacccgtttg gactaatttc
      781 ccagcaactt tcagaaactt caatcgaata gaatgaatgg tcattccctt ttggacttct
      841 tcaaagccaa tcgttcttaa gaagttgact aagttgtaag ctatcaagct gagcatcatt
      901 cggacatgat tctccaaaaa gcgaggactg tctgttttgt caaagtaaaa gccagctttc
      961 gcttctttaa tgaagttctc cattgtgcca cgtttggcat agatagaaaa gatggtatca
     1021 ggagaaacat tttctgatag attcgtcacg ataaattcat gtcgaaagag tagttcgccc
     1081 gcttcacgtg ttgagcgtat acacacgcga cgactttgtg accaggattg tgcttgataa
     1141 gtggtcgaga agtactgaac ttctcgttct tcccactttt gattatcgcc ataaagtact
     1201 gatttctcag ctatttgacc tagtctacga ttattcttca gtcgaataac ataatgactc
     1261 ttttttgatt cacacgaatc atacacacca ggtgtagcga acccgctgtc tccacgaacc
     1321 aagatgtcag tgtttggtag agagtgatta tagtgctcta ataaaggtgt gagaaactcc
     1381 tttacgcctt ttgatgtata ttgatttcct gaacgtagtt cagcttttaa gaagtcacca
     1441 gtcaatccgt caaaagctac caatggatgg taaccatagg tttggtagtg tgtattataa
     1501 ttcgtttgtt cttggtgacc aaatgtatct gaatgggtcg aatctaaatc aatgattaat
     1561 tccgtatcat tacggatgag gcgtgcttta tcgataagtt cttggttcaa agcttgaagt
     1621 tcatggatat tctcctcaga tagtcgatct aaaaatcggg aaagtgaaga ttgagaagcg
     1681 agttctttcc tatctaaaac agctttaaac acaggatctt gtctcaggag attagctgca
     1741 gaatcagctg agtaaccagc aattaattgc ataatgaact gctcaagtat ggataagttg
     1801 tcatgagtaa aatacgctcg ttcgtcttca atgtgaatgt gttgcttagc cagttcagaa
     1861 aaaccaaaga tgttcatcag ctctttcact aggacgagac ccgaatcact cgataattga
     1921 ccacctgtat gagaaatagt gatatttgaa ttgaatttta cttggttttt gtgtaagcta
     1981 gtcatgaaaa gaactccttt cttttggttg gtttagtcac tttaaccata gcagaaaggg
     2041 gttctttttt catcacttaa cgggtgaaga tgaaaaagta attgtaagct gccgtgaggc
     2101 agtttcacat ggttttaatt aaaagtatga attattcagg tagaatctct gaagaataaa
     2161 tcacgtgatc gtgacgataa aatacactta cgtcaggtga taaaaaatac ataagtagaa
     2221 aactaagagg aggaacagaa aatgaaagaa gttcaattga tcagaaaaag cgaactatca
     2281 gaaggtggtt gcaatgcatg cggcgtagta gaagcaacaa gctatacatt aaagcttggt
     2341 gccaacaaag cgataatctc tgagttgaca gtaggcggtc ttgtggacag tctggcgctt
     2401 gctgaaggtt ttattggaga agatatttat gagatgtttt cagaaatccg tcagttaaaa
     2461 aaaggggaaa actgcataga ggttcatcat gagagcccga atgtgagatt caaacgtggt
     2521 gataatgaga tgatcttcaa taatcatgtg tccgatcaca ccgagctata tggaatcgtg
     2581 aatcaaatac taactgagct ttttggattg ggaccttatg cgttcaagga agaaaacggt
     2641 aatccaaaat taaacgaaga atggcaagaa acgattgaaa tacaaagaaa caacccgcac
     2701 ttatttcaat aggcatccac agtatttcgt aaaagggact ttgacacgac ttttgtcaaa
     2761 atcccatcgt tttttatcta tttgtgttct aaacatttct ttcagagtag taacaactgg
     2821 aatgactgaa atgtaaacag attatttgcc tgtatcaata cggaagagta atataaagtg
     2881 tgtaaacgct atcatattat tcattcaatt ttttatagag ggaaggcatt gaaatggaaa
     2941 aaacgtatca agcaggtaca aatgaaggaa tagtggattt tatcaatatg gaagatttag
     3001 aaatagccgc ttcacaagtg attccagctg gtggttatgg ttacatcagc agtggtgctg
     3061 gcgacttatt tacttatcaa gaaaatgaac gggcattcaa tcatcagctg attattcctc
     3121 atgtattaag agatgtagaa ttacccgata caactacaca ttttgacaaa gaaacgctga
     3181 ctgcaccaat catcatggca ccagttgctg ctcatggctt agctcatgtg aaggcggaga
     3241 aagcttctgc aaaaggtgta gcagattttg ggacgatcta tacagcaagt tcttacgctt
     3301 cttgtacatt agaagagata agagaagcag gcggggagaa agcaccgcaa tggtttcaat
     3361 tttatatgag caaagatgat gggatcaatt tggatattct tgaagtggcc aagcgtaatg
     3421 gagcaaaagc aatcgtattg acagcggatg caacagttgg cggaaaccgt gaaacagacc
     3481 gcagaaatgg gtttaccttc ccactgccaa tgccgatcgt tcaggcttat caatcaggag
     3541 taggccaaac gatggatgct gtgtacaaat cttccaaaca gaagcttagt ccaaaagatg
     3601 tagaatttat tgctgctcat tctgatttgc cagtctatgt gaaaggtgtc cagtcagaag
     3661 aagatgtcta tcgttcatta gaatctggtg ctggcggaat ctgggtatct aatcatggtg
     3721 gccgtcaact agatggtggt ccagcagcgt ttgattcgtt gcaatatgtg gcagaagcag
     3781 tagataaacg tgtaccaatc gtgtttgaca gcggtgtacg acgcggacag catgttttca
     3841 aagcaatcgc atcaggtgca gatcttgttg ctattggcag acccgttatt tatggattgt
     3901 ctttaggagg aagtacagga gtccatcaag tgtttgattt ctttaaaaca gaattggaaa
     3961 tggtcatgca attagctgga acacaaacag tagaagatat caaaaaaata aaattgcgtg
     4021 agaatcgttt tatttaagaa acaaatgacg aaaaaaatag gagtgcggca gatgatgccg
     4081 tactcctatt tttatagttc aaggatgtca ctaatagaat aatttgaaac gaaaaaataa
     4141 aattgaaata ttttttcata ct
//
LOCUS       DAHHZJ010000192         4114 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.196, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000192 DAHHZJ010000000
VERSION     DAHHZJ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.196"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(224..1729)
                     /locus_tag="NBL02_002615"
     CDS             complement(224..1729)
                     /locus_tag="NBL02_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCD2573250.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDN
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1726..2412)
                     /locus_tag="NBL02_002616"
     CDS             complement(1726..2412)
                     /locus_tag="NBL02_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD2573251.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2605..4026)
                     /gene="gndA"
                     /locus_tag="NBL02_002617"
     CDS             complement(2605..4026)
                     /gene="gndA"
                     /locus_tag="NBL02_002617"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCD2573252.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1138 a          864 c          696 g         1416 t
ORIGIN      
        1 aatagaaaat tccctatttt tatttattat ttttaaacac gaatattaca gcattttatt
       61 gttttttcgt tcatctaaaa gatacttaca tttaattaac ggatgttatt ctatttaatt
      121 gttcgctagt ttttttataa tgacatccct gaatttctca cttgcttgta ttttgtacct
      181 tattctttgt tggatatata aaaaaacaag taaagcacat gctttacttg tcttctgcct
      241 ttgcgatagg aataaagatt ctaaaaattg tcccttgccc tactacactt tctgctaaga
      301 tacgtccttt atagttttca accagttgtt tagcgataga caaccccaga ccatttccac
      361 ccttcgttct agctcttgca ttatccactc gataaaaacg attgaagatc ttattaaggt
      421 cttctttggg tattccttcg ccaaagtcct gaatcgcgat ctcaaattca ttcagagtgg
      481 aagaaataga gatatggact tcttttcgtg tagttgaata ctttactgcg ttatccaata
      541 tgataatgat taattgttca aagtgattac gataaatttg cagggtcact tcttttgtca
      601 aatcatcatc taaagtgatc acatagtctg gatataatat cttgaaatta ttataaactt
      661 gataggtgac ttcttttcct ttagttgttt cattcgcata atgaacatct acttgttctg
      721 ctcttgataa gtctagcatc tcttgaacta agcttttcat acggctgatt tcttgcatgc
      781 tggcgcttaa tgattcttct aatatctcag gatcatcttt cccccaacga tttagcagac
      841 taagatgccc ttcaataatt gcaacaggtg tccttaactc atgagatacg tcttccacga
      901 attgctcttg ttgttcaata tacaaacgca tacggtcaag catctcattg aatatttcag
      961 ctaaatctgc caattcatca tttgtgtcga tttcaggcat gtggatatct gattgcggat
     1021 ctttacgtat agtatccatt gtatctctga gtaccttgag tggttttaag aaataagaag
     1081 ataaaaagaa acctaaaata ctacttaaga tcaaagaaac aacttctaaa acaataagag
     1141 tcaataatag cttatttcga atatcataga aagaagataa ttcatagaag ctttgcgcat
     1201 agccgatctt ttctcttgta cctttagaat atataggctg gatagataaa aagccagttt
     1261 ttccttctag tgtaacgatc gttggaactt tacgtgtagt ctgaatcagg tttcgactgt
     1321 tttcatgggt tttaaaaatc aagttctcgt ccaaatcata aacataaaga tccattgatg
     1381 gctgccctag ctcagctata aaggaatcga tcttcattaa gtttccttcg accagtgaag
     1441 tatgatctac gacctcatct ccgtcatttg tcgctttcgt cagattacga tacgtattta
     1501 ctaatgtcag ttcttcatcc gagttagcca aacgagaagc tacttctgaa atcgtacgct
     1561 ctacattgtt tctttctttt gccacaatca gattgataga tgatttatag gtaatgacag
     1621 cgaaaattgt aaacacaacg aatatgaaga aagaactcgc aaaggcccac ttgattgtca
     1681 aggagggccc tttcagttct cttttttccg attttttcgg ctttatcacg agcgcatcac
     1741 ataacctgtt ccgcgtacag tttggatata gctttcctcc ccgggaacgt caattttgtt
     1801 gcgaagataa cgaatatata cgtccactac gtttgtttca acttctgttt cgtatcccca
     1861 cactttattt agtaaaacgt cacgtgctaa taccacgttc acgttttcca ttaaagtaag
     1921 cagcaactcg tattctcttt ttgttagttc aattacttca tttccgcgac gtacgacacg
     1981 gttttctttt tcgatagtca aatcgcgata agtaatagta gtttgtttag ccacattttt
     2041 atcgccttca atatcgattc gacgcaacaa tgcacgaaga cgtgctaata attcttcgat
     2101 agcaaatggt ttgacgatgt agtcatctgc tccgtggtca agtccagata cacgatcgat
     2161 cacagaatct cttgcagtca tcataatgat tggtgtattt ttgacttggc gtacacgtct
     2221 gcagacttct aaaccgttca attctgggag catcagatcc aacaatatgg catcccactc
     2281 attatttaat gccgcatcta aaccagttcg gccgttgtag tggacttctg cgttataccc
     2341 ttcatgcttc aactctagct cgacaaatcg tgccaagttc ttttcatctt caatgattag
     2401 aatattgctc atttattatt ttatcctttc tattctactg gctttttaaa aactctatca
     2461 ttataccgat aatatgctta aaaatctata gtcattttat catggatagc aattttaaaa
     2521 aagagctgaa gtctactcat aacaatcgac ttcagctctt tcgtcctctt tttattttct
     2581 catttcctaa cagccgaaag actattattc ttcatggtac catgagtaat ggaagattcc
     2641 ttctttgtcg acacgctcat aggtatgtgc gccaaagtaa tcgcgttggg cttggattat
     2701 atttgcaggc agacgttctg ctctgtatga atcgaagtaa gcgatggctg atgagaaagt
     2761 cggtacaggt acgccagctt ggacagccaa ggcaacgacg tctctgactg attgctgata
     2821 ttttttcgtg atttcaacga agtaatcatc tagcaataaa ttttcgatct caggattttt
     2881 gtcataggca tccgtaattt tttgcaagaa tcgagctcgg atgatacatc cagcgcgcca
     2941 gatttttgcg atttcaccaa atggcaagtc ccaatcaaat tcttttgaag cgacacgtaa
     3001 ttgagcaaat ccttgggcat aactcatgat tttactgaaa tacaacgctt ctcggatttt
     3061 ttcgaccaat tcttttttgt ctcccgtgaa ctcaaaatcg ttcgtacgag acaaaatctt
     3121 gcttgcttgt acgcgttctt ctttataagc agaaatatag cgggcaaata cagattcagt
     3181 gatcaatgga agtggcacac ctaagtcaag cgcgctttgg cttgtccatt tacctgttcc
     3241 tttattccca gctgcatcaa ggatcacatc tactactggt ttacctgttc cttcatcgtc
     3301 tttacgtgtc agaatgtcag cagtgatttc tatcaaatag ctgtctaatt cgccttgatt
     3361 ccattcttta aagatatcag ccatttcttc tactgaaagg tttaaaatat tcttcattaa
     3421 gtcataagat tcagcaatca gttgcatatc tccatactca ataccgttgt gtaccatttt
     3481 cacataatgt ccggctccat ttgggccaat atatgtgaca catggtgctc cgtcttctgc
     3541 ttttgcagaa atttgctcta agattggtgc aactagcgca taggcttctt tttgtccacc
     3601 aggcatgatt gaaggtcctt ttaaagctcc ttcttcacca ccggaaacgc ctgtaccgat
     3661 aaagttgatg ccagagttgg ctaactcttc attacgacgc atcgtatctt taaagaatgt
     3721 atttcctccg tcaatcaaaa tatcgccttt atcaaggtga ggaagtaact cttggattgt
     3781 tgcatctgtt gccggtcctg ctttgaccat tagcaagatc cgacgtggtt tctcgataga
     3841 ttctacaaat tcttcgattg tatatgttgc tttgaaattt ttatctgggt gttcttctac
     3901 tacagcggtt gttttagccc ctgtacgatt gaataaagcc actgaatagc cgcggctttc
     3961 tatgttcaag gctagatttt tacccataac ggccataccg acaacgccga tttgttgttt
     4021 tgacatatga actcctccta catacgggat ttctccctaa tctcttacat tatagcttaa
     4081 cacggctaca ttgcaaacta aagaacaaca tatt
//
LOCUS       DAHHZJ010000193         3770 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.197, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000193 DAHHZJ010000000
VERSION     DAHHZJ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.197"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(83..823)
                     /gene="truA"
                     /locus_tag="NBL02_002618"
     CDS             complement(83..823)
                     /gene="truA"
                     /locus_tag="NBL02_002618"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCD2573253.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(1014..1649)
                     /locus_tag="NBL02_002619"
     CDS             complement(1014..1649)
                     /locus_tag="NBL02_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HCD2573254.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(1796..3037)
                     /gene="sstT"
                     /locus_tag="NBL02_002620"
     CDS             complement(1796..3037)
                     /gene="sstT"
                     /locus_tag="NBL02_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCD2573255.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            3235..>3770
                     /locus_tag="NBL02_002621"
     CDS             3235..>3770
                     /locus_tag="NBL02_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573256.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTL"
BASE COUNT         1243 a          780 c          579 g         1168 t
ORIGIN      
        1 atttctttaa tttgcttcat acattattta acatcgatat ttatactgtg aaacatgcgt
       61 ttcatccatt atcaaagtat ttttatcgat aataaacttc atccaaaaac agaccttgtg
      121 cagggacggt ttctcctgca tgttgccgca ctttgctttc aaatatttca tcgattgttt
      181 ctatcggcat ggcacctgtg ccgatttcta gtaatgtccc catgataatc cgcaccattt
      241 tatataagaa tccgtctccg acaaaagtaa aatgaagcat ttctcccttt cgttcgatag
      301 ataattcatc aatcgtacgc actgtggatt tttttgattt tttcaatgca gaaaaaccaa
      361 tgaaatcatg tgtacccact aatttttcgc aagctgcatt cagcttgtcc atatccaact
      421 cttgagggaa ataaaagctg tggtttcgct caaatgctga aggaatcacg ttgttccaaa
      481 cataatagct gtattttttg cccaccgcat tatatcttga atggaagcgc tcaggcattt
      541 cttcgattct tttgatgaca atatcccgcg gaaggtaacg attcataaag tcgatcattt
      601 ctgccagtcc aatttctgtg tttgttttaa aatttgctac ttgtcctcgc gcatgggtgc
      661 cagcatctgt tcttcctgat ccaataattt ctatctcttc atttgtcatc tgatgcaata
      721 ctttttcgat ttttccttga atggttttat cgtcatctcc aagtctttgc catcctgaat
      781 atctttttcc gtcatattcg attgtcaact tgatgtttct catattttag tggctcctct
      841 gtcttccatt tggtcttctc tctacctttt taagattgta gcacaaaata tagctccttg
      901 cttctctaag acagaagcta tacaagaaaa ctgtggaaat atatatttca gaaaaacaaa
      961 aaacagccac cggatgaagt atccgctgac tgttcaattt gacggctcaa gttttactgt
     1021 ttgagatgca agatactcaa ccaggcaatg ggcacataat taaagaaaaa actgattctt
     1081 tggcaaaaac ctattttatt cagtaatggc caagaattta tagaaggaat tcttgccagt
     1141 ccataaatcg ccgcaaaaag caaactgatc acagacagtc caaggtataa gtggcttacg
     1201 tccatttgcc cattttttcg atagatcaag aaaagaagaa ctggaaacaa taaaaaacca
     1261 ctgtaaccta aacctgatcc gatattatgg acccatgtcg agacattcca cgaagattca
     1321 tgggtattga tactgaacaa acctgtaaag atacaatcac ctataccata gaacgcgatg
     1381 gctgctgtaa ctagggtagc taacacacga gatgtactaa caaatgcagc atacaatgca
     1441 ggcaatgaca ggatgaaaaa gatccctgaa accacagacc aaataagaaa cgcttttctt
     1501 acaggactgg aaacctctcc aaatacactg atcacgctct tcatttgatc taattcagga
     1561 taatacaatc ctaatacgta cggagtcaaa aaatcactca gcacgcctaa taataaaaag
     1621 aaaaaaccaa atttacgtaa aaaatccatg atacgcctcc aataacaatt acctatgctt
     1681 ttaattgtta ttataaacga ctttatcttg atttaacaga aacctctatt tacacgaaca
     1741 tctatataac tcattaaaaa ctcgaatcat tttaaaaata ctaaatatct aacgttcaac
     1801 gttcacttaa tccatctaaa caatctttca atttgatttt ttccccactt cttttacgcg
     1861 cgcctagctc tcctattgca gtaaacaaca gatcacttga cgaatttaag gcagtttcca
     1921 ttgaatcctg gataacaccg ataataaatc caataccgac gatttgcatc gcgacatcat
     1981 tagagatacc aaacaaacta catgctaatg gaatcaataa cagtgaaccg cccgcaacac
     2041 ctgaagctcc acaagcagct aaagcagaca aaatacataa aaggactttc aaaacaaaag
     2101 gtgcttcaat ccctaaagaa tgtacagcag tcaacgtcat caaggaaatg gtaatcgcgg
     2161 caccgcccat attgatcgta gcacctaacg ggatcgatat ggaataagac tcttttgtca
     2221 atttcaatct ttcagccaac atcatgttta tcgggatatt tgcggcagaa cttcgggtaa
     2281 agaaagcggg aagtgcgctt tctttcaaac aaaagaaaat caatggatag ggattttctt
     2341 taataaacaa aaacgtgagg aatgggtaca caactaatgc gacaaacagc atcgtaccga
     2401 tcaaaacaag taaaagctgc agatactctg ccaatccagc gattccagtc gtagcaattg
     2461 attgataaac caatcctaaa atccctatcg gagcaaatgc aataatcact tgtacgactt
     2521 gtgacaacgc tatagatatc tgatccacca actcttttgt cgattctgaa cgtgctcgaa
     2581 aagctagtcc caacaataca gcccagaata agacgccaag gtaattcgca tcaataagag
     2641 attgaacagg attttgtgtg acattcgtca acatagtaga taaaacactt tccaaacttt
     2701 gcggtgcttt ttcagatact gtttcttgta aagtaatagg aacagtaaaa attgaactta
     2761 caataactgc tacaagtgca gaaagcaatg ttcccactaa atataagatc aaaataggct
     2821 tcacgaatgt cttattccca attttatgct tggcaattga cgccatcgtc aagaaaaaaa
     2881 ccagaatagg tgcgattgcc ttcaagcaac tgacaaacaa cgttcccaat attgaaataa
     2941 atgtccatgt ggggaaaaag ataccaaaca atattccgac taaaatacca acaacaatac
     3001 gttgaactaa cgacatatcc aaaaacttct ctaacaaatg accacccttt cacaatttta
     3061 acaatcattg tatcatttta tgcaaagaaa ctaaaggtat aattttctaa aaattctaac
     3121 aatacaaaca accatttctt gctaattttg tactttttct gtctacacgt tgttttttga
     3181 gatattctaa gttaggatgt tattaatggg caacttatca ggaaacgttt tcttatgttt
     3241 ttgtccaatt tttttgtgag gtgtttattc ttgaaaaaaa taaagaagga gagttatggc
     3301 ttcctgacaa tctttgtcgc ttgtataatg atggttttac agcgtatatt gatttcagca
     3361 gtcactattc ctacatatgc aacaaaccct ggtcgattct acatctatac atttttacaa
     3421 acttccgttg ttattggtgc aaatttgatc ccactttaca tgggttacca atatcaaaaa
     3481 atcgcccgtt taaaagtttt acattattta agtcgttttg cctttatatt cctaatagcc
     3541 acactagttt gcaatatctt tttttacgta caagctggca gcttgaatat tcgtgattat
     3601 tggcttatcc taacacctat cagtcagaat tactttactt atgctgtttc ttgtactttg
     3661 cttttttgta gtattcctta tacagttgga tttttaaata aattatcgaa aacaacaatt
     3721 aaatatctac tattattttt aacaattgga ttagttggct gctctactct
//
LOCUS       DAHHZJ010000194         3692 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.198, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000194 DAHHZJ010000000
VERSION     DAHHZJ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.198"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(1..312)
                     /locus_tag="NBL02_002622"
                     /pseudo
     CDS             complement(1..312)
                     /locus_tag="NBL02_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(572..1120)
                     /locus_tag="NBL02_002623"
     CDS             complement(572..1120)
                     /locus_tag="NBL02_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573257.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1121..1978)
                     /locus_tag="NBL02_002624"
     CDS             complement(1121..1978)
                     /locus_tag="NBL02_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCD2573258.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2262..2549)
                     /locus_tag="NBL02_002625"
     CDS             complement(2262..2549)
                     /locus_tag="NBL02_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCD2573259.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(2539..2868)
                     /locus_tag="NBL02_002626"
     CDS             complement(2539..2868)
                     /locus_tag="NBL02_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCD2573260.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2945..3217)
                     /locus_tag="NBL02_002627"
     CDS             complement(2945..3217)
                     /locus_tag="NBL02_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCD2573261.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3217..3480)
                     /locus_tag="NBL02_002628"
     CDS             complement(3217..3480)
                     /locus_tag="NBL02_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCD2573262.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
BASE COUNT         1084 a          748 c          476 g         1384 t
ORIGIN      
        1 ctttgcaaca gaacccttga tctcgcttat tacaatcgct ttcatttgtc ctaagagtca
       61 aatctttttc caccataact tttacaccca ataaattttg cggtttattt ttctctgttg
      121 aaattctttg tgtagccttt aaactgacaa ttagctcatt gggtacatac ttatttgaga
      181 gtcatctaat tggctcataa tcctttcttt ttggttagta tctgcagtta cttgtttagt
      241 atgagccatg aacccaaaca tactcacccc aacaaccaat cctaaatata ttttcttttt
      301 aaactttttc acctacatct cctcctaata gttttatttt agagttagct ctattactta
      361 tttactaact caacaacaga ttatcataca cttgttaatg aaatcacaat atctcattaa
      421 aacacaccct aaaatcataa ccgttcctat gctcattttt tggatttttt aatcacgtaa
      481 ccagaaaaat aaatcccctc tatttcatgc aaatcaaaga agaactaagt aactaccgtt
      541 taaaggtagt tacttagttc ttctttgatt attatctaac tttattattc attccccacc
      601 ctttgcgcag tgagtcttcg gtatcttgtt ttatctgact ggtagttctt attatttctc
      661 cacgatcatc taattctgga acaattggat atttttctct gaacaagtct agttctttca
      721 ccacgtcatt aaacaaatct tgcttatctt cttgcgtaag attctgttta atatactcat
      781 ctaaagcttt tctaagcaat tcatctcttg tcatattagt ctcatacttt tctactaaaa
      841 ctctggaagt cgcatttaac aaagtcgaca gttttaaagg atgcttggtg gaataaacct
      901 tatcaaactt tctgattggt gcgccaactt tttttgttct tcctctactc tgtctttcaa
      961 cattggagga agtttttatt tcttgaactt ggtgcacttc taattgctta ttttcagatg
     1021 gtgtaattgc ttgctgcttt ttctttgctt ctgcttcttt ttttattttg gcgagtcctt
     1081 tgttgttccc aaaaatatca agtcctttga tgtcagccaa ttactccacc tccgcatacc
     1141 aattctcgtg ttcttccaat tctttcaaaa tactgataaa taattcgtgg cttttgcgat
     1201 cccattgttt taagagacct ttctttgtct tctccatcgt aatacctgat ccgtcgtatc
     1261 gttttaagcg cgcatttttg agtacaacat ttttaatcaa atgttcccca taaatctctt
     1321 gggcttgttt atacatttcc tgatctactg cgtcaccgct ttccaacatg aaaggaataa
     1381 caccagcaac atataaattt gtatcaaatt ctttcacaaa aaagtccata tactcaagat
     1441 attgcatcgc atttctcatc gattttactt gggtctgtaa gacgacgatt gcccaatccg
     1501 caatgtacat cgcggattta ctatatacag aaatagaagg tgggacatct aaatagatga
     1561 cgtcaaactc atcaaataat ggttctagct tttctttcat atatttgaat tgggataatg
     1621 aatcattata ttggtctttg atccaatcat catacatttc aaaggacatg tctgctggaa
     1681 gtatatagag gttattgatt actttataga cagcctcttt aggtggaaga ttgtttatca
     1741 acacgtcagt cacagtattt tcgtaaacaa tcggttcacc ttctccgcct ccttcttcgt
     1801 tagtgaagtt catatgcgtt aagttcatca catcagaagt atttccttgc atatccaagt
     1861 caattatcaa ggttcttttt ccttcattaa ctgctgattc aaaccctaac atttgcgcgc
     1921 acgtactttt acctacgcca cctttaaaat taccgattac atatttcttc gctaccacgt
     1981 tcctacctct tttcttttag caatattcat ttgatactat tataacatat ttatttttag
     2041 ttactaccaa aaaaagatat atatgtacta ccttataatt aataagtaaa aggtagtaca
     2101 taaataagga aaagctgaaa tatattgatt tatagctttc ctcaaagtaa tgacgttatt
     2161 taatttatcg agaagcagag aatactcgcg actttagtca tgtagacgaa tcagattttc
     2221 gataaaaaga agccgtcctt cttaagaacg acaacaaatc actagattaa ttttttcaac
     2281 tgttcataaa agttttcatg cgttccacat aacaatactg gaataacctt gttttctata
     2341 atctcgtaag ctactctata tgttgtgccc gcatatttga acccactaga ccaaatacct
     2401 gataaatccc cagttttttg ttcaccctta tatggatcaa tcgcaatttc atcataaatt
     2461 aaattcaaaa atttttcttt caatggcttc tcttttagct tttttaaata cttaatgaca
     2521 tgttttgaag gaacgatttc ataagccaat ttctgttgcc aactcctctt tagtcattac
     2581 tttagaatta gtaagtgtat cttcagcaat atctctaaag gaagaatgca ttttgttttt
     2641 tctattctta aattctacaa gaatctcatc tttatcgtag ccttctgcaa ttatatcaga
     2701 caaaatatct tcgctaaaat cataaaattc tttttgtggt tcaacaaatt cataaagaat
     2761 accattctct accaactttg cttcaacttc gactccatta ggaacattaa aatctttagg
     2821 tactgtcaac atgattgaat tgccttgttt tcttgttttt gtaatcattt aaatccctca
     2881 tttctaacag tatgtaatta cttagtaaat acattatacc acgagataat gtatttacaa
     2941 gtaattactg tgactcgtaa tgcgtccttg ctgaataaat ttcaaccacg tgttcatctt
     3001 catctacctt ataaacaacc cggtgttgtc tattcaatct tctagaatac ctaccttcat
     3061 gtgtaggttt taacttctca aatgattggg tagggagata gggatcttcc tttaaagtct
     3121 gaataacctt ctcaaattga ggtttcaaat tagactgttt gattttcttt aaatcagctt
     3181 tagccgagtt tttaatcatc acagaatagt tattcattat aagttatccc aatcaatttc
     3241 atcaacatta gtaaatccac tatcatcttt ttctcgctta cgaacaacac ccaaagtgcc
     3301 tatttgctca agtaatagcg tttctttgat tgctttccaa tcatccaaac caataagcac
     3361 cgcattattt tcactgcgct cactaatgat ttcaatttcc gtatgattct catttacatc
     3421 tttcaacaac ttataaaaat ttttccgtgc tgagctagga tttaagactg tttttgccat
     3481 atctactccc cctctaattt atatctccat tctaacgtac ttaattaagt acgtaaattc
     3541 gattgttttg tttaaattgg agtaccaaaa cagcattgta aagtgttatc cgtttccttt
     3601 atacccgtgg tgctagttaa ttttttccta gaggaaaatg tctaaacatg ttggtaaatc
     3661 aacaaaaaaa cgcatagagt agtctcaaaa ct
//
LOCUS       DAHHZJ010000195         3649 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.199, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000195 DAHHZJ010000000
VERSION     DAHHZJ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.199"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..104
                     /locus_tag="NBL02_002629"
     CDS             <1..104
                     /locus_tag="NBL02_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lincosamide nucleotidyltransferase Lnu(B)"
                     /protein_id="HCD2573263.1"
                     /translation="LLLVMDLQSHLIEQYNLKVTHDILERLLNYISE"
     gene            792..1613
                     /locus_tag="NBL02_002630"
                     /pseudo
     CDS             792..1613
                     /locus_tag="NBL02_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase zinc beta ribbon domain-containing
                     protein"
     gene            1756..1980
                     /locus_tag="NBL02_002631"
     CDS             1756..1980
                     /locus_tag="NBL02_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD2573264.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGDEIKISNE"
     gene            1995..2864
                     /locus_tag="NBL02_002632"
     CDS             1995..2864
                     /locus_tag="NBL02_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HCD2573265.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            2845..3579
                     /locus_tag="NBL02_002633"
     CDS             2845..3579
                     /locus_tag="NBL02_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCD2573266.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
BASE COUNT         1232 a          553 c          796 g         1068 t
ORIGIN      
        1 ctttgctatt agttatggat ttgcaaagtc accttattga acaatacaac ttaaaagtta
       61 cacatgacat tttagaaaga ttgttgaatt acattagtga ataggagagt aacgttattc
      121 acagtattgg gtactgaaaa ttaacagcat acctcagttt ttcaaaaaac agcgaagcct
      181 atggatatgt tcccgtctac cgatagtgca aaaagtgtcg tggatatgtt tccgagaacg
      241 gacatacaca aatgacattc attctgtcct ctcaatccat gtggaagcag tatgtctgtt
      301 aacaagaaaa taattattgt tcgtcctgac gagttgttag actcgaaaag gataaacatc
      361 atttttagaa ccggtagccg atttttggct agcggttttt tgggctcttt ggcaaatagt
      421 ggtgattgaa tctcgcgcta tcctatggaa aaccgccttg tgtgaggggc tgcgagaccg
      481 cttcgatcct agaattgctg aagcacgaat ctagcgtcac tcacatttca tcttttgacg
      541 tagctgagac aaggtgtttt tcttcgatcc gctagtggtt aatctctttt tggtgcaaat
      601 atggtttaga atttctttgc atggcttaag ggagtggaga atcggatatt agtaggattt
      661 acatccacag ataaaaatag gcttgatttt aacagcgaaa ctaaaatacc ctacttaaat
      721 gaagtgatag attagatgtt gggttgttag gagatgctta aaaatcctat gcaatcaata
      781 gtttttagtg tttgttgact tgatccctaa atcgcctgag tactaaacgt tgagtgtgtc
      841 tgcttactgt cactagccag taagtgagac tgtagttaat ctctcatttt ccccagtatt
      901 atgttgaaaa tagccatgaa gcgattattt ctaaagagtt atttttgcag gctcaagaag
      961 aaatttatcg gagaagtaat atctacacag gagaaggcaa gaacaaaaga atttatagta
     1021 gcaagtacgc tttaagtgcc atcaccttct gtggatattg tggcgatatt tataggagaa
     1081 cctattggaa tattcatggc agaaaagaat ttgtctggcg atgcgtgact agaatcgagc
     1141 agggtcccga ggtatgtaag aaccgaaccg taaaagaaga tgaactctat ggtgcggtaa
     1201 tgaccgcaat taataagcta cttgctggtg gcaacaatat gataaagaca ctggaagaaa
     1261 atattcatgc tgtgattgga gaaaccacag aataccaaat ttcagagatt aataccctac
     1321 tagatgaaaa acaaaaagaa ctaatcaagc tggccaataa aggtcaagat tatgaatacc
     1381 tggtagatga gatcgatgag atgcgagata agcgtcagac tcttttagta gaagatgcct
     1441 ccctcagtgg cgagaacgag cgcatcaatg agttgattga atttatccgc aagaacaaat
     1501 tccgcacctt agaatatgat gataagcttg taaggaagat aatccagaac gttaaggtct
     1561 atgaagacca cttcgtcata gccttcaaac caggtatcga aatggaaata tgaaaaccag
     1621 aagtaaatag cccatgactc tgaagtagag ttgtgggttt tcttttaatt gctgattata
     1681 ataatatttt aatgactatt gcatttatca accaaatggt ttatgttatt cttagcataa
     1741 acttggaggt gctgtatgac tacggcagaa atgattaaag aactgtgtga gcaaatgaat
     1801 ataagtgttt ccgaacttgc tagacgtatt ggccagactc cacagaattt taataaaaaa
     1861 ttacaacgag aaacagtaac cttggatgag ttgaaggcca ttgctgatgt gctaggtgtc
     1921 aagtttgtgc aggcatttat tttaccagat ggagatgaaa taaaaatatc taacgaataa
     1981 aggaggatgt ttatatggta ggtaatgtta ttcaaatagt aacagagaaa ttatcatctt
     2041 tgccttttat agaaggcatt gtattagggg gctcacgtgc aagaagcacc catacagaaa
     2101 attctgatat agatatcgga atctattaca attcagactc atttgacctg acagcgatta
     2161 atcaaattgc tacagagttg gatgatgaga atagaaacaa ccttgttgta cctcccggag
     2221 cgtggggtga ttgggttaat ggcggtggat ggttagttat aaacgggtgt catgttgact
     2281 tgatattacg tgatataaaa cgggtggaac aaataatcaa agatacggag caaggaattg
     2341 ttactgccaa ttatcagacc gggcatcccc atggttatat aagtgctatg tatcgtggag
     2401 aattagcgat tagtaaaata ctatatgcta agaacgaaag cttatgcgaa ttaaaaaagc
     2461 aggcagaaat ttaccctact gctctaaaga aaagcttgat gaactttttt atatttgaag
     2521 ctgaattctc tttaatgttt gtaaaagcaa atgcgggggc agaggataaa tattatattg
     2581 caggccatgt ttttcgtatt atttcatgct taaatcaagt actatttgca tgtaataatg
     2641 cttattgcat taacgaaaag aaagctataa aactgcttga aacttttgaa tataaacctg
     2701 aaaaatatgc cgaaagagtt aatcatattt ttgaagtact cggtctttct ctttttgaat
     2761 gctacgacat gaccgagaaa ctttataaag aagtgaatga aattgtatcg gagataaata
     2821 actttttaaa cgaggagagt tcagatgaaa gaaaacaaat atgatgataa tatatttttt
     2881 caaaaataca gtcaaatgag tcgctcgcag aaaggactgg ctggtgcggg agaatgggag
     2941 actttgaaaa agatgctacc tgattttaag ggtaagcgtg tgcttgattt aggatgcggc
     3001 tatggatggc actgtatata tgcgatggaa aacggtgctt cctctgtagt aggtgttgat
     3061 atttctcata aaatgctcga agtagcaaaa ggaaaaaccc attttccaca gattgaatat
     3121 gaatgctgtg ccatagaaga tgtggatttc ccagaggaga gctttgatgt aatactaagt
     3181 tcgcttgcgt ttcattatgt agcagactat gagaatttaa taaaaaagat atataggatg
     3241 ctgaaggctg gtggcaattt agtttttaca gttgaacatc ctgtttttac tgctcatgga
     3301 acacaagact ggtattataa cgaaaaagga gaaatactgc atttcccggt ggacaattat
     3361 tattatgagg gcaaacggac agctatgttt ttggaagaaa aggttacaaa atatcataga
     3421 acactgacca catatctaaa tacactgctt tcaaatagtt ttataataaa tcagattgtg
     3481 gagccacagc cgccagagaa catgatggat attccgggga tggcggatga aatgcgacgc
     3541 ccaatgatgc tgattgtatc ggcaaaaaag aagatgtaat aatatagaaa aaataaacga
     3601 ggagtatgta aatgagatca gaaaaagaaa tgatggattt agtactttc
//
LOCUS       DAHHZJ010000196         3639 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.200, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000196 DAHHZJ010000000
VERSION     DAHHZJ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.200"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..671
                     /locus_tag="NBL02_002634"
     CDS             <1..671
                     /locus_tag="NBL02_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCD2573267.1"
                     /translation="FILLNLLISVVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIIS
                     YFVYAFLSPDKGIFNQWITGHGGEAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFAS
                     VMGIDPTYYEAAMVDGASKWQQIKHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIP
                     RNSGALYDVTQVLDTYIYNGLTSTGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSD
                     SALF"
     gene            683..1603
                     /locus_tag="NBL02_002635"
     CDS             683..1603
                     /locus_tag="NBL02_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCD2573268.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1644..3101
                     /locus_tag="NBL02_002636"
     CDS             1644..3101
                     /locus_tag="NBL02_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCD2573269.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3446..>3639
                     /locus_tag="NBL02_002637"
     CDS             3446..>3639
                     /locus_tag="NBL02_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCD2573270.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWN"
BASE COUNT         1167 a          630 c          742 g         1100 t
ORIGIN      
        1 catttatcct tttgaatctg ctgatctcag tcgtatttgc aatcattatg agcgagttga
       61 gaaataaacg actcgttaaa gtttatcaaa cgatgtcctt actaccttat tttctatctt
      121 gggtcattat cagttacttt gtttacgctt ttttaagtcc tgacaaagga atattcaatc
      181 agtggatcac tggacatgga ggagaagctg tcaattggta taatgaaccg aaatattggc
      241 cgtttatctt ggtgttcatt ggtacatgga aagggattgg ttataacagc attatttact
      301 ttgcatctgt tatgggaatc gatccgactt attatgaagc tgctatggtt gatggagcta
      361 gcaaatggca gcagatcaaa catgtgacga taccacaact tattccacta atgacgattt
      421 taacgatttt agccgtagga aacattttcc gagctgattt tggcctgttt tataacattc
      481 ctaggaattc cggtgcatta tatgatgtga ctcaagtttt ggatacgtat atctacaatg
      541 gcctgacatc cacaggagat tttggtatga cggctgctgc aggactttac caatcagttg
      601 tcggctttgt cttgttgatg atcacaaata cgatcgcccg acgttttgat agcgattctg
      661 cattattcta aggaggaaaa aaatgaaaaa aaagaaagtg gagagagtcc aagtccgctc
      721 tttcaataag acggctaatt ttttctctaa tataatgatt gcactctttg ctatttcctg
      781 tattttgcca tttatatttg ttatcgccat ctcttttacc gatgagtcca tattgtcgac
      841 tcacgggtac agtttttggc cagaaaagtt cagtatattt ggttatacgt acttgttcga
      901 ccagatgcag gataaaattt tccaagcgct tttcgtcact gtacttgtta ctgttttagg
      961 tacactgatc aatagcacgg ctacttcttt atatgcttat gcgatttcac ggtcaaactt
     1021 tccattccgt cgttttttta ccgttttttg tttgattacg atgttgttct caccagggat
     1081 ggtggccaat tatcttgtta tgacaaatct tcttcagtta aaagatacga tttgggcatt
     1141 gatcttgcca atggctgtca gtcccttcaa tattattgtg atgcgcacat tctttaaacg
     1201 ttcggtctct gattcgatta ttgaatcggc acggatcgat ggagctagcg aactgcgaat
     1261 atttgttcag atcgttttac cgttagctgt tccaggtatt gcgacaatca gtttatttgc
     1321 cgcattgggt tactggaatg actggttcaa tgctttacta tacatccaag atgataagct
     1381 agttccacta cagtatcttt tgatgaaaat ccaaagtaat attcaatatc tgacacaaaa
     1441 ttcaggagca ggaagccaat tgacaggcgg attagcctca gttccaggag aatctgccag
     1501 aatggcgatc gtagtcatct cgacgttgcc tattgcgatc agctatccat ttttccagaa
     1561 gcattttgtc aaaggtctaa caatcggcgg agtaaaagaa taaagaaaca aaaatataaa
     1621 tcataaaatt aggaggaaga aaaatgaagt tatggaagaa actagccttt acaggtgttt
     1681 cagcgttgtt gttgggaaca ttagcagcat gcggaggttc tggttctaaa gaccaggcag
     1741 aagctagcaa ttcggacact ttgcagatgt atcaaatcgg agataaacca gataatttcg
     1801 atcaattgat ggaagtagca aacaaacgaa tcgaaaaaga gatcggtgta aaagtcaata
     1861 tcaattacat tggctggggc gattacgaaa agaaaatgaa cgtcatcatt tcttccgggg
     1921 aaaattatga tatcgccttt gcgaacaact atgtttccaa tgcccaaaaa ggagcctttg
     1981 ctgatttgac tgaattagcg cctaagtacg ctgaaaaagc ttataatgat ttagatgaag
     2041 catatattaa aggaaatctg gtcaatggaa aattatatgc tttcccagtc aatggaaatg
     2101 tctttgctca acaagtactt acttttaaca aagctttact agataaatac gatctttcaa
     2161 ttgatggaat ccaatcttac gctgatgcag aaaaagtctt gaaggaattc catgaaaaag
     2221 aaccaaacac ggcagctttt gccattggac aaggcttcaa agtccaaggg aactttgatt
     2281 atcctttagg gaatacgtta ccatttgcta ttgatctgga tggagatgat tcgaaaatca
     2341 tcaaccaata cgataatgaa acgtatatcg gactgttacg tacaatgcat agctggtaca
     2401 aacaaggact cattccaagt gacgctgcaa cgagcaataa ggattatcca ctagaaggaa
     2461 atacttggct aatgcgtgga gaaacacaag gaccgtatga ttatggcgat acaattttga
     2521 caaatgcagc aaaacaagaa cttgtctcaa aagcaattac tgttccacta aaatctacag
     2581 ctcaagcaca aatggcaaat tttgtcgtat caaatacttc aaaaaataaa gaaaaatcag
     2641 tagaacttct agggctgctt aacagtgatc cagaattatt gaatgggctt gtttggggga
     2701 tcgagggaga agcatgggaa aaagtcgatg gttcagatga taaaatcaaa ttgctagatg
     2761 gttatcagcc aaatacacat atgtctgctt ggaatacagg aaacaacaag attctatata
     2821 cacaagagtc tattacggat gacatgattg ccaaacgtga tcaatcgatt gctgatgcag
     2881 aaacttcacc gattttagga ttcagcttca acactgacag tgtcaaaaca gaattaagta
     2941 atatttctaa tgtcatgaat caatatttag atggattgaa tacagggact gttgatccag
     3001 atgaaacatt accgaaattg aaagacgctt tggacaaggc tggctatgat aaggtattga
     3061 aagaaatgca aaaacaatat gatgagttcc gacaagaata ataatgaata aagacaaagg
     3121 ggttgtgaca aaagacgtgt cacaacctct tgttccgaat aaacggtgtt caaaagccag
     3181 tttctcggta ttaagctaaa tatatgtaaa aatgtggaaa gccatattca caaaattttt
     3241 cttactactc gaaactaagc ggctatttca caaactcttg gtaaacggtg ttcaatcagc
     3301 taaccctcgg tattcgctca caaaacgaca aaatatgagg aactattttt ccgtttgttt
     3361 ctctaataat caggtcaagg tgctgattgc ccaacctttt tttgtataat gagaaagtat
     3421 aaacttaatg tttggaggag aagatatgtt agggaaggtt ttggaagagt tatttatccg
     3481 gatctgggtc attataaaac taaccttgta tttctggata tatacatttg ccggcgggat
     3541 catctttgga ttaggtgcgg catggaaaac agtcaatgaa ctattttatt tgtacggatt
     3601 cgagtataaa gaaatcacaa taaaaagagg ctggaatat
//
LOCUS       DAHHZJ010000197         3588 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.201, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000197 DAHHZJ010000000
VERSION     DAHHZJ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.201"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..310
                     /locus_tag="NBL02_002638"
     CDS             <1..310
                     /locus_tag="NBL02_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCD2573271.1"
                     /translation="LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVF
                     SSFSSFLKIIIGSGIILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE
                     "
     gene            303..2027
                     /locus_tag="NBL02_002639"
     CDS             303..2027
                     /locus_tag="NBL02_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCD2573272.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            2024..3475
                     /locus_tag="NBL02_002640"
     CDS             2024..3475
                     /locus_tag="NBL02_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD2573273.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         1303 a          580 c          709 g          996 t
ORIGIN      
        1 tttagctatt gattttattt tactttttgc cttgctctgc ctatttgcta cttatcaata
       61 cagtatgatt ttagatagtg aatatacgat ctctgttcct aatctgctaa agctaagttt
      121 tatttcggtt ttttccagct tttcgtcttt tctaaaaata attattggta gcggtataat
      181 tttaggcgtg acttggcaat tcaaaggatt gatcctgttt ggtgtcattg gtcttttgac
      241 ggtttggaat ggtacgatga caacacattg gcgtgaagaa ttagataaac agttggaaag
      301 ttatgaataa gtttcggatt ctattttcaa aaggaacctt actaaatcgt ctgttgcgga
      361 aatacgcggt tatcatgatc ggtgtaacga tgactgctac cgttattttt agtatccata
      421 cttgggaaca aaatcaaaaa caggcagaaa acatgacaag tgatgccgtt caaagcacaa
      481 gcagaatgct gaatgataaa acaaccttga gtaggatcat caaaaaccag cttgtaggga
      541 attccgaaaa gatcgaaaat gtgacgacct acttaacaaa acccatagat cagtatctga
      601 tgtatgtata cgaacagcaa aacagtaccg atgaattagt ttctttccca aatcaaatca
      661 aagatctcta cgcaaattat gaagaactaa gtgcgatata tatcgtgttg aatcaactac
      721 cggagtatta tgaatcttct agagaaaaca aaggtgggga aataaaaaag gggacaccaa
      781 aattactaga cgccttttat atcaagttgc ctatcacaca aggaggagct gagtcaggaa
      841 gtatttttat tggttttcaa aagaaagaac tagatatgat tttagaaaat ctaacgtcat
      901 tcaatggact ctctttatac atgatttctg gaacgacaaa tcgactatac acattccatg
      961 ataataaaat agctaaagaa acgtttgaaa gacaagaaat gatcatcaca gagagtctga
     1021 agaaaaattc acaattgccg attgacgatc tggaaaaaaa caattttgtt caacaccaag
     1081 aactgtctga tgattatcgt atattagctg tactagataa aaaaagtgta aaaatcgaaa
     1141 cgggaaaaaa tcttgccccg ctgatcattg gtatcattat tttagacaat atactgctta
     1201 tctttcttta taaaacattc aaacgatatt cacagcaagt atctatcgtc atgcaggcca
     1261 tggatcaaac agcagcaggg aatttagaga cgcggatcga cataacaaac acggaatatg
     1321 aactgaagga actttcaatc ggtatcaacg aaatgttgga tagcatcaat cagtttgtag
     1381 aagacatcta caaattagaa atcaaacagc aagacgcaca tatgcgggcg cttcaagcac
     1441 agatcaatcc acacttttta tataatacat tagaatatat ccgaatgtat gcaatcagcg
     1501 aaggaagtga agaattagca gatgttgtgt atgcgttttc cgctttgctt cgaaacaata
     1561 cgaatcaaga gaaaacgatc actttaaaag aagaactgga cttttgcgaa aaatatgtct
     1621 atttgtatca aatgcgttat cctaatcgag tagcctatca ttttatgatc gatcctgatt
     1681 tggaaaagat cgaagtacct aaatttgtga tccagccatt agttgaaaat tattttaaac
     1741 acggtattga ttttactcga ttcgataatg cgctaagtgt caaagtcttg caggaaggca
     1801 aacgggtaag gatcatcatc aaagataatg gaaaagggat gactgaaaaa cggctaaaac
     1861 agattgaaga aaaactgtct catcctaaag tggaacttca cggttctatc ggtctgcaaa
     1921 acgtgaatga acgtttacgt gccagcttcg gcagcagtta ttatatgtcg cttgagaata
     1981 atgagacagg tggactgacc gtttctatta cattcaagga gggatgagcg tgtatcaagt
     2041 gttattggta gatgatgaat acatgatttt gaatgggtta aaaaagataa ttgattggca
     2101 aagtttaggc tttcaaatag tagcaacagc agagaatgcc aaagaaggac tagctgtact
     2161 tgaacagcga cagattgatt tagtagtaac ggatgtgaca atgccagaaa taaatggact
     2221 ggaatttatt gaagcggctc aaaaagaacg tcataatttt gaatttatga ttttatcagg
     2281 ataccaagaa ttcgattatt tgaaaggagg tatgcaatta ggcgccgtga attatttgat
     2341 gaaacccgtg aataagttcg aattggttga aagcctcaag aaaatcaaaa cacggttaga
     2401 tcagcaaaat gaacaaaaaa atcaacaaga aatctatcaa gaactgttat tcagtcaatg
     2461 gttgaatgaa gaattggatg aaataagtga agaagaaata ataggacaaa taagtgagaa
     2521 gcagcatagg atagtattga ttcaactggc aagaactcat gatactctta tcaatagatg
     2581 gttgcaagaa cggcaacaaa tcttttacta tcaacgaaat tacggagacc gtgtattgat
     2641 cacgttgttg ttggaggcag atgaagttga tggcttctgt tgttttgttc aagaaaactt
     2701 tcctgatcag gaatggctga tcagtatcgg ggaagagaca gattctttgg acaaaatccc
     2761 tgaaacattc caacaagcga aagacaatct acagttgcat caattttatg gagataaaga
     2821 cgagcatgtt ttttacgcag tacaagcaga aacaaaagat caaaccatcg atttttcaag
     2881 tttcagacgc gtgctgcaaa ataaacaatt ggaagtcgct caaaaaatga tcactgattt
     2941 ttttgaacag tttcagttag cagccatgat gccggaagat atacgatatt ccagtttttt
     3001 actgtttatg gagattcaac gggaattgat tgatctggaa gatgaggaat atttacaagg
     3061 aatcgaaaaa atccatcagg caaaaacggt gcaagaactt cagcagctac ttctgtcatt
     3121 catcaaaaag catcagagac agaaaaagta cagtgacaat gtggaaaaag taatcgtaat
     3181 cctgcaccag cattatcaag aaccacttac actcaaagaa gtgtcagaaa acctgcattt
     3241 aaatgtcatg tatctaggac agctgttcaa aaaagaaacg aaaaaaagtt tttcagctta
     3301 tttgaaccat ttacgtatgg aaaaagcgaa acaactgctt ttgcacagta atcaaaacat
     3361 caatgagata gcaagtgaga ttggctacaa caacaccacg tatttctcta aattattcaa
     3421 gaaaatcgtc ggcagatccc ctaaagagta ccgagaaaac agtggagaat cataaaagaa
     3481 aaagccaatg taatgaaata acttataaaa aattgtctag aaatctaatt ttgggctgtt
     3541 cttattactt gaggcaaggc agctttttca tgacctctcg ataaacgg
//
LOCUS       DAHHZJ010000198         3563 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.202, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000198 DAHHZJ010000000
VERSION     DAHHZJ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.202"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            129..404
                     /gene="rpsP"
                     /locus_tag="NBL02_002641"
     CDS             129..404
                     /gene="rpsP"
                     /locus_tag="NBL02_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCD2573274.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            416..667
                     /locus_tag="NBL02_002642"
     CDS             416..667
                     /locus_tag="NBL02_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HCD2573275.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            792..1583
                     /locus_tag="NBL02_002643"
     CDS             792..1583
                     /locus_tag="NBL02_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573276.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            1753..2274
                     /gene="rimM"
                     /locus_tag="NBL02_002644"
     CDS             1753..2274
                     /gene="rimM"
                     /locus_tag="NBL02_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCD2573277.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            2264..3025
                     /gene="trmD"
                     /locus_tag="NBL02_002645"
     CDS             2264..3025
                     /gene="trmD"
                     /locus_tag="NBL02_002645"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCD2573278.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            3161..3508
                     /gene="rplS"
                     /locus_tag="NBL02_002646"
     CDS             3161..3508
                     /gene="rplS"
                     /locus_tag="NBL02_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCD2573279.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
BASE COUNT         1229 a          642 c          736 g          956 t
ORIGIN      
        1 gtctaaaatc ttctattttc taaaaaaaat cgaatttaat caagctgtaa agaaaaaaag
       61 ctttacaggg taaaaaaaaa ctgttacact acatcttgtg aataaaacaa tggaggtgga
      121 aataaataat ggcagttaaa atccgtttaa aacgtatggg ttctaaaaag agtccgtttt
      181 accgtatcgt cgtagctgat tcacgttctc ctcgtgatgg acgtttcatc gaaactgtag
      241 gtacttacaa tcctttgaaa gaccctgcag aagtagtttt aaaagaagac ttagttcttg
      301 actggttatc aaaaggtgca caaccttctg acactgtacg taacatcctt tcaaaagaag
      361 gcgttatgca aaaacaccac gaagctaaat tctcaaagaa ataaggtgac cggatatgaa
      421 agatttaagc gacttagtct taacaatcgt tcgtccgcta gtcacttatc ctgatcaagt
      481 caagttagac gtcgtagagt cgaaagactt ttacgagtac aatctaacag tggctcctga
      541 agatattggc cgtatcattg gaaaacaagg gcgtgttgct aaagcaatcc gcacaatcgt
      601 ttacggtgtt cggatcaatg caccgaaaaa agtgcgtttg aatatcatcg atgataagga
      661 aaagtaaata aacgtgctgt actcgaattt tcgagtgcag cttttttgtt ttttttatta
      721 attgaagaaa tgattatttg gcaaaacaag caagaaagcg tatccttata ggaggaggga
      781 gtgaacagcc aatgagaaag aaaatgttat ttattccatt tgctctattg ttaagcgctt
      841 gtagttcggt tactcaagaa tcagacaata cacatctatc aagcagtata tctgaaactg
      901 ttaccagtcc agaaaaaagt agtaccaaaa cggcttccac cactgaacaa acaatggcta
      961 cctcaaataa tgagaaaaag actgctttag accagttgaa agaacaacag ccaaatgttc
     1021 ccatgcctct agatgtacca gtctcttcag gctatctcaa tatcgcagcg acacatacaa
     1081 agcaaggtta ttctatttta tattacagaa cagatcgtcc cctcgggttg aatgctgacg
     1141 aattgaatca agaaacgcct attgcaacct atctatatca atatgggttt gcttctagtc
     1201 aagaaacaat ccaagttttg cagccatttg agatcgacac aaatggtcag caagtggact
     1261 taggttctcg aatcacaggg tatcagcaag gtgcagcagg ttcaagtttt cttgaatggc
     1321 aagaaggaaa ctggcgtatt cgaatccgtg ggaataatat agaaggccaa gatcccttac
     1381 tattagctaa agaaatcgta gcttatttgg aagaaaacag tctaccagca cctgaacaat
     1441 ttggtaaaat tactgttgat atgggtgata cgacgaatcg agccgtagaa gtaagctggc
     1501 aagaaccgaa aaatgcctat acgatcactc atcaagatcc tatgtctgcg ttgaagatgg
     1561 cagtttctat gaagaggttg tgaaatcagc gcgctgatct gagcccatca tttaactcca
     1621 agtcacagga agtaatcaaa aatagcacgc cgcatttttg cataattttg gcttaatacc
     1681 ttaaaagaaa actagaattg cctcttcaca catgctaaaa taaaacagac aaaaaagaaa
     1741 tggggatata agttgactga atacttgaat gtaggaaaaa tcgtaaacac acaaggtatc
     1801 aaaggcgaag tccgtgttat ctcaacaacg gatttcccag aagaacgtta caaaaaagga
     1861 acggtgctaa cactattcca agacggaaaa gcacctgtgg aattaacagt caaaagtcat
     1921 cgcaaacata aaaattttga cttattgagt tttgaaggac atccttctat caatgatgtg
     1981 gaaaaatatc gggatggcat attaaaagtg tcaaaagaca actctgtaga tctagctgaa
     2041 aatgaattct attaccatga gatcatcggt ctgcatgtat tagaagatgg aactgagtta
     2101 ggtaaagtaa aagaaatctt gtcaccaggt gccaatgatg tttgggtcgt tcaacgtccg
     2161 aaaaaagctg atatattgct tccatatatc gcttctgtcg tcaaagaagt cgatttagaa
     2221 gctggagtgg tccgtgtaga aattccagaa ggactgattg acgatgaaga ttgatgtatt
     2281 gactttattt ccaagaatgt ttgaaggtcc tatgggggaa tccatcatcg gtaaggctgt
     2341 agataaagga ttattagata tcaatatctc taacttccgc gattattcag ataacaaaca
     2401 tcagacagta gatgattatc catacggcgg tggagcaggt atgctgctca aagtccagcc
     2461 catctatgat aacatccaag ccattgaaaa agctgcaccg gatgtcaaaa aaagagtaat
     2521 tcttcttgac ccagctggta aacgattcga tcagaaaatg gcagaagaat tttcacaaga
     2581 agaacactta gtattcattt gtggtcatta tgagggatat gatgaacgta tccgcacatt
     2641 agtgacagat gaagtttcct taggagatta tgtccttact gggggagaat taggtgcgat
     2701 ggtcatgatc gatgcaacag ttcggctttt gccagatgtg ttagggaatc agacttcagc
     2761 tcaaacggat tcttattcaa ccggtttatt ggaacatccc caatatactc gcccggcaga
     2821 atataaaggg atgaaagtac cagaagtatt gacaaatgga aatcataaat tgattgaaga
     2881 atggcaatta aaagaatcac taagaaggac ctatcaacgt cggcctgatc ttttggaaac
     2941 acttgaatgg acacctcaaa tgacgaaatt tttagaagaa atcaagaaag aagaacaaga
     3001 aaaaacagca gatgaagcca agtgactttt tctaaaatct tctttacagt aggttgataa
     3061 tatgttagta ttttatggtg agtgcaaaag cacttaacta ttacgatatt ccgctgtgag
     3121 aaaagctcat aagaatattt ggaataagga gaagaaaacc atgaatccat taatcgaaga
     3181 attaacaaaa gaacaattac gttctgacat cccagcgttc cgccctggtg acactgtgcg
     3241 tgttcacgca aaagttgttg aaggtactcg tgaacgtatc cagttatttg aaggtgttgt
     3301 tatcaaacgc cgcggtgctg gaatcagcga aacttataca gtacgtaaag tttctaacgg
     3361 agtgggcgtt gaacgtacat tcccattgca tacaccacgt gttgcaaaaa tcgaagtagt
     3421 tcgctacggt aaagtacgtc gtgcaaaatt gtactaccta cgtgcattac acggaaaagc
     3481 tgctcgtatc aaagaaatcc gtcgttaatc atcgttatca gacagagaaa gaacccttgg
     3541 tattcaaggg ttcttttttt att
//
LOCUS       DAHHZJ010000199         3522 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.203, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000199 DAHHZJ010000000
VERSION     DAHHZJ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.203"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            281..601
                     /locus_tag="NBL02_002647"
     CDS             281..601
                     /locus_tag="NBL02_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HCD2573280.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGLLLISIIGIKI
                     TSGH"
     gene            762..1667
                     /locus_tag="NBL02_002648"
     CDS             762..1667
                     /locus_tag="NBL02_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD2573281.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSEGTIRIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVKQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDQAARTILTDAGYPLEDPDKIKLLKKNEELLGTVIQLLSAENVHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            1664..2437
                     /locus_tag="NBL02_002649"
     CDS             1664..2437
                     /locus_tag="NBL02_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCD2573282.1"
                     /translation="MTLLIKQELFKLIKKKSTAVLSVLLVALLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMESQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSIYLYLLAIIVTVLMKLILFPSISFTEKISTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIALFLALGYLVFKRKNV"
     gene            complement(2614..>3522)
                     /locus_tag="NBL02_002650"
     CDS             complement(2614..>3522)
                     /locus_tag="NBL02_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD2573283.1"
                     /translation="AISTFSIQQFINNFSSDIILNANGQPIPFSSQVLSCIMVMLWVF
                     VGIEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPA
                     MGYVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNK
                     NGAPTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQR
                     KEWVQFLVGFLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHL
                     DKILLIGVTMVGVIAMVLFATGMLHV"
BASE COUNT         1106 a          680 c          659 g         1077 t
ORIGIN      
        1 ctagttactg tttttttaaa tgattaataa ataacaactc ctttctatga aattgcaaag
       61 cattcatctt cttttctgct aacgatatat ccgtcaatgc ttgttacact ttactaaaaa
      121 gtatgagatt gccgatatct ggataggtgt gctatactac agaaaaagat atacgatctg
      181 ccaccgggta gtaaaaaaga ggatgatcat tccattaggt ctttgaaggg atgattctcc
      241 tctttttgtg tattttttat attgaaggag gaacgtatgt atggcatgga tggaattaat
      301 catagcagga attttggaag tattctggtc aacgatgatg aaatggagtg atggtttttc
      361 taaaatcaat tattcttttt acacagtgat tggaatgatt gctagttttt actttttgtc
      421 aaaggcaatc aaaagtttgc ctatgagtct ctcttatcct atttggacag ggattggcgc
      481 agttggttcg gtcattattg gtgtggtctt gtttcatgat aagctgaatc tttccacttg
      541 gttctttgtc gggttgctgc tcatcagcat cattggcatc aaaatcacta gcggtcacta
      601 gatacttaga caagcagaca aggactgatc agaaaaagcg gggcttttta tttgatattc
      661 ctctttctat gaaaaaacaa gaaaataaga ctaatgacct agcgacttct ctactcatca
      721 ggtgtatgct tgtactatct atagaaaagg agtgaatatt tttgaaaagt ctttcggtcc
      781 aagaattatc caagaacttt ggtcacaaac aagccctcag cgatgtcagt tttagcttat
      841 ctcctggcaa aatcgttgga ttagtaggtc ctaatggcgc tggaaaaaca acgatcatga
      901 aagctatttt aggactgatc cattactcag aaggaacgat ccgtattggt gaaaatgaga
      961 tttctccctc tagtcataaa ggattagaac aagtaggagc attgatcgaa tatccgggta
     1021 tctatccatt cttaacaggt tatgatcatc tgaagttatt ctctgaaaca aacgatgtat
     1081 ctgctatcga cactatcgtg aaacagctca agatggaaaa atacattcac aaaaaagcga
     1141 aatcctactc gctgggaatg aaacaaaaat tgggaattgc tcttgcctta ctgaaccatc
     1201 cgtctatcgt tattttagat gagccgatga acggtttgga tccacaagca acaagagatg
     1261 tgcgaaaaat cattaccgac ttggctactc agggcacctc tttcttgatc tccagtcact
     1321 tactcagcga gcttgaaaaa atggtcgatg agctgatcct gatcaatcaa ggaaaaatca
     1381 tcaaacaatg tacaatggct gaattgaacc aaacagcgca agatttcatc gttatctcta
     1441 cgacaaatga tcaagctgct cgaacgatct tgacagacgc tggatatccc ttggaagatc
     1501 ctgataaaat caagcttcta aaaaaaaacg aagagttatt aggaactgtt atccagttat
     1561 tgtctgcaga aaatgtccat gtgacagatg tccagcatct gacgaatgat ttggaatctt
     1621 ccctattgga aattctggat tcagtaaagg gggaagaaga aaaatgacgt tgcttataaa
     1681 acaagaatta tttaaattga tcaagaaaaa gtcaactgct gttttatctg ttctattagt
     1741 tgctttgctc ataggaacag cacttctagc caaaaaatac acgacgatca ttgatccagt
     1801 tgaaatgact gctcagcttt tcagtgccac atcttggatt gtctttatca tgattgctgc
     1861 tgcaagtacg atcatttcaa tggaatcaca atatggtaca ttgaaaaatc tcctttatcg
     1921 aaagtattct cgaggagaaa tattagtaag taaatggatc acattagtta tttattctat
     1981 ttatttatac cttttagcta ttattgtcac cgtgctgatg aaactgatcc ttttcccttc
     2041 gatttccttt acggagaaga tttctactgg tcagacactg atccaaagct tattcactta
     2101 tactttgggt agttatatcg gtttatggct aattttaagt ttagtgttga tgattgcttg
     2161 ttttatcaat agttccggtg cctccatctc agcagggatc gtcttttatt ttgcttcatc
     2221 aatcatatct ggtatcctca tcgcactcat tcaaaaatgg gagtggatca aatggaatcc
     2281 aatcagtatg ctgaatctgc aaaaccaaat tggcaacgaa gagattatga agcaattgac
     2341 tcatctttct actaatcaaa tgctgtttgg caatttagct tacattgctt tgttcttagc
     2401 attgggatac ctcgtattca agcgaaaaaa tgtatgatgg ttcattaaca aagaagttgt
     2461 gacagaagtg gccagtttca agaaatttcg gcttattttt agaagaagct acttcttgaa
     2521 caccatttat tcaaaaaaag aagcgatgac acgaatgttg tcaccgcttc ttttttgaat
     2581 aaaccacagg cttcacagcc tctctttata cttctatacg tgaagcatcc cagtggcaaa
     2641 taaaaccatt gcgattactc cgaccatcgt cacaccgata agtaaaatct tatctaagtg
     2701 gatatgttgt acaggttcgt tttgttcttt acgtgctttg aggtaaaaat aaatacctgg
     2761 taagaaaaga acaagaacaa gcatcacttc attaaaggct acaactagca tggcccatac
     2821 ttgaaaagca cttgctaaaa accctactaa aaattggacc cactctttcc gctgataaga
     2881 aagcttcatt tggtacagcc ctaagaaaag ccacgtgaag aaaatcgctg ctgtaccaag
     2941 tgtataagca aagttatatg cactgtcagt gaataataat gaaagtaaaa agacattcgt
     3001 acataacgtc gtcaatacga tagcataagt tggtgcccca tttttgttga gttttcccca
     3061 tgctttaggc aaaagtccgt cctcagccat attccccatt gtttcaatcg gcaagattgt
     3121 ccatgagagc caaaccccaa ctaaagaaat gatcaaaccg ccgttgatca cgaccgcccc
     3181 ccatttacca accatcattt caaatacata tcccatagca ggttgcgctt tgatcgtttg
     3241 cagctgattc atcgtcattg tgccatatgg aagcatggaa accaagacgt aaataatgat
     3301 caaaccgaat attcctagaa tcgttgcttt cccaacatcc gattgttttt ttgctcgtga
     3361 acctacgaca gaagctcctt caataccaac gaatacccag agcatcacca tgatgcaact
     3421 caatacttga ctagaaaacg ggatcggctg cccattcgca ttcaatatga tatcgctaga
     3481 aaagttatta atgaattgtt ggatcgaaaa ggtgctgata gc
//
LOCUS       DAHHZJ010000200         3471 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.204, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000200 DAHHZJ010000000
VERSION     DAHHZJ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.204"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..1000
                     /locus_tag="NBL02_002651"
     CDS             <1..1000
                     /locus_tag="NBL02_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCD2573284.1"
                     /translation="ITGGTGSFGNAVMKRFLDSDIKEIRIFSRDEKKQDDMRKKYNND
                     KLKFYIGDVRDINSVKNAMYGVDYVFHAAALKQVPSCEFFPMEAVKTNIMGTDNVVTA
                     AIEMGIQKVICLSTDKAAYPINAMGISKAMMEKVFVAKARTVTDERTMICGTRYGNVM
                     ASRGSVIPLFIDQIKNGKPLTVTDPGMTRFLMSLEEAVELVVFAFQNAKAGDIMVQKS
                     PASTVGDLAQAIKELFHADNEIHIIGTRHGEKRYETLLTKEEYLVAQDMGDFYRVPAD
                     TRDLNYDKYFVEGSKELTQEGEYNSDNTQRLSVEQIKERLLELDYVRKELKEWQNQ"
     gene            997..2106
                     /locus_tag="NBL02_002652"
     CDS             997..2106
                     /locus_tag="NBL02_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CapF"
                     /protein_id="HCD2573285.1"
                     /translation="MNILVTGSNGFIGKNLVAELKNEGFTDILSFDRDTPVHLLDEYC
                     EKADFVFHLAGVNRPKDDAEFMTGNFGFTSELLEKLKEHGNTCPVMISSSIQAELNNS
                     YGKSKKAGEDLLKKYQKETGAPVYIYRFSNLYGKWSQPNYNTVVATFCYKIARDEEVT
                     VNDPSAIIELCYIDDVVKELLHCLNGQPYTENGYCAVRETDEVTVGELKELITSFKNS
                     RTNLSVPNLARRVEKNLYSTYLSFLPEDEFSYFLKMNVDNRGSFTEFLRTKDRGQVSV
                     NISKPGITKGNHWHHTKNEKFLVVSGEGVIRFRKVDSNEIIEYFVSGEKLEVVDIPVG
                     YTHNIENLGSTDMVTVMWVNEPFDPEHPDTYYVEV"
     gene            2110..3234
                     /gene="wecB"
                     /locus_tag="NBL02_002653"
     CDS             2110..3234
                     /gene="wecB"
                     /locus_tag="NBL02_002653"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCD2573286.1"
                     /translation="MKKLKVMTVVGTRPEIIRLSAVINRLDRSEAIDHILVHTGQNYD
                     YELNEVFFEDFKLKKPDYFLNAATGTAIETVGNILIKIDPILEEVKPDAFLVLGDTNS
                     CLCAIAAKRRHIPIFHMEAGNRCFDQRVPEETNRKIVDHTADINLTYSDIAREYLLRE
                     GLPADRVIKTGSPMFEVLNSRKDDIQHSDVLNRLGLKEGQYFVVSAHREENISSETNF
                     LHLVDSLNTIAETYQLPIIISTHPRTMKMIEAKEIKFHELIQTMKPMGFNDYNKLQIN
                     AKAVLSDSGTISEESSILNFKALNIRQAHERPEAMEEASVMMVGLEKERIMQGLEVLE
                     TQQKDTLRHVADYSMPNVSEKVLRIILSYTDYVNRVVWGR"
     gene            3231..>3471
                     /locus_tag="NBL02_002654"
     CDS             3231..>3471
                     /locus_tag="NBL02_002654"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HCD2573287.1"
                     /translation="MRIVVNDIAASEGGAMSVLRDLHSDIISSGDENEWIFLLGEKFI
                     EESDNIKVLTFPKIKTSWIKRLDFDFISGKKIINKL"
BASE COUNT         1251 a          553 c          713 g          954 t
ORIGIN      
        1 aatcacaggc ggcacgggtt cttttgggaa cgcagtgatg aaacgctttt tagattcaga
       61 tatcaaagaa atacgtatct tttctcgtga cgagaaaaaa caagacgata tgcggaaaaa
      121 atacaataat gacaaattga agttttacat tggagatgtt cgggacatca acagtgtcaa
      181 aaatgcgatg tacggcgttg attatgtctt ccatgcagca gcacttaaac aagtaccttc
      241 ttgtgagttt ttccctatgg aagctgtgaa aacaaatatc atgggaacag ataatgtcgt
      301 aactgcagca atcgaaatgg ggattcaaaa agttatttgc ttgtccactg ataaagctgc
      361 ttatccaatc aatgctatgg ggatttctaa ggctatgatg gaaaaagtat ttgttgctaa
      421 agcaagaacc gttactgacg aacgcacgat gatttgtggc acacgttatg gaaatgtaat
      481 ggcttctaga ggatcagtta taccattatt cattgaccaa ataaaaaatg gtaaaccatt
      541 aacagtgaca gatcctggca tgacaagatt tttgatgagt ttggaagaag ctgtagagtt
      601 agttgttttt gccttccaaa acgcaaaagc aggggatatc atggttcaaa aatcaccagc
      661 ttctactgtt ggcgacctag cgcaagccat caaagaatta ttccatgcag acaatgagat
      721 tcatatcatc ggaacgcgac atggcgaaaa gcgttatgaa acactactga caaaagaaga
      781 atacttagtt gctcaagata tgggtgattt ctatcgtgtt cctgcagata cacgtgattt
      841 gaattatgac aagtatttcg ttgaaggaag caaagagttg acacaagaag gagaatacaa
      901 ttcagataat acacaacgtt tatctgtaga acaaatcaaa gaaagacttc ttgaactgga
      961 ctatgtaaga aaagaattga aagagtggca aaatcaatga atattttagt aactgggtca
     1021 aatggtttta ttggaaaaaa tttagttgct gaattaaaaa atgaaggatt cacagacatt
     1081 cttagttttg atagagacac gcctgtacat ttattggatg aatattgcga aaaagcagac
     1141 tttgtttttc atttagctgg agtgaacaga ccaaaagacg atgctgaatt catgacagga
     1201 aattttggtt ttacttcaga attgctagaa aaactaaaag agcatggcaa tacatgtcct
     1261 gttatgattt cctcttctat ccaagcagaa cttaataatt cttatggcaa gagcaaaaaa
     1321 gccggagaag atctattaaa gaaataccaa aaagaaacag gagcacctgt atatatttat
     1381 cgcttctcaa acttatacgg taagtggtcg caaccaaatt ataatacagt tgtagccaca
     1441 ttttgttaca aaatcgcaag ggacgaagaa gttacggtaa atgatccgtc tgctatcata
     1501 gagctttgtt atatcgatga tgtcgtgaag gaactactcc attgtctgaa cggacaacct
     1561 tatacagaaa atgggtattg tgctgttcgt gaaacggatg aagtaacggt cggcgaatta
     1621 aaagaactaa ttacatcttt caaaaatagt cgaacgaatt tgagtgttcc taatcttgcg
     1681 agaagagtag aaaaaaatct gtacagtacg tacttaagct tcttaccaga agacgaattt
     1741 tcttattttc taaaaatgaa cgtagacaat cgaggatcgt ttactgaatt tttaagaaca
     1801 aaagaccgcg gtcaagtatc tgtcaatatt tccaaaccgg gaatcactaa aggaaaccat
     1861 tggcatcata caaaaaatga aaaattctta gtagtaagcg gagaaggtgt aatccgtttt
     1921 agaaaagtag actcaaatga aataatcgag tattttgttt caggagaaaa attagaagta
     1981 gttgatattc cagtaggtta tacgcacaat atcgagaatc tgggatcaac ggatatggtg
     2041 acagtgatgt gggtaaatga gccatttgat ccagaacatc ctgatactta ttatgtggag
     2101 gtctaatcaa tgaaaaaatt aaaagtgatg acagtagtag gaacccgacc agaaatcatt
     2161 cgtttgtctg ctgttatcaa ccgtttggat cgatcagaag caatcgatca tattttagta
     2221 cacactggcc aaaactatga ctatgagcta aatgaagtat tcttcgaaga cttcaagcta
     2281 aagaaacccg attattttct aaatgcagca actggtacag cgattgaaac agttggaaat
     2341 attttgatca agatcgatcc aattttggaa gaagtaaaac cagatgcatt cttagtatta
     2401 ggggatacaa atagctgttt atgtgcaatt gcagccaaac gtcgccatat tcctattttt
     2461 catatggagg caggaaatcg ctgtttcgac caacgagtac cggaagaaac aaatcgtaaa
     2521 attgttgatc atacagctga tattaatcta acctatagcg atatcgcgag agaatatctc
     2581 ttacgtgaag gattaccagc tgatcgtgta atcaaaacag gaagtccgat gtttgaagtt
     2641 ctcaattcaa gaaaagatga catccaacat tccgatgtcc ttaatcgctt agggttgaaa
     2701 gaaggacaat attttgttgt ttctgctcac cgcgaagaaa atatcagctc agaaacaaat
     2761 ttcttacact tggtagatag cttgaataca atcgctgaaa cttaccaatt gccaatcatc
     2821 atcagtacgc atccgcgaac aatgaagatg atcgaagcaa aagaaatcaa attccatgag
     2881 ctgatccaaa caatgaaacc aatggggttc aatgattaca ataaattgca aatcaatgca
     2941 aaagctgtac taagcgacag cggaacgatt agcgaagaat cttccatttt gaatttcaaa
     3001 gcattaaaca tccgtcaagc acatgaacga ccagaagcaa tggaagaagc aagtgtcatg
     3061 atggtaggat tagaaaaaga aagaatcatg caaggactgg aagtattgga aacgcagcaa
     3121 aaagatactt tgcgacacgt cgcagattat tcaatgccaa atgtttcgga aaaagtattg
     3181 cgtatcatcc tttcgtatac agactatgtg aatagagtgg tgtggggaag atgagaattg
     3241 ttgtaaatga tatcgctgct tcagaaggtg gggcaatgtc agtattgaga gacttacatt
     3301 ctgatataat aagtagtggt gatgaaaatg aatggatttt tttactaggt gaaaaattca
     3361 tagaagaaag tgacaatata aaggtactaa cttttcctaa aataaaaact agctggatta
     3421 aaagattgga ttttgatttt attagtggaa aaaaaattat caataaatta c
//
LOCUS       DAHHZJ010000201         3332 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.205, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000201 DAHHZJ010000000
VERSION     DAHHZJ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.205"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            52..1740
                     /locus_tag="NBL02_002655"
     CDS             52..1740
                     /locus_tag="NBL02_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573288.1"
                     /translation="MKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLSRI
                     KGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLLNI
                     CTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYY
                     AYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFV
                     VVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDI
                     TTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLL
                     QFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVI
                     QSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKA
                     ADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIE
                     ITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            1727..>3332
                     /locus_tag="NBL02_002656"
     CDS             1727..>3332
                     /locus_tag="NBL02_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573289.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCL"
BASE COUNT         1140 a          487 c          552 g         1153 t
ORIGIN      
        1 gacaattcta gtatactgaa aatagaatta aaaaaggaga acaacaaatc tatgaaaata
       61 agtaaaaaag catttctttt agtagttctt atcttactta gtactttata ttcagtaaat
      121 tttatgagaa atgctcaaga aatttataca acaggtgatt tatcttttca tctttcacga
      181 atcaaagggt tatcttctat ttttgaaggg ccaatcaact atacaacatt taacaattat
      241 ggagatggac tgaactattt ttatcctttt cttacaataa ttcctgcagt cgttttttat
      301 ggaatttcaa ataacttaat attaagttat gttctatata tttggttatt aaatatttgt
      361 acgattctga ttagttttta ttatggagaa aagtttttta aaaagataga tgctgctttt
      421 ttatttagtt gtttatatac ttttgcaact tatcgaacag ttgatatcta ttatcgatcc
      481 gcaattgcag aagcaatcgc tcttacgatt atcccagctg ttttatatta tgcttatgga
      541 ttgatattca atagagaaaa aaaagtcatc gctttatctg tcagtataag tctacttgtg
      601 tatactcatg tcttaactac actattgtgt agtgcattga ttgcttgtat gattatagtt
      661 cgtttgtttt taagcaagca aataaaaaac gatataaaat ttgtggtcgt gaatttagta
      721 aaggctgttt ttttgacttg tgcattaact agctattttt ggtatcctat gttgcagcag
      781 atgaattatc aagaaataca cagtcccttc aaaacagatt tgcagctaga agcactttct
      841 atttcagata gtatcattca agcgttaaat aacgatatta caacatacac tatggggtta
      901 ttaggattgg ttgcgttgat ttttccttta ttcatactga aagaaatgga gaaaaaagaa
      961 aaaatcatct atgttggcgt agtttctaca tgggtatgcg taactaattt atttccatgg
     1021 tttttgtttc aaaaaacacc gataagctta ctccagtttc cttggcgtat cttaggtatc
     1081 caaatctttt tttcttcaat gatcataacg cttgttttta tgaaaaagac tatgaataaa
     1141 aaatattcat ggttaatcat tggcgctact gtggtgtttc tactcgtcac gtctatagca
     1201 gcaaaagaaa attacgcaaa agttatccaa tcaaaacacg gtcatattgt tatcaatgaa
     1261 gaaacactcc cacgttatac aacatcagat atgggaggat tatacgatta cgccccaaca
     1321 gaagcgataa aagaaaggcc tcacttagaa aaacatgaag taaaagtagg agaatcgtgg
     1381 gaaaaaggga agtataaagc tgcagataat aacattactt acacagtggc aagcaacaaa
     1441 aagcaaaaag tgacagtacc agtatatgct tatttaggta cacaggtaaa agtaaacggt
     1501 aaagtcgtaa atgactctta taaaaaacat ggcttagtga atgtggatgt aaatgctgga
     1561 gaaaataaga tagaaattac aacaaaatat actccaactg ctctcatggc tttatggatc
     1621 agtattctga cttattgttt agtggtgtat tcaagtttta aacgatttgc acctaataag
     1681 ttgaaagatt ctcgatatgt gactaaaaag gagataaaca agaagaatga aaataaataa
     1741 aaaagtagta atattgagtg gattaattat agttagtagt atatattctt tcatcttttt
     1801 caaaaataca cagagtattt ataccactgg agatctttct tttcacttat ctcgaatcaa
     1861 ggggttatcg actattttta ccagtccgat taattatgag acattcaatt atactggata
     1921 tggtgtaaat tatttttatc cctttctgac tttttttcca gctgtaatgt tatattggat
     1981 gacaaaaaat ttgattgtaa gctatattat ttacgtttgg ttattgaact tgtgtacaac
     2041 actcgtagct tatcattatg gcgagcggtt tttaaaacaa aaaaaagcgg catttttatt
     2101 tagttgtctc tatattttct ctgcttatcg gacagtagac atctactatc gttccgcaat
     2161 tgccgaagct atcgcaataa cacttgttat ccctgtatta ttttatgctt atcaaatcat
     2221 ctctggaaaa gaagaaaagt atccgtctgt aaagctagca ttgagtatga gtttgctagt
     2281 gtacagccat gtactatcaa cattaatgag tacagcatta attatcattt ttatttttat
     2341 tcgtttggtt tctaaaggtt tcaagaatgc agattttatt gctatattta aaaaattatt
     2401 tagtgcggca ggaatgacat tggttctgac gagtgctttt tggtatccga tgtttgaaca
     2461 aatgctctac caaaaaatca acaaacctag cgtgacaaat ttatatgctc atgcatctaa
     2521 tgtgttcgat agtctgactg aagctatgaa caacgactta actacttact caatgggact
     2581 agttggtctg ttatctttat gtattcccct gattttattt aaaaaattaa caaacataga
     2641 aaaaaagatt tattatggta cgtgtttgac ttggctagct acaactagtt tggtgccatg
     2701 gtatcttctg caaaatacgc cagccaaatt gttacaattt ccttggcgga tcttaagctt
     2761 gcaaataata ttctcttctc tgatattaac gatgatattt ttcaaaaata gaagatacaa
     2821 taagacaaga gaactatttt atttatttat gactgttttg ttattaatca ctttaactgt
     2881 ttctagtaaa aaaaatttca atcaaaaaat aatttctcag ccgggacata ttgttgtaaa
     2941 caaagaaaca gtagaggcgt ataccactaa aggaatggat tattttttat atgattattc
     3001 tcctatcgct tcattggaga ataaggagca tataataaaa cacgaagcaa agattggcag
     3061 tcaatgggta aaccttcctt ataaaacaac tggtagttct atcatatata gagtaactaa
     3121 tgagaagagc caagtaatca cacttcctgt atatgcttat ttaggaacga atgtagaaat
     3181 aaatcatcaa cctgtaaagg agcaggtgaa tgctaatgga ttggtacaaa taagcggagt
     3241 caaaggagtt gctgatatta aagtaagcac aagttataca gtaacgacta ttatttctta
     3301 tcttgttaca ttaactgcat atttatgttt at
//
LOCUS       DAHHZJ010000202         3318 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.206, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000202 DAHHZJ010000000
VERSION     DAHHZJ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.206"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(<1..2232)
                     /locus_tag="NBL02_002657"
     CDS             complement(<1..2232)
                     /locus_tag="NBL02_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573290.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="NBL02_002658"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="NBL02_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          907 a          744 c          536 g         1131 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccata
//
LOCUS       DAHHZJ010000203         3191 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.207, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000203 DAHHZJ010000000
VERSION     DAHHZJ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.207"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(<1..234)
                     /locus_tag="NBL02_002659"
     CDS             complement(<1..234)
                     /locus_tag="NBL02_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCD2573291.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSD"
     gene            complement(713..1432)
                     /locus_tag="NBL02_002660"
     CDS             complement(713..1432)
                     /locus_tag="NBL02_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCD2573292.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1481..3127)
                     /locus_tag="NBL02_002661"
     CDS             complement(1481..3127)
                     /locus_tag="NBL02_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCD2573293.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
BASE COUNT         1005 a          693 c          550 g          943 t
ORIGIN      
        1 atccgaagaa aataaaaatt tgaaattttc ccaacccacc caaggacttt ccctcaagct
       61 agccaagaac ccatctgctg aaatatgaaa atttttgaat gctacgacat tcgccattac
      121 aggtatgtaa aagaaaaaaa tcatccaaag catacccggt acagccatca ggacaagtgc
      181 tttataacgc catagattca taaaaaaatg attgaacccc tttcgttttt tcatctaaaa
      241 acccctttcg attcattcgc aacgtaatta tagcgctttc aataatgaga ataaataaac
      301 aaagtatagg gaaaatgaaa caaagtatag gagaggttgt gaaatcagcg ttttggcctg
      361 agtattagag aaaaaaacgg gaaaatagtt cctcatattt tgccgttttt tgagctaata
      421 ccgagggtca gctgattgaa caccgtttat cagaggttgt gaattcagcg ttttggcctg
      481 agtattaggg agatcgtgtc cttactatct aactctgagt cacaaggaac aatggggact
      541 gtaacaaaag tcttgtcaca gtcccttatt ccgaacaaac ggtgttccag aagtagcttc
      601 tacggaaata agccgaaatt tcacaaaaaa gccatccgaa agtcagacgg acaagtctgc
      661 ttttcagatg gcgctacaac tactctgctt attcttttag ctatccattc aattattttt
      721 ctctcacttg ttctagctct tctttggtca tgggtcgata ttgcccttta ttcaaatctt
      781 taggcagcca aaattttccc atacggattc tttttaaata taagacttct tttcctactg
      841 ctttgaccat tcgcttaact tggtgaaact ttccttcatg caaaatcaga cggataattg
      901 aagtttctgt ctcttgatct acttgatcaa tttggagttt tgccggcaat gtctgctcat
      961 cgccatcaat aatcagtcct tctgcaaata aacggatatc ttcttctgtc attataccgc
     1021 taacctcagc tagatattct ttctccacat gttttttagg tgatagtaac tgatgggcaa
     1081 acaccccgtc attcgttaaa atcaagaggc cctctgtgtc tttatctaat cggccgactg
     1141 gaaaaagatc ttcccgataa tcctggtctt ccaataaatc gattacggtt tgatcatgat
     1201 tatcctgagt agcagaaatg actcctgcag gtttatgcaa aatgtagtaa aaatcttttt
     1261 gatacgtaat gatttctcct aaataagtaa ccgcatcttc gaattcattt acttgcgctt
     1321 tatcgctttt caccacttgt ccattgactt cgatcgttcc ttttttgatt aatcctttga
     1381 cttctttgcg gctgccaagt cccatatctg ctaaaaactt atctaaacgc atgcgtttcc
     1441 cccatttcaa aagagcatca atctagctct tctgattcac ttattttatt tttaatttat
     1501 gtcgtaaacg cgctattcct ggtcctagta gtctttctgc caagcggatt ttcaaagcta
     1561 aataaccata cactaaagcc cctatacctg ctactacaag aatcaacaat agagactgga
     1621 atttgctatc ttcgtttaaa aaagcaccaa aaatcatctt tgtgatccac gaagccaaaa
     1681 gcataatcaa ggtaagaata aagatcagca atgtcctgcg ccagacaaaa cttggtttaa
     1741 agcgggctac ttcatggatt ctctttaaga tcaaggtaca agacacagca aatccgatca
     1801 ttgtcgccaa taaagggccg tatacttcaa ataaacgaat ggctgggtat tgaagaagga
     1861 gtttcactag aaacccaata accaaataat taattgccgc cttgttttca aacattcctt
     1921 gcagcatgtt ggaaactagc atatatagtc ctaggaagag tcctacaaaa cttgcttgga
     1981 tcagtacatt acttcctaga gaatccggtg tataaaataa tgtatttagc ggataggcaa
     2041 gtaacataac accaaatgtc gccggaaaca taaaaaatga aaacaactgt aaatttccgc
     2101 ttgtcaattt tgccagacct actcgatctt tcaacgtaac agcttctgta atcaacggta
     2161 acccagtaga cgcgatcgat gttgccaatg caataactac catcgtcaac ttatctggat
     2221 tggcactaaa aatcgaaaaa agatccagta actgtgcatt ggaatactcg gtggatgcag
     2281 acatgatctt cataaaagtg aactgatcga ctaatttgaa gatcgttacc cctgaaccca
     2341 caataataaa cggaatcgct tctttaacgg tatctaacaa caattccttc gttgcaatat
     2401 ttattttgtt ttcgctgtag tgaataaacg ccgatgtata cgcttgatgt ttcttcaaaa
     2461 aatacaaaag gacaagaata cttgctagca taccgataaa tgccgcgaaa gttgattgcg
     2521 tgaccgccgt cacatagtca ccatccatga ctttcatgat gatgaatgta gccaatagca
     2581 tataaaaaat acgtgccacc tgttctacaa tctgcgacag cgcataaggc atcatttctt
     2641 gattgccttg caaataccct cgtatcacac tcatacaagg aaaaaccaat acagcgatac
     2701 tcaaagaacg catcgttgga atcagttctt ctccgccacc agaagcatga gctagccaag
     2761 gagaggcgat gtacatgaac aaagcaaaca acaggcctaa cccgcccatc atctgtaatg
     2821 cacggataaa taatcgtcgt gaagtgccat attcattcaa tgaattatag cgtgcagttt
     2881 gttttgcaat cgcagcaggt attcctgctg tagagatcag tagaaacaat gcatatatat
     2941 tgtatcccat attgaataat ccattggcag ccttagcatt ttcgcccatc caagcatacc
     3001 agggaatgat atagacagcg cctagcaatc ttgaaatgat attactggcg gtcatccaag
     3061 cagagcctcg tgccattttt tcttgcggtg ttaattgatc taatgctggt tttctttgat
     3121 tatccatcgg tcagattcca actttctaca aaacattact atcattttaa ttggtaaaac
     3181 agaaactggc a
//
LOCUS       DAHHZJ010000204         3187 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.208, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000204 DAHHZJ010000000
VERSION     DAHHZJ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.208"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(16..1770)
                     /locus_tag="NBL02_002662"
     CDS             complement(16..1770)
                     /locus_tag="NBL02_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2573294.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLIKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(1880..2449)
                     /locus_tag="NBL02_002663"
     CDS             complement(1880..2449)
                     /locus_tag="NBL02_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HCD2573295.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(2467..>3187)
                     /locus_tag="NBL02_002664"
     CDS             complement(2467..>3187)
                     /locus_tag="NBL02_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD2573296.1"
                     /translation="NALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
BASE COUNT         1069 a          600 c          413 g         1105 t
ORIGIN      
        1 aaagcaaatt tgtttttata aaaacttttc attgtcaagc ctccaatcat ttgcattctc
       61 ataattagtg accaattgtt tatacacttg atgtttagcc attaattcat tatgatttcc
      121 acgttctact attttccctt ctgacaagac aagaatttca tctacagatt taatgctgga
      181 taatcgatga gctatcataa taactgtttt ttcttttatc agatgttgaa aagccttttg
      241 tagttcatac tcattatctg gatcaataga tgcactggct tcatccataa taacaatctt
      301 tgcatctttt aaaattgctc tggcaatagc tatacgttgt ttttctcctc ctgacagata
      361 aactccttta ctaccaatta aggtatgctc tctttcggga aatttttcta aaatcgattc
      421 gcaaccggct aatcttagcg cgtcaaatac tttacttgct ggcgcttgag gatttgctat
      481 agaaacgtta tcgtaaatac tctgattgaa tagtttcgta tcttgaaaga caaaagcaat
      541 tgtgttaata attgtgtctt ttgaatagtg atttaaaggc ttaccaccaa ttttaatctg
      601 tcccttatcc acattgtaga aacctgaaat tagtttagct atcgtactct ttccgcttcc
      661 tgattttcct actaatgcat aaattctttt ttgttctaaa actaagttga aattttctaa
      721 aacacactct tctttgtatg aaaagctgac attttcaaat gcaatgtcac aatttttaat
      781 ctgtgtctcg ttcccatatt ttagttggtc ttgcttcatt tctaaataaa gggagtctag
      841 tgtttcgact gcatatcttg cttcaaacaa gtgcatactt acatacatta ttctcatgaa
      901 agaaacaaaa ataattccac ttaaaaaaaa caacatcaac aagtcaacag ctaatatctt
      961 gggagtggta agagaagtta taaaaaaggt tataggaact atcaaaatgc aaactatgcc
     1021 aaaaaatagc cattgaaaaa gaacaaaagg tgttttccca ctcaaagaat acttatatgc
     1081 aaacttagca taatcgttaa ttgctttatt caaggcttta aatgatgtta ctttagcacc
     1141 aaatatcttt ataacttgaa ttccgcggat atattctacc gtttcagagc ttaacacatc
     1201 taacgattct tgatattttt gaataaaatt tgtattctct gtcattttct ttaacaagaa
     1261 tacaccaatt actaacatgg cgattagaac caatccaatt ttaaagttaa tgaaaaagcc
     1321 taaggcaact gataaaacag gtgttactat ggcttgagca ctatctggaa tcaagtgagc
     1381 aattgccata tgtgttttag ccgcgttgtc atcaattgtc tttcgaatat atcccgaaga
     1441 atttaaatca aaaaaacgaa aacttgcttc tgttaaaccg ctaattccct tttttctcaa
     1501 attagtctct aagcgaaacc caaggatatg agaaaataag ccagaaaaaa tataaaataa
     1561 agctcctaaa gttaaccaag caacagtttg aaccgcataa ctcttagcta aatattcatc
     1621 tcctacaata attagagact gaagaaattt gtaaatactg taatatccat agactgttaa
     1681 tacgacagaa aaatcagaaa ataaagtggc taaccatccg tagtatttca ttttagggac
     1741 ataactaaat aattttttgt atactttcat tttttcctcc cccgcattca atttcttctc
     1801 ttattcgttt acaactgtta atgctttccg taatagcgta aattagagat gtacaaacag
     1861 ataaaaggta aactacgttc tattttttgt atttttttaa gacttttctt ccaatcaata
     1921 caccaatcat agataaactt gctacaacta acactgaaat gattccccat atacctaccg
     1981 catagtggat cattgcatcc attgttgcag cagattgtcc tttatctatc caatcttgac
     2041 gaaaacgatc tgtaaagaac catataggaa tccactgtcc ggcagcactg cctaattgca
     2101 ttagagccca agacgcaact tgaggaattg ctcgatcata tccaaataca gatagaataa
     2161 tatcagctaa gatcgccata ctaatcgaac tgataaacca aggtaagtac gccccgccca
     2221 ttaacgtaaa aaataacaat aaaacaattg tgtaaatagt aaaaattcct tttctagggt
     2281 agttatagct aatataaaca aatatgattc cagataataa tccaaaaatc ccgggggata
     2341 tgctgtgacc aaacgcccca ccaatactac taaccattat tgaaaaaaag taaattacta
     2401 gggaaataac tgtagctaaa ccaataaaaa tataatcttt tgttttcata tatattctcc
     2461 tcaattctat ttcgttatgt cattaaaaat ctgttctatc aaagttgaat cttctactct
     2521 aagttttata tcttttttta ttccaccatt ttttaaataa ataattctat tgcatgtttt
     2581 ataaagaaat tccggatcat gagtagctat aactactgca cccttttctt ttaagtcttt
     2641 aataaggtca gcaatccttt ccatattaat ataatccaac ccacttgtag gttcatcaaa
     2701 tagaaataaa ttagtttttg acaatgaagc tatagaaacc aataatcttt gtttttctcc
     2761 accggataaa tcaaacggta attgatcttt cattttataa agttccattt tttttaaaat
     2821 tttttcttgc tgatctttac taactatttc attccccagg gctaactcgc tcctcactga
     2881 ttctgtgaaa aattggtaat ccatttcttg cattactaaa aacggcgatg acaaattttt
     2941 tttattttcg attatctttc ctttggtagg gcgaataatc ttcatgagta ttcttagtaa
     3001 ggttgttttc cccacaccgt ttttccctac caaaccaata acatctccaa ctttgacatc
     3061 aaaatttatg tcttttagaa tattttcata agtaatttct tcaactttta atacatctgc
     3121 acttaccttt tcgatatagg gaacttcaat cgaactatta aaaatgtcaa atttccttaa
     3181 tgcgtta
//
LOCUS       DAHHZJ010000205         3181 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.209, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000205 DAHHZJ010000000
VERSION     DAHHZJ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.209"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..212
                     /locus_tag="NBL02_002665"
     CDS             <1..212
                     /locus_tag="NBL02_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD2573297.1"
                     /translation="MEKQENFPIYIIYLHFYRKSYNYHLIEFMYQHPEKNLEDYADAQ
                     YTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(378..1571)
                     /locus_tag="NBL02_002666"
     CDS             complement(378..1571)
                     /locus_tag="NBL02_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD2573298.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            1795..2172
                     /locus_tag="NBL02_002667"
     CDS             1795..2172
                     /locus_tag="NBL02_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCD2573299.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            2160..2498
                     /locus_tag="NBL02_002668"
     CDS             2160..2498
                     /locus_tag="NBL02_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCD2573300.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            2632..3075
                     /locus_tag="NBL02_002669"
     CDS             2632..3075
                     /locus_tag="NBL02_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HCD2573301.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
BASE COUNT         1152 a          608 c          592 g          829 t
ORIGIN      
        1 ctatggaaaa gcaggagaac ttcccaatct acattattta tcttcatttc tacagaaaat
       61 catataatta tcatttgatt gaattcatgt atcaacatcc tgagaaaaat ttggaagatt
      121 atgcagacgc tcaatatacg agtgtatcca cagtttttcg ttatgctaag ttattagtaa
      181 agtactttga aatgacggac gtcaaagaat agtcgccgcc agatagaagg aaattagcag
      241 ttgaaaaagc tgccctgcat caagtaatac gaccagccaa acggaaacag tgtctcatgt
      301 ttttgatttg gctggtctta ttaccgcaga tgcaagcttt tgccacacct ctttcttgtc
      361 atttacagac tcaactatca atgctgcaag ctttccctta atgctgtaac accagtaaga
      421 gttgtcaaga ccagcagact gactaggtaa ataccgctca aaaacaagat tacggcagtt
      481 aaactataat gatccatcaa gaaaccgatg acgatagaag aaaaaccacc aacagctcgt
      541 ccaaggttca taatgaaatt attcgccgtc gcgcggattt ctgttggata caaactacta
      601 atcactgcgc catatccacc atacatgcca ttgataaagt agccgactat cacagcggct
      661 gctaccaaac tccactggct atgggctaac acaagtagat agacagataa ggatgaacaa
      721 attaaaaaca aagaaaaggc tagtttaggc cccaatttat ccatgattat gccaaaagtc
      781 atcatcccca aagacatgcc taaaatagta ctgaccatcc acaatgaaga cccagaaacc
      841 gaaagcccta atcgattttg cataattgaa ggcagccaat tcattaaccc aaaatagccg
      901 gcaatttgga cagttaccat gatactaagg ccacatgttt gccaagcaag atgttttgat
      961 tcaaacagtc tgctaagttt ggttttcttt tcggcagatt gtacagattt gaattccttt
     1021 ggttcagtca agccgcgacg aatgaacaag accatcaaca ccggcagtac acctacgaca
     1081 tacaacatct tccaaccaaa cgttggaatg atcacagcag ctaaaatcgc agccaaagcc
     1141 gcaccgacct gcgcaccaat accagcaacc gaagaagctc gtccaatttg tttttttgaa
     1201 aaactttcgg aaatcaagga catacacgcc ccgtattctc ctcccgctcc tacacctgcg
     1261 ataaagcgaa agagataaac caagtagatg tttgaagcaa aatacatcaa taaagaagca
     1321 atcgaaaaca gaaggaccgt ttgagaaaat actttcacac gtccatattt atcagctatg
     1381 atgccaaaaa aaatacctcc aatgagcatc cccaaattag tgatcgttga aatgaaaccc
     1441 gcttgcgtac cactaatatg aaaagaagta atcattgaag ataatgaaaa agctaaaaac
     1501 ataatatcca tattctctaa agcaattccc gccgatgtcg ataataatac ctttcgctga
     1561 tacgttgtca tttttatacc ccttttccta aaatttccct aaattgttaa aagtatatcc
     1621 aaaaaagaat aaaaaaacaa gactttttta tttgaaataa tacacaaaca accgatatag
     1681 ataatccagc ttagttttaa tagaaatgag tattgacttg gagtacactt aaagttgtag
     1741 aataaataaa atacataagt aaagtggcaa gaaattaaga gaaagagtga tcagatgaat
     1801 atcaaacaag tcagtgaaga aaagggcatt tcggccgata cattgcgcta ttatgaacga
     1861 atcgggttga ttccaccagt taatcggaca aaaggcggaa tccgtgatta tacagaagaa
     1921 gacctaaaat gggtcgactt tacgatctgt atgcgaaatg ccggattatc gatcgagtct
     1981 ttgattgaat atattcgcct atctagcgaa ggagaagcaa ctgtatttga gcggcgtaag
     2041 cttttgattg aagaaagtga acttttgacg aaagaaattg ctgaaatgca agagtgtttg
     2101 gaacgtttac aaggaaaaat caagaaatat gatcgtgtgt tatctagaaa taaaatggaa
     2161 tgtatcaagt aaaaaaatca aggagcatcg ctatggagtt acgcttacta aaatattttt
     2221 ggacaattgc agaagaagga acaatctctc aagccgcaga agttctacat cttacacaac
     2281 caaccttaag ccgacaactg aaggagttag aagaggaact aggaacagaa ctgtttgtac
     2341 gcgaaaaccg caaaatgatc ttgacagaag caggttattt tctaaaaaag ccgagcagaa
     2401 gaaattttag atttaacgaa tcaaaccact caagaattcg agaacagaaa aaaacaatta
     2461 ttcagcggcc gatttacgat cggctgtgtg gaagctgata attcgaatac attggcacta
     2521 ttactagaaa agttgatccg tgattatccc agcgttcaat ttcatatcca cagcggaaca
     2581 agtgatgata tcacggagtg actagaaaaa agcctgttag atttagcgat tttgctagat
     2641 ccagtagata cagagaaata cgaaacgatt ttgttaccaa gaaaggaaaa atggggattg
     2701 ctcattttag aaaattctgt tttagcaaaa aaagacgtga taacaaaaga agactttgcg
     2761 aatgtacctc ttttgatctc aaagcgtcca gaaatacagc gaatgtttgc tgactggtta
     2821 ggcataaaaa aggagcagtt gctgatcaca ggaacgtaca atttgatttt caatgtattt
     2881 gcgctaattg agaatcaggt aggtgctgct ttagtcatag aaggtgctgt aagtcatctt
     2941 tcaaagcgag tgacattcgt tccgcttgac ccaatgattg aaacgaactg catgctagtc
     3001 tggaaaaaga atcggatttt atcacctgta gtcaatgaat tgattcaccg tttcaaagaa
     3061 gcttttgaac agtaataagt atctctaatc gaagaaattt caatacaaaa gcgagacgta
     3121 tgacagtctt gcttttgtat tttttcattt ttaaatcaaa aaaaataaag agatttacat
     3181 a
//
LOCUS       DAHHZJ010000206         3176 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.210, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000206 DAHHZJ010000000
VERSION     DAHHZJ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.210"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            75..503
                     /locus_tag="NBL02_002670"
     CDS             75..503
                     /locus_tag="NBL02_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCD2573302.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
     gene            490..732
                     /locus_tag="NBL02_002671"
     CDS             490..732
                     /locus_tag="NBL02_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD2573303.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(1032..1247)
                     /locus_tag="NBL02_002672"
     CDS             complement(1032..1247)
                     /locus_tag="NBL02_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573304.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            1354..1668
                     /locus_tag="NBL02_002673"
     CDS             1354..1668
                     /locus_tag="NBL02_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD2573305.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1772..2950
                     /locus_tag="NBL02_002674"
     CDS             1772..2950
                     /locus_tag="NBL02_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCD2573306.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
BASE COUNT         1046 a          483 c          637 g         1010 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga aagtatttgg
      481 gtgtcaaata tgaagcaaaa taaacgatat ataccattca taccactaac gacaatcaaa
      541 gccgcagctt ctggtgattc aattgccatg tccatcgttc tgtcaaaata tcaaaattat
      601 atttcacggt tagccatgaa accagtttat gaagaagaaa aaggaatggt tatgtatgta
      661 gatgaatata tgcgtagaca actggaaaca aaattgattg agaagatact tgaatttgat
      721 acaactcgtt aaagtatttt ttccctttcc ttttcattct atggaagttt tttatcgggt
      781 caaaataaag gatttccata ggctgaaaat agcctagcgc tctttgacaa atgaatagca
      841 aatctatcag tacatgtaga ttttgattct ctagttacgc catgacaagg ttagctttta
      901 agcgacaaat tttaatatga gaatcgcgac tacgataatg atacttccgt aaccaaatcg
      961 gcccgccatt actgggggga ggtgagattc ctatgtgctt tggcaaaaca gctgatagat
     1021 cagaaaaact actatttcca tatttcgatt ttggttggca tatttttcat gccaaattct
     1081 gtgattttaa tagcatcggc aagatttaat ttttcttcag ctctctctcc acttttaagt
     1141 ttttttactg tactcaagtt gattcctgtt ccgaatgcta ttgcattagc cgtagatttt
     1201 tcaagaattt ctttaattat ttttatgtct gcaattcctt gtttcatatt aaaaattcct
     1261 cttttctagt ccgttttgat ggagtactgg agtgtttttt tacaatttta aggggtaccg
     1321 tggtggtcgt tttgattttg agagagaagg gtgatggagt tggaagttaa aacaaatgtt
     1381 aattctctgg aaatagagtt ctattcaatt ttacaaaaaa aactggaaga ttacacggaa
     1441 aaattgcttg cagtcagtca tttacctctg gtattaacaa atactgattt aaagcgacta
     1501 tttcagataa gtgatagtac actgaaccgt ttagtaaaac ttggtgagtt tcctccctgt
     1561 tggtacggga ttcgaggaca ctattttcgt gatgatgttt tagaatggat gagaaagagt
     1621 gattcgaatg actatcgaga aaaattgcga ctacttcgat ctttataagt tacattttct
     1681 ttctaagatg cggtacaatg atgacataga tagaaagatt tccaaagggt ttctgcttgt
     1741 tgggttcctt caaaatagga ggaatcaata aatggaaaaa acagatcgat tcaagaagca
     1801 aatcagttct actggtagga tcacatggta ctttcaagca tatcgaacaa caagacgtgg
     1861 ctttaattca aaacgtgagg cgcaactcgc ttatctggaa atggagaaac aacaaaggct
     1921 taagaaaaaa attgttgcaa gcaatgacaa gtttagtgtt gtaggtgagc aatggttcca
     1981 atattaccgt tcgttaaatg aacaaaagga aataacctat gataagcgag aggaacaatt
     2041 aaaaattatt gttcgatgga ttggtgataa aaagttgtct gatttgacac cagagtgcct
     2101 tcaagaattg ctttttacct taaaggaatg tggcgttaat ggaacaaatg ttggctatgc
     2161 caaaaatagt ttacaatcac ttattcaagt tctaaatatg atttttaaat attgtcaaag
     2221 aaagaatttg ataagtgaaa atccaatgaa gtctgtgaga atgccaaaat atcagttaac
     2281 ggtaactgat ttaaaagaag ccataaataa tattgaggac aagtttctta ctgtcgatga
     2341 attgagaact tttttgaatt atggcattgt tcatgaggaa ttaccgatgt ctgtactttt
     2401 ccatgttcta ttttacactg gctgccgagt cagcgaggcg cttgctctac agcctggaga
     2461 tatagatttc aaaagaaatg aaattctgtt ctataagcaa acggcagtta aaggtaagag
     2521 taaggatttt cgaattgaaa ctactaagac agttagttct gctagacgag ttctagtaac
     2581 tcctctagtt atggaaaagc tacaacagtt gattaaggct ttgggtgaga tgaggcaaca
     2641 ttacaggttt attatagaag aaacatattt gtttgtgtat cttgattcta gtaaacgtgg
     2701 tgttccttat cgtcgagagt atgtaaatga tcacgtcaaa cggtgcgtga atcgctcagg
     2761 aattaataag ccatttcata ctcatttagc tcgtcataca atggcaagct tagtagcgga
     2821 acactgtagc tgggaagtct tgaaagatcg cttaggccat actgaccaaa caacttcgaa
     2881 aatttatcgc cacttaacgt caaatgaaaa ggtaaaacct ctgattgctt ttagttcttt
     2941 agagagttaa aaaaacggtg aaagcactgg tattacagga ttcttgaaaa acgatttttt
     3001 aacaaattag tcaatatgta gtcagaggta ataaaaatcc ttttatatca atactttaac
     3061 gacttttcac aagtatcaaa gaaatccgtc gttaatcatt gttatcagac tgagaaagaa
     3121 cccttggtat tcaagggttc ttttttctta gcatgtaatt tgaacaccct gatcca
//
LOCUS       DAHHZJ010000207         3173 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.211, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000207 DAHHZJ010000000
VERSION     DAHHZJ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.211"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..208
                     /locus_tag="NBL02_002675"
     CDS             <1..208
                     /locus_tag="NBL02_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573307.1"
                     /translation="KAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRDYQ
                     LINESKYRMLVKFCDISAPAELVS"
     gene            complement(486..2285)
                     /locus_tag="NBL02_002676"
     CDS             complement(486..2285)
                     /locus_tag="NBL02_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCD2573308.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2409..3005)
                     /locus_tag="NBL02_002677"
     CDS             complement(2409..3005)
                     /locus_tag="NBL02_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCD2573309.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1124 a          590 c          565 g          894 t
ORIGIN      
        1 aaaagcagaa gtaggaagat cttatatatg caaaccgatc ggagtatctc atgaactaat
       61 cggctgtatt gaacgtacat atatcaacac ggctgtcgtg tatgttgaaa gttatgacca
      121 acgagactat caacttatca atgaaagcaa gtatcgcatg ctcgtaaaat tctgtgatat
      181 tagcgcacct gctgaattgg tttcttaaac agtatttttg agaattcgca agaactccct
      241 tataatataa ataaagacag aagggagcga gaagatatgc caaaaagaaa aagttcaggg
      301 aaaatgggaa ttattcttgc agtgatctct gcaattcctg ctattttagc tattgttcgt
      361 gcgatgaagg aatcaagaga aagttaagca cacattatga gcgtcaaaca aaaaacgcat
      421 atacctgcgg tggcaggtta tgcgtttttt gttgtataaa gacttttttc attaacgatt
      481 tttgattagc cgactcccaa aacaaaattt agcagtttat ctgaataagt gatttcttca
      541 tctgtttgtt tcacactttc atttagtagt cccatctcat cggttatgat accatccaca
      601 ccataaaaca tcatgcgact catagcatct gaatcgttga ttgtccatac atatacttct
      661 tttccgtctt ggtgggcaga attaatgaag ttacggttga tcgtactata ttccattgtc
      721 agaaaatcag caggcgtcac agggggccca acaatattga aaggtaagat gtagcccaca
      781 taaaagtttg gttctgcttc ttttaaatct gaaacggttt ggtacgtcaa actttgcaaa
      841 atatgatggt tctctaagat aactttgcgg tatttatgga caaatcgttc gacaaggtca
      901 tccgaatctt ttttagaggt cttgatctca ataagtaatt tttggtttag cttatgtgct
      961 ttttccaaat aagaatcaaa agaacaaaca ggggcttgca ctccgttttc agtaactgta
     1021 agatttgtca attctttcaa tgtaagatcc tgtggtgttc catttacccc tgttaagtga
     1081 cgaagatttg catcatgcat cactacgaaa tgaccatctt ttgtttcctg aacatccatc
     1141 tctacataat caggatgata gtctctgctt gttttttcaa gtgcttctaa agtattttgc
     1201 gccccatttt gggtactcac acctctgtga gagacagtca ctggttttgt ctgaacagct
     1261 gaagtcagat aatccatatt gtaaagacag acgccaatac cgaaaaaaac tgcaaaaaga
     1321 gtcaaaccag tgtttttcag cgcagagaaa cgcaagttgg gggcttgtgg gataaaccac
     1381 ttagggatct caggcaagaa gccttcgtca tccatgaaat caacaatgat ataaaaaatc
     1441 cctacggtag acatcacgat attgaataat aaaatccctt gtaaaagcgt catagcaaac
     1501 acagcgctga tcaatgcata gtctgggaaa aattgttcaa ccatactttg tgccaaaata
     1561 acaacgatat atcctaaaga agaaaggagt aaaatcgtac cgccaatcac aataaattgg
     1621 cccgtgatgg ccaaaagacg cgatttggtc aatgaccagc tttcccgaat ggctgctcga
     1681 aaaggacgat cccgtagaat catttccggt aaagcaaaaa tcagtcgaat cccaatatag
     1741 ccaaggaaaa tataaacgag taaaaaggaa ctgacgatga tccaacgatt agcaaaaata
     1801 aaatccataa tgaaagcggg gatttttatt tttgacagta gatcagaatt aaaacttaat
     1861 ccgctgatcg gtagaatcaa taaaaaataa gcaaggaaaa acaaaaaagt aatgggtctc
     1921 actttcttca gctgaagtat cgtcaagtgt aagagttgct tcaaagagat tggttctttc
     1981 tttttaataa aaaaaacact catcaaaaga aaagtaaatt cgaaataaac taacagtaaa
     2041 atcgtcatca aaactacgat taacgagagt agaaccccag gatgttgaga aaaaattgaa
     2101 ggaatattgt cataagacaa atagtcaatt gcccctcggt gtaaaataaa acgggtcgta
     2161 ctgctcaata agggtaaaca aataaataaa ataaagccat gcatcagcaa gacatcacgg
     2221 aaataagctt gtgttccttt taaaaagtcc caagcattac ctaagctacg tttcaaataa
     2281 ttcatcctat cacctgctct tcacattgta gcgcaaatct aatgaaaaag gaaagggtgc
     2341 agtgtgtttt gcagaccctt tcccagattt aacattgtca atcaatgact aattttacaa
     2401 aataaatatt atccaagaag accacttaga tattgacctc cataaatcaa ggccatacta
     2461 tataagaaaa tcgacagtgg tttagcaatc aaaatgatta gtgtatatcg tttcactgac
     2521 atgtttgtca atccagccat caaacataac gcatcgtcag gagcaactgg aagaaaaata
     2581 gctagagcaa acagcttctc aaaacggttt tgattatcca gccagccaat atatttgtta
     2641 tatgtcttat cgctaatcat ggaaagtatg aatggctttc cataacgacg ccctagcaga
     2701 aaaatgataa tcgaaccaat gcagattcca atatagttat agataaatcc agcataaggg
     2761 ccaaatatca ggacaccagc tgcagtactg atccctccag gaatgattgg aataactact
     2821 tgcaggattt ggatgaagac aaaaataatc ggtcctaaaa tgatggaatc acctactaat
     2881 cctcttaacg cattcaaatc tttgaaaaca cctaaattaa taaaataaat cgtcagcgcg
     2941 attgataggc ctaatccgat aatggatatg aaatttatca gttttcgaga ggcagcaata
     3001 ttcattattc atcaactcct tagaggttct ttgttaacta tactttacta gaaaagcaac
     3061 taactgcgcc attttttgat atgacagaaa acaaacaatt ttgtaagttc atcataagta
     3121 taaaagattg atctcaaaaa aacatccaac taaagactta tttaataact tgt
//
LOCUS       DAHHZJ010000208         3160 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.212, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000208 DAHHZJ010000000
VERSION     DAHHZJ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.212"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            278..787
                     /locus_tag="NBL02_002678"
     CDS             278..787
                     /locus_tag="NBL02_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCD2573310.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="NBL02_002679"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="NBL02_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCD2573311.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..>3160
                     /locus_tag="NBL02_002680"
     CDS             2322..>3160
                     /locus_tag="NBL02_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCD2573312.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIY"
BASE COUNT         1115 a          508 c          631 g          906 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga cccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa
//
LOCUS       DAHHZJ010000209         3128 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.213, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000209 DAHHZJ010000000
VERSION     DAHHZJ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.213"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(<1..115)
                     /locus_tag="NBL02_002681"
     CDS             complement(<1..115)
                     /locus_tag="NBL02_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HCD2573313.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASL"
     gene            273..536
                     /locus_tag="NBL02_002682"
     CDS             273..536
                     /locus_tag="NBL02_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573314.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            560..748
                     /locus_tag="NBL02_002683"
     CDS             560..748
                     /locus_tag="NBL02_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573315.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(831..1016)
                     /locus_tag="NBL02_002684"
     CDS             complement(831..1016)
                     /locus_tag="NBL02_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573316.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            1231..1602
                     /locus_tag="NBL02_002685"
     CDS             1231..1602
                     /locus_tag="NBL02_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCD2573317.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(1752..1824)
                     /locus_tag="NBL02_002686"
     tRNA            complement(1752..1824)
                     /locus_tag="NBL02_002686"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1789..1791),aa:Gln,seq:ctg)
     gene            complement(1870..2298)
                     /locus_tag="NBL02_002687"
     CDS             complement(1870..2298)
                     /locus_tag="NBL02_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573318.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(2358..3116)
                     /locus_tag="NBL02_002688"
     CDS             complement(2358..3116)
                     /locus_tag="NBL02_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HCD2573319.1"
                     /translation="MKKKTFKKLLFLGGTTALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1033 a          505 c          594 g          996 t
ORIGIN      
        1 gtaaagaggc cagtaaaact gaaaacttct tttttgtcca ttttatgtaa tagaaaagaa
       61 tgcttatcat aataaaaaaa ggaatataaa aaacaaattg accgatcgcg atcatactat
      121 tgcctccgat aaataaagtc catgatacaa aataacactt tgtgcgaaat ggcgcaagca
      181 gttttgtgga tttatccggt aaaatctgta tcgattttga aattagagga gaaacttgct
      241 ataatcaaag tgaaagaagg agtgggattt tcatggatca tgtagtgtta gaaatcgatt
      301 tgcatttgat caataatatt cgggtgattt actatgttgt aagtaacagt gtcgaacaac
      361 gtgtactgac gaataaaata aatggtattt tagcaaagaa agacgttcac cgattcaata
      421 atggtgaagg aagttattat agtatcccag tagaaaaaat tatttatacg acagtcaaag
      481 tcagagaaga tctagatacc aaaaaagcat atgaacctat ttttacaact tattgattga
      541 cagcagatgg aggaaaaaaa tgaatccata tgaacaactt attcaagatt tgatcgatgg
      601 aaaaatcgaa aagattgatg taaaaagaga agaattaatg gcttttcgag aagcgtggct
      661 gaagttggag aatcgtaaat atgtggtggg aaaagcctca ctgaacggca atgtgactta
      721 tcattacgat ccgacacgtt tattttagta tgaagtattc agaaaagaac caaaggagga
      781 cgatgcagca gcattgtcct cctttgattt tacaaaagtg aaagttttac ttatttgaat
      841 agatttttaa atttgttttt gacactgtcg atatttacat cttttaaaag attttgggct
      901 ttctctttat aaggtcctaa ttgttcttta tgttcttcga taaatcgttt tgcgccatcg
      961 aaattgccgt tcttaatcat ttccttgact ttttgaaata attcttcttg attcattttg
     1021 aacacatcct ttgctatcaa catgattttt tacatggaca aaacgagaaa aactgattta
     1081 tccacacgtc aataatatca aataacgatc atttttacta aaaaaacact cgattctcgc
     1141 ttctaaagta ggagaaaaat ctccaacttg ggacggatgg aatttcttta actgacccat
     1201 atactgaata tataacaagg aggtagaaag atgaaagtga ttaaatattt gattggactg
     1261 gcggcgatta gtggtatttt gctatttgga ctgaaatatt atactgaagg aagttatgga
     1321 gaaatccctg gtatacttga tcagctaaat ccattagttg aaaaaggaga ggtctatgta
     1381 aagacacaaa aaccagagga agtaaatgaa tatggtacag caaggtatgt gcagaaagct
     1441 gctgatgcca atgggaaaga acgaacggtt gaatttaatg gtctttctgt gttaaaagaa
     1501 aatcattatt tgaaaattag caataaagga gcacacatag aaacgtatga ggaagttcct
     1561 agagaaaaag ttccggagaa agcattagca attattgggt aaatcagttt ttgtattaag
     1621 aatacgcccg attttattat tcataattta attatgtaaa ctaaatacaa gtagtaaaaa
     1681 taatacaata atccccctgc aaaaagattg ttcttttaca gggggttcat tttttcagca
     1741 agcacatcaa attgggatgg cagggttcga acctacaatt gcttgattca gagtcaagtg
     1801 ccttaccagt ttggctacat cccatttatt caacaaaata atcatagcac tttcctaaaa
     1861 aaacgcaagt tatttttctt gatcggatag ttgttgcttt actcgtaaaa ggcggcgtac
     1921 tagtttcttt tctaatccag caggaagggc aatcagttct gctactgaca agacggtgtc
     1981 caataactga tattctttga caggttcatt cataatatga tagatgtttg ctaaacgttc
     2041 aatatattca aagcgagtat actctgttcg agcaattaga aatttcaata aatcggtggc
     2101 tttctcgtac tcttgatctg cgtaataaac aagagattga cgatacaatt tgatttccag
     2161 ttcattgagg gtagcaggtc tgcgacgctc gttccactca ttaatagaga ctttaccttg
     2221 aacatttccc ttgcagtcac ggacaaaaat aatcggaatt tcatttggat ccattttttt
     2281 atgtgtgaat ttttccatta tctacgctcc cttcaaaggt ttggtatctt gagtataagg
     2341 gatttttgtt tgaattacta gcttttctcc aaagttagca tctgtccacc cattgcagtg
     2401 aaaatcgccg aaaaatcgtt gatcggtact gctcgatttt ttacaccata gggatcgttg
     2461 acataaatat tttctttgtc atatccagtg actacacaag catgaacaag aggagtgact
     2521 cttacttctc cattatttgt ggtccaagtc ataaaatcag tttcatctgg gacttcaaaa
     2581 ttgatagtca cagaagtcca gacaggattt cctttagata gtgctttttt gatatcatcg
     2641 agttgagcat cggtttttgg aaaagtgtga taatccgttt ggacgatttg ctgagcaagg
     2701 gaggcaactg ggggaacata gacgaccata gcccagtctc cgccactgat atcaccaaca
     2761 aatccttcat taggatcgcc atgagtccca tctgcattga aaacagggac gtaatccaat
     2821 tgctcagcta actggttttt agttgtttca taaccataaa aagataaaag catagaaagc
     2881 gaagtgactt cacagccatt ttctaaagaa ggagaatcaa actggctctc taatggaaca
     2941 tctagtaaaa cttctgcttt ctcttttgtc tttttcgttt tttgtgttga acttttttgt
     3001 gcagtgttca ttgtatgttc tggagaatgg atatgtaggt aataacttgt accgcttaat
     3061 aaaagagcag ttgtgccccc tagaaacaat agttttttga acgttttttt cttcatttga
     3121 aatctccc
//
LOCUS       DAHHZJ010000210         3073 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.214, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000210 DAHHZJ010000000
VERSION     DAHHZJ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.214"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(<1..221)
                     /locus_tag="NBL02_002689"
     CDS             complement(<1..221)
                     /locus_tag="NBL02_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573320.1"
                     /translation="MSVDKKATMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITG"
     gene            complement(218..379)
                     /locus_tag="NBL02_002690"
     CDS             complement(218..379)
                     /locus_tag="NBL02_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCD2573321.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            complement(394..753)
                     /locus_tag="NBL02_002691"
     CDS             complement(394..753)
                     /locus_tag="NBL02_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HCD2573322.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQA
                     PLILAKKYRNTFKEERF"
     gene            complement(750..1601)
                     /locus_tag="NBL02_002692"
     CDS             complement(750..1601)
                     /locus_tag="NBL02_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCD2573323.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNTELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            complement(1618..2448)
                     /locus_tag="NBL02_002693"
     CDS             complement(1618..2448)
                     /locus_tag="NBL02_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HCD2573324.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            complement(2451..3053)
                     /locus_tag="NBL02_002694"
     CDS             complement(2451..3053)
                     /locus_tag="NBL02_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573325.1"
                     /translation="MNIDRLKTMYDGYDGDRVIEIRFIDPRRFTVQQRNFIYALIGDI
                     FIDTGMPTDFWKEFFYFRFEGVTGRKISLKDESNTTVSDANILANIILDFIFEHHIPF
                     KEGYEILPANQEYYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAA
                     LCRIHHTEKHKIGVINFKNKYQIKGIKLNQETIKKLRIGG"
BASE COUNT          742 a          670 c          486 g         1175 t
ORIGIN      
        1 aatccagtaa tttcagataa ctgttcagct gtacctgtta ctagaattcg atcaccatgc
       61 cagattgcaa tttttctcgg cgttttccgt ttaggctttt cagtccacat tgatttaccg
      121 agcttttgga cttctgcaac tatttctttg tcttcctgcc aagattctga cttggttaat
      181 tcagcaattc gtttcattgt cgctttctta tccacgctca ttcctccaat ctacgaattt
      241 cccttcttaa gttctctatg tgcaaatcga ttgccttcct cgccgtttca ttgaccatca
      301 ctgcctttgt tcgttccaga tcgtcaatct cacgctgaag gcttcgaata cgcatttgaa
      361 tcacttcttc tgttgtcatg atggaccacc tcgctaaaaa cgctcttcct tgaacgtatt
      421 ccgatatttt ttagctaata tcaacggcgc ttgatattga tgacagaaca actttgcctt
      481 gatcttaaag tcttttgtct gcattccttt gacatctacg actttgacaa gtttgccgtt
      541 tttataaaat gtgaagtcgg gaatatactc gatcttgcga tacttctttc cgtctagttc
      601 aaatttcggc atcagctcaa atctttcctg aagttttact ttccagccat ttgcttcagc
      661 ttgccacaag gctagatcgt aatactctgc ttctgcgata gaatcaaact tgatacctcg
      721 atgaacagtt tttttattac ggtatttatt catgcgatac tacctttcac tggttttatg
      781 cgcttgtctg ctgtttgttg gaatttcagc gcataacctt ctgaattctt aaatatccta
      841 gaaacaattc tttcaccgta ggcttctctt agttcaggac cagataagtt tgttgtgatg
      901 atcgttgcct tgttctgtct ggcttctaag agcgtgttta acgtgttgtt tgtaaactgc
      961 ctactatttg ataccccact acctaattca gctccaatat cgtcaaaaac caccaaatca
     1021 gttgttttaa tatcggctat aagcgatcct tcaatttctt ttctcagttc agtattgtta
     1081 taagaaaact ttatttgctc taataattct tgatagctta taaaaagtat tttcttgtca
     1141 taatttgagc gctcaagtat ttcccaagct gtggccattg ataagtggct ttttccgctt
     1201 cctgatttcc ctgatagaat gaaatgtgca ggatggttca gcagaacctc attcgtatag
     1261 ctttttgctt tttctaaagc gatttttgtt tcttgatcca ctacgtgata attttccatt
     1321 ttgcatttaa acaaagtttt atctgttaat accgaaccat tttgaaaaaa actcaacgct
     1381 cgtgctttta agctatcgtt atatatccgt tcggtctgta tatcctcttt cacacgtaac
     1441 gctttataac cacaactcat gcatgttggt ttacaacgtt ctgaaccatc cttattttta
     1501 gctcgccaac tatacaaagg ttcgctacat tctggacatt ttccgctttg cactaatact
     1561 cttcttatta gtttctccat agcatttgct aggctttcca tgtgatgcat ctccttttta
     1621 aattggcaag tcgtcatatt cactaggatt gctgtactgt agtttttgac tttgcttttt
     1681 atgattcttc ttgtctgctt tgatttcgaa tttgagcttc tcaaattttt ctctcaattt
     1741 cttagcactt ctaatatttc caaaccaaaa ttcatttgta ggtagccaat tgatcacata
     1801 ctcaatcgct tctatagatg ctttatctct ttcttccatc aacctgattg tgtctgccca
     1861 tttttcgata tctactttgt tcatttcttt tggaaaatct tcagttaaat tactttgcaa
     1921 ctttttagca aggcgtaagt gttcgtcaga atacttacct ttcttttctt ctttatctat
     1981 atctttatct tcttctatat ctttatctgt accgtcacgt gacgtcacgc taacgtcatt
     2041 ttccaatttg agacgttcct gtctctttct ttccctgtat ttacggtttc tttcagcatt
     2101 ttttagcctt actttatcca taccctcgat attttgatgt ttttcccaat tactgatggc
     2161 aattagtcca tcactgctta gatcaatcat gttgaaattt gccaatgtag ttagcgctaa
     2221 gcgaaccgta tttacgtttt tgccaaacaa tgtagcaagc atttcttcgg tataaggcat
     2281 gttcctctgg atatatatca gaccatcgtc gttagtcttt cctgctaaaa ctagtaatcg
     2341 aatccatata acgatgatgg catccgactc aggaacagct tggattaatc gtattttttc
     2401 atcgtcaaac atagtagttt taagtttgat ccaacttatc tcagccaaat ttatcctcct
     2461 atccttaact ttttaattgt ttcctggttt aacttgatcc ctttgatttg atatttattt
     2521 ttgaaattga tcacacctat tttgtgtttc tctgtgtgat ggattctgca gagtgctgca
     2581 aatgtgtact ctgaatgatc aacttctttg cgctttcgtc ttcccagcgc tttgtcaaag
     2641 tgatcgatat cagctcctgt tttgccacag atgcagcaga ctctttttgt aatgcatttg
     2701 tagaagtaat actcttgatt cgctggtaaa atctcatatc cttctttgaa aggaatatga
     2761 tgttcaaaga tgaaatctag gatgatattc gctaagatat tggcatcact cacggttgta
     2821 ttcgattcgt ctttcaggct tattttgcgc cctgtgacac cttcaaaacg aaagtagaag
     2881 aattccttcc agaagtccgt tggcatgcct gtatcgataa aaatatcgcc tatcagcgca
     2941 tagatgaagt ttcgttgctg tacggtgaaa cgtctaggat caataaaacg aatttcaatg
     3001 actcgatcac catcgtagcc gtcatacatc gtctttagtc gatcaatgtt cacttcctca
     3061 ttgatggttg cgc
//
LOCUS       DAHHZJ010000211         3059 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.215, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000211 DAHHZJ010000000
VERSION     DAHHZJ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.215"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            16..>3059
                     /locus_tag="NBL02_002695"
     CDS             16..>3059
                     /locus_tag="NBL02_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297336.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HCD2573326.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVSLPE
                     DKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDIIENPGENIPAGYHKVT
                     FTAGEGTSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPITADDTEF
                     VSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVS
                     AAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT         1139 a          485 c          645 g          790 t
ORIGIN      
        1 gagttgattt gaataatggt tagcaagaat aataagagag tatttctaga aaaaacaaag
       61 aaacgagtat tgaagtacag tatcaaaaaa ttaagtgttg gggttgcatc tgttttgatt
      121 ggtgttggtc ttgtcttggg aacaactgaa ctagttaaag cacaagatga aatttctcca
      181 agcagcccac ttgaaacagc gataagctcc gtacaagtag gtgacaaagt agcttcgggg
      241 aatacttttc aggagaatcc tggttatacg aaaaattata acttttcgga tttacaattt
      301 agtcctcaag aactaactgg agacacacta aaaggcaata caattggatt tgaagtttac
      361 ggaaaacata atattgcagc atcaactaaa aactgggaaa tccgtcttca attagatgaa
      421 cgattagcta aatatgtaga aaaaattcag gtcgatccaa agaaaggaat tggatcttct
      481 agacggacct ttgtaagaat taatgattcg ctgggtagac ctacaaatat ttggaaggtc
      541 aattatattc gtgcaagtga tggattattt gctggggcag aaacaaccga tacgcaaacc
      601 gctcctaatg gggtaattac gtttgaaaag agcctagatg aaatttttaa agagataggc
      661 atagacaatc tcaagaccga tcgtttaatg tatcgaatct acttggtaag tcatcaagac
      721 gatgataaaa ttgtaccggg tattgatagt acgggctact ttttaacaga ttcagatgat
      781 ttctataata gcttagatgt atctgaaaat aatcctgacc agtttaaaca tggatcagta
      841 agtgcaaaat atgagaaacc taatactcaa acaaaagatg gttcaggatc cacaggtgct
      901 aatggagcta taatcttaga tcataagtta acgaaaaatt ataatttctc atattcatct
      961 tccgcaaagg gcacgccttg gtatgctaat tacaaaattg atgaacgatt agttccatat
     1021 gtagcgggta tacagatgca catggttcag gctgacaaag taacatatga tgtttcgttt
     1081 gaatccggaa caaaagtagc tgatttagca atcgagcgac gggaaggtca tgaaaattat
     1141 ggcataggtt caataactaa taatgattta actaaactta tcgattttgc taatgcaagt
     1201 ccacgtccag tcgttattcg atatgtttta caattgacta agccattaga tgaaatctta
     1261 gaagatatga aagcaacagc acaagttgaa gaaaataaac catttggtga agatttcatc
     1321 tttgattctt ggttgtcgga tacgaataaa aaattaatcc agaacactta tggaacaggt
     1381 tattattatt tgcaagatat tgatggtgat ggaaaccctg acgataaaga agagagcgga
     1441 gacacgaatc catatatcgg gaaacctgaa ttagaagaag tatatgatgt tgacacaaca
     1501 gttaagggga aagtattcat ccacgagtta gcgggaacag gtcacaaagc ccaacttgtt
     1561 gataaagaag gtactgtatt agcagaaaaa actatcgctc caaatgaaaa agatggggct
     1621 ccaatttcag atactgtaga atttgaattt acgggtgtag attcaagtaa attaatcgcg
     1681 aaagatgaat taaaaatcca aatcgtttct ccaggttttg ataaaccaga agaaggttca
     1741 accgttatta aggaatcacc aaaagcggtt gataaacaaa ccgtggtagt tggatttaaa
     1801 ccagatgcta aagaatcaat tcggaataat aaaaacttac ctgaagatgc agagtattca
     1861 tggaaaacag agcctgatac ttctaacgtt actgatagta cgaaaggtat tgtaactgtt
     1921 aagatcggaa atcgaacttt cgacgtggat gtagagtttg ctgtaaaagc ttctcaagct
     1981 atggaaaatg atgcaacata cgtacctata acaacaactc cagaaacgac agttcaaagt
     2041 ggaaaaccta catttgataa accagatgtt cctttagcta aagatgcctt ttcaatttca
     2101 gatgtctata ataagtactt cggtaatgca agtgttgatg caaacactgg tattgttaca
     2161 ttcactccag ctaaaggtgt aggagaatcg gagccgatta ctggaacaat tcctattaaa
     2221 attgtttacc aagatggttc tgtaggcacg accgatttag cagtaactgt aagtaaagat
     2281 atttatgaaa atccaggaga aaacattcct gcaggctacc acaaagtaac cttcaccgca
     2341 ggggaaggaa caagtattga aagtggaaca acagtctttg cagtgaaaga cggcgtaagc
     2401 ttgccagaag ataaacttcc agtgttgaaa gcaaaagatg gttatacaga tgcgaaatgg
     2461 ccagaagaag caactcaacc aattacagca gatgatacag aatttgtatc aagtgcaaca
     2521 aaactggatg atatcattga aaatccagga gaaaacattc ctgcaggcta ccacaaagta
     2581 accttcaccg caggagaagg aacaagtatt gaaagtggaa caacagtctt tgcagtgaaa
     2641 gacggcgtaa gcttaccaga agataaactt ccagtgttga aagcaaaaga tggttataca
     2701 gatgcgaaat ggccagagga agcaactcaa ccaattacag cagatgatac agaatttgta
     2761 tcaagtgcaa caaaactgga tgataaatct gacgctgaca aatataatcc tgaaggtcaa
     2821 aaagtgacta cagaattgaa taaggaacca gacgcatttg agggaattaa gaacaaagaa
     2881 gatttaccaa aagatactaa gtatacttgg aaagaaaaag tagatgttag cgcagctgga
     2941 aataaaaaag ggactgttgt agtgacatat ccagatggat catctgatga agttgaagta
     3001 gatgttacag tgacagacaa tcgttctgac gccgataaat atgagccaac agtagaagg
//
LOCUS       DAHHZJ010000212         2980 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.216, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000212 DAHHZJ010000000
VERSION     DAHHZJ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.216"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            153..527
                     /locus_tag="NBL02_002696"
     CDS             153..527
                     /locus_tag="NBL02_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD2573327.1"
                     /translation="MSLVTKIKELADEKHVTIAEVERQVGISNGQIRRWDKASPKSEN
                     LKKVADYFGVTTDYLLGNNNVPKWATKEEVVELDKLLDSNVNMSYGGETLTPEQIQRV
                     KDILIATFWDIVKEDKEKGKKM"
     gene            532..960
                     /locus_tag="NBL02_002697"
     CDS             532..960
                     /locus_tag="NBL02_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCD2573328.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRDRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIEELKNLYGLPESAYRFLI"
     gene            1010..1663
                     /locus_tag="NBL02_002698"
     CDS             1010..1663
                     /locus_tag="NBL02_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573329.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDTTSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQATAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            1799..2365
                     /locus_tag="NBL02_002699"
     CDS             1799..2365
                     /locus_tag="NBL02_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HCD2573330.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWLLAVVVVFIAIGSQGGSD
                     DAKNTVAETTKESVTEVSSAESVAESTVVEEETETTETTIEEVTQEESVPREYRNALS
                     KAESYLGWAGMSEQGLREQLEFEEYPSDAIDYALANVDVDYNEQALAKAESYDDWASM
                     SDSQLYDQLIFEGFTNEQAQYALDNLPQ"
     gene            2481..>2980
                     /locus_tag="NBL02_002700"
     CDS             2481..>2980
                     /locus_tag="NBL02_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCD2573331.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLHKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTIDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PIDEVV"
BASE COUNT         1129 a          476 c          561 g          814 t
ORIGIN      
        1 gaatgtttta aattagtaga gttttttgtt gctcttattt agctaacaat ttagcttacg
       61 aattaaatat actagcttaa aagctaattg tcaaccaaaa atataacttt taagctattt
      121 attttctttt cagctttgaa aggttgataa taatgagttt agttactaag attaaagaat
      181 tagcagacga aaagcatgtg actatagcag aagtagaaag acaggtgggc atctctaatg
      241 gacaaataag aagatgggat aaagcctcgc caaaatctga gaacttaaag aaagttgctg
      301 attattttgg tgtcacaact gattatttat tgggaaataa taatgttccc aagtgggcta
      361 caaaagagga agtggttgaa cttgataaac tactagactc aaatgttaat atgtcttatg
      421 gtggggaaac attgacaccc gaacaaatac agcgcgtaaa agatatcctg atagcgactt
      481 tctgggatat tgtgaaagaa gacaaagaaa aaggcaaaaa gatgtgagct tatggagatg
      541 gatacgatta atttagtcga ggagttgaag cggaaatacc agtccgctaa tcctttttat
      601 atttgtgaaa agatgggcat taaaattcaa tacgttcctt ttatcgagaa tcccaagggg
      661 cagtttcaag aaattagaga tcgtgcgata atctttttaa atgatgaact gcgagactct
      721 gaggaaagat tctacatttg cgctcacgaa ttaggtcacg ctatttttca tcgtggctta
      781 tccagctatt atgtatcaac aagaacatct agaagcaaat ctgaaagcga agctaattgc
      841 tttgctgcta atctcattgt ttctctttat aaagaagata atgatcaata tcctaaaaaa
      901 atcgaggaat taaagaatct ttacgggctt ccagaaagcg cttatcgttt tcttatataa
      961 aaaagcccgt gttagcacac atattacaac gagaaagagg aattataaaa tgaaaaaagt
     1021 tagcgttatg ttgttgttaa gtactgctct gctactttca gcttgttcaa ataataaaaa
     1081 agctgaatca acagatacca cttctaacca agaaacaaaa ataagtaaaa caaaagaaac
     1141 aactgaaacc agttcatcta ctagcaaatc tacatctaaa acagattcta gttcaacagt
     1201 tacaagctcc aaccaagcta cggcggaacc tagcccaaca gttataagct ccagtcagag
     1261 tacaatccaa accgcacctc aagaagaaac atatgaacag atgaaacaac gcactttaca
     1321 gtcaactcca gctgatcgtg caaattggtc caacaaagag tgggaagctt tcggcgtggc
     1381 cctttatgaa aatggattga ctacagatga tgctggcaat attatcagtc aagatcagaa
     1441 agaacaacaa gcagcatctc aacagaatcc agaagaccaa caaacaagcg ctcagcaaga
     1501 cgctgacact ttatcactta ctgattttgt taacaaatac gggatgtcgc ctgttgcatg
     1561 gaaagtacag aatggaatgt ctgaagaaga agcattgcgt acaacacagc aaaagacttc
     1621 cggtgaagtt caattaggat tttctaaata cggaattcaa taatacattt ttatgcccta
     1681 ctattttgcc tataatctct aaaaaagtta taaagaaaaa agcccgtgct gcaacacgga
     1741 ctcatacctc atttctgaga tcacaaatat attataacaa gaagcgaggg atatttaaat
     1801 ggcaaaaaaa gttatgggtc aagatgggaa aatgtataaa gttaagaaac cgttttataa
     1861 acgggtatgg ttttggttgt tagcggttgt tgtggtgttt attgctatag gttcgcaagg
     1921 aggcagtgat gatgctaaaa ataccgtcgc tgaaacaact aaagaaagcg tgacagaagt
     1981 gtcttctgca gaatcggtag cagaatctac agtcgttgaa gaagaaactg aaactactga
     2041 aactactata gaagaagtta ctcaagaaga aagtgttcct cgtgaataca gaaatgcatt
     2101 gagcaaagct gaaagctatc taggttgggc tggtatgtct gaacaaggtt tgcgtgaaca
     2161 actagagttt gaagaatatc caagtgatgc aatcgattat gcgctggcta atgttgatgt
     2221 cgattacaac gaacaagctt tggctaaagc ggaaagttac gatgattggg catcaatgtc
     2281 agattcgcaa ttgtacgatc aacttatatt tgaaggtttt acaaatgagc aagcacaata
     2341 cgctttagat aacctaccac aataactaac aaaaacacgc ccctccctcg ccaaagtttg
     2401 tggacgtgat gaaaaataaa cctgctataa tcggcttact tatctattcc tattatagca
     2461 acaaatagga gatgaaaaca atgtggatcg aaactaaaac tgataaaaac ggaaaaaaag
     2521 tatataaata taatgagcga tatattgatc caaaaactag aaaaagaaaa aaagtgagca
     2581 ttacttataa aaataaatct cgagaaactc agaaagtggc attgctagag ttaaataaaa
     2641 aaattgatat aaaattaaac gaaaaaacac ttcataagcc tgatctaaca tttcatgagc
     2701 ttgttgaaga atggttagtt atatacaaaa gacaagttaa ggaatctaca tattatccta
     2761 cgaataatat attaaacact ataaagaaga agatacctga gacttacatc gtttctggta
     2821 ttaatacaat agatttgaat aatatttttg aagacatgat atataaagat gacttgtcaa
     2881 ataagtacgt cagtgtaatt aaatccaaat tgaatcttct tttttcatat gctatgaaaa
     2941 aaggttatgt agaaaaaaat cctatcgatg aagtagttat
//
LOCUS       DAHHZJ010000213         2956 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.217, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000213 DAHHZJ010000000
VERSION     DAHHZJ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.217"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(65..607)
                     /locus_tag="NBL02_002701"
     CDS             complement(65..607)
                     /locus_tag="NBL02_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HCD2573332.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            742..1131
                     /locus_tag="NBL02_002702"
     CDS             742..1131
                     /locus_tag="NBL02_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573333.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1135..1899
                     /locus_tag="NBL02_002703"
     CDS             1135..1899
                     /locus_tag="NBL02_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCD2573334.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2028..>2956
                     /locus_tag="NBL02_002704"
     CDS             2028..>2956
                     /locus_tag="NBL02_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCD2573335.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELV"
BASE COUNT          944 a          497 c          609 g          906 t
ORIGIN      
        1 taaataaaaa ctgtctaaca tttaaaattt aatttagata ttagacagtt tttaagtttg
       61 tgagttattt tccttgtcgt tttctcactt ttttagtcca gaaaccacct gaaatatatt
      121 ttggctcata agaaatgata aaagcacgct cttcgatttc tttgatcagg cgatacagtt
      181 cacgttcgct ttttctagga gaaagtattt cgagaactag ccgctcacct tccagcccca
      241 ttgcagaagt ctgtgtcaca ccataaccgt gttctctcag tatacccggc aaacttttat
      301 cttctgcgct atttgctggc aaaatcgttg tgaccatgat gtatcctaat gccaatttgt
      361 cttcaatcaa aattccgaca ctgataccta ctgcgtaacc taacgcatag acaaataaat
      421 tcagtggatt atctaaacga tctaaaacaa gggataagcc gacgatataa atcgtgatct
      481 cgaccatact gacaagtgga gcaatcagtc gatagccttt cattgtcaac atgaaacgta
      541 acgtgttcag agtgatataa gcaaaattaa tgaggaagat catccccaac atttttggat
      601 caatcatttg taaaactcct ttcatttata taacaacaat atcaaaaaaa atcgtaagtg
      661 aagaaaaatt gaaactttat acatacaaaa aaatgataaa atgattataa tagaattata
      721 aagataggtt aggtgattaa aatgaaaacg gtcaatcgtg tgttttgtgc cagccacgtg
      781 ctaagtctcc ttctcagtat atacttaggt gtctattcga ttcaacagcc aattgaggta
      841 gagaacattg tttttcagat gtcgctgaca gatggtttaa ttttaagcgt ttttttcttt
      901 ttgatactgt ttattggtaa tattttcggt agcgttgcca gttttttgaa tttttctatt
      961 tatccactcc tttcgctagc attaggagtg gtgggacttt gttctctgtg cttatttccc
     1021 gagccattca gtcctgcatt tatattattt ggtacactga acctgttcca gacaactgtt
     1081 ggagcatggt tgctatggcg atcaggaaat ttaatgaaaa taggtgagta acaattgaat
     1141 ccaattatat tgaaacgttc tgtccatcat attccagtgt tagaagttgt tctgaaagaa
     1201 ttaagagagg aacgtattcc cgttgtcatc tattatcacg gatggcagac aagtaaggag
     1261 ttagttttga cgcaaggaag aaaaattgcg cagcagggca ttcgtgtgat cctgccagat
     1321 gcgatgaatc atggggagcg caaacagccg gtttcttcta ttccatccta tacattttgg
     1381 gatagtatct acggaaatct gtttgaattt gacacactta tcaagcattt agaaaaaaga
     1441 ggtcttctac atgatcagct tgcagttggc ggggtttcga tggggggaat gacaacatgc
     1501 gccttgttgg ctaaacatcc tcatatagta gcgggcgtgt gtttgatggg ttcccctgca
     1561 cctttggaat atggaaaaga aatcgtaagg cgcgcatcgg aatataatta tgtcgtacca
     1621 aaagactatt ttgatttgat ttcatgggtt aagagctatg atttgtcatt gcagccggaa
     1681 aagttagctg gacgtcctct gttcttttgg catggacaag ctgacgaaaa aatcccttat
     1741 atacaaacgg aacgatttgt agaaaaaaat cggcataagt cttacggaaa agaaattgtt
     1801 tttcgttcct caaaaacaca gcgacatatg gtacaaatac cactaatgga agaagcttcc
     1861 acttttttag caaaaaattt gataaaaaag tataaataat tgaaacggaa aatcactcat
     1921 gacgatagag gattttccgt tttttatttt gggctataat aaactctgtt aggaaaattt
     1981 ggtataattg atagtaagaa tgacaacatg ttagaaagga agagagcatg cccactttag
     2041 cagatgttgc aaaacgtgcc aatgtttcaa aaatgacggt ctctcgagtc atcaatcatc
     2101 cggaacaggt gactgacgaa ttgaaagagt tagttttctc agcaatggct gaactggatt
     2161 accgtccaaa tattgctgca aaagcacttg tcacgaatcg gtcgcagatc atcaaattat
     2221 ttatactgga agaaatcgac acaactgagc cctattacat gaatttgctg atgggaatcg
     2281 ctaaacggat cggtaaagcg cattattctc tccaattagt tacaaatgat gccttcgacg
     2341 tcggttcttg tgatggatac atcatcacag gtgtcagaaa aaaagacttc gattggatcg
     2401 accgtttgga aaaaccagtt gtcttgtttg gggaaaattc tgaaggatat gattatgtag
     2461 acagcgataa tgaatatggt acagcaaaag caacgcaata tgccttagaa agagattatc
     2521 aaacgattat ttatatcggt atcgaagtgg acgagccttt tgaaatttca agggaaactg
     2581 gttatttatc tgtgatgaag gaagttgatc ttcctagtaa aatcttgcgg tttccaaatc
     2641 attcgacaga agtagaaaga tatatgacag aaaactgggc agattttaca gataaaactt
     2701 gttttgtatg tagttcagat cggctagcac taggagcagt aagagggatt ataaatgctg
     2761 gaggacactt gcccgaagaa tttggtgtga ttggatttga tggggtattt ttagaccaaa
     2821 tcagttcacc taaattgaca acgatcaagc aagatattat acgtatgggt gaagtatgtg
     2881 gagaaatgtt gttaaaaaaa atagaagaaa aaggggcgag tcaaggttat cgtcgttttt
     2941 ttcctgaact agttat
//
LOCUS       DAHHZJ010000214         2812 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.218, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000214 DAHHZJ010000000
VERSION     DAHHZJ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.218"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(<1..201)
                     /locus_tag="NBL02_002705"
     CDS             complement(<1..201)
                     /locus_tag="NBL02_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573336.1"
                     /translation="MEEFNMDVDALFKQTVFKTVNKEFKIDFQNDESFPTEIELFEEL
                     SQSVSESFSRQLQKNFFDALVDE"
     gene            257..1033
                     /locus_tag="NBL02_002706"
     CDS             257..1033
                     /locus_tag="NBL02_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HCD2573337.1"
                     /translation="MNTPQIFNFGQQEVRTVLLNDEPYFVGKDVAEILGYERADNAVR
                     NHVDEEDRLMHRISASGQNRNMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATKLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMRQGYTKSHTTEIPK
                     SDGGTKVVMNTKWTQKGRLFIYESLKKEGYIPEIDLLEER"
     gene            1036..1248
                     /locus_tag="NBL02_002707"
     CDS             1036..1248
                     /locus_tag="NBL02_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD2573338.1"
                     /translation="MSQDFILKVRVALATHNKSQAWLAEKLDISTAYMSDIMNGRRKP
                     DKQIKPIEAVLAELEKEKKHANNNSR"
     gene            1226..1510
                     /locus_tag="NBL02_002708"
     CDS             1226..1510
                     /locus_tag="NBL02_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573339.1"
                     /translation="MQITIPDNLVVSELTTQITNAVLNSLDERLHLMNKSVELPPYPN
                     KSEVRKVLGIGDDKLTHWINLGLKTQQWSKLDIRIERSELQRFLKENFEF"
     gene            1531..1719
                     /locus_tag="NBL02_002709"
     CDS             1531..1719
                     /locus_tag="NBL02_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573340.1"
                     /translation="MSYTLQQEHQILRLIKQRRKQLQDDREALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            2045..2386
                     /locus_tag="NBL02_002710"
     CDS             2045..2386
                     /locus_tag="NBL02_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573341.1"
                     /translation="MENELSTLDQYLTDPSWGKSNIKETNNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            2379..>2812
                     /locus_tag="NBL02_002711"
     CDS             2379..>2812
                     /locus_tag="NBL02_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HCD2573342.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGDFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIP"
BASE COUNT         1053 a          453 c          556 g          750 t
ORIGIN      
        1 ttcatccacg agagcatcaa aaaaattctt ttgcaactgg cgagaaaaag actcagaaac
       61 gctctgagaa agttcttcga ataattcaat ttctgttgga aaagattcgt cattttggaa
      121 atctattttg aattctttat taacagtctt gaatacggtt tgtttgaata aggcgtcgac
      181 atccatatta aattcttcca tactattttc acctcgcttt ctattcaaaa ttataccaaa
      241 gaaaggaatg agcaaaatga acacaccaca aatttttaat ttcggacaac aggaagtaag
      301 aacagtttta ttaaatgatg aaccgtattt tgtaggtaaa gatgtagctg agattcttgg
      361 atacgaacgt gcagacaacg ctgtcagaaa tcatgttgac gaagaggata ggctaatgca
      421 ccgaattagt gcatcaggtc aaaatcgtaa tatgacaatc atcaacgagt caggccttta
      481 cagtttaatc ttaaaatcaa aacttccttc tgccaaaaaa ttcaaacgat gggtaacaag
      541 tgaagtgttg ccagcaatta gaaaacatgg aggttatcta actccagaaa aagtagaaga
      601 agccttgctt aatccagata caatcatcca attagcaact aagttgaaag aagaacgtac
      661 tggaagacta atcgcagaac aaaaaattgc agaatacgaa cccaaaattt cgtatttgga
      721 tagtatctta tcttctacag attcagtaac aattagtcag attgcagcag attatgggat
      781 gtctccacaa cagatgaaca aactacttca taaactaggt attcagaaaa aagtaggcaa
      841 ccaatggtta ttatgcaaga aacatatgag acaaggatat acaaaatctc atacaactga
      901 gattccgaaa tctgatggtg gaacaaaagt tgtgatgaat acaaaatgga ctcaaaaagg
      961 acgtttgttc atttatgagt cgctaaaaaa agaaggatat attccagaaa ttgatctatt
     1021 agaggagaga tagttatgtc gcaggacttc attttaaaag tgcgtgtggc gctagctacg
     1081 cataacaaga gtcaagcttg gttagctgaa aagcttgata tttcaacagc ctatatgtcg
     1141 gatattatga acgggcgtag aaaaccagat aaacaaatca agccaattga ggcagtacta
     1201 gcagaattag aaaaggagaa aaagcatgca aataacaatt ccagataatt tagtagtttc
     1261 agaattaact acacaaatta cgaatgctgt gcttaattca ttggacgaac gattgcacct
     1321 tatgaacaaa tcagtagagc ttcctccata tccaaacaaa tcagaggtaa gaaaagtttt
     1381 aggaattggt gatgacaaat taacacattg gataaaccta ggcttaaaaa cacagcagtg
     1441 gagcaagtta gacatcagaa ttgaacgatc ggaactccaa agatttttga aagaaaactt
     1501 tgagttctaa aggcaaagga gaatgatttt atgtcctaca cattgcaaca agaacatcaa
     1561 attctccgtt tgattaaaca acgcaggaaa caattacaag atgatcgtga agcgcttaga
     1621 aaagccgatg agctatcaga tagacaagct gaactaatcg cttctgaact tgaggatttg
     1681 agaatgctag aaataaaaaa tagggagatt agattatgaa gaagacagac acacttttta
     1741 taggattcat cctagggcta ttagtgatag tagtacacca aagtattatc gggggaagtt
     1801 tgttcgcagc attgatggtt ttaatcaatc tgcttgattc aaaagaaagg agcaactatg
     1861 gcacgagaag aagcgctaaa aatcggtaaa gtgattgctg ataattggtg ggcaaatagc
     1921 cgtcctatta ttttaagcaa gcaacatatc gaaaagcaaa aagcatggca acaaataaaa
     1981 aagtgactcc gccgaccaaa gcaatgagtc actcaaaaaa tcataactaa ggagatttta
     2041 acatatggaa aatgaacttt ccactctaga tcaatatttg actgatccta gttggggcaa
     2101 atcgaatatc aaggaaacaa ataatcgaaa aatcagacga aatctcttga cggatgaaga
     2161 actagcatgt gatcaagatg acttgggaaa ttttgtgagt atttgggatc atgtctatct
     2221 tatccattta tcgaggaagt ccaaaaaacc tgagtatatc tatgtcatcg aagatggctt
     2281 gattgatgcg ctagaagagt acgacagaga taacttgatt gatatctctt attacggacc
     2341 aggtaagaaa tacattgctg aaatggaggc agaatttgat gagtgaaatc aaagggacaa
     2401 cgaactttga aaaacttttt agtcgtaagt taaataaaat tctcaagaaa aaaggagatt
     2461 ttgattattt atcttgggct cacgcgtggg agattatgaa aaagaatgat ccacaggcaa
     2521 cggtaactat taatgagtac aaacactaca gggttgtttc tggaactcat caagactttc
     2581 ttgttgagga atataaacct tttcttatgg atgaaactgg gacttatgta tctgtctcag
     2641 taacggttaa aggacacacg gaaaccgaat tatttcctgt tttagattat cgaaaccaac
     2701 cagttgttaa accaaatgca atgcaaatca ataactcatt gaagcgatgc tttgtgaaag
     2761 cattggctct acacggactg ggattatatg tatttcaagg ggaagatatt cc
//
LOCUS       DAHHZJ010000215         2787 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.219, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000215 DAHHZJ010000000
VERSION     DAHHZJ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.219"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            85..972
                     /locus_tag="NBL02_002712"
     CDS             85..972
                     /locus_tag="NBL02_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HCD2573343.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="NBL02_002713"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="NBL02_002713"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCD2573344.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="NBL02_002714"
     CDS             2274..2720
                     /locus_tag="NBL02_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCD2573345.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
BASE COUNT          935 a          450 c          608 g          794 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttat
//
LOCUS       DAHHZJ010000216         2701 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.220, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000216 DAHHZJ010000000
VERSION     DAHHZJ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.220"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            233..1521
                     /locus_tag="NBL02_002715"
                     /pseudo
     CDS             233..1521
                     /locus_tag="NBL02_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(1647..2684)
                     /locus_tag="NBL02_002716"
     CDS             complement(1647..2684)
                     /locus_tag="NBL02_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCD2573346.1"
                     /translation="MTVYQRFLANEKIRRLTVLLFVVFVLFLVRSMITTILLTFIFTY
                     LMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLIHQTFDMIQSVINFY
                     EHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKMAVAFAMSFILSFFF
                     MIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQFVVALLNTLLTTIA
                     LAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGIRDVVYILITILIVH
                     LIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIPIFTFFLDVLKVKKI
                     PNHLPKETDIP"
BASE COUNT         1086 a          503 c          469 g          643 t
ORIGIN      
        1 gttcagatag gaaaagaaaa atatctaatt ttgtattgct ttttttcttc aagagaaacg
       61 ttttaatcgc ttcttgagtt tgcatgaaag aggctccttt ttacttaaat tgttaaaact
      121 tgaaaatatt actgtttttt tcataaattt agacaaaaaa acagtaaaca tataggtaga
      181 atctctacta tcttatttct tgcatacaaa aaaatcaagg agtgagagag ttatgagata
      241 ttcaaccatc ttaatatcaa catttgcagc cttaagctta tcgcttacta taaatcagac
      301 agtccacaca gaggagatag aaacagccaa atggacagaa accaagtggg ccaacgtcaa
      361 tcgaattacc tttcaggatg aattttcatc cttcaattca ggagtcgcaa aattaagaaa
      421 aggacaaaca gcgaccatca aagcaaaaat tgactattca ggagacttag cacctatact
      481 acaatcagct gttacatttg agaatgttga tcccgctttg tcgattggct atgacgagaa
      541 cactttgact atccaacaca agcaagcgat atttaccttc accgttacat taaatcagga
      601 cttgttaaaa ccagcgcttt ttactgtcaa ggtatcagat ggactaccga acgatcatca
      661 tcatttaacg ccgtatagtc agcggcagac aattgagcaa gattcagcta tcgactcagg
      721 aggagatctt gttgaagaac caacagataa accagaaaat gaaaataagc cagaagtacc
      781 gcctacagaa aatccagacg gagaacagaa accggaaata gaacctggag aagaacccga
      841 tacagaaact caaccagagc cagacaatga atcaaaacct gaaataaccc ctggagaaaa
      901 accagatgta gacccagaag aaaaaccaga tgtgactcca gaaccagata cggattctgg
      961 aaaccaaaca gtaccggaaa caaatccaga cacagataat gaaactgaaa atccagaaaa
     1021 accagaggta gccccagaag aaaaaccaga tgtaactcca gaaccagata cggattctgg
     1081 aaaccaaaca gtaccggaaa caaatccaga cacagataat gaaactgaaa atccagaaaa
     1141 accagaggta gatccagaag aaaaaccaga tgtgactcca gaaccagata cggacgcaag
     1201 agaccaagga ataccagaaa aaataaacaa aaaaacaatt caagaggatg gaaaaaaaga
     1261 gagtaaaaaa tcaaatctag caattctaaa aataaatgaa gagcagctga ataaaaaatc
     1321 acgaatattt gatagcgccc aatcagctga aaccttaaaa agtagcaagg atacaacctt
     1381 tgctgtcctg aaacgaaaaa taaacagcta ccaaaatccg gagagtcaca aaacaaagtt
     1441 atactgtgga gcggaatcat tctattaagt atagcaacaa tgctttctgc aaaaagattt
     1501 aaacaaaaca gatctctcta acatttgaat aatgtctgtg aacgcatttt tcaaataaaa
     1561 agaaatatcc agaaaatgac cccaaaatac cagattgaaa tctgatattt ggggtcgttc
     1621 atactttatc tttcttcata tttattttaa gggatatctg tttcttttgg aagatggttc
     1681 gggatctttt tcactttcaa tacatctaaa aagaaggtaa agatcggaat gccgacaatc
     1741 aagccccaaa cgccaaagaa ccgttcgcta acaagtaaga ccacaaatgt ataaaaaatc
     1801 ggcagttctg ttttgctgga catcaacttc ggattcagca cataagactc aattagatgg
     1861 acaatcaaaa tcgtaatcag gatataaaca acatctcgga tgcctccttg ggaataagct
     1921 atgaaagata gaggaataca agaaatgata acacctgcta ctgggatcag acttaaaatg
     1981 aaaatcatga tcgctaaact cagcagctga tgaaagccaa atacagccaa ggcgattgta
     2041 gtgagcaacg tatttaaaag agcgacaaca aattgagctt ctaataccaa accaaatgta
     2101 ttgacaaact tatcggcaaa atagtaaata tcttgaaaga accaagaaaa ttcacttttt
     2161 aaaaacaatc ttgagaatac aattgttttt ttcttttcaa tcataaaaaa gaaacttaag
     2221 atgaatgaca tagcaaacgc cactgccatt tttcctatat cctgtaaata gccaagcgca
     2281 atcgaagcgc cattttgaag ctgttcaaaa aaatcgtttc gctcaatata agtatggata
     2341 tattgtaaaa caggatctgc atcttttggc tgatgctcgt aaaaattaat aacggactgg
     2401 atcatgtcaa atgtttgatg aataagcatc gggatatact ttgtgaatgc cagatagata
     2461 aggtaaacga tcagtgtata aatcatgata gctaaaacac ctgtagggat tcggacaaat
     2521 cgctgaatga tttgaacaag atgaatcatc aaataagtaa agataaatgt gagcaaaatg
     2581 gttgtaatca tacttctaac taagaaaaga acaaaaacga caaataataa aacagtgaga
     2641 cggcgtattt tttcattcgc caaaaaacgc tgataaacgg tcaaatcgac aacctccaaa
     2701 t
//
LOCUS       DAHHZJ010000217         2601 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.221, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000217 DAHHZJ010000000
VERSION     DAHHZJ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.221"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            21..623
                     /locus_tag="NBL02_002717"
     CDS             21..623
                     /locus_tag="NBL02_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573347.1"
                     /translation="MNIDRLKTMYDGYDGDRVIEIRFIDPRRFTVQQRNFIYALIGDI
                     FIDTGMPTDFWKEFFYFRFEGVTGRKISLKDESNTTVSDANVLANIILDFIFEHHIPF
                     KEGYEILPANQEYYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAA
                     LCRIHHTEKHKIGVINFKNKYQIKGIKLNQETIKKLRIGG"
     gene            630..1481
                     /locus_tag="NBL02_002718"
     CDS             630..1481
                     /locus_tag="NBL02_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HCD2573348.1"
                     /translation="MADNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIEVLDNGAIYMSDI
                     QSFIGKSSTEADRKRKYRKEIEEAKQNLITSGQMSDKCPDKTTPELEIELEKDIELEK
                     DIENVTPSKKSKAKPVRHKYGEYKNVLLSDEQMEKLKIEFPNDYQERIERLSEYCESS
                     GKTYKNYLATIRSWARKEKSESKNASSGYKRTGRREKLPEWAIDQEAYLKKKALERAN
                     RQSKAPF"
     gene            1493..1762
                     /locus_tag="NBL02_002719"
     CDS             1493..1762
                     /locus_tag="NBL02_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573349.1"
                     /translation="MKIDYLELINEIAKYKTGEEIEILRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1767..1931
                     /locus_tag="NBL02_002720"
     CDS             1767..1931
                     /locus_tag="NBL02_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573350.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            1924..2226
                     /locus_tag="NBL02_002721"
     CDS             1924..2226
                     /locus_tag="NBL02_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573351.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            2223..2384
                     /locus_tag="NBL02_002722"
     CDS             2223..2384
                     /locus_tag="NBL02_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCD2573352.1"
                     /translation="MTTEEVIQMRIRSIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEG"
     gene            2387..>2601
                     /locus_tag="NBL02_002723"
     CDS             2387..>2601
                     /locus_tag="NBL02_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573353.1"
                     /translation="MDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAEQLSEITGL"
BASE COUNT          989 a          423 c          581 g          608 t
ORIGIN      
        1 gtgcaaccat caatgaggaa gtgaacattg atcgactaaa gacgatgtat gacggctacg
       61 atggtgatcg agttattgaa attcgtttta ttgatcctag acgtttcaca gtacagcaac
      121 gaaacttcat ctatgcactc ataggcgata ttttcatcga tacaggcatg ccaacggact
      181 tctggaagga attcttttac ttccgctttg aaggtgtcac agggcgcaaa ataagcctca
      241 aagatgaatc gaatacgact gtgagtgatg ctaacgtctt agcaaatatc atcttagatt
      301 tcatctttga acatcatatt cctttcaaag aaggctatga gattttacca gcgaatcaag
      361 aatattactt ctacaaatgc atcacaaaaa gagtctgttg catctgtggc aaaacaggag
      421 ctgatatcga tcactttgac aaagcgctag gaagacgaaa gcgcaaagaa gttgatcatt
      481 cagagtacac atttgcagca ctctgcagaa tccatcacac ggagaaacac aagataggtg
      541 tgatcaattt caaaaataaa tatcaaatca aagggatcaa gttaaaccag gaaacaatta
      601 agaaacttag aataggagga taaaaaatat tggctgacaa caaacgatac tactatttaa
      661 aactaaaaga gaactttttc gatagcgacg agatggttct tttagaaagt atgccagatg
      721 gatacattta ttctaatatt cttctcaaac tttatttaag aagtctgaaa catgaaggca
      781 aactaatgtt taatgacagg attccattca attctacaat gcttgcgact attacaagac
      841 actctgtggg agttgtagaa aaagcagtac aaatatttcg tgatttacaa cttattgagg
      901 tattagataa cggagcaatt tatatgtctg atatacaaag ttttattggg aaatcttcaa
      961 ctgaagctga taggaagaga aaatacagaa aagaaattga ggaagcaaaa cagaatttaa
     1021 taacaagtgg acaaatgtcg gacaaatgtc cggacaaaac tacaccagag ttagagatag
     1081 agttagagaa agatatagag ttagagaaag atatagagaa tgtaacgcct tcgaaaaaat
     1141 cgaaggctaa gcccgtccgt cataaatacg gagagtataa aaatgttctt ttgtcagatg
     1201 agcagatgga gaaactcaaa atagaattcc ctaatgacta ccaagagcga atagaacggc
     1261 tatctgagta ttgtgaatca tctggtaaga cttataaaaa ctatttggca actattcgaa
     1321 gctgggcaag aaaagaaaaa agtgaatcta agaacgcaag cagtggatac aagcgcacag
     1381 gaagacgaga gaagcttcct gagtgggcaa tcgaccaaga agcctatctc aagaaaaaag
     1441 cgctagaacg agctaataga caatcaaaag caccattcta agaggtggga aattgaaaat
     1501 cgattatcta gaactaatta atgaaatagc aaagtataaa actggtgagg aaatagaaat
     1561 cctgagagac gtatatgatc aactcgaaga agctggaatc gaaggaatta agaatgatcg
     1621 ttcgagttgg agtaagctca gatactattt cgcactttat atcgatacaa cacaattaag
     1681 aaatttagca tataccaaat tactatttgt tgattgtgtt aaaggcttgc aaaaacatct
     1741 taatgaactt gagcaggtgt aatcagatgg acctaaagac atttacagca cagattgaac
     1801 taatgcatca agaagcttta agacaaagtg tatcgtacga agacaagtgg ctcaacacgt
     1861 tccacggcgg acgtgagagc gcacttgatc aagtactcaa attactgaaa ggagaatgtc
     1921 gggatggata agaaagcggc aatgcagcga attatcgaat tgacttattc agaagattgg
     1981 caaaatgaca aagaagctgc ttcagaagtg atgaggcttg gaagagcgat gtgggcagac
     2041 aagagcaaca agccaagacc acgaaaaatc gcaatttggc acggtgacaa acttctagtg
     2101 atagggacag ctgaacagtt agcaagtctc acaggcttgc acgagaaaat cgtgaggaaa
     2161 agagcaaggt gtggctacac agacgctaag aagagaacgt ttagatacgt ggaggaatca
     2221 tcatgacaac agaagaagtg attcaaatgc gaattcgaag cattcaacgt gaaattgacg
     2281 atctggaacg gacaaaggca gtgatggtca atgaaacggc gaggaaggca atcgatttgc
     2341 acatagagaa tttaagaagg gaaattcgta gattggaggg gtaaggatgg ataagaaagc
     2401 agcaatgaaa cgaatcatcg aactgacaca ttctgagaat tggcaagaag acaaagaaat
     2461 agttgcagaa gtccaaaagc tcggtaaatc aatgtggact gaaaagccta aacggagaac
     2521 gccgagaaag attgcaatct ggcatggtga ccgaattcta gtaacaggta ctgctgaaca
     2581 gttatctgaa attactggtc t
//
LOCUS       DAHHZJ010000218         2572 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.222, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000218 DAHHZJ010000000
VERSION     DAHHZJ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.222"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            20..868
                     /locus_tag="NBL02_002724"
     CDS             20..868
                     /locus_tag="NBL02_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCD2573354.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            968..1402
                     /locus_tag="NBL02_002725"
     CDS             968..1402
                     /locus_tag="NBL02_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCD2573355.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            1582..1992
                     /locus_tag="NBL02_002726"
     CDS             1582..1992
                     /locus_tag="NBL02_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCD2573356.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            2049..2489
                     /locus_tag="NBL02_002727"
     CDS             2049..2489
                     /locus_tag="NBL02_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCD2573357.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
BASE COUNT          802 a          422 c          509 g          839 t
ORIGIN      
        1 gaattaagga agtgaataaa tgaaagaaac gagacaattt atatggacat acattaagat
       61 tatttttgcc ctcttcatat taggagtcgc tatcaatatg tttttagggc ctcatcatat
      121 tgctgctgga ggtgtcagcg gtctaggaat attactggaa gctgctttag gctttgaccg
      181 agctatagtt attttagtgt tgaatatcgt tatgttggta ctagctcttc tatttttagg
      241 taaaaaaccc ttttttaaag tacttttcgg aagcttggtt tttcctttaa tcattgcaat
      301 agttccagaa acaatgatta cttcagatcg gttgttgtct gtcatttttg gaagtgcgat
      361 ttttgcttta ggagttgcga ttttatataa aaacaactct tctagcggcg gtacgacgat
      421 ccctcccctc atttttaaaa aatatttcca cttgaatact tcgattggct tattttttac
      481 agacgcattc gttgtttctc ttaatctgtt tatttttgga atagaagagt ttctttatgc
      541 tattttatct attgcaatca cttcaattgt catgacttat atcgaaacag gtatcaatcg
      601 aaagaaatct gttatggtca tgagtgaaca ttatttggaa gacatccgcc ttcgcttaag
      661 tgctgagatt ggacgtggcc ttaccctgct agaggcaaaa ggcgggtata atcgaaagtc
      721 aaaagaggtt ttattgatcg ttgtgactga tcatgaattt tcccgaatga aaccattgat
      781 agaagaaatc gatccaagtg cctttgtcat tgtcagcagc gtagcagaag taatgggacg
      841 cggatttact tatcatccaa tcgaatgagt tggtaagtaa atccgctatt tttttgaatg
      901 cgttttcttt agctggtata ataaaagaat ctagtaactg ttcgactgaa aaggagagga
      961 gatctttatg cataaaggaa cggaaatcga ttttgtatta aatgatagtt tgaaagcact
     1021 agaactgtat caaacaatct ttgatgaaat acaactgatt gaggcaacag attaccctag
     1081 aggcaataat gaagtcattt ttaccttgta tggtacgaga ttccatatgc tggatgaaaa
     1141 cacgtctctt ggtttgactg ctcctactgc tgaacatcca aatacgatct ggttcaatat
     1201 catcgtctct gatatcgagg acacacataa taaagcatta cgaaatggct gtgtagagat
     1261 tcaagaaatg acagaaatga tggactttgg tgtgactaac tcaatgttca gagatccttt
     1321 tggttattta tggatgcttc atcaggtcca tcgtgaagta atttttgaag aaagaaaaca
     1381 actttttgag aaatatatgt aatgtttatg atgagattat tttttgtctc tgatattttt
     1441 tgtatatgtt tgctattcga gaaagcgcgt ctgtttcatg tgctttctct tatttatctt
     1501 atgccaatcc atttctttgc tttccacaat tatattgtgt aaaattcaat tgtataaaat
     1561 ataattaaag gtggtaattt tatgaaaaaa atgtattcta cgaaaatgat caacacaggc
     1621 ggacgctctg gagaagtgca taatcccgat cgtacattcg aattgaatat tgcagctcct
     1681 ggtaaacgcg tcgaaaatgc aaccaaccct gaacaattgt ttgcagctgg tttcagtgca
     1741 tgttttaaca gtgcattaga attgatcaaa cagcaaaaaa atatctctgg ggcttctact
     1801 gtcagtgcac aagtttccct ttattcagaa agtgaaacga gctttgtctt aggtgtagag
     1861 attgaaggat ctatcgaagg tctttctctg gaagaaacgc aagaattatt agaggcagcg
     1921 cataaagtgt gtccgtattc aaaagctaca gctggaaata tagaagttac tctgaaagca
     1981 gttagtgaat agactttttt gtgtaaatcc gctatgatac actatagtaa aacagcggag
     2041 gtggaaaaat ggaagatcta tatttagtta atcaagtatg ttttccttta tatgccgcct
     2101 ctaaagaaat cattagacac tacactcccc tcctcaagcc gctgaatctt acctataccc
     2161 agtatcttgt gatgttagtt ttatgggaaa agaaaaagac ttcagtgaaa gagctaggaa
     2221 atgatttgtg tctggattct ggtacgctga ctcccttact aaaaaagtta gcagaaaaaa
     2281 attatattaa tcgtcagcgt gaaacgggtg atgaaagaat cgtaaatgtt acgctgactg
     2341 ctcaaggtgc tgcgttgcaa aaacaagcag aaggtattcc acaaaagatc aagtcttatc
     2401 ttccacttga acaggaagaa ttggatacac tgcaacatct gacccgaaaa atgctagata
     2461 attttcatca agaccagaaa aagggctgaa actgctagat gaacgatttt atgaggaaaa
     2521 ccaactcata agataacgtc atttagcggt ttttcttttt gctctttctt at
//
LOCUS       DAHHZJ010000219         2565 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.223, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000219 DAHHZJ010000000
VERSION     DAHHZJ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.223"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            40..942
                     /locus_tag="NBL02_002728"
     CDS             40..942
                     /locus_tag="NBL02_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCD2573358.1"
                     /translation="MNQSVAKTAHCLNRSRQTIHKVYLFFKQGKSALEYYQQYKKNKS
                     NCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSARTIYRMFKKGLFDSSD
                     LPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHLEGDTIVGLKHKSAVIT
                     LVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFKSITFDCGKEFSNWKQISNV
                     NDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSVDEFFIQSVASKRNNIPRK
                     SLDYRTPLEVFLSYVSIDDLSNLI"
     gene            1075..1281
                     /locus_tag="NBL02_002729"
     CDS             1075..1281
                     /locus_tag="NBL02_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCD2573359.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
     gene            1292..2311
                     /locus_tag="NBL02_002730"
     CDS             1292..2311
                     /locus_tag="NBL02_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HCD2573360.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
BASE COUNT          869 a          443 c          518 g          735 t
ORIGIN      
        1 acaacggatg aacttgtaat aatagagtct tatttcaaaa tgaatcaatc tgttgctaaa
       61 actgcccatt gcttgaatcg ttcaagacaa acgatccata aagtatatct gttcttcaag
      121 caaggaaaat cagccttaga atattatcaa cagtataaga aaaacaaatc aaactgtggt
      181 agacgtccgc ttgttttacc cgaggaacaa tcagaatata ttcaaagaaa ggttgttcaa
      241 ggatggacac ccgatgtgat tgttggtcgt gcagcgtttc ctattagttg ttctgctcgt
      301 accatttatc gtatgttcaa aaaggggcta tttgattctt ctgacttacc gatgaaaggc
      361 aagcgtaaac cgaatggaca tcaagaaaga cgtggaaaac aaactttccg ccgctctatt
      421 catgaacgtg aaaaggatta tagccaattc tcaaatgagt ttggtcacct tgaaggtgac
      481 actatcgtag gtctgaaaca taaaagtgct gtaattacct tagttgaacg attatcaaaa
      541 gttatcatca cattgaaacc gtgtggtaga caagcgattg atattgaaaa aaaattaaat
      601 caatggtttg aatctgtacc gaaaaaccta ttcaaatcca tcacttttga ttgtggaaag
      661 gaattttcaa attggaaaca gatcagtaat gtcaatgata ttgccattta tttcgctgat
      721 ccaggaacgc cgtctcaaag aggcctaaac gagaattcta acggattgtt acgtagagat
      781 ggtttattga aatctatgga tttcaattca gtagatgaat tttttattca atctgtcgca
      841 tcgaaacgaa ataatattcc tagaaaatca ctggattatc gaacaccttt ggaagtattt
      901 ttgagttacg taagtattga tgatctgtct aacttaattt gacaattaag aaagataaaa
      961 ttgaacaact ggacgggcaa attgatgcct tgctttcaga aagagaaata ttgatagaag
     1021 aaaacaagcg attgaaaaag aaagtagccc aaccaatgaa gggaggtgga tagtatgaac
     1081 aatagaacct ttgatatttt gaaatggatc gctatagtag ttatccccgc tttagcgact
     1141 tttgtcggtc ttgtcggaaa aggtgttaac tggcggtaca cagatataac ggtgatgatt
     1201 attacaggtt ttggagcatt tctagggagt attttaggtg tatcaaatcg aacgtataag
     1261 aaaatttcat cagataaata ggaggataga tatgaaaaag aaaattacta ttactgcgat
     1321 gagcctgtta acggctcttt ttttattgcc aattaacgga tttgcctata ctatcaacaa
     1381 tgaatttaat ttgggcgcaa atgaaggtag ctcacaagta gcaaataatc agtatatttt
     1441 actgcatgaa acggctaatg aaacagcaac aggacgcaat gaagcgcagt atatgcaacg
     1501 ttcatggact agcgcttata ctgcttatat tgtgggagac ggcggaattg tttatcaagt
     1561 cggtcaacct ggttatgtac agtacggtgc tggttcgtat gctaatgcca acagtcctgt
     1621 gcagattgag ttacaacaca cacatgataa agcaacgttt gagaaaaact acaaggcata
     1681 cgttgaattg gctagagatt cagcaatgaa atatggtatt ccattaacgt tggacactcc
     1741 ttataaccaa ccgggaatca aatcgcattt atgggtaaca caaaacatct ggggcgatca
     1801 tacagatcct tacggttatc tttctgaaat gggcgtaagt aaagaaaaat tagcatatga
     1861 tttagctcat ggatttaccg atgaaaatcc aacaacttct gaaaacaagc ctgtcattga
     1921 tccaacacga gctggtgcag ctaatcctac actgacagat ggaacgaatt acgcccacat
     1981 tgatcagttt ggagaaatcg aaaatgcaaa tttgcatgta gctggatggc acattgctaa
     2041 ctataaatac gagtatatct tcattatgga ttacaatact gggaaagaat tagctcgagt
     2101 aagagctgat ggaatttata gatcagatgt aaatcaagct tataatactt ctggaaatgt
     2161 tggctatcat gtatctttta acatgcgtaa cttccctaat aagaaagtct atgtcatgat
     2221 gcgggcaacg aatgatccag agggaaacac taaaggcggt gcgcaagatt tccatgacaa
     2281 acgttggtat ttaaatattc ctaaacgata aaaatagctc ctcgttgagg agcattacat
     2341 aactatattg acaactataa aaatcattcg ataaaatagt gatgttatcg catatcttca
     2401 ctatcaccca taatagtcac actccaagct atgcgataac aggtttgttg ccacacattc
     2461 tactggttga ttgtttatgg ctttatgtgg caacaaccag tacccttagc tcagttggtc
     2521 agagcagacg gctcataacc gtccggtcgt aggttcgagt cctac
//
LOCUS       DAHHZJ010000220         2497 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.224, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000220 DAHHZJ010000000
VERSION     DAHHZJ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.224"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(526..1866)
                     /gene="glnA"
                     /locus_tag="NBL02_002731"
     CDS             complement(526..1866)
                     /gene="glnA"
                     /locus_tag="NBL02_002731"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HCD2573361.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(1894..2286)
                     /locus_tag="NBL02_002732"
     CDS             complement(1894..2286)
                     /locus_tag="NBL02_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCD2573362.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          720 a          550 c          418 g          809 t
ORIGIN      
        1 tcgtgcctcc ttatttctaa ataaacggtg ttctcctgcc aactcctcgg tcacaagtca
       61 caatatccat caaacatgag aagctgtttt tgaaattatt tcttgtgatt cggagttaaa
      121 tggtaggctc acaacctctg gataaacggt attcaaaagc cagtttctcg gtattaagcc
      181 aaatttatgc aaaaatgtag aaagccattt tcacaaaatt tttcttaata ctcgaaacta
      241 aacgtctttt tcaaaacctc ttggtaaacg gtgttcaaaa gcttgcatct gcggtaataa
      301 gttcagccaa ttcaaaaaca agaaaagctg tttttgcttg gctattctta ttacttgttg
      361 cagggcagct ttttcacaac ctctccacta aacggtgttc aatcagctga ccctcggtat
      421 tagctcaaaa aacggcaaaa tatgaggaac tatttttccg ttagtttctc taatactcag
      481 gtcaaaacgc tgatttcaca gcctctaatt cacggtattc ctgttttaat aaagttctag
      541 atattgttct ctttcccact cggaaacagt ttgtctgaat gccgcccatt cgagacgttt
      601 tgcttcaaca aaatttgaga agatatgttg tcctaatgcg tcaatcatta cattatcctt
      661 acgcaattct ttgatagcat tatggatagt tgaaggtaag tcctggatat gtgcagcttc
      721 tctttcttcc tcatccatta cataaatgtt acggtcaaca gctgctggcg gtgtgatctg
      781 attacggata ccgtctagtc ctgcttgcaa caatactgcc attgccaagt atgggtttgc
      841 agatggatca actgaccgca attccaaacg tgtggataaa ccgcgagatt ctggtacacg
      901 tactaatggc gaacgattac ggccactcca tgcgacataa acaggcgctt cataacctgg
      961 aacgagacgt ttataagaat tgactgttgg attgcaaacg gctgtgtagg cacgagcatg
     1021 tttcagcaaa ccacctaaga aatgataagc tgtttcactt agttccaatt caccgtcttt
     1081 atcatagaaa acatttcctt catcattgaa caaagacata ttgcaatgca taccagaacc
     1141 attgatccca aataacggtt tcggcatgaa agtcgcgtga agtccatgct tgcgtgcaat
     1201 cgttttgaca acgagcttga aggtttggat attatcacaa gcatctacca catctgcata
     1261 tttaaagtca atttcatgct gtcctggcgc tacttcatga tgtgaagctt ccacttcaaa
     1321 gcctagactt tctaattcca acacgatgtc acggcggcaa ttttcaccta aatctgttgg
     1381 tgcaaaatca aaataaccac ctttatcatt caaggtcgtt gtgatcccac cgtcttcgtc
     1441 taatttaaat aagaagaatt ctggttcagg tcctaaattg aaagaggtaa aaccaaggtc
     1501 gcgcatatct ttcaatgctc gtttcaaatt cccgcgagga tcgccggcaa atggtgtccc
     1561 atccggatta tagatatcac aaattaaacg agcaactttt ccatgagcac tttcccacgg
     1621 gaagatcatc caagtggaga gatctggata caaatacata tcgctttctt caattcgaac
     1681 aaatccttca atcgaagaac cgtcaaacat cattttattt tctaatactt tgtcgatctg
     1741 gctcactgga acttctacat ttttgatcgt acctaagatg tctgtgaaca tcaatcgtaa
     1801 aaatcgtacg ttttcttcct caatgattcg tttgatttct tcacctgtta tctgtttctt
     1861 taccatggta tccctcttcc ttttttaatt tattcagtca aagaattgtc tttgcaatcc
     1921 ttgcgactga taatgacctg ttttgcttaa cccaccttgc gccaaaatct catcacgaaa
     1981 aatttgtcga acgtcatgat ctgtcaacgg cttcttgttt tttgcttctt gttttttcgc
     2041 ctgtgccatt tcgtatacac gtttgatccc agccatattc aatccctctg aaagatagtc
     2101 tttaatctca agcaacatat caatatcatt cagcgaatac atacgtcggt ttccttcgct
     2161 tcgttccgga tgaactaaat cttgttcttc ataatagcga atttgccgag cagacaaatc
     2221 agtcagcttc ataactgtac caattggaaa tacggacata gatcggcgca attccttttc
     2281 tcccatcaga cgtccccttt cgttttataa gttggctaaa gtgtaccaat taaaaaaaat
     2341 attgtcaact gcatatgtta ggttttctaa catgagatct ctatttttta taaaataaca
     2401 aaaaaataag aacacaaaaa aggtatctag ttgattttcg taccagatac cttttatttt
     2461 ttattatact gtactatcct ttattagtgt ttttact
//
LOCUS       DAHHZJ010000221         2452 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.225, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000221 DAHHZJ010000000
VERSION     DAHHZJ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.225"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(893..1411)
                     /locus_tag="NBL02_002733"
     CDS             complement(893..1411)
                     /locus_tag="NBL02_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HCD2573363.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT          796 a          449 c          355 g          852 t
ORIGIN      
        1 atactaaagt tatttgttct tttattaaat acacctacaa cttataaatt gatggtattt
       61 agataacacg aaagaataaa aataacagct gatagtaaag tttgttgacg attgctactc
      121 aatcgaaagt gagttagtgc gtatgaactt gcttctgaaa acttatgaaa tgaaagacct
      181 tttacatctt gcagaaatcg gtactgggat taaactaagc aataaaaact aatgttcccc
      241 tcattctttg aacttctact atttgattta tttattctga taaatgacga tttcaccttc
      301 aggatcttca aatcccgtat agaatttatt taagatctct tcagtatcag taataggata
      361 ggcgataggc cacataccct ttgatggtta gaataaactg ctcaaaatct gtatcgtatg
      421 ttggctttag catattaaca aaggctatct ccaaattata aaccataagc gttgaatcgt
      481 tcttaacaat ttcaattctt cctaacccaa agtcagaaga tataattacc ggacttgcct
      541 tacccttcat gtgggttgtt gagattccat atttaaaggg aattgtcggt actatcggcg
      601 gtctgaaaaa catcccaaaa ataaaaaaat ctttcccctg ctagtctcct aagtcccttt
      661 gtagcggata tggcaccata atctcatttt atcgaagtga gtcatggttt atctgttggc
      721 caaccaaaat tgatacaatc taacgatgca ccctctacgg cattctaggg gagtgcactt
      781 gcattcaccc aataaaaacc ggcagttagc aaagaactta tgtagtcgta tgttcttact
      841 gttaacatac aagaggtctc ctttccacaa ttgcttagca gtaaacggga atttacaaag
      901 ctaatctctt ccattttctt atctttgttc taaaagtccc aaatggagca actgtattta
      961 catgaataaa tttatatact tcccatacag ccgttttggt tgcctcgtct gcccattttc
     1021 ttttgtgtgc agtgaataga tcttcatcac tcattgaatc aatcatcatg ttaatatcat
     1081 caatattacg attgagaatt tcctctaatt ctttcaatgg tagatgtgca taggtatttg
     1141 taaaccactt atataactct cctaactgat tccatttaaa ttgatcagaa ggcgttttaa
     1201 cagataatcc atttctttca tcattttccc attttagaat taaattcgtc caaccaactt
     1261 gatatgctaa attttcagca ggtgttctat ccacttcctc aactctttta tccttcaatt
     1321 cttcaggaat agtttcaaat tctaaaatat atttagtata agtcctattt atttcttttt
     1381 ttaattcttc tttattttta tatgttctca ttatattacc tccataaatt cctaattgtc
     1441 tcctttaatt catctataat ttcatccacg atattttaat ccgttctaca atgcatatct
     1501 gatatgcatt gactgtctaa ctcatatgtg aatgatttat aaaatatccc aacgtctcat
     1561 ctaaagaaat gttgatcgtc agcaaggagc taaaaaatac gaatatattt cttttgtagt
     1621 aaaatcggac tggtttgcat atcattttct tcgtaaatat caaaatcatg ttaactacta
     1681 tcaattacta ataaaaacta ccactttaag cgttaagaac tgttaagttt agttaagtga
     1741 ggatagttca caaaatccat tgttaaactt cacgaaagtg gtaaaactca aaatgcttta
     1801 tataggatta cagcatcgct ctttcttcga ttgcgcgttg ggtaaaaaat attatgaagt
     1861 aaaaattgac gatgacacgt tccttacggc tcatcagatg aaacagctac aaaaacgaaa
     1921 tgatcaactc gaagaaaatt taattctaaa aaagcgattg ccatattcac gcctcgctcg
     1981 acgaactatt aaaggtcaag atttacctga agatcatcct ttttaggaaa ttgaagcttt
     2041 ttatgttgca caatgcattt ataatactac aatgatgttt tcaccagata ttcttctagg
     2101 gtgcggtgta atgaaacaga tacacttgca aaagaaaatt caccgcgaat ttactaaaat
     2161 tatgaataat tatgttaatc ttccgaaatt tttttccaaa attaagggat caagctggtg
     2221 ttatagattg tttaaggttg gcaaaaaaat aacttctttt taaacaatta tagaaaaccg
     2281 atactctgtt aagttggttg acacggtgtc gatttttatg actttcttat tctgaaactt
     2341 cactaacgga cagtacctaa aaagtctata gtatggacgt ttacgagtac tattttatca
     2401 aaaatgctgc tcatttcaac ttatatgttt tagtgaacta cccccactta gc
//
LOCUS       DAHHZJ010000222         2444 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.226, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000222 DAHHZJ010000000
VERSION     DAHHZJ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.226"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(<1..132)
                     /locus_tag="NBL02_002734"
     CDS             complement(<1..132)
                     /locus_tag="NBL02_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCD2573364.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
     gene            542..1686
                     /locus_tag="NBL02_002735"
                     /pseudo
     CDS             542..1686
                     /locus_tag="NBL02_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
     gene            1857..2315
                     /locus_tag="NBL02_002736"
     CDS             1857..2315
                     /locus_tag="NBL02_002736"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCD2573365.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
BASE COUNT          816 a          456 c          477 g          695 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaaatgata ttttacgagg aggaaagaaa
      541 catgcacatt caaacaaaat tgatccacgg aggcatcagt gaagatccca caacaggagc
      601 tgtcagtgta cctatttatc aaacatccac ttatcggcaa gatggcgtcg gccagcctaa
      661 acaatatgaa tattcaagat ctggtaatcc tactcgtttt gcattggaag aactgattgc
      721 tgatttggaa ggcggtgttc gaggattcgc tttttcttct ggtctttctg ggattcatgc
      781 cgttttttct ctttttcaag ctggcgatca tatcttatta ggagatgatg tctatggagg
      841 aacattccgt ctatttgaca aagttcttac taaaaatggt ctcgagtaca cgatcataga
      901 tacgagtaat cttgataaaa tagaacaatc catcaaaccc aatacaaaag cactttatct
      961 tgaaacacct agcaatccgc ttctaaagat tactgattta gaaaaaagtg ccactctggc
     1021 tcatcaacat ggattgatag ttattgctga caatacattt gctactcctt attttcaaag
     1081 acctttagat ctcggttctg atatcgttgt ccatagcggt accaaatatc taggcggaca
     1141 cagcgatgta gtagctggtt tagtgacttc taaccaaaag atctagccga tcagatcgga
     1201 ttttaccaaa atgctatcgg agccgtactt ggtccccaag atagctggtt acttcagcgc
     1261 ggaatcaaga cactaagtgt tcgtatggag gaacatcaaa aaaatgcttt tgtggtagct
     1321 gattttctat tttctcatcc agcagtagaa aaggtctact atccaggatt acccgatcat
     1381 gaactccatg gagttgctaa gcagcagatg agcggcttta gcggaatgat ttcttttact
     1441 ttaaaaaatg aagaatctgc tattccattt gttgaatcac tccaactatt tactttagca
     1501 gaaagtttag gtggcgtaga aagtttagtg gaaattcctt ctgtcatgac tcatgcgtct
     1561 attccaaaag aaaaacgaga agaggctggc ataaaggacg gcttgatccg tctttctgta
     1621 ggaattgagt atggtcagga tctcataaac gatttagctc aggcatttga cagaatcaag
     1681 aactagaagt cccaataaac gacttagcag aaaaatctaa aaaatatttc agattgctac
     1741 accttggaaa cgataagtgt tagaatcaag tagaaagacg tttttgtaaa tttcactaaa
     1801 taattcttta gtgaaaagca tcaaaacgaa tgaacaaaca gataggagtc ttacaaatga
     1861 gaatgaatgt cgaaagtttt aatcttgacc atacaaaagt caaagcacct tatgtacgtg
     1921 tagctgatcg aaaaacgggt gcgcatggcg atgtcatcat taaatacgac gtccgcttta
     1981 aacaaccaaa taaagaacac atggacatgc caagtctaca ttcacttgag cacttgactg
     2041 ccgaactaat ccgcaatcac gcagattaca tcgtagactg gtctccaatg ggatgccaaa
     2101 caggctttta cttgactgta ttgaatcatg aaagctacga agacatttta gatgttcttg
     2161 aagcaacaat gaaagatgtt cttgaagcta ctgaagtacc tgcaagcaat gaaacacaat
     2221 gtggctgggc tgccagccat acactagaag gtgcacaagc tttggctcgt gaatttttag
     2281 caaaacgcga tgaatggtct gaagtagaag catagtttct aaaagaacgt aaagatgaga
     2341 agcaatatag ctatcttatc ttcgtatttt attaaatatt tattgtctga aaagagttct
     2401 aaacgggaaa acaaatagct agttttcagt ttagcgctct tttt
//
LOCUS       DAHHZJ010000223         2441 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.227, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000223 DAHHZJ010000000
VERSION     DAHHZJ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.227"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            673..867
                     /locus_tag="NBL02_002737"
     CDS             673..867
                     /locus_tag="NBL02_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573366.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            989..1307
                     /locus_tag="NBL02_002738"
                     /pseudo
     CDS             989..1307
                     /locus_tag="NBL02_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028215.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1551..2381)
                     /locus_tag="NBL02_002739"
     CDS             complement(1551..2381)
                     /locus_tag="NBL02_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HCD2573367.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
BASE COUNT          722 a          434 c          484 g          801 t
ORIGIN      
        1 ggattcctaa ccatcttatt agcttttata gggtatatgt tggttgggct gctaaaatca
       61 tacagtaatg agttgctgaa tttttcaaca tttattaatg atacattagt tccaagtttg
      121 tttttcattg tattttttgc tgttggttat tttataataa agatatgatg actaatttag
      181 ctcaactatt ccagcgtcag ctgaagatat tgcaattccc gattccttaa tcatttatct
      241 ggatgagaca gaaagtgaag ttgcaacaga tgtgcatgta gttgaggata aaaatgatag
      301 tttaaaaatt aatagttcag atactttact aagagtaagt cctatctttt cagctgttat
      361 ttagatgcaa gtagcaacta agttaggtag tactcctgca atggataggt atttgtgtgt
      421 tcgatatata agtggtcgaa gtatcaaggt tatgtatact ggatgggaga tagaagagtt
      481 aaaaattgga ggtcaccgcc acaaatggaa tttagattta gaggtacctt agatttggta
      541 tagttattaa acacaagatg gcctggtttg tagggaaagt cgaagagtat ttttccactt
      601 ctgcgcccct ttaatttttc tagtaatgcc gtgaatttgt gattaattgg aaatatgaag
      661 aaaggagagt ctatgaaaac aacactagtt ctattctata aaaagcatcc ttattttaca
      721 cttctgataa atatattgtt ggctagtgtt atcgggattt ctgttgaata tctaataaat
      781 aaagatttca taggttcttg tttttatacg gctttatttc ttggacttct tgaagcattt
      841 tccatttata aaaaatcaaa aaaataaaat agattgccgt aagagatctt tttcatttca
      901 tttttttctg aaaacccgtt gtttctgtca gtgaagcagc gggtttttag atttattgtc
      961 tttagaccta attgagcgcg ccgagcgatt gaaatacaaa accacttgct atgcgggtgg
     1021 agcaataaag ggaaatagtt tagcccgtac aaagtagatg tgtaaatacc acattgtatt
     1081 cacaccaaag tatagaagaa aaataattta taatcaatat cgagccaata ttcaggagat
     1141 catcaagaga ttgtgcaaat ctaaaatata aatttgggta tcgacacttt tgggtggaag
     1201 gatactatgt gacaaaagta ggacaaaatg aagcgaccat caaaaagtac attcaagaac
     1261 aagaaaaaca tgatagtgcg ttagataagc tgagtgtacg agagtaagac gatcctttta
     1321 agggctaagg agtagtacat aaacctttta agaggtgggc aaaactggca aaggcaatat
     1381 ggcttgaaca taaaatatga aagccagcgt ctttaggcgc tggccggtga tttgagctta
     1441 tagctctaga gaaaaccacc cttttgaagg gtggttatga ttaatgactt tagtcagttt
     1501 tgctcacaaa gcgtgtggaa caaaaaacgg gtgtttctac gggtagatat ttatttcttg
     1561 aatggaggtg tcccatacat ttttggtgtt actggttcta attttggtgc agcgccttcg
     1621 atcttaggtt ctttcaacca ttgatattcg ccttcgccat ccagtgcttt gccactagcc
     1681 caagaacctt cactactttg gtctccctct gagaagttat agaagtcata agcaatgtct
     1741 tttcgttctt tgtcacgcgg gaaagtactt gggactaaga ttgaaccttc tttttcttct
     1801 aattctttca ctgcacattc ccatagattt tgatgatagc tatctcttgc aaggagaaca
     1861 gacagcatat cttttacgcc acgatcatcg atcatttcgt aaatacgtgc tacttgaagt
     1921 cttccttgtg attcggcatt taaattagct ctaaagtctg ctaacaagtt gccactcgca
     1981 atgatatagg caccgctcca tggattgccc acgctgtcag caggacgagg tcctaagcca
     2041 gctacgatcg cgtgctgtgg attcattcca gagataatgg caccgattgt tggatctttt
     2101 tgcatcgctg cttcttgatc ttcaacaggc gcaccctcta ataaacgagc aaccattgtc
     2161 gccaacattt ccacgtggcc gatttcttct gttgcaatgt ccatcagcat atctttatat
     2221 ttttcttctc ctcggcagct ccagccttgg aacaggtact gcatagctac actcatctcg
     2281 ccatattggc cgccgatcag ctcctgcaga tatttcgcga ttaatggatt cggacgatct
     2341 ggttttgctt caaactgcaa ttctttctgg tgtctaaaca tcaaaattcc tcctaatcta
     2401 tttttaatac attctcattc tgctacgaaa gattaaaaaa t
//
LOCUS       DAHHZJ010000224         2435 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.228, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000224 DAHHZJ010000000
VERSION     DAHHZJ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.228"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..1161
                     /locus_tag="NBL02_002740"
     CDS             <1..1161
                     /locus_tag="NBL02_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCD2573368.1"
                     /translation="KLDDSSSYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRT
                     RFLCHDCGRTFSAKTDLVDEHHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTR
                     ILREATKHYQPRMNFLPTVLCMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYK
                     LTQYFLRFPRKARLKVKYLVMDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQL
                     RVQIMNRFRTSHSENQKKYRRLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVD
                     ELLSYNEELRTAYHFCQLLRYYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQS
                     GISNAFKVSHSNGVTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            complement(1448..2338)
                     /locus_tag="NBL02_002741"
     CDS             complement(1448..2338)
                     /locus_tag="NBL02_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HCD2573369.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT          675 a          526 c          458 g          776 t
ORIGIN      
        1 aaattggatg actcttcttc ctattgtgaa cactgtcgat cggttcgtat tatccgcaac
       61 ggttcctata cgacacgcac tcagatgttg aaagtcaaag aaaagttaac tattttagaa
      121 ttaaaacgta cccgttttct ctgccatgat tgtggaagga ccttttctgc gaaaacggac
      181 ttagttgatg aacatcatca actaacaaag gaactgaagc aggcgattct catggaactg
      241 tacgaaaatc aatcacgtaa actaatcgct aagaagtact tcgtctcgga tgggacggtg
      301 acacggatct tgcgtgaagc aacgaagcat taccagccac gtatgaactt ccttccgacg
      361 gtgttgtgta tggatgagtt caaatcaatg aattctgttt ctggttccat gagttttatt
      421 tgtgttgatg ggacaaccaa tcagctattt acgatccttg aagatcgcag actttacaaa
      481 ctaactcaat acttcctgcg ctttcctaga aaggcccgtt taaaggtcaa gtacctagtg
      541 atggacatga atgccagcta ttgtcagttg cttaaaacag tctttccaaa tgcggagatc
      601 gtaactgacc gctttcatat tgtccaacac atcaatcgca gcttcaatca attacgtgtc
      661 cagatcatga atcgttttcg aacctcacac tctgaaaatc agaagaagta ccgtcggctc
      721 aagcgatatt ggaaactctt actcaaagat tccacgacat gggagccact caaacggcat
      781 tatcatcgac tgtttaaacg accaattagt caaacagaaa tcgttgatga gctgttaagc
      841 tacaacgaag aattgcgaac tgcgtatcat ttttgtcaat tattacgcta ttatttcgtt
      901 aaacgaggta cggaaggctt tcaagaaacc cttaaaacaa tttcatctac gttacctcag
      961 tcgtttaaaa agaaattcac gatcttccat tgctaccaat cgggtatttc aaatgccttt
     1021 aaggtgtccc actcaaacgg cgtcactgag gggctgaata acaagatcaa gctgattaaa
     1081 cgaattgcgt ttggttatcg caacttttat aactttcggg cacggatcta tttacaacaa
     1141 ggtctgatct ttgaaaacta aaaaaatatg cggcgcacag aacgcgccgc atatcggact
     1201 aagttttatt caaatttgtc ttcaccaaca cgatttgacg aagagccaaa aaaagttacg
     1261 acagttctat gtcgtagctc tttttttcga ataaacggtg ttcaatcagc tgaccctcgg
     1321 tattagctca aaaaacggca aaatatgagg aactattttt cacaattaaa ggaaaaatgt
     1381 atatgtgggg aaacatagtt gatttttcct taatagtaac gactaccgtg gcatgtttat
     1441 ctgctagtta tttgatattt ccttcgtgat cacgttctac ttccatcatt gtgattggta
     1501 aatagcccaa ttctttgaca ggtccttctt ggatttcttc tgtcatcatg tagtctaaga
     1561 atttttggac ttcaggggaa ggttttccgt tcgtgtacat atgttcatat gaccagattc
     1621 cccagctgtt atctgctaca ttctcttctt ttggttctac accgtcgata cttagtgctt
     1681 gtgtggaatc atcgagatat gagaaagcta aatagctgat tgctcctggt gtttgactta
     1741 ccagctgccg aactgttcct gatgaatctt gttcttggga ctgcacaggg gtagcaccat
     1801 ccagtcccca tttttcgaat gtcgcgcggg taccgctgcc gtttgcacgg ttcacgacgt
     1861 tgattttttg atctttcccg ccaacttcct tccagttagt gatttttcct gtaaagatat
     1921 caatcaattc ctgtttggtg atatctttga catctgtatc tttattgacg attggtgcca
     1981 tcccgaccac tgctacttta tgatccacta attttgaagc atcaactccg tctctttctt
     2041 ctgcaaatac atcagagttt ccgatagtaa ctgctccagc ttcgacttgg ctcaaaccag
     2101 ttccgcttcc tcctccttgt acagagattt gataatttgg attttcttgt acaaagcttt
     2161 cttgtgctgc atcgactaac ggctgtaatg ctgtcgaacc taccgcaacg atttttactt
     2221 gttcatttga gtcacttgaa ttattttgtg tggcagagcc atctgtattt cctgatcctg
     2281 agccgcatcc ggctaaaaga attcccatca tacctagagt aaacaatact tttttcattt
     2341 tattgctcct ttcttattta caactaaagt ttatcagtcg actgtaaatc cctcgtatgg
     2401 ttgctgtaaa aatatagtta agttatgtaa aaatc
//
LOCUS       DAHHZJ010000225         2420 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.229, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000225 DAHHZJ010000000
VERSION     DAHHZJ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.229"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(<1..741)
                     /locus_tag="NBL02_002742"
     CDS             complement(<1..741)
                     /locus_tag="NBL02_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCD2573370.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWE"
     gene            complement(764..>2420)
                     /locus_tag="NBL02_002743"
     CDS             complement(764..>2420)
                     /locus_tag="NBL02_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HCD2573371.1"
                     /translation="LFVNMGSSRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQ
                     TESKIPLLIAANTEAGGNGACTDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGC
                     NWSFAPIVDINRNWRNPIISSRSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDG
                     IDERDQHLSFSINSLGTEEWDNSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLK
                     DEDLLPATLSKELITDLLRGKLGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDL
                     FLFFNDPDEDFNYMMEGYRKGIITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQE
                     ALEKIGLIENKSIFKEVADQAITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVV
                     AGNRPQAIDLLKQILSAKGFEVEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLT
                     KKYDLIINIANVNPGTVQRIVWPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVK
                     TYINTYDGKQETLEILVDKLMGLSEFKGVSPIDAYCGLKDTFI"
BASE COUNT          752 a          491 c          308 g          869 t
ORIGIN      
        1 ttcccaaaaa attggattct taagctgaat cattcctttc tttccttcag cttcagctac
       61 aagttgagcc tcttcgtcat atatcgctat taagattttt ccggtccatt ctgtatcgat
      121 attgaattct acacttgcat ccaaatcagt tattcttgta ttaatagtaa tatcacttat
      181 atagtctttc ggagttgtat ataacttaac aggtctatga atacctgcat aattgaagaa
      241 atcaaaatta actaagtttt ccttttttat atccccattt tcctgtactg tttccttcat
      301 aatccccaca ggtaaagtac tatcgtcaat aatattgttt acagcaactg taattcgatt
      361 attcccgtct tttacaaacc gattaatttc agactcaaat ggtgtaaacc cacctttgtg
      421 ttgagtaata aactttccat tcaaaaaaac ttttgcttca tgtgttgcag atccaaaacg
      481 aagaactaat ctttcttcta ataatctttt aggaagagtc acttcttttt cataccacac
      541 ccatccaaca tgatttctaa tgttctctgt ttcaaataaa tcattataag aagagggaac
      601 tgaaatcgga taactctctt tttcatttat tggttctaaa gcaagttctt cctgtaagtc
      661 atcatttgta tctaatttaa atctccagag accgcttaag tcaattactt gcctcgtctc
      721 tgtcataatg ggatacaaca tataaaccac tcctatctaa atattaaata aatgtatcct
      781 ttaaaccaca ataagcatct attggactta ctcccttaaa ttcagaaaga cccattagtt
      841 tgtccaccaa tatttctaaa gtttcttgtt ttccatcata agtattaata taagtcttta
      901 cttgaggtac atcagccaaa tgaaacggac actgtacaga tacaaaaatt gttggaattt
      961 catgaatgta aaacggtata tccggagttc ctttacttgc tggccagaca attcgttgaa
     1021 ctgtgcctgg attaacatta gctatattaa tgatcaagtc atatttttta gttaaatttt
     1081 taattggtct cttagcagca tatattccag ctatattttt aattttttct tcttgcgata
     1141 atcctttcaa ttcttcttca ggtgaaatgt atttttcaac ttcaaatcct tttgctgaca
     1201 aaatctgttt caataaatca atggcttgag gtctatttcc agcaactact tcaccaaacc
     1261 caccttttac cccatgtaca tctactagta aaatcctttt atatttttct ggtacaatag
     1321 gccaaatatc ttgtttatct ttaactaatg taattgcttg gtcagccacc tctttaaaaa
     1381 ttgatttatt ttcaattaaa ccaatttttt ccagagcttc ttgcttaggt aataaaattt
     1441 gttcttttgc ttttttgtgt aatcccaatt tagcttttaa ccctaaaatt ctttttaagg
     1501 catctaataa ccgatcatca gtgataattc ctttacgata gccttccatc atataattaa
     1561 agtcttcatc tggatcatta aaaaatagaa atagatcaca tccagctgca atagaagtgg
     1621 gcaataattc tgatcgtttc attgccccag tcattcctaa catatgacta gcatcagtaa
     1681 ctactaatcc attaaatccg agttttcctc taagtaagtc agtgattaat tcttttgata
     1741 aagttgcagg aagtaaatct tcatctttca gatcaggatt aaaatgtttt gaataagctg
     1801 gcatatggat atgacctgcc ataattgaag gtaatccagc ttctattaag cctgagtata
     1861 cttttccaaa agaattatcc cattcttccg tacctaaact attaatacta aaagatagat
     1921 gctgatctcg ttcgtcaata ccgtcaccag ggaaatgttt agctgcaggc aaaattccac
     1981 tctcctgaat acctttcata taagctaaac taagttctaa aactttatca gggttttgtc
     2041 caaaagatcg actagaaatg ataggatttc tccaatttct attaatatct acaataggag
     2101 caaaactcca gttacatcca atagccgatg cttcaatccc agaaactctc cctaattcat
     2161 aagcatattt cgcatcattt gttgcaccaa tttttacttc tacaccaact tcagtaccat
     2221 ctgtacaagc tccgttacca cctgcttccg tattagcagc aattaataga ggaattttac
     2281 tttctgtttg taagatatag ttttgatcat atacctcatc agcagttcct ggattatatc
     2341 gtacagctcc aatatggtac tgattgacca tattactcaa gtaattttta tcacgactag
     2401 atcccatatt aacaaacaat
//
LOCUS       DAHHZJ010000226         2415 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.230, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000226 DAHHZJ010000000
VERSION     DAHHZJ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.230"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            115..2268
                     /locus_tag="NBL02_002744"
     CDS             115..2268
                     /locus_tag="NBL02_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HCD2573372.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          823 a          425 c          535 g          632 t
ORIGIN      
        1 tgattttatc ttgaaattag gtaaagaatt tcttgctccg cccatttttt cttttttatt
       61 aagaagtttt tgggtacaat gaagcatgaa tgtaaatagg cggaaggata ggaaatgatg
      121 aaagatttta tgaaaaaaat attaaatact tcttggttag aaaaaataag aaaaggtgca
      181 caagaaccta agaacaaaac gtttcgccgg tcccatgttc cttttcgctt aaattttctt
      241 tttttcatta tatttacgtt atttgttgcg ttgattgcta gattgggtta tttgcagatc
      301 gtgaacggcg aagagatgga ggcgcgagtg aagtctactt ctacgattac ggtccaagaa
      361 agtactcctc gcggaatgat ctacgactcc acaggaaaag ctttagttac gaataaagcg
      421 aatcaggcca tcacatatac gagaggaaca caagttacgg ctaaagattt gcttgaaatc
      481 gcaacgaagc tgaactcgct gatcgatgtt ccggtagatg aaaatctgac agatagagat
      541 aaaaaagatt tttggctggc tgatcccaaa catttgaaag aagccacaaa aagactttcg
      601 gcaaaagaaa agcaattagg gaacagcgaa caatatagtg cgatggtcaa taaagtcaca
      661 gctgatgaga ttaatttaaa cgaggagcaa ttaaaaattg ctaccatctt taaacggatg
      721 aatagtgcat atgaattaaa cactgtctat atcaaaaact ctggtgttac tgatcaagag
      781 ttagctgttg tggcagaacg cgcttctgaa cttccaggag tttcgactgg cacagactgg
      841 acacgagaat ataatgcagc aagttcactg aaaagtattc ttggcagtgt gacgactgaa
      901 aagcaaggac ttccagcaga tgaagcagag aaatacctag caaaaggtta ttctcggaat
      961 gaccgtgtag gacaaagtta tttggaaaaa caatatgaag atgtcctgca aggaaccaaa
     1021 acccaatatg aagtttccct tgataatgaa ggaaatgttt cgaatcaaaa agaaatcttc
     1081 tctggggaaa aaggttctaa tttaatgtta tctatgaatg cagaattcca atcaaaagta
     1141 gaagaaattt tgaaaaggaa ttatcaaaca ttgatcaaca acggcaaagc acagtattcg
     1201 cctggtgctt atgcagtggc tatgaatcct cagacaggag aagtgctagc aatgactggt
     1261 ttcagtcatg aacaaggttc caaagaaatc accgagaatg ctttaggcac gattactagc
     1321 gcctttgatc cagggtctgt agtcaaggct ggtactttga cagcaggctg ggcaagtaac
     1381 gcgatcagcg gaaatcaagt tttgattgat gagccgattc gtctgcaagg tgcttcagaa
     1441 aaaagctctg tctttaatcg aagcggacaa gttgctcttg atgcagtcaa agcatcggaa
     1501 ttgtcttcta atacgtatat gatcaaagtg gcgttaaaga tgttaggact agactataca
     1561 cctggaatgg gactacctag tttagatgaa gaagctaaag cttatcagca attgagagac
     1621 tctttcaaag agtttggtct tggtacaacg actgggatcg atttgccgaa tgaatctcct
     1681 gggatctcac gttctgttga ttatatgaag aagttcaatg cggataatgg caaagaatgg
     1741 tatgctccag gtaactttac cgatttggca tttggacagt ttgacacata tacaccaatc
     1801 caattagctc aatatgcttc aacagtcgct aatggcggga aacgagtaca gccgcaccta
     1861 gtcaaagcga tctatggcaa tgacgaaaat ggaaacttgg gtgaagtgaa gaaagaaatt
     1921 ggcacaacag tagaaaatac ggtcaatatt tctgcagaaa atatggcgat cctccgtgaa
     1981 ggattccacc aagttgtcca tggtaccgat ccttacacaa cagccaaacc attagccagt
     2041 gcgaaaatgg acctttccgc aaaaacaggg actgcagaaa cggttgcgga aggtcatcca
     2101 gatattgcga cagtcaatag taatatcgtg gcatacggac caacagacaa tccggaaatt
     2161 gccatcagtg ttgtcttgcc gaacttatta gacgaacaag accacataaa cttgacgatt
     2221 gctaaagaaa taatggacgc gtattatgat atgttcatgg caaaataaaa aagaggtcgt
     2281 gaaaaagccg cttagtttcg agtatgaaga aaaattttgt gaaaatagct tctcacattt
     2341 ttgcttaaat ttgacttcat accgagaaac tggcttttga acaccgttta ctcggcaaaa
     2401 aaggttgtga caaaa
//
LOCUS       DAHHZJ010000227         2400 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.231, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000227 DAHHZJ010000000
VERSION     DAHHZJ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.231"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            45..281
                     /locus_tag="NBL02_002745"
     CDS             45..281
                     /locus_tag="NBL02_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HCD2573373.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            300..515
                     /locus_tag="NBL02_002746"
     CDS             300..515
                     /locus_tag="NBL02_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HCD2573374.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(1218..1970)
                     /locus_tag="NBL02_002747"
     CDS             complement(1218..1970)
                     /locus_tag="NBL02_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD2573375.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(1985..>2400)
                     /locus_tag="NBL02_002748"
     CDS             complement(1985..>2400)
                     /locus_tag="NBL02_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCD2573376.1"
                     /translation="LKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGII
                     LDNAIEASIESQDRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQG
                     LGLSSIEEINKKNPNMFVNYQNDPYQFTTQVILLF"
BASE COUNT          776 a          386 c          403 g          835 t
ORIGIN      
        1 acgtatgtta cagtagcacc gtaaaaaata ttatttaaag aagcgtgttt aaacatgcaa
       61 aatgtaaagg aactaagtat tgtggaaatg cacaaactgt tggtggaggc aataatgctt
      121 ggggaaaatt ggggcaagtt gtaggtggac ttaccacagg ggcagtaggt ggtgcaggtt
      181 taggaacagc aatttgcgga ccagcttgcg gtgttgttgg agggttatat ggagctgtcg
      241 ctggaggtgc tgcagctggc tgggatgctc gaaaaaaata aaaaaggaga ataagtagta
      301 tgcatattaa aaacacaaaa acaacattta tattatcttc agaagagttg aaaaatattc
      361 aaggtgggag tgccgtggga gttttaggca ctactttttc tggggcaaca gcaggagtaa
      421 aactttgttc agccggagga ccttatgcaa tagctgcttg tggtgtaggt ggagctttat
      481 taggtgctgg attcagcatg tggaccggtg cgtaaacaat taaaaaatat caaacctcaa
      541 gattggtttg tgtcgattac gaacataggt atgttcttta tcttttcaaa agtttggaat
      601 ggacagaact ttttactaaa gcttcttgtg ctagttttta taattatatt aaatttttat
      661 cttttattaa agtcagataa aaattctttg gaaatgtaat ggtagagata tttttacaaa
      721 aggacttgca gcgctgtagt tggtgctgca ggaggaccta tttcggccgt tacaggaggt
      781 attggtaatg tacttgtcgg atgtgcatta aagcctgttc ctgcaagtta aatttataaa
      841 aacaggaaag taattatctc aaatcctatc aagtacaatc ataggattgc acttgacagg
      901 acaaattttt taactaaaaa atgaaaaaca ctttctaaaa attagtagaa tttgccaaat
      961 ctttcgcatc ttccaccata catttaattt taattcccct ttatggtact taataaaaac
     1021 aagaaaagaa ctattcattt ggcccttttt tggttccaga ccatagaaat attcctccag
     1081 taactaatat tgctgttatg cacgtaacta ataacagcta taaaaattgg tacaacttta
     1141 ataggattca gaaaaatttg cactagtgaa ggaatctatt ttattagaaa aatgcaatct
     1201 ccctaaagaa aaaaccacta aacagtgctt tctttcaact tctccttaat tttttgtgct
     1261 ttccctaaag taaagtttct ggccaactct gaatcaaaat atagtgaacg ttttttaaaa
     1321 tctatttctt taacgttaaa gatatttact aaacacgctc gactaattct tgttaacata
     1381 ggatatttct tttcaagctc actaattctt ccataaaatt cgtactgccc atctttagta
     1441 cacaatgata atctatgtgg taattttgaa gcttctacaa aataaatgtc atttatatca
     1501 aaagtaaatg tttgagaacc tatggaaaaa acaaatgctt gattcgtaac ctgttttgtt
     1561 gcatctatcc tttcttgcgc taataacaat aactcaacaa tctcgtttcg atactcatct
     1621 agtgtttgat cttttgttac aaatcctaac gtttcaactc ttctttttat tgttacgggt
     1681 aacatctcat catgagtagt tgtaaaaatg atttttgctt gaacatcata ttttctgatt
     1741 acttctgcta attctatacc gttaacttca tgatttaaat caatatctaa gaaatatatt
     1801 ccttgctttg gtcggaattg tttgaggtat tttttaactt ctaacggact ttgtgttttc
     1861 aatactatct taaatacttt atcgtgaaaa agaatgaaat tatcaataat tcgttctaac
     1921 tgatttaatt gtataatttg atcttcacat aatattattg gataactcat aaatcctcct
     1981 tccctcaaaa caacaaaata acttgagtag taaattgata cggatcgttt tgatagttca
     2041 caaacatatt gggattcttt ttattgattt cctctatact agataaacct aacccttgat
     2101 gtcccttttt tgttgttatt ccttttttca ttagagtatc tatagagagt agtatctctc
     2161 tacaactatt cgatatcgaa atttctatct gtttatcatc tcggtaaata gctagagaca
     2221 gttctctatc ttgactctct atagatgctt caattgcatt atcgagaata attccaagca
     2281 ccctaataca gtcaaatatt ggaacaggaa cttcaataat agtacttgga cattcgaatt
     2341 tacaacaaat cttattgtta tttgcttggt atagcttcgc tatcaacaaa cttttcaaat
//
LOCUS       DAHHZJ010000228         2395 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.232, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000228 DAHHZJ010000000
VERSION     DAHHZJ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.232"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            316..570
                     /locus_tag="NBL02_002749"
     CDS             316..570
                     /locus_tag="NBL02_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCD2573377.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="NBL02_002750"
     CDS             571..888
                     /locus_tag="NBL02_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCD2573378.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1447..2382
                     /locus_tag="NBL02_002751"
     CDS             1447..2382
                     /locus_tag="NBL02_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573379.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          861 a          339 c          453 g          742 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 ggcaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacatc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 actactttca aaagattatc cgagttaatg cagcagactg ttaaaagtgc tatgcctgaa
     1561 ggatacaagt ttaattttgt tcaatcttca gtatctactg aaagtatcta tttttcaatt
     1621 ctttctgaga gtaggctttt tcattttaga ataagttctc atgtaaagaa aactgatgtt
     1681 gatatatata cgttccgtac aaataattat aaaaatttaa atgatttgag tagaagtatc
     1741 agtgaatatc tcaaggggaa tagctatctc aagattcgat tttcacatat attaatttta
     1801 ctaccatatt tgaatgagaa tttcacccta attcaagtag ataagtcttt cattgcctca
     1861 ggtaatcaag gaaatatgaa tattacaagt aacaaaacgt cacgtgtgct attggcgttg
     1921 atgggttacg gaattcttta tagagatgac caaaatggcc agattctaat aaatcgggct
     1981 gggcgttcat tattaatgaa atatagaaaa tttataccgg aagattttca aaacttacag
     2041 ttagaaaaca tgcgtttaga agatttattt agattagcag aagatgataa gtatagattt
     2101 gaagttttga aaattcaaaa gaatgaacta ttgaataagt tatatcctga gaatgcgata
     2161 aaagcacatt ttggtgatgc taatcatatg tatagttggt tcattcctaa aagatttaga
     2221 gggaaaatca atgctggaga tcaagtgaaa gtactcaatc gaaatagtac agatgtcgta
     2281 atgataacag atatctatgt agaagatgaa gatataatca aatatatgaa gcctgtgata
     2341 aaacttgtta ataaaaataa aggaacccgt ggtgctagtt aattttttcc tagag
//
LOCUS       DAHHZJ010000229         2389 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.233, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000229 DAHHZJ010000000
VERSION     DAHHZJ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.233"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            196..2058
                     /locus_tag="NBL02_002752"
     CDS             196..2058
                     /locus_tag="NBL02_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCD2573380.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2058..>2389
                     /locus_tag="NBL02_002753"
     CDS             2058..>2389
                     /locus_tag="NBL02_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCD2573381.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          859 a          353 c          465 g          712 t
ORIGIN      
        1 aaagcgtttg attatttata ttacgactta gtgcaataaa aaacgctttt taacagtaca
       61 tgtagtcgtc tttataatag ataaactctg taatattagt taaaaagcta taagttgcac
      121 ttagttaagt gcaaacattt catattgtcg ttagtttaga aaacgcttat attagcaatg
      181 taaggagaga tgataatgca gcagctaaca aaaagagaaa ggcagctttt gatttacttg
      241 gtggaaatgt ctgaaccggt aacaatcaaa aagttggctg aaaaagaaaa tgttagtgtt
      301 cggacgataa aatacgatct agatgatata agagagtggc tgaatgagcg gaagcaagtc
      361 ctatatagca aacgtagtca gggaatttgg atgaatatta gtagctctga tcgaatcaaa
      421 ttaaaaagcg aattgatgga tgttaatcgt tttgaaatat ttgcagatca aagtctccga
      481 atcaaacgtt tactaattat gcttttagta acaaaagaag ctattactgg aatgaaaatg
      541 gccaacgcat tagatgtcag caaagatacg atcatgaatg atttagatgt gctggaaaat
      601 aattttctta aggaaggatt gaccttgaat cgtcaggcgc gaaagggttt ttggatcata
      661 ggagaagaac gtctagttcg attaacgatt gaagagattc ttcaaaaaga gttcacggat
      721 tatgacattt ataaattaat gtcattgtta ttgaatggcg gtgagactga atactttgaa
      781 atgtattctg ctactgctac acctatccaa gaggtattta atcaagtaat cattcgaatg
      841 cgacacttac tggaatttga aaatctagag aaactgaatt acgcggaatt actaaatatt
      901 ctgattcgag tgacaattgc taccgttcgc ttacgcaaag aggctacgat tggtcgttat
      961 caattggttg ctgaacaaga attagacaaa aacgatcttt cttatcgcgt catgcagcag
     1021 gtctttgatt attatcagtt gccactgttc gaggatgaat atcattatat ctatagtgat
     1081 acattcgaca cgattcacca gcaagacgtc atgaaactag ttgagaatgt gatttataaa
     1141 gtaagtaaag caatcaacca ttctttttat caagatccac aattgttgac gaatctgtat
     1201 gctcatttat ccatgcgatt atcgagaaag aaaaaatttg tcaatgaata taatcctttt
     1261 aaagaagata tcaagcagaa atacccggaa ttatttgatg cgatcaagca agcagttcga
     1321 caagaaataa gtgaacaaaa tattctgatc aatgattctt tcgtggcata tattgcctta
     1381 cactttttgg tttcctacga aagagaagaa gatctacgaa gcgttagagc cgtctacatt
     1441 tgttcaacag ggttgggtgt gactagtctg atcaagcaaa agatcaatga agaattaaat
     1501 aatatcacta tcgtggcatt tgcttcagta ttgaatgcgc aagaaatgat tgctaccaaa
     1561 gatcctgatt tggtgattag tatttttcca attaatggca tcgatcgtcc tttcatcaaa
     1621 gtccatccat tacccactga acaggacttg aaaaaaattc aacaaataat aaaaaaaata
     1681 cttgcttctt ccaatcagca ggaaaggcat aacttgaatc ttagagaaac gtccatcaat
     1741 aaagatactt tagaagactt tagcaaggaa ttgattttga aagcttatac gatttatgaa
     1801 cgtttacttg tcattttgga agcagaattg atccaagaat atcgagaagc ttttttgctt
     1861 catgtattct taatggttca tcgtatcaca tttgatcaac aatatatggc agaaggtcgt
     1921 caagtagacc agattatttt agacaaagga acactggtag gaaaaatcga agcagttttt
     1981 gcagaaaata atttaggaat caatcagtca gaaatcagtg cattattctc atatataaaa
     2041 gaaggagcgt caagttagtg aaaatgtcag acgaggcttt aaaaattatc gaggaaagtc
     2101 aaaatcggga aaagttaaaa caagtgattg atctaattga agagcgatta gtagaacatg
     2161 ctattgtacc aaccgagttg cagtggacca ttctaattaa tcacttgaat gagatgctga
     2221 aacgttctca aagaaaggaa acgattcccg tagtcgatcg ggatttgttc aaagaagtat
     2281 ccaaagaagc cataactatt tctgatgaaa tagttagaag tatcggtaat ttaaccgatg
     2341 atgaaatata tgttttgtcg attcattttg aaacagcaaa aaataatta
//
LOCUS       DAHHZJ010000230         2368 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.234, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000230 DAHHZJ010000000
VERSION     DAHHZJ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.234"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            204..1817
                     /locus_tag="NBL02_002754"
     CDS             204..1817
                     /locus_tag="NBL02_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCD2573382.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          746 a          419 c          453 g          750 t
ORIGIN      
        1 tcttcagctt ttaaaaaata tacagggtta tccccttcaa agtattatct atccttaaaa
       61 tagaaaaaaa tttttaggtt ttatccacaa attatttctc tatattttct gtctattcaa
      121 aataaacaat aattttccgg cacaattatt tgctgttttt taccataaca tagtacgatc
      181 aacgtagaaa aggagatgtt tttatgaaag ttgttgtcat tggcggtgta gctggcggtc
      241 cttcatttgc cactcgtttc cgtcgattga atgaagcgca cgaaatcatt atctatgaac
      301 gcggagaaaa tatttcttat gcaagttgtg ctttgcctta ttatttagga ggtgtgatca
      361 ctgaccgtga ctcgctgatc gaacgtacac cagaaatatt gaaaacaaaa aacaacatcg
      421 atgtctttac taaacacgaa gtaacagcaa tcgatccttc tactaagcga ttaacagtta
      481 aagacctatc cacaaatgaa gaaataaaaa cagattacga taagttgatc atctcttctg
      541 gtgctagacc agattatccg gatattcccg gagtttttga agcagaaaac ggctttgtcc
      601 tccgtagtgt gacggatgcg gatcgaatca aatcgttcct tgaagaaaaa aatccacaac
      661 atgtcgtcat tcttggtgca ggtgttatgg gtctggaatt agctgagaat ttcaagcatc
      721 gcggcttaga cgtgacttta atcgatcaat tgccacaagt cgctttccct tatgatccag
      781 aaattgctaa tttagtttat gacaaattgc tgaaagaagg attagccgtt catttagaaa
      841 caagagttac tgagatccgt gataaaggtc gagaaatagt attatcagat ggctccgtcc
      901 tttctgctga tatgctaatt tttgctgttg gtgtttctcc gaagaatgaa gtggtgaaag
      961 caaccggcat acaattatca gatacaggtc agatcattgt tgatgaccag ttacaaacca
     1021 atctgccgga catctatgcg attggcgata ttatcgaaac gactagtgta gtgactggtc
     1081 agccgatcca aagtatgctt tccagtgcgg ccaatcgtca aggacatatg ctggcagata
     1141 ttttaaatgg tacgcctatg cgctatcgcg gatatattgg tgcaggtgtc gcaaaaatct
     1201 ttgatcatac agcaagttat gcgggaatga cagaacatga attaaaagca tcaggcataa
     1261 caaattataa aactgttttt atcactccct ttgaccatgc ctatttctat ccagaagcta
     1321 caagattaaa tctaaagctg atttttgatg cagatagcgg tcgtatttta ggtggacaag
     1381 catttggaga aaaaggtgtc gataaacgga tgggagaact ttctgtagca atcaccggaa
     1441 acttgacagt ctttgatttg cctgatttgg agttgcctta ctctccaccg tattctacta
     1501 cccgtgaccc gttgaatata gctggttatg tcgcaatcaa tcaaatgacg aatatcgtag
     1561 aaacgatcaa agcaagtgat atacccgaaa acgatttgaa agaagcgttc tttttagata
     1621 tacgtgaacc taataaagca ccttctggca gtatctcggc aacaaaaaat attccaatga
     1681 atgagttacg agatcgaatc aatgaaatcc caaaagataa aaaagtttac attactttca
     1741 gaagaggatt gaatacttat acttctgccc gaatcttggc aggtttgggt atcaaagcga
     1801 ttttgattga agaataaaaa aacgaccgcg gtgggtagcc gcggtctagg agttattttt
     1861 acttgaggag taaaaatgaa aaagtatata gggttgttgg ttggtatgta tatagaatag
     1921 aagataaatg tgaagaaatt gtgcattatt tcttcacatt tattaggtta tagtttaaga
     1981 aagtttaaac attgcgcaat ttaattattt tagctgtttc ttttcataaa aaaacatgtt
     2041 gtgactctga gacaaatcat agtccatagt gatgtctcta gagtaactac gcctaatatt
     2101 acgaggcatc atattaatct atcagcacta gtacctaaac tagcatccct ttcgaatatt
     2161 ttttcttata taataaagtt tgctttcact cctctaatct caccaaatcg actggcttgc
     2221 gttgtgtgat actaggaact gattggttta gaacgacttt tctattgtaa tcatattttt
     2281 cgccatcctc gttgtcttct aatccttcaa tgaatgatcc ctaatgtgtg tattaaataa
     2341 tttaattttt ttgttcctat ttatttaa
//
LOCUS       DAHHZJ010000231         2351 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.235, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000231 DAHHZJ010000000
VERSION     DAHHZJ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.235"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..544
                     /locus_tag="NBL02_002755"
     CDS             <1..544
                     /locus_tag="NBL02_002755"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCD2573383.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(1017..1589)
                     /locus_tag="NBL02_002756"
     CDS             complement(1017..1589)
                     /locus_tag="NBL02_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCD2573384.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1605..2210)
                     /locus_tag="NBL02_002757"
     CDS             complement(1605..2210)
                     /locus_tag="NBL02_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HCD2573385.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          741 a          468 c          359 g          783 t
ORIGIN      
        1 catttcaaaa gagaatctct taaatcagga cttttctact gaaacgatct gtgagaaatg
       61 ggcagctgac attacgtaca ttcctactaa gaaaaatggt tggtgttact tatcttcaat
      121 catggattta cacacgaaaa gaattattag ttatacattt tcaaaacgaa tgactgtgga
      181 ttgtgtcatt caaacattga ataaagcaaa aatacattat cacattccag aaggaatgat
      241 tctacacact gacttgggca gccaatacac agcaagagaa gtggaacaat ggcttaaaac
      301 caacaaaata aggcattctt atagtagaaa aggaacacct tatgataatg ctggaatcga
      361 atctttccat gcctcattga aaaaagaaga agtctacacg actagctact cagattttga
      421 agaagcaaat cgagcgctat ttagctacat tgaaggattt tataaccgaa atcgaatcca
      481 tagctcgatt cactatctaa ccccacagga atttgaagaa ttagcaaaag aaaaaatggc
      541 ttaaccgtct ctactaaaat gtgtccaaga tattgactca aatccagtaa tatcacaata
      601 ggaatgctgt ttattatatt agcagcagtg ctgattttta tgatagttat ttggttctta
      661 aatgaaagat ctaaagacaa tcaacaccgt agggatatgg agataaaatc aatagagtca
      721 atagattatt tatcgataat aacactaaaa aagccacgtt cctaaacgtg acttacgctg
      781 aacgatatgt gttgtttggt agacgaacaa atcgttcatt ataaatatat gatggtatcc
      841 taatatagta agcgtggcta gcataccttt aattacttta ccttttacag ttaccataca
      901 cattttttga gcagaaatca agtaaataaa aaaatattat atttcacaac atgctaatag
      961 actcatatct agaacgcatt aagtatttac ctgtctggtt cgctgagact tcttttttaa
     1021 ctttcttcaa ttttcttctt tattctaaaa agcgtactgc gactgaaacc ggttatagct
     1081 tcaacttcct tatacgattt acctgaagta agcagttcat atgcgtgcct cttcttcggt
     1141 gtgattgttg ctttcggacg tccttctctg aaattaggat tattctttct tgcaaacatt
     1201 tttccttctt gggtacgttc cacaatcata tcacgttcca tttctgctac actcaacaac
     1261 gtccgtgtca ccattcgccc catggacgta ttttcaatcg ttcctagatt caaaaccttg
     1321 atcgttacgt tatcatcgag taaaggttca atgatctcta atgcttctct cgtgttacga
     1381 gcaaatcgat caagcttcgt aacaactatt tcatctccag gcttcagacc agccaacagc
     1441 tgattgaatt ttggccgatc ccgttttgta cccgtaaatt tttctgaaaa aatattcttc
     1501 ttctctacgc agttctcaag caactgttga atttgttctt ttgtactttg agaagcggtt
     1561 gagacacgtg catatccata tttcgtcatt tggtccacct acttttacaa atcatctatc
     1621 gtatactcag aatacccttc atagaaatag ctgacatcga ttcctttcat atataactcg
     1681 cgatcgtcaa ttttatcagt cagagcattg gaaattagat agcgtatttc caaatcgttt
     1741 atgggacttc gctccattgc agaaagatag tctgctttat cgattaagtt ccaatctaca
     1801 actttttgca attcttcctt taaaatgaga tccagccaaa ttctagtact tcggccattt
     1861 ccttctcgaa atgggtgagc gatattcatc tccacatatt tcttgataat ctcttcaaaa
     1921 gaagtttgtg gcatctgatc aatatgctgt aacgaatgtt ctaaatacat aactggtgca
     1981 aatcgaaaat ttccttttgc aatattgacc gatcggattt ttccggcaaa atcgtaaata
     2041 tccgaaaata gaaattcatg aatttcagat aaacctttaa atgtaccaac ttcgatttca
     2101 tttatttttc cacaatcgta caattctttc gctttttgtt tactgagttt ttcttcaacc
     2161 ttagccaact caacttgatt ggttaatccc aatttattct ctaaaatcat gcgaaatctc
     2221 cttaacttta tgacataagt ttatgaaaca ctctcaaccc cattatatcg cgattttgtt
     2281 tatgtgtcaa aaactattaa gtttctgacc cccgatagat ttttcaagtg tctctatttt
     2341 gcacagtcta g
//
LOCUS       DAHHZJ010000232         2311 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.236, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000232 DAHHZJ010000000
VERSION     DAHHZJ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.236"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            25..702
                     /locus_tag="NBL02_002758"
     CDS             25..702
                     /locus_tag="NBL02_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCD2573386.1"
                     /translation="MNLVPLKNFNAKQMDLFFALCARMKDRGLKKVRFSFEELKELSD
                     YKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEINKTKKFVEVSVNPD
                     LEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYYVVRIEEFRSLMDIP
                     EYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFSSLTSDLPKIS
                     LRNWLEE"
     gene            complement(839..1255)
                     /locus_tag="NBL02_002759"
     CDS             complement(839..1255)
                     /locus_tag="NBL02_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573387.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1258..1542)
                     /locus_tag="NBL02_002760"
     CDS             complement(1258..1542)
                     /locus_tag="NBL02_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573388.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            complement(2242..>2311)
                     /locus_tag="NBL02_002761"
     CDS             complement(2242..>2311)
                     /locus_tag="NBL02_002761"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCD2573389.1"
                     /translation="CSLLVGLLSALFSYWLNNRNKK"
BASE COUNT          748 a          355 c          416 g          792 t
ORIGIN      
        1 ttaacagtca aatatcaaaa tgaattgaat ttagtaccgc tgaaaaattt caatgctaag
       61 cagatggatt tattttttgc attatgcgct cgtatgaaag atcgtggttt gaaaaaagtt
      121 agattttctt tcgaggaatt aaaagagtta agtgattata agatgactgc tacaaaagcc
      181 tttgtcggtg atttagaaaa gctctataaa gatatgctta atcttactta ccgtactgaa
      241 gatgaggatt ctattcgcta ctttgttttg tttactggtt ttgaaatcaa taaaacaaaa
      301 aagtttgttg aagtcagtgt taatccggac ctagaatata ttattaatgg tttaacagct
      361 gaatttagta gatttgaatt atctgctttc acgtcgttgc gttctacgta tgcaaaaact
      421 ctattccgac tgcttatgca gtaccgttct actggttatt atgtagttcg tattgaagag
      481 tttaggagct taatggatat cccagaatac tatcaaatgg gtaacattga tcaaaaagta
      541 ttaaatcctg cattacgaga actaaaaaaa tacttcaatg atcttaaagt cacaaaaata
      601 aaagcaaaaa agggcaataa aatcgccaaa ttagaattta ctttttcaag cctaacgtca
      661 gatttgccaa agatttctct gcgtaactgg ttagaagaat aaataaaaaa gaactcgccc
      721 cccttacatt aagcaagtag acaagtccct tacaaccgac taagattgct agtacattct
      781 agcatattgt atggggcttc taaataaaaa acagatagga ataactccta tctgtttttt
      841 atcgttttag aaaatttgtt gggtcgaaat ttctagaagt gactgtttga ctggtttctt
      901 tttgatgatt ctttggtcga tttcgatttg atggatttag tttagcttcg attttttctt
      961 tcacttcttg atatacttta gaatttgtgc caataagaac gcctgtatct tttagatata
     1021 aatcagcaaa ttctgcgatg atttcagtca tgatggaaat ttcttttccc atagcattta
     1081 gttttgcctg attgctggta gtggtcgtgt tggtatcggg ttcataactc atacataaaa
     1141 agcgaatggc ttccgagtag ttattgatat ctggattttt ttcgatgatg gaatctaaaa
     1201 cgtcaacatg actagggtac aaagtaacat gtttttggac agaagttttt ctcatggtta
     1261 tatcattcct ccgataagca tttcaatgat ttgattattt gtgtagacat actcgcttaa
     1321 tatttgagta ttttctgttt ggatagcaat aaatttatcg agcaataaat cgaattggtc
     1381 ttttttgtaa gtcgtaagtt gacggtcaaa atcactttcg attaaaactt ttagatattt
     1441 atttcgactc catttttctc ctgttttatt cgtgagttct tggcaacgtt tgtctattgt
     1501 tgcaacaaac tttggatcaa tattccgaac taaaatgtcc ataaaaaatc tccttttgca
     1561 attatgtata atttatgtat accatacaaa tactatattt gtatagctgt tttttgccaa
     1621 aatgctatac ataagctacc cacgatggcg aaaaagtgat atcaaatttt tttctgagaa
     1681 acgtgatagg cacagcctat ctacccactt tttttagaaa gtgggtacac gtttccttat
     1741 gctctttaaa attcgggggg gagtgctgca cgtgtaatcg ataaccaaat ttaacagacg
     1801 aaaaattgtt ggaatataaa aatatgacac ccgaaagaaa acgatcgttg gaaaaattaa
     1861 tgcaactagt gaacgaagac ggcggaattt cagacgcagc ttttgacgca tggagcgaat
     1921 ttcatggaga atataattaa gtgggtttaa atggctctaa aatcgcttct aagagttttt
     1981 tagcttggac caatgaaata caccaaaaac taaataaaac gtcttaaatc gaaaaatacg
     2041 agctctcaga gcgactttat ttgtaaatgt tgcatataag aaataattac ggattttaga
     2101 cttttgtagg atatttttga aataaatatt tatttctgtt ataatttgat tgcaaataag
     2161 tgaacacgaa acacacgaaa tccccagaga ggtcggattc tctggggctt tttttgtggg
     2221 ctgagtcgcc ttaattaagt tttacttctt gtttcggtta ttcaaccagt aactgaacaa
     2281 agcgcttaaa agcccaacaa gaagggagca a
//
LOCUS       DAHHZJ010000233         2297 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.237, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000233 DAHHZJ010000000
VERSION     DAHHZJ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.237"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(1015..1305)
                     /locus_tag="NBL02_002762"
     CDS             complement(1015..1305)
                     /locus_tag="NBL02_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573390.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1513..2204)
                     /locus_tag="NBL02_002763"
                     /pseudo
     CDS             complement(1513..2204)
                     /locus_tag="NBL02_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          657 a          434 c          334 g          872 t
ORIGIN      
        1 catacttgca aagtaatact acaattccta acataatcgt agaatttaat aatttccata
       61 gcataaagtg tttagaaaag aacattaaga atagttctat aataattctt gaactccatg
      121 tcttataatg ttctatcgaa gcacccaaat agccaagttc atctaatcta cttgcataaa
      181 ctacgtcatc cgctctcata gtcggcatat acatattaaa gagtaaaaag ataccccaaa
      241 tccctattag taacaagatc agttctttgt tattcgttat ttttctatta tgtttgttca
      301 tttttatact ccttcttata tttcctaata gagtataaca tagctttaag cattacttaa
      361 agataactta aaagttatct tttaaatact taattgttaa ataccttatt tgatgcaaaa
      421 gaaatctacg atagtcaaca aaaaaatttt cattatatcc agaaaaattt ttcttagtta
      481 aggacctatg gattgcagta atgcagaatt cgtcgaataa actgccaaag acatacaatc
      541 atattgcttt taaaattgac gaacaagata tagattcgtt tgtctcaaaa atccaaatgt
      601 taggtttaac tgtcgaacca ggacgttctc gtgtgaaagg cgaggcttct tcaatttact
      661 tttacgacta tgataatcac ctcttcgagt tacataccgg tactctccag gaacgtttaa
      721 aatcctacaa ttccactacc tgagactagt aatgactagt ctctttttat ggcctctgat
      781 ccccccattg agcgaatact aatagggctt cgaattgatc tgttcaatct ttaagcaata
      841 tccctcaaat gagtttggaa agtttagtga ttcatcacaa atacttaaat acatgtagtt
      901 atatatagat tgaggttctt gtgagcgtat gctcgtgtgg gttcaaatcc catcgtctgc
      961 atccatcttt ccaaaacaag cggctaggac atttttgtct cagccgcttg ttttctagtt
     1021 tattctttca tcttgtcgga ccttttttct tgggtataga cttttctcat attctaccac
     1081 aattttgggt ttccctaaca catgatagga tctcacaaca aattgctttc tattattata
     1141 ataacctgta acgactaact tcatcccttc gtctacttca gctaggaagt tcaaactatg
     1201 ggaagcaatc aaacaattga ccccatttaa actgaaacgg atcaacggtc tggcactaac
     1261 ttgcaaagta cggatcttat ctactgttcc atattttttc tccataacct tcacctcaat
     1321 accattatac gaacatacat tccctttgta aagaagaaac tctgttttat ctttttgact
     1381 ttttgtctat tacctttctt aaaatcatat tggcacttcc tgcgaagatg aaggacatgt
     1441 actaaaaaaa agaggcagca gtatagatta ctgttgcttc tttactaatt taggtttact
     1501 tgttaagaaa gattatttac gcaaatgttc ttcaacaatt tgctgagatt tatcagctgt
     1561 atcttccact tcatcttttg ctttttctaa cttttgaata tattgtttca agcttgagtt
     1621 ttcagcagtt gcttcgtcta atttcttttg tgtatctgtt aactgatttc tacatttgtt
     1681 ttttcttgtg tcagtgttgc gatttgttga ttcaaaccat ttacagtatt ttgtaattct
     1741 gaaagttttg cttgtgcttc ttgtagtctt gctgtccaat ctgcattcag atgattgatt
     1801 tcatcatttt tttggttgat ctcatctagt ttgacattga tttcattttg tttgctttgt
     1861 agttgtgcat ccttgctgac taattgactt tctaagtttt gtttttctgc tgccgattga
     1921 tttttttggt tttctaattc atttactttt gtttggtatt cttggacatc tgcctttgaa
     1981 tcttgcaatg tctgttcttt ttgtgctagt cgagcggtaa gatctgtaat cgcttgctct
     2041 ttatctgtca atgtatgatc aagctgtgtc agattctcat tgattgtatc aatatctgtc
     2101 tctccacccc acaaaataat ttcatttgcg taccgcgtgc ctgtgtagcc aattagacat
     2161 agaaatgcaa cgacaatccc accagtaata aatttctttt tcatttgcat agctccttaa
     2221 tccaatactt ttacatgcta aaagtaagtg atctcaaaca caatgtcaag cgtttttaaa
     2281 ttacatatgt aatttta
//
LOCUS       DAHHZJ010000234         2296 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.238, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000234 DAHHZJ010000000
VERSION     DAHHZJ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.238"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(169..468)
                     /locus_tag="NBL02_002764"
     CDS             complement(169..468)
                     /locus_tag="NBL02_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HCD2573391.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            819..1451
                     /locus_tag="NBL02_002765"
     CDS             819..1451
                     /locus_tag="NBL02_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCD2573392.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            1574..2212
                     /locus_tag="NBL02_002766"
     CDS             1574..2212
                     /locus_tag="NBL02_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCD2573393.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT          764 a          364 c          336 g          832 t
ORIGIN      
        1 gtttatacta gctgtttttg tgttaattaa aggataaaat acaatataaa gaaacatctg
       61 tttctttatt tagaataaaa aaatacagac tctgttcaaa taaatgaacc tagcctgcat
      121 tatgagattg actatacttt ttaattcatt gagatgaata actcgttttt ataaatgccc
      181 aaaagtaatc ggtgccgaag ccattcctcg acctaattct ttttcaaatt ttcctattgt
      241 tgatatctcc atataaaacg gtgatactgg tttagacaat tctcccctga ttgctaacgg
      301 acgtaaacta cgttccaaat tatttatgac tttaggataa tactgacctt tttctaagcc
      361 tgtctttgcg tctatcaaaa gtatacgttc cttttctttg atctctggat ttaaaacgag
      421 atcatataat tttagtacca tgttttctat ttcttcactt ttagacatca cgatttcctc
      481 ccaaaaatat atttcttccc actacattct ataaacaaag tatacgctac atctagattt
      541 ttttaaatca ttactggatt gttaaaacct ttcaatacac tgttatggca acgattttta
      601 ttttaaaaac attctaaatt aaatcagtaa aaatttttat tctaagattt ttattagaat
      661 tacgcctaat ttgtgtctct cagggcaatt ctaagaacgt ttcaagagat agcgacacta
      721 tcgttatcga aaaaggcgaa aacctatctc attttctgct aatgtaagtt ggtttagctt
      781 ttgttaatat gactttatga aaatggaggt aattaaatat gatttttcct aaagttaatg
      841 aagcaagctt gaataaagga tccttttttc atatctatga aaacgaagat tttgaactgt
      901 ataatatcct ttcacaaaaa gaggtcatcg aaaaccaaat aatctgccat ccaattattt
      961 acaaatctat tttcaagtat gcattttttg cttccaatga ttttcaaaga atggagatta
     1021 ccgactgtat attcgaaaac tgtgattttt caggctgtat attgacaaaa agcatgttgc
     1081 atcgcgtgaa atttgtcaat tgtaaattaa caggtactaa attgatggaa agttatattg
     1141 ggaacacgtt attcgagaat tgtaaaatgg actatgtaaa tttatctggt tcaaacataa
     1201 aagaaagtac ctttgagcac tctgtcttat caagcgcaga ttttgtagac tgttccctca
     1261 ctaaaataat gttttctact aatgatttag aaaatgctag cttttttgat actgatttaa
     1321 aaaatatcga tttaagtagc aactcctttg aaatgattga agtacgctcg gaacatctaa
     1381 aaaattgtac gcttaatcag ttgcaagcac ttggttttgc tagaaaattt ttacaaataa
     1441 aagtagtata atcaaaatga atatataaga taaattttta gcaatacaaa tatccataaa
     1501 aaaacattgc ctattttaac tcttcatgta tactgactct atcttcaaaa actgaatagt
     1561 aggagttatt atcatgaata acattttagg aaaaaaaata gaacttaaaa aagggatatt
     1621 gggcactatt gtcgttgtac tcggtttgtt agggttgcta tttgcgccat ccattattag
     1681 cattttttct ttaactatca ctggatttat agcatttaca cttgtttttg gtaaaaaaga
     1741 tacgaaaaaa atgttctcga aacctgtcgc tcctgtcaaa aatatagcca aatatttttt
     1801 tatcaatgta gtaatcagtg ccgcagtctc ggtatttttg caacaaattt tgaaatgggg
     1861 tttaacaggc aatccaataa atgaggcttt cagcccgtta ttatttatta ttttgcctat
     1921 catgatactt ggagaagaat tgttctcagt gtattttctc gctatttttt ctagtaagtt
     1981 cagcattcct gtcgctagtt tcttgtcagc aatcatcttt ggatttatcc attacagtac
     2041 ttatgataat ggaaatattt tacacactgt agcacacatc cttttgatac aaggggtggc
     2101 aagattgtta ttcaaccaag ctgccattaa aagcaactcg atcattacaa gttgggcagt
     2161 tcatgtgata tttgatttca cagctatatt gctagtcgtt ttattctcct aaatttttat
     2221 agaaataata catgctctat tgctaagaaa gcatacttta tttcctttaa acaaaaagac
     2281 agatgtatca atggat
//
LOCUS       DAHHZJ010000235         2295 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.239, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000235 DAHHZJ010000000
VERSION     DAHHZJ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.239"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            111..581
                     /locus_tag="NBL02_002767"
     CDS             111..581
                     /locus_tag="NBL02_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCD2573394.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            666..1274
                     /locus_tag="NBL02_002768"
     CDS             666..1274
                     /locus_tag="NBL02_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HCD2573395.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            1408..1608
                     /locus_tag="NBL02_002769"
     CDS             1408..1608
                     /locus_tag="NBL02_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCD2573396.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
BASE COUNT          780 a          320 c          456 g          739 t
ORIGIN      
        1 gcagagacaa aggcaaagga tcaaaaaaaa ttaatttttt taaataaaga tgaaagttat
       61 tgaaatatag agtatgataa ataagaattt ttaataaggt aggttggctc atgaagcagt
      121 atcgacagtt aaaaggatat ttagaagcaa aaggatattg ggagatacta gtctatctct
      181 tttttggtgg tttggctacg attgtaaatt ttgttagttt tgctctcgca cgccaatatt
      241 ttgatttaag tatggcctta tccaattcta tttcgtggtt ttgttctgta ttgtttgctt
      301 ttgtaacgaa caaactttgg gtattccatt caaaatcacc gaattttaca catgcactta
      361 ttgaatgcgg taagttcttc ttctaccgta tcctttctta cggattggac atgggggcaa
      421 tggtattgtt gatcaatgtt atgaacagca atgagtatgt ggcgaaaatc atcactcaaa
      481 ttattgttat ccttgcgaac tatattttca gcaagttgtt tatttttaaa gaaactgagg
      541 tttttgaaga agaagtaggt caaagtgaaa aagacgacta ggcataacaa ttgaagaatt
      601 tccttctctt tgttactctt tgtaatgtag acattattaa gaaaacattg aggaggaatt
      661 tttttatgac ttacacatta ccagatctac cttatgcata tgacgcattg gaaccttata
      721 ttgacgaaga aacgatgcat ctgcatcatg ataagcatca caatacttat gtgacgaatt
      781 taaattcagc aattgagaaa tacccagaat taggcgaaaa aacaatagaa gaattattat
      841 ctgatatgga cgctattcca acagatatca agacagctgt acgtaacaat ggtggcggac
      901 atgctaacca ttcatttttc tgggaaatca tggcaccaaa tgctggtggc gaacctacag
      961 gagaaataaa agaagcgatt aatgaagctt ttggtgattt ttcttctttt aaagaagaat
     1021 tcaaaaaagc agccgctgga cgatttggtt ctggatgggc ttggcttgta atggaaaatg
     1081 gaaaattagc tattacctct actgcaaatc aagattctcc attgatggaa ggaaagacac
     1141 caattctagg tttggacgtt tgggaacatg cttattattt gaaatataaa aatgtccgac
     1201 cagattatat tgcagcattc tggaatgtta ttaattggga tgaagtaaac aaacgtttcg
     1261 aagcagcaaa ataaccattt cctccgtcat atcagtctta aaaataattt ggaaaataaa
     1321 agagcgaaag cttgctagcg ttttctaaat tgggtataat gttcttgcaa ggttacttgc
     1381 aagaactttt ttggaggata tacatttatg gaacacggaa cggttaaatg gttcaacaat
     1441 gaaaaaggat ttgggtttat tacagtagac ggcggagatg acgtatttgt tcatttctca
     1501 gcaattcaag gagacggctt caagagccta gaagaaggtc aagaagtaga attcacgatt
     1561 gttgaaggtg cacgcggacc tcaagctgct gaagtgacta aattataata atttgttgaa
     1621 taaagacacg acttcttaga gagtcgtgtc tttttgtcta tttatgaagt ttttagattt
     1681 tttggctaag taaatgcatt tgttcacttg acatgtccta actaattatc aagcaaggaa
     1741 cattttcttc gttagtaatg gattttaaag aaatttcagt ttttggatgt tgaataaaag
     1801 accatttgtg aaaccactag tatacatact cattaccatg tcaattctat tactttaaaa
     1861 gaaaatcgtt ttactttgaa gaggagcata tctatccatt aaaagatgat ggtaatgcaa
     1921 tcactagttc gagtcatttt aagagccatg cagtgagctg gaaataaaac gaatagaaga
     1981 tgggtttaac aaaaggacca gtcataggaa gaaattgtcg tgaaatcgaa tgataatctg
     2041 aacggtaggc aaagactgtt aaaaagatgg gaaaggtatg tgcgatcatt ttactgcacg
     2101 attatttgga aaataccttg ttgttcgctt tttctggaaa aaatacataa aaaaagataa
     2161 aacgattgcg aactgcgtgt attcggtctt tcgatctttt tcacggtatg gtatgatagt
     2221 taagaatttt gagaaatttt aaaaaataat aatcgttaca caaaaatgtt ttttaaagtt
     2281 ttcttatgat taaaa
//
LOCUS       DAHHZJ010000236         2243 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.240, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000236 DAHHZJ010000000
VERSION     DAHHZJ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.240"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(125..547)
                     /locus_tag="NBL02_002770"
     CDS             complement(125..547)
                     /locus_tag="NBL02_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573397.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            861..1475
                     /locus_tag="NBL02_002771"
     CDS             861..1475
                     /locus_tag="NBL02_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCD2573398.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            complement(1499..1849)
                     /locus_tag="NBL02_002772"
     CDS             complement(1499..1849)
                     /locus_tag="NBL02_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCD2573399.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(1843..2106)
                     /locus_tag="NBL02_002773"
     CDS             complement(1843..2106)
                     /locus_tag="NBL02_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HCD2573400.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
BASE COUNT          734 a          398 c          335 g          776 t
ORIGIN      
        1 gtgtctcctg taaagttcgc tgaacttgta aagctgattc ttctttcggt aaattaattt
       61 tacttagaat atcgctatct tgataaaaat ttggtgtgtt cacaaattct ggcataaaaa
      121 atccctactt tctagttaag gttcctgaat aagtcgcaat ccaattgttt ccagctgatt
      181 tataactttg taaatacaat tttccatagt accaataacc atttcttttt tctttccaat
      241 tccgtgattg aggaggtcta gaaggccaac caactttatc aaaaatcatc gttcctgtca
      301 ctcgtatcgt tttagaagct gctcgagcaa gtaatggaga aactattaaa taattttcag
      361 taacagttaa gtcgttagtt gggttaccaa tggctattcc gttcctatct aaaggagtta
      421 attcttggtt attatctact acagtagttg gttcatttgt actatcagca aatgtaggaa
      481 ttgtagttcc agttacacta attccaatag ttaaaatacc taaagataaa ataaacttat
      541 ttttcattgt ctatcacctc gaaatcaaaa ataagaaaac gttttcttaa taaatcttaa
      601 cagataaatc aagttatata ctagaaaatt cataaaatat atttttaatg tcataatcgt
      661 ttaaattttc ttgattgaca tttatttaca aaaaatattg ccatttaaaa agaaaagtga
      721 cacatactat ttcctccatc attagttttt gtctatataa ggtataccct aactagacac
      781 gaccgcgaca ttgaaaaatc tggttttctc gtgtatgatt agggtgtttt ttaaatagtt
      841 gcagaaagta ggtaaaaatt ttgagagttg catacatacg tgtctcatcc atcgatcaaa
      901 atgaacaacg gcagattgaa gaaatgaaaa aattcggtgc agagagaatt ttcattgaga
      961 aacaatctgg cgcaacgatt actcatcgac ttgtttttca agaagcactg gatttcgtcc
     1021 gagatcaaga tatttttatt gtggaagcta ttgatcgact tggaagaaat tacgacgaga
     1081 tcatcgcttc agtcaattac ttgaagaaga aaaatgtgca gctgatcatc acaagtctac
     1141 cgatcatggc cgaagcaatt ggcaatcctc tcctggataa attcattaaa gatttgatca
     1201 ttcaaatttt ggccatgatc gctgaacaag aacgtaccga atctaaacga cgtcaggcac
     1261 aaggcattaa gattgcaaaa gcgaacggtg tttataaagg acgtcctaag ctatacagtg
     1321 ccaatgcaaa agatccccaa cgtcgtcttg tttataaaaa catcgttgag gatctaaaaa
     1381 aaggtgttgc gatcgctaag attgcaaaag actacaatgt tacgagacaa accgtatata
     1441 gaataaaaaa agacagcatg gtcaatcacg aatagttgat catgctgctg tttctatttt
     1501 atttgcttac tttttgaaaa actgttctaa ctttcatcaa taactcctca actaaactat
     1561 catgcgcttt ttccaaaaaa atacactctc tggcttcata atcaattgtc gttagttgat
     1621 ctaataatac ttttcctgtt gtcttcattt catattctga gtttatattt acatatagcg
     1681 gatagtttct agttgtacta gaaattggag caactgtaac aacgttacta taatccgcaa
     1741 taatatcatg acttaaaaca atataaggtc ttttacctgt ctgttcgtgt ccagatcgag
     1801 gtgctgtatt caataataga atatcgcctt gtctaggttt tctcaccatt tttcattacc
     1861 tactgcttca cctaaatttt gaatcgtcac atgatttctt tcatcagtgt aattttcaaa
     1921 taactgttca attgttaatt cattttctat attagccgct tcgataatta gccgcttctg
     1981 accgtttact tcttcttgta ttatcttcac tttgtcttct gtatgaattc caagatattc
     2041 cataatatac tttggcaatc taaggccatt actatttccc cattttttta taggtaacat
     2101 ttccatagta gacacctctt tctgtatata catagtataa catacataag aaaaaacgta
     2161 tatacttaat tatccttttc tattaaatta gattaacatt ccatgaatcc ataaactatg
     2221 ttaacattta tatgaaaata gtc
//
LOCUS       DAHHZJ010000237         2242 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.241, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000237 DAHHZJ010000000
VERSION     DAHHZJ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.241"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            137..>2242
                     /gene="acm"
                     /locus_tag="NBL02_002774"
     CDS             137..>2242
                     /gene="acm"
                     /locus_tag="NBL02_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HCD2573401.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVII"
BASE COUNT          884 a          345 c          439 g          574 t
ORIGIN      
        1 ttataaatat ttgtaaaata actatcaatt ttttagttaa aagattggaa atgagtgggg
       61 gaggaattga agaaaggatt tttgagagat gatatagtag gttttatttt tagttaaaat
      121 aggggagata gaaaaaatga aaaagtgtag aaatattatt ttatcaatgt tatttattat
      181 tactaatata acatcactga ttcctgttca tgtctatgcg gatgcaggca gagatatcag
      241 cagtaatgtc acttcgttga ccgtggaccc aacgaatatt acagatggag gaaacataaa
      301 agtaaaattc tcgtttgacg aaaaaaaaca aaatatccaa ccaggtgatt atctttggat
      361 caattggcca agtgaaggaa atattcgcgg tgaaggattc caaaaagaaa tcccattaat
      421 gattgaaaat aaaaatgttg gaactttaac ggtcagaaaa gattctgcgc aggttgtgtt
      481 taatgaaaat attaaaaacc tagactcagt agaaggttgg gggcaatttg agatacaggc
      541 cagaaacgta accgataccg gtgaagaaaa tacgggacca ttcaccgtga cgagcggtga
      601 taaaacagct actgtaaatg tgacaaagcc agcttctggt tcttcttcta gcgtttttta
      661 ttataaaaca ggagacatgc tacctgaaga tactaagcac attcgatggt ttttgaatat
      721 taacaataac ggcacttatg ttgaacaacc agttaaaatc agtgatgaaa tccaaagtgg
      781 acaaagatta gatccaagta cgtttgaaat taatcaaata cacttaggtg aacagaaggt
      841 ttatagagga gaagagggaa tccaacaatt tttacaagat ttccccagcg ctacatttaa
      901 tttttcagta actgataatt acattgaaat aactatacca aaaaactttg tcaatcttag
      961 gaaaataatg gtgagttata agacgatcat agaaaaccca gaacaaataa attttgagaa
     1021 tcattcggaa gcttggttta aagaatttaa taaacctgct gttgatggag aaagtttcaa
     1081 tcatacagtt aaaaatattt cagcttctgg tggagtcaac ggtacagtta gaggcgaatt
     1141 gaagattttc aaatatatca atgacacaga gattggtatc ccaaatgtga ctttcgaatt
     1201 aagacgagca gatgaacagc ctattcaagg acagtctagt atcctgttga cttcgaatga
     1261 acagggagaa gctagtatca aaggcctgca agtaggagac tatgttgtaa aagaaaaaga
     1321 agcacccaat tggattgatt ttgatccact aagttcaaat gaactcaaat tctccataaa
     1381 cgaaaatgat acagagggag tcagtttacc tatttataac aagaaaaagg ttacgaacat
     1441 cacagcaact aaaatttgga acggtggaac cactccaaga ccgagtattt attttaaatt
     1501 attcagagca agtacaaaca attgggaacc agttcctgat gcagaaacaa aacgattaga
     1561 caatggaata acttcagtga cttgggaaga tatccaacag tatgatgata gtggaaacga
     1621 atatacgttt aaagtgcaag aagtagatca aaacggaaat gattatgtcc cttcaggtta
     1681 tcaaaaaatt gaaaacgggc tagtcgttac aaatgagaat aaagaagtca tttcagtctc
     1741 aggacaaaaa acatgggaag acaatgagaa ccaagacggc aaacgaccga cgacgatcac
     1801 agtaaatcta ttagccgacg gtcaaccgat ccaacacaaa gaagtaagtg aaaaagatga
     1861 ttggcgctat cgtttcacaa atctgccaaa atacagagac gggcaagaaa tcatctatac
     1921 tgtaacggaa gacaacgtgc ccgaatacag tacgacgatt gacgggtata acatcaaaaa
     1981 cagttataca ccggaaaaaa caaatatatc tataccaggt agccatataa caccgtggaa
     2041 aaattttcca gagaagacag agagagagaa aattagaaat ctattctctc aaaaattgca
     2101 aatatttgct catacaggag aatccagcga acaaagaagc aattatatta gtaagagagc
     2161 tgtacctaaa cagataacaa ataaatcaaa aagcattctt cctaaaacaa gtagtaaaaa
     2221 atctagtttt gctgtaatca tt
//
LOCUS       DAHHZJ010000238         2182 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.242, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000238 DAHHZJ010000000
VERSION     DAHHZJ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.242"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            63..728
                     /locus_tag="NBL02_002775"
     CDS             63..728
                     /locus_tag="NBL02_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCD2573402.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(1012..2097)
                     /gene="xerS"
                     /locus_tag="NBL02_002776"
     CDS             complement(1012..2097)
                     /gene="xerS"
                     /locus_tag="NBL02_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HCD2573403.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT          709 a          329 c          371 g          773 t
ORIGIN      
        1 gaagaaagaa catataaaaa tcggttatct acaatcaaaa aaacttttct tatttcttta
       61 aaatggagtt aagagaaata cgtccaattg acattcaaaa gtggcaaaat aatttattga
      121 gcaaatataa aaattcttat gtaagaaaca tgtatgggat ttttcagatg tcattagatc
      181 gagcagttgt tttgggacta cttgaaaaaa atcctgcaaa agttattgga aatgtgaaga
      241 aaaggaaaac tacagttgat ttttggacaa aagaagaatt tacaaaagtt atttcaactt
      301 tttatattga tgatttgtac caacattttg gttttatttg tatgtggtta ctttttatga
      361 ccgttatgcg tttgggagaa gccactgcat taacttggaa agatattgat tttgaaaata
      421 aaaacttgcg tgtgaataaa acactctatt ataagagagc caaaacctat gagtttgtag
      481 aacccgaaac ggcttctagt attcgtacaa tcgaactaga cgacaataca attacctttt
      541 taaaagcttg gaaggagcgt caagtttcaa taggaggcat gtcgtttgta ctttcttata
      601 atggaacacc tactcagaaa tatacaatcc gtcatattat gagaagacat gcaacgatcg
      661 ttggtgttca tataatccgc atacacggat taaggcatag tcatgcttct ttgcttatat
      721 cacaataaaa agatgcactt cgaattaaag aaaggttaaa acatgctgat gtacaaacga
      781 ctttaggaac ttatggtcat ttgtatctaa attcattgag agagataaca aatgatccca
      841 cagattatat ccaaactaat ttttctaaaa tcaaaccgac acctatttac gataataaaa
      901 aataaatgat aaacaaaagt aggatcacac caaaagaaac acactagaaa aactataata
      961 caggcttttc tagcgtgttg tttcttattt ccgcctattt atacattata ctcataaagc
     1021 atttaatgca tcgatcgtag ccgcattgtc tatatgggta tataaagttg ttgtattggt
     1081 acttgaatgt cccaattgtt gggcagttaa taccaatgaa tttgtctgtt ggtagagtct
     1141 agtagctaat gtatgacgca gtttgtgagg gctgacttga attctaaagc cttttgaata
     1201 ttttgctacc atccgttcga ttgcaatacc ggatatacgc ttggcttctc cttggtaaat
     1261 tgctagaaat aaagcttttt cgttttctgt tactttatat cgttctttcc gaatagctag
     1321 ataattagct aaatattcca ttgcgaatgc tgctatattg acagcatctc ttttatttcc
     1381 ttttctagtt actaccacat tattcttatc taaagatagg tcatctaaat taatatttat
     1441 aacctcggag agtctcaatc cagatcctaa aatcagtgca ttgatcgcca ggtctctttc
     1501 tacatctcgt tgataataga tcaaagctcg tttactcaaa gtatttttat attcattggc
     1561 aatataatct agataatttt gtgtatcctt atctagaaat aattttttct caatggcatt
     1621 ggcacgtgca gctaacgtag ttttatctgt gatggttgct acttgatcca taggattatt
     1681 gtctaagtat ggttttccta gaggattatt ggaacttttc gaaagatatt taaatagaac
     1741 ctttaatgcc gtgatatttc tattgatagt agaagtagat agacctaagt tattattatc
     1801 tttgagctta gtttttgttg tctctttttt tacattttct tttgtgcgac gtttcaaatg
     1861 agatttgtac aagtttaggt cataggcagt taactgtctt atcgaggtga tagaagtatc
     1921 aggaacgata gactcactca gaagccattc ataaaagagc ttataatttt ttagatattc
     1981 taaagtggtt cgaggtgatt gatctttatc ctcacgatct tgaacaaaat cttttatata
     2041 atcgggcata tgaagtatga ttgtatctat tttttgatat atttctgtat ttttcatgtc
     2101 attcatcctt tcaataagta ggggagaaga gtatctctat aattggacta taccaattat
     2161 agaatgtttt gtcaattggt at
//
LOCUS       DAHHZJ010000239         2121 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.243, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000239 DAHHZJ010000000
VERSION     DAHHZJ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.243"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            33..473
                     /locus_tag="NBL02_002777"
     CDS             33..473
                     /locus_tag="NBL02_002777"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCD2573404.1"
                     /translation="MVPRIFGLTPYIIEDKEMSPYYAQGNLIYVRPKNTNEILVGDII
                     TYYENSGKKISTRRVIAVENNHEDFYTKADTKTYIEPGVVKKRNIIGSPIFQIPYIGL
                     VLSKTAFAWIQGLFFTIAFCLTGITAWNTFVELKKKRRRTQKFL"
     gene            601..1284
                     /locus_tag="NBL02_002778"
     CDS             601..1284
                     /locus_tag="NBL02_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HCD2573405.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1593..1666
                     /locus_tag="NBL02_002779"
     tRNA            1593..1666
                     /locus_tag="NBL02_002779"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1627..1629,aa:Arg,seq:tct)
     gene            1825..>2121
                     /locus_tag="NBL02_002780"
     CDS             1825..>2121
                     /locus_tag="NBL02_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HCD2573406.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSD"
BASE COUNT          705 a          332 c          433 g          651 t
ORIGIN      
        1 ttatattttt aattcttatt cttctcgtcg tcttggttcc ccgaattttt ggtttgactc
       61 cttatattat tgaggacaaa gaaatgagtc catattatgc acaaggaaat ctgatatatg
      121 tacgaccgaa aaatacaaat gagattttag tcggagatat tattacatat tatgaaaatt
      181 caggaaaaaa aatctcgacg cgtcgcgtaa tagccgttga aaacaaccac gaagattttt
      241 atacaaaagc tgatacaaag acttatattg agcctggagt agtaaaaaag agaaatatta
      301 tcggtagtcc catctttcag attccatata ttggactagt cctttcaaaa acggcatttg
      361 catggattca ggggttattc tttacgattg ctttttgcct aacaggaatc acagcatgga
      421 atacctttgt agagttgaaa aagaagcgga gacgaacaca gaaatttttg taagaatgtt
      481 aaaaacataa atgtattcca gagatccaac gactcttctg aacatgaaac acgagaaaac
      541 actcatgcag cttgtagatt tagtcagttg ttcgtataat gtgtgaagag gaggagtagt
      601 atgcgcaatg aaatgatgca tcgtcctgta gtcaaagaag aactattgga ttttatgcgt
      661 acccagcaaa aaaaattacc gggtgagcta ggcaaactgg aagaagaagc tcatgtagca
      721 gaagtaccag ttattcccca tgaaacagtt gtatttttga aatttttact tggacagctt
      781 cagcctgaac gaatcttgga gatcggagca gctatcggtt tttcatcaag tgtcatggcc
      841 acgacaatcg gagaaaacgc tcatgttacg acaattgatc gtttcgatgt catgatcgag
      901 aaagctaaaa aaaactatga acgtctagga ctgacagata aagtgacttt gcttgaaggt
      961 caagcagcag atatcttgcc ggagttgtct ggtccgtatg actttatttt tatggatagt
     1021 gcaaaatcaa aatatattga atttttacct gaatgcttac gtttgttaag aaaaggtggc
     1081 gttttgatgg tagatgacat cttccaagga ggaacgatct tgctcccaga tgaagagatt
     1141 ccccgtggca aacgagctat ccatcgtaaa ttaaatgaat ttcttcgtgt tgtgatggat
     1201 catcccgatt tgacatcgac aatcttgcca ttaggtgatg gtgtgatctt aatgacaaag
     1261 gaatcagaaa caatcgagtt gtgatacatg attatggcag cctgtagata atcgattgaa
     1321 tcgagggctc aattcgaact tgatgtaaaa gtttctatat ttgtaagtca attaaaggat
     1381 acaggaaatc taaataaaca ggagtagcat gaagtcaatg tagctcctgt tttcaattga
     1441 taaccttaga acagctgaag gagaatgtct cgttttaaaa gattgattta gtagttgggc
     1501 caaaataatt cattatttgc taattaaata actatttcgt attgacagaa gcaatttaat
     1561 tatctataat tattgatgtt ccgctgattc ttgtcatagt agctcagctg gatagagcat
     1621 ccgccttcta agcggacggt cgggggttcg aatccctcct gtgacgctaa aaaaccgtta
     1681 gaaactgagg tttctaacgg tttttctttg ttttttgtgc caatattgtt tgttttcaaa
     1741 ggcaaataaa ataaaaaaac atccttatcg agtacaatta taaggagcaa aaaaataaaa
     1801 ctcatcagaa aagaggaaga agatatgacg atactatcta gaattttagt gactctagtt
     1861 gcaattgaat ttttttatat catgtatatc gaaacggtga gaactgattc tgacacgaca
     1921 agtagggttt ttaaaatgag taaagaagag ttaagcagaa aatctgttca aacattgttt
     1981 aagaaccaag gagtgtacaa tggactatta ggagtgggac tactttatgg agcttatctt
     2041 tcttccgcat ctaaagaaat cacaagtatg ctattaatat ctattttttt tgttgcatta
     2101 tatggcagtt tgactagcga t
//
LOCUS       DAHHZJ010000240         2082 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.244, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000240 DAHHZJ010000000
VERSION     DAHHZJ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.244"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            69..1988
                     /gene="tet(M)"
                     /locus_tag="NBL02_002781"
     CDS             69..1988
                     /gene="tet(M)"
                     /locus_tag="NBL02_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HCD2573407.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
BASE COUNT          716 a          312 c          423 g          631 t
ORIGIN      
        1 ccagtgataa aagtatttat cactgggatt tttatgccct tttgggtttt tgaatggagg
       61 aaaatcacat gaaaattatt aatattggag ttttagctca tgttgatgca ggaaaaacta
      121 ccttaacaga aagcttatta tataacagtg gagcgattac agaattagga agcgtggaca
      181 aaggtacaac gaggacggat aatacgcttt tagaacgtca gagaggaatt acaattcaga
      241 caggaataac ctcttttcag tgggaaaata cgaaggtgaa catcatagac acgccaggac
      301 atatggattt cttagcagaa gtatatcgtt cattatcagt tttagatggg gcaattctac
      361 tgatttctgc aaaagatggc gtacaagcac aaactcgtat attatttcat gcacttagga
      421 aaatggggat tcccacaatc ttttttatca ataagattga ccaaaatgga attgatttat
      481 caacggttta tcaggatatt aaagagaaac tttctgccga aattgtaatc aaacagaagg
      541 tagaactgta tcctaatatg tgtgtgacga actttaccga atctgaacaa tgggatacgg
      601 taatagaggg aaacgatgac cttttagaga aatatatgtc cggtaaatca ttagaagcat
      661 tggaactcga acaagaggaa agcataagat ttcagaattg ttctctgttc cctctttatc
      721 atggaagtgc aaaaagtaat atagggattg ataaccttat agaagttatt actaataaat
      781 tttattcatc aacacatcga ggtccgtctg aactttgcgg aaatgttttc aaaattgaat
      841 atacaaaaaa aagacaacgt cttgcatata tacgccttta tagtggagta ctacatttac
      901 gagattcggt tagagtatca gaaaaagaaa aaataaaagt tacagaaatg tatacttcaa
      961 taaatggtga attatgtaag attgatagag cttattctgg agaaattgtt attttgcaaa
     1021 atgagttttt gaagttaaat agtgttcttg gagatacaaa actattgcca cagagaaaaa
     1081 agattgaaaa tccgcaccct ctactacaaa caactgttga accgagtaaa cctgaacaga
     1141 gagaaatgtt gcttgatgcc cttttggaaa tctcagatag tgatccgctt ctacgatatt
     1201 acgtggattc tacgacacat gaaattatac tttctttctt agggaaagta caaatggaag
     1261 tgattagtgc actgttgcaa gaaaagtatc atgtggagat agaactaaaa gagcctacag
     1321 tcatttatat ggagagaccg ttaaaaaatg cagaatatac cattcacatc gaagtgccgc
     1381 caaatccttt ctgggcttcc attggtttat ctgtatcacc gcttccgttg ggaagtggaa
     1441 tgcagtatga gagctcggtt tctcttggat acttaaatca atcgtttcaa aatgcagtta
     1501 tggaggggat acgctatggc tgtgaacaag gattgtatgg ttggaatgtg acggactgta
     1561 aaatctgttt taagtatggc ttatactata gccctgttag taccccagca gattttcgga
     1621 tgcttgctcc tattgtattg gaacaagtct taaaaaaagc tggaacagaa ttgttagagc
     1681 catatcttag ttttaaaatt tatgcgccac aggaatatct ttcacgagca tacaacgatg
     1741 ctcctaaata ttgtgcgaac atcgtagaca ctcaattgaa aaataatgag gtcattctta
     1801 gtggagaaat ccctgctcgg tgtattcaag aatatcgtag tgatttaact ttctttacaa
     1861 atggacgtag tgtttgttta acagagttaa aagggtacca tgttactacc ggtgaacctg
     1921 tttgccagcc ccgtcgtcca aatagtcgga tagataaagt acgatatatg ttcaataaaa
     1981 taacttagtg tattttatgt tgttatataa atatggtttc ttgttaaata agatgaaata
     2041 ttttttaata aagatttgaa ttaaagtgta aaggaggaga ta
//
LOCUS       DAHHZJ010000241         2018 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.246, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000241 DAHHZJ010000000
VERSION     DAHHZJ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.246"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(<1..173)
                     /locus_tag="NBL02_002782"
     CDS             complement(<1..173)
                     /locus_tag="NBL02_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD2573408.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINM"
     gene            complement(504..737)
                     /locus_tag="NBL02_002783"
     CDS             complement(504..737)
                     /locus_tag="NBL02_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HCD2573409.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(752..1702)
                     /locus_tag="NBL02_002784"
     CDS             complement(752..1702)
                     /locus_tag="NBL02_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HCD2573410.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(1699..>2018)
                     /locus_tag="NBL02_002785"
     CDS             complement(1699..>2018)
                     /locus_tag="NBL02_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCD2573411.1"
                     /translation="FYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANSL
                     KTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKKE
                     RSL"
BASE COUNT          628 a          379 c          256 g          755 t
ORIGIN      
        1 tacatattaa taagcgcatt aatatgcggc tgaataattt cttcaaataa cgcttcctta
       61 gaagtaaaat gtttataaaa agcacccgta gttacgcctg cggtcaggca cagttgcctt
      121 atcttagttt gctgaaatcc ttctttttca aaatggctta gtccactttc tattaacttt
      181 tggtgagtta taatataatc tctggtttct atgggtactt cctcctaata agatgaatta
      241 actttattat cttacacagg acttgaagtt tcaatcgtta acgtttatta ctgaactcat
      301 tccttagagc taaactaatg agaaagagca gaataactga tacaaaaagc aatcctacaa
      361 gaattaatag actcatctaa atcattcacc ttttctaaaa taatccgaga aagatttaaa
      421 aaaattatat ccagctatta ggataagtcc tatcataatc actccaatag ccattttctt
      481 atcctcccta aaaataaagt cttttactta aaaccttttt tgtaatcgtc aataaatttt
      541 ttccctttat cagttaattg aaatcctata tctaaatagt cctcatatct aagtttagcc
      601 agcatctcac ttacagatgc tggactaaca cttagaactt ttgctaaatc attatttttt
      661 aatttctttt ttctttttga taattcctga attgctagta aataaattag tatagtagtc
      721 gttttaattt gtaccatacc taccctccaa attatttcga aaggccttca gcaattttat
      781 ccaggttcca tttcatcatc gcatagtaac tatcaccatc ttgtcctttt tctgcaattg
      841 aatcagtaaa aatcgttgaa tagataggaa tattggtatc ttttgatact gttttcattg
      901 gtctatcatc cacactgctt tctacgaata aggagggaac ttttgttgtg cgtaattttt
      961 caactaagtg ttttatttga tctggtgttc cttcttcttc agtgttgatt tcccaaatgt
     1021 aagcagaagg cacattatac gctttcgaga aatatttaaa gcatccttca cttgtaacaa
     1081 tcattttttt atcattcgga attgaagcaa atttagattt agcttctttg tctagagaat
     1141 ccaatttttc aatatactta tctagatttt ctttatagaa ttttttatta tcaggatctt
     1201 tttccgctaa ctgtttttca atatttttag cgtaaataat accgttttct aaatttaacc
     1261 aagcatgagg atcttccttc cctttctcac tctgaccctc taagtaaata acatctatgc
     1321 catcacttgc tgcaaaatag tctttgtttt cctctttgtt cgcattttta actaattttg
     1381 taaaccaagc atttcctcca gtttccaagt taacaccgtt ataaaaaatc aaatctgctt
     1441 ttgaagtttt ttgaacatct tcaggcaaag gttcatattc gtggggatct ttcccaatag
     1501 gtacgatact gtgtaaatca attttatctt ttgctatatt ttcggtaata tctgctaaaa
     1561 tcgaattagt aactactact tttaattttt cgttcgaagc cactttatct ttgtctttac
     1621 tatttattga acatgccccg aatataaaaa gagcaatcaa aatccccaga cttcccagta
     1681 ctgctttcca ttttctcatc ataacgatct ctccttcttt cttaaaaatt tttgttttgg
     1741 tgagattaga aaactaatta caaataagaa tgccgctgtt aaaacaatac ttgatcctgc
     1801 tgctatgtta aaactataac caataaacaa tccaagcaat gatgaacttg ctccgactgt
     1861 tgcagaaata aatatcatag ttttcaagct atttgcatat aaatatgctg tagctgctgg
     1921 tgtaattaac attgcaacaa ttaatatagt tcccacactt tgcatagctg ttactgctac
     1981 taaagttaat agaaccatca atagataatg ataaaaac
//
LOCUS       DAHHZJ010000242         1933 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.247, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000242 DAHHZJ010000000
VERSION     DAHHZJ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.247"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(108..1109)
                     /locus_tag="NBL02_002786"
     CDS             complement(108..1109)
                     /locus_tag="NBL02_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HCD2573412.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(1268..1534)
                     /locus_tag="NBL02_002787"
     CDS             complement(1268..1534)
                     /locus_tag="NBL02_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD2573413.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1524..1880)
                     /locus_tag="NBL02_002788"
     CDS             complement(1524..1880)
                     /locus_tag="NBL02_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCD2573414.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          577 a          362 c          229 g          765 t
ORIGIN      
        1 attgcacaag agaaacataa agtcgaccta ttagcatttc ttttcaattc aaacattaaa
       61 aaagtttata cctctgctac taaaaaatga aattatctat ttaaaattta aaacggtaaa
      121 tctgattcat ccaaattgga aaatggatca gcctgtattg gagctttgta tttctccata
      181 ataacagata tctttttttc attttttaaa aaataccttt taaatttctt atagtcctct
      241 gaaatcaaaa tatagcgtga tggatactca tcaaaagaag aatcgtattc ttcctttaaa
      301 attcccgatt tacttaattt ttctagtaca tcaaatactc tatcactcaa gtaactcctt
      361 aaattgttat cactttccca ttgaactact tttctataag ttgaattatc agttgaaaag
      421 atagaatttt catccataaa cgcatatatt agcagcaatc tttcatcatt gtcaaattct
      481 attggatcaa aaatatctaa gttgtcatca aaatctttat cagttccggt aacttttttg
      541 ttaattactg aaggtgtctt gtaagaaata gaatctaaat caaagcgttt tgattctaaa
      601 ttattttgtg ctttctcaat taaataattc actttttttg tgagaaacct tctgatattg
      661 tctttgctat atgttccata aaaaccctta tcttctaaag agtccctgta cttcttaact
      721 tctatatact cttcagccaa aacttgtgga actggttcat ctaagaaaaa aacccctacc
      781 tcgcctttgc tatttttata aaagtaatca atttctgcta tagttcccga tctataatta
      841 tcaactggag aacccaactt atggccaaaa atcgctaata atatatcact attttctaca
      901 atttgtttat taataacact ttgcccagag ctattattta tataagtagg aatcgcattc
      961 gtttcccatc gaattggcat caaaattact tttgtattat ctgtattttc tatattccat
     1021 ctatatatgg tttcttctac ttcattcctc tttgctgcta tatcacctgg tgatgctata
     1081 aatactttta ttagatttat ggttttcatt tattgtacct ttctctttat gtctctttaa
     1141 agtaattttt gtataaaatt tattgacttt tacactatag gacaactttt atctatttag
     1201 tacaaaaagg cccactaaac taattgatta gttaggagcc tattacaggt atattttcaa
     1261 ctcgctttta agcaaattct actaccagct ttttgcctaa tgccctagcg atatcatcaa
     1321 gtttatcgaa agatacgtta tcccctctct caattcgagc aatagtagat tgagttgtat
     1381 gtgctttttg tgcaagctcc gcttgagtta ggcctgcttc ctcccgtgca tgataaagag
     1441 caaccgcagt ttcgagtttt cttccttcac ggtcatatgc ttctgcaaaa tctttgtctt
     1501 tttgcttttc ttcaatcaat tttttaatca tcatttgtga accactcctc tctaagttct
     1561 tttgcatgtt taagttcact tattggtgtt ttctccgttt ttttagtaaa tccatgggta
     1621 atgacatacc ttccattgac cacatggaaa tagatagctc tttggatgtt tgatcctacc
     1681 ttacttctta attcgtacaa attagtgtct aattttttca cccatttcaa ccgttgagct
     1741 actagcatcc cattcttttc tgtttcttct attgtacgta aaagcttcgc actatctttt
     1801 tttggtaatg tttccaacca ttctaaaaat tcatcatgac cattaggtct tttaaatgtt
     1861 tcaaaagata ttttttccat acccaattat atcatatatt gattttatat tatagtatat
     1921 aacataaaaa ttc
//
LOCUS       DAHHZJ010000243         1932 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.248, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000243 DAHHZJ010000000
VERSION     DAHHZJ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.248"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..596
                     /locus_tag="NBL02_002789"
     CDS             <1..596
                     /locus_tag="NBL02_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCD2573415.1"
                     /translation="KQFIHDLFLDAHFSRADIACDILGVPDDFITQYRIVDPVSFKPI
                     YGRSGKLETAYWGSRASERQVRMYNKKLEQERKKQIVPKEIETWWRIEMQLRRGKATD
                     WHAMVRESLDSFASPHYLPGDVKPVDRIMIKGLNQDHSEWAYISRNLKYRLRKLLKEE
                     SQNDELTNHLRETFKESANDLKIELDTWLLGLDVTEK"
     gene            796..999
                     /locus_tag="NBL02_002790"
     CDS             796..999
                     /locus_tag="NBL02_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573416.1"
                     /translation="MSQLGKYYSLSKSNRKVYTLFISDSAKEGFTLGFSISFKKGTKN
                     TSISHNNRELTDSQKINGLSGRK"
     gene            1559..>1932
                     /locus_tag="NBL02_002791"
     CDS             1559..>1932
                     /locus_tag="NBL02_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCD2573417.1"
                     /translation="MSKKGIIVNEWEKLNRISNRRLSVDDKVHPLKLSWSIDRITIVG
                     KLNDTIAYWTQNGVVFVDFEQLMRLNENQGYLKSVGTNGWQLFDQYGEDIAYIEMLKF
                     HPDKGRIDFNPNKIGEFLKGSM"
BASE COUNT          628 a          293 c          454 g          557 t
ORIGIN      
        1 tgaaacaatt cattcatgat ttgtttctgg atgcgcattt ctcacgtgca gatattgctt
       61 gcgatattct cggtgttcct gatgatttta ttacccagta tcgaatcgtt gaccctgtgt
      121 cttttaagcc aatttatggc cgttctggca aattagaaac agcgtactgg ggtagtcgtg
      181 ccagcgagcg acaagtgcgg atgtacaaca aaaagcttga acaggaacga aaaaaacaga
      241 ttgtaccaaa agaaatcgaa acatggtggc gtatagaaat gcagctaaga cgtggcaaag
      301 caacagattg gcatgcgatg gttcgggaaa gtttagatag ctttgcaagt cctcattact
      361 tgcctggaga tgtaaaacct gttgatagaa ttatgataaa agggctgaat caagatcatt
      421 cagagtgggc gtatattagc agaaatttga aatatagatt gcgcaaattg ttgaaagaag
      481 aaagccaaaa cgatgagttg acgaatcatt tacgagagac gtttaaggaa tctgcaaacg
      541 acttgaaaat agaattagac acgtggcttt tgggtttgga cgtgacagag aagtgatacc
      601 gaataagaaa agtcagatga gtgagagtaa atttacgtca atgtttaatg acgtgagttt
      661 acgataagcg attattttgc gtgaagcaaa ataaagcgta aaaggagttg agagcgtgac
      721 acgctttcgg ctcctttttt cgttgtcact tgtgacagcg aataccctcg gagagcattt
      781 ggaattgtga cagccatgtc acaattagga aaatattact ctttgtcaaa gagtaacaga
      841 aaggtttaca ctctttttat tagcgacagc gcgaaggagg ggttcacctt agggtttagt
      901 atttcgttca aaaagggaac aaagaataca agcatttctc ataataatcg tgagttaact
      961 gatagccaaa aaatcaatgg tttgtctggt agaaaataat tagcagattt acaagtggga
     1021 aataaaaagc tgaaagaaga aaattgcatt tcaaactaca ttttctacaa tagctctaaa
     1081 atggcatatt ttgcgttctg agcggtttta gcgtttattt cgtttagtta tcggcacaat
     1141 cggtgaaaca cgttagagga gcgaaaatgg agcctgtaag gcgactggta gcggaacggt
     1201 cgtgttagat tgtgtagccg atagctgaac gaaataaatg ctggcagcgc cattctattt
     1261 cggcaggagg aggctcaagg ggttcgggga atgaaattcc ctgaggtctt tttatttttt
     1321 ctagaggagt gctataatca aagaaaatga gaaaaaagag taaaaaaata gtcgagcagt
     1381 gttgacgcac tctcgacctt tacggaaact acttcaccta gttccgtaac tcaagttcat
     1441 tatacgaata tttttaggtt tcttcaagct agagattttt ggatgtggat ttttagagtt
     1501 acgtgaactc tggaaattct ttccaagaat ctcttttttc gttgaggagg agctattttt
     1561 gagtaaaaaa ggcataatcg taaatgagtg ggaaaaactt aaccgtattt ctaacaggcg
     1621 gttaagtgtt gacgataagg tacaccccct aaaattaagt tggtcaatcg atcgaattac
     1681 aatcgtcgga aaactgaatg ataccatagc ttattggacg caaaatggag ttgtgtttgt
     1741 cgattttgag caattaatgc gtttgaatga aaatcaaggg tatttgaagt cggttggtac
     1801 aaatggttgg caattatttg accaatatgg agaagatatc gcctatatcg aaatgttaaa
     1861 atttcaccct gacaaaggtc gtattgattt taatccaaat aaaattggcg agtttttaaa
     1921 ggggtcgatg aa
//
LOCUS       DAHHZJ010000244         1927 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.249, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000244 DAHHZJ010000000
VERSION     DAHHZJ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.249"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            274..840
                     /gene="amaP"
                     /locus_tag="NBL02_002792"
     CDS             274..840
                     /gene="amaP"
                     /locus_tag="NBL02_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HCD2573418.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            853..1041
                     /locus_tag="NBL02_002793"
     CDS             853..1041
                     /locus_tag="NBL02_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HCD2573419.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1054..1611
                     /locus_tag="NBL02_002794"
     CDS             1054..1611
                     /locus_tag="NBL02_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCD2573420.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1643..1885
                     /locus_tag="NBL02_002795"
     CDS             1643..1885
                     /locus_tag="NBL02_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCD2573421.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          710 a          296 c          395 g          526 t
ORIGIN      
        1 aaataaatag aatcttgtaa taataactga gcataatctt tgcaattttt aatcttgaat
       61 aatatccttt aagtatagcc agcaatcaaa agtaaataga gaataagtaa gcaattgtta
      121 tttcagtatc agactggcaa taaaacgccg ttatgatcca gcaatgtttg gtagcgcttt
      181 atttactgat gctatgctaa ctgttcgcta tctatactgt gattgtcggt agagttattt
      241 acagggtaaa aataagttta taggaggtgg aaaatgaata aggggttaaa aataatcggt
      301 gttatcatga gtttactgct actttcagta tttctagcga ttgcgttgat caatgcgccg
      361 tatattatgc ctgaacaatt ggaacgattt cgcttcttta ctttaacgaa ttactacatg
      421 caacaatata ttttctgggc agctgtagta tttgccgttc ttgctattat catcttgcta
      481 gtcgttcttt tctatccaaa gtcaagagga actttcgtta tgaaacggga agatggcaaa
      541 ctaacaatcg acaaaaaagc gatcgaaggg ttggtacgtt cccatttaca cgaagaagag
      601 ttcattcatt cacctaaagt acggatacgt tcaacgaaaa atcgaatcca tatccatgtc
      661 aacggagact tgaaacgaac ttcttcatta gtagggaaaa caggtgtatt gatgcaagat
      721 atcgaagaag aagtaactaa agtactcgga acaaaagaaa cggtcaaagt ggcagtcaca
      781 tacagcggtt atgaagaaca agaagataca agagactaca agcactctcg agtggaatag
      841 gagggccaaa taatgaaaga actattttca gcatacaagc tccctattat ctttgggggt
      901 cttggactac tgctagctgt tttatggtta acgattggat ttttcaagac attgttgatc
      961 ataatcatga cagcaattgg cgcaggaatc ggtttttatt tgaaacagtc aggaatagtt
     1021 gatcaatttt ttaaatcata ggaggaaaca aagatggaaa acaaaaacat gaatacaaca
     1081 aatggatcag ttttggcaaa tgaaaaagga gaaaaccgca tgatgaatgg tgaattgact
     1141 tatgaagaca aagtcatcca aaaaatcatc ggttatgcat tgaaagatgt agatggtcta
     1201 ttgacattag atggcggatt tttctcaaat atggctgaaa aattagtcaa cacagataac
     1261 gtaacagcag gtatcaatac agaagtaggt aagaaacaag tagctgtgga tctagatgtg
     1321 atcgtagaat acggtaaaga catcgaagat atttacaacc aaatcaaaga attgatcagt
     1381 actgaagtaa acaatatgac acatttagat gttatcgaag tgaatgtgaa tgttgcagac
     1441 atcaaaacac aagaagaata ccaaaaagat caagaaacag tacaagacaa agtaacagaa
     1501 gcagcaaaat ccacaggaca atttgcttca cgccaaacag ataaagcaaa atctgctgta
     1561 ggcaaaggtg cacaaaaagt caaagaaaat aacgaaccac gtgtacaata agaagtaaga
     1621 agtcagttag gaggaattta aaatgggatt tatttgggca ttaatcgtcg gcggtatcat
     1681 tggagcaatt gcaggagcaa tcacgaataa aggatcatca atggggatca tcgcaaacat
     1741 cattgctggt ttagtaggtt cagcaattgg acaagcttta ctaggaactt ggggaccaac
     1801 aattgcagga atggctatca ttccatcatt gatcggtgca atcattttag tattagtcgt
     1861 ttcattcttt gcacgtaaat tataagcaaa ttgaagaaaa acttatgttg aaatgaagaa
     1921 caaattt
//
LOCUS       DAHHZJ010000245         1911 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.250, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000245 DAHHZJ010000000
VERSION     DAHHZJ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.250"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..1087
                     /locus_tag="NBL02_002796"
     CDS             <1..1087
                     /locus_tag="NBL02_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCD2573422.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1305..>1911)
                     /locus_tag="NBL02_002797"
     CDS             complement(1305..>1911)
                     /locus_tag="NBL02_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCD2573423.1"
                     /translation="VEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVRSEELL
                     ALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLKTWKKT
                     QQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFLIEHNK
                     NIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
BASE COUNT          657 a          343 c          331 g          580 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa ccggtggcaa atccatgtaa
     1141 acatggttta ttttttacac tgttttcaaa caaacccttg ctatttcttc ggttcgtcct
     1201 cgtccatttt cgataccgac agctagctta taacccaagg tatatcaaca taaaaaaata
     1261 aaaataggct atgacagcag cgtgacagca aaaaagatgg aaagttaaat atcaaacctc
     1321 gtaatttcat ttgcgagtgc ttgatttgtt tctggatata agtgtccata tttgtccaaa
     1381 gtagttttaa tagaggcatg acctagacgc ttagatattg catagatatt tttattatgt
     1441 tcaattaaaa aggcaacatg cgagtgtctt aatccatgca tatgaattgg ttttactcct
     1501 gcaatttcag ctagtttttt tattctgctt aaaatagttc ttggagatgg cggtaatcca
     1561 tcaaaagaaa aaaccaattc tatatttttc ccaattgatt gttgagtttt tttccatgtt
     1621 tttaaatctt caacggtttg tctgtctaac gaaattatcc gaatacttga tgtatttttt
     1681 gtttcagcaa attcataatc ttgtcttgta cggatgtaaa ttgacttatt tacgctacat
     1741 ctgccattta ttaagtcaaa gtctttccat tgtaacgcta agagctcctc actccttaca
     1801 cctgtaacaa aaataaaacg catcaaacgt ttataaaatt cttcataaaa atccccgtga
     1861 taaacactat tatatacttt cttaaattca tcaactgtcc aaaattctac t
//
LOCUS       DAHHZJ010000246         1878 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.251, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000246 DAHHZJ010000000
VERSION     DAHHZJ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.251"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            72..1112
                     /locus_tag="NBL02_002798"
     CDS             72..1112
                     /locus_tag="NBL02_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCD2573424.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT          716 a          269 c          314 g          579 t
ORIGIN      
        1 tatctttgct attttcgaaa agagtaggta attttacata aataaaacaa cgtgaaagta
       61 gaggatctac aatgactgat tttaaatttt ttaaggcgga tagagtttac aacgaattat
      121 tttatcaatt tccaaaagtc tttattgttt ctgacgaata caaaaaaatg aaagattcaa
      181 ctaagattgc ctatatgctt ttaaaagcaa gattagagat cgcaatcagc aaacggcaaa
      241 tcgatgaaga aggtaatgtt tattttactt atacgacaaa tgaactatgt agagtattaa
      301 actgccaaaa acaaaaagcg atagcaatca aaaaagagtt ggaatccttt ggtttattat
      361 tacaaaagca gatgggattt aacaaacagt tagggaaaaa taatcctaat agactatatc
      421 tagcagaatt aaaagtctca gaaaatgata tctacttact cgaaaaattt gatagagaga
      481 atagggaaaa cgttgataaa tcagagggta tgaaaatcat acccaccctc gacgaaaaat
      541 cagacgctga atcccttggg gctcaagagg gtatgaaaat cataccgtgc caaaacgttg
      601 ataaatcaga gggtatgaaa atcataccag aacttaataa taatatatta gacactaata
      661 gacacaatat agacactgaa aaagaccgcc tacaagatca attgttgtta gacaattttg
      721 agacaattat gacaaacgac agcattgcta cgtttgtccc tgaacgagta ttaaatttga
      781 taaaaacatt ttcttcaagt tacagtgaag ctcaaaaaac cgtccagact attcataatg
      841 caaagaaaaa agctgaaata gaaagtgata tttcgatagt ttttgaagaa ctcgatagtt
      901 attatgtcaa tgcagaacaa gaattataca cgacactgtt aaaagcctat caaaaattaa
      961 aaaccgaaaa agtcgaaaat atccagaacc tgatttttgt ctatgtaaaa aattggttta
     1021 tcgaaaaacc aatagctgct aaagtatcaa gtgaaaaacg tttgaattat gcaagctccc
     1081 caagcactat tacgaaagac tggttagagt gaaactttac tgacaaagtg attgttgctt
     1141 catggagtaa cattaaaatt aaaaattttt tattattcta attatattac gaatattaca
     1201 aaataatagc tattttaata gaatttataa gttaaaatat tatgataaaa atcacaaacg
     1261 tagcctttta cgtcataagt gttaaaagaa ataattaatt tcaaatatag aaaagggaac
     1321 gtatgtttgc atactccttt tttttgagtt ataataatac gcgaagagag ataatttcat
     1381 tatttaaaca agctaaaaat cttgatatta cttcttttca ggcaatacga atattattcg
     1441 cttacatttt ttgtataaat ttaattgtct gctatgcttg tgatgaattg attttcgttt
     1501 tgcacttttg gatgataaat ttggacaatt gaaaatacaa aaaaaagaaa ggcggtatga
     1561 actcggaagg ctggtaacct tcgtcagcgt tcatacacgg cattaagatt tggcccttaa
     1621 tgcgttaacc tttcagtgtt acggtattac ccgcaatgga tttagtataa cagatattca
     1681 aaagtgatac cacttttttt ggctgtgtta aggttaaaaa tacgtgatat tacgatgaat
     1741 ctatgcctaa gcataatgtt ataaaaatgt ccgtgtggct ctttgccgta tgacgaacgc
     1801 tgaaattaga gggaacgcat gtttggatgc gttccctctt ttttgtttgt catgaacgaa
     1861 aatcaattct ctattttt
//
LOCUS       DAHHZJ010000247         1869 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.252, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000247 DAHHZJ010000000
VERSION     DAHHZJ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.252"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(248..469)
                     /locus_tag="NBL02_002799"
     CDS             complement(248..469)
                     /locus_tag="NBL02_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573425.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(567..812)
                     /locus_tag="NBL02_002800"
     CDS             complement(567..812)
                     /locus_tag="NBL02_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573426.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1052..1666
                     /locus_tag="NBL02_002801"
                     /pseudo
     CDS             1052..1666
                     /locus_tag="NBL02_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          627 a          324 c          274 g          644 t
ORIGIN      
        1 tttttactat atacttcttc ccagaacttg tccgtctcct tcaaattatc tctacctctc
       61 gagttcattg ggtggtttgc ccatttatta aacatggaat cacctttctc atactcatat
      121 atttatgtcc tatttcaatt atatttatat atgcttaaat aaatttgtat cctgaaattt
      181 attgtaatat gaagctgtat tttacatata gtaaccaaag agttaataaa ataaaaagag
      241 cgaaccatta tagatcagct cttttttcat tgctgttttt aatcttatct agaatcactt
      301 caaatgtttc aaaggtattt acatctaact gacataacca aagcaagtac ttcttttctt
      361 tgtaagtcca atcaatatta cggtaagagg acattagatt atggtacctt gctctttgtt
      421 caactgaagc caattcccag cgtttttcaa atattttttc ttcttccatt attgatccct
      481 cgcaagacgc tacctcaaac ttaaataatg ggcagcgaaa gtctaattcc ttagatctaa
      541 gtatagcaaa caaatatgaa aaaaacttag aaggtagtaa aagttcttcc gtcttttgct
      601 ttgcttctat tcttaatgtg tttcgttatc ggtgccctac aaagattttc attaattaca
      661 cactcattta ttgttgtgta ttttctgaca tttctctctt gatcaattat tttaattcca
      721 tgggcatcct gatagttcat aggagaggag cggctatcag aaatagttgc acctatctct
      781 tgaacttctt ttacaacttc tggaaaatcc atccatcctg cttcagctgt caaaagtaat
      841 aatctataat atttatcttt ccttttcaat ctaagaaatc gtcaccccat tctttttctt
      901 ctattataaa ctatttaact tttagaattc aaaaaccgtt tgtctattta gagtttaccc
      961 taaatagaca aaaatataac attgaaaaat ttagagttct aacgtatgat tagggtgtat
     1021 tataaacgtt atagaaaagt agggaataaa tttgaaaatt gcttatatta gagtttcatc
     1081 catcgatcaa aatgaacaac gtcaaattga agaaatgaaa aagtttggcg ctgaaaaaat
     1141 ttttatcgaa aaacaatctg gagcgacgat tagtcatcgg ccgattttcc aggaagcttt
     1201 agattttgtc agagagcaag atatttttat tgtagaagcc attgatcgac ttggacgcaa
     1261 ttatgacgag attatcgatt cagtgaatta tctaaagaag aagaatgtgc gacttattat
     1321 caccagttta ccgatcatgg ctgaagcaat tggaaatcct ctcctagata aattcatcaa
     1381 agacttaatc atttaaatct cagccatgat tgctgaacaa gaacgtactg aatcgaaacg
     1441 acgccaggca caaggaatta aaatcgctaa agcaaacggc gtctataaag gtcgtcctaa
     1501 gttatacagt gcagacacca aagatcctca acgtcgcctt gtctatagga gtatcgttca
     1561 ggatcttgag aatggggttg ctatctcaaa gattgcaaca gactacaatg tgactaggca
     1621 aacaatttac cgaataaaaa aagaaatcga ccaactaatt gtatgatttc ttttttgaat
     1681 cacgtaatta ttaattagct ttgtcatcaa tacagacaat cattttagat tcatctagct
     1741 aagttgatta catttatgtt aaaaagaatt tagttaacat aaatataatt gatgcataaa
     1801 tcgtccttat ttaaaggcat ttaacattga caacttagta taatatatct tatgtaaact
     1861 aataagatt
//
LOCUS       DAHHZJ010000248         1853 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.253, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000248 DAHHZJ010000000
VERSION     DAHHZJ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.253"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            117..1199
                     /locus_tag="NBL02_002802"
     CDS             117..1199
                     /locus_tag="NBL02_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013554917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HCD2573427.1"
                     /translation="MSEEGYEQEYVKEAFDTNWVAPLGANVNGFEKELAEYVGASHAA
                     ALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPILYQGAIPVFVDSDYETWNMK
                     PEHLRTALEKYPNTKAVLVVHLYGLSANLDEITKICKEYDVTLIEDAAESLGTTYKGK
                     YTGTFGDYGIYSFNGNKIITTSGGGMLVSNEKEKVEKVRFWATQSREKARHYQHNEIG
                     YNYRMSNIVAGIGRGQLKVLNERIEKKRYIHNFYKKELSKLSNIQFMPENNWNEPNYW
                     LSVITIKGINTNHLIDELDKNNIEARPVWKPMHLQPVFKNYDYIGDDVAETLFDLGVC
                     LPSDTKMTDEDLKYVVSTIKRVINDA"
     gene            1189..1809
                     /locus_tag="NBL02_002803"
     CDS             1189..1809
                     /locus_tag="NBL02_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCD2573428.1"
                     /translation="MMRKKTFYQLFGKRILDILLSGIALIVLSPIILIVGFLVRIKLG
                     SPIIFKQERPGKSEKIFSMYKFRTMTDERDHNGEYLPDEIRLTKFGKILRATSLDELP
                     ELWNILKGDMSIVGPRPLLVEYLPLYSEKQRKRHNVRPGLTGLAQVNGRNAISWEEKF
                     DLDVYYVDKISFFNDLIIIIQTCKKVIKKENINTINNNIPEKFKGS"
BASE COUNT          695 a          248 c          360 g          550 t
ORIGIN      
        1 aagtgtagta ataaaaaata ttaataacaa tactaaaaat gttggcgtac ctaccaaagt
       61 aatagaaaga tgggattaag ttggaaaata aacgaatatt attagcatct cctcatatga
      121 gtgaggaagg atatgaacaa gagtatgtaa aagaagcatt tgatacaaat tgggtggctc
      181 ctttaggtgc gaatgtgaat ggatttgaga aagaattagc agaatacgtg ggagctagtc
      241 atgctgccgc attatcttct ggaacagcag ctatacattt agctttgaaa gcggctgggg
      301 tcggtaaaga tgacatagtg atttgtcagt ccttgacgtt ctcagctact gctaatccaa
      361 ttctctatca gggagctata cctgtatttg ttgatagtga ttacgaaact tggaacatga
      421 aacctgaaca tttgcgaaca gcgctagaaa aatatcctaa cacaaaggct gtattagtag
      481 tacatttgta cggtttatca gctaacttag acgaaattac taaaatatgc aaagaatatg
      541 atgttactct aattgaagat gctgcagaat cattaggaac aacatataaa ggaaaatata
      601 ctggtacatt cggcgactat ggaatttatt cttttaatgg aaataagata atcacaactt
      661 caggtggcgg gatgcttgtg tctaatgaaa aagaaaaggt ggaaaaagtg agattttggg
      721 ctacacaatc tcgtgagaag gcacgccatt atcaacataa tgaaattggc tataattatc
      781 gaatgagcaa tatcgttgca ggtatcggtc gaggacaatt gaaagtttta aatgaaagaa
      841 ttgagaaaaa acgatatatt cataattttt ataaaaaaga attgtctaaa ttgtcaaata
      901 tacaattcat gccagaaaat aattggaatg aaccgaacta ttggttaagc gtcataacaa
      961 taaaggggat aaatacgaat catcttattg atgagttaga taaaaataac atagaagccc
     1021 gcccagtatg gaaacctatg catttacaac ctgttttcaa aaattatgat tatattggcg
     1081 atgatgtagc agaaacttta tttgacttag gagtatgttt accatcagat acaaagatga
     1141 cagatgaaga tttaaaatat gtagtatcta cgattaaaag agtgatcaat gatgcgtaaa
     1201 aaaactttct atcagctttt tgggaaaaga attttagata tcctattgtc aggcattgct
     1261 ctcatcgttt tatcgccgat aatcttaatt gtaggttttt tagtaagaat taaacttggt
     1321 tctccaatta tcttcaaaca agaacgacca ggaaaaagtg aaaaaatttt ttccatgtat
     1381 aaatttcgta caatgactga cgaaagagac cacaatggag aatatttacc tgatgaaatt
     1441 cgactaacta aatttgggaa aatacttaga gcaacaagtc tagatgagct accagaactt
     1501 tggaatattt tgaagggtga tatgtcgatc gtaggacctc gtccattatt agtagagtat
     1561 ttgccactat atagtgagaa gcaacggaaa agacacaacg taagaccagg attaactgga
     1621 ctagctcagg tcaatggaag aaatgcgatt agttgggaag aaaagtttga tttagatgtt
     1681 tattatgttg ataaaattag tttctttaat gatcttatta ttataattca gacatgtaaa
     1741 aaagtaatca agaaagagaa tattaataca atcaataaca atataccaga aaaatttaaa
     1801 gggagttaaa tatagtatgc cattaaatat tttatttctt acgcttttag att
//
LOCUS       DAHHZJ010000249         1851 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.254, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000249 DAHHZJ010000000
VERSION     DAHHZJ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.254"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            119..859
                     /locus_tag="NBL02_002804"
     CDS             119..859
                     /locus_tag="NBL02_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573429.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(1150..1818)
                     /locus_tag="NBL02_002805"
     CDS             complement(1150..1818)
                     /locus_tag="NBL02_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCD2573430.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
BASE COUNT          692 a          321 c          332 g          506 t
ORIGIN      
        1 atatatatat ttatgaaatt catcgctatt ttcttaaaat aatcagcgca ttgctactga
       61 aaacaatgaa tctgtgctat tattgagaaa agttccattt ttgagaagga tggtttgaat
      121 gaaagaaatc agacgaaaac atttccgttt tcctgcgtac gatgatgagt taggcgtaaa
      181 attaaccaac gacaagaagc gagtattatt tgacggacaa gaagatcttt tgactgaaca
      241 gaatcagtcc ccgtttgaac agatgaaaga atttcatttc acgaacacga acaccgagaa
      301 aatccgtcgc aagcgtcgtg caacaaatgg attgaagtca gctccgcgta aggaaaattt
      361 ggaaaagcat aaaggaaaat taccggatta taccggccat taccaatcag aaatcacatc
      421 gacaggacga aaaaaacttt ttggtgattc ctctcgttct gtcagacaaa gtgaaacaag
      481 gacagagtcc aagcatacta cacgaaacgc atctgttgac acgaaggtgc gggagcgttc
      541 ttattttgtt cctaaatatg tacccgcatc gattatccca gaccctagag aaagcaaaat
      601 ttctgaggat catttaatcg aatcgatgaa aaaaacggat gactcttatc tgttgttcga
      661 tacagaaccg acgccgtatc aagaaaaaag aaatggtgag ccgtctgtac gaacattcaa
      721 tcgaacaaaa acgttagagg aaacaaagca gtcagaagaa aaaaaatcga aaaaaagcca
      781 tggtatctta gaacgctcat tacaaggatt gatcgatgat caaggaaact ctttgggaga
      841 aaatagctat tttaaataag tgatatacaa atttatattc ggctacagtt ggaaaggaaa
      901 cgctgcggcg tttccttatt ttatactcta tccgaaagtt tatatcgtat cctcaaattt
      961 ttttgagttt aagcgaattt tttctcccaa taagtaagaa aataatggca aaataaaaaa
     1021 acaaactatt ttcgcagatc gtttgcaaaa atagtttatt tttttataaa gaagatttta
     1081 ctgaataata tcattttagt ttacataatg atatttctgt aaattaaata aagtagtgaa
     1141 aaataagaat cagtgctcta actgttcaag aggatcaagt ggttcaaatg tttctgtagt
     1201 ctgttttgct tccaattggg gcaaaacaga tccaatttcc agatacttca ctttattcaa
     1261 tgtataagat agaatagaac agctaagtaa tgtatacaga aaatgtgtga gatccaaata
     1321 aagcaaggag acaactaaaa taccaaaagt aatcaagaaa agacttttgc caatcttaat
     1381 atgaaaatat ttttcaatgg cttgataaat gatatctgtt ccgcctgtgc ttccgccgaa
     1441 tcgaaagacg agaccgcaac caatgccaga aagaatacct gtcatcaaac tattgatcag
     1501 tagactatct acagcaaaat gtatatgaag gctctcccat aaatgtaaaa agaaaactag
     1561 cgcaaatgtt ccgacgatcg tccggagaaa agcgtgtttt ccaaaaagaa acaatgagat
     1621 aagcaataaa ggtaaattaa aaagcagtga agtgacagaa ggagcgaatc caaaaagatt
     1681 atgcaaaagc aaagcaatac cagtaacccc accatctgcc agcttagatg gaatattgat
     1741 tcctgtcaca gctaatccgt agattcctgc gcctaaaatg attccgcaca attctgtaaa
     1801 taaatttaat tttttcattt ctagcctctt ttcttttgaa aattttcagc a
//
LOCUS       DAHHZJ010000250         1837 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.255, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000250 DAHHZJ010000000
VERSION     DAHHZJ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.255"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            58..1227
                     /locus_tag="NBL02_002806"
     CDS             58..1227
                     /locus_tag="NBL02_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HCD2573431.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            1552..1800
                     /locus_tag="NBL02_002807"
     CDS             1552..1800
                     /locus_tag="NBL02_002807"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573432.1"
                     /translation="MIIEINNEILEKNVYEYLIVTLFIPLSLFTVTKGPPCFSTVAFF
                     ELTVTVNPLYRNTGKTIVLPFEDLNVNVISSLFGEKLL"
BASE COUNT          650 a          315 c          288 g          584 t
ORIGIN      
        1 caagctattt tagctttatt ctttctgtaa aaaagaaagg agactaaata aactattatg
       61 aaaaaaggaa aacatgctaa aaaaagaaaa tattcatttt taatgatcgt tgtcgtgttg
      121 tttagcctta gtgcgttctc tacaggaact attcttggat ggcaatttta caataatgaa
      181 cactctcaga tagttgccca tgctcaatca gctcaaaaaa agttgatact gactaccaat
      241 gaacaacagc tcgttgaaaa aatcgatcaa ctattaagaa aaaatgatta tattggctca
      301 atttacgtaa agaaaaataa tcgcatcatc atggaaaaag gctatggcta tgcgaataaa
      361 ctcacgcaac aaccaaatga tccttctctt tattatcaga ttggttcggt tcaaaaagca
      421 ttgactactc ttttgatttt aaaacaagtc gagcgtgggc acctttcttt agatactaag
      481 ctggctcaat tctatccaca aattgctggc agccagcaaa ttactatccg tgacttgctg
      541 tatatgcgct caggcttgca ccgaactgct tctccaacca taccgatgac tgatgaagaa
      601 gtcgttcaat ttgcgattca gcacttaaaa tttacagatt atcagacctc tcattatgat
      661 tcattgaact ttaccatatt gactggaatt ttgatcaaat taacaaatca accgtatgaa
      721 aaattattga ccgatgatat catcgaacct ttaggattac agcatacaaa attttatgac
      781 caagtcaaaa attcatccaa tcatgcacta tcctatcaaa tgtctaaaga ggatgattac
      841 tttaaaccac tgacagaatc tgaaacagag atccataacg aattagggac tggtaatatc
      901 agtatgtccg tctatgattt aaatacgctg tttacacaag ttttatctgg aaaactagtt
      961 tccaaagact tactcttctc attatggcgt gaaaatgcga atggacatcc atatagtggt
     1021 ggggtatata gcggagataa ttatatcctg tcgcaaggaa atatcgactc ttttgataca
     1081 tctgctatat ttagcaaaga ttcacaggat gctatcatca tggaaagcaa tataaaagct
     1141 gacaaaacta tcaaactgcc tactgctgat ctaagaaacc aaatctatga cctgatggaa
     1201 ggaactacta agttggcaaa tagctgatat gatgaaaaga tagcaaatac tacttctttg
     1261 aatagcattt gcttaaaaaa agtactcgac taaacgtagg aaagagtgtg ggacaaatct
     1321 aaaaatcaat ttttgctaca gactttaatt ccaaataaac agaacagaaa gaggtgttct
     1381 tctttctgtt ctgtccaaat tattctaaga tttctcttta ccaacattat ttgacaaagg
     1441 gcctattaaa tctaaaatag tatctttaga ttgctaaaat atgtgctagc aaatcattca
     1501 tggcttgttc ttatttttaa ttttatgcaa taaaaaagaa gaacaattag cgtgattata
     1561 gaaataaata atgagatttt agaaaaaaat gtgtatgaat acctgattgt tactttattt
     1621 attccacttt cactttttac tgtaacaaaa ggacctcctt gttttagtac tgtagcattt
     1681 tttgaattaa cagtaaccgt taatccttta taccggaata caggtaaaac tattgttttg
     1741 ccttttgaag atttaaacgt aaatgtaatt tcatctttat ttggagaaaa gctactataa
     1801 ataacttgac catcttctgt aacttgatgg tttttca
//
LOCUS       DAHHZJ010000251         1761 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.256, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000251 DAHHZJ010000000
VERSION     DAHHZJ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.256"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(<1..165)
                     /locus_tag="NBL02_002808"
     CDS             complement(<1..165)
                     /locus_tag="NBL02_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD2573433.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQF"
     gene            complement(159..560)
                     /locus_tag="NBL02_002809"
     CDS             complement(159..560)
                     /locus_tag="NBL02_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCD2573434.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(747..>1761)
                     /gene="uidA"
                     /locus_tag="NBL02_002810"
     CDS             complement(747..>1761)
                     /gene="uidA"
                     /locus_tag="NBL02_002810"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCD2573435.1"
                     /translation="VIDTYTEEFGIRSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGR
                     GLNEAVNIKDFNLMKWIGANSFRTSHYPYSEEIMRLADREGIVVIDEVPAVGLHLNFM
                     ATLLDDNVEKHNTWKEIRTENSHKTVIKELIERDKNHPSVVMWSIANEPDSDSEGAKE
                     YFEPLVELTKELDPQKRPVTIVTYLKSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAH
                     AKYLLKKELDGWMERCPDTPIMFTEYGADTVAGFHDTVPTMFTEEYQVAYYEANHEVI
                     DTCENFVGEQVWNFADFATSQGILRVQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFS
                     YKK"
BASE COUNT          551 a          352 c          230 g          628 t
ORIGIN      
        1 gaattgacct acctcgttaa atccatttgt caaacataac ttagccatat aagaatgata
       61 atgtttgagt atcatcttga tcgaaattgg atctccttct agagcagata agataatgga
      121 tagtggaact aataggtact gccgcttttt agatttatct accataggtc ataccccatt
      181 ttcaattgtt caactgcctt ctttcgttga taccagatac cacttgtgct catattgaga
      241 attttaccga tttcagcatc attaaaacct gcaaaataat aaagtaggat aatctttctt
      301 cttatctccg ataactgatc aattgctgct gataactttt gatctgaaat taatatttcc
      361 attccaaata agaaatagac atcttgttca tcaaaatgag tttgggaata cctgccttca
      421 tttgaatctg ccaatggttc ctcattatcc tttatgttag cgtttcgttt tcgaatagac
      481 ttagcttcgt ttcgtaatac ttttttacag taggcatcaa attggtgttg tatagatatg
      541 tcattgtaat gttgttgcat gataatatgc tcctttttcc ccctttccac taatacaaca
      601 atcagaaagc cccttttttt cgaaaaaact agaaaaaaaa cataaaaata tttatcattc
      661 tttaaacaga aaatctttct ataaaaaaca aaaaaaaacg attaggctag gctaatcgtt
      721 ttttgaacat tcttaaaaaa tccaaattat tttttataac tgaaattggg tattgatgac
      781 catcgtttct gtagatagta agcaatcatt ttaggttttc tatctctagt aaagattcct
      841 ttcttatttc cttgcactct taaaatccct tgacttgttg caaaatctgc aaaattccac
      901 acttgttcac caacaaaatt ttcacatgta tctattactt cgtgatttgc ttcgtaataa
      961 gccacttgat actcttctgt aaacattgtt ggtactgtat catgaaatcc agctactgta
     1021 tctgctccat attctgtaaa cattattggc gtatcaggac atctttccat ccatccatct
     1081 agctcttttt tcaacagata ttttgcatgt gccagatccc ctcccgaaac ataccaacca
     1141 taataacgat ttaaacataa aacatcgaca atatctccta ccttacaaac atctggcgtt
     1201 gattttagat aagtaacgat tgttacaggt cttttctgcg gatcaagttc tttagttaat
     1261 tcaactaaag gttcaaaata ttctttagca ccttcggaat ccgaatctgg ttcatttgca
     1321 atagaccaca ttacaacact tgggtggttc ttgtcgcgtt ctatcaattc cttgatcacc
     1381 gttttatgag aattttctgt tcgtatctct ttccacgtat tatgtttttc cacattatca
     1441 tctaataaag tggccatgaa atttaaatgt aaccctactg caggtacttc atcaataact
     1501 acaatacctt ctcgatctgc taatcgcata atttcttctg agtaaggata atgtgatgtt
     1561 ctaaaagaat tagcacctat ccatttcatt aaattaaaat ctttaatatt tacagcctca
     1621 ttcaaaccac gtccattaac atacgaatcc tcatgcttac caaatccttt aaaataaaaa
     1681 ggtttattat taatcaaaaa tttgccattg gtaacctgaa cactgcgaat accaaattct
     1741 tctgtatagg tatcgatcac t
//
LOCUS       DAHHZJ010000252         1743 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.257, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000252 DAHHZJ010000000
VERSION     DAHHZJ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.257"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(<1..182)
                     /locus_tag="NBL02_002811"
     CDS             complement(<1..182)
                     /locus_tag="NBL02_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCD2573436.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="NBL02_002812"
     CDS             517..951
                     /locus_tag="NBL02_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCD2573437.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="NBL02_002813"
     CDS             974..1294
                     /locus_tag="NBL02_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573438.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="NBL02_002814"
     CDS             1435..1626
                     /locus_tag="NBL02_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573439.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
BASE COUNT          665 a          260 c          364 g          454 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaa
//
LOCUS       DAHHZJ010000253         1714 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.258, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000253 DAHHZJ010000000
VERSION     DAHHZJ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.258"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            192..788
                     /locus_tag="NBL02_002815"
     CDS             192..788
                     /locus_tag="NBL02_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCD2573440.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(846..1328)
                     /locus_tag="NBL02_002816"
     CDS             complement(846..1328)
                     /locus_tag="NBL02_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573441.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1434..1631)
                     /locus_tag="NBL02_002817"
     CDS             complement(1434..1631)
                     /locus_tag="NBL02_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573442.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
BASE COUNT          575 a          349 c          263 g          527 t
ORIGIN      
        1 tcctaaaaca caacaatgaa agcgataact aagaaaatcc ttacataacg atttttctta
       61 ttaaaattgt tttttgaact tcgcttattt tttgtgagca tatctgttta cttacttttt
      121 gtaaggtgct atacttcatt tcgtaatcaa ttaaaaaaat gcaaaacgca aatcaaagga
      181 gaaattttat tatgacaact tttactgaca ctttgaaaaa ccgccgttca atctatcatc
      241 taggacgtaa tgtttcactt tctaatgaag aattaacaac tttgatcaaa gaagcaatta
      301 aagaaagccc aactgctttc aatgctcaat caacacgcgc agttatcctt ttcggagatg
      361 cacatgaaaa actttgggaa atcactgaag aagcacttcg cccattgact ccagcagaag
      421 ctttcccaaa tacacaaaac aaattagctg gtttcaaaaa tggttatggt acagtattat
      481 tcttcaaaga tactgacgtg gtaaaaggtc tacaagaaca atttgaatta tatgcagaca
      541 acttcccaga ttggtcagaa caatcaaacg gaatcgcaac agctaatact tgggtagcat
      601 tagttgataa aggattgggc gcaaacttgc aacattacaa cccagttatt gacgaagcag
      661 ttgcaaaaga atggaatatc ccttcaaact ggaaactacg ttcgcaatta gtattcggtt
      721 caccagaaac accggctggg gaaaaagaat acatgaacga tgccgatcgc ttccgcgtat
      781 ttggataatt ttatttgaaa taaaaaacag aggttgtgac aaacatcttg tcgcaacctc
      841 tgtttttatt ctggatcaat cataatatca atctctgcct catcgtttaa gcgttgggac
      901 aaaaatggaa ctaattcttc taatgatgca gaaaatttca gcatcggtcc tcgccatccg
      961 tttcggaaaa aattcatttt tacgatttta gaaccttttg gctgatataa tacaatttta
     1021 ttgatctcag aaaaaggaac accttttttg tcaaagctat tcaaaacaag tccttcttct
     1081 gtgattcctc tactgtccaa caaaaaacta ataaaaatca ttgctgacaa gatccctttt
     1141 gccaattcat ctgctgtagg atgactgatg agtgtatacc aaatgactag tgacactaag
     1201 aagggtatca ccaagtagac ccatcgattt cgcacagtcc gataaataat ttcttttcga
     1261 accagccaaa gaaataaaaa catcacaaca acgaataaaa acaatagaac cgtaaagaat
     1321 gttaccatga caactcgcct cgctttttct tctccagaaa ataggaaaag aatactgatt
     1381 taccttctca aagaaattgg acttgcagca cgccgcacgt ccaattgttt tctttattca
     1441 aatgtatcat attttgcaac aaccatgctt gctttttcat ctaataattc ttggtcctct
     1501 gcagcacatt gggcaacttt gaccacggcg cagtttgtca tttttgatac aacttcacca
     1561 atgaccgttt ttgtaaaacc aattggtttg catgcatatt gttctcctaa attaatcatt
     1621 tcttccgtca tcaaaatcct cctaaactgc tggaactttt ctccacatta ccactgctat
     1681 ttctttggag atttaactca ttgattttta ttta
//
LOCUS       DAHHZJ010000254         1710 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.259, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000254 DAHHZJ010000000
VERSION     DAHHZJ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.259"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            15..>1710
                     /locus_tag="NBL02_002818"
     CDS             15..>1710
                     /locus_tag="NBL02_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573443.1"
                     /translation="MKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLE
                     KPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTL
                     TVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMY
                     KQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYY
                     WSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLAC
                     IGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISY
                     NLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLM
                     HTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLP
                     EELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYD
                     TNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT          557 a          327 c          296 g          530 t
ORIGIN      
        1 cggacagcaa tcaaatgaaa gaaataaaag attcgtgggt tttctataca aaagatgatc
       61 ctgattttaa gtttgacagc aagtacactc gtacgattcc taccgtggat aaagacgaaa
      121 cattcattat ggaaacgaca cttgaaaaac cgttggacga agcaaattta ctaatcaaag
      181 gaaaccatca atggattact gtatatctag gcgaaaagat tttataccaa cggaaagatt
      241 atcaggaaga aagcaatccc ggactctcat tggctgtgat agatttacca gcaaattata
      301 tcggaaaaaa attgaccctt acagtctcat ctccttatca gaactatgct ggactgccac
      361 cgaaagttta tctaggaaca actggaccat tgattagttt tatttattca caatcagtgc
      421 cgcaagtatt gacgatgata atcgctgttt ttatcgctat agggaccttt ttctatacga
      481 tctacttgat gtataaacaa aaacgtcttg aatactcact tattctctta agttgctttg
      541 ctttggcatt aggatttgaa gctgtatcag aagatatttt aagtggtatt ctttttgaac
      601 cattcgttca ttctttttta tctcatttat ttattatctt aacgtcagct ttcatcatct
      661 gctactattg gagccgaatg ttttactata aaaaatggta cggaattttt tgtacgttcc
      721 aacttttgat ttttatcggc gttctgcttt atgcaacttt tactcctgca gaacttcctg
      781 aattaatgcc gattgctaat attgctagtg ttataagcac tttagtcact tcacttgcat
      841 gcattggcga agcatataag aataaccgtt tttatgtagc ttgtacacct tggatcgtct
      901 tggtagccat tatccattgc ttgatttaca tccaaacggc attaggtatc tatcaaacaa
      961 cgatcaattg gtctacgatt ttatatatta tcattctaat cgttattatc agctacaatc
     1021 taatcgatca catcttaaat accgacaaag atcggagaca agtagatttc ttaaaaatca
     1081 aaaatgattt gttggagcaa cattatgagc agcttcgaca gcatatccaa gaagtaaata
     1141 cacttcgcct ggaatttgtc caagagatgg agaatctgca agatttaatg catacggacc
     1201 aaacgaaagc taaaaactat gtggaaaaga tattagaaga agcaaaaaat ttcgaaatgc
     1261 tgtgttcttt ttgcaaccac caggtgacaa atcttatctt agcgcgttac cagaagttag
     1321 ctgccaaacg gaatattcag ataaccttcc aagcagatct tcccgaagaa cttcctatca
     1381 acgatgacga tctggctcaa cttttgatcc acgctttaga gcattccttt agagaaactc
     1441 atgccatcga aaatccactg tatcgaaaaa tttatttgtc gatacacgaa caagaggaac
     1501 atttcgtcat ttgttgcgaa catagtgcac attacgatac aaacctattt agtcgaggca
     1561 tcacagaaga attagatgat caggaacgtt ttgacttaat gatgatgaaa gacgtagcgg
     1621 atcgttacac aggaacacta acgcaggaaa aagatacttt cattgatcgg atcactgttc
     1681 aactctctcg aaattacccg aatcctgtat
//
LOCUS       DAHHZJ010000255         1664 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.260, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000255 DAHHZJ010000000
VERSION     DAHHZJ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.260"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            131..505
                     /locus_tag="NBL02_002819"
     CDS             131..505
                     /locus_tag="NBL02_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCD2573444.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="NBL02_002820"
     CDS             complement(619..1248)
                     /locus_tag="NBL02_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCD2573445.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="NBL02_002821"
     tRNA            complement(1382..1454)
                     /locus_tag="NBL02_002821"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DAHHZJ010000256         1658 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.261, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000256 DAHHZJ010000000
VERSION     DAHHZJ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.261"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..1412
                     /locus_tag="NBL02_002822"
     CDS             <1..1412
                     /locus_tag="NBL02_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCD2573446.1"
                     /translation="PTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTN
                     TPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNV
                     TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNA
                     DKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTY
                     PDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYT
                     WKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTG
                     KTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNV
                     VVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGL
                     ILLGTLGLLGYKNRKKENE"
BASE COUNT          658 a          251 c          355 g          394 t
ORIGIN      
        1 aaccaacagt agaaggcgaa aaagtagaag ttgggggcac agtagatcta acagataatg
       61 ttactaactt gccagcgtta ccagaaggaa caacagtaac agatgttact cccggcggta
      121 caattgatac caacacacca ggtaattacg aaggtgtcat tgaagtaacg tatccagacg
      181 gtacgaaaga tacggtgaaa gttccagtag aagtgacaga caatcgttct gacgccgata
      241 aatatgaacc aacagtagaa ggcgaaaaag tagaaattgg aggcacagta gatttaacag
      301 ataatgttac taacttgcca gcgttaccag aaggaacaac agtaacagat gttactcccg
      361 gcggtacaat tgataccaac acaccaggca attatgaagg tgtcattgaa gtaacgtatc
      421 cagacggtac gaaagatacg gtgaaagttc cagtagaagt gacagacaat cgttctaatg
      481 cagataaata caatcctgaa ggacaaaaag tgactacaga cttaaataaa gagccagatg
      541 catctgaggg aattaagaac aaagaagatt taccaaaagg aactacttat gcttggaaag
      601 agaaagtaga tgttagcaca gctggaaata aaaaaggtac tattgtagtg acatatccag
      661 atggatcatc tgatgaagtt gaagtagacg ttacagtgac agatactcgt tccgatgcag
      721 ataaatataa tcctgaagga caaaaagtga ctacagactt aaataaagag ccagatgcat
      781 ctgagggaat taagaacaaa gaagatctac caaaaggaac tacttatact tggaaagaaa
      841 aagtagatgt tagtacagct ggaaacaaaa aaggtactgt agtagtaaca tatccagatg
      901 gatctaaaga agaagtagaa gttactattt ctgtagtaga taaaaaagca ccgaataaac
      961 ctcaagttga tcctattaca gagggtgacc aaattgttac tggtaaaact gaaccaaatg
     1021 cagaggtgac agttacatta ccagacggaa gtcaacacca tgggacagct gataaaaatg
     1081 gtaattttac agttaaagtt cctaaattag aggcagggac aaaagtgata gtaactgcaa
     1141 ctgatgaatc tggtaatact agtgaaccta ctaatgtagt tgtttcttcc aacaaaaaag
     1201 acagtggaaa aaacggtagt aaaggtagta agactgacaa tcaaggtagc aacagcaatc
     1261 aagacaaaaa tagaggcaaa agccagtcct caaaagtttt tcctaagacg ggagagagtg
     1321 acagtaatat ctttactatt agcggtggtt taatactgtt aggaacgtta ggattattag
     1381 ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa tttcttaagt cttctctgag
     1441 aagaggaaga atctttttct tcctcttttt gcttttttat gatgggaaat aagtgttagt
     1501 taaagatgaa tatacttttt atagaaagga aaagaatgta attaagtgga aaaaatagta
     1561 attattggtt caaaccattc agggctagcc gcagctcgat ttctaaactt aagcgattca
     1621 aattatgaag ttattctaat cgataaaaaa tcagaaat
//
LOCUS       DAHHZJ010000257         1649 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.262, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000257 DAHHZJ010000000
VERSION     DAHHZJ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.262"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(86..463)
                     /locus_tag="NBL02_002823"
     CDS             complement(86..463)
                     /locus_tag="NBL02_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573447.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(763..1644)
                     /gene="galU"
                     /locus_tag="NBL02_002824"
     CDS             complement(763..1644)
                     /gene="galU"
                     /locus_tag="NBL02_002824"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCD2573448.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          454 a          370 c          243 g          582 t
ORIGIN      
        1 aagctttttt ccaccaatta aataaatctc tcatctcaat agtttatcca ttattttatc
       61 tttttttgcg tatacctact aaatattacc gtcttcttct cgttcttcta ctttgttcga
      121 gagaatctgt actagaaaaa gtagttgtat gtctaccttt tctgcctatc cgagactttc
      181 ttttcatata tgggatataa atgattagat acagtacagc aaaaaataaa gcaaatacgc
      241 aagcttccca actacctaac gccaattcaa ccatcaaggt agcaacccca acggatgctg
      301 cataccagat aaaaatcaga aacgtttctt tttttctgac catgcgaaac atgataaata
      361 gactaataag caatagtggc aacacaaata tccaattgct tccttccatc agccacttga
      421 gtatatcaag cagcgactgt gcaacagcga ctaattttcc catagtgaac acacaccttt
      481 ttttataatt aaaaaattcg cctttttatt ctactatgaa tcttctggct tggctttcat
      541 ttctcttatg taatcatttt cttttataca acttaaaaaa aaattaaacc attagcataa
      601 tattttctga atctattcac gaaagtcttt ttctatcttt cgttttccac gactcaactg
      661 atctagagtc aaaaaactaa aacgacagtt gcgcaaatga cgcacaagct gccgctttaa
      721 taacaaatag cctttcattc tcagcaggcc acttatttcc agttaacttt ctgttcttag
      781 ctttttactc aactggataa tatatgcact taatgaagtc ccaatttccg aatgttgaag
      841 tccatattca atatttgtct tcatatatcc taacttatcg cctacgtcgt atctttttcc
      901 tttaaactct ctcgcataga ccgcctctgt ctggttaaga gtctgaagtg catccgttag
      961 ctgtatctcg tttcctgctc ctggttcttg agtttctaat atctcaaata ttttaggagt
     1021 cagtagataa cgtccgataa tggctaaatt acttggtgct tttcccattt caggtttttc
     1081 tacaaaccct tttactctgc aacgaccctt ttcgctatct gttttaggat caataattcc
     1141 gtatttagat gtttcatttt ctggtacacg catgacggca acagcggaag cttgtgtttc
     1201 ttcataatcc atcatcaatt gttgagtcaa tgggatatca tctttcatca aatcatcccc
     1261 cagcatgacg acaaacggtt catttcctat aaatgctttt gcttgaagca cggcatcccc
     1321 cagtcctctt ggatgagatt gacggatgaa gtgaagattg acttgagtcg tttcttcaac
     1381 taaagcgagt aattctgttt tttccttttc ccgcaaattg ttttccaatt cgatatttgc
     1441 atcaaagtga tcttcaatgg gtcttttttc ttttcccgtt acgatcaaga tgtcttcgat
     1501 tcccgaatcc aatgcttctt ccacaataaa ctggatcgtt ggtttatcca cgattggaag
     1561 catttctttt gccatggctt ttgttgccgg caaaaaacgt gttcctagcc cagcggcagg
     1621 aatgactgct tttttgacct tcacttgct
//
LOCUS       DAHHZJ010000258         1599 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.263, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000258 DAHHZJ010000000
VERSION     DAHHZJ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.263"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..679
                     /locus_tag="NBL02_002825"
     CDS             <1..679
                     /locus_tag="NBL02_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HCD2573449.1"
                     /translation="EIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDTIR
                     SEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYY
                     TKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMHIT
                     IYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLV
                     DNFNNIS"
     gene            696..1130
                     /locus_tag="NBL02_002826"
     CDS             696..1130
                     /locus_tag="NBL02_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573450.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
BASE COUNT          600 a          182 c          277 g          540 t
ORIGIN      
        1 agaaatatat ctgtttcttt atgaaaataa aatactggaa tatgttaaaa atggagatat
       61 tcgaaattta gaaaacatgg tttttaatct gagtaatgga ataattccaa gtgttagtgg
      121 agatacgatt cggtctgaaa aaaattattc tatcattgtt tttgaaaagt tagcacaaac
      181 gtctataaca ttaggaatgg atataataga agcgtatcaa agtagagatg ctttgataca
      241 agaaaacgaa ttagctgtat cgttacctga ggtcttaaaa gtgagggatt ctgggattgt
      301 ttattatact aaggaaatcg gaaaaactaa gatagagcat ctttctccct taatttcttc
      361 tgtcgttcaa ttcatcggct taaatatcta taaaagaatt actgtaaagg aaatagctaa
      421 ttatttttct gtaagtgaaa ctaaattaag agaaagcttt aagaatgaaa tgcatattac
      481 gatttataat tatatctcta agcgaaaaat ttctacggct aaaataatgt tgaaatcaaa
      541 ccatactatc agtgaagttt ctttaggttt aggattttca gattcttcac acttttcgag
      601 agtattcaaa aagtacgcag gtgtatctcc taagcaatat caattaggtt tagtggacaa
      661 tttcaacaac attagctgag aggttttatt ttattatgag actatatcat ttacgtagtt
      721 tggaggagtt gaggaaattg gattttaaca aaaaattttt ttgggaaatt tctgatttga
      781 gttgtttcgt agaatctgag cgtctaagta atggtaataa tagtgtgtta aatgaatatg
      841 acaaagtagt aattaataaa gtaaaaagtt tatgtgaaga agctagagaa tctatcagtc
      901 atattaatgg tagcaatttt aaacaaacat tatttaatct gattaagcta gatgctaaaa
      961 tatcaagtta tttattcttt ttgatacatg atgaatttat ggattatcaa aaattggatc
     1021 agttaattga aagagaaagc tgggaagatt attacgtggg tctaactttt tcagaaaagt
     1081 taaacaacta caagttaata gattataagt atttgagtga aagcaattaa ttattcatgt
     1141 agagtactag acactttaaa tatggacaat tcaattataa agaaaaagaa tcaataattt
     1201 aatgaggctg tgacaaatgt cattttttgt ttcgctaaaa acgagtgaaa aaatgttgat
     1261 aactccaatc attcgtttaa gattctagga atcattcaga aatgctgtga aaccgatact
     1321 ttataattat aaagaaggtg atttcaaatt ccgaagaagg aatttgaagt caccttttta
     1381 cttatgtcac agtctcattc agtaatgcgg gataaggctc cttattccgt tatttgaata
     1441 ctctgaagtt taatctaaga aagggtaata ttttgaaaac atgcttcatc gcatagaaat
     1501 gaacttaatt tcagaaagaa tacatacata tctttttata tgttttcagc tgctgaatgg
     1561 ttaaaaagca caaatatccg aaaaaaacat gaagaaatg
//
LOCUS       DAHHZJ010000259         1584 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.264, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000259 DAHHZJ010000000
VERSION     DAHHZJ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.264"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..233
                     /locus_tag="NBL02_002827"
     CDS             <1..233
                     /locus_tag="NBL02_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCD2573451.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
     gene            392..>1584
                     /locus_tag="NBL02_002828"
     CDS             392..>1584
                     /locus_tag="NBL02_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCD2573452.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLN"
BASE COUNT          510 a          305 c          293 g          476 t
ORIGIN      
        1 tattaaaaaa tcttcctgaa acacttgatg cacaacttcg cacgaaactt caaaaccttt
       61 taacgtatga agaaggtatt tacaatgcta tgatttaccc ttattctaat ggcaaaatag
      121 aggccaagat tccccatatc aagacactga aacgattatc ttatggattt aaatcatttg
      181 agaacatgaa aatcaggatt tttttgatca atcaattgat tcaagtgaaa taacaaaaaa
      241 atctgagaca gaaggtcagt ccttctatcg gctctatact ttttgatgtt ggtgaacttc
      301 accaacatct tttttgtata aaaaaatata gaaaagacac cctgaaatcc tttagaatta
      361 atttggctac caattcatag gaggattcaa gatgtctcat actcagctta tcaaagatac
      421 cctaaatatt ctagacctaa atatctattt cgaggaaaat tgtttaacta aagaaaaata
      481 caaagggcaa atttgcttga tttacaaagg aactcttctg tacaagccag aagagtgttc
      541 ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg gggacgacaa ccgttcggtt
      601 gttactgaat gatgtgtctg agtatcgcac ttatctggag ctgaagaaac aacgatttaa
      661 atgtaagtct tgccaaagaa cattcgtagc ggatacctct gttgctgaaa aacactgttt
      721 cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta aaagagaata cttccatgac
      781 ggaaatcgct cgtcagaaaa atatttccac ttcttcggtc tatcgtgtca tgaagcgatt
      841 ctaccgccca ttgaatcctt tcaaacagac tttgccaaag gtcttatgct ttgatgaatt
      901 caaatcggtt cgaagtgttg ccagtgcaat gagttttatc atgatggacg gacaaagcca
      961 tgcattattg gatattgtcg aaaatcgacg cttgccttat ttagaacgct atttttctcg
     1021 gttttcatta gcgactcgtg aagccgttca attgattgtt attgatatgt atgcgcctta
     1081 tgtttcgttg gtgaaaaagc ttttcccgaa tgctcagttg atcatcgatc gtttccacat
     1141 cgtccaacat attggcagaa ctttcttgaa tcatcgagtg aaagaaacca cggtacgact
     1201 taaaggaaat tcccactctc aacgagggtt agggaaaaaa ttgaagcgct attggaaact
     1261 cctacaaaaa gaagaagcaa agttaaacta tgaaaaacgg gtttggcgtg ccagttttaa
     1321 agattatttg actgaaagtg agattgccga tcgactgtta gctgcttgtc ccaacttacg
     1381 ccaaggctat cagttatacc aagacattct ttatgcggta aagaaaagag atcagtcact
     1441 atttgaggat tgtttaacta gagacattag cggtctgcca gaaacctata caaccacttt
     1501 acgaacgttt aaaaagtttc tgccacagat ccaaaatgcg cttcactaca gctattcaaa
     1561 cggaccattg gaatgcttaa acaa
//
LOCUS       DAHHZJ010000260         1583 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.265, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000260 DAHHZJ010000000
VERSION     DAHHZJ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.265"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            25..>1583
                     /locus_tag="NBL02_002829"
     CDS             25..>1583
                     /locus_tag="NBL02_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2573453.1"
                     /translation="MVPAFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREY
                     ENLYNSTYKESAHLRVQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIP
                     KAGGMALFFPFISVMLLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRE
                     NSEEFQETIELQQEINSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSY
                     VSLAVVILVGVHLLLTGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRI
                     RAMKETSIQEGSDSPLKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGS
                     GKTSILKLVSRLYDYDEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIR
                     IGNSQASDEEVKKAARLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLK
                     NAPILILDEITASLDAENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGK
                     LVDQGTHNELYR"
BASE COUNT          515 a          231 c          311 g          526 t
ORIGIN      
        1 agtttttttg tatactgcat taatatggta ccagcattca ttataatgat gctgatagac
       61 gaattagttt tagaaaatac aaagccccgt tggctttatt ttgccgtatc gtttgttact
      121 ttgcttttca tgtattggtt gttagatagg gagtatgaaa acttatataa tagtacttat
      181 aaagaaagtg cgcatttaag agtgcaaatt gcagacgatt tgtcaaattt accattatcc
      241 tatttttcaa aacataattt atcagattta tctcaaacta tcatgtctga cgttgaaggt
      301 attgagcatg cgatgagtca tgcaatacct aaagccggcg gtatggctct gtttttccct
      361 tttatttcag tgatgctttt ggttggtaat gtcaaaatgg gattagctgt tattttgcca
      421 acgttattta gttttgtctt aatcttgtta tcaaagaaat cccaaacgaa agccaatact
      481 aaatattacg atactttgag agaaaactcg gaagaatttc aagaaactat agaattgcag
      541 caagagatta atagctttaa tctatctaaa aaagttcaag acagactttt caaaaaaatg
      601 gaagagagtg aaaggattca tttaaaggta gaattaagta ctttttcagt catggcctta
      661 tcctctattt tctcatatgt tagtttagca gtagtcattc tagtaggtgt tcacttactg
      721 ttaacgggtg aagtgactat actctacgtc gttggttact tactagccgc aataaaaata
      781 aaggattcct tcgattctat gaaagaagca gtgcttgaaa tattttattt agctcctaaa
      841 atacagcgaa ttagagctat gaaggagacc agtatccaag agggatcaga tagtcctctg
      901 aaatcttttg acattgagtt gcgagatgtc tctttctcat atgataacaa taccccaatt
      961 ttagatcata tctcttttac ggctaaacaa ggagaagtga ctgcgttagt aggtgctagt
     1021 ggttccggga aaactagtat cttgaaatta gtgtctagac tgtatgatta tgatgaaggc
     1081 tgtattttaa ttgatgggta tgatattaaa cgtgtatctc cagcttcttt gtttagcaaa
     1141 atcgctattg tttttcaaga ggtaactttg tttaatacaa gtattttgga aaatatacgt
     1201 attggaaata gtcaagcgtc agatgaggaa gtgaaaaaag cagctcgttt agctaactgc
     1261 gaagacttta ttgaaaaact acctgacggt tatcatacat tggtcggaga aaacggaagt
     1321 tcactttctg gaggagagcg acaacgccta tccatagcac gggctttttt gaaaaatgcg
     1381 ccaattttaa ttttagatga aattactgcc agtttagatg cggaaaatga aaaacgtatt
     1441 caggaaagtt taaatcgttt aatacaagat aagacagtac taattatatc gcatcgcttg
     1501 aagtctattg aaaaggtaaa taaaattgta gtgatggatc aaggaaaact agttgatcaa
     1561 ggtactcata atgagttgta tcg
//
LOCUS       DAHHZJ010000261         1575 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.266, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000261 DAHHZJ010000000
VERSION     DAHHZJ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.266"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(215..550)
                     /locus_tag="NBL02_002830"
                     /pseudo
     CDS             complement(215..550)
                     /locus_tag="NBL02_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(647..1099)
                     /locus_tag="NBL02_002831"
     CDS             complement(647..1099)
                     /locus_tag="NBL02_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573454.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            complement(1106..>1575)
                     /locus_tag="NBL02_002832"
     CDS             complement(1106..>1575)
                     /locus_tag="NBL02_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HCD2573455.1"
                     /translation="VYATEKVNTVDLEIKNKLVDELTFYFDTVGFLDEHGVYHIKDFG
                     AIRERISQDNEQGRAARVFYEHIIVPRMEKVDWKRYAGCVAINSLPGGGVAWAIGTQG
                     DFIEALKAFNFEKAADLLLKIAEKQLGKAAAKELAKVNIVAGIAAAAASCALE"
BASE COUNT          561 a          296 c          169 g          549 t
ORIGIN      
        1 agaataaata gccttttgca tagctgctta acttataaga aattgcaatt tacttcttca
       61 aagtgaaaac gaatatgagt aaaataaaat tattcttctt acagataatt aaaaatagcg
      121 cgtaacaaaa atcggcattc aatcacataa ttaggattga tgccgattac ttatattcta
      181 cgtatagttt tagtattctt attttttatc accagtaacc aattgagaac agccctaaag
      241 cagaatagtc ttttatctca aaacgcttaa tttcaggctt aggaatatga ataatctttt
      301 gagtatattc tttctctttt aatcttactg aagaaatacc actgcctatc ttttttacaa
      361 atatttcatt tggtgtacag actaataaaa caatatttgt aatcaatcct tgcactaaag
      421 aattaaaagg aagactattt aaaacagggt gtgcataact ataacatgtg aagtagttat
      481 tttgaacttt aacttttaaa aactttaaaa ataatcttat ttgttcttct aaacaatcat
      541 ctatactcat ataatcactc cactaattaa tctccccaaa aagacacagc attaagagat
      601 ataccaacac aacagtgcaa ataactctac atcttccttc gcaaccttat ttcaataaac
      661 cataaaaatt attttttaat agaaactgaa aattaaaact agaaaaagta gttttaccga
      721 atgtaagagc atcatcacca tcaatataga aatacattct tttccagtta catttaattt
      781 ttaaacaaaa ttctccccaa aaaggaactt tttctagtga aaacttggta atctctttgt
      841 aattaattct tgttaaagaa taattaaaca cttcattttt acttgttatt ttttcattga
      901 ctataactag ttcatctggt aaaaaaatta accaacaaac atcgacaaac agtaaatgat
      961 ataatccatt tccacctcca agcatattca atactggtga acatgcctta gaaacacgaa
     1021 tatagttatg gtttaaatag atattttctt taaacattgc ttgtttaaaa gccatatgct
     1081 cttgttctaa acttttcata aacttttact ctaatgcaca gctagcagca gctgctgcaa
     1141 ttcctgctac aatatttacc ttagctaact ctttagctgc agcttttcct aattgttttt
     1201 ctgcaatttt taataataaa tccgcagctt tttcaaaatt aaaagctttt aatgcttcaa
     1261 taaaatctcc ctgcgtacct atagcccaag ccactcctcc tccaggaaga gaattaatgg
     1321 ctacacatcc agcatatcgt ttccaatcaa ctttctccat tctaggaaca ataatatgtt
     1381 cataaaagac tcttgctgct cttccttgtt cgttatcttg actgattcgt tcacgaatag
     1441 caccaaaatc ctttatatgg tatacaccat gttcgtctaa aaaaccaact gtatcaaaat
     1501 aaaaagttaa ttcatctact aatttattct ttatttctaa atcaactgta ttcacttttt
     1561 ctgtggcata cacac
//
LOCUS       DAHHZJ010000262         1568 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.267, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000262 DAHHZJ010000000
VERSION     DAHHZJ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.267"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            76..1404
                     /locus_tag="NBL02_002833"
     CDS             76..1404
                     /locus_tag="NBL02_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573456.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            1415..>1568
                     /locus_tag="NBL02_002834"
     CDS             1415..>1568
                     /locus_tag="NBL02_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573457.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKE"
BASE COUNT          530 a          257 c          298 g          483 t
ORIGIN      
        1 taagacataa agttttagtt tttctttatc gttggttata attaaattat aaaaagattt
       61 taaagaggtg agaaaatgat tggcgaagat aaattattga tgactatcca gctcacagac
      121 ttaaatgaag aagaaaaaaa acaggaaata agcgattggg ctattttgac acgtctgttg
      181 atccagccaa catttatccg ctctttcaca caacaagcgg atccgtatga tcttcgaaag
      241 cttcaagaaa aaataatgct agcatcggta agagacgaaa gttggttagt tgtaggaaat
      301 gacgagaacg aatgtagttt tcgcttggaa gataatcaat tattgataaa aaatgtattg
      361 tctatctctg ttttcaaaga aaagcaattt ttgattcgag acttcattca gaaaaaaatg
      421 attgctcatg gggtgttcgc ctatatgcga gcttatagcg agtttatcta tcacaataca
      481 aaacagatct ctcaacggct attgtttgag aaaaaggatg aaatcgaaca tttacctaaa
      541 atgaaacaac aaaacgggga agtagtagtc gattgcaacc aatttccagg atacgatctt
      601 ttttaccaag gtttatgttt cacttcgtgt tgggaaatgt attatagtcg ctattatcat
      661 caaatcatcc ctaaaccgat ttttctcgac atccaacaag tagaaaaagt gaaggaatta
      721 gaaaacgaag tcatatgtat ccagctttat cgagacccct ttaactggca aaagccaaac
      781 aatcaaatgt ttcaatccta ttttagggac cagcttgggt ttgatcatct tgcttgggac
      841 aatggtgtcg gtttattgaa accgccattc gttgaataca tttacactga ccatacgatc
      901 caatctgtcc agtatcagaa tgcacaaatg caacctgttc ctaaaaagaa tgcttcattt
      961 tttgtaacaa aaagttatga tattcaacaa ggagattata aagaaaggcg tgttcgcgga
     1021 acactaaatg ctcaggcgta ttttccttgg gttgacgaga atcgctcgcg catgatgtgc
     1081 tataaagtta tcgatccgac tgttgctttg gataatggaa tcgaagctta ttgttattat
     1141 attagggaat atctagaagt agaggtaaca gatgaaaaat atcaagagta tcttttatcc
     1201 ttaagaatct atgttccttc agaatccctg tcagaattac cgttaaagga aatcaaacat
     1261 agactctctg atgttacctt caaacgaata aagaaaaagc ggcgaagtat ccaattcgat
     1321 gtcaagaaag gtgagcagca tctgcgtgtg caatttttag attaccgaga attagaacaa
     1381 ttgattactt tacaaaaaat atgaggtgaa gaaaatggat aaacaactct attatcgaat
     1441 aatggttccg gcatcattat tatccgtctg tctctttttt gttcctaatt ttcctattcg
     1501 atcagtcatc ggttttttgg tcatgtttgg cgggatggga ctttattatt atcttcttca
     1561 taaggaaa
//
LOCUS       DAHHZJ010000263         1560 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.268, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000263 DAHHZJ010000000
VERSION     DAHHZJ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.268"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            48..1514
                     /locus_tag="NBL02_002835"
     CDS             48..1514
                     /locus_tag="NBL02_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HCD2573458.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          433 a          290 c          329 g          508 t
ORIGIN      
        1 aagatgtata atattttctg aaaatttaaa gaattggggt atagattatg gaaaaacagc
       61 aagacaaggg cttcttcggg cagccgaagg gattgtcaac tttgttcttc actgagatgt
      121 gggaacgatt tagttattac ggtatgcgtg cattacttat ctattatatg tacgatacag
      181 ttgccaacgg gggacttgga ttaccacgag caacagcatt agctatcatg tcgatctacg
      241 gatctttgat ctacatgtct agtatcgtcg gcggatgggt atctgaccga gtgttaggag
      301 caaaacgcac tgtattcttc ggtggagttt tcattatgct tggccacatc gtcttggctt
      361 taccatttgg tgtaagtaca ttgtttgttt cgatggcatt gattattatc ggaaccgcct
      421 ttttgaaacc aaatgtttct ggcatggtcg gacatcttta ttcaaagact gacttgcgaa
      481 gagatgcagg attttcgatt ttttacatgg gtgtcaactt aggggcatta ttagcaccac
      541 taatcgttgg tacaatcggt caagaatata actatcattt agggttctct ttagctgcta
      601 ttgggatgtt ctttggatta ttacaataca tcatccaagg acgcaagaca ttagacggag
      661 tgggtacaac accgccgaat cctttgacaa ctgaagaacg taaaggattc atccgtaact
      721 tgatcatcgc acttgttgtt atcgctgcag tattcggcgg ggcacaacta actggtcatc
      781 tgacaattga cttttttgtc aatacaatca gtgtattggg gattatctta ccgttgtatt
      841 actttatcaa aatgctgact tcaaaagatg taactgctga agaaaaacca agagtatggg
      901 cttatatccc attattcctt gcagcaatca ttttctggtc attacaagaa caaggttcat
      961 ctatcttagc tttatttgct agtgaacgta cacaatctac gctatttggt gtaacgatcc
     1021 aagcaagctg gtatcaatca ttgaatccaa ttttcatctt tattctgaca ccagtattcg
     1081 taacgatgtg gacgaaactt ggtgaacgtc aaccatctac agtagtgaaa ttctctcttg
     1141 gacttttatt tgcaggatta tcgtacttgc tattgatgtt gccgggtatg ctttatggta
     1201 caagtggaaa agtaagtcca ttgtggttgt tgggaagttt cttcatcgtg gttattgctg
     1261 aattgtgtct ttcaccagtt ggtttgtctg tgacaacaaa attagcacca cgagcatttg
     1321 aatcacaaac aatcgctatt tggttcttag ctgatgcttc ttcacaagca atcaatgcac
     1381 aaattgcacg cttctacaca ccgggaacag aatctgctta cttcggtatt atcggtgcag
     1441 tagcaatcgt cggtggtatc ttgctattta ctgtgaaaga accaatcaaa aaactaatgg
     1501 gctctattca ttaatcaatg aacataaaaa caggaattgc ttatttgcag cagttcctgt
//
LOCUS       DAHHZJ010000264         1559 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.269, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000264 DAHHZJ010000000
VERSION     DAHHZJ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.269"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..449
                     /locus_tag="NBL02_002836"
     CDS             <1..449
                     /locus_tag="NBL02_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HCD2573459.1"
                     /translation="LVTAARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGL
                     VERFNENVNDYNELLEENKSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFK
                     GFVDKVKDKTAQFQEKHDLEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            694..>1559
                     /locus_tag="NBL02_002837"
     CDS             694..>1559
                     /locus_tag="NBL02_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HCD2573460.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GLLKEIHKKLNL"
BASE COUNT          613 a          184 c          317 g          445 t
ORIGIN      
        1 acttagtgac tgctgcgaga gataacgata ggttaaaaca gcatgttaga aatctcatga
       61 gtactgatat ggcgagagaa tataaaaaat taagtaaaga acatgggcaa gttaaagaaa
      121 aatatagtgg tcttgtagag cgatttaatg aaaatgtaaa tgattataat gagttgcttg
      181 aagaaaacaa gtctttaaag tctaaaataa gcgatttaaa gcgtgatgtg agtttaatct
      241 atgaaagcac taaggaattc cttaaggaac gtacagacgg cttaaaagcc tttaaaaacg
      301 tttttaaggg gtttgtagac aaggtaaagg ataaaacagc acaattccaa gaaaaacacg
      361 atttagaacc taaaaagaac gaatttgaac taactcataa ccgagaggta aaaaaagaac
      421 gaagtcgaga tcagggaatg agtttataaa ataaaaaaag cacctgaaaa ggtgtctttt
      481 tttgatggtt ttgaacttgt tctttcttat cttgatacat atagaaataa cgtcattttt
      541 attttagttg ctgaaaggtg cgttgaagtg ttggtatgta tgtgttttaa agtattgaaa
      601 acccttaaaa ttggttgcac agaaaaaccc catctgttaa agttataagt gaccaaacaa
      661 ataactaaat agatgggggt ttcttttaat attatgtgtc ctaatagtag catttattca
      721 gatgaaaaat caagggtttt agtggacaag acaaaaagtg gaaaagtgag accatggaga
      781 gaaaagaaaa tcgctaatgt tgattacttt gaacttctgc atattcttga atttaaaaag
      841 gctgaaagag taaaagattg tgctgaaata ttagagtata aacaaaatcg tgaaacaggc
      901 gaaagaaagt tgtatcgagt gtggttttgt aaatccaggc tttgtccaat gtgcaactgg
      961 aggagagcaa tgaaacatgg cattcagtca caaaaggttg ttgctgaagt tattaaacaa
     1021 aagccaacag ttcgttggtt gtttctcaca ttaacagtta aaaatgttta tgatggcgaa
     1081 gaattaaata agagtttgtc agatatggct caaggatttc gccgaatgat gcaatataaa
     1141 aaaattaata aaaatcttgt tggttttatg cgtgcaacgg aagtgacaat aaataataaa
     1201 gataattctt ataatcagca catgcatgta ttggtatgtg tggaaccaac ttattttaag
     1261 aatacagaaa actacgtgaa tcaaaaacaa tggattcaat tttggaaaaa ggcaatgaaa
     1321 ttagactatg atccaaatgt aaaagttcaa atgattcgac cgaaaaataa atataaatcg
     1381 gatatacaat cggcaattga cgaaactgca aaatatcctg taaaggatac ggattttatg
     1441 accgatgatg aagaaaagaa tttgaaacgt ttgtctgatt tggaggaagg tttacaccgt
     1501 aaaaggttaa tctcctatgg tggtttgtta aaagaaatac ataaaaaatt aaaccttga
//
LOCUS       DAHHZJ010000265         1526 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.270, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000265 DAHHZJ010000000
VERSION     DAHHZJ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.270"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            18..>1526
                     /locus_tag="NBL02_002838"
     CDS             18..>1526
                     /locus_tag="NBL02_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HCD2573461.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLD"
BASE COUNT          594 a          210 c          269 g          453 t
ORIGIN      
        1 ttcaggaggt gaagataatg ggcatcgaat ttagcgaacg gcaaatgaaa atactatact
       61 ttttgattga aaatgagcac acaacttaca aagaattatc agaacaatta tatgtatcta
      121 cgaaaacaat tagcaaagat attttaattt tgaaagaaat aatccaaaaa atgccagatg
      181 tacaaatgtt ggttcaacct aaaaaaggcg tttcgcttat tggaaaaaaa gaaacacttc
      241 gaaatttatt atccagtcat aaaatcaata tcgcagactc tccggaggac cgtctaactt
      301 atgtgtttgc taaattattg aatactaatg attctctaaa gattcaaaat cttgctgacg
      361 aattatacgt gagcaggtcg acggtagaaa gcacaatgaa agatgttaga aaaagactaa
      421 acaagcatgg gatgtcctta gtttcagatc gaaatggcgt gcgcatcaaa gctgatgaaa
      481 aaagtaaaag aaggttgatg tcagaagttg tgaactatta ttggggtggc tttacagcat
      541 ctaacagtaa aacagatctc ttagacttga atatcaattt cattggtgaa gcgaataatt
      601 tcttaaataa ggacatattg aaaaaagtag ccgaattgct taatcgatac atgaataaga
      661 cacaattgaa atttacagat tatgaatttc aaactttagc tatccacctt gctattgcga
      721 tagaaaggat tgaaacaagc aatttcttat cacgtagtaa taattctgaa gaagaaatct
      781 caaagaatac acttatttta atcaaaatga ttgaaaaagc attttcaatt attgtgccaa
      841 tagatgaaca agcttatgtt gacattcaca ttacttcgat tgaaaaaaat gctatgaatc
      901 acgacttaga tcaaacgaag ctttcctatt tagatatggg tgttgagcta caagagttag
      961 taagaattaa attaaatgaa ttttatcctg atgaagaatt gatcaataat ctagtattac
     1021 atctcaatgc agcagttaaa agactcaaat tgggagtaaa tatctataac ccttatacgg
     1081 acaaaatcaa atattctttt aaaaggtcat ttatgataag tgtagattta ttagaggaaa
     1141 tcgaagagag attctgtata cattttaatg aagatgaaat cgcttacgta acactccata
     1201 ttcaatcact tttggatcgt tacaaacctg acaaaacgaa agtgatatta gtttgtagta
     1261 gcggatatgg aacatcaaaa ttattagagc aaagaataaa taacggtttt gcagatatgg
     1321 ttgagataga agatgtacta agcatcaatg aactacaaga tagaaaagtc actgatgaac
     1381 ttgttataag cacgattccg attgaaaata caaattttcc tgttattgtt gttagtcctt
     1441 tgatgttagg ggcagatgtc agaaaagtaa aacagtatct acagttaaat gaacaaacag
     1501 aagatgcttt tctaaattta ttagat
//
LOCUS       DAHHZJ010000266         1523 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.271, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000266 DAHHZJ010000000
VERSION     DAHHZJ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.271"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            324..941
                     /locus_tag="NBL02_002839"
     CDS             324..941
                     /locus_tag="NBL02_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCD2573462.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            982..1281
                     /locus_tag="NBL02_002840"
     CDS             982..1281
                     /locus_tag="NBL02_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HCD2573463.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1315..1482
                     /locus_tag="NBL02_002841"
     CDS             1315..1482
                     /locus_tag="NBL02_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCD2573464.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
BASE COUNT          540 a          223 c          316 g          444 t
ORIGIN      
        1 agtgttaaaa aaattgtaaa agttaaagaa gtcaaattct gatgtgaaaa ataaaatata
       61 ccatggaaga cataaaagaa aaactggata actagtttat ttaatggcag accgttaagg
      121 cctgccattt ttatgtattt tagcggaata aatataatca tgcgggagtg taaacttact
      181 gtttcaaaaa gtatactttt tgacatgcct tgattttctt tgtttatgta tggcataata
      241 accacataat acaatttgaa acgtttctga tttaatctga ttgttttgaa acaattttag
      301 tgaaaaaagg gtagataaaa acgatgagaa aattttttta tatccgggtc agtagttatg
      361 atcaatcgat cgaacgacaa ctgatttccg cgaaggaatt aggaatccct gaagaatata
      421 tatttgtaga aaaagccagc ggcaaagatt ttaaacgccc tgaatatcaa ttaatgaaac
      481 ggatgtttcg aaaaggagat ttactctatc tacaatcatt agatagacta ggcagaaata
      541 aacaaatgat tttagaagaa tggcgagaat taactaagga aaagcagatt gatatttctg
      601 tgttagacat gcctctgtta gatactgcta gttacaagca actagatggg ttagagacgc
      661 ttatctctga cttaattctg caattactca gctatatggc cgaagacgag cggaaacgaa
      721 ttaatagccg gcaaagagaa ggcgtagaag ctgcgaaaca aaagggtgta aaattcggca
      781 gaaagaaaat tgctttacct cctgaatttc ctgcgatcta ttctgactgg aaagcaggaa
      841 agataactgc agtctatgct atggatcaat tgggcatgaa acgaaatacc ttttatcgac
      901 gggtgaaaga atatgaacat accttgcacc acttttcttg atagatataa cagctattag
      961 acgggaaggg agagtttgaa aatggactta acgaatcaaa gggtcgtttt tactggaacg
     1021 ctgcagcaaa tgacacgaac ccaagccatc ggtttggttc actctttaaa cggccattgt
     1081 caatcaagtg ttacttcaaa aacaaatttc ctggtttgtg gtcagtattc taagtcgttg
     1141 tttgatgatt ctctctatac aaaaaaggaa caaaccgcgc gggacttgat tgcgttaggt
     1201 catgagatta ccattttatc agaagtcgaa ttttatgcgt taattagtca acagttgaaa
     1261 gaatggcaac ggtatttata atcgttagta aaatttacaa ggaggaaaat ttttatgaag
     1321 cgaaaatctt atgatcgttg gggcattgaa ataactagtt ttaatcgaaa tattgctttt
     1381 cacgtggagt gtgactgtgg ggaattagca aaaagtgttt atcaaaaaaa ttacttttac
     1441 tgtcctaaat gccatcgccg atacggggta catggaacat agaaacgaaa tagtgcggta
     1501 gaaaagcgaa tgaatgtagt ata
//
LOCUS       DAHHZJ010000267         1444 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.273, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000267 DAHHZJ010000000
VERSION     DAHHZJ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.273"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            57..389
                     /locus_tag="NBL02_002842"
     CDS             57..389
                     /locus_tag="NBL02_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HCD2573465.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1008..1349
                     /locus_tag="NBL02_002843"
     CDS             1008..1349
                     /locus_tag="NBL02_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HCD2573466.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          524 a          185 c          320 g          415 t
ORIGIN      
        1 aaaaataaca agaaaaactt gttaaaaata tttaaagaaa ttgggtggtt tattttatgt
       61 catatacagg aattttgagt ctcaaagata tttgtcacta cggtaaacgg tgtaccgcta
      121 cagagaaaat cacgaagaaa ctatcaactg gccaaagtaa aaccgttgtg cagtgtaaga
      181 aatatataat ccagaaagac aaagtctcag aggaaatgat ttattacatt gggaaacaaa
      241 agcaaataat tttaaaagat cctatcccct taaaagagct atatccaaca atcaaacatg
      301 tttatgacca aaacggtgta ctgattggtc gaagaaaaaa tggagtcttg cgttgtaccg
      361 caaaagggat ggggcgtttg atcggctgaa ttgatcattc tcataacttg tattttatcg
      421 tttttcgctt gcttgaaaat attgtcgaga cttctaaagg aacttagagt cactagaaaa
      481 ataaggagta gacttgatct atcaacaatt cccttcgggg agtgacgggg taggttttaa
      541 cgattggtaa cgtaaaaaat tggtcaatcg agtaaatgcg cttgtttgga tagtggtgaa
      601 atgaatttgt gtaaataata agcgcagagt aaacaagagg gaatttagaa gttagacaag
      661 ttagaatagc gtctagatta atcatgagca gctactaagg gatctagact ttgacgcaaa
      721 ctcatggaat aaggagtagg tcttctcgta atggggagga cgcacaagaa attgtgtcgt
      781 gtgctgcagt acatgtagat acttgcgagg acgttgatta ttggatcaat cgagcattta
      841 ccgctgtcta ctagggtttc tcgtttcgga cgccataaac gagtcgaaat tttttatgta
      901 tttttaagta aacgaaaaac gataaaatat gaacaagtcc tgaataaagt gaaaaaatta
      961 atataaagca tagagaaaat gaaatttacg taatggagga atgacaaatg aaaaattatg
     1021 atccgaatat acgattgggc acacatacta ttaaggtttc tttccaacgt tgggattaca
     1081 aaggatttgt gacgtttaga aggggcggaa attgtaaagg attagatgtt ttagcattag
     1141 acgaggatga tttatacgat caaacattga ctgataatcc aattggattc ggcttgctac
     1201 ctgaagatga tgaaggtaat gaatggttta aaatgacgct tatgaatgat aatggagacg
     1261 aattatctgt tgaagatact tggtcatatt tgagtgatta tattgtcagc tttgaaatca
     1321 ttgaatttgt agcggataaa gaagaataaa aactggagga ataaatttta cagataaagg
     1381 cttagaagga gtcacgacga ttaataaaaa ggtagtatta ccttaggcat tgagtaagga
     1441 attt
//
LOCUS       DAHHZJ010000268         1373 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.275, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000268 DAHHZJ010000000
VERSION     DAHHZJ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.275"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            150..>1373
                     /locus_tag="NBL02_002844"
     CDS             150..>1373
                     /locus_tag="NBL02_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCD2573467.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYG"
BASE COUNT          443 a          281 c          255 g          394 t
ORIGIN      
        1 cgtacaaaaa aaccgtcttt tcttggctct ataatttcta ggactgatag aaaatcattc
       61 tatcggtccc ttttgagcat gaaaaaacaa aacatacaga agctccgtta gaattgaatc
      121 accacaacac aatcaaagga gcgttctgta tgcctgatct tcatcatatc aaactactac
      181 ttggaatcaa agataaaaac attttcatca caaatgtgga atcgaagagc attaagaata
      241 tcaagagtct tgttgtttct gcgacgttaa gtaaagaaat tcgtcgctgt cctttgtgta
      301 aacagatgaa tcatgaaggc atgatcgtta agaacggaaa gaaaaaatcg ctcattcaac
      361 ttaataaatg tgcgaaccaa ctgacttatc ttgctttagc gaaacaacgc taccactgtc
      421 gtggctgcca cacctatttt acggcgaata cctacattgt cgatagaaac tgctttatcg
      481 ctaaacaagt gcgctataaa attttagaag agctaactga aaaacaagca atgacgacaa
      541 ttgctaagca ttgcggcgtt tcttggtcga ccgtttctcg gacactcgct tctctgatcc
      601 ccatgacaaa agtaaaaaga aattggttac cgcgttgttt attaatggat gaatttcgtt
      661 cactgaaaaa tcaagtcggt ccctactcct tttcttgtat ggatggcgat actggaaaat
      721 tactggatat tcttccttca cggaagaaaa aggctctcgt ttcttatttt atgcaatttg
      781 aacgacgcgc acgattgaag gtaaaggtca tcgtgacgga catgaatgtt tcttatgcct
      841 ctctgataaa ggaatgtttt ccaaaggcaa aactcgttgt ggatcgtttt cacattgtca
      901 aacatctcat tcgtaaattt gaggatattc gcgtacgaat catgaaaagc tttgatcgaa
      961 acgatccaac acaagccaaa tattatcgac aattaaaggc gttgtctcgg cttctgatca
     1021 aacgacagga caagcttgtt tatgacaaat ggaccaaatg gcgcaatttt ggttgggcgt
     1081 atctcaccga aagtgaagtg gtggatcgtc tgctttcgat ctctgacgaa ttacgaatcg
     1141 cctattttta ctatcaagaa attcttcaag cgtttcacga caaagaggcg gacactttct
     1201 ttaaacttgt aagaacgatg cccaattccg ttccaaaaga actgcattct ataaaaaaag
     1261 cttttataaa ctatgaatcc ggcattcgtt tagccctcga attgccttat tcaaatgcca
     1321 aaatagaaaa tctgcatacc cacatcaaag cattgaaacg agtcgcttat ggc
//
LOCUS       DAHHZJ010000269         1364 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.276, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000269 DAHHZJ010000000
VERSION     DAHHZJ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.276"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            80..>1364
                     /locus_tag="NBL02_002845"
     CDS             80..>1364
                     /locus_tag="NBL02_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HCD2573468.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLI"
BASE COUNT          465 a          263 c          257 g          379 t
ORIGIN      
        1 tttaggtatt aaaaaacata tcgttctcta gtatgattaa atcaccacaa aataaacaac
       61 tgagaggacg atacgctata tgaatgattc tatcaaaaaa atgctgagaa taacagagaa
      121 agatttgatg attacagagg tctcttacga gacccttcag aagaaaaaga cgttggtcgt
      181 cgatgctgtt ctctcgccta ctcctcgtgc ttgtagaagt tgtggttcta ctgtggtaga
      241 tggaaacggg aaagcaatta tagtgaaaaa tggaaaaaag gaaacgattg tccgttttga
      301 acaatacaat catatgcctt tggttatgcg cctaaaaaag caacgctata cctgtaaaaa
      361 ctgtcgcacc cattggacgg ctcaaagtta ttttgtccaa cccagacatt caatcgcaaa
      421 tcatgttaga tataaaattg cttctttact gactgaaaaa gtatctttat cttttattgc
      481 gaaaagctgt caggtatctt tgaccaccgt tattcgtaca ttgaaagagt ttaaaagcta
      541 tttaccaaag caatctaaga agatcctccc cagagtattg atggttgacg aatttcgttc
      601 gcatgcttcc atagaagata agatgagctt tatttgcgcg gatggcgaaa caggaaaatt
      661 aatagatgtt ttgcctacgc gtaaattacc tcgattaaca agctatttct taggttgtac
      721 caatccagaa gaagtagaat tcttggtgac agacatgaac gccgcctact tccagctcac
      781 caaacgtgtt ctgccaaatg cgaaagtcgt gattgatcgg tttcatattg tcaaacacat
      841 gaatcaagcg ttcaatgagt tgcgtatccg tgaaatgaat gaacttcgta aagccggaca
      901 gaaaagccag gcagaaaaac tgaaaaagaa ctggcgcttt ttgctaaaaa atcgtgcaaa
      961 catcaaccat tatgaataca aaacatggaa aagtttccga gcaccaaaat acccatttct
     1021 tactgaagca atgatgattg atcgattgct tgagttttct ccgcccctaa aggaggcgta
     1081 tccctttttt catgaattag ttgaagcctt tcgagacaaa gaccctgact tatttttctc
     1141 cttattggca gaacttcccg aaacgttaga tgacagcttt cgggaaaagc ttcaaaacct
     1201 tctgacctat gaagaaggca tcaccaacgc aatgatctat ccttattcca atggaaaaat
     1261 agaagcgaag aatacccaca taaagacaat gaaacgagta tcctacggat ttaaatcatt
     1321 tgagaacatg agaattagaa tctttttgat caatcaatta atca
//
LOCUS       DAHHZJ010000270         1359 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.277, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000270 DAHHZJ010000000
VERSION     DAHHZJ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.277"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..875
                     /gene="ant(6)"
                     /locus_tag="NBL02_002846"
     CDS             <1..875
                     /gene="ant(6)"
                     /locus_tag="NBL02_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HCD2573469.1"
                     /translation="SLAEQDERIRIVTLEGSRANINIPKDEFQDYDITYFVSDIEPFI
                     SNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLTLLPLEELDNYLK
                     GDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNVTPYVIKGLCRKE
                     ILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYISEDLWEKLLSTYR
                     MDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRDMYKKYTGKTGCL
                     DSTYAADIEERREQ"
     gene            872..1120
                     /locus_tag="NBL02_002847"
     CDS             872..1120
                     /locus_tag="NBL02_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin acetyltransferase"
                     /protein_id="HCD2573470.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLLTRGAS"
     gene            complement(1123..>1359)
                     /locus_tag="NBL02_002848"
     CDS             complement(1123..>1359)
                     /locus_tag="NBL02_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HCD2573471.1"
                     /translation="PRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVK
                     GLESRFESILLAYSVLLSRAQRRFGGTLRYSLGY"
BASE COUNT          458 a          205 c          288 g          408 t
ORIGIN      
        1 tttctttagc agaacaggat gaacgtattc gaattgtgac ccttgagggg tcacgcgcaa
       61 atattaatat acctaaagat gaatttcagg attatgatat tacatatttt gtaagtgata
      121 tagaaccgtt tatatctaat gatgactggc ttaatcaatt tgggaatata ataatgatgc
      181 aaaagccgga ggatatggaa ttattcccac ctgaagaaaa gggattttcc tatcttatgc
      241 tatttgatga ttacaataaa attgatctta ccttattgcc cttggaagag ttagataatt
      301 acctaaaggg cgataaatta ataaaggttc taattgataa agattgtaga attaaaaggg
      361 acatagttcc gactgatata gattatcatg taagaaagcc aagcgcaagg gagtatgatg
      421 attgctgcaa tgaattttgg aatgtaacac cttatgttat taaaggattg tgccgtaagg
      481 aaattttatt tgctattgat cattttaatc agattgttcg ccatgagctg ctgagaatga
      541 tatcatggaa ggtcggcatc gaaacaggct ttaaattaag tgtaggcaag aactataagt
      601 ttattgaaag gtatatatcc gaggatttgt gggagaaact tttgtccacc taccggatgg
      661 attcctatga aaacatatgg gaagcattat ttctatgcca tcaattgttc agggcggtat
      721 ccggtgaggt ggcggaaagg cttcattatg cctatccgga gtatgatagg aatataacaa
      781 aatataccag ggacatgtat aaaaaataca ctggtaaaac cggctgcctg gatagcacat
      841 atgccgctga tatagaagag aggcgggaac agtgattaca gaaatgaaag cagggcacct
      901 gaaagatatc gataaaccca gcgaaccatt tgaggtgata ggtaagatta taccgaggta
      961 tgaaaacgag aattggacct ttacagaatt actctatgaa gcgccatatt taaaaagcta
     1021 ccaagacgaa gaggatgaag aggatgagga ggcagattgc cttgaatata ttgacaatac
     1081 tgataagata atatatcttt taacccgtgg tgctagttaa ttttaatatc ctaaagaata
     1141 tctcaaagtt cctccaaaac gtcgttgtgc tcgacttaat agaacactgt aagccagtaa
     1201 tatgctttca aatctactct ctagcccttt aacagaacgt gagttgaaat tttgacaacc
     1261 taatttttct aaggaagaaa aaacagtctc aactgttttt ctttgttttc caagtaagct
     1321 gtggtctagt ttttctgatt tatccatgtt tttacgtgg
//
LOCUS       DAHHZJ010000271         1350 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.279, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000271 DAHHZJ010000000
VERSION     DAHHZJ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.279"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(<1..325)
                     /locus_tag="NBL02_002849"
     CDS             complement(<1..325)
                     /locus_tag="NBL02_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HCD2573472.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLI"
     gene            complement(326..1234)
                     /locus_tag="NBL02_002850"
     CDS             complement(326..1234)
                     /locus_tag="NBL02_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HCD2573473.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
BASE COUNT          445 a          237 c          141 g          527 t
ORIGIN      
        1 taattaaatc aaatatatta tcttctcttt ctagttgtaa gaatgaatca cgtatcactt
       61 caattttccc tacagtatct gtagctaatt caggtttacg ttctacaatt ttcacagacg
      121 gatttaactt agtcgtcaat tctactaact cattactgtc cgtattcaaa caagtcgtgc
      181 aattatgttc aggatgtttt tgttgataga tatctatcat tgctaaagcg aaatggacta
      241 ggggctttcc tgccaaatat ttcaaatttt tatttttaaa ccccttagac ccagctctac
      301 cacaaatagt aaataaaatt tccatttatt tctcctcagc tattttcaat acttcgtttg
      361 catgatataa atcattccag ttttctttgc ctgttactac gttttcaaag aaatatttca
      421 actccttctc atacatatca ttagcagatt caggcatttc tttaacaatg ttttcttcaa
      481 aacttatttt attatttaat aagtcaacat tgatacattc atctaaagta aacaattgaa
      541 attttcttgt aggaactctt ccaaaataat ctaaatgaat ttctataatt ttatcatcgt
      601 atctcaatat atatgttgct aaatcattac tgttaatttc aagattcgaa tatttaccaa
      661 actgtttttt tactgattta gggaaaccaa ataggtttat aatataatcc aattcatgta
      721 tacagtctaa ttctactcct ccccccatag atttaaaggc agaataatta tttctataat
      781 cagtaccttt tctccaatca ggaagataag tagaacaaat agctcttaca gaatatactt
      841 ttttactttg aatactatta cgaacaactt ccatgatatt tttatacctc agtggagcag
      901 caacataaat atttttgtaa tcataattat attgagtaag atcaatactc tcagaaaaag
      961 caggtttttc taaaaatatg taatcaactt tttcaacaat tacatttagt gtttccagat
     1021 gcttaaaagt cggatttgta ataaaagcta tatcataatg tgtatccaaa tcgtctctat
     1081 tttgaatata cgttatttct tcaaactctt tttttccact gctactataa gctgttatat
     1141 caaaattaat agaattcttt tttccatagc ttattagatt ttttagatgt ctctttccaa
     1201 ttgaccctaa cccaaaaaaa attactttca tcattaattt acctcaatgt cgttaattaa
     1261 gttaataata tacttatctt tttcaaaagt atattggggt tccattttct tttccaaaag
     1321 cgtgaaaaat tctttaatct cctctaaata
//
LOCUS       DAHHZJ010000272         1345 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.280, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000272 DAHHZJ010000000
VERSION     DAHHZJ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.280"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            134..346
                     /locus_tag="NBL02_002851"
     CDS             134..346
                     /locus_tag="NBL02_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573474.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            508..1026
                     /locus_tag="NBL02_002852"
     CDS             508..1026
                     /locus_tag="NBL02_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCD2573475.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
     gene            1033..>1345
                     /locus_tag="NBL02_002853"
     CDS             1033..>1345
                     /locus_tag="NBL02_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573476.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SI"
BASE COUNT          526 a          161 c          212 g          446 t
ORIGIN      
        1 taaaaacact atatattccg aaaagtgagt atctccattc ataaaataat caacatattt
       61 aaaaaatcta attagctttt ttagtcaatc taaagttctt tattctatga tataatgtta
      121 ctgtagaaag tttgtggcgg tttggcaaat ccaattgaag gagcgtggtg cctatgggct
      181 tattagctta tcggacgtac tctgtagaaa ggaaatgcct gatttgttct cattggatca
      241 aatcattgct attatcggag cagcagcagt agttattact gccgtagcga aagctagcga
      301 ttcgatttta gcttggttaa agttcaaaaa agaacaaaaa aaatagagcc gtctcaactt
      361 ttggcgaggt agacgagctc taaagcttta tttacatgcc atccgctttg aacggactac
      421 actgggcatg tgacagcatg ccctttcatt tatattatat ctcattcttt tatataatga
      481 aagtaaataa atcatggagg ataaaatatg gataaattta atagattaac attaataaat
      541 cagtttgaaa ttttaaaagc tttaaatcct aacgaagaaa aatactatgc tgaaaaaata
      601 gaaatactaa cagaaggtta cgaatatcac tatagtgaaa tatttgaaaa tatttctgat
      661 ccattgccag aagaagattc gcggtttgtt ctagacatat taaatatgta tagagatatt
      721 acttttagta aaaataaatt aaaagatata aattcaattg ataattatta tatacaattt
      781 aaaggttttg actttaatag ctctaccgaa tttaagttag cactttatgc acaatttttt
      841 attgaaaaat taaataggtt tcaagaaata gttgaagata gtgattttaa cacttttaat
      901 agccataaac cgatgagagg tacttatata aaatttctcg aaaattataa tgatttaaaa
      961 tctaccaata attatcaatt tggaaacttg tcttatgaga tgataagtaa agttctaagt
     1021 ttgtgatgat aaatgaaatt aaatagtata aatgatttat ttgctattat aggtgttatt
     1081 gctaacatac ttttttcgtg tttgaatttt agatttactc aaaagaacaa aaagaacaaa
     1141 ctcagtatag aacaaatgaa aaaagacatt gaaactgatt taaaaaatga ccataaagaa
     1201 agaagaaaaa ttgaagaata tacttcttta atggaaaagt gtaaagcaac tgatgcaatt
     1261 tttagctttt taagaaaaag tgatgagata atcacacgtg aaaatataga tttagaaaat
     1321 gagattagta aacttgaatc tatct
//
LOCUS       DAHHZJ010000273         1339 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.281, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000273 DAHHZJ010000000
VERSION     DAHHZJ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.281"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(<1..615)
                     /locus_tag="NBL02_002854"
     CDS             complement(<1..615)
                     /locus_tag="NBL02_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2573477.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLK"
     gene            complement(626..1294)
                     /locus_tag="NBL02_002855"
     CDS             complement(626..1294)
                     /locus_tag="NBL02_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCD2573478.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
BASE COUNT          494 a          224 c          175 g          446 t
ORIGIN      
        1 tttcaaatag taaaaacgat gttcagctat aacaatagta aatcctttat tttttaaatc
       61 acaaagtaag tcagcaagtt tcttagtcgc tttgaagtcc aaatttgaag acggttcatc
      121 caatataatt aatttagagt ttagcgataa actacatcca atagaaattt tctgccgttc
      181 accacttgat agcgaaaaaa tgtttttatc taatatattt gaaagatcta ataattcagc
      241 taattcattg attcttttat ccatatgctc ttttgtaaat ccaaagtttt ccatcgaaaa
      301 tactaactca ctcgttgtat ttgttgtaaa gaattgtcct ctaggatctt gacttacgta
      361 gccaatattt tttgatatac tttcttgtgt taaggattgt agcgagttat tttcaaataa
      421 aatttcccca accaattttc cgtcataaag tcttggaata agtccattca ttaactttaa
      481 tagtgtggat ttcccactcc cagaagaacc agtaatcaat gtaagactgc ctttttttat
      541 tgaaaaatta aggtcaacaa gcgcttgttc cttaacatcc ttatatgaaa aagaaacatt
      601 gttaaattca aacaattctt tcacctcaaa tcaaaatact taagaaaaaa atagtaacgg
      661 aaaacaggat gatatagtct aataagctca tgtttgtttt atagtaagaa cttcttttca
      721 caggagattc tatccccttt gttattgcat tagcggtcat ttcttcagca atcatagttg
      781 cacgtataag aacgggaact aacagaaatt ccaaatattg cagaggctga aaaaaagaca
      841 agcgaatgtt tctcattttt aaagattctt taatcattct tatttcagaa cgataagtcg
      901 gaaagaatct gatcactact gtaatactta acattatttt tttcggtaaa tgtatttttt
      961 ctaaagagga taataattca ttgctagtat agacactaac caacaaccat gagatcatta
     1021 cgaccggcaa cattcgaaat acaatatata aggatgtcgc aaaaaatata gtaaaaaact
     1081 ctatcttgat agcatcaata attttcatta ctaaaaacag aactgaataa atcaagatga
     1141 aataaataca tgtctttatt ttacgaaaca tcaatactaa aactgtagca ataatgaaac
     1201 accccacttg aaatatattt ccctttccta aaaaaataat gaccggaaaa aaaatattta
     1261 aagtaatcaa ggttaacgga ttgatttttg acactctttc acctccaaaa aataagatcg
     1321 cactgttatc ttaagctga
//
LOCUS       DAHHZJ010000274         1333 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.282, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000274 DAHHZJ010000000
VERSION     DAHHZJ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.282"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(242..856)
                     /locus_tag="NBL02_002856"
     CDS             complement(242..856)
                     /locus_tag="NBL02_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCD2573479.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          442 a          258 c          221 g          412 t
ORIGIN      
        1 tttttatttt tctgacacta catattctta taactcattt ctcataactg tgaaacaact
       61 tataaaaata acaaacttta aacaaaacaa gcttaaaatc aaagatgatc catcttaaaa
      121 taactaaaac aaaaaaagta gtcgttgtta atcctcatta ataatcgttt tctttttttc
      181 aaacgctcca tttttatgtg gaacattatt tttttaagga actttgtcaa catgtggata
      241 actattttcc acttgtgtcc tggactagct gttttgctgt aaaatacaaa acttgctgca
      301 gcaaaaagat cgttgcaaga gaggctgttc cgcagatcgc tggatctaga tttggcaaag
      361 tcaagactgc atcatgtaaa ttggcgtggt tgtccaaaac caaatcagca aattcaaata
      421 atcgcaaacc actgctatgt tgcgatttga tacttcgtga caaatcaaat ccagtaacaa
      481 tgattaatgg atgaccattt tctttgatcc actgagctag ttcgatgatt gccgggtttc
      541 ttccttcatt cgaaagaaga agaaaaatgt cttctgattt gacagaaagc tgatcaataa
      601 ttgcctgtcc tgcaccttct agcgtttcat agacacctcc ggctggattt tcaatcaata
      661 tacttggtac aagatccggg gtttgatccc aaaattcaaa tgctacacca cttagtgtgc
      721 ggctagcata tatctgcaat ctaccacctt gattaatttg tttcgcgatc caagtgccag
      781 cttgaagcat cagcggctct tctatcctta atttctcttc caattggtga taatagtcaa
      841 aataacataa actcatttat ctctcccgcc tagctatact tgacatgaat agaaacagct
      901 gcctaatcta aggaagtaca taaaaaaaca gtagttagac agtaacacat gtggtctttg
      961 ctgtttcaaa cacaaaaaag gcattgaacg aatacgccca ataccttgac atagcaaata
     1021 aaacaacgtg cagaccatgg aaaatatcta cataggggta gaattttcac cctagattag
     1081 gtacaataaa ttaggtcgat gcactgcatt gactatggca aaatgatcgg tcagggtgta
     1141 tgtgtgtgac tggtcattcc ctgattctta gcgccaacta agggtcaggg agcctttttt
     1201 tattcagctg tttctatctg aatttattgt aagtaatctc ttatccaagt gcaagcaaga
     1261 aaatacaaaa aaacattggt atgatttata tatatataaa taacataaaa aaaggaatgg
     1321 cttatcccga tac
//
LOCUS       DAHHZJ010000275         1319 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.284, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000275 DAHHZJ010000000
VERSION     DAHHZJ010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.284"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..1244
                     /locus_tag="NBL02_002857"
     CDS             <1..1244
                     /locus_tag="NBL02_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCD2573480.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          427 a          238 c          285 g          369 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgtacaggta ctgttctatg gggcgctta
//
LOCUS       DAHHZJ010000276         1313 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.285, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000276 DAHHZJ010000000
VERSION     DAHHZJ010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.285"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..274
                     /locus_tag="NBL02_002858"
     CDS             <1..274
                     /locus_tag="NBL02_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HCD2573481.1"
                     /translation="VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQC
                     GIIKMRDYLILDIDSEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            279..1226
                     /locus_tag="NBL02_002859"
     CDS             279..1226
                     /locus_tag="NBL02_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCD2573482.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          435 a          189 c          270 g          419 t
ORIGIN      
        1 agttactata gttaataaat caagatacac atctaggcaa cagacacctg aagtttttga
       61 aatgaatgcc tcaatgtatt cttttaaacc tgaatttcta aaaaaaagta attatatttt
      121 tgatgggcaa tgtgggataa taaaaatgag agattatcta attttagata ttgatagtga
      181 agaagatttt aaatggttag aatttttgtt tcctaaattt ttagaagatt cttcggagct
      241 tcaagatatc tataattatt tatatcggag gtaaaaggat gagctgttat cttatcaccg
      301 gtggggctgg cttcattggt tctaccttag ccaatcattt aggagaagaa catgtggtaa
      361 tcgtcgtcga tgacttatca atgggaaaag tagaaaatct tgatatgacg aaaaatatca
      421 cttttattga gggagatgtc gctgagaaat ccttgatgga agagatcatg aaagctaata
      481 aatttgatta tatctttcat ttagcagcag ttgctagtgt ggcagattca gttgcccgac
      541 cagtagaaac acatcgtgtg aattttgaaa gtgtattaat gattttagaa ttgatccgca
      601 agtatcaacc tgatttaaaa cggatcgtgt ttagttcttc agcggctgtt tatggggatg
      661 aaccgacttt accaaaaaaa gaagagtccg tgattcgccc attgacgcct tatgcggttg
      721 ataaattcgc tgcagaacaa tatgtgctag attattgtca tttatatgat gtacccggta
      781 gcgcagttcg ttttttcaat gtctatggtc caaatcaaaa tccgaattct ccatacagtg
      841 gtgtgatttc tattctcgta gatcgctata agaaacaaat cgtaggagaa gcaacttcat
      901 ttacattatt tggtgatgga agccagtcta gagactttgt ttatatagat gatgtaatcc
      961 aagcattact tttagttgcg aatgaggaag ctgcactggg gaaacaattc aatgttggaa
     1021 cgggtaaagc aaccacactt ttggaattga ttcatgcaat caatgaaatt ctcggtgtag
     1081 aattgacact tgaatacaaa gaagagcgtt cgggtgatat ccatgactct ctagcagata
     1141 ttactaagat ccagtcaatt ggctatcaac cgaaatatgg tattttaagt ggaatgacta
     1201 attatttgaa aactgaaatc aattaaaaca tactaagata agattagagg gtaaattcag
     1261 gctctttgtc aataaggact gatgagtttt gtaaaatcaa atctgggata gaa
//
LOCUS       DAHHZJ010000277         1297 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.286, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000277 DAHHZJ010000000
VERSION     DAHHZJ010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.286"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            7..624
                     /locus_tag="NBL02_002860"
     CDS             7..624
                     /locus_tag="NBL02_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573483.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
BASE COUNT          487 a          167 c          205 g          438 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaaattat
      841 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt
      901 taggtctatt taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc
      961 tttcgtactt aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac
     1021 tagtatgata tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt
     1081 cctaaaacct ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa
     1141 catgaaatta tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa
     1201 catttattga ttgcaaaatt atgtgacaaa aaaactagta gaagaaagaa taatggcttg
     1261 ataataaagt actacctatt aaaggtagta ctttatt
//
LOCUS       DAHHZJ010000278         1289 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.287, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000278 DAHHZJ010000000
VERSION     DAHHZJ010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.287"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..692
                     /locus_tag="NBL02_002861"
     CDS             <1..692
                     /locus_tag="NBL02_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCD2573484.1"
                     /translation="DESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFTAI
                     LVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVGKF
                     IRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGYAQ
                     VYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVISLA
                     EIAFPNEKIFR"
     gene            708..>1289
                     /locus_tag="NBL02_002862"
     CDS             708..>1289
                     /locus_tag="NBL02_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCD2573485.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIF"
BASE COUNT          448 a          217 c          242 g          382 t
ORIGIN      
        1 aagatgaaag tattattgaa gcatctggat ttagaatcca acctgaatat gaactattca
       61 aacgtttgtt tgatatcatc gtttcacttg ttttattagt tattgcttca ccatttatgt
      121 tattcacagc aattttagtc aaagtgacat caccgggacc aatcatctat aaacaagtgc
      181 ggatcacaaa aaatcaaaag gaattttcaa tctataaatt ccgctcgatg accgccacag
      241 ccgaagcaaa atctggacca gttttagcta agagcaatga tgcacgagta acgcctgtag
      301 gtaaatttat tcgagctgtc cgctttgatg aattaccaca aatcttcaat gtgctgaaag
      361 gcgatatgtc gatcgtagga ccacgtcctg aacgtccttt cttcgtcgat cagttcaatg
      421 cagaaaaccc ttattactac ttacggcata atgttcgtgc tggtatcact gggtatgcac
      481 aagtctatgg aaaatatgtc tctgattaca acagtaaatt acgttttgat ttactttata
      541 tcaaaaaata ttcattgatg cttgatttaa agatcttgct gcaaaccatc aagatcctat
      601 ttgataaaat gtcctctcaa ggacttgaag aagacgaaaa tgtgatctca ttggcagaaa
      661 ttgcttttcc gaatgaaaaa atctttcgct agttgggagg cgtaaaaatg aacaatccga
      721 aattcagcat aatcgtacca gtatacaatg cagaagcaac tattggtcga acgattgata
      781 ttctaaaaga attggattat gaagagtacg aagtcgtttt agtaaatgat ggatcaacag
      841 atcaaacaca ggaaatcatt caagaactga ttgaaaatga tgaacgtttt gaattgatta
      901 ctacgaccaa tcaagggccc ggattagcaa gaaacgaagg aataagacag tcaaaaggag
      961 aatatctctt attcttcgat gcagatgaca cgccagtaaa aacgatcttg tcagattatg
     1021 atcatttgct agaaaagagt ccaaaagcgg atcttgttgt tagttcgttt gtttttcgta
     1081 caatggacga gggaagaatc gtttccgaaa aagaaaactt agttacagaa tgtcactatc
     1141 taactcacga agagtttata aaagacatgt atcagttaat gaatgaacag ttgatgtatg
     1201 ttgtatggaa caaatgctat cgtagcgata ttctgaaaaa taaccaaatt ctatttaaaa
     1261 cttataatag ttgtgaggat cgaatattt
//
LOCUS       DAHHZJ010000279         1287 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.288, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000279 DAHHZJ010000000
VERSION     DAHHZJ010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.288"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(<1..492)
                     /locus_tag="NBL02_002863"
     CDS             complement(<1..492)
                     /locus_tag="NBL02_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCD2573486.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDP"
     gene            complement(494..1213)
                     /locus_tag="NBL02_002864"
     CDS             complement(494..1213)
                     /locus_tag="NBL02_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCD2573487.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
BASE COUNT          467 a          245 c          139 g          436 t
ORIGIN      
        1 tggatcaaaa gaagtgatta ataattcttt aaaaaataga actactataa ccaacactaa
       61 aaccgaaacg ccaattgtaa tccacttatc aatatcttgt acagctaaaa tatttccaaa
      121 taaaatatga aataaatccg ttgagctatt tgcaactcct ataaggataa ctcctagtgc
      181 taaaaaagaa ctgaaagtaa taccaattgc tgtatctcct tttattacac tattgctttt
      241 tataaaagta ataatgatag aagctaatag accaaataca atcgcaccaa taaaaaaatt
      301 aatacctaaa atataagaaa ttgctactcc tggcaaaaca gcatgagaaa tagcatcacc
      361 cataagagac attcctctta aaataataaa acaacctact gttccagaaa caatccctat
      421 aactacagat gtaattagag cattctgtaa aaagtgaaag tcatacagac cattaataaa
      481 attttgaagc atattattcc ccctctccaa taaatatagt atctccatag gcttttttta
      541 aattttcttc attaaataca gattctacac ttccgtgagc aattagattt cgattcaaaa
      601 gaataatatt atcaaaatac ttctttacct tgcttaaatc atgatgcaca attagcactg
      661 tttttccctg atgtttcaat ttttttaacg tatccataat tattcgttca ctaactaagt
      721 ctatccctac aaatggttca tctaaaaata tgaaatccgc attttgagca agacatcttg
      781 ctaaaagtac acgttgaaat tgtcctccag ataattctcc tatttgatga ttagaatatt
      841 ttcccatatc gaccttttcc aaagcctttg aaactctttg ccattctgca tttttaattc
      901 tttgaaaaac tttcattccg gcatatgttc ccatagatac acattctttt acttttattg
      961 gaaaagtaaa atcaatatca gctttttgct ctacatatgc aatcttcacc agttttttat
     1021 tgatatcttc attattaatg gagacctctc cctgatgagg gataatattt aacattgctt
     1081 taagcaatgt ggattttcca gcaccatttg gaccaataat acctgttaaa gcaccttctt
     1141 gtatttttaa agaaatctcg ttcaatgcta aaaaatcgtt ataacaaaca ttaagctttt
     1201 tcacttctaa cataactttc tccttctaaa tgataatgat tatcacgatc atatggtata
     1261 caaaaattat attttagtca accctaa
//
LOCUS       DAHHZJ010000280         1282 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.289, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000280 DAHHZJ010000000
VERSION     DAHHZJ010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.289"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..>1282
                     /locus_tag="NBL02_002865"
     CDS             <1..>1282
                     /locus_tag="NBL02_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HCD2573488.1"
                     /translation="NDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHTC
                     RNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQS
                     YFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSKR
                     ILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEEV
                     KFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKSQ
                     AEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAYP
                     YFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNGK
                     IEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLI"
BASE COUNT          429 a          245 c          246 g          362 t
ORIGIN      
        1 aatgattcta tcaaaaaaat gctgcgatta atagagaaag atttgatgat tacagaggtc
       61 tcttacgaga cctttcagaa gaaaaagacg ttgatcgtcg atgctgtttt ctcacctgct
      121 ccccatacct gtagaaattg tggttctact gtggtagatg gaaacgggaa agtaattgta
      181 gtgaaaaatg ggaagaagga aacgattgtc cgctttgaac aatacaatca catgcctttg
      241 gttatgcgct taaaaaagca gcgctatacc tgtaaaaact gtcgaaccca ttggacggct
      301 caaagttatt ttgttcaacc cagacattca atcgcgaatc atgtcagata taaaattgct
      361 tctttactga ctgaaaaagt atctttatct tttattgcga aaaattgtca ggtatctttg
      421 accaccgtta ttcgtacgtt gaaagagttt aaaagctact taccaaagca atctaagagg
      481 attctcccca gagtattgat ggttgatgaa tttcgttcgc acgcttccat agaagataaa
      541 atgagcttta tttgcgcaga tggcgaaaca ggacaattaa ttgatgtctt gcctacgcgt
      601 aaattgcctc gattaacaag ctatttctta ggctgcgcta atcctgaaga agtaaaattt
      661 ttagtcacag acatgaacgc cgcctacttc cagcttacca aacgtattct gccaaatgcg
      721 aaagtagtga ttgatcggtt tcatattatc aaacacatga atcaagcctt taatgagttg
      781 cgtatccgtg aaatgaatga acttcgtaaa gtaggacaga aaagtcaggc agaaaaactg
      841 aaaaagaact ggcgcttttt gctaaaaaat cgtgcaaaca tcaaccatta tgaatacaaa
      901 acatggaaaa gttttcgagc accaaaatac ccatttctta cggaagcgat gatgattgat
      961 cgattgcttg gcttttctac gtccctgaag gaggcgtatc cctattttca tgaattagtt
     1021 gaagcctttc gagacaaaga ccctgactta tttttctcct tattggcaga acttcccgaa
     1081 acgttagatg acggctttcg ggaaaagctc caaaaccttc tgacctatga agaaggcatc
     1141 accaacgcga tgatctatcc ttacaccaat ggaaaaatag aagcgaagaa tacccatata
     1201 aagacaatga aacgagtatc ctacggattt aaatcatttg agaacatgag aattagaatc
     1261 tttttgatca atcaattaat ca
//
LOCUS       DAHHZJ010000281         1271 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.290, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000281 DAHHZJ010000000
VERSION     DAHHZJ010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.290"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(12..>1271)
                     /locus_tag="NBL02_002866"
     CDS             complement(12..>1271)
                     /locus_tag="NBL02_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573489.1"
                     /translation="FSIIYTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRD
                     YQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTF
                     FLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLFLFLGLI
                     GAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNGFSSLFI
                     AYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLTLIAPEDAVAI
                     AENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIELDNSNNKNTVL
                     TTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTKLAIASRYLSI
                     VTLILFLLYRFTFSKQKQPRREVQHSH"
BASE COUNT          483 a          183 c          208 g          397 t
ORIGIN      
        1 taaataaaaa tctagtgaga atgttgtact tctcttctcg gttgtttttg tttagaaaaa
       61 gtaaatcgat aaagtaaaaa taaaatcaaa gtaacgatag ataaatatct tgaagcaatt
      121 gctagttttg tgtactgata agtaataacg actttattta tacctggagg aatagttaat
      181 tcagttgtgc caaacttaga taattttgtt tgtactagtt tgccattaat agaagccact
      241 tgccctttat aacgataaat aggtgtcgtc aagacagtat ttttattatt cgaattatct
      301 aattcaattg taagtttgtt actcataaaa tgagttgttg gatttatttc ctggttacct
      361 agtaaatatt gatcattatt aatcatatca ggatgataga ttgactcttt gtttgcataa
      421 tcagtatgat tgtaattttc agctatagca acagcgtctt caggtgctat aagtgttaat
      481 gaatctttgt tagcatggat agaattgctt actcctgaga gatgcaatat tagtgcaaga
      541 aacgtaaaga ccattatttt ccatgaatta tttttagtag aaaaaatata atagatcact
      601 atactcatcg tataggctat aaataaagaa gaaaaaccat ttaaccgcca gacaaattgt
      661 agactcttaa ctggtgtatg gttaaaaagt tgccaaggaa ataaattggt tgaacaaaaa
      721 agaacaaata caccaagaaa gaaaataaat aaatcatcag cagttaattt ttttataaaa
      781 ataaaagcac caatcaatcc gagaaataag aataatccaa tagtatagtt gttatagtta
      841 ttatttaaaa tatgtgtcag catatcacta ggagccatac cacttaattc ttttcccagt
      901 gggactttca attcttgtgc tctagattgt tgaataaaag gaattaagaa tcccgcactt
      961 aaaaaaaagg tgaggacggt agcttttaaa agacttaaga gtctagcgat tttttggtcc
     1021 caaaaataaa aagaaagaaa caacgttcct ccaataaata cagaaaccat agcgacagaa
     1081 agtaaatgag tatacactac tagcgtcatg ccaatagaca accagtacca tttttggtaa
     1141 tctctaatat aaatttcgta acaccccatt agtatcaaag gcaaaaatgt taaagcgaca
     1201 gcttctccca atgaagcacg atggaatatg tcaatggcac gataggcgga aaatgtataa
     1261 ataatcgaaa a
//
LOCUS       DAHHZJ010000282         1241 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.291, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000282 DAHHZJ010000000
VERSION     DAHHZJ010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.291"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(277..354)
                     /locus_tag="NBL02_002867"
                     /pseudo
     CDS             complement(277..354)
                     /locus_tag="NBL02_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(508..1143)
                     /locus_tag="NBL02_002868"
     CDS             complement(508..1143)
                     /locus_tag="NBL02_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD2573490.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
BASE COUNT          358 a          261 c          176 g          446 t
ORIGIN      
        1 atacagatat tctccggata ctttagaatc acatgataaa ggtattctta aactgggtac
       61 aaaaaactaa gccctcctaa aaaaggacat ccaattattt gttcccacta tcaaattgac
      121 agtttattta agaatacctt gccgcatatt tattaactcc ttttaaatag atacttaaat
      181 aatagcacgt aagagcatat ttgtaaagga atctccaatt ttttatcaaa gagagtacgt
      241 gattacaaaa tagctgtaat aatgtaccaa tatttgttat tctataatct tccaattact
      301 cccgttcttt tcaagtacca aatcaaattg agatacctgc gttgcctttg cctgatttac
      361 cacaaatcag attttatccc tttcttcctc ttgattgccc acaacagaaa agaaaaagcc
      421 ctgtcaagag ctttgccacc attggtggtg ctttgcactc ttgatcgggc tttttgtttg
      481 ctgtatcttc tcgcaagagg aaaaaagtta ctcgtcctca tcatcaaaat tgttattcct
      541 taatacttct cgtaacagtt ccatatcttc ttttgagttc gggtttatca ttttcacgac
      601 ttgataaggt gtcttatact tatgtccctc tatgctttcc ccaaacatat ccatactgta
      661 taaatcatca tagcggtcta ataatttttg aaacttcaaa tgctggataa attctttgat
      721 tgggtaaagg tcggacgctt caatgctttt cccgttgata tagtctgtaa tatattcccg
      781 taacttttct aactcatatt ccagccattc ttcctcgctg tcaaagaagt tatcatagtg
      841 tccatgtttc agttctgcat ttagacgttc ttcggctctt aaaaactgac agacttcctt
      901 ttcagattga ttgacatact tccattctcc cgacaacaat tcattgggcg taacgtccag
      961 cacttccatt atcttttcca gcgtttcata ggcaggatag ttcaaccctc tttcaatctt
     1021 ggaaaggctc tgcatattga taccgatttt gtccgcaagt tcctgttgtt tcattcccct
     1081 aaattttctt atggtctgta tattatttcc taaatggctt attttcattg cataaccctc
     1141 catatctcgt tatatcttcg ctattataag tctatcatgc ttaatatgat ttgtaaaggc
     1201 aaaaaaagat attgacaaat aagcatataa gaattactat g
//
LOCUS       DAHHZJ010000283         1221 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.292, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000283 DAHHZJ010000000
VERSION     DAHHZJ010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.292"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(<1..113)
                     /locus_tag="NBL02_002869"
     CDS             complement(<1..113)
                     /locus_tag="NBL02_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCD2573491.1"
                     /translation="MKKKIFVGAIIALFLLPINAFAYSINNEFNLGANEGSS"
     gene            complement(110..529)
                     /locus_tag="NBL02_002870"
     CDS             complement(110..529)
                     /locus_tag="NBL02_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCD2573492.1"
                     /translation="MEKYLNTLSVMTGVIGGTIVGLLGGMDNILHVLIFLVGVDFLTG
                     LAKAWKLKEISSEIGFEGLLKKVLIFVVIAVAVDVQKIVGNSIPLREIVIMFYVANEG
                     ISFLENISVFLPLPDKLKEVFQQIRNDTENKDRGGTK"
     gene            complement(599..739)
                     /locus_tag="NBL02_002871"
     CDS             complement(599..739)
                     /locus_tag="NBL02_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HCD2573493.1"
                     /translation="MNSFPGFENIKQFYDWGCYTDQDLLDYVNMNCLTKDQYKQITGN
                     EI"
     gene            complement(732..1079)
                     /locus_tag="NBL02_002872"
     CDS             complement(732..1079)
                     /locus_tag="NBL02_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573494.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKSGTAVDDIEDVDQLKQLNALLTKQLAISVQEQEKMQQMLAQLTM
                     EVASIKNGGKSNE"
BASE COUNT          352 a          262 c          170 g          437 t
ORIGIN      
        1 gaactacctt cgtttgcgcc caaattgaat tcattgttga tcgagtaagc aaaggcgttt
       61 attggcaata aaaaaagagc tataatagct cctacaaaga ttttcttttt catttggttc
      121 ctcctcggtc tttattttca gtatcatttc gtatttgttg gaatacctct tttaacttat
      181 ctggcaaagg aaggaatacc gaaatgtttt ctaaaaaact aatcccttca tttgcaacat
      241 agaacataat aactatttct cttagaggta tagaattccc aacgattttc tgtacgtcaa
      301 ccgcaacggc aatcacaacg aagattaaga ctttcttcaa caatccttcg aaacctatct
      361 cactacttat ttctttcaat ttccatgcct tagcaagtcc agttaagaag tccacaccca
      421 caagaaaaat taaaacatgt agtatattgt ccatccctcc caataaacca acaattgtcc
      481 cgcctatcac ccctgtcatt actgagagtg tatttaaata tttttccaat atctatttct
      541 cctttatgca aagaagcgca ctcgtttgag tacgcttttt atttaacttt gtgcttaatt
      601 aaatttcatt tccagtaatc tgcttatatt ggtcttttgt taaacagttc atgtttacat
      661 agtcaagtaa atcttggtcc gtataacatc cccaatcata aaattgtttt atattttcaa
      721 agcccggaaa actattcatt acttttccct ccattcttaa tacttgcgac ttccatcgtc
      781 aattgagcta acatttgttg cattttttct tgttcctgta ccgatatcgc caattgtttt
      841 gtcagtagag catttaattg cttcaactga tcgacatctt caatatcgtc aaccgctgtg
      901 ccactcttct cttcttcagt agctaactca acccactgtt ttttatcaaa atcaaatctt
      961 ggtctccagt taggaactgg tggtttaatc tctgtacagt tttcagggat attttcttga
     1021 ttgttcaaaa tgatttgctc gaatccgtaa ggcttgattg atttgtaaac tactttcatt
     1081 agatttcctc ccttaaattg aataagtgat aacaaatgaa taagctgatc cataacttga
     1141 atttcttctc catttaatgg ctccgtctgc gccaatagat agttgagcac tgttcaaagt
     1201 agaacggtct atcgagccaa c
//
LOCUS       DAHHZJ010000284         1173 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.295, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000284 DAHHZJ010000000
VERSION     DAHHZJ010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.295"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(297..782)
                     /locus_tag="NBL02_002873"
     CDS             complement(297..782)
                     /locus_tag="NBL02_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573495.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(921..1136)
                     /locus_tag="NBL02_002874"
     CDS             complement(921..1136)
                     /locus_tag="NBL02_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HCD2573496.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
BASE COUNT          436 a          199 c          187 g          351 t
ORIGIN      
        1 taagaagaaa atatgtcaaa aggtaaaaaa gtaaattctt ctatccaaat atagaaaaat
       61 tgaatctaga aaaccgcccg acaataaatt tcattaactc agttgaccga aaatgaagta
      121 gataccaaaa atttatcaag agagcaagta gagaagtgag tatattcata agagaaatag
      181 ctgtaacgaa ttattaggtt aatgcaaatg cttatttata ctccgtataa ctttctcaga
      241 aaaaatgata ccaataatga cttgctagta ctgtaaatcc gtataatatc atctaatcaa
      301 aatggtgttc catttgcatc tgtcatcgta atcgatcctt cgggtgacac tcgataaaaa
      361 ccaacgcttc cagatcctcc ttgggaacgg atttgtttgc tggaagctgt gaacaaataa
      421 tctcgatcaa ttttttgata aaagctaata acaatatcgt cactttgtaa ttcggaacgg
      481 tgggcaataa gaaatagaag tgctttgtca gagttttcta atggaagaga aggtacagtg
      541 ctttcttgca tttcagatgt tgaagaaatg ctaaacgact gaatacttga ttggctatgt
      601 tgcgaatcta cattaccagt agaagtatta gtaatggaag tagtagaagt acttttagtt
      661 gaaccaacct cagttgttga aatggctgct gaagattctt taacagaatt ctttgtttta
      721 tatccacagg cagaaaaaag tattacagtt cccaacaagc ctgcgtaaat ccctttatgc
      781 atgatagctc ctcctaaaat agattatctt tattcagtat atcacttaaa caaagaaaag
      841 cagatggttt aaaatgaaaa aaccttccca ataaagggaa ggttcatgaa gataataact
      901 tttttaataa ttatagcttt ctaatatctt tcatcaactt ttctagtaat cattgggaaa
      961 aatttcaagc agtagatcgc acaaatacct acgacgatat aaatgatctt cgcaaaaata
     1021 gttgttgaac caccagcaat catagctact aaatcaagtt cgaacaagcc taccaacagc
     1081 cagttcaacc ctcccacaat caataaagtt aatgcaatag catctaaagt tttcattaga
     1141 attctccttt cttaaatgat aaaacaatta tat
//
LOCUS       DAHHZJ010000285         1150 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.296, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000285 DAHHZJ010000000
VERSION     DAHHZJ010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.296"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..641
                     /locus_tag="NBL02_002875"
     CDS             <1..641
                     /locus_tag="NBL02_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCD2573497.1"
                     /translation="YQSIRRQIIYADLEPGKKVSEKKLEETLSIGRTPIREALIQLRQ
                     QGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQKKRILETIISE
                     QEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERFRWLRVKTEGLK
                     WEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPKYFKNI"
BASE COUNT          412 a          179 c          159 g          400 t
ORIGIN      
        1 cttaccaatc tattcgccga caaattatct atgctgactt agaacctgga aaaaaagtat
       61 cagaaaaaaa attagaagaa acactttcta ttggtcgtac accaatacgt gaagcactga
      121 ttcaactccg acaacaaggt cttgttgata caattcctca aagcggaaat tatatctcat
      181 taattgattt agattcggcc aaaaatgctc gatttgtcag agaacatttg gaacgacaaa
      241 ttatgttaga gtgttgtgcc aaaatgaata accaaaaaaa acggatttta gaaactataa
      301 tttcagaaca agaaaaagct gttagtcaaa aaaatgctcg tagttttttt cataatgata
      361 atttattcca taaagcttgt tttgaaattg cagagcgaga agaagtatgg caatggttag
      421 aaaataacaa tacacactta gaacgatttc gatggctacg agtaaaaacg gaaggattaa
      481 agtgggaaaa aattatggaa caacattatc aactatttga ggcattaaca aaacaagatc
      541 cagaagaagc taactttcta acatcgttgc atcttcattt aatgcttaat gagcaaaata
      601 ttgttttagc tactcatcca aaatatttca aaaatatata acttttaact ctttttaaat
      661 taaatggttg ttctgatcag aaaaatatcg cttgttttct tattatgtaa tatatttcat
      721 aaactattct gcataacaac tattgtttgt actttttaca ttggataact aattatgact
      781 gctgataact tttgatatta aactagtatc tccaatacaa ataaaaaata gatatcttgt
      841 tcatcaaaat tagttcgtgg atacttacct tcatttacat cattcagcgg tttcccattt
      901 tcttttattc tattatttca ttttctaata gacttggctt catttcgcaa tacttttttt
      961 caataggcat caaattggtg ttgtttagat atttagttgt agttttgtga catgataagt
     1021 atgctccttt ttctcttttc cactaataca acgatcaaag ggtgcttttt tcaaaaaact
     1081 agaaaaatca aaataagatt cgtattttga ttctatatat cctgtttcaa ctacatctta
     1141 tatttcacaa
//
LOCUS       DAHHZJ010000286         1132 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.297, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000286 DAHHZJ010000000
VERSION     DAHHZJ010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.297"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            28..303
                     /locus_tag="NBL02_002876"
     CDS             28..303
                     /locus_tag="NBL02_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573498.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            730..>1132
                     /locus_tag="NBL02_002877"
     CDS             730..>1132
                     /locus_tag="NBL02_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HCD2573499.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVS"
BASE COUNT          428 a          195 c          171 g          338 t
ORIGIN      
        1 tgaaaaaaat aaagtcaggt gaaaaaaatg aaacttgagg ataaaaaagt cattttgaag
       61 aaaatcaaac gactaccaga ccaaatagat ggctatacga tctgttcttt caaaaaagac
      121 aggagtataa aagcttatac gcaaaacaca gaaaaaggaa taatctattt cctacatgaa
      181 gaaggttacg atacccaaac ctttatgttt actgaaaaaa aagaatttca taaacagatg
      241 aaaaaactga ttgaaaaaga atttccaaga agtcataaac tctatgtgaa tcagcaattt
      301 taacttctaa ctgttcttta ttaattaaaa atgttctgta ataaaataaa actttataac
      361 aactataaca aaaccgaatg caaaacagaa gttctataag ttataatcat agttgttagg
      421 tgattcatct agtaaaaaca ccagatttca aatttcacac aatttaaact agatcaatca
      481 tcaaccttct tcaaattgtt agaacccttt cccgaggagc tgcagcataa gcaaaaaaac
      541 aaacaggaag ccaaatggct aagacatttt ttctattagt ctagttgttt ctcgtcgcaa
      601 gtttaagact ttagaaagtt atttctaatg atgtttgggt tgttttgatc aatgcttatg
      661 cacagctcct tacctattat ttatctcaac agatctaaca aattaatgga ctatttaagg
      721 agttttttta tgcttacgat ctattcttct cgcacacata gttaccatct gatggcaaac
      781 tggctgaaag accatcaaat gccttacaga gaacgaattt tcacacctaa acagccttta
      841 acaaggaaag agatatttta tttattgtca ttaacagaaa acggatttga cgatctatta
      901 gctaaacgct cgattgatta taaaaatttg aaagaccaaa tcgaccaatg tacgacctca
      961 gagcttgtcg atctgatcgt ctcgcatttt actttgttac aacgtcccat cattactgac
     1021 ggaaagaaat tattagtcgg attcaatgaa gatagcatgc gtctttttat ccctgtacaa
     1081 tggcgcagaa gtcaactgaa atctgtctat aacgatttta catgggtaag ca
//
LOCUS       DAHHZJ010000287         1131 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.298, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000287 DAHHZJ010000000
VERSION     DAHHZJ010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.298"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            85..495
                     /gene="spx"
                     /locus_tag="NBL02_002878"
     CDS             85..495
                     /gene="spx"
                     /locus_tag="NBL02_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HCD2573500.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            509..865
                     /locus_tag="NBL02_002879"
     CDS             509..865
                     /locus_tag="NBL02_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HCD2573501.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          396 a          162 c          211 g          362 t
ORIGIN      
        1 tttttttata ttttattagc taataattgg tattttaaaa gaaattactg tagaattgga
       61 ttataattaa aaaggagtga gaaaatgata aaagtatata cttcgcctag ctgtacttcc
      121 tgtagaaaag caagagcatg gttgcaagcg aatcaacttg aatttgaaga gaagaatatt
      181 tttgccgaac cgttaacaga aagtgagatc aaaaagatct tggcagcgac tgaaggagga
      241 gttcaagata ttatttccac tcggtcgaaa atttacgaaa agctgggcat agattttaac
      301 gaattaactt taaagcaaat gatcgtctta ttcaaagaat atccttcatt attgagacga
      361 cctattttga ttgatgaaaa aagaatacta atcggattta atgaagatga gatccggtca
      421 tttgtccctc gtgaaattcg atgtgttgcc agaaatctga tctatttaca aaacaataac
      481 atcttaaatt catagggagg gagccttcgt ggaagtgaaa gtctatggat cagcgcactg
      541 tgtaacaagt ctgaaggcga aacaatggtt agaacaccat caattgcctt atcaatttgt
      601 tgacttagaa gaacgcgatc taagtacaga agaagcagaa gaattatgca gttttgaaga
      661 agtgcaagca gaacagctat ttgcgacttg gtctgaggct tttcacaaca ttgcgattga
      721 cctttcctgt gtaggaaaaa aacaactgat ttttttatgc acacaacata ccaatctcct
      781 cagacgtcct ttgattatta tcgatgaagt tctatttatt ggatacaatg agcagttgat
      841 ggaacagctt atcttaaata aatagacttt aactgtttta atcaagtcac tgagcatttc
      901 atacagttgt aacagtcgaa cacttagcat caagcgtgtt atgtgttctt tttttgactt
      961 tatttttcta ctatagagac atgctcaatt tatgtggtca aaattcaaat ctaggtgggg
     1021 atatagagtt aaaaatatat tgacatgtgt aaatttacat gatatattca tttctgttgt
     1081 tttaagtagc aaagcaataa aatagtattt taaaaaacat ataaaatagt t
//
LOCUS       DAHHZJ010000288         1106 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.299, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000288 DAHHZJ010000000
VERSION     DAHHZJ010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.299"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            1..999
                     /locus_tag="NBL02_002880"
                     /pseudo
     CDS             1..999
                     /locus_tag="NBL02_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          391 a          199 c          222 g          294 t
ORIGIN      
        1 agaaaaagat aatcttacgc ctgaacaaaa agaattgtta gaactacgta aaagaaacca
       61 gcaattagaa atggaaaatg atattttaaa gcaagcagcg ctgatattcg gacgaagaga
      121 caagtaatcg atgcgaataa gcatctttac cctatatcag cgatgtgcag aatattaggt
      181 ctatcacgtc agtcctatta ttatcaatca aaaccaaaga aagacgaatc agaacttgaa
      241 gaagtagtcg ctgaagaatt tatccgcagc cgaaaggcct acggctcaag aaaaataaaa
      301 aaagccttat caaaacgagg cattcagatc agccgacgaa aaattagtag aatcatgaaa
      361 aatagaggat taaaatcgag ctatactgtt gcttatttta aagtacatca ttctacttgc
      421 aatgaagcca aaacgacaaa cgtattgaat cgtaaattct taagagacaa cccattagaa
      481 gcgatcgtaa cagacttgac ttatgtacga gtcgggaaaa aatggaatta tgtctgtttc
      541 attttggatc tgttcaatcg agaaattctc ggctattctt gtggagaaca taaagatgcc
      601 gttctagtaa aaaaagcatt tagccgtatc aaacaacctc tgacagaggt tgagattttt
      661 catactgatc gtggaaaaga gtttgataac caagctattg atgaattatt aacaactttt
      721 gacatcaatc gatcattgag tcataaaggc tgtccttttg ataatgccgt agctgaatca
      781 acttataagt cgttgaaagt agaatttgtc tatcaataca catttgaaac cttacaacaa
      841 ttggatttgg agttatttga ctatgtcaat tggtggaacc accttcggtt gcacggtaca
      901 cttggctacg agacaccggt tggttaccgt aaccagagat tggcgcagcg aatccttgat
      961 aatgagctcg gatgtgctaa cgctagcgag gcagtctaac tttaactgtt agctcctgcc
     1021 gaagatcgtc acatccgagg aggctcattg tcaaggacaa tcggaatagc atacggaaag
     1081 aagttctgca ccttataaaa tttgtc
//
LOCUS       DAHHZJ010000289         1102 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.300, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000289 DAHHZJ010000000
VERSION     DAHHZJ010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.300"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(62..280)
                     /locus_tag="NBL02_002881"
                     /pseudo
     CDS             complement(62..280)
                     /locus_tag="NBL02_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(546..740)
                     /locus_tag="NBL02_002882"
     CDS             complement(546..740)
                     /locus_tag="NBL02_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573502.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(840..926)
                     /locus_tag="NBL02_002883"
                     /pseudo
     CDS             complement(840..926)
                     /locus_tag="NBL02_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          385 a          187 c          158 g          372 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattata ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt agtacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa at
//
LOCUS       DAHHZJ010000290         1079 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.301, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000290 DAHHZJ010000000
VERSION     DAHHZJ010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.301"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(680..973)
                     /locus_tag="NBL02_002884"
     CDS             complement(680..973)
                     /locus_tag="NBL02_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HCD2573503.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT          334 a          176 c          162 g          407 t
ORIGIN      
        1 aaaatactct ttttttacga ttattgaaaa aaaaagataa aacttgatta attgaagtat
       61 tgcttaaatt tggaagctat gagggaagaa taaaagagtt aggacgggat tatctactat
      121 ttttctttgt tttaaatatt tgttatcttt gaacataaaa gagaaatgtg tcgactgcag
      181 tgttgtgtcc ctcatcacga ataaacggtg ttcaactagc tgatcttgta ttcacttaaa
      241 aaatggtaaa gtacgagttg ctattcccca ttttcaattt tctaacgctc aggacaaaat
      301 actgttttca caacctcatt ggaataaaag catatatatt gtttcaagaa cgaaatcttg
      361 ttatcttttt aaagggcatc tgtccaaaat ctaattttag gaggtacgtt ttggtattta
      421 ttcgtacaaa taaaagagaa ttatctttat tcatgtaacg catccaattg attttttcat
      481 gtacgctcct ggaacgattt tactgacgat tgcttattta actgcgaatc gctcattagc
      541 ttttatcata gctatccata ttttgaataa cgttcttggc tttgtattat agatacagat
      601 gacaaaaaaa gttcaagtat gcggtgtaga accaatatcg catacttgaa cttttttcaa
      661 tgagtataaa ttaaaacttc taattttcaa ttcccatcat ctgatatgcc cgaccgtctg
      721 aacttctttt gaaaaagcca aatgtgatta gataacgtct ttgttcgaca taatcgccac
      781 tagaaaatac gttcagcagt atcgaattaa cctctgtttc tgtataagtc actccatggt
      841 caaagcgttt caagacttct ttcaatagga tgtatttctt ttttgttgaa cttggtactt
      901 gttttacttt tccatcattc atgaatgtgt ctaaaacttt catcttttct tgctccgtat
      961 aagtataaac catcttcttt ctccttttga ttctaagaat actccattta tgttagaata
     1021 ttctggattt tttattaaag cggttactct taagaaagat attatcatag tgctctttt
//
LOCUS       DAHHZJ010000291         1035 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.302, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000291 DAHHZJ010000000
VERSION     DAHHZJ010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.302"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(77..307)
                     /locus_tag="NBL02_002885"
     CDS             complement(77..307)
                     /locus_tag="NBL02_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573504.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(401..739)
                     /locus_tag="NBL02_002886"
     CDS             complement(401..739)
                     /locus_tag="NBL02_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573505.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
BASE COUNT          336 a          208 c          111 g          380 t
ORIGIN      
        1 tagtaatatt aacagcaaag atatcgaaaa aacaataaca taatatgtgt caaaattttt
       61 cataataaga actcccttac tgttgcatac taacataatc caaaaactct tctacttcag
      121 ctaaagacat tccactttct ttagtccaaa aaacagtttt ttctatcgat ccttctctgt
      181 aaactgtaaa tgtcggagtc gagttaattg agtagcgact cttgaattct ttccactcat
      241 tttcatcttc tctaactttt gctacattca cgtaataaat attttctttt cccttttcta
      301 ccagcatatc gaatagcttc ctgcttttga tgatcgattt cttgtgctac atctagtaaa
      361 ttttcatcaa tttctatatc ataaagtatt tcactagtgt ctacatccac aatatatact
      421 ttttatcagt agcaattttt gagtaatccg ttaaaatgat ctcagtctcg tcactttcat
      481 cagtataaat atacgttaac aatgaactca ccttttcaat cggtgttagc tctaagccta
      541 ctgtattttt tactattgag tcattattta ccaccataac ttcgtactgt atataccatc
      601 ctatttggtt tccatcaata tcagtaattg gctcactagc taatggagtc tccggaacaa
      661 tattttcctg atatttctta aactcttctg tagcacgaaa aaggttatca tgttcctcac
      721 ttgaaactgg ttgagtcata gaaaatgtta cttggctaaa cagagggaaa ataaacacta
      781 caatcaacaa agtataaaaa aaagcccttc tttttcattt tttatcttcc aatcacaaat
      841 tgaaaaactt attcctgcaa ctttgagtct agtatcacaa tcgactgact acaactattc
      901 atttacattc tactttaagt tcctccttaa ccattaattt tattaaagat acttgtaata
      961 gtgattctca caaaatttca tgaaaaatac tttattactg tatctaaaat tccaatacac
     1021 aacttatcga ctatt
//
LOCUS       DAHHZJ010000292          996 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.303, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000292 DAHHZJ010000000
VERSION     DAHHZJ010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.303"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            262..963
                     /locus_tag="NBL02_002887"
     CDS             262..963
                     /locus_tag="NBL02_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCD2573506.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT          363 a          164 c          156 g          313 t
ORIGIN      
        1 gaaaaaattt ttttgaataa attaaaaaaa taaattatat tatcgttgtt agcgtttact
       61 atatgtatta ttgttgtttt ttatacacaa atcattcgct tcaaaacaaa ttattcaata
      121 ccattgtata ttttttacat aataacaaat tatatttgta aagtattaaa acttgtttct
      181 aatgttggta tgttaaaact ttacaaattc tttaaaaaaa gtgacaataa atatagtgaa
      241 caataaggag aggacttttt aatgcgtgtt aataaaatcg attttcactt ttcaaaactc
      301 agatatcaac ctgattttga acatttctcg gatgaagagt tccaacagat caaggaacat
      361 actgttcttc gttcttataa aaaaggacag atccttttcg acgatggtga tcccagacaa
      421 cggttgtata tcttgaccaa aggactcgtt cgtttagaac gttatgatga aagtgcaact
      481 tattactact atgactatgt gaatacaaat aacctttttc ctatgtcagg aatgtttgga
      541 gaagagacct acacttatac agctcaagcg atgactgata ttgaaacatt ctaccttcct
      601 gtttcctact atgaaaaatt agcacgaaaa aatcccgaac agttgatcta tatgatacaa
      661 aaaatttcta aagtagttga aatacatgaa ttgcgtatcc aaatcggatt gacctctagc
      721 gcctttgacc gggtaaaaca aaatttattc attttgaaag aagaattggg cgtaccaaca
      781 aaagatggtc atatcaccat tccttatccg atcaccttaa aagaattagc agtgaatagc
      841 ggcacgacac gtgaaactgc tggacaagta atcaaacaac tacgtgattc cgggctgtta
      901 gattatgaca aaaagcgttt tactttctgt aagacagcta ctactccagc tatgagagaa
      961 taacgcgaac aaaaataagc agtgaccctg ataaat
//
LOCUS       DAHHZJ010000293          988 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.304, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000293 DAHHZJ010000000
VERSION     DAHHZJ010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.304"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            152..385
                     /locus_tag="NBL02_002888"
     CDS             152..385
                     /locus_tag="NBL02_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HCD2573507.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            382..789
                     /locus_tag="NBL02_002889"
     CDS             382..789
                     /locus_tag="NBL02_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HCD2573508.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
BASE COUNT          369 a          134 c          169 g          316 t
ORIGIN      
        1 gaagggagca atctaaattt tttgaatttt cttaattaat tatatagaaa gaacaatctt
       61 tctatagagt tagacatgag agggactatg cttgatttaa aatcaacaag cgtatatact
      121 gtatatacaa agaaaaagga gggagaagac tatggcaatt aaagaaagaa aatttaggga
      181 agtggggaac tcagtagtat taactatttc aaaggagttt cttaaagaaa atgggttaaa
      241 tcccggagat acggtttttt tagatgaaga aaaacttcga gcggcaatta caaaaaaaca
      301 agccttatct gatgaaattg atttagcaat atctcaagca ttaactgaat atgatactac
      361 ttttagagag cttgtcgata aatgagtatt cattacctag aagccaaaga tattataaaa
      421 atgaatgtaa tccaaataaa aaaatactct cctaatgaac cccttggtgt aaaggatgcg
      481 aatgctttag aaatgtgtgt agggcaactt cgagcctcag cttttggtga agaggtttat
      541 ccttcgattt atgaaaaagc agctatccta cttatacaat tgataaagaa acatcctttt
      601 cacaatgcaa ataaacgtac tgctttctta gctacttttg tctttttgaa aatcaatgga
      661 cagcttttag ctattgatca aaaagatgct gtaaatttag cggtatatat tgctacgtat
      721 gataaggatt tcgatcaact aaaatatgaa gtgatcaaca ctataaagac acatacgcag
      781 cccatttaag gagttactgt atgtgtcttt atagggttat tagctgctat agcgaaataa
      841 ttgaagtaaa ttacaaatag ctataattca aatccaacta tatagaaaac gtaaattttc
      901 tatatagttg gattaagata acgactattt attttatcat atttttacta taccctatac
      961 ttttacttgg tatatttatg aataatcg
//
LOCUS       DAHHZJ010000294          971 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.305, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000294 DAHHZJ010000000
VERSION     DAHHZJ010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.305"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(31..104)
                     /locus_tag="NBL02_002890"
     tRNA            complement(31..104)
                     /locus_tag="NBL02_002890"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(68..70),aa:Ile,seq:cat)
     gene            complement(659..886)
                     /locus_tag="NBL02_002891"
     CDS             complement(659..886)
                     /locus_tag="NBL02_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573509.1"
                     /translation="MGFRTKRSTSSEKKWVYSQTIFYGGVISISLLSSTLYSFNVIDV
                     SMSNFISIIGILISAIITQLLLVFEEKSKNN"
BASE COUNT          358 a          152 c          153 g          308 t
ORIGIN      
        1 aacacagaac gattcaattg gctacattaa tgtaccctgt aggactcgaa cctacgaccg
       61 gacggttatg agccgtctgc tctgaccaac tgagctaagg gtagtggttg ttgccacata
      121 aagccataaa caatcaacca gtagaatgcg tggcaacaaa cctgttatcg catatcttgg
      181 agtgtgacta tttatgagtg atagtgaaga tatgcgtctc catatatgta tggttgagac
      241 agataaacat gtatgaaaaa cgttgttaaa tcaacgtttt tttattttgg tcaaggagag
      301 aaaagtagag aaatacaaaa ttttttgcat tgattttgca ttcaaaaaaa cattcaaaca
      361 tttagaaatc cccaatacgt attcaggaaa atctatttta aggtttacaa aaagaacttt
      421 tgttcgtaca ctctttttga ggtgaacttt tatgctgatg gaaggaaaaa cacaattatg
      481 gtttaagcat gttccttcaa ataagttttt taattataaa ctctagagaa attaattcat
      541 cctaacataa aataattgtt cactcaaaag tttttctaga acaattaaaa cagcaggctt
      601 cacattcgct atacatttgt aagaagtaat aattcctgta ctaaaaggca taattgcttt
      661 aattattttt tgacttctct tcaaacacaa gaagtagttg tgtaataatt gcagaaataa
      721 ggattccaat aatagatata aaatttgaca tggaaacgtc tataacatta aaagaataaa
      781 gtgttgaaga aagtaaactg attgaaataa cccctccgta aaaaatagtc tgtgaatata
      841 cccacttttt ttcagaagac gtacttcgtt tggttctaaa acctatccct gcatttctac
      901 tcttatcaaa aaaccaaata tttagaagta ataatattgc tattattaag ataaaattac
      961 tgtaaaaatc c
//
LOCUS       DAHHZJ010000295          961 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.306, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000295 DAHHZJ010000000
VERSION     DAHHZJ010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.306"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(1..961)
                     /locus_tag="NBL02_002892"
                     /pseudo
     CDS             complement(1..961)
                     /locus_tag="NBL02_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          246 a          198 c          176 g          341 t
ORIGIN      
        1 tggacaagac tttgccagtt taatttctgt tttatccgtt cattattgta gtaatagata
       61 tagctatcaa tggctgattt taattcctcg aaacagttat aaactttccc gtgaaaaatc
      121 tcttgtttta ataagccaaa aaaattctcc ataagtgaat tatctaagca gttccctttc
      181 cgagacatgc tttgaaagat tttgtactcc ttcaatttgt ttctataagc tttcatttga
      241 tacgcccaac cttgatctaa gtgaaacata cgacgatagg gacaatcgtt agtggtctgg
      301 acagcttcct ccaaaccttc cattaccgct aatgcattgg gcttttcaga gatacgatag
      361 gataaaatct cactattgaa catatccata aaaggatcta gatacaattt cttttgacgg
      421 ataatgcctt tggcatccgc ttcatggtat ttaaattcag ttgtatccgt cgtgattttc
      481 tgatggcaga tactggtgta gaaacgacga tggatccgat ttttggctac tgctccaact
      541 tgtcctcgat aagaattgta gcgacgtgat ttacgtgtgt atgccgttac ttttattcct
      601 agcttcttga tcaggcgctg aactttcttt ttattgatag agaaaccgcg gtttcttagt
      661 tcattggtca tccgtaaaca gccataatcc ttgtgcttct cccgaatttt aagcatttcc
      721 gcttcaatct cttgatctgg atttttacgg tccaatcgtt tttgccagta cataaaggtt
      781 gccttaggga actttaaagt ttctagaagc tcaactaatt taaacggtcc tcggaggctt
      841 gcgatgattc gtgcgattgc ttcattcgtc gttgttgggc ttcctgtttt cgcagcttcc
      901 tcaattcttt taaaaaggca ttttcaattt gaagggaacg gactcgtttc tccaactcct
      961 c
//
LOCUS       DAHHZJ010000296          956 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.307, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000296 DAHHZJ010000000
VERSION     DAHHZJ010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.307"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            82..495
                     /locus_tag="NBL02_002893"
     CDS             82..495
                     /locus_tag="NBL02_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HCD2573510.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
BASE COUNT          323 a          143 c          162 g          328 t
ORIGIN      
        1 ttatacttgc atttttttca aaatagagtt attacgtaaa tagggataaa taaaaaatac
       61 ttattgaaag gatgatcaaa aatggcaaat gtacctagca ttcgcgacat gttccctgat
      121 ttcaatgacg tgtttagtcc agcattcaat gattttttag gtgtttcaag ttatccaaaa
      181 gtagatttag tcgaaaatga aaaagaatac aaactaactg ctgatatgcc tggctgtgac
      241 aaagaagaca cgacagttga atattcagat aatactttga cgatctccgc caatcatgag
      301 tcccatacag aagataaaga agatggcaac tatgtacgga aagaaagaca ttcggtctct
      361 tacaaacgtt cattctatct tcctaatgtt gatgaagaaa aaatcactgg aacctttaaa
      421 aacggtgtgc tgaaattggt attacctaaa acagcttatc agccaaaaga aacaaaaaaa
      481 attgagttaa attagaactt atcgcctctc gatgaatgag gggcgatttt tatatatttg
      541 gataagaacg agtatgttta aattttcaaa atgagcaaaa aaatattttg tttttacgat
      601 aaagatgtta tgatctctct tggtaaaaaa tatgggagtt gatcatttga ttgtcttaag
      661 taaacttttg ttttggatac catttatcat aatgaccagt gctttctttt acttattcaa
      721 aaggcctaac aaatacttac tcttatctgc ttgtccgatg atctatttta tccataagat
      781 tagaggattt tattattacg actctggtat ctcattgttt tgctacgaaa tcggcttatt
      841 agcttcggcc atctttttca tatttgtgtt agaatatttc agaaaaaaat aaacttccag
      901 tcagttttgg ctggaagttt tttatttggt gcagatcttt tcagctgtta ttcata
//
LOCUS       DAHHZJ010000297          951 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.308, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000297 DAHHZJ010000000
VERSION     DAHHZJ010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.308"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            1..500
                     /locus_tag="NBL02_002894"
                     /pseudo
     CDS             1..500
                     /locus_tag="NBL02_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177099.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            558..>951
                     /locus_tag="NBL02_002895"
     CDS             558..>951
                     /locus_tag="NBL02_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573511.1"
                     /translation="MIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLSFGIG
                     LTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLNIIQAPWRF
                     MIFSTLFISFSVSLFISPQLEKFSYTRRR"
BASE COUNT          301 a          137 c          128 g          385 t
ORIGIN      
        1 tttttagctc ccctattaat tttgaaacgt ttagatcata tggcatgcaa gtaaataatt
       61 tttacccatg gttaactcta tatcctttat ttttattgat caagtttact aatttagcaa
      121 taggctataa cctttttctt tatattgtta cgctcattac tctatttata tgtcattatg
      181 taatgtatga aattaccaaa aaacatgtta ctagctcctt tttcgctatt atatatacaa
      241 catcttcttt taggagtgta gaaatatttc ttcgaggggc aatgggggaa ttactcgcaa
      301 tgtcaatact tcctcttatt ttactcgggt ttataaagct ttacgactca aaaaaagaat
      361 cttgggttat gttggcgatt agcatgactt tattaattta cactcatgtt ctttctgtag
      421 ctatgactat ggtaatgatt ataattgctt taattattca attttatcgg aaaaagaaaa
      481 tagaaatctt cttaattatt aaattaataa aagctgctat agttacttta cttctttcac
      541 tagcgtttat aggtcctatg attgagcaga cattgtatca agatctgaat agaccttatg
      601 tggatatatt acaaaaaaga gcaatttatc tgggtaaaga gtttttgatt ggaagtatag
      661 attcagaatt actatcattt ggtattggct taactttgct tatttgttta attatactaa
      721 catttaactt taaaaaatta ggaatcttat ctaaaatcac ttatataatg ggtataatat
      781 ctattctact ttgtacaaaa atatttccat ggtttgcctt acaaaatact cctttaaata
      841 ttattcaagc tccttggcgt tttatgatat tttcaacatt gttcatatct ttttcagtat
      901 ctctatttat ttctccacag ttggaaaaat tttcttatac aaggagaaga a
//
LOCUS       DAHHZJ010000298          950 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.309, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000298 DAHHZJ010000000
VERSION     DAHHZJ010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.309"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            131..448
                     /locus_tag="NBL02_002896"
     CDS             131..448
                     /locus_tag="NBL02_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HCD2573512.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNASIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(539..904)
                     /locus_tag="NBL02_002897"
     CDS             complement(539..904)
                     /locus_tag="NBL02_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573513.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
BASE COUNT          353 a          120 c          161 g          316 t
ORIGIN      
        1 taactatgca ctgtaacgat aatgctgcta aaatatcttt ttacgaaaaa catcacaagg
       61 aaaaacttaa ctatggtatt atctaaacat aattttttcg gtatttaata tttaattagg
      121 aggtaatatt ttgctttttt atagtaattt tatcttaata gtagcgatat tattacttct
      181 gaatatatgg atttttgata ggagtagaaa tgcaagtata ggttttagaa ctaaacgaag
      241 tctgtcttct aaaaaaaatt gggtatactc acaaactatt ttttatggag gaattgtttt
      301 aattagttta ctttcttcaa cactttattc tttgaatatt atagatgttt ccacctcaaa
      361 ttcaatatct attattggaa tcattattgc tgcaattatt acacaactgt ttttagtgtt
      421 tggagagaag aaaagaagta aaaaatagaa aaactagtga tagttttggt gatataagaa
      481 aaacatatat gtataaaaag aagaaagtag cttctctttg tacatatatg gtttgttttt
      541 atggatgccg agctttttta taagataagg ctaccgccca taacgaccca aggatttgtg
      601 gaactaagta aatacctgca agaagcaaga tcagctgggt cggttttaaa ggatgataga
      661 agtaacgcca ttcagtaaaa ttggcgcaaa ataaatagga agtgacagta gatataatat
      721 tggcaatgaa taaagatata atacgcctgt tcctttgaaa ataatatcca gcaaaaagag
      781 ataaaaatat caatgtgatg aacccgctta cggaatgata acgaaaatca agcagtaagg
      841 cgaaatgcac ataaggaaca aaagataaaa gcaacggaag gtttttccag caagttttta
      901 tcatctttca tcaccaccta acaagtgtta ttattttgtt agttttattg
//
LOCUS       DAHHZJ010000299          946 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.310, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000299 DAHHZJ010000000
VERSION     DAHHZJ010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.310"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            246..632
                     /locus_tag="NBL02_002898"
     CDS             246..632
                     /locus_tag="NBL02_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCD2573514.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            726..917
                     /locus_tag="NBL02_002899"
     CDS             726..917
                     /locus_tag="NBL02_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD2573515.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
BASE COUNT          347 a          145 c          149 g          305 t
ORIGIN      
        1 tcaagacatt ttcttagcca taaggacatg tttgatattt aagaagatat atgaattttc
       61 ttttctaagt ataaagataa ctatccatta tcaactaaaa aaacattttt gaaaataaac
      121 atcagcagtc ctatcaatgc atgtgagctt atagaagtta ttcgcagcgc agcataattg
      181 cattagcttc ttttcgtgtt ttttgcctct tttcccttta aatcttaatg gaggaggtgt
      241 gcaatatgtc agataacgac gaacgcgtaa atgcccagtt gattcgattc tttgaaaagg
      301 tcattttgaa caccgctaat acttattata aaaaacaaga agaaattcaa aaacatgaac
      361 aacttgatca actcccacca tatttgttta ctaaagatac tataaaattt aaacaaccga
      421 ttaatgtttt aaatgtgact ctccttgttg aagatataaa attagcaaaa atccttcctt
      481 tgttgaaaga acctgaacaa atttttctta tcgaaaaatt tatctttgat aaaactgata
      541 aagaaattgg aaaggttctg gggattacaa gacagggtgc gacaaattta aaacatcgac
      601 tttacaaaaa aattcaaaaa atgctggttt aatatctgca taataactaa ttttgtatgc
      661 ttctttgctc gttctcctcc ttcccaaaga aatagattat aagtccatgg gaagaaggta
      721 gatttatgaa ttctttaaaa gaagatttca ttttagcaaa ggctggaaat gaagaagctg
      781 ttgaggcaat tctcaaaaga tttagctctt tgattcataa gcaatcctgg agaactggaa
      841 aatatgatca agattgttat caagaatgta tgcttgcaat atatctagct atctcgaaat
      901 tcgaaattaa agaataactc aactaaggga gtctgtgaca taagta
//
LOCUS       DAHHZJ010000300          943 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.311, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000300 DAHHZJ010000000
VERSION     DAHHZJ010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.311"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..>943
                     /locus_tag="NBL02_002900"
     rRNA            <1..>943
                     /locus_tag="NBL02_002900"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DAHHZJ010000301          928 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.312, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000301 DAHHZJ010000000
VERSION     DAHHZJ010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.312"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            404..>928
                     /locus_tag="NBL02_002901"
     CDS             404..>928
                     /locus_tag="NBL02_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCD2573516.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIV"
BASE COUNT          344 a          129 c          162 g          293 t
ORIGIN      
        1 aaaatatcta acagatctta tagataatag aaataatgaa ttaatgagac aaaacattgc
       61 aagattcctt actaaattag tttctgaatg gaaaagtatt atttctcaaa atatggataa
      121 attaagatat taaggatgtt agttttaaaa taaactaatg tccttatttt tgtgttcaca
      181 tttctgttac ctaagaaata cttaaaggag gtgatgtagc cagcaacgac tacaacttga
      241 acccgctatc tacagtttat ttgttgtttc ttttgggtat actctaatgg tacaaatcca
      301 tatttgctta aagcgttgat ttttcagcgg tttttcttta ttgcaaagtg taccctaaga
      361 cctgtatact aatagtaaat agattggata ggaatgatgg caaatgtatt tagcttattt
      421 aagagtttct tccgacgatc aaagtttagc tcggcaacat aagctaattg aaaattggtc
      481 agtcaaaaat aatattgctg aagacgattt aaaaattttc gaggaaaaag tctctggaaa
      541 aaatattgaa aacagaccag agctgcaaga gcttatttct ttcatacgtg aacgagatac
      601 gattattatt cctagtcttg atcgattagc aagaaatagt aaagatacaa aagacttact
      661 tcaattgttt cgtagtaaag gtgcttctat tgaaatatta gacctaccgt cgtttaatgg
      721 ggtaactgac cctgcattaa gagacttgct aacaaattta gtcatagaag tgtttagcta
      781 tgttgctgaa aacgaacgga aaaaaatcaa agagcgtcaa aaacaaggca ttgaaattgc
      841 aaagaaaaat ggtcgttata ctggtaaaaa gattcaatac catgccaatg caacaggaaa
      901 aaacaaattg atttacgatg aaattgtt
//
LOCUS       DAHHZJ010000302          928 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.313, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000302 DAHHZJ010000000
VERSION     DAHHZJ010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.313"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..153
                     /locus_tag="NBL02_002902"
     CDS             <1..153
                     /locus_tag="NBL02_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCD2573517.1"
                     /translation="VGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWY
                     LNIPKR"
     gene            268..>928
                     /locus_tag="NBL02_002903"
     CDS             268..>928
                     /locus_tag="NBL02_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573518.1"
                     /translation="MTAEIGIMNKSGIVLASDSASTIGDSKVYNTAKKLFTLDSMHSV
                     GIMIYGNAEFNGIPWEIIITQYKKSIGSSVFNTLEEYADNFIEFVKTASFIRSEQTEQ
                     EQMIGVFQKIISGLFESIEVDINFLISQGTQIDKDVLVKLLQTKMNTNLSQQSQTFIL
                     DIEKALFLSNYGEILKDILNSISTMEGVSEAISEEIQSYVYEIIIRDDVYSSPTGIVI
                     AG"
BASE COUNT          352 a          117 c          170 g          289 t
ORIGIN      
        1 gttggctatc atgtatcttt taacatgcgc aatttcccta ataagaaagt atacgtcatg
       61 atgcgggcaa cgaatgatcc agaaggaaac actaaaggcg gtgcgcaaga tttccatgac
      121 aaacgttggt atttaaatat tcctaaacga taaaaatagc tcctcgttga ggagcagtac
      181 atagaattga aaattaacgt taaatcaaaa aaatatttac aagatgttta tttaagtaga
      241 taataaaaga aaaaagcagg tgaaattgtg acagcagaaa ttggtattat gaataaaagt
      301 ggaatcgtgt tagcttcaga ttcagcatct acgattggag atagcaaagt atataatact
      361 gcaaaaaaat tatttacttt ggattccatg cattctgtag gtatcatgat atatggtaat
      421 gctgaattta atggtattcc ttgggaaata atcattactc aatataagaa aagcattggt
      481 agttctgttt ttaatacttt ggaagaatac gcggataatt ttatagaatt tgtcaaaaca
      541 gcatctttta ttagaagtga acagaccgag caagaacaaa tgattggggt ttttcaaaaa
      601 ataatttcag gtttatttga gagtatagaa gtagatatta actttttaat aagccaaggt
      661 acacaaatag acaaagatgt cctagtgaag ttattacaaa caaaaatgaa taccaatttg
      721 tctcaacagt cccagacatt cattttagat attgaaaagg cgttatttct tagtaactat
      781 ggagaaatat taaaagatat tcttaatagt atttctacaa tggaaggtgt ttcagaagct
      841 atatcagagg aaattcaaag ttacgtttat gaaattataa ttagagacga tgtttattct
      901 tcccctacag gaatagttat agcaggat
//
LOCUS       DAHHZJ010000303          911 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.314, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000303 DAHHZJ010000000
VERSION     DAHHZJ010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.314"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(36..>911)
                     /locus_tag="NBL02_002904"
     CDS             complement(36..>911)
                     /locus_tag="NBL02_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573519.1"
                     /translation="YEKKKLFHFLAINLLAEQADSLDIASNQSLFFSDIGFYHLKGVL
                     GEKSVIFINKTLSWLYLHDKLDQKYLRKNEDETIEWLNERFIESFENQFNLLREETSQ
                     LIRRELTKIHFKLLHYTINKADEFPLDVSFFRENYPAFYCYLIEYINDLTSKRKELLQ
                     DKFFLFFSYLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEVIE
                     AGKPEFPDVIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKID
                     KQIDDFPEEIIDRIE"
BASE COUNT          293 a          157 c          139 g          322 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat a
//
LOCUS       DAHHZJ010000304          902 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.315, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000304 DAHHZJ010000000
VERSION     DAHHZJ010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.315"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            177..>902
                     /locus_tag="NBL02_002905"
     CDS             177..>902
                     /locus_tag="NBL02_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HCD2573520.1"
                     /translation="MSKQCAKCGGKIGLTSYKIKDKQIVCGNCMKKAGYGMTTPYKII
                     RSLNLEDLDKGPSEKRKLNISDYTTQNNHDIGNDRMRTTEEMVEFCLKYGYGKGMTKK
                     WTTHHFNLISDQLNSDEYVIFCFVGLHNYISSTKHDNNYAYALTNKRLIVAQQKMIGN
                     NVQSIILDNLNNISKKREILLGTLTIDTLGKVLNVAVDKDTVDRISDSLNEIIYNLKR
                     RNISAINSNTSTISSASEIRKYKE"
BASE COUNT          363 a          111 c          140 g          288 t
ORIGIN      
        1 aattatactg tttcctgata agctctcaat gcttctgaac ccatgatatt agtgcttttc
       61 ttctattatt aaatatcttg taattttcta ttctttctaa aaagtatggt caaagtatgg
      121 tcactattga tagaatatta atagtagtag aaagctgata tctaggagga actaatatga
      181 gtaaacaatg tgcaaagtgc ggaggtaaaa ttggcttaac ttcatataaa atcaaagata
      241 aacaaattgt ttgcggcaat tgtatgaaaa aagctggtta cggtatgact actccttata
      301 aaataataag atctttgaat ttagaagatt tagataaggg tccatcagaa aaaagaaaat
      361 taaacatatc tgattatact acacaaaaca atcatgatat aggtaatgat agaatgagaa
      421 ctactgaaga gatggtagaa ttttgtctta aatacggcta tggtaaagga atgactaaaa
      481 aatggacaac tcatcatttt aacttaatat cagatcaact taacagtgac gaatacgtaa
      541 tcttctgctt tgtaggattg cataactata tctcgtcaac taagcatgac aataattatg
      601 catacgcatt aactaataaa cgtttaattg tagctcaaca aaaaatgata ggcaataatg
      661 tacaaagtat tatattagac aatttaaata atattagtaa aaaaagagaa atactgttgg
      721 gtacattaac tattgatact cttggtaaag tattgaatgt tgcagttgat aaagatactg
      781 ttgataggat aagtgattct ttaaacgaaa ttatttataa tttaaaaaga agaaatattt
      841 ctgctataaa ttccaataca tcaacaattt cctcagcttc agaaataaga aaatataaag
      901 aa
//
LOCUS       DAHHZJ010000305          888 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.316, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000305 DAHHZJ010000000
VERSION     DAHHZJ010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.316"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(<1..>888)
                     /locus_tag="NBL02_002906"
     rRNA            complement(<1..>888)
                     /locus_tag="NBL02_002906"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          192 a          278 c          205 g          213 t
ORIGIN      
        1 gggaaatctc atcttgaggt gggcttcaca cttagatgct ttcagcgttt atcccttccc
       61 tacatagcta cccagcaatg cccttggcag aacaactggt acaccagcgg taagtccatc
      121 ccggtcctct cgtactaagg acagctcctc tcaaatttcc aacgcccgcg acggataggg
      181 accgaactgt ctcacgacgt tctgaaccca gctcgcgtgc cgctttaatg ggcgaacagc
      241 ccaacccttg ggaccgacta cagccccagg atgcgacgag ccgacatcga ggtgccaaac
      301 ctccccgtcg atgtggactc ttgggggaga taagcctgtt atccccaggg tagcttttat
      361 ccgttgagcg atggcccttc catgcggaac caccggatca ctaagcccga ctttcgtccc
      421 tgctcgactt gttggtctcg cagtcaagct cccttctgcc tttacactct tcgaatgatt
      481 tccaaccatt ctgagggaac ctttgggcgc ctccgttacc ttttaggagg cgaccgcccc
      541 agtcaaactg cccatctgac actgtctccc accacgatta gtggtgcggg ttagagtggc
      601 cataacgcag gggtagtatc ccaccagcgc ctccatcgaa actagcgttc cgatttctac
      661 ggctcctacc tatcctgtac atgcggtaca gacactcaat atcaaactac agtaaagctc
      721 catggggtct ttccgtcctg tcgcgggtaa cctgcatctt cacaggtact aaaatttcac
      781 cgagtctctc gttgagacag tgcccaaatc gttacgcctt tcgtgcgggt cggaacttac
      841 ccgacaagga atttcgctac cttaggaccg ttatagttac ggccgccg
//
LOCUS       DAHHZJ010000306          862 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.318, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000306 DAHHZJ010000000
VERSION     DAHHZJ010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.318"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(49..121)
                     /locus_tag="NBL02_002907"
     tRNA            complement(49..121)
                     /locus_tag="NBL02_002907"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(86..88),aa:His,seq:gtg)
     gene            complement(124..197)
                     /locus_tag="NBL02_002908"
     tRNA            complement(124..197)
                     /locus_tag="NBL02_002908"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(163..165),aa:Trp,seq:cca)
     gene            complement(204..284)
                     /locus_tag="NBL02_002909"
     tRNA            complement(204..284)
                     /locus_tag="NBL02_002909"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(248..250),aa:Tyr,seq:gta)
     gene            complement(286..361)
                     /locus_tag="NBL02_002910"
     tRNA            complement(286..361)
                     /locus_tag="NBL02_002910"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(326..328),aa:Phe,seq:gaa)
     gene            complement(397..469)
                     /locus_tag="NBL02_002911"
     tRNA            complement(397..469)
                     /locus_tag="NBL02_002911"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(434..436),aa:Asp,seq:gtc)
     gene            complement(479..551)
                     /locus_tag="NBL02_002912"
     tRNA            complement(479..551)
                     /locus_tag="NBL02_002912"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(516..518),aa:Val,seq:tac)
     gene            complement(567..638)
                     /locus_tag="NBL02_002913"
     tRNA            complement(567..638)
                     /locus_tag="NBL02_002913"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(603..605),aa:Glu,seq:ttc)
     gene            complement(653..742)
                     /locus_tag="NBL02_002914"
     tRNA            complement(653..742)
                     /locus_tag="NBL02_002914"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(706..708),aa:Ser,seq:gga)
     gene            complement(782..855)
                     /locus_tag="NBL02_002915"
     tRNA            complement(782..855)
                     /locus_tag="NBL02_002915"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(819..821),aa:Asn,seq:gtt)
BASE COUNT          240 a          238 c          195 g          189 t
ORIGIN      
        1 gtcaaagtcc cttgccttac cgcttggcta taccccaaga ataaaaaggg cgaatgatgg
       61 gaatcgaacc cacgaatgcc ggagccacaa tccggtgcgt taaccacttc gccacattcg
      121 ccatggcagg ggcagcagga attgaaccca cactaacggt tttggagacc gctgttctac
      181 ctttaaacta tgcccctata aaatggagga gagtggattc gaaccactga accctaagga
      241 acggatttac agtccgtcgc gtttagccac ttcgctactc ctccatggtg gcgcgggaca
      301 gaatcgaact gccgacacac ggagcttcaa tccgttgctc taccaactga gctaccgcgc
      361 catattttaa aattattaat tgaataacaa gaacgacggt tccgacggga atcgaacccg
      421 cgatctcctg cgtgacaggc aggcatgtta acccctacac cacggaacca gttcattatg
      481 gaggttaacg ggatcgaacc gctgaccctc tgcttgtaag gcagatgctc tcccagctga
      541 gctaaacctc caacattcaa ttaaaatgac ccgtacggga ttcgaacccg tgttaccgcc
      601 gtgaaagggc ggtgtcttaa ccgcttgacc aacgggccag gtaaatttgc tacggagagt
      661 aagggattcg aacccttgag acagtttggc accatctaca tgatttccaa tcatgctcct
      721 tcggcctctc ggacaactct ccagagtgaa agaagctcaa tactctttct caaaatgaga
      781 actccggcag taggactcga acctacgaca tcatgattaa cagtcatgcg ctactaccaa
      841 ctgagctatg ccggaataac at
//
LOCUS       DAHHZJ010000307          861 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.319, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000307 DAHHZJ010000000
VERSION     DAHHZJ010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.319"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(<1..>861)
                     /locus_tag="NBL02_002916"
     CDS             complement(<1..>861)
                     /locus_tag="NBL02_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCD2573521.1"
                     /translation="SDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKC
                     KATVMYPPNGLPLIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNP
                     ELLSAVLFGYKKGAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQK
                     YRKLGESVQWEYANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLL
                     FEFLKDEAITIDSKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSI
                     YLGDKLDSGF"
BASE COUNT          272 a          162 c          123 g          304 t
ORIGIN      
        1 caaaaaccgc tgtctagttt gtcccctaaa taaattgaag actttctctt atttcctaaa
       61 taaccttctg cacaaagaag cttgatttca ttcctcaatt ctccaacatt tccttcaaat
      121 gttttcgata aaagatcagt aaaaagttca atcgaacaaa atatttttga atcaattgta
      181 attgcttcat ctttaagaaa ttcaaataat aattgaattt tttcagatgt acttctatta
      241 ttgtaatctg gcagagtgat ttcagctggg atgcgtcttc taaaagttgc taacaaagag
      301 gtatttttat cttctgttgt ggctaagata aggcgaacat ttgcatactc ccattgcaca
      361 ctttcaccaa gtttacgata cttttgagaa tccataagaa gaaacaattt ctcttggttt
      421 tctgcactta gattgtgaac ctcatctaaa aaaaggtatc ctccattagc agaagatagc
      481 atccctgcag tatctttttc agccccagta aaggctcctt ttttatatcc aaataaaact
      541 gctgacaata gctctggatt atttgcataa tctgcacaat tcacaacaac aaattttgcg
      601 tcattatgaa ttacttttct atcaagagca tactgataga tcaaacttgc taaaaaactt
      661 tttccaacgc cactgtttcc ttttattatt aatgggaggc cattaggggg atacattaca
      721 gtagctttgc atttatctat agtggatttc aaagatatgt ctgctccaat aaacttagaa
      781 aagcagtctt ttggcttttg ttgattatac ttgaacatta ctggtctaac agaatcattc
      841 tttagtaatt ttccatctga a
//
LOCUS       DAHHZJ010000308          853 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.320, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000308 DAHHZJ010000000
VERSION     DAHHZJ010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.320"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(196..595)
                     /locus_tag="NBL02_002917"
                     /pseudo
     CDS             complement(196..595)
                     /locus_tag="NBL02_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
     gene            complement(592..828)
                     /locus_tag="NBL02_002918"
     CDS             complement(592..828)
                     /locus_tag="NBL02_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCD2573522.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
BASE COUNT          264 a          155 c          121 g          313 t
ORIGIN      
        1 aatcagccca ctagttctaa accactattt cctactattc actcttttgt attcctttta
       61 aatcaattat ttcaaaatta atccatattt tccatatttt tatgaaaaaa ctattttgta
      121 tgattaaaca taaaaaaatc agcatattcc ttaatgacat gctgatctaa ttgagaaata
      181 aagtttttaa ttcgttcatt ttattgcggt ttgtcgtaaa taactggtta tcttggactt
      241 tagtgaatcg aaatccaatg aactagtggt aatcattatg ataaaatcaa cagcttcttg
      301 acgagaaaaa gcaactggac aattattcaa ttgaagaaac aacaacatgg caacaagagc
      361 agttcgttta ttgccattat gaaaaggatg ttttttagct aaattaattg ctaaaatcgc
      421 cgctttttca tagatttcag gatagagttc ttgattgaat actgcttgtg aaggctgttt
      481 gacggccata tctaatgcat ttgcatcttt tacaccgatc atttcgcctg gcgagtgacg
      541 gtaaaataac tttcgcgttg attttgataa tttctttagt cgaaagatac ctcataaatc
      601 aaccaaatct tttaacgcgt cttcatattg attcatgatc gaatcaacta atgaatcaat
      661 atcaggttct tcttgtgctt tgatcattcg tacagaacca tcagattgaa aaacataact
      721 gattgcgtca cctgtctgca cacccatgaa ttcttttact tctggtggga tagttgttac
      781 atcgctattg ccagattttc taattttata ctttttcatt tttttcatat ttcgccaccc
      841 tctataccat tct
//
LOCUS       DAHHZJ010000309          806 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.322, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000309 DAHHZJ010000000
VERSION     DAHHZJ010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.322"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..417
                     /locus_tag="NBL02_002919"
     CDS             <1..417
                     /locus_tag="NBL02_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HCD2573523.1"
                     /translation="DLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGN
                     NRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDI
                     VEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
     gene            complement(547..>806)
                     /locus_tag="NBL02_002920"
     CDS             complement(547..>806)
                     /locus_tag="NBL02_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCD2573524.1"
                     /translation="KYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLIERLNQEVRR
                     REKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          305 a           79 c          146 g          276 t
ORIGIN      
        1 gatttaactg atatagaaaa agattatata gaaagtttta tggaaagact aaatgcaaca
       61 acagtttttg agggtaaaaa gtgtttatgc cataatgatt ttagttgtaa tcatctattg
      121 ttagatggca ataatagatt aactggaata attgattttg gagattctgg aattatagat
      181 gaatattgtg attttatata cttacttgaa gatagtgaag aagaaatagg aacaaatttt
      241 ggagaagata tattaagaat gtatggaaat atagatattg agaaagcaaa agaatatcaa
      301 gatatagttg aagaatatta tcctattgaa actattgttt atggaattaa aaatattaaa
      361 caggaattta tcgaaaatgg tagaaaagaa atttataaaa ggacttataa agattgatta
      421 tataatatat gaaaagctat tataaaagac attagtatta aatagtttaa aaaaatgaaa
      481 aataataaag gaagtgagtc aattccagac tcctgtgtaa aatgctatac aatgttttta
      541 ccatttctac ttatcaaaat tgatgtattt tcttgaagaa taaatccatt catcatgtag
      601 gtccataaga acggctccaa ttaagcgatt ggctgatgtt tgattgggga agatgcgaat
      661 aatcttttct cttctgcgta cttcttgatt cagtcgttca attagattgg tactctttag
      721 tcgattgtgg gaatttcctt gtacggtata ttgaaaggcg tcttcgaatc catcatccaa
      781 tgatgcgcaa gcttttgaat attttg
//
LOCUS       DAHHZJ010000310          802 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.323, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000310 DAHHZJ010000000
VERSION     DAHHZJ010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.323"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..>802
                     /locus_tag="NBL02_002921"
     CDS             <1..>802
                     /locus_tag="NBL02_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HCD2573525.1"
                     /translation="SFSIVHNQVYFGGGINLWLAKKNGIRQRIAHSHATEDGKSQNIV
                     MNVLRKFLTKLLLQNATDYLAVSQEAGESLFQNHPFEIVHNGIDLELYSKNSEAKVNK
                     RKELDISMSTFVVGNIGRLEKQKNQSYLLEIFLEILKMNSDSYLILVGEGSLRNKLKA
                     KAVELDIEKRVKFLGERNDIPELLSVMDVFVMPSLYEGLPMVGLEAQAAKLKLVLADT
                     ISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYANKITQELKSYDVSYTT"
BASE COUNT          305 a           99 c          153 g          245 t
ORIGIN      
        1 atctttttct atagttcaca atcaggttta ttttggcggg ggaattaatt tatggttagc
       61 caaaaagaat ggaatcagac agagaattgc tcatagccat gcaactgaag atggaaaatc
      121 tcaaaatatt gtaatgaatg tattgcgtaa atttttaacc aaattgttgt tacaaaatgc
      181 aacagactac cttgccgtta gtcaagaagc tggtgaaagt ctgtttcaaa accatccctt
      241 tgagatagtt cataatggta ttgatttaga gctttatagt aaaaattcag aagctaaggt
      301 taataagaga aaagaattgg atatctcaat gagtactttt gttgtgggaa acattggacg
      361 attagagaaa caaaaaaatc aatcttattt gttagagatt tttttagaaa tactaaaaat
      421 gaattctgat tcttacttga ttttagtagg tgaagggtca cttagaaata aactgaaagc
      481 caaagcagta gaattggata tagagaaaag agtaaaattt ttaggagaaa gaaacgatat
      541 tcccgagtta ttatctgtga tggatgtttt tgtaatgcct tctctttatg aaggtctgcc
      601 aatggttgga ttagaagcac aagcagcaaa attaaaatta gtcttagcag atactatttc
      661 caaagataca aagttaacaa ataatgtgga ttttgtatca ttagatgaaa caccacaaga
      721 atgggcaaaa aaagtaataa aagagccgta tgctaataaa ataacacaag aactaaagag
      781 ttatgatgtt tcgtacacta ct
//
LOCUS       DAHHZJ010000311          793 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.324, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000311 DAHHZJ010000000
VERSION     DAHHZJ010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.324"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            8..>793
                     /locus_tag="NBL02_002922"
     CDS             8..>793
                     /locus_tag="NBL02_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCD2573526.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYL"
BASE COUNT          270 a           86 c          116 g          321 t
ORIGIN      
        1 attaagaatg ttcgtgggaa actttttctt ttttctctta ttttatatga ctaatactga
       61 tcttaacaaa agaaaatatc tttttgtatg tttattgata ccactcactt tttttgttga
      121 attatttact gatgtttccg cagctatacc tataatcgga agttatttta tattaaagga
      181 aaagaagaaa actaatatag tgcttttaaa taaattacta ttatgtatgc ttattgatga
      241 tttcagatct attattactt ccatggttat gacgtatatg ttttcatttg aagggataaa
      301 agggtatggt tatgtagtat tccagataat gcttgattct ttgtttttgc tgatgtttat
      361 ttggttatat agaaaattag ctataaatag gattgttgaa cagtccagtt ctaaacttac
      421 tgctatattc atgatttatt tacttacaat tggtttattt gtttcttatg ttgctcatca
      481 atatcaagta tttgatcatt ttgtgtttgg tgtcttaata tttttaatca ttcaaatgtt
      541 tgttgttcta tttttattta ttagaataac tattaggcaa cgagaacaat atgaacagca
      601 attgaaaaga aaagaattgg tttatctaaa aaaatatact gattcactgg aaaaagatca
      661 agaaaaattt gccagattta gacatgacta taaaaattta ttgctgagtt taaaagaaat
      721 cgctaatcaa aaccatgatt cgatattgga aaaagaaata aaagaattag agatctattc
      781 taattcttat cta
//
LOCUS       DAHHZJ010000312          792 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.325, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000312 DAHHZJ010000000
VERSION     DAHHZJ010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.325"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..>792
                     /gene="bsh"
                     /locus_tag="NBL02_002923"
     CDS             <1..>792
                     /gene="bsh"
                     /locus_tag="NBL02_002923"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HCD2573527.1"
                     /translation="KLNFRKVNDLDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLN
                     FSGYADYKEIQEGKDNVSPFEFIPWILGQCSTVGEAKKLLKNINLANINYSDELPLSP
                     LHWLLADKEKSIVIESMKDGLHIYDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNN
                     FSNQISLNAYSRGMGGIGLPGDLSSVSRFVKATFTKLNSVSGDSESESISQFFHILGS
                     VEQQKGLCDVGDGKYEYTIYSSCCNVDKGIYYYRTYEDSQITAID"
BASE COUNT          268 a          110 c          146 g          268 t
ORIGIN      
        1 taagttgaat tttcgaaagg taaatgattt ggatactcat tatgcaatga ttggtattgc
       61 cgctggtata gctgactacc ctctttatta cgatgcgaca aatgaaaaag gattgagtat
      121 ggctggacta aatttttctg ggtatgctga ttataaagaa atacaagaag ggaaagacaa
      181 tgtatctcct tttgaattta ttccttggat tttaggacaa tgctcaacag taggagaagc
      241 taaaaaattg ttaaaaaata tcaatttagc aaatataaat tatagtgacg aacttccttt
      301 atccccttta cattggctat tagctgataa agaaaaatca attgtcattg aaagtatgaa
      361 agatggactt catatatatg ataaccctgt gggcgttctt accaataatc cttcatttga
      421 ctatcaatta tttaatttaa acaattatcg tgtcttatcg agtgaaactc ctaaaaataa
      481 tttttcaaat caaataagtt tgaatgccta tagccgcggt atgggaggga taggcttgcc
      541 tggagattta tcctcagtat ctcgttttgt taaagcgact tttacgaagc tgaattctgt
      601 atctggagat tcagagtcag aaagtattag tcaatttttc catatcttag gttcagtaga
      661 acaacaaaaa ggtttgtgtg atgttggtga tggaaaatat gaatatacaa tttattcttc
      721 ttgttgcaat gttgacaaag gaatctatta ttatcgaaca tatgaagaca gtcaaattac
      781 tgcaattgat at
//
LOCUS       DAHHZJ010000313          790 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.326, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000313 DAHHZJ010000000
VERSION     DAHHZJ010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.326"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            29..>790
                     /locus_tag="NBL02_002924"
     CDS             29..>790
                     /locus_tag="NBL02_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCD2573528.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYI"
BASE COUNT          314 a           79 c          150 g          247 t
ORIGIN      
        1 aaaacaaaaa cataaatagg agagaaatat gaataattgg atattttgta ttgattcaga
       61 tggctgtgtt atggatacga tgacttataa acacaatctc tttttcggac caattgctgc
      121 agagattttt gatgttcatc atagagaaaa attcctacag gattggaata atattaattt
      181 atattcagag actcgtggaa taaacagatt tttaggttta gttaaaacat tagaaaaaaa
      241 tgatgtaaca tcgattaatc gattaaagca ctgggtatct ctaacaaaag aattgtctaa
      301 tccatctttg aagagggaaa tagaatattc tcagtcagag gatttattaa aagctttaaa
      361 atggtcggaa gaagtcaatc aacagattag tcaagcaaaa ggattagata aacccttttt
      421 aagtgctaga gatactataa aagctctgaa aaaatatggg aagataatta ttgtaagctc
      481 agctaataag gaagctgttc aagaagaatg ggaaagacat gaattattat cattagttga
      541 tgaactgtgt tgtcaagata aaggaaaaaa agaagatatt attcgaagtg taattgaaaa
      601 tggttgtgat ttggataaga tattaatgat tggtgattct cctggtgatc tagaagctgc
      661 aaataaaaat aaagttttct tttatcctat tttggtcaat aaagaaaaag aatcatggga
      721 aaacttaaga actgatgttt tatttaaatg tttatcggga gattatgtag atattgaaca
      781 aaaatatata
//
LOCUS       DAHHZJ010000314          788 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.327, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000314 DAHHZJ010000000
VERSION     DAHHZJ010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.327"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            460..>788
                     /locus_tag="NBL02_002925"
     CDS             460..>788
                     /locus_tag="NBL02_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
                     /protein_id="HCD2573529.1"
                     /translation="MHGKITASAIARETNKLLNTYVYEAFDMPIEGETSDLINLINNF
                     CKSIDTTNGLILLVDMGSLEQMYEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPI
                     SHFYQMD"
BASE COUNT          288 a           91 c          146 g          263 t
ORIGIN      
        1 aaatcacttt ctgaaattga tttgattgaa ctttttcaaa atcacatttc aattgaagaa
       61 agcatgagtc aattaagaga tagaatactt aaagagtgtg caggttcttc tgagtataat
      121 tatttagaaa gtgacgaatt tatagcatta agaaactatg ttgttaataa aatatcacca
      181 attattgatt cgacaggtct atgtttatta gatgaattct tggcggatat cagtctattt
      241 ataatgttta ttgatgtaat tgaagaaaca tcaagaaatt ttttgctgtc taagttcaga
      301 aaatttcgta tgaaaaatga taaacaaaaa ttattggcaa atgagatttg gagtagttta
      361 gaagtcgaag aaagtaaaca agaacttttg ttgaaatgga tactttttct tgtaaagaag
      421 ttctatgtga aaataccaga aacccattgt ctgatagtta tgcacggtaa aataacagct
      481 tctgcgatcg caagggaaac aaataagttg ctgaatacat atgtttatga ggctttcgat
      541 atgccaattg aaggtgagac tagtgactta attaacctaa tcaacaattt ttgtaaaagt
      601 atagatacga cgaatggact cattctgcta gttgatatgg gatcattgga acaaatgtat
      661 gaaaaaattg aatccaatgt aataggtgat ttggtaattt taaacaatgt ttctacagct
      721 ttagcgattg aatgtgggat tcaagtttgc caaaaaaagc caattagtca tttttaccag
      781 atggattt
//
LOCUS       DAHHZJ010000315          778 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.328, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000315 DAHHZJ010000000
VERSION     DAHHZJ010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.328"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            33..>778
                     /locus_tag="NBL02_002926"
     CDS             33..>778
                     /locus_tag="NBL02_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HCD2573530.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRG
                     VQEVLLFITDGLKGMKDTIHQIYPKAKYQH"
BASE COUNT          282 a          146 c          134 g          216 t
ORIGIN      
        1 gacaaccaat tcacagaaaa gaggacttcc ctatgaatga ttttactaca gaaattgtgc
       61 aaactctagt cactaaaggc gatttaaatg aattattccg ttcgcactta gaaaaagcga
      121 taaacacact cctacggact gaattaacgg cttttttaga ttacgaaaaa tatgatcgca
      181 ctggttttaa ttcaggtaat tcgagaaacg gttcttactt tcgatcaatc aaaaccgaat
      241 atggtgaatt aacattggaa atacctagag atcgtaatgg tgagtttaaa caacaaactt
      301 taccagccta caaaagaaca aacgatacat tggaaaccac tattatccat ttattcgaaa
      361 aaggtgttac gatgtctgaa attgctgatt tgatcgaaaa aatgtacggt catcactata
      421 ctccacaaac catgtccaac atgactaaag ttctgactga agaagtaaat gcctttaaat
      481 ccagagcctt aaatgataag tatgtcgcta tttttatgga cgctacttac attccactaa
      541 aacgtcaaac cgtatccaaa gaagcgattt atattgccat tggtatacga gaagacggca
      601 ctaaagaagt actgagttat gcgattgctc caactgaatc aacatacgtt tggaatgagc
      661 tgctacagga tattaactcc agaggagttc aagaagtctt gctttttatt acggacggct
      721 taaaaggcat gaaagatact atccatcaaa tttatcctaa agcaaaatat cagcattg
//
LOCUS       DAHHZJ010000316          778 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.329, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000316 DAHHZJ010000000
VERSION     DAHHZJ010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.329"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..>778
                     /locus_tag="NBL02_002927"
     CDS             <1..>778
                     /locus_tag="NBL02_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCD2573531.1"
                     /translation="RMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQEYDKV
                     ILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYIGVEIA
                     EAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYLGRKKI
                     EKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKDIYAIG
                     DCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPS"
BASE COUNT          305 a           94 c          139 g          240 t
ORIGIN      
        1 tagaatgaca ctaaattctg agataaaaaa gttatttcta gagacagaag ttgacaaaat
       61 tgatattcaa aataaaaaga ttagttgtgt aaaaaaagat ggtacaagat tctctcaaga
      121 atatgacaaa gttatattag ctacaggttc aaagcaaatt atgctagata taccggggaa
      181 taatttagct aatatttttt cgataaagaa ttttccaaat gcaaaagaga tttttaaagc
      241 tttggatgat agtactatta aaaaagtagg aattattgga gcaggatata ttggagttga
      301 aattgctgaa gccataagaa aaagagggaa agaagtatat cttttcgatg ttgcagatag
      361 agtgttgtcg acttattacg accgtagttt ttcagataaa gttgaagaaa tactatcaaa
      421 aaatggaata catctttgtc tatcagaaag tcctaaaaaa tatttaggaa gaaaaaagat
      481 tgaaaaaata gtcactacaa gtggtaccca atactctatg gatatggtcg tacaagctat
      541 aggatttcta tctaatagtc caattgggga aaaggaactc ttgagagatg ttagtggagc
      601 atatctaact aatgaatacc agcaaacaaa tataaaagat atatatgcta ttggggattg
      661 tgctacaact ttttttacgt ccattaatag aagtagtgtt gatttttcta tttctaatgc
      721 tctaagaaca gcttatattg caactcaaca tattaacaat caagatttta caccttct
//
LOCUS       DAHHZJ010000317          773 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.330, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000317 DAHHZJ010000000
VERSION     DAHHZJ010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.330"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(91..>773)
                     /locus_tag="NBL02_002928"
     CDS             complement(91..>773)
                     /locus_tag="NBL02_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HCD2573532.1"
                     /translation="GLQLSRRRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLD
                     RTFTQEQPLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFG
                     RIPYSLTDVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVK
                     VEFVHQYQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERG
                     SDTSGEAA"
BASE COUNT          209 a          183 c          150 g          231 t
ORIGIN      
        1 ttgtgaatgc tgcggtgtta tggctacgat tgcccttgac aatgagcatc ctcggatctg
       61 attttctccg gcagaagcac aatcattcaa ttacgctgcc tctccagagg tatcagatcc
      121 gcgctcatta tcaaggattc gcttcgccaa tctctgttgt cgaatttgga tcggtgtttc
      181 atacgccaac gtgccatgta agcgtagata gttccaccaa tgcacataat caaacaattc
      241 tagacgtagc tgtgccagtg tctcaaattg gtattgatgc acgaattcta ctttgacaga
      301 tttataggtt gattccacaa cggcattatc atacggacaa cccttcctac tcaatgaacg
      361 agtaattcca aaaccattca gaatctcatg aatggtttgg ttatcaaatt cctttccccg
      421 gtctgtatga aaaagcttga cgtctgttaa agaatacggt atccgtccaa aggcttcttt
      481 taccaatgag gcatctttct tctcaccaca ggaataacca ataatttctc gattaaacaa
      541 atcaagtatt aagcagatat aatgccactt tttccccacg cgaacataag taagatccgt
      601 aacgatggct tccaatggct gttcttgtgt aaaggtccga tctaatacat tcgctgtttt
      661 cgcttcatta caagctgttc gttgcccttt aaaatgagcg atcgtatagg tagatgtcaa
      721 tccgcggcgt ttcatgattc gaccgattcg gcgccgactg agttgaagcc cac
//
LOCUS       DAHHZJ010000318          764 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.331, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000318 DAHHZJ010000000
VERSION     DAHHZJ010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.331"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(133..717)
                     /locus_tag="NBL02_002929"
     CDS             complement(133..717)
                     /locus_tag="NBL02_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCD2573533.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          249 a          170 c          103 g          242 t
ORIGIN      
        1 taatattaac aaatgaataa aatccttaca acatttgaga ataagttatc agtagacact
       61 tacaaaaaat tctcatacta ttgtcacttt tctagataaa tggcaaaaat tttcagcatt
      121 aaatgacaaa aactaatgag aatttgtctt tccatttttc tcctccattt ttgccaaata
      181 acgataaatg gtggttcttg agacgttcca ccgttccgca atagtcttaa taggtgtacc
      241 gctatccact aaagtcttca gtaattccaa atcttgttca gacaattttt caggacgacc
      301 accaaaccgt cctcttgcac gagcagctgc tcgtcctgct gcggatcgtt ccaggatcaa
      361 attacgctca aattctgcaa atgccgcaaa caaatgaaac atgagctgtc ctgtagaact
      421 tgatttgtcc atcgtaatat tttcttgaat actatgaaaa ctcacaccac gctcattcag
      481 tcgattgacg attgaaatca aatcttccat atttctacct aaacgatcta agcgccaaac
      541 gacaagtgta tcgccagagc gaacaaaatc aatggccatt tctaatcctg gtcgattact
      601 ttttgcccca ctcatatgat cagtaaaaat tttttcacaa ccaaatttat ttaggctgtc
      661 ttcttgtaaa tccaaatttt gtaaaccagt agatacccgg gcgtatccta ttttcaaaaa
      721 aatcactcct tcattaattt tattgtaaca taactttatc cata
//
LOCUS       DAHHZJ010000319          763 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.332, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000319 DAHHZJ010000000
VERSION     DAHHZJ010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.332"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            25..426
                     /locus_tag="NBL02_002930"
                     /pseudo
     CDS             25..426
                     /locus_tag="NBL02_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            445..>763
                     /locus_tag="NBL02_002931"
     CDS             445..>763
                     /locus_tag="NBL02_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573534.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASASPVVVDASSTPKTKNDN
                     QLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSELFSS
                     DYDV"
BASE COUNT          285 a          101 c          137 g          240 t
ORIGIN      
        1 aaatgtaaaa attaggagaa atcaatgtct tatagagtac aattaataat ttcagaagat
       61 gtagaaagtc agcaatttgg cactaatgtt attaataaag taattaatcc agctctttct
      121 attaatgcgc ctttaattcc tactgcttta tcattttctg taacagctgt ggtgtctgag
      181 atagaggata cagaaaagat aaaaatagta gaaattgaag ttttaaacaa gaatgaaaaa
      241 caaatttttt ctacgggtga agtatcggta agcttgcctc ctcaagttaa cgatataaac
      301 tttaatataa acgccagaaa tgtcttggtt gaagaagcag gagagcatta tgctgttgtt
      361 aaatttaatg gaactgagat tggaaggcaa atctttgata tcaaggtcaa caaaccagtg
      421 gaaaaaatta aggagacgat acggatgctt gatatagtac catcaaatac acatcggagt
      481 ggtagcttaa taaattttgc tgcgattttt gcttgcgcat cggcatctcc agttgttgtg
      541 gatgcaagtt ctactccgaa aactaaaaat gataatcaac ttgtaattac tacaaaaaat
      601 gagattaaca caatttcaag taatgatggc aaaatttttg atgtgataaa tacggtttta
      661 aaaagaaaag ctgaaatcaa tataaaatat gatgaagatt taaacttgta tttctttgtt
      721 atcaaaacaa catctgaatt gttttcctca gattacgatg ttt
//
LOCUS       DAHHZJ010000320          742 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.333, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000320 DAHHZJ010000000
VERSION     DAHHZJ010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.333"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..390
                     /locus_tag="NBL02_002932"
     CDS             <1..390
                     /locus_tag="NBL02_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCD2573535.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          288 a          128 c          113 g          213 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt
      301 tttgatacat acaatcaaaa atttctaaac agaagccata aaggttttca acaggtaacc
      361 gatacattag tttcaatgtt tactgagtaa ctaattattt tgcaggagga caatttattt
      421 acacaaaatt attgacgctc cctgtttcaa agttagtaga gaaaatgtag ttatatattt
      481 aaatttttac cagaaagaaa tgaacacctt caattatcta aatctcgaaa atggttagat
      541 gtaatcatca ggaaaaacaa tcttaaacga attactattc atggtcttag acacacacat
      601 gctagcttgc ttttagcaag ctagtgcaaa tattaaagat gtgcaagagc gtttaggcca
      661 ctcttctatt caaatcatta tggatctata tattcatatt acagacaaaa gaaaagaaga
      721 aaccgtagcg caattcgcaa aa
//
LOCUS       DAHHZJ010000321          730 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.334, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000321 DAHHZJ010000000
VERSION     DAHHZJ010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.334"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            184..552
                     /locus_tag="NBL02_002933"
     CDS             184..552
                     /locus_tag="NBL02_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HCD2573536.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
BASE COUNT          249 a          121 c          110 g          250 t
ORIGIN      
        1 tttcgtgtaa gaatttcttt gccaagtcaa tcatttttat gtctcttttc tattttatta
       61 tttgagacat cttatcatat aaatatagcg gttacaacat tacttgtgtt ttgttcgttt
      121 tttatacata actattgttt ttctggtaaa ataaatcaaa ccacttaatg gaggcgtttt
      181 agaatggaaa aatttcagcc gatcatcaat caaatcaaag aaaaagaaca tcaacaaaag
      241 atcaaagacc tttttgctaa gataacagaa acgtatcccc agctagacac aactgtaaaa
      301 tggaaccaac ctatgtttac tgaccatggt acctttatta ttgcatttag ttttgctaaa
      361 aatcattttt ctatcgcacc ggaaaaagca gcgattcgtg cccttgaaaa aaatatccaa
      421 gaagcaggct acgtatatac ggataacgtc atcaaagttc cttggaaaag tgtaatcaac
      481 tgggaattga ttgaacaatt gatttcattt aatattgaag ataaaaaagg tcatgataaa
      541 ttttggcgat aaagctttat acgaataaac cttttactgg cgaggaaact attcatcacc
      601 ttatacaggt tttgttcaat cctatccttt ttatcatccc tggttaaaca cttaattgac
      661 aaagtatctg ctatttttat aaggagtctt tcttgtgctg aaaaaaatca ctgattgaat
      721 ggctttgaat
//
LOCUS       DAHHZJ010000322          700 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.336, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000322 DAHHZJ010000000
VERSION     DAHHZJ010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.336"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            91..>700
                     /locus_tag="NBL02_002934"
     CDS             91..>700
                     /locus_tag="NBL02_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573537.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKL"
BASE COUNT          227 a          110 c          158 g          205 t
ORIGIN      
        1 ttttcttttc taattcaacc tgtggtatga tgagagcata agtatcatgt gaaatgatat
       61 gaagtatgtg gaagatggac ggggaagaat atggaagcaa aaatcacttt agaacctttt
      121 gaacgtatct taagcggtta ccgcaaagta gaagagttag ctgtgaacgt aactgattgt
      181 tcaaaattag cgcaaaaata cgcaagattc ggcgttgaag gctatcgatt aggaaactat
      241 gtcggtacag ggtatctgaa ccgttattta gagtgtatgg tggaccgcgc accaatgctg
      301 atttatagac aaaattatct gattccgctg ctttttcgtc gttctgattc agcctttcgg
      361 ttatttgaag aagagtatcg gatggaggct ttctttctac tattagagtg gagcttgaaa
      421 catcgtcctg aaaaaattct tatcgaaaga aatgagaaga tagacacgaa aaaaaataaa
      481 gtgatcgata gtgcatacct agcatttcgt gtatcagaga ttttggattg tggcggctat
      541 ccaatcagca atttccaatc aattgaccaa ttcattgaat ggaatcgaat ttaccgattg
      601 atcgacaatg gcgggattgg ccgtcatagc aaggtatttg acccagaata tccagaaaac
      661 atggaagaac tgaaaatgat tatttcgctt gtcaaattaa
//
LOCUS       DAHHZJ010000323          699 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.337, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000323 DAHHZJ010000000
VERSION     DAHHZJ010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.337"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..195
                     /locus_tag="NBL02_002935"
     CDS             <1..195
                     /locus_tag="NBL02_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573538.1"
                     /translation="DDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            351..>699
                     /locus_tag="NBL02_002936"
     CDS             351..>699
                     /locus_tag="NBL02_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HCD2573539.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          295 a           75 c          141 g          188 t
ORIGIN      
        1 gatgatcaaa gtcctataga taaagaacta aagtatgcta ttttgacatt tgagaaaatg
       61 acagaagaac aaaagaatgc agttatagaa aagatgggcg atggatataa aaattggcaa
      121 aatgagcttg aaagaattgg gactattggt atatttgaaa aaaatacatc agaagaaaaa
      181 aatctaaaat tataaaatgt gatactcata ctaaaatagg agtttcaagt gatggaaaat
      241 acgattgcta tttaagcaca aatagcggag ctgaaagtaa tcttctggat gaattaaaaa
      301 gaactgagat tcaaattata gataaaaaag aacgtcctaa aaatggtttt gtgctatcag
      361 aaaaaactga tttagaaaat acagaagcat ttaacaaaga atcggttaaa gatttacgta
      421 aactaccaac aaaggacata aatggcaagg attttacaag taaagatttt gagaagtatg
      481 atcttactat ggtaaatgta tttgcaactt ggtgtacagc ttgtgtaaag gaaattcctg
      541 atttggttga ggttcagaat gaaatgaaaa gcaagggtgt aaatatcgtt ggagttgtta
      601 cagatcctgt tgatgataac ggtgaaaaca aggaagcaat tgaaaagtca aagttaatac
      661 atgaaaagac aaaggcttca tatcctttct taatgccag
//
LOCUS       DAHHZJ010000324          696 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.338, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000324 DAHHZJ010000000
VERSION     DAHHZJ010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.338"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            35..>696
                     /locus_tag="NBL02_002937"
     CDS             35..>696
                     /locus_tag="NBL02_002937"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD2573540.1"
                     /translation="MNKLLNKELADNIGISEVLLSYVLNRKRNPSKEMQKKIDIFLLS
                     NEKEILKEINRDSEIFSLSKDDKIVMGKRIREVRKNREETLEKFGKNFTIYTGKNVIS
                     RWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDYKNILPKDERVSDFKKINSFSMGLR
                     MRKIRKDYYLEREEFGKLFSPSISKWSIDRYENGRDIPNTNRIIQYAYIGNLSLEFLI
                     YG"
BASE COUNT          279 a           71 c          135 g          211 t
ORIGIN      
        1 gattctcatc ggtaaactaa ttgcatacag agatatgaat aagttactta acaaagagtt
       61 agctgataat ataggaatta gtgaagtatt actcagttat gtgttaaata gaaagagaaa
      121 tccgtcaaaa gaaatgcaaa aaaaaatcga tatttttcta ttaagtaatg aaaaagaaat
      181 tttgaaggag attaatcgtg atagtgagat atttagttta tcaaaagacg ataaaattgt
      241 tatgggaaaa agaatacgag aagtccgaaa gaaccgggaa gagacgcttg aaaaatttgg
      301 taaaaacttt accatctata caggcaaaaa tgttataagt agatgggaaa aaggtattaa
      361 tattccagat attgaaaaac taatgaatat tgcctatctt ggtaaggtca ctgttcctta
      421 ccttatgtac ggagaagatt ataagaatat acttccaaaa gatgaaagag ttagcgattt
      481 taaaaagata aattctttta gtatgggatt aaggatgagg aaaattagga aagattatta
      541 tctagaaagg gaggagtttg gaaagctttt ttctccttca atttcaaaat ggtccataga
      601 taggtatgag aatggtagag atatacccaa tactaatcga attatccaat atgcttatat
      661 aggaaatctt tctttagaat ttttgattta cgggat
//
LOCUS       DAHHZJ010000325          683 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.339, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000325 DAHHZJ010000000
VERSION     DAHHZJ010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.339"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..>683
                     /locus_tag="NBL02_002938"
     CDS             <1..>683
                     /locus_tag="NBL02_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCD2573541.1"
                     /translation="TDESKLILQSHCYFRYMIRTLKELKNYLPKPSKRTLPKVLMVDE
                     FRSHASIVDKMSFICADGETGKLIDILPTRKLSRLTHYFSRCANPEAVEYLVTDMNAA
                     YFQLTKRVLPNAKVVIDCFHIIKHLNQAFNDFRVQEMKRLHQLGQRREAEKVKKNWRF
                     LLKNRQKINHYDYKTWRSFRAPKYPLLTEAMMIDRLLAFSTPLKDAYTFFHELTEAFR
                     DKESDLFFT"
BASE COUNT          218 a          133 c          134 g          198 t
ORIGIN      
        1 gactgatgaa tcaaaattga ttcttcagtc ccattgttat tttaggtata tgattcgtac
       61 attgaaagag ttgaaaaact atcttccgaa gccgtcaaaa aggaccctgc caaaagtatt
      121 gatggtggat gaatttcgtt cgcacgcttc aattgtggat aaaatgagct ttatttgtgc
      181 ggatggcgag actggaaaac tcattgatat tttgcctaca cgaaaattgt ctcgattaac
      241 acactatttt agtcgctgtg caaatcctga agcggtggaa tatttagtga cagatatgaa
      301 cgccgcctac ttccaactga caaaacgtgt cttaccaaat gcgaaagtcg tcattgattg
      361 ttttcatatc atcaaacatt tgaatcaggc gttcaatgat tttcgtgtac aagaaatgaa
      421 aagattacac cagttagggc aacgacggga agccgagaaa gtgaaaaaga actggcgctt
      481 tcttctaaaa aaccgtcaaa aaatcaatca ctatgattac aaaacatggc gcagttttcg
      541 tgcacctaaa tacccgctac tcactgaagc aatgatgatt gatcgattgc tggcattttc
      601 aacgccttta aaggacgcct atactttttt ccatgagctg accgaagcct ttagagacaa
      661 ggaatcagac ttatttttta cct
//
LOCUS       DAHHZJ010000326          677 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.340, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000326 DAHHZJ010000000
VERSION     DAHHZJ010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.340"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(195..593)
                     /gene="spxA"
                     /locus_tag="NBL02_002939"
     CDS             complement(195..593)
                     /gene="spxA"
                     /locus_tag="NBL02_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HCD2573542.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          202 a          138 c          107 g          230 t
ORIGIN      
        1 aatgcgtacg gctttcgcaa cttttacaat ttcaaactac gaattatgat tcgtcatggc
       61 aaaacatttt taaccaaata aacagaacag aaagaggttt tcttctttct gttctgtcca
      121 aattattcta agatttctct ttaccaacat catttgacaa agagccagtc ttttccaagt
      181 ttcgtattat ccttctataa tccagccatc aattgtgctt gacgtaattc taattgtcgt
      241 acatcgcgcg gtaggaaccg acgaatttca tcttcattaa aaccaacttg cagacgttta
      301 tcatcaatca tgattggacg gcgaagcaat ccaggattgt tttgcacaag ttccaataat
      361 tcttgtaacg gcagatcatc taaatccatg ttcagttttt ggaatacttt ggaacgtgtc
      421 gagatgattt cttctgttcc gtcttctgtc atttgtaaaa tcgcttttaa ttcagaactg
      481 ttcaatggtt ctgaaaaaat attacgttca acaaatggaa tctgatgttc ttgcaaccac
      541 gcacgagcct ttctgcagga agtacaactt ggggaagtat ataatgttaa caacgtgcac
      601 cactcctttt cgtgataaga atcacttttt tgtaacagaa gatcataata atctcccatc
      661 tgtttctatt atacata
//
LOCUS       DAHHZJ010000327          677 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.341, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000327 DAHHZJ010000000
VERSION     DAHHZJ010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.341"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(21..251)
                     /locus_tag="NBL02_002940"
     CDS             complement(21..251)
                     /locus_tag="NBL02_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573543.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
BASE COUNT          232 a          100 c           97 g          248 t
ORIGIN      
        1 ctcattaaaa agcataacca ttactccgat ctgtttttaa aattagtttc tacctctaat
       61 atatatcgag tttttatttt gccttcagag aatactgttt ctttttttgc agttacaggt
      121 tgtttatttt cggaaaaagc taatatagct aagattgaaa catccatctg gaatttatct
      181 tttttactgc tttgatcata aaagtctgca tattcatcac tgatattttt tctaataaat
      241 tcttccatca taaaaatcac ctcaaaacga ttatacgaac ttacgttctt gttgtaaagc
      301 gatatttgag gggcaaaaaa ggggcataat ttgactgata ttaagttagc tattgatata
      361 tgaatgacat acaaaaatat aaaatctttt aaaaatgctt atatatcaat gtttttgcca
      421 ttcttttata tatctgttat atgtcaaaaa aatgtgttca atttcaattt gtatttgaat
      481 aaagaggttg tgaaaaagct gtcctgcatc aagtaataag aacagccaat caaaaacagc
      541 ttctcatgtt tttgatttgg ttggacttat taccgcagat ataagctttt gagcaccgtg
      601 aataaagagg ttatgaaatc aacgctctaa cctgagtatt agagaaaaaa acgcaaaaca
      661 tatcatttat gttttgc
//
LOCUS       DAHHZJ010000328          665 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.342, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000328 DAHHZJ010000000
VERSION     DAHHZJ010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.342"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..>665
                     /locus_tag="NBL02_002941"
     CDS             <1..>665
                     /locus_tag="NBL02_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCD2573544.1"
                     /translation="ILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQ
                     PGYVQYGAGSYANANSPVQIELQHTHDKVTFEKNYKAYVELARDSANKYGIPLTLDTP
                     YNQPGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPV
                     IDPTRAGAANPTLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKE
                     LARV"
BASE COUNT          228 a          132 c          139 g          166 t
ORIGIN      
        1 atcctattgc atgaaacagc aaacgaaact gcgacaggac gaaatgaagc gcagtacatg
       61 aaacgttctt ggtacaatgc ctatacagcg tatattgttg gcgatggtgg gatcgtttac
      121 caagttggac aacctggtta tgtacaatat ggcgctggtt catatgctaa tgcaaatagt
      181 cctgtgcaaa tcgaactgca acatacgcac gataaagtaa cgtttgaaaa gaactacaag
      241 gcatatgttg aattagcgag agattccgct aataaatacg gtattccact tacattagac
      301 acaccgtata atcaaccagg aatcaaatca catttatggg taactcaaaa catttggggc
      361 gatcacactg atccgtatgg atacttatca gaaatgggtg taagtaagga aaaactggct
      421 tacgatttag ctcatggatt tacagacgaa aacccaacga cttctgagaa caagcctgtc
      481 attgatccaa cccgagcagg tgcagcaaat ccaactttat cagatggaac gaaccattct
      541 cacattgatc agtttggaga aatcgaaaat gcgaacttac acgtcgctgg atggcacatc
      601 gctaactata aatacgagta tattttcatt atggactaca atactgggaa agaattagct
      661 cgagt
//
LOCUS       DAHHZJ010000329          656 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.343, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000329 DAHHZJ010000000
VERSION     DAHHZJ010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.343"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="NBL02_002942"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="NBL02_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCD2573545.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(481..>656)
                     /locus_tag="NBL02_002943"
     CDS             complement(481..>656)
                     /locus_tag="NBL02_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573546.1"
                     /translation="VPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIYNGINNYI
                     LQAILKELLSNAL"
BASE COUNT          198 a          143 c          111 g          204 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaa
//
LOCUS       DAHHZJ010000330          650 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.344, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000330 DAHHZJ010000000
VERSION     DAHHZJ010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.344"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..>650
                     /locus_tag="NBL02_002944"
     CDS             <1..>650
                     /locus_tag="NBL02_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HCD2573547.1"
                     /translation="ISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHF
                     FDKKQWRYALKISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSV
                     IWMSTNNAWVPWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKD
                     YYSGLSLVVPITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIP"
BASE COUNT          204 a           83 c          123 g          240 t
ORIGIN      
        1 atctctgtag ctttgatgca gacattatat tttgatgata aatatgttgg cagagtagtc
       61 ggcggaacac tgtcatttgt gttgataggt ttagtaattt atttaagaat aatgataaag
      121 ggtgctcatt tttttgataa aaaacagtgg agatacgctt taaaaatctc tttgccgcta
      181 atcccacatt ccttagcaaa tatagtttta gcacaattcg atagaattat gattaattct
      241 tatttaggtc ctgctgcggg gggaatatat tcctacatta gtaacatagg tattattcta
      301 agcgtaatat ggatgtctac taataacgcc tgggttccat ggttctatgg tgagatgagt
      361 aaaaataatg aaaaaaaagt gaagaaagtt agtaactatt atttagtagt ctttactttg
      421 attacgatga ttgtaatggt agtttcgatt gatttagcaa aatttatggc tccgaaagac
      481 tattattctg ggttatcttt agtggttccg ataaccttag gctatttctt tcagttttta
      541 tatagtttac cagttaatgc tgaattttat gagaaaaaaa catcgtttat tgcgattggt
      601 acagtagcaa gtgctatagt taatatacta ttaaacatga ttttcatacc
//
LOCUS       DAHHZJ010000331          645 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.345, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000331 DAHHZJ010000000
VERSION     DAHHZJ010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.345"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(12..>645)
                     /locus_tag="NBL02_002945"
     CDS             complement(12..>645)
                     /locus_tag="NBL02_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCD2573548.1"
                     /translation="SILLVLLVYFSFGTNYAKDFLATIPVSIDRKVEVIESNESDKTP
                     LPEKEVILQHSSDNSEISAGRFTIWKAALKVIRPTLLFGYGETDFYGNSDAKFLKKVD
                     LDTTDVNELKRAHGNMHNGFLQVLVSSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYA
                     FLGIVLIFILSIFIDEMVEAHVLFNKRDVISIVFWYYLGSISYLYLKKEN"
BASE COUNT          241 a          116 c           76 g          212 t
ORIGIN      
        1 ctgtcttcat attaattctc cttttttaaa tacaagtaag aaatgcttcc caaataatac
       61 cagaaaacaa ttgaaatgac atcgcgctta ttgaaaagta catgagcctc caccatttca
      121 tctataaaaa tagatagtat aaaaattaaa acaataccaa gaaacgcata attcacttta
      181 cttctaaaaa atgttttgag taaactaaag atatttaaca catagaaagc atagatacat
      241 ataaaagcaa ttataccact tgatacaagt acttgaagaa atccattatg catgtttcca
      301 tgtgctcttt ttagttcatt cacatctgta gtatcaagat ctactttttt caaaaatttt
      361 gcatcgctat tcccatagaa atcggtctct ccataaccaa ataacaaagt tggtcttata
      421 actttaagcg cagctttcca aatcgtaaaa cgaccagctg aaatttcgga gttatcacta
      481 gaatgctgta atattacttc tttttctgga agtggagttt tatctgattc atttgactct
      541 ataacttcta cttttctatc aatcgataca ggaattgttg ctaaaaaatc cttagcataa
      601 ttagtaccaa aagaaaaata taccaataga actaataata tgctt
//
LOCUS       DAHHZJ010000332          639 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.346, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000332 DAHHZJ010000000
VERSION     DAHHZJ010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.346"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            12..>639
                     /locus_tag="NBL02_002946"
     CDS             12..>639
                     /locus_tag="NBL02_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HCD2573549.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLK
                     IHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          225 a          113 c          109 g          192 t
ORIGIN      
        1 gaggactaca tatgcgttca tcaaaatata ctgaacatta tacagatact tttataacat
       61 tcaaggaaat tatgagaact gtttctaaca tataccacaa ttgtgtacca gataagatta
      121 aaaaccgaag aaatactgat caactgaagc aacgcgatac agtgattatt gcttgtgtta
      181 tatggggtat aattaatggc tatactagcc aaagagccac gtatagagcg gtttgttccg
      241 tcttatttcc taatggtgac tttcctagta ggagtcgatt cactcgctta agttcaaact
      301 tagcttacac tatcaagatt attcgatact ttttcatcaa gaagctcacc aaaggtgaac
      361 tagtcggaat tatagatagt tttcctagtc cattatgtaa accagttaga aatagacaag
      421 caaaactatt gaatcaaata gccaaagttg gctataactc aacaaaaaaa tcttattttt
      481 atggtcttaa aattcatatg atcgttacta agactggctt tccgattact tactcaatca
      541 cgaatccagg tgtccatgac gtgaaagtgt tagagacttt atcggaagaa gcgaatcttc
      601 ccaatatcct aggggacaaa ggatatatta gccataaaa
//
LOCUS       DAHHZJ010000333          631 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.347, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000333 DAHHZJ010000000
VERSION     DAHHZJ010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.347"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..416
                     /locus_tag="NBL02_002947"
     CDS             <1..416
                     /locus_tag="NBL02_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCD2573550.1"
                     /translation="KVEYIYEFEGGKGITNQYRPNKFETFKEFYSLANTVTDDINKDG
                     MAALLLKGTTSVIFSIYSTSARTSKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITK
                     LLYNMPAPLFLSAVKVGSFVEVKMPGLMAALKRVY"
     gene            435..>631
                     /locus_tag="NBL02_002948"
     CDS             435..>631
                     /locus_tag="NBL02_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HCD2573551.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNP"
BASE COUNT          224 a           87 c          129 g          191 t
ORIGIN      
        1 caaaagtaga atatatttat gaatttgaag gtgggaaggg gatcactaat caataccgac
       61 ctaacaagtt cgaaacattt aaagaattct attcattagc aaatactgta acagacgata
      121 ttaataaaga cggtatggcg gcgcttcttt taaaagggac aacttcagtt attttttcta
      181 tttatagtac ctctgcaaga acttcaaaag agaagaaaga cgaagcaaag cagatattga
      241 gtgatccagt gatccgagaa gccaagcaaa ttgctaagac ggattcttca gcaaaaaaaa
      301 taacaaagtt gctttacaat atgccagctc cactattttt atctgctgta aaagttggaa
      361 gcttcgttga agtgaaaatg ccagggttaa tggctgcttt gaagcgagta tattagttaa
      421 tggaggaaaa tataatgaaa agaattttgc attttcaggg acgaatgggt ttaggtggag
      481 cagagtcttt catgatgaat ttatatcgta aaattgatcg tacaaactat caatttgatt
      541 ttttgattta tgaagattat gcagatgttc aagactatca ttcagagatt gaaagattag
      601 gaggccgtat ttttgttgta cctaatccta a
//
LOCUS       DAHHZJ010000334          618 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.348, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000334 DAHHZJ010000000
VERSION     DAHHZJ010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.348"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..>618
                     /locus_tag="NBL02_002949"
     CDS             <1..>618
                     /locus_tag="NBL02_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein"
                     /protein_id="HCD2573552.1"
                     /translation="SGKSLEALELEQEEIRRFQNCSLYPVYHGSAKSNIGIEQLIEVI
                     TNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILHLRDSVRISEKEKIKIT
                     EMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLPQRERIENPLPLLQTTV
                     EPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLGKVQM"
BASE COUNT          223 a          100 c          123 g          172 t
ORIGIN      
        1 tccggtaaat cattagaagc attagaactc gaacaagagg aaatcagaag atttcagaat
       61 tgctccttgt accctgttta tcatggaagc gcaaaaagca acatagggat tgagcagctt
      121 atagaagtga taacgaataa attttattca tcaacataca gaaagaagtc tgaactttgc
      181 ggaaatgtct tcaaaattga atattcggaa gaaagacaac gtcttgcata tgtacgcctt
      241 tatggcggaa tcctgcattt gcgggattcg gttagaatat cggaaaagga aaaaataaaa
      301 attacagaaa tgtatacttc aataaatggt gaattatgta aaattgataa ggcttattcc
      361 ggggaaattg ttattttgca aaatgagttt ttgaagctaa atagtgttct tggagataca
      421 aagctattgc cacagagaga gagaattgaa aatccgctcc ctctgctgca aacaactgtt
      481 gaaccgagca aacctcaaca aagggaaatg ttacttgatg cacttttaga aatctccgac
      541 agtgacccgc ttctacaata ttatgtggat tctacgacac atgaaattat actttctttc
      601 ttagggaaag tacaaatg
//
LOCUS       DAHHZJ010000335          617 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.349, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000335 DAHHZJ010000000
VERSION     DAHHZJ010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.349"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(<1..246)
                     /locus_tag="NBL02_002950"
     CDS             complement(<1..246)
                     /locus_tag="NBL02_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HCD2573553.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKL"
     gene            complement(251..436)
                     /locus_tag="NBL02_002951"
     CDS             complement(251..436)
                     /locus_tag="NBL02_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HCD2573554.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
BASE COUNT          187 a          125 c           72 g          233 t
ORIGIN      
        1 caacttatac caaggaaaaa aatactctcg ataaaaatca tgaaacgtta ttccttgcag
       61 ataaattcct tgtgaatcag aaaaactttg ttttaactct tcgagtcccc ttctagcttc
      121 tccttgagtt ttgaagcctc ttctggtcac ttgaattctt tttccaaagc gatcttgtcc
      181 taaagttgca gaaaaaaacc aagtgcctct ttttctgtct ttataaacat tcgcaatttt
      241 cctcatttta ctattcctcc caagaaatat ggagaacttc ttctaaaatt gaaataggaa
      301 ttctttttat ttttgaatta cgataaaaaa agtatccttt ctcaactaaa agttctttac
      361 attccttgaa gattttatta gcatagtact gagaaaaacc tagattaatc aaatcttttc
      421 gagtagctgt tttcattata gcaccttcct tacttttttt catttatttc tatcagctgc
      481 ttttgtcaaa agcacatagg aatttcacct ctccccaagt ttacggcgag ccttacggaa
      541 gtatcattat cccaacttta aatcatcgct ttttattgac tataaaaatt attttagaat
      601 atttatcgct ccttctt
//
LOCUS       DAHHZJ010000336          607 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.351, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000336 DAHHZJ010000000
VERSION     DAHHZJ010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.351"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(353..598)
                     /locus_tag="NBL02_002952"
     CDS             complement(353..598)
                     /locus_tag="NBL02_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit S"
                     /protein_id="HCD2573555.1"
                     /translation="MIHVFSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEG
                     YKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          194 a          119 c           89 g          205 t
ORIGIN      
        1 taaactattt tgggttacat ggtatctgaa acagctaaaa ccttgtcatt atagcaaaaa
       61 atcactttat atccgtttca tttcagatac atttcactta agcggattcc aaaaaaacaa
      121 aagaaaaagg aacccctagt tagcctatgc tttttattgg ttcctctcaa aacacggtca
      181 tataacaacg gggtggggta aatgaatgaa aaaggtggag gaaacattat tcaatagctg
      241 tcttaaatac ttcgttataa aatgtgaaca aataaaagac atctatccct attttctaca
      301 cttccacttg actaccatct ttgaatcgta caattttctt aactcttctc gtttacctca
      361 attcattctc aagtggaatc aactcatcat ctaacatttt acgccatgct cgcttcattt
      421 cttgtgggta tttaaatggc ttcgcttctg gatgtttggc tttatatcct tcgtaatcct
      481 tactgttgat aattgctttc atattaggaa ttggatcttc acctggtaga tattgcagat
      541 tagaagcatg taattctttc tcagaaacaa accactcatt ggaaaagaca tgaatcaaat
      601 catcact
//
LOCUS       DAHHZJ010000337          602 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.352, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000337 DAHHZJ010000000
VERSION     DAHHZJ010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.352"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(316..579)
                     /locus_tag="NBL02_002953"
     CDS             complement(316..579)
                     /locus_tag="NBL02_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uberolysin/carnocyclin family circular
                     bacteriocin"
                     /protein_id="HCD2573556.1"
                     /translation="MKKNLLLVLPILGFAGFFVGVPMLSANIGISSYAAKKVIDIINT
                     GSTVATIISIVAAVVGGGLITAGIVATAKSLIKKYGAKYAAAW"
BASE COUNT          226 a          103 c           78 g          195 t
ORIGIN      
        1 cttccttttt tgtatggatt ttttcattta cacaaaatat tttatactct cctgaatttt
       61 ttcatgttga aaacatcact aatcaaaggc aaattataat ttagaaatag aatgaaaaca
      121 ctaaatgaaa atagaacatt agaaaaaata gaatcaaaaa aacccaagac tagtgccgaa
      181 ataagggaaa caatcgaagc tacaaaaaat aatgatttcg ttgaacagga ccaatttatt
      241 tgtttccgat atttctcaaa aagaactttc tttgggaaag ttaaaggcga ctgtaaagtc
      301 gcctttagtc cttttttacc aagctgctgc atatttagct ccatattttt taattaatga
      361 tttggctgta gcaacaatac cagctgtaat taatccgcct ccaacaactg cagcaacaat
      421 agaaatgatt gtagcaaccg tgctaccagt attaattatg tcaataactt ttttagctgc
      481 ataagaagaa attccaatgt tagcacttag cattggtaca ccaacaaaga atcccgcaaa
      541 acctaaaatt ggtaaaacta ataataaatt ctttttcata ataattcctc tattttttta
      601 tt
//
LOCUS       DAHHZJ010000338          594 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.353, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000338 DAHHZJ010000000
VERSION     DAHHZJ010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.353"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..581
                     /locus_tag="NBL02_002954"
     CDS             <1..581
                     /locus_tag="NBL02_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCD2573557.1"
                     /translation="LAPTESAYVWKEILQDLKDRGLKEVLLVVTDGLSGINDSIHSVY
                     PNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLVYQASSKEEANNQIRFMIDKWKKQ
                     YPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEGFNKQLKKYTKRKEQFPNEESLER
                     FLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          220 a           88 c           97 g          189 t
ORIGIN      
        1 gtcttgctcc aactgaatct gcttatgttt ggaaagagat tcttcaagac ctaaaagacc
       61 gtggtttaaa agaggtttta ttagtcgtaa ctgatggttt aagtggcatt aacgatagta
      121 ttcatagtgt ttatccaaat gctcaatttc aacaatgttg tgtccatata tctagaaata
      181 tcgctcataa ggttcgtgtt agtgatcgac aagaaatctg tagcgatttc aaattagttt
      241 atcaagcaag ttcaaaagaa gaagcaaata atcaaataag gtttatgata gataaatgga
      301 aaaagcagta tccacgagtg gttaaattgc tcatgaatcc tgctatatta accttttata
      361 atttccctcc gtcaatcaga agaactattt attcaactaa tttgattgaa ggttttaaca
      421 aacaattaaa gaaatatacc aaaagaaaag agcaatttcc taatgaagaa tctctggaga
      481 gatttctagt atctcaattc aacaactata atcaaaagtt tctatgccga gttcataaag
      541 gttttaaaga aatacatgat acattagaat caatgtttta acttgatcaa caga
//
LOCUS       DAHHZJ010000339          591 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.354, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000339 DAHHZJ010000000
VERSION     DAHHZJ010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.354"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..581
                     /locus_tag="NBL02_002955"
     CDS             <1..581
                     /locus_tag="NBL02_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCD2573558.1"
                     /translation="EYNRLVDFTTSHNKRYSLLFQWLYLTGMRSGEAIALSKDDVHIT
                     NNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAIYNELLELNPNGQ
                     FLFQTTKGTPFQLTAINTYLRSHKADMKIDKKLSSHIFRHTHISKLAELGTPLYAIQD
                     RVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
BASE COUNT          231 a           95 c          101 g          164 t
ORIGIN      
        1 atgaatataa tagactagta gatttcacaa cttcacacaa taaaagatat tctctccttt
       61 tccagtggct atatttgact gggatgaggt ctggtgaagc aatcgcatta agtaaagatg
      121 atgtacacat taccaataat aatgcatcag tagttataaa tgggacgatg atgtatagag
      181 aacgttcaat agctgatatg aaaaaatctg attctacaaa aacagctgcc ggaatgcgag
      241 aaattgattt accaaaaaaa gcgatagcta tttacaatga gcttctagaa ttaaatccaa
      301 atggtcaatt tctatttcaa acaacgaaag gaactccttt ccaactaaca gcaattaaca
      361 cctatttgag aagtcataaa gctgacatga agattgataa aaaacttagt tcacatattt
      421 ttagacatac ccatatttca aaattagcgg aactaggaac tcctctgtat gccattcagg
      481 atcgcgttgg tcacgaaaat agtgatatca ctgaaaaaat ttatttgcat gtgacaaaag
      541 gagtaaaaga aaaattgaaa gaagatatag aaaaactgta acttgttgcc c
//
LOCUS       DAHHZJ010000340          585 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.356, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000340 DAHHZJ010000000
VERSION     DAHHZJ010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.356"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
BASE COUNT          200 a           73 c          136 g          176 t
ORIGIN      
        1 aaaaaattta taaaaggagg tttcttgata tgataagaaa caaaaagggt ttgttgaaag
       61 ttgctttact ggcagctttt ttctaagtaa ctttttgaca agctcaatgg caacacaagc
      121 ggaagaagta atacaagaac taactgttat gaataacagc tagataaaat gggctatgta
      181 tgttatccaa caatttagta atcactagaa gcctagcgaa agttcgcttg ataagttata
      241 gatgagtatt gatttgtagt gtttccaaat caaaagggta cagagccaca tgaaatatgt
      301 tgtcctctgc agcgtcagga tgggaatcca taccatggag attcggagat ctgtgaggga
      361 atacttacgt ggttaatatg tcagttgtgt tggacgtagt aatggtcata gtgggtagat
      421 tgactgaatt cttatatgag aaaatgaaac caacaagaag atagggttat ttatacagaa
      481 ttggggatct tttctggaaa agtaatcgga taatgcgaga ttcgtacggt cttttttaaa
      541 tttctctaaa aaaaacggca tcgaatgggt tgggtgattg ataag
//
LOCUS       DAHHZJ010000341          585 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.357, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000341 DAHHZJ010000000
VERSION     DAHHZJ010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.357"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(233..382)
                     /locus_tag="NBL02_002956"
     CDS             complement(233..382)
                     /locus_tag="NBL02_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573559.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
BASE COUNT          182 a           94 c           91 g          218 t
ORIGIN      
        1 attatacaga taataacgga atattatcat cgaaacagct aacaaaattg tcataaaaaa
       61 cgagatggtt gatattagaa cttgtgtttt tgtttaggtc tagagataaa gagagacaat
      121 ttccaaatat atgtaatgaa tacaaaaaga cgtttgtcgc ggattaccgt tacaaacgtc
      181 ttttattgat tttccgagat ctcatcagtc gccatttgga atggttcact ccttatctag
      241 attcgtttaa tgctcggact gcttgttcga ttgtaatacc aaacgcaacg ttattttcgt
      301 tttgtgcatc aatcgccata aaacggttag aagttgtatc aaaagctacg cggtattgca
      361 tatttgtttt tgtaaacgtc ataaaattca tctcctttgt aatgtaattt agttcttgaa
      421 agtttccttt aaactttcga acttatacca agtttagcat cgtacgtttt ttgatgcaat
      481 taaaaagcat ttatcatttt ccttatttat tttttcttaa atggtttagc tattttcttt
      541 taatccgata agtagcccaa tcatcaaggt tacaaatact attgc
//
LOCUS       DAHHZJ010000342          545 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.358, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000342 DAHHZJ010000000
VERSION     DAHHZJ010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.358"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            17..499
                     /locus_tag="NBL02_002957"
     CDS             17..499
                     /locus_tag="NBL02_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573560.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
BASE COUNT          145 a           85 c          109 g          206 t
ORIGIN      
        1 gtttggggga acgttaatga gaagaaaagc agtaatcgat cggctttatt tatttttttg
       61 gttttttcct atcgttttgc taccttttac atgggtaaat gctgttgtac aaggtgcgga
      121 gatattaggc ctgatccaag gaagcggatt tgtggtttct aggaattttc cagctttgat
      181 tgttttctat ttagtgataa gtgtaggtct cttattacca aaatctctga tcaatcacta
      241 caattggcag ctgctttact tttcttgctt attagtattt acatctatgt tttctttgct
      301 gcctcgcttg attatcggcc ctaatggttt gagcgagagt ggattgaagg atttagctta
      361 ttcctccatg tatttcttga cgatcaaacc atggttctat ttagtaattg cctcatttat
      421 tcttgcaaca tttttttact tctggacaga aatcaggata aaaagtgaac gatctgtatc
      481 tataaaaaat aactattgat ggtggatcat cagtaaaaaa acatatgagc tgcgtaaaat
      541 gcatg
//
LOCUS       DAHHZJ010000343          539 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.359, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000343 DAHHZJ010000000
VERSION     DAHHZJ010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.359"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            170..364
                     /locus_tag="NBL02_002958"
     CDS             170..364
                     /locus_tag="NBL02_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573561.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          227 a           67 c           87 g          158 t
ORIGIN      
        1 tttaagaaca atagcctttt attatatata ttattcaaat tagttatatc gagtgaaaaa
       61 aactttcaaa ttgattcttt ttatttatca taaagatata gatacataat cattttcact
      121 tacatatatt tgaatattaa ctaaaaacag caagaaagaa gaggatcaaa tgtattatgt
      181 agaagtaaaa acaaaaggcg tcaaaaacaa acaatatgtg aaaggtatca gtaacgaata
      241 tcctttactt ggttcatgga aagaagctgc tcctttttcc aaaccatgtg ccataaaaat
      301 aaagaacgag ctagaaaaag aactgacttg tggtaaagct gttgtagata tcatcgaaaa
      361 gtaaatccaa tgtatagaaa ggaagatcac acatgaaaag cgacgtacaa attgctaaga
      421 ttatagaatt agaagaagaa gacgcagtgt tgacaattga aaaaaaataa atgtttgtat
      481 accatatgat tatttcaata tgtatcctgg aattggagat ctcgtagagc tatatcaag
//
LOCUS       DAHHZJ010000344          536 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.361, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000344 DAHHZJ010000000
VERSION     DAHHZJ010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.361"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            51..>536
                     /locus_tag="NBL02_002959"
     CDS             51..>536
                     /locus_tag="NBL02_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HCD2573562.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YL"
BASE COUNT          194 a           84 c          108 g          150 t
ORIGIN      
        1 tactatcaaa taaggtagaa taatagaaaa agatagcagg aggaatgacg atgaatcatt
       61 ttaaaggaaa gcaatttcag caggatgtga ttattgtagc cgtgggctac tatcttcgtt
      121 ataaccttag ctatcgtgaa gttcaagaaa tcttatatga tcgtggcatt aacgtttctc
      181 atacgacgat ttatcgttgg gtgcaagaat atggcaaact actctatcaa atttggaaaa
      241 agaaaaataa aaaatccttt tattcatgga aaatggatga aacgtacatc aaaattaaag
      301 gaaaatggca ttatttgtat cgagccatcg atgcagatgg tttaaccttg gatatttggt
      361 tacgtaaaaa acgggacaca caagcagcct atgcttttct taagcggtta gtgaagcagt
      421 ttgatgaacc gaaggttgta gtcacagata aagccccctc tattacaagt gcctttaaga
      481 aactaaaaga atacggcttt tatcaaggga cagaacatcg taccattaaa tacctg
//
LOCUS       DAHHZJ010000345          517 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.362, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000345 DAHHZJ010000000
VERSION     DAHHZJ010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.362"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            100..>517
                     /locus_tag="NBL02_002960"
     CDS             100..>517
                     /locus_tag="NBL02_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HCD2573563.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELE"
BASE COUNT          200 a           59 c           95 g          163 t
ORIGIN      
        1 gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact
       61 tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt
      121 ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca
      181 agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta
      241 gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat
      301 ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat
      361 gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga
      421 agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt
      481 gaattttcta tcaaagagct aaactatgag cttgagt
//
LOCUS       DAHHZJ010000346          506 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.363, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000346 DAHHZJ010000000
VERSION     DAHHZJ010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.363"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
BASE COUNT          168 a           95 c           66 g          177 t
ORIGIN      
        1 aattcgcttg tggcgaaacc cgacctggag tttgtctctg tattttgaac cctaaaaatt
       61 aatcaaacag actgatattc tcataatcag cgcgaaataa gccattaagt acagttttcc
      121 tacctatttt tgataacccg ctggttagtt aatattcctt ttccgagaga gataaatgat
      181 tggttaaatc tatatacatc tcaaccttta aaaatgtttt cgaaatactt catcagtaca
      241 tgtcatacaa attaccagtg cattctcaaa ttctagttct ttaattgatt tcagttgagg
      301 agcagttact tttggcggaa atttccattt ttgtccaaac ctcttccccc gatgaaattt
      361 ctttgttgca attattgcaa tgaaaaattt ttgtttcaaa aatatacata attacttcct
      421 ccaataaaaa ctatctacct ctcaaattat ttttctctaa taagaaaaac agaaaaggta
      481 ataagataca acagtgatga ttccta
//
LOCUS       DAHHZJ010000347          505 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.364, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000347 DAHHZJ010000000
VERSION     DAHHZJ010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.364"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..454
                     /locus_tag="NBL02_002961"
     CDS             <1..454
                     /locus_tag="NBL02_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HCD2573564.1"
                     /translation="ETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQLEELYQA
                     YQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTDPLFTYL
                     KNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          181 a           97 c           76 g          151 t
ORIGIN      
        1 tgaaactact ttagaagacg gcactgtcta ttcattgaaa aaatatgcag gcaaaccgat
       61 aatcattgtt aacactgcaa caaaatgcgg tctagcacct caatttaaac aattagaaga
      121 actttaccaa gcttatcaga cacaaggtct ggttatctta ggctttcctt ccaatcaatt
      181 taaacaagag ctagattcag cgactgaagc agcagaagct tgtaaaacca cttatggcgt
      241 taccttccct atgcatcaat tgattgacgt aaatggagaa aatacagatc ctctttttac
      301 ttatcttaaa aatgaagctc ctggtactgt cactaaagca ataaaatgga attttactaa
      361 atttttgatt gatagaaatg gcaaagtaat cgaacgattt gcaccacaaa cttctccatt
      421 gaaaatgacc gctgcaattg aatccgttct ttaacttaat aaaaataaaa aaagatgaac
      481 tatttctagt tcatcttttt gcctt
//
LOCUS       DAHHZJ010000348          494 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.365, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000348 DAHHZJ010000000
VERSION     DAHHZJ010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.365"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            200..>494
                     /locus_tag="NBL02_002962"
     CDS             200..>494
                     /locus_tag="NBL02_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573565.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKF"
BASE COUNT          195 a           60 c           89 g          150 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa ttta
//
LOCUS       DAHHZJ010000349          480 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.367, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000349 DAHHZJ010000000
VERSION     DAHHZJ010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.367"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            76..442
                     /gene="ssrA"
                     /locus_tag="NBL02_002963"
     tmRNA           76..442
                     /gene="ssrA"
                     /locus_tag="NBL02_002963"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          138 a           96 c          101 g          145 t
ORIGIN      
        1 ttttttattg aatttatccc aatatatgct atactaattt tgtatgaaaa atacacgtaa
       61 cccttgttag ctaacgggga cgttacggat tcgacaggca cagtcgagct tgaattgcgt
      121 ttcgtaggtt acgtctacgt aaaaacgtta cagttaaata taactgctaa aaacgaaaac
      181 aactcttacg ctttagctgc ctaaaaacag ttagcgtaga tcctctcggc atcgcccatg
      241 tgctcgagta agggtcctaa ctttagtggg atacgtttca actttccgtc tgtaagttga
      301 aaaagagaac atcagactag cgatacagaa tgcctgtcac tcggcaagct gtaaagtgaa
      361 tccttaaatg agttgactat gaacgtagat ttttaagtgg cgatgtgttt ggacgcgggt
      421 tcgactcccg ccgtctccat tgtgagattt cagcagattt cgctagatgc ttaaatcctt
//
LOCUS       DAHHZJ010000350          473 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.368, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000350 DAHHZJ010000000
VERSION     DAHHZJ010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.368"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..>473
                     /locus_tag="NBL02_002964"
     CDS             <1..>473
                     /locus_tag="NBL02_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCD2573566.1"
                     /translation="YLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIW
                     LRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKY
                     LNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVST"
BASE COUNT          161 a           92 c           95 g          125 t
ORIGIN      
        1 attatctttg gaagaagaaa aatagacaat ccttctattc atggaaaatg gacgaaacct
       61 atatcaaaat taagggacgt tggcattatc tttatcgtgc aattgatgcg gacggcttaa
      121 ccttagatat ctggttacga aagaaacggg atacgcaagc agcctatgct ttcttaaaac
      181 gactccataa acagtttggt gagccgaaag caattgtgac cgataaagca ccttctcttg
      241 gctccgcctt tagaaagtta cagagtgtgg gtttatatac taagacagag caccgaactg
      301 tgaagtatct taacaattta atagaacaag accatcgacc tattaaacga cgaaataaat
      361 tttatcaaag tctccgtaca gcctcttcca cgattaaggg catggagacc attcgaggaa
      421 tatataaaaa gaaccgaaga aatggaacgc tcttcggctt ttcggtgtct act
//
LOCUS       DAHHZJ010000351          427 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.369, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000351 DAHHZJ010000000
VERSION     DAHHZJ010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.369"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..421
                     /locus_tag="NBL02_002965"
     CDS             <1..421
                     /locus_tag="NBL02_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCD2573567.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT          160 a           65 c           86 g          116 t
ORIGIN      
        1 atcactagat cgtttaggaa gagattatga agatataaaa aatacagttg cttttatgaa
       61 acagaaaaaa gtagcgttaa aaatattaga tgcacagttt ttagatttta atactggaaa
      121 tgaactcctg gataccgcta tgttcgatat gtttttatca ctgctaagtt atattgcaca
      181 aaacgagcgt gagaaaattc gtgaacgtca gcggcaagga gtattattgg ctcaaaaagc
      241 cggccgatat aagggacgac caatcgagta ttctattaac tcaacagatc cgcaaaaacg
      301 cttagttttt aaaaccgtgg tggacatgtt aaagcaaggc attccagttg cagagattgc
      361 aaaagaaaac gggatctctc gaccaactat ttataaaata aagaaagcga atgatttgta
      421 gaaatat
//
LOCUS       DAHHZJ010000352          426 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.370, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000352 DAHHZJ010000000
VERSION     DAHHZJ010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.370"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(192..>426)
                     /locus_tag="NBL02_002966"
     CDS             complement(192..>426)
                     /locus_tag="NBL02_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573568.1"
                     /translation="YKVWDSEVFFLAIEDSLLINLYYSNKNYFKIPATKTRMKKDVYF
                     LFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
BASE COUNT          147 a           71 c           61 g          147 t
ORIGIN      
        1 aaaaataatg ggaaaaagac ttataattgg agtggtagtt aattagtgac ttatttttga
       61 ttttatagca ctgatactat aaaatataga tatcatcata ttacacaatc ttaatactaa
      121 cttaaaaaat atccctttta taagtatggt gataaaatcc gttccgggct acctttttta
      181 ggtagcctac tttaatcttt atacctttct ggatcaacga aagtatactt caaatagtca
      241 taacgccgat gatcgctccg tgcgtctggc acatcagtca cgatatcaaa caaaaaatac
      301 acatctttct tcattctggt ttttgttgcc ggaattttaa agtagttctt attagagtaa
      361 tagagattga ttaataagct atcttcgatt gctaaaaaga aaacttctga atcccacacc
      421 ttataa
//
LOCUS       DAHHZJ010000353          425 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.371, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000353 DAHHZJ010000000
VERSION     DAHHZJ010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.371"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(87..>425)
                     /locus_tag="NBL02_002967"
     CDS             complement(87..>425)
                     /locus_tag="NBL02_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HCD2573569.1"
                     /translation="LERMAGRSLIDIKSPIITDMPKAPKHGNKAEDAIIQMMDIEAER
                     DAILAALMALSLISRQILYYSFCVPDSFSNYRISREVGYSERSIQRMKSEALIEFAEA
                     YKHGRIIAYK"
BASE COUNT          118 a          113 c           67 g          127 t
ORIGIN      
        1 agacctatca ctaatctttc gacactacca taatatcact ggtaaatagc taaaaaccgc
       61 catcattccg ccaaaaagcc gccaaattat ttataagcaa ttattcttcc gtgtttatat
      121 gcttctgcaa actctattag agcttccgac ttcatccgtt gtatacttct ttctgaataa
      181 cccacttcac ggctaattct gtagtttgag aagctatctg gcacacaaaa gctatagtag
      241 agtatctgac gactaatcag actaagagcc atcaaggctg ctagaatcgc gtctctctcc
      301 gcttctatat ccatcatctg aatgattgcg tcttctgcct tattgccgtg cttcggcgcc
      361 ttcggcatat ccgtaataat cggcgactta atatctatca aagagcgacc tgccatccgc
      421 tccaa
//
LOCUS       DAHHZJ010000354          424 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.373, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000354 DAHHZJ010000000
VERSION     DAHHZJ010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.373"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(170..415)
                     /locus_tag="NBL02_002968"
     CDS             complement(170..415)
                     /locus_tag="NBL02_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit S"
                     /protein_id="HCD2573570.1"
                     /translation="MIHAFSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEG
                     YKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          130 a           83 c           58 g          153 t
ORIGIN      
        1 tgcaacagaa ccggtaaatg aatgaaaaag atggaagaat cattattcaa tagctttctt
       61 aaatactccg ttataaaatg tgaacaagta aaaagacatc tatccctatt ttctacactt
      121 ccactttact actatctttg aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt
      181 cattttcaag tggaatcaac tcatcatcta acattttacg ccatgctcgc ttcatttctt
      241 gtgggtattt aaatggtttc gcttctggat gtttggcttt atatccttcg taatccttac
      301 tgttgataat tgctttcata ttaggaattg gatcttcacc tggcatatat tgcagattag
      361 aagcatgtaa ttctttctca gaaacaaacc actcattgga aaaggcatga atcaaattat
      421 cact
//
LOCUS       DAHHZJ010000355          421 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.374, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000355 DAHHZJ010000000
VERSION     DAHHZJ010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.374"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
BASE COUNT          158 a           51 c           86 g          126 t
ORIGIN      
        1 tcattggtcg ttgaatgggc tgaatagtag tgtataaaac aattttgtgg agcgtgaagc
       61 ttttatggta gaggaaaata cgaataaagt gatgttgata aaaagattcc tatcttgaat
      121 atgaatcgag atgtcttgaa aatgaatgag tttgaccaat cattatccta tcgaaattta
      181 cagtttcgtt cgattggtct gtcaattaat gaaaaagtaa aaaagaatat gttctaggaa
      241 gagggcggca catctaaatt tttaagtaac ggacagttca aatatcaagc attgaaagag
      301 gctatcctac tgtttgaagg aatacgaata tataaaaacg acaaagagga aaaagtagtt
      361 cattaaatcc cttcaagtga tgaaagaatg agatctaata aaacctgatc tttcatctgt
      421 t
//
LOCUS       DAHHZJ010000356          415 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.376, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000356 DAHHZJ010000000
VERSION     DAHHZJ010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.376"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(<1..339)
                     /locus_tag="NBL02_002969"
     CDS             complement(<1..339)
                     /locus_tag="NBL02_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD2573571.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DAHHZJ010000357          411 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.377, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000357 DAHHZJ010000000
VERSION     DAHHZJ010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.377"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..>411
                     /locus_tag="NBL02_002970"
     rRNA            <1..>411
                     /locus_tag="NBL02_002970"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          108 a           91 c          131 g           81 t
ORIGIN      
        1 gaaagccccc ggctcaaccg gggagggtca ttggaaactg ggagacttga gtgcagaaga
       61 ggagagtgga attccatgtg tagcggtgaa atgcgtagat atatggagga acaccagtgg
      121 cgaaggcggc tctctggtct gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag
      181 gattagatac cctggtagtc cacgccgtaa acgatgagtg ctaagtgttg gagggtttcc
      241 gcccttcagt gctgcagcta acgcattaag cactccgcct ggggagtacg accgcaaggt
      301 tgaaactcaa aggaattgac gggggcccgc acaagcggtg gagcatgtgg tttaattcga
      361 agcaacgcga agaaccttac caggtcttga catcctttga ccactctaga g
//
LOCUS       DAHHZJ010000358          399 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.378, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000358 DAHHZJ010000000
VERSION     DAHHZJ010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.378"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
BASE COUNT          111 a           98 c           76 g          114 t
ORIGIN      
        1 tataaataac tcataaaaaa tattttgact ttcaaaatca cttttgctac cagaagttga
       61 ggcgaagtgt tttaagcata gttgtaccct agtttgaccg ttaaaaataa ataacgatcg
      121 ctagagtgca actatgtttg tttgccctac cgtcagcgtc ggcttggcct ctcacatcca
      181 ttaccgtcgg gattatctcc gttacatacc cttacaaaat acgccctgta agcattgcat
      241 gaaaccaccg tgcatttgca ccgtatttcc gccctagccg ttcaaatcgg tcgctaccac
      301 cattcagaaa cacgtgcggt aatggaaggt ttggaggaag ctatccagac cactaacgat
      361 tgtccctatc gtcgtacgtt tcactcagat caaggttgg
//
LOCUS       DAHHZJ010000359          362 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.382, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000359 DAHHZJ010000000
VERSION     DAHHZJ010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.382"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(<1..>362)
                     /locus_tag="NBL02_002971"
     rRNA            complement(<1..>362)
                     /locus_tag="NBL02_002971"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           72 a          113 c           83 g           94 t
ORIGIN      
        1 aaatccggac aacgcttgcc acctacgtat taccgcggct gctggcacgt agttagccgt
       61 ggctttctgg ttagataccg tcaagggatg aacagttact ctcatccttg ttcttctcta
      121 acaacagagt tttacgatcc gaaaaccttc ttcactcacg cggcgttgct cggtcagact
      181 ttcgtccatt gccgaagatt ccctactgct gcctcccgta ggagtttggg ccgtgtctca
      241 gtcccaatgt ggccgatcac cctctcaggt cggctatgca tcgtggcctt ggtgagccgt
      301 tacctcacca actagctaat gcaccgcggg tccatccatc agcgacaccc gaaagcgcct
      361 tt
//
LOCUS       DAHHZJ010000360          354 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.383, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000360 DAHHZJ010000000
VERSION     DAHHZJ010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.383"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            2..184
                     /locus_tag="NBL02_002972"
     CDS             2..184
                     /locus_tag="NBL02_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HCD2573572.1"
                     /translation="MKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLGLVPVESVEE
                     VLGMKIEVEEDFQNHG"
     gene            177..326
                     /locus_tag="NBL02_002973"
     CDS             177..326
                     /locus_tag="NBL02_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCD2573573.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
BASE COUNT          149 a           37 c           79 g           89 t
ORIGIN      
        1 aatgaaatta ggttatggga agtatcagtc tgagagaatc atacgacagg caaaagcaat
       61 tatggtttca aaaagctata cctattacga aaatagaaga ttaggactag tacctgttga
      121 atctgtagaa gaagttttag gtatgaagat agaggtagag gaggattttc agaatcatgg
      181 ttaattttaa aaaagataaa aatgggaaat atcattttac tgctagtttg ggattcgaca
      241 aacgaacagg gaaaagagta caaaaaaacg tagaggggtt aaaacagtta aagaagaaaa
      301 aaagcgtata ccgaacttgt ttctaattat ggaaaagaag cagaaacttc tcaa
//
LOCUS       DAHHZJ010000361          344 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.385, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000361 DAHHZJ010000000
VERSION     DAHHZJ010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.385"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="NBL02_002974"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="NBL02_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HCD2573574.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT          107 a           51 c           56 g          130 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttgtatagac tgattgtaac atgg
//
LOCUS       DAHHZJ010000362          340 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.386, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000362 DAHHZJ010000000
VERSION     DAHHZJ010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.386"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            67..264
                     /locus_tag="NBL02_002975"
     CDS             67..264
                     /locus_tag="NBL02_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HCD2573575.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            266..>340
                     /locus_tag="NBL02_002976"
     CDS             266..>340
                     /locus_tag="NBL02_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HCD2573576.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYS"
BASE COUNT          128 a           34 c           67 g          111 t
ORIGIN      
        1 gttttttgaa atttaatgaa tgttagaata atagtctaaa aacattaatt taggggtgat
       61 tagattatga aacatttaaa aattttgtct attaaagaga cacaacttat ctatgggggt
      121 accactcata gtggaaaata ttatggaaat ggagtgtatt gcactaaaaa taaatgtacg
      181 gtcgattggg ccaaggcaac tacttgtatt gcaggaatgt ctataggtgg ttttttaggt
      241 ggagcaattc cagggaagtg ctaaaatgaa aaaaaatgct aagcaaattg ttcatgaatt
      301 atataatgat atatctataa gtaaagatcc taaatattct
//
LOCUS       DAHHZJ010000363          327 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.387, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000363 DAHHZJ010000000
VERSION     DAHHZJ010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.387"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(48..>327)
                     /locus_tag="NBL02_002977"
     CDS             complement(48..>327)
                     /locus_tag="NBL02_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573577.1"
                     /translation="SRAYYSAFKKSDEYLKENYDIIYNGSSGKGSHQTVWNLFSTVKE
                     LNTLGIHNSGYRMLEKRKCADYIPNETITKTDMALMNREAEKIINKLT"
BASE COUNT          101 a           60 c           38 g          128 t
ORIGIN      
        1 tacaaagatt aaataaaaaa ataacaaaca aactcgcttg aaattcatta ggtgagtttg
       61 tttattattt tttctgcttc tcgattcatc agcgccatat ctgttttcgt aatggtttca
      121 ttaggaatat agtcagcaca ttttcttttc tctaacattc tatacccact attatgtatt
      181 cctaaggtat tcaattcctt aactgttgaa aacaaattcc aaacggtttg gtggcttcct
      241 tttccgctac ttccattata aataatatca taattttctt tcaaatactc atcagatttt
      301 ttaaaagcac tgtagtatgc tcttgaa
//
LOCUS       DAHHZJ010000364          316 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.389, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000364 DAHHZJ010000000
VERSION     DAHHZJ010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.389"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(83..244)
                     /locus_tag="NBL02_002978"
     CDS             complement(83..244)
                     /locus_tag="NBL02_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCD2573578.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
BASE COUNT          101 a           64 c           33 g          118 t
ORIGIN      
        1 tgataaaaat tattaaagtt ctatttttct ttcttgtaat atatgctata gcatttttta
       61 tcactttcct aactactcct ctctattttc ttactccata actaattgca tccactggac
      121 aagcattttt acatcgtcca catctgatac actccagatg attgcaattc tcaactgaat
      181 ctttattcat ttggcaaact tttttacatt ttccacaatt aatacactta tccttatcct
      241 gaatcggcta cactagacta gacagaaaaa ataaggtgtg tagaataata aaaaacacac
      301 tggaggattt ttcatg
//
LOCUS       DAHHZJ010000365          308 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.390, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000365 DAHHZJ010000000
VERSION     DAHHZJ010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.390"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..>308
                     /locus_tag="NBL02_002979"
     CDS             <1..>308
                     /locus_tag="NBL02_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCD2573579.1"
                     /translation="RNGKFSQQTLPAYKRTNDSLETTIIQLFQKGITMSEISELIEKM
                     YGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVIFMDATHIPLKRQTVSKEAVYIV
                     "
BASE COUNT          115 a           53 c           41 g           99 t
ORIGIN      
        1 atagaaatgg taagttttct caacaaactt tacctgctta taaaagaacc aatgactctt
       61 tagaaaccac tattattcag ctatttcaaa aaggaattac tatgtctgaa atctctgaat
      121 tgattgaaaa aatgtacggg catcactata cgccacaaac catttcaaat atgactaaaa
      181 tagtttctga agatattatt gcctttaaag aaaggtcctt agaatccaga tactctgtca
      241 tttttatgga tgctactcac attcctttaa aaagacaaac tgtttcaaaa gaagcagttt
      301 atattgta
//
LOCUS       DAHHZJ010000366          300 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.393, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000366 DAHHZJ010000000
VERSION     DAHHZJ010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.393"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..>300
                     /locus_tag="NBL02_002980"
     rRNA            <1..>300
                     /locus_tag="NBL02_002980"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           95 a           66 c           85 g           54 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
//
LOCUS       DAHHZJ010000367          297 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.394, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000367 DAHHZJ010000000
VERSION     DAHHZJ010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.394"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            10..210
                     /locus_tag="NBL02_002981"
     CDS             10..210
                     /locus_tag="NBL02_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCD2573580.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT          103 a           45 c           64 g           85 t
ORIGIN      
        1 aatttattta tgaataacgg tacagtaaaa tggttcaacg cagacaaagg ttttggcttt
       61 atcactggtg aagatggaaa tgatgtattt gcacattttt cagctatcca aggtgacggc
      121 ttcaaaactt tagaagaagg ccaagcagtg tcttacgatg ttgaagatgg tcaacgtggc
      181 cctcaagcaa caaacattgt taaagcttaa taaaaattga cttttaaccg tcttttcgaa
      241 agaaaagggc tctttgtcaa ataggactga tagagtgaga taagaacatc taaggta
//
LOCUS       DAHHZJ010000368          288 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.396, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000368 DAHHZJ010000000
VERSION     DAHHZJ010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.396"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            103..>288
                     /locus_tag="NBL02_002982"
     CDS             103..>288
                     /locus_tag="NBL02_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD2573581.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKI"
BASE COUNT          117 a           31 c           48 g           92 t
ORIGIN      
        1 atattaataa gtattattgt tttatcagtt gtatttgctt cttcattaat gttaatcaag
       61 aagcctaaag aaatcttatc aaaaataagt taggagaaaa taatggatat attagaaata
      121 aagaatgtag catatagtta tgcaaattct aaagaaaaag ttttgtcagg agtaaatcaa
      181 aaatttgaac ttgggaagtt ttatgcgata gtaggaaagt caggaacagg aaaatctaca
      241 cttctttcct tacttgctgg acttgataaa cctcaaacag gaaaaata
//
LOCUS       DAHHZJ010000369          287 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.397, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000369 DAHHZJ010000000
VERSION     DAHHZJ010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.397"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
BASE COUNT           99 a           41 c           49 g           98 t
ORIGIN      
        1 caatatatta agtctaaagt gacgcataat aaaaaaattt cttatcaata ccatgtagga
       61 cagtatttac ccgactggca tccttgggaa gattatcgca atttttttgt ttcaaacaaa
      121 aaaactaatg ggtgtagaga aatattcgct attgaattac cttggattat agatacattt
      181 gggaaagtta aatcgtttac tgtaatgacc cagaaattat ccaatcttga tattgattat
      241 tcggacagtt tcactcttat tattgagcat gaaactggta tagttgg
//
LOCUS       DAHHZJ010000370          283 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.398, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000370 DAHHZJ010000000
VERSION     DAHHZJ010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.398"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            1..283
                     /locus_tag="NBL02_002983"
                     /pseudo
     CDS             1..283
                     /locus_tag="NBL02_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          109 a           57 c           56 g           61 t
ORIGIN      
        1 aacaaatcgt cgatctctat ctcgctggta agcctcgcgc agaaattatt cgagagtatg
       61 agcttacgcc ttcttctttc gataaatgga tgaagcaagc acaatcaacg ggctcattca
      121 aagaaagaga caacttaaca ccagaacaag cagaattgat cgcactaaga aagaaaaata
      181 agcaactcga aatggagaat gatattttaa agcaagcggc gctgatattc ggacgaaaag
      241 acaagtaatt gatgccaaca agcataaata ttccatatca gcg
//
LOCUS       DAHHZJ010000371          276 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.400, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000371 DAHHZJ010000000
VERSION     DAHHZJ010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.400"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            71..>276
                     /locus_tag="NBL02_002984"
     CDS             71..>276
                     /locus_tag="NBL02_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCD2573582.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRT"
BASE COUNT           99 a           40 c           46 g           91 t
ORIGIN      
        1 tagaaaaaaa gaaagtccat tctgtaaaat taaagttacc acaaccgaaa ttttatagga
       61 ggactttctc atgcccaatt ttactacaga aattatggaa acactaatca ataaaggtga
      121 tttagatgaa ttatttcgat gtcatttaga attagctgtt aattcattat tgcaggctga
      181 attaacagct tttcttgatt atgaaaaata tgatcgtgct gggttcaatt caggtaattc
      241 ccgtaatggg aattattcac gttcattcag aacaga
//
LOCUS       DAHHZJ010000372          269 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.401, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000372 DAHHZJ010000000
VERSION     DAHHZJ010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.401"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
BASE COUNT           99 a           37 c           43 g           90 t
ORIGIN      
        1 gtttggattt gttatgaata ttctgaaata ctcagaatat gaaagtgctc aaataggaaa
       61 tgacaatggt tcattaagat ctactatatt accatttgct cagtctgatg tggtaaatac
      121 tgtagttcaa ggggtagatc cacaaattac taattatctg tcgagtcaag tcgatagttt
      181 tgacgataac ggaaaaaata cctatattaa tattataaag aatatttcag agtttcaaca
      241 aaatcaattc atatttccgt tactaaaca
//
LOCUS       DAHHZJ010000373          256 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.403, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000373 DAHHZJ010000000
VERSION     DAHHZJ010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.403"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
BASE COUNT           93 a           32 c           42 g           89 t
ORIGIN      
        1 atgtatcaag aaagttaggg gcagaaaact taggtataaa tacatatact caatccctag
       61 tattgctttt aggattattt atcaattcta gcattctaca aattggcgca agagaaatcg
      121 ctagacagga tagaattagt agaacaaaaa catttattac actttggtta ttacaagtac
      181 tatgtgggtt ggttattatc atagtgtata tattaatcat tatgggtagc aaatactatt
      241 attattattt actaca
//
LOCUS       DAHHZJ010000374          255 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.404, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000374 DAHHZJ010000000
VERSION     DAHHZJ010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.404"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..86
                     /locus_tag="NBL02_002985"
     CDS             <1..86
                     /locus_tag="NBL02_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HCD2573583.1"
                     /translation="VHYATDNLELFLPLGYSRKDLEVQLHD"
     gene            79..243
                     /locus_tag="NBL02_002986"
     CDS             79..243
                     /locus_tag="NBL02_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573584.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
BASE COUNT          109 a           29 c           52 g           65 t
ORIGIN      
        1 aagttcatta tgctacggat aatctagaat tatttttacc actagggtat agtaggaaag
       61 atttggaggt tcaattacat gactgagaaa gaacaagtaa cgaaaattgt aaagaaatac
      121 aataagagca ttgcggattt atcggaaaat gctactgcaa aagagttcaa aacggtgatt
      181 aaatatgtag ccgatcaggc caatgaaaaa caacggaaat tagttggatt aaataaaaaa
      241 tagtaggaca aatta
//
LOCUS       DAHHZJ010000375          245 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.407, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000375 DAHHZJ010000000
VERSION     DAHHZJ010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.407"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..>245
                     /locus_tag="NBL02_002987"
     CDS             <1..>245
                     /locus_tag="NBL02_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCD2573585.1"
                     /translation="SKAIIALKPEGRKAVDIENSINEWLQSVPKNLFKSITFDCGKEF
                     SNWKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRK"
BASE COUNT           88 a           47 c           43 g           67 t
ORIGIN      
        1 tcaaaagcca ttatcgcctt gaaaccagaa ggccgtaagg cagttgatat tgaaaattcg
       61 attaatgaat ggcttcaatc cgtacccaaa aatcttttca aatcaattac ctttgattgt
      121 ggaaaagaat tctctaattg gaaaagtatt agtaatacga atgatattga tatttatttc
      181 gcagacccag gaacgccttc ccaacgggga ttaaatgaac attcaaacgg actcctccga
      241 aaaga
//
LOCUS       DAHHZJ010000376          242 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.408, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000376 DAHHZJ010000000
VERSION     DAHHZJ010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.408"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..195
                     /locus_tag="NBL02_002988"
     CDS             <1..195
                     /locus_tag="NBL02_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HCD2573586.1"
                     /translation="TILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSY
                     EQCALMERAIAAKRKELDKK"
BASE COUNT           91 a           33 c           54 g           64 t
ORIGIN      
        1 acgattctag aagctttcaa tgagcagatg ggtaaagata tgaccaaaac cttaatcgaa
       61 tatgttaatg agcagacaga taaattaggg ctcttagctg ataacgttga aactattgaa
      121 cagttaagct atgagcaatg tgccttgatg gagcgagcaa tagcagctaa gagaaaagaa
      181 ttagataaga agtgatatga gtgtttaaac cattaatcga ttcatattca gcggttctga
      241 aa
//
LOCUS       DAHHZJ010000377          237 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.410, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000377 DAHHZJ010000000
VERSION     DAHHZJ010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.410"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(<1..216)
                     /locus_tag="NBL02_002989"
     CDS             complement(<1..216)
                     /locus_tag="NBL02_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCD2573587.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIF"
BASE COUNT           62 a           44 c           44 g           87 t
ORIGIN      
        1 aaaaatgatt tcggttctcc cacaacgtcg cttattttct ttgtattgtt caaagtattc
       61 aagtgctgtt ccaccacatt tgagaaagtt atagacattg taaattgttt ggcgtccacg
      121 tttaagctgc ttcgcaacga tagcaaccgg agtttcttga tgaaaatatg cttctatcat
      181 tacaagctcg tttggtgtaa gatgggtata agtcatttat gttcactctc cttgtat
//
LOCUS       DAHHZJ010000378          233 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.411, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000378 DAHHZJ010000000
VERSION     DAHHZJ010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.411"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
BASE COUNT           79 a           35 c           45 g           74 t
ORIGIN      
        1 tgagaaaaag catggaatgc gctgcatcag gtatcgaggc ttagaaaaaa atcaggtgca
       61 tacgatgctt gtttttgccg aaatgagtct gaaaaaattg gtcacttagc agtgggagca
      121 tatgtcttca tattttttat tatacaaaaa tagacaaaag tatcagtcta cactcttagt
      181 aaagaggaaa ctgatacttt tgtctacagt atgatatttc ttacatctac ttt
//
LOCUS       DAHHZJ010000379          224 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.413, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000379 DAHHZJ010000000
VERSION     DAHHZJ010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.413"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..>224
                     /locus_tag="NBL02_002990"
     CDS             <1..>224
                     /locus_tag="NBL02_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCD2573588.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAA
                     ESQTPAKPAAEPTVDTESAGSSSEGVFQFK"
BASE COUNT           70 a           47 c           58 g           49 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agtt
//
LOCUS       DAHHZJ010000380          222 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.414, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000380 DAHHZJ010000000
VERSION     DAHHZJ010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.414"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..>222
                     /locus_tag="NBL02_002991"
     CDS             <1..>222
                     /locus_tag="NBL02_002991"
                     /inference="COORDINATES: protein motif:HMM:NF025072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCD2573589.1"
                     /translation="SKIKMFELYRDNDVLVFPSLADGFGLAVIEALSNGMYVICSDST
                     GAKDIIMEGKNGDVFLTGDENDLNNKIQY"
BASE COUNT           79 a           18 c           45 g           80 t
ORIGIN      
        1 tatcaaaaat aaaaatgttt gaactgtaca gggataatga tgttttagta tttccttctt
       61 tagcggatgg ttttggttta gctgttattg aagcattaag caatggaatg tatgttatat
      121 gttcagattc tacaggtgct aaagatatta ttatggaagg caaaaatgga gatgtgtttc
      181 ttacaggaga tgaaaatgat ttaaataata aaatacaata tt
//
LOCUS       DAHHZJ010000381          221 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.415, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000381 DAHHZJ010000000
VERSION     DAHHZJ010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.415"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..>221
                     /locus_tag="NBL02_002992"
     CDS             <1..>221
                     /locus_tag="NBL02_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD2573590.1"
                     /translation="LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVS
                     DAPIILADEPTGNLDSVTAGEIINILKTL"
BASE COUNT           89 a           26 c           43 g           63 t
ORIGIN      
        1 gattagtaaa taaatcagca gatgaaagta tcttgtttga actaggttta gataaaaaac
       61 aaataaaaag aaatgttatg aaattatctg gtggacagca acaaagagta gctattgcta
      121 gagcattggt atcagatgct ccaataatac tagctgatga gcctactggt aatctagaca
      181 gtgttactgc tggagaaata attaatatat taaagacatt a
//
LOCUS       DAHHZJ010000382          215 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.denovo.420, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000382 DAHHZJ010000000
VERSION     DAHHZJ010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.denovo.420"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            complement(<1..>215)
                     /locus_tag="NBL02_002993"
     CDS             complement(<1..>215)
                     /locus_tag="NBL02_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCD2573591.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTS"
BASE COUNT           66 a           40 c           42 g           67 t
ORIGIN      
        1 ggaagttgtg aatcgttgaa tacgcaagaa atcagcgatt cttaatgcat cgtttcgatc
       61 agttttattc tcatcaaaca tgcgactaaa ttgtttcacc cgaaaaggat tttcaatcgt
      121 tacaagtgtg ttgagcttct ttagtttctc atcttcatga aaaaacatgg aaggatggaa
      181 gctgtacatg gacgttgact ccatgccaat cacaa
//
LOCUS       DAHHZJ010000383        29831 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.guided.017, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000383 DAHHZJ010000000
VERSION     DAHHZJ010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.guided.017"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            26..889
                     /locus_tag="NBL02_002994"
     CDS             26..889
                     /locus_tag="NBL02_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573592.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            926..1462
                     /locus_tag="NBL02_002995"
     CDS             926..1462
                     /locus_tag="NBL02_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HCD2573593.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            1476..1898
                     /locus_tag="NBL02_002996"
     CDS             1476..1898
                     /locus_tag="NBL02_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HCD2573594.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            1828..4227
                     /locus_tag="NBL02_002997"
     CDS             1828..4227
                     /locus_tag="NBL02_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HCD2573595.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            4259..6250
                     /locus_tag="NBL02_002998"
     CDS             4259..6250
                     /locus_tag="NBL02_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCD2573596.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            6273..6524
                     /locus_tag="NBL02_002999"
     CDS             6273..6524
                     /locus_tag="NBL02_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HCD2573597.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            6514..7743
                     /locus_tag="NBL02_003000"
     CDS             6514..7743
                     /locus_tag="NBL02_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HCD2573598.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            7740..9821
                     /locus_tag="NBL02_003001"
     CDS             7740..9821
                     /locus_tag="NBL02_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HCD2573599.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            9970..13890
                     /locus_tag="NBL02_003002"
     CDS             9970..13890
                     /locus_tag="NBL02_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HCD2573600.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            13891..14835
                     /locus_tag="NBL02_003003"
     CDS             13891..14835
                     /locus_tag="NBL02_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573601.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            14839..15042
                     /locus_tag="NBL02_003004"
     CDS             14839..15042
                     /locus_tag="NBL02_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HCD2573602.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(15342..15578)
                     /locus_tag="NBL02_003005"
     CDS             complement(15342..15578)
                     /locus_tag="NBL02_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD2573603.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(15761..16147)
                     /locus_tag="NBL02_003006"
     CDS             complement(15761..16147)
                     /locus_tag="NBL02_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HCD2573604.1"
                     /translation="MDARAMSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECI
                     CVQSISYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAA
                     CSKRMRRRKEAERQRKRYWKPTHLEL"
     gene            complement(16129..17457)
                     /locus_tag="NBL02_003007"
     CDS             complement(16129..17457)
                     /locus_tag="NBL02_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HCD2573605.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(17418..17747)
                     /gene="mobC"
                     /locus_tag="NBL02_003008"
     CDS             complement(17418..17747)
                     /gene="mobC"
                     /locus_tag="NBL02_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HCD2573606.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(18005..18376)
                     /locus_tag="NBL02_003009"
     CDS             complement(18005..18376)
                     /locus_tag="NBL02_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD2573607.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            18521..18706
                     /locus_tag="NBL02_003010"
     CDS             18521..18706
                     /locus_tag="NBL02_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCD2573608.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            18864..19031
                     /locus_tag="NBL02_003011"
     CDS             18864..19031
                     /locus_tag="NBL02_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573609.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            19649..20308
                     /gene="vanR"
                     /locus_tag="NBL02_003012"
     CDS             19649..20308
                     /gene="vanR"
                     /locus_tag="NBL02_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HCD2573610.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            20311..21654
                     /gene="vanS"
                     /locus_tag="NBL02_003013"
     CDS             20311..21654
                     /gene="vanS"
                     /locus_tag="NBL02_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HCD2573611.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RSDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            21825..22634
                     /gene="vanY"
                     /locus_tag="NBL02_003014"
     CDS             21825..22634
                     /gene="vanY"
                     /locus_tag="NBL02_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HCD2573612.1"
                     /translation="MEKKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGW
                     RLASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIP
                     AQYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKI
                     AEYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSY
                     RFGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            22652..23479
                     /gene="vanW"
                     /locus_tag="NBL02_003015"
     CDS             22652..23479
                     /gene="vanW"
                     /locus_tag="NBL02_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HCD2573613.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYPIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            23476..24447
                     /locus_tag="NBL02_003016"
     CDS             23476..24447
                     /locus_tag="NBL02_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HCD2573614.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            24440..25468
                     /gene="vanB"
                     /locus_tag="NBL02_003017"
     CDS             24440..25468
                     /gene="vanB"
                     /locus_tag="NBL02_003017"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HCD2573615.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            25474..26082
                     /locus_tag="NBL02_003018"
     CDS             25474..26082
                     /locus_tag="NBL02_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HCD2573616.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDKLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(26148..26321)
                     /locus_tag="NBL02_003019"
     CDS             complement(26148..26321)
                     /locus_tag="NBL02_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2573617.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
     gene            26670..27101
                     /locus_tag="NBL02_003020"
     CDS             26670..27101
                     /locus_tag="NBL02_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCD2573618.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
     gene            27108..27341
                     /locus_tag="NBL02_003021"
     CDS             27108..27341
                     /locus_tag="NBL02_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD2573619.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            27765..27965
                     /locus_tag="NBL02_003022"
     CDS             27765..27965
                     /locus_tag="NBL02_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCD2573620.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            28049..29242
                     /locus_tag="NBL02_003023"
     CDS             28049..29242
                     /locus_tag="NBL02_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCD2573621.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            29546..29794
                     /locus_tag="NBL02_003024"
                     /pseudo
     CDS             29546..29794
                     /locus_tag="NBL02_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
BASE COUNT         7581 a         7578 c         8103 g         6569 t
ORIGIN      
        1 gggcgcggga aaggaggtgc tacccgtgat tagtgaaatc attgaagaat ggataaaagg
       61 catcctcatg gacggtatca cgggcaacct gtccgggctg ttcgatacgg taaatgccaa
      121 agtcggagaa atcgccgccg acgtgggacg aaccccgcag gcatggaaca gcggtatttt
      181 caatatgctg cacagcctgt cggaaaccgt gatcgtcccc atagccgggg ccatactcgc
      241 ccttgtcatg tgctatgagc ttatccagat gataacggaa aagaacaata tgcacgactt
      301 tgacagctcc atgttcttcc gctggatatt caaatccgcg tttgcaatcc tgatcgtaac
      361 gaacacatgg aacatcgtca tgggcgtatt cgacgccacc caaagcgtcg tcaatcaaag
      421 cgccggtgtg attatcgggc agacatccat tgattttaac accctgatac cagaccttga
      481 aagccaattg gaggctatga gcgtgggcgc tcttttgggg ctgtggttcc aaacccttgt
      541 tgtgggcctc accatgaata tcctttccat ctgcattttc cttgtgacct atggccgcat
      601 ggtggaaatc tatgttgtca cgtcgctggg gcctatcccc cttgccacaa tgggcaacgc
      661 ggaatggcgc ggcatgggcc aagggtatct gaaatccctg ctggcgctgg gcttccaagc
      721 ctttttaatc atggttgtgg tgggtatcta cgcggtgctg atacagaata tcggttccgc
      781 ttccgacata tccggcgcaa tctggggctg tatgggctat acggtgctgc tgtgcttctg
      841 cctgttcaag accggcagta tatcaaaggc cgtattcggg gcgcattgat cccaaaatcc
      901 aagcatacac aggaggaact tgtttatttc aacgtacaac atcatttacg ctgacccgcc
      961 gtggcggtat gactgcaaaa acgtacaggg cgcagcggaa aatcactatt ccaccatgag
     1021 cattgacgag ctgtgcgccc tgccggtgga acggctggcg tccaaagact gcctgctgtt
     1081 tttgtgggcc acattcccca tgctgccgga agctctgcgg ttaatcaaag cgtggggctt
     1141 ttcattcaag accgtggctt ttgtatggct gaaacagaac cgtaaaagcc ttacatggtt
     1201 ttacgggctg ggccgctgga caaggggaaa cgcggaaatc tgcctgctgg caacacacgg
     1261 caagccgaag cgtcgttctg cctctgtcca tcagtttatt atcagcccga ttgaacaaca
     1321 cagcaaaaag ccggatgtga cccgtgaaaa aatcgtagag ctggcgggcg atttgccccg
     1381 cgtggagctg tttgccagac agaaaacccc cggctgggac gtgtggggga acgaggtgga
     1441 atgtgacctt atcctgacgt gaaaggaggt gccaaatggc ttatgtaacc gtccccaagg
     1501 acttaaccca tgtgaaaagc aaggttgtgt ttgggcttac caaacggcaa ctgatctgtt
     1561 tttccggggc gctggtgatc ggcgtcccct tatattttct cacccgtgac tatcttacca
     1621 acagcgcggc ggccctgctc atggtatttg ccatgctgcc gggctttttg cttgccatgt
     1681 acgagcgtca cggccagccc cttgaagtgg tgattatgca gatcatccag tgctgcttca
     1741 tccagccaaa ggagcgtccc tatcagaccg acaacaccta caccgccctt gtgcggcaat
     1801 accagatgga acaggaggta aaggccattg tccaaaaagg aaatcccaag agaaaccgaa
     1861 aaaacaaagc tgacccgcgc ccagcgaaaa gaaattgacg cggtaatccg taaatataag
     1921 ggcgacggca ggccccacac ggcgcagcag agcatcccct acgaggtcat gtacccggac
     1981 ggcgtgtgcc gggtatcgcc cggcgtcttt tccaagtgca ttgagtttgc ggacatcagc
     2041 tatcagcttg cccagccgga cacgcagacg gctatctttg aaaaactgtg cgacctgtat
     2101 aactatgtgg acgcctccat tcatatccag ttttctttcc tcaatcggaa agttgacccg
     2161 gtgcagtacg ccaagagctt tgagatcgcg ccgcaggggg acgacttcga cgacatccgg
     2221 gccgagtaca ccggcattct gcaaaagcag cttgcaaacg gcaacaacgg catggtaaag
     2281 accaaatacc tgacattcac catcgaggcc gaaagcgtga aagcggcgcg agccagattg
     2341 aagcgcatcg gctttgacct gttaggctat ttcaagagca tgggggccgt agcccatgtg
     2401 atggacggct gggaacggct gaacctgctg catggcgtgt accacccgga cggcgagata
     2461 ttcaattttg actggaagtg gctggccccg tccggcctct ccaccaagga ttttattgcc
     2521 ccgtcctccc tgtgcttcgg caatgcaaag acattcggca tgggcggcaa atacggcgcg
     2581 gtaagtttcc tgcaaatcct gtccccggag ctgtcggacg atatgctggc cgacttcctc
     2641 aatacggaaa gcggtgtttt agtcaatctc catgtgcagg ccattgaaca gaccaaggcg
     2701 atcaagacca tcaagcgcaa aattaccgac cttgacgcta tgaagattgc ggagcagaaa
     2761 aaggccgtcc gcagcggtta cgatatggac attcttccca gcgaccttgc cacctacggc
     2821 gaggacgcca agaagctgct gacaaagttg cagacccgca atgaacggct gttccagttg
     2881 acgttccttg tcctcaacgt ggccgacacc aagcaaaagc tgaataacga cgtgttccaa
     2941 gccgcaggcg tggcccaaaa gcataactgt ccccttgtcc ggctggacta ccagcaggaa
     3001 cagggccttg caagcagcct gccgctgggc gtcaatcaga tcaagataca gcgcagcctt
     3061 acgacttcca gcgtggccgt gttcgtcccc tttgtgacgc aggagctttt tcagggcggc
     3121 gcggccatgt attacggcat caacgccaag tcccgcaaca tgattatgct ggacaggaag
     3181 caggcccgtt gccccaatgc cttgaagctc ggcacccccg gcagcggcaa atccatgagc
     3241 tgtaaatccg agatcgtcag tgtgttctta acgacgcctg acgatatttt tatttcagac
     3301 ccggaggccg aatattaccc ccttgtcaaa cggctccacg gacaggtaat ccggctgtcg
     3361 cctaccagca aggactttgt gaacccgctg gacatcaacc tgaattattc ggaggacgat
     3421 aacccgctgg ccctgaaatc cgactttgtg ctgtcgttct gtgagcttgt catgggcggc
     3481 aagaacgggc tggaagccat tgaaaagacc gtgatagacc gggctgtgcg cgtgatctac
     3541 cggccctatc tggcagaccc ccgcccggag aatatgccga tcctgtccga cctccacaaa
     3601 gcgttgttag atcagcatgt cccggaggct gaccgggtgg cgcaggccct tgacctgtat
     3661 gtgtccggct ccctgaacgt atttaaccac cgtaccaacg tggacatcgg caaccggctt
     3721 gtgtcctttg acattaagga gctgggcaag cagctcaaaa agctggggat gctcattgtc
     3781 caagaccaga tatgggggcg cgtcacggcc aaccgaagcc aaggaaaggc cacatggtat
     3841 tttgccgacg agttccattt gcttttgaaa gaggaacaga cggcggccta ttctgcggag
     3901 atttggaagc ggtttaggaa atggggcggc attccaacag gtgccaccca gaacgtcaaa
     3961 gacctgcttt cctccccgga aattgaaaat atccttgaaa acagcgactt cattacgctg
     4021 ctcaatcagg cgtccgggga tagaaaaatc cttgcggaac gcttgaattt gtccaccgaa
     4081 cagcagaagt acatcgacaa ttccgaaccc ggcgagggcc ttttgatctt tgaaaacgtg
     4141 gtgctgccct ttaccaaccc catcccgcac aacacccagc tttacaagat tatgacgacc
     4201 cgcttaaacg aggtggcggg agtatgaccc ggcgacgcca caaaggaggt gaaccaccat
     4261 gaaagagctg aaagccaagg cgaaagtgac gcaggccatg acccgcgacg ggctgacgct
     4321 ggaaaatcag gccaccggcg aggtggagaa catttccagc cgggaggccg aacaggattt
     4381 gtccgcgccg gggccgggcg gcaccgccga taagctgctg gaacgggccg acgccatcca
     4441 tgacggccat aaggccaaga aagccgcgaa gcaggccggg gctgtggccg ccgaggggga
     4501 cgctgccctg caccggccat ccgcccggct ccaactgtcg gaggaagaac gggccgaccc
     4561 ggatttacag ccctatatcc gaaaatcgga caaacgggcc gataagctgg ataaggcccg
     4621 cgccgccatc cccaagaagc gcgtcccggt caaggaaacc gtctttgacg aggcaagcgg
     4681 caaagcaaag accgtcctgc actttgacaa acaggacaag gggcctccct ccctgaaacc
     4741 caatccggca ggccgtcctg tttccgaggt ttttctattc ggccacggga aaatccatga
     4801 agtcgagcat gaaaacgtgg gtgtggaggg cggccataag ggggaggaac ttgccgagcg
     4861 gcaggccgga aaagccatcc gcagcaccat ccggcaccac aggctgaaac cctactggga
     4921 cgcggaaaag gccgagcgca ggctattgtc tgccaacgcg gagtatttct accagaaatc
     4981 tttgcgggat aacccggaga tcgcacaggc ggccagcaac cccatttccc ggttctggca
     5041 gaaacagcag atcaagcgcc agtacgccaa ggccgcccgg acagcgggcc agaccgcagg
     5101 cgcggcggca cagggcgcgg ccaagacagc cggggcctcc acgaaagcgg ccaaggccgc
     5161 agccaaagaa agtgaacggg cggcctcctt tgtggcccgc cactggaaag gggcgctggc
     5221 cgtccttgtg ttggggcttt tgatgctgct tatcatcggc ggcttacagt cctgcaccgc
     5281 catgttcggc agcgccggaa gcggcatcac ggcgtcctct tacttttccg aggatagcga
     5341 tatgttggcc gccgaggacg cctacgcgga gctggaagcc gagctgcaag actatctgga
     5401 caactacgag gccacccatg actatgacga ataccatttt gaccttgacg agatcagcca
     5461 tgacccctat gtgctgattt ccattctgtc ggcgctccat gacgggatat ttaccatcgg
     5521 ggaggtacag ggtgatttgt ccatgctctt tgacaagcaa tatatcctga cagaaaacgt
     5581 cgtggtagaa acccgttacc gcaccgaaac cgatacatgg acggacgcgg agggcaacac
     5641 ccatacagac acctaccaag tgccgtatga ctattacatc tgctacgtca cgctggaaaa
     5701 cttcgacctg tcccatgtgc cagtctatat catggacgag gaaacgctaa gtttctattc
     5761 cctgtatatg tccacgctag gaaaccggcc tgatctgttt tccggcagcc agtaccccaa
     5821 cgcctccacc ctgacgccgc cgacctacta cgacgtaccc ccggaggctt tggaggatgc
     5881 cacctttgcc gccatgctca aagaggcgga aaaatatatc ggttatccct atgtgtgggg
     5941 cggcagctct cccagcacca gctttgattg cagcggcttc atttcgtggg taatcaatca
     6001 tagtggatgg aacgtgggac ggctgggggc gcagggccta tataacatct gcacccctgt
     6061 ttccagttcg caggccaagc ccggcgacct tgtatttttc aagggaacct acgacacccc
     6121 cggcgtgtcc cacgtcggcc tgtatgtggg caacaatgtg atgctgcatt gcgggaaccc
     6181 gatttcctac acgaacctta cttcaaacta ctggcaatcc catttttaca gttacgggcg
     6241 tttaccttaa cgccagaaag gagctttcaa ttatggcaac caaacttgac cgcatcgaaa
     6301 aggacatcca gaaaaccaag tctaagatcg cggagtttca gaaacagctt cgggagctgg
     6361 aaacgcagaa aaccgaacag gagaatttgc agatcatcca gcttgtgcgc ggcatgaaca
     6421 tgaagccgga ggaatttgcc gcgttcttac gcagcggcgc aatgcaggcc gcccctgccg
     6481 caacccctta ccatgaacag gaggacaatg cccatgaaga ataagaccag acggatttcc
     6541 cgtattctcc ccgcacttct ggccgtgatg ctctgcgtca cggcctttcc cgttaccgcc
     6601 cttgcaggcg gcaatgaccc ggcaccggcc ccgctgccgg aggctacaac cgaacccgct
     6661 acgggcggtt tggagcctga aacagacgaa accggcaccg ccacggacgg gacagaaacc
     6721 gaggccgaaa aagataccgg ccctctgacc ggcaaggaca tcaccgacct gttttccgcg
     6781 ctgttcggtt ccaaggtgag cattgccgcc acggacgacg gcatccagat caccaccggc
     6841 accgacaaaa aagaacctga acagaccggc accgtcacca ccaacggcgg caggctgaat
     6901 gtccgcaccg gcgcggggct tgacaagacc gcgttcaccc agcttcccaa tgggacgacg
     6961 gttgaggtag tcggcacaga cggcgactgg attaaaattc tcttgccgga gcgcatcggc
     7021 tatgtccatt ccgactatat gaccgtcagc gaaaaggaag tagcggctac cggcgagggc
     7081 ggtttttccc tctccattga cccggaggaa attgcttctc tgctggaact gttcaatggc
     7141 ggcggggcca gcgccgccct gtccccggac gggaacctgt cactgattga cgacatcggc
     7201 agcgccgcga aaaatggcaa gcagtttatc accgtggaaa ccaaaaacgg caatgtgttt
     7261 tatctgatta ttgaccggga cgacgaggga aaagaaactg tccatttctt aaatcaggtg
     7321 gatgaagccg atctgctgac gctgatggag gacggggaaa ccgtcacccc cgctgctgtc
     7381 tgctcctgta ccacgaaatg caaggccggg gctgtgaaca cgaattgccc ggtatgtaaa
     7441 accaacctga ccgagtgcag cggcccggag ccgcaggaac cccagccgga ggaacccgaa
     7501 gccccggagg aagaacccaa gggcggcgcg ggcggcctga tcgtgttcct gctggtggcg
     7561 ctggcgggcg gcggtgcggc cctttactat ttcaagttca aaaagcccaa ggctgaaacc
     7621 accggcagcg acgatttggg cgagtatgat tttggggagg acgaggattt ggaggacgag
     7681 gaacccgaaa ccgagtttga cccggaagct gattttgtga gtgaacagga ggacgaggaa
     7741 tgacatataa acttgtaatc gcggaaaaac ccagcgtggc ccgcagcatt gcgggcgtga
     7801 tcggggccac ggagaaacac gacggctatt tggaggggaa cggctatctg gtgagctggt
     7861 gcatcggaca tctggtgtcc tttgccgacg ccggacgcta tgacgagcgt ttcaaaaaat
     7921 ggcgctatga ggatttgccc attctcccgg aaacatggca gtacatcgtc cccgacgaca
     7981 agaaacagca gtttgacgtt ctctgttcgc tgatgcagcg ccccgacgtg accggccttg
     8041 tgtgcgccac cgacgccggg cgcgaggggg aattgatttt ccgtttcgtc taccagatgg
     8101 cgggatgcca aaagcccttt gagcgccttt ggatttcctc tatggaggac agtgctatta
     8161 aggacggctt tgcccacctg aaaccgggcg gcgactatga tccgctgtat caatcggccc
     8221 tgtgccgggc caaggccgac tggctgatcg gcattaacgc cacccggctt ttttccgtcc
     8281 tgtaccacaa gaacctgact gtgggccgcg tccagacgcc gaccttaaaa atgctggtgg
     8341 acagggacgc caagattacc gatttcaaga aagagaaata tcacatcgtc catatcacgg
     8401 cgggcggcgc ggacgcggta agcagccgct tttccgacgc tgccgaggca aacaccgtca
     8461 aggcggcttg tgcaaaagcg caggccgttt gcgtttccgt gacgcgggag aaaaagacgg
     8521 aacagccgcc caagctctat gaccttacca ccttgcagcg ggaggctaac cggcttttcg
     8581 gctataccgc caagcagacc cttgactatg cccaggcact gtatctaaag aagctgctga
     8641 cctatccgag aacggatagt caatatctta ccgacgatat gctgcccgcc gtggaaagcc
     8701 ttgtgtccgg cctgtgggag ctggtgccgt ttgccaaggg cctgaacctt tccccgcagt
     8761 ttgaccgcat cctgaacagc aaaaaggtgt ccgaccacca cgccattatc cccaccgctg
     8821 aatttgtgaa gcagggcttt ggcactctgg ccgagagtga aaaaaagctg ctgtccctga
     8881 tctgctgcaa gctcttatgt gccgtggccg ggccgcatat ctatgaggcc gtcaccgcca
     8941 catttacctg tgccgggcag gagtttaccg ccaagggcaa gaccgtcctt tcccccggct
     9001 ggaaagagat tgaacgccgg ttccgttctt ccttaaagac cgacgccgac gaggacgccg
     9061 aggcggtgcg ggagctgccg gagctttgcg agggccagac ctttgcagac gtggccgcct
     9121 ctgtcaccga gcatttcacc acgccgccca agccctacac cgaagatacg cttttgtccg
     9181 ctatggaacg ggccggggcc gaggatatgc cggaggatgc cgagcgcaag ggattaggca
     9241 caccggccac ccgcgccgcc attctggaaa agctggtgca gatgggcttt gtccagagaa
     9301 aaggcaagca gctcattccc accaaggacg gcatcaatct ggctgtggtg ctgccggagg
     9361 cgcttacttc tccccagctt actgccgaat gggaaaaccg gctgaccgag atcgccaagg
     9421 ggcaggccga tcctgacgaa tttatggacg gcatcgagac gcaggcgcgg gagctggtgc
     9481 agacctattc ctgtatcagt gaggaaaagc agaaattgtt tcaggccgag cgtgttccta
     9541 tcgggacgtg tccccgctgt ggtgaggctg tctatgaggg caagaaaaac tactactgcg
     9601 gaaaccgggc ctgccagttc gtcatgtgga agaatgaccg attttttgag gaacgcaaga
     9661 aagcgttcac cccgaagatc gccgctgcgc tgctcaaaac cggcaaagtc aaggtaaagg
     9721 gcctctattc cgtaaagacc ggcaaaacct acgacgggac ggtgcttttg gctgataccg
     9781 ggggcaagta cgtcaactac cgcattgaac acaggagcta aaggacagta acacagcaag
     9841 catagggagt gtggctacac tcccggtaaa atcccccggc cgcgtccctg ccgggccggg
     9901 gatttttgct tgttggggaa gtccccaacc ccctattttt tgaaacagaa cagagaaagg
     9961 agtgaccgcg tgaacgaagt attttctatc caagtccata accggcagtt tttggaacag
    10021 ggcaaacaag gcgtctggct ggatttgccc accacaaccg aaacgctgca agccgccctg
    10081 cgggatgttg gcattactgc cgacaatccg caggattttt ttatcaacgg gtattcctgc
    10141 ccggaggaca gacaccttgc cattccctat gacatggtgc tggccgccga cgtggacgag
    10201 ctgaatttcc ttgccgcccg gctgggccag cttgacgccg ccgagatcgc agagctgaac
    10261 gccgccctgc aaaatccccg tggcggcttt gagaatatcg gccagatcat cgactacacc
    10321 gaaaatgtgg attactatgt gcatcttccc gacgttaggg gggatggaca gttgggcgac
    10381 tactacctga accgttccgg catggtggat atgccggagg aatggaaagc cgggattgat
    10441 acggcgcagt ttggacgcca cattgcccag caggaacaag gcgtttttac ccagtacggc
    10501 tatctggtga gaagcggcga cgaatggcag cgcgtccatg agggccagcc ggtgccggag
    10561 gaataccggg tgatgcacta cccccagccg gaagctgacc gggaggaacc gcaggcccag
    10621 cagccggagg catccgaaag ccccgacaaa gcgccgcccc cggttatccc gatcatgctg
    10681 gacggcatga acagcaaaga acgcatgaaa gtgattactg accgtctgga aaccggcgta
    10741 caggagcttt ttgaaagtag ccgctatatg gcttacttat ccgctatgtc caagatgcac
    10801 gattacagtc tgaacaatac gctgctgatc gccatgcagg gcggttcgat tgtggcgggc
    10861 tataacaggt ggcaggatga atttgaccgc catgtgaagc ggggcgagaa aggcattaag
    10921 atactggccc cggccccgtt caaggttaag cagcagatgg agaaaatcga cccggccacc
    10981 cagcagcccg tgatcggcgc ggacggcaag cccgtgaccg aagaacagga agtgacggtt
    11041 ccggccttta aggtggtgac agtctttgac atcagccaga ccgagggcaa ggattttccc
    11101 atgccctata tcagtgaact taccggcgac gtgcagcagt atcaggattt ctttgccgcg
    11161 ctggaacgga catcacccta tcccataagc attgagccga tttcagacgg ggccaagggc
    11221 cgctgcttct acgcaaaaca gcgcattgcc atcaacgagg gcatgagcga attgcagacc
    11281 ctaaagaccg ccatccatga gattgcccat gccaagctcc acgccattga ccgggaggcc
    11341 cccgccgagg aacaggcggc ccggccagac cgcagcaccc gcgaggtaca ggccgaaagc
    11401 gtggcctata ccgtctgcca gtattacggg ctggacacgt cggactattc ttttggctat
    11461 gtggccgatt ggagcagcgg cagggagtta tccgagctga aagcctccct tgaaaccatc
    11521 cgggccacgg ccagcgaatt gataaccgag atcgacggcc attttgccca gcttcagcag
    11581 gagcgcgagg cccggcagga acaggaggcc gtcacggttg acgttacccc ggaggcagcg
    11641 gccccggcgc aggccgagga agaacaggcc ccggcccttg atccggcagc cgagccgctt
    11701 gtgactgtgc tatggagcga aagcccccag ctaaaggacg gccagcaaat gccgttgcat
    11761 gaggccgacg cgctgtttgc ccgtctggat gcagcatatc cggcagaggc aggctatgac
    11821 aaaaccgcgt tccgcattga ctgtatcttt cagggcgagc cggacagcta cgaggggcgg
    11881 caggattttg gagataggga cggttccctg atcgaacata tccagagcta ccaccaattt
    11941 tacgcgcagg acgaaaactg gaaaaagcat gtaatcgcta caaccggcat agagggctgg
    12001 gagcaggaca aggcccatcg ggatatgctg ttaggcgagt ttgtccccta tatgaagctg
    12061 cattgcagcc ttgcccaaat ggaacagacg gcgcagggcc gcttacagtc cggggaaacc
    12121 ctgacgccgg aggaaacggc ctatttgggg gcgcttctct cttatgtgca ggagtgccgc
    12181 cccatgctca atcagggcca gtatcagttg ccggagccgc cgaaactggc tgacttcgac
    12241 cagagcttgc aggactacaa agcacaggta caggccgaga tcgcgcagga ggccgccgca
    12301 gccggtatga ccgtggagga atacgcggcg gctggctatg aagcacccca acaggacacc
    12361 ttttccatct accagcttcg aggcggggag gaaacccgcg actatcggtt tgagccatac
    12421 gagcgtttac aggccgcagg gctggccgtg gacagtgcca actatgacct tgtttatacg
    12481 gcaccgctgg ataaaagcac gtctttggag gatatttacc gcacattcaa catcgaccac
    12541 ccggcagatt ttaggggaca cagcctttcc gtttccgacg tggtggtgct gcaccagaat
    12601 ggacaggaca ccgcccacta tgtagacagc ttcggctata tggaagtgcc ggagtttttg
    12661 cgggaaaatc ccctgcgtac cgccgaactg tctacggagc agaacgaaaa catgattgac
    12721 ggcattatca acaatacgcc gcccgaaccg cagccggagg aacccgaaca gctatctttt
    12781 gaggatgtgg cagttgcttc cggccagaca caggaggaac ccccggaggc tgcgcccccg
    12841 caaacggctg tccgctatta tcccatcaat gagggagccg cccgccgcgc caaggaagcc
    12901 atgagctttt ctgactaccg gcccggcagc gccacggcgg aataccggca ctatgtcgat
    12961 caggccgtgg aaattgcgga gcgccagaaa aagcgtgtgg agccagagta tcacgaaaag
    13021 atagacagcc tccttgacac ctacgcccgc aagctggccg agaacatgaa taagggctat
    13081 gagattaccg cccgcgtccc ctctgtcctg attgccgggc cgtccaattt cccggtacgg
    13141 gcgaaagaaa agcagaacgc cgccagtgac cgcaacatgg aggaatttca gtacattcag
    13201 ggcttgcttg acaaaatccg cagcaccggc atgggtggta tcagcgccga cgatccgcag
    13261 gccgtttcca agctggaaaa gaaactggaa aagctggaag cgtcgcagga gttgatgaag
    13321 gccgtcaatg cctactaccg caagcatggg acgctggacg gatgccccca tctgaccgaa
    13381 agaggcattg aaaacctgaa agcggatatg gcgtcgggct ggcactatga gaaaaaaccg
    13441 ttccagtcct ggcagctctc caacaataac gccgagatac gccggttaaa gggccgcatt
    13501 gaggaattga cccgccaaaa ggaggcggcc tatgtgggat gggagtttga cggcggcact
    13561 gtggagatca accgggtggc caaccgcctg caaatcttct ttgaggggaa gccggatgct
    13621 gctgtacgcg acgagctgaa aagcaacggt ttccgctggt cgcccaaggc cggggcatgg
    13681 cagcggcagt taaacgacac cacaatccgc gtcgctgacc gtatcaagtg catccagccc
    13741 ctttccggcg aaaagcccag cgccttacag cttgcggccc gcagggagaa agaaaagccc
    13801 tctatccggg cgcagctcaa cgcggccaag accgaacagg ccaagcagcc accggcgcgg
    13861 gaaaaatcaa agggattgga ggtagagtaa atgcagaagt ttgaaaatgt ggacgttctg
    13921 gccgcgctgg aacagatcat gcggcagaac accgcgtttt atcaaagtga ctttggcatt
    13981 gacaaagaca ttatcaggcg ggatgccgcc agcgatcagg ccgcagacaa gaccctgctc
    14041 tggatgtccc ggccctccgg gacgtactgt ttccgggaac gtgatgtttt cctgaaagac
    14101 acccgacagt acaacacatg gaaattttac ggggagcaga cccgtgacaa gatactggcc
    14161 tacgcggtgg agctgaccgg cacacagggc ggcactatcc ggggcaatct ctatgagctg
    14221 gactatccgc agcatttccg gcacgtcatt gaaaccgccc agccggtgag tgtgaaccgg
    14281 ctcttttatg agcatggcac ccgtgacatc ccggagagcc agcgttttga cggaagccct
    14341 gaccgggtat tgggaaactt tttgtactat gaggcccagc cccatgaccc ggctgtgctg
    14401 caggaggcgc tacggacgga gcagcacagc cgggggcggg ccgtccccgg cgatttcaaa
    14461 gtccacgtca ccgctttgca tgacagccgg attgaaaccg aggcccggcg tattgtggac
    14521 agcctgaaag atctgtccgc gcctaacagc cccaataaaa cgcatttcat ggtggagatc
    14581 tccccgcact ttgcggcgat tgcttccacc aaggataatg accggctctt tgccatgctg
    14641 ccctataaat ccctgtgttt taccgggatg aaaggcaggc atggcgtgta tgccgtgatt
    14701 aaccgggacg agcgccggga tgtgagtatt cgcaggcccc ggccctctat ccgcaagcag
    14761 ctatccgaca gcaaacaggc cgccagccca aaaaaggcgg cggccagaac caaaaagaat
    14821 gaattggagg tataaggaat gatgcacttt accgtggagg aagaaaacct gatctgtatg
    14881 taccacaatg cagaccggcg cagaacgata aacaggctca ccgccgtcat gccggttatg
    14941 gatggggata tgcgggcgca ggcccagcag accctttcca agctggaacg catgacggat
    15001 gccgactatg acggccagaa gttccacttc acagacgaat aacgaacagc agacgccggg
    15061 cgcggcggcc atgtggtcgc ttgcgtccgg cgtcttttct ttttgcctat tcctacgcat
    15121 ttagaacccg gtattgtcgg ggataggatg aagtattcgc ttgcccctgc aaacatgcgg
    15181 gagaagcctt gcagcgggcg gttttcaatc ccttaaatgc gtaggttgca gctataatta
    15241 gacccctgaa acagatatag ccgcgcagcg ggtgtatctg tttcaggggt gtgcaggtga
    15301 tagccgcaaa gcggcgtaag agggtgcagc cctcttgccg tctatctctg ttccagcggg
    15361 aagctcggca atgcttccag cagtttcagg gaaatgcgct gccgtatatc ttcgtcaatg
    15421 actttcttct ttttgccgtt tgcctgtctg atctccaccg tggaaagctc gttgatgtag
    15481 tcagcataat gggccaacac tttttccgtg gcccacctct caccggcaac ggcggctttg
    15541 atcgtttcat agtccagcag cttttcttgt ccgtccatcc tgataccccc gtcagactga
    15601 ttttttctat gataccatat tcggaagcat ggaaccagag aaaacggcgc ttttctgctg
    15661 gtaaattcta cgcatttaga acagcacttc ggcggggata ggataaaaca cccacatacc
    15721 ccgctgacca tgccggaaaa gccttgaaat ataagggctt ttacagctct aagtgcgtag
    15781 gtttccagta tcttttccgc tggcgctcgg cctctttgcg gcggcgcatc cgtttggagc
    15841 aggccgcgca gtatttggcc cggttagact tcggcgtgaa cgtggccccg cactcacagc
    15901 agcgtttcac ttcgtcccgg tctttggtga tctcggcata cagggccgca tccagcggga
    15961 ggacagcggc cttaaaccag cggcacagaa gcgaatagga aatgctctgt acgcaaatac
    16021 attcttcgcc gtcgtccagc aacaggcagt tgccgtcaca atagttgcag cactcatgcg
    16081 tcagcttccg ggccttgcga tattgagggt aattcattcg tggaatactc atagctcttg
    16141 cgtccattcc tttcccggcg tcatacgcaa aatgccgtcc acattctgct tgatgatgcc
    16201 gtattcctgc atctgctttt tcgcttcgcc gtacttctca tacagccgtt ccttttcctc
    16261 ggccaatcgc ttgtactcgg ctttcagggc gtacagatcg gggagctttt tcacaccctg
    16321 tccctttagg gcctttgccg cagcttcata caaaatcaac gtgctttcat gctcacgccg
    16381 gaattttgcc ttatccgctg cattgcggta ttccatagcc acgggccaga gctgcttgta
    16441 ggtagaaata ttctttatca gcaccgccat atccgcaagc cggtgttcca tagctttgag
    16501 ggtagcggcg gcctcgtcgc tggcggcggt gatctcggcc actttgcttt ccaaatccgg
    16561 gtaggtgtct atcccatgtt cagtcaggaa attcaaagtc tttgcggcct gcttcaaatt
    16621 gtggagcttc gcccacctct catagcctgc cgactgctgg gccttaatgt tgttttccag
    16681 atcaatccgc aaagaaatcc ccttgcgctc tttgggcgct ctggtgataa cgcggccttt
    16741 gatacgctct ttgatggctt cctcggtgta ggcttccccc aatgtcttgc agcgggtaaa
    16801 gcgttcctgt ccgggagcgc ggaaagaaat aaactttccc ggcttgatct catagccctg
    16861 cgcctgcatg agccgcagga aatcatcaaa atcttttgcc tgcgggatag cggcgtcaat
    16921 ggctgctttg agcttcgctt tccagctcgt ccctttccgc tgggcgtccc actcggcata
    16981 gctcttgccc ttactctggc cgggcttcac cacggacaat ccgttttcct tgcacagcct
    17041 gtcgctggtg cgccggatat aggcatagct ctgtttgttg gaaatgtact tgcgctgatt
    17101 taccatatcc acggcacaga aaataatgtg attgtggacg tggcccttat caatatgggt
    17161 agtcagcaca tagggatatt tcccttgcag cacttcctcg gcaagctgct tcccgatctc
    17221 atgggcctgt tccggcgtgg cttcgccggg ggcaaaggat tgtatcaggt ggtggcccag
    17281 attattgccc ttttggtatg cctgcgccag cagcatttca aattcaatgt ccgctgtttc
    17341 ataagagcag gcaaaggagg ccaccagcag cttgccgtca gttttgtccg ggttctcgat
    17401 gtagtcaagg gcttttttca gcgttgactt gataggatgt atctttgtaa ctgccatatc
    17461 tgcgccagcg ccccctttat atcctcgatg tcctgtgcgt acaccggccc cataccgttc
    17521 agcctctttg caatctgatt gacgttgacg ccgatcttct gtatctcggc ggtctgcgcc
    17581 cggatgtcgc tggtgtccat gttgacgata tagccgtcaa tgagcatctt ccgggcgtag
    17641 gctgaaaagt tcttcgtatg cagcagcgcc attttctgtt caatcatagc ccgttcctcc
    17701 ggcgtcacag ggacgcgcag cacgattttt cgctttctgt ttgccatgct tcaccgtcct
    17761 ttccattcag gggtttgggg atttccccaa caagcaaaac ggacgcggcc cggcagggac
    17821 ggggcaggcc gtttttccgg gagtggctac actccctatg cttgctacgt tactgtttct
    17881 ccactaacaa gacgtttcca aagccccaaa ctacccgcaa aatagccctc tggaaagaaa
    17941 aaagtgcaaa aaaatagaag ctacgctgtg cagctcccgc taaaagtaaa acagaggatg
    18001 ccagttacac atcctccgtt tcattcttct tcggcttttc aattagtatg gcgctgtcaa
    18061 tcgcgccctc gatgatcggc aggtattctt cggggcagag tttcagcttg tggctgaccc
    18121 tttgccgcag ttcgctttcc tcccgcatga tctccgggtt aaagtagcgt tccacaggaa
    18181 gcccgcaggc tttaataagc tgtatgacaa ccggcagact gggaatggcc ccgtcgtttt
    18241 cgatgttggc aagatagcgc cagtcaatat ttattttctc tgccattgct ttgcgcgaaa
    18301 cgcgctttgc ttgccgggcc gctttcacat ccgcgccgaa agtttcaaag ccgggacaat
    18361 cttcaatatt cgccatatcg cacctcccac ttacattgta tatttcatac tctgcccgtg
    18421 gaatgttggt atatgcacct ttcttaaagt ttatatttca cattttgttt cttttattct
    18481 tcgagcaaaa gaattataat gtaccctgtg gaggtgtact atggactaca tgacgttgaa
    18541 agaggcaggc gagaaatggg gtgtgccccc acgatggata aactactatt gtgctgcggg
    18601 acgcatcccc ggcgctgtaa agatggcgac gatctggctc attcccaaaa ctgcggaaaa
    18661 gccgattgac aggcgtacaa agaaaggaaa ggcggttact gaatagtata atgaaaccgg
    18721 cgcattttgg atatggtttt gaagagagag aaagcatggt attttgtcac ggaaaaacta
    18781 taagatgata gatggcaggc tgctccagac cgataaaaag tattctcatc tgaaacagaa
    18841 agaaaagatt tacacatgga tgtatgaaga agcgaaactt tatcatgaca gaaatggaaa
    18901 atgcccgaaa caaagacgag gaagctgctg ctgtatcagc cgtatatggt agaattgaaa
    18961 aggccggcat ctggattctt tatggtgaag tctccagaca ttggataact gctggtctaa
    19021 tgcgcctttg agtatggggt gattgatttt aataccgggg aggtacggct ttttgaggaa
    19081 aatattctgg gatttacatt tggcgattat tacatttcct gtaactgcaa ggacacggat
    19141 gatgaagata tgaggaatat agacttgctg attttctact gtcttgaacg gtattaggac
    19201 tgaatctgct gcgcggagtg cggcaagagc gaccggaggg agtacacaca ggccctgcct
    19261 gtgtgaaaag ccataccgca aggtgcattc gcaaaaaaga ggccgcgcat tttgctgagg
    19321 cttggggtgt ccccaacaag caaaacgaga acgccgcagg cgggcaagtg gtggatcaga
    19381 atatccgtaa aggatattct gatccattgc ttgccagtat tatcgggatg ttttgacaac
    19441 cgctgtaatc gaaacatccc aaacattaag aaggtgttaa ttattattat cttgatttaa
    19501 ttcgtttaaa ccaataatat agatacatac accatgaaaa gaagcgagta tcccaatgac
    19561 ggatgattaa aatccgaaaa gaaatcgctt tgaaactaca gggaaactac agactgttat
    19621 gttatcttct taaaatggag ggatttttat gtcgatacga attctacttg tcgaggatga
    19681 tgatcatatc tgtaatacag taagggcgtt tctggctgag gcaggatatc aggtggatgc
    19741 ctgcacagat ggaaatgagg catacaccaa gttttacgaa aacacttatc aactggttat
    19801 tcttgatatt atgctgcccg gtatgaacgg gcatgaactt ttgcgtgaat ttcgtgcgaa
    19861 aaatgatact cccattctga tgatgacagc cctgtcggat gacgaaaacc aaatccgggc
    19921 gtttgatgca gaggcagacg actatgtaac aaagccattc aagatgcaga ttttactaaa
    19981 gcgggtggaa gccctgctgc ggcgcagcgg tgcgctggca aaggaaatcc gtgtcggcag
    20041 gctgacactt ctgccggagg atttcacggt actttgtgac ggtacagagc tgcccctgac
    20101 acgaaaagaa tttgaaatcc ttttgctgct ggtgcagaac aaaggcagaa ccttaacgca
    20161 tgaaatcatt ttgtcacgta tatggggata tgactttgag ggtgatggca gcacagtcca
    20221 cactcatatc aaaaatttgc gggccaagct gccggaaaat atcatcaaaa ccattcgcgg
    20281 tgtaggatac cgattggagg aatcataata atggaaagaa aagggatttt cattaaggtt
    20341 ttttcctata cgatcattgt cctgctactg cttgtcggtg taacagcaac actgtttgca
    20401 cagcaatttg tgtcttattt cagagtgatg gaattacagc aaacagtaaa atcctatcag
    20461 ccattggtgg aactgattca gaatagcgat aggcttgata ttcaagaggt ggcagggctg
    20521 tttcactaca ataaccaatc ctttgagttt tatattgaag ataaagaggg aagcgtactc
    20581 tatgccacac caaatgccaa tacatcaaat agttttagat ccgactttct ttatgtggta
    20641 catagagatg ataatatttc gattgttgct caaagcaagg caggtgtggg attgctttat
    20701 caagggctga caattcgggg aattgttatg attgcgataa tggttgtatt cagcctttta
    20761 tgcgcgtata tctttgcgcg gcaaatgaca acgccgatca aagccttagc ggacagtgcg
    20821 aataaaatgg caaacctgaa agatgtaccg ccgccgctgg agcgaaagga tgagcttggc
    20881 gcactggctc atgatatgca ttccatgtat gtcaggctga aagaaaccat cgcaaggctg
    20941 gaggatgaaa tcgcaaggga acatgagttg gaggaaacac agcgatattt ctttgcggca
    21001 gcttctcatg agctaaaaac gcccatcgcg gctacaagcg ttctgttgga gggaatgctt
    21061 gaaaatatcg gtgactacaa agatcattct aagtatctgc gcgaatgcat caaaatgatg
    21121 gataggcagg gcaaaatcat ttccgaaata ctggagcttg tcagcctgaa tgatgggaga
    21181 atcgtaccca tagctgaacc gttggacata gggcgcacgg ttgccgagtt gctgcccgat
    21241 tttcaaacct tggcagaggc aaacaaccag cggttcgtca cagatattcc agccgggcaa
    21301 attgtcctgt ccgatccgag gctgctccaa aaggcactat ccaatgtcat attgaatgca
    21361 gttcagaaca cgccgcaggg aggcgaggta cggatatgga gtgagcctgg tgctgaaaaa
    21421 tgccgccttt ttgttttgaa catgggcgtt cacattgatg atactgcgct tccaaggctg
    21481 ttcaccccat tctatcgcat tgatcaggcg cgaagcagaa aaagtgggcg aagcggttta
    21541 ggacttgcca tcgtacaaaa aacgctggat gccatgagcc tccagtatgc gctggaaaac
    21601 acctcggatg gcgttttgtt ctggctggat ttaccgctca catcaacatt gtaaatattt
    21661 aaaacttaaa tgattttgac cgacaggtat aaccctgccg gtcttttttt gctgctacag
    21721 gaaaactaca gactggctac agggaaagta cagatgcgct tgccataata acaatcgtac
    21781 cagccacaaa tcgtagtttt attgcaaagg aggcattcga tcaaatggaa aaaaaaagca
    21841 actatcattc caatgcggat catcacaaac ggcatatgaa acaatccgtg gaaaagcggg
    21901 cttttctatg tgcgttcatt atttcgttca cagtctgcac gctgtttttg gggtggagac
    21961 tggcttccgt attggaggca acacagatac cgcccatccc tgcaactcat acaggcagca
    22021 gcactgacgt agtggagaat ttggaggaaa acgctcttgc caccgccaaa gaacagggag
    22081 atgaacagga atggagcctg attttagtga acaggcagaa ccccatcccc gcacagtacg
    22141 atgtggagct tgagcaacta tcaaatggtg agcggataga tattcggatt tctccctatc
    22201 ttcaagattt gtttgatgcc gcaagaactg atggagttta cccgattgtc gcatccggat
    22261 accgaacaac agaaaaacag caagaaatta tggatgaaaa aattgccgaa tataaggcga
    22321 aaggctacac ctctgcacag gctaaagcgg aagcagaaac ttgggtggcc gtgccgggaa
    22381 cgagcgagca tcagcttggt cttgctgtgg atatcaatgc ggacggaatt cattcaacag
    22441 gcaacgaggt ttatagatgg ctggatgaaa acagctatcg ttttggtttt attcgtcgct
    22501 acccaccaga caagacagag ataaccggtg tgagcaacga gccgtggcat taccgatatg
    22561 ttggcatcga agctgccaca gagatgtaca accaaggggt ttgccttgag gaatatttaa
    22621 aaccagaaaa atgagaaaag gatgttatgt tatggacaga aaaagattga cacagcgttt
    22681 cccgttcctg cttccaatga gacgggcgca gagaaaaatg tgcttttatg cgggaatgag
    22741 atttgacggc tgtcgctatg cgcagacgat aggggaaaaa tcgctttccc atttgctctt
    22801 tgaaacggat tgcgcattat acaaccacaa taccggattt gacatgatat accaggaaaa
    22861 caaggtgttc aacttaaagc tggcagcaaa gaccttaaac ggcttattga taagaccggg
    22921 ggaaaccttt tctttctggt ggctggtacg ccatgcggac aaagataccc cctataaaga
    22981 cggccttacg gtgaccaatg gtaaactcac caccatgtcg ggcggcggta tgtgccagat
    23041 gagcaattta ctattttgga tgttcctgca tacgccattg acaattatcc agcgcagagg
    23101 tcacgaagta aaggagtttc cagagccaaa cagtgacgag attaaagggg tggatgcaac
    23161 catttcagag ggctggattg atttaaaagt gcgaaacgat accgactgca cctacccaat
    23221 atgggtgacc ttagatgatg agaaaatcat cggtcaggtg tccgccgaca aagagcccca
    23281 agcattatac aaaattacaa atggcagtat ccagtatgtc cgtgaaagtg gcgggattta
    23341 tgaatatgcc caggttaaac ggatgcaagt tgccttaggt accggggaaa taatagattg
    23401 caagctgctt tatacaaaca aatgcaaaat ctgttatccc ctcccggaaa gtgtggatat
    23461 tcaggaggag aaccaatgag aaaaagtgtg ggcattactg tttttggatg cgaacaggat
    23521 gaggcaaatg ctttccgcgc tttatcgccg gattttcata ttatccctac gctgattagc
    23581 gatgcgatat cggcagacaa cgcaaaattg gccgctggca atcaatgcgt tagcgtaggc
    23641 cataagtccg aggtttccga ggcgacaatt cttgcgctga gaaaggtcgg ggtaaaatac
    23701 atttctaccc gcagcatcgg ctgcgatcac attgatacga ctgccgccga gagaatggga
    23761 atctcggttg gcacggttgc gtattcgccg gacagcgttg cggattatgc tttgatgctg
    23821 atgctgatgg ccatacgggg tgcaaaaccc accatgcacg ccgtggcgca acaagatttc
    23881 agattggatc gtatccgggg gaaagaactg ggggatatga ctgtgggagt tattggaacc
    23941 ggccatatcg ggcaagcggt cgtcaaaagg ctgcggggat ttggatgcca tgtgctggcc
    24001 tatgataaca gccgaaaaat ggatgcagat tatgtccagc ttgatgagct tctaaaaaac
    24061 agcgatattg ttacgctcca tgtgccgctt tgtgcggata cccgccatct gatcggtcag
    24121 aagcaaattg gagagatgaa gcaaggcgca tttttaatca acaccgggcg cggagcgctt
    24181 gtcgataccg gggcgctggt ggaggcgttg gaaagcggaa agctgggtgg tgcggcactg
    24241 gatgtgttgg agggcgagga tcagtttgtt tataccgatt gctcgcagaa agtgcttgac
    24301 catccctttt tgtcgcagct tttaaggatg ccgaatgtga tcatcacacc ccatacggcg
    24361 tactacactg agcgtgtgct gcaggatact acagaaaaaa caatcaggaa ttgtctcaat
    24421 tttgaaagga gtttacagca tgaatagaat aaaagtcgca atcatcttcg gcggttgctc
    24481 ggaggaacat gatgtgtcgg taaaatccgc aatagaaatt gctgcgaaca ttgatacgga
    24541 aaaattcgat ccgcactaca tcggaattac aaaaaacggt gtatggaagc tatgcaagaa
    24601 gccatgtacg gaatgggaag ccgacagtct ccccgccata ctctccccgg ataggaaaac
    24661 gcatgggctg cttgtcatga aagaaagcga atacgaaaca cggcgtattg atgtggcttt
    24721 cccggttttg catggcaaat gcggggagga tggtgcgata caggggctgt ttgtattgtc
    24781 tggtatcccc tatgtgggct gtgatattca aagctccgca gcttgcatgg acaaatcact
    24841 ggcctacatt cttacaaaaa atgcgggcat cgccgttccc gaatttcaaa tgattgataa
    24901 aggtgacaag ccggaggcgg gtgcgcttac ctaccctgtc tttgtgaagc cggcacggtc
    24961 aggttcgtcc tttggcgtaa ccaaagtaaa cggtacggaa gaacttaacg ctgcgataga
    25021 agcggcagga caatatgatg gaaaaatctt aattgagcaa gcgatttcgg gctgtgaggt
    25081 cgggtgtgcg gtcatgggga acgaggatga tttgattgtc ggcgaagtgg atcaaatccg
    25141 gctgagccac ggtatcttcc gcatccatca ggaaaacgag ccggaaaaag gctcagaaaa
    25201 tgcgatgatt acagttcccg cagacattcc ggtcgaggaa cgaaatcggg tgcaggaaac
    25261 ggcaaagaaa gtatatcggg tgcttggatg cagagggctt gcccgtgttg atcttttttt
    25321 gcaggaggat ggcggcatcg ttctaaatga ggtcaatacc atgcccggtt ttacatcgta
    25381 cagccgctac ccacgtatgg tggccgccgc aggaatcacg cttcctgcac tgattgacag
    25441 cctgattaca ttggcgttaa agaggtgata agtatggaaa atggtttttt gtttttagat
    25501 gaaatgttgc atggcgtccg ttgggatgcc aagtacgcca catgggataa cttcacagga
    25561 aaaccggtgg atgggtatga ggtgaatcgc atcatcggca caaaggccgt ggcgtttgct
    25621 ctgcgcgagg cacaaatcca tgcggctgcg cttggctatg gcttgctttt atgggatgga
    25681 tatcggccaa gaactgcggt ggactgcttc ctgcgttggg cagcgcaacc ggaggacaag
    25741 ctcacaaaag aaaaatttta ccccaatata gagcgagccg agttgattac aaagggttat
    25801 gtggcctcac aatccagcca tagccgtgga agcgcaattg atcttacgct ctaccacctg
    25861 gatacagggg aacttgtttc aatgggaagt aacttcgatt ttatggacga acggtcgcat
    25921 catacagcaa aagggatagg ggatgcagag gcacaaaatc gaagatgctt gcgtaaaatc
    25981 atggaaagca gcggatttca gtcttatcgc tttgaatggt ggcactataa gttgattgat
    26041 gagccatacc ccgataccta ttttaatttt gccgtttcat aatgaaagta ttggttttgt
    26101 atcagatttt ggatttgata aaatctatgc tggtcatgga caatctttta ctggaaagtg
    26161 gttaaagcat cttttgtaat acattgcaaa ttatagtatt caatctgccc agcggcagaa
    26221 aagaaaaaaa ccgctgttgg gcagaccttt cccacgccca ttttgtattt tgctggcggc
    26281 ggttttgaga aatcaaggcc gccgttcttt tctggcagca aaaggtgtgg cggcccatag
    26341 gaggatgccg ctatgctgtt ttgctattgc catgctctaa tccacttcca ccactcaaaa
    26401 aaagccttgc ccctccacag ggctattccg gttatgagta tgtactatac catgtaaatc
    26461 agcagcatag caaagcattt tctcgcccgg taggtttgcg acctgccggg cgatttttgt
    26521 cgtcttttgc ggcccggctg tccccaaaaa aattttttga gaaattttca aaaaggaggt
    26581 atttagcggg tagctggcgg ccttgtcaaa gtcttgttag cggaaaggga gagaaccacc
    26641 taagtccccc acagaaaggg ggtgagaaga tggatgctat cccccgcgac tatgaagcgc
    26701 ggtgcatgtt cgatgctttt tgcaagacgg tactgcgtaa cgaggccaaa agctacttgg
    26761 ctgaaatgaa gcgccgccgt gaccgtgaag tgtcactaag ctctttgtca caggcagatt
    26821 tggacaagct ctgtacagta gatcactacc ccagcgacag ctttacgttt tcgtcgcatg
    26881 gctatgacct acatatcaac aatgagcttg ttgccgaagc ctttgccgcg ctgcccagca
    26941 tggagcagag tattttaatc ctgcactgtg ttctggatat ggcagatggt gagatcggcg
    27001 gccttgtggg aatgtcccgg agtgccgttc agcggcacag gacaaatacc ttatcggagc
    27061 tgcggaaaca gctaaaggcg ttgatgccga agggaggtta gacgcggatg aagaacccga
    27121 actacaaggg cattgagctt ttgccccttt cggtgatcga ggccgcccgc gctggggatg
    27181 ccggagccgt agagcgcgtt ttgcagtatt acagcggtta catgaacaag ctgtgtaccc
    27241 ggacgctcta tgacgaaaac ggctatccgc atgtgtgtct ggacgaggcc atgaagcgcc
    27301 gtctggaaat caagctcatc cacgctatcg tcacgaactg acgaaccggc cccggcagca
    27361 tgaaacggct taccctttcc ccgtttcggt gttttcccgg tggcctgatc gcaccttgat
    27421 aaatatgccc gcaagataac ggcggcgcat catagggcat acggacacat tctgtctgtg
    27481 ccgagccgga cggctggtgc gccatgatac ttttccctac gggaaaagcg gagcgaggaa
    27541 caccacgccg caatgtgagc gtggcggctt gcgggcgacg acgtacaggc aggactatga
    27601 tactccctta tagccacagt ccgagcgtta aaagcgtcgc aggcaatggg tagggccgcg
    27661 tgagaaccac gcgggggtga aagtcccgtg gagctgtgcc agcagccgtc cgtctatgcc
    27721 cctaaatcac acaaaccttt gaatttgtga aagggggctg cattatgaac catgatgtgc
    27781 ctatttggga aaaatacacg ctgacgattg aggaagcgtc taaatatttc cgtatcggag
    27841 aaaacaaatt gcgtaagctg gccgaggaaa atccggcctc tggctgggtc attatgaatg
    27901 gcaaccgcat ccagatcaaa cgcaagcagt ttgagaaaat aattgacact ttggacacaa
    27961 tctaatagtg aaaaagggcc gcagatatgt tataataatt tataagcata tatgcggctc
    28021 tttccgtcat ggaaaggagc atgacgagat gtcagaaaaa agacgagaca gcaaaaatcg
    28081 cgttttgcgg tctggagaga gccagagaaa agacggacga tatgcttaca aatatgttga
    28141 tacctttgga aaaccgcagt ttgtttactc atggaagtta gtgcctacgg ataagacccc
    28201 ggcaggtaaa cgggaggata tttcactccg ggaaaaggag aaagaaatcc agaaagacct
    28261 tgacgacggg atagacacca tcggcaagaa aatgaccgtc tgccagctct acgccaagca
    28321 gatcaggcac cggggcaatg tgaagcacaa caccaaactg ggccgggaac ggctgatgcg
    28381 tattctggaa caagatcggc tggggagctg cccgattgac agtgtgaagc tgtccgacgc
    28441 aaaggaatgg gcgcttcgga tgaaagaaaa gggcctttct tataagacca tcaataacga
    28501 caagcgttcc ctgaaagcgg ctttttacac cgccatacag gacgattgca tccgaaaaaa
    28561 cccgtttgac ttccagctta gtgacgttct ggacgacgat acggaaccaa aggtgcctct
    28621 tacaccagca caggaagaaa gttttctgtc ctttattcaa ggtgataagg tttaccaaaa
    28681 gcattatgac gcaatcgtta ttctgctggg gacagggctt cgcatttcgg aactgtgcgg
    28741 actgacggac aaagatttgg attttgaaaa ccgggtaatc atcgtgagcc accagctttt
    28801 gagaaatacc ggcgtcggtt actatattga cgaacccaag acccaaagcg gcgtcagaaa
    28861 aatccccatg aatgaggaag tgtatcaggc gttccagcga gtgataaaaa accgcaaagg
    28921 tgcaaagccg ttcattattg acgggtatgc caatttcctg tttctcaaac agaacggcta
    28981 tcccatgacg gcggtagatt atggcgggat gtttggcagg cttgtgaaga agtacaacaa
    29041 gagccatgaa gaagcgttgc cgaaaacgac gacgccccat gctatgcgtc atacattctg
    29101 cacccggtta gccaatgcgg gaatgaaccc caaagccttg cagtacatta tgggacattc
    29161 caatatcacc atgacgctga actattatgc acacgccaca ttcgattccg caagggcaga
    29221 aatggaacgg ctggcagcct gacacaaggc cggttttact actccattta ctacttttga
    29281 gggggaaatg gtgagaggta ataagaacat ttgtaagtat ctaacaatat caaaaatgcc
    29341 ggaaaagccc ggaaacacgg gctataccga cattcaagaa cttctaaaaa gataatctaa
    29401 aatcccatat aattttattt taaaatatgg gatattatga taaattaact atggatgata
    29461 tttgaaaaaa acatactgta tatttgattt aatttttatt tttaagctca tatcgtaaga
    29521 atgggacttg tagtctcact tctttatgga ggtgataatt atgaacgttg gttttattgg
    29581 agccggcaag gtaggttgtt cattcgggaa gtatttccag gaacataaaa tacaagtaac
    29641 aggattttat agtaaaagcg aagactcgtc attagcagct tcgaatttta catctagtaa
    29701 acaatattta aacttaagag aattagtaga tgaaaatgat actattttca ttacgactcc
    29761 tgatggtcaa attcaggagg tatggcaaga aatggttctg ttgcaaagtt ttaaatctac
    29821 tatcaaataa g
//
LOCUS       DAHHZJ010000384         7959 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.guided.129, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000384 DAHHZJ010000000
VERSION     DAHHZJ010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.guided.129"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            128..1564
                     /locus_tag="NBL02_003025"
     CDS             128..1564
                     /locus_tag="NBL02_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCD2573622.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="NBL02_003026"
     CDS             1753..4200
                     /locus_tag="NBL02_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCD2573623.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="NBL02_003027"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="NBL02_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HCD2573624.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5690..6793)
                     /locus_tag="NBL02_003028"
     CDS             complement(5690..6793)
                     /locus_tag="NBL02_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HCD2573625.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="NBL02_003029"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="NBL02_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HCD2573626.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2665 a         1359 c         1729 g         2206 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattactt
//
LOCUS       DAHHZJ010000385         5075 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.guided.174, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000385 DAHHZJ010000000
VERSION     DAHHZJ010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.guided.174"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..338
                     /locus_tag="NBL02_003030"
     CDS             <1..338
                     /locus_tag="NBL02_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HCD2573627.1"
                     /translation="KTGESRGILELLREGQQGVTLRTFESMYFQKKLVTDNQAIAHYQ
                     FYHSENIFLLQERDLDELPEFLHTPFQPIPSELLEFFDAENWAKRFFTTDSAIYEQYE
                     YDLSSVKNK"
     gene            complement(481..1422)
                     /locus_tag="NBL02_003031"
     CDS             complement(481..1422)
                     /locus_tag="NBL02_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCD2573628.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            1548..2669
                     /locus_tag="NBL02_003032"
     CDS             1548..2669
                     /locus_tag="NBL02_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCD2573629.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            2927..4429
                     /gene="eat(A)"
                     /locus_tag="NBL02_003033"
     CDS             2927..4429
                     /gene="eat(A)"
                     /locus_tag="NBL02_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HCD2573630.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            4457..5023
                     /locus_tag="NBL02_003034"
     CDS             4457..5023
                     /locus_tag="NBL02_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCD2573631.1"
                     /translation="MVKENFVNEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQALAEKVVDLPVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELISYE"
BASE COUNT         1650 a          913 c         1087 g         1425 t
ORIGIN      
        1 aaaaaaccgg ggaatcgagg gggattctag aactgttgcg ggaagggcag caaggagtga
       61 ctttgcgaac gtttgaatct atgtattttc aaaagaaact agtgacagat aatcaagcca
      121 tagctcatta tcagttttat cattctgaaa atattttttt gcttcaagaa cgtgatttag
      181 atgaattacc ggaatttttg catacgccat ttcaaccaat cccatcagaa ctgttggaat
      241 tttttgatgc agaaaattgg gcaaaacgtt ttttcactac tgactccgct atttatgaac
      301 aatatgaata cgatctttcc tctgttaaaa ataaataaaa gtgccaacca atcatagatg
      361 aaaataaaca agcgcaattc ttatggcaac atgagaattg tgtttttctt tttgaacttt
      421 catgggggta aaaaaatgag aaccagctgc tgatccagct gattctcatc agagagcctt
      481 ttattctgcg ccaaggattc cttcttttgc atggactaat tgggtcaaaa aggcacaata
      541 tggtgtagca atatcatatt tttgtccttt tcgccaaacg gcaccattga tgtagtcgat
      601 ttcagtcaaa cgatggtttt tgatcaggtc ttggtacata gatgggtaat gcaacccgat
      661 gttgtcagga tcataagtag aagcaatatg ttggtacact tcttcttgat ctaatgtgat
      721 tccttctttt gctgcgatag aagcaaactc agatacaatc gttcgaacca tgccctctgc
      781 cgctttttgc gcacctaatt cagcaatatt acaatccaat atcgtgcata atccatttaa
      841 tgttccgttt acacaagctt ttcgccaaat ggaataacga acattatgac tatagattgg
      901 attcaatttt gcttcttgaa agacttctac aacttttttt gtaaatgctt cgccttctgg
      961 ttccaagttc tctagttcga tttctccatc acctaataat gtgacttttc cgggaccagc
     1021 tagaccagct gtccacatcg tgatcccaaa taagatattt ttctttggta catatttttc
     1081 cagtacatct tcatgaccta atccattcag taaacacaaa acataagtat tctcagtaat
     1141 aatggattgg atcgaacgaa acatatcatc taattgattc gcttttgtca aggctacgat
     1201 caaatccacg tgtttgttgc tttcgatgat ttcttctggt gaatagattg gcagttttgc
     1261 aactacttct ttgccattaa agtcagcaat caatccattt gtacgaatgg cttcaatgtg
     1321 agcaggccag cgatcgatca agacgacctc atttccgctt tgatgcaaca ttaatccgat
     1381 tcggctgccc attgctccgg ctccagctat tgcgattttc atgttttttc tctcctttat
     1441 tcaatgagtt cccttttagt atatagaaaa acagaagaaa atagcattgt tttgtttgaa
     1501 aaaagtgaaa aaaagtgtct tacttaagaa gaaagtgagg gatatagatg gatattttgg
     1561 cggcaatcga ttcatttaaa ggaagtgctt cttcatttga attaaatcag gcagttctaa
     1621 atggttggga agaccgtaaa ggaagaaaaa tcaatgtgcc gattgcagat ggcggagaaa
     1681 atactgcaga agcaatctat tacgcgctcg ggggaacctg gcgtacgatt gatgggatgg
     1741 atctcttatt tcgtcagcga aaaatcagtt atttattgac gaactggcaa ggaaaaaata
     1801 ccgcagtcat tgaatctgca caagtattag gattagattt acttgataaa ccaacggatc
     1861 aaaccatccg acttgcttct agttatgcct tgggagagct tatcaaagat acactgcttc
     1921 aagaagtgga acaaatcatt gtcactttag gaggaagcgg atgtagcgat ggtggtctag
     1981 gattactcca atctttgggt gcttctttag aagggtacac agaaggaaat cctttgttat
     2041 ctactagaaa acttcatttg gatcgaagga acgttcttgt ttctaaagta aaagtgatca
     2101 tcgctgcaga cgtgaccaac ccatatacag gtcaacaagg agctgcttgg caatttggaa
     2161 gacaaaaagg aggaacggat acgacgttag ctttcttgga taaacaagct gaaaagattg
     2221 ctcagcagat aaaagatgtt tgggaggtgg atttagatga catcccagga agcggagcag
     2281 caggcggtat tggcggagcc ctttttttgt taggagcgaa gatggtctct ggttttgagt
     2341 tggtcagcca aatcactgga ttagaaaaac agatcgaaca agcggatgtc atcattacag
     2401 gagaagggcg gattgatcag caaacaatcc acggaaaagt tccatatggc gtagcagtgc
     2461 ttgcaaaaaa atatcgaaaa ccaataatcg ctatttgcgg aagtagagac cgagagctgg
     2521 aaggactctt tgattttctc cccattgtct taagcattca attaggacca atcgatttag
     2581 aggaagcaat ggaacacagg caaacattag aaaaaacaga aatcttagga cagacactca
     2641 gcagattttt caacattctc ccgaagtagt tgacaaaaac aatttcttta tgctatcgta
     2701 atttcaaata taggacaaag tgagggatcc gtgcattgaa aaattaagtc gattgatgaa
     2761 agctgcattc agactgtacc ttgtggagac aagggagcag tgtgcagatt ttcgggattg
     2821 gcttggttct ttcgatgtat gggtcttttt gtgtgcccca acccttgcat cctctttagc
     2881 cagttctgaa aataggactg gtcttttttt acgaggtgat ggaataatgt ctaaaatcga
     2941 aataaaaaat ctgacattcg gctatgacag ccaaggcaca ttattatttg aacaagccaa
     3001 tctaaacttt gacacacaat ggaaactagg acttatcgga cgaaacggtc gaggaaagac
     3061 aactttactg aatatcctgc aaaacaaact cccttatcaa gggcaagtga tccatcagca
     3121 agcgtttgtc tatttcccgc agcaaacaaa agataaagag cgtttgacct attatgtgtt
     3181 aaatgagatt atggattttg agatatggga aattgaaaga gagctccaac tgatgcaaac
     3241 agatcctgaa atcttatgga gagaattcag cacactatca ggaggagaga agacaaaagt
     3301 cctactggca ctcttatttg tggatgacac tcatttcccg ttaatcgatg aaccaacgaa
     3361 tcatttggat atctctggca gaaaacaagt agcggcttac ttgaaaaaga aaaaacaagg
     3421 attcatcgtg gtgagtcatg atcggggatt tatcgatgaa gtagtggacc atgttttagc
     3481 aatcgaaaag agccaactgg aactttatca agggaatttc tctatctatg aagaacagaa
     3541 aaaacttcgt gatgaatttg aaatagctaa aaatgaaaaa ttgaaaaaag aagtcagcag
     3601 gctaaagaaa acagcagctg aaaaagcaga atggtctcgt tcccgagaag gggacaaaac
     3661 aaagaaaaaa gtcggattca tcgatacgga atctaggcga gtgaataaag gagcagtagg
     3721 cgctgatgct gcacggacga tgaaacgatc taaagcaatc gtgaatcgga tggaaaccca
     3781 gatcagtgag aaagaaaaat tattaaaaga tatcgagtat atcgatgctt tgacgatgaa
     3841 tagccaagtg tctcatcata aacggctgtt aagcgtagaa aatcttcaat tagggtatga
     3901 aaatctgtta ttcgagccag ttcattttac gatcgaacct catcagcgga tggcaatttc
     3961 aggtcctaac ggcgcgggaa agtcatccat tatccattat cttctgggga cattcaacgg
     4021 caaggttgta ggagaaaaat cccagcccaa acatttgacc attagttacg tacgccaaaa
     4081 ttatgaagac aatcgaggga cattggcgga attcgcgaag aaaaaccgag tagactatca
     4141 agcatttttg aataatctcc gaaagcttgg gatggaaaga gatgtttttc ataataaaat
     4201 cgaacagatg agtatgggac aacggaaaaa agtggaattg gctaaatctt tatcccaacc
     4261 agctgagctc tatatatggg atgaaccatt gaattatttg gatgtcttca atcaagaaca
     4321 attagaacaa ctgattttga acgtgaaacc tgctatgtta ctagtggaac atgatcaaac
     4381 ctttctagat aaagtatcta ctgagattat ttctcttgag aaaatctaaa ccttgtaatc
     4441 tcaaagacaa caaggtatgg ttaaggagaa ctttgtcaac gaaggagaga atcacatgct
     4501 ttatgtaacg agacacggag aaacaacttg gaacgcacaa ggattagttt gcgggcgagc
     4561 agatgtgccg ctgactgaaa aaggacagat gcaggcacag gcactagcag aaaaggtagt
     4621 agatttgcct gttccaatca cgaaaatcat ccattcccct cttcaacgag caagagatac
     4681 agcacaagca gtagttgatc gtctcaattt gccgatgacc gctgatgagc gattggtaga
     4741 aatggatttt ggggattatg acggactgcc aagcaaagat gaaaactttc aaaaagcgcg
     4801 tttagcgttt gctgtacgtt tcccaaatgg agagtcggtc ttagatgtgt acgcacggat
     4861 cgttccttta ttgaaagaat gcatggaaga cgaagaaaat gtctacttgt tagtctgtca
     4921 taatgccttg ataagagtga tcaatgctta ttttcatcca atgcctaatg aagggttctt
     4981 tacattcatg gtagacaata cagaattaat ttcttatgag taattagact tttgtgttat
     5041 ttaaataaaa tgtttcaagg cactgctttt gctta
//
LOCUS       DAHHZJ010000386         4139 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.guided.195, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000386 DAHHZJ010000000
VERSION     DAHHZJ010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.guided.195"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            89..1567
                     /gene="msr(C)"
                     /locus_tag="NBL02_003035"
     CDS             89..1567
                     /gene="msr(C)"
                     /locus_tag="NBL02_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HCD2573632.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            1614..2060
                     /locus_tag="NBL02_003036"
     CDS             1614..2060
                     /locus_tag="NBL02_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HCD2573633.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2259..2774)
                     /locus_tag="NBL02_003037"
     CDS             complement(2259..2774)
                     /locus_tag="NBL02_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCD2573634.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            2891..4093
                     /locus_tag="NBL02_003038"
     CDS             2891..4093
                     /locus_tag="NBL02_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD2573635.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1229 a          704 c          899 g         1307 t
ORIGIN      
        1 tgtgatgcag tttttttgca aacagatttt tctgctcttt tttagtatcg ataattgttg
       61 ttttacatga aataggagtg taaaacatat ggaaaattta gcagtaaata taacaaatct
      121 acaagtcagc tttggcaacc agctagaact atctattgat tctcttcgtg tctatcagca
      181 agatcgtata gggatcatag gagaaaatgg tgtcggtaaa tcgactttgc tcaaactaat
      241 agccggtgaa ctttttcccg atcatgggaa aatccaaaca gagatcacct tcaactacct
      301 gcctcaatta acctatctcg ctgaagcaaa ggacctaaat ttggaattag ccagtcattt
      361 ccagttaaga ctggaagaaa cttcagagcg gaaatggagc ggaggggaag aacgaaagat
      421 cgagttgata cgtcttcttt cttcttatga acaagggatg cttctagacg agccaacaac
      481 ccatctagat agaaaaagta ttgatcgact gattgaagag cttcgttatt attatggtac
      541 gctggttttt gttagtcatg atcgctattt tctagatgag ctagcatcga aaatctggga
      601 agtaaaagac ggagaaatcc gagagttttc ggggaattat agtgcctatc tcactcaaaa
      661 ggaattggag aaaaagactc agctacgaga agcagagtcg atcatgaaag agaaaaaacg
      721 attggaaaaa tcgatccaag aaaagaaaaa acaagcggaa aagttggaaa aagtgtccag
      781 taaaaagaaa aagcaacaaa tcagactgga tcggttgtct tcctctaaac aaaaagacag
      841 tgtacaaaaa gccatccaaa agaatgcgaa aacattagag agaagactcc aaaaaatagg
      901 agaaacaacc aagccgcagc agatgaaaca aatccgtttt ccagtaccaa aatctcttga
      961 actccacagc cgctatccca tcatgggaca aaatgtccaa ttggaaagaa gtgggagaac
     1021 attactggta aatggtgatt ttcagttttc tttaggtaaa aaaatcgcga ttgtcggtga
     1081 aaatggttca ggtaagacaa ccttattaga acatatccgc aaacaaggag aaggaatcct
     1141 tctctctccg aaagtaagct ttcaagtata tcagcaaaag ggttatcaaa tgacatctga
     1201 agaatccatc attcgttttg tcatgagaca aacagagttt tcagaatcgc ttgtccgtag
     1261 tttgctgaat cacttagggt ttgctcagga aactttgacg aaaccgttat gtacattaag
     1321 tgggggagaa gcgacccgtc tgacgattgc tttgcttttt actaagccaa gtaatgtgtt
     1381 gctgttagat gaaccgacta attttattga tatggcaacg atcgaagctt tagagaagct
     1441 aatgcaaata tatccgggaa cgattttgtt tacttctcat gattcttact ttgtcgagcg
     1501 tacggctgat gaagtttatg aaataaaagg gcagaaaata aaaaaagtac ttacgagaaa
     1561 tttttaaatg tgctacgatg attttcgtga caaatttaaa ggagtggtac agattgccta
     1621 caaataaaaa agaaggaata atttttacaa caatgatgtg ttttttgatg gtcttgggga
     1681 tgagcgcgta taatttatgg ctgcatcaag atcttcattt atcaagtttg ttgattggtc
     1741 tagtgcctgg attcgtggtg gctttcattt tggatgtctt catcatcggt gtgctggcga
     1801 aaaaaattgc ctttaaacta ccagtaaatc cagaaagcaa ggtccaaatg atcttaacta
     1861 tttcatgctt gatggtttta ggaatggtga catgtatgtc tttattcggt gtgatcatgg
     1921 aacaaggaat acctgatcaa ttattctctg cttatatgaa tgcttggaaa atgaatgtga
     1981 ttgccgcttt accgttgcag ttgatcatcg ttggcccgct ttcaagaagt gtgctaaaag
     2041 caattcaaag taatgaatga tggaacactg ttatttgaaa aaaggccttg gcatgctttt
     2101 tgtcatagtc tcttgaatgc agttctagaa aaatagtgat ccgaacgata ggttttggcc
     2161 tgtcgttcgg atcgcttttt cttttccaac tgtattgctc ttttattgaa gataatttgc
     2221 tcttcaatag atatttctag aaaggttctg tttatccctt actcaaaaaa ctgctgcatt
     2281 tgttctctta cttgctgaca ccattggagt tcctgctgac aaagcataat tctgcgctgg
     2341 atcacaacat aacgtccaaa ttcttgtttc ttgtctagcg agaggtcatt caattttttt
     2401 tgattttttt gcatgatttt gttcagctgt tgttctctag tgaatagcag tccagtagct
     2461 gttgttttat cgagcgtcga aaaagcatag atttttgcta gaaattcatc tttttgcgta
     2521 ggagcgggag tttcctcttt gatccattcc tctacaagag cacgtccttc atcagtcaag
     2581 tggtagatct ttttttgaga gtgctcgtct ttgtccaaaa cggtcacata accctgctgg
     2641 tctaacttgg caagcgttgt atagatctga ctgtgatggg cttcccaaaa aattgtgatc
     2701 agctgcttca attcataccc agaacgtggt ttggataaaa gcacgcataa taatatatag
     2761 gctaaagtat tcataaaaat cctacctctc gtcttatttt ctttattata tcacagtaaa
     2821 gtttccacgt tttacatgtg catattgaca tgtaaaataa atcatgtatg atgctaatag
     2881 agagaaaggg atggaaatat tggagaatca aatagatata caagaacaaa caaatgaaga
     2941 aacaaagggg aaaagttcag cgctagttgt agcagctacc tgtatgatcg cttttatggg
     3001 agtgggtcta gtagacccaa tcttaaaaac gattgctgca cagttaaacg ctacgccagc
     3061 tgaaacgacc ctgttattta ctagttacat gttagtcact ggtgtggtga tgctatttac
     3121 aggtttctta tctagtcgga tcggtttgaa aaaaacattg atgacaggat tgtttatcat
     3181 cgttgtattc gctggtcttg gcggattatc tgggaatatc ggaatgctga ttgggttacg
     3241 tgccggctgg ggtgtcggga atgctttatt tgtctctact gctttagcaa cgatcgtcag
     3301 tattttagtt ggtaacacag aaaaagcaat catgatgtat gaagcagcac ttggctgcgg
     3361 aatggctgtt ggccctctag tcggtggggt cttgggcagt ggttcatggc gttatccatt
     3421 ttttggggta gcatttttga tgttttgcgg ttttatagcc ctagcggtac ttttacctga
     3481 aactgagaaa ccaaagagaa aagtaggcct ttgggaaggt gtcaaagcgt taagcaatca
     3541 taaacttcgt tcagttggac taatcgcatt gttatataat ttcggattct ttactttact
     3601 tgcgtatgcg ccatttttat tgattggcta ttctgaatta gaagtcggtt ttgtattttt
     3661 tggctggggt gtgcttctgg ctattgcttc gattttcgtt gcaccggctc ttgagcggaa
     3721 gtttacgact tatcggacaa ttttagcagc atttgtctta ttcattattt gtttgctgtt
     3781 attaggagct ggaacttcta tgccagtcct tgtaccgatc agcattgtgt tagcaggatt
     3841 tttccaagga attatcaata cactgttgac gacgattgct atggaaatac caggtctgca
     3901 acgaaacgtt gcttcgtcca gctatagttt tgtccgcttt ttcggtggag ctttagcacc
     3961 atttattgca ggtgtgattg gtgaacatat tggtgaaagc tatgcgtttt attttgcagg
     4021 tgccattgtt cttatcagtg tatgttgtat catttatcac cgtaattatt ttgctacaaa
     4081 tgaagaagaa taagaaatca gaggttgtga aaaagctgtc ctgcatcaag tcataagaa
//
LOCUS       DAHHZJ010000387         2020 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.guided.245, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000387 DAHHZJ010000000
VERSION     DAHHZJ010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.guided.245"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..82
                     /locus_tag="NBL02_003039"
     CDS             <1..82
                     /locus_tag="NBL02_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HCD2573636.1"
                     /translation="CMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            98..2017
                     /gene="tet(M)"
                     /locus_tag="NBL02_003040"
     CDS             98..2017
                     /gene="tet(M)"
                     /locus_tag="NBL02_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HCD2573637.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
BASE COUNT          689 a          315 c          412 g          604 t
ORIGIN      
        1 ttgtatgcct atggttatgc ataaaaatcc cagtgataaa agtatttatc actgggattt
       61 ttatgccctt ttgggttttt gaatggagga aaatcacatg aaaattatta atattggagt
      121 tttagctcat gttgatgcag gaaaaactac cttaacagaa agcttattat ataacagtgg
      181 agcgattaca gaattaggaa gcgtggacaa aggtacaacg aggacggata atacgctttt
      241 agaacgtcag agaggaatta caattcagac aggaataacc tcttttcagt gggaaaatac
      301 gaaggtgaac atcatagaca cgccaggaca tatggatttc ttagcagaag tatatcgttc
      361 attatcagtt ttagatgggg caattctact gatttctgca aaagatggcg tacaagcaca
      421 aactcgtata ttatttcatg cacttaggaa aatggggatt cccacaatct tttttatcaa
      481 taagattgac caaaatggaa ttgatttatc aacggtttat caggatatta aagagaaact
      541 ttctgccgaa attgtaatca aacagaaggt agaactgtat cctaatatgt gtgtgacgaa
      601 ctttaccgaa tctgaacaat gggatacggt aatagaggga aacgatgacc ttttagagaa
      661 atatatgtcc ggtaaatcat tagaagcatt ggaactcgaa caagaggaaa gcataagatt
      721 tcagaattgt tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga
      781 taaccttata gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga
      841 actttgcgga aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatat
      901 acgcctttat agtggagtac tacatttacg agattcggtt agagtatcag aaaaagaaaa
      961 aataaaagtt acagaaatgt atacttcaat aaatggtgaa ttatgtaaga ttgatagagc
     1021 ttattctgga gaaattgtta ttttgcaaaa tgagtttttg aagttaaata gtgttcttgg
     1081 agatacaaaa ctattgccac agagaaaaaa gattgaaaat ccgcaccctc tactacaaac
     1141 aactgttgaa ccgagtaaac ctgaacagag agaaatgttg cttgatgccc ttttggaaat
     1201 ctcagatagt gatccgcttc tacgatatta cgtggattct acgacacatg aaattatact
     1261 ttctttctta gggaaagtac aaatggaagt gattagtgca ctgttgcaag aaaagtatca
     1321 tgtggagata gaactaaaag agcctacagt catttatatg gagagaccgt taaaaaatgc
     1381 agaatatacc attcacatcg aagtgccgcc aaatcctttc tgggcttcca ttggtttatc
     1441 tgtatcaccg cttccgttgg gaagtggaat gcagtatgag agctcggttt ctcttggata
     1501 cttaaatcaa tcgtttcaaa atgcagttat ggaggggata cgctatggct gtgaacaagg
     1561 attgtatggt tggaatgtga cggactgtaa aatctgtttt aagtatggct tatactatag
     1621 ccctgttagt accccagcag attttcggat gcttgctcct attgtattgg aacaagtctt
     1681 aaaaaaagct ggaacagaat tgttagagcc atatcttagt tttaaaattt atgcgccaca
     1741 ggaatatctt tcacgagcat acaacgatgc tcctaaatat tgtgcgaaca tcgtagacac
     1801 tcaattgaaa aataatgagg tcattcttag tggagaaatc cctgctcggt gtattcaaga
     1861 atatcgtagt gatttaactt tctttacaaa tggacgtagt gtttgtttaa cagagttaaa
     1921 agggtaccat gttactaccg gtgaacctgt ttgccagccc cgtcgtccaa atagtcggat
     1981 agataaagta cgatatatgt tcaataaaat aacttagtgt
//
LOCUS       DAHHZJ010000388         1504 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.guided.272, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000388 DAHHZJ010000000
VERSION     DAHHZJ010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.guided.272"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            262..345
                     /locus_tag="NBL02_003041"
                     /pseudo
     CDS             262..345
                     /locus_tag="NBL02_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            394..477
                     /locus_tag="NBL02_003042"
     CDS             394..477
                     /locus_tag="NBL02_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HCD2573638.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="NBL02_003043"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="NBL02_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HCD2573639.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DAHHZJ010000389         1394 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.guided.274, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000389 DAHHZJ010000000
VERSION     DAHHZJ010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.guided.274"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            237..1391
                     /locus_tag="NBL02_003044"
     CDS             237..1391
                     /locus_tag="NBL02_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCD2573640.1"
                     /translation="MEYSEERQRLAYVRLYGGILHLRDSVRISEKEKIKITEMYTSIN
                     GELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRKKIENPHPLLQTTVEPSKPEQ
                     REMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQMEVISALLQEKYHVEIELKE
                     PTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSF
                     QNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVSTPADFRMLAPIVLEQVLKKA
                     GTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIVDTQLKNNEVILSGEIPARCIQEY
                     RSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRPNSRIDKVRYMFNKIT"
BASE COUNT          466 a          228 c          288 g          412 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaacct gtccggtaaa tcattagaag
       61 cattagaact cgaacaagag gaaatcagaa gatttcagaa ttgctccttg taccctgttt
      121 atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg ataacgaata
      181 aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc ttcaaaattg
      241 aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga atcctgcatt
      301 tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa atgtatactt
      361 caataaatgg tgaattatgt aagattgata gagcttattc tggagaaatt gttattttgc
      421 aaaatgagtt tttgaagtta aatagtgttc ttggagatac aaaactattg ccacagagaa
      481 aaaagattga aaatccgcac cctctactac aaacaactgt tgaaccgagt aaacctgaac
      541 agagagaaat gttgcttgat gcccttttgg aaatctcaga tagtgatccg cttctacgat
      601 attacgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa gtacaaatgg
      661 aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta aaagagccta
      721 cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac atcgaagtgc
      781 cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg ttgggaagtg
      841 gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcgttt caaaatgcag
      901 ttatggaggg gatacgctat ggctgtgaac aaggattgta tggttggaat gtgacggact
      961 gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca gcagattttc
     1021 ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca gaattgttag
     1081 agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga gcatacaacg
     1141 atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat gaggtcattc
     1201 ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta actttcttta
     1261 caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact accggtgaac
     1321 ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat atgttcaata
     1381 aaataactta gtgt
//
LOCUS       DAHHZJ010000390         1350 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.guided.278, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000390 DAHHZJ010000000
VERSION     DAHHZJ010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.guided.278"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            47..298
                     /locus_tag="NBL02_003045"
                     /pseudo
     CDS             47..298
                     /locus_tag="NBL02_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            391..1185
                     /locus_tag="NBL02_003046"
                     /note="aph(3')-IIIa"
     CDS             391..1185
                     /locus_tag="NBL02_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HCD2573641.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          425 a          234 c          322 g          369 t
ORIGIN      
        1 acattttcct ctaggaaaaa attaactagc accacgggtt atcttttata tagaagatat
       61 cgccgtatgt aaggatttca gggggcaagg cataggcagc gcgcttatca atatatctat
      121 agaatgggca aagcataaaa acttgcatgg actaatgctt gaaacccagg acaataacct
      181 tatagcttgt aaattctatc ataattgtgg tttcaaaatc ggctccgtcg atactatgtt
      241 atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa
      301 tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa
      361 atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg
      421 aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag
      481 gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat
      541 aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag
      601 ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg
      661 agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag
      721 attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt
      781 ccctatacga atagcttaga cagccgctta gccgaattgg attacttact gaataacgat
      841 ctggccgatg tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag
      901 ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac
      961 ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg
     1021 agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag
     1081 gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat
     1141 tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct agatttagat
     1201 gtctaaaaag ctttaactac aagcttttta gacatctaat cttttctgaa gtacatccgc
     1261 aactgtccat actctgatgt tttatatctt ttctaaaagt tcgctagata ggggtcccga
     1321 gcgcctacga ggaatttgta tcgataagaa
//
LOCUS       DAHHZJ010000391         1326 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.guided.283, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000391 DAHHZJ010000000
VERSION     DAHHZJ010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.guided.283"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            <1..82
                     /locus_tag="NBL02_003047"
     CDS             <1..82
                     /locus_tag="NBL02_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HCD2573642.1"
                     /translation="CMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            98..1294
                     /locus_tag="NBL02_003048"
                     /pseudo
     CDS             98..1294
                     /locus_tag="NBL02_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
BASE COUNT          474 a          199 c          261 g          392 t
ORIGIN      
        1 ttgtatgcct atggttatgc ataaaaatcc cagtgataaa agtatttatc actgggattt
       61 ttatgccctt ttgggttttt gaatggagga aaatcacatg aaaattatta atattggagt
      121 tttagctcat gttgatgcag gaaaaactac cttaacagaa agcttattat ataacagtgg
      181 agcgattaca gaattaggaa gcgtggacaa aggtacaacg aggacggata atacgctttt
      241 agaacgtcag agaggaatta caattcagac aggaataacc tcttttcagt gggaaaatac
      301 gaaggtgaac atcatagaca cgccaggaca tatggatttc ttagcagaag tatatcgttc
      361 attatcagtt ttagatgggg caattctact gatttctgca aaagatggcg tacaagcaca
      421 aactcgtata ttatttcatg cacttaggaa aatggggatt cccacaatct tttttatcaa
      481 taagattgac caaaatggaa ttgatttatc aacggtttat caggatatta aagagaaact
      541 ttctgccgaa attgtaatca aacagaaggt agaactgtat cctaatatgt gtgtgacgaa
      601 ctttaccgaa tctgaacaat gggatacggt aatagaggga aacgatgacc ttttagagaa
      661 atatatgtcc ggtaaatcat tagaagcatt ggaactcgaa caagaggaaa gcataagatt
      721 tcagaattgt tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga
      781 taaccttata gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga
      841 actttgcgga aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatat
      901 acgcctttat agtggagtac tacatttacg agattcggtt agagtatcag aaaaagaaaa
      961 aataaaagtt acagaaatgt atacttcaat aaatggtgaa ttatgtaaaa ttgataaggc
     1021 ttattccggg gaaattgtta ttttgcaaaa tgagtttttg aagctaaata gtgttcttgg
     1081 agatacaaag ctattgccac agagagagag aattgaaaat ccgctccctc tgctgcaaac
     1141 aactgttgaa ccgagcaaac ctcaacaaag ggaaatgtta cttgatgcac ttttagaaat
     1201 ctccgacagt gacccgcttc tacaatatta tgtggattct acgacacatg aaattatact
     1261 ttctttctta gggaaagtac aaatggaggt tctgttgcaa agttttaaat ctactatcaa
     1321 ataagg
//
LOCUS       DAHHZJ010000392         1178 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.guided.294, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000392 DAHHZJ010000000
VERSION     DAHHZJ010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.guided.294"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            8..913
                     /gene="ant(6)"
                     /locus_tag="NBL02_003049"
     CDS             8..913
                     /gene="ant(6)"
                     /locus_tag="NBL02_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HCD2573643.1"
                     /translation="MLYLKLCNRTLSLAEQDERIRIVTLEGSRANINIPKDEFQDYDI
                     TYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLTL
                     LPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNVT
                     PYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYISE
                     DLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRDM
                     YKKYTGKTGCLDSTYAADIEERREQ"
     gene            910..1158
                     /locus_tag="NBL02_003050"
     CDS             910..1158
                     /locus_tag="NBL02_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin acetyltransferase"
                     /protein_id="HCD2573644.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLLTRGAS"
BASE COUNT          409 a          169 c          257 g          343 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaaccctt tctttagcag aacaggatga
       61 acgtattcga attgtgaccc ttgaggggtc acgcgcaaat attaatatac ctaaagatga
      121 atttcaggat tatgatatta catattttgt aagtgatata gaaccgttta tatctaatga
      181 tgactggctt aatcaatttg ggaatataat aatgatgcaa aagccggagg atatggaatt
      241 attcccacct gaagaaaagg gattttccta tcttatgcta tttgatgatt acaataaaat
      301 tgatcttacc ttattgccct tggaagagtt agataattac ctaaagggcg ataaattaat
      361 aaaggttcta attgataaag attgtagaat taaaagggac atagttccga ctgatataga
      421 ttatcatgta agaaagccaa gcgcaaggga gtatgatgat tgctgcaatg aattttggaa
      481 tgtaacacct tatgttatta aaggattgtg ccgtaaggaa attttatttg ctattgatca
      541 ttttaatcag attgttcgcc atgagctgct gagaatgata tcatggaagg tcggcatcga
      601 aacaggcttt aaattaagtg taggcaagaa ctataagttt attgaaaggt atatatccga
      661 ggatttgtgg gagaaacttt tgtccaccta ccggatggat tcctatgaaa acatatggga
      721 agcattattt ctatgccatc aattgttcag ggcggtatcc ggtgaggtgg cggaaaggct
      781 tcattatgcc tatccggagt atgataggaa tataacaaaa tataccaggg acatgtataa
      841 aaaatacact ggtaaaaccg gctgcctgga tagcacatat gccgctgata tagaagagag
      901 gcgggaacag tgattacaga aatgaaagca gggcacctga aagatatcga taaacccagc
      961 gaaccatttg aggtgatagg taagattata ccgaggtatg aaaacgagaa ttggaccttt
     1021 acagaattac tctatgaagc gccatattta aaaagctacc aagacgaaga ggatgaagag
     1081 gatgaggagg cagattgcct tgaatatatt gacaatactg ataagataat atatctttta
     1141 acccgtggtg ctagttaatt ttaatatcct aaagaata
//
LOCUS       DAHHZJ010000393          700 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M95763
            SAMN28743207-rid14513643.guided.335, whole genome shotgun sequence.
ACCESSION   DAHHZJ010000393 DAHHZJ010000000
VERSION     DAHHZJ010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743207
            Sequence Read Archive: SRR19441809
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316799.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 10:51:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 24 of 62
            Pseudo Genes (incomplete)         :: 31 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743207-rid14513643.guided.335"
                     /isolate="M95763"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-05-15"
     gene            1..668
                     /locus_tag="NBL02_003051"
                     /pseudo
     CDS             1..668
                     /locus_tag="NBL02_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein"
BASE COUNT          251 a          112 c          137 g          200 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaacct gtccggtaaa tcattagaag
       61 cattagaact cgaacaagag gaaatcagaa gatttcagaa ttgctccttg taccctgttt
      121 atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg ataacgaata
      181 aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc ttcaaaattg
      241 aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga atcctgcatt
      301 tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa atgtatactt
      361 caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt gttattttgc
      421 aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg ccacagagag
      481 agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc aaacctcaac
      541 aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg cttctacaat
      601 attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa gtacaaatgg
      661 aggttctgtt gcaaagtttt aaatctacta tcaaataagg
//
